LOCUS       DATGBN010000001         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_466134, whole genome shotgun sequence.
ACCESSION   DATGBN010000001 DATGBN010000000
VERSION     DATGBN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466134"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VKL21_00005"
     CDS             complement(<1..866)
                     /locus_tag="VKL21_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HMB44180.1"
                     /translation="MDLITKIVKTIAWIIKTLAISGLFLIYPVLAAYPDMNLVANPGF
                     ESGTTAPLNWTFVTTNENTPVWSNISHSGSKSIEINIPGTTDLQSGYPESDRIIVQPF
                     TTYTFSAWGKTQEAGGTNMPTIILGELDADKNLLRLTNLPVFSRGTNDWEQKTVEFQT
                     GPNTSYIYIYANIWGGYGTFWMDDVTISLKTNLVVNPGFESGTTAPLNWTFVSQNGNT
                     PIWSDISHNGSKSIEISLPGTTDLQSGYPESEKISAQPFTAYTFSAWGKILEAGGTNM
                     PTVTFAELDADK"
     gene            1393..2061
                     /locus_tag="VKL21_00010"
     CDS             1393..2061
                     /locus_tag="VKL21_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB44181.1"
                     /translation="MNQVHLELKNVSKAVFRRVNNTRVRTQILSSLDLKVNKGELVTI
                     MGASGSGKSTLLRLVNRLSEADSGIILLNGNDIRSHVPMELRRRIGMVFQVPVTFKGS
                     VRDNLVFGIKLWGGNTGIETLSRDTGIPEDLLDADAGQLSTGEKQRVSIARALGNQPE
                     ILLLDEPTSSLDAVSAQRIEMLLLGLQKERDLTILWVTHENEQAERIGGRRLILRDGR
                     LDHA"
     gene            2039..2812
                     /locus_tag="VKL21_00015"
     CDS             2039..2812
                     /locus_tag="VKL21_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB44182.1"
                     /translation="MAGWIMLEVLAITFTVFLVILSAAYARRFGIGKEILLAGTRALV
                     QILMLASIILILFRKPLFWSFLVLMMMAAIAGHTTYSRSGKLEMGLPVAFVSITIASL
                     LLLIPFFLVGIFPPEARYIVPTGSILIGNAMNVSSLAIDRYKGEIKNRRAEVETYLAL
                     GARPEIAVKACLKQGILSALIPSIDNMKNLGIVWIPGVMTGMLLGGADPMEAASIQIA
                     IFTSIFATGMIASGLLLHFSTDSFFTKAAQLKEDMGQEL"
     gene            2903..3142
                     /locus_tag="VKL21_00020"
     CDS             2903..3142
                     /locus_tag="VKL21_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44183.1"
                     /translation="MEEEESKQIHKTLNDLIVKIDNKIDELSKSIEKTLGEKQEYAEG
                     KISENPLAYMAGAFVGGVIIGYMMGGGKSHCSKVK"
     gene            3263..>3996
                     /locus_tag="VKL21_00025"
     CDS             3263..>3996
                     /locus_tag="VKL21_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMB44184.1"
                     /translation="MNNQFEKIVPFVIFLLALFLFFKIIEPMITIFLSSILITYMFFP
                     LYKKVRTRISNQFLSIILTILVIVIVFSLPFSYVVIQVSQQSFEFYNSLSNNIAKGAL
                     FGIGCTSADSTICYLINNMEKISAQSLSKIGFDKQLQKLLPRLEDILTNYLIEIPLAI
                     LNGGFILFISYFLFKDGEKIIQKIAGWLPIRKKAFQKLIDQFEKVTHAVVFGQLFVAI
                     ALGFVAIIGFYIFGVPLPIFWGVIAG"
BASE COUNT         1171 a          747 c          875 g         1203 t
ORIGIN      
        1 ttcttatctg catccagctc tgcgaatgtg accgttggca tattcgttcc acccgcctcc
       61 aggattttcc cccatgcaga gaatgtataa gccgtaaatg gttgtgcgct tattttttcg
      121 gattctggat atccggattg aagatcggtt gttccgggga gacttatttc tatagatttg
      181 gaaccgttgt gggagatatc ggaccatatc ggggtgttgc cattctgact cacaaatgtc
      241 cagttcaggg gagctgttgt tccgctttca aagcccgggt ttactaccag attggttttt
      301 aaactaattg ttacatcatc catccagaaa gttccatagc cgccccagat attggcatat
      361 atgtaaatat aggaagtatt cggaccagtc tggaattcca ctgttttttg ttcccagtca
      421 tttgtacccc tactgaatac aggaaggttg gtcaggcgca ataaattctt atctgcatcc
      481 agctcgccta atatgatcgt tggcatattc gtgccacctg cctcctgggt tttcccccat
      541 gcagagaatg tataagttgt aaatggttgc acaattattc tgtccgattc cggatatccg
      601 gattgaagat cagttgttcc agggatgttt atttctatag attttgaacc gctatgggag
      661 atattggacc ataccggtgt attttcattg gtagtcacaa atgtccagtt caggggagct
      721 gttgtcccgc tttcaaagcc cggatttgca acaagattca tatccggata tgctgctaaa
      781 acgggataaa ttagaaagag acctgaaatt gcaagtgttt ttatgatcca tgcaatagtt
      841 ttaactattt tcgttattaa atccattttt tatctccgta ttcaatacat attatttaat
      901 atatattaaa taatatatcc tatccaatta tccaagttat gattaatcgt atatctacga
      961 acaagatatt tatagtttac gattggtgta aatataatgt cataattaat taatcaatta
     1021 aacatccaaa taacattcga taattaaaag atatcgatat ttttcacgca taattaatat
     1081 tatcaattac atttcccttt tggcttttgt tgaaaggtgg taaaataccc gaaatgaaat
     1141 cctgaaattg gttcattaca gcgcttgatt ctggcaaggt atggaaccag tccttagcga
     1201 tgcgatttca agggtgaaac aaactgcgat cggcatgaat tatcctgcca gcttttgatt
     1261 caataaaaga atccggagcc agagcggttc gctcgttaat ccagcaaaga ttataagtga
     1321 ggcttgttca taaaaaaaac gctgaacaaa gatatagtat tatttataga agtgtaaggc
     1381 atttaagtat tgatgaatca ggttcacctt gagttgaaaa atgtatcaaa agctgttttt
     1441 cgcagggtaa ataataccag ggtcaggaca cagatattaa gcagtcttga ccttaaggtc
     1501 aataaaggtg aacttgtcac tattatggga gcatcaggtt ccggaaagtc aactttgctt
     1561 cgtttggtaa acaggctatc tgaggccgat tccgggatca tcctgctaaa tggtaatgat
     1621 atcaggagcc atgttcccat ggagctgagg cgaaggatcg ggatggtttt ccaggtgcct
     1681 gttactttca aaggaagcgt acgggataac cttgtctttg gcataaaatt atggggggga
     1741 aatacaggaa ttgaaactct ttccagggat accggcatac ctgaagatct tctggatgcc
     1801 gatgcaggac aactctcaac aggggaaaaa caacgggtaa gcatagccag ggcacttggg
     1861 aaccagccgg agatcctctt gctggatgaa cccacttcat ctctggatgc ggtgtccgca
     1921 cagaggatag agatgctgct tttgggtttg cagaaggagc gggatttgac tatactatgg
     1981 gttacccatg agaatgagca ggcggaaagg atagggggaa gaagattgat cctcagggat
     2041 ggcaggctgg atcatgcttg aagttcttgc gatcacattt acagtcttcc ttgtgatact
     2101 gtcagcggcc tatgcgcgaa gatttggcat agggaaagaa atattactgg caggcacaag
     2161 agcactggtg cagatactga tgcttgcatc catcatcctg atattattcc ggaagcctct
     2221 tttctggagt ttcctcgttc tcatgatgat ggctgcaata gcagggcaca ccacctattc
     2281 aagatcggga aagcttgaga tggggttgcc agtggctttt gtttcgatta ccattgcgtc
     2341 tttattactg cttatccctt ttttcctggt tggaatcttc cctccggaag caagatatat
     2401 tgtccctacc ggcagcattc ttataggcaa tgccatgaac gtgagttcgc tggctataga
     2461 caggtataaa ggtgaaataa agaacaggcg ggctgaagtt gaaacatacc ttgcccttgg
     2521 cgccagacct gagattgcag ttaaggcatg cctcaagcag ggcattcttt cggctttgat
     2581 tccttccata gacaatatga aaaacctggg cattgtgtgg ataccaggag ttatgaccgg
     2641 aatgctgctt gggggagctg acccgatgga agctgcatcc atacagattg caatattcac
     2701 ttcaattttc gccaccggga tgatagcttc cggcctgctg ctgcatttct ccacggatag
     2761 cttttttacg aaggctgcgc agttgaagga agatatgggc caggagttat gacaatcaat
     2821 aacgctgact accggcgcaa cgctcgacta tcaagtatat atacagaaac ttacataata
     2881 tcacattgtg gaggttactt ttatggaaga agaagaatca aagcagattc ataaaacact
     2941 caatgactta attgtcaaaa ttgataacaa aattgatgaa ctatcaaaat caattgagaa
     3001 gacacttggg gagaaacaag aatacgctga agggaagata agcgaaaacc ctcttgctta
     3061 tatggcagga gcattcgtcg gcggggtgat aataggttat atgatgggcg ggggaaaaag
     3121 ccattgcagc aaagtaaaat agattttctt agcgttcctt tcagaatcga taattcaggg
     3181 ctttagcgaa catcgcaaca aaaggcggag actaaaaaag tttaattaac caaagaaatt
     3241 ggtttttgaa gaggaaagca ttttgaataa tcaatttgaa aaaatcgtgc cttttgtcat
     3301 attcttgctc gccctttttt tattttttaa aattattgag ccgatgataa ccatattttt
     3361 gagcagcatt ctcataacct acatgttctt tccgctctac aagaaagtaa gaacgaggat
     3421 atccaatcaa ttcctttcaa ttattctcac aatactggtt atcgtcattg ttttttcgct
     3481 cccattttct tatgtagtaa ttcaagtttc acaacagagc tttgaatttt ataattcgtt
     3541 gtcaaacaac attgcaaaag gagctttatt cggtatcgga tgcacgagtg ctgattcaac
     3601 aatttgttat ctaataaaca atatggaaaa aattagtgcg caaagtctgt caaagatagg
     3661 ttttgacaag caactacaga aactattacc gcgtttggag gatatactaa caaattacct
     3721 tattgaaata cctctggcaa ttctcaatgg gggttttata ttgtttatat cttattttct
     3781 tttcaaggat ggggagaaaa taatacaaaa gattgctggc tggcttccaa taagaaaaaa
     3841 ggcattccaa aagcttatag accaattcga aaaggtaacc catgcagtag tttttggaca
     3901 attatttgtt gcaatagccc taggatttgt ggctataata ggattctaca tttttggcgt
     3961 tccgttgcca attttttggg gtgttatagc aggatt
//
LOCUS       DATGBN010000002         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_116602, whole genome shotgun sequence.
ACCESSION   DATGBN010000002 DATGBN010000000
VERSION     DATGBN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116602"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VKL21_00030"
     CDS             <1..476
                     /locus_tag="VKL21_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB44185.1"
                     /translation="EIKTLIRQLELYEEQVELVEFKIDQSMKMIDSKIMSIPGIGDIL
                     GPIILGEIGNVDRFSSVKKLIAFAGLDPVVSQSGRFQNISGRISKRGSPLLRQALFLA
                     ANVARQNDDNLKRFYDKKKSEGKHHYSALNAVAAKLLRIVYWVLKNNKEYKTQVN"
     gene            799..1545
                     /locus_tag="VKL21_00035"
     CDS             799..1545
                     /locus_tag="VKL21_00035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB44186.1"
                     /translation="MTPEISVIVPVLNEAKYLESTLQTIVNQNTNVNYELIVSDNGST
                     DGSMDIAKKYADCVLQCEEIGIGAARHFGALQSSKTSKYLLFIDADTHIPGYYYAYLY
                     EIFKADPDLIAFSTGFKFSERSEQIKFAEKVANKYFIIRDKLRSATLPGFNTAVRRES
                     YFKCGGYRNVLLEDVDFSRRIDLLGAVKYFPHIKVINSSRRLEAMGLLGTLYYYTQLD
                     LGWELNATWIDKLTKKLGIADLREYIGIRK"
     gene            complement(1555..2418)
                     /locus_tag="VKL21_00040"
     CDS             complement(1555..2418)
                     /locus_tag="VKL21_00040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMB44187.1"
                     /translation="MKKKLIFLFILITLFSGCVGEKTVKTGDNISVDYTGSLLNGKVF
                     DTSIENVAKENNLSNHGNEYKPFKFTVGKGQMIKGFDEGVIGMKVGETRKLTIPPEKA
                     YGQSNPQMIQEIPILQEIPATKVLPKNFEVPVRQFESLLGPGHKKGENVMVPETNINL
                     TILNISSDNSSNVSLSYNLKMGDEVWTQGGTWNETVVKIDDKNITLRANVSENDIVQM
                     QGAPWNTTVVGLNNVNITLRHNPIQETVIPGRFGQMKVSFNETSIILDQNPELAGQTL
                     IFNVTIVSIDR"
     gene            2513..2734
                     /locus_tag="VKL21_00045"
     CDS             2513..2734
                     /locus_tag="VKL21_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44188.1"
                     /translation="MNGEKIMSISELEEEIIEIEKVKEHCIPGSMEWNNIDAIIAEKK
                     RKLSILKQGNSEEDYIPVCGGDCGGCGEG"
     gene            complement(2836..3144)
                     /locus_tag="VKL21_00050"
     CDS             complement(2836..3144)
                     /locus_tag="VKL21_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44189.1"
                     /translation="MKEQYRLTFALCVMALFVAAVAFGAISIINLTNNTTDENENETS
                     ATEIASQVAVAPVGTAVATPEETANEPAIPESTSNPVPGFGIMATIMFALFAIYLTHK
                     "
     gene            3467..>4541
                     /locus_tag="VKL21_00055"
     CDS             3467..>4541
                     /locus_tag="VKL21_00055"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMB44190.1"
                     /translation="MQILLIHSDFIEYETKKSTPVAEKIDDSLKKGRMEEALTVFMAV
                     EKNDDENLEYAALQTVSEIKKVTEQVKTNRVMLYPYAHLSSSLSSPKTAIETLKKIEE
                     LLKEAQFEVKRAPFGWYKSFTIKCKGHPLSELSRTIRIGEEKCITVIKEKEEVVSEAL
                     KSEAKATSYWHILTPDGELLKPEEFDFTGHGNLKKFVNYEIHKSRAVDKIPPHVELMR
                     RLELADYEPGSDSGNMRFYPKGRLMKSLIENYVLEETAKMGAMEVETPLMYDMNHPTL
                     KKYLDRFPARQYSIEADKKQLFLRFAACFGQFLMNHDMTISYRNLPLRMVELTRYSFR
                     KEQSGELVGLRRLRAFTMPDMHSL"
BASE COUNT         1422 a          873 c          949 g         1297 t
ORIGIN      
        1 ttgaaattaa aaccttgatc agacagcttg aattatatga agaacaggtc gaattagttg
       61 agttcaaaat agatcaatcg atgaaaatga ttgattcaaa aataatgtca atacctggta
      121 ttggagatat attgggtccg atcattcttg gtgaaatagg gaatgttgac aggttttcaa
      181 gtgtgaagaa attgattgct tttgctggac ttgatcctgt tgtgagccag tcaggaagat
      241 ttcaaaatat atctggacgg atctctaaaa gaggttctcc gctgcttcga caggctttat
      301 tcctggcggc taatgtggca agacagaatg atgataactt gaaacgattt tatgataaaa
      361 agaaaagtga gggaaagcac cattattctg ctttgaacgc agtggccgca aaattgctga
      421 ggatcgttta ctgggttttg aagaataaca aggagtataa aacacaagta aattaagttt
      481 aatctgttga atggggaaaa aaccaaacaa aatgatagca gatgctatct atttttctca
      541 ttaattacat gcaagtatag atgattgaat aatatgcaac caatgatttt aagttaaacc
      601 ccgccagcgg cggcgcatcc gctggggtat ggggtcgcac cccagcgccg ttaagataat
      661 gttgaccgaa agtgttaata gtacgctcat ctgcggtacg cagatttgtt gcatcggact
      721 tattcatcga tccttattca gaaaaatcgc acacacaaaa ccgttataag ctaatagagt
      781 cacaatcaaa taaagaatat gaccccagaa atttcagtaa ttgttccagt acttaacgaa
      841 gctaaatatc ttgaatctac acttcaaaca atcgtaaacc agaatacaaa cgtaaattat
      901 gaattgatag tttcggataa cggtagcacg gatggaagca tggatatcgc caaaaaatac
      961 gcggactgcg tgctccagtg tgaagaaatc gggattggcg cagccaggca tttcggcgca
     1021 ttgcaatcaa gcaagacaag caaatatttg ttgtttatcg atgcggatac ccatatacca
     1081 ggttactatt acgcttattt atacgaaata ttcaaagcag accccgacct catcgcattc
     1141 tctacaggtt ttaaattttc agaacggtcg gaacagataa aattcgcaga aaaagttgcc
     1201 aacaaatatt ttataataag ggataaattg cgttcggcaa cacttccggg ttttaacacc
     1261 gctgtcaggc gggaatctta cttcaaatgc ggaggttacc ggaatgtact gcttgaagat
     1321 gttgatttca gccggcggat agatctgctc ggagctgtga aatattttcc ccatataaaa
     1381 gttataaact catcacggcg tctggaagca atgggactcc tgggaacgct ctattattat
     1441 acacagcttg atctgggatg ggaactgaat gcgacatgga tagataagct tacaaagaag
     1501 ctggggattg ccgacctgcg ggaatacatc gggataagaa aatgattata tcgcctatct
     1561 gtcaatagaa acgatagtaa cattaaatat caatgtctgt cccgcaagtt ccgggttctg
     1621 atccaatatg atcgatgtct cattgaaact gactttcatc tgcccgaacc tgccggggat
     1681 cactgtttcc tgaattgggt tatgtctcaa tgtgatattt acattattca gaccaactac
     1741 tgtggtgttc cagggggcgc cctgcatctg aacaatatcg ttctcactca catttgccct
     1801 cagtgttatg tttttgtcat cgatcttcac aactgtctca ttccacgttc ccccctgcgt
     1861 ccatacttcg tctcccatct taagattata ggataatgaa acattagagg aattatcgga
     1921 ggaaatatta aggattgtca aattaatatt cgtttcaggg accatcacgt tttcaccttt
     1981 tttgtggccg ggccccaata aactctcgaa ctgtcttacc ggcacctcaa aatttttcgg
     2041 gaggactttt gtcgcgggta tctcctgcaa aatcggaatt tcctggatca tctggggatt
     2101 actttgacca tatgcttttt ctggtggaat cgtcaatttt cgggtttcac ccactttcat
     2161 tccaatcaca ccttcatcaa atcccttgat catttgtcct tttccaacgg tgaatttaaa
     2221 tggtttatat tcattgccat gattggaaag attattttcc tttgcaacgt tctcaatgga
     2281 cgtatcaaag actttcccat tcagaagact cccggtataa tcaacagaaa tattatcgcc
     2341 tgttttcact gttttttctc cgacgcaacc gctgaataat gtaatgagaa taaaaagaaa
     2401 aatcaatttt tttttcataa tgtcactatc acttctcaat gagaataacc tataagtaat
     2461 ttgcgatttc aaatactttg atcgataaaa taattacacc cggaaatcaa caatgaatgg
     2521 agaaaaaatc atgtcaataa gcgaacttga agaggaaatc atagaaattg aaaaagttaa
     2581 ggaacattgc attccaggta gtatggaatg gaacaatatt gacgctataa tcgctgaaaa
     2641 aaaaagaaaa ctcagcatac tgaaacaggg caatagtgaa gaagattata ttcctgtctg
     2701 tgggggagat tgcggtggtt gtggtgaagg atagtgacca atgccatatt ttaatttaat
     2761 aacttcttga tatcaaacta ttttagaacc aataataatt aaaattttta atagtgatag
     2821 aatatggagt taacctcact tatgggttag ataaattgca aagagagcga acataatggt
     2881 ggccattatt ccgaatccag gaaccggatt agaagttgac tccgggattg caggttcatt
     2941 agccgtctct tcaggggtag cgacagcagt acctacaggc gcaacagcaa cttgacttgc
     3001 tatttccgtt gcacttgttt cattttcatt ctcatctgtg gtattgttcg tcaggtttat
     3061 aatgcttata gcgccaaatg ctactgctgc aacgaataaa gccatcacgc atagagcaaa
     3121 tgttaatcta tattgttctt tcatgtttga tcttccatat gtttattggt tcttgcaata
     3181 tggtggtaga ggaagccaaa aaaaacattt tttttgtgga taggtcaggg ggtgaaaaac
     3241 ggcttcctca ttctccttgg agggcaacaa tcaatataaa taattcgata attattttga
     3301 gtttatttgt gtttttaagg aaattctgaa gcatatttca gtttataaaa gtttttgcat
     3361 gtagatgatg agggaagaat tcctgattga cctctgattc tatggaattc atgaaaccca
     3421 caaaacttta agcttaattg ctgtgtagat gctttaaagt gatacaatgc agatactact
     3481 tattcattcg gattttatag aatacgaaac caaaaagagc acccctgttg ctgaaaaaat
     3541 cgatgattca ctcaaaaagg ggaggatgga agaggctctt actgttttca tggctgttga
     3601 gaaaaatgac gacgagaacc tcgaatatgc cgccttgcag actgtgtctg aaataaaaaa
     3661 agtcactgaa caggtaaaga caaaccgcgt tatgttatac ccgtatgcgc atctgagttc
     3721 aagcctttca tccccgaaaa cagcgataga gaccctgaaa aaaatagagg aactgctaaa
     3781 agaagcgcaa tttgaagtca aacgcgcgcc ttttgggtgg tataaatcct tcaccattaa
     3841 atgcaaaggg catcccctct ccgaactatc aagaacgata aggataggcg aggaaaaatg
     3901 cattacggtt ataaaagaaa aggaagaagt tgtttcagaa gccctgaaat ccgaggcaaa
     3961 agcgacctca tactggcaca ttttgacccc ggatggtgaa ttgctaaaac ctgaggaatt
     4021 tgatttcaca gggcatggca atctcaagaa attcgttaat tatgagatcc acaagagccg
     4081 ggcggttgat aagatcccgc ctcatgttga actcatgaga cggcttgagc ttgcggatta
     4141 cgaacctggc tcagactccg ggaatatgcg cttctatccg aagggccgcc ttatgaagag
     4201 ccttattgaa aattatgtac tggaagaaac cgccaaaatg ggcgcaatgg aagtagagac
     4261 gcctttgatg tatgacatga accatcccac cttgaagaaa taccttgacc gcttccctgc
     4321 gcggcaatat tccatagaag ctgacaaaaa gcagttgttc ctgcgatttg cggcatgttt
     4381 tggccagttc ctcatgaacc atgacatgac aatatcctac aggaaccttc ctcttcggat
     4441 ggtggaactt acacgttaca gctttcggaa agagcaaagc ggggaacttg taggccttag
     4501 gcgccttcgc gctttcacga tgcctgacat gcattctttg t
//
LOCUS       DATGBN010000003         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_466193, whole genome shotgun sequence.
ACCESSION   DATGBN010000003 DATGBN010000000
VERSION     DATGBN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466193"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VKL21_00060"
     CDS             complement(<1..759)
                     /locus_tag="VKL21_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HMB44191.1"
                     /translation="MWLKLRLYLVMAVLFSIVYGLVVFAINKLGLPGTGFFFYGVLAT
                     IIMLIQYMIGPKMVEWQMGVRYVTEQEYPALHRMVSELARDARIPKPRIGIAMIPIPN
                     AFAFGRWAKDGRVCVTEGIMNLLNEKELRAVLGHEISHLKHKDVAVITMISVIPMICW
                     YFAWNQLFSGGRERGNGVLIGIVALIVYLITNLLVLYVSRIREYYADEGTVKLGNSPH
                     HLASALYKLVYGSARVSKESLKPIEGMKAFFANDP"
     gene            complement(782..1378)
                     /locus_tag="VKL21_00065"
     CDS             complement(782..1378)
                     /locus_tag="VKL21_00065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMB44192.1"
                     /translation="MSIASIAASLMLKEPKKRLQTFVFAQATGLFAIAATFYLMKCSE
                     MLSLYLYFAYVAISTAIIFGTLRYYDRLMIKRLEAKPAGSILKWTQEFVDKLTSARVY
                     YFDSAMPKAFAAGRSIFISIGLLELLDDDELKAVLAHEAWHIKHNSGMPFLSQLALMA
                     FSSSSEPELEERADRFAAKIAGSAALYSARNKVDKVFM"
     gene            complement(1453..1812)
                     /locus_tag="VKL21_00070"
     CDS             complement(1453..1812)
                     /locus_tag="VKL21_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HMB44193.1"
                     /translation="MVKLDQINISNDGLTKFFSPIEAAILRALWSEGEMTTSELTGKT
                     NIPLTSVAGTLDRLVKAGYTTRRTESINGRVRYVYEPVFSEEEAASEITTKILDSLVQ
                     AFGPLAVENFSKYSDKK"
     gene            complement(1838..2557)
                     /locus_tag="VKL21_00075"
     CDS             complement(1838..2557)
                     /locus_tag="VKL21_00075"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HMB44194.1"
                     /translation="MANPSIIGNDPGKSARPFVFINTAMSADGKIATIERRQTRISGS
                     LDFDRMDELRTSSDAIMVGIGTLISDNPSLTVKSKERKEKRRTRGLSENPPRIVVDSM
                     ARTPASSDIFLKGEGKRIIAVSKSAPAEKVKNLSKFADIICLGEKSVDLEKLLFELKK
                     RGINRLMVEGGATLNWELISNGLVDEIYTFVGNIIIGGKTAPTLVDGEGYTCDFSSLA
                     IISCERLEDGVLLKWKVNSKL"
BASE COUNT          750 a          623 c          477 g          813 t
ORIGIN      
        1 tggatcattg gcaaagaaag ctttcattcc ctcgatcggt tttaatgatt ctttagatac
       61 tcttgcgctg ccataaacaa gtttataaag agctgatgca agatgatgtg gtgagttccc
      121 aagttttaca gttccttcat cggcataata ttcccttatt cttgatacat acaggacaag
      181 aagattcgtt attagatata ctatcagcgc cactatccct ataagcactc catttccacg
      241 ttctctgccg cctgagaaca actggttcca cgcaaaatac cagcatatca tgggaattac
      301 gcttatcatc gttatgaccg caacatcctt gtgtttcaga tgggatattt catggcccag
      361 aacagccctg agttcttttt cattaagaag gttcattatc ccttcagtta cacaaacccg
      421 tccgtctttt gcccagcgcc cgaatgcaaa tgcattgggt ataggtatca ttgcaatacc
      481 tatccttggt tttgggatcc ttgcgtccct tgcaagttcg gaaaccattc tgtgaagcgc
      541 aggatattcc tgctcggtaa catatcgcac tcccatttgc cattctacca tctttggccc
      601 tatcatatac tgaatgagca taattatagt cgcaagaaca ccataaaaga aaaatcctgt
      661 tcctggaagg cccaacttat ttattgcaaa aaccacaagc ccatagacaa tggaaaataa
      721 cactgccatt acaagatata atcttaattt aagccacata tttaaccatc catatatttt
      781 attacataaa tactttatca accttattac gggcagagta aagtgcggca ctccctgcta
      841 tttttgcagc aaaacgatca gctcgttctt caagttcagg ctcggaagat gacgaaaatg
      901 ccatcaacgc aagttgtgaa agaaatggca ttccggaatt atgcttgata tgccatgcct
      961 catgcgccag tacggctttt aactcatcgt catccaataa ctcaagaagt cctattgata
     1021 taaagatcga ccgacctgct gcaaacgctt taggcatcgc ggaatcaaag taatatactc
     1081 tggcggatgt caacttgtcc acgaattcct gggtccattt caatatactt ccggctggtt
     1141 ttgcttcaag gcgttttatc attagcctgt cgtaataacg cagcgttccg aatattatgg
     1201 ccgtggagat agctacatat gcaaaataga ggtacaatga cagcatctcg ctgcatttca
     1261 taaggtaaaa ggtcgcagct atcgcaaaaa gacccgttgc ctgggcaaaa acaaatgtct
     1321 ggagcctttt cttcggttcc tttagcatca ggcttgctgc aattgatgct attgacatta
     1381 caaaaaataa actaataaag ggcgcccagg atctcaggca ctgtccataa cagtttattt
     1441 caccgaatat attcattttt tatcactata ttttgaaaaa ttctcaaccg caagaggtcc
     1501 gaatgcctgt accaggctgt caagtatctt tgttgtgatc tcacttgccg cttcttcttc
     1561 tgaaaaaacc ggctcataaa catatctgac ccttccattg atactttctg ttcggcgtgt
     1621 cgtatatcca gcttttacga gtcggtcaag agtacctgca acgcttgtca aaggaatgtt
     1681 tgtttttccc gtaagctctg aagtagtcat ctccccttca ctccataatg cccttagtat
     1741 tgcagcctct atgggactaa aaaattttgt gaggccatca tttgaaatat ttatttgatc
     1801 aagcttaacc atgcgtttac aattgacgta aaggatatta aagcttacta ttgactttcc
     1861 attttaataa aacgccatct tccagtcgct cgcatgagat aattgccaaa ctggaaaaat
     1921 cgcatgtata tccttcgcca tccactaaag tgggagccgt tttacctcct attatgatgt
     1981 tcccgacaaa agtataaatc tcatcaacga gtccatttga gatcagttcc cagttcaggg
     2041 tcgcgccccc ttccaccata agacggttga tacctcgctt ctttaattca aaaaggagtt
     2101 tttcaaggtc aacgctcttt tcaccaagac aaattatatc tgcaaatttt gaaagattct
     2161 taactttttc agccggcgcg cttttggaaa ctgcaattat gcgtttacct tcacctttta
     2221 gaaaaatatc tgaactcgcc ggggttcttg ccatgctgtc aaccacaatt cttgggggat
     2281 tttccgaaag tccacgggtg cgcctcttct cctttctctc ttttgatttt accgtaaggc
     2341 tcggattatc ggaaatcaaa gtcccgattc ccaccataat agcatccgat gatgtcctta
     2401 gttcatccat gcggtcaaaa tccagacttc cagatatccg ggtctgcctg cgttcaattg
     2461 tagcaatttt tccatccgcg ctcatggctg tatttataaa aacgaatggt cttgcagatt
     2521 tccccggatc attcccgatt attgaaggat ttgccatggt tctatctttg ccttcaaagc
     2581 taaaatattt aagcttaaac gaaaagctga aatgctaata gtccaattag accaaattag
     2641 ctggtgataa ttttgaagat act
//
LOCUS       DATGBN010000004         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_505170, whole genome shotgun sequence.
ACCESSION   DATGBN010000004 DATGBN010000000
VERSION     DATGBN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505170"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VKL21_00080"
     CDS             <1..287
                     /locus_tag="VKL21_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="binds guanine nucleotides; depletion results in
                     defective cell division; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMB44195.1"
                     /translation="EIVDRWTRRNEIPVEVELFEFFNELDIDVIVAANKMDKVTDQDL
                     ALDGVVQRLGMSPPWRQWPDKIVPVSAKKGNIGELKLLVQKKIEGTFFSR"
     gene            complement(302..1984)
                     /locus_tag="VKL21_00085"
     CDS             complement(302..1984)
                     /locus_tag="VKL21_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44196.1"
                     /translation="MITGRKNIRGIKWVVIFIVLLCFVSNAAAYEVNKLQWGIGKSGT
                     LKSNEVISSMGYTVEVVGFHGPVESDRYKGIPIEPVEAFVGLNLSRNGSFITQITLTQ
                     GESYVFPDGELRVTAVELPPGFSKDWLFESYNPWTKLELEPRGIPKPEILIESQDETV
                     SAPNTEIVVKVTLINTGSADLKNVNMDIGTELPVLSGNLKYHYEILKKGEEIRETITF
                     STPVVMDMKKNDIFVNVSGNDIKDNLYNSTKLKTILISPQAQKAPILRKTTVARMYLK
                     DQMLVSLNLKNNANYELNNVSIVDSIPKGFKQLTNNSLHWIVNIPANGEWNVRYLLKP
                     TEANSKGVLFPPSTAELKIMNEYYMIQSNRPDTVIYGARIELSKQPDVSEISQGDSVT
                     ITVVALNSGSTPSMVTINDTIPSNVELISGTTNLEKYLEANKDIRFSYTIRSNSEGAI
                     KLPRAIAEYYELGSEGTKVNTTSQELIIRIKSPQIPEPTPVPTVIIPVNSSGKVNTSE
                     EPAVNITHEQKSGNGPEPLSFDANAFLNLLIGCNNIIDPGFNLTSEVCSSIT"
     gene            complement(2103..3611)
                     /gene="lysS"
                     /locus_tag="VKL21_00090"
     CDS             complement(2103..3611)
                     /gene="lysS"
                     /locus_tag="VKL21_00090"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMB44197.1"
                     /translation="MTEETIHWADVIAQNVLSRGNKHTVASGITPSGPIHIGNMREVV
                     TADTVYRALLDRGADVRLIYIADTLDPLRKVYPFLSKDYEAHVGKPLSEIPCPCGSHK
                     SYSEHFLLPFIDALHTLGIKPDVYRADVLYKEGKYVEAIKKALINRDAIARILSEVAG
                     RELEPDWSPYNTICKECGRLSTTKVTGFDAEKETVDYQCKCHSSGTVSMKGGGKLNWR
                     VDWPARWPIFGITVEPFGKDHATAGGSYDTGKRIAREIYDYEPPFPIVYEWIMLKGKG
                     AMHSSTGLAISINDMLEIVPPEVLRYLIIRQKPEKHIEFDPGLSFLNLIDEYDQVEGD
                     KRAYQLSRTEGTKPSKIPFRHMVTAVQIAQDRDFEYLLTVLKRTDYDTSDVDAIRQRA
                     NNARNWLLKYAPIFVKFKVQENIPVQVKSFTKEQKLALLILADEIEHGMSGQEIHDNL
                     YRVAQETGLDGKQVFETVYLALLGLKSGPRAGHFLASLDKDFVVKRFKDVNI"
     gene            3680..4132
                     /locus_tag="VKL21_00095"
     CDS             3680..4132
                     /locus_tag="VKL21_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HMB44198.1"
                     /translation="MTRVAVGGTFDPLHDGHKALLMKAVELSRDGELLIGLTSDDMAR
                     NKIHEVNDYRSRYDELMKFIHYQGVFPQIVELNDPYGPTITDDFDYLVVSPETHSVGY
                     EINRMRSEKNLKPLKIILVDYVLADDGFPISSTRIGKGEIDNHGKVLR"
BASE COUNT         1166 a          894 c          869 g         1320 t
ORIGIN      
        1 cagaaatcgt tgacagatgg acccggagaa atgaaatacc agtggaggtc gagctttttg
       61 aattcttcaa tgaactggac atagatgtta tagtagctgc taacaaaatg gataaagtga
      121 cagatcagga tttggcactt gatggggtag tacaaaggct tggtatgtcg cctccatgga
      181 ggcagtggcc ggataagatc gtacctgtga gcgcaaagaa aggaaatatc ggggaattaa
      241 aactactggt acaaaagaaa atagaaggaa cctttttttc ccgttaactc tatatcaaag
      301 tctatgttat agagctgcaa acctcagatg tcaaattaaa tcctgggtca ataatgttat
      361 tgcaacctat tagaaggttc aaaaaagcat tcgcatcaaa ggaaagaggt tctggcccat
      421 taccagactt ttgttcatgt gtgatattaa cagcaggttc ctcgctggta ttgacctttc
      481 ctgatgaatt aacgggtatt ataacagtcg ggacaggagt gggctcaggg atttgcggcg
      541 atttaattct tattataagc tcctggctcg tggtattgac ctttgtacct tctgaaccca
      601 attcataata ttccgcaatg gcacggggca gttttatcgc cccttctgaa tttgacctta
      661 tagtataact aaatctaata tccttatttg cttcaagata cttttcaagg tttgtggtgc
      721 cgcttataag ttcaacattg gatggtatag tatcattgat agttaccatt gaaggggtac
      781 ttccgctatt taaagcaaca actgtaatag tcacagaatc accctggctg atttcggaaa
      841 catccggctg tttactgagt tcaatccttg ccccgtatat tacagtatcg ggtctgtttg
      901 actggatcat gtagtattca ttcattattt tcaattccgc ggtcgagggg gggaacaaaa
      961 cacctttact gttagcttca gtgggtttta agagatagcg gacattccat tctccatttg
     1021 ccggaatatt cacgatccaa tgcagggaat tattggtcaa ttgcttgaat ccttttggaa
     1081 tggaatccac aatactgaca ttattgagtt cataatttgc attattcttt aaattaagac
     1141 taactaacat ctggtctttc aaatacatcc tggcgaccgt tgttttcctg aggatgggag
     1201 ctttctgggc ttgtggtgaa ataaggattg tctttaattt agttgagtta tataaattgt
     1261 ctttaatatc atttccactg acgttcacaa aaatgtcatt tttcttcata tccataacca
     1321 caggtgttga aaaagttatt gtctctctga tctcctctcc tttcttcagg atttcataat
     1381 gatacttcaa atttccactt aatactggaa gttccgtgcc tatatccata ttaacattct
     1441 tgagatcggc tgatcctgta tttattagtg ttacctttac aactatttcc gtatttgggg
     1501 cagatacggt ttcatcttgg ctttctatca atatttcagg ttttggtatt cccctcggtt
     1561 ccaattctaa tttagtccag ggattgtaac tttcaaataa ccaatctttg gaaaaacccg
     1621 gaggaagttc cacggctgta actctaagtt ctccatccgg aaagacatat gattctccct
     1681 gcgtcaaagt gatttgtgtt ataaaactgc cattcctgga aagattcaat ccaacaaaag
     1741 cttcgaccgg ttcaattggt atccctttat acctatcaga ctcaaccggt ccatgaaaac
     1801 ctacaacttc taccgtatac cccatagatg aaattacttc attgctctta agagtcccgg
     1861 attttccaat accccactgg agtttattta cttcataagc agcagcatta ctgacaaaac
     1921 ataataatac tataaatatt actacccatt ttattcccct tatgtttttt cttcctgtga
     1981 tcatgttatt attggattgg attaacggta aataatacta tcctaaggat taacaaatcc
     2041 tgcatagtac ttaaatatta tgttcgtaga tatacgaata caagatgata aaatcataaa
     2101 atttatatgt ttacatcttt aaatcttttt accacaaaat ccttatccag ggacgccagg
     2161 aaatgccctg ccctgggtcc tgacttaagc ccaagaagcg ccagatatac agtctcaaat
     2221 acctgttttc cgtccagacc tgtttcctgg gcaaccctgt ataaattgtc atggatttcc
     2281 tgccctgaca tcccatgttc aatttcgtcg gcaaggatca atagtgccag cttctgttct
     2341 tttgtgaaac tttttacctg cacagggatg ttttcctgga ctttgaactt cacgaaaatg
     2401 ggagcatatt taaggagcca gttccttgca ttgttggctc tttgccttat tgcatctaca
     2461 tctgatgtgt cgtaatcggt gcgtttcaat acagtaagaa gatactcaaa atccctgtcc
     2521 tgggctatct gcacggcagt gaccatgtgc ctgaaaggga tctttgaagg ttttgtccct
     2581 tctgtccttg aaagttgata tgcccgctta tcgccttcca cctggtcata ctcatcaata
     2641 agattaagga aagaaagccc gggatcgaat tcaatatgtt tttcaggttt ctgcctgatt
     2701 atcaggtagc gcaatacttc aggaggcacg atttcaagca tatcgttaat ggagattgca
     2761 agacctgtgg atgagtgcat tgcccctttg cctttcagca ttatccattc ataaactatg
     2821 gggaatggcg gttcgtaatc gtatatctcg cgagcgatac gttttcctgt gtcatacgag
     2881 cccccggctg tggcatgatc cttaccgaaa ggttcaacag ttatgccaaa tatcggccag
     2941 cgcgcaggcc agtccacgcg ccagttaagt ttacctccac ctttcatgga aacggttccc
     3001 gacgagtggc atttgcactg gtaatccacg gtttcttttt ctgcatcaaa acctgtcact
     3061 tttgtggtac ttaaacgccc gcattccttg catattgtat tatatggact ccaatcaggt
     3121 tcaagttcgc gccctgctac ttcagatagt attcgcgcaa ttgcatccct gttgatcaga
     3181 gcttttttga tcgcttcgac gtattttccc tccttataaa gcacatcagc cctgtagaca
     3241 tccggtttta tcccaagagt atgaagcgca tcaataaagg gcaggaggaa atgctctgaa
     3301 tagcttttat ggctcccgca aggacaaggg atttctgaaa gcggctttcc cacatgggct
     3361 tcgtaatcct ttgaaaggaa tggatacact ttacgaagag ggtccaaagt gtcggcgata
     3421 tagataagac gcacatctgc gcccctgtca agaagcgccc tataaaccgt atccgcagtg
     3481 acgacttctc tcatgttccc tatgtgaatc gggccggaag gcgtaattcc tgaggccact
     3541 gtatgcttat tcccgcgcga aagtacattt tgggctataa catctgccca gtgtattgtc
     3601 tcttctgtca ttgtccccta aaatcgcata agagatatta aaggatactc ttatggttta
     3661 attctacaat catgagtaca tgacgcgagt agccgttgga ggcacttttg accctttgca
     3721 tgacggacat aaagccctgc tgatgaaagc tgttgaattg agcagggatg gtgagctact
     3781 gataggtttg acatcagatg atatggccag gaacaagata catgaggtga atgattatcg
     3841 atcgcgttat gatgaattga tgaaattcat ccattaccag ggtgtttttc ctcaaattgt
     3901 agagcttaat gatccctatg gtccgaccat aaccgatgat tttgattatc ttgtcgtatc
     3961 cccggaaacg cattctgtgg gatatgagat aaacaggatg cgcagtgaaa aaaatctgaa
     4021 accgttaaag atcattcttg ttgattatgt tcttgccgat gatggatttc cgatatccag
     4081 tacgcgtatc gggaaaggcg agatagataa tcacggaaaa gtattgaggt gataaaatta
     4141 acataagatt ccttggagga gcaagtgaag ttggacggtc cgccatcctg gtaaatgaag
     4201 agcttcttct tgattacggg ataaaaccta cggatccgcc gtcttaccc
//
LOCUS       DATGBN010000005         7149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_272152, whole genome shotgun sequence.
ACCESSION   DATGBN010000005 DATGBN010000000
VERSION     DATGBN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272152"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3677
                     /locus_tag="VKL21_00100"
     CDS             <1..3677
                     /locus_tag="VKL21_00100"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HMB44199.1"
                     /translation="GLANHSNEQYTRIIRGRFDIESDSQQNFDSKIQSILSNASGNNI
                     DPDHLVLSSSYLTDKSDDLSIMKYVQKFKGLNIHSSRIIVGIKNGKVVTLKSNYYPEK
                     DIDTETTLSKEDIIEIIEKDIGFQLIKGGLRPHETFSFEELEANKSAFVRKKFNLENR
                     TTVEDILLVIYPEPTRTEIKYHLTYKVELGLSISPPSKWVYFIDANNGEILEKYNKVV
                     FNILSGHVTGRIYPEYPNQSIVVRNFWNETLYDFTDHLSNIFWSGKNDNLDNYIFTKN
                     PISITNVSSATLEFKSKFLMENEHSFGYVVLSTDGRNYSNFEEYTGTKADWSTIKIDI
                     SSFIGQQIWIGFRYITDNINSDNGWYIDDIAVITNNGSIFKDSADNFTNWNEKGFSVI
                     KQEVKSNQELGKTGADGFYSLSGLQDSFTLSTGLAGEFVDVFNENGVDAKHYAIITTP
                     STHDIDWGNDDTSYRKEQSNVFYHINLAHDFFTKGSPFDIYAMNYQTHSYVQFPSTCG
                     AFSDGINIYLSGAGGGCEAASLFSDIIYHEYTHNVIDHVYTIHAPALPYRVESGALNE
                     GLADYFASTINGNHCIAEGLDGDCVRNLNNTYRFPEDVAGETHNDGKIISGSLWDLRK
                     MLGPDISDSLVINSMKLEPFNFSEYLDDIIVADDNNANLEDGTPHLFEICTAFFKNHG
                     IFSPYCSMYYRVPLKVNLLKNPGFESESANWTEYSNTGPIIKKYPMGTYSGKWLAYMG
                     NNNNVHQYIFQDLTIPSGIERAYVQFWYWIMSTETTEDSANDTMRIEIMRPDDDKLLK
                     SLGNLSNLNKSPGYRISEQYDISEFKNKTIRLKLTTTTDPFAPTAFLVDNIALMVVYD
                     TIPPDIDYVPPTPSNNSEMNVNYVNLSIVLNEPGGAAHLFWNGVLEDMDGSGLSFFKN
                     KTGLANDIYDYYVNASDIAGNFNTSEIRRVSVNVTAPGGHISGMKFNDSNGNGTKDPG
                     ESSLSNWTIVLKNSTGSTVETMMTDIDGNYTFGGLAAENYTVEEVLQADWKQTFPEAP
                     GKYKVTLAAGENVIGIDFGNNLPPLPSGINATREIENESLRPDESTTITVSIAGNANA
                     LALHEIPPEGWNVTRGTDNAEVFKNSTNEWVWKSMKANKTVTYTLTAPGNISIGTYQI
                     EGTIRNANGILANVEGDNSVKIDILEFYRRLGNDPAVVETIDILRAFDDFRNNIVPQG
                     FNRPLSAEEVDEPVNEWRNS"
     gene            complement(3806..5146)
                     /locus_tag="VKL21_00105"
     CDS             complement(3806..5146)
                     /locus_tag="VKL21_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMB44200.1"
                     /translation="MVNIVIIGLGSGGFAAFLAIRRTDPDAFITIIEKRPYQMFSPCG
                     MPYAIEGIINLDKLSFSLPEDKQSTKLLMHEAMSIDPIAKNVKTKNLGTGEILSIPYD
                     FLIIAQGAEPFIPPIKGAKENLGNGVYALHDIESANTISDRAGKVKKVAVVGAGPIGL
                     EIAVALRTRGLGVVVVEMLSFAMPRALDRDMAKLVERHLEAGGIKLIFDKSVKSINGP
                     PVNSVLVGGEIIETDMVILASGVRANFEIAKNAGIEHENWGIKTNAGMETNIKGIYAV
                     GDCIQTVSLINHRPAMMQLSSPAYRQGMVAGTNAAGGYDTYEGALGTFVTVLGNVEIA
                     ATGFNEFVAESAGYKIVSGKAHGKSKPEYYPGAKDITVKIIADARTGKVFGGQAVGEG
                     AGAFINVISLGIRCGIDVFSMARTEMAYCPMVGENYNVLNKAADFATRKLEKDR"
     gene            5345..6049
                     /locus_tag="VKL21_00110"
     CDS             5345..6049
                     /locus_tag="VKL21_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HMB44201.1"
                     /translation="MSDYTTGIKELDDAIGGIKKGSNIMLIGPPMSGKEAILNHILYH
                     GAVENGNAVITVTTRESAVHILDWFKEHKLNLPMERIGIIDCVTKTLGGPVVENESVK
                     IASSPVDLTGIGVKISQFFEEFFMKKNTQKIQLHINSLSTILMYSNIQTVFRFLHVFT
                     GRIKAASALGIYVIESGMHDEQAVATLKQLFDGMIEIKSEEDKNFIRVVGISQKPTPW
                     FEYEVQGHNVKIIRGN"
     gene            6051..>7149
                     /locus_tag="VKL21_00115"
     CDS             6051..>7149
                     /locus_tag="VKL21_00115"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44202.1"
                     /translation="MVETGIPKLDEFLGGGIPKGKSLVFYSQPGVEGEIFGLQTVHQT
                     LKMGETCVFVVSSTIPDIVKGQFKEFGWDISLFKDKLFFVDSYNPLIGAPSNEKYLIS
                     NPDKIEDFNKTIVDIIKELPPSTIVFGSLSTIMDLCGEKETIEAVRMWNKMAMLYNHV
                     IVYNFTAWPYSDETSDLIKGDLFNSVISIGGIAERVIFGQYFGILKSDWAQITRKSML
                     FRVLRPGGIKIFIPKILVTGPFDAGKSTFVHALSTRAISVDRLGTTIAMDHGHVDYKG
                     FSADIFGTPGQERFDPIIKLLSGESMGVFLVVDSTNPSDFIRAKHMLDITKSYGLPYV
                     VVANKQDLPGALTPEEIKEQFHLPEDVPIVPA"
BASE COUNT         2243 a         1367 c         1474 g         2065 t
ORIGIN      
        1 ctggattggc aaaccacagt aatgaacaat atacaagaat aatcagagga cgttttgata
       61 ttgaatctga ttcccagcaa aactttgact caaaaattca gtccattctg agtaatgctt
      121 caggtaataa catagatcct gatcatttag tgctgtcttc atcttatctt acagacaagt
      181 cagatgactt atcgataatg aaatatgtcc agaaatttaa aggattaaat atccactcaa
      241 gcaggataat tgttggaata aagaatggaa aggtagtgac gctcaagtca aactactatc
      301 ctgaaaaaga tattgacact gaaacaacgc tcagcaaaga agatattata gagatcatag
      361 aaaaggatat aggttttcaa ttaatcaaag gtggtttaag accacatgaa acctttagct
      421 ttgaagaatt agaggcgaat aaatctgctt ttgtcagaaa aaaatttaat ttagagaata
      481 gaaccactgt agaagatatc ttgcttgtga tttatcctga acccacaaga accgaaataa
      541 agtatcatct cacttataaa gtagaacttg gattgagtat atccccgcct tcaaagtggg
      601 tatatttcat cgatgcaaat aatggcgaaa tactggagaa atacaataag gtcgtcttta
      661 atattctgag tggacatgtt acaggacgca tatatccgga gtatcctaac cagagcattg
      721 tagtaagaaa tttctggaat gagacactct atgattttac agaccattta agtaatattt
      781 tctggtctgg aaaaaacgac aatttagata attatatttt caccaagaat cccatcagta
      841 ttacaaatgt ttcctcagca actcttgaat ttaaatcaaa gtttcttatg gaaaacgaac
      901 actcttttgg ttacgttgtt ctttccaccg atgggagaaa ctattctaat ttcgaagaat
      961 atacaggaac taaagctgat tggagtacaa taaaaataga tatatcttct tttatcggac
     1021 agcagatatg gattggcttt cggtatataa ctgacaatat taattctgat aacggctggt
     1081 atatagatga tattgccgtt attacaaaca atggcagtat tttcaaggat tctgcggata
     1141 actttacaaa ttggaatgaa aaaggatttt cagtaataaa acaagaagtt aaaagcaatc
     1201 aggaattggg gaaaaccggg gcagatggtt tttacagtct ttctgggcta caggatagct
     1261 ttaccctttc cacaggactt gcaggggaat ttgttgatgt atttaacgaa aatggggtgg
     1321 atgcaaaaca ttatgcaata attaccacac catctactca tgacatagat tgggggaatg
     1381 atgatacttc ttatagaaaa gagcaatcaa atgtttttta tcatatcaac ttggcccacg
     1441 acttcttcac caaaggaagt ccatttgata tctatgcaat gaactatcaa actcattcat
     1501 atgtgcaatt tcctagtaca tgcggtgcat tttctgacgg cataaatata tatttatcgg
     1561 gtgctggagg gggttgcgag gcagcttcgc tgtttagtga tattatctat catgaatata
     1621 cccacaatgt aattgatcat gtgtatacaa ttcatgcacc agcgctacct tatagagtcg
     1681 agagcggtgc attgaacgag ggattagcgg attattttgc gagtactatt aatgggaatc
     1741 actgtatagc ggaaggtttg gatggtgatt gtgtacgaaa tctgaacaac acttatagat
     1801 tccctgaaga cgtagctggt gagacgcaca atgatggtaa aattatatcg ggatctttat
     1861 gggatctcag aaaaatgtta ggtcctgata tatcggattc tcttgttatc aattctatga
     1921 aacttgaacc ttttaatttc tccgaatacc tcgatgatat tatagttgca gatgataata
     1981 acgcaaatct cgaagatggt accccacatc tttttgaaat ctgtaccgct ttttttaaga
     2041 atcatgggat tttctctcct tattgcagca tgtactatcg ggttccactg aaagtgaatc
     2101 tattgaaaaa tccaggattt gagagtgaaa gtgccaactg gactgaatac agcaatacag
     2161 gccctattat caagaagtat cccatgggaa catatagcgg aaaatggctc gcctatatgg
     2221 gtaataacaa caatgtgcac caatacattt ttcaggattt aacaattcct tcagggattg
     2281 aaagggcgta tgtacaattc tggtattgga ttatgtcaac agaaacaaca gaagactctg
     2341 cgaatgatac aatgagaatt gaaataatga gacctgatga tgataaacta ttgaagtctc
     2401 ttggaaacct gagtaatctt aataaaagtc ctgggtatag aataagtgaa caatacgata
     2461 tatcagagtt taaaaataaa acaatcaggt tgaagctaac tacaacaacg gatcctttcg
     2521 ctcctactgc ttttttggtg gataatattg ccttgatggt tgtttatgat acaattccac
     2581 ctgatattga ttatgtacct ccgacaccat ctaataattc agaaatgaac gttaactacg
     2641 tgaatttgag catcgtgcta aacgagccag gcggtgcagc gcatctattc tggaacggcg
     2701 tcttggaaga tatggatggt tcaggtctca gctttttcaa gaacaagaca gggcttgcca
     2761 atgatatcta tgactattat gtgaacgcaa gcgatattgc aggtaatttc aataccagtg
     2821 agataaggag agtatctgta aatgttacag cacccggagg ccatatctca ggcatgaagt
     2881 tcaatgactc aaatgggaat ggaacaaaag atccagggga atccagcctt tcgaactgga
     2941 cgatcgtgct gaaaaacagc acaggatcta cggtggaaac catgatgaca gatattgatg
     3001 gcaattatac cttcggtggg ctggcagcag agaattacac cgtggaagaa gtccttcagg
     3061 cagattggaa gcaaacattc ccggaagcgc ccggaaaata caaagtgact ctggcagccg
     3121 gggaaaatgt aattggaata gactttggca ataatctgcc tccactacca tccgggataa
     3181 atgctacacg tgaaatcgag aatgagtctc tccgcccgga tgaatcaaca actattaccg
     3241 tcagcatcgc cgggaatgca aatgcattag ctcttcatga aattcctcca gagggatgga
     3301 atgtaacaag aggaactgat aatgcggaag ttttcaagaa cagtacaaat gaatgggtat
     3361 ggaaaagcat gaaagccaat aagactgtta cctatacatt gacagcaccc gggaacattt
     3421 ctattgggac ttatcagatc gaaggaacaa tacgaaatgc aaatggaata ttggcaaatg
     3481 tagaaggaga taatagtgtt aaaatagata ttctggagtt ctacagaaga ctcggtaacg
     3541 atcctgctgt agttgagaca atagatatat tgagagcttt tgatgacttc agaaataaca
     3601 tagttcctca aggatttaat cgaccattga gtgcagagga ggtagatgaa ccggttaacg
     3661 aatggaggaa ttcttgagca taatatagac aaggtaaaag cctgtaaaaa ctgcgtaaac
     3721 tgtaactatc gatcccagcg ccacgatcgg aaagctcatt gctctgcctc catcgtgcgc
     3781 ccctccagca atattgaaat tgatcctacc tgtccttctc aagctttctc gttgcgaaat
     3841 ccgccgcctt attcagcaca ttataattct caccaaccat ggggcaataa gccatttcgg
     3901 tcctggccat ggaaaaaaca tcaataccac atcttattcc aagagaaata acattgataa
     3961 atgccccggc gccctctcct actgcctgac ctccaaacac tttgcctgtt cttgcatctg
     4021 cgattatctt tactgttata tcttttgcgc cgggataata ttccggctta ctttttccat
     4081 gcgctttgcc cgaaactatc ttatatcctg cagattctgc aacgaattcg ttaaaaccgg
     4141 tggctgcaat ctcaacattt ccaaggacag taacaaaagt gccaagcgct ccttcatatg
     4201 tatcataacc acctgcagcg ttagtgccag ctaccattcc ctgtctgtaa gccggtgatg
     4261 ataactgcat cattgcaggt ctgtgattga tgaggctcac ggtctggatg cagtcaccta
     4321 ccgcataaat ccctttgata tttgtttcca tcccagcatt cgttttaatg ccccagttct
     4381 catgttctat tcctgcattt ttggcgattt caaagttggc cctgacaccg gatgccagga
     4441 ttaccatatc agtttcaatt atttctccgc ctactaaaac cgaattcaca ggaggaccat
     4501 ttatgctctt tacgctttta tcaaatatca attttatacc gcctgcctca agatgtcgtt
     4561 ccaccagctt tgccatatcc ctgtcaagtg cgcggggcat ggcgaaggaa agcatctcga
     4621 ctaccacaac cccaagacct cttgttcgca aagcaacggc aatctcaagg ccaatgggtc
     4681 ctgctcctac aactgccact ttttttacct tcccggcacg gtctgaaatt gtattggcgc
     4741 tttcaatatc atgaagcgca taaacgccat tacctaaatt ctcttttgcg ccttttatgg
     4801 gtggaatgaa tggctctgct ccctgtgcga taattaagaa atcataggga atagacagaa
     4861 tctcaccggt tcccaggttc ttcgtcttga catttttcgc aatcggatca attgacattg
     4921 cttcatgcat caggagtttt gtagattgtt tatcttccgg gagcgagaaa ctgagtttgt
     4981 caagattgat tataccttct attgcatagg gcatgccgca aggtgaaaac atttgatatg
     5041 gtcttttttc tattatcgtt atgaaagcat ccgggtcagt ccttcggata gcgagaaaag
     5101 ctgcaaatcc tccgcttccc aggccaatta ttactatatt taccatcttt gatattgggt
     5161 atcagatgat aatatagttt gccaagtcaa aagctttatt atgttttaaa cacaccaaca
     5221 taaaattcaa tatatttacc atctttcaaa agacttattt ataggaatgt aattatcatt
     5281 aatattatgg cttgtcaggg tttatatata tgaatatcta aatagtatca taagcacaat
     5341 aattatgagc gactatacaa cgggtattaa ggaactggat gatgcgattg gaggcataaa
     5401 aaaagggtca aatattatgc ttatcggacc tcccatgagt gggaaagagg ccatcctgaa
     5461 ccacattttg taccacggcg ccgtagaaaa tggaaatgct gttattactg taaccacacg
     5521 cgaatcagcg gttcatattc ttgattggtt taaagaacac aagttgaacc ttcccatgga
     5581 aaggatcggg ataattgact gcgttacaaa aactctcggc gggcctgtgg ttgaaaatga
     5641 aagtgttaaa attgcaagta gtcctgttga tctgacagga atcggggtta agatcagcca
     5701 gttttttgag gaattcttta tgaaaaagaa tacacagaaa atacagttgc atattaattc
     5761 actatcaaca atattgatgt attctaacat acagactgtt ttcaggttcc ttcatgtttt
     5821 taccgggcgt attaaagcag ccagcgctct gggaatttat gtaatagaga gcggcatgca
     5881 tgacgaacag gcagttgcta ctttaaaaca attgtttgac ggtatgatcg aaattaaatc
     5941 cgaggaggat aaaaacttta tacgtgtggt gggcatatct caaaaaccaa ctccctggtt
     6001 tgaatatgag gtccagggac ataatgtaaa gatcataagg ggcaattagg atggtggaga
     6061 caggtatccc caagcttgac gaatttcttg ggggaggcat cccaaaaggc aagagtcttg
     6121 ttttttacag ccagccaggt gtggaaggtg agatattcgg attgcagact gtccaccaaa
     6181 cacttaagat gggggaaaca tgcgtatttg ttgtatcgag cactatacct gatatcgtaa
     6241 aagggcagtt caaagagttt ggctgggata ttagcctgtt caaagataaa ttattctttg
     6301 tagattccta taatcctttg ataggcgctc catccaacga aaaatacctt atctcaaatc
     6361 cagataaaat agaagatttc aataagacaa tagttgatat cattaaagaa ttaccgccaa
     6421 gcactatcgt ttttggttca ctttccacaa ttatggacct ttgcggtgaa aaagaaacca
     6481 ttgaagctgt caggatgtgg aataaaatgg ccatgttgta taatcatgtc attgtttata
     6541 atttcacagc ctggccttat tctgatgaaa catcagattt gataaaagga gaccttttca
     6601 attctgttat atctattggc ggaatagctg aacgtgttat ctttggccag tatttcggaa
     6661 ttttgaaatc agactgggcg cagataacca ggaaatccat gcttttcagg gttttaaggc
     6721 cgggtgggat aaagatattc ataccgaaaa tacttgtcac aggtcctttt gatgccggga
     6781 agagcacatt tgtccatgcg ctttctaccc gggcgatatc ggttgatagg ctcggtacaa
     6841 ctattgccat ggaccatggt catgttgatt ataagggttt tagcgctgat atattcggta
     6901 caccgggcca ggaacggttt gacccgatta taaaattact cagcggcgaa tccatgggtg
     6961 tttttcttgt tgtggattca acaaaccctt ctgattttat tcgcgcaaag cacatgcttg
     7021 atattacaaa gtcctatggc ctgccgtatg ttgtggtcgc aaataaacag gatttaccgg
     7081 gagctttaac tcccgaagaa ataaaggaac aattccatct gccagaggac gtccctatcg
     7141 tacctgcag
//
LOCUS       DATGBN010000006         4414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_427606, whole genome shotgun sequence.
ACCESSION   DATGBN010000006 DATGBN010000000
VERSION     DATGBN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427606"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VKL21_00120"
     CDS             <1..707
                     /locus_tag="VKL21_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193514.1"
                     /note="catalyzes the formation of
                     tetrahydropteroyl-L-glutamate and methionine from
                     L-homocysteine and
                     5-methyltetrahydropteroyltri-L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methonine synthase"
                     /protein_id="HMB44203.1"
                     /translation="RLCVTGPIELYINQFGSTAYKDVLNAFATSIDRFISNSIENAKQ
                     FRIAVISIDEPSIGINPNIMFNDGDMIEALTIATKTAGSNKIDTEIHLHSPLHYKLIC
                     MVPYISIIGVESAANPSYLDLIDKKDLEDSEKYLRVGVARTDIFGMAAALNEKYNTNV
                     WKEPAKLDEITTQMETRDIIAKRLGKAYSIFGRRIKYAGPDCGLGSWPSQELASNLLR
                     NTCAGIKEFRKNQETG"
     gene            740..1849
                     /locus_tag="VKL21_00125"
     CDS             740..1849
                     /locus_tag="VKL21_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB44204.1"
                     /translation="MRAKQRVNNKKGNKYEPGIKCTGTKTVTVKPAGYPLKGMFHEYP
                     EPCELDVFEFYAREQWNGYVIKKGDFLFDRRMFPDFAFKVIEVEPDNSEIGKNTKFLV
                     EDYESDFFVPDMKKEVTFKNIIGQEKAKQKCKLIESFLENPEKFGTWVPKNILFHGPA
                     GTGKTMMAKALATATDVTFLPVRATKLIGEYVGDGARQIHQLYEKADEMAPCIIFIDE
                     FDAIALNRNYQEIRGDVSEIVNALLTELDGIDEHEGICTIAATNMLHTLDSAIRSRFE
                     EEIEFTLPTLEERCEILKFYVRTFPKKLAKDVDIDLLAKLTPGFSGRDLVEKLLKNAL
                     HKAILAGGKVNRAHFEAALSESLCKRSEPPRGMFA"
     gene            1849..2328
                     /locus_tag="VKL21_00130"
     CDS             1849..2328
                     /locus_tag="VKL21_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44205.1"
                     /translation="MVIKSVSSLMGNLKQSYDKSKDKSGWHVLSGINREYNDTFIAGD
                     KKLWQIKSEEVRPGESVAVGMKVSKFDEDLEKIMKNGSPVPFGTVTPIDKKISVIMAG
                     MQTFSSDSSNQLCKEHLSSKQVELEVKLKQQVDLMMDDRSFRRKYLEHKDRLRRAYL"
     gene            2372..2444
                     /locus_tag="VKL21_00135"
     tRNA            2372..2444
                     /locus_tag="VKL21_00135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2406..2408,aa:Gln,seq:ttg)
     gene            3274..>4414
                     /locus_tag="VKL21_00140"
     CDS             3274..>4414
                     /locus_tag="VKL21_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMB44206.1"
                     /translation="MGNKEIVIGIGILLILLALAGTGSAHGVYGTIGANCAFCHAGYP
                     TPLTANGIFFKDNHKFDGLTEPLTSATSCTTCHTNLNTVFLPLTQAGQNYSNSHRYNN
                     TTLAATRLAPPACYNCHVDANNNNFNLLTGTPTYLTSTTCEACHKAKYDKWSNTLHAV
                     MLTPKVKAQAMNLPIPPVGWANISYVIVTKFEFAYLNTTGYFPAQNDAYETETKEFVN
                     SSHAGGAYGTCGRCHTTNWNASARDSALPGFYGTFSEPGIACERCHKPAGNGHQVVVN
                     YSGDQCRECHTGGNHGTGWENGEHAPPPYENGSNCMFCHSSFDQYKNQNVTIANATGV
                     SCGVCHNIHDMTDNKYAATFSEGIFNATTWSEVADSKLSFFNATAS"
BASE COUNT         1407 a          867 c          984 g         1156 t
ORIGIN      
        1 tcaggctttg cgttacggga cctatagaat tgtacatcaa tcagtttggt tcaactgctt
       61 ataaagatgt cctgaatgca tttgcgacaa gcattgacag gtttatcagt aattccattg
      121 aaaatgcaaa acaattccga attgcagtga tctcgataga cgaaccaagc atagggataa
      181 acccgaacat catgttcaat gatggagata tgatagaggc tctcaccatc gccacaaaaa
      241 ccgcgggttc aaataagatc gatactgaaa tacatctaca ttcaccgctt cattataaac
      301 tcatttgcat ggtgccttat ataagtataa ttggcgttga atctgctgcc aatccctcat
      361 atcttgacct aattgataag aaggatcttg aagattctga aaaatattta agggtcggtg
      421 tagcacgtac tgatattttc gggatggctg cggcgctgaa tgaaaaatac aacacgaatg
      481 tctggaaaga accggcaaag ctggacgaaa tcacaacgca gatggagacg cgcgacatca
      541 tagcaaaaag gcttggaaaa gcatactcga tatttggcag gaggataaaa tatgcaggac
      601 ctgactgcgg gcttggctca tggccatccc aggaattggc atccaacctt ttgagaaaca
      661 catgcgcagg tataaaagaa ttccggaaga accaagaaac agggtaattc tttaatagat
      721 gagggacaat aattgaatta tgcgagcaaa acaacgggta aataacaaaa aaggcaataa
      781 atacgaaccc ggcatcaaat gcacgggaac aaaaacggta acagtaaaac ctgccggtta
      841 ccccttgaaa ggaatgttcc atgaatatcc cgaaccctgc gaactcgatg tttttgagtt
      901 ctatgcaagg gagcagtgga atggatacgt aataaaaaaa ggagacttcc tgtttgacag
      961 gaggatgttc ccggattttg cttttaaggt cattgaagtt gaacctgata actcagagat
     1021 cgggaaaaac acaaaattct tagttgaaga ttatgagtct gatttctttg ttcctgatat
     1081 gaaaaaagaa gttactttca agaatatcat cgggcaggag aaagcaaaac agaaatgcaa
     1141 gcttattgag agttttcttg aaaatcccga gaaattcggg acatgggtgc ccaagaatat
     1201 tttgttccac gggcctgcgg gaacgggaaa aaccatgatg gcaaaggcgc ttgcaactgc
     1261 aacggatgtt actttcctgc ctgtaagggc cacaaaactc ataggagaat atgtcggcga
     1321 tggggccaga caaattcatc agttatatga aaaagctgac gagatggcgc cttgtattat
     1381 tttcattgat gagtttgatg caatcgccct taaccggaat tatcaggaga tccgcggtga
     1441 tgtgtcggaa atagtcaatg ctcttctcac cgaattagat ggaatcgacg agcatgaagg
     1501 gatatgcacc attgctgcaa caaatatgct ccatactctt gattccgcga tacggagccg
     1561 ttttgaagaa gaaatagaat ttaccctgcc aacccttgag gaaagatgtg aaatattgaa
     1621 gttttatgtc aggactttcc cgaagaaact cgcaaaagat gtggacattg atctgcttgc
     1681 aaaactcaca ccgggtttct caggcaggga tcttgttgaa aagttgttaa agaacgcatt
     1741 gcataaagcc atactggccg gcggcaaagt aaatagggca cattttgagg cagcgctttc
     1801 cgaatcgctt tgcaagaggt ccgaaccccc cagaggtatg ttcgcataat ggtaataaaa
     1861 agcgtgagtt ccttgatggg gaacctgaaa caatcctacg ataagagcaa agataaatcc
     1921 ggatggcacg tcttaagcgg tatcaaccgg gaatataatg ataccttcat cgcaggtgat
     1981 aaaaagttat ggcagattaa atcggaagag gtaagaccgg gagaatctgt tgctgttggt
     2041 atgaaagtgt cgaagtttga tgaagatctt gaaaaaataa tgaagaatgg atcccccgta
     2101 cctttcggga ctgttacccc tattgataag aaaatttcgg taataatggc agggatgcag
     2161 actttttctt cagattcatc caaccaattg tgtaaggaac atctttcgag taaacaggtg
     2221 gaacttgagg ttaaacttaa acagcaggtt gatctgatga tggatgacag gtctttcaga
     2281 aggaaatatt tggaacataa agacaggctc agaagggcat acttataaca ctcccataaa
     2341 gaataagtag tttagttacg attaagcctt cagtcccgta gggtagtggt caatccttct
     2401 ggcctttgga gccggagacg acggttcgaa tccgcccggg actactttct tcaaattaat
     2461 agtccgcaca tgttttttgg cagagtgcgc atgacttttt attcaaatcc ttgcatggct
     2521 actttcccca gttttatagt ccttgcattt ttgcacttcc agtttaacta gcaattcaag
     2581 cactccaaca agcacggcgc aggatttcac ccagacccat gaaagaagaa gaaagctcca
     2641 atttttgaca tgatgtgcga ttcatgccat tatggggtaa tgtgatgtct ctcaagcagc
     2701 ccaacaacga attaacaggt tttatccgta ttgttaattt ttttctcaca ggaatcatgg
     2761 caaagcgtga gtattaatag agtaaaccaa tagaataaat agaaaatgta gaggatttca
     2821 agagttcttg gatagtttgt tgataggata ggttggaagt gaatcttgta aaagcgttat
     2881 gtatcctcta cgcattcaat aattgggata tgcttaaata gcatttttcc aagaaactcc
     2941 atccattgaa ccgattgctg tgaaaccggt tgagaaatta gcaccagcag actatacact
     3001 tcccgatata tcgtgttacg aatactattc ccgcctgcaa tcactccgga tttatagcgc
     3061 tgagcagtca ggtcgctttg gtaacaccct tataaaaata aaaaagttac cacaagactt
     3121 attaccatct aaatactata ttccgatttg gatatgggag taatgtttgt ccataaagat
     3181 gattttcatc tttcattatg ccatttataa aaattgtgat ttggcttggt cagacatgaa
     3241 attatataaa tgagatatgg gaggaaatgg attatgggaa ataaggaaat tgttatcgga
     3301 ataggaatat tattaatact gctggcgctg gcaggaactg gaagcgcgca tggtgtttat
     3361 ggaactatcg gagcgaattg cgccttttgc cacgctggat atccaacacc attgacggct
     3421 aatggtattt tctttaaaga taatcacaaa tttgatggct tgacagagcc gctcacatct
     3481 gctacaagct gtactacatg ccatacgaac ttgaatacgg ttttccttcc cctgacccaa
     3541 gctggtcaga attacagcaa cagccacagg tacaacaata ccacgctggc agctacgagg
     3601 ctagcaccgc cagcctgtta taattgccat gtggatgcca ataataacaa ttttaattta
     3661 cttacaggca ctccaaccta cctgacatcc acaacatgcg aagcttgcca caaggcgaag
     3721 tacgataaat ggagtaacac attgcatgct gttatgttaa cgccaaaagt taaagctcag
     3781 gcaatgaatc ttccaatacc gccggttggc tgggcgaaca tatcctacgt aattgtaacc
     3841 aaattcgaat ttgcatatct caatacaact ggatatttcc ctgctcagaa tgatgcatat
     3901 gaaactgaaa ctaaggagtt tgtaaatagt agtcacgctg gcggagcata cggcacctgt
     3961 ggaagatgcc atactactaa ctggaatgca agcgccaggg atagcgcatt accaggcttt
     4021 tatggaacct ttagcgagcc aggaattgcc tgtgagagat gtcataaacc agcaggcaac
     4081 gggcatcagg tagttgttaa ttattccggg gatcaatgcc gtgaatgcca taccggtggt
     4141 aatcatggaa cgggctggga aaacggtgaa catgcaccac ctccgtatga aaacggcagc
     4201 aattgcatgt tctgtcattc gtcgtttgat caatataaaa accagaatgt gactatagcc
     4261 aacgcgacag gcgtctcgtg tggagtatgc cacaatatcc acgatatgac agataataaa
     4321 tatgcagcaa cattctcaga aggtattttc aatgcaacaa catggtctga ggtagcagat
     4381 tcgaaattaa gtttcttcaa tgccactgct tcta
//
LOCUS       DATGBN010000007         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_505367, whole genome shotgun sequence.
ACCESSION   DATGBN010000007 DATGBN010000000
VERSION     DATGBN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505367"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..421)
                     /locus_tag="VKL21_00145"
     CDS             complement(62..421)
                     /locus_tag="VKL21_00145"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HMB44207.1"
                     /translation="MIKTKEKQIFSARSILQSLGIWTSEYYKFIIMFGILFTILQISD
                     LTITHQALKNSEIKELNPLYSQIWFVPFKLTMVFLIMAVMYRIPAPNQIFAKKAMIGM
                     IFMYVFINMNNLYLVLK"
     gene            577..1140
                     /locus_tag="VKL21_00150"
     CDS             577..1140
                     /locus_tag="VKL21_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HMB44208.1"
                     /translation="MKNEIIIGISGASGIQYGIRLLEAIKQIKNYETHLVISGSAKKL
                     IEIETDLSVSDVEKLGDNVYDDYDFTAPIASGSHRSRGMIIAPCSMKTLASIACGMSD
                     TLISRAADCCLKEKRPLILMARETPLNLIHVENMKKAMEAGASILPACPAFYPKPKTI
                     DDIIDFMAGRALDLLDIEHNLYKRWRE"
     gene            1137..>1534
                     /locus_tag="VKL21_00155"
     CDS             1137..>1534
                     /locus_tag="VKL21_00155"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HMB44209.1"
                     /translation="MSFRGFLEILKEKGKLTEINEPLSPVYEVSSMVGNEPTLFTDVN
                     NSKVVMNILASRDLLAEALGVAPEKIIEHLSSCPPEGEVKLVKDSPTKEVVEKPDLYK
                     LPILTHFEGDGAPYITAGVVVSQYQGLMNAS"
BASE COUNT          503 a          274 c          305 g          452 t
ORIGIN      
        1 gtaaaagaga aaaaaggaga gctttaagaa aataaagaat attatcgaat ttgccacagg
       61 ctcatttcaa aacaagatac agattattca tatttatgaa cacatacata aatatcattc
      121 cgatcattgc tttttttgca aatatttggt ttggtgccgg aatgcgatac ataactgcca
      181 taataagaaa taccatcgtc aatttgaatg gaacaaacca tatctggcta taaaggggat
      241 taagttcctt aatttcggaa ttcttgagtg cctggtgagt gatagtaagg tctgaaatct
      301 gcaatatagt gaataatatt ccaaacatta ttataaattt ataatattcc gatgtccaga
      361 tgcccagtga ttgcaatatt gaccttgcgc taaaaatctg tttttctttg gtttttatca
      421 tttcacacac caaagtaaaa taagatcttt atctacttaa atatatctat ttatttagat
      481 aattataata gttattatta tcatataaag ttttaatttt gatctaatac ttttttttca
      541 gtaaactatt caaataaagc aaaccaagta tagcctatga agaatgagat aattatcggg
      601 ataagcggtg cctcaggtat ccagtatggg ataagattac ttgaagccat aaaacaaata
      661 aaaaattatg aaactcatct tgtcatttcc ggatctgcaa aaaaactcat tgagatagag
      721 accgatcttt cggtcagtga tgttgagaaa ttgggtgata acgtctatga cgattatgat
      781 tttacagctc cgatagcaag cggttcgcac aggtcaagag gcatgatcat tgcgccatgc
      841 agcatgaaaa ccctggcatc gatcgcatgc gggatgtcgg atacactgat atcaagagcc
      901 gccgactgct gcctgaaaga aaagcgcccc ctaatattga tggcgaggga aacgccactt
      961 aatctaattc atgtggagaa catgaaaaaa gcaatggagg caggcgcatc aattcttcct
     1021 gcatgcccgg cgttttatcc gaaaccaaaa accatcgatg atattattga ttttatggct
     1081 ggaagggctc ttgaccttct tgatatcgag cataatttgt ataagcggtg gcgtgaatga
     1141 gtttcagggg atttctggaa atactaaagg agaaaggcaa actaacagaa atcaatgagc
     1201 cgctatcgcc ggtttatgaa gtttcttcga tggtgggaaa tgaacccact ctttttacag
     1261 atgtgaataa ctccaaagtt gtcatgaaca tccttgcgtc gagagattta ctcgcagaag
     1321 ctcttggcgt tgcgcctgaa aaaataatag agcatctttc atcatgccct cctgagggtg
     1381 aagttaaact ggtcaaggat tcacccacaa aagaagtagt ggaaaaacct gacctgtata
     1441 aactccccat tctcactcat tttgaaggag acggcgcccc gtatataact gctggtgttg
     1501 ttgtttcgca atatcagggt ttgatgaatg cttc
//
LOCUS       DATGBN010000008         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_233546, whole genome shotgun sequence.
ACCESSION   DATGBN010000008 DATGBN010000000
VERSION     DATGBN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233546"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..537
                     /locus_tag="VKL21_00160"
     CDS             358..537
                     /locus_tag="VKL21_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HMB44210.1"
                     /translation="MVSIKDMSLSKKLIGGFALVVLLLGIVGYVGYNGIRKITNELHN
                     INTISSEAATASVRI"
     gene            553..2070
                     /locus_tag="VKL21_00165"
     CDS             553..2070
                     /locus_tag="VKL21_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HMB44211.1"
                     /translation="MKIIKILDKANELTINRFNIGIKIAYSFIVVDMLLSIVVFIGLY
                     GESISAIIDPRQAIILCFLFAIFSSILMCIGLTRSIMKPLDNFINAADRVAEGDLTTD
                     IDVSSNDELGQLAAYFRKMTSNLRNITGKVQEVSIKVANTAQELSASSEEMKTSSDQI
                     SSTTQDIATGVSSQASKMSEISRAMKEMSESVQQVATNSQKAAESASSASTTAQKVGK
                     MSDDMAKKMTDIQSTVDNSATVIKQLDGKSQQIGEIISVITNIADQTNLLALNAAIEA
                     ARAGEHGRGFAVVADEVRKLAEESRNAANQITGLIKDIQQGTKQAVTSMEQGTKTVGE
                     GGKNIQQTVTAIDEIVKAAADVATMVQEIAAAAQEQSASVEEVTASVEDVSAISEESA
                     AGAQETSATAEEQSASMDQLVNAAQDMARLSNELQAEVAKFNIGEAVSTSKQAYEPSK
                     IEHRPVPEHKTAKHKPQGGQKKVNEHSTSGGMTGKDERAQVRNNLTGSSLEDLTK"
     gene            2080..2598
                     /locus_tag="VKL21_00170"
     CDS             2080..2598
                     /locus_tag="VKL21_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMB44212.1"
                     /translation="MAEKQLQDAKAKSGDELQLVVFNIGTEEFGVEIMNVQEIIRMTN
                     ITKIPQASGYVKGIINLRGRIIVVINLNVIMGMESKEQNENTRIIVADIGDTVMGFIV
                     DSVSEVIRLPESSVEPAPAVIANKIGTEYVRGVGKMEDRLLILLDLDKILSANELHNV
                     NTIASEALAATT"
     gene            complement(2813..3382)
                     /locus_tag="VKL21_00175"
     CDS             complement(2813..3382)
                     /locus_tag="VKL21_00175"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HMB44213.1"
                     /translation="MMQKRAIITVSGDVQGVGYRDIVNKIARKMKITGFVEYIKPYDV
                     KIICEGSEENISFFIDKLNIKKFPVDVEGIKFDLNEPSGEFEIFEVKRGDIADELGER
                     LDVARLEMTKMVEKQDGALSRQDSMLEKQNSMLDLQRKTIDTIKLEGEKTRDTISIHV
                     SNDLADLRQEIIHMKSNLSKVMDKIGISE"
     gene            3799..>4236
                     /locus_tag="VKL21_00180"
     CDS             3799..>4236
                     /locus_tag="VKL21_00180"
                     /inference="COORDINATES: protein motif:HMM:NF028510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HMB44214.1"
                     /translation="MKIKDISIKHKIIIFALILSIIPVTIIGLYAYNEASNSLNKEVQ
                     NKLEEQVSLEKDNIETTFYLAQNNVNNSLGVAKAQFYSRGKPVIKDGKMQLGENYVLN
                     GNFEIVDTVKNMVGGTATVFQVRGDEAVRVSTNVVNTDGTRAVG"
BASE COUNT         1412 a          799 c          862 g         1163 t
ORIGIN      
        1 attcatggta tagctttctt gtcgtactaa gaatacttat acgcagttct gatgaggact
       61 ttaccagaaa acaatatcag tgtgaaatat atgtgcaata gatttaagac tatgttcaat
      121 ccaagaaaga ataagataga ctccccactt tctagatata tctttattgg gagcctatct
      181 tcaattccat gacatcagtt aagccattat ttatcgtttt gtataaattg atgaacataa
      241 attaattttt cacacttttt tcacaaatca gtttatgaat ctgtggtttt cttagaatta
      301 catgaaagag cgaattttgc tcatcagaaa aataaattat gctaatggag aacaaaaatg
      361 gtaagtataa aagatatgtc tttaagcaag aagttgatcg ggggttttgc tctcgtggta
      421 ttacttctcg gtattgtagg gtatgtaggc tacaatggta ttcgtaaaat aaccaatgaa
      481 cttcataata taaacaccat atcctccgaa gctgcaacag cgtccgtcag aatataattc
      541 atggagaata tcatgaaaat aataaaaatt ttggacaagg caaatgaatt gaccattaac
      601 aggttcaata ttggcataaa aatcgcttat agcttcattg tggttgatat gctgttatcg
      661 atcgtagtat tcataggatt atacggtgag agtatcagcg ctataattga cccaagacag
      721 gcaatcatcc tttgtttctt atttgctatt ttctcatcca tactcatgtg tatcgggttg
      781 acccgttcta taatgaagcc tttggataat tttattaatg cagctgacag ggtagctgag
      841 ggagatttaa caacagatat agatgtctct tcaaatgacg aactaggaca acttgccgct
      901 tatttcagga aaatgacttc aaacctcagg aacataactg gcaaagtcca ggaagtctca
      961 ataaaagttg cgaatacagc ccaggaactc tcagcatcaa gcgaggaaat gaaaacttca
     1021 tcagaccaga tctcaagcac aacgcaggac attgcaacag gagtaagctc acaggcgtcc
     1081 aagatgtctg agatcagccg tgctatgaag gaaatgtcag aaagcgtcca gcaggtcgcc
     1141 acgaactccc agaaagctgc tgaaagcgca agttcagcca gcacgacagc tcagaaagtg
     1201 ggtaagatgt cagacgatat ggcgaaaaag atgactgata tacagtcaac tgtggataat
     1261 tctgcaacgg taataaaaca gcttgacggc aaatcacagc aaatcggcga aattatcagt
     1321 gtcatcacaa atattgcaga ccaaacaaac ctccttgcgc ttaatgctgc catagaagcg
     1381 gcacgtgcag gagagcacgg aagaggtttt gctgtagttg ctgatgaagt aaggaaactt
     1441 gccgaagagt caagaaatgc tgccaaccag atcaccgggt tgataaaaga tatccagcag
     1501 ggaacaaaac aggctgtaac gagtatggaa cagggcacaa agacagtagg tgaaggagga
     1561 aagaatatac aacagactgt gactgccata gatgaaatag tcaaagctgc agcagatgta
     1621 gccacaatgg tacaggaaat agcggcagcg gcacaagaac aatccgcatc ggtcgaagaa
     1681 gtaacagcat ctgttgaaga tgtatcagca ataagcgaag aatcggcagc aggcgcacag
     1741 gaaacatcag caactgcaga agagcagtct gcttcaatgg accagcttgt aaacgcagcc
     1801 caggacatgg caagattgtc aaatgaattg caggccgaag ttgctaaatt caatatcggt
     1861 gaagcggttt caacttcaaa acaggcatat gaaccatcaa agatcgagca tagacctgtt
     1921 cccgaacaca agacagcaaa acacaaacct cagggcggtc agaagaaagt gaatgaacat
     1981 agtacttcag gcgggatgac aggtaaagac gaaagagccc aggtcaggaa taatctaacg
     2041 ggttcaagcc ttgaagatct gacaaaataa gaggacgaaa tggctgaaaa acaactccaa
     2101 gacgcaaaag caaaatcagg cgatgagctt cagctcgtcg ttttcaatat tggcactgaa
     2161 gagttcggtg ttgagataat gaacgtgcag gagatcatcc gcatgaccaa tatcacaaag
     2221 ataccgcagg catcaggcta tgtgaaggga ataatcaatc ttcgcggcag gataatcgtg
     2281 gtcatcaatc ttaatgtgat catgggcatg gaaagcaaag aacagaatga gaatacccgc
     2341 atcattgtcg cggatatcgg cgataccgtt atgggcttca ttgttgattc cgtaagcgaa
     2401 gtaatacgcc ttcctgaaag cagcgttgag ccggcgccgg ccgtgatcgc caataagatc
     2461 ggcacggaat atgtgcgcgg tgtgggcaag atggaagaca ggttgcttat cctgctcgat
     2521 cttgataaga tactcagcgc caacgaactt cataatgtaa atacaatagc ttccgaagct
     2581 ctagcagcga ccacataagc tgcttttatt tctttttttc atataaatga gactcatttg
     2641 acattttcag aaacctatac ccaaatcctt ttatattgca gaatctaatt taattgatat
     2701 aattatggct gtagttaccc ttgaagaaat tcatgaagat ctgctttcaa ttaagaatga
     2761 tatgactaaa attaagttat attttgaaga agatgatctt ctgctttatt tattattctg
     2821 aaattcctat tttatccata acttttgaga gattggattt catatgaatt atttcctgtc
     2881 gaagatcagc aagatcattt gaaacatgga tagatatagt gtctcttgtt ttctctcctt
     2941 caagtttaat agtatctatt gtttttctct gcaaatccag catgctgttc tgtttttcca
     3001 gcatgctgtc ctgtcttgac agcgcaccat cctgtttttc caccatcttt gtcatttcaa
     3061 gtctggcaac atcaagtctt tctccaagtt catcagcgat atcgcccctt ttgacctcaa
     3121 aaatttcaaa ttctcctgag ggttcgttta aatcgaattt tattccttca acatcaactg
     3181 gaaatttttt aatattcaac ttatctataa aaaaggaaat attttcttct gacccctcac
     3241 aaataatctt tacatcatac ggttttatat actcaacaaa tccagtaatt ttcatttttc
     3301 gtgcaatctt gttgactata tccctgtatc caacaccctg aacatcaccg gatactgtta
     3361 tgatcgctct tttttgcatc attgattcat ggattggagg atataaatag attgttgttt
     3421 ttaatatcca caactttttc atttattccg gagaaaatgt tcatcgcaag tagaatcaac
     3481 cacagagtcc acagagagcg cagagatttg acgcttaata gcttttaata tcatattgct
     3541 aactatgaga tagcaatatg ttagcaagaa acatatccgc agaagcgtca ggtgctacag
     3601 cataagatat ggcccttttc ttttcatttc acacgttttt cacaaatccg tttatattcc
     3661 cgatatcgta tatctaacta aattataaaa aacggatcgg gtattcatgc tgtcttattg
     3721 gaagagcggc aaagataatt gcattgtctg tcctaatttg caggaacatc agaaaaaaaa
     3781 tcaataggag aaacagagat gaaaataaaa gatattagta taaaacacaa aattataatt
     3841 tttgcattaa tactttcaat aattcccgtg acaattatag gtttatatgc atataatgaa
     3901 gcaagtaact cgttgaataa agaagtccag aataaactgg aagagcaggt aagtcttgaa
     3961 aaagataaca tagaaaccac attttatctt gcgcagaata atgtcaataa ttcactcggg
     4021 gttgcaaaag cccagttcta ttccagggga aaacccgtta tcaaggatgg caagatgcag
     4081 cttggcgaaa attatgtact gaatgggaac tttgagatcg tggacacagt taagaatatg
     4141 gtcgggggta cagcaacggt attccaggtg agaggcgatg aagcggtcag ggtatccaca
     4201 aatgttgtaa atacggacgg tactcgcgca gtagga
//
LOCUS       DATGBN010000009         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_155838, whole genome shotgun sequence.
ACCESSION   DATGBN010000009 DATGBN010000000
VERSION     DATGBN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155838"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VKL21_00185"
     CDS             <1..573
                     /locus_tag="VKL21_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44215.1"
                     /translation="DAGTFLSRGFKLPLAVSMRAYHVDENGNIYQPQKLQAWNESVTL
                     LINQEPDYLDAFRPDPSNPRLYTLKVRNNNLLGVTGLHVLPTLDPWIATVNMWSIDVE
                     GEFVKFEVQDVDNEVHPDPIFGHEVQVYVREDVPDVYDSVTGLSIGSNMPIKFYFTTG
                     TFLIVPPGKIQGVGDKDGEIIEESPGYEVK"
     gene            573..1178
                     /locus_tag="VKL21_00190"
     CDS             573..1178
                     /locus_tag="VKL21_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcinarray family MAST domain-containing
                     protein"
                     /protein_id="HMB44216.1"
                     /translation="MKIRISILFYFMVYFLIFQIASAEENKYGIVKAWFNGENATVET
                     INGIQLKIGEPAEVKVEVISKINGHISLKLKEPGITTAYAVLSGPSKIDEKISNLNIE
                     SDWLETYTWSLIPNGAWKNGNAPINIIVQFHDLKTKNDEIIQFTIANPYILDEQYSGA
                     VPTPETTPSPAGTEAKAVPFLSVIFTVSTLIMAWSWRRGKQ"
     gene            1449..2474
                     /locus_tag="VKL21_00195"
     CDS             1449..2474
                     /locus_tag="VKL21_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMB44217.1"
                     /translation="MKRKSIVIILITVLISALLSGCVDKTETKDQSTPAGAVTSTPVA
                     TAASTAGTQELEGTISLSGAWALYPMAVRWGEEFTKLHPKVKVDVQAGGAGKGMADAI
                     GGLVDIGMVSRDIDPSEIEKGAYPIGVTKDAVVATINAKNPVLNDLLGKGVKSKTFVN
                     IFINGSVKTWGEVVGNPAIKDEIHVYTRSDAAGAPEIWAKYLGKKQENLNGTGVYGDP
                     GVLAAVQKDPLGIGYNNYNYALDMKTGLPIPGVQIIPFDVNENGKVDPDEDISTKEKT
                     IASIKKGVFPHPPARVEFFVTKGKPTGLTAEFIKWTLTDGQKFLDEVGYIELSKEILN
                     EELKKVD"
     gene            2528..>2917
                     /locus_tag="VKL21_00200"
     CDS             2528..>2917
                     /locus_tag="VKL21_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44218.1"
                     /translation="MFSRRLKDIIASRLMLAAAVLSSVLLPIIAIALYMRAKPILDAK
                     SMGDLLLGSTWMPFQGDFGFYPFIMGTLYVTLLAMVIAVPLSILCAIYLAEYAHENVR
                     TKVLPVIDLLAGIPPVVYGVWGVLAVVP"
BASE COUNT          896 a          560 c          686 g          775 t
ORIGIN      
        1 gatgcaggaa ccttcctgtc ccgcgggttc aaactgcctc ttgctgtgag tatgagggca
       61 tatcatgtgg atgagaatgg aaatatttat cagccccaga agctacaggc atggaatgag
      121 agtgtgacgc tgttgatcaa ccaggagccg gattatcttg acgcttttag accggatcct
      181 tctaatccac gattgtatac gctcaaagta aggaataaca atttgcttgg cgtaacagga
      241 cttcatgtgc ttccgaccct ggatccgtgg atagcaaccg ttaatatgtg gtcgattgat
      301 gttgaaggtg aatttgtgaa atttgaggtg caggacgttg ataatgaagt gcatcctgac
      361 ccgattttcg ggcatgaggt gcaggtgtat gtcagggaag atgttccaga tgtttatgat
      421 tctgtaacag gtttatccat tggaagcaac atgccaatca aattttattt tacaacaggg
      481 acttttttaa tagtccctcc tggcaaaata caaggtgtag gagataaaga tggagaaatt
      541 atcgaagagt ctccaggata tgaggtgaaa taatgaaaat tagaatatca atattatttt
      601 attttatggt ttattttttg atttttcaaa tagcatctgc agaagaaaat aaatatggta
      661 tcgttaaagc atggttcaat ggggaaaatg caacagtgga aactataaat ggcattcaat
      721 tgaagatagg agagccggca gaagtaaaag tagaagtaat atcaaaaatt aatgggcaca
      781 tatcactaaa actcaaagag cctgggatta ctactgctta tgctgtgttg agtgggccaa
      841 gtaaaataga tgaaaaaata tccaatttaa atatcgagtc tgattggctt gagacatata
      901 catggtcact tatcccgaat ggagcatgga aaaatggaaa tgcgcctata aacattatag
      961 ttcagtttca tgatctaaaa acaaaaaatg atgagataat ccaattcacc atcgccaatc
     1021 cctacatcct cgacgagcaa tattccggcg ccgtccccac gcctgaaacg acaccctccc
     1081 cggcgggaac cgaggcgaaa gcggtgccgt tcctttcggt aatattcacc gtcagcacac
     1141 tgatcatggc ctggagctgg agaagaggaa aacagtgaga tagcacacga atgatgctaa
     1201 tgcccggagt aatcatcgca acaattattg cctgtttctg tgcattctta tatgttcctg
     1261 cttatttgca gttgcttatt tatcttaagc aggtgaatca agaatgagtt taagaaggaa
     1321 tcgtctttgc tgttactgat ggcggcaaaa aaaatgaaaa gatttattga acatagcgtg
     1381 gaatttattc aaatccggga tttcctaaaa aaccaggaaa atatattaaa aaattcgagg
     1441 tggaatcaat gaagagaaaa tctatagtta ttattcttat tacagtttta ataagtgcat
     1501 tattatcagg ctgtgtagat aagacagaaa caaaagacca gtctacacct gccggcgctg
     1561 tcacttcaac acctgtagcc acggctgcat cgacagcggg aacacaggaa cttgaaggca
     1621 ctatatcctt atccggcgca tgggcgctct atccaatggc agtgcgctgg ggtgaagaat
     1681 tcacgaaact gcatccaaaa gtaaaggttg atgtacaggc aggcggcgcg ggaaaaggca
     1741 tggcagatgc cataggcggg cttgttgata ttggcatggt atcaagggat attgatcctt
     1801 cagagatcga gaaaggagca tacccgattg gcgtgacaaa ggacgctgtg gttgctacta
     1861 tcaatgcaaa gaaccctgtt cttaatgacc tccttggcaa aggtgtcaag agcaaaacat
     1921 tcgtgaatat ttttataaac ggatctgtga aaacctgggg cgaagtagta ggaaatcctg
     1981 caataaaaga tgaaattcat gtatatacaa gaagcgacgc cgctggagcg cctgagatat
     2041 gggctaaata tctgggaaaa aagcaagaaa acctgaacgg aacaggggtt tatggtgatc
     2101 ctggcgtcct tgctgctgtg caaaaggatc ccctgggcat aggatataac aactacaact
     2161 atgctcttga catgaagacc gggctgccta ttcctggtgt acagattatt cctttcgatg
     2221 tgaatgagaa cggaaaagtt gatcctgatg aggatataag cacgaaggag aagaccattg
     2281 cttcgatcaa gaaaggtgtt tttccgcatc cgcctgcaag agttgaattc ttcgtaacaa
     2341 aaggtaaacc cacagggctt acagcagagt ttataaaatg gactcttaca gacggccaga
     2401 agttccttga tgaagtagga tacattgaac tctctaaaga aatacttaac gaggaattga
     2461 aaaaggttga ttgaaaaggt tttaccttaa acctatattt tttattctaa tagaatatat
     2521 ataactcatg ttttccagaa gactaaagga tatcatcgca agcaggctca tgctggctgc
     2581 ggctgtctta tcaagcgtac ttttacccat aatagcgatc gccctttaca tgcgggcaaa
     2641 acccatcctt gatgcaaaat caatgggtga ccttctgctt ggcagcacat ggatgccctt
     2701 ccagggtgat ttcggttttt atccattcat catgggcacg ctctatgtaa ccctgttggc
     2761 gatggtgatc gcagtgcccc tatccatcct ctgcgcaata tatcttgccg aatatgcgca
     2821 cgagaatgta aggacaaagg ttctgccggt catagacctg cttgccggta tcccgccggt
     2881 cgtgtacggt gtatggggcg ttcttgctgt tgtcccg
//
LOCUS       DATGBN010000010         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_155885, whole genome shotgun sequence.
ACCESSION   DATGBN010000010 DATGBN010000000
VERSION     DATGBN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155885"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="lsrF"
                     /locus_tag="VKL21_00205"
     CDS             complement(<1..700)
                     /gene="lsrF"
                     /locus_tag="VKL21_00205"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HMB44219.1"
                     /translation="MNWGMKNRISRIIKPKSGRCVMLAVDHGYFQGPTTGLSNLGKTV
                     EPLLPYADALMITRGAIRNWIDPAVDIPIILRVSGGQSILKELSNETITTCIEDAIRI
                     NATAITCSVYVGGEFEKETIHNLSKLVDDGEKYGIPVLAVTAVGKDMVRDARYLALAS
                     RICIEIGAHIVKTYYTEDFGKVVEACRNVPVVIAGGKKLPEMDALKMAFSAISEGAVG
                     VDMGRNIFQSDSPVG"
     gene            complement(702..923)
                     /locus_tag="VKL21_00210"
     CDS             complement(702..923)
                     /locus_tag="VKL21_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44220.1"
                     /translation="MPIKGFEEYKRREYCNDIQCHVQMMMNKKTQDSEDYNDLREICQ
                     ENCLHTTYEFHHWLIEKGYLLVRPGKGGI"
     gene            complement(1034..2341)
                     /gene="thiC"
                     /locus_tag="VKL21_00215"
     CDS             complement(1034..2341)
                     /gene="thiC"
                     /locus_tag="VKL21_00215"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMB44221.1"
                     /translation="MTTLIEMAHAGIITREMKSVAEKEGINAQTVRKLIAQGLIVIPR
                     NLKSKSMPIGIGKLMSTKINANIGTSRDNVDIDAEIEKAITAQKYGADTIMDLSTGGD
                     LNLIRRRILDSVSVPVGSVPIYQAAGKSIENMTSDDMFNAVRKHAKDGIDFVTIHAGV
                     TLSSYERLKNSDRILDVVSRGGAFTISWMEHNDRENPFYFEYDYLLEIAREYELTISL
                     GDGMRPGCINDASDRAKFEEFIILGELVKRARAAGIQTIVEGPGHVPVDEIGLSVTAM
                     KHLTGNAPIYLLGPLVTDIAPGYDHITAAIGGTVAGMYGADFLCMTTPAEHLALPDVE
                     DIKEGTIVTRIAAHAADLTKEGVRERARACDREMALARRDLDWSGQFKQAIDPDKAKE
                     IHARSKNPDTCSMCGELCSIKMMRDVLGKQHLKIKGSKNKIDS"
     gene            complement(2338..>3523)
                     /locus_tag="VKL21_00220"
     CDS             complement(2338..>3523)
                     /locus_tag="VKL21_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB44222.1"
                     /translation="PKGYASQVTVYTPANKVLNPGADFLFRLNGVNLSIKELSQFFSD
                     ERSQNETGILFEAVSKLKLEKQNYTIEDIILEVGQNKSKAKWNVINKLETLKETGILS
                     DRSTTISELVQDGRASIIDMKGVNPDLQAMIVAKLCTDIFDARKLNTVPPCMLVIEEA
                     HNFCPEKGFERTVSTDILRTIASEGRKFGLGLMVVSQRPARVDKNILSQCNTQIIMKV
                     TNPNDLKAISKGLEGIGSDVEDDIKSLSPGVAMIVSTYIERPILVDIRTRKSKHGGAS
                     VPVVKDPLAESQRIKTIPAQIDDRSKSIIDPKLADIPGPVNVTQNMIDNTKTIPASRK
                     IIDNARTIMESPGAIINKARSIIEAKKTEPQHEEIKEQIERKKGKRETSLYRKMFGSR
                     KK"
BASE COUNT          823 a          877 c          702 g         1121 t
ORIGIN      
        1 ttcctaccgg gctgtcactc tggaatatat tccttcccat atctacccct acagcgcctt
       61 ctgatattgc gctaaatgcc attttcaggg catccatttc cggaagcttc ttgccccctg
      121 ctattacaac aggaacattc ctgcatgctt ccactacttt cccgaaatct tcggtataat
      181 atgtttttac gatatgcgct ccgatctcaa tgcaaatcct tgaagccaga gcaaggtacc
      241 ttgcgtcgcg gaccatatct ttccccacag cagtaactgc aagaacaggt attccgtatt
      301 tttcaccgtc atcaacaagc tttgacaggt tgtggatcgt ttctttttca aattccccgc
      361 ccacatatac ggagcaggtg atcgcagttg cattgatgcg tatcgcatct tctatgcatg
      421 tcgttattgt ttcattggat aattccttca ggatgctctg gcccccggat acgcgtaaaa
      481 ttatgggtat atccactgca ggatctatcc agttcctgat agctcccctt gttatcatca
      541 aagcatcggc gtacggaagc aacggttcaa cagtttttcc aaggttggaa agcccggtag
      601 tcggaccctg gaaataacca tgatcaacag ctagcattac gcagcgcccg cttttgggct
      661 tgattatcct tgatatcctg ttcttcattc cccagttcat tttaaatacc tccttttcca
      721 ggcctgacaa gcaaatagcc tttttcgatc agccagtgat ggaattcata ggtcgtatga
      781 aggcaatttt cctggcatat ttccctcagg tcattgtaat cttcagagtc ctgtgttttc
      841 ttattcatca tcatctgtac gtgacattga atatcattac agtattccct tcgtttatat
      901 tcttcaaatc ccttaattgg cataatcaca atgcggattc agaatactta aaattttcgg
      961 aaggactccc catagcgtct ccgaaatgcg cgcaatatta cggaaagaat tatactgtta
     1021 ttctggcaca ggtttaagaa tcgattttat tttttgatcc tttaatcttt aaatgctgtt
     1081 tacccaaaac atctctcatc atcttgatag aacataattc cccgcacatg ctgcatgtat
     1141 caggattctt gctccttgcg tgtatctctt ttgccttatc aggatctatc gcctgtttga
     1201 actgcccgct ccagtcaaga tcacgccgcg ccagcgccat ttcgcggtca caggcgcgag
     1261 ctcgttccct gactccttct tttgtaaggt cggcagcatg cgcagcaatt cttgtaacga
     1321 tggttccttc tttgatatcc tcaacatccg gaagggcaag atgttcggca ggcgtggtca
     1381 tgcacaggaa atccgcgccg tacatacctg cgacagtccc gccgatggcg gcggtaatgt
     1441 ggtcatatcc cggcgcgata tcggtgacaa gaggaccaag aagataaatg ggcgcatttc
     1501 ctgtcaggtg cttcattgcc gtaactgaaa gtccgatctc atcaacagga acatgccctg
     1561 gaccttcaac aatggtctgg atgcccgcgg cccttgccct ttttacgagt tctcccagga
     1621 ttatgaattc ctcgaattta gccctgtcag aagcatcatt tatgcagccg ggtcgcattc
     1681 catcacccag gcttattgtc aattcatatt ccctggctat ctcgagcagg taatcatatt
     1741 cgaaataaaa aggattttcc ctgtcattat gttccatcca tgatatggta aaagcgcctc
     1801 cgcggctcac tacatcgagt atcctgtcac tgtttttcag gcgctcataa gaggacaatg
     1861 ttacaccggc atggatagtg acaaaatcaa tcccgtcttt agcatgtttc cttactgcat
     1921 tgaacatgtc atcgctcgtc atattctcga tgcttttccc tgctgcctgg tatatcggga
     1981 cagaacccac agggacacta acagaatcca gaattctcct gcgtataaga ttcaggtctc
     2041 cgccagtcga aagatccatt attgtatctg cgccatactt ttgtgcagtt atggcttttt
     2101 caatttcagc atcaatatca acgttatccc tggaggtccc aatattggca ttgatctttg
     2161 tgctcatcaa ttttccaatg ccaattggca ttgatttgct cttgagattc cgggggatga
     2221 caattaatcc ctgcgcaatg agttttcgca cagtttgcgc atttatccct tctttttcag
     2281 ccacactttt catttccctg gttattatcc cggcgtgtgc catttcaatt aaagtggtca
     2341 tttcttcctc gatccgaaca ttttacgata aagactggtt tcgcgttttc ctttttttcg
     2401 ttctatttgc tctttgatct cttcatgctg gggctcggtt ttttttgctt caatgatcga
     2461 tctggcttta ttgataatgg ctccggggga ttccataata gtcctggcat tgtcaattat
     2521 cttccgggaa gcaggaatag tctttgtatt atcgatcatg ttttgagtaa cattaacagg
     2581 tcctggaatg tcagctaatt ttgggtcaat gatcgatttc gatctatcat cgatctgggc
     2641 gggtatggtt tttatcctct ggctttctgc cagtgggtct ttcaccacag gcacacttgc
     2701 tccgccgtgc ttactttttc gggttcttat atccacaagt atggggcgct cgatatatgt
     2761 gcttacgatc atcgctactc ccggtgaaag actcttaata tcatcttcca catcagagcc
     2821 tatcccttca agtcctttac tgatggcttt caagtcattg ggatttgtca ctttcattat
     2881 gatctgcgta ttgcattgcg aaagaatgtt cttatcaacc ctcgcggggc gctgtgaaac
     2941 aaccatgagc ccaagaccga atttcctgcc ctcggatgca atggtgcgaa gtatatcggt
     3001 gctgacagtt ctttcaaatc ctttttcagg gcagaaatta tgcgcttctt cgatcacaag
     3061 catacacgga ggcacggtat tgagttttct ggcatcgaaa atatcagtgc acaattttgc
     3121 cacgatcatg gcctgaaggt cgggattaac acctttcata tcgattatcg aggcccttcc
     3181 atcctgcaca agttcactga tcgtagttga tctatctgaa agaatacccg tttctttgag
     3241 cgtttcaagc ttgttgatca cattccattt ggctttgctc ttattctgtc ccacttcaag
     3301 aattatatcc tcaatagtat agttctgttt ttcgagcttg agttttgaga ccgcctcgaa
     3361 caatatcccg gtctcgttct gcgaccgttc atccgagaaa aactgcgata attcctttat
     3421 gctaagatta acgccattta atctgaaaag aaaatcagct cctggattga ggactttatt
     3481 cgccggagta taaacggtta cctgcgaggc atatcccttg ggc
//
LOCUS       DATGBN010000011         5934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_233619, whole genome shotgun sequence.
ACCESSION   DATGBN010000011 DATGBN010000000
VERSION     DATGBN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233619"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VKL21_00225"
     CDS             complement(<1..519)
                     /locus_tag="VKL21_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44223.1"
                     /translation="MILIIVLLLLLLIGYINIKNPIVKDIVTVKHAEPTLSAIVTTSD
                     NKITVGAFNIQRFGITKASKPEIMGVLSKIIRNYDVIAVQEIRNLSSQTTLPMLRDDL
                     NSIGSPQYEYVVGKTTNEEQYAYFYNTQTIQLIGSPYRYPYLNDIFQREPYLAEFKVK
                     NGNFDFVLITIHA"
     gene            complement(606..1262)
                     /locus_tag="VKL21_00230"
     CDS             complement(606..1262)
                     /locus_tag="VKL21_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44224.1"
                     /translation="MKKMKIFELVLLVLSIVILSGCTAPQKTQDTQYNGTNQVKNLSE
                     IPDNAKKLLGIDPPGNDTDGDGVDDLNDLTPTFADVPPQASTGVVGFRIQSIFVENNY
                     DEVAKKDAPDHLELVLQSTTNKDIYNLTVYYVITDAKINKKESYIKQLSGFVLKANET
                     RTIHFDAGQKPDHFRFNPNSIYYTTKDELNFTVIINADGYEAQTIQVKKDAGGAEAPD
                     "
     gene            complement(1265..2233)
                     /locus_tag="VKL21_00235"
     CDS             complement(1265..2233)
                     /locus_tag="VKL21_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMB44225.1"
                     /translation="MSNSLVIARKEFKDILHSGIFLTVLLLLLALTVTSVIVSSFVFK
                     SQVDSYQSSLQILKELGKQPGSPQPELFPLNLLRGVVDYLEIVGAILGILLGNLTIAR
                     EKNTQTLKLILTRPVSRKDLLFGKMMGNAVFIGIVLVFVGLFALLSLVFVAGVALTLT
                     EAVKLVLVIAISWIYIMAFISLAAFLSLSMRSLSNALVVAFTIWLVFVLILPQIGDTM
                     DPDNQVPRGFFNSIHFNKEQGKQVLTKFGSYENARNFIEELSVTKHYERTAFALLGIK
                     PEFNGLPVPDILGQKWFDILVVILFFSAGILTNYSVLNKKDILVWD"
     gene            complement(2237..3157)
                     /locus_tag="VKL21_00240"
     CDS             complement(2237..3157)
                     /locus_tag="VKL21_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMB44226.1"
                     /translation="MNNIITIARHEIQSLFRERTFILLLAVFAAMTLASAYIGWSSQH
                     TINQVYTAAAEQMRQSGKVAPSSPFADYPHLALLKNMIIYVIVIGGLLAISTAYSAGM
                     RERKAGVLKLLFSRPVGKNDFLLGKLLGISFVLAVVMLLAAAISALSSAVLVSLNAQD
                     MIHLFSFYGFSLVYLLGFAFIGFSSALVKEDEVMALLIPIIFWISITFVLPQLTSALY
                     PTGSLNPTLPQTDILQSPILQTMHDAVYPFSISQHYKEAAATILNINAIGPDAQVHSG
                     ILNVVTTFCWMLLCLTASFIAVYSFDKSRW"
     gene            complement(3150..3926)
                     /locus_tag="VKL21_00245"
     CDS             complement(3150..3926)
                     /locus_tag="VKL21_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB44227.1"
                     /translation="MRTDFNSKNTETGGKQFIINIRSLSKKYGDHFAVNDLNLKVAEG
                     EIFGFLGHNGAGKTTTVNMLTTLLQPTSGGAFVAGFDILRESVEVRKRIGYLPENVKL
                     YDSMTAYENLEYFAKLSGISKPKERILLVLDFLDANGYRDKKIGALSKGMLQRIGIAQ
                     AILHEPKLLFLDEPTSGLDPFGVKQLRDTLLRLNKEHGMTLFMNTHLLSEVTKTCSTI
                     GVLSHGKLIHSDSLANTMRRFSDESSLEQVYLTIEEGRNE"
     gene            complement(4125..4844)
                     /locus_tag="VKL21_00250"
     CDS             complement(4125..4844)
                     /locus_tag="VKL21_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB44228.1"
                     /translation="METFDQYDITFKNKQILELGPGNSIALAMNFLCNSSGKYLMVDK
                     YPRILKTEKQAKHLLEQITYFEEKYHSNLNNFINKDNLEFNEAFLTYMQDSAEDLRNI
                     SSHSVDIILSISVLEHIRDVEKSFKEMRRILKEGGVMYHSIDLRDHYNSNEPLKFLKY
                     SDFAWDNFLTKEGYSYTNRLRIYDYMELLAKYGFEIININKIIYDSDLTEQKSFIKKF
                     RDEDKENLKVLSIEILAKKRF"
     gene            complement(5297..5575)
                     /locus_tag="VKL21_00255"
     CDS             complement(5297..5575)
                     /locus_tag="VKL21_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44229.1"
                     /translation="MEAHPKVGDTYQQEFAKDAKDAKDKATVLSLGKSICVPYGCFSH
                     LLQTRDFSPLEPSITEHKYYAPGVGQIKVVMVKGGSEEEHLVKIKKGE"
     gene            complement(5612..>5934)
                     /locus_tag="VKL21_00260"
     CDS             complement(5612..>5934)
                     /locus_tag="VKL21_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44230.1"
                     /translation="DVKEHYDAAKFTNPTEIDNKYYPLEPGTTFIYKGTKEGQTIRDE
                     VIVTHKTKVIEGVTTCVIRDNLYVNGTLSEKTDDWFAQDDAGNVWYFGEFTTEIDTGS
                     HEGS"
BASE COUNT         1623 a         1241 c         1222 g         1848 t
ORIGIN      
        1 tgcatggata gttataagca caaagtcgaa gttgccattc tttactttaa actctgcaag
       61 atatggctct ctctggaaaa tgtcattcaa atagggatac ctgtaaggac tgccaattag
      121 ctgaattgtc tgtgtattgt agaaatatgc atattgctct tcatttgttg tctttccgac
      181 aacatattca tactgtgggc ttcctattga attcaaatca tctcttaaca tgggtaaagt
      241 tgtctgcgat gataagttac gaatttcttg aactgctata acgtcatagt tcctgataat
      301 cttcgataaa acccccatga tttcgggctt gcttgctttt gtaatgccga atctctgaat
      361 attaaaggcc ccaactgtga ttttgttgtc cgatgttgta actattgctg atagggttgg
      421 ttcagcgtgt ttaacagtta caatatcttt tactataggg ttcttaatgt tgatataacc
      481 gattagaagt aataacaaga gcactataat aagaatcata agaatcaata aagctgattt
      541 attagatttc ttcaatatcg tattaagatt cgggtctggc atcatggggt cctgtatttt
      601 taaatctaat ccggtgcttc tgcaccgcca gcgtcttttt tcacctggat tgtttgggct
      661 tcatagccgt ctgcgtttat tataacagtg aagttcagct catcttttgt tgtgtaatat
      721 atgctgttcg ggttgaagcg gaagtggtct ggcttctgtc cagcgtcaaa gtgtattgtt
      781 ctggtttcat tagccttcag gacaaaccca ctcaactgct ttatgtaaga ctccttcttg
      841 tttattttcg cgtcagtaat gacataataa accgtcaagt tgtatatgtc tttgttggtt
      901 gtgctctgca ataccagctc cagatggtca ggtgcatctt tttttgctac ctcatcataa
      961 ttgttttcta caaatatgct ctgtatcctg aaacccacta caccagttga agcttgtgga
     1021 gggacatctg caaaggtggg tgttaagtcg ttcaaatcat ctacaccgtc gccatcagta
     1081 tcattgcccg ggggatcgat tcccaatagt tttttagcgt tatcgggtat ttcggacagg
     1141 tttttgactt gatttgttcc attatattgc gtatcttgcg tcttttgtgg tgcagtgcag
     1201 cctgatagga tcactatcga gagaacaaga agtactaatt caaatatttt catctttttc
     1261 atgtttaatc ccatacgagt atgtcttttt tattcaatac agagtaattt gtaaggatcc
     1321 cggcagaaaa aaacagtatg accaccagga tatcaaacca tttttgacca agtatatctg
     1381 gtactggtag gccgttaaat tccggtttta tgccaaggag agcaaaagcg gtgcgctcat
     1441 agtgtttcgt cacggataat tcctcgatga agttcctggc gttctcatat gagccaaact
     1501 ttgtaagtac ctgttttccc tgttccttgt tgaagtgtat gctattgaag aaaccccggg
     1561 gtacctggtt atccgggtcc atcgtatccc ctatctgggg cagtattagg acgaatacca
     1621 accatatggt gaaagccact acgagggcgt ttgaaagact cctcatactc agggaaagga
     1681 atgcagccag gctaatgaac gccataatgt aaatccatga aattgcaatc accagcacta
     1741 actttacggc ttcggttaaa gttaatgcaa cgcctgcgac gaaaacgaga cttaaaaggg
     1801 cgaataagcc cacaaatacc agcacaatgc ctatgaacac agcatttccc atcatcttcc
     1861 cgaataacag gtctttgcgg ctgactgggc gtgtaaggat gagtttgagt gtctgagtgt
     1921 ttttttctct ggctatggtg aggttgccaa gcaggatacc gagtattgca cccactattt
     1981 caaggtaatc tacgaccccg cgaagcaaat tcagcgggaa taactccggt tgtgggcttc
     2041 ctggttgttt tccaagttct ttcaggatct ggagagaaga ttgatatgag tcaacctggc
     2101 ttttgaatac aaaagaggag actatgacag atgtgactgt taacgccaga aggagcagga
     2161 gaacagtaag gaatatgccg ctgtggagta tgtctttgaa ctcttttctt gcgataacta
     2221 aggaattgct catggttcac catcgagatt tgtcaaatga atacacagcg ataaagcttg
     2281 cagtgaggca cagcagcatc cagcagaatg ttgtgacaac attaagtatg ccagaatgaa
     2341 cttgtgcatc cggaccgatt gcattgatgt taaggatggt cgctgctgcc tctttgtaat
     2401 gttgtgagat agagaaagga tatacggcat catgcatagt ctggagtata gggctctgga
     2461 ggatgtcagt ctgcggcagt gtcggattca acgaacctgt gggataaaga gcagatgtca
     2521 actgcggaag tacaaacgtt atcgagatcc agaaaattat gggtatgagg agagccataa
     2581 cctcatcctc cttgacaagg gcagaactga atccaatgaa agcgaaacca agcaagtaaa
     2641 ccaatgaaaa accatagaaa ctaaacaaat ggatcatgtc ctgggcattg agacttacca
     2701 gaactgcaga agataatgcg cttatagcgg cggcaaggag catcaccacc gccaggacaa
     2761 aggatatccc aagcaatttg ccgagcagga agtcattttt tcctacgggc cttgaaaaaa
     2821 gcagttttaa cacccctgct tttcgttcgc gcattcctgc tgaataagct gtgcttatgg
     2881 caagcagtcc tccgatgacg ataacgtaga ttatcatgtt cttgaggagg gcaaggtggg
     2941 ggtaatctgc aaatggcgag gaaggggcga cttttccgct ctggcgcatc tgttcggctg
     3001 ctgcagtata tacctggttt atagtatgct gggatgacca gccgatgtaa gctgatgcga
     3061 gcgtcatggc ggcgaagacc gccagaagca gaataaaagt ccgctcccgg aacagtgact
     3121 ggatttcgtg gcgagcgata gtgataatat tattcattgc gtccctcttc gattgtcagg
     3181 tatacctgtt caagagatga ttcatcgcta aagcgtctca tggtgttcgc cagggagtca
     3241 gagtgtatca gttttccatg gcttaatacg ccaatcgtgg aacacgtctt cgtaacctct
     3301 gacagtagat gagtgttcat gaatagggtc ataccatgct ctttgttcaa gcgtagcaga
     3361 gtgtcacgta attgtttcac cccaaaaggg tctaatcctg atgttggttc gtcaaggaac
     3421 agcaattttg gttcgtgaag tattgcctga gctatgccta tcctttgcag catacccttt
     3481 gacaatgcgc caattttttt atcgcgataa ccgttagcat ccaggaaatc aagcaccagg
     3541 aggattctct cctttggttt gcttattccg gagagttttg caaaatactc aaggttctcg
     3601 taagctgtca tcgaatcata taacttgaca ttctcaggaa gataccctat tcgtttgcgc
     3661 acttcaacgc tctccctcaa tatatcaaaa ccagccacga aggcgcctcc ggaagttggc
     3721 tgcagcaatg tagtcagcat attgactgta gttgtcttac ccgcaccatt atgacccagg
     3781 aacccgaata tctcaccctc tgccaccttc aagttcaggt cgttaactgc aaaatgatca
     3841 ccgtatttct ttgaaagaga ccgaatgttt attatgaact gttttccccc agtttctgta
     3901 tttttcgaat taaagtctgt tcgcatatga atatttctcc gggtcagcac ctgcccttca
     3961 agctgttatt tccatcctta aaaacttcct tgatcacgcc tgcatttaat cttcgatttc
     4021 atttcttgca ttgtaataag aaatctgtac ttaaaccatt tttgggatgc ttatcgaaaa
     4081 atagaacagt tatcgaatgg tcatgttagc tatattctca gttcttaaaa tctttttttt
     4141 gctaaaattt caattgaaag aacttttaaa ttttccttat cttcatctct gaatttttta
     4201 ataaatgatt tttgttctgt caaatctgaa tcataaataa ttttgttaat attaattatt
     4261 tcaaaaccat atttcgctaa taattccatg taatcataaa ttcgaagcct atttgtatag
     4321 gagtaccctt ccttagtcag aaaattgtcc caggcgaaat cagaatattt aagaaatttc
     4381 aacggttcgt tggaattata atggtctcgc aaatcaatac tatggtacat aacccccccc
     4441 tctttcagga ttcttctcat ctccttgaaa gacttttcca catctcttat gtgctccaat
     4501 acacttatgc tcagtattat atcaactgaa tgggaagaga tgttccttaa atcttctgcg
     4561 ctatcttgca tatatgtaag aaacgcctcg ttaaattcca gattatcctt atttataaag
     4621 ttattaaggt tagaatgata tttctcctca aaataagtaa tttgttctaa aagatgtttt
     4681 gcctgtttct ctgttttcaa aatacgcgga tatttatcaa ccatcagata ctttccggag
     4741 ctgttgcata aaaagttcat agccaatgca atcgagttac caggaccaag ttctagaatt
     4801 tgtttatttt tgaaagtaat gtcgtattga tcaaaagttt ccaccagctt tgtgaaaaga
     4861 gcatctatat gcttgaaatc actgcaatac atctgccaga acattatttt tcgaagcagg
     4921 tctcttatta gaggaacccg agtatacgta aatctgataa tagaatcaca tttattgaat
     4981 atatgtttta aaattcccaa cttgaacctg ccagaagctg atgacgatga ggtcaaggga
     5041 gctatttcca agttaacaac ctgtacttag tttttccgta tttcctctca tgaccttatc
     5101 tgctgtgtgc atttctatta ccaataagaa atatctactt aaataatttt tggtatgctt
     5161 atcgaataat gtaacagtta tctaattgat caggcaaaat actaaagctt gaaatgtcca
     5221 tcaagatgtc agacttttcc aacgtcaagg aaatctctgg acatttcagc taaaatcact
     5281 cattccgcca aagccgttat tctcccttct taatctttac cagatgctcc tcttcggatc
     5341 cacccttgac catgacaacc ttgatctgac cgacacccgg ggcatagtat ttatgttccg
     5401 taatgctagg ttcgagcggc gaaaagtccc tggtttggag gagatgggag aagcacccgt
     5461 aaggaacgca gattgatttg cccagactga gcacggtggc tttgtctttg gcatctttgg
     5521 catctttggc aaactcttgt tggtaggtgt cgccgacctt cggatgcgcc tccatgacaa
     5581 taccgggttt ggctccgttc acacctgttt tctaggaacc ttcatggctg ccggtatcaa
     5641 tctcggttgt gaattctcca aagtaccata cattccccgc gtcatcctgt gcgaaccaat
     5701 cgtctgtttt ttccgatagg gtgccattca catacaggtt atcacgaata acgcatgtgg
     5761 tgacgccttc aatcaccttg gttttatggg taacgatgac ctcgtcccgg atcgtctgac
     5821 cttccttcgt ccctttgtat ataaaagtgg tcccaggctc caaaggatag tacttgttgt
     5881 caatctcagt tggatttgtg aactttgcag catcgtaatg ttccttaaca tcat
//
LOCUS       DATGBN010000012         1667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_389045, whole genome shotgun sequence.
ACCESSION   DATGBN010000012 DATGBN010000000
VERSION     DATGBN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389045"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VKL21_00265"
     CDS             complement(<1..1396)
                     /locus_tag="VKL21_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HMB44231.1"
                     /translation="MPENIIFGIDIAKGSLHAREKPAYAVVILKNGDIEHHKMVSLHK
                     FMRMAWKEKPSLIAADNIYEIVSDKQDLISFLRKLPPNTKLVQVTGGEQLLPLTKLAR
                     EKGLTFDRFDPDAEALICARLAQMGTGYEVSAFEDKTMIKVSRGRSPGRGGWSQNRYR
                     RKMHGYVRQRSREIEDALNELSRTSGVTYSQNKTERFGGYSKSEFIVDAPRNLIHIGS
                     GKYGDVQVSVKGIERDALVFKPLKGKKRDYIIVGIDPGTTTALAVLTLDGELRMLHSS
                     RTISIPEVIEMIAQEGKPLVIASDVNPPPNAVEKIRRAFSAVSSSPFEVLLAQDKIDF
                     AAPYSYSNNHERDAIAAAISFYRKNKNKFEQIRKKIPPGLDASEAIAQVVRGKSVEVV
                     ISGLTRTETKEQDVHAVSVRKDEEVFHLRESIRKYESIINEMKIHQDFLKNELALRDN
                     KIHELEEQIKRQRSE"
     gene            complement(1400..>1667)
                     /locus_tag="VKL21_00270"
     CDS             complement(1400..>1667)
                     /locus_tag="VKL21_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HMB44232.1"
                     /translation="EPDWYLDEILGQIKKTYGLGIIHGDLSEFNIFVNPGGCEIIDWP
                     QFITPSHRNAQELLKRDIDNVLSYFNRKYRIKRDIEEIIKSIVA"
BASE COUNT          393 a          407 c          322 g          545 t
ORIGIN      
        1 cttcggaccg ctgccttttg atctgttcct ccaattcgtg gatcttatta tctctcaaag
       61 caagttcatt ttttaagaag tcctggtgga tcttcatttc attgattata ctttcatatt
      121 ttcgtattga ttccctcaaa tgaaatactt cttcatcttt tctcacggaa actgcatgga
      181 catcctgctc tttggtttca gtcctggtta atcctgatat cacaacttcc acggatttac
      241 ccctgacaac ctgggctatc gcttcgctgg catctaatcc gggtggtatt tttttcctga
      301 tttgttcgaa cttattcttg tttttccggt aaaacgatat ggcagcggct atggcatcgc
      361 gttcatggtt gttggaataa ctatacggag ccgcaaaatc gattttatct tgcgccagga
      421 gtacttcaaa aggagaactt gatactgcgc taaacgccct ccggatcttt tcaacagcat
      481 tcgggggagg gtttacatca cttgcgataa caagcggctt tccctcctgt gctatcattt
      541 ctatgacttc aggtatcgaa atggtccttg aactatgaag cattcgcaac tctccgtcaa
      601 gtgtaagaac ggcaagagct gttgtggtcc cgggatcgat cccaacgatg atataatccc
      661 ttttttttcc tttaagcggc ttgaaaacca gagcatcccg ttctatgcct tttacactta
      721 cctgcacatc accatatttt cccgaaccga tatggatcag gtttcttggc gcatccacga
      781 tgaactcgga ttttgaatat cctccaaacc tctctgtttt attctgggaa tatgtgaccc
      841 cactggttct tgaaagttca ttcagggcat cctcgatttc ccttgatctt tgcctgacgt
      901 aaccgtgcat tttacgcctg tacctgttct ggctccatcc ccctctcccg ggtgagcgcc
      961 ccctgctgac cttgatcatt gttttatctt caaatgcgga tacctcatag cctgtgccca
     1021 tctgggcaag ccttgcgcat atcagggctt cagcatcagg atcgaacctg tcaaatgtga
     1081 gtcctttttc gcgggcaagt ttagtcagcg gcaaaagctg ctcgcccccc gttacctgga
     1141 caagtttggt gttgggaggt aatttccgga gaaaagaaat aaggtcctgc ttatcggaaa
     1201 ctatctcata gatattatcc gcagctatta atgagggttt ttctttccag gccatacgca
     1261 tgaatttatg gaggcttacc attttgtgat gctcaatatc cccatttttg agtatcacga
     1321 cggcataggc tggtttttcc ctggcgtgca aagaaccttt tgcaatatct ataccaaaaa
     1381 ttatattttc cggcataatt catgctacaa tacttttaat tatctcctct atatcccttt
     1441 tgatcctgta tttacggtta aaatatgata atacattgtc aatatcccgc tttaataact
     1501 cctgtgcatt cctgtgagat ggagtgatga attgaggcca atcgatgatc tcacaccccc
     1561 cgggattgac aaagatgtta aattcactaa gatcgccatg aatgatccca agtccgtatg
     1621 ttttttttat ctggcccaat atttcatcca gataccaatc cggttca
//
LOCUS       DATGBN010000013         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_39498, whole genome shotgun sequence.
ACCESSION   DATGBN010000013 DATGBN010000000
VERSION     DATGBN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39498"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..3015
                     /gene="mgtA"
                     /locus_tag="VKL21_00275"
     CDS             463..3015
                     /gene="mgtA"
                     /locus_tag="VKL21_00275"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HMB44233.1"
                     /translation="MNNISKKTNSTNQRPQAFWSLTAAELLAQLQTTPLGITSDEARQ
                     RLTRFGSNLLTPRKRSDSLALFLVQFKSPITLILLFAAGLSFFLHEHVDVLIILAIVF
                     ISGLLGFWQERGAANAIEKLLAIVQIKATVLRDGNPIEIPVEEIVPGDVIILNSGDII
                     PGDCLILESKDFFVDEATLTGETYPVEKSAGELVKETQLGQRNNSLFMGTHVVSGSAK
                     AVVVCTGTETEFGKVSERLKLRPPETEFERGVRRFGYFLMEITLLLVIAIFAINTYLG
                     RPILESLLFSLALAVGLTPQLLPAIISVNLAHGAKRMAEQKVIVKRLASIENFGSMNV
                     LCSDKTGTLTEGVVRVHSTLDVEGKESEKVLFYAYLNAFFETGFTNPIDSAIRNYRQF
                     DMSDCQKLDEVPYDFIRKRLSILVSKDDKHLMVIKGALSNVLSVCSSAQTSDGKIVNI
                     ANVKEQIQQAFEKQSSMGLRILGVAYRDVGSDSLITKDHEAAMIFSGFIVLFDPPKAG
                     IADTINKLNHLGVSLKVITGDNKLVAANVSQQVGFSNPEILTGSDLRSMSDEALIKRV
                     NDISIFAEVEPNQKERIILALKKSGNVVGYIGDGINDASALHAADVGISVDSAVDVAK
                     DAADIVLLEKDLGVLVQGVREGRMTFANTLKYVFMATSANFGNMFSMAGVSLFLPFLP
                     LLPGQILLTNLMTDIPEMTIATDSVDVEMVDHPRRWDIGFIRNFMLVFGILSSVFDIL
                     TFSALFFILHATSDQFRTGWFMESVISASMIVLVIRTKKPFFKSKPGKYLSTATLLIV
                     ASALLFPFLPVGKVFGFIQLPISFLLLVGVIVAFYIIAAEIVKTVFYRMVKF"
BASE COUNT          899 a          719 c          754 g          960 t
ORIGIN      
        1 tcgagggcta acacccctgt tttataccca tattactccg tatggacaat tccaattgga
       61 tatgaataaa cgattggata ttgaaggaac agtgtaacgc atcaagtggt gtctggttgc
      121 agcagcgctg gtactacccc aaattgcata tatatttagg tttgtttttc aagatattgt
      181 ctgattgtag ggatacaaaa tttaatcgtc actaaattat gaggcctatt ggcttgtttc
      241 tttccaaatt aaagattatg tttcaattat catacgtttc taccacacct gattttggat
      301 tataaatata tgaaaaatcc agcagcatgt tactgggcac tacacttagc gtccatattt
      361 tgggtattta tacgtctctt taaattgaca atatcaatca ttcgaaaaga taatatctat
      421 cttattcaag aataagttaa cagtcccgga tttacctgaa acatgaacaa tatttctaaa
      481 aagactaatt caacgaatca acgccctcag gcattctgga gcctcacggc tgctgaacta
      541 cttgcgcaac tccagacaac accattgggg attaccagcg atgaggcacg gcagcgactc
      601 acacgttttg gctccaatct cctgacgccc agaaaaagat cggactcact tgcccttttt
      661 cttgttcaat tcaaaagccc cattaccctc atccttctct ttgcggcagg attgtcattt
      721 ttcctgcatg aacatgttga tgttctcatt atccttgcaa ttgttttcat aagcggcctt
      781 ctcggattct ggcaggagcg aggagcggct aatgcaatcg aaaagttgct cgctatcgtg
      841 caaataaaag ctacggtgct gcgtgatgga aacccgatag aaattcctgt cgaagagatc
      901 gtgcccgggg atgttattat cctcaacagc ggagatatca ttcccggaga ctgcctgatc
      961 cttgaatcaa aagacttttt tgtcgatgaa gccacgctga caggcgaaac atatccggta
     1021 gaaaagtcgg ccggggaact ggtgaaggaa acacagcttg gtcagcggaa caacagcctt
     1081 tttatgggga ctcatgttgt gagcggcagc gcgaaagccg tggttgtctg cacagggaca
     1141 gaaaccgaat ttggcaaggt ttccgaacgg ttgaaactgc ggccaccgga gacggagttt
     1201 gagcgcggcg tcaggagatt cgggtatttc cttatggaaa ttaccctcct gttggtgatt
     1261 gcaatttttg caatcaacac ctatctggga cgcccaatac tggaatctct tcttttttcc
     1321 ctggcactcg ctgttggact gacccctcaa ctgctgccag cgattatcag cgtgaacctt
     1381 gcacacggcg caaagcgcat ggcagagcag aaagtgatcg ttaaacgcct tgcctctatt
     1441 gaaaattttg gtagtatgaa cgtgctttgt tcagataaga ccggcactct gaccgaaggg
     1501 gtggtgcgag tgcactccac tcttgatgtt gaaggaaagg agagcgaaaa agttcttttc
     1561 tatgcttatc tcaatgcttt tttcgagaca ggtttcacga atccgataga cagcgcgatc
     1621 aggaactatc gccagttcga tatgtccgat tgccagaaac tggatgaagt gccttatgat
     1681 ttcattcgta agagactgag catccttgtc tcaaaagacg ataagcacct gatggtgata
     1741 aaaggcgcac tttcaaatgt gctatcagtt tgttcatcag cgcaaacttc ggatggaaag
     1801 attgtgaata tagccaatgt gaaggaacag attcagcagg cctttgagaa gcaaagcagt
     1861 atgggcttgc gcatattggg tgttgcctac cgggatgtcg gatctgattc gcttataacc
     1921 aaagaccatg aagctgcgat gattttttca ggttttattg tcctgtttga tcctccgaaa
     1981 gctggaattg ccgacactat aaacaaatta aatcatcttg gcgtctcatt gaaggtcatc
     2041 acaggggaca ataaactggt tgctgccaat gtcagtcaac aggttggatt ctcaaatccg
     2101 gaaatcctta ctggttcaga ccttcgctca atgagtgatg aagcgctgat caagcgagtg
     2161 aacgatatca gtatattcgc agaagtcgag ccaaaccaga aagaacgcat cattctggct
     2221 ctgaaaaaga gtggaaatgt tgtaggctat attggagatg gaattaatga cgcttcagcc
     2281 ctccatgctg ccgatgtcgg catatctgtt gatagcgccg tggatgttgc caaagatgct
     2341 gccgatattg tactgctgga aaaggattta ggtgttcttg ttcagggtgt gcgcgaagga
     2401 agaatgactt ttgccaacac cctcaagtat gttttcatgg ccacaagcgc caatttcggg
     2461 aacatgttca gcatggctgg agtttcgctg ttcttacctt ttctccccct gttgcccgga
     2521 cagatcctgc tcaccaatct catgactgat atcccggaga tgacaatagc taccgatagc
     2581 gtggatgtcg aaatggttga tcatcccagg cgctgggaca tagggttcat ccgcaatttt
     2641 atgctcgtct ttggaatctt gagttccgta tttgacattc ttacattcag cgctttgttt
     2701 ttcatccttc atgcaacatc tgaccaattc aggaccggct ggtttatgga atcggttatt
     2761 tcagcttcga tgatcgtgct tgttattcgc accaaaaaac ctttcttcaa gagcaaaccc
     2821 gggaagtatc tgtccacagc cacactgctg atcgttgcaa gtgcacttct tttccctttc
     2881 ctgccagttg ggaaagtatt tggatttata cagcttccga tttcgttcct tcttcttgtg
     2941 ggagtgattg tggcctttta catcatcgcg gccgaaatag tgaaaacggt attttacagg
     3001 atggtgaaat tttaatcttg acttcgccag atagcctata aaggggaaat cacatttcag
     3061 aagattttgt gaacaacctt gcatgaaaat gtaactttcc caataggggt agtaccagcg
     3121 ctgctgcatc ctggggccat gtaaatccat tctatgaatt tttaacttgt tcaatcgtat
     3181 ggcagagacg gagagatcgc ggtaaacaat agagattatc aggaaattgc tattttatcc
     3241 ttgcatctat tgcagatttg tatggtctac atctatagca tcgataaacc gttggatttt
     3301 aaccgaaaat aagccattag tatataaagc at
//
LOCUS       DATGBN010000014         6372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_505869, whole genome shotgun sequence.
ACCESSION   DATGBN010000014 DATGBN010000000
VERSION     DATGBN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505869"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VKL21_00280"
     CDS             complement(<1..293)
                     /locus_tag="VKL21_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44234.1"
                     /translation="MSQSKIMIEFKNNEIIIGKKLSINDEQVLDFSEILAKNNIRYVL
                     FSRYIPILFGNNETSEDIDILIQKISFEKFLKLWLEIENTYECKNTSDPIDAY"
     gene            complement(268..927)
                     /locus_tag="VKL21_00285"
     CDS             complement(268..927)
                     /locus_tag="VKL21_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HMB44235.1"
                     /translation="MKVFIDHREMRSRVAEGLVKLGAEIEITTLEIGDYVVSDRVAFE
                     RKTVDDIFSTLLERRELFSQLFDLAKSYRKPILIIEGEDIFFFSSRKVNPKAIQGFLN
                     TIALMRIPTLYTLNEAETAQVISMIALKEQNDKTRPFNFHGKRSRLSQIEMKEYIISS
                     LPGIGQVTSRNLLNHFGSVENIVTAPREELMKVERVGFKTAKRIREIAGGQYESKQNN
                     D"
     gene            complement(1165..2181)
                     /locus_tag="VKL21_00290"
     CDS             complement(1165..2181)
                     /locus_tag="VKL21_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HMB44236.1"
                     /translation="MKYAELSMKFRKFFELPSSPVAIRIINDSSEQKTSSQPMRFCEM
                     VRRSAFYGESFIFSVEELTCTSAELALGFTEPSYGEVYPRIKPANTKLVSVSSLEKTE
                     KKPDVVVIVGNPRKIMRISTILSQLHEKQPVEVKFKGEFAVCGECTAIPFMEKKVNLS
                     LLCNGARMFSGYRDDEIVMGFPLDDFVRVSESTEEKEITSALCGCIMDDIPAGAVSAI
                     EKIGFGKGTDQFFGRFGNEIVRLYTPKEKDGKITSLTLHVPIKFKDSQTASIVNDKAK
                     EILEKPLLHRVRDNWVDIALPLDLGETLNRASMRGEKFQALVKSGIETILKETEKIKR
                     KTAG"
     gene            complement(2183..3370)
                     /gene="pscS"
                     /locus_tag="VKL21_00295"
     CDS             complement(2183..3370)
                     /gene="pscS"
                     /locus_tag="VKL21_00295"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HMB44237.1"
                     /translation="MDLNKYKNLTRGISDTYVNVHPIQRGGVLTAEARKALLEFGDGY
                     SVCDYCFESRVDLVDKPPVHDFTADVAEFLNMDDVRFTAGCRHAKWAVMHMVCEPGDT
                     VVVDALAHYTSYLAAEANKLNIVEVPHNGYPRFEIDARGYASKFEEVEQKTGKLPSIA
                     VLTHVDYRYGNIADAQEVGKICKEYGVPFLLNTAYSSGIMPVDGKKLHVDFLCGSGHK
                     SWAASAPIGILATTYEWSSKVFNKSTCRGDWSGRGFSKKEVALFGCSPVFGAPIATLM
                     ASFPAVVERVNRWDEEVKNAQFFVNELEKIEGFHQMGVKPKQHPLMNFESQPLHEIAQ
                     KDKRRGYFLYHELKKRKIVGLHPGMSKNFKINTYGLKREQIEYVASAFKDIARENGIK
                     VEE"
     gene            complement(3447..4709)
                     /locus_tag="VKL21_00300"
     CDS             complement(3447..4709)
                     /locus_tag="VKL21_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44238.1"
                     /translation="MSSFFNDDTASIEIPIRLVVYVILTGAILGIFAIGLSNIWPGIT
                     TNTMEKQIGEIKVSLTALQNGGARNLIDHDSPSGNIRTIKITIPEGVDYLAFGADPDP
                     DNDLNLTNTPEGMLTERGNVIFYSGKSGKIRIPLGEFIDLREGRLENGRWVVNEIGGK
                     QFGTVISGKGNYELTFELVYDPISKEKYTLVHMTDELNAYISPHDPAVLPNNLWVSVN
                     PGYIPADGVTKAEILVKLKDKKGRDASSSGVNINLSVSLGNLSGSNLTTIKGKATAAI
                     SSDMVGTSLITATSPGLNPGSSYLTIKPEPIILEFNRWIYDEDEKLNGQFFTEQELDY
                     NILFTGYGSKFSVPLAGVWWPNVSIVIDGVQLGEEIIDSESTISRTFNQATLPAGNHN
                     LDISLKNDKYLPLLGDTNIYIGKIVLSG"
     gene            5063..5599
                     /locus_tag="VKL21_00305"
     CDS             5063..5599
                     /locus_tag="VKL21_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44239.1"
                     /translation="MKKSNEEKNNEQANSDIHGRIEYIDQGLRSVDSRIRAIEKRLSA
                     KTFEPDHVNIPQDKSGNIDEELLVKLDALDEKLKHLEKTIQETKENEIVPIRTQLSMI
                     EGRTLKLENQNKITIGKIKVPIEFSGLATTIVMFATGYLIYSDHWNIIRSSYYPIAIG
                     ILFGVVVIGKFIMTNSNR"
     gene            complement(5615..6031)
                     /locus_tag="VKL21_00310"
     CDS             complement(5615..6031)
                     /locus_tag="VKL21_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44240.1"
                     /translation="MALGIPIKIVVLTIVGMAGLAAMLVIIDNGESAIPKPIHADLKS
                     SNIIILPAFNDTEDIDMEVEVIDSIEGTPVEKASVVLSGLGAISVNMTNKDGYATLRF
                     KRTDFDLKAGEGYLQLDVKANGFQDYSNEYAVKIVI"
     gene            complement(6043..>6372)
                     /locus_tag="VKL21_00315"
     CDS             complement(6043..>6372)
                     /locus_tag="VKL21_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44241.1"
                     /translation="IKINNGAVQEGSIATVGGEGDYKVKVNLKVYDKNNNPVDKASVT
                     LSGGGGFGADQTNSYGEAIVTLGDTKSTNVTFRPNQESIDLKIAVKANGFYDYEDGNA
                     IQIYSKN"
BASE COUNT         1719 a         1403 c         1221 g         2029 t
ORIGIN      
        1 ttgtatgcgt ctatgggatc gctcgtgttt ttgcattcat atgtattttc aatttccagc
       61 cataatttca ggaatttctc aaatgaaatt ttttggatca ggatatcaat atcttcgctt
      121 gtttcattgt ttccaaagag aattggaatg tatcttgaga atagcacgta ccggatatta
      181 tttttggcaa gaatttccga aaaatccaat acttgttcat catttattga tagctttttg
      241 ccaatgataa tttcattgtt cttaaattca atcattattt tgctttgact catattgccc
      301 tcctgcaatt tccctgatcc tttttgcggt cttgaaacca acacgctcca ctttcatgag
      361 ttcctcccgc ggcgctgtca cgatattttc cacgctgcca aagtggttta gcaaatttct
      421 tgatgtcacc tgtcctatac ccgggagtga tgatataata tattccttca tttcaatttg
      481 agagagacgg ctgcgttttc cgtgaaagtt gaaaggtctg gttttatcgt tttgctcttt
      541 caaagctatc atactgataa cctgtgctgt ttctgcttcg ttaagcgtgt agagagttgg
      601 gattcgcata agggcaatgg tattaaggaa tccctgtatc gctttggggt tcaccttgcg
      661 acttgaaaag aaaaatatat cttcaccttc gataatcaaa atgggcttcc tgtaggactt
      721 tgccaggtca aaaagttggg aaaatagctc ccggcgttca agcagggttg agaaaatatc
      781 atcgaccgtc tttctttcga atgccacgcg atcgcttacg acataatcac ctatttccag
      841 ggtcgttatc tcaatttctg cgccaagttt aacaagacct tccgctactc tggaacgcat
      901 ttctctgtga tcaatgaaaa ccttcatact ctcctcgaca aaacattatg caaacaacat
      961 aacatatact ctctcaaaga gcaaggtgaa actaatcatg aacctgatgt ctttattcgt
     1021 cttggccgtg aaaatcaggg ctttgcctct gggactgagt gaaacagcca tgttaatgct
     1081 caacttctat ccttagttat tctgatgata ggacgattat aggaacaaat tgattaggtc
     1141 gaatataaaa acaaaagtaa tatttcatcc cgctgttttc ctctttatct tttcagtttc
     1201 ctttaagatg gtctcgatgc cgcttttgac aagcgcctga aacttctcac cacgcatact
     1261 tgccctatta agagtctctc cgaggtcaag gggaagcgca atatcaaccc aattatccct
     1321 gacccgatga agcaatggct tctcaagtat ttcctttgct ttatcattaa ctatggaagc
     1381 ggtctggcta tctttgaatt ttatgggtac atgaagtgtc agggatgtga tctttccatc
     1441 cttttccttg ggcgtgtata atcgtacgat ctcattcccg aagcgaccga agaactggtc
     1501 tgtccctttt ccgaatccta ttttttcaat tgcagaaact gcgcctgcgg gaatgtcatc
     1561 cataatacat ccgcacaatg cgcttgttat ctctttttct tctgtgcttt cggatacgcg
     1621 aacaaaatca tcaagcggaa aacccattac gatctcatca tcgcggtagc cggagaacat
     1681 ccttgctccg ttacacagaa gtgagaggtt cacctttttc tccataaaag gaatagcagt
     1741 acactcaccg cacactgcaa actctccttt gaactttacc tcaacaggtt gtttttcgtg
     1801 caattgcgac aggatcgtag atatgcgcat aatcttcctg ggatttccaa cgatcacgac
     1861 cacatcgggt tttttctctg tcttttccag ggagctaacg cttactaatt tcgtatttgc
     1921 aggttttatg cgaggataga cctcaccgta acttggctct gtgaaaccaa gagccagttc
     1981 agcgcttgtg catgtgagtt cttctacgct aaagatgaaa ctttccccgt aaaacgcgct
     2041 tcttcttacc atttcacaaa agcgcatggg ctggctgctt gttttttgtt cggagctgtc
     2101 attaatgatc ctgatggcaa caggtgaaga agggagttca aaaaatttcc tgaatttcat
     2161 tgacaattct gcatatttca tcttattcct cgacttttat tccgttttct cttgctatat
     2221 ccttgaaagc gctggctaca tattcaatct gttcgcgctt caagccgtac gtattgatct
     2281 tgaaattctt gctcatgcca gggtgaagac caactatctt tctctttttt aattcatgat
     2341 acaggaaata tcccctgcgc ttatcctttt gggcgatctc atggaggggc tggctttcaa
     2401 agttcataag aggatgttgt tttggcttga cgcccatctg gtgaaatccc tctatctttt
     2461 caagctcatt cacaaaaaac tgtgcatttt ttacctcttc atcccagcga tttacacgtt
     2521 ctaccactgc gggaaatgat gccataagag ttgcaatggg cgccccgaat acaggggagc
     2581 agccgaataa tgccacttct ttttttgaaa aaccgcgtcc gctccagtca cccctgcaag
     2641 tggatttgtt gaaaactttg ctgctccatt cgtaagtagt tgccagtatt ccgataggcg
     2701 ccgatgctgc ccagcttttg tgccctgaac cgcaaaggaa atccacatgg agcttcttgc
     2761 catcaaccgg cattatacct gatgagtaag ctgtattaag caggaacgga acgccatatt
     2821 ctttgcatat cttccccact tcctgcgcat cggcaatatt gccatacctg taatccacat
     2881 gtgtgagcac tgcaatcgag gggagttttc cggtcttttg ttctacctct tcaaatttac
     2941 tggcataccc ccgggcatca atttcaaatc tgggataacc attgtgcgga acttctacaa
     3001 tgtttaactt gttcgcctct gcggccagat atgaagtata atgggcaaga gcatccacca
     3061 caacagtgtc acctggttca caaaccatat gcattaccgc ccatttcgca tggcgacagc
     3121 ctgccgtgaa cctgacgtcg tccatattaa ggaattctgc gacatccgct gtaaaatcgt
     3181 gaactggagg cttatccaca aggtccaccc ttgattcgaa acaataatcg catactgaat
     3241 agccatcacc gaattcaaga agggcttttc tcgcttctgc tgtcaggacc ccgcctcgct
     3301 ggatggggtg cacgttcaca taagtgtcag aaataccgcg agtaaggttt ttgtatttgt
     3361 taaggtccat gctctcaaat catgttccaa gagtttatta atgaacttat aatttacgga
     3421 aaaggcaaga aggatgaaag taactttcaa cccgaaagta ctattttccc aatataaata
     3481 tttgtatctc ccaataatgg aagatatttg tcatttttta aactgatatc taagttatga
     3541 ttacccgccg gtaatgttgc ctgattgaat gttctggaaa tagtggattc actgtcaata
     3601 atttcttcac caagttgaac cccatcgatc actatactta cattgggcca ccagacccct
     3661 gccaggggca ccgagaattt tgatccgtat cctgtaaata aaatattata atctaattcc
     3721 tgttccgtga agaattgccc attgagtttt tcatcttcat catatatcca ccggttgaat
     3781 tcaaggatta ttggttcagg ctttattgtc aaatagcttg aacccggatt taatcccggt
     3841 gaggttgccg ttatcaaaga cgttccaacc atatctgaac taatggcggc tgtggctttt
     3901 cctttgattg ttgtcaggtt cgatccggag agatttccaa gtgatactga taaatttatg
     3961 tttactccgg aagatgacgc atcacggcct tttttatcct ttaacttgac caggatttca
     4021 gctttggtaa ccccgtcggc cggaatgtat cctgggttca cggaaaccca gaggttgttt
     4081 ggcaataccg ccggatcgtg aggacttata tatgcattta attcatcggt catatgaaca
     4141 agcgtgtatt tttcttttga gatgggatca taaaccagtt cgaatgtcag ttcataattt
     4201 ccttttcccg atattactgt accgaattgt tttcccccta tttcattaac gacccatctc
     4261 ccgttctcaa gccgtccttc acgaaggtct ataaattcac caaggggtat cctgatcttc
     4321 ccggatttcc cactgtagaa aataacattt cctctctcag tgagcattcc ttcgggcgta
     4381 tttgtgaggt taaggtcatt atcagggtca ggatcagccc caaaagcaag ataatccaca
     4441 ccttcaggga tcgttatttt gattgttcga atatttccag aaggagagtc atggtcgatc
     4501 aaattacgtg cacccccgtt ttgcaatgcg gtcaatgaga cttttatttc gcctatttgc
     4561 ttttccatag tattggtggt aatgccaggc catatgtttg aaagtccgat ggcaaaaatc
     4621 cccagtattg cgccagtaag gatcacataa actacaagtc ttatggggat ctcaatggat
     4681 gctgtatcgt cattgaaaaa agaggacata tttttcagtt ctaatagggt tcatgttatt
     4741 aaggatttca tccttgacta ttgatgagtt ctaaatactt tcacatcttt taaagagtta
     4801 accacatgtc tgtatcagat atttcaaaag tattgaaaat gatttacgaa gctttgtttt
     4861 ctccatctac gaatgatgaa ttgtataaac cttcagagaa tctaccaagc atgattttct
     4921 attatcttgt tacaatcatc gtagtgattg tggttctcta tcttgtgtta aattatatag
     4981 gctttgattg gagttcattg gggttttgag aaaactttaa ataacttgca tttatttcgt
     5041 gtacttccag gtggcatcta tattgaaaaa aagcaatgaa gaaaaaaata atgagcaggc
     5101 caattcggat attcatggcc ggattgaata cattgaccag gggttgagaa gtgttgatag
     5161 ccgtatacgt gcgattgaaa agcgcctttc ggccaaaacc tttgagcccg atcatgtcaa
     5221 tattccccag gataagagtg gaaatattga tgaagaactc ctggtaaaat tggatgcatt
     5281 ggatgagaaa ttaaaacatc ttgaaaaaac aatacaagaa actaaggaaa atgagatagt
     5341 tccaataaga acgcagctct cgatgataga agggagaacg cttaaacttg agaatcagaa
     5401 taaaataacg atcggaaaaa tcaaagtacc tattgaattt tcaggtcttg caactactat
     5461 cgtgatgttc gcaaccgggt atctcatata cagcgatcac tggaatatta taaggtcatc
     5521 atattatcca atagcgatcg gcatattatt tggggttgtc gtgataggga agttcataat
     5581 gaccaacagt aataggtaat atgacaattt tagatcagat aacaatttta acggcatact
     5641 cattggaata atcctggaaa ccgttcgctt tgacatcaag ctgtaaataa ccttctcctg
     5701 ctttcagatc aaaatcagtc ctcttaaatc tcagggtagc gtacccatcc ttgttcgtca
     5761 tattgacaga aatcgcaccc aggccggaaa gaacaacgct tgctttttct acgggtgttc
     5821 cttctattga atcaattact tcgacctcca tgtcgatatc ttccgtatca ttaaaagccg
     5881 gcaatatgat aatattactg cttttcaggt ctgcatgtat cggttttggg attgcgcttt
     5941 caccattatc tataattaca agcatggcgg ccagacctgc cattcccact attgtaagaa
     6001 ccactatttt tatcgggatt ccaagcgcca tttttttata gcttaattct ttgaataaat
     6061 ttgaattgca tttccatctt catagtcata gaagccgttg gccttaacag caatctttag
     6121 atcgatactc tcctgattcg gcctgaaagt tacattagtg ctctttgtat caccgagtgt
     6181 tacaatagct tcgccgtaac tgttcgtctg atcagccccg aatccacctc ccccgctgag
     6241 tgtgacgctt gctttgtcaa caggattatt atttttatca tatactttta aattgacctt
     6301 tactttataa tcaccttcgc cgcccacagt tgcaatgctt ccttcctgca cagccccatt
     6361 atttatctta at
//
LOCUS       DATGBN010000015         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_428137, whole genome shotgun sequence.
ACCESSION   DATGBN010000015 DATGBN010000000
VERSION     DATGBN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428137"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VKL21_00320"
     CDS             <1..1125
                     /locus_tag="VKL21_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HMB44242.1"
                     /translation="PANCSYCWSSDKGSPIMSIETIKEVVEWLKDFRGDNVTFTFHGG
                     EPLMAGVDFFREALPLLSHGLSDLKPAFALQTNLWKMTPEMALILAEYNIPIGSSLDG
                     PKELTDLQRGKGYFEKTMRGYEIAKANGLKVSFICTFTSQSVKQKEEIFNFFLENGIT
                     LKLHPALPSIRDENPEKYALAPEEYGELLVYLLNKYLENMGMIEIMNIDHLCKCVFTS
                     RGTVCTFVDCMGDTFAVGPDGSIYPCYRFIGMPKYVMGNVKDHPKMEDLAGSDAWKIM
                     FRFKEYVDKNCGECSNIKFCRGGCPYNAMAPHEGEIKSVDPHCIAYKRIFKEIKDRWE
                     NEFFGSPDMPMFGLPSEPEKNAKHGIMPLMMKRIQSHDSG"
     gene            1136..1300
                     /locus_tag="VKL21_00325"
     CDS             1136..1300
                     /locus_tag="VKL21_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMB44243.1"
                     /translation="MGEVVKLQKSGENLVITIPIAICEHLNLKDGEEVELEPFICSGE
                     TGARIRPKRI"
     gene            1522..2865
                     /locus_tag="VKL21_00330"
     CDS             1522..2865
                     /locus_tag="VKL21_00330"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMB44244.1"
                     /translation="MEKSGFKDLKISRDIQKAITDMGFEEPTPIQTQSIPHILEGKDV
                     IGQAQTDTGKTAAFGIATLDRIDPKNLAVQAVILCPTRELAIQVSEELKKLSRYKKGI
                     EILPVYGGQPIDRQIRALKKGVQVIIGTPGRVMDHMERRTLRLDNVKIIILDEADEML
                     DMGFREDIETIMKKIPKERQTILFSATMSKAILDLTKKYQTRPQMIKLAHKEMTVPEV
                     EQFYYEVKSQAKPEVLSRLIDINDLKLSLVFCNTKRRVDELVDNLKSRGYLADGLHGD
                     LQQRQRDIVMSKFRRKEIEILVATDVAARGIDVGDIEAVFNYDIPPDDEYYVHRIGRT
                     ARAGKAGRAFSFVTGREVYRIRQIQQFTKARIIAQKVPSVSDIEEIKTGLVLEKVKGI
                     VDAGHLGEFSNMVEKLTQEDYTSLEVASALLKMVMGEGGREEKITKKNYGKKFES"
     gene            complement(2929..3051)
                     /locus_tag="VKL21_00335"
     CDS             complement(2929..3051)
                     /locus_tag="VKL21_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HMB44245.1"
                     /translation="MGVKKIDEKYRCNICGNEVIVTKAGGGTLVCCGEDMELME"
     gene            3111..3710
                     /locus_tag="VKL21_00340"
     CDS             3111..3710
                     /locus_tag="VKL21_00340"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMB44246.1"
                     /translation="MNNMLKFVLYFLFFAIIHSILAMDYTKNIAGKLMGKGFRYYRIL
                     YSLISIPLLAPAFMIWMIDSKSTPVVYVIPNYLFPVVILVRLIALGMFGYVILQIDLL
                     EFSGIKREKKKELLTGGTYGIVRHPFYTAGILLLIAQMEMTMLDMIAALLVTGYFLAG
                     AFIEERRLLYVFGEEYRKYQKQVPMFIPVKWFAGKLSRI"
     gene            complement(3736..>4100)
                     /locus_tag="VKL21_00345"
     CDS             complement(3736..>4100)
                     /locus_tag="VKL21_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMB44247.1"
                     /translation="IRGALSTVAIDFGVPVLFTRDEEDTAALISVIAKREQAGDSKKE
                     INLHGNKTASTLPEQQEYLVSAISEIGPVVARNLLQHFGSVERIMKASREELMEVELV
                     GPKTADRIREVVSTEYKG"
BASE COUNT         1277 a          785 c          921 g         1117 t
ORIGIN      
        1 cccgctaatt gcagttattg ctggagctcc gataaaggct ctccaataat gagcattgaa
       61 accatcaaag aagtggttga atggctcaag gatttccggg gtgataatgt aactttcaca
      121 ttccatggcg gtgagccgct aatggccggc gtggattttt tccgtgaagc tctacctctg
      181 ctttcccatg gtctaagtga cctgaaaccg gcctttgccc tgcagaccaa tctctggaaa
      241 atgacccctg aaatggccct aattcttgca gagtataata ttcccatagg ttccagtctg
      301 gatggcccca aagagcttac tgacctccag agaggaaaag gatacttcga gaagaccatg
      361 cgcggatatg aaatcgcgaa ggccaatggt cttaaggtga gctttatttg caccttcact
      421 tcccaatccg taaaacaaaa ggaagagatc tttaatttct tccttgagaa tggcataact
      481 cttaaattac atcctgcact tccatcaatc cgtgatgaaa atcctgaaaa atatgcgctt
      541 gctccggaag agtatggtga attgttggtt tatcttctga ataaatatct tgaaaacatg
      601 ggtatgatcg aaataatgaa tattgaccat ctgtgcaaat gcgtgttcac aagccggggc
      661 acggtgtgca ccttcgtgga ttgtatgggg gatacctttg cggtaggtcc tgatggcagt
      721 atctatccct gttatcgttt cataggcatg ccaaaatacg ttatgggcaa tgtaaaggac
      781 catcccaaaa tggaagacct tgcagggtct gatgcatgga aaatcatgtt cagattcaag
      841 gaatatgtgg acaagaattg cggggaatgc agcaacatca aattctgccg gggaggatgc
      901 ccttacaatg ccatggcgcc tcatgaaggt gaaataaaaa gtgttgaccc gcactgtatt
      961 gcatataaga ggatattcaa agaaataaaa gaccgttggg aaaatgaatt ttttggttcc
     1021 cccgatatgc ccatgttcgg tcttccatca gaacctgaga agaatgcaaa acatggaata
     1081 atgccattaa tgatgaaacg tatccaatcc catgattctg gttgaaggtg aaaaaatggg
     1141 cgaagtagta aaacttcaaa aatcaggtga aaacctggtg attaccatac caatagcgat
     1201 atgtgaacat ttgaatctca aagatggtga agaagtggag ttggagcctt ttatttgtag
     1261 tggtgaaacc ggggcaagaa ttagaccaaa acgtatttaa ttcatccacg tatgcctgcg
     1321 aagcatacgt gtttgcgccc ttccgatgcg cgactcggaa tctgcggttc ctttgttgta
     1381 ccggatatat tcatcgaggg ttataattta tcgattgtta tgcagaaata aaaataaatt
     1441 agacagttga cttaaagact tcttttttta tactaacatg gatataagta agataacaca
     1501 caaatcaggg gaagatcgaa aatggaaaaa tcaggattta aagatttaaa aatatcaaga
     1561 gatatacaaa aagcaataac ggatatgggt tttgaagaac ccactcctat ccagacacaa
     1621 tcaattcccc atatactgga gggaaaagat gtgatcggac aggcacaaac agacacagga
     1681 aaaactgcag ccttcggaat tgccacatta gataggatag atcccaaaaa tctggcagta
     1741 caggctgtaa tcttatgtcc aacaagagag ctggccatcc aggtatcaga agaactgaaa
     1801 aagctttcaa gatataagaa aggcatcgag atcctgcctg tctacggcgg acaacctatt
     1861 gaccgccaga taagagcgtt aaaaaaaggc gtacaggtaa ttattggcac acccgggcgc
     1921 gttatggatc atatggaacg ccgaacttta agactggaca atgtaaagat aataatactt
     1981 gatgaagctg atgaaatgct ggatatggga ttcagggaag atattgaaac cattatgaag
     2041 aagatcccaa aggaaagaca gacaattcta ttctctgcaa ccatgtcaaa ggccattctt
     2101 gacctgacaa agaagtatca gaccaggccg caaatgataa aattggcgca taaagagatg
     2161 acggttccag aggttgaaca attctattat gaagtcaaat cacaggcaaa accagaggta
     2221 ctttcccgtt tgatcgatat taacgacctg aagttatcgc ttgtgttctg taatacaaaa
     2281 agacgtgtgg atgaactcgt tgataacctg aaatcgcgag gatatcttgc tgatggactg
     2341 catggtgatt tgcagcaaag acaaagagat attgttatgt ccaaattcag gagaaaagag
     2401 atcgagatac tggttgcaac tgatgtggcg gcgcggggaa tagatgtggg ggatattgag
     2461 gctgttttca attatgatat acctccggat gatgaatatt atgtgcacag gatcggaaga
     2521 acggcaagag caggaaaagc agggcgtgca tttagctttg tgacaggaag ggaagtatac
     2581 aggataaggc agatacaaca attcacaaaa gccaggatca ttgctcagaa agttccttct
     2641 gtaagtgata tcgaagagat caagacaggc cttgtcctgg aaaaagtaaa agggatcgtt
     2701 gatgcggggc atctgggaga attcagtaat atggtagaaa aactcaccca ggaagattat
     2761 acttctttag aagttgcatc agctttgttg aagatggtaa tgggcgaagg aggcagagaa
     2821 gagaaaataa cgaaaaagaa ttatgggaaa aaatttgaat catgatatga aaaacacgat
     2881 caaaaccaag gccagttttt cttttgttga ttgtaaactg gttatttgct actccataag
     2941 ctccatatcc tcaccacagc agacaagggt tcctccgcct gcttttgtca caatgacctc
     3001 gttcccgcaa atattgcatc gatatttttc atctattttt tttacgccca ttgatttaac
     3061 ctcctgataa tatgttatat atggaaataa tatttaacct tatgtggtca atgaataata
     3121 tgctaaagtt tgttctatat ttcctgtttt ttgcgatcat tcatagcatt cttgctatgg
     3181 actatacaaa aaatattgca gggaagctaa tgggaaaagg attccggtat tacaggatac
     3241 tttattcctt gatcagtatt ccattgttag cgccagcttt catgatatgg atgatagatt
     3301 caaaatccac cccggttgtg tatgtgatcc ctaattatct gtttcctgtt gttatccttg
     3361 ttcgcctgat tgcacttggt atgttcgggt acgtcatttt gcaaatagat ctccttgaat
     3421 ttagcggcat aaagcgggag aaaaaaaagg aacttttaac aggaggaacc tacgggatcg
     3481 tcaggcatcc gttctacaca gcgggaattc tcttgttgat cgcacagatg gaaatgacaa
     3541 tgcttgatat gatcgcagcg ttgcttgtaa ccggatattt cctggcagga gcgtttatag
     3601 aggagagaag gttactctat gttttcgggg aggaatacag gaaataccag aagcaagtgc
     3661 ctatgttcat tcctgtaaaa tggtttgctg gaaagctctc aagaatttga aatagggaaa
     3721 tgaaacatca aaatatcaac ccttatactc tgtgctaaca acttccctta tcctgtctgc
     3781 ggtctttggt cccacaagct ccacttccat caattcctcg cgtgatgctt tcatgatacg
     3841 ttccacgctg ccaaagtgct gcagcaggtt ccttgccaca accggcccta tttcagaaat
     3901 agccgaaacc agatattcct gctgttcagg aagtgttgag gctgttttat taccatgaag
     3961 gtttatttcc ttttttgagt cacctgcctg ttcccttttt gctattacgc ttatcaatgc
     4021 ggctgtatct tcttcatctc tggtgaataa tacaggtacc ccgaaatcaa ttgcgactgt
     4081 tgaaagcgcg ccccgaatag
//
LOCUS       DATGBN010000016         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_350555, whole genome shotgun sequence.
ACCESSION   DATGBN010000016 DATGBN010000000
VERSION     DATGBN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350555"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VKL21_00350"
     CDS             complement(<1..882)
                     /locus_tag="VKL21_00350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB44248.1"
                     /translation="MISSNESGKKKKIKIGHLSTLLADPGAACCGVETVIANLIKEQV
                     DQQGHEVTLFASGTSRTRADKLESIYPNFLIQDGITWDKSWPWDLANFANAIKQANNG
                     KIDILQVHSLESLVLPLIDLIEIPVVITIHDIQGILNKTDPGKRFIYQEYSKKANFVA
                     VSNSVKESLSIAGIQPNIVGTIHNGIDIQEYSISSGLRDRLGWIGNITESKGVTDAIR
                     AARALKLPLDIAGPVTSDNEMFFEKEIKPLLRDGIRWVGKIEKHQKPEFFGNLIALLG
                     TSKIPETFGMGILEANAS"
     gene            983..1942
                     /locus_tag="VKL21_00355"
     CDS             983..1942
                     /locus_tag="VKL21_00355"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HMB44249.1"
                     /translation="MSEAFLKTENDSNLMVGEMILIGNVTLENFLIFIFISFLTVVVG
                     NISYALMRRLFDDKLSLRDSKLIARVIEYTLYALGFYYGLLYLLNLELNALIASLGIF
                     SIAIAFGSQQIIQNFIAGLLISVGRPIQLDDWVGIGDTGISNIKDIGLTRTVLRNKDG
                     KLFYVPNSLLISNIIINYTKSGFVEIPVPLTIPYSSDFEKVKNIVKEVAHGHAKILPN
                     VSRVEKKYISKLFELSSIRILFKDKMDMSMFEPRVLISDISDSKTTLSVRIWIREIEK
                     KDEIISEFLEKLLGKFKEENIYKLNSSNAVNLIPPVSTIKQLP"
     gene            complement(1988..2632)
                     /locus_tag="VKL21_00360"
     CDS             complement(1988..2632)
                     /locus_tag="VKL21_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMB44250.1"
                     /translation="MNFRTIIDQFICKKVDIMQEGFKKIILLVTILALYFGGYRLVQT
                     MNPVGINLEIGLDAYIPFIPEFSVIYLLHIPMIIFVFAFYWNDYKTYRSMSFMLIAVI
                     LIALLIYSAFQTEVLRPIIASTDFFSRLTKTIYKYDMSNNTFPSLHVALTTTISAFIY
                     EKNINVGKVLISLTFLIILSTMFIKQHVFLDIIGGLMLAFVIFKIKNVFDIKEI"
     gene            complement(2658..3899)
                     /locus_tag="VKL21_00365"
     CDS             complement(2658..3899)
                     /locus_tag="VKL21_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB44251.1"
                     /translation="MKLNKCFKHAFNMVLHSKLRSWLTITGIIIGVAAVIAIVSIGDG
                     MQQSLNAQLNALGGDIVTISAGFERGGGMFGMRGGTGDHGDSGSQATTREIVLDRSDL
                     QVLKGVPDIALIDTNIRGSVDISYLGKKGAVSVTGVDQSVWSKITTSKIRTGRMLDSA
                     DQNVIVIGGRLASSYFDQPVGINKMVMINGSAFRVVGILDDQGNSVYMPIQMAYQVIP
                     DKTNNIYDTLVVKIKDENLLDEVITKIENKLMTVRHVTAKNKDFSISSRKEMQQTRAD
                     TMNSMNTFLLAIAAVSLIVGSIGIANTMFTSVLEKTKEIGIMKAIGARNQDILSIFLF
                     NAAFIGLVGGILGIILGTILSGFMPALMGGLPLGRGTAIVTLNSIAMALSVSVTVGIL
                     AGIIPAYQASKLKPVDALRYE"
     gene            complement(3918..>5124)
                     /locus_tag="VKL21_00370"
     CDS             complement(3918..>5124)
                     /locus_tag="VKL21_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="COG1361 S-layer family protein"
                     /protein_id="HMB44252.1"
                     /translation="EARIGVENIGSMDINDLMIEVLPEYPFELVSGETAVKSIGIIRG
                     YSEGSNANIKIIKYRMLVNKDTPAGNYELKLKYFQSGSTSSTQKSFFIDVKNRDSAEV
                     IHIDRTALVPGKQSSMNFTINNAGSAPLRDLTFYWENTDNIILPVGSDNTKYIKYIDI
                     GESAELQYQVIADTNAVPGLYKLNLYLTYKENINGTDKKISSIAGVYVGGGTDFDVAF
                     SDNANRQMSFSVANIGSNPANSVSVVIPEQDGWSVSGPNSVIIGNLNKGDYTVASFKL
                     QSSTNMTQNRVARNNSYSRSMQRSTNSSPDTMLMQIAYTDTMGERTLVEKAIKLGIQN
                     IGSANGTTDYQGRRGAVQQQSIFSDYTYYFIGIAVLAGIVLGNRKYRSRKLIDPDFKF
                     KDLFKIEKK"
BASE COUNT         1521 a         1070 c          887 g         1646 t
ORIGIN      
        1 ggaagcgttt gcttcgagta ttcccattcc aaacgtctct ggtattttac tcgttcctaa
       61 caatgctatc aaatttccaa agaactcggg tttttggtgt ttttctattt tgccaaccca
      121 ccttatccca tccctcagca acggtttgat ttctttttca aaaaacatct cattgtcaga
      181 agtaaccgga ccagcaatat ccaatggcaa ttttaatgcc cgagctgccc taatagcatc
      241 agtgacacct tttgattccg ttatatttcc tatccaaccg agcctgtctc tcaaaccgga
      301 actaatcgaa tattcttgaa tatcaatacc gttatgaata gtaccgacga tatttggttg
      361 aatacccgca atgctcagac tttcttttac agaattacta acagccacaa aattggcttt
      421 ttttgaatat tcttgataaa taaacctttt acccggatcg gttttgttca aaattccctg
      481 tatgtcatgt attgttataa ctacaggaat ttctattaaa tcgattagcg gcagcactaa
      541 agattccagg ctatgtacct gtaaaatatc aattttacca ttgtttgcct gcttgatagc
      601 gttagcaaaa ttagctagat cccagggcca tgatttatcc caggtaatac catcctgaat
      661 caaaaagtta ggataaattg attcaagctt atcagccctt gttctcgatg ttccactggc
      721 aaacaaagtt acttcatgcc cttgctgatc tacttgctcc tttatcaggt tagctataac
      781 tgtttctact ccgcaacacg cggctcccgg atctgccaga agcgtactta gatgcccgat
      841 ttttattttt ttcttctttc ctgattcatt gcttgatatc atttctattt cctactatat
      901 tgctggtact aaatattcaa aactccaagt cctaactgag acagactcag tacctttttt
      961 aaacatccga cgagtaaaat taatgtcaga agcattttta aagacagaaa atgattctaa
     1021 cctgatggtt ggagaaatga ttttaatagg caatgtaacg ttagagaatt ttttgatatt
     1081 catatttatt tcctttctca ctgtggttgt aggtaatata tcatatgctt taatgaggag
     1141 attatttgac gacaaacttt cattgcgtga ttcaaaatta atagccagag ttatagaata
     1201 taccttgtat gcacttggat tttattatgg cttgctttat ctacttaatt tagaattgaa
     1261 tgcattaatt gcgtcactgg gtatatttag tattgccatt gctttcggat ctcagcaaat
     1321 aatccaaaat ttcatagctg gattattgat ttcagtaggg agacctatac agcttgatga
     1381 ctgggttgga attggtgata caggtataag caatataaag gatattgggc taacacgtac
     1441 agttctaaga aataaagatg gtaaattgtt ttatgtacca aactctctct tgatatctaa
     1501 tattatcata aactacacaa aatcaggttt tgtagaaatt ccagtgccat taacaatacc
     1561 ttattccagc gattttgaaa aagtcaaaaa tatcgttaaa gaagttgcac atggacacgc
     1621 aaaaatactt ccgaatgtat ccagagtaga aaaaaaatat atctcaaaat tatttgaatt
     1681 gtccagtatt agaatattat ttaaagataa aatggatatg agtatgttcg agccgagagt
     1741 cctgatttct gacatatcag attccaaaac aacgctcagc gtaagaatct ggataagaga
     1801 aattgaaaaa aaagatgaaa taatctctga atttttagag aaattattgg gtaaattcaa
     1861 agaagaaaac atctataagt tgaattcttc aaatgccgtg aaccttattc caccagtatc
     1921 gacaataaaa caacttccat aggttcagat tggcgtcatg ataaagggct tataaatatt
     1981 tcagatatta aatctcctta atatcaaata cattttttat tttgaaaatg acaaacgcta
     2041 gcataagtcc tccgattatg tcaagaaaga catgctgttt gatgaacatt gttgataaga
     2101 ttatcaaaaa agttaaagat attaaaacct ttccgacgtt tatattcttt tcataaataa
     2161 acgctgaaat ggtcgtcgta agtgctacat gcaatgatgg aaaagtgtta ttggacatat
     2221 cgtatttata aattgttttc gtcaaccgtg aaaaaaaatc cgtggatgca ataattggtc
     2281 ttaagacttc tgtttgaaac gcagaataaa tcaataatgc tatcaatatg actgctatca
     2341 gcataaacga cattgaacga tatgtcttat agtcattcca gtaaaatgca aagacaaaaa
     2401 tgatcatagg aatatgcaac agataaatca cggaaaactc tggaataaat ggaatgtatg
     2461 catctaatcc tatttcaaga ttaataccga ctggattcat cgtttgcact aatctgtaac
     2521 caccaaaata tagagcgaga atagttacca gtaatatgat ttttttgaat ccttcctgca
     2581 taatatcaac ttttttgcat atgaattgat ctattattgt cctgaaattc attcaatgtc
     2641 tgttcacatt tcgaacatta ttcatatctc aacgcatcca caggctttaa ttttgaagcc
     2701 tgatacgccg ggattattcc tgcaagtatc ccaactgtaa ccgatacgga taatgccata
     2761 gctattgagt ttaatgtgac tattgcagta cctcttccca ggggaagacc ccccataagc
     2821 gcaggcatga aacctgaaag aattgttcca agtattatac ccaggatccc tccaacgagt
     2881 ccgataaaag cggcattaaa caggaatatg gaaagaatat cctgatttcg cgcaccgatt
     2941 gctttcatga tcccaatttc ttttgtcttc tcaaggacgg atgtgaacat ggtatttgct
     3001 atcccgatag aacctacgat caaggagacc gcagcaatag caagaagaaa tgtattcatc
     3061 gaattcatcg tatctgctct tgtctgctgc atttccttcc tagaagaaat tgaaaagtcc
     3121 ttgttttttg cagtgacatg ccttacagtc ataagtttat tttcgatttt cgtgatcact
     3181 tcatctaaca ggttttcgtc cttgatcttt accacaagtg tgtcataaat attattcgtc
     3241 ttgtctggaa taacctggta tgccatctgg atgggcatat agacgctgtt gccctggtca
     3301 tcaagaattc ccacgaccct aaaagcgctg ccatttatca tgaccatctt atttattcca
     3361 acgggctggt caaaataaga ggaggcaagc cttcctccga taactatgac attctgatcg
     3421 gcagaatcaa gcatacgacc tgttcttatt ttacttgtag taattttaga ccagacactc
     3481 tggtcaacgc ccgtcacgga taccgcccct ttcttaccaa gataggaaat gtcaacgctt
     3541 cccctgatat ttgtatcgat cagcgcaata tcaggaactc ccttcaaaac ctgaagatcg
     3601 ctcctgtcca gtacaatttc cctggtggtt gcctgagagc cggaatcccc atgatctccc
     3661 gtgccgcctc tcataccgaa catgccccct cccctttcaa agccggctga gatcgtcacg
     3721 atatctccgc caagtgcatt cagctgcgca ttaagcgatt gctgcattcc gtcacctatt
     3781 gaaacgatag cgattaccgc ggcaacgccg ataataatgc ctgttatagt cagccagctg
     3841 cgcagcttgc tgtgcagaac catattaaac gcatgcttga aacacttgtt aagcttcatt
     3901 attacctgct ggaattatca tttcttctcg attttaaata aatccttaaa cttgaaatca
     3961 ggatctatca atttgcggct cctgtatttt ctattgccta atacgattcc tgcaagcaca
     4021 gctattccga taaaataata tgtgtaatct gagaatatac tttgttgctg cacagcgcct
     4081 cttctcccct gataatcagt agttccgttc gccgatccaa tattttgaat tccaagtttg
     4141 attgctttct caaccagtgt tctttctccc atagtatcag tataggcgat ttgcatgagc
     4201 attgtatcgg gtgagctatt tgttgatctt tgcatagaac ggctgtaaga attgtttctg
     4261 gcgactctat tctgagtcat attagttgag gactgcagct tgaagcttgc aacggtgtag
     4321 tctcctttgt tcagattgcc tatgatgact gaatttgggc cgctaacact ccagccgtcc
     4381 tgctcaggaa taaccaccga tacggaattt gcgggattgc ttcctatgtt agcaacgctg
     4441 aaagacattt gtctatttgc attatctgag aatgcaacat cgaaatctgt tcccccacca
     4501 acataaactc cggcaatgga cgatattttt ttgtctgtcc catttatatt ctctttataa
     4561 gtcaggtata agttaagctt gtacaatccc ggaactgcat tagtatccgc gatcacctga
     4621 tattgcagtt ctgcactttc gccaatgtca atatatttga tgtacttggt attatcgctt
     4681 ccaaccggaa gaataatatt atctgtattc tcccagtaga atgtcagatc ccttaacgga
     4741 gcgcttcccg cattatttat tgtgaaattc atgctgctct gctttcccgg aacaagcgct
     4801 gtcctgtcaa tatggatcac ttccgcgctg tccctgttct tcacatcaat gaagaaactt
     4861 ttttgagttg aagacgttga acccgactga aaatacttga gtttgagttc gtaattccct
     4921 gccggcgtat ccttattgac cagcatcctg tatttgatta tcttaatatt tgcattggat
     4981 ccctctgaat aaccccggat tattcctatg ctcttaacgg cagtttcacc ggatacaagt
     5041 tcaaaaggat attcaggcaa gacttcaatc ataagatcgt taatgtccat gcttccgata
     5101 ttctcaacac cgatacgggc ttct
//
LOCUS       DATGBN010000017         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_467097, whole genome shotgun sequence.
ACCESSION   DATGBN010000017 DATGBN010000000
VERSION     DATGBN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467097"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1006)
                     /locus_tag="VKL21_00375"
     CDS             complement(287..1006)
                     /locus_tag="VKL21_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44253.1"
                     /translation="MYLFNWDNIPGKDDGKLIEHLEQKYGVDWVRTAKIDKTNDYEIR
                     IFTEKNLLTLKLNTEKNKVTLTINNVITDEFIAKIENSKVNIYTDVEQEKQDIRRGVK
                     LESSYPYSMLGALVLAGLIIVFVMGYYGTQSAGIFSAREAFFVIGASILILTASSAYL
                     LFTLRNTVSVLKIHEEELQSMRDTMQERRISSLESTRSGATTSLGPRVLALEPQLASL
                     ETRVTSLEQKSVSQSGRKSGV"
     gene            complement(1233..1415)
                     /locus_tag="VKL21_00380"
     CDS             complement(1233..1415)
                     /locus_tag="VKL21_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44254.1"
                     /translation="MDKSSEVVLWMEQCAPERLTANKVMLCTRNIFLLENMRDIFIDE
                     YETILKDSGNAGIIKL"
BASE COUNT          457 a          349 c          258 g          565 t
ORIGIN      
        1 ctattctgga tatcaacaat ccagaacaag tttttgtcaa cgaaccaaga atctcccggc
       61 tttagccgtg gagagtgtca aggtgtacgc ccttctatga ttctgagcag tatcaatatg
      121 atagcaatga ccaacaatat atggatcaag ccaccaactg caccgagcga aaatcccaat
      181 agccatagaa tcaacagaat cacaactaat gttgttatta tatccattat cttacctcct
      241 tgacatcttt taccatactt ttttgtttca ttttatcctc cataaattaa actccagact
      301 tgcgtcctga ttgagagaca ctcttttgct ccagggatgt gactcgtgtt tccaggcttg
      361 ctagttgtgg ttccaatgca agaactcgtg gacctagaga cgtggtcgca ccggatcttg
      421 tactctcaag tgaggagata cgcctctctt gcatagtatc acgcatagat tgcagttcct
      481 cttcgtgtat cttgaggacg ctgacagtat tcctcaatgt gaagagcagg taagcagatg
      541 aggcagtcag gattagtatg gaagcgccga tcacaaagaa cgcttcacgt gcggaaaata
      601 tgccagcgct ttgagttcca tagtagccca ttacaaatac aataatcaat ccagcgagaa
      661 ccagagcgcc cagcattgaa taagggtagc tgctttctag ttttactccc cgtctgatgt
      721 cttgcttttc ttgctcaaca tccgtgtata tatttacctt actattttca atcttcgcaa
      781 tgaattcatc agttataaca ttgttaatcg ttaatgttac cttatttttt tcagtgttaa
      841 gtttgagtgt aaggaggttc ttttccgtaa atattcttat ttcataatca ttggttttgt
      901 cgatttttgc tgttcttacc caatcgacgc catacttctg ttctagatgc tctatgagtt
      961 ttccatcatc ttttccaggg atattatccc aattaaataa atacatatct tctactcctt
     1021 gcttgataac aattaattac atttttcata atattttcat tttctccatt tatgctttcg
     1081 ttttcaaata ctaaatctct tagacataat tcatcttttc atctcccttt ttttttgtca
     1141 taatgtttta tgactgttca tacgtaatgt gaggactcgc tggcacacag gacgaatgca
     1201 atagggcaac attttgggcg cttcatatgg atctatagtt ttattatacc tgcatttccg
     1261 ctatctttca atatcgtttc atattcatcg atgaatatgt cccgcatgtt ctcaagcaaa
     1321 aatatattcc ttgtacataa cattactttg tttgccgtga gtctttcagg tgcacattgt
     1381 tccatccaca gaactacttc acttgatttg tccattttct tacccactcc acatattctt
     1441 tttctgtccg atttatacct aaaatgaaag ggaaggaaag ggaaaaaaaa cctttaccaa
     1501 aagttcccac tcttttatat gcatctctat gtacaattta ataatacttt cataacaaga
     1561 tattgcttgt tattacaaaa attcataata aagattatta aagcagccct aatccatcaa
     1621 gttctataa
//
LOCUS       DATGBN010000018         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_991, whole genome shotgun sequence.
ACCESSION   DATGBN010000018 DATGBN010000000
VERSION     DATGBN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..>1516)
                     /locus_tag="VKL21_00385"
     CDS             complement(159..>1516)
                     /locus_tag="VKL21_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HMB44255.1"
                     /translation="EKNRLTPQQGLDRLNKGLNVGIYCLPGGLFIIDEDRENGAFVIP
                     DEVRATFPKTLTIQTRNAGCQYYYKNNGTFNNKIYKFGGIKAGELRANWYYGVAPGSY
                     VKPDDNAGQDATGLYSIINGVSVSPLEKIPAWMEVCEDIPKEKIKIEIENEITWKNDL
                     GMPLYEIRKKSQKLDALLNGANEADRSAADMAAVQLLYFWRFTDSEISRILQEYRAYE
                     KIARQDYIETTISKIIRGERYNPNYKSNGNGKEALENKTPAIEVDTVEDIPIPIEDKI
                     PPISFDGLWEYSYHNFFKDYVASMSDGYPEYAFQNGTATLSTLSRRRLYIRLNGRTEY
                     MNLITLSLGQSGYARKGSAFSAAQRFLRTAVGDTFLSPDLTPEGMISEIADTITTIKK
                     TKSGLEEVKEEKQHGRIRKAVCAWWKDEAGQLYAQLTKPYMQSFKEQICNFYDSPELG
                     W"
BASE COUNT          376 a          334 c          274 g          532 t
ORIGIN      
        1 gtttttttca ccatcattct cagttcgtat attgatatac acaatagata tatacttttg
       61 caatagataa tttaattaag aaagtaagac atcttgaaaa aaaggaacag aaatggtaat
      121 acattttgac taaaatattg cttgaaaaca gctatttttc accagcccag ttcaggactg
      181 tcatagaagt tacaaatctg ctccttgaag ctctgcatat acggttttgt gagttgtgcg
      241 tatagctgcc cggcttcatc tttccaccac gcgcacaccg ccttgcgtat cctcccgtgt
      301 tgtttttctt cctttacttc ttccagtcct gattttgtct tttttatggt cgtaatggtg
      361 tcggcaattt ctgatatcat cccttcgggt gtcaggtcgg gacttaaaaa cgtatctccg
      421 actgcggttc ttaaaaatcg ctgtgctgct gaaaaggctg atccctttct ggcatatcct
      481 gattgcccca agctaagggt gattaagttc atgtattctg ttcgcccatt caaacggata
      541 tatagccttc tccttgaaag tgtggataac gtagctgttc cgttttggaa agcgtattcc
      601 gggtatccgt cactcataga agcaacataa tccttgaaga aattatgata tgaatattcc
      661 cataatccat caaaagatat cgggggtatt ttgtcctcta taggaattgg tatgtcttct
      721 actgtatcga cttcaatcgc cggggtctta ttctctagtg cttcttttcc gtttccattg
      781 cttttataat taggattgta tctttcaccg cgtataatct tagaaattgt ggtctcaata
      841 taatcctggc gcgcgatctt ttcgtaagct ctatattcct gtaatatccg ggatatctct
      901 gaatctgtga accgccagaa atataaaagt tgaactgcgg ccatatctgc ggctgatcta
      961 tcagcctcat ttgcgccatt caataaggca tcaagttttt ggcttttctt gcggatttca
     1021 tacagaggca ttccaagatc atttttccag gttatttcat tctctatctc aattttgatt
     1081 ttttctttgg gtatatcctc acatacttcc atccatgcag gtatcttttc aagcggtgat
     1141 actgacacgc cgttgattat actatataac ccagttgcgt cctgtcctgc gttatcgtca
     1201 ggctttacat aagacccagg ggccactcca taataccaat tagcccttaa ttcccctgct
     1261 tttattccgc cgaatttgta aattttattg ttgaacgtac cgttattttt ataataatat
     1321 tgacatccag cgttcctggt ctggattgtc aatgttttag gaaatgtggc tctaacttcg
     1381 tctggtataa caaacgcgcc gttttcccta tcctcatcaa ttataaacag tccaccaggg
     1441 aggcagtata tacccacatt caagcctttg tttagtcggt caagaccttg ctgtggtgtc
     1501 aacctatttt tttcat
//
LOCUS       DATGBN010000019         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_273111, whole genome shotgun sequence.
ACCESSION   DATGBN010000019 DATGBN010000000
VERSION     DATGBN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273111"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(579..1166)
                     /locus_tag="VKL21_00390"
     CDS             complement(579..1166)
                     /locus_tag="VKL21_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44256.1"
                     /translation="MLPGTIAHETSHAVVALLMGARITKFSVIPSGDTLGHVEYTAPK
                     IPLIGNAAISIAPLIGCPAILLLISRFFGMHFDFPPASFDILAESRFLLDGTLSFITG
                     LDYLNWKTYVFLYLALTLGAGAAPSKTDIISMLPGLIVIIATISALNYSGVNIRYLYD
                     VFSWLSAAISVAMIPLLAVTIIVGMLKLIAVVRSA"
     gene            1808..2158
                     /locus_tag="VKL21_00395"
     CDS             1808..2158
                     /locus_tag="VKL21_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44257.1"
                     /translation="MGWSNFIIIPSLNIAIEVSRDIHDLEDYEEKAFDYLTSEEFDEG
                     IDIETRKIKDLTVRDLTTLFTAYDSTNSLKGLDYNKLLMVWLKSRRIDYDVKSEYDID
                     KEKLEKEGNKIICR"
     gene            2149..2370
                     /locus_tag="VKL21_00400"
     CDS             2149..2370
                     /locus_tag="VKL21_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44258.1"
                     /translation="MPITFTDEDMVYLDKEQDTQLRSIRQAIDALKLKGHTAFCKKCG
                     HLMEYFTVGNAKLLQCVFCGNRVAEMIKL"
     gene            complement(2374..>2756)
                     /locus_tag="VKL21_00405"
     CDS             complement(2374..>2756)
                     /locus_tag="VKL21_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator protein"
                     /protein_id="HMB44259.1"
                     /translation="IMKKLDINQLDDSDEEISEILMSSGLSRPVARTLAYLQKVDTAT
                     SIDLERETGLRQPEVSIAMKQLDPFDWIDESEEKKPGKGRPNKVYSLKVRFKDIIAHL
                     EKEQKKAIDDGMASIKRLKELVRD"
BASE COUNT          851 a          497 c          611 g          797 t
ORIGIN      
        1 ttcaggcgcg tgcttgtgtt caatgatccg aaagtctgcg ataacgtggt cctggatatc
       61 ctgcccgggg gtgtgtctta tgggtataag tggatgccag tatgatggat tttggcaaca
      121 aatgaccggt taggtttttc aggtattgtt aatttttttg tcactggatt catggcagag
      181 cgtgagtatt aatagagtga atcaatggaa taaatgaata gaaaaggtag aggatttcaa
      241 gaattcttgg atagattgtg gataggatag gaagtgaatc ttgtaaagcg ttatgtaatt
      301 atcctctacg cattcaataa ttggtatatg cttaaatagc atttttccaa gataataccc
      361 aaattagctc caaaatcatc aatatatccc tcgttttttc aagcatcttc tttaagggaa
      421 cggcgcgccg tctgcggacg tcggaataat ggggctaaag cccgatcagg ggatgacctt
      481 gcgccgcatg catgcggttt cttgggggcg acagtatttg cttgctggcg acgggaacta
      541 atgcagtggc agggaaaaaa gatggggaag cacagagatc atgccgatct cacaactgcg
      601 atcaacttta gcatcccaac aatgatcgtt acagcaagca aagggatcat cgcaaccgaa
      661 atagccgcac ttaaccagga aaatacatca tatagatatc gaatattgac gccggagtag
      721 ttcaatgcag atatcgtcgc aataataaca attaaacctg gaagcatgct aatgatatcg
      781 gttttgctgg gtgctgcgcc cgctcccagt gtcagtgcaa ggtaaaggaa aacatatgtt
      841 ttccagttca aataatcaag gccagtgatg aaagaaagtg ttccatcaag caaaaatcgt
      901 gattctgcga gtatatcaaa ggaggctggt ggaaagtcga aatgcattcc aaaaaaacgg
      961 ctgattaaca ggagtattgc aggacatccg atcaagggag cgatcgatat ggcagcattt
     1021 ccgatgaggg gtatctttgg agctgtatat tccacatgtc ctaaagtgtc acctgaaggg
     1081 attacgctga actttgttat gcgcgcaccc atgagcaaag ccactactgc gtgactggtc
     1141 tcatgtgcta tcgttcctgg cagcataaga acttgacttc gccagttaac gggtaaaatc
     1201 acaatttttt tgcgtgcatc tctatttcaa atgtgttaat tttggattta ttttccatta
     1261 gttttcccca aaaatccccc ttgttaattt gacacattgt ttatgataaa aatcgataac
     1321 tgaagagact actaagtcaa gagttcccca aaaatactct atttgatttg acacatcctt
     1381 tgagataaat tcatcgtaaa agctatagaa gaagcacctc aaaaaatcca tattggtttg
     1441 acagttgctt tggtttgcca taaaaaacgc tgaaaaattg ttgctttcga tcagatttta
     1501 ggaaaaaggg gatcagttct gcaatttttt agaaaatcaa aaagcaaaaa cctgatattt
     1561 tggcgttaaa tgtccaactg tcaaattgta gaagggaagg atttgcacca ttttggtcga
     1621 agtggcgaag tcaagtaaga acaacgtaca aagttttcga tgctgatgac agggagcgat
     1681 gtaagtattg tgacatcaga tagagcacaa ttgcctggag tggtatgatg tacatgttta
     1741 attaaggaga agcagatgat tgaatatata aataatggaa tgccgaacat aaaaccggag
     1801 aaaagacatg ggctggagta actttataat aattccctct ttgaatattg caatagaggt
     1861 cagcagggat atacatgatc tggaagatta tgaggaaaag gcgtttgatt atctcacctc
     1921 tgaagagttt gatgagggga tagatattga aaccaggaaa ataaaggatt taactgttcg
     1981 cgatcttaca actcttttta cggcatatga cagtactaat tctctcaaag gtctggatta
     2041 taataaatta ctcatggtct ggttaaaaag caggagaata gattatgacg taaaatccga
     2101 gtatgatatt gacaaagaaa aactcgaaaa agaggggaat aaaataatat gccgataacg
     2161 ttcacggatg aagacatggt gtatcttgat aaagaacagg atacgcaatt acgttccatc
     2221 agacaggcga tagatgcttt gaaactgaaa ggtcacacag cattttgcaa aaaatgcggt
     2281 cacttgatgg agtatttcac tgttggaaat gcgaagcttc tccaatgtgt tttctgcggg
     2341 aatagagttg cagagatgat caaattatga gacttaatcc cgcaccaatt ccttcagccg
     2401 cttaatcgat gccattccat catcaattgc ttttttctgc tctttttcaa ggtgagcgat
     2461 tatatcctta aatctaactt taagcgaata taccttattc ggtcttcctt tgcctggctt
     2521 cttttcttca ctctcgtcga tccaatcaaa tggatctaac tgcttcattg ctatgctgac
     2581 ttctggctgg cgcaatcctg tttccctctc aaggtcaata gatgtagctg tatccacttt
     2641 ttgaagatat gccagggttc tggcaactgg tctgctcaag cccgaggaca tgagaatctc
     2701 tgatatttct tcatcgctat cgtcaagttg gttgatatca agtttcttca taatga
//
LOCUS       DATGBN010000020         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_506415, whole genome shotgun sequence.
ACCESSION   DATGBN010000020 DATGBN010000000
VERSION     DATGBN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506415"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..771
                     /locus_tag="VKL21_00410"
     CDS             172..771
                     /locus_tag="VKL21_00410"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HMB44260.1"
                     /translation="METSFFDSTLFIYIVMPLLIFLARICDVSIGTLRIIFVSKGKRN
                     IAPILGFFEVLIWITAISKIMENLDNYINFVAYAAGFATGNFVGMIIEEKLAMGILMI
                     RIFAYERGNELVQTLNARGFGATVVEAHGARENIDLIYSIVKRNELANVLDVISLINP
                     KAFYTIEDVKAVNEGIFTPNKPNSIFPFSNVLRQWRKGK"
     gene            805..1905
                     /locus_tag="VKL21_00415"
     CDS             805..1905
                     /locus_tag="VKL21_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMB44261.1"
                     /translation="MKKILRIALIITKSILSLLLLLLIFVLINSPGKARPFLSKDGKA
                     VAGSIAVIETVNINGLNQRMIIRGRDTTKPVLLYLHGGPGDPEFPFVRQFNSAIEDLF
                     VVCYWDQRGAGLSYSKDIRPETMTLSQFVDDAGTVSEYLIHRFKCKKIYLLGHSWGTL
                     LGSFTVKKYPEYYNAFIAVGQVGDQQQAEKISYDFVLGRARELKDIKAIRTLEKIGPP
                     PYSDPKVAIEKMTKERKYVIRYGGAVKKGNFYPEAVKPLLICREYSFRDKINYLKGMK
                     FSKNYLWDVIMKSNLFKEIPAQQIPIYIMQGTSDYETSYRVAKEYFDALQAPMKKFFT
                     FENSAHSPIFEEPEKFDMILKEILLEQQKDGK"
BASE COUNT          681 a          332 c          401 g          636 t
ORIGIN      
        1 cttaactatt tttggtttat attaattaac aaaaatattg attatttatt tctgtatccc
       61 tattaatcat acaatttaac aaataaaata cagaattgtt taatcaggtt tatggggaaa
      121 aaacataaca acgtaattag tatatttgtt taatttcaaa tactcccgtt tatggaaacc
      181 tccttctttg attccacttt gttcatttat atcgtaatgc cactattaat atttctggcc
      241 aggatatgtg atgtatctat aggtactttg cgtataatat tcgtatcaaa agggaaaagg
      301 aatatcgctc caatcctggg attttttgaa gtactgatat ggataactgc aataagtaag
      361 attatggaga acctcgataa ctatatcaat tttgttgcct atgcagccgg ttttgcaacc
      421 ggcaattttg ttgggatgat tattgaagag aaattagcta tggggatcct gatgatcagg
      481 atttttgcgt atgaaagagg gaatgaactt gtccagacgc tgaatgcaag aggctttgga
      541 gcaacggttg tagaggctca cggagcaagg gaaaatatag atttaatata ttcaattgtt
      601 aaaagaaatg aactcgcgaa tgttctggat gtcatttctc tgatcaaccc taaagcattt
      661 tatactattg aggacgttaa agccgttaat gaaggtatct tcaccccgaa taaaccaaat
      721 agtattttcc ctttcagcaa tgtgctaaga cagtggagga aggggaaata aaagattttt
      781 acttactgat ataaaacata gacaatgaag aaaatcctgc gtattgctct gattattacc
      841 aaaagtattc tatctctctt gcttctccta ctgatttttg ttttgataaa tagtcctgga
      901 aaagcaaggc catttctgag taaggatggg aaggcagttg ctggtagcat tgcagtaatt
      961 gaaactgtga acattaatgg cttgaatcaa agaatgataa taaggggcag agatacaacc
     1021 aaaccggtac tattatatct tcatggcgga cctggcgacc cggagtttcc ctttgtgcgt
     1081 cagtttaatt cagctattga ggatttgttt gttgtttgct actgggatca acgcggcgcc
     1141 ggattgtcat attcaaaaga tattcgtcct gagacaatga ctctttcgca atttgttgat
     1201 gatgcaggaa cagtttcaga atatctgatc catagattta aatgcaaaaa aatatatctg
     1261 ttgggacatt cctggggaac tctgctgggt tcatttacag ttaaaaaata tcctgagtac
     1321 tataatgcat tcatcgcagt cggacaggta ggtgatcagc aacaagctga aaaaatttct
     1381 tatgattttg ttctgggaag agccagggaa ttaaaggata taaaggccat ccggactctt
     1441 gaaaaaatcg gccctcctcc ctattctgat cctaaagtag ccatcgaaaa aatgactaaa
     1501 gagagaaaat atgtcatcag gtatgggggt gctgttaaga aaggaaactt ttacccggaa
     1561 gcggtaaagc cactccttat ttgcagggaa tacagtttcc gggataagat taactatttg
     1621 aaaggaatga agttttcaaa aaactattta tgggatgtta tcatgaaatc aaatctattc
     1681 aaagagattc ctgcacaaca gattccaatt tatatcatgc agggtacatc cgattatgag
     1741 acatcataca gggttgcaaa agagtacttt gacgctctgc aggcacccat gaaaaagttt
     1801 ttcacgtttg aaaactcagc ccattcacca atatttgaag agcctgaaaa gtttgatatg
     1861 atattaaagg aaattctttt ggaacaacaa aaggatggaa agtaaaaata cctaaatttg
     1921 caaatgttaa aatttttgct tcatggatca attcagcaac cacccacttt acaaaagaca
     1981 taatattgat agtgttatga actcactatg ggaattttat aaaaagaaat tcctcatatt
     2041 attcatagtc
//
LOCUS       DATGBN010000021         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_389768, whole genome shotgun sequence.
ACCESSION   DATGBN010000021 DATGBN010000000
VERSION     DATGBN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389768"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1592)
                     /locus_tag="VKL21_00420"
     CDS             complement(99..1592)
                     /locus_tag="VKL21_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB44262.1"
                     /translation="MAELLQFTGNKPEKKRPETGLKESCAHYRELFDNASDCIYTIDL
                     EGQFLTLNNSVVKAMKCDCAQEVLESNMSKWMTPESLEKAKKFMQEVITEKDYYNKSV
                     EIEIICKDGGHVWFEHKARPIKDKNDIIIGLHGIGRDITNKIRLEQKLKESEERYREL
                     FENANDPMYTHDLKGNILTINKVGMKLLGGNEEIIGSNISNWLTPESYKLFEDRVRKI
                     YLDQPLEPTVDIEVINKNGEHKWGEARTRLIKDGDRILAVHGIVRDITEKRKLKQELR
                     ESEDKYRDLFENAQDGMYVLDNERNFLKMNHFGLQTLGCTEEEIIGTNISKWITPDSM
                     KIVDERRVKRLAGEIVDGADVLEIVCKNGEHRWIEIKTREIKDGDKTIEIHGIARDIT
                     QNRLLKQELWKSNKQQKLLCYLIQGTRGGKTRALILKHLADGSFNANQLATALKMDYK
                     TIRHHLNVLIKNGIIGKENNGHSDLYFISNNIEVILNDFNPATIRNR"
     gene            2048..2344
                     /locus_tag="VKL21_00425"
     CDS             2048..2344
                     /locus_tag="VKL21_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44263.1"
                     /translation="MNKSGESIFFNAPAIGLIVGNLGGGKDKEKGGSGLISPYGIDLA
                     IMLGWTFGIFKTFGFTLTPGIAIGRMGFIVISLGIHFQRRVGLGIVIPIFPIWG"
BASE COUNT          653 a          538 c          437 g          874 t
ORIGIN      
        1 ctctaacctt gctgctgatg ggttgcatgc ggcgataaag gattatcttg aaaaaacagg
       61 gaaaaagctg acgtgatttg accagaatat acctatatct aacgatttct tattgttgca
      121 ggattaaaat catttaagat cacttcgata ttatttgata taaaatataa atctgaatgt
      181 ccattatttt cttttccgat tattccattt tttatcagga catttagatg atgcctgata
      241 gttttataat ccatcttcaa agctgtagcc aattgatttg cattaaaaga tccatcagca
      301 agatgtttca ggatcaatgc cctggttttt cctccacggg ttccctgaat cagataacat
      361 agaagcttct gttgtttatt ggatttccag agctcctgtt ttaataatct gttttgagta
      421 atatccctgg ctatgccatg gatctctatt gtcttatcgc catcttttat ttccctggtt
      481 tttatctcaa tccacctgtg ctcgccattc ttgcacacaa tctcaagtac atctgccccg
      541 tccacgattt ctcctgccag gcgtttcacc ctgcgttcat caacgatttt catgctatcc
      601 ggagtgatcc atttagaaat attagtacca attatttctt cttccgtaca tcccaatgtt
      661 tgaagtccga aatggttcat ttttaaaaaa tttcgttcat tatccagtac gtacatgcca
      721 tcctgggcat tttcaaagag gtcgcggtat ttgtcctcag actcccggag ttcctgcttc
      781 agcttccttt tctcagtaat atctcttact atgccgtgga cggctaatat ccgatctcca
      841 tctttgataa gtctggtcct tgcttcaccc catttatgct caccattctt gtttatcact
      901 tctatgtcaa cagtcggctc taaaggctgg tccaagtaaa tcttcctgac acggtcttca
      961 aataatttat aactttcagg agtcagccaa ttagatatat ttgatcctat tatttcttcg
     1021 tttcccccga gtaatttcat tccaacctta ttgatagtca ggatattccc tttcaaatca
     1081 tgtgtataca tagggtcatt ggcattttca aatagttccc tgtatcgttc ttcggattcc
     1141 ttaagttttt gttccaatct gattttgttg gttatgtccc gaccgatgcc atgaagtccg
     1201 attattatgt catttttatc tttgatgggc cttgccttat gttcaaacca tacatgcccc
     1261 ccatctttgc atattatctc tatttcaact gatttgttat aatagtcttt ttcggtaatg
     1321 acttcctgca tgaatttttt cgccttttcc aggctttcgg gggtcatcca ttttgacata
     1381 ttagattcaa ggacttcttg cgcgcagtca cacttcatgg ctttgactac tgaattatta
     1441 agtgtgagga actgtccctc aagatcgata gtataaatac aatcactggc attgtcaaag
     1501 agttccctgt agtgtgcgca ggattctttc agacctgttt ccggtctctt tttttcaggt
     1561 ttattgcctg taaactgcaa aagttcagcc aaaatctcac cggtatcgtt ttttaatttt
     1621 ttcctatcca attcgcaaag attcctgatt ccagccttgc atatataatc tcaattatca
     1681 ttaatcccct tatcatatat agatattact aagctttccg aaaaaagcca tagcgtcatg
     1741 attcgcttgt attatataaa taactacaaa taaattgctt acacactcgc ttctacccta
     1801 aaatggattg aagaaaagat gtcgattcac atgattaaat gtctttttca gacaggatcg
     1861 acaggattta caggatgaac tataaaaatc gtttcctgtt gatcctgtta atcctgtcaa
     1921 agaacaaatt tatgccgcgt cttcccccaa ccagcgtata acaacggatt ttcctgtcat
     1981 gcatttcggt taggagtata gctatcaagg gttaaaaact taagaactca taaactaatg
     2041 tgagtctatg aataaatctg gtgaatctat cttctttaat gctcctgcta tcggtttgat
     2101 agtaggcaat cttggaggcg gaaaagataa agaaaagggc ggtagtggac tcatttctcc
     2161 atacggcatc gacctggcaa taatgcttgg atggactttt ggaatcttca aaacattcgg
     2221 atttactttg actcctggaa tagcaattgg tagaatgggt tttatcgtaa tctctttagg
     2281 tattcacttt caacgacgtg tcggacttgg aattgtaatt ccgatattcc ctatttgggg
     2341 ttagcgaaag cgtaacctgc tcggtaaggg ctggatgccc tgatgtcagc cagcccattg
     2401 tcgtccgttc ttggtttcaa tttatcaagc gtcatatggt acaaacccct taacgaaaac
     2461 tggaatgaca agcctcacag ctttgagcgt gaggtcgctg ac
//
LOCUS       DATGBN010000022         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_467638, whole genome shotgun sequence.
ACCESSION   DATGBN010000022 DATGBN010000000
VERSION     DATGBN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467638"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /gene="tgtA"
                     /locus_tag="VKL21_00430"
     CDS             <1..1246
                     /gene="tgtA"
                     /locus_tag="VKL21_00430"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HMB44264.1"
                     /translation="RERALRDGLHSLIGFDGPIMTDSGSYQLSIYGEVEVNNKEIVEF
                     QQNIGSDIGVPLDIPTPPDVSRIRAESELEQTIIREKEALGLRKDMLLAAPIQGSTFT
                     DLRERSARELSQLDFDVYPLGAVVPLMESYRYSDLVDVIVASKKGLSPASPVHLFGAG
                     HPMMFSLAVALGCDLFDSASYALYAKDGRYMTARGTYHLDQLQYLPCSCPICSSLNAR
                     ELLESEKREELLARHNLYVTFEEIRVVKQAIQEGSLWELVEHRCRAHPQLLSGLKRAL
                     SQHSGWIETIQPLSRSTFFYSGPESSLRPEVLRHRNKLKNLNINGKVLIRARRLENED
                     EFDWVFDFKPPFGPYPLELKETFPFNAEVVDEPDHESLTVALANLIELMKLNPDCEFI
                     TAFDGLYKHPLMTEVQSLSSKY"
     gene            complement(1257..2279)
                     /gene="ftsY"
                     /locus_tag="VKL21_00435"
     CDS             complement(1257..2279)
                     /gene="ftsY"
                     /locus_tag="VKL21_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMB44265.1"
                     /translation="MFEKLKEKLGGFKKALGQVLETKEKEAIATESAKVETEHAPKSL
                     EKFGILDKIKAAVFEQEFIIQESSVKDILWELEMALLENDVALPVAEKIVESIKLELV
                     GTRRKIGSDTGKIVEDALKKAILKVISVDSFDFDGFVRKVNKPVSIVFVGVNGTGKTT
                     TIAKMAERFKSQNYSVVIAAGDTFRAGAIEQIDRHAEALGVKIIKHQEGSDPAAIIYD
                     AIQFAKAKHKDVVLADTAGRMHTNINLMDQLKKVCRVNNPDLIIFVDEAIAGNDAVER
                     AKLFNNAVPFNGSILTKADADSKGGAAISITYTTGKPILFLGVGQTYKDLIKFEPQWL
                     ISRLFE"
     gene            complement(2286..2726)
                     /gene="pfdA"
                     /locus_tag="VKL21_00440"
     CDS             complement(2286..2726)
                     /gene="pfdA"
                     /locus_tag="VKL21_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HMB44266.1"
                     /translation="MSGVLNQQQLAELSEKHQVYQYQAESIAQQINMVKLTIKDVETA
                     LITITALKDEPSGIETLVPIGFGSFVKANLVNTDTIVVGIGAGVSIEKKTEDAKSFLE
                     KRKDELTKYYEQLNNTISKIAQELQNIEKTVQKHQQSQQPMRAE"
     gene            complement(2723..2902)
                     /gene="rpl18a"
                     /locus_tag="VKL21_00445"
     CDS             complement(2723..2902)
                     /gene="rpl18a"
                     /locus_tag="VKL21_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HMB44267.1"
                     /translation="MNFIVKGNFKAGKKWETFTKKLTTLNKNLALEKTFSLLGSEHRL
                     RRRLIKITSVDEAKE"
     gene            complement(3020..4309)
                     /locus_tag="VKL21_00450"
     CDS             complement(3020..4309)
                     /locus_tag="VKL21_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HMB44268.1"
                     /translation="MNTLLAVGVFLFTYALIVIKPKKINEAHAALIGAILSIIFLLKP
                     HHVIEALGFSTLEMPVPLVTTWNVVIILISLMIISSLLDDYGFFEYCAKKAIHASKNS
                     GHRLFLYTYLVVSFIALFAGNDVVILTTTPIILIFCKNALINPKPYMYASFFAANTFS
                     MPLYIGNLTNILIGDSFGLDYFGFSKYMFLPTIAAASVNYHLLKYIFRKQIPDNFNYK
                     DNIKVVIKNNFLVALGLSVLFSVIILGGIANYYKAPISIVASGGALVLLVSERRIAHR
                     LKSISWNVVIFVIGLFIVVKSLDVSGVNGRIGEILFMGLSGNIFVDTYSVSLLSALMC
                     NLVNNIPMTAMMLSIIQQAGLSPAMNTAMGYSLVIGSNLGANFTTFGALAGILWLESA
                     RRYGWNTTITDLLKIGLFVTPIAILGASMILAIELSF"
     gene            complement(4316..4462)
                     /locus_tag="VKL21_00455"
     CDS             complement(4316..4462)
                     /locus_tag="VKL21_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44269.1"
                     /translation="MKYCVDCGYFSTKVKGSNREQYDGSCTRMKKNVYETNLGCKEFN
                     EERI"
     gene            4733..>4990
                     /locus_tag="VKL21_00460"
     CDS             4733..>4990
                     /locus_tag="VKL21_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44270.1"
                     /translation="MKVILGLEFEPKKLTTDMTDYSEFILKNFYKIEGYRIFFPRRNP
                     TFNNPEFENATLRVLIVRLSPLQNIRESITHHFLFQEIRREV"
BASE COUNT         1394 a         1064 c          962 g         1570 t
ORIGIN      
        1 cagagaacgc gccctcaggg atggacttca ttcgcttatc ggattcgatg gcccgataat
       61 gacagactcg ggatcatacc agctatctat ctacggggaa gtggaagtaa ataataaaga
      121 gatcgttgaa ttccagcaga atatcggctc ggatatcggt gtacctcttg atattcctac
      181 accgcctgat gtttcacgaa ttcgcgccga gtctgaactt gaacagacca taatccggga
      241 aaaagaggcc ctgggattaa gaaaagatat gcttctcgcc gcgcccatcc agggttcgac
      301 ttttactgac ctcagggaga gatctgcgcg tgagctaagc cagctggatt ttgatgttta
      361 tcctcttggc gctgtggtcc ctcttatgga atcataccgt tattcagacc tggtggatgt
      421 tatagtagcc tcaaaaaaag gcttatcacc ggcttcaccg gtgcatcttt tcggggcagg
      481 tcatcctatg atgttttctc ttgctgtggc tctgggttgt gatctatttg attccgcatc
      541 ttatgccctc tacgccaagg atggaagata catgacagcg cggggcacat atcatctgga
      601 tcaactccag tacctgccct gttcctgccc gatatgcagt tcgctaaatg ccagggaatt
      661 gcttgaaagc gaaaagcgcg aagaattgct ggcgcgccat aatctctatg tgacctttga
      721 ggaaatacgt gttgtgaaac aggcgataca ggagggaagc ctctgggaac ttgtggaaca
      781 tcgctgcagg gctcatcctc aactgctatc gggactgaag cgcgcgttgt cacaacattc
      841 aggatggatc gagacgatcc agcctttatc aaggtcaacc ttcttttaca gcggtcccga
      901 atcatcactg cgccctgaag ttttaagaca caggaacaag cttaagaatt tgaatataaa
      961 tggaaaggtt cttatcaggg cccgaagact tgaaaatgag gatgaatttg attgggtttt
     1021 tgattttaag ccgccatttg gcccataccc attggagctc aaggaaacat ttccttttaa
     1081 tgcggaggtt gtagatgaac cggatcatga atcattgaca gtggctcttg ctaatttaat
     1141 tgaactgatg aaattaaatc ctgactgtga atttataacg gcgttcgatg gtttgtataa
     1201 acatccgtta atgacggaag tacaaagcct ttcatctaaa tattagcata aaaaaatcat
     1261 tcaaataacc ttgatatcaa ccactgcggc tcgaatttga taaggtcctt ataagtctgc
     1321 ccgactccga ggaacagtat tggttttccg gttgtgtaag ttattgatat tgcggcgccg
     1381 ccttttgaat ctgcgtcagc ttttgtcagg atgcttccat taaatggcac tgcattgttg
     1441 aaaagctttg cgcgctcaac agcatcgtta ccggctatgg cctcatcaac gaaaataatc
     1501 aggtcaggat tgttcaccct gcaaaccttt tttaattgat ccataaggtt tatgttcgtg
     1561 tgcattcttc cggccgtgtc tgcaagcacg acatccttat gctttgcttt tgcaaattgt
     1621 attgcatcat atattatggc agcaggatcc gagccttcct ggtgcttgat aatttttacc
     1681 ccgagagctt ccgcatgcct gtctatctgc tcgatagcgc ctgcgcgaaa tgtatcgcct
     1741 gccgcgataa cgaccgaata attctgcgat ttgaatcgtt ctgccatttt tgcgatagtt
     1801 gtggtcttgc ctgtcccgtt cactcccaca aaaacaatgc ttacaggttt attaactttc
     1861 ctgacgaacc cgtcaaaatc aaaagaatcc actgaaataa ctttcaggat ggctttcttc
     1921 agggcgtctt caactatctt acccgtatca gagccaattt ttctcctggt tccgacgagc
     1981 tcaagtttta tcgattcaac tattttctcc gcaacaggaa gcgccacatc attctctaga
     2041 agcgccatct caagctccca aagaatgtct tttacgctgc tttcttgtat tatgaattcc
     2101 tgttcaaaaa ctgcagcttt tattttatcc aatatgccga atttttcaag agatttcggc
     2161 gcatgttcag tttcaacttt tgcagattct gttgctatgg cttctttttc ttttgtttct
     2221 aaaacctggc caagtgcttt tttaaaacca ccaagtttct ctttgagttt ttcaaacatg
     2281 tgaaattatt cagctctcat cggctgctgg gattgctggt gcttttgaac tgttttttca
     2341 atattttgaa gttcctgggc aatctttgaa attgtgttgt tcagctgctc ataatatttt
     2401 gtaagttcat cttttctttt ttcaaggaat gactttgcat cctctgtttt tttctcaatg
     2461 ctcactcccg cccctattcc gacaacaata gtatcagtat ttacaaggtt cgccttgacg
     2521 aatgaaccaa aacctattgg tacaagtgtt tctatccccg acggttcgtc ttttaaagct
     2581 gtaattgtga tcaatgcggt ctccacatcc ttgatggtca gtttaaccat atttatctgc
     2641 tgggcaatag attccgcctg atattgataa acctggtgct tttctgataa ttctgctagt
     2701 tgctgctggt tcaatacacc actcattctt tagcctcgtc tacagaagtg atcttgatca
     2761 gcctgcgtct taacctgtgc tcgcttccaa gaagggaaaa tgtcttttct aacgcaaggt
     2821 tcttattcaa ggttgttaac ttttttgtaa atgtttccca tttttttcct gctttgaaat
     2881 ttcctttgac tataaaattc ataatttcac ctgatttggg gtttgagtac tacttaaaga
     2941 gatatatatc tactgttaat cggatatgtg tcctttccat agcatcatga ttcgattgga
     3001 ttttataaat aaccatcaat taaaacgaca gttcgatcgc aagtatcata cttgctccaa
     3061 ggatagctat tggtgtgacg aataaaccga tttttagaag atccgttatt gttgtattcc
     3121 atccatatct tctcgcgctt tcaagccaga gtatcccggc gagcgccccg aatgtcgtaa
     3181 aattggcgcc caggtttgaa ccgataacaa gcgagtatcc cattgctgta ttcatagccg
     3241 gggataaacc tgcctgctgg attattgaga gcatcatggc tgtcatagga atattattta
     3301 caagattgca catgagggca gataagagag acacagaata cgtatctacg aaaatatttc
     3361 cgctcaaccc catgaataat atttcaccta tcctgccatt tactccgcta acatccaggc
     3421 tctttactac gataaaaagc ccgataacaa atatcacaac attccatgat atgcttttta
     3481 gcctgtgtgc gatccttctt tcagacacca atagaaccag agcgccgcct gaagccacaa
     3541 tagatattgg cgctttataa taattcgcta tgcctccaag aataattacg ctaaagagga
     3601 cagaaagtcc aagagctaca aggaaattat tttttattac aaccttaata ttatctttat
     3661 aattaaaatt gtcaggtatt tgtttcctga atatatattt caataaatga taatttacag
     3721 aagccgctgc aatagtggga aggaacatat attttgaaaa tccgaaataa tccagaccga
     3781 agctatcccc tataaggata ttcgtaagat ttccgatata taacggcatt gaaaaagtat
     3841 ttgccgcaaa aaaagaagca tacatataag gtttgggatt gatcagggca ttcttgcaga
     3901 agatcagaat aattggagtg gtggtaagga tgacaacatc atttcctgca aaaagcgcta
     3961 tgaaggaaac cacaagatag gtatataaaa acagcctgtg tccgctgttc tttgaagcat
     4021 gtatcgcctt ttttgcgcag tactcgaaga aaccgtaatc atcaaggaga cttgagatta
     4081 tcatcaggga tatgagaatt ataaccacat tccatgtggt cacaagaggc actggcattt
     4141 ccagtgtcga gaaaccaaga gcctcgatta catgatgcgg tttgagcagg aaaattatgg
     4201 aaagtatcgc tccaattaag gctgcatggg cctcattgat ctttttcggt tttatgacta
     4261 tcagtgcata agtaaataaa aaaacaccaa ccgccagaag cgtattcaat tgtgcctaaa
     4321 ttcgttcttc attgaattct ttgcatccaa gattggtttc atatacattt tttttcatcc
     4381 tggtgcagga accatcatat tgttccctgt tgcttccttt tacttttgtt gagaagtatc
     4441 cgcaatcaac acaatacttc aagattcatc ctccgatttt ttataacaaa ttatcataac
     4501 tggtttagta tatttttata aataatatta acaataattc aaacaaaatt caattggcat
     4561 tttctttaag aaaaatattt ctcgaaccaa atacttcact ggtttctatt atgcaaaaac
     4621 ttattaataa atcttactct ctgaattaat aacatcatga tgatgatttt ttgacactct
     4681 ccatgcctga aggcggaagt ttcttgtttc gcttgtaaaa ggcattatgt ccatgaaggt
     4741 catattgggc ttagaatttg aacccaagaa attgaccaca gatatgaccg attattccga
     4801 atttattttg aagaattttt ataagataga aggataccgc atcttttttc ctcgcaggaa
     4861 tcctaccttc aataatcccg aattcgaaaa tgccactttg cgagtattga tagtccgtct
     4921 ctctccactt caaaatatcc gcgaatctat tacacaccac tttcttttcc aggaaattcg
     4981 aagagaagta
//
LOCUS       DATGBN010000023         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_195756, whole genome shotgun sequence.
ACCESSION   DATGBN010000023 DATGBN010000000
VERSION     DATGBN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195756"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            781..1428
                     /locus_tag="VKL21_00465"
     CDS             781..1428
                     /locus_tag="VKL21_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HMB44271.1"
                     /translation="MINEDLWILELKATAVSPLYTGENKLESQKRRKQGNLLPTRMSG
                     DGFASISIFGAIRGYAEKIYKGDGTCDTGKDTKGCGRCLTCDMFGNLGRKGRVSFEDL
                     KSIRPFDKVVERTVHPHIDRETGTISAGKGASIELEEIVEGTELTGRIIIKNPTEKDI
                     EVLNAALAAAEDNGIGGWTRRGKGRVKFEVNAKKVKWANYKEQGAAEAKKLVSMK"
     gene            1425..2471
                     /locus_tag="VKL21_00470"
     CDS             1425..2471
                     /locus_tag="VKL21_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44272.1"
                     /translation="MKPLAFKIQGELENQSELHIATNNTYNERGSQIKESDMYPLGQN
                     LRGAFGYLFLEMKSKIADTLENGHPVIYFKDAMIKHHDGTFIPVVKLDKGIRQVVYEC
                     SECLHIDKYPIIREPMAGISLGSGGTVKNMYMTDVVSSKNRFRFEAIFSMKAKNAVEE
                     KLNEEYLAEFISALKYVEDNGLYLGKRNSKGMGKVLLKKLTILPITMDDIKKRAKTIS
                     QIVRDEDGKMWIHLFSDTISSFPLSGEDIVRDAKNAAKFFDPDFSQYKDPKITHIEKP
                     VELMVNVSFLDLKVKTGKPKFDAPKSVISRGTRFRYQVTDAVPEFFNALAMAELLRGM
                     GDRTSFGKGEFAVS"
     gene            2519..>2746
                     /locus_tag="VKL21_00475"
     CDS             2519..>2746
                     /locus_tag="VKL21_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44273.1"
                     /translation="MLAKSWSCRWGKNGKLYSRERAYEVDKNALITWRIFPVCATTPV
                     YVTPKHKDCCFILCVITIKEGPLPPDEANRLI"
BASE COUNT          911 a          484 c          618 g          733 t
ORIGIN      
        1 atagaaaaat gctattctat tttagcatcg tctcaaaaaa ctcacatctt tgcttctttt
       61 gtagcttttg tcaaattaga agttagaaga ttgaaggaac atatttcatg gtatgagcag
      121 aaagctgttt ttggtagata cgcagttact aattacctaa aggcaaacgc gtaagtccta
      181 tgtttattaa aattgcaatt atcgatacag gattgttttc tatattgctt attagaattt
      241 caatttctgt aataattttt gtcacaatat ctatttcggt cataatattc actctccctt
      301 acctaaattt taaaggcata gtcactaatt gaaatggttg tgcgcattat cttcaatagt
      361 gatttccact agctagggta tcgtcccgat ttctgcgaca tgtacgctaa ggtattaaca
      421 cttcactaaa tcagttattt ttgctcaacg aacccaaaaa gctaaataag gtttttttaa
      481 gcttaatatt ggccaaatag tgattctggt ctggcttctg ctattatccg aataatccat
      541 atctacatct gagagtctgg caactgttcc tgaaaaaagt gcaaatttgg tttatgaagc
      601 tttcatttct ttttatctta atcgttatac tccgcagctc tgctgcgact gggacaaggc
      661 attccaactg caacctcagt cgaacacgga gtcatgagat accccgcagc tctgctgcga
      721 ttgggagctt catttaaagc tgggtaattt tcatataaaa attagaacgg agtcaaaaca
      781 atgataaatg aagacttatg gatattagaa ctcaaagcaa ccgcagtgtc accgctctat
      841 acaggtgaga ataaactgga aagtcagaag agaagaaagc aggggaatct actgccgact
      901 aggatgtcgg gggatggatt cgcatcaatc agtatcttcg gagctatcag ggggtatgca
      961 gagaagatct ataaaggaga tggaacttgc gatactggaa aagacacgaa aggttgcggg
     1021 cgatgcctaa catgcgatat gttcggaaat ctgggacgaa aaggaagagt atcctttgaa
     1081 gatttaaaat ctatcagacc ctttgataaa gtagttgaaa gaacagtgca tccgcatatt
     1141 gacagagaaa caggcactat atctgctgga aaaggcgcgt caattgagct tgaagagatc
     1201 gtagaaggaa ctgaactgac aggcaggatt atcatcaaga accccactga aaaagatatt
     1261 gaagtcctga acgctgctct tgcggctgct gaggataatg gaataggtgg atggacacgg
     1321 cgtgggaaag ggcgagtaaa gtttgaagta aatgcaaaaa aggtaaaatg ggcaaactac
     1381 aaagaacaag gggcagcgga agctaagaaa ctggtgagca tgaaatgaag cctctggctt
     1441 ttaagataca gggagaactt gaaaaccagt cagagctgca tatagccacc aacaatacct
     1501 ataatgaaag gggatcgcag ataaaagaaa gcgatatgta tccgttagga cagaatctaa
     1561 gaggagcatt cggatacctt tttcttgaaa tgaaaagcaa gatcgcagac actcttgaga
     1621 atggacatcc tgtaatctat ttcaaggatg ccatgataaa acatcatgac ggcactttta
     1681 ttcctgtcgt taaattggat aaaggcataa gacaggtggt ttacgaatgc agtgaatgcc
     1741 tgcacataga taaatatccc ataattagag agccaatggc tggaatatcg cttggcagtg
     1801 gaggaactgt caagaatatg tacatgaccg atgttgtctc aagcaaaaac aggttcagat
     1861 tcgaagcgat atttagcatg aaggcaaaga acgccgtaga agaaaagctc aacgaagaat
     1921 atctggcaga atttatttca gccctgaaat atgtcgagga taatggtttg tatctcggca
     1981 agcgcaactc aaaaggaatg ggaaaagttc tcctcaagaa attaacaatc ctgcccatta
     2041 cgatggatga tattaaaaag agagcaaaga ctatttccca gatagtaagg gatgaagatg
     2101 gaaaaatgtg gattcatctt ttctcagata ccatcagcag cttcccgcta tcaggtgaag
     2161 atattgtaag ggatgcaaag aatgcggcaa agttttttga tccagatttt tcgcaataca
     2221 aagatccgaa aataacgcat attgaaaaac cagtcgaact tatggtcaat gtaagttttc
     2281 ttgacctgaa agtaaaaacc ggaaaaccca aatttgatgc gccaaagagt gttatttcca
     2341 gaggcacacg atttaggtat caggtgactg atgcagttcc agagttcttt aatgcgctgg
     2401 caatggcaga gctgctaaga ggtatgggag atcgaacaag ttttggaaag ggggagtttg
     2461 ctgtaagcta agcgattgat atgcattaat gaataccttg ttctgaggat taggtacgat
     2521 gctggcaaaa tcttggagtt gcaggtgggg taaaaatggg aaactttact ccagagaaag
     2581 agcttatgag gtcgataaaa acgctctcat cacatggcgc attttccccg tttgtgcaac
     2641 aacacccgta tatgttacgc caaaacacaa ggactgctgt tttattttat gtgttatcac
     2701 cataaaagag ggacctctgc ctcccgatga agccaaccgt ttgata
//
LOCUS       DATGBN010000024         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_1459, whole genome shotgun sequence.
ACCESSION   DATGBN010000024 DATGBN010000000
VERSION     DATGBN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..429
                     /locus_tag="VKL21_00480"
     CDS             184..429
                     /locus_tag="VKL21_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HMB44274.1"
                     /translation="MFKFSEHARKRMISREISEEDVIAAIENGELEFTRIDEKGRGTE
                     YTHSIELKDPFWRKIMVGWTYDGDDFLILTLYEIKRK"
     gene            437..775
                     /locus_tag="VKL21_00485"
     CDS             437..775
                     /locus_tag="VKL21_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMB44275.1"
                     /translation="MTTIICGKCKQEMREIELEQYEFERGIVLDNVRAMRCPQGHVTF
                     TEEQALNMEQRTEDIKKHAFGFVRSVSKSARSLVIRIPADLAKHLDITENSKIEMIPL
                     GKKKFMVQVK"
     gene            complement(810..2078)
                     /locus_tag="VKL21_00490"
     CDS             complement(810..2078)
                     /locus_tag="VKL21_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HMB44276.1"
                     /translation="MAQTISEKIFSKATSKKVKAGDFVLANIDCAMTHDITGPLAVEG
                     FREIVKGKKNRTVWDPNKIVILFDHQVPADSLNAAANHIFLRQFAKEQGILNYDVFEG
                     VCHQVMPEKGHVRPGDLIVGSDSHTFAYGALGAFSTGIGSTDMAFVFATGKLWFRVPQ
                     TFRFEVEGKLPKCVYPKDVILHLIGDVGVEGARYMTSEFCGSTIRKMDIPGRMTMSNM
                     AIEMGGKAGIVEADKTTEKYLEERIADFKLDPKWKSDDDALFAESKQYDVSDLSPQVA
                     CPHNVDNVKSVEEVEGTKIDQVFVGSCTNGRFEDIEIVAKMLNGEKVAKGVRLLIIPA
                     SHTEYMKTLRAGYVEQFMEAGAIVESPCCGPCMGGSFGLLGKGEVGLSTSNRNFKGRQ
                     GSPDAFVYLSSPATAAASALYGEIKDPRKV"
     gene            2177..4021
                     /locus_tag="VKL21_00495"
     CDS             2177..4021
                     /locus_tag="VKL21_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HMB44277.1"
                     /translation="MKEEFCIVPDTSVIIDGRITARLKAGEFKSARVIVPEALVSELE
                     AQANRGKEMGFKGLEELKELSDMAKRGAITLEFTGKRPSLEQIKLASGGEIDAMIRGI
                     AVENNALFITSDRVQSEVARAKGLNVEYLMPLMEELKVLGIEDYFTDDTLSVHLKVNV
                     PPCAKRGSVGKQKLVQIREEVSTEKELHTLTREFVERTKRDLDSHLEIEYEGVMVFQI
                     GSMRIAVAQRPFSDGMEITAVRPIAQVQLDDYRLSKELKARIIERQRGILVAGPPGAG
                     KSTFAASVAQVLYEQGFIVKTMESPRDLQVPDAITQYAPIQRDMEKTADILLLVRPDY
                     TIYDEVRKTKDFQVFADMRMAGVGMIGVVHATRGVDAIQRLIGRVELGIIPQVVDTVI
                     FIDKGEVSKVYDVIFTVKVPQGMVEQDLARPVITIADFETKAVEYEIYTYGEQVVVMP
                     VTDVERERPPTWGLAAREIEKELRKYTGNKINVEIVSDSSATVYMDEEDIAGVIGKGG
                     KNIDLIEKRLGIHLDIREKKKEKATIKGKGSGIKSFVPAIERSKKYVILTVKELSGAT
                     VDVYSGDEYLFTATVGRKGDVKIGKETETSFRVLENPDIITMRLAPGE"
BASE COUNT         1390 a          863 c         1031 g         1190 t
ORIGIN      
        1 tgatttatcg tttatagttt atgaatatga tatgggaaaa acccatctga tgcgcaaaga
       61 ttacattatc cattagaatc catccgatct gtgttcttgt cctggttttt tcatactctt
      121 tagaaaatga ccctggcaat aagtatatat ctttcatctt acatattgat gaaaagatga
      181 tgaatgttta aattctctga gcatgcaaga aaaagaatga tatcgagaga gataagcgaa
      241 gaggatgtga tagcagccat cgaaaatggg gaacttgaat tcacaagaat tgatgagaaa
      301 ggcagaggga cggaatacac gcattctatc gagctcaaag atccattttg gagaaaaata
      361 atggttggat ggacatatga tggagatgac ttcctgatac tcacgcttta tgaaatcaaa
      421 agaaaataga ggtataatga caactataat atgcggcaaa tgcaagcagg agatgcggga
      481 gattgaactt gaacagtacg aatttgaaag gggcatcgtc cttgataatg taagagccat
      541 gcgatgccca caggggcatg ttacatttac cgaggagcag gcgctaaaca tggagcaacg
      601 cactgaggat attaaaaagc atgcctttgg attcgttaga tctgtaagca aaagtgccag
      661 gtcacttgta ataagaatac ccgccgatct tgcaaagcat ttggatatta ccgagaatag
      721 caagattgaa atgatcccgc tgggtaaaaa gaaatttatg gttcaggtaa agtagttttt
      781 ggttttatcc cagtaatttc aaaaggatat caaacctttc tcggatcctt tatctcccca
      841 tacagcgcgc ttgccgctgc ggtcgcagga gatgagagat aaacaaaagc atcagggctt
      901 ccctgcctgc ccttgaaatt cctgttcgat gttgagagac ctacctcacc cttgcccaga
      961 agtccgaatg agccgcccat gcatggtccg cagcatggcg attcaacaat ggcgcctgct
     1021 tccatgaact gctctacata tccggcacgg agggtcttca tatattcagt atgcgatgcc
     1081 gggattatca aaaggcggac accttttgcc actttttcac cattcaacat ttttgccacg
     1141 atctcaatgt cctcaaagcg tccgttggta catgagccaa caaatacctg gtcgatcttt
     1201 gttccttcca cttcttcaac ggatttcaca ttatcaacgt tatgcggaca ggctacctgc
     1261 ggcgaaaggt cacttacatc gtattgtttc gattcagcaa aaagggcatc gtcatcgctc
     1321 ttccacttcg ggtcaagctt gaaatccgct atcctttcct ccaggtattt ttctgtcgtc
     1381 ttatcagctt ccacgatccc tgcttttcca cccatttcga tagccatgtt ggacatggtc
     1441 atccttccgg gaatgtccat tttcctgata gttgagccgc aaaattcaga agtcatataa
     1501 cgtgcgcctt ccactccgac atcaccgata agatgcaaaa tgacatcttt cgggtaaacg
     1561 cattttggca gcttcccttc aacttcaaac cgaaatgtct gaggcacacg gaaccacagc
     1621 tttccagttg caaaaacaaa tgccatatca gtacttccta tgcctgtgga aaaagcgcca
     1681 agtgcaccat aagcgaatgt atgagaatca gaacccacaa tgaggtcgcc cggtctgaca
     1741 tgtcccttct caggcatcac ctggtggcat accccttcaa aaacatcata attcaggatt
     1801 ccctgctctt ttgcaaactg gcgaaggaaa atgtggttcg cggcggcatt tagcgaatca
     1861 gcagggacct ggtggtcaaa aagaataact attttgttgg ggtcccagac tgtccggttc
     1921 ttcttgcctt tgactatttc cctgaaaccc tcaacggcaa gcggacccgt aatatcatgt
     1981 gtcatggcgc aatcaatatt tgcgaggaca aagtctcccg cttttacttt tttgctagtt
     2041 gctttactga atatcttttc tgaaattgtt tgtgccatga agccttattg atagcttgaa
     2101 attatttaat cttttggaga tagagttgca tgaatctgcc aacatttaaa gcggaaggag
     2161 tgtattttgt tataatatga aggaagaatt ttgtatagtt cctgatacga gtgttatcat
     2221 cgatgggcgg ataacagcca gattgaaagc aggagaattc aaatcagcca gggtcatagt
     2281 gccggaagct cttgtgtctg agctggaagc gcaggcaaat cgtggaaaag agatggggtt
     2341 caaaggatta gaagagctca aagaacttag cgatatggca aaaagagggg ctatcacact
     2401 tgaattcacg ggaaaacgac cctctcttga gcagataaaa cttgcatccg gaggagaaat
     2461 cgatgccatg ataagaggca tcgcggtaga gaacaatgcc ctatttatta caagcgacag
     2521 ggtgcaatct gaagtcgcgc gggctaaagg attaaatgtt gaatacctga tgcctctcat
     2581 ggaagaatta aaagttcttg gcattgagga ttatttcacg gacgatactc tttcggtaca
     2641 tcttaaggta aatgtcccgc cctgtgcaaa aagaggcagc gttggtaaac aaaagctggt
     2701 gcagatcagg gaagaggtta gtacggaaaa agagcttcat acactcacaa gagaatttgt
     2761 tgaaagaaca aaacgcgacc ttgactctca tcttgagata gaatacgaag gcgtcatggt
     2821 attccagata ggctcaatgc gaatagctgt agcccagaga ccattttcag acgggatgga
     2881 aataacagct gtaagaccca tagcacaggt ccagcttgat gattacaggc tcagcaagga
     2941 attaaaagcc aggatcatcg aacggcaaag gggcattctt gtggcaggcc ccccaggcgc
     3001 cgggaaatcc acatttgcag caagtgtggc gcaggtactt tacgagcagg gtttcattgt
     3061 caagacaatg gaatcgcctc gcgatctaca ggtgcctgat gcaataacac aatatgctcc
     3121 aatccagcgc gatatggaaa aaactgcgga tatcctgctt cttgtcaggc cggattatac
     3181 gatttatgat gaggtcagga aaacaaagga tttccaggta tttgcagaca tgagaatggc
     3241 tggtgttgga atgatcgggg tagtgcatgc cacaagggga gtggatgcga tccagagatt
     3301 gatagggcgt gttgaactgg ggataatacc ccaggttgta gatactgtga tcttcattga
     3361 taaaggagaa gtttccaaag tatatgatgt aatattcacc gttaaagttc cgcaagggat
     3421 ggttgaacag gaccttgcaa gacctgtgat aacaattgcg gattttgaga ccaaagcagt
     3481 tgagtatgaa atatacacat acggtgaaca ggtagttgtg atgcctgtga ccgatgttga
     3541 aagggaacgt ccgcccacat ggggacttgc tgccagggaa atagaaaaag aattgaggaa
     3601 atatactgga aataagatta atgtggagat agtatcggat tccagcgcca ctgtttacat
     3661 ggatgaagaa gatatagccg gcgtcatcgg gaaaggtggg aaaaatattg acctgatcga
     3721 gaaaaggctc ggtattcacc ttgatattcg tgagaagaag aaagaaaagg ctacaataaa
     3781 aggaaagggg agcggaatta agagttttgt acctgcgatc gagcgttcca agaaatatgt
     3841 tatacttacc gtcaaagaat tatcaggtgc aaccgtagat gtttatagcg gtgatgagta
     3901 tctttttacg gcgacggtag gaagaaaagg tgatgtgaag ataggaaaag agacagaaac
     3961 atcatttcgt gtcctggaaa atcctgatat cattactatg agattggctc ccggggagta
     4021 atctgaagat tattgaactt tattctggat tttaaaggtt cattgaacct ttttttttga
     4081 atattatata gcttccaaag aagttaaact ttaaaatttg gttattttta attgaaaaca
     4141 tattgtaact aatactagac aatatatagt aaaaataatc attattgtac tttaaaatca
     4201 aatttaagac gaaataaacc cataaaacta tcaatatgtt tatatcaaat cgtagtgata
     4261 cgaacaattg tgatattctg catatctgga aaaacgtaag agttaaatag attaattcgt
     4321 atgtgtacaa acggttcgta tacctacgaa ttaacgtggg ttgcaggaaa acctttttgc
     4381 tgcccactat gcagaaatca aataataaaa aaggagataa aaaatgaata acaaactgaa
     4441 atttgggcta ctgcttttgg cggtttctgc cctg
//
LOCUS       DATGBN010000025         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_390030, whole genome shotgun sequence.
ACCESSION   DATGBN010000025 DATGBN010000000
VERSION     DATGBN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390030"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VKL21_00500"
     CDS             complement(<1..237)
                     /locus_tag="VKL21_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44278.1"
                     /translation="MNTAQDKYCKEGLKLFSRLGGYKAKSGIDYLHFVLDETIDFSGS
                     RSGFFFTVDRTGDINLVCKLDSQESISGNVHEKAL"
     gene            complement(324..1319)
                     /locus_tag="VKL21_00505"
     CDS             complement(324..1319)
                     /locus_tag="VKL21_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB44279.1"
                     /translation="MLDKLSNINQWRKDFILETFVLFLSIRGRINFLQLARYGKHKEQ
                     RYRQQFEKQFDFLTFNKELVLSSGSGRFAIAFDPSYISKAGKKTPGVNWYWSGCANQS
                     KWGLEIGGLAAIDIDNHIAFHLEAVQTLKTDEQSLTNWYAGVISARKETLTAISKYMV
                     ADAWFSKRTFVDPIVSMGMHLISRLRDDADLRYISKEKPTGKKGRPRKYSGKIILNDI
                     DKDYFTFTSQDEESTVYSSEVYSKALKRIIRLVHVTYHNAKGKETCKLYFSTDVSMPA
                     TDILLYYRSRFQIEFLYRDGKQFTGLNDSQARSENKLHFQFAGPMWSLCPVFALI"
BASE COUNT          482 a          311 c          298 g          535 t
ORIGIN      
        1 taatgccttt tcatgaacat tgcctgaaat actttcctgt gaatccaatt tacaaacaag
       61 attgatatct cctgttctat caacagtgaa gaaaaagcca gacctgctcc cggaaaaatc
      121 tattgtttcg tcaagaacga aatgaagata atcaatcccg ctttttgcct tgtaaccccc
      181 aaggcgtgaa aatagtttta aaccctcttt acaatattta tcttgtgctg tattcatatc
      241 caatttttct actgttttcc tgtataaaat taaactaaat atagatgctt cttgacatct
      301 tttaaattta aaattgtaag aagctaaatt agtgcaaaaa ccgggcataa tgaccacatt
      361 gggccggcaa actggaagtg caatttattt tcacttcgtg cctgggaatc gttgagcccg
      421 gtaaattgtt ttccgtcacg gtagagaaat tctatctgga aacgactacg gtaataaagc
      481 agtatatctg tggcaggcat agacacatcg gtactgaaat agagtttgca ggtttctttt
      541 cctttcgcat tgtggtaagt aacatgaacc agtctaatta tccgcttgag ggcttttgaa
      601 taaacttcag atgaatacac tgtggattct tcatcttggg atgtaaatgt aaaataatct
      661 ttatcaatgt cattaaggat aattttacct gaatacttac gtggtctgcc ctttttgcct
      721 gtaggtttct ctttgctgat atatctcagg tcggcatcgt ctcttaacct gctgataagg
      781 tgcataccca tagaaactat aggatctaca aatgttcttt tagaaaacca tgcatcagct
      841 accatgtatt tggatattgc ggtcagggtt tcttttctgg cagatattac cccggcatac
      901 caattggtaa ggctttgctc atcggtttta agtgtttgta cagcttccag atgaaaagcg
      961 atatgattgt cgatatcaat agctgcaagg cctccaattt ccaagcccca tttggattga
     1021 ttggcacagc cagaccaata ccagttaact cctggtgttt tcttgcccgc cttgcttata
     1081 taacttggat caaaggcaat cgcaaaacgc ccactgcctg aggacaaaac aagttcctta
     1141 ttaaatgtta agaaatcaaa ttgtttttca aattgttgtc tgtacctttg ttctttatgc
     1201 ttgccgtagc gagccagttg taagaagtta atacggccac gaatggataa aaacaggaca
     1261 aaagtttcta agatgaaatc ttttcgccac tggttgatat ttgataattt gtctaacatg
     1321 ctaataataa gctggttata agttttatgt tccctggtaa tcataactgt caaagattgt
     1381 ggtaaatcct ttaagatact gattatcagg gacttaaact aatttttatt catttatttt
     1441 attgaaaatc aaacatttat attaaagttt aacgaaccat tgttataaag gaggctgtct
     1501 caaaacaaaa agaggcagcc tctctttttt ttaatctctt aagggttaat tccccgccgc
     1561 ttgcggcgaa tatgaaataa ttccagaaga taccccgctg cttgcggcgg ggagcttcat
     1621 tgagat
//
LOCUS       DATGBN010000026         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_545512, whole genome shotgun sequence.
ACCESSION   DATGBN010000026 DATGBN010000000
VERSION     DATGBN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545512"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1154)
                     /locus_tag="VKL21_00510"
     CDS             complement(138..1154)
                     /locus_tag="VKL21_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRM11 family methyltransferase"
                     /protein_id="HMB44280.1"
                     /translation="MLIAFELSGEHNTIPASEVLSCLRLLNADFWVCHSLDGCLVVDI
                     KARTGEIIENISKRVAMTHHIIKVLGMGGPDEHEILDMVEKNIPDFDGTYSVRVKRIR
                     DYSKINTELMEGEIGSIFHENGKHANLKNPQIQFRVVLTEDKSIFGCLLSSIDRSIFE
                     SRKPHYKPFFYPGVLMPRIARALVNISMPQEDLLDPFCGTGGILVEGGLIGIKVIGGD
                     MQRKILLGAKMNLEHYNVNYSLMYEDACRLALRNESVDAVVTDPPYGRSAAIKAESLE
                     YMLAISMKEIHRVLKNNKRAVIVSERPIEELAQDAGFKVIEKHLQRVHKSLTRRILVL
                     EKSC"
     gene            complement(1168..>1708)
                     /locus_tag="VKL21_00515"
     CDS             complement(1168..>1708)
                     /locus_tag="VKL21_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMB44281.1"
                     /translation="WARAQVPDDFDPRRDAKYREYMYIIPEKLDISILNSASGLIEGR
                     HDFSNFITPEKERSSSTIVYDLNIRTLGEFTIMEIRADHFLWHMVRKIATALKMIGNG
                     KRDGSWLEKMLAPCEFHEALEPAAANGLILKNVGYTDIDWKEDSYARKKVSQNLEEEF
                     LWHSVMAQILKELRKNMTL"
BASE COUNT          451 a          404 c          303 g          550 t
ORIGIN      
        1 ttcgtatata tacgaattgt agtgagcaga aattttttta gaaaaccacc ataaccattc
       61 tgctcactcc tccttttcca ggtcacttga tcaatgatca agaatatata taccagattt
      121 acaccgatat taaatatcta acatgatttt tcaagtacca ggatacgtcg ggtcaggctc
      181 ttatgcaccc tttgcaggtg cttttctatg accttgaagc ctgcatcctg cgcaagctct
      241 tcgatgggac gttcggacac aatgaccgca cgtttgttgt tctttaaaac tcgatgtatc
      301 tctttcatgg atatggcaag catatattcg agagattctg ctttaattgc agctgatctc
      361 ccgtagggcg ggtcagtaac cacagcatcc acactttcat tgcgaagcgc aaggcggcat
      421 gcatcttcat acattaagga atagttaaca ttataatgtt caagattcat tttggcgcca
      481 agcaggatct ttctttgcat gtctccacct atcactttta ttccaatgag tcctccttct
      541 acaagaattc ctcctgttcc gcaaaacggg tccaggagat cttcctgcgg cattgaaata
      601 ttcacaagcg ctcttgctat tcttggcatc aggaccccgg gataaaaaaa aggcttataa
      661 tgtggttttc gcgattcgaa tatactcctg tcaatcgagg acaaaagaca tccgaaaatg
      721 ctcttatctt ctgtaaggac aaccctgaac tgaatttgtg gatttttcag gtttgcatgt
      781 tttccatttt catgaaaaat acttcctatt tccccctcca tcaattctgt gttgatctta
      841 gaataatctc ttatgcgctt gacccgaacg ctatatgttc catcaaaatc cggaatattt
      901 ttttcaacca tatccagtat ttcatgttca tccggtcctc ccataccaag gaccttgatg
      961 atgtgatgtg tcattgcaac ccgtttagaa atgttttcga ttatttcccc tgtcctggct
     1021 tttatatcga caacaagaca cccgtcaagt gaatgacaaa cccaaaaatc tgcatttaac
     1081 aacctgaggc acgacaaaac ttctgatgcc ggaatggtat tatgttcccc tgaaagttca
     1141 aatgctatca acatattaat tttcctgtca tagtgtcatg tttttcctga gttcttttag
     1201 tatttgcgcc attacactat gccataagaa ttcctcctca agattttgcg atactttctt
     1261 tcttgcataa gaatcctctt tccaatcaat atcggtgtat cccacgttct tcaggatcaa
     1321 accattagcc gcagcgggtt ccagcgcttc atggaattcg caaggggcaa gcatcttttc
     1381 aagccatgaa ccatcccttt tcccgtttcc tatcattttc aatgcggttg caatctttcg
     1441 gaccatgtgc caaagaaaat ggtccgctct gatctccatg atcgtgaact cgccgagtgt
     1501 gcggatattc agatcatata cgattgttga actgcttcgt tctttctcag gcgtgataaa
     1561 atttgaaaaa tcgtgcctgc cttctattaa acctgatgcg ctatttagaa tagaaatatc
     1621 caatttttca ggtatgatat acatatattc cctgtatttt gcatcgcggc gcgggtcaaa
     1681 atcatcaggg acctgggctc ttgcccat
//
LOCUS       DATGBN010000027         6761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_351634, whole genome shotgun sequence.
ACCESSION   DATGBN010000027 DATGBN010000000
VERSION     DATGBN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351634"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VKL21_00520"
     CDS             <1..646
                     /locus_tag="VKL21_00520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HMB44282.1"
                     /translation="TKNRGHSISRTLLLNNEKLPDISDFDWLVIMGGSMNVYEEDKFP
                     WLTEEKHFIAKAIEANKILLGVCLGSQLIADVLGGKVSKSQYKEIGWFPVSLTREARD
                     SSIFGGLPGTFTAFHWHGDTFKIPQGAKRMAQSEGCMNQAFECGRAIGLQFHLEYSKR
                     SIDLMFQNCSDELVEGKYIQTPDEILTKIANVYEMNKTLDLILDNMEREFGIRL"
     gene            653..1027
                     /locus_tag="VKL21_00525"
     CDS             653..1027
                     /locus_tag="VKL21_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HMB44283.1"
                     /translation="MEDTFFLAILIFAAGLLYSSVGHAGASGYLAAMALFGLAPDVMK
                     PTALILNILVAIIATGHFFRAGYFSWKIFWPFAVGSIPFAFIGGALSLPGSVLQASPW
                     PDPVICSISAISIQALRSRRCG"
     gene            1018..1182
                     /locus_tag="VKL21_00530"
     CDS             1018..1182
                     /locus_tag="VKL21_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44284.1"
                     /translation="MRLSRFLLVYTWAIAAMLGGIIGSGLGSRRFVNATLYRLLSVVL
                     VIAGAKLMLA"
     gene            1394..1585
                     /locus_tag="VKL21_00535"
     CDS             1394..1585
                     /locus_tag="VKL21_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44285.1"
                     /translation="MLESSGFLTQKDIVKQTYLPARTVRYALNRLKEEKVLQERFYFQ
                     DARQSLYGLNGSVPGVVTG"
     gene            1796..2926
                     /locus_tag="VKL21_00540"
     CDS             1796..2926
                     /locus_tag="VKL21_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HMB44286.1"
                     /translation="MKFINHKPGDIEICDVTLRDGEQTPGVAFTTQEKIAIAQKLDSI
                     GVDVIEAGFPVVSHAEEATVREIAHLGLDAKICCLARSVAKDVDTALRCDVDFVSIFI
                     ATSDLHLKYKYHKTFEEATSCALDVLDYAKDHGLKVRFAVEDATRTDIRVLKSIFKSA
                     EEHGADYVSIADTVGILNPSTTFYLVSEIKKTVKTRLCIHCHNDLGLAVANTLSGAEA
                     GAFQLHTTINGIGERCGNAALEELLVSLRVQYGIEKYDVSKLMELSKLVEQYTEIPIP
                     KTKAIVGANAFAHESGIHVAAVLEEPMTYELFAPEMVGAKREIIIGKHTGSKALKSVV
                     QKMGYDLSHEQMRTLLDKVKKCSEAKKKVSCGRLTEFIKELE"
     gene            complement(2984..3469)
                     /locus_tag="VKL21_00545"
     CDS             complement(2984..3469)
                     /locus_tag="VKL21_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HMB44287.1"
                     /translation="MECEICGTDIKGNPIRVTVEGTILDVCSKCAHYGKPQDKWTPVS
                     RKMAPTERVIVTHRPKRDAFDKLEDEILPDYAQVIRKARESQGLTIEKLASKMMEKAT
                     LIRKIEREELVPEDTVRKKLETTLNIKLTERVSSQDQRGGGFIRGTTLGDVAIIRKKG
                     K"
     gene            3522..4763
                     /locus_tag="VKL21_00550"
     CDS             3522..4763
                     /locus_tag="VKL21_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HMB44288.1"
                     /translation="MTESQDNSQESPVSMGNNDFSRYLMDRIKMLEERNLLLKEQANK
                     IENEKRFTESQMLNYEREIRQLKSELERIKTPPLVVGTVVDVLDGGKIIIRSSTGPQF
                     VVGSTQFINNSDLVPGTAVSLNYQSLAVVGILPSSRDPFVHGMEVIESPNVSYSDIGG
                     LEEPIRELRETVELPLTKPEVFERIGIEPPKGILLFGAPGTGKSLLAKAVASQTKATF
                     IRIVGSELVQKYIGEGARLVRDLFKMAKEKSPSIIFIDELDSIGAKRIDSATSGDREV
                     QRTLMQLLSEMDGFNPRGNVRIIAATNRPDILDLALLRPGRFDRFISIPMPNKDARKM
                     ILKIHSRKMKLSEEVDFDELSSLTDGANGSDLKAITMEAGMFAVREERFGVGMDDFRN
                     AIRKLLIAPTKHSLHSEGMFA"
     gene            4779..5147
                     /locus_tag="VKL21_00555"
     CDS             4779..5147
                     /locus_tag="VKL21_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMB44289.1"
                     /translation="METIANMLEKILDEILSVGGVELCAIVSKQGLLMVSREANPGLD
                     SEAFAALTATLYMSAESTTMRLSKQKPKSIIVETNDKQLITYEAGPDALIVVLVANKG
                     FIGLILNQLKKAAEKIKNVI"
     gene            5210..>6761
                     /locus_tag="VKL21_00560"
     CDS             5210..>6761
                     /locus_tag="VKL21_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMB44290.1"
                     /translation="MKEDKRKPKKVSQLRKRAEDALQGRTEGLQELSIRDARSLIHEL
                     QVHQIELEMQNEELRMAQKELEESRDRYSDLYDFAPVGYFSLDKKGLIIEVNLTGAKK
                     LGVERNFLIKKPFSLFISPGSKDVFYLHLRQVFSTGVPQTCELKLVDKKGVQLVVQLE
                     SLSAQEENSSNCRTAMSDITDRKIAEEAVRNTLAYTESIVDTIREPLVILDAHHRMKT
                     ANHAFYKTFKIPIENTIGKSVYELGNNQWNIPRLRELLEKIIPRNTQFRDFEIEQEFP
                     NIGRKAMLLNACRLKLAGTDMILLAIEDITERKKIEELRLENERLISASKARSEFLTI
                     MSHELRTPLTSVIGYSIILKGNTRGELNEKQRFFVDNILKSSKHLLDLINNFLELAKT
                     EAGKQEMVFEEISVQDAINEIFELVKENAIGNNIILRKEFDPEMLFIQADRRALKQML
                     FNLLSNAIKFNKKEGGTITVSAKRENDMVKISVDDTGIGIKEEDLPRLFRKFEQLDSG
                     ISRKYEGTG"
BASE COUNT         2024 a         1376 c         1595 g         1766 t
ORIGIN      
        1 gacaaagaac aggggacata gcatttcaag aacccttctc ttaaacaacg aaaaactccc
       61 cgatatcagc gactttgact ggcttgtcat tatgggcggt tcaatgaacg tctacgagga
      121 agataagttt ccctggttaa cagaagaaaa gcattttatt gcgaaagcta ttgaagcaaa
      181 taaaatcctt ctcggagtct gtcttggttc ccagttgata gcagatgtcc ttggaggcaa
      241 ggtcagcaag agccaataca aggagattgg ctggtttccc gtttcattaa ccagagaagc
      301 aagagactct tcgatatttg gcggccttcc gggcacattt actgcgtttc actggcatgg
      361 cgacacattc aaaataccac agggcgcaaa acgaatggca caaagtgaag gatgcatgaa
      421 ccaggcgttt gaatgtgggc gcgcaatagg attgcagttc catcttgaat actcaaaaag
      481 gagcatcgat ctcatgttcc agaattgcag tgatgagctt gttgagggga aatacattca
      541 gacgccagat gaaattctta caaagatcgc aaatgtctat gaaatgaata aaacattgga
      601 tttgatactg gataacatgg aacgtgagtt cgggattcga ctgtaatttg atatggaaga
      661 tacctttttt cttgcaatat tgatatttgc agcaggattg ctctattcct cggtgggtca
      721 tgccggagct tctgggtatc tggcagcaat ggcattattc ggtcttgctc ctgatgtaat
      781 gaagccaacc gcgcttatac tgaatattct tgtggcgatc atagccacag ggcatttctt
      841 ccgggctggg tatttctcgt ggaagatctt ctggccgttt gctgttggtt ctatcccatt
      901 cgcctttatc ggaggtgctt tatctcttcc gggttctgtt ctacaggcaa gtccttggcc
      961 tgatcctgtt atttgcagca tatcggctat ttcaattcag gcactcagaa gccgcaggtg
     1021 cggttaagcc ggttcctgtt agtctataca tgggcaattg ccgcaatgct tggcggaata
     1081 ataggctccg gcctgggcag ccggcgattt gtaaatgcaa ccctttaccg tctgctttca
     1141 gttgtccttg taattgctgg cgcaaagctc atgctggcgt gactttccgc cgtgattttt
     1201 tcctgaacaa ccaaaatcag aagaattgtg acaataattg ctccaataaa tagatttata
     1261 aaggataaat cagagtttaa gccggagaat ttaaaaatga attttagaag gaatcgtctt
     1321 tgccaacaat ggcccacaaa ttgaactgat aaagatagat catttaccgc catcagcaaa
     1381 acttgttttt aaagtgcttg aatcgagcgg ctttttaaca cagaaagata ttgtgaagca
     1441 aacctatctt ccagccagga cagtgcgtta tgccctgaac aggctgaaag aagaaaaagt
     1501 attgcaggag cgcttctatt tccaggatgc gcgccagagc ctgtacgggc tgaacggcag
     1561 tgttccgggg gtggttacag gttgactaac tcttgttttc ttaatattag ccacaaataa
     1621 gccgaaactt cggctccggg aaggttcaag aagcgcaatg catgaacccc tccggaggct
     1681 tcttttattt aatattcttg aaatacattt cagcaaaaaa agcaaagata taagtaatga
     1741 ttaaacgtcg ttaataatct tgataaatat ggctaattat tcaaaaaaca atctaatgaa
     1801 attcatcaat cacaaacccg gtgatataga gatatgtgat gtcactttgc gggatggcga
     1861 acagacgcct ggtgtggctt tcacgacaca ggaaaaaatc gcgatcgcac aaaaacttga
     1921 tagtatcggt gtcgatgtca tagaggcggg attccctgtt gtatcgcatg cagaagaagc
     1981 tactgtaaga gaaatcgcgc atctggggct tgatgcaaag atatgctgtc ttgcacggtc
     2041 agtggcaaaa gatgttgaca cggcgttaag atgcgatgtg gattttgtaa gcatcttcat
     2101 agcaacatca gacctgcatc tgaaatacaa ataccataag acatttgagg aggctacatc
     2161 ctgcgcgctt gatgtgcttg attatgcaaa ggaccacggt cttaaagtga ggtttgcggt
     2221 tgaagatgca acccgcacgg atatcagggt tctaaagtct attttcaagt ccgcggaaga
     2281 acacggcgcg gattatgtca gtatcgccga tacggtgggg atattgaatc ccagtacaac
     2341 attctacctg gtcagcgaga tcaagaaaac tgtaaaaacc aggttgtgca tccattgcca
     2401 taacgacctt ggattggctg ttgcaaatac gctaagtggc gccgaggcag gagctttcca
     2461 gctgcatact acgattaacg gcattggaga acgctgcgga aatgctgctc ttgaggaact
     2521 tcttgtgagc ctgcgcgttc aatatggtat tgaaaagtat gatgtaagca aattgatgga
     2581 gctatcaaag ctcgtggaac aatatacgga aatacccatc cccaagacaa aagccattgt
     2641 aggagcaaat gcatttgccc acgagtcggg gatacatgtg gctgccgttc ttgaggaacc
     2701 tatgacctat gaattattcg cccctgaaat ggtgggcgca aaacgggaaa taatcatcgg
     2761 gaaacacaca ggttcaaagg cattgaaaag tgtagtccaa aaaatgggat atgatctctc
     2821 acacgagcag atgcgcacgc tcctggataa agtcaaaaag tgcagtgaag ccaagaagaa
     2881 agtttcatgc ggacggctta ctgagttcat aaaagaactt gagtaacaac acggttggag
     2941 agggtagatt ctttatttca ttgatatgaa cttgtacaaa tccctatttc cctttcttcc
     3001 tgataattgc cacatctccg agggtggttc ccctgatgaa accgccgccc cgctggtcct
     3061 gcgaagatac cctctcggtc aatttgatat ttaaagtagt ttcaagcttc ttgcggactg
     3121 tatcctcagg caccagttcc tcccgttcaa tttttctgat aagagtggct ttttccatca
     3181 tctttgatgc cagtttctca atggtcaatc cctgggattc cctcgctttc ctgataacct
     3241 gggcataatc cgggaggatc tcatcctcaa gcttatcaaa agcatctctt tttggcctgt
     3301 gtgtaactat taccctttct gtcggggcca tttttcttga cactggagtc catttgtcct
     3361 gtggctttcc atagtgggcg catttgctgc acacatccag aatagtccct tcgactgtta
     3421 cacgaatagg atttcccttt atatccgtac cgcaaatttc acattccatc acatcatctc
     3481 gacgaaaact ctatataccg tccacgctta aatatcattc gatgacagaa tcccaggata
     3541 attcccagga aagcccggtc tcaatgggga acaatgattt ttcacgctat ctgatggaca
     3601 ggataaaaat gcttgaggaa aggaatcttc tcctcaagga acaggccaat aagatagaaa
     3661 atgaaaagcg ttttactgag agccagatgc ttaactacga aagagagata cgccagctga
     3721 aaagtgaact tgaacgaata aaaacacctc cccttgttgt gggtactgtt gtggatgttc
     3781 ttgatggcgg gaaaataata atcagaagca gtaccggccc ccagttcgtt gtgggatcaa
     3841 cgcagtttat caacaactca gacctggtgc ctggaactgc ggtttcttta aattaccaga
     3901 gtcttgccgt tgtgggtatt cttccatctt cgcgcgaccc gttcgtacac gggatggagg
     3961 tcatcgaatc acccaacgtt tcatattcgg atatcggggg gcttgaagaa cccatccgtg
     4021 agcttcggga gactgttgaa ctacccctta ctaaaccgga ggtattcgaa cggatcggaa
     4081 tcgagcctcc aaaaggtata ttattatttg gcgctcccgg tactggaaaa tccctccttg
     4141 caaaagccgt tgcaagccag acaaaagcaa catttatcag gatcgtgggt tcggaacttg
     4201 tgcaaaaata tatcggtgaa ggagcccggc ttgtacggga ccttttcaaa atggccaaag
     4261 aaaaatcccc gagtattatc ttcatagacg agcttgattc cataggggca aaaagaatcg
     4321 attcggctac atccggagac cgtgaagtcc agcgcacatt gatgcagctg ctttcggaaa
     4381 tggatggttt taatcccagg gggaatgtca ggatcatcgc tgcaacgaac aggccggata
     4441 ttcttgatct tgctcttctt cggccgggaa gatttgaccg gtttatctca attcctatgc
     4501 caaacaagga tgcacgaaag atgatcctga aaatccattc caggaaaatg aagctttcgg
     4561 aggaagttga ttttgatgag ctgtcctccc ttaccgacgg ggctaacggt tcagatctaa
     4621 aggcgatcac aatggaagcg ggaatgttcg cggtaagaga ggaacgtttt ggcgttggaa
     4681 tggatgattt ccgtaacgcg attagaaagt tattgatcgc ccccacaaag cacagtcttc
     4741 attcagaggg tatgttcgct tgattcgctg aggaaataat ggaaacaatc gcgaatatgc
     4801 ttgaaaaaat ccttgacgaa attctatcag tcgggggtgt ggaactttgc gccattgttt
     4861 caaaacaggg attactgatg gtttccaggg aagcgaatcc cggacttgat agtgaagcat
     4921 tcgctgcttt gacggctacc ttatatatgt cggctgaatc tacgactatg agattgagta
     4981 aacagaaacc taaatcgatc attgttgaaa caaatgataa gcagcttata acatacgaag
     5041 ccggtcctga tgcgttgata gttgtcctgg tggcgaataa gggctttatc ggtctcatcc
     5101 tgaaccagct aaaaaaagct gcagaaaaaa ttaagaatgt catttaacct cgtgatgtca
     5161 aggagtatga aaaaaacccg aatgagccaa taaaggataa atcccaggtg tgaaagaaga
     5221 taaaagaaaa ccaaaaaaag tatcgcagct gcgaaaacgg gccgaagatg cgctgcaagg
     5281 aagaactgag ggactgcaag agctatctat cagagatgcc cggagtctga tccatgaact
     5341 tcaggtgcac cagatagaac tggaaatgca gaatgaagaa cttcgcatgg cacaaaaaga
     5401 gctcgaagag tcgcgtgaca ggtattcgga cctgtatgat tttgcccctg ttggttattt
     5461 ttctttggat aaaaaagggc ttattataga agtaaatctg accggcgcta aaaaattagg
     5521 cgtagaaaga aactttttga tcaagaaacc attttcgctt tttatttccc cgggtagtaa
     5581 ggatgtgttt tacctgcact taaggcaggt tttcagtacc ggcgttccac agacatgcga
     5641 gctcaaactt gtggataaga agggagttca gttagttgtt caattggaaa gcctatcagc
     5701 acaggaggag aattccagca attgcaggac agcgatgagc gatattacgg atcgcaagat
     5761 agctgaggaa gccgttcgga atactctggc atataccgag agtatcgtag atacgatacg
     5821 ggaacccctt gtcattcttg atgcacacca tcgaatgaag acggcgaatc atgcttttta
     5881 taagactttc aagatcccga tcgaaaatac tataggcaaa tcggtatatg aacttgggaa
     5941 caaccagtgg aatattcccc ggttgagaga attacttgag aaaatcattc ccaggaatac
     6001 ccagttccgg gattttgaaa ttgagcagga gtttccgaat atagggcgaa aagcgatgtt
     6061 actcaatgcc tgccgactca aactggcagg tactgatatg atactcctcg cgattgaaga
     6121 tattactgaa agaaaaaaaa tagaagaatt gcgtcttgag aatgaacgtc tcatcagcgc
     6181 aagcaaagca agatcagaat ttttgacgat catgagccat gaactgcgaa ctcctctaac
     6241 atctgttatc ggatattcaa taattttgaa ggggaatacc cgtggtgaat tgaatgaaaa
     6301 gcagaggttc ttcgtggata acattcttaa aagcagcaaa cacttgcttg atcttattaa
     6361 taattttctc gaacttgcca aaacagaagc aggaaagcag gaaatggtct ttgaggaaat
     6421 atctgttcaa gatgcaataa atgagatatt cgagctggtc aaagagaatg ctatagggaa
     6481 taatataatc ctgagaaaag aatttgaccc ggaaatgctg tttatacagg ctgatagaag
     6541 ggcgctcaag cagatgctat ttaatttgct gagcaacgca ataaaattca ataaaaaaga
     6601 aggagggacc attaccgtat ccgcaaaaag agaaaatgat atggtgaaaa tatcagttga
     6661 tgatactggt attggaataa aggaagagga tttgccaaga cttttccgga agtttgagca
     6721 acttgattca ggaatatcca gaaaatatga agggacaggc c
//
LOCUS       DATGBN010000028         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_468060, whole genome shotgun sequence.
ACCESSION   DATGBN010000028 DATGBN010000000
VERSION     DATGBN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468060"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VKL21_00565"
     CDS             complement(<1..304)
                     /locus_tag="VKL21_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HMB44291.1"
                     /translation="MLSKNDYKGIIKSMGLVFGDIGTSPIYTLTVIFLLTPPTLAHVM
                     GILSLIIWTLIIVVTVEYAWLAMSLGQKGEGGTIVLQQHIIPLLKSGRKISFVTLLT"
     gene            complement(413..1549)
                     /gene="sat"
                     /locus_tag="VKL21_00570"
     CDS             complement(413..1549)
                     /gene="sat"
                     /locus_tag="VKL21_00570"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HMB44292.1"
                     /translation="MTQIKPHGGKLINRTLTEQKRTKIQEQASEYQTVEVSDDLMKDI
                     ENIASGLFSPLEGFNCLEDYESILYNKRLSNGLPWTLPIVLDTNNKNIKEGDEILLKN
                     DNNLVAMMQVEEIFGYEKRAYAEQVYGTNDAAHPGVAKTYSMNETLLGGKINLINESE
                     TPYFKYAMKPQETRNLFKEKGWEKVVGFQTRNVAHLGHEYLQKSALTMVDGLFINPVI
                     GKKKKGDFKDDVILESYEVLIENYYPRDRVVLGIFQTEMRYAGPREAIFHAIVRKNYG
                     CTHFIVGRDHAGVGSYYHPFAAQEIFKEFPDIGIEPMFFMSFFHCNKCGGISNDKVCP
                     HSDRVDFSGTKMRQMIEAGKRPPADSMRPEVADVILKSGKPFVE"
     gene            1600..1911
                     /locus_tag="VKL21_00575"
     CDS             1600..1911
                     /locus_tag="VKL21_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44293.1"
                     /translation="MIDQAKGAIKYYVGIAAIYSAIFALMLGFAGSFFGSMMGFGNLG
                     MMMGAGVGVSAAIMIFAIAGAFIGGAILHIFVYIAGGRKGRIQTIKAGMYGSTAPLKV
                     E"
     gene            2215..2448
                     /locus_tag="VKL21_00580"
     CDS             2215..2448
                     /locus_tag="VKL21_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44294.1"
                     /translation="MKTITIRLEDDVFNKIDEQRGTILKSDFYRELIEYHLNKSESDL
                     NTIEYKKLESEYEKLNSEYKAFKTELQHTEAIR"
     gene            2668..>2943
                     /locus_tag="VKL21_00585"
     CDS             2668..>2943
                     /locus_tag="VKL21_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44295.1"
                     /translation="MKSKTPSFVLELAMKTTSLQETAIVTRLEAGRHLYNACLGEALK
                     RLDLIRQSIEFKKIIELPAGKERTEKFRNLNGKYGFTEYALHDYAGAI"
BASE COUNT          869 a          585 c          617 g          872 t
ORIGIN      
        1 aagtaagcaa agtgacaaat gatatcttcc tgccggattt cagcaatggg attatatgtt
       61 gctggagaac aatggtccct ccttcacctt tctggccaag gctcattgca agccatgcat
      121 actcaacagt caccacaata atcagggtcc agataataag ggaaagaatt cccataacat
      181 gggctaatgt gggaggagtc aatagaaaaa ttaccgtaag agtatatatt gggcttgttc
      241 cgatatcccc gaaaaccagt cccatcgatt ttatgattcc cttatagtca tttttagaga
      301 gcatgattaa tccatttcaa gattgattta ttctggagat ttcattcaaa ggttttaata
      361 ctttccaata gccaataaaa agagaaaaag atatatttcg aataaagaat ggtcactcca
      421 caaacggttt tcctgacttc aatatcacat cagccacttc gggtctcatg gaatccgctg
      481 gcggtctctt acctgcttct atcatctgtc tcatctttgt accggaaaaa tccaccctgt
      541 cagagtgcgg acagaccttg tcgttggata tgccgccgca cttgttgcaa tggaagaatg
      601 acatgaagaa catgggctca attccgatat cagggaactc cttgaatatt tcttgtgcag
      661 caaacggatg atagtagctt cccacccctg catgatctct tccaacaatg aaatgagtgc
      721 agccgtaatt cttcctcaca atggcatgga atatggcttc cctggggcct gcgtagcgca
      781 tttctgtctg gaaaatgcca agcacgaccc tgtccctggg ataataattt tctatcagga
      841 cttcatagct ttcgagtatt acatcgtcct tgaaatcacc ctttttcttc ttaccgataa
      901 caggatttat gaacagacca tccaccatcg taagcgcgga tttttgcaga tattcatgac
      961 caagatgcgc aacgtttcgt gtctggaacc ccactacttt ttcccagcct ttttccttga
     1021 agagattcct ggtttcctga ggcttcatcg catatttgaa atagggggtt tccgattcat
     1081 ttatcaggtt tatctttcct ccaagtaacg tttcattcat ggaataggtc tttgccacac
     1141 ccggatgggc ggcatcgttt gtcccatata cctgctctgc gtatgctctt ttttcgtagc
     1201 cgaagatttc ttccacctgc atcatggcta cgagattatt atcattttta aggagaattt
     1261 catctccttc tttgatattc ttgttattgg tatcaagcac gatgggaaga gtccatggaa
     1321 ggccgtttga aaggcgcttg ttatagagta tgctttcata atcttccagg cagttgaaac
     1381 cttcaagagg gctgaatagt ccggaagcga tattttcaat atctttcatg aggtcatcgc
     1441 tgacctcaac agtctggtat tcagaagcct gctcctggat ctttgttcgt ttttgttcag
     1501 tcaaagtcct gttgattaat tttccgccgt gaggttttat ttgtgtcatt gtaatcccag
     1561 attttggcct tcattgtaat tgctgcgtaa taattgtttg tgattgatca agccaaagga
     1621 gccataaaat attatgtggg tattgctgcg atatattctg cgatttttgc gttgatgtta
     1681 gggtttgcag gtagcttttt tggttcaatg atgggattcg ggaacctggg aatgatgatg
     1741 ggcgcgggtg taggggtaag cgctgcaata atgatatttg cgatcgctgg tgcattcata
     1801 ggcggggcga tacttcatat tttcgtatat atagcggggg gaagaaaagg gaggatccag
     1861 acaatcaagg caggcatgta tggatctact gcgccactga aagtggagtg aatcccttcc
     1921 agtagaaaaa aggattatat attttcttta gggtggccag cgcgccaccc ttgcctgaat
     1981 tggagaacaa cctatccatt tatccaatac tcaaaatgta ttttaataca tatatagatt
     2041 atctaagatt tttatgctca gtataattgt gattatgagc aggcattctt tttaatgtca
     2101 ctcccacaca actatccaac gcctgctcta tccaaacaat tgttttggct ttttcaagat
     2161 gcttatcaaa ggatgataga aattatatat gtttgaaaac aacttgaatg aggaatgaag
     2221 acaataacga taagacttga agacgatgta ttcaacaaaa tcgatgagca aaggggcaca
     2281 atattaaaga gtgattttta tcgcgaatta attgaatacc acctgaacaa atctgaatct
     2341 gatttgaata cgattgaata caagaaactt gaaagtgaat acgaaaaact aaactctgaa
     2401 tacaaagcat ttaaaaccga attgcagcac acagaagcaa tacgataaat tcaggaagaa
     2461 aggataaagg atcttcaaaa ccaggtgggt tttttgcagc ttgaatatca aaaagtgagt
     2521 gataggttac agaaatgggt tcaaaggggg gagaaagctc ccgccttcag tcgtggagat
     2581 gaatttcccc tttgcaataa tacggaatac tttcgcatcg ctaagccaag acttaagacc
     2641 aaaactatcc atacaacaat ttgaactatg aaaagcaaaa ctccttcttt cgtgctggaa
     2701 ctggcaatga aaaccacctc attacaggag actgccattg taacacgact ggaagctgga
     2761 aggcatttgt ataatgcctg tcttggagaa gctctgaaaa gacttgacct tatcaggcag
     2821 tccatagagt tcaagaaaat catagaattg ccagcaggta aagaaagaac cgaaaaattc
     2881 aggaatttga atggaaaata tggtttcaca gagtatgctc tccatgatta tgccggtgcg
     2941 ata
//
LOCUS       DATGBN010000029         8133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_40923, whole genome shotgun sequence.
ACCESSION   DATGBN010000029 DATGBN010000000
VERSION     DATGBN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40923"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VKL21_00590"
     CDS             <1..365
                     /locus_tag="VKL21_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44296.1"
                     /translation="PYYTFNTKGKEETIDYRVPIARIEQERKEEARFLPGLARTDEPV
                     FNVPRLGKSNLRSWQDHEVIMILEDGRRVYRFYPWESKYALVEPYNYTDVSIYDYLKR
                     LKDDKENIDDYSSIWHYF"
     gene            417..608
                     /locus_tag="VKL21_00595"
     CDS             417..608
                     /locus_tag="VKL21_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HMB44297.1"
                     /translation="MKKYKVICITSGSTVPPEPDRIEREITAMTEQGWDFLQITSGGG
                     GTGSGNITSWIYLIFKRES"
     gene            620..1378
                     /locus_tag="VKL21_00600"
     CDS             620..1378
                     /locus_tag="VKL21_00600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB44298.1"
                     /translation="MIKKNKRPSLENIIESEDVCLEILHPGGLDITRELSQLCHIGKA
                     IKVLDVGSGKGESAFYLVRNFNCHIIGIDISDHMIKRAKRKAIDQGVIIQFKKGDAHD
                     LPFKDNTFDAVISECTTSILDKERALGEMVRVARSGGYIGIHDICWKKGTPERQKQKL
                     TEIEFRRPETLEGWKTQFEKAGLIDVMIEDRSNLIPEWEKYILEKLGRIARFMIFLKI
                     IKNWGIGGLRMIMESRRIFQSQNTGYGMIVGKKP"
     gene            1375..2457
                     /gene="corA"
                     /locus_tag="VKL21_00605"
     CDS             1375..2457
                     /gene="corA"
                     /locus_tag="VKL21_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMB44299.1"
                     /translation="MSPGTRQNRMLKKRSTKTGLPPGALVHIGEKKAEKVRINILDYD
                     EYQFEEKEAKNIEECFPFKDKPTITWINIDGLHQVDIIEKIGKNFDLHPLLLEDILNT
                     EQRPKIEDFETYIYIVLKMLLYDEKTNEINSEQVSIIFGQNFVISFQEKEVDVFNPLR
                     EIIRTGKGRIRKMKADYLAYSLIDAIVDGYFLILEKLGENIEDVEEIMMANPSPETLK
                     AIHKLKRRMISLRKSVWPLREVVSALERSESSLIHDQTRIYLKDVYDHTIQVIDTVET
                     HRDMLSGMLDVYLSSISNKLNEIMKVLTMIATIFIPLTFIVGVYGMNFEFMPGLKWYW
                     GYPAIWGVMLMIVISMLLFFRKKKWI"
     gene            2495..3685
                     /locus_tag="VKL21_00610"
     CDS             2495..3685
                     /locus_tag="VKL21_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44300.1"
                     /translation="MRFLYGDSTEFDLQIDFLRLLNNFVDTGVRTIKYENAIFDLKNT
                     IIEKKRYNASVIEDMNGFVLNVETAISGAVASSKEQETLIKYAEKSKEYFNKFIEESK
                     TKFSDEILKEIAQLEKKIKEACEENRKILESFFIYDPIPIVDEKYTIRATEKGYSAQL
                     KIDCESGISCLFHIASSESPFSRNHVRARDFVKDVQIPARMKKPFLKKELVPDIVNIN
                     DFIIVDLVLSDKELEVVFRKRLDIKAEAFRLRMKFEKEFSVEVSEKEENGTQRSIDDI
                     PELKNELNYLRLQELGLKIIEKTKYLYPKKQKLETILLEEKDVFDENFIFELMQKVAE
                     IFAPTIAEIKKHSPSGEELSLKSEDEHGTRQEIYLKRSEIKDKLDEIGDKGKTLSEFL
                     NIII"
     gene            3735..3983
                     /locus_tag="VKL21_00615"
     CDS             3735..3983
                     /locus_tag="VKL21_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44301.1"
                     /translation="MVRLEREGNSFVFITGKTQPAQDINILLNALSELQNSAPDTSKI
                     KEGLLFIDNSNEFDIRNEIKNLLRKALESEGISLQDIS"
     gene            3994..5058
                     /locus_tag="VKL21_00620"
     CDS             3994..5058
                     /locus_tag="VKL21_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44302.1"
                     /translation="MPKLFLIPTINVLEVHDAVMRDWLSGLEDTAIKEHLEYPFKLER
                     FFPESARLALINGNKVHDEILALYNIIDPKAVFLNISTDLYELENKYNKRLISPHEFW
                     REYYENISKDISEPARTLYKIYLNEIIDKNTRLIESKERLPLNILFYGINLRSRDQLI
                     PVYEKASDMVEEFTLHIARAVNEIMNLRERPKHLWHSSLNATRVGISYEETEKFYDEL
                     LSRLKRLLKYKVREYFVKILWEQARAYVESYEEFLDHKIIEDENKFSNIADGLKILEL
                     QSEISGIVLLCEPGDYTVILEFLKNNMDHRFSIAQISLIRLFEKMRPFIQKNRIMQNN
                     YEIALNILGREKPKRFTNKL"
     gene            complement(5042..6337)
                     /locus_tag="VKL21_00625"
     CDS             complement(5042..6337)
                     /locus_tag="VKL21_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HMB44303.1"
                     /translation="MLELVPQFFNNMDVNFNFFEKLALAMLIGILIGIEREHRRPEKI
                     ELIAGVRSFTIVCIAGMISSFLAIKINPGILLISLAFFAGISILFIYIKNVILKTPGI
                     TGPIALYCTFLLGVLISYELFIPAVAGAVIITLLLAEKRPLHSFATKLTDEEILSAVR
                     FLAVIFILYPLTPDALYLDVINPRTILLIVIFVATISFASFIIMKNMGTEKGIPISGM
                     LGGLVNSEATTGALCAMAKKRHELIESSFKGIIMCNATMLIRNLAIALIVDRSGKIFL
                     LMLIPQIFIVIYSAAEVLGSKNNSHVSESIKLESPFALSPAIKFAIGFTGLSIVSKFA
                     NLWAGAAGVYATALGGFVSSAVVTASATALAVNGEVSYSTGAITAILASLVSTANKMI
                     LVKWSGPPELGGLINKAFTRFIIVGGIVLVIWGFFITYL"
     gene            complement(6376..7410)
                     /locus_tag="VKL21_00630"
     CDS             complement(6376..7410)
                     /locus_tag="VKL21_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HMB44304.1"
                     /translation="MKLLAIDVGMGTQDILLYDSQKNIENCFKMVLPSQTQIIARRIT
                     METGLKRDIVLTGETMGGGPCSVAVKRHLRADLCVYATEKAALTLDDSLEKTRKTGVV
                     IVKEDEVAELNASNIRMCDIDKTALSEAFALFDIEIPENLAIAVQDHGFSPDSSNRVF
                     RFDYFREIIEKRATLDSFVYKENIPERFTRMKAVQRTLPGAMLMDTGIAAIRGAILDE
                     KAHPPYLAVNIGNGHTLSGIIDNGALIALMEHHTQQMNREKLDDHLVRLCNGTIGFEE
                     VFKDGGHGCFVKEAIGFKNIGSILVTGPKREIIGGSKLDFHYATPYGDMMLTGCFGLA
                     DAYIHYDERS"
     gene            7840..7925
                     /locus_tag="VKL21_00635"
     tRNA            7840..7925
                     /locus_tag="VKL21_00635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7874..7876,aa:Ser,seq:gct)
BASE COUNT         2675 a         1502 c         1712 g         2244 t
ORIGIN      
        1 acccttatta cactttcaat actaaaggaa aagaagagac aatagattac cgtgtgccta
       61 tcgcaaggat cgaacaagaa agaaaagagg aggcaaggtt cttgccaggg ttggcgcgaa
      121 cggacgaacc ggttttcaat gtcccaaggc ttgggaaatc aaatcttcgc tcgtggcagg
      181 accatgaggt aattatgata ttggaggatg gaaggagagt ttaccgtttc tatccatggg
      241 aatccaaata cgctcttgtt gaaccctata attatactga tgtttcgatc tatgactatc
      301 ttaagagatt gaaggatgac aaggaaaata ttgacgatta ttcttcgatc tggcattatt
      361 tctgaatttt catgaataaa cgttacattg attagaaagg aggagaatct gcaataatga
      421 agaagtataa agtgatttgt ataacatccg gatctacagt tccacccgaa cccgatagga
      481 ttgaaagaga gatcacagcc atgacagagc aggggtggga tttcctgcaa attacatcag
      541 gcggcggcgg cacaggcagt ggaaatatta cttcctggat ttatttgatt ttcaaacgtg
      601 aatcttagtt atgaattaaa tgataaaaaa gaataagcgg ccatcattgg agaatattat
      661 agaaagtgag gatgtgtgtt tagagatact gcaccctggc ggattagata taacccggga
      721 actttcgcaa ctttgccata ttggcaaagc aataaaggtt cttgatgtgg gttcgggaaa
      781 aggtgaaagc gctttttacc tggtaagaaa tttcaactgc catataatcg gaatagatat
      841 ctcagatcat atgattaaaa gggcaaagag aaaagcaatt gatcagggcg taataataca
      901 gttcaagaaa ggtgatgccc atgacctgcc ttttaaagat aatacttttg atgcagtaat
      961 ttcggagtgc acaaccagta tcctggataa ggagagagct ttaggggaaa tggtaagagt
     1021 tgccagatcg ggggggtaca taggcattca tgacatttgt tggaaaaaag gtaccccaga
     1081 gcgccaaaaa caaaagctta cagaaattga attcaggagg ccggaaaccc tggaaggctg
     1141 gaaaacccaa tttgagaaag ccgggttgat agacgttatg attgaggaca gatcaaactt
     1201 gatccctgaa tgggaaaaat acatattgga aaagctgggc agaatcgcgc ggttcatgat
     1261 atttcttaag atcataaaaa attggggaat cgggggttta agaatgatta tggaatccag
     1321 gaggatcttc cagagccaga atacaggtta tgggatgatt gtggggaaga aaccatgagc
     1381 ccgggcacaa ggcaaaatag aatgcttaag aagaggtcaa ccaaaacagg tcttcctccc
     1441 ggggcactgg ttcatatcgg tgagaaaaaa gcagaaaaag tgaggatcaa tatccttgac
     1501 tatgatgaat atcaatttga ggaaaaagaa gctaagaaca ttgaggaatg ttttcccttt
     1561 aaagataagc caacgatcac ctggataaat attgatggtc tgcaccaggt tgatataatc
     1621 gaaaagatcg ggaaaaactt tgatctccat cctctccttc ttgaagacat tcttaacacc
     1681 gagcagcgcc ccaaaataga ggattttgag acctacatct acattgtttt gaaaatgctt
     1741 ctttatgatg aaaaaacaaa tgaaataaac tcagagcagg tcagtataat tttcgggcaa
     1801 aattttgtta tttcattcca ggaaaaagaa gtggatgttt tcaatccgct aagagagata
     1861 atcagaaccg gcaaaggccg tatcaggaaa atgaaggcag attatcttgc atatagttta
     1921 atagatgcaa tagttgacgg ctattttctg atccttgaaa aactcggaga gaatatcgaa
     1981 gatgttgaag aaatcatgat ggccaatccc tcacctgaaa cgttaaaggc tattcataaa
     2041 ctaaaaagaa ggatgatatc cctgcgtaag tccgtgtggc ctttaagaga ggtagtcagc
     2101 gcccttgaaa gatcagaatc atcgttgatc cacgaccaaa cacggattta tctaaaggat
     2161 gtttatgacc acacgatcca ggtgattgat acagttgaga cacacaggga tatgctttcc
     2221 ggtatgcttg atgtttatct ttcaagcatc agcaataagt tgaacgagat catgaaggtt
     2281 ttgacgatga tcgcaacgat atttattccc ctgactttca tagtaggtgt ttatggaatg
     2341 aattttgaat ttatgcctgg acttaaatgg tactggggtt atccggctat ctggggtgta
     2401 atgctaatga ttgtcatttc gatgctgtta ttttttagaa aaaagaaatg gatataaata
     2461 tatgaaactg tattttagat aatggagtgc taaaatgaga tttctttatg gcgattctac
     2521 tgaattcgat ctgcaaatag atttcctgag gcttttaaat aattttgtag atacaggagt
     2581 aagaactata aaatatgaaa atgctatttt cgatctcaag aatactatta ttgaaaaaaa
     2641 gagatataat gcttcggtaa ttgaagatat gaatggtttt gtcttaaatg tcgaaacagc
     2701 tatttctgga gcagttgcca gcagcaaaga gcaggagacc ttaattaaat atgcagaaaa
     2761 gagcaaagaa tactttaata aatttataga agaaagcaaa acaaaattct cagatgaaat
     2821 attaaaggaa atagcgcaac ttgaaaaaaa gatcaaagag gcctgtgaag agaacaggaa
     2881 gatcctggag tctttcttta tctatgatcc aataccaata gtagacgaaa aatatactat
     2941 cagggcaaca gaaaaaggct attctgcaca attgaagata gactgcgaga gtgggatttc
     3001 ctgccttttt catattgcat catcagaatc tcctttctcg agaaaccatg tcagagcccg
     3061 tgattttgta aaagatgtcc agatccctgc caggatgaaa aaacctttcc tgaaaaaaga
     3121 acttgtacct gatattgtaa atataaatga tttcattatt gttgaccttg ttctttcgga
     3181 taaagagcta gaggttgttt tcagaaaaag acttgatatt aaagctgagg ctttcagatt
     3241 aagaatgaaa tttgagaaag aattttcagt tgaagtgtca gaaaaagagg agaacggaac
     3301 ccagagaagc atcgatgata ttccagaatt gaaaaatgaa ttgaactatt taagacttca
     3361 ggagcttgga ttaaagataa tcgaaaaaac aaaatatcta tatcccaaga aacaaaagtt
     3421 agagaccata ctcctggaag aaaaagatgt atttgatgaa aactttattt ttgagttaat
     3481 gcagaaagtc gcagaaatct tcgcccctac aattgctgaa ataaaaaaac acagtccctc
     3541 gggcgaagaa ttgtctctta aatctgaaga tgaacacggg acaagacagg aaatatatct
     3601 taaaagatcg gaaattaagg ataagctcga tgagatagga gataagggca agactttgtc
     3661 agaatttctt aacataatta tttaaccttt gaagcaataa accgtttaat agtgaatcat
     3721 aaagaggcag gaatatggta cgattagaac gtgaagggaa ttcgttcgtg ttcattactg
     3781 gaaaaacgca gcctgcgcag gatataaata ttctgctaaa tgcattatca gaactacaaa
     3841 atagtgcgcc tgatacctct aaaataaaag aagggctttt gtttattgat aattcgaacg
     3901 agttcgatat tcgaaatgaa attaaaaatc ttctcagaaa agccctggaa agtgaaggaa
     3961 taagcttaca agatataagc taatatttca ttaatgccaa aattattcct gatccccacc
     4021 attaatgtct tagaagtaca tgatgcggta atgagggact ggctcagcgg tcttgaagac
     4081 actgccataa aagagcatct tgaatacccg ttcaagctgg agcgattttt tcctgaaagc
     4141 gcaaggcttg cactgatcaa tggaaataaa gttcacgacg agatccttgc tttatataat
     4201 attattgatc cgaaggcggt ttttcttaat atttcaaccg atctatatga gctggaaaat
     4261 aaatacaata aaaggctgat atcgccgcat gaattctggc gggaatatta tgagaacatt
     4321 tcaaaagata taagcgagcc ggcaagaaca ctttataaga tttacctgaa tgagataatc
     4381 gataaaaata ccaggctcat agaaagtaaa gaacgtcttc ctttgaatat acttttctat
     4441 gggatcaatc tgagatcaag agaccaactt attccggttt atgaaaaagc ttccgacatg
     4501 gtcgaagaat tcacattgca tatcgcacgg gcagttaatg aaataatgaa tcttcgcgag
     4561 aggccaaaac acctctggca tagttcatta aatgccaccc gggtggggat ctcatatgaa
     4621 gaaactgaaa aattttatga tgagctctta agcagattaa aaagactgct taaatataag
     4681 gtgagggaat atttcgtaaa aatcctgtgg gaacaagcca gggcatatgt tgagtcgtat
     4741 gaagaattcc ttgatcataa gataatagaa gatgaaaata aattttcaaa tattgctgat
     4801 ggactcaaaa tactggaatt acagtcagaa atatctggaa ttgtcttact ttgcgagccc
     4861 ggggattata ctgtgatcct tgagttctta aaaaataata tggaccacag gttttccatt
     4921 gcgcagataa gtcttatcag gctttttgaa aaaatgaggc cttttatcca gaagaacagg
     4981 ataatgcaaa acaattatga aatcgcgttg aatattctcg gacgcgagaa accaaaaaga
     5041 ttcacaaata agttatgaag aatccccata taaccagaac aattcctcca acaattatga
     5101 agcgtgtgaa tgccttgttg atcaatccgc ccagctctgg cggacccgac catttaacca
     5161 gtatcatctt atttgctgta cttaccaggc ttgccagaat cgcagttatc gcccctgtgc
     5221 tatatgaaac ctcaccattt actgcgagcg ctgttgctga tgccgttact acagcgctgc
     5281 ttacaaaacc acccaatgca gtagcataaa caccggccgc acctgcccag aggtttgcga
     5341 attttgacac aatggataat cctgtgaaac caattgcaaa ttttatcgct ggagaaagcg
     5401 cgaacgggga ttccaatttg atcgattcgc tcacatgcga attattttta gatccaagaa
     5461 cttcagccgc agaataaata acgataaata tttgaggaat tagcatcaat aaaaatattt
     5521 ttccgctccg atctacgatc agggcgatag caaggttcct gataagcatt gtagcattgc
     5581 acattattat tcccttgaaa ctggattcga tcaattcgtg tctctttttt gccattgcgc
     5641 aaagtgcccc tgttgtagct tcgctgttaa caagcccgcc cagcatcccg gagatgggga
     5701 ttcctttttc ggtccccatg tttttcatta ttatgaagct ggcaaagctt atggttgcaa
     5761 cgaatattac gatcaaaagg atggttcttg gattgataac gtccaggtat aatgcatcgg
     5821 gagtcagtgg atataatata aaaattactg caaggaagcg gacggcgctt aaaatctcct
     5881 catccgtaag ttttgtggca aatgaatgaa gcggtcgttt ttctgcaagc aaaagagtaa
     5941 taataacagc cccggcaaca gcagggatga ataattcata tgatatcaaa acgccaagaa
     6001 ggaaagtgca atatagtgcg ataggacctg tgatgcctgg tgttttcagg attacatttt
     6061 taatatatat aaataatatt gatatacccg caaagaatgc aagagagata agaagtattc
     6121 ccggattgat ttttattgca aggaatgagg agatcattcc tgcaatacag acaattgtaa
     6181 atgagcggac ccccgctatc aattctattt tctcaggccg cctgtgttcc ctttcaatac
     6241 caattagaat tccaataagc atagcaaggg ctaatttctc aaagaaatta aaattgacat
     6301 ccatattatt gaaaaactgc ggtaccagtt ccagcattga ttatcaacaa ttaatacttt
     6361 tataattata aatacttagc ttctttcatc ataatgaatg taagcatccg ccaatccaaa
     6421 acaacctgtc aacatcatat ccccataagg cgtagcataa tggaaatcca gttttgaacc
     6481 gcctatgatc tcccgctttg gacctgtgac caatattgag ccaatatttt taaaaccaat
     6541 cgcttccttc acaaagcacc catgcccgcc atccttaaaa acctcctcaa atccgatcgt
     6601 tccattacat aatcgcacca ggtgatcatc gagcttctcc cggttcattt gttgggtgtg
     6661 gtgttccatt aatgcgatga gcgctccatt atcaatgatc ccggaaagtg tgtgaccatt
     6721 tccgatattc accgcaagat acggcggatg ggctttctcg tccaggatcg ctccccttat
     6781 tgcagcaata ccggtatcca tgagcattgc gcccggcagt gttctctgca cagctttcat
     6841 tcttgtaaag cgctctggga tgttctcctt atagacaaaa gaatcaagag ttgcgcgttt
     6901 ttcaatgatt tccctgaaat aatcgaaccg aaagacacga ttgctactat cgggagaaaa
     6961 tccgtgatcc tgcacagcta tggcaagatt ttcagggatc tcaatgtcaa aaagagcaaa
     7021 agcttccgaa agggcggttt tatctatatc acacatcctg atattggagg cattcagttc
     7081 tgccacttca tcctctttta caatgaccac acccgttttc cttgtctttt caagactatc
     7141 atccagtgta agcgccgctt tttccgtagc atagacgcaa agatctgctc tcaagtgcct
     7201 ctttaccgct accgagcatg gtcctccgcc cattgtttct cctgtgagga caatatctct
     7261 ttttaatccc gtctccattg tgattctccg ggcgattatt tgggtctgtg atggcaggac
     7321 catcttaaag caattctcga tgttcttctg gctgtcatac aggagaatgt cctgtgttcc
     7381 cattcccaca tcaatagcaa gtagcttcat aggtacaaat cgagtgttca ttacgatttg
     7441 ataatatata gtcttccgct aattgcactt atgaaaaatc aaccacagat aattacagtg
     7501 tataactggc gaacttgtgt gcagaagaca aatccgatat ctgcataaaa gaccatttgg
     7561 ggaacgattt gatccagcga ttcaaatatg gatgagtgaa taatatgcaa ccggtgattt
     7621 catgttaaac cccgccagcg gcggcgcatt cgctggggtc tggggtcgca ccccagcgcc
     7681 gttaagataa tgttgaccga aagtgttaat agttacgctt gtatttgttc gttgttaact
     7741 attttgttgt aaaaagtgtt aaatagcaaa caggttatac agaaatacct ccttctaaat
     7801 gatggatatc gcttacgcta ttccatcaag tttcggagtg acgagatagc caagcccggt
     7861 atggcgcagg gttgctaacc ctgtgttccg caaggagctc gggggttcaa gtccccctct
     7921 cgtcgccttc tactaatgct ggtttttcgg gtaatcttca tcgcaagtaa aatcaaggag
     7981 caagaacctg atattttggc tttaaaagtc caattgcaga aaggaacgtt ttacactatt
     8041 ttggctgaag ttgagagcaa tctcagggtt gaaaaaaatg ggatagcgcg gatttgaacc
     8101 gcggtctaag cgtcccaaac gctcaaggat gga
//
LOCUS       DATGBN010000030         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_468236, whole genome shotgun sequence.
ACCESSION   DATGBN010000030 DATGBN010000000
VERSION     DATGBN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468236"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VKL21_00640"
     CDS             complement(<1..228)
                     /locus_tag="VKL21_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB44305.1"
                     /translation="MNTTTMLYVGIDVSKDKSDICIKGQLGNDLIQRFKIANNKADLG
                     KLYETIESIKSRTPGKSDVVFGMEATGIYSLP"
     gene            complement(505..1080)
                     /gene="hisB"
                     /locus_tag="VKL21_00645"
     CDS             complement(505..1080)
                     /gene="hisB"
                     /locus_tag="VKL21_00645"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HMB44306.1"
                     /translation="MREAKVSRKTNETEIEIKINLDGSGNTDINTGIPFFDHMLNSFS
                     KHGSFDLTIKARGDITIDDHHTVEDVGITLGSAIKKALGEKLGIERFADARIPMDEAL
                     ATAAVDISGRSFLVFDASFTNPKMGGFNSQNTRHFFESLTNNAGINVHMDVTGENDHH
                     KAEALFKAFGIALKRAVKITGTGVPSTKGML"
     gene            complement(1191..1478)
                     /locus_tag="VKL21_00650"
     CDS             complement(1191..1478)
                     /locus_tag="VKL21_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44307.1"
                     /translation="MKIREVIQEFVPCEICGNSDIKNFYFHVLVDKGSQPYVKCCECE
                     EEYYSERAKSILTRVKESRLVRTEPGTTFENKEGLINQESIHPLVKVLAKM"
     gene            1690..2385
                     /locus_tag="VKL21_00655"
     CDS             1690..2385
                     /locus_tag="VKL21_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase partner protein"
                     /protein_id="HMB44308.1"
                     /translation="MIRQRFVLDTSALTDTQTRELEGGNLDTAMGGILDIIAEARLHL
                     GISCYIPYPSVYTEMREFAKNNGCGEDTLAKIDTWLVKKTPDRYEVKIPSKIFYDYVD
                     FMRGRINKGMNVAEDAIWESATECLFRTTKAKTKSEIEPDIERNVIGGVINSFRDKYR
                     NALRYGILDSAPDIDVLLLSKELDAAVVASDMGIQKWAEQLGLRFVEAKSFPAMIKEY
                     LKRIKPDKVASMI"
     gene            2382..2690
                     /locus_tag="VKL21_00660"
     CDS             2382..2690
                     /locus_tag="VKL21_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44309.1"
                     /translation="MISTSVYIAAFGFVSIIFLTLRDIRIFRRTKLDSYRKGALRGMI
                     TGGLAWLGMIFTGNSGNEQIGLVIVFVAIYFNKKGKREDVFGDAPLMKRVLGETAFKK
                     "
     gene            2740..3561
                     /gene="hisF"
                     /locus_tag="VKL21_00665"
     CDS             2740..3561
                     /gene="hisF"
                     /locus_tag="VKL21_00665"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HMB44310.1"
                     /translation="MFTKRIIPCLDVTLDKAGGCVVKGIEFVNLKEAGDPVQLAKRYN
                     EQGADELVFLDITASHERRGTMIDVIERTADEVFIPLTVGGGINSTEAIRNILRAGAD
                     KVSINTAAVKTPAFIKQSADIFGSQCIVTAIDCRRNTDIGGHKNKTIIELENGTKAWY
                     EVVIYGGRTPTGLDTVQWAQKVEKLGSGEILLTSMDKDGTKDGYDLLITRTLSETLDI
                     PIIASGGAGNIEHMYDAFTKGKADAALAASIFHFGEYTIGQAKEYLKKKGVAVRI"
BASE COUNT         1083 a          742 c          813 g         1110 t
ORIGIN      
        1 cggcaaagaa tatatcccgg tggcttccat tccgaaaaca acatcgctct ttcctggtgt
       61 tctgcttttt atactttcaa ttgtttcata caacttaccc aaatctgctt tattgtttgc
      121 gattttaaat cgctggatca aatcgttccc caattggcct ttgatgcaga tatcggattt
      181 gtcttttgaa acatctattc cgacatataa cattgttgtg gtattcattt atctcgacct
      241 tctgattttg ctttattggt tagatcaaga gagtcaggtc cctcctccat cccttcttac
      301 acaagttcgc gagtaatatg tggtacattt atatatgtcc aaaacgtact tattagtgta
      361 ttaaaaggat gagagggcaa caggtttatc cacgggaacg aatcccaaac gcaatgtttg
      421 ttaccctctc gacctacttt tagataggtg caagaatgct ttattgttct cgcagaagtg
      481 ttgtatatac gaacgcagat tcaactatag catccctttt gtgcttggaa cacctgtgcc
      541 tgtaattttt actgctcttt ttaatgcaat tccgaatgct ttaaagagcg cttcagcctt
      601 atgatggtca ttctctcctg ttacatccat atgcacattg atcccggcat tgtttgttaa
      661 tgactcgaaa aaatggcgcg tgttctggga gttaaatccg cccatcttcg gatttgtaaa
      721 tgatgcatca aagacaagaa aactgcgccc gctgatatct acagctgccg ttgcaagggc
      781 ttcatccatg gggatacgcg catctgcgaa tcgctcaatg cccaactttt ctccaagcgc
      841 ttttttaatg gcgcttccaa gtgtaatacc cacatcttca acggtgtgat gatcatcaat
      901 tgtaatgtcg ccgcgcgctt tgatggtgag gtcaaaggag ccgtgttttg aaaaagaatt
      961 aagcatgtga tcaaaaaatg gaatacctgt gttgatatcg gtatttcctg aaccgtccag
     1021 attgatcttt atttctattt cggtctcgtt tgtttttctc gatactttcg cttccctcat
     1081 ggtgtgatta tattatttgg aattaatata aagctattgc aagtactttt tccagatcac
     1141 cctaattgat acaaacgaga agaactttca acttttgtat atccgaataa ttacatcttt
     1201 gcgagaactt tgacaagagg atgtattgac tcttgattta tcaggccttc tttattctcg
     1261 aatgttgttc caggttcagt tcttacgagt cttgattctt tcactctggt aagtatgctt
     1321 ttcgcacgtt ctgaatagta ttcttcctcg cactcacagc attttacgta tggctgggac
     1381 cccttatcca ccagaacatg aaaatagaaa ttttttatat cactattccc acatatttca
     1441 cacggaacga attcctgtat cacttcacga attttcatga taatcctcga tgtttgaagg
     1501 tggttcgtat atatataatt tatcactgtg ttaatataaa taataatgat gattattacg
     1561 tggatgagtc tgatcttttc ggaagtttac aacaagctaa agctttttat gtttacatga
     1621 gaatgatatc attaaaagag caatagatcc ggatataaat tttataatac taaaataata
     1681 ctaaaatata tgatacgaca gagattcgtt cttgatacaa gcgcactgac cgatacacag
     1741 acacgtgagc ttgagggtgg taatcttgac actgcaatgg gtggtatcct tgatattatt
     1801 gcagaggcgc gtctccatct tggcatcagc tgctatattc cttatccttc cgtatatacc
     1861 gagatgagag aatttgcaaa gaataacgga tgcggtgagg atacccttgc taagatcgat
     1921 acctggctcg taaaaaagac ccctgacagg tatgaggtga aaatcccatc caagatcttt
     1981 tacgattatg tggatttcat gcgcgggcgc atcaataaag gaatgaatgt cgcggaggac
     2041 gcgatatggg aatcggcaac cgaatgcctt tttcgaacta ccaaggcaaa aacaaaaagc
     2101 gagatagaac ctgatatcga gcgaaatgtg atcgggggag taataaattc cttccgggat
     2161 aaataccgga atgcattgcg ttatggtatc cttgacagcg cccctgatat tgatgttctg
     2221 ctgctttcca aagagcttga tgcggctgtg gtggcaagcg atatgggaat tcaaaaatgg
     2281 gctgaacagc tcgggcttcg tttcgttgag gcaaaatcct tcccggcgat gataaaagaa
     2341 tatctaaaaa gaattaaacc cgataaagtg gcaagtatga tatgatctct acttctgtat
     2401 atatagcagc attcggattt gtctcgataa ttttccttac cctgcgggat atcaggattt
     2461 ttcgccgcac aaaacttgat tcgtacagga aaggagcgct tcgtggaatg attacggggg
     2521 gcctggcatg gctggggatg attttcacag ggaattcagg taatgaacaa atagggctcg
     2581 tgattgtgtt tgtggcgatc tattttaaca aaaaaggaaa acgtgaagat gtattcgggg
     2641 atgcgccttt gatgaagaga gtgcttgggg aaacggcatt taagaaatag atataaataa
     2701 cacaaaacta tttcattctt aaactactaa aaaaggtcaa tgttcacaaa acgaataatc
     2761 ccgtgccttg acgtgactct tgataaagca ggaggctgcg ttgtaaaagg cattgaattt
     2821 gttaatttga aggaggcagg cgaccctgtg cagcttgcga aaagatacaa cgaacagggt
     2881 gccgatgagc ttgttttcct ggatattacg gcttcacatg aacgcagggg aaccatgata
     2941 gatgtgatcg aaagaaccgc cgatgaagtt tttatccctc tcactgtagg cggcggcata
     3001 aacagcacag aagccatacg gaacatcctg cgggcggggg cggataaagt atccatcaat
     3061 acggctgctg taaaaacgcc tgctttcata aaacaatccg ccgatatctt cggttctcaa
     3121 tgtatagtta cagctatcga ttgcaggcga aataccgata ttgggggaca caaaaataag
     3181 accataatag agcttgaaaa cggcacaaaa gcatggtatg aggtggtgat ttacggtgga
     3241 aggactccaa ccggtcttga tactgtgcaa tgggcccaaa aagtcgaaaa actcggttca
     3301 ggggagatac tgcttaccag catggataag gacggtacaa aggacggata tgacctactg
     3361 atcacaagga cgctttcaga aacacttgat atcccgataa tagcatcagg aggcgccgga
     3421 aatatcgaac atatgtacga tgcattcaca aaaggcaaag ctgacgcggc gcttgcagcg
     3481 agtattttcc atttcggtga gtacacgata gggcaggcca aggaatattt gaagaaaaaa
     3541 ggcgtagctg ttagaatttg atgtcgccaa cacatatggc tctcgccata cgtggagatc
     3601 tgtgttctaa ttcattccat ctgcttttat cggcgctcat ctgcggttaa tatttacgga
     3661 tatttaagaa tccgttataa ttgtaatgat tctctgtgtc ctctgagtgc tcagtggttc
     3721 aaccctacaa gtagataata aaagtctt
//
LOCUS       DATGBN010000031         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_41057, whole genome shotgun sequence.
ACCESSION   DATGBN010000031 DATGBN010000000
VERSION     DATGBN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41057"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..916
                     /locus_tag="VKL21_00670"
     CDS             83..916
                     /locus_tag="VKL21_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB44311.1"
                     /translation="MNLEILRDDLIRKCTHLGITLVGFAPIGRWKNPPGELPNVLSPW
                     IPEDFWPQSIYPEAKTVIVIGLPVSLPIVETAPSIYYHELYKTVNSMLDSRAYEIANF
                     LNEKGFSSIYLPRDGYGDIKILIEKPFAFFSHKHAAYLAGLGSFGHNNVLLTPQYGPR
                     VRFTSIFTSAEIRPDPIKIKDLCIHCLLCAKNCPVGAIPEKGIFPPMVDKRSCAVRSA
                     ELREEFRSPCGICIKVCPVGQDRELFGRKNMAIYSKDKKFEKYHNAWEHVRKYGSIEK
                     K"
     gene            1123..1770
                     /locus_tag="VKL21_00675"
     CDS             1123..1770
                     /locus_tag="VKL21_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HMB44312.1"
                     /translation="MIIVSEDEDEGDSNPFLFLGQPVHDSVLNINHDYRYLRMGYYVS
                     AHAETFGVEVTPNCTEIMDAYRNPLLIEKAKRQGLRTNYYRLITSLRSEIDAPALLFA
                     VNPFTNNSMKVVDSGSKLPGMIKRMSMGARFPVSLHPLTGEVVEIIQIFGESTNEETS
                     EFTKKFYEVFNIPICKLIVQIDDGGVVLNHCEPALKKEVKWDIVHDKVREIKQGI"
BASE COUNT          571 a          356 c          392 g          507 t
ORIGIN      
        1 tagcagaaag gttaagaaaa taacatttaa ggtttgaaga acgtcctttt agtacgcaga
       61 gttgaaaaaa atggattatc aaatgaatct tgaaatcctc agggatgatt tgatacgaaa
      121 atgcacacat ttggggatca ctctggtggg ttttgccccg atcggcagat ggaaaaatcc
      181 gcccggggag cttcctaatg tcttatcgcc atggatccct gaagatttct ggccgcaatc
      241 catttatccc gaagcaaaaa ccgtaatagt gatagggttg cctgtatcat tacccattgt
      301 tgagactgcg ccctccatct attaccacga actttataaa accgtgaatt ccatgcttga
      361 ttcaagggct tatgaaattg cgaatttcct gaatgaaaaa ggtttcagtt caatatacct
      421 tcccagggat ggctatggtg atataaaaat attgatcgaa aaacccttcg cttttttctc
      481 tcacaagcat gctgcatacc ttgccgggtt gggctctttt ggccacaata atgtcctgct
      541 tacaccgcaa tatggaccga gggtaaggtt tacatctata ttcacctcgg cggaaataag
      601 acccgatccc ataaaaatca aggatttatg cattcactgc cttctatgtg cgaaaaattg
      661 ccctgttggg gccattccgg aaaaagggat ttttcctccc atggtcgata aaagatcatg
      721 tgctgtgagg agcgcagaac tcagggagga attcaggtct ccgtgcggga tttgtatcaa
      781 agtctgcccc gtggggcagg acagggaatt gttcgggagg aagaacatgg ccatatattc
      841 aaaagataaa aaattcgaaa aatatcacaa tgcctgggaa catgtaagaa aatacgggag
      901 tatagaaaaa aaataagata agggttgatt ctcctttcag accgatgcgc gggattttat
      961 atttgcccca tcctcttgtg cacgattttc catgaccctg tgataagaaa actctcctcc
     1021 atattttcga tcatcggcct gatatgttcg tattccctcg tggtcagccg ttacttttat
     1081 atactcagct tataattata taatatcgga ctgatatcaa aaatgattat agtcagtgaa
     1141 gacgaagatg aaggggactc taatccgttt ctttttttag gacaaccagt acacgatagc
     1201 gtacttaata tcaaccatga ctaccgttat ttgaggatgg gatattatgt atctgcgcat
     1261 gctgagacct ttggagtaga agtcacaccg aattgtacgg aaataatgga tgcatacagg
     1321 aatccgcttt tgatagaaaa agcgaaaaga cagggccttc gcaccaatta ttacaggctt
     1381 ataacatctt tacgaagcga aattgacgct ccagcattat tgttcgccgt aaatccgttt
     1441 acaaataact caatgaaagt tgtggattcc ggctcaaaac tacccggaat gataaagaga
     1501 atgagtatgg gagcaagatt tcctgtttcg ttacatcctc ttacaggtga agtagtggaa
     1561 ataatacaga ttttcgggga atcaaccaac gaagaaacat cagaattcac aaaaaaattc
     1621 tatgaggtat tcaatatccc catctgtaaa ttaatagttc agatagacga tggcggcgtt
     1681 gtgctgaacc attgtgaacc tgcattgaaa aaggaagtaa agtgggatat cgttcatgat
     1741 aaagtgcgcg agataaaaca gggcatttaa tcctatgcaa atagcctgct ttgtggaagc
     1801 atacaatttt attattaaat ccgagc
//
LOCUS       DATGBN010000032         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_235347, whole genome shotgun sequence.
ACCESSION   DATGBN010000032 DATGBN010000000
VERSION     DATGBN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235347"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1438
                     /gene="mfnA"
                     /locus_tag="VKL21_00680"
     CDS             308..1438
                     /gene="mfnA"
                     /locus_tag="VKL21_00680"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HMB44313.1"
                     /translation="MNQKGCPEKEVLSLLKNAMQKDTSYDKVLSAMCTKPHPIAVKAH
                     MQFIASNMGDFGLFQGTKELEEKVIKMMGGMLGDGNACGYITTGGTESNIQALRTARN
                     LGKKERPNMIVPDSAHFSFDKIADLLGIEIRKASLDAEFKVDEASIADKIDKNTIALV
                     GIAGSTEFGQIDPIDRLAKLAISNNLFLHIDAAFGAFVIPFLDKKYDFDFSVKGVTSI
                     TADPHKMGMSTIPAGGLLFREEECLLPLEIDTPYLTIRKQHSLAGTRSGASVAGAYAV
                     MKHLGRDGYKKIVKRCMKMTDRLVEGTRELGVEPLIEPVMNIVALDVPGLDDVRKKLR
                     AKGWVTSITRNPKAMRLILMPHLGEEIIELFISDLGDCIRSN"
     gene            1539..1904
                     /locus_tag="VKL21_00685"
     CDS             1539..1904
                     /locus_tag="VKL21_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44314.1"
                     /translation="MNEYILYKYLELFIFALYLIFVFLLVQIWFLWKDVGKKELIYKS
                     IVNESFFKKNCIYIFLFSTFFMAHEFLEGLNIPGTMVFFEFLDVMAILALVLFAYNWH
                     IKLRSCVPKKSISKEFAFE"
     gene            1954..2589
                     /locus_tag="VKL21_00690"
     CDS             1954..2589
                     /locus_tag="VKL21_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44315.1"
                     /translation="MKCPTCNGECILPARKVLDDILGMYMACGSCPPDPMFIKNVPLS
                     DKIDENSGLCPKCGKRHLDFVMGNVLSILKEKGLFPQDALLREVGTPLISFGYQIPYP
                     PRLSGKNIVLIMDSITKDVAEEIVEKVPEIKGVIKRRGLQSQSIGILDTDSSPHIYEL
                     LSGCDMRCDVVLSLFGELCIYKNQSRIHIEFNNTKIKKWKIFTLRGNWKML"
     gene            2547..2966
                     /locus_tag="VKL21_00695"
     CDS             2547..2966
                     /locus_tag="VKL21_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44316.1"
                     /translation="MEDLYLKGELEDAVVVDGFCGPGTLGLLSVLAGARKVILNDAWL
                     PAIRNTILNIRVNSSLLGLNIVFENPDHNELIGDEPLLLAKATGKSEVMVYHGDIRKL
                     DKAVKGSDICLIDTFPSMNPARFMVACKDMAKKTVII"
     gene            complement(2963..4009)
                     /locus_tag="VKL21_00700"
     CDS             complement(2963..4009)
                     /locus_tag="VKL21_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44317.1"
                     /translation="MVVRKNISLEPDHIKKLESLILKHNGNLSAAIRDAIDITDVSLH
                     RYGSFEEAISKIAADKKNLTGREESIESGRNVLLSKPIFLWMLTSTKGIPLENEIFED
                     VLDPLKITTISELDKKINIISRESGWNCQVSLFCMDDNNPATATVTIAGDNEFYRDFV
                     AHLVIMFLAINKGLDIEVVHKRATSTRIDLKCREKGTQPVATIKYFGYLKDAMREFKP
                     REEFWKNLMEIYSSVNYNMVSIYKGNYEELLTSNSLMDAGIFESISKKHIASIPHQDF
                     LKLLKKTEESLLIIDKIEILENCVNIYHNYKNEKVIQKIRDYYISLLKANGHEYEAKY
                     STSLIVLNHICCNS"
     gene            complement(4081..4749)
                     /locus_tag="VKL21_00705"
     CDS             complement(4081..4749)
                     /locus_tag="VKL21_00705"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HMB44318.1"
                     /translation="MKLAALISGGKDSSFAIYKALQEGHKVTDLITIIPANEDSYMFH
                     SANIRLTELISEACGIPLISQLSTGEKEKELLDLKKAISSVKVDGVAVGAIESEYQAS
                     RVRGICDELGIKMYAPLWHMGSEGLLREMITLMDIRMVKVAAAGMDESWLGRIFDEKM
                     IEDLKALNRKYRVHIAGEGGEYESLVLDAPFYKKRIHLLETRNLWMGDHGIMKVVRAE
                     LGEK"
     gene            complement(4755..>4986)
                     /locus_tag="VKL21_00710"
     CDS             complement(4755..>4986)
                     /locus_tag="VKL21_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HMB44319.1"
                     /translation="ACGEAFDSVFMAAGTALVGILLEMKVYDAENEEHGARIAKSTIG
                     KALISTPLDVVDVEEFEGGKDTREKKKSSGKY"
BASE COUNT         1450 a          995 c         1017 g         1524 t
ORIGIN      
        1 cagaaacgcc aaaccaggca ttcattcgga agatgcatcc tgaggtctgg ctcggttttg
       61 cttttaagat gtttggatgg tgaattttat gaaaactgta caaaaggtaa aagaagttaa
      121 gactgaggaa attattctag gaaaagatat cgtgtgaact tcagcagatg tcattagcag
      181 tgcataaatc aaggggttta tatctatcaa gatgagttaa gtaaattcta tgattcttaa
      241 tggtattcgg tcttcggtgt tcggtaaaag actacccttt attaacatgt attccggagt
      301 ttaggatatg aaccaaaaag ggtgcccgga gaaagaagta ttgtccctgc ttaagaacgc
      361 tatgcaaaaa gatacgtcat acgataaggt actgagcgcc atgtgcacaa aaccccatcc
      421 tatagctgta aaagcccata tgcaattcat cgcgtcaaat atgggggatt ttggcctgtt
      481 ccagggaaca aaggaacttg aagaaaaagt aataaagatg atgggcggta tgctcggtga
      541 tggtaatgcc tgcggttaca taacgacagg ggggacagaa tccaatatcc aggcattaag
      601 gactgccagg aaccttggta aaaaggagcg gccaaacatg attgtccctg attctgcaca
      661 tttctcattc gataagatag cagacctcct tggaatcgaa atacgaaaag cttcgcttga
      721 tgccgagttt aaagtggacg aagcctctat tgcagacaag atcgataaaa acacaatcgc
      781 gcttgtaggt attgcaggtt caacggaatt cggacagatc gacccaatag ataggttggc
      841 caaacttgcc atatcaaata acctgttctt gcatatagat gcagcctttg gagcatttgt
      901 aatacccttc cttgataaga aatatgattt tgatttttca gtgaaaggag ttacctcaat
      961 aactgcagat ccacacaaaa tgggcatgtc cactatcccg gccggaggcc tgcttttcag
     1021 ggaagaagaa tgtctgcttc cacttgagat cgatacacca tacctgacta tcaggaaaca
     1081 gcattctctt gcgggcacac gaagcggcgc ctcggtggcg ggcgcttatg ccgtaatgaa
     1141 acatcttgga agggacgggt ataagaaaat cgttaaaagg tgtatgaaaa tgacagacag
     1201 gcttgtggag ggaacccggg aactgggagt tgaacccttg atcgagcctg taatgaatat
     1261 cgtagcgctg gatgttcctg gacttgacga cgtcaggaag aaattgcggg caaaaggctg
     1321 ggttacttca ataacacgaa acccaaaggc catgagattg attctgatgc cgcatctggg
     1381 cgaggaaatc attgagctgt tcatttccga tcttggagat tgcattcgaa gcaattgaat
     1441 tcgaattatc atagatgctt ttttggtcat caaatgaaaa ccacagaaac gcttatatta
     1501 tttcatattt atcttaagcc tgataggaag tcagatgtat gaacgaatat atattatata
     1561 aatatttaga gttgtttata tttgcattat atttaatttt tgtattttta ttggttcaga
     1621 tatggttctt atggaaggat gttggaaaaa aggagctgat ttataaatca attgtcaatg
     1681 aatctttttt taagaaaaat tgtatatata tatttttatt cagtacattt ttcatggcgc
     1741 atgaattcct tgaaggatta aacattcctg gcacaatggt attttttgaa ttccttgatg
     1801 taatggcaat acttgctctt gtattatttg cttataactg gcacatcaaa ttaagatcat
     1861 gcgttcctaa aaaatcaata tctaaagaat ttgcattcga ataattcctc aaattaacga
     1921 aagatattta gctttcccga aaaatataac cctatgaaat gccccacatg caacggggaa
     1981 tgcattcttc ccgcccggaa agtactggat gacatattgg ggatgtatat ggcgtgtgga
     2041 tcctgtcctc ctgaccccat gttcatcaaa aatgttcctt tatccgataa aatcgatgag
     2101 aattcaggtt tgtgcccaaa atgcggaaag aggcacctgg attttgtcat gggaaatgtt
     2161 ctttcgatat tgaaggaaaa agggttattt ccccaggacg ccctgcttcg tgaagttgga
     2221 acaccgctaa tttcttttgg ttatcaaata ccgtatccac caaggctcag tggcaagaac
     2281 attgttctta ttatggattc cataacaaaa gatgttgctg aagaaatagt cgaaaaagta
     2341 ccggaaataa aaggagtgat caagagaaga ggactgcaat cgcaaagcat cggtatcctt
     2401 gatactgaca gctcacccca tatatacgaa ttgctttccg gatgcgatat gcgatgtgat
     2461 gttgtcttat ccttgtttgg ggaactgtgt atttataaaa atcagtcaag aattcatata
     2521 gagttcaata acacaaaaat aaaaaaatgg aagatcttta ccttaagggg gaattggaag
     2581 atgctgtagt ggttgatggt ttttgtggtc cgggtacgct tggcctgtta agcgttcttg
     2641 cgggtgcgag gaaggtaatt ctcaacgatg catggcttcc tgcaatccgg aacacaattt
     2701 tgaatatcag ggtaaactca agcctcctcg gactcaatat tgtttttgaa aacccggatc
     2761 ataatgaact gatcggggac gaaccactgc tccttgcaaa ggccacgggg aagtcggaag
     2821 tcatggtcta tcatggggat atccgcaaac ttgataaggc cgttaaggga tcagatattt
     2881 gtttgataga tacattccct tctatgaatc ctgcccgctt catggttgca tgcaaggata
     2941 tggccaaaaa aaccgtaata atctaactat tgcagcagat atgattcaat actatcaggc
     3001 tcgtggaata ttttgcttcg tattcatgtc cgtttgcctt caggagcgat atataataat
     3061 cccggatctt ctgaatgact ttttcattct tataattgtg atagatatta acacaattct
     3121 ccagtatctc gattttgtct atgatcagca gggattcttc agtctttttc aagagtttca
     3181 aaaaatcctg atggggaata ctggctatat gttttttgga tattgattcg aatatacctg
     3241 catccattaa tgaatttgag gtaagtagtt cttcatagtt ccctttataa atggatacca
     3301 tattgtaatt cacagaactg tatatttcca ttagattttt ccagaattct tccctgggtt
     3361 tgaactccct cattgcatct tttaaatatc cgaagtattt tattgtggca acaggttgcg
     3421 ttcctttttc acggcacttc aggtctatcc tggtagaggt cgctctttta tgaacaactt
     3481 caatatcaag ccctttgtta atggcaagga acattattac aaggtgagct acaaaatcgc
     3541 gataaaattc attatcccct gctatggtaa ctgtcgcagt tgcaggatta ttatcatcca
     3601 tgcaaaagag agatacctgg cagttccaac cggattcacg gcttattata tttatctttt
     3661 tatcaagttc tgaaatcgta gtgattttca gaggatcaag aacgtcttca aatatttcat
     3721 tttcgagtgg aattcctttt gttgatgtaa gcatccacaa gaaaataggc ttgctcagaa
     3781 gaacatttct tcctgattct atcgattctt cccttccagt taaatttttc ttatcagctg
     3841 caatttttga gatcgcttct tcgaaggaac catatcgatg aagcgataca tcagttatat
     3901 caatagcatc ccggatagct gcgctgagat tgccgttgtg tttcaagatt agggattcaa
     3961 gttttttaat atgatctggc tcaagcgata tgttttttcg gacaaccatg atactactgg
     4021 aatatcactg agttaatcta ttataaacct tttctaaaag gtgtttccgc caaatttcaa
     4081 ttacttttct ccaagctctg cccgtactac tttcatgata ccatggtctc ccatccatag
     4141 atttcgggtc tcaagaagat gaattctttt cttatagaat ggagcgtcaa gcacaaggga
     4201 ttcatactct cctccttccc ccgcgatatg tactctgtat ttcctgttaa gcgccttcaa
     4261 atcctctatc atcttttcat caaaaatcct gccaagccat gactcgtcca tcccggcggc
     4321 cgcaactttc accatcctga tatccattag agtgatcatt tcccgaagaa gcccttcaga
     4381 tcccatatgc cacaatggcg catacatttt tattccaagt tcatcacaga taccccttac
     4441 gcgggaagcc tggtattccg attcgatagc gcctactgcg acaccatcaa ctttcacgct
     4501 gcttatggct ttttttagat ccaggagttc cttttccttc tcgccggttg atagctgcga
     4561 aatgagagga attccgcaag cctcagaaat aagttcggtg agccggatat ttgcagaatg
     4621 gaacatgtat gaatcttcat ttgcaggtat tattgttata aggtcggtta ccttatgacc
     4681 ttcctgcaat gctttatata ttgcaaatga cgaatcctta ccccctgaaa taagtgctgc
     4741 aagcttcatc cgaattaata ttttcctgag gactttttct tttccctggt atcttttcca
     4801 ccttcgaact cttcgacatc aacgacatcc agaggtgtgc ttatcaacgc tttaccgata
     4861 gtggattttg cgatccttgc cccatgctct tcattttccg catcgtacac tttcatttca
     4921 agcagtattc ccacaagagc agtacctgcg gccataaaaa cgctgtcaaa agcctcaccg
     4981 catgca
//
LOCUS       DATGBN010000033         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_507321, whole genome shotgun sequence.
ACCESSION   DATGBN010000033 DATGBN010000000
VERSION     DATGBN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507321"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1482)
                     /locus_tag="VKL21_00715"
     CDS             complement(55..1482)
                     /locus_tag="VKL21_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB44320.1"
                     /translation="MEKNTIILFNPMPVKHPVAILNKMTTSLQVPLVNVPLSLLALGR
                     MVRSEFDVRIINAVVDNDYKRNIVDISKNALCLAVSSMTCYQITDGIDVCKAVKELYP
                     HLPVIWGGYHPSTEPVQTLKNPYIDIVIRGQGELTFKELVHKLRNNKSLNDVKGISFK
                     DGDRIIENAAQEFKSLDLFPPIPYEMINVEQHVKGYKFAKRCLDYYASQGCAAGCDFC
                     SEPLFCGGRWSGLAAETVVSELEYLAKTYDIDTFMIRDSDFFINVPRVKKICTLLIDK
                     NPGMRLTSVNGRMEQLWRVGDDVWELFKKAGIQEIFFGFESGLQEALDIMNKGAKTSQ
                     IKTCTDKCIKHDIDIRGSFMVGIPGMDAKEEIIRTFEVINDIISAYGKMGKLSHMDIL
                     LSFFTPYPHTKLYIASLKNGLKPLDTLEDWGDFDQFDFKAPWYSQEYFDLVKEFRAGM
                     PMNSGCDFGEWCGFYEGIVKRLESI"
     gene            1733..>2017
                     /locus_tag="VKL21_00720"
     CDS             1733..>2017
                     /locus_tag="VKL21_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB44321.1"
                     /translation="MEPIIEFRSITKSYGDFKVLDNVTLGIEKGIIFGLLGPNGAGKS
                     TLIKILSCQSRPSSGHAYISGLDVVSDKKEVLSIIGVVPQENSFYDELTVN"
BASE COUNT          593 a          437 c          374 g          613 t
ORIGIN      
        1 tccgaaagga ttatcagatt catacgatta ctcctgtggt tttgaaatag caattcaaat
       61 actctctaat ctctttacta ttccctcata aaatccgcac cattccccaa aatcacaccc
      121 cgaattcatt ggcatccctg ccctgaattc cttcaccagg tcaaaatact cctgtgaata
      181 ccatggagcc ttgaaatcaa actggtcaaa gtcaccccaa tcttccagtg tatcgagggg
      241 cttcaatcca ttcttgagtg aggcaatgta caatttcgtg tgcggatatg gtgtaaaaaa
      301 cgagagcaga atatccatat gcgaaagttt tcccatcttt ccatatgcag agatgatgtc
      361 gtttatcact tcaaatgttc ttattatttc ttcttttgca tccattcccg gtattcccac
      421 catgaaagag ccgcgaatat cgatatcatg ttttatgcat ttatctgtgc aggtctttat
      481 ctggctagtc ttggcccctt tgttcattat atcaagcgcc tcttgcagtc cgctttcaaa
      541 accaaagaat atctcctgta tccctgcttt tttgaaaagt tcccatacat cgtcaccaac
      601 acgccagagt tgctccatcc taccattaac gcttgtgagc cgcatacccg gatttttatc
      661 aataaggaga gtgcatatct tcttcacgcg tgggacgttt atgaaaaagt cactgtccct
      721 gatcataaaa gtatctatgt catatgtttt tgctagatat tcaagctccg aaacgacagt
      781 ttcagctgca agtcctgacc aacgccctcc gcaaaataag ggctctgagc agaaatcaca
      841 tcctgccgcg cagccctggc ttgcataata atcaaggcag cgttttgcga acttatagcc
      901 cttgacatgc tgttcaacat taatcatctc atagggaata ggtgggaaaa gatcaagtga
      961 tttaaattcc tgtgctgcat tttctattat cctatcacca tctttgaatg aaattccctt
     1021 aacgtcatta agggatttat tatttctgag tttatgaact agttctttaa aagtaagttc
     1081 accctgcccc ctgataacaa tatcgatata tgggtttttg agggtttgca cgggctctgt
     1141 tgatgggtga taccctcccc agattaccgg aagatgtgga taaagttcct taacagcttt
     1201 acagacgtcg atcccgtcag tgatctgata gcaggtcatg ctgctgacag ccaggcacag
     1261 agcgttttta cttatatcaa caatatttct tttataatcg ttatccacca cagcattaat
     1321 tatcctgacg tcaaattcac tcctgaccat ccttcccagc gcaagaagcg acaggggaac
     1381 attgacaagc ggcacctgca gggatgtggt cattttgttc aggatggcaa caggatgttt
     1441 tacaggcatc ggattaaata aaatgatagt gttcttttcc atttatacac acttcgcgtt
     1501 ttgatatcat ataaatatta tgcaacatgg gcaaacagta tggacaagaa taaaacaatc
     1561 gaagttacga tttatccagt ggaaacaagg gggttatcat tagtattagt atcataattg
     1621 taatagtgtt atttggccat ttcctattca tgtacattta ataagttcta aatagaagat
     1681 ataataattg atagagtaat aaaaatatgt actaaaagaa ttcgaattaa acatggaacc
     1741 gataatagaa tttcgctcaa taaccaaaag ctatggggat ttcaaagtgc tggataatgt
     1801 caccctgggt attgaaaaag ggatcatatt cggtcttctg ggaccaaacg gcgccggaaa
     1861 gtcaacgctg ataaaaatcc tgtcatgcca atcaagacca tcttctggtc atgcatatat
     1921 atcaggcctt gatgtggtat cggataagaa agaagtcctt tccattattg gagttgtccc
     1981 ccaggaaaac agtttctacg atgagcttac tgttaat
//
LOCUS       DATGBN010000034         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_507378, whole genome shotgun sequence.
ACCESSION   DATGBN010000034 DATGBN010000000
VERSION     DATGBN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507378"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VKL21_00725"
     CDS             complement(<1..812)
                     /locus_tag="VKL21_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMB44322.1"
                     /translation="MHQIQKAVSYLKNDELVKEGTIMFLSGTIVGIFNYSYQVYMGRA
                     LGPEEYGVFGALFAIFYMMGIISQTLGTSTTQFVSKFVGEGKQIGFFIEGSLKRTILL
                     GSAVSMIFLIFRTGLMNLLKLPDVWPILILILILSLSWIIPIIDGSLRGMRRFSCLAL
                     SSISNASFKLIFGVGLVMAGFGVSGALFGVAIGAILGLFISFGFLKPYIKPNNPHDPD
                     FRYLSFYSYSLPVVFAMISLSIPSNLDVILAKYFFSPSEAGLYTSVSVMGKIV"
     gene            complement(839..1654)
                     /locus_tag="VKL21_00730"
     CDS             complement(839..1654)
                     /locus_tag="VKL21_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB44323.1"
                     /translation="MINKRWNSVSVVIPAYNENEMILNTIKECETSLTGMDHEIIIVD
                     DGSLDGTYEKVQEFARGNNNVRIINYGCNRGKGFALRYGFKHTKGNFVVFIDADMNIH
                     PRQILNFIKEMERTGADVVVGSKRHPESKIDYPLNRKIFSEIYYRFVKILFGIQVKDT
                     QVGLKLYKRKVLEDICPMVLVKKYAFDIEILANAYRLGYKIRDAPIEINMNFDSHVNK
                     KAIWNMFWDTCAIFYRMKVIHYYDNKLELNKNRITTIEHEKKKYMETTYSETC"
     gene            complement(1677..2669)
                     /locus_tag="VKL21_00735"
     CDS             complement(1677..2669)
                     /locus_tag="VKL21_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44324.1"
                     /translation="MENKVRSNAKTWLLVPLALMLLSLMLIPIVASAKVDVHIDQQND
                     SVRFEVKNAGDNPTYLLNSLTISNETGDSVYTSQEKSSADLLKLEPGVSYAFEWDIDD
                     VPEGKYIQKIYQGDDRRNLSAISFDFLRGKRQDKPILYTGKKFYKYGEDVDIAFMNMG
                     TYIIYVNVNNWEIENLETGEVVFRASQDCTFGYGNCADSFEPLRFLKTIEKTWDQKDS
                     NGNQVVPGKYDVTAEYSKKDPSSGSIRIETITTKKFYIRPVSPAPILRGDLNGNGVSA
                     DAGDLDLMEQASTGEIISDSRYDLNNNGIPADAGDLVLMKRASKGEIDLQKSTN"
     gene            complement(2659..2997)
                     /locus_tag="VKL21_00740"
     CDS             complement(2659..2997)
                     /locus_tag="VKL21_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44325.1"
                     /translation="MNKMNMALLILAILSITTLTGHALASDKDANISTNETIPNPVNT
                     TDVIEISETANVINITENSNETIVIESIIKDEQKKSSPGFGLTDLMITLITAVILIIQ
                     YKKGRIIYGK"
     gene            3320..>3555
                     /locus_tag="VKL21_00745"
     CDS             3320..>3555
                     /locus_tag="VKL21_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44326.1"
                     /translation="MEKNMKRIRKLAIALAALALLVSSISAQPMAIDFGVNDTSGDPN
                     TFVKVPVNITDVQNESIAGIIFDISFNSSVINLT"
BASE COUNT         1073 a          731 c          542 g         1209 t
ORIGIN      
        1 acaatttttc ccataactga tactgaggta tataatccgg cctcagaagg ggaaaagaaa
       61 tatttggcca atattacatc aagattagat gggatcgaaa ggctgatcat agcaaacacc
      121 acaggcaaag aataagaata aaacgacaaa tatctaaaat ccggatcatg gggattattc
      181 ggctttatat atggttttag aaagccaaaa gatatgaaaa gacccaggat cgcacctatg
      241 gccacaccga ataacgcgcc gctgacccca aatcctgcca tcacaagacc aactccaaaa
      301 atcagtttaa atgatgcatt ggatatactt gataatgcaa gacaagaaaa tcttctcatt
      361 cccctcagtg aaccgtcaat tattgggatt atccaggaga gagaaagtat caatatgagg
      421 atcagtatag gccagacatc cggtaatttc aataagttca tcagacccgt cctaaaaatc
      481 agaaagatca tggatactgc tgatccaagc aagattgtcc gtttcaaaga accttctata
      541 aagaaaccga tctgttttcc ttctccgaca aacttggata cgaactgtgt tgtacttgta
      601 cctaatgtct gtgaaataat ccccatcata taaaatattg caaaaagagc gccaaaaaca
      661 ccatactctt caggtccaag ggctcttccc atatagacct ggtaactata gttgaaaatg
      721 ccaacgatcg tgccggacaa gaacatgatt gtcccttctt tgacaagttc gtcgtttttg
      781 agatagctga ctgctttttg tatttgatgc attctagatt atttttgaaa taccagtatc
      841 agcacgtttc tgaatatgtg gtttccatat atttcttctt ttcatgttcg atggttgtta
      901 ttctgttctt gttcagctct aacttattat cataataatg gataaccttc atcctataaa
      961 aaatggcgca tgtatcccag aacatgttcc agatagcttt tttattgaca tggctatcaa
     1021 agttcatgtt gatctcaatc ggagcatctc tgatcttata ccccaaccta tatgcatttg
     1081 ccaatatctc aatatcaaaa gcatatttct ttaccaaaac cattggacag atatcttcca
     1141 gaacttttct tttgtataat ttcaatccaa cctgagtatc ttttacctga atcccaaata
     1201 atatctttac aaaccgataa taaatttcgc taaatatttt cctgttgaga ggataatcta
     1261 tttttgactc cggatgtctc ttcgacccca caacgacatc tgcgcctgtc ctctccattt
     1321 ctttgataaa attcaatatc tgtctagggt gtatattcat atctgcatct ataaaaacca
     1381 caaaatttcc ttttgtatgc ttaaatccat atcttaatgc aaatcctttt ccgcgattac
     1441 acccataatt tattattctt acattattat ttcctctggc aaattcctgc accttctcat
     1501 aagtcccatc aagactacca tcatctacaa ttattatctc atgatccatt ccggttaaag
     1561 atgtttcaca ttctttaata gtattaagaa tcatttcatt ttcattatac gcaggaatta
     1621 caacagatac actattccat cttttattta tcattaataa cacatccttt attcaattaa
     1681 tttgtggatt tctgcaaatc tatttcaccc tttgacgccc gtttcattag aacaagatca
     1741 ccggcatctg ctggtattcc attgttgttc aaatcatatc tggaatcaga aatgatttca
     1801 cctgttgacg cctgctccat aagatccaga tcaccagcat ctgctgatac gccattaccg
     1861 ttcagatctc ctctaaggat gggtgctgga gacacgggtc ttatataaaa ttttttagtt
     1921 gttattgttt cgatccgtat gcttcctgaa gaaggatctt tctttgaata ttctgccgtc
     1981 acatcatatt ttccaggcac gacctgattt ccattactat ctttttgatc ccaggttttt
     2041 tcaatggttt ttagaaacct gagcggttca aaagaatctg cacagttgcc atatccaaat
     2101 gtgcaatcct gggatgccct gaacactact tcccctgtct ctagattttc tatctcccaa
     2161 ttattaacat ttacgtaaat tatataggtg cccatattca tgaaggcaat atctacatcc
     2221 tccccatatt tgtagaactt tttacctgta tacaaaatcg gtttatcctg cctttttccc
     2281 cttaagaaat caaatgaaat tgcgctcaga tttctcctat catccccctg atagattttt
     2341 tgtatatatt tcccttcggg cacatcatca atatcccatt cgaatgcgta tgatacgcct
     2401 ggctccagtt tcaataaatc tgctgaagat ttctcttgag aagtatatac actatctcct
     2461 gtttcatttg atattgtcaa tgaattgagt aaatacgttg gattatctcc agcattttta
     2521 acttcgaatc tcacactatc gttttgctga tctatatgta catccacctt tgcagacgcc
     2581 acaattggga tcagcatcaa agacaatagc ataagtgcta acgggactaa taaccacgtt
     2641 tttgcatttg atctgacttt attttccata tattatccgc ccttttttat attgtatgat
     2701 cagaattaca gcagtaatta gtgttatcat tagatctgtt aatccaaatc cgggagacga
     2761 cttcttttgc tcatctttta ttatcgattc tattacaatt gtctcgttcg aattttccgt
     2821 tatattaata acatttgcag tttcactgat ttcaattaca tctgttgtgt tcacgggatt
     2881 gggaattgtc tcatttgtag agatatttgc gtccttgtcg gatgccagcg cgtgtccagt
     2941 caatgtggtg attgataata tcgctaagat caataacgcc atattcattt tgttcatctc
     3001 tacttctctt ttttaatttt atcctaagac ttaccttata cctgttctat atttaaggtt
     3061 tatcatatgt attgttatta tcatacaaga catgaaaagg atagtggcca actttattta
     3121 tattttttgg ccgataacat gcacacaaat aataatcata aataaaaaat accagcataa
     3181 ctatatactt tcaaaggcat catattccta atgggagaat tatggatgct aataatacct
     3241 taaagaacat tttcgctttt ctgagatatt aatgaagcat tcttcaattc gtgaaatcat
     3301 tgagtgagga gaatataaaa tggaaaaaaa tatgaaaaga attagaaaat tggcaatagc
     3361 tctggcagcg ttagcgctgc tagtatcgtc tataagtgca cagcccatgg ccattgattt
     3421 tggtgtgaat gacacttccg gagatccaaa cacatttgtt aaagtccccg tgaatattac
     3481 agatgtacag aatgaatcta ttgccggtat tatctttgat ataagtttta attcgagtgt
     3541 tattaatttg actaa
//
LOCUS       DATGBN010000035         8224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_468513, whole genome shotgun sequence.
ACCESSION   DATGBN010000035 DATGBN010000000
VERSION     DATGBN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468513"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VKL21_00750"
     CDS             complement(<1..699)
                     /locus_tag="VKL21_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HMB44327.1"
                     /translation="MNEEKPAQTNILIEEKKESCAIPQTPPDSKQAFCVALPVSGGRP
                     LKLIGDNPHDFISFLKDSSVAWLNFPVKDIKKEAEIIAMSLGFSSSLVPTLLSSYLSA
                     YEDRDTEMGLMLPAVNVKKFDVLISPILMLIRKDLIVTIHEENVTRLVRLSRYADAFM
                     RKIKPNLMLEDKLTIILTRLIDENINRNFDHLRQIEAQGDELSKILMDPLMPRGMIAP
                     EIHNMKHALISYMDT"
     gene            complement(758..2224)
                     /gene="argH"
                     /locus_tag="VKL21_00755"
     CDS             complement(758..2224)
                     /gene="argH"
                     /locus_tag="VKL21_00755"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HMB44328.1"
                     /translation="MNILRRGRLQVAPDQDIMRFTSSMEADKKIFNADIEVDKAHVVM
                     LKEQRIISDRDCSAILSGLEKIQKDGITALDTSYEDVHIALEARLIEMVGEDTGGRMH
                     SGRSRNDEVATCIRLTLRMELLCLLEEAIDLLGTLIKTAGKHQETIMPGYTHTQHAQP
                     TTLAHNLLAHANAFMRDIERIRSAYGHTNQNPLGVAAFASTGFAINRKRTTELLGFDS
                     AFENSMDAVSTRDFMIENISCFSNMMTNLSRMAEELILWSSSEFGFIELDDSFSSTSS
                     IMPQKKNPDIAELMRAKTGIVHGTLVSVLTICKALPYSYNRDLQEATPHLWRAVRTVR
                     ASVRMARGMIGTMSVKKDAMNRATDIGFMTATELADVIVQTTGIPFRTAHHIVGEIAK
                     TGTPPTISILDKISLEIIHEKLSDKGLTEKTIRKALDPVDNVRKRRVQGGPSPLETKR
                     QMAVIKKNIKVVKKDIRRFYEKINQASDKLSKICTKYT"
     gene            2327..3472
                     /locus_tag="VKL21_00760"
     CDS             2327..3472
                     /locus_tag="VKL21_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HMB44329.1"
                     /translation="MTEMDHNKQNYWKGKTISPMALEDKMTITQLVDEVFDGMGYNAK
                     RLAQACHLFKSMLDENSTVCLTLAGAMTPVGLGGGIIKMIEKGFVDWIVTTGANTYHD
                     LHFAYKLPVHQGDYNADDNDLAENDVVRIYDVYIDMQRTLIAQDKIIQTLTSKASEEN
                     MFPEKFSTAYYYKILGEEVLKTAKYPDRSFIASASKYKVPVFVPCFADSSIGMGTSYL
                     PILANGKSGSTELNPRNFVDPSPTMDVLESAAIVHDSMVHDIQRGVLEVGGGAPKNFL
                     QQTGPMISQIIGMECPGENYVIQVTVDRPDTGGLSGATINEGKSWGKIPKAGEGNVVP
                     YLDATVGIPIIFAYALENCKPRKTRKLSDKLPDITRELIKAARLNVE"
     gene            3548..3946
                     /locus_tag="VKL21_00765"
     CDS             3548..3946
                     /locus_tag="VKL21_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44330.1"
                     /translation="MKETHIIALMIGLTGLQAGLILVGILPALSVNSSANMLFLIARL
                     AIIGYTGWMFSGRGFKEAAIKGGIVTLVSVITVYLGVFIGIIVHKPVLGISFASQPNL
                     LFYLLIMGIINVMFGAILAVIIALIGRKFK"
     gene            4118..4282
                     /locus_tag="VKL21_00770"
     CDS             4118..4282
                     /locus_tag="VKL21_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44331.1"
                     /translation="MKYTVGDTVRFKLESGEVQEGNIQFIEKNRSEETFYINSFNKWA
                     YKVSGRKIIS"
     gene            complement(4708..5871)
                     /locus_tag="VKL21_00775"
     CDS             complement(4708..5871)
                     /locus_tag="VKL21_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44332.1"
                     /translation="MKQKKSAIANNKPLMIIIILAIALFAIATNYLPAGCAAGDCTIP
                     PTSKPKPTPKVTPAHFPSPAIKHTPIGNPDLGIFNREAVDSVDDPLLKWQIIASPSIL
                     LNYSKSKGVFFNFWSDWSKSKSKWTDIGPDGFIEKRFALANKYKKKFEYAPFLLQEEM
                     YGPIALSTTAEPLNTWKLEGNATFNGVEVKLLKTWIIENKGKAWDQNWWNGLKGGSSD
                     PLLKEWEKYYTTNLYRAWNEYIHSLGKKTAVIGVLSLKNKDYEGDLEYSMPANKYVFA
                     NYDAIIDYSYPNSTTQISYSLNKIKFIRSKFKGKIFWVLTSNFPTTTTGASWWSTELA
                     RSEFKAVAPYADVILTYPFVDMHTRTNPYPQYLISFYETWGQGVKKASRIQDE"
     gene            complement(5990..>8224)
                     /locus_tag="VKL21_00780"
     CDS             complement(5990..>8224)
                     /locus_tag="VKL21_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB44333.1"
                     /translation="PLAYIDYAFFPEQKNIRFFKDNNIPFFLGIQSLHPITDFDLVLI
                     SNSFTLELFNLPYLFSNTGIPRMKSNRQTLRPIVIMGGSNVLMAQCAISNRGDSFIDA
                     LFFGEGEGAVGRISTILNENREKPNREVLVILEKEVKGFFDIRLPIPDSISAASSPHA
                     SYILTDYPILNTDIENTVKLQITRGCPCFCSFCFEGHTRKPFREYKPEDIYSKALEVK
                     IKHAPVEFDFLSFNFNLHTGISKIIADLNEIAKFVNFKSQRIDIIAMRPELLDLEILS
                     GKRTYTFGVEGINDRIRRFLHKSLSEKELLISLEHIYSRKPRQIKLFFIITGLENEND
                     CREFKDFIMKLKNLKAKLSPGCRTIMSFGLLTNLPFTPMQFSQTIKDPERIKKIKGNI
                     KRDCETNNFEFRMAQDIEDFLICQHIVLAGFECFDVICGFAKKGGYFSGENIIGDKTS
                     LILSLKSASGDRIYDAKDMKYVFPFENVKGTPVKSFLFRMYNEAKNFTDNGYCLLGKG
                     ECIGCGGCEEQKRLSLPEVKREDLDRLKNTVEKKKRPGILQAEVSLKESGRHLTPEAK
                     CSFIGNAILEQLPALARDYLACRQVQNMEVSKGYGFLFGRFLYDFEFSGSQEIFLSYL
                     KEKIIDSSLLSILFLTGREIGNDFRIVSQWNDPLEYSFQNHLQKYLLSKGLGFNIKKH
                     EPFICFEVAAKDRKKKLLNWVEFQQDRNNVTFVLETGSNFLIIDMLKYLFVDEWMNAK
                     VESI"
BASE COUNT         2391 a         1646 c         1573 g         2614 t
ORIGIN      
        1 cgtatccata tagctgatca gcgcatgttt catgttgtgg atctcaggag cgatcattcc
       61 tcttggcatc agtggatcca tcaggatctt gctcaattca tccccctggg cttcaatttg
      121 tctcagatgg tcaaaattcc tgttgatatt ttcatcgata agtcttgtca ggattattgt
      181 caatttatct tccagcatca gatttggctt tattttcctc atgaaagcgt cagcatagcg
      241 ggaaagacgc acaagacggg tcacattttc ttcatggatt gtcactatca ggtcttttct
      301 tatgagcatc aagatggggc tgatgagaac atcaaatttc ttcacattga ctgcaggaag
      361 catcaatccc atttcggtat ccctatcttc ataggcagaa agataactgg ataagagcgt
      421 gggcacgagg gaagaactga atcccaggga cattgcaatt atctccgctt cctttttaat
      481 gtctttaact ggaaaattta accatgctac agaagaatct tttaagaatg aaatgaaatc
      541 atggggatta tcccctatta attttaaagg tcttcccccg gatacaggca gcgctacaca
      601 gaacgcctgt ttgctgtcag ggggggtctg tgggatagcg caagactctt ttttttcttc
      661 gatcaatata ttagtctgag caggtttttc ctcattcata atccctcttt agtccaaaac
      721 catttatgca atctatcata tttatgtctg ccttacttca cgtatatttt gtacagattt
      781 ttgaaagttt atctgatgcc tggtttatct tttcataaaa tcgtcggata tcttttttca
      841 cgaccttgat attttttttg ataacagcca tctgccgctt tgtttcaaga ggtgaaggtc
      901 ctccctgtac ccggcgtttc ctgacattat ccacagggtc aagagccttc cggatagttt
      961 tttcggtaag acccttatca cttaatttct catgtattat ttccaatgag attttatcaa
     1021 ggattgaaat agttggaggt gttcccgtct ttgcaatttc cccaacaata tggtgggcag
     1081 tccgaaaagg aataccagta gtctggacta tgacatcagc aagttcggtc gcagtcatga
     1141 aacctatatc tgttgctctg ttcatagcat cttttttaac actcatcgtt cctatcatac
     1201 cccgggccat ccgaaccgag gctcgaactg tcctgacggc gcgccacagg tggggtgttg
     1261 cttcctgaag gtcgcgatta tagctgtatg gaagggcttt gcatatcgta agaacagaca
     1321 cgagagttcc gtgaacaatc ccggtctttg cacgcatgag ttcggctatg tcgggattct
     1381 tcttttgtgg catgatagag gatgttgaag aaaaactgtc atcaagttcg ataaaaccga
     1441 attccgatga actccacagt atcagttctt ctgccattct actcagattt gtcatcatat
     1501 tggaaaaaca gcttatgttc tcgatcataa aatcgcgtgt actgactgca tccatggaat
     1561 tttcgaatgc cgaatcaaac ccgagaagtt cggttgtcct tttcctgttt atcgcaaagc
     1621 ctgtggaagc aaatgcagca actccgagtg gattctggtt agtgtgcccg tacgccgatc
     1681 taattctctc aatatcacgc ataaaggcat tcgcgtgagc aagaagattg tgagcgagag
     1741 tggtgggttg tgcatgctgg gtgtgagtgt acccgggcat tatagtttct tgatgttttc
     1801 cagcagtctt gataagcgtt cccaaaaggt ctatagcttc ctccaataga cataagagtt
     1861 ccatcctcag ggtaagacgt atgcatgttg ccacttcgtc attgcgtgac cgtcccgaat
     1921 gcatcctccc accagtatct tcacccacca tttcaatgag gcgcgcttca agagcaatat
     1981 gcacgtcctc ataagatgta tctagagccg ttatgccatc tttttgtatt ttttcaaggc
     2041 ctgataggat ggcggaacaa tctctatcag atattatacg ctgttctttg agcataacaa
     2101 catgcgcctt gtcaacctca atatcggcat tgaaaatctt tttatcggct tccattgatg
     2161 atgtgaagcg catgatatcc tggtccggtg ctacctgaag ccgccctcgt ctcaatatat
     2221 tcataaactc tctttaattc atgtcaacat ttataaatat aggggcttta attaaatatc
     2281 tatctgcata tttgattaaa agcaatatat cgggtatcga actggaatga ctgaaatgga
     2341 tcacaacaaa caaaattact ggaaaggaaa aaccatctca ccaatggctc ttgaggataa
     2401 aatgaccata acgcagcttg ttgatgaagt attcgacggt atgggatata atgcaaaacg
     2461 attggcgcaa gcgtgtcatc ttttcaaatc catgctggat gagaattcaa ctgtatgcct
     2521 gacactggca ggcgcaatga cccctgtggg tcttggaggg ggtattatta aaatgattga
     2581 gaaagggttt gttgattgga tcgtcacaac aggcgcaaat acttatcatg atctgcattt
     2641 tgcatataaa cttcctgtgc accagggaga ttataatgcg gacgataatg atcttgctga
     2701 aaatgatgtt gtgcgcatct atgatgttta tattgatatg caaagaactc ttattgccca
     2761 ggataagatc atacagacgc ttactagtaa agccagtgaa gaaaacatgt tccctgaaaa
     2821 attctcgacc gcttattatt ataaaattct tggtgaagaa gttcttaaga ctgcaaagta
     2881 tcccgataga tcttttatcg catccgcttc aaaatacaaa gttcccgttt tcgtcccctg
     2941 tttcgcggat tcatcaattg gcatgggaac aagttacttg cctatccttg caaatggaaa
     3001 atcaggctca actgaactaa atccccgaaa tttcgtagac ccgagcccca caatggatgt
     3061 ccttgaaagc gctgccatag tgcatgacag catggtacac gatatccagc gaggagtcct
     3121 tgaagtggga ggaggcgcgc caaagaattt ccttcagcag acaggcccca tgatctccca
     3181 gatcataggt atggaatgcc ctggagagaa ttatgtgatc caggtaacgg tcgaccgccc
     3241 ggatactggc ggactttcag gagccacaat aaacgaaggc aaatcatggg gcaagattcc
     3301 aaaagcagga gaaggaaatg tggttccata tcttgatgct acggtaggca ttcccataat
     3361 cttcgcatat gctcttgaaa actgcaagcc aagaaaaacc aggaagctga gcgacaaatt
     3421 gccggatata acgagggaat tgataaaagc tgcaaggctg aatgtggaat gattccctgt
     3481 tttgtattcc tggcagatta accttcaacg aatatatata gtattacaaa aaatattata
     3541 ggagattatg aaagaaacac acataattgc cctgatgatc ggattaacgg gattacaggc
     3601 gggattaata cttgtcggga ttttgcctgc attatccgtc aattcttcgg ccaatatgtt
     3661 gttcctaatt gcccgcttag caattattgg ttatacggga tggatgtttt caggtcgtgg
     3721 tttcaaagaa gctgctataa aaggcggcat tgtaacgctt gtatcagtta ttacagttta
     3781 cctcggtgtt tttatcggga taatagtgca taaaccggtt cttggaataa gttttgcatc
     3841 acaaccaaat ttgcttttct atttacttat tatgggcata ataaatgtca tgttcggagc
     3901 tatactcgca gtgattattg cgctaatcgg acggaaattt aaataatgat atgcatgaaa
     3961 ataatcacga ttcacacgaa ttacacactt gagattatat acaggtacat ttaccataat
     4021 attcaaatta ttgatcaaaa taggaggcac acaacatggg tttaccacga agaaccataa
     4081 ggaaaattat gattaactca aagacgaggg taaatatatg aaatacactg tcggagatac
     4141 cgtacgattc aaacttgaat ctggtgaagt acaggaagga aatattcaat ttatagaaaa
     4201 gaacaggtcc gaagaaacat tttatataaa tagtttcaac aaatgggctt acaaagtttc
     4261 tggaagaaag attatttcgt aatatcttca aatctaacct ttcctaattc ttcttgaagg
     4321 acagaaatgt atttcggaat ttcatccggt tgataaatat gaatatattt tattcgacac
     4381 gaaccttttc gaacatattt gcatttcaag aatccaagca taacaagctc tcttattttg
     4441 tcagtaacct ggcttgtaga taggccgcct atatttgatt catctgctaa cctttgatat
     4501 tccaaaacaa catcattaac tgttattctt actttctttt tatcccggtc atgtaacctg
     4561 agaattgcat ataatatgag tttgatgtga agtttgaaac atttgattaa aaaaataaga
     4621 cataaacacg ttcatccaac tctatttcct gaacgtttcc acattttcat cctgtaagca
     4681 tacagttccg tagttatttt tcaggtacta ttcatcctga atgcgtgagg ctttcttcac
     4741 cccttgaccc caagtttcgt aaaagcttat caaatattgt gggtatggat tagttctagt
     4801 atgcatatct acaaatggat atgtcaaaat tacatctgca tatggcgcaa cagccttaaa
     4861 ttccgatcgt gccaattccg tcgaccacca tgaagctccg gtagtagttg ttgggaaatt
     4921 agaagttaat acccagaata ttttaccttt gaattttgat ctaatgaatt ttattttatt
     4981 caaagagtat gatatctgtg tagtgctatt tggatatgaa taatctataa ttgcatcata
     5041 atttgcaaat acatatttat ttgcgggcat tgagtattct aaatcaccct cataatcttt
     5101 atttttcaat gataatacgc caatcaccgc agttttcttg ccaagactat gtatatattc
     5161 attccatgcc cggtaaagat ttgtggtata atatttttcc cattctttaa gcaatgggtc
     5221 agaggaaccg ccttttaatc cattccacca attttggtcc catgctttac ccttgttttc
     5281 aatgatccat gtctttaata atttaacttc tacaccattg aatgttgcat taccttccag
     5341 tttccatgtg ttcaatggtt ctgccgtggt agataaggct atcggcccat acatttcttc
     5401 ttgaagtaag aatggtgcgt attcaaattt ttttttatat ttattcgcta acgcaaatct
     5461 tttttcaata aatccatcag gaccgatatc tgtccactta gattttgatt tagaccaatc
     5521 tgaccaaaaa ttaaaaaata caccttttga ttttgaatag ttaagtaata ttgatggcga
     5581 agctattatt tgccatttca ataaagggtc atctactgaa tctacggctt ccctattgaa
     5641 tattccaaga tcagggttac ctataggagt atgtttaatt gctggagaag gaaaatgtgc
     5701 gggggtcact tttggagttg gtttgggttt ggaggttggc ggtatagtgc aatctccagc
     5761 tgcacacccg gcaggcaggt agtttgtcgc tattgcaaat agagcaatgg ccaatattat
     5821 gatgatcata agtggtttat tattggcaat tgctgatttt ttttgtttca taattcaatt
     5881 ccttttcagg ataaatatga tgttagaaga atttaaacga gatttaattg gaaagaatcg
     5941 attgctcatg ctcatgcaat tagaggccat ctattaaaac atttctgtgt caaattgatt
     6001 caaccttcgc attcatccac tcatcaacaa aaaggtactt gagcatatct attatcaaga
     6061 aatttgaacc cgtttcaaga acaaatgtca cattatttct gtcctgttga aattcaaccc
     6121 aattcaggag cttttttttc ctgtccttcg ctgccacctc aaaacatata aatggctcat
     6181 gtttcttaat attaaatcca agtcctttag atagcaggta cttctgcagg tgattctgga
     6241 aactgtattc taaaggatcg ttccattgtg atactatcct gaaatcattt ccgatttctc
     6301 ttcctgttaa gaataagata ctcaataatg atgaatcaat gatcttttct ttaagataag
     6361 aaagaaaaat ttcctggctg cctgaaaatt caaaatcgta caggaatcga ccgaaaagga
     6421 acccatatcc ttttgaaact tccatattct gcacctgtct gcatgcaaga taatctcttg
     6481 caagagcagg taattgttca aggatagcgt ttcctataaa tgagcatttt gcttcaggcg
     6541 tcaggtgacg tccggattct tttaaagaaa cttctgcttg cagtatccct ggccttttct
     6601 ttttctccac ggtattttta agcctgtcga gatcttcccg cttcacttcc ggaagcgata
     6661 agcgcttttg ttcttcgcat cccccacacc cgatgcattc acctttccct aaaaggcaat
     6721 aaccattgtc cgtgaaattt tttgcttcat tatacatcct gaaaagaaat gatttaacag
     6781 gtgtgccttt tacgttttca aaaggaaaaa cgtatttcat atctttggca tcgtatattc
     6841 tatcaccgga tgctgacttt aaggacagga taagcgaagt cttatccccg ataatatttt
     6901 caccgctaaa gtatcccccc tttttagcaa atccgcaaat cacatcaaag cattcgaatc
     6961 ctgcaagaac tatatgctgg cagatcagga aatcttcaat atcctgggcc atcctgaatt
     7021 caaaattatt tgtctcacaa tcccttttga tatttccttt gatctttttt attctttcag
     7081 ggtcttttat tgtttgactg aattgcatag gtgtaaaagg aagattggtc agaagaccaa
     7141 aactcataat cgtcctgcag cctggcgata attttgcttt taaatttttt agcttcatta
     7201 tgaaatcttt aaattccctg cagtcatttt cattttcaag acctgtaata ataaaaaaca
     7261 attttatttg tcttggtttt cttgaataaa tgtgttcaag cgatatgaga agttcctttt
     7321 ctgacaggct cttatgtaaa aaccgtctta tcctgtcatt tattccttcc actccaaagg
     7381 tataggtcct tttcccggaa agtatctcaa gatcaagaag ctccgggcgc attgcaataa
     7441 tatctattcg ctgactcttg aaattcacaa attttgctat ttcattaagg tcagcgatta
     7501 tctttgagat accggtatga agattaaaat taaagctaag gaaatcaaac tctacaggcg
     7561 catgttttat tttcacctca agcgcttttg aatagatatc ttccggctta tattccctga
     7621 agggctttct tgtgtgtcct tcaaaacaga atgagcaaaa acacgggcat ccccgggtta
     7681 tctggagctt tactgtgttc tcgatatctg tgttcaatat cggataatca gtaaggatat
     7741 acgaagcatg tggcgaggaa gcagcgctaa tgctatccgg gattggcagc ctgatatcaa
     7801 agaacccttt tacttccttt tcaagaataa ccagaacttc tctatttggt ttttccctgt
     7861 tttcatttaa aatagttgag attctcccca cagcgccttc accctccccg aaaaataacg
     7921 catcaataaa agaatcaccc ctgttagata ttgcacattg cgccataagg acatttgagc
     7981 cccccataat tacaatgggg cgaagcgtct gtcgattgga tttcatccgg ggaatcccgg
     8041 tatttgaaaa gagatatggc aaattgaata attccagagt gaaagagttg gatatgagaa
     8101 caaggtcaaa atctgtaatg ggatgaaggg attgaatacc aaggaagaat ggaatattgt
     8161 tatccttgaa aaaccggata ttcttttgtt caggaaaaaa tgcataatca atatatgcca
     8221 gagg
//
LOCUS       DATGBN010000036         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_2532, whole genome shotgun sequence.
ACCESSION   DATGBN010000036 DATGBN010000000
VERSION     DATGBN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1717
                     /locus_tag="VKL21_00785"
     CDS             23..>1717
                     /locus_tag="VKL21_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HMB44334.1"
                     /translation="MKFEIIFLLAFLIFSLVRAYSPDFYWTAGEKFMDMTFINSMLRT
                     TGFPPLDPWMSGTVIYYYYFGYLIVANLINITGILPSIAFNLATASFFALSFTTALGV
                     GFNLTEKIKYGIITAFFVTIAGNLVGFFQLMDIFRKENVINNMFSFNYWTSSRVIPDT
                     INEFPFFSFLHGDLHAHMISITFQLLVLLLLLNIMKSNSFGLDSILIIGLSIGFLYPL
                     NTWDYPVYLILAILVVAYQIRKKDYLNSFASIAAMAAASYLLYLPYHLSYKIDKTILF
                     VPSGRTSLIFYLAVYGLFLFLIYTFVLNRNLNKKLFKLSFISLIIAALITIYEVKLFI
                     SNDVTNPKTNEFELLILLIPLLVLSGISIFKEKDKNNVFILILIIGGVFTSLFCEFFY
                     IQDALGNGNSIFIRLNTVFKMYLQNWILWGVCAGYIVFLFRDHFAPKKAWGVAAVLLI
                     LFVSIYPVFATIGKSVGFAGIPELDGGAYVKKEHPQDYEAILWFMNLTGHPVVLQAPV
                     ELYQWNSAITTFSGLPTVIGWGGHELNWRFPKRSEIDMRWSDVGRMYTSGDIREVEK"
BASE COUNT          475 a          307 c          330 g          605 t
ORIGIN      
        1 tttttttgat aaaaaataca ttatcaaatt tgaaataata tttttactcg catttttgat
       61 tttttcattg gtacgagctt atagtcctga cttttactgg accgcaggtg agaaattcat
      121 ggacatgaca ttcattaata gcatgcttag aaccaccgga tttcctcctc ttgacccatg
      181 gatgtcaggt acggtgatat attactatta tttcggatat ctaatagtag cgaatttgat
      241 aaatattacg ggaattttac catcgatagc tttcaatctt gccacagcct cattttttgc
      301 tttatcattc acaactgctt taggtgtggg atttaatctc acagagaaaa taaaatatgg
      361 gatcatcaca gcattttttg taactatcgc tgggaatctc gtaggttttt tccagttgat
      421 ggatattttc agaaaagaaa atgttatcaa taatatgttc tcgtttaatt actggacaag
      481 ttcgcgggtc attcccgata ccattaatga attccctttt ttcagcttcc ttcatggcga
      541 tttgcatgca catatgatat caattacttt ccagcttctg gttcttcttc ttcttttgaa
      601 tatcatgaaa tccaactcat ttggcctgga ttcaatcctg attattggac tttcgatagg
      661 ttttctatat cccctgaata catgggatta tccggtatat ctgatcctgg cgattctggt
      721 ggtcgcttat caaataagaa aaaaggatta cctgaattct tttgcatcga tagcagcaat
      781 ggccgctgca agttatttgt tatatctgcc atatcatctt tcatataaga tagataagac
      841 gattttattt gttccttcgg gaagaacatc tttgatattt tatctggcag tttacggatt
      901 attcctgttc ctgatataca cttttgtcct gaacaggaat cttaataaaa aattattcaa
      961 gttatcattt atctcgttga taatcgctgc attaataacg atatatgagg ttaaactttt
     1021 tatttcaaat gatgtgacaa acccaaaaac caatgaattc gaacttttga tcttgctgat
     1081 acctcttctt gttctgtcgg gaatttctat tttcaaagag aaggataaga acaatgtttt
     1141 tattttgata ttgatcattg gcggtgtatt tacatcactt ttttgtgaat ttttttatat
     1201 acaggatgca ctgggaaacg ggaattctat tttcatccgt ttgaacacgg tttttaaaat
     1261 gtatctccag aactggatct tgtggggtgt ttgcgccggt tatattgttt tcctgttcag
     1321 ggatcatttt gctccaaaaa aagcatgggg agttgcagca gtcctcctga tactgtttgt
     1381 ttcaatatat cctgtctttg ccacaatagg aaaatccgta ggttttgcag gtattcctga
     1441 acttgatggc ggagcatatg taaaaaaaga gcacccccag gactacgagg ctatattatg
     1501 gttcatgaat ctcacaggac atccagttgt ccttcaggct cccgtggaat tataccagtg
     1561 gaatagcgcc attacaacct tttcagggct tccaacagtt atagggtggg gagggcatga
     1621 gcttaactgg agattcccga aaaggagcga gatagacatg cgctggtctg atgtcggaag
     1681 gatgtacact tcaggtgata ttcgcgaagt agagaag
//
LOCUS       DATGBN010000037         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_352384, whole genome shotgun sequence.
ACCESSION   DATGBN010000037 DATGBN010000000
VERSION     DATGBN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352384"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..850)
                     /gene="moeB"
                     /locus_tag="VKL21_00790"
     CDS             complement(41..850)
                     /gene="moeB"
                     /locus_tag="VKL21_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HMB44335.1"
                     /translation="MLGGFNEEQIRRYSRHIILPEVGGKGQKKLLSSKVLCIGAGGLG
                     APIIEYLAAAGIGTLGIIDDDTVDLSNLQRQVIHGGNVGKPKAESAKQFVNNLNPDIE
                     VKTYVQRLNANNVIDIFKEYDIIVDGSDNFATRYLVNDACVITNKPLSHGSIYRFEGQ
                     VTTIIPHKGPCYRCLFEHAPPPGMVPSCQEAGVLGVLPGIIGVIQATEVVKYLLGIGE
                     LLVGRLIYYDALNMTFQEIKMRWNKNCPICGEKPRITSIQEEIYGDSCRIW"
     gene            1168..1818
                     /gene="pyrF"
                     /locus_tag="VKL21_00795"
     CDS             1168..1818
                     /gene="pyrF"
                     /locus_tag="VKL21_00795"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMB44336.1"
                     /translation="MRNNTQLILALDVTDRKNAVRISKETADHVDAIKIGYPLVLGAG
                     LGIINDICKFAPVIADFKVADIPNTDRLICSQAFEAGASAVIVHGFTGRDSVSECVKT
                     AKDFHGDIFVVTEMSHPGAVEFMQPKALELASIAVECKASGVVAPATRPQRLKQIRDV
                     VGKLTIISPGVGAQGGSAADAIRAGADHVIVGRSIYGSKTPGIEAEKIVKEIEKCR"
     gene            2432..2593
                     /locus_tag="VKL21_00800"
     CDS             2432..2593
                     /locus_tag="VKL21_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44337.1"
                     /translation="MDGEKEWPGELINMILNLFGIEMKIFENSEEQKREKNRKEVIDS
                     WKFWKQGGI"
     gene            complement(2598..4049)
                     /locus_tag="VKL21_00805"
     CDS             complement(2598..4049)
                     /locus_tag="VKL21_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44338.1"
                     /translation="MSIIFLFWFNKHKDPGISKIIIFSAILFTLLNSYWIIPVLTAKD
                     TVLDNINDEDFKVFAPKIESFSDIFNIASMYGFWREDYIYAKDFIPYWQLLSEFIILL
                     AIYGFISYYKDPKIGIFARAFGVIGILGFILATGINGPFGGINHWLFENTILKGFRDS
                     HKFVAMIVLAYAALGGLGIKKIKNEYDSNYAVPKRKIVKFLLLAIICISLITPLVYSF
                     TFFNGFAGQIKPTDYPEDWYETNNYINEDKQDFKILFFPWHGYMDFKWINNTNKRIAN
                     PARSFFDKEVISGTNAEIGDLYREVNTPEQVYIDSLLEKRDNITDFGELISILNVKYV
                     ILTGESDYKKYFFIFNQTDLELVKQTENFYIFKNKFDVKKIYQTDDIDNISAEKMELG
                     YEQVNPVRYGLQENISKKYMVFTEAFSIDWKLDGRAPLKAYGVVNAFENSGKEIRFER
                     FYRINLPAYFISILTFIGLIAMYPGLDKRKNSN"
     gene            complement(4567..>4851)
                     /locus_tag="VKL21_00810"
     CDS             complement(4567..>4851)
                     /locus_tag="VKL21_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44339.1"
                     /translation="AAAILIAYNTDDKLLQPGIFVALLLIIVIRRNESRIPTVYALIM
                     FLISAFLLAFQVETAANKTAILGYYLLCAGVIAQFIEYIRNPEVDDHKNE"
BASE COUNT         1537 a          919 c          891 g         1504 t
ORIGIN      
        1 gacaaacctc ccccttcagg tcaagttcga tcgtcatatt tcaccatatt ctgcatgaat
       61 caccatatat ctcttcctgt atcgaggtta tcctgggttt ttccccgcaa atcgggcagt
      121 tcttgttcca tctcatcttg atctcctgga aagtcatgtt aagcgcatca taataaatga
      181 gacgccccac aagcagttca ccaattccga gaagatattt aactacttcg gttgcctgta
      241 tgacaccgat tatccccgga agaactccaa gtacaccggc ttcctggcag ctgggcacca
      301 ttcccggagg aggcgcgtgt tcaaataagc acctgtagca tggtcctttg tgtgggatta
      361 tggtcgttac ctgcccctcg aacctgtaga tgctgccgtg agatagtggt ttatttgtga
      421 taacacatgc atcgttcaca agatatcttg ttgcaaaatt atcagaaccg tcaacaatga
      481 tgtcatattc tttgaaaata tcgataacat tattggcatt cagccgctga acgtatgttt
      541 tcacttcaat atcaggatta agattgttaa caaattgttt tgcggattca gctttgggct
      601 ttcccacatt cccgccgtgg attacctgtc tctggagatt gcttagatcc acagtgtcat
      661 catcgataat accaagagtc ccgatgcctg ccgccgccag gtattcgatt atcggagcgc
      721 caagaccgcc tgccccaatg cataatacct ttgacgataa cagttttttc tgtccttttc
      781 cacctacttc cggcaatatg atatgacgcg agtatcttct tatttgttct tcattgaatc
      841 cgcctaacat tgttttatca ccatattatt actgtgggta ctatttacac ccataaagta
      901 ttaccagcat aaataatttt tggtgatatc tatcccaaaa aaacctttgt tgagtctgca
      961 tttttaggaa tagacaaact aaatatacca tcaaaataag atatgacttt ttaagtttgc
     1021 atgatcaaaa atgataccat caaagtctct catcttaacc atgcgacaga ggataatgag
     1081 caggaaaatc ttatacatgt catccaaaac atgttcgaat tacgggaacg ttcacagttg
     1141 tagcgtgagg tagttcacat ggagtcgatg cgtaataaca ctcaattgat cctggctctt
     1201 gatgttactg acaggaagaa cgctgtcagg atatcaaagg agactgctga tcatgtagat
     1261 gcaattaaaa tcgggtatcc gcttgtgctg ggcgcaggat tggggatcat caatgatatc
     1321 tgcaaattcg cacctgtcat tgccgatttc aaagttgcgg acatcccgaa tacagaccgt
     1381 cttatatgca gccaggcctt tgaggcgggc gcaagcgcgg tcatagttca tgggtttaca
     1441 gggcgggaca gcgtttcgga atgtgtcaaa acagcgaaag attttcatgg ggatatattt
     1501 gtggtcaccg aaatgagcca tcccggcgct gtcgagttca tgcagcccaa agctcttgaa
     1561 cttgcatcta ttgcagtaga atgcaaagct tcaggtgttg ttgcacctgc aacacgcccc
     1621 caaagactaa aacagatacg tgatgttgtg ggcaagctta caatcatttc accaggcgtt
     1681 ggcgcgcagg gcggcagcgc cgcagatgcg atacgcgcag gcgcagatca tgttatcgtt
     1741 ggaaggagta tatacggatc aaagactccc gggatcgaag ctgaaaagat agtgaaagag
     1801 atagaaaagt gccgatgatg agaaaaccgg agctgaaatg tgatgagccc tatgagtact
     1861 gatggcacca ataaatcatt tgataatatg aaggttggca ggattatggc atgatacctg
     1921 ccactgcttt tcagtattct catcaattga taattgccga aaagactaac gtccttcagg
     1981 gtaaatattt gttaatgaag aaagatttga agagaatcaa gggcggatat agaccacaga
     2041 aacatttcca gaatttatat catattctac agacattcgc tgtctttaga ctatatattg
     2101 cacaggtatg gaaaaaatca tttcagttca agagagccca aaactatcat atcaggttat
     2161 ctgcaggctc agttgaagct gagcccttca tccacccgga tagaaatcct taaggcacct
     2221 aatatatcaa agaaatattg tgcaattttt gcatatttat ggtatatgtg ggaaactata
     2281 aataattaaa cctcaacaga ggttgaagca aaccaaagct tctttaatca ggttaatgct
     2341 taatcgtgta aatgcaaatg ttgcgtattt gcacagcatt taaatactaa tctggttgta
     2401 tgtattaatt gtgattaagt tgtacaataa tatggatggc gagaaagaat ggccaggaga
     2461 attaataaat atgatattaa atttatttgg aattgagatg aaaatatttg aaaattcaga
     2521 agaacagaaa cgagagaaga atcgaaaaga agtaattgat tcatggaaat tctggaaaca
     2581 aggtggaata taataattta attactgttc tttctcttgt ccaatcccgg atacatcgct
     2641 attaatccaa tgaatgtcag aattgaaatg aaataggctg gaagattgat tctgtaaaat
     2701 cgttcaaatc gtatctcttt accactattt tcaaaagcat ttactacccc ataagccttt
     2761 aaaggcgctc gtccatctaa tttccaatct atggaaaatg cctccgtaaa gaccatatac
     2821 ttcttactta tgttttcctg caacccatat ctaacaggat ttacctgttc ataacccaat
     2881 tccatctttt cagcgctgat attatcgata tcatcggttt gatatatttt ttttacatcg
     2941 aatttatttt taaatatgta aaaattttca gtctgcttca ccaattccag gtcggtctgg
     3001 ttaaatatga aaaaatattt cttataatct gattcacctg tcaaaataac atatttgaca
     3061 tttaatatag atattaattc accgaaatct gtaatattgt cccttttttc taataaagaa
     3121 tctatataaa cctgctctgg tgtatttact tccctgtaaa gatctcctat ctcggcattt
     3181 gtgccgctta ttacttcttt atcaaagaaa gatctggcag gatttgctat tctcttattt
     3241 gtattattta tccatttgaa atccatatat ccgtgccatg ggaaaaaaag aattttaaaa
     3301 tcttgtttat cttcatttat ataattattt gtctcatacc aatcctcagg gtagtctgtt
     3361 ggctttatct gtcctgcaaa tccattgaag aatgtgaaag agtatactaa aggagtaatt
     3421 aaagaaatgc aaatgatcgc caataataaa aatttgacga tctttctttt tggtaccgca
     3481 taattactgt catattcatt ttttatcttc tttatcccca atcccccaag cgctgcatac
     3541 gccagaacaa tcatcgcaac aaacttatgt gaatccctga atcctttcaa tatggtattt
     3601 tcaaataacc agtggtttat gcctccgaat ggtccattta tccctgtggc taaaatgaat
     3661 cccagaattc ctatcacacc aaaagctctt gcaaagatcc caatttttgg atctttataa
     3721 tatgaaataa agccatagat cgctaataat attatgaatt ctgataataa ttgccagtat
     3781 ggaatgaaat cttttgcata tatgtaatct tctctccaga agccatacat gcttgcgata
     3841 ttgaaaatat ctgagaaact ttcgattttc ggggcaaata ctttgaaatc ttcatcgtta
     3901 atattgtcca gcactgtatc ttttgccgtt aatactggta tgatccagta tgaattgagg
     3961 agagtaaata gaattgcgga gaatataatg atttttgaaa ttccaggatc tttgtgtttg
     4021 ttgaaccaga ataaaaaaat tatgctcatt acaattaaag ctaataccaa taaatggaga
     4081 ctgaaactca caaatgtcaa aaggaatatg aatttgatca tttccttctt ttcttttttc
     4141 tctaataagt caataaatgt gattaataat agaggaagcg ccgcatatga taaaataatg
     4201 tacagatgac cggccaatat tctaatatat atatatgggt ttatcatata caatagaccg
     4261 gcataaagac ctgagagtcg tgattgggag attttacaaa acaggttata catcgaatat
     4321 cctgaaagaa gcattgcaga aaacaaatat atcctctgta ttatccagga tgaggaatct
     4381 attattgggg aaggatatga aaaataaaat ttagcaagcg aatttttcac aggaaaaatc
     4441 atatcaagcg ccaatatata attattttca aaattgagcc agattaataa tgagattgat
     4501 aaaaatagga ttattgggaa atgtcgaatt atccttttcc tcactgtatc gattaaagct
     4561 tcagatttat tcatttttat gatcatcaac ttcaggattt cttatatatt ctataaactg
     4621 ggctattacc ccagcacaca aaagataata cccaagtatt gcagtcttat ttgcagcagt
     4681 ttctacctgg aatgcaagca gaaaggcgga aattaaaaac ataattagag cataaacagt
     4741 aggtattctc gattcattcc ttcttatcac gattattaga agaagcgcga cgaagatgcc
     4801 aggttggagc agtttgtcgt ctgtattata tgcaataagt atggctgctg c
//
LOCUS       DATGBN010000038         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_274580, whole genome shotgun sequence.
ACCESSION   DATGBN010000038 DATGBN010000000
VERSION     DATGBN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274580"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..408)
                     /locus_tag="VKL21_00815"
     CDS             complement(169..408)
                     /locus_tag="VKL21_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44340.1"
                     /translation="MKTNEDIQELNKQALLYSKDLEAIIQEMHRVIIGQESVIEKLIL
                     ALVADGHMNCLKPRLTASCSRYCSIIPHRCRNRKL"
     gene            556..819
                     /locus_tag="VKL21_00820"
     CDS             556..819
                     /locus_tag="VKL21_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44341.1"
                     /translation="MVSYLIDNLIGAVRFFVGLILIIIAMRAFLKTRTSDMFYLTMGF
                     ALITIGNLFSTIYYVDDVRMDKLLANVFDILGLIALIIAVRKS"
     gene            825..1190
                     /locus_tag="VKL21_00825"
     CDS             825..1190
                     /locus_tag="VKL21_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMB44342.1"
                     /translation="MNQDPGDEEIVFELLANKYSRRILSLTSTKECSASQLSQELEIP
                     LATVYRKLKFMENSGLLRNVKTIINLSGNEEKYYRCAIREATVHIHNGIISLDFKKED
                     QSDKIIRLWKRLAHANDNK"
     gene            1570..>3303
                     /locus_tag="VKL21_00830"
     CDS             1570..>3303
                     /locus_tag="VKL21_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMB44343.1"
                     /translation="MNIKNMRVLLMGIALIISIFIPSVGANPGLKESKDYRNFADQNI
                     EFGDSKIQSNLLEAIENKKDEEKEIIIVLKKDRNKNNVYNFLSQGTLQKTLPDKTKYH
                     KLANAFSAKVKADTIKDIADLDDVEKVYEDFKVTVALYDSVPLIKADSLWSKYDGTGV
                     KVAVLDTGVDSNHPDLKGKIVDKVSFAKYESPEDGNGHGTHVAGIIAGSGASSGGKYK
                     GVAPGASLMNIKVLNNMGYGPASSIMEGIEYAVDNGADIISMSLGGSIWPPDGTDPLS
                     ITANAAVDAGVVVIVAAGNGGVPFQIGVPAAAEKVIAVGASTKDEKMAEYSSQGPTWD
                     HRIKPEVVAPGGASPISFDPAGLGIVSTKAAGSLLDLMNPDYGVDKYYMALSGTSMAT
                     PHVSGVAALLLQAYPKLTPEQIKQRLMNTGVDLGYDPINQGAGRIDSVSAVNNTVNII
                     PVSLSYIMNPGTNRKETIKITNNGKKEITLSLISTGDLNIEFSNKTLPIKPGETESII
                     AMIGIPAGLSAGVHAGNIMLYDGDTLTARIPVLEDTPITFVGGKSELHDKIEIRSLKD
                     YRRGTNYYYFDV"
BASE COUNT         1050 a          597 c          687 g          969 t
ORIGIN      
        1 tatcaagacc gtattgccgg gggtctcttg ttgcctgaac gatatctgtg atatattttg
       61 agattttttc atccatatag atgcggtgat tgaaattctg gatctcgatt atcctgggtg
      121 gggaaatgat ctttcctatc ctggacaatc ccacacctgc gaccatgttc ataatttcct
      181 gttcctgcat ctgtgtggga taatcgagca atatcttgaa catgaagcgg tcaacctggg
      241 cttcaggcag ttcatatgtc catctgcaac gagtgcaagg atgagttttt caataacgct
      301 ttcctgtccg attattaccc ggtgcatttc ctggataatc gcttccaggt cttttgaata
      361 aagcagcgcc tgcttgttga gttcctgtat atcttcgttc gttttcatca cctcaataag
      421 tctcttcgag tatccgttct tatttgtgaa atatttattt tttactatgt tcttgaatat
      481 taaatgatag tgtccgttct cagaaattga gaatgacaga agttattatc tttgaatcaa
      541 taattaatgg atattatggt ttcatacttg attgacaacc ttatcggtgc ggttcgtttt
      601 tttgtagggc tcatattgat tattattgca atgcgggcgt ttttgaaaac aaggacttct
      661 gatatgttct atcttacaat gggttttgca ctcatcacga tcggaaatct attttcaacc
      721 atttattatg tcgatgatgt gcgtatggac aaattgcttg caaatgtttt tgatatactt
      781 ggattaattg cattgataat agcagtaaga aaaagctgac tgaaatgaac caggatccgg
      841 gcgatgaaga gatagttttt gagctgcttg caaacaagta ctctcgaagg atattgtccc
      901 tgacatcaac aaaggaatgt tcggcaagcc aattgagcca ggagcttgaa atacctcttg
      961 caactgtata tagaaagctt aaattcatgg agaattcggg tcttttgcgg aatgttaaaa
     1021 ccattataaa tctttcagga aacgaggaaa aatattatcg ttgtgcgatc cgtgaggcca
     1081 cagtacatat tcacaatgga ataatctctt tggactttaa gaaggaagac caaagcgata
     1141 agataatcag attatggaaa aggcttgccc atgcgaatga caataaataa aataaatgcc
     1201 cttcttttat tctcaaatct tgagaatggg ctattccatt tatctttata aaaagcaatg
     1261 tgagtaatgg aggacagcag catgaacaac atttctttac cccttcacca cctattatca
     1321 tgctgccatc ctatcctcca tttttttaag tattcaacag tttcttcatc aaacaaaatg
     1381 tgagcaatat atcactatat taaaaaaaaa caatattgat aagatgtatc tttcaatgag
     1441 cattttttaa ataggaaagt atttatttga gcaaagccaa accggacaaa aagtatccaa
     1501 gttgtaataa atattgactt gccgaaagaa aattaattca gcttgtttat tttacttggt
     1561 ggtggaaata tgaatattaa aaatatgaga gttttgttaa tgggaattgc tttaattata
     1621 agtatcttta tcccgtcagt gggagcaaac cctggactca aagaatcgaa agactatcgc
     1681 aattttgcag atcaaaatat tgaattcggg gactcaaaaa tccagagtaa cctcctggaa
     1741 gcaatagaaa ataaaaaaga cgaagaaaaa gaaataatta ttgtattaaa aaaagatagg
     1801 aacaaaaaca atgtatataa ttttctttcc cagggtacac tacaaaaaac cctgcctgac
     1861 aaaaccaaat atcataaatt ggcgaatgct ttttctgcaa aagtaaaagc tgatactatc
     1921 aaagatattg ctgaccttga tgatgttgaa aaggtatacg aggatttcaa agtaactgtt
     1981 gccctttatg acagtgtccc tttgatcaaa gcagattctt tatggtctaa atacgatgga
     2041 accggagtaa aagtagcagt gctcgatact ggtgttgatt caaaccatcc cgacctcaaa
     2101 gggaagatcg tcgataaggt ttcattcgca aagtatgaat caccggaaga cggaaatggc
     2161 catggcactc atgtcgcagg catcatagca ggcagtggag catcttccgg cgggaaatat
     2221 aaaggagtgg cgcctggcgc atcattaatg aatatcaagg ttctcaataa tatgggatat
     2281 ggacctgcat catccattat ggaaggaatc gagtatgctg ttgataatgg cgcagacata
     2341 atttccatga gccttggagg gagtatctgg cctcctgatg gcactgaccc tctatccata
     2401 acagctaacg ccgcagtgga tgcgggtgtg gttgtcatag ttgcagcagg aaatggtgga
     2461 gtgcctttcc aaattggcgt tcctgcagcc gcagagaaag taattgctgt aggtgcaagc
     2521 acaaaagatg aaaagatggc tgagtacagt tcccaggggc ctacctggga tcacaggatc
     2581 aaacctgaag tggtcgctcc tggcggtgca tcacccatat cttttgatcc ggccggatta
     2641 ggtatcgttt caaccaaagc agccggatca ttgcttgatc taatgaatcc tgattatgga
     2701 gttgataaat attatatggc attatcagga acatccatgg caacgcctca tgtttcagga
     2761 gttgcggccc ttttattgca ggcatacccc aaattgacgc ctgaacagat aaaacagaga
     2821 ttgatgaata caggtgtgga tcttggatac gatcctataa atcagggcgc cggtagaatc
     2881 gattcggtat cagcagtgaa caatacagta aatataatcc ctgtcagttt aagttatata
     2941 atgaatccag gtacaaacag gaaggagacc attaaaatta ctaacaatgg aaaaaaggaa
     3001 ataacacttt ccttaataag cacaggagat ctaaatattg aattctcaaa taagactttg
     3061 ccaattaaac ctggcgaaac agaaagcata atcgctatga tcgggatacc tgctggctta
     3121 tctgccgggg tgcatgctgg taatattatg ctatatgatg gagataccct gacagcaaga
     3181 atcccggtgc tggaagatac acccataacc ttcgttgggg gcaagtccga actccatgat
     3241 aaaattgaaa taagatcatt aaaggattat agaagaggta ccaattatta ttattttgat
     3301 gtg
//
LOCUS       DATGBN010000039         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_235989, whole genome shotgun sequence.
ACCESSION   DATGBN010000039 DATGBN010000000
VERSION     DATGBN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235989"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VKL21_00835"
     CDS             <1..1085
                     /locus_tag="VKL21_00835"
                     /inference="COORDINATES: protein motif:HMM:NF020078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HMB44344.1"
                     /translation="LAMELELKTQNDAQKVRELVIETVEKMAVFSEENTNPGIVFIEN
                     PTPGLKNDLSKFSLRAVQDVLEIREAKELLIRHGISHKGYKNERGLIGALAAAGFSLC
                     GFSDHTFELIAYREQQKWGSLREIDPVTVWSADAATHPDTWDTVDIDNNRIVFAPHSP
                     DPILFGIRGKNEEAVRKAFSMIKREPVERHIVYKTNQNTDMHLINAKIHEVSNDRSYI
                     LSGMVSKSPRTIMGGHIIFGLSENGAHIECTAFEPTKGFRRIIRELMIGDEVTVFGSV
                     KEETLNLEKIKVIRLNMAKLRNPVCCEKRMKSMGKGQGKRCEKCGAIKKERVIENLPR
                     NISEGFYEVPPCARRHLSKPLIRYPF"
     gene            1229..2155
                     /gene="cbiM"
                     /locus_tag="VKL21_00840"
     CDS             1229..2155
                     /gene="cbiM"
                     /locus_tag="VKL21_00840"
                     /inference="COORDINATES: protein motif:HMM:NF004902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HMB44345.1"
                     /translation="MHIPDTFIPLGQAAFYWIISILFIARAVKWARKDMKENMIPLFG
                     VLAAGIFVLQTINIAANLIVPIPMLTGVSWHIVGAALAAIIFASPWAAVLLMTMVLAI
                     QSLFGDGGITVMGANILNMGIIGGFLGYYTFIGLSKLSVKRPLAMFAGAFISMLMPGI
                     ALAFELWLAGTFPLKQGVLLMGTFQGVAGIGEGFITLIVFGAISKARPDIVNEETHAK
                     ESTAKVAAVGIAALLGLAVAAPFLASNNPDGLEQTASLVLTNEIGSRITLLFSDYAIP
                     GMGKAGEVAAIIIGLAAILLIWTGISRIIKKA"
     gene            2157..2981
                     /locus_tag="VKL21_00845"
     CDS             2157..2981
                     /locus_tag="VKL21_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB44346.1"
                     /translation="MNIIETRNLTHVYRGKITALDAINFTAKPGERIAIIGANGAGKS
                     TLFKHFNGILKPTSGEILIKGKSISSENILDVRKTVGVVFQDPDDQIFAPTVKQDVAF
                     GPMNLGLGIEEIEKRVRESLETVRLTGFEDRAPHHLSTGEKKKVAIAGILAMQPQVLV
                     LDEPTAGLDPGGAIRLIKLINGMNRYLGITTIVSTHEVDIVPMVADRVCIMSKGKIIG
                     DGTPLEIFSSPDLIKKTHLRLPMVAQLMEMLQEEGVPVKVKFTLEEARDELLKVVK"
BASE COUNT          927 a          623 c          726 g          769 t
ORIGIN      
        1 cattagctat ggaacttgaa cttaagaccc aaaatgacgc acaaaaagtc agggaactgg
       61 taattgaaac ggtcgaaaaa atggcagtct tttctgagga aaacacaaat cctggaatcg
      121 tttttataga gaatcccaca cctggtttga aaaatgacct ttctaaattc tccctccggg
      181 ccgttcagga tgttctggaa attcgtgaag ctaaggagtt attaattcgt catggaattt
      241 cccataaggg gtataagaac gaaagggggt taataggggc tctcgcagca gccggttttt
      301 ccttatgcgg tttttctgat cacacttttg agcttattgc ctacagggaa caacaaaaat
      361 ggggctcatt gcgtgaaata gacccggtaa cagtatggtc agctgacgcg gcaacacatc
      421 ctgatacatg ggatacggta gatatcgata ataaccgaat agtatttgca ccgcactcgc
      481 ctgaccccat tcttttcggg atacggggga aaaacgaaga ggctgttaga aaggcatttt
      541 cgatgatcaa acgcgaacct gttgaaaggc acattgttta taaaaccaac cagaataccg
      601 atatgcatct tataaatgca aaaatacatg aagtcagcaa tgatcgctca tatattcttt
      661 caggtatggt aagcaaatcc ccaagaacga taatgggagg gcatattatt tttggacttt
      721 cagaaaacgg cgcccacatc gagtgcacag cctttgaacc tacgaaaggt ttcaggcgta
      781 ttatcaggga acttatgatc ggggatgaag tgacagtatt cggaagcgtt aaggaagaga
      841 ctttgaacct tgaaaaaatc aaagtcattc ggctgaatat ggccaaattg cgaaaccctg
      901 tgtgctgcga aaaacgaatg aaatccatgg gaaaaggaca gggtaaaaga tgcgagaaat
      961 gcggtgcaat aaaaaaagaa cgcgtgattg agaacctgcc gagaaatatt tccgagggat
     1021 tctatgaagt ccctccatgc gcaaggcgcc acctgtcaaa gccgcttata agatatcctt
     1081 tttaagcatc cctatcgacc gtaacctaaa tatatccgga tatagtataa cattgattta
     1141 gtcagtatta ctaattcata taatcgtaat attatgtgtg tgtgaaataa caaaatatta
     1201 ataatagtat ttcaggagta aagataatat gcatataccg gacacattta tacctcttgg
     1261 gcaggctgct ttctactgga tcatttcgat attattcatc gcacgggcgg taaaatgggc
     1321 gcgaaaggac atgaaagaga acatgatccc cctttttggc gtgcttgcgg cgggcatttt
     1381 tgttctccag acaattaata tagctgcaaa cctcatagtt cccataccaa tgctcactgg
     1441 cgtcagctgg catatagtgg gagctgcgct tgctgcaata atatttgcca gtccctgggc
     1501 ggcagtgctg ctcatgacca tggtgcttgc tatacagtca cttttcgggg acggcggtat
     1561 cacagtaatg ggagcgaaca tattgaacat gggaattatt ggaggattcc tggggtatta
     1621 cacattcatc ggtttgagca aactttcggt aaaacgtcct ttggcaatgt ttgcaggggc
     1681 gttcataagt atgttgatgc ctggaattgc ccttgcgttt gaactctggc tggcaggcac
     1741 tttcccgctc aagcagggcg ttcttctcat gggaacattc cagggagttg ccggaattgg
     1801 cgaagggttc ataaccttga tcgtatttgg cgcaatatca aaagcaaggc cggatatcgt
     1861 taatgaagag acacacgcaa aagaaagcac agcaaaggtt gcagctgtgg gaattgcggc
     1921 attgcttgga cttgctgttg cagcgccgtt tcttgcttca aataatccgg acgggcttga
     1981 gcagactgca agcctggtcc taacaaatga aattggcagc aggataacac ttcttttttc
     2041 agattatgcc ataccaggaa tgggaaaagc aggagaagtg gcagctatca taattggatt
     2101 agcggcaata ctgttgatct ggacagggat ctcaagaatt attaaaaagg cctgatatga
     2161 acattattga aacacgcaat ctcacgcatg tataccgcgg aaagatcact gccctggatg
     2221 cgatcaattt cacagcaaaa cccggtgaaa gaattgcaat aataggagcc aacggcgcag
     2281 ggaaatcaac gcttttcaag cactttaatg ggatactcaa gcccacaagc ggggagatcc
     2341 tgatcaaagg caaatcaata agcagtgaga atatccttga tgtcaggaaa acagttggtg
     2401 ttgttttcca ggaccctgat gaccagattt ttgctcccac cgtaaaacag gatgtggcat
     2461 ttggtcctat gaaccttggt ttaggtattg aggaaatcga aaaacgtgtc cgcgagtcac
     2521 ttgaaaccgt aagactgacc ggttttgaag atcgagcgcc ccatcatctc agcacaggtg
     2581 agaaaaaaaa agtcgcaatc gccggcatac ttgcaatgca gcctcaggtt cttgttcttg
     2641 atgagcccac agcagggctt gacccgggtg gagctatacg cctgatcaaa cttatcaatg
     2701 gaatgaacag gtatcttggg ataacaacca ttgtctcaac tcatgaagtt gatatagtac
     2761 ccatggtagc ggatagggtt tgcatcatgt caaagggtaa gataattggg gatggcacac
     2821 cccttgaaat cttctcatcc cctgacctca taaagaaaac ccatctccgt ctcccaatgg
     2881 tcgcacagct catggagatg ctccaggaag aaggtgtgcc cgtgaaagta aaattcactc
     2941 ttgaagaagc aagggatgaa ctattaaagg tcgtgaaata aaatgcatgt ttccattacg
     3001 gaaatggagc gggagacata taagaaatcc ccgatccatg gaatt
//
LOCUS       DATGBN010000040         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_507936, whole genome shotgun sequence.
ACCESSION   DATGBN010000040 DATGBN010000000
VERSION     DATGBN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507936"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..620)
                     /locus_tag="VKL21_00850"
     CDS             complement(39..620)
                     /locus_tag="VKL21_00850"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMB44347.1"
                     /translation="MQIGTDIRKASEIIKKNGIVIYPTETVYGIGANIFSIAALEKVF
                     AIKKRDKDKPVSVAVSDLKMMESLVYIGEKEKRFIEKFLPGPVTVVLKKKEIVNDMLT
                     SGSELVGIRFPAHKTTIELIELAGVPITSTSANFSGEAPPRRVGDIKISADYIIDGGE
                     CWGEPSTVVDLVNRKIIRKGAQFDEVKTFLEIF"
     gene            complement(720..884)
                     /locus_tag="VKL21_00855"
     CDS             complement(720..884)
                     /locus_tag="VKL21_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44348.1"
                     /translation="MSIVKDLIFNEYSGKFQKDETTDKSTINTFGQHYLNGAGVATPD
                     PNGCAASGGM"
     gene            complement(925..1161)
                     /locus_tag="VKL21_00860"
     CDS             complement(925..1161)
                     /locus_tag="VKL21_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB44349.1"
                     /translation="MAKDKKLMQSDICNGCGICVLVCPTNIKLGKADDFDMNNSKLAI
                     SVTNGAAVIDPDACIACGICTRNCPVTSLTIVPA"
     gene            complement(1200..2297)
                     /locus_tag="VKL21_00865"
     CDS             complement(1200..2297)
                     /locus_tag="VKL21_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB44350.1"
                     /translation="MLNVLIIGVGQCGNRILDSINKEAFGGGSLAKYYGSQRFKSHVE
                     TLAINTAVNDLKELKHTKARDRIQVPHLHGVGANRNVGKKTFEENRELLLRIIEERGD
                     FDVAFVMASSSGGTGSSFAPLLIDAMKERYKYNVYGIIVLPFREEGSIYLQNSIFCVK
                     EIMDGNGDGTILVDNQYLKHLGKDIKNAYDEINSTVARRFLFLLKALDSEMMMVTDLG
                     DFKTVMSGGTKLVTMGFGKGDKNMPIKSVIQHSISYAGLLFKLEPYKESNRAMIIIEG
                     DPKYLNINDITSEVEKLSSEIGQIFKGILLRNGEMPRVLTLLSISTSAELDRLFEIGI
                     DAVHKEKDKKEQTSGLNQEIINRLEGLEPQY"
     gene            complement(2305..3462)
                     /locus_tag="VKL21_00870"
     CDS             complement(2305..3462)
                     /locus_tag="VKL21_00870"
                     /inference="COORDINATES: protein motif:HMM:NF017561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HMB44351.1"
                     /translation="MIPKRKNDYTLLKELSFLILILLFFSMPVNAEKITQYVYLENNT
                     GLNFENLNYKVEVFEINEYSGYIRIKEISGKSSDNHTLTEGDPLDLFNTITLNLSSII
                     QKTAKIAIEYPDNWPKPIEYNVEISVAEEKIPNIVLTKSADKTTLNKGDVIEFKINLE
                     NTGNGTATNLSLDEKLPQGFARAPGSSFPPALQDELKAGERLELLFALKAVESGTYDI
                     EPTTIKYGSNTSRSNSLSITVLDEKKEKSNLSTVITLDKNNILTGESVKATVKITNNG
                     NVSAESILIEGAIPKGMEVKEGDLRQVFKKIDAGESEEYFATLKAVEPGNYSIILKTS
                     YSDDSTGFSSDSDSIIVKENEKDYLYILVPAIIIIAVIALFIIRRHREYSF"
     gene            complement(3533..>4366)
                     /locus_tag="VKL21_00875"
     CDS             complement(3533..>4366)
                     /locus_tag="VKL21_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HMB44352.1"
                     /translation="YTSRKLQSQIYIDSVNWFLLFSVLMVMILFKESHSLAAAYGLAV
                     TGTMTLTGIMMTWIFYLKDNRLKTSIAALVTFVDMAFLLSSLHKIPSGGYWSIILALI
                     PFSVIMIYTSGQKKLSKTLSPMVLDDFLKEYNQVYNNMPKIKGTALFFAKEIIKIPPY
                     MANTMFNNNIIYEDNIIVSLIKTENPFGISGSLKSDLADGLRVFEIYMGYMEVVDVET
                     LLKENGIEEKTIFYGLEDIVSDNVIWRIYSMIKRLTPAFVQFYKLPPHKLHGVLSRIE
                     M"
BASE COUNT         1223 a          935 c          777 g         1431 t
ORIGIN      
        1 gttaaattaa agatagcgta attttgctgc ccctggcgtt aaaaaatctc caaaaaagtt
       61 ttcacctcat caaattgtgc gccctttctt atgatcttcc tgttaacaag gtcaaccacc
      121 gtagaaggct cgccccagca ttccccgcca tcaatgatat aatccgcgct gatctttata
      181 tcacccactc ttcgcggcgg ggcttcccct gaaaagttgg cactcgtaga tgttatcggc
      241 accccggcaa gttctatgag ttcgatcgta gttttgtgag ccgggaaacg tatacctacc
      301 aattccgaac ccgaagtgag catatcgttg actatttcct tctttttcag taccacagtt
      361 acgggaccgg ggagaaactt ttcaataaac cgtttctcct tttccccgat atacacaaga
      421 gattccatca ttttaagatc agatacagca acagaaaccg gtttatcttt atcccgcttc
      481 tttatggcga atactttttc aagggcagcg atcgagaata tatttgcccc gatgccatat
      541 actgtttccg tgggatagat tacgataccg tttttcttga tgatttccga agctttcctg
      601 atatccgtac ctatctgcat tgatgagtct cacatctaaa aatattacta ctgataaggg
      661 aatgagtgtt caaacaattt aagaaatctg cgttcgtgta tacaacaggt gatattatgt
      721 tacatcccgc cagaggcggc gcatccgttg gggtctgggg tcgcaacccc agcgccgtta
      781 agataatgtt gaccgaaagt gttaatggta ctcttatctg tggtttcgtc cttttgaaat
      841 ttgcctgaat attcattgaa aatcaggtcc ttaactatag acataattta accgcaaagc
      901 ggatacaaaa aaaatctgcg gtcttcacgc cggaacgatc gtcagggatg ttaccgggca
      961 gttgcgggtg catatcccac aggcgatgca tgcatcagga tcgatcactg ctgccccgtt
     1021 ggtcactgag atcgcaagtt ttgagttatt catgtcaaaa tcatcagctt tacccagttt
     1081 gatatttgtg gggcagacca acacacatat tccacatcca ttacaaatat ccgattgcat
     1141 taatttcttg tcctttgcca taatatatga tacctcttag agtgatatga acatttcgat
     1201 taatactggg gttccaatcc ctcgagccta tttatgatct cctggtttaa tcctgatgtc
     1261 tgttcttttt tatccttttc tttgtgtacc gcatcaatac cgatttcaaa caatctgtcc
     1321 agttcagcag aggtacttat cgaaagcaag gtcagcaccc ttggcatttc tccgttgcgt
     1381 aagagaatac ccttgaatat ctgtcctatt tcgctggata atttctccac ctcgcttgta
     1441 atatcattga tgttcaggta tttcggatcc ccttcaatta ttatcatcgc cctgtttgat
     1501 tccttataag gctcaagctt aaaaagaaga cctgcatatg aaatactgtg ctggataaca
     1561 gatttaatgg gcatattttt atcgcctttc ccaaatccca tagtaacaag ctttgttccc
     1621 ccggacatga cagttttaaa atcccccagg tcggtcacca tcatcatttc gctgtcaagc
     1681 gccttcaata gaaagagaaa cctcctggcg acagtcgaat ttatttcatc atatgcattt
     1741 tttatatcct ttcccagatg tttcagatat tgattatcca caagtattgt accgtcgcca
     1801 tttccatcca ttatttcctt tacgcaaaag atggaatttt gcagatagat cgaaccttcc
     1861 tccctgaatg gaaggactat gatcccatac acattatatt tatatcgttc tttcatcgca
     1921 tcgatcaaaa ggggcgcaaa agaagaacct gttccacctg atgaggaagc cataacaaag
     1981 gcgacatcaa aatcaccgcg ctcctcgata atacgaagca agagttctct attttcctca
     2041 aaagtttttt tccccacatt cctgttcgct cccactccat gcagatgagg cacctggatc
     2101 ctgtctcttg cttttgtatg tttcaattct ttaaggtcgt tcacagctgt gttgatcgcg
     2161 agtgtttcaa catggctttt aaatctctgg cttccgtaat actttgccag ggagcccccg
     2221 ccaaacgctt ccttgttaat ggaatcaagg atcctgttgc cgcattgtcc aactccgatg
     2281 ataagtacat tcagcatatt tcctctagaa tgaatattcc ctatgtctcc tgataataaa
     2341 taacgctatt acggctatta ttattattgc gggaacaagg atatacagat aatctttttc
     2401 attttctttc acaataatgg aatccgaatc ggaagaaaaa cctgttgaat catcagaata
     2461 actggttttt aaaattattg agtaatttcc aggttcaact gctttcaggg tcgcaaaata
     2521 ttcttcggac tcacccgcat ctattttctt aaatacctgt cgtagatctc cctcttttac
     2581 ttccattcct ttcggaatcg ctccttctat aagtattgat tccgcagata cattgccatt
     2641 gtttgtgatt ttcactgtgg ctttgaccga ctcacctgtt aatatattat ttttatcaag
     2701 tgtaataacc gtgctaaggt tggatttttc ttttttttca tcaagaacgg ttattgaaag
     2761 ggaatttgac cttgaggtat ttgagccata ctttattgtc gtaggttcaa tatcatatgt
     2821 gcctgattcc actgccttca atgcaaacaa aagctccagg cgttctcccg cttttagttc
     2881 atcctgtaag gcgggaggaa aacttgaccc gggcgctctc gcaaatccct gcggcagctt
     2941 ttcatccaga gaaaggttgg tagctgtccc gttacctgtg ttttccagat tgatcttaaa
     3001 ttctatgaca tcccctttat taagtgttgt tttatcagcc gattttgtca atactatgtt
     3061 cggaattttc tcttcggcta cagatatttc aacattgtat tctattggct ttggccaatt
     3121 atcagggtat tcaatcgcga tctttgcagt tttttggata atagaggata aattgagcgt
     3181 gattgtatta aatagatcta aaggatcacc ttcagttaat gtatgattat ctgatgattt
     3241 tcctgaaatt tctttgattc taatataacc agagtattcg ttaatttcga aaacctccac
     3301 tttgtaattt aggttctcaa aatttaaacc ggtattattt tccagataaa catattgggt
     3361 aattttctcg gcattcacag gcatcgaaaa aaataataaa attaatatta gaaaagataa
     3421 ttctttcaat aaggtataat cattttttcg tttcggaatc atcaacaaag gagtctataa
     3481 tatcgataat atttaagcct tatgagtttg attttataat gaaaaaaata ttttacattt
     3541 cgatccggga tagtacccca tgaagtttgt gaggtggaag tttatagaat tgcacaaagg
     3601 caggcgtaag cctctttatc atggaatata tcctccagat aacattatct gaaacaatat
     3661 cttcaagacc atagaagatg gtcttttcct ctattccatt ctcctttaat agcgtctcga
     3721 catcaacgac ttccatatat cccatatata tctcaaatac ccttaaccca tccgcaaggt
     3781 cgctctttaa cgaaccggaa atgccgaagg gattctctgt ttttataagg gaaacaatta
     3841 tgttgtcctc ataaataatg ttattattga acatagtatt ggccatgtaa ggtgggatct
     3901 ttataatctc ctttgcaaag aaaagggctg tgccttttat tttgggcata ttattatata
     3961 cttgattata ttccttaaga aaatcatcaa gcaccattgg tgaaagggtt ttactaagtt
     4021 ttttctgccc ggatgtatat atcataatta cactgaatgg aattaaggca aggattattg
     4081 accagtaacc tccggaaggt atcttatgga gacttgagag gaggaaagcc atatcaacaa
     4141 atgtaactaa tgctgcaata ctggttttta atctattatc ctttaaataa aatatccatg
     4201 tcatcattat gcctgtcaag gtcatagtcc ctgtaacggc aagaccgtac gctgcagcaa
     4261 gactatgcga ttccttgaat aggatcatga ccataagcac agaaaaaagc aaaaaccaat
     4321 tcactgaatc tatgtatatc tgcgattgga gcttccttga tgtata
//
LOCUS       DATGBN010000041         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_158506, whole genome shotgun sequence.
ACCESSION   DATGBN010000041 DATGBN010000000
VERSION     DATGBN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158506"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /gene="speA"
                     /locus_tag="VKL21_00880"
     CDS             complement(<1..1731)
                     /gene="speA"
                     /locus_tag="VKL21_00880"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HMB44353.1"
                     /translation="MWKIEDAIELYGIERWGSGYFSFNRNGNLIIRPTKNDTQVIDLK
                     NVVDNLVSKKINFPILFRFPQILESQIKALNGAFSHSISEFRYNSSYQGIFPMKVNQR
                     KEVIEEIVKSGKKYNMGLEVGTKAELLAAMSFDLSSDALLICNGFKDDEYLHLALNAI
                     KLNNKVVIVIDEFSETKRLLEIAKQLSVKPIIGIRAKLYSKGSGKWAESGGESAKFGL
                     STTEILECIKIIREYNMLDQLQMLHFHIGSQITEIRRIQKAVKEAARVYAKIKIKHIN
                     IKYFNIGGGLGIDYDGSKTSSESSANYTMQEYANNVVYSLKEVCDEENVSHPIIISES
                     GRAISSYHSVLVINIKGNKNGGLNKPVELRGNEPHIITELYDAHKEINIKNYVEFYHD
                     ALLHREELLSLFNLGEIELEDKSMGEILFWQICGKAVGFAKETQNKSDDFDDLNKLLS
                     KKYIGNFSVFQSVPDFWAIKQLFPVVPVSRNNEKPTEYGTIVDITCDSDGEIDKFVDL
                     KDVKEILELHELNNGSYYLAFLLIGAYQDTIGDYHNLFGSANEAHIIVDETGGWHIKQ
                     IVNGDRNCDVL"
     gene            complement(1838..2317)
                     /locus_tag="VKL21_00885"
     CDS             complement(1838..2317)
                     /locus_tag="VKL21_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44354.1"
                     /translation="MNTGSTKDTKGTWIEYNAEVPGNLKSSLLKGGITTHGFSGTSAS
                     ILLTFANPGFPTHIASIDPAVQIYGHEFNTGQTQTGKNNKIGLFGNFKIVRIIGLMAL
                     ISYMLVILSTWIFANLQGYMYFSAGEPVLSIKYPEWILGFIGIFVAADYLHRELNSY"
     gene            2599..3123
                     /locus_tag="VKL21_00890"
     CDS             2599..3123
                     /locus_tag="VKL21_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44355.1"
                     /translation="MTPKLNIGKNSILISAILLIISVLLIIDRLLATGPVQLILADGK
                     AVPIESASFFSINEVLFFIITAWLGGMSFLYIILNSKEIPELKDKEIPESKREIAVIP
                     ESKSILAATLLEGDEKVLFQNIVDNDGILQRELIIKTGFSEPKVSRLLDKAERRGLII
                     RQRDGMGNRVFLTR"
     gene            complement(3428..3637)
                     /locus_tag="VKL21_00895"
     CDS             complement(3428..3637)
                     /locus_tag="VKL21_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44356.1"
                     /translation="MEDEKIKHTVNDLTVKIDNKINELSKSIEKTIGEKQEYTEGKIN
                     ENPLAYIAGAFLGGVIMGYMMGSRK"
     gene            complement(3664..4359)
                     /locus_tag="VKL21_00900"
     CDS             complement(3664..4359)
                     /locus_tag="VKL21_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44357.1"
                     /translation="MYLFNWDNIPGKDDGKLLEHLEQKYGVDWVRTAKIEKTDEYEIR
                     IFTEKNLLTLKLNNEKTKVTLTINNVITDEFIAKIENSKVNIYTDVEQEKQDIRRGVK
                     LESRYPYSMLGALVLAGLIIVFVMGYYGTQSAGIFSPREAFFVIGASILILTAASAYL
                     LFTLRNTVSVLKIHEEELQSMRDTMQERRISSLESTRSGATTSLGPQVAALEPQLASL
                     EKRVTNLEHVSSS"
BASE COUNT         1383 a          915 c          813 g         1540 t
ORIGIN      
        1 aaggacatcg cagttcctat cgccgttcac gatttgtttg atgtgccatc cgccagtttc
       61 atcaacaatg atatgcgctt catttgccga gccgaacaga ttatgatagt cgccaattgt
      121 atcctggtat gcaccaatta gcaggaaagc aaggtaatac gacccgttat tcagttcatg
      181 aagctcaaga atttctttta catccttcaa atccacgaat ttgtcgatct caccatcgga
      241 atcacatgtt atatcaacta tagtgccata ctccgtgggt ttttcgttgt tcctgctcac
      301 agggacgacc gggaataatt gttttatggc ccagaaatcg ggaactgact ggaaaaccga
      361 aaaattcccg atatatttct tggagagaag cttattaaga tcatcgaaat catcggattt
      421 gttctgcgtt tcctttgcaa accccacagc ttttccacat atctgccaga aaaggatctc
      481 tcccattgat ttgtcctcca attcaatttc accgagatta aaaagagaaa gaagttcttc
      541 acgatgaagc agggcatcat gataaaactc tacatagttt tttatattga tttctttatg
      601 tgcgtcgtat aattccgtta tgatatgagg ctcatttccg cgaagttcaa caggtttatt
      661 taaacctcca ttcttatttc ccttgatatt aataacaaga acagaatgat atgacgaaat
      721 cgctctgccg ctttcggata ttattatggg gtgagagaca ttttcttcat cgcatacttc
      781 tttgagtgaa taaaccacat tatttgcata ttcttgcatt gtgtaatttg cgctggattc
      841 ggatgaagtc ttgctgccgt cataatcaat tccaagacct ccaccgatat tgaaatattt
      901 gatgttgata tgctttattt ttattttagc ataaactctt gctgcttctt tgaccgcttt
      961 ctggattctc cttatttcag ttatctggga gccgatgtgg aaatgaagca tctggagctg
     1021 gtcaagcata ttgtattcac gtatgatctt tatgcattcg agtatctccg tagttgaaag
     1081 accaaatttt gcagattctc cgccggattc agcccattta ccgctgccct ttgagtagag
     1141 tttcgccctt atgccgataa tgggtttcac cgataattgt ttagcgatct caagcagtcg
     1201 ttttgtttcg ctgaactcat caattactat aacaacttta ttattcagtt tgatcgcatt
     1261 caatgcaaga tgaaggtatt catcatcttt gaatccgttg catatcaaga gggcatcgct
     1321 gcttagatcg aatgacattg cagccagaag ttctgctttg gtcccaactt ccagtcccat
     1381 attgtatttt ttcccggatt tgacaatttc ttctataact tcttttctct ggttcacttt
     1441 catgggaaaa atcccctgat atgaactatt atatctgaat tctgatattg aatgtgaaaa
     1501 agcgccattc agggccttga tctgggactc aagaatttgg ggaaacctga ataggatcgg
     1561 aaaattaatt tttttggata caagattatc aaccacattt ttgaggtcaa tgacctgggt
     1621 atcatttttt gttggcctga tgatcaaatt cccattcctg ttaaatgaaa aataaccgct
     1681 tccccagcgc tctattccat aaagttctat agcatcctca attttccaca tttattccga
     1741 attcacgtcc tcttttatga taaatccact gcaagaatac cttatcaggg ttaaagtttt
     1801 ccgtcgaata tcttataaca tataattatt aatataatca atatgaattt aattctctgt
     1861 gcaggtaatc ggctgcaaca aaaatcccga tgaatcccag tatccactca ggatacttta
     1921 tagacaatac aggttctcct gcggaaaaat acatatatcc ctgcaagtta gcaaatatcc
     1981 atgtagatag aattactaac atgtagctta tcagggccat gaggccaata atcctgacaa
     2041 ttttaaagtt accgaataaa ccaattttat tattttttcc tgtttgggtc tggccggtat
     2101 taaattcatg tccatatatt tggactgccg gatcgatgga agctatatgg gttggaaaac
     2161 caggatttgc aaaagtaagt agaattgatg cagaggttcc ggaaaaacca tgagttgtaa
     2221 tgccaccttt aaggagagaa ctcttaaggt ttcctggcac ctctgcatta tattcaatcc
     2281 atgtgccttt cgtatccttt gttgatcctg tgttcacacc attactttat ttccggatat
     2341 attaaagtta actcataata gtaatcatta tgatggctga aattaacaat ttactcgtgt
     2401 gaactaaaaa cggcaaataa aaagcatggg acatcgataa agctataaga aagtattttc
     2461 aattatagca tttgcttcca aaaaccgtta gaaaggttaa gaaggctaat ggtaaagcta
     2521 tgagtagttc aaaataagtg ttaaaaaaat aacccggcag tttttttagc atttagcggc
     2581 aaaatatact ttgaacaaat gacaccaaaa ttaaatatcg gcaaaaatag tattttaata
     2641 tcggccattt tattaattat ttccgttctt ctaataatag atagactcct ggcgacaggt
     2701 ccggtccaac ttattttagc ggacggtaaa gcggtgccga tagagagcgc ttcttttttt
     2761 tcaataaatg aagtattgtt ttttataata acagcctggc taggcggaat gtctttcctt
     2821 tatataatcc tgaactcaaa ggaaatacct gaattaaaag ataaagaaat acctgaatca
     2881 aaaagagaaa tcgctgttat cccggaaagt aaaagcatat tagcagccac tcttcttgaa
     2941 ggcgatgaga aagtattatt ccagaatata gtagataacg atggaatatt acagagggaa
     3001 ctcattataa aaacaggttt ttcagagcca aaggtaagca ggcttcttga taaggctgaa
     3061 cgaaggggat tgataattcg ccagagggat gggatgggaa acagggtatt tctaacaaga
     3121 tgataagatc aattataaat ataaattaat aggaatatgc ctgcatctgt atcgaattgt
     3181 tttaataatg cttgaaaaaa aaagaaaaat ttatagatac agaaactttg ttggcgatta
     3241 taacggccta cgaccttcga taactctgag cagtatcaag atgatagcaa tgaccaacaa
     3301 tatattgatt aaagccccaa ctgcaccgag cgaccccaat agccatagaa tcaccagaat
     3361 cacaattaat gtttctatta tatccattat ctaacctcct taacatcttt taccatactt
     3421 tgttgtttca ttttctacta cccatcatgt aacccatgat tacaccgccg aggaatgctc
     3481 ccgctatata agcaagagga ttttcattta tcttcccttc agtatattct tgtttctccc
     3541 caattgtctt ttcaattgat tttgatagct cgttaatttt gttatcaatt ttgacggtta
     3601 ggtcattaac tgtatgtttt attttttcat cttccataaa ttaacctcat agtcacatgt
     3661 tcattaagat gaactaacat gctccaggtt tgtaacccgc ttttccaggc tcgcgagttg
     3721 tggttccaat gcagcaactt gtggacctag agacgtggtt gcaccagatc gtgtactctc
     3781 aagtgaggag atacgcctct cctgcatagt atcacgcata gattgcagtt cctcttcgtg
     3841 gatcttgagg acgctgacag tattcctcaa tgtgaagagc aggtacgcag atgcggcagt
     3901 caggataagt atggaagcac cgatcacaaa gaacgcttca cgtggggaaa atataccagc
     3961 gctttgagtt ccatagtaac ccattacaaa tacaataatc aatccagcga gaaccagagc
     4021 gcctagcatt gaataagggt atctgctttc cagttttact ccccgcctga tgtcttgctt
     4081 ttcttgctca acatccgtat atatatttac cttactattt tcaatcttcg caatgaattc
     4141 atcggttata acattgttga tcgttaatgt taccttagtt ttttcattgt taagtttgag
     4201 tgtaagaagg ttcttttcgg taaatattct tatttcatat tcatcggttt tttcgatttt
     4261 tgctgttctt acccaatcga caccatactt ctgttctaga tgctctaaga gttttccatc
     4321 atcttttcca gggatattat cccaattaaa taaatacata ttttttttct ccttttatct
     4381 cttttattta atttccgact gagtagttat ccccactctc gcaattttca ggatttcccc
     4441 actctcacaa tcttaaggta cttctccgtt ctcacaatat tatggggttt tccgcccagt
     4501 ctttagaaaa aataaaaaat tttaggatta tacatatctc tatatttttg tcgtcattat
     4561 ctattttttc taaacagtaa atataatatt gttgtaacaa ccaatactat acctgatgta
     4621 aatccggggg cctttgttgg agaaggaaag a
//
LOCUS       DATGBN010000042         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_119956, whole genome shotgun sequence.
ACCESSION   DATGBN010000042 DATGBN010000000
VERSION     DATGBN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119956"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="VKL21_00905"
     CDS             complement(<1..1442)
                     /locus_tag="VKL21_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HMB44358.1"
                     /translation="MVSIKDMSLSKKLLGGFGIVSILLIIVAVVSITTLGTIESENNK
                     VIENTIMMKEKGLTMDVDMLSARRNEKDFFARHDLSYVDPVKTFVVNVAKDAQDIQAL
                     DVPQDDKDIAAKIVVNIEGYQKAFLETVELDKTKGLDETQGLQFEMRSAVQAVEADIT
                     KQNNDQLLANMLTLRRNEKDYIMRQDVAYQTKLHDNEKILKNNLAASNLPQDVKDGIN
                     AKLIVYTAAFDKIVDMDAKIASKTAEFTTYVHAIEPAIDTFEKAAEADQVAALAEIAS
                     TNATSKTTVIILSIIAVITGLGIGIYISRIITKPMDSMLNASNRVAAGDLTVEVRSDS
                     KDEVGQLATAIQTMTQSLKGVLGKVQMSAMNVASTAQELSASSEQMKASTDQISSTTQ
                     DIATGVSSQASKMSEISRAMKEMSESVQQVATNSQKASEGANSASTTAQRVGKMSEDV
                     AKKMTDIQSSVDNSATVIKQLDGKSQEIGE"
     gene            1743..2033
                     /locus_tag="VKL21_00910"
     CDS             1743..2033
                     /locus_tag="VKL21_00910"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HMB44359.1"
                     /translation="MKKKVLNFTVLIEQDEDGIYVAKVPDIIGCYTQGKTVKQAMKRI
                     KEAIQVCIEADDPGSFSFIWLEPFKKCRNFCSAAFFYKFWPIVQCGLLQPAL"
     gene            complement(1917..2282)
                     /locus_tag="VKL21_00915"
     CDS             complement(1917..2282)
                     /locus_tag="VKL21_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44360.1"
                     /translation="MGLKNHSEIGWVLREYNDLTSRALDMGVKSPDSHYADGKIKGKA
                     SRDKNVEGPKKPEKETSPARKMVFSVPLVEPDIQKEESGLERRLQKPTLNDRPKFVEK
                     GSATEIPAFLEGFKPNKRK"
     gene            complement(2369..3121)
                     /locus_tag="VKL21_00920"
     CDS             complement(2369..3121)
                     /locus_tag="VKL21_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44361.1"
                     /translation="MRIKSGIEGFDALVEGGFLQGREYLLSGSPGSGKTTFGVQFLTA
                     GALEGEAGGYIALSESIGTIVEDMSRYNPQIGDLIKNKKLFFIDLGPSVNYGEYDEVS
                     NIITPDFNQQSGDSPANKPPTPFTVFKEVENQVKQFGIKRLVIDSISSIRFTSQSPAS
                     EERSVGRFIRNLKNLGCTTVLLSELTKPDAYTIEQFASHGVIFLHNFMNKDGTMVRAL
                     QIIKMRGTKHDCDMRGIEFSDKGLRVGKPLKK"
BASE COUNT          786 a          701 c          622 g         1056 t
ORIGIN      
        1 atttcgccga tctcctgcga tttgccgtca agctgtttta tcacagtcgc tgaattatcc
       61 acacttgatt ggatatccgt catctttttc gccacatcct ctgacatctt acccaccctc
      121 tgggctgtgg tactggctga atttgcacct tcagaggctt tctgggagtt cgttgcgacc
      181 tgctggacgc tttctgacat ttccttcatg gcgcggctga tctcagacat cttggaggcc
      241 tgtgagctta cgcctgttgc tatgtcctga gtggtgcttg agatctggtc agttgaggcc
      301 ttcatctgct cacttgaggc agagagttcc tgggcagtgg atgccacatt cattgctgac
      361 atctgcacct ttccaaggac gccttttaag ctttgggtca tggtctggat agcagtggcg
      421 agctgtccta cttcatcttt agaatcactc ctgacctcta ctgtgaggtc gcctgctgcg
      481 actctattag atgcgttgag catgctgtcc attggttttg tgatgatacg ggagatatat
      541 atcccgattc caagacctgt tattacagct atgatagaaa gtattatgac cgtggtttta
      601 gaagttgcat tcgtgctggc tatttcggca agcgctgcca cctggtctgc ttcagcagct
      661 ttttcgaagg tgtctattgc aggttctatc gcatggacat aagttgtgaa ttcagctgtt
      721 tttgatgcaa tcttagcatc catatccacg atcttatcaa aagctgccgt atatacaatg
      781 agcttggcat tgatgccatc tttaacatct tggggcaagt tgctggccgc cagattattt
      841 tttagtatct tttcattatc atgtaatttt gtttgatacg cgacatcctg tctcattatg
      901 taatcttttt catttcttct taatgtcagc atatttgcta aaagctggtc attattttgt
      961 tttgtgatgt ctgcttcaac agcttgcact gcactcctca tttcaaattg gagtccctgg
     1021 gtctcatcaa ggccttttgt cttatcaagt tcaacggtct caaggaacgc tttttggtaa
     1081 ccttcgatat tcactactat ttttgctgct atgtccttat cgtcctgtgg gacgtcaagt
     1141 gcctgtatat cctgtgcatc ttttgccaca tttacaacaa aagtttttac tggatcgaca
     1201 tatgataaat catgacgtgc aaaaaagtct ttctcattac gtcttgctga aagcatatct
     1261 acatccatag ttaggccttt ttctttcatc atgatcgtat tttcaatcac tttattattt
     1321 tccgattcga tagtaccaag tgtggttatg ctgaccacag cgactattat aagcaggatg
     1381 ctgacaatgc caaatccgcc aagcagcttc ttgcttaaag acatatcttt tatgcttacc
     1441 atttatgtta ttcctccatt tattggggtt caacctgaaa gatagccctt cagatgattc
     1501 ttaaacaaca gagagaaatc cacctgttta taaacggatt tgtgaaaaag gtgtgaaatg
     1561 aaatgatttg gtctgaatct atagcagtcg cctttgctct tgatatgcta acataattga
     1621 tatatcatag ctatcaaaat gatagaaaaa gctatcaata tgatattaaa ttgctaaagt
     1681 tctcataata atagttattt tataaattta tgatggaaaa agcgatcaat ccgcgcgtta
     1741 aaatgaaaaa aaaagtttta aacttcactg tccttatcga gcaggacgaa gatggaattt
     1801 atgtagctaa ggtgcctgac attatcggct gttatactca gggcaaaaca gtaaagcagg
     1861 caatgaagcg cataaaagaa gctatacagg tatgcattga agcagatgat cccggatcat
     1921 tttcttttat ttggcttgaa cccttcaaga aatgccggaa tttctgtagc gctgcctttt
     1981 tctacaaatt ttggccgatc gttcaatgtg ggcttttgca gccggcgctc taaacccgat
     2041 tcctcttttt gaatatcagg ttcaaccaga ggaacagaaa aaaccatctt cctggccgga
     2101 gatgtttcct tttcaggttt ttttggtccc tcaacatttt tatcccggct ggcttttcct
     2161 tttatcttac cgtcagcata atgagaatca ggtgatttga cgcccatatc cagagctctg
     2221 gatgtcaggt cattatactc tctcagtacc cagcctattt cagagtggtt cttgagtccc
     2281 acttcaaagc caagctcata tattttcttt ataagttctg cccggtcctt ttcttcaatt
     2341 ttactggtaa tacttttaac cccctggttt attttttgag cggttttcca actcttaaac
     2401 ctttatccga gaattcaatg cctcgcatat cgcagtcatg ctttgttccg cgcattttaa
     2461 tgatctgcaa agcccggacc atagttccat ccttattcat gaaattatga aggaatatca
     2521 cgccatgcga tgcgaattgt tctattgtat aagcatcggg cttggtaagt tcggaaagca
     2581 gtacggtcgt gcatcccagg tttttaaggt tgcgtatgaa cctgcccacg gaacgctctt
     2641 cagacgccgg gctctgggag gtgaatctga tcgaagagat cgagtcaatc accaatcttt
     2701 tgataccgaa ctgttttacc tggttttcca cttctttaaa tactgtaaaa ggagtgggag
     2761 gtttattcgc aggcgagtcg cctgattgct gattaaaatc aggagtgata atatttgaga
     2821 cctcatcata ttccccgtaa tttacagatg gaccaagatc tataaagaat aattttttat
     2881 tctttatcag atcaccgatc tgcggattat atcttgacat atcttctaca atagtcccga
     2941 tactttcgga taatgcgatg tatcctcccg cttcgccttc cagcgcccct gcggtaagaa
     3001 attgtacgcc aaaagtagtc ttgccgctgc ccggggaacc gctcaacagg tactcccttc
     3061 cctgcagaaa tcctccttca accagcgcat caaacccttc aattcctgat tttattctca
     3121 taataacctc ttaacatgta agtataggtg tttgattttt tttct
//
LOCUS       DATGBN010000043         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_3157, whole genome shotgun sequence.
ACCESSION   DATGBN010000043 DATGBN010000000
VERSION     DATGBN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3157"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..929
                     /locus_tag="VKL21_00925"
     CDS             108..929
                     /locus_tag="VKL21_00925"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HMB44362.1"
                     /translation="MKFKSTAESMGHKFEFIYKDDISKIPDYDALFIRATTDPSSSAY
                     IFSRLAEQSGLKVIDDPHSIRTCSNKAILHDLFHKNNIPSPKSILFHGNYSRETLDTI
                     FMTLGLPVIIKTPYTRFSSHVEKASNETQFIEISKHLLRKTKVLVLQEYIQSDFDWRV
                     GILSNEVLYLCKYCIPDGGWKVRSKINGKNVWGDTIALPRDSISPGLKELSINLSKCI
                     GNGLYGLDVKEINGVYKVIEINDNPSIYDGYEDTVDRDIYEKIVNYLTLDSSTTI"
     gene            complement(961..1455)
                     /locus_tag="VKL21_00930"
     CDS             complement(961..1455)
                     /locus_tag="VKL21_00930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HMB44363.1"
                     /translation="MRKGARDGSLKWQIRWATKEDLDSLFGLENLCFKEETFHRKQLK
                     YLLLKARSIVLVAEIGGNIIGSIIILLRERILNARIYSLNVHPEHRRRGIAGSLMDTA
                     QDILEEKGYDNVTLEVGINNRIAQNLYRSKDFVVDKELSCYYKNGDDALHLIRKLERK
                     LNAA"
     gene            complement(1425..>1847)
                     /locus_tag="VKL21_00935"
     CDS             complement(1425..>1847)
                     /locus_tag="VKL21_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HMB44364.1"
                     /translation="KRGSFMKYNNIQISADGNAEDIAPFALAIHELLGLPVTMRSLNK
                     KGVRIEKGNILDTNYSGPVLESVLKENKTIRCIPTSGKYAGIPVIVVPIRNKEGYGIA
                     AIGVVDVVGTVDLGFIFGDYPEVVKQVQECVRARVTVP"
BASE COUNT          533 a          397 c          350 g          567 t
ORIGIN      
        1 cgttcatgat aaagtgcgcg agataaaaca gggcatttaa tcctatgaaa atagcctgct
       61 ttgtggaagc atacaatttt attattaaat ccgagtgcag cgctcttatg aaattcaagt
      121 ccaccgctga atcgatgggg cataagtttg aatttattta taaagatgat atttcgaaaa
      181 tcccggatta cgatgccctt ttcatacggg cgaccactga tccgagcagc tcggcgtaca
      241 ttttttcaag acttgccgaa caatcaggat tgaaagtcat tgatgaccct cactcaataa
      301 ggacatgttc caataaggcg atactgcatg atcttttcca taagaataat ataccttcgc
      361 caaagtcaat tttatttcac gggaattatt ccagggaaac tcttgatacg atatttatga
      421 ctcttggatt acctgtaata attaagacac catatacacg attttcgtcc catgtcgaaa
      481 aagccagcaa tgagacgcaa ttcattgaaa tatcgaaaca tttgctcagg aagaccaaag
      541 ttctggttct ccaggaatac attcaatcgg attttgactg gcgtgtaggg atacttagca
      601 atgaagtgct ttatttatgc aaatattgta ttccggatgg gggatggaaa gtaagatcga
      661 agataaatgg gaaaaatgta tggggagaca ctattgcatt accaagggat tcaatttctc
      721 ccggattgaa agagctctcc attaacctgt caaaatgtat aggcaatggt ctttatggtc
      781 ttgatgtgaa agaaataaat ggcgtataca aggtcatcga gataaatgat aatccaagta
      841 tatatgacgg ctatgaggat actgtagata gggatattta tgagaagatc gtgaactatc
      901 taacactcga ttcatccacc accatttaag ttgtgccatt tggccagtac tcctgggtct
      961 ctatgctgca ttgagtttcc gttctaattt ccttataaga tgcagcgcat catcaccgtt
     1021 cttatagtaa caggatagtt ctttatcaac aacaaaatcc tttgacctgt atagattctg
     1081 cgcgatcctg ttattaattc ccacttccag cgtgacatta tcatatcctt tttcttccaa
     1141 tatatcctgg gcagtatcca tgagcgaacc tgcgatcccc cttctcctgt gttcgggatg
     1201 tacattcaaa gaatatatcc ttgcatttaa tattcgttct cttaacagga ttattattga
     1261 cccgataata ttgccgccta tttcggcgac caatactatc gatctggctt ttagcaacag
     1321 gtattttagc tgctttctat gaaatgtttc ttccttgaaa cataaatttt caagaccaaa
     1381 aagtgaatca agatcctctt ttgttgccca acggatctgc catttcaagg aaccgtcacg
     1441 cgcgccctta cgcattcctg tacctgtttt accacttcag gataatctcc aaagatgaaa
     1501 ccaaggtcta ccgtacccac cacatcaact accccgattg cagcaatgcc atatccttct
     1561 ttatttctta tgggtacgac gataacggga attccggcat atttgccgct tgtgggaata
     1621 caacggatcg ttttgttctc tttcaggacg gactcaagca cgggaccgga ataattggta
     1681 tcaaggatat ttcctttctc gatcctgact ccctttttat tcaggcttct catagtaacc
     1741 gggagcccaa gaagttcatg tatcgccagc gcaaacggag cgatatcttc ggcgtttcca
     1801 tccgctgata tctgaatatt attgtatttc ataaaactgc ctctctt
//
LOCUS       DATGBN010000044         5114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_236404, whole genome shotgun sequence.
ACCESSION   DATGBN010000044 DATGBN010000000
VERSION     DATGBN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236404"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..651
                     /locus_tag="VKL21_00940"
     CDS             265..651
                     /locus_tag="VKL21_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44365.1"
                     /translation="MLALKIPVLIITILFALIGVYLAIMIYLAGRKIDPATLRARAFL
                     NESFLKESWELILMSLILFIIRAVVELEEVLEEVIDAKSSEILDEIIVLGILICLILL
                     LYKWLKLMNPPKPNLKDKSRGSLSDK"
     gene            729..1133
                     /locus_tag="VKL21_00945"
     CDS             729..1133
                     /locus_tag="VKL21_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HMB44366.1"
                     /translation="MKTVYLAAPLFGEAECDFNRKLRNELISAGFKVFLPQEDSNNIK
                     DNMDRQKIIFNKNLKGIENSDILVAVIDGADVDSGTAWEIGFAFSKGKPVLGLRTDFR
                     TLGIEGTVNLMIERSVILCMSVSELLNHLKSI"
     gene            1139..1699
                     /locus_tag="VKL21_00950"
     CDS             1139..1699
                     /locus_tag="VKL21_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMB44367.1"
                     /translation="MKITSISGSPNAGGNNEKMADIVLEIAKERGYETDRIFLSNLRV
                     EPCTDCGACKISRDCPIDDDMKDIYKKLETSDAFIVSSPVFFGSMSAQLKALLDRTIL
                     LRRNGFLLKGKFGAAMAIGGSRNGGQEFTIQTIHSWMHVHGMVIVGDAGHFGGIALKP
                     FESDKEGLKTVKDTANMLCDTLSRLK"
     gene            1700..2107
                     /locus_tag="VKL21_00955"
     CDS             1700..2107
                     /locus_tag="VKL21_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44368.1"
                     /translation="MDEYHLIKQFSKPQEGEYVPVTFIEFERKLVGWSTELKRSVYIG
                     SEEEKAKLKRVREVNLMIAINHLSGKLSSIELSDQEKEQFEEVYNLFLEKGGQLFYTR
                     KKTGAKNIPFFELREPEKKVRDKPEKNLLSDML"
     gene            2144..3154
                     /gene="queA"
                     /locus_tag="VKL21_00960"
     CDS             2144..3154
                     /gene="queA"
                     /locus_tag="VKL21_00960"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HMB44369.1"
                     /translation="MKLSEFDYHLPKELIAQSPVEPRDASKLMVVGQKIEHGFFSNIL
                     DHFEKGDTLVLNDSRVIPAKLTGKKSTGGHVEALVVSRNDAGHVCLIRGKNIREGSIL
                     IFGELEATVLQLLERPNTNRYLVNFNCNGNLPDILEKIGEAPLPPYIKTSLDNKNRYQ
                     TVYSKERGSIAAPTAGLHFTKELLERIKAKGVNIAYVTLHVSLGTFTPVKALDIETHV
                     MEAEYISVRKENADIINATQGKVIAAGTTTAKALESSCIEGKIVEKEGFRQLFIYPPY
                     RFKSRIDAIITNFHLPKSTLLMLVSAFAGRQRLMEAYDEAISHSYRFYSFGDAMLVFR
                     GD"
     gene            3161..4261
                     /gene="tgt"
                     /locus_tag="VKL21_00965"
     CDS             3161..4261
                     /gene="tgt"
                     /locus_tag="VKL21_00965"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMB44370.1"
                     /translation="MFNLIHKDKNTGARAGKLSTNHGIIETPAFMPVATKATVKTITP
                     DELYEMGTEVLISNAFHLLLSPGMEVLQSSGGLHKFMNWQGGVFTDSGGFQMIRKDFP
                     FKINDEGIIYKNLRDGKKYSYTPQICLENQKILGSDVAMILDECPAYGSEFGVVKTSM
                     ERTIKWAKMAKDVEQNHGQLFFAILQGGTFAELRKKCAEELIEIDFDGYGIGGLSIGE
                     PKEIMNDVLKLSVPLVPEDKARYLMGVGSPIEILNAIESGIDIFDSAFPTRNARHQTL
                     MTSKGSIDIARNKFTADFSPIDENCGCYTCKNYTRAYQHHLFKESELLALRLASIHNL
                     HFIQSIVKGAREAILEDSFLEYRKNVIAGLLP"
     gene            4258..4728
                     /gene="ruvC"
                     /locus_tag="VKL21_00970"
     CDS             4258..4728
                     /gene="ruvC"
                     /locus_tag="VKL21_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMB44371.1"
                     /translation="MIVIGIDPGLATVGFGVIKTEKEKVTPISYGCVRTSPDKQTPQR
                     LLEIYNEITSLFEKYKPEVIAVEKLFFSKNVTNALSVSEARGVIFLAAQQQNIPILEY
                     TPNQVKQAITGSGRADKKQVQEMIKRLLGLDEIPEPDDAADGLSIVLCHINIMR"
     gene            4732..>5114
                     /gene="ruvA"
                     /locus_tag="VKL21_00975"
     CDS             4732..>5114
                     /gene="ruvA"
                     /locus_tag="VKL21_00975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMB44372.1"
                     /translation="MISHICGNITHSGEGFVVIDIGGMGYQVFVTKPTLNELADKKEK
                     VKLHTYLHVREDALILYGFLSQSELEMFKLLISVTRVGPQIAMNILSQIRIEELAAAI
                     IHEDEKVLTRISGVGAKNAKRLILE"
BASE COUNT         1623 a          980 c         1179 g         1332 t
ORIGIN      
        1 tacgatttat tgtttctcat atctatccag aattaaagta aatatacaga tatactattt
       61 aatcttttcc acataataaa tatttttgat gagtgtaata atcattataa tggtggtatg
      121 atacaagagg ggaaataagg ataataaagc acatcaagag acacacatac catagaacgg
      181 aattttcata gtcaaatttc acatgaattt cacacttcca tatatatata tatacagtcc
      241 actgtatgtt tacatagaaa gattatgctt gcattaaaaa ttcctgtgct gataatcaca
      301 atattattcg cactcattgg tgtttacctt gcgatcatga tatatctcgc agggagaaaa
      361 atcgatcctg ctaccctcag ggctcgtgct tttcttaatg agtcattcct caaggaaagc
      421 tgggaactga tccttatgtc cctgatcctt ttcattatcc gcgctgtagt ggaactggaa
      481 gaagtattgg aagaagtaat tgatgcaaaa agctctgaga tccttgatga gatcatcgtt
      541 ctcgggatac ttatatgcct catcttatta ttatataagt ggctcaaatt aatgaatccg
      601 ccaaaaccga atctgaaaga caaatcacga ggttcacttt cagacaaata attgaaccat
      661 ttaaataatc gggaatactt tcacattgtt ctggaaatct tatttaacta acaggtgatg
      721 tataatatat gaaaacagtt tatcttgccg caccgctttt tggagaagca gagtgtgatt
      781 ttaaccgcaa gctcaggaat gagttaataa gcgccggctt taaggtattc ctgccccagg
      841 aagattccaa caatataaag gacaatatgg atcggcagaa gataattttc aataagaacc
      901 ttaaggggat cgagaactcg gatatactcg ttgctgttat agatggggcg gatgttgatt
      961 caggaaccgc atgggagatt ggtttcgcat tctcaaaagg caaacctgtc cttgggcttc
     1021 ggacagattt tcgtacactc ggtatagagg gcacggtgaa tctcatgata gagcggtctg
     1081 tgatcctttg catgagcgtt tcggagttat taaatcattt gaaatcaata tagcggacat
     1141 gaaaataaca agtatatccg gaagcccgaa tgccggaggc aataatgaga agatggcgga
     1201 tatcgttctc gaaattgcaa aggaacgggg atatgaaaca gacaggatct ttttatcaaa
     1261 tcttagagtg gaaccgtgta cggattgcgg cgcctgtaag atatccaggg actgcccgat
     1321 cgatgacgac atgaaggata tttataaaaa acttgaaacc agcgatgctt ttattgtaag
     1381 ttcgcctgtg tttttcggga gtatgtcagc ccagttaaaa gcccttcttg accgtacgat
     1441 ccttctcagg aggaacgggt tcctgctaaa agggaaattc ggtgctgcta tggctatagg
     1501 cggaagcagg aacggcggac aggaattcac gattcagacc atacattcat ggatgcatgt
     1561 acacggaatg gtaattgtgg gagacgccgg tcattttggc gggatcgctc tgaaaccgtt
     1621 cgaatctgat aaagaggggt taaagacggt aaaggatacc gctaacatgt tatgcgatac
     1681 tctttcgaga ttgaaataaa tggacgaata tcatttaata aagcaatttt ccaaacccca
     1741 ggaaggggaa tatgtgccgg taactttcat tgagttcgaa cgaaaactgg taggctggtc
     1801 aactgaactt aaaaggagcg tctatatcgg gagcgaagag gaaaaagcaa agctaaagag
     1861 agtaagagag gtaaatttaa tgattgctat aaatcattta tcgggaaaac tgtcctctat
     1921 tgaactctct gaccaggaaa aggaacaatt tgaagaagta tataacctgt ttctcgaaaa
     1981 gggcggacag ttattttata cgcggaaaaa aacaggagca aaaaacattc cattttttga
     2041 gcttcgcgaa cctgagaaaa aagtaaggga taaacctgag aaaaatctgc tgtctgatat
     2101 gctatgagag aaaatactta aacatcaatg cagactaagc ccaatgaaac tttccgaatt
     2161 tgactatcat ctgccaaaag aacttatcgc gcaatctcct gttgaaccaa gggacgcatc
     2221 aaaattgatg gtagtgggac aaaaaatcga gcatgggttt ttttcaaata tacttgatca
     2281 ttttgaaaaa ggagatactc ttgttcttaa cgattcaaga gtaatacctg cgaagctcac
     2341 aggaaaaaaa agcacgggag gtcatgtgga agccctggtg gtttcaagaa atgatgccgg
     2401 ccatgtttgc ctgatacggg ggaaaaatat ccgcgaaggt tcaatactta tttttggtga
     2461 gctggaagca acagtgcttc aacttttaga aagaccgaat acgaacaggt atcttgtaaa
     2521 ttttaattgc aatggcaacc ttcctgacat ccttgaaaaa ataggagaag cgccgcttcc
     2581 tccctatatc aaaaccagtc ttgataataa gaaccgttac cagacggtct attccaaaga
     2641 aagaggctca atcgcagcgc ccactgcggg ccttcatttc acgaaggaat tattggagag
     2701 gatcaaagca aaaggtgtca acatcgctta tgtcacgctt catgtaagcc tgggaacctt
     2761 tacacctgtt aaagcgctgg acattgaaac acatgtcatg gaagccgaat atatttccgt
     2821 acgaaaggaa aatgcagaca ttatcaatgc cacgcagggt aaggtcatag ctgcgggaac
     2881 aacaactgcc aaggcgcttg agagttcatg catcgaagga aaaattgtgg aaaaggaagg
     2941 attcagacag cttttcatct accccccata tcgattcaaa tcaaggatag acgctattat
     3001 aacaaatttc catctcccca aatccacgct tttgatgctt gtgagcgctt ttgcaggcag
     3061 gcaaaggctg atggaggctt atgatgaagc catatcacat tcatacaggt tttacagttt
     3121 cggggatgca atgcttgttt tcagggggga ttgagataat atgttcaatt tgatccacaa
     3181 ggataagaat accggagcaa gagccggaaa actcagtaca aatcacggca ttatagaaac
     3241 tcctgcgttc atgcccgttg ctacaaaagc cactgtaaag accataacgc cggatgaact
     3301 ctacgaaatg ggaacagaag tgttgatcag caatgctttt cacctgctgc tctctcccgg
     3361 tatggaggtt ctccaaagct ccggaggcct gcataaattc atgaactggc agggaggcgt
     3421 attcactgac agcggagggt tccagatgat acggaaggat tttcctttca agatcaatga
     3481 tgaagggatc atttataaga acctgcggga cgggaaaaaa tactcttaca ctccgcaaat
     3541 ctgccttgag aaccagaaga tacttggcag cgatgtggcc atgatacttg atgagtgccc
     3601 tgcttacggg agtgagttcg gggtcgttaa aacttcaatg gaacggacta ttaaatgggc
     3661 aaaaatggct aaagatgtgg aacaaaatca cggccagctc ttttttgcca tacttcaggg
     3721 cgggactttt gcagaacttc gaaaaaagtg tgcggaagaa ctgatagaga tagattttga
     3781 cggctacggg ataggcggac tatcaatagg tgagccaaaa gaaattatga acgatgtttt
     3841 aaagctgagc gtgcctcttg ttcctgaaga taaggcccgt tatcttatgg gtgtgggttc
     3901 acccatcgag atattaaatg cgatcgaatc gggcatcgat atttttgaca gcgcttttcc
     3961 cacacgaaat gcaaggcacc agacccttat gacctcaaaa gggagtattg atattgcccg
     4021 gaataaattt acagcggatt tttcaccgat cgatgaaaat tgcggctgct atacatgtaa
     4081 gaactataca agggcttatc agcaccatct tttcaaggaa tcggaactgc tggcacttcg
     4141 gcttgcctcc atccataatc ttcattttat ccagtcgatt gtgaaagggg caagggaagc
     4201 catacttgag gatagttttc tagagtatcg aaagaatgtg atcgcaggtc tattgccatg
     4261 atcgttattg gaattgaccc ggggcttgca actgtgggat tcggggtgat aaagacagaa
     4321 aaagaaaaag ttacgcccat aagctatggg tgtgtcagga catcccccga taaacagacc
     4381 ccacagcgcc ttttagagat atacaacgaa ataacatccc tttttgaaaa atacaaacct
     4441 gaggttatcg cagttgaaaa actctttttc agtaagaatg tgactaacgc tttgagcgta
     4501 agcgaggcaa gaggcgtcat atttctggca gcgcagcagc agaatatccc gattttggaa
     4561 tataccccca accaggtgaa acaggccata acgggttcgg gaagagctga caagaaacag
     4621 gtgcaggaga tgatcaagag gctccttggc cttgatgaga taccggagcc ggatgatgct
     4681 gcagatggac tgtcgattgt tttatgtcat ataaatataa tgagataata tatgatttca
     4741 catatctgcg gcaatatcac ccatagcggt gaaggatttg ttgtaatcga cattggcggc
     4801 atgggatatc aggtgtttgt gacaaaacca acattgaatg agctcgccga taagaaagaa
     4861 aaagtaaaac ttcatactta cctccatgtc agggaagatg cgctcattct ttatggtttt
     4921 ttaagccaga gcgaacttga gatgttcaag ctcctgataa gtgttacaag ggtcggcccc
     4981 cagatagcca tgaatattct ttcccagata aggatcgaag aactcgccgc tgcaattatc
     5041 catgaggatg aaaaagtcct gacacgcatc tcaggggtgg gggcaaagaa tgccaaacga
     5101 ctgatactgg aatt
//
LOCUS       DATGBN010000045         1690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_508351, whole genome shotgun sequence.
ACCESSION   DATGBN010000045 DATGBN010000000
VERSION     DATGBN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508351"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="hemB"
                     /locus_tag="VKL21_00980"
     CDS             <1..617
                     /gene="hemB"
                     /locus_tag="VKL21_00980"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HMB44373.1"
                     /translation="EYTSHGHCGMISEKHEILNDPTLEILGKTAVSHARAGADIVAPS
                     GMMDGMVGAIRRALDDNDFKDVPIMSYAAKYCSVFYGPFREAASSGFSFGDRASYQMD
                     PGNSDEALREVALDIEEGADIIMVKPAMTYLDIIYRVKKKFKVPTAAYHVSGEYSMIK
                     AAAQNGWIDEKRAIYESLISIKRAGADMILTYFAKDVALDLNSK"
     gene            617..1051
                     /locus_tag="VKL21_00985"
     CDS             617..1051
                     /locus_tag="VKL21_00985"
                     /inference="COORDINATES: protein motif:HMM:NF021503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2227 family putative metal-binding protein"
                     /protein_id="HMB44374.1"
                     /translation="MPGYNRHRLFNYIIFIAIVFFFYSENTTHPDIGRLLAICLSFYI
                     GTEFVTPDLDTNNAAYKRWGDMKILLLPYKWTFKHRQSSHNIFYGAIVRILYITIIIL
                     VFYYLIFKSLSTGLDISPVYGIIFLFGIIIANALHVLLDNIF"
     gene            complement(1097..>1690)
                     /locus_tag="VKL21_00990"
     CDS             complement(1097..>1690)
                     /locus_tag="VKL21_00990"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMB44375.1"
                     /translation="SEVYGDPLVNPQSEEYWGNVNPIGPRGVYDEAKRFAEAITMAYH
                     RYHGVETRIVRIFNTYGPRMRLKDGRVVPNFIGQALNGEDLTVYGDGSQTRSFCYVSD
                     EIEGIYRLMMSDYSDPINVGNPEERTILEFANLVLKLTGTSSKIIFKELPEDDPKQRK
                     PDIAKARKILNWEPKVSLEQGLEKTIQYFKSVLESEK"
BASE COUNT          462 a          360 c          352 g          516 t
ORIGIN      
        1 gcgaatacac ttcccatggg cactgcggta tgataagcga gaaacatgag attctcaatg
       61 accccacgct tgagatactg ggaaagaccg cagtgagcca tgcccgcgca ggcgctgata
      121 tcgttgcgcc ttcgggaatg atggacggta tggtgggagc gattcgcaga gcgcttgatg
      181 ataatgattt caaggatgtt ccaataatgt cctatgcggc aaaatactgt tcagtgttct
      241 acggcccatt ccgtgaagcg gcaagctccg gattttcttt tggcgacagg gcttcatacc
      301 agatggaccc gggaaattca gatgaggcgc ttcgtgaagt tgcacttgat atcgaagaag
      361 gcgccgatat cattatggtc aagcctgcaa tgacatatct tgacattata taccgggtaa
      421 agaaaaaatt caaggtgcct actgcggcat accatgtcag cggcgagtat tccatgataa
      481 aagcggcagc tcaaaatggc tggatcgatg aaaagagggc aatatacgaa tcgctgatat
      541 cgataaagcg cgcaggcgca gatatgatat tgacatattt tgcgaaagat gtagctcttg
      601 atctgaattc aaaataatgc cgggttataa cagacacagg ttatttaatt acattatttt
      661 tattgcaatt gtcttcttct tttattctga aaatacgact catccggata tcggacgttt
      721 actcgcgata tgcctgagtt tttatatcgg aacagaattt gtaactcctg accttgatac
      781 aaataacgct gcttataaac gctggggcga tatgaaaatc cttttgcttc cttataaatg
      841 gacattcaag cacaggcaga gttcacataa tattttttac ggcgcaattg tcaggatatt
      901 gtatataacc ataattatcc tggtatttta ttatctcata ttcaaatcgc tatcaaccgg
      961 gttggatata tcgccagttt atggcatcat ttttttattt ggaataataa tagctaatgc
     1021 acttcatgtt ttactggata acatttttta atattgatcg agattgttat ccttgtgggg
     1081 atgctcagaa gggagttcac ttctctgatt ccaggacact cttgaaatat tgtatcgtct
     1141 tttctaatcc ctgttcaaga gagactttcg gctcccaatt aaggatcttc ctggcttttg
     1201 cgatatcagg ttttctttgt ttcggatcat cttcgggcaa ttccttgaaa atgatcttcg
     1261 aactggtacc tgtcaacttt agtaccaaat ttgcaaactc aagaatagta cgctcttccg
     1321 ggttcccgac atttatcgga tcagaataat cagacatcat caggcggtat attccttcta
     1381 tttcatcgct cacataacaa aagcttctgg tctgggatcc gtctccatat acggtaagat
     1441 cctctccatt tagagcctgt ccaataaagt ttggtaccac acgaccgtct tttaagcgca
     1501 tgcgtgggcc gtatgtattg aaaattctca ctatccttgt ttcaacgcca tgatatctgt
     1561 gatatgccat agtaatggct tcagcaaacc tttttgcttc atcatataca ccccgtggtc
     1621 ctatcgggtt cacgttcccc caatattcct cactttgagg attcacaaga ggatcaccat
     1681 aaacctcgga
//
LOCUS       DATGBN010000046         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_430454, whole genome shotgun sequence.
ACCESSION   DATGBN010000046 DATGBN010000000
VERSION     DATGBN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430454"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1529
                     /gene="folP"
                     /locus_tag="VKL21_00995"
     CDS             303..1529
                     /gene="folP"
                     /locus_tag="VKL21_00995"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMB44376.1"
                     /translation="MIEKTIAGLKVGDDQPVRIMGVINLSKESFYKNSVVSPENVRDT
                     AINMINEGADIIDIGARSTWPLAGKISKDEECSRLVPAMKPLAGLPVPISVDTMFSDI
                     AKEALAAGANIINDVSGFTADDKMMGVAAKFACPVILMASNNIPGDPVGMDAIMESLE
                     RIIAYAQNNGISPDSIIIDPAIGKWTPRKLAIYDYETIDNIERLRIFKKPILAAISRK
                     SFMGDTLNKPATDRLYGSIAATAIAVRNGAHIIRTHDVAPTVDAVRVAEASRARIPIV
                     RSGTIEAELLEIKNIKDCQKAMLLIGVTSKGSQIMKKKTLIYNILINNISTTEALIIK
                     QEMLARGGDAALPRDAVSHETQKVSLIISGTQLQVERLINKIRQQVRDLPSIADMLTE
                     LMNQNNDTVFRYSRSK"
     gene            1526..2149
                     /locus_tag="VKL21_01000"
     CDS             1526..2149
                     /locus_tag="VKL21_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HMB44377.1"
                     /translation="MIISRQKEFIDILQLLAEKNIFIIGCGKCAKKQRVGGEPEVKDM
                     KKRLEDAGKNITGWTVLSSACSISSWMEVLSMNPEIDKSDALLVMSCGGGVSVISGFA
                     GIEVYPALDTESLGGVVCNEVVTEQCGMCGDCTIWLYGGICPVIQCAKGLLNGPCGGA
                     LEGKCEIEERLCAWDKIFERLKELKKLENLEKINEPKDHMRKVRVKT"
BASE COUNT          741 a          416 c          531 g          563 t
ORIGIN      
        1 ctgaaccaag agggcatttg acggttgaag gtgaactaac tattcgtgat atcaccaaca
       61 ctgtatccat tgatgcagtt tttagcggca tcgttgatga ccccctgggg caatacgcga
      121 attgctttca atggtaatac aaagatcagt cgtaaggatt ttggtctaat gaccgaatta
      181 atgcgggaaa ccggtgggtt gttggttgga aaggacataa ttattaatat ttcagctgaa
      241 ctcctatcac aaacctgata aaaggtacat ttttataact gtacaaagta ttaggttaaa
      301 acgtgataga aaaaaccatt gcaggattga aagtagggga cgatcagcct gtaaggataa
      361 tgggagtaat aaatctcagc aaagagtctt tttataaaaa ttcggtggtt tcaccggaaa
      421 atgtacgtga tactgccatt aacatgataa atgaaggcgc tgatattatc gatattggcg
      481 ctcgttcaac ctggcctctt gcgggtaaaa tttcaaaaga tgaagaatgc tcccgccttg
      541 tccctgccat gaaaccgctt gcaggactcc ctgtacccat atccgtagat acgatgtttt
      601 ctgacatagc caaagaagcg cttgctgcag gcgctaatat cataaatgat gttagcggct
      661 tcacggctga tgacaaaatg atgggagtgg cggcaaaatt cgcctgtcct gtaatattga
      721 tggccagtaa taatattcca ggcgaccctg tgggaatgga tgccataatg gaatcccttg
      781 aaagaataat tgcatacgcc cagaataatg gaatttcacc ggattccatt ataattgatc
      841 ccgctattgg aaaatggact ccccggaaac ttgcgatcta tgattatgaa actattgata
      901 atatcgaaag acttcggatc tttaagaaac ccatactggc agcaatatcc cgaaaatcct
      961 tcatgggtga tactttgaac aaacccgcaa ctgataggct ttatggaagc attgccgcaa
     1021 cagctatcgc agtaagaaat ggggcgcaca taataaggac gcatgacgtt gcgccaactg
     1081 tggatgccgt aagggtcgca gaagcatcaa gagcaaggat tcccatagtc agatcaggaa
     1141 caattgaagc tgaattgctt gaaatcaaaa atattaagga ttgccagaaa gcaatgcttt
     1201 tgatcggagt gacgtcaaag ggaagtcaaa taatgaagaa aaagaccctg atatataaca
     1261 tcctgatcaa taatataagc acgaccgaag cattgataat caagcaggag atgctggcaa
     1321 gaggaggaga cgcagccctt ccgcgtgatg cggtatcaca tgaaacccag aaagtgagcc
     1381 ttatcatttc cggaacacag ctccaggttg aacgtcttat caataaaata cgccagcagg
     1441 taagagacct tccctcaatt gcagatatgc tgaccgaatt gatgaatcag aataacgata
     1501 ctgtttttag atattcaaga tcaaaatgat aatttcaagg caaaaagaat ttattgatat
     1561 attgcagctg cttgctgaaa aaaatatttt cataatcggc tgcggcaaat gcgccaaaaa
     1621 acagcgtgtt gggggggaac ctgaagtaaa ggatatgaaa aaacgactgg aagatgcagg
     1681 aaaaaatatt accggatgga ctgttttaag ttccgcatgc agcatcagtt cctggatgga
     1741 agtattatct atgaatccgg aaattgacaa atcggatgca cttctggtaa tgtcatgcgg
     1801 gggaggcgtt tctgtgattt cagggtttgc aggtatagag gtttatccgg ctcttgatac
     1861 tgaatccctt ggaggagtag tctgtaacga agtggtgaca gaacaatgcg gtatgtgcgg
     1921 ggattgcaca atatggttat atggggggat atgtcccgta atacagtgtg caaaaggatt
     1981 attaaatgga ccatgcggag gcgcacttga aggaaaatgt gagatcgagg aacgcttatg
     2041 cgcctgggat aagatatttg aaaggttaaa agagcttaaa aaacttgaaa atcttgaaaa
     2101 gatcaatgag ccaaaagacc acatgagaaa ggtaagggtg aaaacttgaa tttcatttca
     2161 aaattacgct ccgaaaaatt catgatcaca gcagagatca gccctccaaa aggcaccgat
     2221 acatcgccaa tgttaaggga ctgcgcccta t
//
LOCUS       DATGBN010000047         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_469401, whole genome shotgun sequence.
ACCESSION   DATGBN010000047 DATGBN010000000
VERSION     DATGBN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469401"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /gene="leuS"
                     /locus_tag="VKL21_01005"
     CDS             complement(<1..1392)
                     /gene="leuS"
                     /locus_tag="VKL21_01005"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMB44378.1"
                     /translation="MKEWYNPHEIDEKWQRKWSDAKIFEPEPDDRKKFFITIPYPYLN
                     GNLHAGHTRTFTIGDVIARYKRMLGYNVLFPMGFHATGTPIVGLAEQIEKKDPETIRV
                     YNQLHQIPLDVLDSIRTPEQIVEYFSKEDETAMKIIGFSIDWRRKFTTTDPHYKKMIT
                     WQFNLLRSKNKVVKGAHPVRWCPHDENPVEDHDILKGENATIIDYTLIKFKLDSDILP
                     CATLRPETVFGVTNLWVNPGILHLRVKVNNETWIVSKEAYDKLKFTDKNVELIGEIDV
                     IELIGKKVKNPVTGSLVWILPASFVSPENGSGIVMSVPAHAPYDYLALRDLKGKDLAK
                     YAITEDVSSIPLISLIKVPEYGKFPAVDAVSELKVKDQNDPRAEEATKLVYRREFHTG
                     VLNENTGKYAGMAVSKIKDVLTHDFIEQGIADIFYEFSEQPVICRCGATCVIKLVKDQ
                     WFLNYSDKSWKEQV"
BASE COUNT          394 a          356 c          297 g          515 t
ORIGIN      
        1 cacctgttct ttccaggatt tatcagaata atttaggaac cactggtcct ttaccaattt
       61 tatgacgcat gtcgccccac acctgcatat taccggctgt tcgctgaact catagaatat
      121 atccgctatc ccctgttcaa taaagtcatg cgtaagcaca tcctttattt ttgaaaccgc
      181 cattcctgca tatttcccgg tattttcatt caggacgcct gtatgaaatt cccgcctgta
      241 aacaagtttt gttgcttctt cggccctggg gtcgttctgg tcttttactt ttaattcgga
      301 aaccgcatct acagcaggaa attttccata ttcgggtact tttatcagtg aaatgagcgg
      361 gattgaggat acgtcttctg taattgcgta ttttgccaga tccttcccct ttagatcacg
      421 aagtgcaaga taatcgtatg gggcatgcgc cggaacgctc attacaatgc cgctgccatt
      481 ttcagggctg acaaaagatg ctggcagtat ccagacaaga gaacccgtga caggattttt
      541 cactttttta cctatcaact caattacatc gatttccccg attagttcca cgttcttatc
      601 tgtgaatttg agtttatcat aggcttcttt acttactatc caggtttcat tgtttacttt
      661 aaccctcagg tgcaatatcc cgggattgac ccacaggttc gttaccccga atacggtttc
      721 aggacgaagt gtggcgcatg gaagaatatc gctgtcgagt ttgaatttaa tgagggtata
      781 atcaataatt gtggcatttt cgccttttaa gatgtcatgg tcttcaaccg gattttcatc
      841 gtgcgggcac catctgacag gatgcgcccc ttttacgacc ttgttcttgc ttcgaagcag
      901 attgaactgc catgttatca ttttcttata atgcgggtca gtggttgtga attttcgtct
      961 ccagtcaata gaaaaaccaa tgattttcat agctgtttcg tcttctttgc tgaagtattc
     1021 aacgatttgc tcaggcgtcc tgatagaatc aaggacatca agcgggatct gatggagctg
     1081 attatatacc cgtatagtct cgggatcttt tttttctatc tgttcggcaa gaccaactat
     1141 tggtgttcca gtagcatgaa atcccattgg gaacaggaca ttatatccaa gcatccgttt
     1201 atatcgcgca ataacatcgc caatggtgaa ggtccttgta tgtcctgcat gaaggttgcc
     1261 atttaaatat gggtacggga tcgtgatgaa aaacttttta cgatcatcag gttcaggctc
     1321 gaatatcttc gcatcactcc atttgcgctg ccatttttca tctatttcat gaggattata
     1381 ccattctttc attattagct cttcactttg cacaacttat tactaaaaag ctttaacggt
     1441 ttgggctgaa tttataaatt acagcatccc catattcaaa caccttttca aacacatcag
     1501 gattttcctc aaaaagcccg ggtttaccaa atctttttgc ctcagcctct ccaaaatata
     1561 ca
//
LOCUS       DATGBN010000048         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_158753, whole genome shotgun sequence.
ACCESSION   DATGBN010000048 DATGBN010000000
VERSION     DATGBN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158753"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VKL21_01010"
     CDS             complement(<1..452)
                     /locus_tag="VKL21_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HMB44379.1"
                     /translation="MTLITESNIETFAIEVLKTLGWEYVHGLSIAPGAENAERESLDQ
                     IILTQRLRKAVAMLNHDIPSDAQEQAVQKVLRIYSPDMLHNNETFHQFLVEKVKIPYQ
                     QDGYERSYEVALIDFSNPSNNEFLVVNQYMIIENNQNKRPDILLFING"
     gene            complement(801..>1648)
                     /locus_tag="VKL21_01015"
     CDS             complement(801..>1648)
                     /locus_tag="VKL21_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44380.1"
                     /translation="LSRWKMKNITLVWDRGFVSKPNIDYAREKNFQVLSGGPHTSKEV
                     DDWITKYTDSEIEKRKNILEMSRGNGVYCIEDTGELFGRNCKIVVVLDPQRRNNSRIE
                     RDLLIQELESETSKKKIAELKKDLSPIVKPARGRRGYEINGDEEEVARRLDGRSLFFS
                     TDITMPGKQLIRTYFQKDHIEKAFRYLKGNACLAPIGYQLPNRVEAYLSVVNFISYEL
                     IAAILWKIEKFNLGIGYESLMEKASKIFEVEFSSKNSKLYRWTHISKEDEKLFKPFNI
                     LSVRS"
BASE COUNT          465 a          341 c          277 g          565 t
ORIGIN      
        1 ataccattga taaatagcaa gatgtcaggt cgcttgttct ggttgttctc aatgatcatg
       61 tactgattga caactaagaa ttcgttgttg gaaggattgc taaaatcgat cagcgctact
      121 tcatagcttc tttcatagcc atcctgctga tagggtattt ttactttctc aaccaggaat
      181 tgatgaaagg tttcattgtt gtgcagcatg tctggtgaat aaatccgtag cactttttgc
      241 acagcctgtt cctgtgcgtc cgagggaata tcatggttta acattgcaac agctttgcgt
      301 aagcgctgcg ttaaaatgat ctggtccaaa ctttccctct cggcattctc ggcgccgggg
      361 gcgatggaca agccgtgaac atactcccag cctaatgttt taagtacttc gatggcgaag
      421 gtttctatgt tgctttcggt gatgagtgtc attgttaaaa aaacgttaag ctaatcctct
      481 gcgttcttct attatcgcat cgcttaaaga ccccttatag ctttccagca gcttcatgga
      541 tttgttaaat gagaatttat ttaaatcgag atattctatt tttgtatcgc cttgaataat
      601 ttcccttcca gatccgcctt ttatataaaa cctgaatttc ggtggttttg gtaactatgt
      661 tttttatttt tgaatttcgc atgcagtaga accgttcaga gattaatttc ctccaatttg
      721 aaagcattat cttaaaaaca tggcaatgca tatcgataaa attcaaatca ttctcaaaaa
      781 acaaaaatct cgaaaatcaa ttagctgcga actgacaata tattaaatgg cttgaaaagt
      841 ttctcatctt cctttgaaat atgagtccac cgatacaatt tcgaattttt cgaagaaaat
      901 tctacttcaa atatctttga ggctttttcc atcaaacttt cataacctat tccgagattg
      961 aatttctcaa tcttccataa gatagcggca atcaattcat acgaaatgaa attaacaaca
     1021 gaaagatatg cttctacccg atttggaagt tgatatccga taggggccag gcaggcatta
     1081 ccctttaaat atctgaatgc cttttcaata tgatctttct ggaaatatgt ccttatcagc
     1141 tgtttccctg gcattgtaat atcggtggaa aagaacaatg atcttccatc aagtcttctt
     1201 gcaacttctt cttcatcccc atttatttca taacctcttc tccctcttgc tggtttcaca
     1261 atcggggaca agtccttttt caattctgca atcttctttt ttgaggtttc agactcaagt
     1321 tcttgaataa gtaagtctct ttcgatgcgt gaattattgc gtctttgcgg atccaacaca
     1381 acaacaattt tacaattccg tccaaataat tctccagtat cttcgataca gtaaacacca
     1441 tttccacggg acatttcaag aatatttttt cgtttttcga tttcagaatc cgtatatttc
     1501 gtaatccaat catcgacctc ctttgaggta tgaggtccac cagaaaggac ctgaaagttc
     1561 ttttctcttg cataatcaat gttaggttta ctgacaaaac cacgatccca tacaagcgta
     1621 atgtttttca ttttccaacg ggataaat
//
LOCUS       DATGBN010000049         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_469453, whole genome shotgun sequence.
ACCESSION   DATGBN010000049 DATGBN010000000
VERSION     DATGBN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469453"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VKL21_01020"
     CDS             complement(<1..503)
                     /locus_tag="VKL21_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HMB44381.1"
                     /translation="MLEIEKVKEIEKTEREKIKEGIKQKKEKEKVGQFEKATVECPEC
                     KSHALVHDYERAELICNECGLVVDADFIDQGPEWRAFDHDQRMKRSRVGAPMTYTIHD
                     KGLSTMIDWRNRDSYGKSISSKSRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMA
                     SALGLSQ"
     gene            complement(518..763)
                     /locus_tag="VKL21_01025"
     CDS             complement(518..763)
                     /locus_tag="VKL21_01025"
                     /inference="COORDINATES: protein motif:HMM:NF016308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HMB44382.1"
                     /translation="MKRLGTVLHSIDNMLIVRADKTLESSNLYPDSMVITKTMKRIGR
                     VKELFGPEKNPYISIKLFNEIKDSEKTKLKNERVYLA"
     gene            904..1575
                     /locus_tag="VKL21_01030"
     CDS             904..1575
                     /locus_tag="VKL21_01030"
                     /inference="COORDINATES: protein motif:HMM:NF039705.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HMB44383.1"
                     /translation="MEYIETICPSCSPDTPVNHIILKGIKDELLQCQECKSVHKEKKP
                     RNLLVRVIVSKGKESVHTRAVLSGLIRKDDEILIDDEATGEAYLVKITSIEVGDKRQD
                     EGNAQVIKTIWARAIDEAIVKFAISHRETTESVSMRVPGEREFVIGDKVEVNNRKLKI
                     VRIKIRDGGFKSRRGVAVKAKYIKRIYADSGIKEPKRFSRAKGERVVIKKRESVWSLR
                     SKGTG"
BASE COUNT          468 a          301 c          330 g          481 t
ORIGIN      
        1 ttctgtgata atcccagagc tgatgccatc ctgtcaagtt ctgatagggc aaaagcaagg
       61 ttcctttcgg tcgcattact tacgcgaata cggcgctgcc atttcctgag gcggtataac
      121 tgcgccctgc tcttactgga tatggatttt ccatatgagt cccggtttct ccagtctatc
      181 atggtggata atcccttatc atgaattgta taggtcattg gagcacccac acgagaccgt
      241 ttcattctct ggtcatggtc aaaagccctc cattccgggc cctggtcgat aaagtccgca
      301 tcaactacca ggccgcattc gttgcaaata agttccgctc tttcataatc atgtacaagg
      361 gcatggctct tgcattccgg gcactccact gtggctttct cgaactgacc gaccttctct
      421 ttttcttttt tctgcttgat gccttccttt attttttccc gttcggtctt ctcaatttcc
      481 tttacttttt caatttctaa cattatacac ctttccttta tgccaaatag actctttcat
      541 tttttaattt cgttttttct gagtctttga tttcattgaa taacttgatt gatatgtagg
      601 gatttttctc cgggccaaaa agttctttta ccctgcctat cctcttcatc gtttttgtaa
      661 taaccattga atccgggtaa agattacttg attcaagtgt tttatcggcg cggactatca
      721 acatattatc gatagaatga agaacagttc ccaatctttt caatttttac ctcagcgaaa
      781 gagttatata ttcagatatg tagtatataa acctatcgtt tgaattttaa tttgtcatgg
      841 aaaataatat cctgctccat ttttgacagg atatttatac ctttaaagcc ttaatttgaa
      901 tatatggaat atatcgaaac tatttgccct tcgtgctcgc cagatactcc tgttaaccat
      961 ataatcctga aaggaataaa ggatgaattg ttacagtgcc aggaatgtaa gtcagttcat
     1021 aaagagaaaa aaccaagaaa tttactggta agagtcattg tgagcaaagg gaaagaatcc
     1081 gtccatacaa gggcagtact ctccggattg atccgaaaag atgatgagat cctaatagat
     1141 gatgaggcaa cgggggaagc atatcttgta aaaataactt ctatagaagt tggggataaa
     1201 cgacaggatg agggaaatgc ccaggttatc aaaacgatat gggcaagggc gatagatgaa
     1261 gctattgtaa aatttgccat aagtcacagg gaaaccacag aatctgtttc tatgcgtgta
     1321 ccgggtgaaa gagaattcgt aataggggat aaagtagaag taaataacag gaaattaaag
     1381 atagtccgga taaaaataag ggatggcgga tttaagagta gaagaggtgt tgctgtcaaa
     1441 gcaaaatata taaagcgaat atatgctgat tccggaatca aggaaccaaa acgattttca
     1501 agggcaaagg gagagcgggt tgtaatcaag aagcgggagt cagtatggag tttgaggtca
     1561 aaaggaaccg gctgatcgaa
//
LOCUS       DATGBN010000050         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_158759, whole genome shotgun sequence.
ACCESSION   DATGBN010000050 DATGBN010000000
VERSION     DATGBN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158759"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VKL21_01035"
     CDS             complement(<1..1248)
                     /locus_tag="VKL21_01035"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMB44384.1"
                     /translation="MIKGTLLIVDDNKSVISALEMMLQYEVEKLISISSPKRIHEVLE
                     KNEIDIVLLDMNFQAGVNSGNEGLFWMKEILKHDSTISVVMITAYGDVELAVKTLREG
                     AIDFILKPWDNQKLLATINAAWKLRTSRKEAILLKNDKQFLKNEIKRGEEKIVLGASP
                     TMINVMNIVGKVAATDANVLITGENGTGKELVAREIHNQSKRSNELMVSVDMGSITET
                     LFESEMFGHVKGAFTDAKEDRSGKFEIAQKGTLFLDEIGNLSPQSQVKLLSALQNRFI
                     VRVGSNRQIPVDIRLICATNCILPIRVNEGLFREDLLYRINTIQIEVPPLRDRVDDIP
                     VLAFHFLRFYCEKYNRPAKKINNQALEKLSNYQWPGNIREMRHSIEKAVILSESSILG
                     PSDFSFNTSSKGVSYNDNTTLEEM"
BASE COUNT          429 a          318 c          245 g          527 t
ORIGIN      
        1 catttcttca agggtggtat tgtcattata tgaaactcct ttagaagagg tattaaaaga
       61 aaaatcagaa gggcccagaa tagatgattc tgagaggatc acagcttttt ctatagaatg
      121 tcgcatttca cggatatttc caggccactg gtaatttgaa agtttttcca gcgcctggtt
      181 gtttattttc tttgctggtc tattatattt ctcacagtag aatctaagaa agtgaaacgc
      241 cagcacgggg atatcgtcta ccctgtcccg taacggaggt acttcaatct gaattgtatt
      301 tattctgtaa agaaggtctt ccctgaacaa tccttcattt acccttatag gcagaataca
      361 atttgttgca catatcaatc tgatatccac cggtatctgt ctgtttgatc ctacacgcac
      421 tatgaatctg ttctgaaggg cgctaagcaa tttgacctgt gattgcggag aaagatttcc
      481 tatctcatcg aggaagagag ttcccttttg agcaatttca aatttacctg aacggtcttc
      541 ttttgcatct gtaaatgcac cctttacatg accgaacatc tcactctcaa aaagcgtttc
      601 ggttatagaa cccatatcca cgctgaccat aagttcatta cttctctttg attgattatg
      661 gatttccctg gcaaccagct ctttcccagt accattttct cctgtaatta acacattggc
      721 atcggttgct gctaccttcc cgacaatatt cattacatta atcattgttg gagatgcacc
      781 caggactatc ttttcttcac ctctttttat ttcatttttc aggaactgtt tatcattttt
      841 aagcaagatt gcttcctttc tcgaagtcct cagtttccac gctgcattta tcgttgcaag
      901 tagcttttgg ttgtcccacg gtttgagaat aaagtcgatt gcaccctctc ttaaagtctt
      961 caccgcaagt tcaacatcac cataagctgt aatcataact acacttatag tgctgtcgtg
     1021 tttaagtatc tctttcatcc agaaaagtcc ctcatttcct gaattaacac cagcctgaaa
     1081 gttcatatcc agaagtacaa tatctatttc attcttttcc agtacttcat ggattctttt
     1141 aggactgctt atactaatca gtttttctac ttcatactgc aacatcattt caagtgcgct
     1201 tataacgctt ttgttgtcat ctacaataag aagggttccc tttatcattt gtccattttt
     1261 taacaaaata ctgaaattta ataatatgta tcaaatacat gtcaaataat tagacacttt
     1321 ggtgtgagat tttaactaag gatattgata attaagcata aaatcccaaa tcgcttgaaa
     1381 atcaatttat ttaccccatc cccaagggga gtaaattgat tttcttcgct ccccttgggg
     1441 acggggtaaa cgaagaaaat caatggactt atttggattg tttcaaaaag tcaattttta
     1501 tttttatcag aaaataata
//
LOCUS       DATGBN010000051         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_314362, whole genome shotgun sequence.
ACCESSION   DATGBN010000051 DATGBN010000000
VERSION     DATGBN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314362"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..491)
                     /locus_tag="VKL21_01040"
     CDS             complement(87..491)
                     /locus_tag="VKL21_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMB44385.1"
                     /translation="MQEISNKGNKNISIDELYRYSTECGELQCHRLNIPDGTAGELII
                     NVHGNEKALTIEEVFVDRKFRHMGLGSKLLAFAETIAMASGVQNIELKPFSTDPLVSD
                     HKLREWYMERGYQPHGEKMHKKLNKEILEVMT"
     gene            complement(505..1830)
                     /locus_tag="VKL21_01045"
     CDS             complement(505..1830)
                     /locus_tag="VKL21_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMB44386.1"
                     /translation="MNLIRRAGCFAIIFAITLAIITMHAMAQETETTPLNSGDTAWVL
                     VSAALVMLMTPAVGLFYGGMVRKKNVLAIIMQSFIILAIISIQWVLFGYSLAFGPDKW
                     HGLIGGLEWIGLNGVGAAPNPDYAATIPASVFMIFQAMFAIITPALIIGAFADRMKFS
                     TMMVFTVLWATLVYDPVAHWVWGIGGWIRMLGALDFAGGTVVHITAGISAFVAAIIIG
                     KRLGFGNENMAPHNVPMTIIGGVLLWFGWFGFNAGSALAANGLAANAFIVTNTAAATA
                     ALTWTIVSWTHGGKPNAVGIITGGVAGLVAITPASGFVGPIAAIIIGIGAGFFCYFAM
                     HLRSKKTDIDDSLDVFACHGVGGIWGAIATGIFASIAVNSAGADGLIYGNPGLVVKQL
                     IAIVAVLVYSLLMTAIILKVLDATMGLRVKDEHEVEGLDVSLHGEKAYL"
     gene            complement(2076..2450)
                     /locus_tag="VKL21_01050"
     CDS             complement(2076..2450)
                     /locus_tag="VKL21_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44387.1"
                     /translation="MSEYHNHSFTDAARKAAITCTDCHMPDVINTNSKLRGALTTHNF
                     KSLNPENSIKYGADVMPNSCTYRCHQDKGADKTARANWAISAMSPVAAATTASATTTK
                     APAFELIFAVTALFIATLVRRR"
     gene            2990..3376
                     /locus_tag="VKL21_01055"
     CDS             2990..3376
                     /locus_tag="VKL21_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HMB44388.1"
                     /translation="MKFQGKSTRKYTGGRLIRSRSKKKFELGGEPANTHLGETIRRTV
                     KKLGGQLKVRLLRGQNAIVTDPSTKTSKTVKIETVTGNPANIHYVRRNIVTKGSIIKT
                     EIGSARVTNRPGQEGSINAVLITSTA"
     gene            complement(3357..4415)
                     /locus_tag="VKL21_01060"
     CDS             complement(3357..4415)
                     /locus_tag="VKL21_01060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMB44389.1"
                     /translation="MGLSLMYDVIVIGAGPAGSVTARTVALAGYDVLILEKKERCASP
                     CAGYISKTINIEMPDESVIQSNIKKMRTYFPDMTYHDFDLNGFVVDRPLFDKGLLSKA
                     IVAGADIMWDSPLVDMSLGQLNPGIVKFRNGNASGRIIVGADGVFSKTASIIGLQQQR
                     FAACSQYHLKDIEPIPQTCEIFFNADYAPGGYIWIYPTGKNSAKVGLGMINSKKSPRE
                     NLDAFIAGSRITHRISGKRTEYITGALPIGGLREKLVFDNVLLAGDSAGMADPITGAG
                     INNAMLAGELAGKTIIKALENDDIAQLLEYETGIKKLLGRPLERSLEKRKKIDACNNQ
                     LLQEHLPELWVTFKQYWL"
     gene            complement(4400..>4657)
                     /locus_tag="VKL21_01065"
     CDS             complement(4400..>4657)
                     /locus_tag="VKL21_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44390.1"
                     /translation="EMLRISNIPKEKFMDKKIASVREGLTWLDRELGEQRDMIEIQNV
                     MKKKFEERFHVRLISGPLTGEEKEFTNSLLPRYYSNEWVYR"
BASE COUNT         1290 a         1077 c          927 g         1363 t
ORIGIN      
        1 ggtcgtaagt ccaatgaatc ctgccccggc aagaactgtc tttatttcac cgagcttctc
       61 aggtcttatt atcgcttcta tctttttcat gtcatcacct ctaatatttc tttatttagt
      121 ttcttatgca tcttttctcc atgaggctgg taaccccgtt ccatgtacca ttcccgcagt
      181 ttatgatcgg atacaagagg atccgttgaa aaaggcttta gctcgatgtt ttgaactccg
      241 gatgccatgg caattgtttc tgcaaaagca agcagcttgc ttcctaaacc catatgcctg
      301 aatttcctgt ctacaaatac ttcttctatg gtgagcgctt tttcattccc gtgaacgttt
      361 atgatcagtt ctccagcggt accatcaggt atattcaatc tgtggcattg cagttccccg
      421 cattcggtac tgtacctgta aagctcatct attgatatgt tcttattacc tttgtttgaa
      481 atttcttgca tatccttctt ctccttacaa gtatgctttt tcaccatgca aggacacgtc
      541 aagcccttcg acttcgtgtt catcctttac tcttaggccc atcgttgcat ccagcacctt
      601 gagtataatg gcggtcatca ggagtgaata gaccagtaca gccacgatcg ctatcagttg
      661 cttgaccaca agtcccggat ttccataaat aagtccgtcc gctccggcag agtttacagc
      721 aatggatgca aatatcccgg ttgcgatagc accccagata cctccgaccc catggcatgc
      781 aaagacatca agagaatcat cgatatcggt ctttttggac ctcaaatgca ttgcaaagta
      841 gcagaaaaac ccggccccaa tgcctattat tatcgcagct atcggcccta caaaaccaga
      901 tgccggagtt atagccacaa gcccggcaac acctccggta atgatcccga ccgcattagg
      961 ttttcctcca tgcgtccagc ttactattgt ccatgtaaga gctgcagttg ccgcagctgt
     1021 atttgttaca atgaaagcgt ttgcagctag cccgttcgcc gccagggcgc ttcctgcatt
     1081 gaaaccgaac cagccaaacc acaaaagcac gcccccgatg attgtcatgg ggacattgtg
     1141 gggcgccatg ttctcatttc caaatccgag gcgcttgccg ataattatcg cagcgacaaa
     1201 agctgaaata cccgctgtta tatgaacgac agttccccct gcaaagtcaa gagccccaag
     1261 catgcgtatc catccgccaa taccccagac ccagtgcgcc acagggtcat agacaagagt
     1321 tgcccagaga accgtgaaaa ccatcattgt gctaaacttc atcctgtccg caaaggctcc
     1381 tattatgagc gccggagtga taatggcgaa catggcctgg aagatcatga ataccgatgc
     1441 aggtatagtg gcagcataat cagggtttgg cgctgctcca acaccattca ggcctatcca
     1501 ttcaagccct cctatcaatc catgccattt atccgggcca aatgccagac tgtatccgaa
     1561 tagcacccac tggatgctta ttattgccag tattataaag ctctgcatta tgatcgccag
     1621 gacattcttt ttcctgacca taccgccata gaaaagccca acagcgggcg tcattaacat
     1681 aaccagggct gccgaaacga gcacccatgc cgtatctcca ctatttaggg gtgttgtttc
     1741 agtctcctgt gccatggcat gcatcgtaat tattgcaagt gtgatggcaa aaatgattgc
     1801 aaaacatcct gctcttcgta ttaagttcat cagattcctc cttcatttta ataggtataa
     1861 ggtagcttac tgtagattta ataatatata aattttacta atagggtggg aaaagtatat
     1921 atataacatt tgattcggac taagaatcag aattattaat aaaaattttc agaaaatctt
     1981 ctcaaaaatc cacaaacttt aaaaaatact accctgaatg tccggcggat ttcctgaatt
     2041 caatattttg aaaaaaatag gaaagaagat tgaatttacc ttcttcttac aagtgtcgca
     2101 ataaacagcg ctgtgaccgc aaatatcaat tcaaaggctg gagcctttgt tgtcgtggca
     2161 gatgctgttg ttgctgctgc caccggactc attgctgaaa tcgcccagtt ggcacgtgct
     2221 gtcttatcag cgcccttgtc ctggtggcac ctgtaggtgc atgagttggg catgacatct
     2281 gcgccatatt taattgagtt ttcagggttc agggatttga agttatgcgt tgtcaatgcg
     2341 cccctgagtt tgctgtttgt gtttattaca tcaggcatat gacagtcggt acacgttatc
     2401 gctgccttcc tggctgcatc cgtgaagctg tggttatggt attcactcaa agattgcaac
     2461 gctcctgtca gcgaattcag caggcaattc cgataccggg tatacgatca cagcaccagc
     2521 atcatggggg ctgtgacatg atacgcatgt cgtttgggca gtgtagtgct tgctggtctt
     2581 gacatcaagg tactgctcac ggagtcgctt tgtcgtctgg tccgggaaga acgatgcatt
     2641 gttggtgtaa tttcgcggaa ttttaagcct tatcgcaaaa tctaaagtat aaggatggcc
     2701 tatatttgat tatgacaaac aattggatcc acggtataga aatataaaat tttaaactac
     2761 tttaacattt ttatgaattt aggcagtaag ttcataaaac aagttattat aaaataaaat
     2821 gtattttaaa ttgaaggaat ataaggcgtt gttttttata gtttcaagac aaattaagtt
     2881 gaaatataat agaggtctat aataaaagct tatactacta ttttatatta tattactgcg
     2941 tagagcggca aaataattat tctatcattt aagatcaaag aggatcatta tgaagttcca
     3001 gggaaaatca acacgaaaat atacaggcgg aaggcttata cggtcaagaa gtaagaaaaa
     3061 attcgaactt ggcggagagc ccgcaaacac tcatttaggc gaaactattc gcagaacagt
     3121 taaaaaatta ggcggccagc taaaagttcg actattaaga gggcaaaatg caatagtgac
     3181 agacccgtct acaaaaacct caaagacggt aaagatcgaa actgttacgg gaaatcccgc
     3241 aaatatccac tatgtgagac gaaacattgt gacaaaaggc tctattatca aaactgaaat
     3301 cggaagcgcc cgggtcacaa acaggccggg acaggaaggc agcattaatg ctgttctcat
     3361 aaccagtact gcttaaaagt cacccataat tccggcagat gctcctgcaa taattgatta
     3421 ttacaggcat cgattttttt tcttttttca agagaacgtt ccaggggcct gccaagaagt
     3481 ttcttgattc ctgtttcata ttccaggagt tgggcaatat catcattttc cagcgctttg
     3541 attattgttt ttcctgctaa ttcacctgca agcatagcat tatttatgcc ggcgcccgtt
     3601 atcgggtccg ccattcctgc gctgtcgcct gcaagaagga cgttgtcaaa tacgagtttt
     3661 tcacgaagcc ccccgattgg cagggcgcct gtaatatatt ctgttctttt gcctgaaata
     3721 cggtgtgtga tccgtgatcc tgcaataaat gcatcaaggt tctctcgcgg ggattttttg
     3781 ctatttatca ttcccaatcc cacttttgct gaatttttcc ctgtcgggta tatccagata
     3841 tatccgccgg gggcataatc cgcattaaaa aaaatctcac atgtttgtgg aataggttcg
     3901 atatctttaa ggtgatactg gctgcaggcc gcaaatcttt gttgctgcaa tccaattatt
     3961 gaagctgtct ttgaaaaaac accatctgca ccaacgatta ttcttccact tgcattaccg
     4021 ttacggaatt tcacaatacc aggatttagt tgtcccaaac tcatatcgac caggggtgaa
     4081 tcccacatga tatctgctcc tgccactatt gctttagaca acaagccctt gtcaaacaaa
     4141 ggtctatcca ccacaaatcc atttaaatcg aaatcatgat aagtcatatc aggaaaatag
     4201 gttcgcattt tttttatatt tgactgaata acagattcat ccggcatttc aatatttatg
     4261 gttttactga tatacccggc gcatggtgat gcgcatcttt cttttttttc aaggataagg
     4321 acatcataac ctgccagggc tactgttctt gcagtaacgg acccggcagg ccctgcacca
     4381 ataacaataa catcatacat tagcgataga cccattcatt tgagtaatac ctgggtagga
     4441 gagaatttgt aaactccttt tcttcccctg tcaggggtcc ggatattaaa cggacatgaa
     4501 agcgctcttc aaatttcttt ttcatcacat tttgtatctc tatcatatca cgctgttccc
     4561 cgagttccct gtcaagccat gtgagccctt cccttactga cgctattttt ttatccatga
     4621 atttttcttt cgggatattt gatatccgaa gcatctc
//
LOCUS       DATGBN010000052         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_236589, whole genome shotgun sequence.
ACCESSION   DATGBN010000052 DATGBN010000000
VERSION     DATGBN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236589"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VKL21_01070"
     CDS             <1..987
                     /locus_tag="VKL21_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HMB44391.1"
                     /translation="NSRRSLYFVFGLVTLVKVEELGKGNIVGHENIFDVNSNERYRLM
                     RSSMMNFSPIAAEYSMPDHDLNDIFEKYLGFKRPDYVPDPNTPPVIDVTLNGSRHFLW
                     EITDTKLIRKITKIVSGLKLLILDGHHRYTASNLLREKDGIEYTMMMLMEGGDRALLL
                     LPWHRAVRNFSEQELKQKIRENFSIDWESDTNDEEFYSRLRKRRSEFDIKLGMYDGKK
                     FSILRADELAVHELSRQKKEIVGLDLIVLHEWLIDPVIRGKPLDDVFFNASPDEAIAR
                     VDSKEYQVAFLLNPFSIKDVERKAFIEGKNFPQKSTLFLPKVAEGIVMRKIL"
     gene            complement(982..1164)
                     /locus_tag="VKL21_01075"
     CDS             complement(982..1164)
                     /locus_tag="VKL21_01075"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HMB44392.1"
                     /translation="MKFRVLIEQDEDGIFVASVPELPGCISQGKTRGEAIHNIKDAVE
                     GYLVSLKKHNEPIPLL"
     gene            1285..2385
                     /locus_tag="VKL21_01080"
     CDS             1285..2385
                     /locus_tag="VKL21_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HMB44393.1"
                     /translation="MHTLIICIDRDNDLGEKAQIGSPIIGRASNIDAAVKLASSDPED
                     SDINTIFGGINVYDKLIAQKEDVEIVSIAGDKKVGIKADKKIADQLDMILLELKPLSV
                     ILVSDGAEDESVLPLIQSRIKVDAVHRIIVKQHENLESTYYIIKKAFNDPKISHTFFV
                     PIGLVFLIYAISLYFARPEIAVMSITAAIGLYMLFRGTGLDDAVDSFKTSMMRSLYAG
                     KITFVMYLAAIILLLIGTVQGIIVSWDNYYNQPIWVGYLVLLMGYIYSSIWWYTLAGV
                     IVNIGIIFDLHMAKEKIGRQLSHPFFVLATGIILWAGSVYILVIREAMGTYPIFNNGR
                     QFVFGSLIVAVLIAVVGSWISTRTIRKKATKY"
     gene            complement(2382..2945)
                     /locus_tag="VKL21_01085"
     CDS             complement(2382..2945)
                     /locus_tag="VKL21_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44394.1"
                     /translation="MSPTIFHYKARSLLWGEDFEQVIIGIDPGKYPGIAVLGNKKTIS
                     VHHASVGEVCPLIKRIMREFDNKKIIVRIGHGARLIRSQLINSILDLGLQVEMVDETG
                     TTPRLGKGVHGQVISDIIAAINIALIPGNIVGKQFIEPSNGEVRVIQESSREYSNGHS
                     TIPRILARAVAKGELTLDEAVERHRND"
BASE COUNT          892 a          593 c          652 g          908 t
ORIGIN      
        1 aattccagga gaagcctgta ttttgtattc ggtcttgtca ctcttgtgaa agttgaagag
       61 ctcggtaaag ggaatatcgt ggggcatgaa aatatttttg atgtaaattc caatgagcgc
      121 tacaggttga tgagatcaag catgatgaac ttttcaccca ttgcagccga atacagcatg
      181 ccagatcatg acttgaatga tattttcgag aaatacctgg gttttaagcg cccggattat
      241 gtaccggatc cgaatacgcc tcctgttatt gatgttaccc tgaacggcag ccgtcatttc
      301 ttatgggaaa taactgatac aaaacttatt cggaagatca caaagattgt ttcgggcctt
      361 aaacttctca tactagatgg gcaccacagg tatactgcat caaatcttct acgtgaaaag
      421 gatggtattg aatatacaat gatgatgttg atggaaggcg gggacagggc gctcttactt
      481 ttgccatggc acagggcggt gcgaaatttc agtgagcagg aactaaagca aaaaataagg
      541 gagaattttt cgatagactg ggaaagcgat actaacgatg aagaatttta ttcccggctc
      601 aggaaacgca gaagtgaatt tgatattaag cttggcatgt atgacggaaa aaaattcagt
      661 atccttagag cagatgaatt ggctgtccat gagctttcaa gacaaaaaaa ggaaatcgtg
      721 ggtcttgacc tgatcgttct tcatgaatgg ttaattgatc ctgtaatccg gggtaaacct
      781 ttagatgatg tgttttttaa tgcaagtcct gatgaagcca ttgcaagggt ggattcaaag
      841 gaatatcaag ttgcttttct tttaaatcca ttctcaatta aagatgtgga gagaaaagca
      901 tttattgagg ggaagaattt cccccagaag tcaacgcttt tccttcccaa ggtggcggaa
      961 ggaattgtga tgcgaaaaat tttatagaag gggtataggc tcgttgtgtt ttttcaaact
     1021 aaccaaatat ccctcaacag catcctttat attatgaatt gcttcacctc tggtttttcc
     1081 ttgagaaatg caacctggca attccggaac agaagctaca aaaatcccgt cttcatcctg
     1141 ctctatcaag actctaaatt tcataatgtt aagatgtgtt ttgaataaat atattgatac
     1201 cgccaaggtc tgcgacttct tttaatgtta tttttgacgt tcattaggag gctttttata
     1261 taaacatcaa gataacagag tacaatgcat acgcttatca tatgtattga cagggacaat
     1321 gacctcgggg agaaggcaca gattggaagt cctataatcg ggcgcgcgtc gaacatcgat
     1381 gcggcggtca aacttgcctc ttctgaccct gaagattcgg atatcaacac aatttttggc
     1441 ggtataaatg tctatgataa actgatagcc caaaaagagg atgtggagat cgtttcaata
     1501 gcgggcgata agaaagtagg gataaaggcc gataagaaga tcgcagatca gcttgatatg
     1561 attctccttg agctaaaacc actcagtgtc atacttgttt ctgatggagc ggaagacgaa
     1621 tcggtactac ctctcatcca gtccaggata aaagtagatg cagtccacag aataattgta
     1681 aaacagcatg aaaatctgga aagtacgtat tatattatta aaaaagcctt caatgacccc
     1741 aagatctcac acacattttt tgttcccata ggtcttgtat tcctgatcta tgcgatctca
     1801 ttatattttg ccaggcctga gatcgcagta atgtcaataa ccgcagcaat aggcctttat
     1861 atgctcttcc gggggacggg tcttgatgat gcggtggaca gtttcaaaac atctatgatg
     1921 agatctttat atgccgggaa aattactttt gttatgtatc tggctgcaat cattctatta
     1981 ctaataggta ctgtacaggg aattatagta tcgtgggaca attattataa ccagccaata
     2041 tgggtgggat atctggtctt gcttatgggc tacatatata gttcaatctg gtggtatacg
     2101 cttgcaggag tgatagttaa cattggaata atctttgacc tccatatggc aaaagaaaag
     2161 atagggaggc aattgtcaca tccctttttc gtgttggcca caggcattat cttatgggcc
     2221 ggaagcgttt acatccttgt aataagggaa gcaatgggaa catatccaat tttcaataat
     2281 ggccgccagt ttgtatttgg aagcttgata gtggcggttc taattgcggt agtagggtca
     2341 tggatttcaa caagaacaat tagaaaaaaa gccacaaaat attaatcgtt cctgtgtctt
     2401 tccacagctt catcaagggt caattcccct tttgccacag ctcttgcaag aatacgcggg
     2461 atggtagagt gtccatttga atattcgcgg ctggattcct gaatgaccct aacttcccca
     2521 ttcgaaggct ctataaactg ttttcccacg atatttccag ggatcagcgc aatatttatc
     2581 gctgcgataa tatctgatat cacctgtcca tgaactcctt tccccagacg cggcgttgtt
     2641 ccggtttcat ctaccatttc aacctgtagt ccaagatcaa gtatgctgtt gatcagctgg
     2701 gaacggataa gtcttgcacc atgacctatc ctgacaatga tctttttatt atcaaattct
     2761 ctcattattc gttttataag cgggcatacc tcaccgaccg atgcatgatg aaccgaaatg
     2821 gttttcttat ttccaagtac agcaatacca ggatattttc cgggatctat tccgatgatt
     2881 acctgttcga aatcttctcc ccagagcaag gatcgcgctt tataatggaa aattgtcggt
     2941 gacaagagct atcgttttat acctttctaa ccctcctcgg aagttttttc taaaaatccc
     3001 ttgcctggaa tgaatctccc acaacccttt ttggcaatta tttcg
//
LOCUS       DATGBN010000053         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_158841, whole genome shotgun sequence.
ACCESSION   DATGBN010000053 DATGBN010000000
VERSION     DATGBN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158841"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VKL21_01090"
     CDS             complement(<1..311)
                     /locus_tag="VKL21_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMB44395.1"
                     /translation="MQSGTYFEKVNVNKKLTLRGIGNPVVNAGGSGSAITLSVNGIRL
                     EGFTATGGWGGVSRGDIRAGIKVTSKNNILIGNNASNNLHGIYLYYSSNNTLSRNIVS
                     N"
     gene            complement(477..1403)
                     /locus_tag="VKL21_01095"
     CDS             complement(477..1403)
                     /locus_tag="VKL21_01095"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HMB44396.1"
                     /translation="MELNKPIRHAKITRDMTVDQLVDALSGCAFGAGRIYEAVDIYRE
                     MINDRDCTKFLGLAGAMVPAGMRQIVSDMIRDREIDILVSTGANLVHDIIESMGLHHY
                     KGTDAIDDNKLKHEAVNRIYDVFLPEHHFTDFEEKLQSIFKEIPEKLSITELLSHIGA
                     RIDDNNSILKSAYDMNVPVYCPAIQDSIIGLQAWLYKQTKPLNVDVFEDMRGLIDRCF
                     DAKRAGVIIIGGGVPKNFILQSMLVTPRSFDYAIALTMDRPETGGLSGATLDEARSWG
                     KIGENARSVTVYSDATITLPIIVAAAKSGKIK"
     gene            complement(1439..>2541)
                     /gene="cfbE"
                     /locus_tag="VKL21_01100"
     CDS             complement(1439..>2541)
                     /gene="cfbE"
                     /locus_tag="VKL21_01100"
                     /inference="COORDINATES: protein motif:HMM:NF033197.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HMB44397.1"
                     /translation="LSHHQAVGHILSGYDLKNRTLIELTGTKAKTSTALLLADILSRE
                     RKVVSHTSRGLEDWSTGTILKKKGLSITPASILTALDHIQDAGIGFDVFIAEISLGGT
                     GFADIGIITTIANDYKIANNTRLASDAKRRMILDAKPRSKLVVNNDALRFFGACRRDI
                     DIISFTDSINATCNVYFESLDTKGATLAYFLGKEHGRFHIREASDYDITSYKTAFVCA
                     TAAALGMNIEARTVERTLLEFKGAEGRMKKKSLDGRILIDNSNSGMDIRTAKKALEYS
                     KKGDERIVMVLGEEAKEVCEGLDPTGVDLFINDHLHELAALVLVGDRMKPLAVKDLRK
                     IHHANDLSGGIELAKSLTKEKDIILSCVKCFR"
BASE COUNT          645 a          616 c          486 g          794 t
ORIGIN      
        1 ttgttcgata caatgtttct gctaagcgtg ttgttgctgg aatagtacag atatatgccg
       61 tgaaggttgt tcgatgcatt gttaccgatc aatatgttgt tttttgaagt gaccttgatc
      121 cctgccctta tatccccacg agagacacct ccccaacccc cggtcgctgt aaatccttca
      181 agtctaattc cattcacaga aagtgtaatc gcacttccgc ttcctccggc gttcactaca
      241 ggattgccaa taccgcgaag ggttagcttc ttattcacat tcactttttc gaaataagta
      301 ccgctttgca ctaaaatagt atcaccaatg cttgaggcgt taatcgcttt ctgtatgttt
      361 gagtatgcgc atccgccggg gcaaacctca agggtcgctg catttgcgct aaatcaggaa
      421 atattagaac ctttagaaat gatgaaaaat gtaggcaaaa taggtacaac cccctcctat
      481 ttaattttcc cgctttttgc cgctgcaaca attattggga gcgtgatcgt agcatcagaa
      541 tacactgtca cagaccttgc attttcccct atctttcccc atgagcgcgc ttcatcaagc
      601 gttgcgcctg aaagcccgcc ggtctcaggc ctgtccatgg tcagcgcaat ggcataatca
      661 aaagaacgcg gagttacaag catggattgc agtatgaagt tctttggcac gccgcctcct
      721 attatgatca ctccggcgcg ttttgcatca aaacacctgt ctatcaatcc tctcatatcc
      781 tcaaacacat ctacattcag cggctttgtc tgcttataca gccatgcctg taagccaatg
      841 atagaatcct ggatggcagg acagtaaaca ggcacattca tatcatatgc gctttttaag
      901 atggagttat tatcatcaat tcttgcaccg atgtgggaga gaagttcagt tattgaaagc
      961 ttctcaggaa tttctttgaa aattgattgc agtttttctt caaaatcagt gaaatgatgc
     1021 tctggcagga atacatcata aatacgatta acagcttcgt gttttaattt gttatcgtct
     1081 attgcatctg ttcccttgta atgatgaagc cccatagact cgattatatc atgaacaaga
     1141 tttgcgcctg tgctcaccag aatatcgatc tccctatccc ttatcatatc gctcacaatt
     1201 tgtctcattc ccgcaggcac catagcgccg gcaagaccca ggaattttgt gcaatcccgg
     1261 tcatttatca tttcccggta aatgtcaacg gcttcataaa tgcggcctgc tccgaaagcg
     1321 caccccgata aagcgtccac aagctgatct acggtcatat cccgcgttat tttcgcgtgc
     1381 ctgatgggtt tgttaagttc catatttgtc tattagttat cttttaattg atttaacttc
     1441 acctgaaaca tttcacacag gataaaataa tatccttctc ttttgttaat gattttgcaa
     1501 gctcgatccc gccagataga tcatttgcat gatggatttt tcttaaatcc ttcactgcta
     1561 acggtttcat tctgtcccct acaagcacga gggcggcaag ttcatgcagg tggtcattga
     1621 taaacaggtc aacaccggtt gggtcaagtc cttcacatac ttcttttgct tcctcgccaa
     1681 gcaccatcac gatcctttca tccccttttt ttgaatattc aagcgccttt ttggctgtcc
     1741 tgatatccat tcctgagttt gagttgtcga tcaatatcct tccatcaaga gatttcttct
     1801 tcatgcggcc ttctgcaccc ttgaattcaa gtaatgtacg ctcaactgtc cgcgcttcga
     1861 tattcatgcc aagagctgca gcagtcgcac acacaaaagc agttttataa gaagttatgt
     1921 cgtaatctga agcttcacgg atatggaacc tgccatgttc ctttccaaga aaataggcta
     1981 atgttgcgcc ttttgtatcc aggctctcaa aatagacatt acatgttgcg tttatggaat
     2041 cagtaaatga gataatatca atatctctcc tgcatgcccc gaaaaatctc aatgcatcat
     2101 tgtttacaac aagtttgctc cggggttttg catcaagtat catccttcgc tttgcatcac
     2161 tagcaagcct tgtgttattt gcgattttat aatcattggc tatagtggta attataccaa
     2221 tatcggcaaa acctgtcccg ccaagtgaga tctcagctat gaatacatcg aacccgatcc
     2281 ctgcatcttg aatgtgatca agagctgtaa ggatacttgc gggcgttatg ctcaatcctt
     2341 tttttttaag tattgttcct gtactccagt cttcaagacc cctgctcgtg tgcgagacaa
     2401 cttttctttc tctggataaa atatccgcca gcaggagcgc tgtgcttgtt ttggcttttg
     2461 tacctgtcag ctcgatcagt gtcctgttct tgaggtcata ccctgataag atatgcccca
     2521 ctgcctgatg gtgggataaa a
//
LOCUS       DATGBN010000054         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_275460, whole genome shotgun sequence.
ACCESSION   DATGBN010000054 DATGBN010000000
VERSION     DATGBN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275460"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VKL21_01105"
     CDS             complement(<1..740)
                     /locus_tag="VKL21_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMB44398.1"
                     /translation="MNSSIQIGKIMGIPIKLHITFLLIIPVFGYIFGNNPPEFGFSDI
                     EPLWLRYTLGLSVALLLFACVLLHELGHSYVAKKHGSNIQGITLFLFGGVSSLEDIPR
                     NPKIEFKMALAGPAISLLIGSILIIFYTLLNGDLHKSNPYLRMVGMMGYINVVLCIFN
                     LIPAFPMDGGRVLRAVLAGRMSYLKATRTAAYVGKMFSIFMGILGFLTVSSGGFWFML
                     IAFFIYIGASEEEKSTQVSVTLENVKIN"
     gene            937..2850
                     /gene="lonB"
                     /locus_tag="VKL21_01110"
     CDS             937..2850
                     /gene="lonB"
                     /locus_tag="VKL21_01110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HMB44399.1"
                     /translation="MENTENEVKNQELLGGLDISSTKDIEVPKKMIDQVIGQEHAVEV
                     VRKAANQRRHVMMLGTPGTGKSMLAKAMAELLPKEELQDVLVYNNPDDSNNPKIRIVP
                     AGKGKEITDAHKVEAQKRTTARNMFIMLLVLGIVGYSFFVGEYLMGIIAAVLIAMMMR
                     QFMPKETALIPKLLVSNKSKEIAPYIDATGAHAGALLGDVRHDPFQSGGLETPAHDRV
                     ESGAIHKAHKGVLFIDEINTLRIESQQNLLTALQEKEFAITGQSERSSGAMVKTDPVP
                     CDFIMVAAGNLDAIKGMHPALRSRVKGYGYEVYMSDTIEDTPDNRQKLVRFVAQEVVR
                     DGKIPHFDKGAVEEIIREARRRAGRKGHLTLKLRDLGGLVRVAGDIAHGENAALTTAA
                     HVLKAKSIARSVEQQLAYQYLERRKDYKLYIKEGAQVGRVNGLAVMGEDSGIVLPIIA
                     EVTPTQSTSEGHIIATGMLKNIAREAVQNVSALIKKTTGKDISKMDVHIQFVGTYEGV
                     EGDSASISIATAVLSAIQGIPVDQTVAMTGSLSVRGDVLPVGGVTYKIEAAALAGIKT
                     VLIPKSNMGDVLIEDEYKDLIKIIPVSNIEEVLEYSLVGGEKDGLIATLKKFASRTKF
                     SIPIPEPKPISAV"
     gene            2858..4186
                     /locus_tag="VKL21_01115"
     CDS             2858..4186
                     /locus_tag="VKL21_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMB44400.1"
                     /translation="MDFYDTRIIKSVSTSIILDNGEIREISNNFSSGGAVRVLDAGSW
                     GFVSMDGVHDDLNAAISSAGKLAESARNRSPRNPIHLAPIKEPSLRDLPEIKEKPQDI
                     PIEDKVKLLMEIEKNAKIDGIQSTNAIYSESLINVKYSSSEGLDCEYTLTRVGFAISA
                     IAQSEGIYQIGRESRFGVMGFELFKKHDAFELAQKAANTAKELLSAKTPKAGTYHVIL
                     DQELAGVFIHEAVGHAVEADHVLEGNSILSGKIGEQIASPLITVYDDPSLHEYGYYPF
                     DDEGAQSKRTTLIQKGILKSFIHSRETAGKLGGESRNSRAQGYSRPVIRMSNTFIAPD
                     GMKFDEMLSELKDGIYLKGSRGGQVNPGEGVFQFNAERGFIVKNGELTTSLRDVSLSG
                     NTLEILNNVSAVGNDLELNSGRCGKAGQLVPVSDGAPHLLVNRAMVGGSG"
BASE COUNT         1383 a          906 c         1050 g         1104 t
ORIGIN      
        1 tcattaattt ttacattctc aagcgtaaca ctgacctgtg tggatttttc ctcttccgat
       61 gcgccgatgt atatgaaaaa tgcgatgagc atgaaccaga aacctccgga ggaaactgtt
      121 aagaatccga gaatgcccat aaatattgaa aacatcttgc ccacatatgc agccgtacgt
      181 gttgctttca ggtaggacat cctgcctgca agcactgctc ttaacactct tccgccatcc
      241 attggaaatg cagggatcaa attgaatatg caaagaacaa cgttaatata tcccatcatt
      301 ccgaccattc gcagataagg attactctta tgcagatcgc cattaagaag tgtgtaaaaa
      361 atgatgagta tggaaccaat taaaagactg atggcgggtc ctgccaatgc catcttgaac
      421 tctatttttg ggtttctggg tatatcttca agtgaagaaa cccccccaaa aagaaaaagt
      481 gtaattccct ggatattgga gccgtgtttc ttggcaacgt acgaatgacc cagttcatgt
      541 aatagaacac atgcgaaaag caggagcgct acggataacc ccagcgtgta tcgaagccat
      601 aacggttcaa tatcactgaa gccaaactcg ggcggattat ttccaaaaat gtatccgaat
      661 accggtatta tgagcaggaa tgttatgtgc aatttaatcg ggatacccat tattttccct
      721 atctggattg atgaattcat gaagtcctct cttcttgtct ttattccctt tttattgatt
      781 tactgcgttt tataatttat tgtaatgagt tcgcaacctt ctttttggat gtagtggcga
      841 aaaagatata aaggtatatt cttattatca atatttctaa aaatgtacga taatattaag
      901 cctaataaac taattactta taatatcagg taaaacatgg aaaatactga aaacgaagtg
      961 aaaaatcaag agcttcttgg cgggttagat atatcatcta ctaaagatat tgaggttcct
     1021 aaaaaaatga tcgatcaggt cataggccag gaacatgccg tagaagtagt acgtaaagct
     1081 gcaaaccagc gaaggcacgt aatgatgctt ggaacgccag gcacaggtaa atcaatgctt
     1141 gcaaaagcca tggctgaatt acttcctaaa gaagaattgc aggatgtact ggtatataac
     1201 aatccggatg attcgaataa tccaaaaata cgtatagtac cggctggaaa aggtaaagag
     1261 atcactgatg cgcacaaagt ggaagcgcaa aaacgaacca cggcccgaaa catgttcatc
     1321 atgctgctgg tccttggaat cgtgggatat tcgtttttcg ttggtgaata tctcatggga
     1381 attatagctg cggtattaat agctatgatg atgcgccagt tcatgcccaa agaaacagct
     1441 ttgatcccaa agcttcttgt ttcaaataag agcaaagaaa tagctcctta tattgatgca
     1501 accggcgctc atgcaggcgc gcttcttggt gatgtgcgcc atgatccctt ccagtcaggc
     1561 gggcttgaaa cgcctgcgca tgaccgggta gagagcggtg cgatccataa agcccataag
     1621 ggtgttttgt tcatcgacga gataaacacg ctcaggatcg aatcacagca gaatcttttg
     1681 accgcgcttc aggaaaagga atttgcaatt acaggacaat ccgaacgttc cagcggcgct
     1741 atggtcaaaa ccgaccccgt cccgtgtgat ttcataatgg tcgcagccgg gaaccttgac
     1801 gctatcaagg gaatgcaccc tgcgcttcgc tcccgtgtca agggttacgg atacgaagta
     1861 tatatgagcg ataccataga ggataccccc gataacaggc aaaagcttgt caggttcgtt
     1921 gcccaggaag tcgtcagaga cgggaagatt ccacattttg acaaaggcgc agttgaagag
     1981 attatccgtg aagcgcgccg tcgcgcggga agaaaagggc atctgacact caaactcagg
     2041 gatcttggag gtcttgttcg agtcgccggg gatatcgcac acggggaaaa tgcagcgctc
     2101 acaaccgccg cgcatgtatt gaaagcaaag agcatagccc gttccgttga acagcagctt
     2161 gcttaccaat atcttgaacg aagaaaggat tataagctct atataaagga aggcgcacag
     2221 gtgggaagag tgaatggtct tgctgtcatg ggtgaggatt caggtatagt ccttcccata
     2281 attgcagagg tcacgcccac ccaatccaca tccgaaggcc atataatagc tacagggatg
     2341 ctgaaaaata tagcaagaga ggctgtgcag aatgtttcag cactgatcaa gaagaccaca
     2401 ggcaaggata tatcaaaaat ggatgtccat atccagttcg tgggaacgta tgaaggtgtg
     2461 gaaggcgatt ccgcaagcat ttcgatagct actgcagtac tctcagcaat acagggaatt
     2521 cctgtggatc agaccgtagc aatgacagga tcactttcag tcagaggcga tgtgttgccc
     2581 gttggcggtg taacatataa aatagaggca gcggcgctgg cagggataaa aactgtcctg
     2641 atcccgaaat cgaacatggg agatgtgctc atagaagatg aatataagga tttaataaaa
     2701 ataatccctg tgtcaaatat tgaagaggta ttggaataca gccttgttgg aggagaaaag
     2761 gatggtctta tagctaccct gaaaaaattc gcttcaagaa caaaattctc tatccctatc
     2821 cctgaaccaa agcctataag cgcagtgtga gaaacccatg gatttttatg atacgcgtat
     2881 tataaagagt gttagcacat ctattattct ggacaatggc gagattcgcg agatatcgaa
     2941 taatttttca agcggagggg ctgtaagagt tcttgacgcg ggttcatggg gatttgtttc
     3001 catggatggc gttcatgatg atcttaatgc cgctatttcc tctgcgggaa aacttgccga
     3061 atccgcaaga aacagaagcc caagaaatcc catccatctt gcccccataa aagaacccag
     3121 ccttcgggat cttcctgaaa taaaggaaaa accccaggac attcctattg aggataaagt
     3181 taaattactt atggaaattg aaaaaaatgc aaaaatagac ggaatacaaa gtacaaatgc
     3241 catatattcc gaatccctca taaatgtaaa atattcgagt tcagaagggc tagattgcga
     3301 atatacgtta actcgagttg gttttgccat cagcgcaata gcacaatcgg aaggcattta
     3361 ccagatcgga agggaaagca ggttcggggt aatgggattt gagctcttta agaagcatga
     3421 tgcttttgaa cttgcacaaa aagcagccaa tacagctaaa gaactacttt cggcaaaaac
     3481 gcctaaagcg ggaacatacc acgtgatact tgaccaggag cttgcgggtg tttttatcca
     3541 tgaggcggta ggtcatgcag tagaggcaga tcatgttctg gagggaaatt ccattctttc
     3601 gggaaaaata ggtgaacaaa tagcatctcc tcttattacg gtttatgacg acccttccct
     3661 tcatgaatat ggatattatc cttttgacga cgaaggcgcc caatcaaaga ggaccacatt
     3721 gatacagaag ggaatcctga aatcttttat ccattcaagg gaaacagcgg gaaagcttgg
     3781 cggtgaatcc aggaattcac gcgcccaggg ttactcaaga cccgtgatca ggatgagcaa
     3841 tacgttcata gctcctgatg gaatgaaatt tgatgaaatg ctctcagaat taaaagatgg
     3901 gatatatctt aaaggctcgc gcggcggcca ggtgaatccc ggtgaaggag ttttccagtt
     3961 caatgccgag cgcgggttca ttgtcaagaa cggggagctt acaacatcac ttcgggatgt
     4021 ttcactctca ggaaatactc ttgaaatatt gaataatgta tctgcggttg gaaatgacct
     4081 tgaattgaat tcaggaagat gcggaaaagc aggacagctt gtgccagtaa gtgacggagc
     4141 cccgcatttg cttgtgaaca gggcgatggt tggcggaagc gggtgaatag aataatcgct
     4201 cgatgaacct ctggttagaa caacgaacta atgcgaactg aaacgaactc tggaagtgcg
     4261 agttcgtatt aagttcatcc gagttagttg attctcattc gtcatcggtt aagagaaaaa
     4321 tcgtgagata gaacacggat tgtgcggatg acgcggatac acacggatcc tcccaccaac
     4381 gccagcccca aaaagggaaa agggtaaggg ggaaaccgga aattcatcat aactccattt
     4441 ttt
//
LOCUS       DATGBN010000055         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_508691, whole genome shotgun sequence.
ACCESSION   DATGBN010000055 DATGBN010000000
VERSION     DATGBN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508691"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VKL21_01120"
     CDS             <1..206
                     /locus_tag="VKL21_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44401.1"
                     /translation="SNMSDDTIREKKREQKLRFIEILLIVGGIISSLGLQYKSEVFVT
                     ILGFFSMAGHLHSNYSTSRLYSR"
     gene            complement(186..503)
                     /locus_tag="VKL21_01125"
     CDS             complement(186..503)
                     /locus_tag="VKL21_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMB44402.1"
                     /translation="MSEDTSIRVMKCSNCGKIVKERSNFCPYSSRKVDMDRLIIKEID
                     KGKDNLIISIVVPTMALGLLYENKNMYIAIFAFLILIAYLIFNIYIIFEAYRIAKLTT
                     IKS"
     gene            complement(657..791)
                     /locus_tag="VKL21_01130"
     CDS             complement(657..791)
                     /locus_tag="VKL21_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44403.1"
                     /translation="MAEQVTDTSERKLEEAAAAKLKKESEAKAAGAAEKSEMHKKVEK
                     "
     gene            complement(1103..1318)
                     /locus_tag="VKL21_01135"
     CDS             complement(1103..1318)
                     /locus_tag="VKL21_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44404.1"
                     /translation="MEEESKQINKTLNDLTVKIDNKIEELSKSIEKTVGEKQEYAEGK
                     ISENPLAYVAGAFIGGAIVGYVMGRGK"
     gene            complement(1464..1634)
                     /locus_tag="VKL21_01140"
     CDS             complement(1464..1634)
                     /locus_tag="VKL21_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44405.1"
                     /translation="MKNMAYKEPEVKGKESEAKRKLQVEAEEAKAKGAKAEETKAEET
                     KAEGEEAEVEEA"
     gene            complement(2096..2701)
                     /locus_tag="VKL21_01145"
     CDS             complement(2096..2701)
                     /locus_tag="VKL21_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMB44406.1"
                     /translation="MSKTTALKYLDSMKEKNLVDGEEIGTAKIWFLKTEEEIVQNEAH
                     EGRAVGNLMTPMATEVACTVPSLSRAAECNNKIKVLVADDDKDVIDIIRYSLDSDQFE
                     VLEAANGKEALGMVFARSPDILILDIMMSEMDGYMVCEELKEHDSTKNLPIIILSAKT
                     SVDDKIKAMSLDIDDYMTKPFDPRELEARIKMRLKQTASKN"
BASE COUNT          880 a          632 c          458 g         1202 t
ORIGIN      
        1 atagtaatat gagtgatgat acaatcagag agaaaaaaag agagcagaaa ttaagattta
       61 ttgaaatttt attgattgtt ggtggaataa ttagtagttt aggactacaa tataaatctg
      121 aagtttttgt aacaattctg ggtttcttta gtatggcagg acacttgcat tcaaattata
      181 gtacctcaag actttatagt cgttaatttt gcaattctat atgcttcaaa aataatatat
      241 atattaaata ttaaatatgc aatcagtatc aagaatgcga atatcgcaat gtacatattc
      301 ttattttcat ataataaacc taaagccata gttgggacaa ctatcgatat tatcaaatta
      361 tctttaccct tatcgatttc tttaattatc aatctatcca tatccacttt cctgctacta
      421 tacggacaga agttactcct ttcttttaca atttttccgc aatttgaaca tttcataact
      481 ctaattgaag tatcctccga catgtcagac tcctatgaat tttaacggtc tcattgttct
      541 gactcattcg acatactaat aaatctgaaa ttttaaattt ccatctttga aatgtgggtt
      601 aactaaaaat acagaaacca atagactttt tacatctatt ggtgatctga agttatctat
      661 ttctctactt tcttatgcat ttcactcttt tcagcagctc ctgctgcttt ggcttcagat
      721 tcttttttta atttagctgc tgccgcttct tccaatttac gttcgcttgt atccgttact
      781 tgttctgcca tatttttcaa ctcctttgat ttctttcttt atttaatttc tgaccaaata
      841 gtttccccac ttggtcttta gaaaaaataa aataggaaaa ttattagaca tataaacttt
      901 gctatcgatt ataaaggtgt acgcccttcg atgattctga gcagtatcaa gatgatagca
      961 ataaccagca atatatggat taagccacca actgcaccga gcgaaaatcc caatacccat
     1021 aggatcaaca gtatcactac taatgtccat attagatcca ttatctaccc tccttaatat
     1081 gattttacta tactttgctg tttcattttc ccctacccat cacgtaacct acaattgcac
     1141 cgccgataaa tgctcctgcc acataagcaa gagggttttc gcttattttc ccttcagcat
     1201 attcttgttt ctccccaact gtcttctcaa ttgatttaga tagttcctcg attttgttat
     1261 caattttgac tgttaggtca ttgagtgttt tatttatctg ctttgattct tcttccataa
     1321 attaacctcc acaatgtaat attatgtaaa gtatatacgt tattgaatgt cagccagtat
     1381 gttttttcgt ttcaaaaaat atagaaacca atagattttt tacatctatt ggcatcctct
     1441 cctgttctga agctatatat ttcttaagct tcttctactt cagcttcttc tccttcagct
     1501 tttgtttctt cagcttttgt ttcttcagct tttgctcctt tagcttttgc ttcttcagct
     1561 tctacttgca gttttctttt tgcttcggat tcttttcctt ttacttcggg ttctttatat
     1621 gccatatttt tcattcctta ttatagtctt ccgcataact tctgagaatt ttaaacaacg
     1681 aactaatacg aactatacga attaatacga actcgtgagt tcgttgcagt tagttgtttt
     1741 tatcatacgt tcggcgcaga actatatatt atataattaa gcaactctcc cacgcttaat
     1801 taatcataat tactctctta aactagttaa actttacgat cgttaataaa tgaatgaacg
     1861 atcgggaagt tttaagtact atggaggcat tcattgtatt gtttaccagg gagtaaacta
     1921 ccagagtggg aatttatcgt gccttcctcc tgtatcagcc acctattcaa gtcaaacaat
     1981 ccattgtatt gggaaggcac attccttata ttttaaattt ctattttgaa aacagtaggc
     2041 aaagaagatg gaagaattaa tagataaact ggcaggattt gaaaaaataa ctatctcaat
     2101 ttttactggc agtctgtttc aacctcattt ttattcttgc ttctagttct cttgggtcaa
     2161 acggttttgt catataatca tctatgtcta agctcattgc ttttatctta tcatcaacac
     2221 tggtctttgc agacaggatt attattggca aattctttgt agaatcatgt tctttcaatt
     2281 cttcgcagac catatagcca tccatttcag acatcatgat atctaagatc aggatatcag
     2341 gtgatctggc aaagaccatc cccaaagctt cttttccatt tgcggcttct aaaacctcga
     2401 attgatcgct gtctaaagaa tatcggataa tgtctataac atccttatca tcatcagcaa
     2461 ccaatacttt tattttattg ttacactccg cagctctgct gagactgggc acagtacatg
     2521 caacctcagt cgccattgga gtcatgagat tccccacagc tctgccttcg tgagcttcat
     2581 tttgaactat ttcctcttca gtttttaaaa accagatttt tgcagtacct atctcttcgc
     2641 catccaccag gtttttttct ttcatactat ccaggtactt cagggctgtt gtcttgctca
     2701 tatttattct gctaactatc tcgggagttg tcaattttcc ttgtttcagt atgttaagca
     2761 attctttttc acgccagtgc atattcgacc tttatgtttg ttcaaattta gatgatcata
     2821 tattggttta tggctaatta atctttgcat tgagtctctc aggctttttt ggtcggctga
     2881 tcatccaatt attaattggc gtacaattgg attaggtatg ttcaagggta tagatgttcc
     2941 atataaaatc caatacagga gtccaaagat ttcgtttaac aaacgatagt tctcattcat
     3001 gcggtctatt gcttggattt ctatacttta tatcttctag gcatttgttt tattgcttag
     3061 gatgttccac cagttcaatc agcacactgt tcgggtcttt gaaggaagca attttcattc
     3121 ccccttctac ttgtattggc tccaagacaa actcgactcc tttttcttta ag
//
LOCUS       DATGBN010000056         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_547458, whole genome shotgun sequence.
ACCESSION   DATGBN010000056 DATGBN010000000
VERSION     DATGBN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547458"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VKL21_01150"
     CDS             complement(<1..935)
                     /locus_tag="VKL21_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMB44407.1"
                     /translation="MKQVYMDHGSASPVDVRVLEAMIPFFENDIGNPSSLHSQGRKAK
                     RELENARTSVAKLIGAPNPKSIIFTSCATESNNLALRGAAMRYKDKGNHIITTSIEHM
                     SVMNALKDLQKNGFEVTYIPVDKDGLLDPGQVKNAITDKTILISVLYASGEIGTIQPI
                     REIGEIASERKIMFHVDGTAACSKVSVDVEKEHIDFLTISSNDMGGPKGVGALYIKQG
                     VRIIPFMLGGGQEFGMRSGSENIPGIVGMGKASLIAREEMTDESARLMKMRDKLIDNL
                     LKMEYTYLTGHRTKRLPNNASFRFSFIEGESIILQL"
     gene            complement(964..1125)
                     /locus_tag="VKL21_01155"
     CDS             complement(964..1125)
                     /locus_tag="VKL21_01155"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44408.1"
                     /translation="MKIQWKCSMCGYQFEGKATPPETCPSCKEACTFIDATCYIPECE
                     GPEGRDRRI"
     gene            complement(1135..1494)
                     /locus_tag="VKL21_01160"
     CDS             complement(1135..1494)
                     /locus_tag="VKL21_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMB44409.1"
                     /translation="MTNLLYVQTSGIDTPERLYSPFILAQTAKAMGIDAIIYFLGMGI
                     TVVKTGNAEKIKIGKFPTLKEVMDQTAKAGVKLMVCEQSTQLINLTRGDFIKEAEIVG
                     AATLNDLVLEADGTMWF"
     gene            complement(1632..2378)
                     /locus_tag="VKL21_01165"
     CDS             complement(1632..2378)
                     /locus_tag="VKL21_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44410.1"
                     /translation="MLDALENSREMILAIRKNTLDLDSIEKSIKENEGKSVSLKKEIE
                     EKQNDLNLLLDSESWKQYTISRNDLVLLETKAENKVSEIKSIISPLNKALNRLKQLNE
                     SGRHALKPEDKERLNLSLSCPTDVPPEFFVEFQKIVESGVLNLPKPEKFLPQIRFAAS
                     SLGESRKEYHDILHDIELKKDEISNMKIIKEEKNLKDMLSDLQEKLIITEKELDTSRN
                     QLESLKQEIESNNVKLREYVAVIDSRVRIS"
     gene            complement(2487..2882)
                     /locus_tag="VKL21_01170"
     CDS             complement(2487..2882)
                     /locus_tag="VKL21_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44411.1"
                     /translation="MNLIKKLFGKKEKSSGSITQDMLPEWLESRSRNISEKLEKDASS
                     LYPEIEKTLRKIKESTSRLEKAQPEGRYHLKIVKVASSNRDNMAKQVRMLLENLTIPK
                     DKDIKAIAAFHESANQTLGVCLENMMKSY"
     gene            complement(2879..>3203)
                     /locus_tag="VKL21_01175"
     CDS             complement(2879..>3203)
                     /locus_tag="VKL21_01175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032291.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMB44412.1"
                     /translation="IPPAIHAGGLRYHGDSPIVSQLYADKLIDAVAYHQKEVFDAAIT
                     FARSEGIVPAPESSHAIKCAIDKALVCKKSGEKKTILFNLSGHGHFDMGAYDNYFSGN
                     MKDSS"
BASE COUNT          763 a          731 c          599 g         1110 t
ORIGIN      
        1 agctggagta tgatgctttc accttcaatg aaactgaacc tgaaacttgc attattggga
       61 agcctctttg tcctgtgccc tgtgagataa gtatattcca ttttgaggag attatcgata
      121 agcttgtccc tcattttcat aagtctggcg ctttcatctg tcatttcttc ccgggctatc
      181 agggacgctt tgcccatccc gactatgccg ggaatatttt cagaaccgct cctcatcccg
      241 aattcctgcc ctcctcccag catgaaagga attatcctca caccctgttt gatatagagc
      301 gcgccaacac cttttggacc tcccatatca tttgaagaaa ttgtcaagaa gtcaatatgt
      361 tccttctcca catcaacaga aactttactg catgcagcgg tcccgtccac atggaacatt
      421 attttcctct ctgaggcgat ttcaccaatt tcccttatcg gctggatggt tccaatttca
      481 ccgctggcat acagtactga aataaggatc gtcttatctg ttatggcatt ctttacctgt
      541 ccgggatcca gaagcccgtc cttatcaaca ggtatataag tgacctcaaa tccgtttttt
      601 tgcagatctt ttaaagcatt catcacggac atgtgctcta tcgaagttgt gattatatga
      661 tttcctttat ctttatacct catcgctgcg cctcgaagag caagattgtt ggattccgtt
      721 gcgcacgaag tgaaaatgat gcttttggga ttgggcgcgc ctatcaactt tgcaacgcta
      781 gtgcgtgcat tttcaagctc cctttttgct tttcgtccct gcgaatgaag cgatgaggga
      841 ttcccgatat cgttttcaaa aaacggtatc atggcttcaa gcacacggac atccactggc
      901 gaagccgacc cgtgatccat gtaaacctgt ttcatattgt tcctttgatc tttatgttct
      961 tttttaaatc cgcctgtctc tgccctccgg cccttcacac tccggtatat agcaggtcgc
     1021 atctatgaag gtacatgctt ccttgcagct ggggcaagtt tccggcggtg ttgcttttcc
     1081 ttcgaactgg tatccgcaca ttgaacattt ccactggatt ttcataatta cctcctaaaa
     1141 ccacatagta ccgtcagctt caagcacaag gtcgttaaga gtggcagctc ccacaatctc
     1201 agcttctttt ataaaatcgc cccttgtaag attgatcagt tgggtgctct gttcacagac
     1261 cataagtttt acaccagctt ttgctgtctg atccatcact tccttaagag ttgggaattt
     1321 tcctatcttg atcttttcag cattccctgt cttcactacc gttattccca tgccaaggaa
     1381 atagattatc gcatctatac ccatggcttt tgcagtttgg gcaagaataa aaggtgaata
     1441 aaggcgttca ggtgtatcga ttccgcttgt ctggacatat aataggtttg tcattgtttc
     1501 tcccattttg atatatgatt aataacagat atacactttt ccttttataa ctgtcaactg
     1561 gaattaacga atggagattt caaaagttaa tcgttgaagt agggagccac cggtcttgtc
     1621 tgacagtgaa ttcacgaaat cctgaccctg ctatctatca ccgctacata ctcccgtaat
     1681 tttacattat ttgattcaat ttcctgttta agtgattcaa gctggtttct tgaagtatca
     1741 agttcttttt cggttattat tagcttttcc tgcaggtctg atagcatgtc tttcaggttt
     1801 ttttcttcct taattatttt catattggat atttcatctt tcttcaattc aatatcatgc
     1861 agaatatcat gatattcttt ccttgattcg cccagggagg atgcagcaaa ccgtatctgt
     1921 ggaaggaatt tctcaggttt tggaagattt aatacgccgc tttcaacgat tttctggaat
     1981 tcaacaaaga attccggagg aacatcggtc gggcatgata aggataaatt taacctctcc
     2041 ttatcctccg gtttcaaagc atgtctgcca ctttcgttca attgcttaag tctgttcagg
     2101 gctttattca atggcgatat gatgctcttg atttctgaga ccttattttc tgcttttgtt
     2161 tcaagcaaaa ccagatcatt tcttgatatt gtgtattgct tccaggattc gctatcgagc
     2221 aaaaggttca gatcattttg tttttcttca atttcttttt tcaaggaaac gctttttcct
     2281 tcgttttctt ttattgattt ttcaatcgaa tcaagatcca gcgtgttctt tctaatggcc
     2341 aggatcattt ccctggaatt ttccagggca tcaagcactt gctttttcga atttatgggt
     2401 tcgattattt cattaaggag ccttcccagg gaattgacct ccgatattac attttttgac
     2461 tcctcaaaat atacaagttt tgtatactag taactcttca tcatgttctc aagacagact
     2521 cccagggtct ggttcgcgct ttcatgaaag gccgctatcg cctttatatc tttatctttg
     2581 ggaatggtta agttttcaag aagcatcctc acctgttttg ccatattatc cctgttgctt
     2641 gaggccacct taactatctt aagatgatat cttccttccg gttgtgcttt ttcaagccgg
     2701 gatgtgcttt ccttgatttt cctgagtgtt ttttcgattt caggataaag agaagatgca
     2761 tccttttcaa gtttttctga aatgtttcgt gacctggatt caagccattc aggaagcata
     2821 tcctgagtta ttgatccgga tgatttttcc ttttttccaa aaagcttttt gatcaggttc
     2881 aacttgagtc cttcatattc ccgctaaaat aattatcata cgctcccata tcaaaatgcc
     2941 cgtgaccgct caggttgaat aagatggtct tcttttcgcc gctcttttta caaacaagtg
     3001 ctttatctat cgcgcatttt atggcatgcg atgattccgg cgcgggaaca atgccttcac
     3061 tgcgtgcgaa cgtaattgcc gcatcaaata cttccttctg gtgatatgca acagcgtcta
     3121 ttagtttatc tgcatataat tggctgacta tgggcgaatc accatgatat cgaagtcctc
     3181 ctgcatggat cgccggaggt atg
//
LOCUS       DATGBN010000057         8386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_81693, whole genome shotgun sequence.
ACCESSION   DATGBN010000057 DATGBN010000000
VERSION     DATGBN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81693"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /gene="eno"
                     /locus_tag="VKL21_01180"
     CDS             complement(<1..525)
                     /gene="eno"
                     /locus_tag="VKL21_01180"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499724.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMB44413.1"
                     /translation="MFTIEHIHAREILDSRGNPTVEVDVFTECGFGRASVPSGASTGT
                     NEALELRDKDKRYLGKGVQKAINNVNTEIAEALLGMDVRDQREIDAIMLELDGTENKS
                     KLGANAILGVSLGVARAAADSMDISLYRYLGGTNAFTLPIPTMNVINGGKHAGNELAI
                     QEFMLQPGGAKTFSE"
     gene            649..1650
                     /locus_tag="VKL21_01185"
     CDS             649..1650
                     /locus_tag="VKL21_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB44414.1"
                     /translation="MKTIKPPININIIIIIISTIIFLVAYAIVTIIPIEICSGNDCPN
                     QTINPGIIPTPDPGYGSTIAYLGDSQPASSSDAKELTKDLNKVIPLSPMGKVDAIFFM
                     GDMTKLSLTLQAVETSDVKNVPVYFVIGNHEAGSSSETALIQGRYSTSKFPLNPGPAG
                     TDKTTYSMNVGNIHVINMNEYWNGANDDSYLKYSSSDGGYVPDALYNWMSADLSRTSD
                     WKIVVGHDPLYPESKHVGNSLDANKANRDKLQALFVSENVDLFLGGHTHNAGVQNIGG
                     VYHANTGVIGPGAGGSGDGVASISYTYTTANNLILTIKQENPTWSTPKTITTKTISK"
     gene            complement(1640..2905)
                     /locus_tag="VKL21_01190"
     CDS             complement(1640..2905)
                     /locus_tag="VKL21_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMB44415.1"
                     /translation="MADLVIKGGTVLTMNGSTIENGVVGVEKGVITFVGKEMKEKAQK
                     VINAKGCAVLPGLINAHTHLPMTLLRGYAADMPYREWTQRIRKAETKLTPGDVMAGAC
                     LGALEMIRSGTTSFADMYIYMDEVAKAVEGIGIRAALGYGMIEGLNEDPEVKLKNREE
                     FVRKWDGAAHGRITTMYAPHSAGSCSKEFLIKVRELAQKTSSRIHIHVLETEDELNLM
                     KKRYGMCSINLLDNINMLGPNVLAAHCIWLSDDDIGILKVKDVNVVHCPSSNMALGAG
                     VARVPEMLEKEINVALGTDGAASAGSLDMWDEMRVASLLHKSKDPRTMPALKVLEMAT
                     VNGARALGINAGMLKAGCLADIIIVDLKKPKFISSDLIYSLVNGASGCDVRTTIVNGK
                     VLMEDHKIIGFDEEKIIEDAIKARSGIIC"
     gene            2989..4161
                     /locus_tag="VKL21_01195"
     CDS             2989..4161
                     /locus_tag="VKL21_01195"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HMB44416.1"
                     /translation="MKKVVLAYSGGLDTSVCIPLLKEHYGCDYAITVTVDVGQPAKDI
                     EQAEKKAEKISQKHYTIDAKDEFVKDYIFPLIKANGSYEGYVLGTSIARPLIAKKVVE
                     IAAKEKAQALAHGCTGKGNDQLRFEIVFRSTSLEVLAPMRDMNLTREWEMEYAQKHGI
                     PVTASKSKPWSVDENIWSRSIEGGRLEEPDFIPPEEIFEWTVSPEKAPAARVIEIGFE
                     NGVPVSLEGERSDGVPLIRKLNIIGGSHGVGRTDMIEDRVLGIKARENYEHPAATILL
                     MAHKDLEQLVLTRDELRFKVIVDETWSELAYKGLVDEPLFSDLNAFIDKTQERVTGNV
                     KIKLYKGSARVIARISPYALYSADISSFDSRTIDQKDAEGCCKYHGFQARLFKKLG"
     gene            complement(4145..5287)
                     /locus_tag="VKL21_01200"
     CDS             complement(4145..5287)
                     /locus_tag="VKL21_01200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMB44417.1"
                     /translation="MNFDYIIVGAGVIGSSTAYYLKKALPNSNILLLDRNQTAGAGNT
                     ARSAALYRNIFSSRTNRMLAASSIQYYLELGDKIKLDPIGYLWLFSKEQWNNYQRIVK
                     QLDLEKDEIEVLDRAAITSILKINYQAKAPFSDVHAGIYCHLCGSLSAMALARHYATE
                     FRSLGGEIGFDTDIISLNLAGKNSCYAPWDDVYINGITDQRGNIYNATRFIIATGAWT
                     HELLAPIGIASGILPKKRQIFVLKIGEPEQLIAHPEKTPAIILPAGGVYIKPILDKNI
                     LIAGCADDLGQPFMMSNPEPDPRYFHNAIEPVLVHYFPLLAGYELSHKWAGYYDYHWP
                     DMTPVIESVANITWVGGTSGSGIMKADMIKRRIETGTHSKFFNPAS"
     gene            complement(5395..5796)
                     /locus_tag="VKL21_01205"
     CDS             complement(5395..5796)
                     /locus_tag="VKL21_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HMB44418.1"
                     /translation="MEIFQIDENLYQSSMIDDVERAKMFDVCIDLAGGIDPDARDFKI
                     YLKWTIEDAGLPDLDVLKLVASFGYDLAYEKNMKVLVHCEQGINRASLLNGVILWMKG
                     MSGQEIVNYIRSKRPGALFNPNFLNYLVNLK"
     gene            complement(5847..5920)
                     /locus_tag="VKL21_01210"
     tRNA            complement(5847..5920)
                     /locus_tag="VKL21_01210"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5884..5886),aa:Val,seq:cac)
     gene            6047..6838
                     /locus_tag="VKL21_01215"
     CDS             6047..6838
                     /locus_tag="VKL21_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44419.1"
                     /translation="MQIKRFKKWHLTFKVLPVVLLIIILKLLAHQFNLEFISLNPLFT
                     GIVTANIFLLGFLIAGVLSDYKESEKLPGDMATSIETIIDECLIIHKNKKAKQALECL
                     GYTLDFTNSIINWFHKKERTRALMDKLSNFNDFFLAFESLTQANFIARLKQEQSAIRR
                     ILIRIHTIRETSFVSSGYAIAEAIAIILAIGLIFVKIDPFYEALFFVGAITFLQIYMI
                     LLIKDLDNPFEYSGGGPDEISLKPIEDLKLRIEEKIGATMIENAH"
     gene            6920..7025
                     /locus_tag="VKL21_01220"
     tRNA            join(6920..6956,6991..7025)
                     /locus_tag="VKL21_01220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6953..6955,aa:Gly,seq:ccc)
     gene            complement(7411..>8386)
                     /locus_tag="VKL21_01225"
     CDS             complement(7411..>8386)
                     /locus_tag="VKL21_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HMB44420.1"
                     /translation="VVWIKGDDIAEEIARYAQSHNVTKIVMGKPRRFGLFPSLARKIM
                     VRTNDIDVFLFAGKSEQKIPKKKIGMISPLNFVISAVAVIIGSILGFLFRDILGQINL
                     LFLMLLPVVLIAIFKGRGPSIFAAVLSVLIFDFLFIPPYFTFAVSDIRYFLSYLMFIA
                     FAFVISNLASDLKFKVQQLQQSESRNTTLYDLSRDLVTSQNIDQVLHLMIHHTRQILP
                     CEMAIFLPEKGQVSVRASTDRFQINQKELGIATWVWNNGEPAGVGTDTLPEAWAYYLP
                     MKTADASNGVVGFHFDNPGQILTPENKIVIDTIARLGALAIERIGGKE"
BASE COUNT         2460 a         1843 c         1670 g         2413 t
ORIGIN      
        1 ttcggaaaaa gtctttgcgc cgcctggctg aagcatgaat tcctgtatcg caagctcatt
       61 tcccgcatgt tttccaccgt taatgacgtt catcgtggga atcggaagtg tgaaggcatt
      121 cgttccccca aggtagcggt aaagcgatat atccatggaa tccgccgccg cacgagccac
      181 accaagtgaa acgccaagaa tggcattggc gccgagcttt gacttgtttt ccgtcccatc
      241 aagttccagc attattgcat ctatttcgcg ttgatcgcgt acatccatgc ccaggagtgc
      301 ttccgctatt tcagtattaa cattgtttat ggctttctgt actcctttcc ccaggtatct
      361 cttatcctta tcccttagct caagcgcttc gttcgtccct gtggacgcac ctgaaggcac
      421 gcttgcgcgc ccgaatccgc attctgtaaa gacatcgact tcaacggttg gatttccccg
      481 cgaatctaag atttctcttg catggatatg ctcgatcgta aacaatgaat atcacctttt
      541 tgactcaata gttgaagata tacttaattc taagcgaact accgcctaca agttggtggg
      601 tttctctggc gtacctttaa tatcatattc ggtaaaatag ttaaatttat gaaaacaata
      661 aaacctccaa ttaatataaa tattattata attattatta gtacaataat atttttagtt
      721 gcttatgcta ttgtcacaat aattccaatt gaaatttgtt caggtaatga ttgtccaaat
      781 caaacaataa atccagggat aattccaaca ccagatccag ggtatggttc aacaatagca
      841 tatcttggcg attcgcaacc tgccagttct tctgatgcaa aagaattaac caaagacctt
      901 aataaggtaa taccactatc gccaatgggt aaagtagatg ccatattttt tatgggagat
      961 atgaccaaat tatcactaac gctacaggct gttgagacat cagatgtaaa aaatgtacct
     1021 gtatattttg taataggaaa tcacgaagcc ggctcaagca gtgaaacggc tttaattcaa
     1081 ggaagatatt caacttcaaa atttccgcta aatcccggac ctgcaggaac agataaaacc
     1141 acatattcaa tgaatgtcgg aaatattcat gttattaaca tgaatgaata ttggaatggc
     1201 gcaaatgatg attcatattt gaaatattcc tcatctgatg gaggttatgt tccggacgcc
     1261 ttatataatt ggatgagtgc tgatttgtcc agaacatcgg attggaaaat agtagttggt
     1321 catgatccat tgtatcctga gtcaaaacac gtaggtaatt cgttagatgc aaacaaagcc
     1381 aacagggata aacttcaggc tttatttgta tccgaaaatg ttgacctttt cctcggtggg
     1441 catactcata acgcaggtgt tcaaaacata ggtggggtat atcatgccaa tacgggtgta
     1501 atcggtccag gcgctggtgg ttcaggtgat ggggttgcat caatttcata tacttatact
     1561 actgcaaata atttaatatt gacaataaaa caagaaaatc caacatggag tacaccaaaa
     1621 acaataacca caaaaacaat tagcaaatga tgcctgatct ggcttttatt gcatcctcga
     1681 ttattttttc ctcatcaaac ccgattattt tgtgatcttc catcaatact tttccattta
     1741 ctattgttgt tctcacgtca catcccgatg ccccattcac aagcgagtaa ataagatcag
     1801 aagatatgaa tttcggcttt ttcaaatcca caataataat atccgccagg caccctgcct
     1861 taagcatccc tgcatttatc cccaatgccc gcgcgccatt caccgtggcc atttcaagca
     1921 cctttaacgc tggcatagtt cgcgggtctt tgctcttatg aagcaatgaa gccaccctca
     1981 tttcatccca catatcaaga ctccccgccg acgcggcgcc gtcagtaccc agcgccacat
     2041 ttatctcctt ttcaagcatt tcaggtactc gtgcaacccc ggcaccaagc gccatattgc
     2101 ttgaagggca atgaacaaca tttacatctt tgaccttcaa tattccaatg tcgtcatcag
     2161 ataaccagat gcaatgtgca gcgagcacat tcggaccaag catgttaata ttgtccaaca
     2221 ggtttattga gcacatcccg taacgttttt tcatcaaatt aagttcatct tccgtttcaa
     2281 gcacatggat atgaattctt gagctggttt tttgggccag ttccctgact ttgatcaaaa
     2341 attcttttga acaggatcct gccgaatgag gcgcatacat cgttgtgatc ctgccgtggg
     2401 ctgcgccatc ccattttctt acaaactctt ccctgttctt aagtttaacc tccggatctt
     2461 cattgagccc ttctatcatg ccgtatccca gcgctgctct aatgcctatt ccttctacgg
     2521 cctttgccac ctcatccata taaatataca tatcagcaaa cgatgttgtc ccggatctta
     2581 tcatctcaag cgctccaagg cacgcacctg ccataacatc tcctggagtg agtttggtct
     2641 cagctttccg tatcctttgg gtccattccc tgtaaggcat gtctgcggcg tatccccgca
     2701 aaagcgtcat aggaagatgc gtgtgggcat tgataaggcc gggcaatact gcgcatcctt
     2761 ttgcgtttat gaccttttgc gctttttctt tcatctcctt ccccacaaat gttattacac
     2821 ccttttcaac gcccactacg ccattttcaa tagttgaacc attcattgtg agcactgtgc
     2881 cccctttgat tacaaggtct gccataaaat gtactaatat cggctatcat aaaataagtt
     2941 ttccagatgc aaaaactttt aatcttctca cgctctaaag cttaaaccat gaagaaagta
     3001 gtacttgctt attcaggcgg gcttgataca tccgtatgca tccccctttt aaaagaacat
     3061 tatggttgcg attatgcgat tacagttacg gttgatgtag gacagccggc aaaagatata
     3121 gagcaggccg agaaaaaagc cgaaaagatc agccagaaac attatacgat cgatgcaaaa
     3181 gatgaattcg taaaagatta catttttcct ctcatcaaag cgaacggaag ttatgaagga
     3241 tacgtccttg gcacatcgat cgcacgcccg cttatagcga aaaaggtcgt agagatagcg
     3301 gcaaaagaaa aagctcaggc tcttgctcat ggctgcaccg ggaaagggaa cgaccagtta
     3361 agatttgaaa tagtgttcag gagcacttcc cttgaggttt tagctcctat gagggatatg
     3421 aaccttacca gggaatggga aatggaatat gcgcaaaaac atgggatacc tgtaacagca
     3481 agcaagtcaa agccatggag cgtggatgag aatatatgga gcaggagcat cgaaggcggg
     3541 cggcttgaag agcccgattt tattcctcct gaggagatat ttgaatggac cgtctcgccc
     3601 gaaaaagcac ctgccgcccg ggtcatagag attggttttg agaatggcgt tcccgtatcg
     3661 ctcgaagggg aaagatcaga tggcgttccc ttgatcagga agcttaatat aattgggggc
     3721 tctcacggcg tggggcgaac ggatatgatc gaagacaggg tccttggaat taaggcgcgt
     3781 gaaaactatg aacatcccgc agccacgata ttactgatgg cgcataaaga ccttgaacaa
     3841 cttgttttga ccagggatga attgcgattc aaggtaattg ttgatgaaac gtggagcgaa
     3901 cttgcttaca aaggtcttgt ggatgagcca ctgttttcgg atctgaacgc ttttatcgat
     3961 aaaacacagg aaagagtgac cgggaatgtc aaaataaaac tatataaagg aagtgccagg
     4021 gtcattgcta gaatttcacc atatgcgttg tattctgctg atatatcctc atttgacagc
     4081 agaaccatag atcagaagga tgcggaagga tgctgcaaat atcacggctt ccaggcacgg
     4141 ttgttcaaga agctgggttg aaaaattttg aatgggttcc cgtttcaatc cttctcttta
     4201 tcatatcggc tttcattatg ccgcttcccg aagtcccccc cacccaggta atattggcaa
     4261 cggattcgat aaccggggtc atgtcaggcc agtgataatc gtaatatcct gcccatttgt
     4321 gggataactc atatcctgca agcagcggga aatagtgaac cagaacaggc tcgatggcat
     4381 tatgaaaata ccggggatct ggttcaggat ttgacatcat gaagggctga ccgagatcat
     4441 cggcgcatcc tgcaataaga atattcttat ctaatattgg ttttatataa accccgcccg
     4501 ccggaaggat gattgccggt gttttttccg ggtgcgcaat gagctgttcg ggctccccta
     4561 ttttcagtac aaagatctgt cgctttttgg gaagtatacc tgaagcgata ccgataggtg
     4621 caagtagctc atgtgtccag gctccggtcg ctatgataaa ccgggttgca ttatatatat
     4681 tgcctctttg gtctgtgatc ccattgatat aaacatcatc ccagggagca taacagctat
     4741 tctttcctgc caggtttaag gatatgatgt ctgtatcaaa ccctatctct ccaccgagag
     4801 atctgaactc cgtagcataa tgccgggcaa gtgccattgc agagagggaa ccgcacagat
     4861 gacagtaaat tcccgcatgg acatctgaga agggagcttt tgcctgataa tttattttta
     4921 aaatgcttgt tattgctgcc ctgtcaagga cttcaatttc atctttctca aggtcaagct
     4981 gctttacgat cctttgatag ttattccatt gctctttcga aaacagccat agataaccta
     5041 tcgggtcaag cttgatctta tctccaagct ccaggtaata ttgtatagaa cttgcggcca
     5101 gcatccggtt tgttcgtgat gaaaaaatgt ttctatagag cgcggcgctt cttgcagtat
     5161 tccctgcacc tgcagtttga ttcctgtcaa ggagaagtat attgctgttg ggaagcgctt
     5221 tttttaagta ataggcagtc gagcttccta tgacgccagc gccgacgatg atgtagtcaa
     5281 agttcatgaa cctgcggatg gtgcaaatac cattcatgta gtatttggca ttccaattaa
     5341 tataggtatt ccaataattc agccaccaag tggagcacaa aaagaataat attcttactt
     5401 caaatttacc agataattta aaaaattcgg attgaataaa gccccgggtc ttttgctcct
     5461 gatataattg acgatctcct gaccactcat tcccttcatc cacaagatta cgccgtttag
     5521 caaacttgcc cgattaatcc cctgttcgca atgaaccaga accttcatat tcttttcata
     5581 ggccagatcg tagccaaaag aggcaaccag ctttaaaaca tctaaatccg gcaatcctgc
     5641 gtcttctatt gtccatttca aataaatttt gaaatcacgc gcatccggat ctatgccgcc
     5701 cgccagatcg atgcagacat caaacatctt agccctttcc acatcgtcaa tcatggaact
     5761 ctgatataga ttttcgtcaa tttgaaatat ttccatacca aaaataaatg accccggcaa
     5821 atttatacta ttctttcttt tttgagtggg cccgctgaga ttcgaactca ggacctccgc
     5881 tgtgtgagag cgacgtcata accggctaga ccacgagccc ttagttgcct gataagtttg
     5941 tattgctaat aagtcttttt atgatttttt ttccaattta ttccgagagc caaatcaaaa
     6001 acataatatc caacccccat cattcagaaa taaaccggag aaaatcatgc aaattaaacg
     6061 atttaaaaaa tggcatttaa catttaaagt tcttcctgtc gtattattaa ttatcatcct
     6121 gaaacttctt gcccatcaat tcaaccttga gttcatttcc ttaaatccgc tatttaccgg
     6181 cattgttact gccaatatat tccttcttgg atttcttatc gcaggtgttt tatctgatta
     6241 taaagagagt gaaaaattac cgggagatat ggcaaccagc attgaaacaa ttattgatga
     6301 atgtttgatc atccacaaga acaagaaagc aaaacaagct cttgaatgtt tggggtatac
     6361 ccttgatttt acaaactcca ttatcaactg gttccacaaa aaagaacgta caagagcctt
     6421 aatggataaa ctttctaact tcaatgattt tttccttgcc tttgagtctt taacacaggc
     6481 gaattttatc gccaggttga aacaagaaca gagcgctatc cggcgtattt tgatacgaat
     6541 tcataccatc cgtgaaactt cttttgtttc aagcgggtat gcaattgcag aagcaattgc
     6601 cataattctt gccatagggc taatatttgt caagatagac cctttttatg aagccctctt
     6661 ttttgtgggt gccatcacgt tcttacaaat atatatgatc ctcttgatca aagatcttga
     6721 caatccattc gaatattctg gcgggggacc ggacgagatc tctttaaaac ccattgaaga
     6781 tctgaaactc aggatcgaag aaaagatcgg ggcaacaatg attgaaaatg cccattgata
     6841 ttgagtgtac cacaagtacc aaaaaagtta aataaagaat aactatctat atatcgcttg
     6901 aatcattatt gagcatgacg cggttgtggt ctagtggcta tgacaggggc ttcccaacaa
     6961 tttttgcagg tatagcaaag atcaggaaac agcctctaac ccgggttcga atcccggcag
     7021 ccgcacctaa ttatatgggt tatatgcagg atgacgttta ttggagaaca tttaaatata
     7081 tcctttgaga atgtataaat gattttattt gggggaaata tgaacaaaaa tatacttgtt
     7141 acaataggat taataattgc cacattaggt tttattatca gtgttgctgg tagttttaat
     7201 gtccgagacg ccgtcagcaa tgccattgtt attcagaatt ttatacttta ttctctatgt
     7261 ttaatcatcg ctggcgccag aagggaaaca taactataca gcaattcctt catctgcgaa
     7321 attaattcaa cataatgagg agtgcacgtt agattgaata tgttccaaga caatgcggca
     7381 atgaatggaa tcctactcaa agataccata ttattccttc cctccaatcc tttcaatcgc
     7441 cagagccccc aaccgggcaa tggtatcaat tacaatctta ttttcagggg taaggatctg
     7501 cccgggatta tcgaaatgaa atcctacaac accatttgag gcatctgcag ttttcatggg
     7561 aagatagtat gcccacgctt cggggagcgt atctgtaccc acaccagcag gttcaccatt
     7621 gttccagacc caggtcgcta tgccaagttc tttttggttt atctgaaacc tgtccgtaga
     7681 agcccttact gaaacctgtc ctttttcggg aagaaaaata gccatttcac agggtaaaat
     7741 ttgccttgta tgatggatca taagatgcaa aacctgatcg atgttctggg aagtgaccag
     7801 gtcccggctt aaatcatata gagttgtatt ccgggactcg ctttgctgaa gctgttgtac
     7861 tttaaattta agatcggatg caagattgct aattacaaag gcaaatgcaa taaacatgag
     7921 atacgataaa aaatatctga tatctgaaac ggcaaaagtg aaatatggtg gaataaacaa
     7981 aaaatcaaaa atgagaacgc ttaagacagc agcaaagata gacggccctc gccccttgaa
     8041 aatagcgatc aatacaacgg gtagaagcat taagaataat aaattaatct gaccaagaat
     8101 atcccgaaaa agaaaaccga gtatagaacc aataataaca gcaactgcgc ttatgacaaa
     8161 atttagggga ctgatcatac ctattttttt ctttggtatt ttttgttcgc tcttgcctgc
     8221 aaagaggaaa acatcaatat catttgtacg caccatgatc ttccgggcaa gagatgggaa
     8281 caatccgaag cgcctgggtt tgcccattac gatctttgtc acattatggc tctgtgcata
     8341 acgggctatc tcttcggcaa tatcatcccc tttgatccag accaca
//
LOCUS       DATGBN010000058         4298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_470228, whole genome shotgun sequence.
ACCESSION   DATGBN010000058 DATGBN010000000
VERSION     DATGBN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470228"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..696
                     /locus_tag="VKL21_01230"
     CDS             289..696
                     /locus_tag="VKL21_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44421.1"
                     /translation="MRKGDMLVLDKGFYAYYHYTDGLVKYGILPLIFPRKNFKLGKVL
                     DGVIPTLEFYLDKPNRIQEKLLFLKKLVSEFKEVIANWKSFKYVRSFIEDVFKIKKNA
                     LSLDKLHKYTSLFCQKNLLSCCPFDWHTCFHGF"
     gene            complement(954..1289)
                     /locus_tag="VKL21_01235"
     CDS             complement(954..1289)
                     /locus_tag="VKL21_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44422.1"
                     /translation="MNKKIIVVSVLITILVLLSGCVSSSKPVVLSKNAEGTLQGLNSV
                     TIVTADIENQGESGDVLVKVEVKAPDKKPMNQQQVTYIEKGMIKKVSFTFDTEFGDKI
                     YYSVTAEPN"
     gene            2718..2984
                     /locus_tag="VKL21_01240"
     CDS             2718..2984
                     /locus_tag="VKL21_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44423.1"
                     /translation="MLIQKILISPKVSEKIKCKHGLDPIVTKDILEGNRYIEKVGGNN
                     YMAIGLCSTGYITVFFEYEAGIAEIKTAYKASDWQIDLYKRKRS"
     gene            2987..3412
                     /locus_tag="VKL21_01245"
     CDS             2987..3412
                     /locus_tag="VKL21_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44424.1"
                     /translation="MKSKKKEIDYENIQPEELTGEEADFEVKLDAKIPVYCIDCHIKM
                     EPAKIDIKRGNIMMMNVNAYKCPKCGKELLDVETSSRIEREFALRHTEEIRGYEVKIS
                     SDGRNYLIRFPKELSKMLATKKFAKILPVDTDEFVVKVV"
     gene            3603..3755
                     /locus_tag="VKL21_01250"
     CDS             3603..3755
                     /locus_tag="VKL21_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44425.1"
                     /translation="MKRWKRQKKQRNKRTERTPAGCDMGQTDEGALTGAVFGRRDFKG
                     QHGRIN"
     gene            complement(3752..3997)
                     /locus_tag="VKL21_01255"
     CDS             complement(3752..3997)
                     /locus_tag="VKL21_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB44426.1"
                     /translation="MEKTLRIISTTSIIDLDKEIAILGGKIYSHLVREGKEIELNDCL
                     IVATALSYGIKKVVTRNIDHFNKIKELKAVTPEDLDF"
     gene            complement(4131..>4298)
                     /locus_tag="VKL21_01260"
     CDS             complement(4131..>4298)
                     /locus_tag="VKL21_01260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034342.1"
                     /note="catalyzes the ATP-dependent breakage, passage, and
                     rejoining of double-stranded DNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HMB44427.1"
                     /translation="SELTDPRFDTPYWKEQINLMLKIGKKAEQQAFAGKGLDYVTDKY
                     LPERLTEMGVI"
BASE COUNT         1354 a          738 c          905 g         1301 t
ORIGIN      
        1 attcactaca aagggaatct acctcttcct attttttcct gttatatgtt ccatagatta
       61 gctgattctt aaacgactga tagcaccatt atgtggaatg aattagggca ataaggatat
      121 tcttgggaat ccgaatccat ccaataaccc tactttgaca ggtaaataac aatattgtcc
      181 tcaacatgtt tgttattgat gaaataccaa actaaactaa ggacaatatt attatttatc
      241 tgtcaaactt gggataatat cgtaaatgag ttgttgagaa ggagagttat gagaaaaggt
      301 gatatgctgg tgcttgataa agggttttat gcatattatc actacaccga tggattagtg
      361 aaatatggta tattgcctct gatttttcca aggaagaatt ttaaactggg aaaagttttg
      421 gatggcgtta taccaacact tgaattttat ctggataagc caaatcgcat tcaagagaag
      481 ctattgtttt tgaagaaact tgttagtgaa tttaaagagg tgatagcaaa ttggaaatcc
      541 ttcaaatatg ttagaagctt tattgaagat gttttcaaaa ttaagaaaaa tgcattgtct
      601 ttagacaaat tgcacaaata tacgagtttg ttctgtcaaa aaaacctgct ctcttgctgt
      661 ccttttgatt ggcatacttg cttccatggg ttttagagaa aaagtaacct tacagaagtt
      721 cgcagaatgg taagatttgg gagggggata aagagtatat aaacaacatc atttaagtgc
      781 tatttgtcta ccacccatgc tcatttcata gtttaaaggg gtgagagttg gggggaccag
      841 ggaaggagag agaatggatt aaaataatat attattatat attcataaat aatattagtt
      901 ttatagtaaa acagtaaagc tctggacctc accccaaagc ctacaacaga ttgttagttt
      961 ggttcagcgg taactgaata ataaatctta tctccaaatt ctgtatcaaa agtaaaactt
     1021 acttttttta tcattccttt ttcaatgtat gtcacttgtt gttggttcat gggttttttg
     1081 tctggagctt ttacttcaac ttttacaaga acatcaccag actctccttg attctctatg
     1141 tcagcggtta caattgtaac actatttaag ccttgcaatg ttccttcagc attttttgaa
     1201 agaacaacgg gctttgacga cgaaacacac cccgataata aaaccagtat tgttattaaa
     1261 acacttacaa caataatttt cttattcata tctataaccc catctaattt aagagtggta
     1321 gaactgtttt gagaggacac ttacccactc tgacaacttt cttccaagtg ttatttttat
     1381 ttaaagttaa cgccatgcac caccctcata ttctatggcg tcaagttagt tatgtaacat
     1441 tataaatata ttgtgataag aaaatgtgtt tatagtttcc aatacgataa aaaacatata
     1501 cgagcggacg gggagtgggt ttgcagtagc tccatgcaaa aataattcgg gctgtcaggc
     1561 gccgccggga tgatgcgcat gggggggtag gtgactgttc aatggttggt gatgttaaga
     1621 ttttatattc gatagagata gtattagtaa tattatggat tcaaccgtct gcatacctaa
     1681 aagtaaatat gatgaattat tacaaaaagt aaaccttctt aataccataa ttgactcaga
     1741 gagtttgact ttggaggaac ttgaaaggtt aaaacgagcg aggatgggtc ccagtgtaac
     1801 cgaagaggaa tttctaaaac agcatccaga gctcaaataa aaattaataa tgtacgaact
     1861 tataataaaa gaacagttcg ataagtcttt ctcgaaaata aaggatttta aaacaaaaaa
     1921 gcaaatctgg accaagatac tggaacttaa aacaatggcc ccgataggca aaaaattagt
     1981 gggaaatcca tattggtcaa tacatattgg aaaatttagg gtgatttata tttttcacaa
     2041 acacagcaat gagatcgaaa ttgttgatct attgagcaga aaacacaatt acagggagat
     2101 ttaatcatac ttttttgtat ttttctggaa attattaagc tgctaataaa ttgagttagt
     2161 aaagattcgt ttcaagaaat tgttatatct attcttttca cgacccgcgc ccggcatcgg
     2221 gtgaagggag ggcgggggtt tgcagcgcct ttatattaaa ataattcagg ctgtcaggcg
     2281 ccgccgggac gatgcacatg gggggcatgt gactgtgaaa tgagtgcgtt ttcttctggt
     2341 ccgatgtttc aggttgtgga ctccgcaatt ggaaaggtgg caacggcaga aacaaaacaa
     2401 cgaacaatac gaactgaacg aactccgatg gtatgagttc gtttcagttc gtccgagtta
     2461 gttgttcttt ttgtctgttt ttttggcgat gtgaaaagat gtatgcagct tctctatttt
     2521 cataaaagac ggcgctgtgg gcaggatgcg gtgtggggca gctgtttgtg tggactttgg
     2581 caggcgctgg gttcatgtta agaatatttc atagtaagcc gtcacaacaa gaaggcaatt
     2641 caaaagtatg gaaaatttta atagattgtt cttcgaaatg tttatctatt catacgaaaa
     2701 tcatacgtat gatatgaatg ctgatacaga aaatacttat ttcacccaaa gtttcggaga
     2761 aaatcaaatg caaacatggc cttgacccaa tcgtaacaaa ggatattctt gagggtaatc
     2821 gctacataga aaaagtaggt ggaaataact acatggctat aggattatgt tcaactggct
     2881 atattacagt attttttgaa tatgaagctg gaatcgctga gattaaaacc gcatacaagg
     2941 catcagattg gcagattgat ttatataaga ggaagagaag ttgattatga aatcgaagaa
     3001 gaaagaaatt gattatgaaa atattcaacc tgaagagcta acaggtgagg aagcagattt
     3061 tgaagttaaa ctcgatgcca aaattccggt atattgcatt gactgtcata taaaaatgga
     3121 acctgcaaaa atcgacatca aaagaggcaa tattatgatg atgaatgtga atgcatacaa
     3181 atgtccgaaa tgcggcaaag agcttttaga tgttgagact tcctccagga tagagcggga
     3241 gtttgcactc aggcacaccg aggagatcag gggctatgaa gtcaaaatat cttcggacgg
     3301 gaggaactat ctgatacgat tcccgaaaga gctttccaag atgcttgcaa ccaagaaatt
     3361 cgcaaaaatt ctgcctgtgg atactgatga atttgtggta aaagttgtgt gaaataaaac
     3421 tttcattttc cgctgttttc gatccgcgac gagcatccgg tgatgggggg gcgtgggttt
     3481 agggcgcctc tatgttccgg ctgtcaggcg ccgccgggat aatgcgcagg gggcagccga
     3541 atgggcgtgt tttgtccggc gctgtgttcg agcgcagaga tttcgggata tagactccgt
     3601 aattgaaaag atggaaacgg caaaaaaaac aacgaaataa acgaactgaa cgaactccgg
     3661 caggatgcga catggggcag accgatgagg gcgctttgac cggtgctgtg ttcgggcgca
     3721 gagatttcaa ggggcagcac ggtcgcatta attaaaaatc caaatcttct ggagtgacag
     3781 cttttaactc tttgatttta ttgaaatgat ctatatttct tgtcacaacc tttttgattc
     3841 catatgatag tgcggttgca acaataaggc aatcatttaa ctctatttct tttccttctc
     3901 gaacgagatg cgaataaatc ttgcctccaa gtatcgctat ttctttatcg agatcaatta
     3961 tagatgttgt tgagattatt ctaagggtct tctccaaagc atctgtcttt ccggcactat
     4021 gctaatatag ccttcatctt cctcgctata gaaaatttct attgcatacc tgttaaaacg
     4081 cacattatta ggattccttt tcaattcatc gtaaacagct ctcttgtgga tcaaatcact
     4141 cccatctccg tcaacctctc cggcagatac ttatccgtaa cataatccag acccttcccc
     4201 gcaaaagcct gctgttcagc ctttttccct atctttagca tcaggtttat ctgctctttc
     4261 cagtatggcg tatcgaaacg ggggtctgta agctcgct
//
LOCUS       DATGBN010000059         6911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_276159, whole genome shotgun sequence.
ACCESSION   DATGBN010000059 DATGBN010000000
VERSION     DATGBN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276159"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VKL21_01265"
     CDS             complement(<1..967)
                     /locus_tag="VKL21_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HMB44428.1"
                     /translation="MPEYTLFLGCIIPARFPFMEKSTRLVLSRLGCILHDLEGATCCP
                     TKSIIKPIGDLAWYVTAARNLALAEKAGHDLLVPCNGCYSTLKSVEVEMRINPGIREE
                     VNSILASAGLEYRGMVGVKHLVEVLHDKIGIPKIKQHITKSFEGIRIASHHGCHMLRP
                     SSSIFFDDPNKPKKFDALIEALGAKSIDYETKMLCCGGNLNTSDEPDEATAMARMKLI
                     EVNKKADAIALTCPSCFMQYDSRQYMMQKAGEKLNVPILFYPELLGLALGFSTQELGM
                     EMHRIDAAEFLSKWDSKYKYLKTLKEVFDLGAIRKCYECGACVNDC"
     gene            1539..2384
                     /locus_tag="VKL21_01270"
     CDS             1539..2384
                     /locus_tag="VKL21_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMB44429.1"
                     /translation="MNSRAKFHNNPYISILKLKGSIVDISVVAVTCAILGRWDYRAAL
                     VILAGIMIHSGCDIINDIYDIEIDKICKPNGAIASGQISVKNAWIYMMLLFSTTLSLA
                     LVLSLILFACLLIGIIVGGAMYSHPLFRLKDKPGIAMADMALCFALESIGAWNIYSYF
                     NSDSLLVAIYVFVLIFSLTFMKDFKDVAGDVNSLPIKLGIRRAATVCSALTLLPLIPM
                     IYASMKYHYLWLAIAVYAILIAGCIKILMRDPVAEGKRLKDMMILALTAPNFIMLLTT
                     MALTL"
     gene            complement(2379..3035)
                     /locus_tag="VKL21_01275"
     CDS             complement(2379..3035)
                     /locus_tag="VKL21_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44430.1"
                     /translation="MNEIIPDRIKKIAQIISEVSHLDETDMFILLSLMQDSKTTNAEL
                     AKIMNFKDGNSVAYHTRIMQKEGIIDRYTIIPDWKRIGVATEFIILAEAENEEQLLEI
                     EKRHIIMADEYSSKQGDIVVTQTISGCVILQDIYHCFGDKTMSVIIGRATSDQDAAVY
                     CKNYIVGRYPNIKVSLLINKYKTISEFFIDKHAIEKLKEFFKIENTGDVLKELHALPL
                     "
     gene            complement(3698..5338)
                     /locus_tag="VKL21_01280"
     CDS             complement(3698..5338)
                     /locus_tag="VKL21_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HMB44431.1"
                     /translation="MIINRIYGEHLHRIAIGISLFAILLLVSIAGAVPYAYIANTGSD
                     NVSVSGISFTGPTPTNGTILAQNFVYINTTVSNASTAFIDWNRSLVGWWRFNSETGEN
                     STFFRDWSTWENNATCSGINCPASTSGKFGNALRFDGVNDYVDTGNAPGLNITGNITI
                     EAWVKPERLETAHIVKKATRDSTDGYELSLSESTGKAYFRVNQKTSHNTYKLFSNSSY
                     PLDGNTWVHLVGVYNRDQVQIYFNGNLENSMSGPTNILSNTDNLKIGGPDLSRYFKGT
                     IDEVRIWDRALSPEEIKASYDAGIYRLYSNFTNLANGVYNYRTYAQNLQGNVNQTEKM
                     TLHVNLGLENTPISPVIKIMPLGDSITSSDSSYRNRLWDLLANNSINVDFVGSLSSGY
                     ETLPDKDHEGHKGWKIDKISARVEDWLSIYRPDIVLLHIGTNDISHNYNLETAPDRLG
                     TLIDKIAVKSPKTKVIVASIIPRKTDNNATIAYNNAIRGVVTNRQSQGKNVYFADIYN
                     EAGINTDTDLSNEVHPNIKGSNKMADVWFKQLKIVLSQ"
     gene            5811..>6911
                     /gene="dnaG"
                     /locus_tag="VKL21_01285"
     CDS             5811..>6911
                     /gene="dnaG"
                     /locus_tag="VKL21_01285"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HMB44432.1"
                     /translation="MQNSDTTKYIIHATIKADGVIERPDVVGAIFGQTEGLIGADLDL
                     RELQKSGRIGRLEVNIKSNMGKSAGTIDIPSSLDKVETAVLAASLETIDRIGPCISNI
                     SVVKIEDVRSSKRKQIVERAKQVLTAMFDEMLPESQEIIDEVRQSVRIEEMTTYGKDN
                     IPMGPNVLDSDAIIVVEGRADVLNLLRYGIKNAIAVGGTNVPQAVADLCKKKTVTAFT
                     DGDRGGELIIKELLQVADVDFIARAPDGKGVEELVQKEVVKSLRQKIPVEQAFDYYQV
                     GKKRRIAILAATKKKAGKGEPLVSEEVEIKFEPAEIEPVAEPERPRTRAEPRAENRFE
                     SRAEHREEPRYEKEVKESPFQGHITDLSGTLSA"
BASE COUNT         2133 a         1314 c         1347 g         2117 t
ORIGIN      
        1 ggcagtcatt gacacaggcg ccgcactcat aacatttacg gatagcgccc aggtcgaaca
       61 cttcttttaa tgtcttcagg tatttatatt ttgaatccca cttcgacagg aattccgctg
      121 catcgatcct gtgcatctcc attccaagtt cctgtgttga gaaacccaag gcaagtccta
      181 acaattcggg ataaaataga attggcacgt tcaatttttc gccggccttt tgcatcatat
      241 attggcgtga atcgtattgc atgaagcagg aagggcatgt aagcgcaatt gcatctgcct
      301 tcttgtttac ttcaatcaat ttcatacgcg ccatggctgt tgcttcgtca ggttcatcgg
      361 atgtgttgag attaccgccg cagcacagca tttttgtttc ataatcaata ctctttgccc
      421 ccagggcttc tattaaagca tcgaattttt tgggtttgtt ggggtcatca aagaatatgg
      481 atgaactggg tcggagcatg tggcagccgt gatgggatgc tatcctgata ccctcgaagg
      541 attttgttat gtgctgtttg atcttcggta ttccgatctt atcatgaagg acttcaacaa
      601 gatgtttgac ccctaccatt cccctgtatt ccaggccggc agaggcaaga attgagttca
      661 cttcctccct gatcccggga tttatacgca tttcgacttc cacggatttt aatgtgctgt
      721 aacaaccatt acacggaacc agaaggtcat gaccggcttt ttcagccagc gccagatttc
      781 ttgctgctgt tacgtaccag gccaggtctc cgatcggttt tatgatactt ttcgttgggc
      841 agcatgtggc cccttcaaga tcgtgaagaa tgcagccaag cctggataaa acaagcctgg
      901 ttgatttttc catgaaggga aaacgcgcag ggatgataca acctaaaaat aacgtgtatt
      961 cgggcatttt ttcctcttgt tggggaagct gaccatagct attatagtat ttaaatttaa
     1021 ctcagaaata ggaaggatac tggcgatata ttatgcaagt aacaaattta aataatgtgt
     1081 ttgcatctaa ctggccggat aataatttca ggagcgctcg attttttcgc gtgcccaaag
     1141 attgctcagc aaacctcctc gcatttgctt catcaagcac aagcattcga taaatgttaa
     1201 agagtttctt ttttttatat atttcttgcg ataatgggga aaacttaata aagcattagt
     1261 tttaatatat taaaagggtc ggcatataaa gtgtattttc agactttaac ccaagcctcc
     1321 atattttata tgccaactca tcttactcta tgaaatcatg agcatgtaca aatcacataa
     1381 tggttcttca attataaaag gattttctcc ttttcatcaa cttgatttga tttattataa
     1441 ttaaacttat cgattaattc actattctta ctgaaaacga aaaataaaaa aaagataaca
     1501 ataatatgaa ttttatatat aaaaaaaatt aatatagtat gaattcaagg gcgaaatttc
     1561 acaacaaccc atatatatct atccttaaat taaaaggaag tatagttgac atctctgtgg
     1621 tggcagtgac ttgcgctatt ttaggtaggt gggattacag agcagctctt gtaattcttg
     1681 caggaataat gattcacagc ggatgcgaca taataaacga tatctatgat atcgagatag
     1741 acaaaatatg caaaccaaat ggagccattg catccggtca gatatcagtc aaaaacgcgt
     1801 ggatatatat gatgttatta ttttcaacaa ctctcagtct tgcactcgtt ttaagcttaa
     1861 ttctattcgc atgtctttta attggaataa ttgttggcgg tgcaatgtat tcacacccat
     1921 tatttcggct caaagataaa cctggcatag caatggcaga tatggcattg tgtttcgctc
     1981 tggaatcaat cggcgcctgg aatatctatt cgtactttaa tagtgacagt ctcctggtcg
     2041 caatctatgt atttgtactg atattctctc taacattcat gaaggatttt aaggatgtgg
     2101 caggagatgt taattcattg ccgataaagc tgggaattcg cagggcagca actgtgtgca
     2161 gtgcactgac attgcttccg ctcattccca tgatatatgc gagcatgaaa tatcattatt
     2221 tatggctggc tattgcagtc tatgctatac ttattgcagg atgcataaaa atcctgatgc
     2281 gagatccggt agcagaagga aagcgactca aagacatgat gattctggca ttgactgcac
     2341 caaactttat tatgttattg accacaatgg cattgaccct ataaaggcaa agcatgcaat
     2401 tcttttaaaa catcaccggt gttttctatc ttgaaaaact ctttgagttt ttctatggca
     2461 tgtttatcga tgaagaattc acttattgtt ttgtatttat ttatgagtaa gcttactttg
     2521 atgttggggt acctgccaac aatataattc ttacaataaa cagcagcatc ctggtccgac
     2581 gttgcccggc caattattac cgacatcgtc ttatcgccaa aacagtgata gatatcctgc
     2641 aggatgacac agcctgagat tgtctgtgtt acaacaatat ccccttgctt cgacgaatat
     2701 tcatcagcca ttattatatg tcttttctca atctcaagta gctgttcttc attttcggct
     2761 tctgccagta tgataaattc agtcgccacc cctatacgct tccagtcggg aataatagta
     2821 tatcgatcaa ttattccttc tttctgcata atgcgtgtgt gataagctac agaattccca
     2881 tccttaaaat tcattatttt tgcaagctca gcattggtcg tcttgctgtc ttgcatcagg
     2941 ctcagcagaa taaacatgtc ggtttcatct aaatgcgata cttctgagat tatttgtgcg
     3001 atttttttaa ttctgtcagg tataatttcg ttcattatga tcaacgacaa tatatattcg
     3061 tttagctaaa atagatattg ccttaagaag tatttaactt ttgcattaga ataaaagacg
     3121 taccgcattc aaagcacctg atattgtcaa taagcgaaaa atatagcatc aatgtatcct
     3181 gttgtaaatt gggaaaaact taataaagga ttagtcataa catagaaaaa gggtcggcat
     3241 ataaggtata ttttcagact ttaacccaag cctctatcct ttatatgcca actcatctta
     3301 ccccatgaga tcatgagcat gcacgaatca tataatagtg ttcaattata aaaagctttt
     3361 cttcctttaa tcaacttagt ttcaggaaat atatttaaac ttgtcaattt ttgtataatt
     3421 ctaattagaa acgaaagaaa aaaaataaat aacaaaaaat tgaattttat atacaaaaag
     3481 aattcataca atatgaattc aaggacgaga tttcacaatg taaaaaaatt tagtataata
     3541 gctcaaagta gacaggtagt taaggtgtgt ctcaatgagg gaaatcatgg gaaaaacatc
     3601 aagaaaaaga tatacgcgtg atgttatatt tcgtaattgt tgattcaaat tcttatttct
     3661 tccatctaac atgatctctc ctctttgacg gttgtttcta ttgagataaa acaatcttca
     3721 attgtttaaa ccagacatca gccattttat ttgaaccctt gatattagga tgaacctcat
     3781 tcgatagatc agtatctgta ttaattcctg cttcattata gatatccgca aagtagacgt
     3841 tctttccctg gctttgcctg ttagttacta cacctcgaat tgcattatta tatgcgatag
     3901 ttgcattatt atcagtctta cgcggtataa tggaagctac gattactttt gttttaggtg
     3961 attttactgc aatcttatca ataagagttc ctaatctatc aggagctgtt tcaagattat
     4021 agttatgaga tatatcatta gttcctatat gcaataaaac tatatcgggc ctgtatattg
     4081 ataaccagtc ctctaccctg gcagaaattt tgtctatttt ccatcccttg tgtccctcat
     4141 ggtctttatc gggcagtgtt tcatatccgc ttgacaatga accaacaaaa tctacattaa
     4201 tactgttgtt tgctaaaaga tcccacagac gatttcgata actgctatct gacgaagtta
     4261 ttgagtcgcc cagtggcatg attttaataa ccggggatat gggagtgttt tccaaaccta
     4321 aattaacatg caaagtcatt ttttcagtct ggttcacatt cccctgcaag ttttgagcgt
     4381 aagttctgta attgtaaact ccgtttgcca ggtttgtgaa attactatac aatctgtaaa
     4441 ttccagcatc ataagaggct tttatttctt cagggcttaa tgcacgatcc catatgcgga
     4501 cttcatcaat agttcctttg aaatatctgg aaagatcagg accaccaatt tttaaattat
     4561 cagtattaga taaaatattt gtgggacctg acatgctatt ttcaagattg ccattgaaat
     4621 aaatttgtac ctggtccctg ttatatactc caactaaatg aacccaggta ttaccatcta
     4681 aaggatatga agaattgcta aaaagtttat aagtattgtg tgacgttttc tggttgactc
     4741 taaagtaagc tttaccggta gattcactca aagaaagttc atagccgtca gtagaatctc
     4801 gtgtagcctt tttcacaata tgtgctgttt ctaatctttc aggttttacc catgcttcaa
     4861 ttgtgatatt ccctgtaata ttaagacctg gtgcatttcc tgtatcaaca taatcattta
     4921 cgccatcaaa cctcagggca tttccaaact ttccagatgt tgaagcaggg caatttattc
     4981 ctgaacatgt ggcgttattt tcccaggtgc tccagtccct gaaaaatgtc gaattctcac
     5041 cagtttcact attgaacctc caccaaccaa ctaaagaacg attccaatct atgaaggctg
     5101 ttgaagcatt agaaactgtt gtgttgatat aaacaaaatt ttgagcaagg attgtaccat
     5161 ttgtcggagt ggggccggta aaggagatac ctgatacaga tacattgtca ctaccagtgt
     5221 tggcaatgta tgcatatggt accgcacctg cgatacttac cagcaataag attgcaaaca
     5281 gtgatattcc aatggctatt ctgtgtagat gttctccata tattctatta ataatcatat
     5341 ataccacctt ttaaagattt catggacaac gaataattgt tgcccgcttt gtgccaccat
     5401 cgcaaatatg gttatcctag cataatgctt tttcgatatt atccgcgagt cattttatga
     5461 taataatagg ggttatgaga tttttggttt atgaccgcaa agtttttaaa tagatatatc
     5521 caattatagc gtgagtttgc attgatggaa aaatcaaaca tccatgcgaa cattatccaa
     5581 cgaaaagtag ttagaattag atatttcata atcatatagg ggtcacaaag actcatcgtg
     5641 gcctgataga tcaatgttac tagtcattta cagtttgggt tataagataa agtcaaagag
     5701 tcctggacta atacagtact tgtttagttc aggataagta ctttcaaaga tagccaggtt
     5761 cagaccgatg aaatcttctt ctaacactgt tcaaagggag ggtacaatat atgcaaaatt
     5821 cggatacaac aaaatatatc attcatgcaa caataaaagc agacggagta atagaacgcc
     5881 cggatgttgt cggagcaata ttcggacaaa ctgaagggtt aatcggagcg gatcttgacc
     5941 tccgggaact acaaaagagt ggcaggatcg gaaggcttga ggtcaacata aagtcaaaca
     6001 tgggaaaatc agcaggaaca atagatattc catcaagtct tgacaaagtt gaaaccgcag
     6061 tcctcgcagc aagccttgaa accattgaca ggatcggccc ttgcatctcc aatatcagcg
     6121 ttgtgaaaat tgaagatgtg agaagttcga aaagaaaaca aatcgtagag agggcaaagc
     6181 aggtccttac ggccatgttc gatgaaatgc tcccggaaag ccaggaaatc atcgatgaag
     6241 taaggcagtc cgtcaggatc gaagaaatga ccacatacgg aaaagataat attcccatgg
     6301 gccctaatgt ccttgattct gatgccatta tcgttgttga gggacgggca gatgtcctga
     6361 accttcttcg ttacggcatc aagaacgcca tagctgttgg cgggacaaat gtgccgcagg
     6421 cagttgcaga cctttgcaag aagaagactg tcacggcctt cactgatggt gacaggggcg
     6481 gagaattgat aatcaaggaa ttactccagg tagcagatgt ggatttcatt gcacgcgccc
     6541 cggacggaaa aggtgtagag gaacttgtcc agaaagaggt tgtaaaatcg ttacggcaga
     6601 aaatacctgt tgagcaggca ttcgattatt accaggttgg taagaagaga agaatagcga
     6661 tacttgcagc gacaaaaaag aaggctggaa agggcgaacc tttggtttcg gaagaagtgg
     6721 aaataaagtt tgaacctgcg gaaattgagc cggttgcaga accggaaaga ccaaggacaa
     6781 gagcagaacc aagggcagaa aatagattcg aatccagggc cgaacacagg gaagaaccaa
     6841 gatatgaaaa agaggtaaag gaaagcccat tccagggaca tataacagat ttgagcggca
     6901 ccctcagtgc a
//
LOCUS       DATGBN010000060         6158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_276179, whole genome shotgun sequence.
ACCESSION   DATGBN010000060 DATGBN010000000
VERSION     DATGBN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276179"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1431
                     /locus_tag="VKL21_01290"
     CDS             139..1431
                     /locus_tag="VKL21_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMB44433.1"
                     /translation="MSNNYIFSKLKSGKTLINYAFLKISSQGFLFLLSLILAKVLSPD
                     RFGAYSLSLMIIYFFSALFMRSSQDPSIVLGSDELKQHSKINKIFTVRIILLIQAIIL
                     FYLITRVFFQPLSHFSSLDETGFIFLFFAYSGLCIRNFFEGTFLALNQRMAEAAYDAL
                     TNIISFVWLFVIYYFFTINIENILMMFFVSPLIASLFLTREIDLKKVLPIEFDLRILR
                     QMFDYTKWTIMGSVGLYLVNWVDIFVLRIFVSLDQIGVYNLGYQIFKGVLILISIARV
                     YYLPFISEHIKDEKKMKNYLKNKRWIMMAAGSIGICLIYIALPYLFSILYADTYQGSV
                     TVARILLVGAVFSLYQTFYSPIFDAMRRYEFTQLASILMVAINLALDYILVFLYGFLG
                     AAVATAITYAILSLIYEMYFRKNLKNIIFSSPQRPTIR"
     gene            1464..1835
                     /locus_tag="VKL21_01295"
     CDS             1464..1835
                     /locus_tag="VKL21_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMB44434.1"
                     /translation="MSGKNVTKVLLFLKNMVYESTSPNEILRFAKYYRKKGLDVVLVL
                     WGPMGVILGKKGKTGTPAYDDRVRECIEMGIKIKCCELASSMIGLEKDELIEGIELIH
                     SFEVADIMLEYCEEGQLVINL"
     gene            1875..3023
                     /locus_tag="VKL21_01300"
     CDS             1875..3023
                     /locus_tag="VKL21_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HMB44435.1"
                     /translation="MKLNLSDAQAFLMVSESEHNADMYYASGFLAYDPFVYLNSGTDL
                     ILISDMELGRARKESKVHDVIPTSGFNIMDKIRKTRDADAAYCEMITELIHSKNLNRI
                     AVPYNFPVQLADCLRGAGLEIIPIKSPLREMREIKNEREISAIEYAQRAGEKALAEGI
                     RAIKNTDVKDGVLMYENSPLKTQDIRAIIDRSLLSFNCEAPDIIIACGRGSSDPHWKG
                     EGELLADEPIVIDMVPRSKKDRYYSDMTRTVMRGTPSKELKDMYSAVLDSQICAIKKI
                     KAGVTGADIHNIVCDVLEERGYKTARCKNTEFTEGFIHSTGHGVGLDIHEGPNLGENG
                     KELKAGCVVTVEPGLYYKKIGGVRLEDVVVVTRSGCNNLTMFEKNLLL"
     gene            3023..3898
                     /gene="map"
                     /locus_tag="VKL21_01305"
     CDS             3023..3898
                     /gene="map"
                     /locus_tag="VKL21_01305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HMB44436.1"
                     /translation="MTETDETIEKYRQAGRILSEVRKLTAEKVTEGASLLMVAEFGEN
                     LIREKGAQPAFPVNISRNDEAAHSTPCLNDKSVFGKDMVKLDIGVHIDGYIADTAITV
                     DLSGNPELVKAAESALEEAINFIKAGVNTSDIGGVIEDAITSYGYKPIINLTGHGLAQ
                     YIQHAPPAIPNKRMPGGVILEEGDIIAIEPFATNGAGKIHDAGSAEIFHIVSDRPVRH
                     PSARKLLKEIEKYKTLPFAKRWLGERVDFAMLALSKANIIRPYPILKEIQGGLISQAE
                     HTIIVTADGCEVVTI"
     gene            3895..4692
                     /locus_tag="VKL21_01310"
     CDS             3895..4692
                     /locus_tag="VKL21_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44437.1"
                     /translation="MKISGIFISLILLFTATDSVAAENRWVNNITVNEYNIAWGYTET
                     FAGMDSIAYRINLDSKIGNNDSFINAWELLKADKDSREKFKNSMENEPDVRINNETSG
                     IEMIDVDSSLSQAIIGKTHIADTIENRYKVTYRLKESIFNDSSIWFLGEPESPITIIL
                     PQGIDVIGISGMNNETKNIDSNTEVSGYFSNSSNESAQITVNISKNASYNLTSPETNI
                     TEVVPAQNNENITEPASQVSEKIRKWSIIGVGIVFIVLIYVFRVRKN"
     gene            5215..5796
                     /locus_tag="VKL21_01315"
     CDS             5215..5796
                     /locus_tag="VKL21_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44438.1"
                     /translation="MKLFKTWYIEKYKTWDQTWWNNIVSTNKYNDPIVRKWSEEYGYN
                     LYEAWNKHMKNLGKKSAIIGLNGLTSDRFSNEFEYYYPSPMREYVVANYDLLFTYMDP
                     INSSEIIESENQVKGLRKKFNYGGKIAHILTSSFRNKQTWNETVAFEEFKIVHPYVDV
                     IMVYPYADEWNYPKIPYPPKLINFSRRFNEKNH"
     gene            complement(5755..>6158)
                     /locus_tag="VKL21_01320"
     CDS             complement(5755..>6158)
                     /locus_tag="VKL21_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HMB44439.1"
                     /translation="SMMAIGTLGIFSYMLSNEPQKAQTIAFTTFVFFQLFNVLNCRSN
                     IHSIFKIGFFSNRTSILSITGVILIQAAIVYLAQVQGVFGTVPLSFKDWLFSAAIASS
                     IFIAFEMSKSFKNKSLLFSMIFFIKSPGKIN"
BASE COUNT         1983 a         1099 c         1326 g         1750 t
ORIGIN      
        1 tatgtgattg gaattagatt taatcccgca catgtaaggg agtaaaaatt ttttaagaca
       61 gcaaacacag ctatcccgaa ttcgagataa atcaggcaat tgatatacat gtaaagcctt
      121 atgaggatgt ttgcagacat gagtaataac tatattttta gcaaactaaa atctggcaaa
      181 accttgatca attatgcttt cctgaaaata tccagccagg gattcttatt ccttctatct
      241 ctcattcttg caaaagtatt atctcctgat agattcgggg catattctct aagcctgatg
      301 ataatctatt tttttagtgc cctattcatg agatcctcac aggatccttc cattgtcctg
      361 ggcagcgacg agttgaaaca acatagcaaa attaacaaaa tatttactgt aagaattatc
      421 ttgctgatcc aggcaatcat tcttttttat ttgattacca gggtattctt ccagccatta
      481 tcgcattttt cttcccttga tgagactgga tttattttcc tgttttttgc gtattcaggc
      541 ttatgcatac gaaatttttt cgaaggtact tttttggccc tgaaccagag aatggccgag
      601 gctgcatacg atgccctcac caatataatt tcgtttgtct ggctatttgt aatctattat
      661 ttttttacta tcaatattga aaacatttta atgatgttct tcgtttcacc acttattgcc
      721 agtctttttc ttacaagaga gatagatttg aagaaagttc ttccaattga atttgacttg
      781 cgtatattaa ggcaaatgtt tgactatacc aaatggacta ttatgggtag tgttggtttg
      841 tatttggtaa attgggtgga tatttttgtc ttaagaatct ttgtctccct ggatcaaatc
      901 ggggtctaca atttaggata tcagatattc aagggagttc ttatcctcat ttctatcgcg
      961 agagtatatt atttgccttt catttccgaa catatcaagg atgaaaagaa aatgaaaaac
     1021 tacctgaaga acaagcgatg gataatgatg gctgccggat cgatcgggat atgtcttatc
     1081 tatattgctc ttccgtatct cttttctatc ttgtatgcag atacttatca gggttcggtc
     1141 actgttgcga ggatattact ggttggagcg gtattttccc tctaccaaac attttactca
     1201 cctattttcg atgccatgag acgctatgaa tttacccagc ttgcatcaat attaatggtg
     1261 gccatcaatc ttgcactcga ctatatcctg gtgttccttt atggattttt gggagctgct
     1321 gtggcaaccg ctatcacata tgcaatattg agcttgattt atgaaatgta tttcagaaaa
     1381 aacttaaaga atattatttt ttcatctccc cagcgcccta caatcagata acttcattaa
     1441 cctctattca aaatatatca atgatgtctg gtaagaatgt tacaaaagta ctcctgttcc
     1501 ttaaaaatat ggtatatgag agcacaagtc ccaatgaaat cctgcgtttt gcgaaatatt
     1561 atcgaaagaa aggactggat gtagttctgg ttctctgggg accgatgggt gttattcttg
     1621 gaaaaaaagg caagaccgga acgccagctt atgatgacag ggtaagggag tgtatcgaaa
     1681 tgggaataaa gatcaaatgc tgtgaacttg cttcgtcgat gataggttta gagaaagatg
     1741 aacttatcga aggtatagaa ctgatacact catttgaggt ggctgacatc atgcttgaat
     1801 actgtgaaga ggggcagctt gtaataaacc tttgaacgaa ggtaatatgt acaattattg
     1861 acctcataaa accaatgaaa ctgaatcttt ccgatgccca ggcattcctg atggtcagtg
     1921 agagcgaaca caacgcggat atgtattatg ccagcgggtt cctggcttat gatccgtttg
     1981 tttaccttaa ttccgggaca gatctcatcc ttatatctga tatggaactg ggaagagcaa
     2041 gaaaggaatc aaaagtccat gatgttatcc cgacatccgg attcaacatt atggataaga
     2101 tccgaaaaac cagggatgct gatgcagcat attgtgaaat gataactgag cttatccatt
     2161 caaagaattt gaaccgtatc gcagtgccat ataattttcc ggttcagctt gccgattgtt
     2221 tacggggggc aggacttgag atcatcccca taaaaagccc attacgcgaa atgcgcgaaa
     2281 taaaaaatga acgtgagata agtgcgatcg aatatgcaca aagagccggt gagaaagcgc
     2341 tcgctgaagg tatcagggca ataaaaaaca cagatgtgaa agatggcgtt ctcatgtatg
     2401 aaaattcacc attgaaaacc caggatataa gggctattat agacagatcg cttctttctt
     2461 tcaattgtga agcccctgac attattatcg cgtgtggtcg aggcagcagc gaccctcact
     2521 ggaaagggga aggtgagctt cttgcagatg agcccatagt cattgacatg gttccccgtt
     2581 ccaaaaagga caggtattat tcggatatga caaggacagt gatgcgcgga actccttcga
     2641 aagaattgaa agatatgtat tcggctgttc ttgattccca gatatgtgcc attaaaaaga
     2701 taaaagcagg agttacaggc gctgatatcc acaacatcgt atgcgatgta ctggaagaaa
     2761 gaggatacaa gaccgcaagg tgtaagaata ctgagttcac ggaaggtttc atccattcga
     2821 cagggcatgg cgtgggtctt gatatccatg agggaccaaa cctgggcgag aatggaaaag
     2881 aattaaaagc aggatgcgtt gttacggtcg aacccggtct ttattataag aaaatcggtg
     2941 gtgtgcgcct tgaggatgtt gtggttgtta caaggagcgg atgcaataat ttaactatgt
     3001 ttgaaaagaa tctgttatta taatgacaga aactgatgaa acaatcgaga aataccgaca
     3061 ggcaggaagg atcctgtccg aggtccgaaa acttacagcg gaaaaggtaa ctgagggcgc
     3121 aagtcttctt atggtcgcgg aattcgggga gaacctgata cgagaaaaag gcgcccaacc
     3181 tgcgtttccc gtgaacattt cacgaaatga cgaggcagct cattcaactc catgtttgaa
     3241 tgataaatcc gtatttggaa aagatatggt aaaattggat atcggggtac atatcgatgg
     3301 atacattgca gatactgcga tcactgttga cctttccggc aatcccgaac ttgttaaagc
     3361 tgctgaatct gcccttgaag aggcgatcaa tttcattaaa gcgggtgtaa atacttcgga
     3421 tattggcgga gttatcgaag atgcaataac ttcctatggc tataaaccca taatcaacct
     3481 cacaggccat gggcttgcac aatatatcca gcatgcaccg cctgcgatcc ccaataagag
     3541 aatgccggga ggtgtgatac ttgaagaagg ggatatcatt gcgattgaac ctttcgccac
     3601 aaacggcgcc gggaagatac atgatgccgg aagcgccgag atcttccata ttgtttccga
     3661 tagaccggtc cggcaccctt ccgcgaggaa attattgaag gagatagaaa aatataagac
     3721 gcttcccttt gcgaaaagat ggctgggtga gcgtgtggat tttgcaatgc ttgcactttc
     3781 caaagcaaat attatcaggc cgtacccgat actcaaggaa atccagggcg gtcttatctc
     3841 ccaggctgaa catacgatta tcgtgacagc ggatggatgc gaggtagtca cgatatgaag
     3901 atatcgggga tattcatatc attaattctt ctttttacag caacggattc agtcgcagca
     3961 gaaaacaggt gggtaaataa tataaccgta aatgaatata atattgcctg gggctacaca
     4021 gagactttcg ctggtatgga ttcaatagct tacaggatca accttgattc aaagatcgga
     4081 aataatgata gttttatcaa tgcatgggaa ctcctgaaag cggataagga ttcgcgagaa
     4141 aagttcaaga attccatgga aaatgaacct gacgtaagga taaataatga gacctctgga
     4201 atagaaatga tagatgttga ttcttcctta tcgcaggcca ttatcggaaa aacccatatc
     4261 gccgatacga tcgagaacag gtataaggtc acctataggt tgaaggaaag cattttcaac
     4321 gacagtagta tctggttcct gggagaacca gaatcaccca taacaataat tcttccccag
     4381 ggcatcgatg ttatcggtat ttcaggtatg aataatgaaa ccaagaatat cgactcgaac
     4441 acagaggtct caggatattt ctccaattca tctaatgaaa gcgcgcagat aaccgtcaat
     4501 ataagtaaaa atgcatcgta taacctgacg tccccggaaa ccaacataac agaagtcgtg
     4561 cctgcacaaa acaatgaaaa tattacagaa ccggcatccc aggtatctga gaaaatcagg
     4621 aaatggagta ttatcggggt gggaattgtt tttattgtat tgatttatgt ttttagagtg
     4681 agaaagaatt agcaagagat aggataataa gtcttatacg atttcgaagc tataactcat
     4741 gtgtagagca tgatcagaaa gttagtatgc attggattat tgacaattgt agtagttttt
     4801 ataaatggct gcgttcaaga aaaagttttt attaaacctg gggaaaccat aaaaccaaat
     4861 agtgttgagc tcggtaattt cggatatagt tattatgacc aggcggttcg tgattttcaa
     4921 aaccaacccg aaattagctg ggatgtaata ttaaatcaga atggattagt ttataaaaga
     4981 caaaatacaa ctgttgtctt ccaggattac atagaccaaa aaaagtttga aatgacagac
     5041 tttagtcgaa tccgaagccg ctttgatata gccaccaaat ataaagataa atatgattat
     5101 ggggtgtttt tattactgga agaggttttt cacccagtta taagagtgga atggggatgg
     5161 tcaaatatga gtaactggaa aatagaaggc tcggacaatt ttgaaggcgt aaaggtgaaa
     5221 ctgtttaaaa cctggtatat tgaaaaatac aaaacatggg atcaaacctg gtggaataat
     5281 attgtttcaa ctaacaaata caacgacccc attgtcagga aatggagtga agagtatgga
     5341 tataatttat acgaagcatg gaataaacat atgaagaatt tgggtaaaaa gtctgcaata
     5401 ataggcttaa atggattaac atccgaccgt ttttcaaatg aatttgaata ttattaccca
     5461 tcacccatgc gggagtatgt tgtagccaat tatgatttat tattcactta tatggatccg
     5521 ataaattcaa gcgaaatcat agaatctgaa aaccaggtta aaggtctgag aaaaaaattc
     5581 aattacgggg ggaaaattgc ccatatccta acctcttcat tcaggaataa acagacatgg
     5641 aacgaaactg tagcatttga agaattcaag attgttcatc catatgttga tgtgataatg
     5701 gtatatccat atgcagatga atggaactat cccaaaattc cctatcctcc taaactaatt
     5761 aatttttcca ggagatttaa tgaaaaaaat cattgaaaaa agcaacgatt tgttcttgaa
     5821 tgatttggac atctcaaatg caatgaatat gctggaagct atagctgccg agaatagcca
     5881 atctttgaat gacaaaggta cagtcccgaa tacaccctgt acctgtgcaa gataaactat
     5941 tgccgcctgg ataagaatta ctcctgtaat tgacaggata gatgttctgt tagagaaaaa
     6001 gcctatcttg aaaattgaat ggatattaga cctgcaattc aagacattaa aaagctggaa
     6061 gaaaacaaag gtcgtgaatg ctattgtttg tgccttttgg ggttcattgg agagcatgta
     6121 agagaatatc cccagtgtac caatagccat catggacc
//
LOCUS       DATGBN010000061         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_159712, whole genome shotgun sequence.
ACCESSION   DATGBN010000061 DATGBN010000000
VERSION     DATGBN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159712"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..421)
                     /locus_tag="VKL21_01325"
     CDS             complement(110..421)
                     /locus_tag="VKL21_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44440.1"
                     /translation="MKKILIIGLLVIFVLISGCTTMTTTRPAEPTPGATTPAQISTEQ
                     QINIRDLKFVPDIAEVPMGTTVTWVNDDNVPHTITSVSGSFDSGSIAPGRQFLSNAPK
                     R"
     gene            complement(489..668)
                     /locus_tag="VKL21_01330"
     CDS             complement(489..668)
                     /locus_tag="VKL21_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HMB44441.1"
                     /translation="MKSSTRDEVESKVHKIKGEIKKTAGQLLDNPKLEAEGTEEKIVG
                     KVQEKTGQIKKVFGK"
     gene            complement(727..1422)
                     /locus_tag="VKL21_01335"
     CDS             complement(727..1422)
                     /locus_tag="VKL21_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44442.1"
                     /translation="MYLFSWDNVPGKENGKLIEYLEQKYGVDWVRTAKIEKIDNNEIK
                     VANEKNFLSLRLNNENTKVTLTINKVKTDEFIVKTENSKLNIYTDIEQEKKDIRQGVK
                     VKEISYPYSVLGGLVLAGLIIVALMGYYGTQSAGIFSPREAFFVIGASILILSAASVY
                     QFMTLRDTISVLKIHEEELQSARDTAQEMRLASLESTRSGATTSLAPRVAALEPQLAT
                     LEKRVTALEVKRP"
BASE COUNT          412 a          355 c          335 g          594 t
ORIGIN      
        1 taagagtgtt tgcgcctatc tcaataaccc gtctcaatta ttaaagtttc atacttgaca
       61 agtgagtgag tttatgaaac aaatagtagg cgttccaaat ctgcttattt taccttttgg
      121 gtgcgttgct caaaaactgc ctacctggag cgatacttcc tgaatcgaat gaacctgata
      181 cggaagttat tgtgtgtgga acattatcat cattaaccca ggtcaccgtt gttcccattg
      241 ggacttctgc aatatcggga acgaatttta agtctctaat atttatctgt tgttctgtgg
      301 atatctgtgc tggtgttgtt gctcctggtg ttggttccgc aggtctggtc gtagtcatag
      361 tggtacatcc gcttattaat acaaatatca ctaacaatcc aataatcaat atctttttca
      421 tgttaaacct cccttacatc atgagattcg cacgtcctca aggacatgca ggtgaataat
      481 acgtagttct atttcccaaa gaccttcttg atctggccgg tcttttcttg aactttgccg
      541 acaatttttt cttctgtgcc ttcggcttcc agtttgggat tatcgagtag ctgcccggca
      601 gttttcttga tttcaccctt tattttgtga actttgcttt caacctcatc tcttgtgctg
      661 cttttcatag tattcctctg tttattattt atctgactgt ttttgcctat ttgcgctccg
      721 gctcacttag gggcgcttga cttccaaggc ggtgacgcgt ttctccaggg tcgcaagttg
      781 tggctccagg gcggcgacgc gaggggcaag cgaggttgtc gcgccggagc gcgtgctctc
      841 aagtgaggcg aggcgcattt cctgcgctgt atcgcgcgcg gattgcagtt cctcctcgtg
      901 gatcttgagg acgctgatag tgtcccgcag tgtcatgaac tggtacaccg atgcggcaga
      961 caggatgagt atggaagctc cgatcacgaa gaaagcttcg cgcggggaga atatgcctgc
     1021 gctttgggtg ccatagtagc ccatcaaggc cacaataatc aagcccgcga ggaccagtcc
     1081 gcctagtact gagtaggggt agctgatttc tttcactttg actccctgcc tgatatcttt
     1141 cttttcttgc tcaatatctg tgtatatgtt tagcttacta ttttctgtct tcacaatgaa
     1201 ttcatcagtt ttaaccttgt taattgttaa ggttacctta gtgttttcat tgttaagtct
     1261 tagcgaaaga aaattttttt cattagctac ttttatttca ttgttatcga ttttttcaat
     1321 ttttgcggtt ctaacccaat cgacaccata tttctgttcc agatactcta tgagttttcc
     1381 attctctttt cctggaacgt tgtcccagct aaataaatac atattttcac tccttgcttg
     1441 atagcaatta actaaagaaa acaatcattt ccaaataaca caaagatata ttagaaaaca
     1501 attgtttacc ccaataaggt tatatgcatt atcctatata aattttatgg atacgaccag
     1561 tatctccgaa agaatgttaa aatcttcttg aagttggaat tttttgtttt aattggtgcg
     1621 ttgttaccta attgttcaga acaatttgaa tttttataat gtaatacttt atatacattt
     1681 atatcataat ataaat
//
LOCUS       DATGBN010000062         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_4337, whole genome shotgun sequence.
ACCESSION   DATGBN010000062 DATGBN010000000
VERSION     DATGBN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4337"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VKL21_01340"
     CDS             complement(<1..267)
                     /locus_tag="VKL21_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44443.1"
                     /translation="MNKITIMVVTAIIVVLGAGYAVARPESSGMMGGNGMMGSDGNRV
                     TGGLSSMMGQYAGMMSDFGSGIWNSMMGYGNGATAGYGGMMGGNG"
     gene            complement(403..543)
                     /locus_tag="VKL21_01345"
     CDS             complement(403..543)
                     /locus_tag="VKL21_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44444.1"
                     /translation="MLETSEERVELLKAGIFDKIIEELYISGNNIKILKHHVLFELNN
                     KK"
     gene            complement(762..>1689)
                     /gene="mqnC"
                     /locus_tag="VKL21_01350"
     CDS             complement(762..>1689)
                     /gene="mqnC"
                     /locus_tag="VKL21_01350"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HMB44445.1"
                     /translation="ALVTFVIDRNINYTNICTSKCKFCAFYREPDAKDAYVLSIEDIL
                     EKVDEAVKLGATQILIQGGLNPDIPIEYYEKMLERVKNKFNVQMHAFSPPEIVHISKI
                     YGSTIKETISRLHDAGLDSIPGGGAEILDDRVRNSVSPKKIGWKKWQEVMLAAHSLGI
                     PTTATMVFGHEETTLERIKHIIRVRDMQQEYHGFTAFIPWSFQSENTELKGASTGIDY
                     LKMVAVSRILLAGHIKNIQASWVTQGIDVAQMALDFGANDLGGTMIEENVVRAAGVPF
                     HSKTIDDFIHAVRKLGRPVGKRDTLYNLIERY"
BASE COUNT          456 a          390 c          258 g          585 t
ORIGIN      
        1 accattaccc cccatcatac cgccgtaacc tgcggtcgct ccattgccgt aacccatcat
       61 gctgttccat atgccacttc cgaagtcgct catcatacct gcatattggc ccatcatact
      121 gctcaagccg cctgttactc tattgccatc gcttcccatc atcccgttgc cgcccatcat
      181 accgctgctc tctggtcttg ccacagcgta acctgctccc agaactacaa tgatcgctgt
      241 tacaaccatg atcgttattt tgttcatatt tatttcacct ttggtcaatt atttatttca
      301 tgtgtaagtt ttacttacat actataatta atattactat ggtataaaga tttcgttagg
      361 caataatacc ggaaatatct ccataatatc tatgaaaaga cactatttct tattatttaa
      421 ttcaaaaaga acgtgatgct tcagaatttt gatattatta cctgaaatat aaagctcttc
      481 aattatctta tcaaagatgc ctgcttttaa taattctacc ctttcttcgg aagtttctaa
      541 catcttgagt tttcctaatt aaatcatatt taagacaaaa cgttattgtt taaacattaa
      601 ttatccatta attaaccttt actctgctag ccttttttcc cttttttttg ttagatatta
      661 ttaattacat tcttgtttat aaatatttct atgaaatttt tcaaaatcat acaatttagg
      721 ttattggctt gagtgaacta cccaaaataa agtataatgc ttcaatatcg ttcaattaga
      781 ttatataatg tatccctttt tcccactggc ctgcccaatt ttcttactgc atggatgaaa
      841 tcgtcaatgg tcttgctgtg aaagggcacg cctgccgccc ggacaacatt ttcctcgatc
      901 attgtcccgc caaggtcatt tgcgccaaaa tccaatgcca tttgcgcaac atcaattccc
      961 tgggtaaccc aggaagcctg aatattcttt atatggccgg caagaagaat tcttgaaact
     1021 gcaaccatct tcagataatc tatccccgtt gaagctcctt tcaattcagt attctcagat
     1081 tgaaaactcc aggggataaa tgctgtaaaa ccatgatatt cttgttgcat gtctcgaact
     1141 cttatgatat gttttatcct ctctagcgtt gtctcctcat gcccgaacac cattgtagcc
     1201 gtagtaggaa ttcctaatga gtgcgctgcc agcattacct cttgccattt cttccatccg
     1261 atctttttag gtgaaacaga attccttact ctatcatcaa ggatctctgc tcccccacct
     1321 ggtatggaat caagcccggc atcatgcaga cgcgaaatcg tttcctttat cgtggagcca
     1381 tatatctttg agatatgaac gatttcgggt ggtgagaatg catgcatctg tacattgaat
     1441 ttatttttga ctctttctag cattttttca taatactcta tgggtatatc gggattcaat
     1501 cctccttgta tgagtatctg ggtcgccccc aatttaacag cttcatccac cttttccagt
     1561 atatcttcaa tacttaatac ataagcatct tttgcatcgg gttcccggta aaaagcacag
     1621 aatttgcatt tggatgtgca gatgttagta taatttatgt ttcggtctat aacaaatgtg
     1681 acaagggca
//
LOCUS       DATGBN010000063         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_159733, whole genome shotgun sequence.
ACCESSION   DATGBN010000063 DATGBN010000000
VERSION     DATGBN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159733"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1393
                     /gene="lysA"
                     /locus_tag="VKL21_01355"
     CDS             113..1393
                     /gene="lysA"
                     /locus_tag="VKL21_01355"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMB44446.1"
                     /translation="MFTIKDHLEIRNNHLIIGGADTTVLAEQFGTPLYVTNESRVISN
                     FSAYRNAFPDAHIYYAAKANGSFAILRMLARQGAGADVFSFGELYIALLAGISPQKIL
                     FNGNSKTDFELQKAVEIGIKVSVDSLDELHTLSKIADSLGKTVEIAFRVNPDVSPNTH
                     PKISTGLRTSKFGIPSREVVGAYQEADKLPGITLSGIHCHIGSQILDTAPFTEAAQKM
                     MDLVEEITGLGINLKFVDMGSGLGIPYEKGGKVPTPREHAKAILPVFEERTRALGISP
                     KLILEPGRFIVADTTILLTRVNTVKKADKNFVGVDAGFNLLVRPTMYDSYHHVVVANK
                     VKSQAEDTYTIVGPICETGDIFAKDRTLPHIEKNDVLALLDAGAYGFSMSSQYNGRPR
                     CAEVLVKNGEADVIRSREDFEDLLTGQKLPARLM"
     gene            1402..>1688
                     /locus_tag="VKL21_01360"
     CDS             1402..>1688
                     /locus_tag="VKL21_01360"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HMB44447.1"
                     /translation="MIDPVDNFEIPVEWINRASLPPDELRSRILSGRYVLLSDGSLLR
                     RGYTTGTTAAAAAKAAVISLVRDISETSVLTPVGIRVFLPVHARKGKASAI"
BASE COUNT          477 a          368 c          389 g          454 t
ORIGIN      
        1 aacccgctta tcttgttcgc tattaaacga acgcgggtat tatgaaaggc tctaaataaa
       61 tagttttcgc tcaaagataa gctatattat aatatccgtt aattgtgctg ctatgttcac
      121 aataaaagat catcttgaaa tccggaataa tcatctcata attggcggcg ctgacacaac
      181 cgtccttgca gaacagtttg gaactcccct gtacgttacc aatgaatccc gtgtaatcag
      241 taatttttcg gcatatagaa atgctttccc agatgcgcat atctattatg cggccaaagc
      301 aaatggaagc tttgccatcc tcaggatgct tgcacggcaa ggggctggcg ccgatgtttt
      361 cagtttcggt gagctttata ttgcgcttct tgccgggatt tcgcctcaga aaatcctctt
      421 caatggcaat tcaaagactg attttgagct gcaaaaggct gtggaaatag ggataaaagt
      481 ctctgttgat tctctggatg aacttcatac attatcaaaa attgctgatt ccctgggtaa
      541 aaccgtagag atcgcattcc gcgtcaatcc tgatgtctct ccaaatacgc acccgaaaat
      601 atcaaccggc cttcgaactt caaaattcgg aattccttcc cgggaagttg taggtgccta
      661 tcaagaggcg gataagcttc cgggtatcac actttccggt attcattgcc atattggatc
      721 ccagatattg gatacggcgc ctttcactga agccgctcaa aaaatgatgg atcttgtgga
      781 agaaatcaca ggacttggga taaatctcaa attcgtggac atgggttcag gtcttggcat
      841 cccatatgaa aaagggggga aagtaccaac tccacgtgaa catgcaaaag cgatccttcc
      901 cgtatttgaa gaaagaacaa gagctttggg aatttcgccc aaacttatac ttgaacccgg
      961 gcgtttcatt gttgcagata ccactatcct cctgacgcgc gtcaatacgg tcaagaaagc
     1021 agataaaaat tttgtgggtg tagatgcggg ttttaatctt cttgtccgcc ccactatgta
     1081 tgactcatat catcacgttg tagttgcgaa caaagtgaaa tcacaagcag aagatacata
     1141 tacgatcgta gggccaatat gcgaaacagg agatattttt gcaaaggata ggactctgcc
     1201 gcatattgaa aagaatgacg ttttggccct gttagatgcc ggtgcatatg gtttcagcat
     1261 gagcagccag tataatggga gacccaggtg cgctgaagtg cttgtaaaga atggagaagc
     1321 agacgtcatt cgaagccgtg aggacttcga ggatttactc accggacaga agcttccggc
     1381 acggctgatg tgaatataaa gatgattgac ccggttgata attttgaaat tcccgttgaa
     1441 tggataaatc gagcaagttt accacctgac gaacttaggt ccaggatact gagcgggcgt
     1501 tacgttcttc tttcggacgg ctcgcttttg cgacgcggat atacaacagg gaccactgcc
     1561 gctgcggctg caaaagctgc tgtcatatca ctggtcaggg atatttcaga aacatcagtt
     1621 cttacacctg ttgggatccg cgttttcctt cctgtccatg caagaaaagg gaaagcttcg
     1681 gccattaa
//
LOCUS       DATGBN010000064         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_237530, whole genome shotgun sequence.
ACCESSION   DATGBN010000064 DATGBN010000000
VERSION     DATGBN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237530"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..741
                     /locus_tag="VKL21_01365"
     CDS             328..741
                     /locus_tag="VKL21_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HMB44448.1"
                     /translation="MSAESGNSTGNNSTINNPELKKINLQGLAFFTEVEPGLLDAKSQ
                     GKPVFVYARSKYCGACKQFEAETFTNSSVIDKLNKDFILISIDVDEQKTETRNFRLRA
                     TPTEIFLDPNGTEIKRLLGYRTNQTFLDEINKIVI"
     gene            782..2050
                     /locus_tag="VKL21_01370"
     CDS             782..2050
                     /locus_tag="VKL21_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HMB44449.1"
                     /translation="MKPEKKQDTEKKSKKMPKKKGNNSIILIAVAAVILIGAYALFSG
                     GSQTTEPKQTMTLKEVRALIDKEYGNKAPGTNTPPVKDNYLPILIPRKADKLPDFAVT
                     NAMTLKAYKYATEHPEVLEQIPCYCGCGEHGSVTSGGKPHMSVRDCFISNDGVYDDHA
                     SFCDVCVGIASKAQSYFPAGIPVTGLSAATPAPSIDLSTLSLSENFKSIADGLKLTQS
                     GINRAYFVNTKLITGTELENQLLNKQVQPDSFYGKKIIGMFSADYSTNSWIELHDLGY
                     DSKNDGALKGRTEPGMKNIVITRPLVYGHSQNVDNVLKLISDPNSMNTSYPAFKPLLD
                     AVDYQNAAYTIVIETKNKFSDINYMGLTPAGGKIELVRAFNVTDSKSIPSGFSKYNPE
                     TKGNILVIKTTGDYAAVMAENDNIDAVAIT"
     gene            2266..3642
                     /locus_tag="VKL21_01375"
     CDS             2266..3642
                     /locus_tag="VKL21_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB44450.1"
                     /translation="MEKNRNDKKYDLLDVYFFKDLVKNRFFKPAVNFFFFIFLLIIIY
                     FGLSSEPDLRFHGGIPFATTMIWDLWHPLLAFTLILVGRLWCFACPIGAIGEWTQSFF
                     SLNRKYPEKYRNLWIAVILFLVIFAGERHLFQFTRNPPNTAYLLLFFTGLAIVMGAVY
                     EKRSFCRYICPIGLVLGIFSMLSAIELRCKSRKTCHDHNIKECIIGSESGKACPVGEF
                     PQTMERNNQCLMCMECVKSCSKGNIRISPRIPGADIIHSRKTHLDEAYLVHGIIIIFL
                     FVMGMERLQFRNVIINFVKSTLPLDSLTFLDLYWRNMWAVIIFILITLGAAGLMYLST
                     RAAFSGKNTKQKFIELSYAFIPLSLSVYLAENTFRLLKGLFFIASSIGNFFGRIWEFS
                     PDFDTINFMQILLLAAGFIFTLWAGHLISKRDSQDENELHQSMITVGFTAFVYLIIGF
                     KILTLPII"
     gene            3776..4264
                     /locus_tag="VKL21_01380"
     CDS             3776..4264
                     /locus_tag="VKL21_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMB44451.1"
                     /translation="MRTKKLLSIFVLVLILSFAYFSYKTALNNFTLSDKNSSYLGGIK
                     FHRAAEGLEESFQIAQQENKPVMMYFWAIWCQYCARFQTDTLGNPQVKKILEEEYVLV
                     AMDLDIDREVAQKYGVSYPPYLVFMDPKGNVLQRVPGAVDANYLLPIVTQVRDQVRKK
                     IT"
BASE COUNT         1399 a          831 c          862 g         1187 t
ORIGIN      
        1 atccctgtga aaattaatca cctgaacccg gataagtttg catttaagaa aaccaacctg
       61 caaataaaat tctaacaagc cccataattg ccacgagaga tgttgggcag aagcgggatc
      121 gaaaaatcct gcttggaagt atggcagata aagtgaaccg tgatttaaat ttcccagtga
      181 tgacggtaag aaaatagcta ttgttcatac ttaatttgca aaaataccac aatccttata
      241 taactttaca ttcatcgtaa aataggatag aacttgaaaa aatgtatata tttaacaatt
      301 gttctctttg tcttactcac aggatgtttg agtgccgaat cgggtaattc aacaggtaat
      361 aattcaacca taaataatcc tgaactgaaa aagataaact tacagggatt ggcctttttc
      421 acagaagtcg agcctggcct tcttgatgca aaatcacaag gaaagccggt gttcgtatat
      481 gcaaggtcga aatattgcgg cgcatgcaaa caattcgaag cggagacttt cacaaattca
      541 agcgtgatag ataaactgaa taaggatttc atattgatct cgatagatgt ggatgagcaa
      601 aaaacagaaa ccagaaattt cagattaaga gccactccta ccgaaatatt cctggatcca
      661 aatggaaccg aaataaaaag attacttgga tacaggacca accagacatt cctcgatgaa
      721 ataaataaga tcgtaatttg aatttagaaa aaaatattat aaaaataaat gaggcaatac
      781 aatgaagcct gaaaaaaaac aagatactga aaagaaaagc aaaaagatgc ctaaaaagaa
      841 aggaaataat agcataatat taattgcagt agctgcagtt attctcatag gagcttatgc
      901 cttattttca ggtggatcgc agaccaccga acccaaacag acaatgacgc ttaaggaagt
      961 aagggcattg atcgacaaag aatacggtaa caaagccccg gggacaaata cgcctcctgt
     1021 caaggataat tatctcccga tacttatccc aagaaaggcc gataagcttc cggattttgc
     1081 cgttactaac gccatgacat taaaagcata taaatatgcg acagaacacc cggaagtact
     1141 tgaacagata ccctgttact gcggatgtgg cgagcatgga agtgtaacat ccgggggaaa
     1201 acctcatatg tctgttagag attgctttat ttccaatgat ggggtctatg acgaccatgc
     1261 ttctttctgt gatgtatgtg tcgggatagc ctcaaaagcc cagagctact tccctgccgg
     1321 aattcctgtt acaggattat ctgctgccac acctgctcca tcaattgatc tatcaactct
     1381 ctccctgtca gagaatttca agtccattgc agatggcttg aaactaacgc agtctggcat
     1441 caaccgcgca tactttgtta atacaaaatt gatcactgga acagaactgg agaaccaatt
     1501 attaaataag caggtacagc ccgatagttt ctatgggaag aaaataatcg gcatgttcag
     1561 tgcggattat agcacgaatt cctggatcga acttcacgat ctgggttatg acagcaaaaa
     1621 tgatggggca ttaaaaggaa gaacagaacc tggaatgaag aatattgtaa ttacaagacc
     1681 actggtttac ggacatagcc agaatgtgga caacgtttta aaattaataa gtgacccgaa
     1741 tagcatgaat acatcgtatc ctgcattcaa accactgctt gacgcagttg attaccagaa
     1801 cgctgcgtat acgattgtca ttgaaacgaa aaacaagttc tcggacatca attacatggg
     1861 tttgaccccg gcaggaggaa aaatagaact tgtcagggca ttcaatgtaa cggattccaa
     1921 atccattccc tcaggtttta gcaagtataa tcccgaaaca aaagggaata ttcttgttat
     1981 caaaacaacg ggtgactatg cagccgtaat ggccgaaaac gataatatcg atgcagtagc
     2041 tataacataa aatatcagta gcctcccata ttactgatat ttctcgcagg catggcaact
     2101 gtggcgaatc tgtgcgttct tccaatcctg cctgcggtat tatcaggttc agtaggggca
     2161 ggcttcacca tataaagaca ctattccttc cttattccgc aaatattgag actgaaattt
     2221 caaaggcata tgaatatctc ggtagttatg ggaactaata accacatgga aaaaaacaga
     2281 aatgataaga aatatgacct gcttgatgta tattttttca aggatcttgt gaagaatagg
     2341 ttctttaagc ctgcagtcaa cttttttttc ttcattttct tgcttattat aatttacttt
     2401 ggtctttcaa gtgaacccga cttgagattc catggaggga taccattcgc cacgaccatg
     2461 atatgggacc tatggcatcc gttacttgca tttactctta tacttgtggg gcgcctgtgg
     2521 tgttttgcct gccccatcgg agcgatcggc gaatggacgc agtccttttt cagcctgaac
     2581 aggaaatatc ctgaaaagta tcgcaatctc tggatagccg taatactttt cctggtaatt
     2641 ttcgcaggtg agcggcacct gttccagttc acaagaaatc ctcccaatac tgcatatctt
     2701 ctcctgtttt ttacaggtct tgccatagtg atgggagcag tctatgaaaa acgaagcttc
     2761 tgccggtaca tctgccccat agggcttgta ctcgggatct tctcaatgct ctcggcaatt
     2821 gagcttcgat gcaagtcaag aaaaacatgc catgatcata acatcaagga atgcataatt
     2881 ggcagcgaat ctggaaaagc ctgccctgtg ggtgaatttc cccagacaat ggaacgcaat
     2941 aaccagtgcc ttatgtgcat ggaatgcgta aaatcatgtt caaagggaaa tatccggata
     3001 agccccagaa ttccgggagc agatataatt cattcaagaa aaactcatct tgatgaagca
     3061 tatcttgtcc acgggataat cattattttt ctatttgtga tgggaatgga acgattgcaa
     3121 ttcaggaatg tgatcatcaa ttttgtcaaa tccactcttc ctttggattc actcactttc
     3181 cttgacctgt actggagaaa tatgtgggca gttatcatat ttatcctgat aacacttggc
     3241 gcggcaggat tgatgtatct ttccacaagg gcggcatttt ccggaaaaaa tacaaaacag
     3301 aagtttattg aattatctta tgctttcata cctttgagct tatcggtcta tctggctgaa
     3361 aatacttttc gccttttaaa aggattgttt ttcatagcgt ccagtattgg aaattttttc
     3421 gggagaatct gggaattctc acctgatttt gatacaatta acttcatgca gatactcttg
     3481 ttggctgcgg gatttatctt taccttatgg gcagggcatc ttatcagcaa gagggattca
     3541 caggatgaaa acgaattgca tcagagtatg atcacagtcg gatttacagc ttttgtttat
     3601 ttgatcattg gatttaagat tttgacatta cccataatat aaatttactt tacacacacc
     3661 gtaaagtata tagtagaaca ggagaaatac aactaatatt cactgcttaa taaagaaaaa
     3721 ctcaagataa aaacatatat gccgatattc gattcaaaat gttaggaaaa aacatatgag
     3781 aacaaaaaaa ctgttgtcca tatttgtcct ggtattaatt ttatcattcg catatttcag
     3841 ttataaaact gcacttaata attttacctt gagcgataag aacagcagtt atcttggcgg
     3901 cataaaattc catcgcgcgg cggaaggact cgaagaaagt tttcagatag ctcagcagga
     3961 aaataaacct gtcatgatgt atttctgggc aatctggtgc cagtattgcg caagattcca
     4021 gacagatact ctggggaatc ctcaggtcaa gaaaatcctt gaggaagaat atgttcttgt
     4081 agcaatggac cttgatattg atagggaagt cgcccaaaaa tatggggtga gttatcctcc
     4141 atatctagtt ttcatggacc ctaaaggaaa tgtcctccag agagttccag gggcggttga
     4201 tgcgaattat ttactaccaa ttgttactca ggtaagagac caggtcagga aaaaaataac
     4261 atgataccat taggagaca
//
LOCUS       DATGBN010000065         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_548184, whole genome shotgun sequence.
ACCESSION   DATGBN010000065 DATGBN010000000
VERSION     DATGBN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548184"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="glyS"
                     /locus_tag="VKL21_01385"
     CDS             complement(<1..665)
                     /gene="glyS"
                     /locus_tag="VKL21_01385"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMB44452.1"
                     /translation="MADKYEQVLELAKRRGFLWNSFELYGGTAGFFDYGPLGAMLKRR
                     VENIWRDIFVINEGYYEIEAPTIGIEDIFIASGHVGGFSDPLTECQKCHEAFRADHLV
                     QDLVENPDTLSAEELTDVICKNNVKCPECGGNLGSAYEFNLMFKTSIGPGGKRMGYLR
                     PETAQGMFVDFPRLLKFYRDHLPFGATQIGKAYRNEISPRQGVIRLREFTQAEAEIFI
                     DPR"
     gene            810..2021
                     /gene="xseA"
                     /locus_tag="VKL21_01390"
     CDS             810..2021
                     /gene="xseA"
                     /locus_tag="VKL21_01390"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMB44453.1"
                     /translation="METKGIYTVHEFTLAVKSILTTGILNDTWIRGEISNFTNHNSGH
                     RYFTLKDKNSQLQCVMFKWYGKNLRFEPEHGMKVLVFGDLDVYEQKGVYQLKVRDIRP
                     DGIGELYKAYEQLKNKLAMEGLFSPDYKKPLMRFPKKIGVATSPTGAVLHDMLTVLGR
                     RYPVNILFIPTVVQGDSAAESIVKSILALNETDVDIIILGRGGGGIEDLWAFNEEVVA
                     RAIFNSRIPIISAVGHETDYTIADFVADIRAPTPSVAAEIAVPDRLELANHITRLWQR
                     LIENQRRFISDRKSHLSDLEEDVEPCLFVERLTRHIQFIDDLTLRQELSIGRLIDIKK
                     ALLDANAGKLNAVSPLGTLSRGYGITLKLPGKTLVQSIEDVERSDEVSIIVRDGKIRC
                     NVNDKEECKWK"
     gene            2012..2230
                     /locus_tag="VKL21_01395"
     CDS             2012..2230
                     /locus_tag="VKL21_01395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HMB44454.1"
                     /translation="MEMSFEESLTELEGIVDKLEKGQLSLDESLMLFERGIKLVRECN
                     TKLKSAQQKVEQLIVENGGCRTEKFEVD"
     gene            2320..3240
                     /locus_tag="VKL21_01400"
     CDS             2320..3240
                     /locus_tag="VKL21_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44455.1"
                     /translation="MKNDRILTGIYGLDDLMDGGFRKNTINSIYGGPGVGKTTFALQY
                     SLFGLDRGEKALYISFEMSEKQIIRDCTDIGWGVIKDYIEEGDLKILHIFGEDLEFPS
                     LDITEIIKKSISRHNPRIVIDPFTYPTFYSDKEKRKSISTIFQDLRTLGTTVVVLEEP
                     ADGVTATNFGGSGEIQSTNKASSMPLYLSDTVLFLQNLGFGEMYDRTIRIMKHRGSKH
                     GEGIYPYSIETGLGLVLRASKKQIDRVVPKTKFDERFVQALDKTKSMGELGKKLAVRI
                     EALRKNWVHDDDPQHILEMVLKQEKKGNDN"
BASE COUNT         1048 a          618 c          797 g          901 t
ORIGIN      
        1 tccctcgggt ctatgaatat ctctgcttca gcctgcgtga attcacgaag gcgtatcacg
       61 ccctggcgcg gggagatctc atttcggtat gctttgccaa tctgtgttgc gccaaatggc
      121 aggtggtcgc ggtagaattt aagaagacgg ggaaaatcca cgaacattcc ctgtgcagtt
      181 tcggggcgaa gataacccat tcttttcccg ccaggtccta tggatgtttt gaacataaga
      241 ttgaactcat aggcgcttcc gagatttccc ccgcattcag ggcattttac attattttta
      301 cagattacat ccgtcagttc ttcagcgctc agggtatctg gattttccac taaatcctga
      361 acaagatgat ctgccctgaa ggcttcatga cacttctggc attcggtaag aggatcagag
      421 aatccgccta catgacctga tgcaataaag atatcttcaa taccgatcgt tggcgcttct
      481 atttcataat aaccttcatt tatcacaaat atgtcgcgcc agatgttctc aacgcggcgc
      541 tttagcattg cgccaagcgg tccgtaatcg aaaaaacctg cggtcccgcc atacagctca
      601 aatgagttcc ataagaatcc gcggcgcttt gccagttcaa gcacctgttc gtatttgtca
      661 gccatgatgg cttaatacat gttttggatt attaagtttt atcggaatct tgaataaggt
      721 taaacagaga tgtttagatt caagatttgt aaagattagt ttcactccgc tatcgcaaag
      781 cagataagtt caaggatcaa tctggaagca tggaaacgaa aggcatctat actgtacatg
      841 aattcacatt agcagtaaag agcattctta ctacgggtat attaaatgat acctggatcc
      901 ggggggaaat atcgaatttt accaaccata attcaggaca tcgctatttt actctgaaag
      961 ataaaaacag ccagttgcag tgtgtcatgt tcaaatggta tggcaagaac ctcaggtttg
     1021 aaccggaaca tggcatgaag gtcctggtat tcggggatct ggatgtatat gaacagaaag
     1081 gtgtttacca gttaaaggtc agggatattc ggcctgacgg tataggagaa ctctacaaag
     1141 cctatgagca attgaagaat aaacttgcca tggagggact tttttcacct gactataaaa
     1201 aaccgctgat gaggttcccg aagaaaatag gtgttgcaac atccccaact ggcgccgttc
     1261 ttcatgatat gctcacggtg cttggaagga ggtatccggt caatattctt tttatcccga
     1321 cagttgtgca gggagacagc gcagcagaaa gcatagtgaa atcgatattg gccttaaatg
     1381 agaccgatgt ggatataatt attctgggca gaggcggagg cggaattgaa gacctctggg
     1441 cttttaatga agaagtggtc gcccgcgcga tttttaattc aaggatcccg ataatttcgg
     1501 cggtggggca tgagaccgat tatactattg cagattttgt agccgatata agggcgccta
     1561 cgccgtcggt tgctgcggaa attgcggtcc ctgaccgcct tgaactggca aatcatatca
     1621 ccaggctgtg gcaaaggctg atagagaacc agaggcgatt tatcagcgac aggaaatcac
     1681 atctttctga tctggaggag gatgtcgaac catgtttatt tgttgaacgc ttgacccggc
     1741 atattcaatt tattgatgat ctgacactaa gacaggaatt gagtattggc agactcattg
     1801 atattaaaaa ggctttattg gatgcaaatg caggaaaact taatgcagta agcccgcttg
     1861 gtacactttc aagagggtat ggcattacat tgaaactacc agggaaaaca ctcgttcaaa
     1921 gcatagaaga tgtggaaaga tcagatgagg tttcgataat tgtgagagat ggaaaaatca
     1981 gatgcaatgt taatgataaa gaggaatgca aatggaaatg agttttgagg aatcacttac
     2041 agaacttgag ggaattgtgg ataaacttga gaaagggcaa ctttcacttg atgagagcct
     2101 tatgcttttt gagaggggga tcaaacttgt aagggagtgc aatacgaaat taaagagcgc
     2161 acagcagaaa gttgagcaat tgatcgtgga aaatggggga tgcagaactg aaaagtttga
     2221 ggtggactaa tgtaccatat tactgatcgc attactaata ctaaactata aataatattc
     2281 agagcatatc aagcagcatt attataatga ggttaaagta tgaaaaatga taggatttta
     2341 acagggatat atggcctgga tgacctgatg gatggtggct tcaggaagaa tactatcaat
     2401 tcaatatatg gtggaccagg tgtcggtaaa accacatttg cattgcaata ttcgcttttt
     2461 ggattggatc gcggtgagaa agccctttat atttcctttg agatgtcaga gaagcaaatc
     2521 atccgtgatt gcacagacat aggatgggga gtcataaaag attatattga ggaaggtgat
     2581 ctgaagattc tccatatttt tggagaagac ctcgaattcc cgagtcttga tattacggag
     2641 atcatcaaga aatcaatttc cagacacaat ccaagaatag tcattgatcc ttttacatat
     2701 cctacttttt attctgataa agagaaaaga aaatctatca gtacgatatt ccaggatctt
     2761 cgaacacttg gaaccacagt tgtagtactg gaagagcctg ccgatggtgt aactgccaca
     2821 aatttcggag gttcaggtga aattcaaagt actaacaagg cttcatccat gccattatat
     2881 ttatctgata cggtactatt cttgcagaat cttggatttg gggaaatgta tgacagaacc
     2941 atcaggataa tgaaacacag gggatcaaaa catggtgaag gaatatatcc ttattccata
     3001 gaaacaggat tgggattagt tctgagagct tcgaaaaaac agatcgaccg ggttgtgcca
     3061 aaaaccaaat tcgatgagag atttgtacag gctcttgata aaacaaaatc catgggagaa
     3121 ctgggtaaaa aattggcggt aagaatcgaa gccctgagga aaaactgggt acatgatgat
     3181 gacccgcagc atattcttga aatggtcctt aaacaagaga aaaaaggcaa tgataattaa
     3241 aattgaagat actctggatc aactttatga aatcgaggga attgaagcct gcatgcttta
     3301 caggatagat ggggtgccaa taaccttaag ggccccaggt tacagggagg accttctgga
     3361 aacc
//
LOCUS       DATGBN010000066         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_509522, whole genome shotgun sequence.
ACCESSION   DATGBN010000066 DATGBN010000000
VERSION     DATGBN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509522"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /gene="ilvE"
                     /locus_tag="VKL21_01405"
     CDS             <1..761
                     /gene="ilvE"
                     /locus_tag="VKL21_01405"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HMB44456.1"
                     /translation="YNGRVFRLDEHLDRMYDSAKAIDLNIPISKDEMKKAIIETLMKN
                     NLKDAYIRPIVSRGDGDLGLDPRKCPTPNIFIITQEWGAMYGDLYEKGLTAITVGVRR
                     NAPEAMPPNIKSLNYLNNILAKIEANAKGGDEAIMFDVNGNLSEGSGDNIFVVKNGQI
                     ITPPALNNLRGITRAAAIEIAIRQGIKVAEINMGLFDIYTADEVFVTGTAAEIAPISK
                     IDGRLIADGKPGKITKMLMSAFKELTKTEGTLIA"
     gene            complement(912..1118)
                     /locus_tag="VKL21_01410"
     CDS             complement(912..1118)
                     /locus_tag="VKL21_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44457.1"
                     /translation="MTDRKYIIESRRYVDDDGNRTFDKWITSSNVIEIKHNEEYLVFF
                     PLEGEHAGKKHYIPFSNIHIVREL"
     gene            complement(1236..>1945)
                     /gene="cbiQ"
                     /locus_tag="VKL21_01415"
     CDS             complement(1236..>1945)
                     /gene="cbiQ"
                     /locus_tag="VKL21_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HMB44458.1"
                     /translation="VYVVALPRMETLNFTKLGILEAYLFTLMLFSRIELSHLAARFAI
                     ALPFGLGISTFQPFIKQPFIHDFTIISTLPMGFEITREGVLYGAIIFVKFLVCITSVI
                     LLSSTTSMSELVASARRLGLPKEMALLFTMMVRYLFVFWNMLGRIRTAQKTRCFEIWN
                     RNVPRKWTLEQIGYTISLLFIRSYEQGERTYQSMLCRGYSADANVYVNKKKLQITDFA
                     VLAVTFGIIVVVQVRVL"
BASE COUNT          605 a          399 c          410 g          531 t
ORIGIN      
        1 catacaacgg ccgggtattc aggcttgatg agcatcttga caggatgtac gattctgcaa
       61 aagcaattga cctgaatatc ccaatttcaa aggatgaaat gaaaaaagcc atcattgaaa
      121 cactgatgaa aaataacctg aaagacgctt atatacgccc catagtttca cgaggtgatg
      181 gcgacctggg tcttgacccc agaaaatgtc ccacacccaa tatattcata ataacacagg
      241 aatggggcgc tatgtacggg gacctctatg aaaaagggct taccgctatt accgtaggtg
      301 tcaggagaaa tgcgcctgag gcaatgcccc caaatatcaa atccctgaat tacctgaaca
      361 acatacttgc caagatcgag gcaaacgcta aaggcggcga tgaagcgatc atgtttgatg
      421 taaatggtaa tctttcggaa ggttcgggtg acaatatttt tgtggtgaag aacggccaga
      481 taatcactcc acccgcgctt aataaccttc gcggaataac aagggccgcc gcaatagaaa
      541 ttgccatcag gcaaggcatc aaggtcgcgg aaattaatat gggtttattc gatatttata
      601 cggcagatga ggtttttgta acagggacgg ctgcagagat agctcctatc tcgaaaatcg
      661 atgggcggtt gatagctgat gggaagcccg gaaagatcac aaaaatgctt atgtctgctt
      721 ttaaggaact aactaaaaca gaaggaacct tgatcgcatg aattgaaaaa aacatcatgg
      781 ccccatggtt aaaaaaaact tcgcgcttcg tcctttgaag ataactccaa agaaagcctg
      841 aagcgcaaag tcgttttttc tgtttatttt ctgtttatta agtctctggc aaaaaaaaat
      901 ataaaaaaat attataattc cctcacaatg tgaatatttg agaatggaat atagtgcttc
      961 ttccctgcat gttctccttc aagcgggaaa aaaacaagat attcctcatt atgttttatt
     1021 tcaattacat tcgagcttgt tatccatttg tcaaatgtcc tattcccatc gtcatctacg
     1081 tatcttctgg attctatgat atactttctg tccgtcatat tttaatctcc gttatgccaa
     1141 ctctgatatt attgtatttt aatcttgctg tggtagttta acctcgtgag gtcaaggggt
     1201 agagaagtcc ccttttcgaa agctggggga gaatgtcaca atactcgtac ctggacgaca
     1261 acgataattc caaatgtaac agccaatacg gcaaaatccg tgatctgcaa ttttttctta
     1321 ttcacatata cattggcatc tgcgctgtat cccctgcaaa gcatgctctg gtaagtcctc
     1381 tccccctgtt catatgatcg aataaaaagt aaactgatgg tatatcctat ttgttcaagc
     1441 gtccactttc tcgggacatt cctgttccag atttcaaaac agcgggtttt ctgcgctgtt
     1501 cttatccttc ccagcatatt ccagaaaaca aatagataac gcaccatcat tgtaaaaagc
     1561 agggccattt ctttgggaag gccaagtcgc ctggccgatg cgacaagttc gctcatggat
     1621 gtggttgaag aaagaagaat gaccgaagta atacatacaa ggaattttac aaaaatgatt
     1681 gcgccgtata acacaccttc acgtgtgatt tcaaacccca tcggaagtgt ggatatgatc
     1741 gtaaaatcat gtatgaatgg ttgttttatg aatggctgga aggtggagat gccaagaccg
     1801 aaaggaagag ctatagcaaa tcttgcggca aggtgtgata actcgattct tgaaaaaagc
     1861 atgagggtaa aaagatatgc ttccagtatc ccaagttttg tgaaattaag agtttccatg
     1921 cgcgggagag ccaccacata aaccg
//
LOCUS       DATGBN010000067         8818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_237929, whole genome shotgun sequence.
ACCESSION   DATGBN010000067 DATGBN010000000
VERSION     DATGBN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237929"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VKL21_01420"
     CDS             complement(<1..952)
                     /locus_tag="VKL21_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMB44459.1"
                     /translation="MNINESSHEALLLEIENLHAQLAEAKETLAAIRSGEVDAIVVSG
                     KKGEQVFTLKGAEQPYRILIEEMMEGAVMLASDDTILYCNCSFAGMLKTPLEKLIGTG
                     IANYIEPVAEAAFRKLLIQGRKKGTGGEITMLAGDRTSVPTHLSLNTLQMDDAQFVYL
                     VTTDLTEQKRIQDELRRARDGLEIRVEERTAELVKSNMTLQAEIIERKQIEKTLKESK
                     DNYRTLADSISDPFFALNKDLRFIYWNRASEKDTGISTNSIIGKYFFDVFPELKGTQV
                     EREYNEAIATGKTKFLENHRNLQGGAVTYHDLSVYPTANGI"
     gene            complement(958..1296)
                     /locus_tag="VKL21_01425"
     CDS             complement(958..1296)
                     /locus_tag="VKL21_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HMB44460.1"
                     /translation="MNETEITDEKEEFDKLLQKGDMQAYLLRLYIAGMTDKSARAVQN
                     IRKICDENLNGRYELEVIDIYQQPELARDAQIIAAPMLVKKLPPPLRRLIGDMTDKER
                     ILVGLDLRPK"
     gene            complement(1340..1663)
                     /gene="kaiB"
                     /locus_tag="VKL21_01430"
     CDS             complement(1340..1663)
                     /gene="kaiB"
                     /locus_tag="VKL21_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiB"
                     /protein_id="HMB44461.1"
                     /translation="MKNTAEITGPGSEKSDMYVLRLYVAGQTPKSMTAFANLKKLCEE
                     HLSGKYKIEVIDLLKNPQLARGDQILAIPTLVRKLPQPLKKIIGDLSNTERVLVGLDI
                     RPLKD"
     gene            complement(1676..3403)
                     /gene="kaiC"
                     /locus_tag="VKL21_01435"
     CDS             complement(1676..3403)
                     /gene="kaiC"
                     /locus_tag="VKL21_01435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HMB44462.1"
                     /translation="MLKENNIKTSDIFLLKCQTGIRGLDEITDGGIPEGRPTLVCGSA
                     GCGKTLFAMEFLVHGAIEYNEPGVFMSFEESSQELAQNVASLGFDLGELESTHKLLLD
                     HVSIERGEIEETGEYDLEGLFIRLNHSIDSIGAKRVVLDTIESLFGGLANEGILRAEL
                     RRLFRWLKEKGVTAIITGERGDGTLTRHGLEEYVSDCVIMLDNRVEGQISTRRLRIIK
                     YRGSAHGTNEYPFLIDENGISVLPITSIGLQHIVSSERISSGIPRLDTMLGGKGYFRG
                     SSILVSGTAGTGKTSIAANFANSACERGERCLYFAFEESPSQLTRNMRSIGVDLEPWI
                     RQKLLNIYAVRPTLYGLEMHLVKMYKLIREFKPNVVIIDPITNLIAVGSDTEVKSMLS
                     RLIDFLKADQITGLFTNLSHAGSTFEHTEFGISSLMDTWLLLRDIEIGGERNRGLYIL
                     KSRGMAHSNQIREFLLTDNGIDLVNVYLGPAGVLTGTARIAQEAQEKAGQTSRKQEIE
                     QKKREFHRRRAAIEAQIAALNSEFEEAQEEIDKLIQQEEAQEEALTDVSAKMAHLRAS
                     DDPNPKENR"
     gene            complement(3387..4985)
                     /locus_tag="VKL21_01440"
     CDS             complement(3387..4985)
                     /locus_tag="VKL21_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMB44463.1"
                     /translation="MDSPLRILMVEDSEDDALLLLRELRRGGYKPTFERVDTPEAMKA
                     ALLRDTWDVVIADYVMPRFSGLDALSLLHHSGLDLPFIIVSGKIGENIAVEAMRAGAH
                     DYFLKGNLALLIPAIERELREAKVRNERKLGEEELRRAHDELERRVVERTAELVTANE
                     ELKAEMMERKRIEETLRESQDLLQAIIHGTNDAIFVKDRDCRVIMANPATLRIVGKTA
                     EEVLGKKDTEIYADPEIGRSTMENDCRIMESGYSEIVEEIIKGPQGQQIFLSTKSPRL
                     DADGRIVGIIGVACNITERKRAENELRKAHDELEIRVQERTAQLKDSLIEKEILLREI
                     HHRVKNNMQVISGLLMLQEEFSNDEKFIEMIKESQNRIASMALIHEKLYRSENLSKIV
                     FREYIDDLVSGLFESYGFTESKIGLKVNAENISMGIDAAIPVGLIINELVSNSLKHAF
                     PEGKNGEINISMSSTGRDMIELSVQDNGIGIPDDLDFRKTQSLGLHIVNLLVENQLHG
                     EIKLNKERGTEFRIRFGEINVKRK"
     gene            complement(5000..5434)
                     /locus_tag="VKL21_01445"
     CDS             complement(5000..5434)
                     /locus_tag="VKL21_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB44464.1"
                     /translation="MIKRSILLVEDNPDDVALTLRALNKNNIANEVIVAKDGVEALDY
                     LFGEGAYAGCDATSVPAVILLDLKLPRIDGLEVLERLRKDERTKFAPVVILTSSKEEQ
                     DLVRSYKLGANSYIRKPVDFTRFSEAVRQLGLYWLVLNEPVP"
     gene            complement(5450..7072)
                     /locus_tag="VKL21_01450"
     CDS             complement(5450..7072)
                     /locus_tag="VKL21_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB44465.1"
                     /translation="MRDEVHLLPKMILEINEAKDFQSEIEVALKNICEITGWNYGDAW
                     ILSKDGSMLERIPAGYCSLGSPVKFKELSEKFTFPLGTGMPGRIWLSKKYEWVPDVSI
                     VSEIDFPRAQFAREAGFRACLGVPIIAGDEVLAVLVFLMFESREEEMRLVDFISVAAA
                     QLGSIIQRKKAEEQRRSASLYARNLIEASLDPLVTISSEGKIMDVNKATESITGVPRE
                     RLIGTDFSEYFTEPENARRVYQQVFSNGYVRDYLLSIRNTSGNIIDVLYNATVYRDEN
                     GKINGVFAAARDISQIKQAEEEIRKLNAQLERRVVERTKQLEAANKELEAFSYSVSHD
                     LRAPLRSIDGFSQALLEDYNEVLDMQGKDYLTRVRLASQRMSQLIDDMLVLSHITRRK
                     MKRSAVNLSALANMIAAELVKTQPQRQVEFNISPGLVVNGDLELLRAMLENLLGNAWK
                     FTSKHETAKIELGVMEIEGIPAYFLRDDGVGFDMAYVSKLFQAFQSLHSQTEFQGTGI
                     GLATVKRIINRHGGRIWAESEAGKGATFYFNL"
     gene            complement(7069..8547)
                     /locus_tag="VKL21_01455"
     CDS             complement(7069..8547)
                     /locus_tag="VKL21_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44466.1"
                     /translation="MNLRKSISLDEEYLKKIEPLIQKHNGNLSAVIREIIDLADYAYK
                     DPDYVKRLISGFKNKQSLTSSTLAWALKDLAGRRPDREFVNNIIGNDIYSISFLEKRL
                     NELLGEVYWDSSIKITSDDDRAPQNASFIITGKNQDMNRFIGALIAVFVSEKYDLGIS
                     STRSENNSFEMYMVRGTREWALKSTNDHFGYMEPAFSEFYKRPDFWNMVLTLYSRMNY
                     NLVVIPKQSFEEILGGKTSHKITTVIERFYGLPINQMPAEELLKRIKDIYQCTGLIEN
                     AHIDKESLILYHSFTEPESIKNLARMFVELLRLSGHAYSSVTSRNLIVLNPIPGTGKI
                     LIKMIDTMRNSDVRFEKYHSDLLKMLGMLENMPSNEELIGSLGWKFGIVIIENYQKYT
                     KISNWDEETFITYLKETSSLLKMDSTWEIINKNAIYGRISRFPWAENANRINNLNYTF
                     VKGMFQGWVSHAFGEETEIVFTMPSKYPGNDFSEIYIDLRLS"
BASE COUNT         2171 a         2168 c         1766 g         2713 t
ORIGIN      
        1 ctatgccgtt cgcagtcgga taaacgctca aatcatgata ggtaaccgct cccccctgca
       61 ggttccgatg gttctcaaga aacttggttt tgcctgtggc gatagcttcg ttgtactccc
      121 gctcgacctg ggttcctttg agttccggaa agacatcgaa aaagtacttc ccaatgatgg
      181 agttagtcga aatgccggtg tccttctcag atgcacggtt ccagtaaatg aagcgcaagt
      241 ccttattcag ggcgaagaag ggatcggaga tgctgtcggc aagtgttcgg tagttatcct
      301 tgctctcctt tagcgtcttc tcgatctgct tgcgctcgat gatttcagcc tgcaacgtca
      361 tattggattt taccagttct gccgttcgct cctctactcg tatttctaat ccatctcgcg
      421 ccctgcgcag ctcatcctgt attcgctttt gctcggtcaa atccgtggta accagataga
      481 cgaactgagc gtcatccatc tgcagggtgt taagggaaag gtgcgtcggt acagatgttc
      541 tatcaccggc cagcattgta atttctcccc cggtaccctt ctttcttccc tgaataagca
      601 atttccggaa tgctgcctca gcgactggct cgatataatt tgcaattccg gtacctatta
      661 gtttttcaag gggagttttc agcattcctg caaagctgca attgcagtaa aggatagtat
      721 catcggaagc cagcatgaca gccccctcca tcatttcctc gataaggatg cggtagggct
      781 gctctgctcc tttcagtgtg aaaacctgct cgcctttttt ccccgatacc acaatggcat
      841 ccacctcccc gctcctgatt gcggccaatg tttctttggc ttccgcaagc tgtgcatgca
      901 ggttctcaat ttcgagcaga agtgcttcat gcgaactttc gtttatattc ataactttta
      961 tttgggcctc agatccaatc ccacaagaat acgctcttta tctgtcatat ctcctataag
     1021 cctgcgcaga ggaggaggaa gtttcttgac aagcataggc gctgcgatta tttgagcatc
     1081 acgagcaagc tcaggctgct gatagatatc gatcacctca agttcgtatc ttccattgag
     1141 gttttcatcg catatttttc ttatgttctg tacagcccgt gcagatttat ccgtcatccc
     1201 cgcaatatat aaacgtagaa gatatgcctg catgtctccc ttttgaagta acttgtcgaa
     1261 ctcttccttt tcgtctgtta tttctgtttc attcattttt ttcccctttt ccctatagta
     1321 atattttttc tcctcctact taatctttga gggggcggat atcaagcccc acaagaacac
     1381 gttctgtatt ggagagatcg cctatgattt tctttaaagg ctggggaagt ttccttacaa
     1441 gagtaggaat tgctagtatc tggtctcccc tggcaagctg aggatttttc agaagatcga
     1501 tcacttcgat cttatattta ccagaaagat gttcttcaca gagcttctta aggttggcaa
     1561 atgcggtcat ggatttgggc gtctgtcctg caacatatag cctcaggaca tacatatccg
     1621 atttttcgga ccccggcccc gttatctctg ctgtgttttt catgtttttt cctcatcacc
     1681 ggttctcttt tggattggga tcgtctgatg cacgaaggtg cgccattttt gcgctcacat
     1741 cagtaagggc ttcttcctgc gcttcttctt gctggatgag cttatcgatt tcctcctgcg
     1801 cctcttcaaa ctcagaattc agggctgcga tctgggcttc tatggctgca cgcctgcgat
     1861 ggaactcacg cttcttttgt tcaatttcct gtttgcgcga agtttgcccg gctttctcct
     1921 gtgcttcctg cgcgatacgc gcagtgcctg tcaatacgcc tgccggacca agatatacat
     1981 tgaccaggtc tatgccatta tctgtcaaca ggaactcacg tatctggtta gaatgtgcca
     2041 tgccacgaga ctttaatatg tacagcccgc gattgcgttc acctccgatc tcaatatcgc
     2101 gcagcaggag ccatgtatcc atcaaagatg agatgccaaa ctctgtatgt tcaaatgtgc
     2161 ttccggcgtg tgacaggttt gtaaataagc ctgttatctg gtccgctttt agaaaatcaa
     2221 tgagacgcga cagcattgat ttgacctccg tatcactgcc tacagctatt agatttgtga
     2281 tcggatcgat gataacgacg ttgggtttga attcccgtat tagtttgtac atctttacga
     2341 gatgcatttc aagaccataa agtgtggggc gcactgcgta gatattcagg agcttctgcc
     2401 ttatccatgg ctcaaggtca acgccgatag aacgcatatt acgcgtaagc tggcttggtg
     2461 attcctcaaa tgcaaaataa agacatcgct caccgcgttc gcaggccgaa ttggcaaaat
     2521 tggctgcaat gcttgttttc cctgttcctg ctgtgcctga gacaaggata ctgctgccgc
     2581 gaaaatatcc ttttcctcca agcatggtat caagacgggg aatgccagag gatatccgtt
     2641 cactggatac tatatgctgc aatccgattg aagtaatggg aagcactgaa atcccatttt
     2701 cgtcgatcag gaacggatac tcatttgtgc cgtgcgctga gcctctatat ttgattatgc
     2761 gcaaacggcg tgtggatatc tgcccttcca ccctgttgtc aagcatgata acacaatctg
     2821 agacatactc ttcaagcccg tgccgcgtca gggtgccatc tcctctttct cctgtaatga
     2881 tcgcagtaac gcctttttct ttcagccatc ggaataacct tcgaagttcg gcacgcagga
     2941 tgccttcatt ggccaatccc ccaaagagcg attctatggt atccagcact acgcgtttag
     3001 ccccgatcga atcgatggaa tgattgaggc ggatgaacaa tccctcgagg tcatattcac
     3061 ctgtctcttc tatttcacct cgctcgatgc tgacatgatc aagaagcagc ttatgggtcg
     3121 attcaagttc gccgaggtca aaacccaggg aagcaacatt ctgggccagt tcttgggaag
     3181 attcctcaaa agacatgaaa acacctggtt cattatattc gatagctccg tgaaccagga
     3241 attccatggc aaacagcgtt ttgccacacc ctgcgctgcc acataccagc gtggggcgtc
     3301 cttctggtat tcccccatcc gtgatctcat ccaatcccct gattcctgtc tggcatttta
     3361 gaagaaaaat atcagacgtt tttatattat tttcttttaa cattgatttc tccaaatctg
     3421 attcgaaatt cagttcctct ttctttattc agctttatct caccatgcaa ttggttttct
     3481 accaataagt taacgatatg cagacccaga gattgggttt ttctaaaatc caggtcatcc
     3541 ggaatgccaa taccgttatc ctgcactgaa agttcaatca tatctctacc cgttgaactc
     3601 atggatatat taatttcccc attttttcct tcaggaaatg catgtttcag ggaattcgaa
     3661 accagttcat tgattatcaa tccaaccggg attgcagcat caattcccat cgaaatattt
     3721 tccgcattta ccttcaaccc aattttgctt tcagtgaatc cataagactc aaacaggccg
     3781 ctcaccaggt catcaatata ttctctaaaa acaatttttg ataagttctc ggatctataa
     3841 agtttctcat gaataagggc catagaagct atcctgttct ggctttcttt tatcatttca
     3901 ataaattttt catcattact gaattcttcc tggagcatga gcaggccgga aatgacctgc
     3961 atattgttct ttacccgatg gtgtatctca cgcagcagta tttctttttc aataagtgag
     4021 tccttcagct gtgctgtccg ttcctgaaca cgtatctcta gctcatcatg ggcttttcgc
     4081 agctcattct cggcccgttt gcgttcggta atgttgcatg caacgccaat gatcccgacg
     4141 atcctgccat cagcatcaag tcgtggggac ttggtagaga ggaatatctg ctgcccctgg
     4201 ggccctttga ttatttcctc tactatttca gaataaccgg actccataat gcggcagtca
     4261 ttttccatgg tcgagcggcc aatttcagga tctgcgtaaa tttctgtgtc tttcttgcca
     4321 agaacctctt ctgcagtttt gccgacaatt ctcagggtgg cagggttggc cataatgacc
     4381 ctgcaatcac ggtcctttac aaagatcgcg tcgttggtgc catgaatgat ggcctgcaac
     4441 aagtcctggc tctcccgaag tgtctcctca atcctctttc gctccatcat ctcagccttc
     4501 aattcttcgt tggcagttac aagttctgct gtacgctcca ctacccttct ttctaattca
     4561 tcatgagccc ttcgaagttc ctcctcgccc aactttcgtt cattgcgcac ctttgcttcg
     4621 cgcagttccc gttcaattgc cgggataagc agcgccagat ttccttttaa aaaatagtca
     4681 tgcgctccag ccctcatggc ttctaccgct atattctcac cgatcttccc tgagactatt
     4741 ataaatggca ggtcaagccc gctgtggtgc aataatgaaa gcgcatcaag ccctgaaaag
     4801 cggggcatga cataatcggc tatcaccacg tcccaggtat cccttagaag cgctgctttc
     4861 atggcttcgg gagtatctac tctttcaaaa gttggcttat aaccgcccct tcgaagttct
     4921 ctcaggagca gcagtgcgtc atcttctgaa tcctcgacca ttaatatgcg aagcggtgag
     4981 tccatttaac ctcaccgcat caaggcacag gctcattcag taccagccag tacaaaccca
     5041 attggcgcac cgcttcgctg aaccgggtaa agtccacagg tttgcggatg tagctgttgg
     5101 cgcccaattt atagcttctg acaagatcct gctcctcctt agatgaagtg agaataacaa
     5161 caggagcaaa ttttgtcctc tcatctttcc tcaggcgctc cagcacttcc agaccgtcta
     5221 tccttggcag tttcaggtcc agcagtatga ctgccgggac gctggtggcg tcgcagccgg
     5281 cataagcccc ctccccgaat aaataatcca gggcttcaac tccgtctttg gcaactatca
     5341 cttcatttgc aatattgttt ttatttaatg cgcgaagtgt aagcgcaacg tcgtcggggt
     5401 tgtcttctac aagcaatata cttcttttga tcatttaatt cctcctttgt tataaattaa
     5461 agtaaaatgt tgcacctttt ccggcctcac tttctgccca tatgcgccca ccgtgcctgt
     5521 taataatacg ctttactgta gcaaggccta tacctgtgcc ctgaaactct gtttgtgaat
     5581 gaaggctctg aaaagcctgg aaaagtttgg aaacatacgc catatcgaaa cccactccgt
     5641 catcgcgcag gaagtatgca ggtataccct cgatttccat aacgcccaat tcaatttttg
     5701 cggtctcgtg ttttgatgtg aatttccatg cattcccaag cagattctca agcattgccc
     5761 ttagaagctc aagatctcca ttcactacca atccgggaga tatattgaac tctacctggc
     5821 gctgtggttg tgttttcaca agttcggcgg cgatcatgtt tgcaagcgcg ctaaggttaa
     5881 cggcagaacg tttcatctta cgacgcgtta tgtgtgacag caccagcata tcatcgatga
     5941 gctgtgacat gcgctggctt gccaaacgca cccgggtcag gtaatcttta ccctgcatat
     6001 caagcacttc attataatct tccagcagcg cctggctgaa cccatcgatg cttcgcagag
     6061 gagcgcgaag atcatgtgat actgagtagc tgaaagcttc cagttctttg ttcgccgctt
     6121 caagctgttt cgtacgttca actacccgcc gctcaagctg ggcattcagt tttctgatct
     6181 cttcttctgc ctgttttatc tggctgatat cgcgggctgc ggcaaataca ccattgatct
     6241 ttccattttc atcccgataa actgtggcat tatataatac gtcgattata tttcctgagg
     6301 tgttacgaat actgagcaga taatctctta catacccatt cgagaatacc tgctgataca
     6361 ccctcctggc attttctggt tctgtgaaat actcggagaa atcagtacct atgaggcgtt
     6421 cacgagggac ccccgttatg ctttctgtgg ctttgttcac atccatgatc ttaccttcag
     6481 agcttatggt taccagagga tcaaggcttg cttcgatcag gttccttgca tataatgaag
     6541 ccgaccggcg ctgttcttct gccttttttc tctgaattat agaacccagt tgggccgcag
     6601 ccaccgaaat gaaatcaaca agtcgcattt cctcctctct ggattcaaac atgagaaaga
     6661 caagaactgc gagtacctca tcgcctgcaa tgattggaac cccaagacat gccctgaaac
     6721 ccgcctccct ggcaaattgg gcacgtggga aatctatttc ggaaacgatt gaaacatcgg
     6781 gcacccattc atactttttt gaaagccaga tacgtcctgg catccctgtg cccagaggaa
     6841 atgtaaactt ctcgcttaat tctttgaact ttacaggact tcccaaactg caatatcccg
     6901 caggaatacg ttcaagcatt gatccgtcct tgctcaggat ccatgcatcc ccataattcc
     6961 agcctgtgat ctcacatata ttcttcaatg caacttctat ttcagattgg aaatctttgg
     7021 cttcatttat ttctaagatc atttttggca acaggtgaac ttcatctctc atgataatct
     7081 caaatcaatg taaatttcac taaaatcatt tcctggatat tttgagggca ttgtaaagac
     7141 tatttctgtc tcttcgccaa aggcatgcga tacccaaccc tggaacatcc cctttacgaa
     7201 cgtgtaatta agattattta ttctgttggc attttctgcc catggaaacc tgctgatcct
     7261 gccgtaaatc gcattcttgt ttatgatttc ccaggttgaa tccattttga ggagagagct
     7321 ggtttctttc aggtaagtaa tgaaagtttc ttcatcccag ttacttatct ttgtatattt
     7381 ttggtaattt tctattatca ctattccgaa tttccagccc aatgatccaa taagctcttc
     7441 atttgaaggc atattttcta acatacctaa cattttcaat aggtctgaat gatatttctc
     7501 aaaccgcaca tctgaattcc tcattgtatc aatcattttt ataagtatct tgccggttcc
     7561 cggtatggga tttaagacta tgaggtttct gcttgttacg gaactgtatg catgtccgct
     7621 caatctaagt aattccacaa acatacgggc aagattttta atggattcgg gttcggtaaa
     7681 tgaatggtat agtatcaacg attctttgtc gatgtgggca ttttcaatca atccggtaca
     7741 ttggtaaata tccttgatcc tttttaataa ctcctccgca ggcatctggt ttattggaag
     7801 accatagaat ctttcaataa ccgtcgttat tttatgggac gtttttccac caagaatttc
     7861 ttcaaaggac tgttttggta ttacaaccag attataattc atcctggaat aaagagtaag
     7921 taccatattc cagaagtccg gtcttttata gaactcagaa aaagcaggtt ccatataccc
     7981 gaaatgatca tttgtgcttt ttagcgccca ttccctggtc ccccttacca tatacatttc
     8041 aaaagagtta ttttcacttc ttgtgcttga tatgccaaga tcatattttt cggaaacgaa
     8101 tacggctata agagctccta tgaaccggtt catatcctga ttctttcctg ttatgataaa
     8161 tgaagcattc tgtggcgccc tgtcatcatc cgaagttatt ttgatcgaag aatcccagta
     8221 gacttctccc agaagctcgt tcaaacgctt ttcaagaaat gagatagaat aaatatcatt
     8281 cccgatgata ttattaacaa attcccgatc cgggcgcctg ccagcgagat cttttaacgc
     8341 ccaggcgaga gtcgaagagg tcaggctttg cttattctta aatcccgaaa ttagcctctt
     8401 tacgtaatcg gggtccttgt aagcataatc tgcaagatca atgatttcac gtatgaccgc
     8461 gctcagattg ccgttgtgtt tttgaatgag tggctctatt tttttaagat actcttcatc
     8521 taaagagata ctttttctga gattcatgag actatgacta agatatatta caccgataga
     8581 ttaatttttc tgtgccaaac ttatcaaaat tattaaattg tgaatttttg taataacaag
     8641 aaatatctca gtatgaaagt attatagaat tgtacataag tacataaaaa ccatacaccc
     8701 aaaggagtgg gaacttatgg gaaaggattt ttttcctttc cttcatggtt ttaagcagat
     8761 aaaaaaaaag aatatcagga gggattatga gatactcaag aggcgatgtt gtggaatt
//
LOCUS       DATGBN010000068         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_315730, whole genome shotgun sequence.
ACCESSION   DATGBN010000068 DATGBN010000000
VERSION     DATGBN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315730"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..838)
                     /locus_tag="VKL21_01460"
     CDS             complement(71..838)
                     /locus_tag="VKL21_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44467.1"
                     /translation="MGKVKTAIPGLDELIEGGYVENDVILVTGGPGTGKTTFGVQYLV
                     EGARTFNEPGVLVVMEETPSRIMRDMWRFGWDIERLVSEKKLKIIYANPFTYTIFVKT
                     MKDRSGNVIAAKKNISDILEQIQIEVEAIHAKRIFIDSITSLIISSDLSEVRYKISEF
                     IKNLEFLDCTTLMSSEIHGEPSFKVEEYLSSGVIRLHLFRMEGNRVKAIEILKMRGVK
                     HDETLHPYEIGERGIIVYPRETVMNDEMKLFDAANNK"
     gene            complement(915..1118)
                     /locus_tag="VKL21_01465"
     CDS             complement(915..1118)
                     /locus_tag="VKL21_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44468.1"
                     /translation="MAILDGYKSFFDAAEDPNLRASKKKDGMFFIFSSIEAWKEEKCR
                     EEDIPVKELVLNEFTKNRFLAHP"
     gene            1260..>1778
                     /gene="prf1"
                     /locus_tag="VKL21_01470"
     CDS             1260..>1778
                     /gene="prf1"
                     /locus_tag="VKL21_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HMB44469.1"
                     /translation="MVESDAHKKYEFKRTLEALRDKRGRGTELISLYIPHDKQVSDIT
                     SQLREEFGQASNIKSRVTRQNVQGAIESLLSRLKLIPKAPVNGVVIFCGAVDIGANKT
                     DMQTYIIEPPEPIVSYKYHCDSSFLLTPLEDMLHEQKTYGLLVLDRREATVGILKGKH
                     IEEMAHLTSNVPG"
BASE COUNT          515 a          402 c          309 g          552 t
ORIGIN      
        1 ggaatatata tttttcgtat ttcaggacga tttcatcata attgagggcc atcttattcg
       61 attctccaga tcatttattg tttgctgcat caaaaagttt catttcatca ttcatcacgg
      121 tctcacgtgg atagacgata atgcctcttt caccgatctc ataaggatgc agggtttcat
      181 catgcttgac gcctctcatc tttaatattt cgattgcctt taccctgttc ccctccatcc
      241 tgaataaatg gagccttatg acgccagaag aaagatactc ttcaactttg aatgatggct
      301 ctccatgtat ctcacttgac atcagtgtcg tacaatcaag aaattcaaga ttcttaataa
      361 actccgaaat cttatatctg acttctgaca ggtcagaaga tattattagg gatgttattg
      421 aatcgataaa tatccttttt gcatggatag cttcaacttc tatttgtatt tgctctaaga
      481 tatcgcttat attttttttt gcagcgataa cgttgcctga cctgtctttc atcgttttga
      541 caaatatagt gtatgtaaaa ggattggcat aaattatttt cagcttcttc tcggaaacca
      601 gtctctcaat atcccagcca aatctccaca tatccctcat tatccttgaa ggtgtctcct
      661 ccattactac aagcaccccg ggctcattaa aagttcttgc tccttcgaca agatattgga
      721 ccccaaatgt ggtttttccg gttccaggac ctcctgtcac tagaataaca tcattctcga
      781 cataaccccc ttcgatcaat tcatcaagtc cagggatagc agtttttact ttacccattc
      841 atttcctcgc tgtttacgac aatttaacaa tatgtatatt tgtatatttg actgtaatga
      901 taaaacaaaa gatatcatgg atgtgcaaga aatctatttt ttgtaaactc atttaaaaca
      961 agttctttta cagggatatc ctcttccctg catttttcct ctttccatgc ttctatcgaa
     1021 ctaaaaataa agaacatgcc atcctttttc tttgaagctc taagattcgg gtcctctgcg
     1081 gcatcgaaaa atgatttata tccgtctaat atggccattc ttacacaata atgtattaat
     1141 caaatatgaa tcttactcct gttggttccc aaatattctc caaataccat gactcaacat
     1201 ttatctatga tagcagatat tgaagaacca catcttccaa tcgcagggaa ggagcttaaa
     1261 tggtcgaatc agacgcgcat aaaaaatatg aattcaaaag gacacttgag gcactccgcg
     1321 ataaacgcgg caggggtacc gaacttatct cgctttatat cccgcatgac aaacaggttt
     1381 cggatatcac aagccagctc agggaagaat tcgggcaggc ctcaaacatt aagtccaggg
     1441 tcacacggca gaatgtgcag ggtgccattg aatcgctttt atcaagacta aaacttatac
     1501 caaaggcgcc tgtgaacggt gtagtcattt tttgcggcgc cgtggacata ggcgcgaata
     1561 agaccgatat gcaaacctac ataatagagc cgccagaacc tatagtttca tacaaatatc
     1621 actgtgactc atctttcctt ctcacgccgc ttgaggatat gctgcacgag caaaagacct
     1681 atggcctcct ggttcttgac aggcgtgaag ccacagtagg catattgaaa ggaaagcata
     1741 tcgaagagat ggcgcatctt acttccaatg ttccgggc
//
LOCUS       DATGBN010000069         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_470987, whole genome shotgun sequence.
ACCESSION   DATGBN010000069 DATGBN010000000
VERSION     DATGBN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470987"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..486
                     /gene="gcvH"
                     /locus_tag="VKL21_01475"
     CDS             85..486
                     /gene="gcvH"
                     /locus_tag="VKL21_01475"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMB44470.1"
                     /translation="MNAGKTIVKDGLFYTEEHEWARIEGDIAVIGITDHAQKELRNVV
                     FVELPVTGDKLIAHKDFGYVESTKAVNSILSPVSGEVIEVNSELEESPELVNTDPYGE
                     GWMVKVRITNKDELKQLLDAKGYSELMEKLA"
     gene            499..1836
                     /gene="gcvPA"
                     /locus_tag="VKL21_01480"
     CDS             499..1836
                     /gene="gcvPA"
                     /locus_tag="VKL21_01480"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HMB44471.1"
                     /translation="MPYIPHTETEIKEMLQKIGVASIDELFADVPKETWYIEKEPFPG
                     KSEREVVHDVQSILGQNKTYPMFLGAGCYNHYVPAAVKAIISRGEFFTSYTQYQPELS
                     QGFLRALFEYQTFICQLTGMDVSNASLYDWASSLGEAALMAARIKKEKSDIIIPKTTH
                     PARVQVLKTYVKGAKLNIIEAGYDPGTGWIDIEKLKDSVNDKTAMVYMESPNFFGVID
                     ETAQNVSKIAHEAGSLFVFGTDMLSLSVLKPPDDFGADIVIGDAQSLGNPVSFGGSQL
                     GVIACKKEYVRDMPGRLVGETHDKEGRKGYVLTLQTREQHIRRQKATSNITTNHALHA
                     LAASVYIAWMGAGGFSELGSYLVKKPKEVAEKINNVKGWRAPLFYSHHFREFVAVSDD
                     DPEWINKKLLEHGITGGFSLKKWYPELGNAALYCVTECCSDEDIERLVGLLDI"
     gene            complement(1861..3351)
                     /locus_tag="VKL21_01485"
     CDS             complement(1861..3351)
                     /locus_tag="VKL21_01485"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMB44472.1"
                     /translation="MKISDLIKKFPEEKDLKAHSRPYFNHSIEFKNIFTPLERAEMLD
                     IAGLNMFFFPSEMVTGCDLLSDSGTTSMTNEQWAALHLGDEAYGSNRGYFLVMKQIRE
                     MFGEEFFNDPNSGRPNAFIFHQGRPAEDALFTMVGRLGTDLIIPGNGHFDTTGANIKR
                     NNMQAVNLFSPELLSESSGSHFKGNMDVGRFRRLLEGSSEKIPLSYLTITNNTGGGQP
                     VSMENISEVSKISHDYDIPLFFDACRFAENAWFIQQYEKGYRNKEIRTIVKEMFSYVD
                     GFTISFKKDGLVNMGGGLFLKDTGLFVKKYPDIPDALMGYQIDKEGHPTYGGLSGRDI
                     MTLAMGLKIAAKEEYLTYRINQVKDFGEDLHNHGLPVITPIGGHAVYLDMNKFFSDTE
                     MKPEDFGGVGFTAILLAAYGHRAIELGNFSFGYYDKKIQKETHPEFNFIRFAIPRLRY
                     EKKDLDSVAEAVKILYENRDKIPAVNVTYGRDLPMRHFKARFEFRK"
     gene            complement(3393..>3866)
                     /gene="amrB"
                     /locus_tag="VKL21_01490"
     CDS             complement(3393..>3866)
                     /gene="amrB"
                     /locus_tag="VKL21_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HMB44473.1"
                     /translation="IINTDETAHKYEHSIEVQLPFLQHLFSDFKIVPVCMGMQDEETA
                     LDVGMELAEAVRKVNKKVVIIASSDFSHYKPDKVAREDDAYFIRSILDLDIPGFYRKL
                     YERNASVCGYGPIAAMLTASKALGAKKGTLVKYSTSGDITGDLAAVVGYAGIIVE"
BASE COUNT         1123 a          810 c          826 g         1107 t
ORIGIN      
        1 catcgatact aaaattggaa taattatcag gaacaagata tattatggaa aggtcgttaa
       61 aacacctttc ctggaggtaa gaagatgaat gcaggtaaaa cgattgtcaa ggatggactt
      121 ttttatacgg aagagcacga atgggcacgg attgaaggtg atattgctgt aatcggtatt
      181 accgatcacg cccagaaaga attaagaaat gttgtgtttg tggaacttcc tgtaaccggg
      241 gataaattga tcgctcataa ggattttggt tatgttgaat ctacaaaagc tgtaaattct
      301 atactttcac ctgtgagcgg tgaagtaatt gaggttaata gcgaacttga agaaagccct
      361 gaacttgtca ataccgaccc atatggggag ggctggatgg ttaaagtcag gattacaaat
      421 aaagatgaat taaagcaatt gctcgatgca aaaggatatt cagaactcat ggaaaaatta
      481 gcttgatagg gaagcatcat gccttatatc cctcatacag aaactgagat aaaagagatg
      541 ttacaaaaga taggtgtggc atccattgat gagctttttg cagatgttcc gaaagaaaca
      601 tggtatatcg aaaaagaacc ttttccgggg aaatccgaac gggaagtcgt acacgatgtc
      661 cagtcgatcc tggggcagaa taaaacatat ccaatgtttt taggagcagg ctgctataac
      721 cattatgttc ctgcggctgt aaaagcaatt atttcaagag gcgagttttt cacttcatat
      781 acccagtacc agcctgaatt gagccaggga tttttgcgcg cactttttga ataccagact
      841 ttcatttgcc agctcactgg aatggatgta tccaatgcat ccctgtatga ctgggcaagt
      901 tcattgggtg aagctgcttt gatggcggcg cgtatcaaga aagaaaagag tgacatcatc
      961 atccccaaaa ccacgcatcc tgcgagggtg caggtattga aaacctacgt aaagggcgcc
     1021 aaactgaaca tcatcgaggc aggatatgat cccggaaccg gatggattga tattgaaaaa
     1081 ctgaaggatt cggtgaatga taaaactgcc atggtctaca tggaaagccc taactttttc
     1141 ggtgtgatcg atgaaacagc gcaaaatgtc tcaaagatag ctcatgaagc aggaagcttg
     1201 ttcgtattcg ggacagacat gctttcactg agcgtattga aacctcccga cgattttggc
     1261 gcagatatcg taatagggga tgcccaatcg cttggcaatc cggtaagctt tggcggatcg
     1321 cagctgggtg tgatagcatg caaaaaagaa tatgtacgcg acatgccagg acggcttgtg
     1381 ggtgagacgc atgataagga aggcaggaag ggatatgtcc tgacgctcca gacaagagaa
     1441 caacatatca ggaggcaaaa agccacttcc aatatcacga caaaccatgc attgcatgcc
     1501 cttgccgcat ctgtctatat agcgtggatg ggagcaggcg ggttttcaga actcggatca
     1561 tacctggtta aaaagccaaa agaagttgca gaaaagatca ataatgtcaa aggatggaga
     1621 gcgccgctat tttattcgca tcatttcagg gagttcgttg cagtgtctga tgatgatcct
     1681 gaatggataa ataaaaagct ccttgaacat ggcataaccg gtggattttc actcaagaaa
     1741 tggtatcccg aacttggaaa tgctgcgctt tactgtgtaa ctgaatgctg tagtgatgag
     1801 gatattgaaa gattggtggg tctgcttgat atctgaggtt acctgctatg agggcatgaa
     1861 ttatttcctg aattcgaacc tggccttaaa atgcctcatt ggaagatccc ttccataagt
     1921 gacatttact gcagggatct tatccctgtt ttcatacagg atctttactg cctctgccac
     1981 tgaatcaagg tctttcttct catatcttag cctggggatg gcaaatctta taaaattaaa
     2041 ctcagggtgc gtttcttttt gtatcttctt atcataatat ccaaatgaaa aattcccaag
     2101 ttcaattgcc ctgtggccat aagcggccag aagaatcgca gtaaacccca caccgccgaa
     2161 atcttcaggt ttcatctcag tatctgaaaa aaatttattc atatcaagat aaaccgcatg
     2221 accgcctatt ggagtgatca cgggtaatcc atggttatgg agatcctctc caaaatcctt
     2281 aacctggttt atcctgtatg tcaggtattc ttctttcgca gcaatcttta aacccatagc
     2341 aagcgtcata atgtccctgc ccgataatcc gccgtaggta gggtgcccct ctttatcaat
     2401 ttgatatccc atcagtgcat ccgggatatc aggatatttc ttcacgaaaa gtcctgtatc
     2461 ttttaagaaa agtcccccgc ccatatttac aagcccgtct tttttgaaac ttatcgtgaa
     2521 gccatccaca tatgaaaaca tctcttttac gatcgttctt atttctttat tcctgtaccc
     2581 tttttcatat tgctgtatga accaggcatt ttccgcaaat ctgcaggcat caaaaaagag
     2641 ggggatatca taatcatgtg aaattttcga aacttcactg atattttcca tagaaacagg
     2701 ctggccgccg ccagtgttgt ttgttattgt cagatatgaa agaggtattt tctcgctgga
     2761 tccttccaga agtctcctga atcttccaac atccatattt cctttgaaat gagagcctga
     2821 actctcactt aagagttccg gtgaaaaaag attcactgct tgcatgttat ttcgttttat
     2881 atttgccccg gtcgtatcaa aatgcccatt gccgggaatt ataaggtccg tacccagccg
     2941 tcctaccatg gtaaaaaggg catcttcggc tggtcttccc tggtgaaaaa taaaagcatt
     3001 tggtcttcct gagttaggat cattgaagaa ctcttccccg aacatttccc ggatctgctt
     3061 cattaccagg aaatatcctc tatttgaacc gtaggcttca tctccaagat gcagggctgc
     3121 ccattgttca tttgtcatcg aggtggtccc tgaatctgat aaaagatcgc atcctgtgac
     3181 catctctgaa gggaaaaaga acatattcag acctgctatg tcgagcattt cggctcgttc
     3241 caggggtgta aagatgtttt tgaattctat tgaatggtta aaatacggtc ttgaatgtgc
     3301 tttaaggtct ttttcttcag ggaatttttt tattaagtct gatattttca taggttgatc
     3361 tcttccttaa aagatatttc cgataattca ccctattcta caataatccc tgcatatcca
     3421 acaactgctg ccagatctcc cgtaatatct ccgctcgtac tatatttgac caacgttcct
     3481 ttctttgccc caagcgcctt tgaggcagta agcattgctg caattggtcc gtatccgcac
     3541 acagaggcat tgcgttcata caatttccga taaaaaccgg gaatatccag atcaagtatc
     3601 gagcggataa aatatgcatc atcctcgcgc gctaccttat ccggtttata atgggaaaaa
     3661 tcacttgaag cgattatcac gactttctta ttcactttgc gtacagcctc ggcaagttcc
     3721 atacccacat cgagagccgt ttcttcatcc tgcatcccca tgcagacagg aactattttg
     3781 aaatcactga aaaggtgctg taaaaaagga agctgcactt caattgaatg ctcgtattta
     3841 tgagctgttt catcagtatt aattat
//
LOCUS       DATGBN010000070         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_471007, whole genome shotgun sequence.
ACCESSION   DATGBN010000070 DATGBN010000000
VERSION     DATGBN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471007"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..221
                     /locus_tag="VKL21_01495"
     CDS             63..221
                     /locus_tag="VKL21_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44474.1"
                     /translation="MARTYPVACSGVRQRNFDSGNETLNLNQDDDEIAATQARLSGTR
                     TKINYTAR"
     gene            complement(226..549)
                     /locus_tag="VKL21_01500"
     CDS             complement(226..549)
                     /locus_tag="VKL21_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44475.1"
                     /translation="MEKREDHEAQLKQHIYDLWMRCEPQNKIAEVVDISPQRVNEIIA
                     EKSGFGQVAETGFFRDFEQENSALRIYDLWLRCETHEKIAEITGYDRTTITKIIQNKK
                     RKEGK"
     gene            976..1212
                     /locus_tag="VKL21_01505"
     CDS             976..1212
                     /locus_tag="VKL21_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44476.1"
                     /translation="MDEEQSEEHSSGVCGCGLPHDMMEGLTAEQKKKLAAMHIDIKIL
                     FFEKKIKDMESAIELKKKIIASMKQVQEIMKQSK"
     gene            complement(1981..>2243)
                     /locus_tag="VKL21_01510"
     CDS             complement(1981..>2243)
                     /locus_tag="VKL21_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44477.1"
                     /translation="IIRGFEKLLIEEESTNTKEDSKEPNNGVEIELRARVESLEGQLK
                     VKDSQISELTATMQAQAVQVQTMIQQKAIEAPGAKKRWWRFW"
BASE COUNT          656 a          417 c          378 g          792 t
ORIGIN      
        1 ttaacttttg ctcgaattaa atcgtattag ggttattgtt tccttttacg gagtttggag
       61 cggtggcgcg aacatacccc gttgcttgca gcggggtgcg ccagcgcaac tttgattcag
      121 gaaatgaaac ccttaatcta aatcaggatg atgacgaaat agcagcaacg caggctcggc
      181 tttcagggac ccgtacaaaa attaactata cggcgagata ataactcatt ttccctcctt
      241 tcttttttta ttttgaatta ttttcgtgat ggtagtgcga tcataccccg tgatttcagc
      301 aatcttttcg tgcgtttcac atctaagcca caaatcataa attcgtagtg ctgagttctc
      361 ttgttcaaaa tcgcggaaaa atccggtttc agccacttgt ccgaaaccgg atttttccgc
      421 aattatctca tttactcgtt gtggagaaat atcaactact tctgctattt tgttctgtgg
      481 ctcacacctc atccataaat cataaatatg ctgttttaac tgtgcttcgt gatcttctct
      541 cttttccaac aatttagatc aagttatcta ttttctcctt ttacattacc aaaattgcca
      601 ccatttcttt tcagctcctg gcgcttctat ggccttttga ttctgtaagt tgattcaatt
      661 cttttactcc ttctttttcg aaacaatatt cagaacaatt tcttctatac gttttataga
      721 acagaagatc gtagttagat tgtaaatata aacctccttt catgaatgag caggaagctt
      781 tgaggggagc ttcctgcaat ttaaaatctg agactttcta ttattttctg ggttttgatt
      841 gattgcagtc aattgctagc tgaatcaatg gtaaagtttg gcgattccag agaggtcaaa
      901 gtctctaaaa ataatcaatt gtattacaga cattatcaaa attttggttt agtataaata
      961 aaaggagata gttacatgga tgaagaacaa tcagaagaac actcttcagg ggtttgtggg
     1021 tgtggtttac cccatgacat gatggaaggt ctaaccgcag agcagaaaaa gaaacttgca
     1081 gcaatgcaca tagacattaa aatattattc tttgaaaaga agataaaaga tatggaaagt
     1141 gcaattgaat tgaagaaaaa gataatagct agcatgaaac aagttcagga aataatgaaa
     1201 caaagtaagt gaggctatcg ccatacgcta gttgtctaat tatgcaactg tgctacgaat
     1261 tagaggcttt ttaatacttt ctagattacc ttgactatgt cacattacaa ggtaaatcgt
     1321 catcattaga tctcgtatga caaagatatt gttcgtagtg aaatcgataa cagcgattgg
     1381 agtaactgtc taatctgaca aagtcaattt acaaaactta gaattaattc actgatattt
     1441 agctactcta aataaaaaca ctgaaagtaa tgaaaagtct ctaaaatctt ctttttctaa
     1501 ttgctgtttc ctgataacat acttccaata cattaccaaa atttgtacca gggtttttta
     1561 gttccttgct tcaatcaatt tctgattgct gagagtttgc acctacatag catgttttca
     1621 ttagttcagt taatatttct ccataatccc taatatcaga atttacaaag taaaatagca
     1681 tttccatcgt attatctttt gtagggtcat cgcaacatct tttaaatgaa tctgcggtat
     1741 gtagtgttgc atacctaaat gctagatttt cgaatttttg aagactttat ttattaatga
     1801 tggtaccgtt agccatacat tgctctatct cagttttaag cggataatag aaataatcta
     1861 gagtttcctt tatttctttt ctatgcataa aatataatta acggtgacat atatataaat
     1921 attgtactgt ttcagttggt attttatttc actgcaatca cgtagatcca aaagttctag
     1981 ttaccaaaat ctccaccatc tcttttttgc tcctggtgct tctatagctt tttgctggat
     2041 cattgtttgt acctgtaccg cttgggcttg cattgtagcg gtaagttcgc ttatttgact
     2101 atcctttact tttaattgcc cttctaaact ctctacccgt gccctcagtt caatctcaac
     2161 tccgttgtta ggttcttttg agtcttcctt tgtgtttgta ctttcctctt caatcagaag
     2221 cttctcgaag cctcgtataa tag
//
LOCUS       DATGBN010000071         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_160366, whole genome shotgun sequence.
ACCESSION   DATGBN010000071 DATGBN010000000
VERSION     DATGBN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160366"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1395
                     /locus_tag="VKL21_01515"
     CDS             280..1395
                     /locus_tag="VKL21_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMB44478.1"
                     /translation="MRTVRKVLVLGNSVRNIVCSARKAGYIVYALDRFGDVDMRKCAN
                     KAQILENIPCNDLHKIVESFGDFDAIIPGPGFENPKFKNTLSNPLKIIEHAGDKSKLP
                     GKMNSMGIPHPRTESVDKADGMGFPLMVKPKAGSGGMKNIIIRNETEMKNFQTRKDSH
                     DFIAQEFVKGIPCSASLISTGDDALVVAINEQLIGTPWLTRIPFAYCGNITPFHTKFK
                     AEMVQYALQIAMEFKLKGSNGVDFMLTDKGVQVLEINPRFQGSLDTVELSTGMSIFDA
                     HIRSFEGELPELRGFHCFAAKTIIYAEKELMITREISDTLIKCMNKGRGADIPTKGVV
                     IQPDEPITTMLSTARARMTALDKVRKSSQHIKRRTEA"
BASE COUNT          507 a          273 c          332 g          388 t
ORIGIN      
        1 cttggactag tgacctatac acgaaaaaag aaggcacctg aaaaagagat atgggcaaca
       61 ggttgacaag gcataaatta tgcagaagtt agaggaaata aatacataca cacccacaga
      121 tattccgcac gttagtggca actgtgaaca cgcaaagcag aacacgaaag aactcacatt
      181 attagaaatt gctaatacgt ttttaatagc acctttgaag cggggtacgc tactccacac
      241 tttgaaaagt atggtttccg ctacccccta tggagtatta tgagaacggt caggaaagta
      301 cttgtgttgg gcaacagtgt ccgaaatatc gtctgttctg caagaaaagc aggatatatt
      361 gtttatgctc ttgaccgttt tggggatgtg gatatgcgaa aatgtgcaaa taaggcccaa
      421 attcttgaaa atattccctg taatgatttg cataaaattg tggaatcctt cggggatttt
      481 gatgcaataa tccctggacc tggttttgaa aatccgaaat ttaaaaatac tttgagtaac
      541 cccttgaaaa tcatagaaca tgcaggcgat aaatcaaagc ttcccgggaa aatgaactcg
      601 atgggtatac cccaccccag aaccgaatcg gtggataaag cggatggtat gggattccct
      661 cttatggtca aaccaaaggc aggatcgggg ggaatgaaaa atatcattat ccggaatgaa
      721 actgaaatga aaaattttca aacccgaaaa gactcacacg atttcatagc ccaggagttc
      781 gtgaaaggaa taccatgcag cgcctccttg ataagtaccg gggatgacgc cttagttgtt
      841 gctataaacg aacagctaat aggcacccca tggctgacaa gaataccatt tgcatattgc
      901 ggtaatatca ctccttttca tacaaaattc aaagcagaaa tggtgcaata tgcattgcaa
      961 atcgccatgg aattcaagtt aaagggttct aatggtgtgg atttcatgct gaccgataaa
     1021 ggcgtacagg tccttgaaat aaatccgcgt ttccagggaa gtcttgatac tgttgaactt
     1081 tcaaccggaa tgagcatttt tgatgcgcat ataagatctt ttgaagggga acttccggaa
     1141 ttaagaggat ttcattgttt tgctgcaaag accattattt atgctgaaaa agaattgatg
     1201 attacccggg agatctcaga tactcttatt aaatgtatga ataaaggaag aggggccgat
     1261 attcccacaa agggagtggt aatccagcct gatgagccaa taacaacaat gctttcaacc
     1321 gcccgggcac gcatgacggc tctggataaa gttagaaaat cctcacagca tattaaaagg
     1381 agaacggaag cttaaagtca tataaaaaca atataaatat ggtgaatttt ctggttgatt
     1441 tgaacgattc cgttattaaa ggttatctaa taaatcttgt aggtgaggaa gggtataaag
//
LOCUS       DATGBN010000072         5402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_510039, whole genome shotgun sequence.
ACCESSION   DATGBN010000072 DATGBN010000000
VERSION     DATGBN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510039"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VKL21_01520"
     CDS             <1..193
                     /locus_tag="VKL21_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HMB44479.1"
                     /translation="KIAGRTGYITFSRVKIDKGRAIPLATMGAGILSSVSKADGFVII
                     PENIEGRNAGEEVEVVLIE"
     gene            281..568
                     /locus_tag="VKL21_01525"
     CDS             281..568
                     /locus_tag="VKL21_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44480.1"
                     /translation="MIRNIRKIGNSRGIIIPRDILQELGYPKTVEITLTKGGIFINPI
                     AGKAIRRKPRNQDETDGFYDLMKSKLESNLAIGKTRWIGNREMERRIELKN"
     gene            625..1095
                     /locus_tag="VKL21_01530"
     CDS             625..1095
                     /locus_tag="VKL21_01530"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HMB44481.1"
                     /translation="MTQEAEKRKLKPIVVGGSAVDFYTEGIYSSYDIDLVSNKREIIG
                     EILENLFNFKPSGRHWINEQIGLSVEIPDNNLAGDTDKVTVIRIKNLKVYVIGLEDLI
                     IDRLNACVYWKSQRDCDQAKYMIKYYRNRLDFGYLERKARVEGILKILREFYKE"
     gene            complement(1140..1224)
                     /locus_tag="VKL21_01535"
     tRNA            complement(1140..1224)
                     /locus_tag="VKL21_01535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1186..1188),aa:Leu,seq:tag)
     gene            complement(1489..1677)
                     /locus_tag="VKL21_01540"
     CDS             complement(1489..1677)
                     /locus_tag="VKL21_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44482.1"
                     /translation="MSDKNQTSSKHQKLSGEKKKKKSGWISRNSSTKDRPKKDDGVVY
                     TRGQTYEEYLASLETPEE"
     gene            1922..2359
                     /locus_tag="VKL21_01545"
     CDS             1922..2359
                     /locus_tag="VKL21_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMB44483.1"
                     /translation="MKGIYVLILRLDTDLDITIGKLGIINFKSGYYYYIGSALGTGGF
                     KRVTRHFNVASGKNLTRKWHIDYLLPHSDVICALLLPTDEALECRVAEKLKEYSDIIP
                     GFGCSDCTCKTHLFFSDMDIRKVLIDTANKLTGNESIIIYPSM"
     gene            2367..3377
                     /gene="argC"
                     /locus_tag="VKL21_01550"
     CDS             2367..3377
                     /gene="argC"
                     /locus_tag="VKL21_01550"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HMB44484.1"
                     /translation="MDIGIIGGSGYTGGELMRLLSQHPEAKIKAVTSRNRKGQKISDT
                     HAHLRKIIDSEFEDLSPEEVASRCDIAFTAVPHGNAMQVVPALIDSGIKVIDMSADYR
                     LRTDVFEKNYKLKHIDPRKTVYGLPELHPDISQQQFIANPGCYPTGASLAAAPLASAG
                     LIGRAVFDSKSGISGAGIQPSEVSHYPNMAENIQAYKLTTHRHRAEIVQELTRLDGSL
                     NSISFTPHVIPSVRGILTTAHIFVKKELSSEDVSAIYSDFYRDKPFIRLIKGIPMLGN
                     VRGSNFCDIGFEVENDSDRIVVISAIDNLVKGASGQAIQNMNLMFGLPETCGLWSPGF
                     AP"
     gene            3387..3860
                     /locus_tag="VKL21_01555"
     CDS             3387..3860
                     /locus_tag="VKL21_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB44485.1"
                     /translation="MKIKDVMNKDVITCKPDDTLGTLANILKENHISGLPVVDNGKVV
                     GLVSETDLLKLFKFPEISNELWLPSPFEIIEIPIRNLVKIEETKKAFENIKLRPVKDI
                     MTNTIHAISSQDNMEDASSMMIKYQVNRLPVIDNGNLVGIVARSDILRGLSESTE"
     gene            3863..5038
                     /gene="argJ"
                     /locus_tag="VKL21_01560"
     CDS             3863..5038
                     /gene="argJ"
                     /locus_tag="VKL21_01560"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HMB44486.1"
                     /translation="MKNIKGGICAVKGVRANGIKQGKNGLAIMVADARKLATAGVFTR
                     NKVIAAPLIITEASLEKGDLKAIIANSGCANAFTGEKGLADAKWMADILAEKMKCTPN
                     EIGVASTGVIGRLLDRIWIEGHLDEVFDQLSDTEEAAKAAAKAIMTTDLAIKEIAIEL
                     DNGVHIGGISKGSGMIEPNMGTMLAFLYTDAILSKETLDSCLRKAVDKSFNMVVIDGD
                     TSTNDMALITATGYHDCDEDVFQKGLDHVCANLAKMIARDGEGASRLVEARVKGARSP
                     QDARLAAKAIVRSPLVKTAIFGKDPNWGRVVAAAGYSGADIDQDRISLKFSDNMKEVL
                     LVDSGRIVEGKLDALKSIMESKEIIIEVDIGLGDSVATAWGCDLTYDYVKINSMYTT"
     gene            5108..>5402
                     /locus_tag="VKL21_01565"
     CDS             5108..>5402
                     /locus_tag="VKL21_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMB44487.1"
                     /translation="MSLKKSGFSTRAIHSGEKHEETGSVTTPIYQTAPFKFKNAAHAA
                     DLMGGRKKGYVYSRGLNPTTEVLEEKMADLEGGEASLAQTTGMAAISAAVFTSV"
BASE COUNT         1737 a          980 c         1247 g         1438 t
ORIGIN      
        1 aaagattgcg ggaagaacag gatatattac attttcccgt gttaaaatag ataaaggcag
       61 ggccattcca cttgcaacga tgggagccgg gatcttaagc tcagtgtcta aagctgatgg
      121 ttttgtgatc attcctgaaa atatcgaagg acgaaatgca ggtgaagagg tagaagtagt
      181 attgattgaa tgataattga atgcgcagga aatggttggg tgaaaaaaag gaaaaccaca
      241 aactcttata tataaagaat gttataacat ttgttataac atgataagaa atatcagaaa
      301 aatagggaac agtcgaggaa tcataatccc aagagacatt ctacaagaat tgggatatcc
      361 taaaacagtg gaaataacat taaccaaagg tggtatattc ataaatccaa tagctggcaa
      421 agccatcaga cgtaaaccaa ggaatcagga cgaaaccgat ggattttacg atttaatgaa
      481 atcaaaatta gaaagcaatc tcgctattgg aaagactcga tggattggaa atagagaaat
      541 ggagaggaga atagaattaa agaattagaa aaaatatcat ctatagagaa tttagttgat
      601 agaaaaatat atttcatggg actgttgacg caagaagccg aaaaaagaaa gttgaaacct
      661 attgttgtgg gcggttcggc tgtggatttc tatactgaag gaatttattc aagttatgat
      721 atagaccttg taagtaataa gagagaaata attggggaaa tattagaaaa tttattcaat
      781 tttaaaccaa gtggaagaca ttggataaat gaacaaatag ggctttctgt agagattcca
      841 gacaataatc tggctggaga cactgataaa gtaacggtaa ttagaattaa gaacttaaaa
      901 gtatatgtaa ttggacttga agatttaata attgaccgtc ttaatgcatg tgtttattgg
      961 aagtctcaaa gagattgtga ccaggcaaag tatatgatta aatactatcg aaatcgatta
     1021 gattttgggt atttggaaag aaaagccagg gttgagggta ttctgaagat attaagagaa
     1081 ttttataaag aataaatctt tgtaatccta aaaacagacg aattgaactt ataattgtat
     1141 gcgagggatg ggattcgaac ccatgaactc cttcgagatt agcccctaaa gctaacgcct
     1201 ttgacctgac tgggcgaccc tcgcaatgga caaaatcatc aggctgggcg atcgatacaa
     1261 attcctggca gcaccgccaa aatgatcctt ataccataaa tacatcacta cacggttctc
     1321 tcaagtctgc gattgcttgc tgtcaaaatt cggttgctgg attgcgttac actggcatta
     1381 ttgattattg cgactcggag aataagtccg atataaatcc caaatgtctt caatcagaac
     1441 ggagtggatt ttatgttcta aatcacgatt ttttttcagg taattttatt attcttctgg
     1501 agtttccagt gaagccaaat attcttcata tgtttgtcct cttgtatata caaccccgtc
     1561 atctttcttg ggtctgtctt tagtactgct gtttcttgag atccatccac ttttcttttt
     1621 cttcttttct ccgcttaatt tctggtgttt tgaagatgtt tggtttttgt cgctcataat
     1681 ttaatttcta tactatgttt ggtttgtgaa actataaagg aatatcttga aaaagaaaaa
     1741 aatgagtaga aaggaattag acattatctt caagcaggaa tattttatct cagaatctgg
     1801 tctaaaaaac agatatagcg ccttacataa attttatact tgaataaatt aatgttgtcc
     1861 tgatattaat ttacactctc tatgtccaaa ggcgggagat tctttagttc ggtaaaacaa
     1921 catgaaagga atttatgtcc tgatcctcag attagatact gaccttgata taaccatagg
     1981 aaaacttgga ataatcaact tcaagagcgg ttattactat tacatcggtt cggcgcttgg
     2041 aacaggaggg ttcaagcgcg tcacaagaca tttcaatgtg gcttccggta aaaatctcac
     2101 caggaaatgg catattgatt atcttcttcc ccattccgat gtaatatgcg ctttactttt
     2161 accaacggat gaagctcttg aatgcagggt tgcagagaag ctaaaggagt attcagatat
     2221 tatccctggc tttggatgca gcgactgtac atgtaagacg catctttttt tcagtgatat
     2281 ggatatccgg aaagttttaa ttgatactgc caataagctg actggaaacg aaagtatcat
     2341 tatttatccc agcatgtagg aatcacatgg acataggaat aatcggaggc tccggatata
     2401 cgggcgggga attgatgagg cttctttccc agcacccgga agcaaagatc aaagctgtta
     2461 cctcgcgaaa caggaaagga cagaagatca gcgatacgca tgcccatctc aggaaaataa
     2521 tcgactccga atttgaagac ctgagtcctg aagaagtagc ttcccgctgt gatattgctt
     2581 tcactgcagt ccctcatggc aacgcaatgc aggtagtccc agctcttatt gattcaggca
     2641 tcaaggtcat cgatatgagc gctgattatc gtctcaggac agatgttttt gaaaaaaact
     2701 ataagctaaa acacatagat ccgcgcaaga cagtgtatgg gcttccggaa cttcatccgg
     2761 atatctcaca gcagcaattt attgcaaatc ccggctgcta tccaacaggc gcctcgcttg
     2821 ccgctgcgcc tttagccagt gccggactga tcgggcgcgc agtatttgat tcaaaaagtg
     2881 gaatatcggg cgcgggtatc cagcccagcg aagtatccca ttatcctaat atggcagaga
     2941 atatccaggc ttataagcta acaacacatc gccaccgcgc agaaattgtg caggaactta
     3001 cccggcttga cggcagtttg aatagtatca gctttacacc gcatgttatt ccatcagtac
     3061 ggggaattct cacaacggct catattttcg tgaagaagga actgagtagt gaagatgtca
     3121 gcgctatata tagtgatttt tacagggata aaccattcat aaggttgata aaaggcatac
     3181 ccatgctggg aaatgtacgc ggttccaatt tctgcgatat aggttttgaa gttgaaaatg
     3241 atagcgacag gatagttgtg atctctgcaa ttgacaatct ggtaaaagga gcgtcagggc
     3301 aggccatcca gaacatgaac cttatgttcg gccttcctga gacatgtggt ctctggtcgc
     3361 caggttttgc tccgtgaggt gaaaatatga aaataaaaga tgttatgaat aaagatgtga
     3421 ttacatgtaa accggatgac acactgggaa ctcttgccaa tatattaaag gaaaatcata
     3481 tcagcggctt gcccgttgtt gataatggaa aagtagtcgg gcttgtttcc gaaactgacc
     3541 ttctaaagtt attcaagttc cccgaaattt caaatgagtt atggctgccc agtccttttg
     3601 aaataattga aatccctatc aggaacctgg ttaagataga agaaacaaag aaggcctttg
     3661 aaaacattaa gctgagacct gtcaaggata taatgacgaa taccattcac gcaatttcat
     3721 cacaggacaa catggaagat gcttcaagca tgatgattaa gtatcaagtg aacaggctgc
     3781 cagtcattga taatggcaac ctggttggaa tagttgcccg cagtgacata ttaagaggtc
     3841 tttcagaatc gactgaataa atatgaaaaa catcaaaggc ggcatatgcg ccgtaaaagg
     3901 cgtaagagct aacggaataa agcaggggaa gaacggactg gctattatgg ttgcagatgc
     3961 tcgaaaactt gcaaccgctg gtgttttcac acgaaacaaa gtgatagcag cgccgcttat
     4021 tattacggaa gcatcccttg aaaaaggaga tttaaaagca ataattgcca atagtggttg
     4081 tgcgaatgca ttcacaggtg aaaagggact tgccgatgcg aaatggatgg cagatatact
     4141 ggcagaaaaa atgaaatgca caccgaatga gataggagta gcctcaactg gtgtgatcgg
     4201 gagacttctt gatcgaatat ggatcgaagg acatcttgat gaggtttttg accaattgtc
     4261 agataccgaa gaagcagcta aagccgctgc aaaagccata atgacaactg atcttgccat
     4321 aaaagagatc gctatcgagc ttgataatgg tgtgcacatc ggcggtatct cgaaaggttc
     4381 ggggatgata gaaccgaata tgggtacaat gcttgcattc ttatatacgg atgccatttt
     4441 atcaaaggaa actcttgatt catgtcttcg aaaggccgtt gataagagtt tcaatatggt
     4501 ggtaatagat ggtgatacaa gcaccaatga tatggccctg atcacagcta cgggatatca
     4561 tgattgcgat gaagatgtgt tccagaaggg gcttgaccat gtgtgcgcca atcttgcaaa
     4621 aatgatcgca agagacggtg agggagcaag cagacttgtg gaggccaggg tcaaaggcgc
     4681 acgaagcccc caggatgcgc gattggccgc caaggcaata gttcgttcac cgcttgtgaa
     4741 aactgccata ttcggcaaag acccgaactg gggaagagta gtcgcagctg caggttactc
     4801 aggcgctgat atcgaccagg acaggatatc ccttaagttc tcggataata tgaaggaggt
     4861 tttgctggtc gattcgggca ggatcgtaga ggggaagctc gatgcgctaa aaagcattat
     4921 ggaaagcaag gagatcatca ttgaagtgga tatcggtctt ggcgattccg tagcgactgc
     4981 ctggggctgc gatttgactt atgattatgt gaagatcaat tcgatgtata cgacgtaaaa
     5041 agtctcatag gtctcctggc aatatttata tcttgactga agagaaagtt aacaagggaa
     5101 ggttaaaatg agcttgaaaa aatccggatt ttccacacgc gcgatccact caggagaaaa
     5161 acatgaggag acagggtcag tcacaacccc gatataccag acagcgcctt ttaaattcaa
     5221 gaacgccgcc catgcagcag acttgatggg tgggcgcaag aaaggatatg tgtattcacg
     5281 cggcctgaac cctactacag aagttcttga ggagaaaatg gctgaccttg agggcggcga
     5341 agcctcactt gcacaaacaa caggcatggc agcgattagc gctgctgttt tcacatctgt
     5401 aa
//
LOCUS       DATGBN010000073         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_432093, whole genome shotgun sequence.
ACCESSION   DATGBN010000073 DATGBN010000000
VERSION     DATGBN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432093"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1964
                     /locus_tag="VKL21_01570"
     CDS             51..1964
                     /locus_tag="VKL21_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HMB44488.1"
                     /translation="MTVEEVLNDLKKRIKAQLPKNITISDVDFEGPELVIYTEEPRKF
                     ADDGDLIKGLAKELRKRIVVRPDPKVLAQPEEAITAISKIVSADSGISNHFFDVDTGE
                     VVIEAEKPGLVIGRHGETLREITKEIGWTPKVVRTPPMKSTTVNNIRQFMRANKEERK
                     TLLKTIGRKIHRGVSSKDKWVRLTTLGACREVGRSCFLLSTPETRILIDCGVNVSGED
                     NGTPYLYVPEVHPISQIDAIVLTHAHLDHIGLVPLLYKYGFEGPVYCTPPTRDLMALL
                     QLDYLDVAAREGKKIPYESAMIREVLKHTITMNYGDVTDIGPDMKLTLHNAGHILGSS
                     VAHFHVGDGLYNIAFTGDFKYEKTRLFDPAVNDFPRIETIIMEATYGGSRDMQPSRKD
                     AERHLQEIAKETLEKGGNLLIPTFAVGRSQEVMIVLEEAIRKGIINDVPVYLDGMIYE
                     ATAIHTTHPEYLNNELRNQIFHKGMNPFLAKCFVQVDSKEKRQKILDDRDPCIVLATS
                     GMMNGGPVMEYLKSFGPEEKNTLVFVGYQAEGTLGRRIQKGWKEIPMHAAGGRTETVK
                     INMNIDTVDGFSGHSDRMQLMEYIRKMRPRPEHVLTQHGDEKNCMDLASSIYRKFKIE
                     TRAPMNLETIRLA"
     gene            complement(2025..>2618)
                     /locus_tag="VKL21_01575"
     CDS             complement(2025..>2618)
                     /locus_tag="VKL21_01575"
                     /inference="COORDINATES: protein motif:HMM:NF021831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser-Thr-rich GPI-anchored membrane family
                     protein"
                     /protein_id="HMB44489.1"
                     /translation="VNYNTGSIATTTTYWDKTDFDKIIENDMELNDLTLDWSTDGSIT
                     RMDVQTIATHELGHFLGFADMYGTADNVKIMNGYGAEGKIKRKLTKDDISGIRGIYGS
                     KSITVKYPNGQETWVRGNAYTIKWSYKGDPGSDVRIQLLKNGSVVGVVTSSTPNDGSY
                     NWTVSSRRTPGNDYMIRVTSTSNSAYKDTSNKNLIIE"
BASE COUNT          794 a          524 c          556 g          744 t
ORIGIN      
        1 ttacataagg tcatatttta tttttagagt gttttataag gaaaacaata atgacagttg
       61 aagaagtatt aaatgatttg aaaaagagaa taaaagcaca gcttcctaaa aatatcacga
      121 tctctgatgt agattttgaa gggcctgaac ttgttattta taccgaagag ccacgtaaat
      181 tcgctgatga cggcgatctt atcaaaggtc ttgccaagga actgagaaaa cgtattgttg
      241 tcaggccaga ccccaaagtc cttgcgcagc ccgaggaagc tattacagct atttcaaaga
      301 tcgtttcagc ggactccgga atttcaaatc atttttttga cgtggatacg ggtgaggttg
      361 taattgaagc agaaaaaccc ggtcttgtca taggaagaca tggagaaaca cttcgtgaga
      421 taacaaaaga gatcggatgg acaccaaaag tggttcggac acccccgatg aaatccacga
      481 cagtaaataa tattcgccaa ttcatgcgcg ctaacaagga agaacgaaaa actcttctta
      541 agactatcgg gagaaagata caccgcgggg tttcttccaa ggataaatgg gtacggttga
      601 ccacactagg cgcatgccgt gaggttggac gcagctgttt tttgttatca actcctgaaa
      661 caaggatttt aatagactgc ggtgtaaacg taagtggtga agataatggg acaccttatc
      721 tttatgtgcc cgaagtccat cccataagcc agatagatgc aatagttctt actcatgcgc
      781 atcttgacca tatcggactg gttccattat tgtataaata tggatttgaa ggacccgtct
      841 attgcactcc tccaacgagg gatctaatgg ctttacttca gcttgattac ctcgatgtcg
      901 ccgcccgtga gggaaagaaa attccctatg agtcggctat gatacgtgaa gttctcaagc
      961 atacaatcac aatgaactat ggggatgtca cagatatcgg tcctgatatg aaattgacat
     1021 tgcataatgc aggtcatatt cttggttctt cggtcgccca tttccatgtc ggagatggcc
     1081 tatataatat tgcttttacc ggggatttca aatatgaaaa gacacgttta tttgatccgg
     1141 ctgtcaatga tttcccgcgc atagagacaa taatcatgga agcgacttat ggagggtcaa
     1201 gggatatgca gccctcaagg aaagatgctg agaggcattt gcaggaaatc gcaaaagaaa
     1261 cacttgagaa agggggaaat cttcttatac cgacatttgc tgtgggaaga agccaggaag
     1321 tcatgatagt gcttgaagaa gcaattcgaa aaggaattat caacgatgta cccgtttatc
     1381 ttgacgggat gatatatgaa gcaacagcga ttcatacaac acatcctgaa tatttgaaca
     1441 atgagctgag aaaccagata ttccataaag gtatgaatcc tttccttgca aaatgcttcg
     1501 ttcaggtaga ttcaaaagaa aaacgccaga aaatcctgga tgaccgtgat ccctgcatcg
     1561 ttcttgcaac atcgggcatg atgaacggag gtcctgttat ggaatatttg aagtcattcg
     1621 ggcctgagga aaaaaataca ttggtcttcg tgggatacca ggccgaaggt acactcggca
     1681 ggcgcatcca gaaaggctgg aaagaaattc ccatgcatgc agccggagga agaactgaaa
     1741 ccgtaaaaat caacatgaat attgatacgg tagatggatt ttcaggtcat tctgacagga
     1801 tgcagcttat ggaatatatc cggaaaatga ggccaagacc ggagcatgtc ctgacacagc
     1861 atggtgacga aaagaactgc atggaccttg ccagttcgat atatcgcaaa ttcaagattg
     1921 agacccgggc gccaatgaac cttgaaacga tcaggcttgc ctagggtttt tctcaatatt
     1981 ttaacaaaaa tcggagcagg ttgatatgga tttgctcaaa aatattattc tattattaaa
     2041 tttttgttac ttgtgtcctt ataggctgaa ttacttgtac ttgtgactct tatcatatag
     2101 tcatttccgg gggttcttct tgatgaaact gtccaattat atgaaccatc attaggcgta
     2161 ctcgaagtaa ctaccccaac taccgatcca tttttcagta attgtatcct gacatctgac
     2221 ccaggatcac ctttatagct ccattttatt gtatacgcat tgccacgtac ccaggtttcc
     2281 tggccatttg gatatttaac tgtaatcgat tttgatccat aaatccctct tatgccgcta
     2341 atatcgtctt tggtcaattt tctttttatt ttcccttcag cgccataccc gttcattatc
     2401 tttacattat cggcagttcc atacatatca gcaaacccta gaaaatgtcc cagctcatga
     2461 gttgctatag tctgaacatc catcctggtt atggatccgt cagtactcca atccagtgtc
     2521 aaatcattta attccatgtc attttcaatg attttatcaa aatctgtttt atcccaatat
     2581 gttgttgtcg ttgcaattga acctgtgtta taattgac
//
LOCUS       DATGBN010000074         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_83154, whole genome shotgun sequence.
ACCESSION   DATGBN010000074 DATGBN010000000
VERSION     DATGBN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83154"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VKL21_01580"
     CDS             complement(<1..586)
                     /locus_tag="VKL21_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44490.1"
                     /translation="MNTVESPGFSRGRGFNNNIPPRLRIEMPLGICLHIKNPEIEPGE
                     YPSCRIQKGLVLVHENRDLSEEGIGFAVPVLKFGHETIFPGNACVTTEKDGDTSVVKI
                     DYDMNLVERMVVKSSKMIDSEAFYKIKECFSWLHREYPRIRGILVRSSNSLRRTCDIE
                     TRFEKIASKGIASVVNTVNTKRDTIHVDVDISNVK"
     gene            complement(583..891)
                     /locus_tag="VKL21_01585"
     CDS             complement(583..891)
                     /locus_tag="VKL21_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44491.1"
                     /translation="MYSELIYTNTSSKHKAENAGGYLKYAISALDTARQKSCWQGLES
                     VLSDAVSRVKQTAIGKVCPANFGFNIQSQSGSHANPVTSISEAMDAVIFQDESHRELI
                     "
     gene            complement(1123..1431)
                     /locus_tag="VKL21_01590"
     CDS             complement(1123..1431)
                     /locus_tag="VKL21_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44492.1"
                     /translation="MEQIPDVLKVAAKAYKLLMENEKVRVLDIRLKPGEKTPMHNHPD
                     SHVVYVMNNTRFKLTFPDGKTGEFDLNAGQTLWLEAGSHATENIGITEGHNLVVEIKK
                     "
     gene            complement(1456..2007)
                     /locus_tag="VKL21_01595"
     CDS             complement(1456..2007)
                     /locus_tag="VKL21_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HMB44493.1"
                     /translation="MQLPKNKSNRKAVVLTADKFEDMEVFFPVFRLLEEGWQVDIAAP
                     SLKEISGEKGYVLKPDKTIEEVDPGEYDLLIIPGGAPNGAATVRKIKKAQEIAKSFFA
                     KNKPVASICHGPYTLVSAGIVKGRHLTSYWHDGVPEEIQEAGGIWEDKDVVVDGNLVT
                     SRWPMDLPAFMREVMRMVKLVEK"
     gene            complement(2264..2623)
                     /locus_tag="VKL21_01600"
     CDS             complement(2264..2623)
                     /locus_tag="VKL21_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44494.1"
                     /translation="MQARGDELFSNKAKCNNCHSEPLWTEPGWNLHKPSEIGIDSFQA
                     DRAPDGLYKTMNLGGIYIRENGKFMKPENKGRFYHDGRFKTLLDVVDHYDKLSTLNLS
                     HQEKGDVVEYLKSLSSP"
     gene            complement(2604..>3022)
                     /locus_tag="VKL21_01605"
     CDS             complement(2604..>3022)
                     /locus_tag="VKL21_01605"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HMB44495.1"
                     /translation="PDAGRHSDPGLVPYYDNINANKTAPNFTSADQPAGPYWIFDVQK
                     KPFNDTYNTGDEIAGIIVRPPTVDRADIMGKAVYMDGNWTLEYGRKLMTGSQFDVQFS
                     DMKKEYLFGTAVFDNAQTRHSYESGVSKFVFASAWG"
BASE COUNT          766 a          722 c          574 g          960 t
ORIGIN      
        1 ttttcacatt gcttatatcc acatcgacat ggattgtatc ccttttagta ttaacagtat
       61 tcacaacact tgctataccc ttagaggcga tcttttcgaa tctggtctct atatcacagg
      121 tgcgccttaa tgaattggaa gaacgaacca ggattcctct tatccgtgga tattccctgt
      181 gcagccagga gaaacattcc tttattttgt aaaaagcttc gctgtcgatc atcttgctgc
      241 ttttcaccac catcctctcc accaggttca tatcataatc gatcttgacc actgatgtat
      301 caccatcttt ttcagtcgtt acgcatgcat ttcccgggaa aatagtctca tgcccgaact
      361 tgagcacagg tactgcgaat ccaatgccct cttcagacag atctctattt tcatgaacca
      421 gcacaagccc cttttgtatc ctgcaggagg gatattctcc cggttcaatt tcaggattct
      481 taatatgaag acatatcccc aaaggcattt ctattctgag ccgtggaggg atattgttgt
      541 tgaatcctct cccacggcta aagccgggag attccacagt gttcatataa gctctctatg
      601 gctctcatct tggaagatta cagcatccat agcctcactt atactcgtta ctggatttgc
      661 gtgcgaaccg ctctgactct ggatattgaa tccaaagttg gcaggacata ccttgcctat
      721 tgcagtttgt ttaacccttg aaaccgcatc gctaaggaca gattccaatc cttgccagca
      781 cgatttttgt ctggctgtat caagggcact tattgcatat tttaagtagc caccagcatt
      841 ttcagctttg tgcttgctac ttgtattcgt atatattagt tctgagtaca tatttttaac
      901 ctctataata gtaatgatgt actggactaa ggggttcgct atccccacgg ctaaagccga
      961 agcttgcgct tactccttaa ccctacgatt tttgttaaga aaaggcagga aaaaactact
     1021 ttgccatctt caggacatcc tgctttgaga tatattcagg aaaatcccgt tgcatgacgg
     1081 gattttaaac ttaactccag aggaactaat ttttgagagt tatcactttt ttatttctac
     1141 aaccaaatta tgaccttcgg taattcctat attttcggtt gcatgcgacc cggcttccaa
     1201 ccacaatgtt tgtccggcat taaggtcaaa ttcaccggtt ttgccatcag gaaaggtcaa
     1261 tttgaacctg gtgttgttca tcacatagac aacatggctg tcaggatggt tatgcattgg
     1321 cgtcttttcg ccaggtttaa gccgaatatc taacaccctt actttttcat tttccatgag
     1381 cagcttgtaa gccttagctg ccactttcaa gacatctggt atttgttcca tattttttcc
     1441 tctttttgtt aattgctatt tttcgaccag tttaaccatc ctcatcacct ctcgcataaa
     1501 tgcgggcagg tccatcggcc aacgcgaggt gacgagatta ccatcaacga ccacatcttt
     1561 atcctcccaa attccgcctg cttcttgtat ctcttcgggc acaccatcat gccaataaga
     1621 agtaaggtgc ctccctttca caatgccggc cgacacaaga gtataggggc catgacagat
     1681 agaagcgacg ggtttatttt tggcaaagaa tgattttgct atctcctgcg cttttttgat
     1741 tttgcgcacg gttgcagctc cattcggagc tccgcccgga atgataagaa gatcgtattc
     1801 gccgggatca acttcttcaa tagttttatc gggttttaaa acatagcctt tttctccgct
     1861 tatctccttc aagctgggtg cagcgatgtc cacctgccat ccttcctcca gcaagcggaa
     1921 aacagggaag aagacttcca tatcctcaaa cttatcagca gtgagtacaa cagctttgcg
     1981 gttcgactta tttttaggaa gttgcataag tttctcctta attctatttt attgaatgct
     2041 tctccaatag aaatagtatt gtatattagg gcaaaaagat cacatccaaa tcaggaaata
     2101 catatgtagg ccaatgatga gcgccagtat ccatgcaaaa aagttttttt taaaaaatgg
     2161 aggtggatta accacctcat tggatcattt ttgacacccc gatagtgcga gctccgagga
     2221 tgcgcctttt ttcaatcgct gtttttaaat atttaaagta atctcatgga ctggaaagtg
     2281 actttagata ctcgaccaca tcaccctttt cttgatggga cagattcaat gttgataatt
     2341 tatcgtaatg atctactacg tccaatagtg tcttaaatcg tccatcatgg tagaagcggc
     2401 ctttgttctc cggtttcatg aatttgccgt tttcccgaat atatatcccg ccaagattca
     2461 tggtcttgta gagtccatca ggcgccctat ccgcctggaa actgtcaata ccgatttcag
     2521 atggcttatg cagattccat ccgggttcgg tccacagtgg ctcagaatgg cagttgttgc
     2581 acttggcctt attgctgaat agttcatccc cacgcgcttg caaaaacaaa tttactcact
     2641 ccactttcat aactgtgtct tgtctgtgca ttatcaaaga ctgctgtccc aaacaaatat
     2701 tcctttttca tatctgagaa ctgaacatca aactgacttc cggtcatcag ttttctgcca
     2761 tattccagtg tccaattgcc atccatgtag actgctttgc ccataatatc tgctctatcg
     2821 acagtcggag gccttacaat gatacctgca atctcatcgc ctgtattgta tgtgtcattg
     2881 aatggtttct tctgaacgtc aaatatccaa tagggcccag ctggctggtc tgccgaggtg
     2941 aaattcggtg cagttttgtt cgcatttatg ttatcatagt atggcacaag cccgggatcg
     3001 ctatgtctac ctgcatccgg tg
//
LOCUS       DATGBN010000075         6856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_5353, whole genome shotgun sequence.
ACCESSION   DATGBN010000075 DATGBN010000000
VERSION     DATGBN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5353"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VKL21_01610"
     CDS             <1..656
                     /locus_tag="VKL21_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HMB44496.1"
                     /translation="HSNIVALKAVIEELDEVGVKIILHAGDIVGYNPYPDETIDLFKK
                     KKIVSILGNHDRAFMTGDTSDFNPYAAAAMEWTRNTASRDSLNYILRLKDTVTIVANG
                     ERIVLFHESPGNFQEYIYPGNVTPDLFSNINGDVLVLGHTHIPFIMDYGTRGLVVNPG
                     SVGQPRDGNPDASFAVLNTVTRKIEHKRTKYDVEKVIRDIFAAQLPEALAYRLRAGR"
     gene            complement(1095..1535)
                     /locus_tag="VKL21_01615"
     CDS             complement(1095..1535)
                     /locus_tag="VKL21_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44497.1"
                     /translation="MIFIDTDIFVIEKLFKKDERYIVTNAFLNSDSKEKCTSIFNVFE
                     LVGIASFNLCTTDLKKLLKGFSEVYNIKILFPETSYESPDVFVEQLFANVFEKIILKM
                     SFSDALILSVAEEHSCSKFVTWNVKHFEGRTDIPVNTPEEMLTL"
     gene            complement(1532..1849)
                     /locus_tag="VKL21_01620"
     CDS             complement(1532..1849)
                     /locus_tag="VKL21_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HMB44498.1"
                     /translation="MTTNAVQIRMPSVLLKNIEDLVKQGFYKNKSEAIIDAVRHFVGF
                     GQARSEIATFIREELRGRKIKRDYSKKELDAIWEKVRLGQDWKERFGTDADNVMKELR
                     RRR"
     gene            1891..2985
                     /gene="dinB"
                     /locus_tag="VKL21_01625"
     CDS             1891..2985
                     /gene="dinB"
                     /locus_tag="VKL21_01625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMB44499.1"
                     /translation="MSDDARKPRIILHIDMDYFFAQCEEREHPEIKGKPVVICVYSGR
                     GGDSGAVSTSNYEARKLGVKAGISISHAKKLAPDAVFLPVNMELYHNKSGQVMEILKD
                     YSDKIEQESVDEAYCDLMEKVTDFEEARTLALKLKDEIRQLVGLTCSVGIGPNKLIAK
                     LASDYIKPDGLTVVKLEEVSNFLAPLKITELIGVGKKTGERLNELGVKFIADMSKLSI
                     DELVREFGQAKGLWLKQASQGIDDSPVEKRQGTGQIGRIITLKENTKELQIILDAIDR
                     LSGDIYMKLQSRNLGFKSISFIAISTDLKTHTKSLTMGTPAKDVETIRTKAHELVKSF
                     ISEQPMPLRRVGIRVSNLVEEKGQKTLGDY"
     gene            complement(3011..3601)
                     /locus_tag="VKL21_01630"
     CDS             complement(3011..3601)
                     /locus_tag="VKL21_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HMB44500.1"
                     /translation="MKKTYDKLLYELGRKNWWPADTPFEVVIGAVLTQQTKWENVERA
                     IRNLKERGLLEAEPLSKVDTEELEILIRCTGFFRQKAIRLKNISRFFHENPGLLEKPA
                     AQLREILLSLNGVGEETADSIVLYAADKPKFVIDAYTKRMCKCLGIEGDYRKLQSLFE
                     NSLPLDVPLYKEFHALIVEYGKRFCGKKRCDECILV"
     gene            complement(3598..4254)
                     /gene="npdG"
                     /locus_tag="VKL21_01635"
     CDS             complement(3598..4254)
                     /gene="npdG"
                     /locus_tag="VKL21_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HMB44501.1"
                     /translation="MKIAILGGTGSIGEGFALRWAGKHDILVCSREIDKAVNAADEYT
                     NILLYRGMECCGITGCGNEIAIQDADVVVLSVPYQGVISLLKYLNPHFKDQIVISLVV
                     PMTKNKWFEYTPPVQGSAALEIQEILPKSVKVVSSYHNVSARKLAKLDLSLDYDVVVC
                     GDDENAKQTVMELTREIKSLRPLDGGGLASSYMIESLTPFLINLAIRNKLSDLGVKFL
                     "
     gene            complement(4251..5174)
                     /gene="aglJ"
                     /locus_tag="VKL21_01640"
     CDS             complement(4251..5174)
                     /gene="aglJ"
                     /locus_tag="VKL21_01640"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HMB44502.1"
                     /translation="MNKEDVCILIPTLNEGKTIAGLVKEFKSLGYNDILVIDGHSTDD
                     TVSKAKNNGARVVVQTGKGKGQAINQAFRLLSSKYVVMVDGDGTYLPLEINKLLEPVI
                     SGQADHVIGNRFVNYQEGAFTGLNLFGNRILNKIFGLAYSVWLNDILSGYRAFNYIAY
                     KQIELKKTGFEVETEITVECVKKDLKIVEVPITYLTRVSGAATKLRPVRDGFRIASTI
                     YILTRTHNPLFYFNLIGLFLVMSGVIVGVYVINEWIQNVTHVPLTIFATLLILSGIMM
                     FIFAILSDLIVSMHRENMRTMRKILKEMDKS"
     gene            complement(5360..6781)
                     /locus_tag="VKL21_01645"
     CDS             complement(5360..6781)
                     /locus_tag="VKL21_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGF-pre-PGF domain-containing protein"
                     /protein_id="HMB44503.1"
                     /translation="MKQKIILSILFLLLTIQAASAVATLSVGAISYDSTVVKDESITV
                     SSSVTASTVTGTLTVNVRLTDNSGLFTIPTADQQLQFTTDGTKAISWTITATTSGTNA
                     APFTITASGDDGSSAPANPSSSAVTVKDRPLITISTSSNVSSIDSGGVASVSYIVSNS
                     ASDGAADATNVNITLTSPVGWNLVSGTNPYSLGTIAPETSQSGSWIVKADSPSSSNTF
                     TLDVVSTIPGGTVSKTVSISGPSGGGDSNTGGSGGGGGGGGGGKSDENYTNIEVVEKY
                     DMEISKNALTSYRFKDPKNPIMFVNITGNISIGLITATIELLKGTSTIVKVPPDGLVY
                     KNANIWVGTSGMATPKNIKEALIKFKVDNSWMRANGVLASDVVLVKWDGSSWIKLETK
                     VSSKDDTNTYLEGKTNSFSPFAIIVVKSTGTSSQVTAPGGTSTPQKVEETTRSKKKGT
                     PGFDIVLVAASISALYLLGRKRR"
BASE COUNT         1944 a         1455 c         1400 g         2057 t
ORIGIN      
        1 tccattccaa tatagttgcc ctgaaagctg tgattgagga actggatgag gtgggggtga
       61 agataatact gcatgcaggt gacattgttg gatacaaccc ttacccggat gaaactatcg
      121 atctatttaa gaagaaaaag atcgtgtcga ttctggggaa ccacgacagg gcgttcatga
      181 caggtgatac ttctgatttt aatccgtatg ctgcggcagc gatggaatgg acaagaaata
      241 ccgcgtcccg ggatagtttg aattatattt tgaggcttaa ggatacagtg actattgtgg
      301 caaatggtga aaggatagtc ctattccacg aaagccctgg taattttcag gaatatattt
      361 atccaggaaa tgtaacgcct gacctttttt ccaatatcaa tggcgatgtc cttgttctgg
      421 ggcataccca tataccattc ataatggatt atggaacgcg tggtctggta gtgaatccag
      481 gttcagtcgg gcaaccaagg gatggaaatc ctgatgcttc gttcgctgtt cttaacacag
      541 ttacacggaa gatcgaacat aaaagaacaa aatatgatgt cgaaaaagtg atcagagaca
      601 tttttgcagc acaacttccg gaggcactgg cttacaggtt gagagccggg cgttagtaaa
      661 tgaatcattt agtgttaatg tcctgcgaca tgccggggaa aaatcatatc tttccataac
      721 gtcatgcctt aaaatacgat taagggatat ttcatttcaa ttcgccaaga ctttcagaat
      781 agcgcgcatc cagattcatt attaaaagat gctaagagcc tggatttcaa agtatttaca
      841 ggttaaattt gtctattaat gtggataaac ctgcaactct aatatcgaga aaaattgata
      901 tgataataaa ctatacatca ttctatgaaa tgctgtccga cagataaaaa tattaagaaa
      961 tattggatgg aaaaacttga aagcgaatca aacaatttgt caaaatacaa atattacagg
     1021 atagaaagtg tagcattgag atttttagtc cattgtggac aggggcagag aaatctcagc
     1081 ccttaatcct gcaactataa ggttagcatc tcttccggag tattcacggg aatatctgtt
     1141 cgaccttcaa agtgttttac gttccatgtc acaaatttag agcaagaatg ttcttctgca
     1201 acgctcaata tcagagcatc agaaaaactc atcttcagga ttattttttc aaatacatta
     1261 gcaaataact gttctacgaa gacatcggga gattcgtatg aagtttcagg aaacagtatc
     1321 tttatattat aaacttcaga aaatcctttt agcaatttct tcaaatcggt tgtgcagaga
     1381 ttaaaagatg caatgccaac aagttcaaac acgttgaaaa ttgaagtgca tttttctttt
     1441 gaatcgctgt ttagaaacgc atttgtgacg atatatcttt catccttctt gaatagcttt
     1501 tcaatcacaa aaatatccgt gtcgatgaat atcatcgtct tctccggagt tctttcatga
     1561 cgttatcggc atccgttccg aagcgttctt tccagtcctg tccgagtctt actttttccc
     1621 atattgcatc caattctttt tttgaataat cacgctttat ttttcttcca cgcaactctt
     1681 ctcttatgaa tgtggctatt tcgcttcttg cctgcccaaa acccacaaaa tgacgtacag
     1741 catcgataat tgcttcgctt ttgtttttat aaaaaccctg cttaaccaga tcttctatgt
     1801 tttttagtaa tacagatggc attcttattt gtactgcatt agttgtcata cattatttaa
     1861 atatacattg atatacataa atgctatcct atgtcagatg atgcaagaaa acccaggatc
     1921 atactccata ttgatatgga ctatttcttc gcccagtgcg aagagagaga gcatccggaa
     1981 ataaaaggaa agcctgttgt tatctgcgtc tattcggggc gcggtgggga ttcaggagcg
     2041 gtaagtacca gcaattatga ggctcgaaaa ttaggagtaa aagccggaat ttccataagc
     2101 catgcaaaaa aactggctcc agatgcggta ttcttaccag tgaatatgga actttaccat
     2161 aacaagtccg gtcaggtgat ggagatatta aaagattatt ctgacaaaat cgagcaggag
     2221 agtgtagatg aagcatattg cgatctgatg gaaaaagtca ctgattttga agaggcaaga
     2281 acgctggccc tgaaattaaa agatgagata agacaacttg tggggctgac gtgttctgtc
     2341 ggcatcgggc caaataaact tattgccaag ttagcatcag attatataaa acctgatggc
     2401 ctgacagttg tcaagttgga agaagtttca aattttttgg ccccccttaa aatcactgaa
     2461 cttataggag tgggtaaaaa aacaggagaa agactcaatg aacttggggt aaagttcatc
     2521 gcagatatgt cgaagctctc gatagacgaa cttgttcgag aattcggaca ggcgaagggg
     2581 ttatggttaa aacaggcttc gcagggtatc gatgattcgc ctgttgagaa acgccaggga
     2641 acggggcaga tcggcaggat aataaccctg aaagaaaata caaaggagtt acaaattatc
     2701 cttgacgcta tcgatcggct ttcgggggat atttacatga aactgcaaag caggaatctc
     2761 ggttttaaat cgatctcatt tatcgctatt tccactgacc ttaaaaccca cactaaaagt
     2821 cttacgatgg gaactcctgc aaaagatgtt gagactatta gaaccaaagc ccatgaactc
     2881 gtgaaatcat tcatttcaga acaaccaatg ccccttagaa gagtgggaat cagggtttcg
     2941 aatctggtgg aagaaaaagg acaaaagacg ctgggagatt attgagatcg ggcctttccg
     3001 aaagggtagt ttacacgagt atacactcat cgcagcgttt ttttccgcag aatcgctttc
     3061 catattctac gatcagggca tggaattcct tatacagtgg gacatcaagc ggcagcgaat
     3121 tctcaaagag ggattgcagt ttcctgtaat cgccttctat ccccaggcat ttgcacatcc
     3181 tttttgtata ggcatctatc acgaattttg gcttatctgc cgcatataat acgatgctat
     3241 cagcagtttc ttcacctacg ccgttgaggg aaagtagtat ttcacgaagc tgggcggcag
     3301 gtttttcaag aagacctgga ttttcatgga aaaatcttga aatgtttttt aaccttattg
     3361 ccttctgcct gaaaaaccct gtgcatctga taaggatttc caattcttca gtatctactt
     3421 tggaaagtgg ttctgcttcc aacaatcccc tttctttcaa attccgaatt gcacgttcta
     3481 cattctccca ttttgtttgc tgtgtgagga cagccccgat aacaacctca aaaggtgtat
     3541 ctgccggcca ccagttctta cgaccaagtt cataaaggag cttatcatag gtctttttca
     3601 taaaaatttt acgccaaggt cagaaagctt gttgcggata gcaagattga tcaggaatgg
     3661 tgtaagggat tcgatcatat aagaagaggc aagacccccc ccatcaagag gccgcaggga
     3721 ttttatttcc cgcgtaagct ccataacggt ttgctttgca ttctcatcat cgccgcaaac
     3781 cacaacatca taatcaagac tcaaatcaag tttggctaat tttcgcgccg aaacattatg
     3841 gtatgacgac acaactttca cggattttgg aagaatctcc tgtatctcaa gcgctgcgct
     3901 tccctgtact ggcggagtgt attcaaacca tttattctta gtcatgggga ctacgagcga
     3961 aatcacgatc tggtccttaa aatgaggatt gaggtatttc aaaagggaaa ttacaccctg
     4021 gtatgggacg gaaagcacaa ccacatccgc gtcctgaata gcgatttcat tcccgcaccc
     4081 cgtaatgccg caacactcca tacccctgta taaaagtata tttgtgtact catccgccgc
     4141 attgaccgct ttgtcaattt cacgggaaca aaccaatata tcgtgttttc ccgcccatcg
     4201 cagtgcgaat ccttccccga tgcttcctgt tcctccaagt atagcaatct tcatgattta
     4261 tccatttcct ttaagatttt tcgcattgta cgcatatttt ccctgtgcat tgaaactata
     4321 aggtcgctga ggatcgcaaa aatgaacatc attattccag atagaataag aagagtcgca
     4381 aagattgtca agggaacatg cgttacattt tgtatccatt catttatgac ataaacacca
     4441 acaattaccc cggacataac taaaaacaac cctatcaaat tgaaataaaa tagaggatta
     4501 tgcgtccttg taagaatata tattgtagaa gcgatccgaa agccatccct gacaggccga
     4561 agtttagtgg ctgcaccgct tactcttgta agatatgtta tgggtacctc cacgattttc
     4621 aaatcctttt tgacgcattc aactgtaatt tcggtctcga cttcaaaacc ggtctttttc
     4681 aattcgattt gtttgtaagc gatataattg aatgccctgt atccggaaag tatgtcattt
     4741 aaccagacgc tgtaagctaa accgaatatc ttgttcaata tccggttccc gaataagttc
     4801 aaacctgtaa atgccccttc ctggtaattt acgaaccggt ttcctataac gtggtcagct
     4861 tgccctgata tgaccggttc aagtaatttg tttatttcca ggggaagata tgttccatcc
     4921 ccatcgacca ttactacata tttgctcgac aataaacgaa acgcctgatt tatagcctgt
     4981 ccttttcctt tgccggtttg caccacgact cttgcgccat tgtttttcgc ttttgatacg
     5041 gtatcatccg tgctatgacc gtcaataaca agaatatcgt tataacccag tgatttgaat
     5101 tcttttacca atccggctat tgtttttccc tcattgaggg ttggtattaa gatacagaca
     5161 tcctctttat tcatttaaag tccttatatt tttattaata cttaattgtt ggtcaatctc
     5221 tggaaacacc ggagtcattg gttaaggaat ttaatgctgc agggtcattg aaaatacttc
     5281 ttagtacatt ttgttgccgc caggagacat attggtgata tttatttcga tgtcattctt
     5341 cttttgagaa gatgatttct catcttctct ttcgccctaa caggtataat gcagaaattg
     5401 aagcagcaac caggactata tcaaatccag gtgtgccctt cttctttgat cttgttgttt
     5461 cttcgacctt ttgaggtgtc gatgtcccac ccggggcggt cacctgggag gatgttccgg
     5521 tagattttac cactattatt gcaaatggtg agaaagaatt ggtctttccc tctaagtatg
     5581 tgttggtgtc gtcttttgac gatacctttg tctcaagctt tatccagctg ctgccgtccc
     5641 acttcaccag cacgacatca cttgccaaaa caccgtttgc cctcatccat gagttatcta
     5701 ctttgaattt gatgagagct tccttgatgt tctttggcgt tgccatgccc gaggttccaa
     5761 cccagatgtt ggcgttctta taaacaagtc catcaggtgg taccttcacg attgtcgaag
     5821 taccctttaa taattctatg gttgccgtga ttagaccaat gctgatattg cctgtgatat
     5881 tgacgaacat tatgggattc ttaggatcct tgaacctgta ggatgttaga gcattctttg
     5941 aaatctccat atcatatttc tcgaccactt cgatatttgt gtagttctca tcagacttgc
     6001 cgcctccgcc ccctcctcca ccgccaccac tgccaccggt attactgtct cctccgcctg
     6061 atgggccact gatagataca gttttggata cagttccacc cggaatggtg gatacaacat
     6121 ctaaagtaaa tgtgttagat gaagacggag aatccgcctt tacgatccat gaacctgatt
     6181 gggaagtctc cggggcgatc gttccaagcg agtatggatt cgtgccgctc acaagattcc
     6241 acccaacggg acttgttaat gttatattga catttgtagc atctgcagcg ccatctgaag
     6301 cgctgtttga aacaatatag ctaacagaag caacacctcc ggagtctatc gaagatacat
     6361 tagaggatgt tgatattgtt ataagaggcc tgtccttaac ggtcaccgcc gaagatgatg
     6421 gatttgccgg ggcactgctg ccgtcatcac ccgatgccgt gatcgtgaag ggcgctgcat
     6481 ttgttcccga tgttgttgct gttatcgtcc atgaaattgc ctttgtacca tcggtggtga
     6541 actgcaattg ctggtcagct gtggggatgg tgaacaatcc cgagttatct gtcagcctga
     6601 catttaccgt aagcgtgcct gtcaccgtag atgcagtcac agatgatgaa actgttatac
     6661 tctcatcctt cactacagta ctgtcatagc tgatcgctcc aacagataat gttgcaaccg
     6721 ctgatgctgc ctggatcgtc aggagcaaaa acagaattga taaaattatt ttttgtttca
     6781 tttcttgtct ccgcctcctg gcgcctctcc tttaccacta tttgttttgt ccattatgaa
     6841 gaaatttatc ttcaat
//
LOCUS       DATGBN010000076         5759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_510466, whole genome shotgun sequence.
ACCESSION   DATGBN010000076 DATGBN010000000
VERSION     DATGBN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510466"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VKL21_01650"
     CDS             <1..761
                     /locus_tag="VKL21_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HMB44504.1"
                     /translation="RVRKDLEIIKPDENLSHAANFLYMMGGEVPDAGTARDFDTCLIL
                     HAEHSFNASTFTARVVASTHAHMYACIGAALGALSGDLHGGANMAVMKMLIEIDKIEK
                     VEKWIKTKIDAGEKIMGMGHAVYKTMDPRARILYNISEKLGKRTKNSKWFDLSRKIES
                     ISRDEFRKRKGKEIYPNVDFYSPSVYSQMGIDPDLFTSIFAVSRISGWCAHIIEEKFA
                     QAQPEPAIYRPEADYVGNYCGIEGCKYVPVSERK"
     gene            956..1147
                     /locus_tag="VKL21_01655"
     CDS             956..1147
                     /locus_tag="VKL21_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HMB44505.1"
                     /translation="MQIDPICGMQVDENTAQYKSEYKGKTYYFCASGCKKAFDSDPEK
                     YQKGGPVGMGEKKPWWKFW"
     gene            1152..1592
                     /locus_tag="VKL21_01660"
     CDS             1152..1592
                     /locus_tag="VKL21_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44506.1"
                     /translation="MKLSGIIIALLILIAAAVSGCLSGKEPQTENPNSKVPTSTDIIS
                     TADSSEKRVNSEAGVTITAVNLGNNAFDIKLDTHSGSLDYEMAGISYIRDSKGNMIKP
                     ESWDGGFGGHHFEGTLKFPEFDDSAGFELVIQDVAGVKERVLKW"
     gene            1586..1798
                     /locus_tag="VKL21_01665"
     CDS             1586..1798
                     /locus_tag="VKL21_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44507.1"
                     /translation="MVNKMSLKQGIKNLLKNPFVFGASAGMLVVLFNISIASLAEGSL
                     EQGYQVSGFLNGSQEFAGDGFWNLEI"
     gene            1886..3010
                     /locus_tag="VKL21_01670"
     CDS             1886..3010
                     /locus_tag="VKL21_01670"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HMB44508.1"
                     /translation="MNKFKVNKTIVVGLVIWLSLSIVLGSAGTLTSEKVAAAPVIDGT
                     PEALWNKATAMTVNVAGGANTGAHVVTLKSVYTDDSVYFLAAWNDPTESLRREPWVKQ
                     ANNTWKQLKDPDDKGGDDNKYYEDKFAQIWNINSPGFETTGCFATCHAGEAGKAYGNK
                     YTANPGEMADIWHAKLVRTNPSGYIDDQYVDSTRYDKDKAPDAGRKSDPGLVPYYTNI
                     NAANNSPNYTSADQPAPPYWIIDAEKQPFKDTYKANDEIAVIITRPPDGDRADIKARA
                     VYDDGKWTMEYGRKLTTGSQYDVQFSDMKKEYSFGTAIFDGAQVRHSFETGVSKLVFA
                     AQAAPTPTSPPAPTATKKAPAFEALLAISALLAAVLVKRR"
     gene            complement(3039..3767)
                     /locus_tag="VKL21_01675"
     CDS             complement(3039..3767)
                     /locus_tag="VKL21_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HMB44509.1"
                     /translation="MQPSELSIFIVFLAGVVTIMKPCCLPLVPVIFSGSGGHRLRPLA
                     IVSGLTLSFTTMGVLVSAFGATFGAYTDYLRNIAILFIISMGLVLFDEDVNMEFMKIS
                     GSITQRLQGIGLFNRFSSRMPPGSLMGGFFLGMSLGVLWIPCVGPILGAVLALVASVG
                     NMTYGASMLFVYSIGMSLPMLSIAYYGKKITNRYKWFSRNGELLKKLSGLVLILIGIM
                     LLFGVDKLIIKWLSPYFPTTFYGI"
     gene            3911..5542
                     /locus_tag="VKL21_01680"
     CDS             3911..5542
                     /locus_tag="VKL21_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB44510.1"
                     /translation="MNEKILILSVILLLFAFAFPVNANEVGNMDMSDPSSMNMDQPAA
                     GHGMNGEVNWYAIALFIAIIGATGYYASKTEKLRKINFLNYAPLKSLLKSRWYPLIFV
                     LPTMIIFTIIVVKLFFGSVEASSNFGSIMMWIFLWPLLPVLFLLFGRLWCSVCPLSRV
                     SDEVQKKVGMHRKVPKFLQTYGVWIIIFAFLAITWSDVIFGVVESPLNTGILLLFILI
                     GVVFMGAVFEKRAWCRYLCFLGGLSSNYSMSSALELRTDSEVCKTCKTPLCYKGDGKT
                     EGCSMFEYPRTMDSNRACNFCSNCIKTCDHDAIRITPRPPTSELWFIKKPRFEESFLA
                     TALIGIVISQTMIMLEVWEPFMTWFENTTGITNFPVAWTLIFAGAMLLPVLLMLAASF
                     ISSIIPGKTSNSDLISDQTAELSVVSKDSPETTLSGFIRYGYALIPLGLGIHLAHNAK
                     HFLGEGFAVFYSSASLVGWNITGDLSILNMPTIQVIQYILSILGILGAIYTAYKISTN
                     NPNSKSSVLPYIVLILMFGIIALWMYSIPMAARAH"
BASE COUNT         1761 a         1140 c         1314 g         1544 t
ORIGIN      
        1 atagggtgcg aaaggatctt gaaataataa agccggatga gaatctcagc catgcggcta
       61 acttccttta tatgatgggt ggagaagtcc cagatgctgg gaccgccagg gattttgaca
      121 cctgcctcat acttcatgcc gagcattctt ttaatgcctc cacgtttaca gcaagggtag
      181 tggcatcaac acacgcacat atgtatgcct gtatcggcgc agcgctgggg gcgctttcag
      241 gcgacctgca cgggggtgcc aatatggcgg ttatgaaaat gcttattgaa atagataaga
      301 ttgaaaaagt agagaaatgg ataaaaacaa aaattgatgc cggggaaaag ataatgggta
      361 tgggtcatgc agtttacaaa accatggacc cccgggccag gatactttat aatatttcag
      421 aaaaactggg aaaaagaaca aaaaattcaa aatggtttga tttatcccgg aaaattgaaa
      481 gtatttcaag ggatgagttt agaaaacgaa agggtaaaga gatttatccc aatgtggatt
      541 tctacagtcc gtctgtttat tctcaaatgg gaatcgatcc tgacctgttt acatcaattt
      601 ttgccgtatc aagaatttca ggatggtgcg ctcatattat tgaggaaaaa tttgcccagg
      661 cccagccaga acctgccatt tataggccgg aagctgatta tgttggaaat tattgcggga
      721 tcgaaggatg caaatatgtg cctgtatctg agcgaaaatg atcattttaa aatcggctta
      781 atttatttta ttaaggaaaa aatcttacga agaagaacaa aagaatacac ccttgaagaa
      841 cttgagattg aaaaaggggt gttagaacaa atacatcgct tccgctataa gatatctact
      901 ttacaccaaa cgtaaagtac aaatattagt aagaacaata aaaggagata tgaatatgca
      961 aatagaccca atttgtggaa tgcaggtaga tgaaaataca gcacagtaca aatcagaata
     1021 taaagggaag acctattatt tttgtgcgtc cggatgtaag aaagcatttg attctgatcc
     1081 cgaaaaatac cagaagggcg gccctgtggg catgggtgag aaaaaaccat ggtggaagtt
     1141 ctggtaatta catgaaacta tctggaatta taatcgcgct tttgatcttg attgcggcag
     1201 cagtcagcgg gtgtttatcc ggcaaagaac cccagactga aaatcccaat tccaaagttc
     1261 ccacttctac agatataata tctacggcgg actcatctga aaagagagta aactcagaag
     1321 caggagtaac tatcactgcg gtgaatcttg gcaataatgc ctttgatatc aaactcgata
     1381 cacattcagg atcgctggat tatgagatgg ccgggatatc ctatatccgc gatagcaagg
     1441 gcaatatgat caaaccggag tcatgggacg gaggcttcgg aggccatcat tttgaaggca
     1501 cattgaaatt cccggaattt gatgatagcg ctggttttga actcgttatc caggacgttg
     1561 caggagtgaa agaaagagtc ctaaaatggt aaataaaatg tctttaaaac agggcatcaa
     1621 aaacctgtta aaaaatccct ttgttttcgg ggcaagcgca ggaatgcttg tggttctttt
     1681 caatatctcg attgcctccc ttgccgaagg atcacttgag cagggatacc aggtatctgg
     1741 gtttctcaat gggagccagg aatttgctgg cgatggcttt tggaatttag agatttgaaa
     1801 atccttataa gctatacctg acaatagtaa ttattgcatt atataaaaag gggaaatata
     1861 gtgcatgtaa tacacgggag aaattatgaa taaatttaaa gtcaataaaa caatagtggt
     1921 aggattggtg atatggctgt cgctaagcat tgtattgggg tcagctggca cccttacttc
     1981 tgaaaaggtg gcagctgcac cggtcataga tggcacgcct gaagcattat ggaacaaggc
     2041 aactgccatg acggtgaatg tagctggcgg agcaaataca ggcgcacatg tagtgacttt
     2101 gaaaagtgtt tacacggatg attctgtgta tttccttgcg gcatggaacg atcccacaga
     2161 aagcttaagg cgcgagccct gggtcaaaca ggcgaacaat acatggaagc agctcaagga
     2221 tccggatgat aaaggcggag acgacaataa gtactatgag gacaagttcg ctcaaatctg
     2281 gaatatcaac tccccgggat tcgagaccac cgggtgtttc gcaacatgcc atgctggaga
     2341 ggctggaaaa gcatatggta ataaatatac tgcaaacccg ggtgaaatgg cggatatctg
     2401 gcatgcaaaa cttgtacgaa caaatccatc aggttatata gatgaccaat atgtggactc
     2461 cacacgttac gataaggaca aagcgccaga cgcgggaagg aagagtgatc ccggactggt
     2521 gccttattat acaaatatca atgctgccaa taactctccg aactatacat cggcagacca
     2581 gcccgctcca ccctattgga tcattgatgc cgagaagcag cccttcaagg atacatacaa
     2641 ggctaacgac gaaatagcag ttatcataac gagacctcct gatggggaca gggctgatat
     2701 taaagccagg gctgtttatg atgatggcaa gtggacgatg gaatatggca ggaagctgac
     2761 aacagggagc cagtatgatg tgcagttctc agacatgaaa aaggagtatt cctttggaac
     2821 tgcgatcttt gatggtgccc aggtaaggca tagtttcgag accggcgtga gcaagctggt
     2881 atttgctgcg caagccgccc caacaccaac ctcacctcca gccccgacag caacgaaaaa
     2941 agcgccagca tttgaagccc tcctggcaat ttcagcgctg ctcgcggcag tactggttaa
     3001 aagaaggtaa aacaatacct tctttttttt ttatcttctt aaattccata aaaagttgtc
     3061 gggaaatatg gggatagcca tttgataatg agcttatcga cgccaaagag caacattatt
     3121 ccaataagta tcagcacaag accggacaat ttcttaagaa gttccccgtt ccttgaaaac
     3181 catttgtacc tgttcgttat tttctttcca taataggcaa tagaaagcat gggaaggctc
     3241 atccctatgg aatatacgaa tagcatcgat gcaccgtatg tcatatttcc cactgatgcg
     3301 accagggcaa gaactgctcc aaggatcggg ccaacgcacg ggatccagag aacgccaagc
     3361 gacattccca ggaaaaatcc tcccatcagg cttccaggcg gcattctgga agagaaccta
     3421 ttgaataatc cgattccctg caaacgttgt gttattgaac ccgatatctt catgaactcc
     3481 atatttacat cttcatcaaa aagtacaaga cccatactta ttatgaacag gatcgcaatg
     3541 ttgcgtaaat aatcagtata tgctccaaat gtagcgccaa aagcagaaac cagcactccc
     3601 atggtcgtaa agcttaaggt caagccggat acgatagcaa gaggtcgaag cctgtgtcct
     3661 cctgaacctg aaaatataac tggcacaagt gggaggcaac agggtttcat gatcgtaaca
     3721 actcctgcta agaagacgat aaatattgaa agttctgatg gctgcatttg tttttaacct
     3781 tccttcctat tgatttattt aatggatatg aaactaccct ctactttaca tatgatgtaa
     3841 acaaagtata aatacccgat gttacctaat agatttactt tgcattagat gtaaagaagg
     3901 agaaaatgga atgaacgaaa aaatattgat attaagtgtg attttattgt tgtttgcatt
     3961 tgcttttcct gtgaatgcga atgaagtagg taatatggat atgagcgacc ccagctcaat
     4021 gaacatggat caacctgctg caggacatgg catgaatggg gaagtgaact ggtatgcgat
     4081 agcactattt atagcaataa tcggagctac aggatattat gcttcaaaga cggaaaaact
     4141 caggaagatc aatttcctga attatgcccc gttaaaatct cttcttaaaa gccgctggta
     4201 tcccctgatt ttcgtcctgc ctacgatgat aatattcaca ataatcgtgg taaaactttt
     4261 ctttggcagt gttgaagcat cttctaattt cggctcaata atgatgtgga tattcctatg
     4321 gcctctttta cccgtccttt tcctgctgtt cggaaggctg tggtgttcgg tctgtcctct
     4381 ttcaagagtg agcgatgaag tgcagaaaaa agtgggcatg catagaaaag tgccaaaatt
     4441 ccttcagacg tatggtgtct ggatcattat tttcgcattt cttgccataa catggtctga
     4501 tgttatcttt ggcgttgttg aatctccttt aaatacagga attctattat tgttcatatt
     4561 aattggcgta gttttcatgg gagcagtatt tgaaaagaga gcctggtgcc gttatttgtg
     4621 tttccttggc ggtctttcaa gcaactattc catgagttca gcacttgaac tcaggacaga
     4681 cagcgaagtc tgtaagacct gcaagacgcc tttatgttat aagggtgatg gaaaaacaga
     4741 aggctgttcg atgtttgaat atccaaggac catggattcc aaccgtgcct gcaacttctg
     4801 ctctaattgc ataaaaacat gcgatcatga tgcaataaga ataaccccgc gcccgcccac
     4861 aagcgaactc tggttcataa agaaaccgcg gtttgaagaa tcattccttg ccacggcttt
     4921 aattgggatc gttataagcc agaccatgat aatgcttgag gtatgggaac cattcatgac
     4981 atggtttgag aataccactg gcatcacgaa tttcccggtg gcatggactt tgatctttgc
     5041 cggggcaatg ctcttaccag tcctgctgat gcttgctgca agttttattt caagcataat
     5101 tccgggaaaa acatctaatt ccgatctgat atcagatcag acagcagaat tgagtgttgt
     5161 atcgaaggat tccccggaaa caactctttc cggctttatt cgttatggat atgctcttat
     5221 tcccctggga ctgggaatac accttgcgca taatgccaag catttccttg gtgaaggttt
     5281 tgccgtgttc tatagttcgg catctcttgt ggggtggaat attacggggg acctatcaat
     5341 attgaacatg cccacgattc aggtcatcca gtatattctc tcgatactcg ggattctggg
     5401 cgccatatat acggcatata agatatccac caataacccc aattcaaagt catccgtgct
     5461 gccttatatc gttttgatat tgatgtttgg tattatagca ctgtggatgt actctatacc
     5521 catggctgcg agggcgcact aagaaaacat cagagaataa gaaacacagg gatttaatag
     5581 accttttgtg tttttatttt tttaacaggc gcgctggaag atgccttata tttaacttcc
     5641 gaacttattg gaacctctaa attgttcaat ataggtggta gagaactcgc agagatgtga
     5701 gtccggaaag ggggtattaa tttttgtatg aattcacaga tttcctgtat gcacccgag
//
LOCUS       DATGBN010000077         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_200022, whole genome shotgun sequence.
ACCESSION   DATGBN010000077 DATGBN010000000
VERSION     DATGBN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200022"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1617
                     /locus_tag="VKL21_01685"
     CDS             178..1617
                     /locus_tag="VKL21_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMB44511.1"
                     /translation="MLAAIPPLGQLIQIPAIYIVEKYRVRREICIVSTAVSRSFWLLI
                     ALIPFLVLIINPMTLLIIALIINAAFSAIGGCSWNSWMRDLVPVDRMGSFFGKRMIYA
                     SIVGIFFSLAASIFIDYWKQLYPEKELYAYSYIFFAGFLTGMLGLYFLSQTPEPRIVI
                     MQEKINFSRLLMQPFKDTNFKNLIIFLGSWNFAVNLAAPFFTVYMLMRLKLDMSTIIL
                     LMLLNQVVSIAFLHLWGKYSDRFSNKSILSINGPLFIGCILAWTFTYALTMPLLVVIH
                     ILMGISTAGITLSSGNIGLKLAPRGQATSYLAASSFVNSLAAGIAPVLGGLFADFFAD
                     EKIYVKIFWESPGIIRDIQPLYFQSWDFFFLLAFLIGFYSIHRLALVKEVGEVEEKIV
                     INELMSEVRRDMRSFSTASGLRSTIQFPFSVLRSTIGNVNVLNDFKCSLRNLNSRRVI
                     KSTMFRSAFKKRLKSISRREISEEINKPL"
     gene            complement(1601..2806)
                     /locus_tag="VKL21_01690"
     CDS             complement(1601..2806)
                     /locus_tag="VKL21_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB44512.1"
                     /translation="MKIAIVAPSVWYRIAPPHESKYGGAEKIVYWICEKLVEWGHDVT
                     LFATGDSITSAKLVPTYPCSVLGQDIKNGQDSKISISWNDITDNFLGLRKALEVEGEF
                     EVIHSHVYGDPLVTSSLNGSGVQVLSTIHNSMSAKDMGWENIMLYRSAKNRKHHSFVA
                     ISKSQTIHDDAGLSYKDVVYNGIDLSSYKTRRRIKRKNDTLLCLSRIDPQKGIECAIN
                     AAQEAGMKLIIAGRKHPGKEEEFFKDRIEPRLQKGVLEYAGEVSDEDKIKLLSATTGV
                     IFPSLWREPFGLVPIEAGACSTPLIGFECGAIPEIVMNGKTGFLVKPRNKCGKPNIKG
                     LVKAIKKIDQIDPKDCFDHVKEKFTADIMVENYLNIYKELSNKTTDALKPSIWDQSNK
                     HGNFPIEVY"
     gene            2943..4037
                     /locus_tag="VKL21_01695"
     CDS             2943..4037
                     /locus_tag="VKL21_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB44513.1"
                     /translation="MRIAMFCSSLLPTPPANKEKRIHAPLHLTALLASEMAKRGHSVT
                     LFGAKGTKSKANVVDLDLPPLFNHPQIGDSIDLKNHSAINFYEQVFISEIYRRAAEGE
                     FDIIHIHPVDLSINFAVNCRVPTVFTLHDPISPWRRFIYNTHKMNKNIFYVSISDSQR
                     LPLKDINFIDTIYNGIDLCRYPFNALPGSYLLSASRLVPEKGVDVAIRAAKRTGLPLK
                     ITGEPASDNNSYWMEKIKPDVGGKISYEGMVSTRRMFSIYKNAAALLFPIKWEEPFGL
                     TMIEAMACGTPVIAFNRGSVKEIVVDGRTGFIVDDLNGMIRSIKKINSIDRMECRRHV
                     EENFSMKKMVDKYEKVYEKILFKSQIPRSR"
BASE COUNT         1186 a          878 c          838 g         1394 t
ORIGIN      
        1 ctgccgtact tacgatacaa atctctcttc tgaccctgta tttctccaca atatatattg
       61 caggtatctg gatcaactgt ccaagaggcg ggatggctgc aagaataccg ataatcaggt
      121 tagaagctcc aagttttagc gcaaaagcca caaggaatat tccacctgat tatcggtatt
      181 cttgcagcca tcccgcctct tggacagctg atccagatac ctgcaatata tattgtggag
      241 aaatacaggg tcagaagaga gatttgtatc gtaagtacgg cagtgagcag gtcattctgg
      301 cttttgattg ctctgattcc tttcctggtt cttattataa atcccatgac cctgctcatc
      361 attgctttaa taataaatgc ggctttctca gccatcggag gctgcagctg gaactcatgg
      421 atgcgcgacc ttgttcctgt ggatcgaatg ggatcttttt tcggtaaacg aatgatctat
      481 gcaagtatag tgggaatttt cttcagtctt gctgctagta tttttataga ttactggaaa
      541 caattatatc ctgaaaaaga gttatatgca tattcatata tttttttcgc tggatttctg
      601 acaggtatgc tgggactgta ttttctttca cagacacctg agcccagaat tgttatcatg
      661 caagaaaaga tcaatttctc aaggctgctt atgcagcctt tcaaggatac caatttcaag
      721 aacttgatca ttttccttgg atcctggaat tttgccgtaa acctggcagc tccttttttc
      781 acggtttata tgcttatgag attaaagctt gatatgtcaa ctattatact tctcatgcta
      841 ttaaaccagg ttgttagtat tgcattcttg catttgtggg gaaaatattc tgacaggttc
      901 agcaataaat cgatactcag cataaatggt cctttattca taggatgcat tcttgcatgg
      961 actttcacgt atgcattgac gatgccgtta ttggtggtta tacatatcct gatgggtatt
     1021 tccacagcag gaataactct ttcatcagga aatatcgggc tcaaattagc gccaagagga
     1081 caggccacgt catatttagc agcaagcagt tttgttaatt cgttagcagc aggaattgcc
     1141 ccggtgcttg gtggattgtt tgctgatttt tttgccgacg aaaaaattta tgtgaaaatt
     1201 ttctgggaaa gtccggggat aattcgtgat attcaacccc tgtattttca atcctgggat
     1261 ttcttttttc ttttagcatt cctgatcggt ttttattcca tacatagatt ggcattggta
     1321 aaagaagttg gagaagttga ggagaagatc gtaataaatg aacttatgtc tgaagtaaga
     1381 agggatatga ggtcattttc cactgcaagc gggttgagaa gcacgatcca gtttccattt
     1441 tctgttttga ggagcaccat tgggaatgta aatgttctga acgattttaa atgttctctc
     1501 agaaatttaa atagtagacg ggtgattaag agtactatgt tcagatctgc atttaaaaag
     1561 cgcttaaaga gcatctcacg aagggagata tccgaggaga tcaataaacc tctataggaa
     1621 aatttccatg cttatttgat tgatcccaga ttgaaggctt taatgcatcc gttgttttat
     1681 tgctcaattc cttatatata ttcaaataat tttcaaccat tatatctgcc gtgaattttt
     1741 ctttaacatg atcaaaacag tcttttgggt ctatttgatc tatttttttt attgctttca
     1801 ctaatccttt aatgtttggc tttccgcatt tattacgcgg cttaaccaaa aaaccagttt
     1861 tgccattcat aactatttca ggaattgccc cgcattcaaa accgattaaa ggcgtactgc
     1921 aagcgccagc ttcaataggt accagaccaa agggttctct ccataaagat ggaaaaatta
     1981 cccctgttgt tgcactgagg agcttgattt tatcttcatc gcttacctca ccagcgtatt
     2041 caagcactcc tttttgtaag cgcggttcta tgcggtcctt aaagaattct tcttccttac
     2101 ctggatgttt tcttccggct ataataagtt tcattcccgc ttcttgcgct gcattgatag
     2161 cacattctat ccctttttgc gggtcaatcc tggaaaggca caggagcgta tcgttttttc
     2221 tctttattcg ccttctggtt ttataactgg aaagatcaat cccattatag acaacatctt
     2281 tgtaagacaa tcccgcatca tcatgtatcg tctgtgattt gctaatcgca acaaaactat
     2341 ggtgttttct gttttttgca gatctgtata gcattatatt ttcccaaccc atgtctttag
     2401 cgctcatact attgtgtatt gtgctgagca cctgaactcc tgatccattt aatgaagaag
     2461 ttacaagcgg atcgccatat acatgagaat gtataacttc aaattcccct tcaacttcta
     2521 acgctttgcg taagcccaga aaattgtcag ttatatcatt ccaggaaatg ctaattttac
     2581 tgtcctgccc atttttaata tcctgtccaa gtacagaaca tggatacgtt ggtaccagtt
     2641 ttgccgaagt tattgaatct cccgtggcaa atagggttac atcatgcccc cattccacta
     2701 acttttcgca tatccaataa acaatttttt ccgctccgcc atattttgat tcatgcggag
     2761 gagctatcct ataccagact gaaggcgcaa ctattgcaat tttcattttg tttccaactc
     2821 aactattgta ttctttatct cgttacttct tacaactaaa ctccctggtt ccctgtatgt
     2881 aactttacat atttatacaa cataggtaaa actatatatc agggttgtga agacatgaag
     2941 ttatgcgcat tgccatgttt tgcagtagtt tgctgcctac gcctccggca aacaaagaaa
     3001 aaagaattca tgccccgctt catcttaccg ctcttctagc ctctgagatg gcaaaacgcg
     3061 gtcactctgt gacacttttt ggcgctaagg gcacaaaatc aaaagcaaat gtggttgatt
     3121 tggatctacc gccgctgttt aaccatccac aaataggtga ttcaatagat ttgaagaacc
     3181 acagcgctat taatttttat gagcaggtat ttatctcgga aatttatcgt agagcggcag
     3241 aaggagaatt cgacattata catatccatc cggttgatct atccatcaac tttgcagtca
     3301 attgcagagt tcctacagtt tttaccttgc acgatcctat ttccccgtgg aggcgcttta
     3361 tttataatac gcacaaaatg aataaaaaca ttttttatgt atcgataagt gattcccaac
     3421 gcctgcccct taaagatata aattttatag atacgatcta taatggtatt gatctttgca
     3481 ggtatccgtt caatgctctg cccggaagtt atttgttatc agcatcaaga ttggttccgg
     3541 aaaagggggt cgatgtggcc atcagggccg caaagagaac aggtttacca ctgaaaataa
     3601 ccggagagcc cgcctccgat aataacagtt actggatgga aaaaataaaa cctgatgtag
     3661 gaggcaagat cagttatgaa ggaatggttt ccacacggcg catgttcagt atatataaaa
     3721 atgctgccgc ccttcttttt ccgataaaat gggaagaacc attcggcttg actatgatcg
     3781 aggctatggc ttgcggcaca cccgtgatcg cttttaaccg gggttcagtc aaggagattg
     3841 tagtggatgg caggacaggt tttatagttg atgacctgaa cgggatgatc cggtcaataa
     3901 agaaaattaa ctctatcgac cggatggaat gtcggcgtca tgtcgaagag aacttcagca
     3961 tgaaaaaaat ggtggataaa tatgagaagg tatatgagaa aatcttattc aaatctcaaa
     4021 ttcccaggag tcgataatat atttttcata cttatcggcc attattttgg ccgtgaagtt
     4081 ttcctcaacc cattttctgc ataccgctgg atcaatgcgt ttgcttattt ctttaattct
     4141 tatacacatt ttgtgcctat cgtttacggg cactaagaaa attttccgct ctcatgcacc
     4201 tgagattaaa gtcttcccac catgtggttt ctcagatttc aacagacggg ggcggcattc
     4261 ttttatgctc actcaatgaa acgcgcctgg ccagtt
//
LOCUS       DATGBN010000078         5913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_122436, whole genome shotgun sequence.
ACCESSION   DATGBN010000078 DATGBN010000000
VERSION     DATGBN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122436"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1633)
                     /locus_tag="VKL21_01700"
     CDS             complement(101..1633)
                     /locus_tag="VKL21_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HMB44514.1"
                     /translation="MEEILTPSIFCENQANNYIKITNSDYRKKKGQFFTPPKIAKFMA
                     EISYERGSQTLKILDPGAGAGILTCAICELMASKDNIKVIEVDAYENDTNLANYLQES
                     FNFCQQWLNRFNIKFYYKIIEKDFILDAIEKFRTNKFSTYDIIIGNPPYFKISKNNPQ
                     AQEAIEFVYGQPNIYSLFMGVAVNLLNDDGQMVFITPRSYTTGPYFKAFRKIFFGKMK
                     PERVHIFESRKDTFHKDSVLQENIILKARKSNNSKNIEISLSTGINDIEKSPIHLAPL
                     NHVLFQRNGDILFRLPVDEQDDSILIKVDEWTGSLQKFGMKISTGPVVPFRTTEFLSS
                     SNIENCDQLAPLLWMTNVYAMKVLWPKNGNNGNGKPQFIIENTQTCNRKLLVKDSNYV
                     LLHRFSAKEQHRRLTAAPLLKGKLKSEKIGIENHLNYIYRSDGTLTDTETLGLAAIFN
                     SSLLDRYFRISNGNTQVSATEIRAMPLPSLTDITEIGESLVKLSHTPTLEELDKMVWS
                     KV"
     gene            complement(1851..4478)
                     /locus_tag="VKL21_01705"
     CDS             complement(1851..4478)
                     /locus_tag="VKL21_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB44515.1"
                     /translation="MVVNKGLKFRKIDLHIHTPASKCFKNKNTEDDAVIKKSIEKGLD
                     AIAITDHNSAECIDVIKDKAKNTSLVIFPGVEITTSEGIHVVALFDVNKSKADIENFL
                     GAIDITPDKYGATDAISSFNAQQIMEKIVERGGLAILAHIDGSKGAFKELSGNPRLKL
                     FNEAEYVAVECKGKTLPSDLKKEKGFKRIPTFYIASDNPDPEDPKKHSIDGIGNKFSY
                     FKMDVIDIEGLRQCFSDPKVRIMLTDEIPEYNYPRILSLRVNDGFLKDQQFEFVPGLN
                     CIIGGKGTGKSLMIEFLRFGLDQSSSDATILEDHSSKLEKQLGIGQKVIVAFQLENGS
                     KYEVVRSYEGFENEKIICGSECKNIVTRENYQGDIATLFPILAYSQLEVIKIAEREEA
                     QLELIDKFIETVTFEDRINDLTTSLNLNDENLSKSIQAKVQVASLDVEISTIDTQIKN
                     IDELLKNLILTELKLLEKKDMILNNNLSHVTNIQTQIKSAKETINKLAFEEIDEEFTK
                     DNEITDASNIAFKTKAFAVEKLENILSEISPLSCSINGYISICQSNLEVKRREHEELL
                     KEKGIDKNIENNRRQLVKRMKKLEIEKAKYQSLVDEYEVLSSERHSLLDSLDNVREEY
                     YNKRKEKYEELTQRSHQKLKLEISHKANRSSFKDELKALLKGSKLREQVIEQVVGALL
                     PMDFIELILTRNIEELTSRASIAQEPSRKIVEKLWSIEDLKDILKVQHACYPEDSPSI
                     RFQKEDGGYYPLKELSIGQKCTALLIIALLEGKMPAVIDQPEDALDVASVWKDITLKL
                     RGNKENRQFILTTHNSSVAVASDSDKFIVLDAGAQRGGVRATGAIDRNDVKKEVLEHL
                     EGGNEPYLIRQKKYNITTG"
     gene            complement(4575..4811)
                     /locus_tag="VKL21_01710"
     CDS             complement(4575..4811)
                     /locus_tag="VKL21_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44516.1"
                     /translation="MKGSFIFANKAGNSWLWKASKEERIEMAKQIASTVETTDEEISY
                     IDTANLQKMHKEERMWIAKKIVSTIPHDWGYTSC"
     gene            complement(4817..>5913)
                     /gene="nrdD"
                     /locus_tag="VKL21_01715"
     CDS             complement(4817..>5913)
                     /gene="nrdD"
                     /locus_tag="VKL21_01715"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015313753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HMB44517.1"
                     /translation="FDNQLPEYRGAGKGISCYQCCAYNFSSSHDKDSEFEDKLYFRDG
                     KHFSMGAWQVVSLNCPRAAYKAKKDDEALFRELKNMMDLCMELFKVKRKWMDIVIGNG
                     RMPFVTQRPKDPFTMTHGQIAVDTKGLVYTIGVIGINEMVQHHIGKQIHESEEARRLA
                     IRAMFEMKFYAKELSQKYGMEIALARTPAETTAQRFAVSDILHKEYRKCAEPIIKGNV
                     PEALKHISETRDLPIYYTNGTHIPPNADVSLPQRIKLEETFFPIVDGGNILHIWLGEA
                     APDPHGLKEFAMNLAKNTQVGYFAFTKDMTVCMDDFHVASGLLDECPNCESENVEHLS
                     RVTGYIQAVSGWNEGKKQELKDRKRYGVGA"
BASE COUNT         1577 a         1231 c          967 g         2138 t
ORIGIN      
        1 ttgtcttgct ggaagtccta tagacctcaa aatatcttgg gcctcttcaa tttttttcaa
       61 ctttaaaatc tcctaacttt attaaaacta ttttcataga tcatactttt gaccaaacca
      121 ttttgtcgag ttcttcgagt gttggtgtat gagatagctt tactagactc tctccaattt
      181 ctgtaatatc agtgagggat ggaaggggca ttgctctaat ctctgtagca cttacttgag
      241 tgtttccatt ggatatacga aaatatctat ctaaaaggga actattaaaa attgctgcca
      301 atcctaatgt ttcagtatca gttaatgttc catccgaacg ataaatataa ttcaagtgat
      361 tttctattcc aattttctcg gattttaatt ttccctttaa gagaggagct gctgttaatc
      421 tgcgatgttg ctcttttgca ctaaatcggt gtagaagcac ataattactg tctttgacga
      481 gaagttttct gttacaagtt tgagtgtttt ctattataaa ttgcggcttt ccatttccat
      541 tattaccatt ttttggccat aatactttca ttgcataaac atttgtcatc caaagtaagg
      601 gtgctaactg atcacaattt tcaatattgg atgatgataa aaattcagta gttctaaatg
      661 gaacgactgg tccagtagat attttcatac caaatttttg taaacttcca gtccattcat
      721 ccacttttat taatatgcta tcatcttgct catcaactgg aagtctaaat aaaatgtctc
      781 catttcgctg gaataaaaca tgattcagag gagcaagatg gataggggat ttctcgatat
      841 cattaatacc agtacttaaa gatatttcaa tatttttaga gttgttactc tttcgtgctt
      901 taagtatgat attttcttgt aatacggagt ctttgtgaaa tgtatctttt cttgattcaa
      961 aaatatgtac tctttctggc ttcattttcc caaagaagat ttttctaaat gctttaaaat
     1021 atgggcctgt tgtatagctc cgaggagtga taaaaaccat ttgaccatca tcattcaaca
     1081 agttaactgc cacacccata aaaagcgaat atatatttgg ttgtccataa acgaactcaa
     1141 tcgcttcttg agcttgaggg ttatttttgg atattttaaa atatggagga tttcctatta
     1201 taatatcata agtcgaaaat ttattcgttc tgaatttttc aattgcatcc aaaataaaat
     1261 ctttttcaat tatcttataa taaaatttaa tattaaagcg atttagccat tgttggcaga
     1321 aattaaatga ttcttgcaaa taattcgcta aatttgtgtc attttcgtat gcatctactt
     1381 ctataacctt aatattgtcc tttgaagcca taagctcaca gattgcacat gttaagatac
     1441 ctgcacctgc accaggatca agaattttta aagtttgcga acccctttca tatgatattt
     1501 ctgccataaa tttggcaatt ttaggcggtg taaagaattg tccttttttc tttctataat
     1561 ctgaatttgt tatttttata taattatttg cttgattctc acaaaaaata cttggagtaa
     1621 gaatttcttc catagtttca aaattgttgt tagtcataat ttattaataa tttagtcaat
     1681 aattcataat ttatttcctt gtatttgcat cgtggcgggc atgatgccga tgaatggaga
     1741 tttattgccc cagagatgtg ctgtaactgg cgtggcacag ccatgtcctg aaaagtctgc
     1801 aatgctgaga aagtgttaga acctgttgaa gtttgtttaa tgctcattat ctatccagtt
     1861 gtaatattgt attttttctg tcgtattaga tacggttcgt tgccgccttc caagtgctcc
     1921 agaacctctt ttttaacatc gtttcggtct atggcgccag tagctctaac tccgcctctc
     1981 tgagcacctg catcaagcac tataaattta tcggaatcag aggctactgc aacacttgaa
     2041 ttatgagtgg ttagtatgaa ttgtctattt tctttattcc cgcgcaattt taatgttata
     2101 tctttccaca ctgatgccac gtctaaagca tcttctggtt gatctatcac cgctggcatt
     2161 ttcccttcta aaagagctat tattaggagg gcggtacact tttgccctat cgataattct
     2221 tttaatggat aatatccacc atcttctttt tgaaacctta tagaaggcga atcttcagga
     2281 tagcatgcgt gttgtacctt caagatatct ttaagatctt cgatgctcca tagtttctca
     2341 acaattttcc tggaaggttc ttgagctata cttgctctgg aggtaagctc ctcgatattt
     2401 cttgtcaaaa tcaattctat gaaatccatc ggtaataatg cgccaacaac ctgctcgata
     2461 acttgttccc ttaatttcga accctttaat aatgctttta gttcgtcctt aaacgaactg
     2521 cgattagcct tatgcgatat ttccagtttc agtttttgat gtgagcgctg agtcaattct
     2581 tcatattttt cttttcgctt attataatat tcttcacgaa cattatccaa cgaatccaat
     2641 aaagaatgtc tctcagaaga taaaacttca tattcatcaa ccagagattg atacttcgct
     2701 ttttcaatct ccaacttttt catcctcttt acgagttgtc ttctattatt ttcaatattt
     2761 ttatctatcc ccttttcttt taggagttct tcatgttctc tccttttcac ttctagatta
     2821 ctctggcaaa tagatatgta gccatttata gaacagctaa gtggcgatat ttctgaaaga
     2881 atattttcaa gtttttcgac agcaaatgcc ttggttttaa aggcaatatt tgaagcatca
     2941 gtaatttcat tatcttttgt aaattcttca tcaatttctt caaaagctag tttgtttatg
     3001 gtctcttttg cactttttat ttgggtttga atattggtga catgagataa attattattt
     3061 aaaatcatat cctttttttc tagtaatttt aattctgtta aaataagatt ttttaatagc
     3121 tcatcaatat ttttaatttg agtgtcaatt gtacttattt caacatctaa agaggcaacc
     3181 tgtactttag cttgtatgct tttagaaaga ttctcatcat ttagattcaa acttgtagtt
     3241 aagtcattta ttctgtcctc aaatgtgaca gtttctataa atttatcaat gagttctaat
     3301 tgtgcttcct ccctttctgc tatttttata acttctaact ggctgtaagc taaaatagga
     3361 aataatgttg ctatatcgcc ttggtaattt tctcttgtca caatattttt acattcagag
     3421 ccacatataa ttttttcgtt ttcaaatcct tcgtatgatc ttacgacctc gtatttagat
     3481 ccattctcaa gttgaaaagc aactataact ttttgaccta ttcctaattg tttttcaagt
     3541 ttactagaat gatcttccaa aatggtagca tcagatgatg attggtctaa gccaaagcgc
     3601 aaaaattcaa tcatcagaga ctttcctgta ccttttccgc ctattataca attcaatcct
     3661 ggaacgaatt caaactgctg gtctttcaaa aaaccatcat taactcttaa actcaaaatt
     3721 cgtggataat tatattctgg tatttcatcc gtcagcatta ttcgcacttt gggatctgaa
     3781 aaacattgtc ttaacccctc tatatcgata acatccattt tgaaatacga gaatttgttt
     3841 ccaattccat caatagagtg tttttttgga tcctcagggt caggattatc agaggctatg
     3901 tagaaggttg ggattctctt aaaacctttt tcttttttta gatcagatgg caaggtttta
     3961 cctttgcatt caactgcaac atattcagct tcattaaata gctttagcct tggattgcca
     4021 ctgagttctt taaaagcccc tttagaacca tctatatgag ctaatatggc taggccacca
     4081 cgttctacaa ttttttccat tatttgctgt gcattgaatg atgaaattgc gtccgttgcg
     4141 ccatatttat caggagtaat gtcaatagct cctaagaaat tttcaatatc agctttagat
     4201 ttattaacat cgaataatgc tacgacatgt atgccctctg atgttgttat ttcaactcca
     4261 gggaaaataa ctaaagatgt attttttgct ttatctttaa tgacatcaat acactctgct
     4321 gaattatgat ctgtaatagc aattgcatct aacccctttt caatagattt cttaattaca
     4381 gcatcatcct ctgtattttt attcttaaaa cattttgaag ctggagtatg tatatgaaga
     4441 tcgatctttc taaattttaa acctttattt accaccataa cctatcctcc tggttatttt
     4501 attctctcta ctatctgaca tccccccacc ccatacccct tcctgtgtcc ttcagcttct
     4561 gcatattcta tcccctaaca gctcgtatat ccccaatcat gagggatagt cgacactatt
     4621 ttcttagcga tccacatcct ttcctcttta tgcatcttct gcaggtttgc cgtatcgata
     4681 taagatattt cttcatcggt tgtttcaacc gtggatgcta tctgctttgc catttctatc
     4741 cgttcttcct ttgaggcttt ccataaccat gaattcccgg ctttattggc aaagatgaag
     4801 gatcccttca tctccatcac gcacccaccc catatctctt cctgtcctta agctcctgct
     4861 tcttcccctc attccaccct gagacagcct ggatataccc tgtcaccctg ctcagatgct
     4921 ccacattctc gctctcgcag ttggggcact catcaagcaa acctgatgcc acatggaaat
     4981 catccatgca caccgtcatg tccttggtga aggcaaaata gcccacctgc gtattcttgg
     5041 caaggttcat ggcaaattcc ttaaggccat gcgggtcagg cgccgcttca ccgagccaga
     5101 tatgaaggat attgcctcca tccactatgg gaaagaaggt ctcttcaagc ttgatgcgct
     5161 gagggaggga gacatccgcg ttgggcggga tgtgcgtgcc attggtgtaa tagataggca
     5221 aatcccttgt ttccgagatg tgctttaatg cttctggaac attcccttta attatgggct
     5281 ctgcgcactt cctgtattcc ttgtgcagga tatctgatac tgcgaatctt tgcgctgtgg
     5341 tctcggccgg cgttcttgca agcgctattt ccataccgta cttctgggat aattcctttg
     5401 catagaactt catctcgaac atggcgcgga tagccaggcg tcttgcttcc tcgctctcat
     5461 gtatctgctt tccgatatgg tgctgaacca tctcgttaat tcctatgacc ccgattgtat
     5521 agacaagccc ctttgtatcc actgcaatct gcccatgagt cattgtgaag gggtctttgg
     5581 gtctttgcgt aacgaatggc atccttccgt tcccgatcac gatatccatc cacttcctct
     5641 tcaccttgaa gagctccatg cacagatcca tcatgttctt caattcccgg aagagcgctt
     5701 cgtcgtcctt ctttgctttg taggctgcgc gcgggcagtt gagggacact acctgccagg
     5761 cgcccatcga gaaatgcttt ccgtcacgga aatacagctt gtcctcgaac tcgctgtcct
     5821 tatcgtgcga tgaagagaaa ttataggcac agcactggta gcagcttatg ccctttcccg
     5881 cccccctgta ttcgggaagc tggttatcga aat
//
LOCUS       DATGBN010000079         5086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_161134, whole genome shotgun sequence.
ACCESSION   DATGBN010000079 DATGBN010000000
VERSION     DATGBN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161134"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VKL21_01720"
     CDS             complement(<1..382)
                     /locus_tag="VKL21_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMB44518.1"
                     /translation="MPGFELIGNEEFEQVKEVFDKSRILFRHSFDNLRNGVYKVRDFE
                     NAFADHMGVKHALAVTSGTSALRVGLAALGIKQGDEVITQSFTFVATVEAIIESGATP
                     IITEIDKTLNMDPDDLEMKICTKTK"
     gene            complement(454..648)
                     /locus_tag="VKL21_01725"
     CDS             complement(454..648)
                     /locus_tag="VKL21_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44519.1"
                     /translation="MAEYHKYVFDAEKREFAGKFEEMYKAEKERYPDINFNVADQTSD
                     GWDDHPIFKDKNYDCIVTMD"
     gene            complement(658..1821)
                     /locus_tag="VKL21_01730"
     CDS             complement(658..1821)
                     /locus_tag="VKL21_01730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HMB44520.1"
                     /translation="MFEFYFNTKVFYGANSCISAILTILKNESWNKIGIVIDYNVSKT
                     EAVNELVFAIRNNCDKTIIDYCPISEPTYDFLDKVRTTFENQDLQAVVGIGGGSSLDT
                     AKAIAVLVNNRNPAISYRGFNKMTEPVLPVVAIPTTAGTGSEVTPNASFVDTIEKRKM
                     GINGEAVRPRYAILDPGLTLSCPERPTISAGVDSIVHAVEAYTAKKTNPLARMFAKEG
                     LSYVLPNLSLVLKEPQNINYRERVMNGAFLSGIALMNSGTGPAAAMSYPLGVHFKVPH
                     GIAGGVFLPHVIRYNIEMGYYQYADIYDCITDRDVSHKYNEQEKAQLFLKKILSLWKV
                     LSIPDDLVKLGLKTDFMPQFIRETMELKGALDQNPVPFYEDGIVSVLKQLKVT"
     gene            complement(1814..2761)
                     /locus_tag="VKL21_01735"
     CDS             complement(1814..2761)
                     /locus_tag="VKL21_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMB44521.1"
                     /translation="MNVLITGGLGQVGSYLTEELAKEKSNTITVLDNFSSNIKDFKVP
                     EGVIIVKGDIRNNEVANKLVTENEIIIHTAAQVSVVNSIDNPVYDAENNIMGTLTLLN
                     AARKSNISRFLYISSAAVYGAPQYLPIDEKHPVNPMSPYGLSKLTGEKYAMMFHLLYG
                     IPVVCLRPFNIYSPRQNPGSPYSGVITKFIKKVKNNQNPMIFGDGNQTRDFVYIGDVI
                     EAISKTMENKKAVGEVFNIGTGKPTRIKELAEMIIKVSNKKLESEFAPSSVGDIGESY
                     ADITKIRKTLGYRPKYLLEDGLNLCLSEIGKVNKENHYV"
     gene            complement(2763..3890)
                     /locus_tag="VKL21_01740"
     CDS             complement(2763..3890)
                     /locus_tag="VKL21_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB44522.1"
                     /translation="MKIFYLTNGENVHDERFLKKMIKEGYEIFLISYSKDKIPKETPG
                     LKKVIHKPLVPSFIPFSIYFNYFPFGFLHLKYLIWKYKPDILHAGWVQHYGFISALSG
                     FHPLVIIPWGSDILIEPHKSFLHRMLTKYTLNRADMISVDAEFVKDEIIRISGYPDEK
                     IVVFPQGIDQKMFYKKENGSGIRKKLGWSDKKIMIMARTFKPVYGIEYFLSAMTEVVK
                     KVPDVRVLMCGDGPLRDEFKSFLAKNSLSEYFYFAGMVKNDDLPKYYNAADIYVSTSL
                     SDGTSLALLESMACGLPSVVTDVPAILEWVKDGENGFIVPRKNSEVLAEKLIELLKND
                     AMRREFGRKNLAIAKERANWDDNFMKLVYIYDKLCPQKEVN"
     gene            complement(3925..4623)
                     /locus_tag="VKL21_01745"
     CDS             complement(3925..4623)
                     /locus_tag="VKL21_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB44523.1"
                     /translation="MKKTFRDDYSKFWLKNAECRYGFSQYHKDIIDLLTEILAKSENC
                     DIQKILEVAIGTGYPIAVELDKMEFNISGIDLSLDLLHKMKLNSRKITGIVSDSEMLP
                     FKDHCFFLTYCIQSSWYFPNIEKAISEMLRVTKDDGYIVIDVMNKFSPYIQYRSKGYR
                     IFKYFQRLYTKIFNKNEPTKYFHNYTVNVFKIYRIFRQSSKNISLTTSSKIKNLGFSK
                     FDWFNYRVIYIAKK"
     gene            complement(4653..>5086)
                     /locus_tag="VKL21_01750"
     CDS             complement(4653..>5086)
                     /locus_tag="VKL21_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMB44524.1"
                     /translation="EKYRNYGKEVIGGKVYYPLRNGFNYRLSEFVAALGIVQLERLPV
                     ILEWKRELASKFDNIFQNRIKFPEGMVSGYYKYIVFDYKLKEETGKVFGMNDLCHIID
                     GTNISLPNSEWIAQHHNCVPIWYGWGGKEMDIEQIKLRLLG"
BASE COUNT         1502 a         1052 c          797 g         1735 t
ORIGIN      
        1 ctttagtttt agtacagatc ttcatttcaa gatcatccgg atccatattc aatgtcttat
       61 ctatttcagt tatgatgggc gtagctccgg attcaattat ggcttctaca gtagccacaa
      121 aggtgaaact ctgggtaatg acttcgtccc cttgtttgat acctaaagca gctaatccca
      181 ctctcaaagc tgaagttccg gaagtcacag ccagagcgtg tttaacaccc atgtggtcag
      241 caaatgcatt ttcaaaatcc cgaactttat atacaccatt acgcaaatta tcgaatgaat
      301 gtctaaatag aatcctgctc ttgtcaaaaa cttctttaac ttgctcaaat tcttcatttc
      361 cgataagttc aaatcctggc attttagtct cctttatttt tactttagtt gataactttc
      421 taattacatc tttccagttt ccataatatg aaattaatcc atagtaacta tacaatcata
      481 gtttttgtct ttaaaaatag ggtgatcatc ccaaccgtct gatgtctgat ctgctacatt
      541 aaagttaata tcaggatacc tttccttttc agctttatac atctcttcaa acttacctgc
      601 aaattctctc ttttcagcat caaaaacgta tttatgatat tctgccataa aaatatccta
      661 tgtgactttt agctgcttaa gaacagatac tatcccatct tcatagaagg gaacaggatt
      721 ctgatccagc gcacctttta attccattgt ctcccgaata aattggggca taaaatcagt
      781 cttaagtcca agctttacaa ggtcatcagg tattgataaa actttccata gcgagagtat
      841 ttttttaaga aagagttgag ccttttcctg ttcattatat ttatgagaaa catccctatc
      901 agttatacaa tcataaatat cggcgtattg gtaatagccc atttcgatat tatacctgat
      961 tacatgcgga agaaaaaccc ctccggcgat cccatgaggc actttaaagt gaacgccaag
     1021 cggatatgac attgctgcag caggccctgt tcctgaattc atcagggcaa tacccgataa
     1081 aaatgctcca ttcatgaccc tctcccggta attaatattt tgcggctctt ttaaaacaag
     1141 agatagatta ggtaatacat agcttaatcc ttctttagcg aacattcgtg ccagaggatt
     1201 agtctttttt gcagtatacg cctcgacagc gtgaacaata ctatcaactc cggctgatat
     1261 ggtcggtctt tccggacacg agagtgtcaa tcccggatca agaattgcat atcttggcct
     1321 taccgcctct ccgttaattc ccatcttacg cttctcaatg gtgtctacaa atgatgcatt
     1381 gggagtgacc tcacttcctg tacccgcagt ggtagggatg gcaacaacag gtaggactgg
     1441 ttctgtcatt ttgttaaatc ccctatagga tatagcagga tttctgttgt ttaccagtac
     1501 agctattgcc ttagcagtgt caagactgct gccgccaccg atccccacca cagcttgaag
     1561 atcttgattc tcaaaggtgg ttctgacttt atccaagaaa tcatatgtgg gttcagatat
     1621 gggacagtaa tcaataatag ttttatcaca attatttcta attgcgaaaa caagttcatt
     1681 cactgcctcg gtcttagata cattataatc aatcacaata ccgattttgt tccaggattc
     1741 attttttaag atcgttaaaa tcgctgagat gcaactgttg gccccataaa aaactttagt
     1801 attaaagtaa aattcaaaca taatgatttt ccttgttaac cttacctatt tcacttaagc
     1861 ataaattaag tccatcttct aatagatatt taggtcggta tcccagggtt tttcttatct
     1921 ttgttatatc cgcatagctt tcccctatat ctccaaccga agagggcgca aactctgatt
     1981 ccagtttttt atttgaaact ttgattatca tttccgccag ctctttaatt cgagtaggtt
     2041 ttccggttcc tatattgaac acttctccaa ccgctttctt gttctccatc gttttagata
     2101 tagcttctat aacatcccca atatatacaa agtctctggt ttggtttcca tccccaaaga
     2161 tcattggatt ctggttattt tttacctttt tgatgaactt tgtgatcact cccgaatatg
     2221 gacttccagg attctgccgt ggactgtata tattaaaagg gcgcaggcaa acgacaggaa
     2281 tcccgtatag taaatggaac atcatagcat acttctcccc tgtaagctta ctcaaaccat
     2341 atggcgacat cggattaaca ggatgttttt catcaattgg caaatattgc ggtgccccat
     2401 aaaccgcggc tgaactaata tagaggaatc tgctaatatt gctttttctt gccgcgttga
     2461 ggagtgtcaa tgttcccatg atattatttt ccgcatcata aaccggattg tctattgaat
     2521 ttacaacact gacctgtgct gcggtatgaa ttattatttc attttcagtg acaagtttat
     2581 ttgcaacctc gttattcctg atatctcctt ttacaataat aactccttca ggaactttaa
     2641 aatccttaat attacttgaa aaattatcca aaactgtgat agtattgctc ttttcctttg
     2701 cgagttcttc cgtcagataa gaacctacct gccccaaacc acctgttatt aagacattca
     2761 attcagttca cctctttttg tggacagagt ttatcatata tatagacaag tttcatgaaa
     2821 ttatcatccc agtttgcccg ctcttttgcg atcgctaggt ttttcctgcc gaattccctg
     2881 cgcatagcat cattcttgag cagttctatg agtttttctg caaggacttc tgaatttttc
     2941 cttggaacaa taaaaccgtt ctctccatct ttcacccatt caagtatagc ggggacatcc
     3001 gtaactacag aaggaagacc gcaagccatg gactcaagca gagccagcga agtgccgtca
     3061 ctcagtgagg tggaaacata aatatcagca gcgttatagt attttggcag gtcatcattt
     3121 ttcaccatac ctgcgaaata gaaatattca ctaagtgaat tttttgcaag aaaagactta
     3181 aactcatccc ttagagggcc atcgccgcac attaaaacgc gcacatcagg tacttttttt
     3241 actacttcgg tcattgccga gaggaaatat tctataccat atacaggctt gaatgtcctt
     3301 gccattatca ttattttttt atcgctccac cccagctttt ttcgaattcc cgaaccattt
     3361 tcttttttat agaacatttt ctggtctata ccttgtggga aaaccactat tttttcatcc
     3421 ggatatccac ttatgcggat aatttcatcc ttcacgaact ccgcatcaac tgagatcata
     3481 tcggcccggt tcagcgtata ttttgtaagc atcctgtgta aaaatgattt gtgtggctct
     3541 atcaaaatat ccgaacccca cgggatgatg acaagcggat gaaaaccgga gagagccgaa
     3601 ataaaaccat agtgctgcac ccatccggca tgaaggatgt ctggcttgta tttccatatc
     3661 aaatacttga ggtgcaagaa tccaaatggg aaatagttga agtatatcga aaacggaatg
     3721 aaagaaggga caagaggttt atgaatcact tttttcagcc cgggtgtttc tttgggtatc
     3781 ttatcctttg aataacttat caagaatatc tcgtagccct cttttatcat ctttttgaga
     3841 aatctttcat catgaacgtt ttctccattt gttaggtaaa atattttcaa gtgatcaaac
     3901 ccttttcaat aaacaccaaa tatattattt ttttgcaata tatattactc tataattgaa
     3961 ccagtcaaat tttgaaaatc caagattttt tatttttgag gatgtggtta gacttatatt
     4021 tttacttgat tgtctaaaaa tcctgtagat tttaaaaaca ttaactgtat aattgtgaaa
     4081 atattttgtt ggctcatttt tattaaaaat tttagtatat aatctctgaa aatatttgaa
     4141 aatacgataa ccttttgaac gatattgaat gtaagggctg aatttgttca taacatcgat
     4201 tactatatac ccatcatctt tagtaactcg gagcatctca cttattgctt tttcgatgtt
     4261 tgggaaatac catgaggatt gaatgcaata tgtcagaaaa aaacaatgat ctttaaatgg
     4321 caacatttct gaatcactaa caatacctgt aatttttctt gaatttagtt tcattttatg
     4381 taataagtcc aaagagagat cgatgccaga tatattaaat tccattttat caagttctac
     4441 agcaatcgga taacctgttc ctatggcaac ttctaaaatt ttttggatgt cgcaattttc
     4501 tgatttcgcc aatatttcag tcaaaaggtc gataatatct ttatgatatt gagaaaaacc
     4561 ataacgacac tctgcgtttt ttaaccaaaa tttagaataa tcatctcgga aagttttttt
     4621 catttccgtc tgagtattac ttataataat aatcaaccca ataatctcaa tttaatctgt
     4681 tcgatatcca tttctttacc tccccatcca taccatatag gtacacaatt atgatgttgt
     4741 gcaatccatt cactatttgg tagggatata tttgttccat caatgatatg acagaggtcg
     4801 ttcatgccaa aaacctttcc agtttcttct tttaatttat aatcaaaaac aatatattta
     4861 tagtaacccg ataccatacc ttctggaaac tttattctat tttgaaaaat attatcaaat
     4921 ttagaagcta attctctttt ccattcaagg attacaggca gcctttctaa ttggacaata
     4981 cctagagctg caacaaattc gcttaatcta taattaaatc catttcttaa tggataatat
     5041 acttttccac ctattacttc ctttccataa ttcctatatt tttcaa
//
LOCUS       DATGBN010000080         6438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_161202, whole genome shotgun sequence.
ACCESSION   DATGBN010000080 DATGBN010000000
VERSION     DATGBN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161202"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VKL21_01755"
     CDS             <1..182
                     /locus_tag="VKL21_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44525.1"
                     /translation="TYKDLIEAGIIDPVKVVRTALQNAASIAGLMLTTEALVAEIPEK
                     KSDMPSMPGPESYMM"
     gene            complement(234..851)
                     /locus_tag="VKL21_01760"
     CDS             complement(234..851)
                     /locus_tag="VKL21_01760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB44526.1"
                     /translation="MKEHWNEIYEALDADELTWYEEIPAPSIKLLSKCHINKDETILD
                     VGAGASTFIDYLVNQGFKNIIAADISEIALNKLKERLGKEKESAVRWIVDDITCPIQI
                     QNLRDIAVWHDRAVLHFLLEEEEQQMYLSTLKKVIKKSGYVIIAAFSLKGAKKCSSLD
                     VKNYDRDMLAKFLGEDFILIEYFDYVYYMPSGKPRQYIYTLFQKK"
     gene            complement(913..1446)
                     /locus_tag="VKL21_01765"
     CDS             complement(913..1446)
                     /locus_tag="VKL21_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB44527.1"
                     /translation="MKIIALADTHIKSGSITDALPSGLIKLIEQADIVIHAGDFVTRQ
                     AYDELAGICRLEAVHGNMDEMSLKNLLPERKIIEVCGLKIGIIHEAALSIQDTTGARY
                     MAKEMGVDVLVFGHIHKPVIDKSDLLLLCPGSPTAPRLSEPCVIELIIDAGNISGRII
                     TLEGTRCATMKAMTDNI"
     gene            complement(1443..2252)
                     /locus_tag="VKL21_01770"
     CDS             complement(1443..2252)
                     /locus_tag="VKL21_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44528.1"
                     /translation="MKELSKKEFEEQYSLWISSAARDIAFSLNRLKPSVIKEIIARNE
                     QYLDRPESVFGAIGLDEVKKLAPGNILFLSTDAVVFSQSIFAPFPGVFDYAVALNRRY
                     YIEGSWYSIITLNTTYIVEANETILKYAIFHELLQKEIYEENMRNGIRKFSPDEKRKI
                     SNDTLNKAIESSGITPNELMTEKELMLQISSNSPLIPKPFAETALYHYVEKNLGEFND
                     FREKSSTEKEDAIGKKLNADFKEWLDFSTDTYRKYLLELKKELNYMDYGYA"
     gene            complement(2306..2605)
                     /locus_tag="VKL21_01775"
     CDS             complement(2306..2605)
                     /locus_tag="VKL21_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44529.1"
                     /translation="MGTVSSKKRLEIIERDVIPSMFVGVLAKDDKWLEHTLKETLPLL
                     EERALRLARECKESGECAQDDPLVNETRIKALFEDARSKLGKEHLTREAHSRYSH"
     gene            2732..3475
                     /locus_tag="VKL21_01780"
     CDS             2732..3475
                     /locus_tag="VKL21_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMB44530.1"
                     /translation="MKDKIVRRICSILLGLAILVIIAGATSTYFESPPPNGIMTGSAD
                     KMKIKTIEVSGTGIVSMTPDEAIVYMGVQTKSADAVTSQKDNAARMEKVINALADAGI
                     AKDNMETSSYTMYPVKDNENGENITGYVVSNQLKVTIKDINKTGIVIDKAVKAGANEI
                     NSISFTLSDESQQKVREQALKNAVKAARLDADRLAGELGVIIKAPLQVSTGGGIITTP
                     TVEVKSLATPIMPAGVTVSAFVSVIYQFE"
     gene            complement(3543..4319)
                     /locus_tag="VKL21_01785"
     CDS             complement(3543..4319)
                     /locus_tag="VKL21_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44531.1"
                     /translation="MGQNGKLNLERVPTGIEGFDELCEKGLLRNRSYLLSGSTGTGKT
                     IFGIQYLYNGITKYGEKGIYIATEERPDQLRENVLEFGWDLEALENEGKLAIVDACSA
                     KIGIPSHEKYVDMRPFDIGSIVDQIIEIQNRIGAKRAVVDSTTSIGYHLLDSTKIRLE
                     LLKLSTTLEIIGLTSILTCELLEGAIENSRFGIEQFVAEGVIKLSTKQVDNIYLSNIT
                     IIKMRGSNHSKQVHPYEITSKGIKIGGKAKDFPTQLCFKV"
     gene            complement(4298..4420)
                     /locus_tag="VKL21_01790"
     CDS             complement(4298..4420)
                     /locus_tag="VKL21_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44532.1"
                     /translation="MVSIKDMSLSKKLLGGFGLVCLLLILDLFGSGVKWGKMES"
     gene            5006..5079
                     /locus_tag="VKL21_01795"
     tRNA            5006..5079
                     /locus_tag="VKL21_01795"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5040..5042,aa:Val,seq:gac)
     gene            5210..6391
                     /gene="ftsZ"
                     /locus_tag="VKL21_01800"
     CDS             5210..6391
                     /gene="ftsZ"
                     /locus_tag="VKL21_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB44533.1"
                     /translation="MVTDLKEFARTAKPSVAVVGLGGAGCNITTWIAEKGISGGTIIA
                     ANTDVNHLYVQKADKVILLGEKICKGHGCGGYPEMGAQATREAIEELKTELEGTNLVF
                     LVAGLGGGTGTGAIPVVAELTRSLNALTIGCVTIPFTIEMSRREKAREAIKLLAESCD
                     SVVIIDNSKLREVAGNLPLKEALNVANALVGAFVKNLTDTITQPSLVNLDYADLRAVM
                     ERGGISSIGIGEGDGTDRVIKAVSQAISTPLLDVTNITDCYGVLIHIVGGEDLTLEEV
                     AVTGELIMDKVPNTKRIIWGAKVDNSLTGKVRVMAVLTGVESPFVSGAMEKVKEVEPE
                     PPKPEPKPEPEPPKPRPVPKIQTPRPQPKIEEEKSGNDYIWYAIILLIVLVMLWYFFL
                     R"
BASE COUNT         1936 a         1304 c         1276 g         1922 t
ORIGIN      
        1 atacatataa ggatttgata gaagcaggca ttatcgaccc cgtaaaagta gtgcggacag
       61 cgctccagaa tgcagcgagc atagcaggtt taatgctgac cacagaagcg cttgtggccg
      121 agatccctga aaagaagagc gatatgccct caatgccggg acctgaaagc tatatgatgt
      181 aattacatta cagtaagttg ccaatatggc ttcccaaagg aatccatatt gatttatttt
      241 ttttgaaata aggtatatat gtattgtcta ggcttacccg atggcatata ataaacataa
      301 tcaaaatatt caataaggat aaaatcctcc cccaggaatt ttgccagcat atcccggtca
      361 taatttttaa catccaggct gctgcatttt tttgctccct ttaaagaaaa ggcagcgata
      421 ataacataac cacttttttt gatcactttc tttagcgtgg ataggtacat ttgttgctct
      481 tcttcttcca ataagaaatg taaaactgcc ctatcatgcc acaccgcaat atctcttaga
      541 ttttggatct ggatcgggca ggtgatatca tctactatcc atcttactgc agattctttt
      601 tccttaccca gtctttcttt taacttgttc aaagctattt cacttatgtc agcagcaatg
      661 atatttttga atccctgatt aacaagataa tcaataaaag ttgacgctcc tgcccctaca
      721 tctaaaatgg tttcatcctt attaatatga catttagaaa gtaatttgat agaaggagca
      781 ggaatttctt cgtaccatgt cagctcatct gcatctaagg cctcataaat ttcattccag
      841 tgttctttca ttgagttatc catcttcagg agttatttgg attatgggaa tttaaaagta
      901 tttgatttaa attcatatat tgtcggtcat tgccttcata gtagcgcatc gcgttccttc
      961 aagggtgata attcttcctg aaatattacc tgcatcaata attaattcta tgacacaggg
     1021 ttcggataac ctgggcgcag tgggacttcc cggacacaga aggagcaaat cgcttttatc
     1081 gatcacaggc ttatggatat ggccgaacac gagaacatcc acacccattt cttttgccat
     1141 ataccttgcc cctgtggtat cctggataga tagtgccgct tcatgtatga tcccgatttt
     1201 caggccgcat acttcaatga tctttctttc gggaagcaaa ttctttaaac tcatctcatc
     1261 catgttcccg tgcaccgctt caagcctgca tatacctgcg agttcatcat atgcctgtct
     1321 tgtaacaaaa tctcccgcat ggataacaat atcagcctgt tctatcagtt taataagccc
     1381 tgaggggagc gcatcggtaa tggagcctga ttttatatga gtatcagcga gtgctattat
     1441 tttcatgcat atccgtaatc catataattc aattcttttt tgagttccag aaggtatttc
     1501 ctgtaagtat ctgttgaaaa atcaagccat tccttaaaat cggcattcaa tttctttcct
     1561 atggcatcct ctttttcagt actgctcttt tcgcgaaaat cattaaattc cccaagattc
     1621 ttttcaacat aatggtaaag cgccgtttca gcaaatggtt tcgggataag gggagaattg
     1681 gaggatattt gcagcatcag ttctttttct gtcattaact cattcggggt aatccctgag
     1741 ctttcaatcg ccttattcaa tgtatcattg cttatttttc gcttttcatc gggagaaaat
     1801 ttacgaatcc cgtttctcat attttcctcg tatatttctt tttgcaaaag ttcatgaaag
     1861 atcgcatatt tcaatatggt ttcattggct tccacaatat aagttgtatt gagcgttatt
     1921 attgaatacc atgaaccttc gatgtaataa cgcctgttca gggcgacagc ataatcaaaa
     1981 acaccgggga aaggtgcaaa gatgctctgg gagaaaacaa ctgcatccgt tgaaagaaat
     2041 aatatatttc caggtgccag ctttttgacc tcgtcaagac cgatcgcgcc aaacacggat
     2101 tccggtctat ccagatactg ctcgttgcgc gctatgattt cttttatgac ggatggcttt
     2161 aacctgttaa gcgaaaaggc aatatccctg gcagcagagc ttatccatag cgagtattgt
     2221 tcttcgaact ccttctttga aagctctttc atataacgta aatcgcaatt tcccaataaa
     2281 tagttagtgg aagggttttt tatttttaat gcgaatatct tgaatgcgct tcccttgtaa
     2341 gatgctcttt tccaagtttt gagcgggcat cttcaaataa agccttgatg cgtgtttcat
     2401 tcaccagcgg atcatcctgc gcgcattccc cgctttcttt acattcccgc gccagtcgaa
     2461 gtgcgcgctc ttcaagcagg ggaagtgtct cttttaatgt atgttccagc cacttgtcgt
     2521 cctttgcgag cacgccaacg aacatagagg ggatcacatc ccgttctatg atttcaagcc
     2581 ttttttttga agaaacagtt cccattgaaa actattctaa tttgttaaga tataaaactt
     2641 gggtcaacag gaaatttcat atacataagc agtgtttata ttattggtaa cagggagaat
     2701 taccaattta taaaataaag gagatgatat tttgaaagat aagatagtcc gaagaatatg
     2761 ttcaattttg cttggattgg ctatattggt gattattgcc ggcgcaacat ccacatattt
     2821 tgaatcaccc cctcccaatg gtataatgac tggaagcgca gataaaatga aaataaagac
     2881 aattgaggtt agcggaacgg gtatcgttag tatgacacct gatgaagcaa ttgtgtacat
     2941 gggagtgcag acaaaatctg ctgatgctgt tacctcacaa aaagataatg cagccagaat
     3001 ggaaaaagta atcaatgctc tggcagatgc cgggattgca aaagataata tggaaacatc
     3061 aagttacacc atgtacccgg tgaaggataa tgaaaacggt gaaaacataa ccggttatgt
     3121 agtttcaaac cagttgaaag tgacaataaa agatatcaac aaaaccggga ttgtgattga
     3181 taaggcagtg aaagcaggag ccaatgaaat taacagcata agttttaccc tctctgacga
     3241 gtcacagcaa aaagtcaggg aacaggccct gaagaatgca gtgaaggcag caagattaga
     3301 cgctgacaga cttgccggtg aacttggcgt gataataaaa gctcctctgc aggtgagcac
     3361 aggcggcggc atcatcacaa cacctactgt cgaagttaaa agtttagcaa cacctataat
     3421 gccagcgggc gtcactgttt cagcatttgt gtctgtgata tatcagtttg aatgataaaa
     3481 aacttaacta taaaaagaaa agggctgtga tagaaaaagg gattgtgccg ggagatgaaa
     3541 aatcagacct taaagcaaag ttgggtgggg aaatccttgg cttttccccc tatttttata
     3601 ccctttgatg ttatctcgta gggatgtacc tgtttagagt ggttagagcc cctcattttg
     3661 atgatagtta tatttgataa ataaatatta tcgacttgtt tagtactcaa cttaattact
     3721 ccctctgcaa caaattgttc aataccaaat ctactatttt caatagcacc ttctaataat
     3781 tcgcaagtaa gaattgatgt caatccaatt atttccaatg ttgttgataa ttttaacaat
     3841 tcaagtctaa tttttgttga gtccagcagg tgatatccaa ttgaagtggt tgagtcaacc
     3901 acagctcttt ttgcgccaat tctattctgt atttctatga tctggtcaac aatagaacca
     3961 atgtcaaaag gcctcatgtc aacatacttt tcatgggacg gtattcctat cttggcagaa
     4021 catgcatcta cgatggcaag ttttccttcg ttctctaagg cttccagatc ccatccgaat
     4081 tccaggacat tttccctcaa ctggtcagga cgctcttctg tcgctatata aatacctttt
     4141 tcaccatatt ttgtgatgcc attataaaga tactgaattc caaatatggt cttgcctgtt
     4201 ccagtagacc cggacaatag atatgatctg tttcttagta atcccttttc gcacaattcg
     4261 tcaaaacctt ctattccggt tggaaccctc tctaaattta actttccatt ttgccccatt
     4321 ttactcctga tccgaataag tctaatataa gcagaagaca gaccagaccg aatccgccca
     4381 gcagcttctt gcttaatgac atatctttta tacttaccat ttatgttatt cctccaatta
     4441 ttgggattca acctgaaaga tagccccttc agatgatttc tagataacag agaaatccac
     4501 ctgtttataa acggatttgt gaaaaaggtg tgaaatgaaa tgatttgggt ctgaatctat
     4561 agcagtcgcc cctgctattg atatgctaac ataatgataa tcatgtacac atataaatct
     4621 tatgcaaaac ttttcaaaaa agttatagtt ctcatgagct tattctttaa gtagttgctt
     4681 ttgtgcagcc atagcaagta ttgaattaac aagtttcttt ccctcttcaa tatcgtttac
     4741 cattcggata gtaatttttc cgctcgcgaa aaaatttact tcatgtgaaa acagaacatg
     4801 aattgctcca tgaaaggata aatataaaaa atatttatat ttttttggtt tcaaaaacaa
     4861 acgcttaata atgctccgat tcgaaccaca acttttaaac gttatagcgc tgtgataggg
     4921 ctacctcagt ggcagttgtt aaaaaagaga ttgtatagca ctacattcta aattcctaat
     4981 aacaatccac cagaggaaat aagcagggct cgtggtctag ttggttatga cgtcgccttg
     5041 acatggcgga gatccggagt tcgaatctcc gcgggcccat atttgtatta ttttctatgg
     5101 gttgtattct catcatgatg atgtgattat aatgaagtgt tgaaaaagca tatatagtaa
     5161 tatgacaata taaatagata gccattcata tgaaggaggc ttttataata tggttacgga
     5221 cttaaaagag tttgcaagaa cagcaaaacc atcagttgcc gtcgtaggct taggtggagc
     5281 gggttgtaat attacaacat ggatagcaga aaaaggtatt tcgggcggca cgataattgc
     5341 tgcaaatact gatgtgaatc acctttatgt tcaaaaagcg gataaagtga tcttacttgg
     5401 ggaaaaaatc tgcaaaggcc acggttgtgg aggttacccc gaaatggggg cacaggctac
     5461 cagagaggct attgaagagt taaaaactga acttgaaggc acaaaccttg tattccttgt
     5521 cgcaggattg ggcggcggaa caggaacagg cgcaatacca gttgttgccg agctcacacg
     5581 ctcgcttaac gcacttacca ttggctgtgt cacaattcca tttacaattg agatgtcaag
     5641 aagggagaaa gcaagagaag cgatcaaact actcgcagaa tcttgcgact ctgttgttat
     5701 catcgataac tcaaaattga gagaagttgc aggtaatctt ccattaaaag aggccctaaa
     5761 tgtcgctaat gcccttgtgg gggcatttgt aaagaatctt accgatacta tcacccagcc
     5821 aagccttgtg aaccttgact atgcagacct tcgtgctgtc atggaacgcg gtggtatctc
     5881 atccatcggc attggagaag gagatggcac ggacagggtc ataaaagccg tatcccaggc
     5941 catcagtaca ccactgcttg atgttacaaa tattaccgat tgttatggtg tgcttatcca
     6001 tattgttggc ggcgaagacc tgacattgga agaagttgct gtcacgggcg aattgatcat
     6061 ggataaagtg cccaatacca agaggatcat ctggggcgca aaagtagata atagccttac
     6121 aggaaaagta cgtgttatgg ccgtattgac aggtgttgaa agcccgttcg tatccggcgc
     6181 gatggaaaag gtcaaagaag ttgaacctga accgccaaaa ccggaaccaa aaccggaacc
     6241 tgaaccgcca aaacccaggc ctgtgccaaa gatccagacg ccaagaccac aaccaaaaat
     6301 tgaagaagaa aagagcggaa atgattatat ctggtatgca ataatactcc tgatagtcct
     6361 ggtaatgctc tggtactttt tcctgagata atccttttcg aaaaaatcaa cggactcgcc
     6421 gcgattcgaa cgcgggtc
//
LOCUS       DATGBN010000081         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_510837, whole genome shotgun sequence.
ACCESSION   DATGBN010000081 DATGBN010000000
VERSION     DATGBN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510837"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VKL21_01805"
     CDS             complement(<1..1051)
                     /locus_tag="VKL21_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HMB44534.1"
                     /translation="MVLILLIIVFSIIGTHIPQKSQLKSDVYNVWKTNHPTEAYYFEI
                     LGLTGLFSSPIFIGLAALLFINTFFCTRIMFNTAYRRLRYPQIQQKPYISGLDNNIEL
                     RTEKEQKYIISVINSVLKSGGYRIFQEENCIFAEKNRYGVFGTFLFHVCILIIIFAAV
                     YGSLGRMEGDMRLIEGQTLSEDHKNYVFVNEGPFFYDNHQKFDINLEKFYSDYNDETG
                     TLRDNAGKLVIKENGKEVKTDIVYSNNLMSYKGYTFMSNVYGLAPLLILKNQEGTVYS
                     GSYITATDSDSSGRYVASFEIGDMGLEGVLMVYMTVPLKSEITGSKDTKQIPILFLKI
                     FDKGNEIYDGMLRLND"
     gene            complement(1116..1574)
                     /locus_tag="VKL21_01810"
     CDS             complement(1116..1574)
                     /locus_tag="VKL21_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HMB44535.1"
                     /translation="MKPINKLISFIAVGSILFLAASWWENSQSEGDAAFIKLGGLSFH
                     TSIDTGINEAKNLSKPIFLYFRSETCYWCKKFEEEALSDKRVIDILNNNFVIVSIDTF
                     KQKNVAKTFGVRSTPFMIFFTKDGEEISRIPGYIPADEFLVKLNSVLDKE"
     gene            complement(1717..2073)
                     /locus_tag="VKL21_01815"
     CDS             complement(1717..2073)
                     /locus_tag="VKL21_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44536.1"
                     /translation="MKRNTVLVIGILATIMLFGAAVALAQNSSENKKDTENHECTPEM
                     MNDMSKNCPGQMMKSGEGKNMMDGKKGHNDSGMMGNGKPAGDNTDPASGSHCGDMNPG
                     TGSMMGSRGSDLKRMM"
     gene            complement(2079..2273)
                     /locus_tag="VKL21_01820"
     CDS             complement(2079..2273)
                     /locus_tag="VKL21_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44537.1"
                     /translation="MDCCGKTKVTDKDIVKEQTGTEKNPVENEHNHGGGCCGGGTKDM
                     LLHLVLMIVAFLIISYFLKR"
BASE COUNT          807 a          521 c          415 g          907 t
ORIGIN      
        1 tatcattaag cctgagcata ccatcgtata tttcattacc tttgtcaaat atctttaaaa
       61 ataaaatcgg aatttgtttt gtgtccttgg atccagttat ttctgattta agagggacgg
      121 tcatatatac catcaacacc ccttcaagtc ccatatcccc aatttcaaaa gacgcaacat
      181 atcttccact actatcggaa tcagtcgctg ttatataaga accggaatat acggtccctt
      241 cctgattttt caagatcaat aaaggagcca gaccatagac attactcata aacgtgtatc
      301 ctttataact catcagatta tttgaataga ctatatctgt tttaacttcc ttcccgttct
      361 cttttatgac aagctttcct gcattatctc tgagcgttcc ggtttcatca ttgtaatcgg
      421 aatagaactt ctcaagattg atatcaaatt tctgatgatt atcataaaaa aatggtccct
      481 catttacaaa aacataattc ttatgatctt ccgaaagtgt ctgcccttcg attaatcgca
      541 tatctccctc catcctaccc aaacttccat acactgcagc aaatattata ataagtatac
      601 agacatgaaa tagaaatgta ccgaaaactc cgtatctgtt tttttcagcg aaaatgcaat
      661 tttcttcctg gaatattcta tatccaccgg atttaagtac agaatttatc acagaaatga
      721 tatatttttg ttctttttcc gttctaagtt ctatattatt atcgagccca ctgatatatg
      781 gcttttgttg gatttgcgga tatcttagcc ttcgataagc ggtattgaac attatccgag
      841 tacaaaaaaa ggtattgatg aaaagaaggg ccgccaggcc tataaatatt ggagatgaaa
      901 aaagacctgt caggccaagt atctcgaaat aatatgcctc ggtgggatgg ttggttttcc
      961 agacattata tacatctgac ttgagctgtg atttttgggg tatgtgtgtc ccgattattg
     1021 agaatacgat aataagcaaa ataagaacca ttgccaattt tcgggatcta aaaaaaactg
     1081 caattttttt ccatgttaag aaatccattg ttttatcatt ccttatccag aaccgaattt
     1141 aatttaacta ggaactcatc tgctggaatg tatccgggaa ttcttgatat ctcttcacca
     1201 tctttagtga agaaaatcat aaatggagta cttcgcacac caaatgtttt tgcaacattt
     1261 ttctgtttga atgtatctat cgaaacgata acaaaattat tattaagtat gtcgataact
     1321 cttttatccg ataatgcctc ttcttcgaat tttttgcacc agtaacaggt ttcggatctg
     1381 aaataaagaa aaatcggttt gctaagattc tttgcctcat taattcctgt atctattgaa
     1441 gtatggaatg acaaaccgcc cagtttaata aatgcagcat cgccttctga ttgtgagttt
     1501 tcccaccagc ttgctgccaa gaacaggata gaacctactg caataaatga aatcagtttg
     1561 tttatcggct tcatttcagt ttaatccata aaaattaata attatatttt tctattaaaa
     1621 catgtggaat gaaagttata acaaaaactt aagatataag aaaactgcat ccaccagttt
     1681 tcttatatgc cttcatttgt ggtaaaataa aagatattac atcattcttt tcaggtctga
     1741 acctcttgaa cccatcattg aacccgtgcc aggattcata tctccacaat gactcccgct
     1801 tgctgggtca gtattatcac ctgcaggctt tccattaccc atcattccgc tatcattatg
     1861 gcctttttta ccatccatca tatttttgcc ttcgccagat ttcatcatct gccccgggca
     1921 attttttgac atatcattca tcatttcagg agtacattca tgattttctg tatccttttt
     1981 attctcactt gaattctggg caagcgcaac agcggcgccg aataacataa ttgtcgccag
     2041 aattccaatg actaaaacag tatttctttt catatttttc acctctttaa aaagtacgaa
     2101 attattaaga atgcaactat cataagtaca aggtgcagca acatatcttt ggttccgccg
     2161 ccacagcaac caccgccatg gttgtgttca ttttctacag gatttttttc agtgcctgtt
     2221 tgttccttta ctatatcttt atccgttact ttcgtttttc cacagcagtc catttttaat
     2281 tctccgtatt aattttttag tatcttgtat aaatgtatcg tctatattta tggtcatttt
     2341 gctttaaatt ttgaagatat tctcaacatt aattttttta tgcacaaact atataattta
     2401 caatgcttgt aaagtaatta atttacattg gcattgggct ttgctggaga tataaaagcg
     2461 acaattacaa gtgagaataa gaaatatgac tgaagatgac atagaagcca ttgaaaagga
     2521 gatgatagag gaactgaaga ggccgcctgg caggaggata cttgtctgtc ctgtctgcgg
     2581 ctgcactgat gtcacatatt atatgggcct taaaatagga tatcagtacc agtgcaagag
     2641 ctgcaattat
//
LOCUS       DATGBN010000082         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_44834, whole genome shotgun sequence.
ACCESSION   DATGBN010000082 DATGBN010000000
VERSION     DATGBN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44834"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VKL21_01825"
     CDS             complement(<1..187)
                     /locus_tag="VKL21_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HMB44538.1"
                     /translation="MSVEERLNKIVEKEGAVHLTLLDPDSQSPELAGKIAREAYLGGT
                     DAIMVGGSTGATGTTVEH"
     gene            complement(192..341)
                     /locus_tag="VKL21_01830"
     CDS             complement(192..341)
                     /locus_tag="VKL21_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HMB44539.1"
                     /translation="MARFPEAEKVLLHMKICMKCNARNSVRATRCRKCGYKNLRMKSK
                     ELKGT"
     gene            complement(424..630)
                     /locus_tag="VKL21_01835"
     CDS             complement(424..630)
                     /locus_tag="VKL21_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HMB44540.1"
                     /translation="MQKNRVIHTIPTEGIYKGIPVVVTPVHDGEGHPIAALGIVDVVC
                     TIDLASVFGNYPQIVRQVEEGKKR"
     gene            complement(612..1226)
                     /locus_tag="VKL21_01840"
     CDS             complement(612..1226)
                     /locus_tag="VKL21_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HMB44541.1"
                     /translation="MLTKSEGEMAVRLARTAIKECLNNERKIEPENLPDIFKEKRGVF
                     VTLNKKTSSKELRGCIGRPYPVLPLGEAIILSAINAAREDPRFHPVELQELDELVIEV
                     TVLTVPKRINAKPGDIPDKIMIGRDGLIVSTGMSSGLLLPQVAVEHGFDSAEFLCQTC
                     MKAGLMPDAWLEGAQVYSFEGQIFEEVEPCGKIREKDIKCKKIV"
     gene            complement(1293..1862)
                     /locus_tag="VKL21_01845"
     CDS             complement(1293..1862)
                     /locus_tag="VKL21_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44542.1"
                     /translation="MSEFYNILTKALANKQMSISSLARELKKKGYDQHRLILTGYLRA
                     LHEMGRLEEIDIPPSKVYILRSGMNRDVYSIVKEHLKDIDISERLEIAVFILNSLFHR
                     PCFKFELELLGIEARKTETVRESKDARLKEHRTAVTRIKIPIDDPAFEMNGDSRDIQL
                     RGTKVMIDIINDLIDLDGLKAKYQQTKLA"
BASE COUNT          538 a          491 c          399 g          688 t
ORIGIN      
        1 tgtgttcaac agttgtcccg gtagccccgg tggaaccgcc gaccataatt gcatctgttc
       61 ctccaagata cgcttctctg gctattttac cagccagttc gggggattgc gagtccggat
      121 ctaaaagggt aaggtgaaca gcaccttctt tttcaactat cttgttgagc ctttcctcaa
      181 ctgacatcaa attacgttcc cttaagttcc tttgatttca ttctaagatt cttatatccg
      241 cattttctgc atcgtgttgc acgcactgaa ttccttgcat tacatttcat gcagattttc
      301 atgtgtaata gtactttttc cgcttctgga aatcttgcca tagttcacct ctggtggact
      361 gcctacttga atgttatgac atttaaccct tttgtatcaa aattctatac tttatttggt
      421 tcactaacgt ttcttacctt cctcaacctg ccgcacaatc tgcgggtaat tcccgaatac
      481 tgaagcaagg tcaatagtgc agacaacatc cacaataccc agagctgcta tgggatgacc
      541 ctccccgtca tggacagggg taacaacaac tggtattcct ttatatatcc cttcagttgg
      601 tatcgtatgg atcacacgat ttttttgcac ttaatatctt tttcccttat ctttccgcat
      661 ggttctactt cttcgaatat ctgtccttca aaggaatata cctgcgcccc ttcaagccat
      721 gcatcaggca taagtccggc tttcatgcat gtctggcaca ggaattcggc gctatcaaat
      781 ccatgttcca cggcgacctg gggaagcaga agtcccgaac tcatgcctgt tgaaacaata
      841 agaccatccc ttccgatcat tattttatcg ggtatatctc ctggtttggc attgatgcgt
      901 ttcgggacag taagtacggt cacctcaata acaagttcat caagttcttg cagctcaacg
      961 ggatgaaatc gcgggtcttc gcgcgcggca ttgatagccg ataatatgat ggcttctccc
     1021 aggggcagta ccggatatgg tctgccgata cagccccgaa gctcctttga gcttgttttc
     1081 ttatttaatg ttacaaaaac gccgcgtttt tccttaaaaa tatcgggaag attttccggt
     1141 tcgatctttc tttcattatt aaggcactct ttaatagctg ttcttgccag gcgtacagcc
     1201 atttctccct ctgattttgt aagcatgatc aaggattatc ctgcgggaat atatgttttt
     1261 ttgaattcct tcaataactc tacaatccag acttatgcaa gttttgtctg ctggtatttt
     1321 gctttcaatc catcaaggtc gatcagatca ttaataatat caatcatgac tttggtccca
     1381 cgaagttgaa tatctctgga gtccccgttc atctcaaatg ccggatcatc gatgggaatt
     1441 ttaatgcgtg tcacagccgt cctgtgctcc ttgaggcgtg catcctttga ttcccttact
     1501 gtttctgtct tgcgtgcttc gatccccaat aattcaagtt caaatttgaa gcatgggcga
     1561 tggaaaagag agttaaggat aaatactgca atttctaacc tttcagagat gtcaatatct
     1621 ttcaaatgtt ctttcacaat actataaaca tcacggttca tcccggatct taaaatatat
     1681 acttttgaag gcggtatgtc gatctcttcc aggcgaccca tttcatgtaa agcccgaaga
     1741 tatcctgtaa gtatcaacct gtgctggtca tatcctttct tttttaattc tcttgctaaa
     1801 ctgcttatgg acatctgttt atttgccagt gcttttgtga ggatattata aaattccgac
     1861 atgaacaaat tagatttggt aacaaacctg ataaatgttt gggtgttgaa catcatcata
     1921 gaaaaataaa gggattcttg aaagacaggt tcaaatgttc caggtaaaaa caataattat
     1981 atgcatttgc cgcatacatt gaaaccatgt aacctacccc gcacttaagt gcggggcatt
     2041 ataactatgt tcagcatcaa ttcccttgcg ggcttcagca tcatcacacg ctatgtatgg
     2101 cgaggttaca gttcgc
//
LOCUS       DATGBN010000083         7996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_45002, whole genome shotgun sequence.
ACCESSION   DATGBN010000083 DATGBN010000000
VERSION     DATGBN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45002"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VKL21_01850"
     CDS             complement(<1..433)
                     /locus_tag="VKL21_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44543.1"
                     /translation="MHIDPINDDILNFTAHCIEKAKLCVCMGDTLTHLDSFEDVRNLF
                     ADVYRSLEPGGKFLLTFRDLIFELKDNERFIPVKSDSNTIFTCILEYETEHVRVNDMI
                     YTKDHDMWKLDVSSYRKLRIPQEWVRKELRIDEFGWCISSSE"
     gene            complement(456..692)
                     /locus_tag="VKL21_01855"
     CDS             complement(456..692)
                     /locus_tag="VKL21_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44544.1"
                     /translation="MATVKEHYEKHIAQYYSWLFGDFNERVESNRHFFIENKLLPEKS
                     ETAIDLGAGSGFASVALAQIGYNVLAIDMPDAAG"
     gene            833..1540
                     /gene="tatC"
                     /locus_tag="VKL21_01860"
     CDS             833..1540
                     /gene="tatC"
                     /locus_tag="VKL21_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMB44545.1"
                     /translation="MNSVPGDKELPLGEHILELRSRMLVVAIPVILITAIVFIYSGEL
                     LKMIWNNAIPVPMTIYSPMELMMTRFKLSLATALFIGIPLLVYEAFIFIGKGLYPNEK
                     KFFIKVVPFSFLLFAFGAFLAYFLVLPIIFNYTIFYSIDVADPQVSVIRTIDTIITLI
                     LGFGLVFQFPLLLISAIKMGILKTGFIKGKRKFVYGALIAFAFFITPDPTAISELIVA
                     FVLLILFESSLIIARYF"
     gene            1597..2856
                     /gene="hisD"
                     /locus_tag="VKL21_01865"
     CDS             1597..2856
                     /gene="hisD"
                     /locus_tag="VKL21_01865"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HMB44546.1"
                     /translation="MILKSISGLTNIEIENLLKRTGELHEVMPAVTSILNDIKDNGDP
                     ALLKYTAQFDKATFEEIEVSHKEIHEAVYSLDKNILIHLKKAASNIRSFHEAQVPKDW
                     IKEFSPGIRLGQRISPLGSVGVYVPGGRATYPSTALMTIIPAKVAGVRQVIMCTPPNP
                     DGKVNPLTLAAAHIAGAGRVFRVGGVQAIAAMAYGTKTIPKVDKIVGPGNVYVTAAKM
                     ACGTAIDFPAGPSEVLIIADESARADFIASDMIAQAEHDPNAVSVLVTTSSDLPGLVK
                     QEIDIQIKNAARGEIINKSLENAAILTGTLDECIDFSNKFAPEHLEIMTREDILGRIK
                     HAGSIFIGPYAPVSAGDYASGTNHVLPTAGYAGMFSGLNIDHFITKSSVQIIEKNGLK
                     EISETIIGLAEAEGLFAHANAVKVRKV"
     gene            2909..4834
                     /gene="uvrB"
                     /locus_tag="VKL21_01870"
     CDS             2909..4834
                     /gene="uvrB"
                     /locus_tag="VKL21_01870"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMB44547.1"
                     /translation="MSFKLTSVFEPKGDQVKAIEKLTSGLQQGMKHQTLLGVTGSGKT
                     FTIANVIQKIKRPTLVISHNKTLAAQLYSEFKAFFPENAVEYFVSYYDYYQPEAYIPQ
                     TDTYIEKDASINEEINRMRLSATRSLFERRDVIVVASVSCIYGIGSPQEWHDMSILLW
                     KGDESPRKKILSRLVDMQYERNDLDFSTGKFRARGDTIEVFPSYASTGVRIELFGDEI
                     ERISEFDPLTGKTLLEKDALVIYPAKHFVMPQEKIDRAIESIEDELKERLQELKRTGK
                     ILEAARLEQRTKFDIEMMHEIGYCQGIENYSRHMDGRKAGEPAYTLIDYFPRDFLIVI
                     DESHVTIPQIRGMHAGDHARKESLVDYGFRMPSAYDNRPLTFDEFSERINQVLYVSAT
                     PADYEIARSAQVVEQIIRPTGLVDPEIVVKPITGQIDDLIGEIHAKTVKKSRVLVTTL
                     TKRMAEDLTEYLLGVGIRARYMHSEIETLERIEIIRSLRLGEFDVLVGINLLREGLDL
                     PEVALVAILDADKEGFLRSDRSLLQTIGRTSRNIEGRVVMYADRMTGSMQRAIEETTR
                     RREKQIEYNKEHNITPQTIRKAVEPRAITQEAPPKEEIFNYIVEMEAQMHRAAKNQEF
                     EKAAKIRDRIAVLRKEM"
     gene            complement(4922..5506)
                     /locus_tag="VKL21_01875"
     CDS             complement(4922..5506)
                     /locus_tag="VKL21_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HMB44548.1"
                     /translation="MQKNIEYLKSSWKYIYIMSGIFIISVIAGLLISLKDLGLPENYL
                     EIFKNSFGWIKTLSPLSIMLVIFLNNAVKSLFAIVLGAGFGIIPIIFVGGNGIILGLI
                     ANGVSKQQGIIFVLAALVPHGIIEIPMILISAGLGLRLGYFMYLSLRGEKKDMRSELS
                     GSLRLYMRLIMPLLFVSAMIETFITPVILMWVSG"
     gene            complement(5508..6368)
                     /locus_tag="VKL21_01880"
     CDS             complement(5508..6368)
                     /locus_tag="VKL21_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HMB44549.1"
                     /translation="MSIIDSVWHYIDKYYLSGIINDTSYNPVDTLTYAIVLGISLFGV
                     LKLLEKLNVKIDAQFMISVTPYIIAGSSLRVLEDLKVFTPPLQYLFVTPIIYFFMFAV
                     TMSLLTIAIDLERKGKIRDYHTFFGYTGAAWAFLNLCALLVFGSVENLYPAIVILILG
                     IVSTGIVYFISLQLNFALLTDRVNISILFTHLLDASSTFIGMDWLNYYEKHVAPTFFI
                     DLAGNFTDHPSLVMFPLKLLVFIPVLYMLDNKLEDEKERKLIGIMKLAILVLGLSPAV
                     RNILRILMGV"
     gene            6380..7447
                     /locus_tag="VKL21_01885"
     CDS             6380..7447
                     /locus_tag="VKL21_01885"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HMB44550.1"
                     /translation="MKETNQNYKNKWMQLPRNVVVGHGVINETGKVCKDLKLNGSALI
                     VTGATTLKMAGKTVALSLEESGFNVNTTIVEKPTIDRVKKVESLASEAKASFLLGVGG
                     GKSIDIAKLASMHLDIPFISVPTAASHDGIVSSRASIIRDNRTASESAHTPLGVVADT
                     AIIAAAPYRLLAAGCGDIISNYTAVRDWELAHRLRDEPFSEYASIISKLTAKILIDSA
                     ELIKPGLEESAWTVMKALVASGVAMSIAGSSRPASGSEHKFSHALDEIAPNPALHGEQ
                     CGVGTIMMMYLHGGNWQEISASLKTIGAPTNASELGIGEEYIIKALLHAHKIRPERYT
                     ILGTGLTREAAEKVAKITKVI"
     gene            7493..7942
                     /locus_tag="VKL21_01890"
     CDS             7493..7942
                     /locus_tag="VKL21_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HMB44551.1"
                     /translation="MEESDTIITLIGTRLAKVGNEFIFKSAAKECQPCKFNKTCLGLN
                     PGSRYKIVNLRNSAKLECFVHDSGVLAVEVIEAPIRIAVESRKAINGSKIVYEPPPCN
                     NQDCENYPICRPLGLRTGDKLSIVEVIGEISEPCRKGFSMKIVEVKR"
BASE COUNT         2449 a         1645 c         1798 g         2104 t
ORIGIN      
        1 gctcgcttga tgaaatacac cagccaaatt catctatccg taattccttt cttacccatt
       61 cctgcgggat tcgaagcttc ctgtagctgc taacatccag tttccacata tcatggtctt
      121 ttgtatagat catgtcgttt actcttacat gttcagtctc atattcaagg atgcaggtaa
      181 atatagtatt tgagtcgctt ttgaccggaa taaatctttc attgtctttt aactcgaaaa
      241 tcaagtccct gaaagtaagc aagaatttcc ctcccggttc aagagaacgg taaacatctg
      301 caaacaggtt ccggacatcc tcaaaagaat caaggtgagt caatgtatcg cccatacata
      361 cacacaactt tgctttttca atacaatgag ctgtaaagtt caatatatca tcatttattg
      421 gatcaatgtg caaatttcca ctatggtttt ttaattcatc cagcagcgtc tggcatatca
      481 atagcaagga cattataacc tatttgtgcc agggctactg acgcaaatcc agaccctgcc
      541 cccaggtcaa ttgcagtttc ggatttttcc ggtaacagct tattttcaat aaaaaaatgg
      601 cggttggatt caactctttc attgaaatcc ccgaacagcc atgaataata ctgtgctata
      661 tgcttttcat aatgttcttt tacagttgcc atatgaattc cttcttgaat cgatagatac
      721 tttcttccat gtttggaaag ataacacctg ggtataggca agaaaaagtc gttcccggat
      781 aaatatattc cgccttcctc tactttatat gaataaagaa taattaacaa taatgaattc
      841 tgttccaggc gataaggaat tgccccttgg ggaacatatt ttggaactgc ggtcaagaat
      901 gctggtagtg gcgataccgg tcattctaat tacagctatc gttttcattt attcaggaga
      961 actcttgaaa atgatctgga ataatgcgat ccctgtgccc atgacaatat actccccgat
     1021 ggaactgatg atgacacgat ttaagctctc tttagccacc gcgcttttta taggcattcc
     1081 actccttgtt tatgaagcat tcatattcat agggaaagga ctttatccaa atgaaaaaaa
     1141 gtttttcatc aaagtcgtcc cattttcatt cttattattc gcattcgggg cgttcctggc
     1201 ttattttttg gtgttgccca ttatatttaa ttacacgata ttttattcaa tagatgtagc
     1261 agatccgcag gtttctgtaa taagaacgat cgacaccata attacattga tacttggttt
     1321 tggacttgtg tttcagtttc ctttactcct gatatctgcg atcaagatgg gtattttgaa
     1381 aacagggttc attaagggaa aacgcaaatt tgtttatggg gcgcttattg catttgcatt
     1441 tttcataact ccagatccca ccgcaatttc agaattgata gtggcgttcg ttcttctgat
     1501 attgtttgaa tccagtctga taatagccag atatttttga tccacaatat tatttagaca
     1561 aacctttatc tgcctgcgcc ctgaaatcag tgtgtaatga tactgaaatc catttccgga
     1621 cttacaaata ttgagataga aaacctgcta aagcgcacag gtgagttgca tgaggtaatg
     1681 cctgccgtaa catccatact caatgatata aaagataatg gtgatccagc cctattaaaa
     1741 tacacagcgc agtttgataa agcaacattt gaggaaatcg aagtttccca caaggaaata
     1801 catgaggctg tgtattccct tgataaaaat atcctgatac atttgaaaaa ggcagcttcc
     1861 aacatccgtt cattccatga ggcccaggtc ccaaaggact ggataaaaga gttttccccg
     1921 ggcatcaggc tgggacagcg tatttcacct ctagggtcag tgggtgtcta tgttccgggc
     1981 gggagggcga cttatcccag tacagccctt atgacgatca taccagcaaa agttgcaggc
     2041 gtcaggcagg ttatcatgtg cactccaccg aatcctgacg gtaaagtaaa cccattgaca
     2101 ctggcggcgg cccatatcgc aggcgctggc cgtgtttttc gcgtgggagg agtacaggcg
     2161 atagctgcta tggcttatgg gacaaagacc attccaaaag tggataagat cgtaggccct
     2221 ggaaatgtat atgtcacagc cgccaaaatg gcatgtggaa cagcgattga ttttccggcc
     2281 gggccaagtg aagtgctgat aatcgcggat gaatcggcaa gagcggattt tatcgcatcc
     2341 gatatgatag cccaggcaga gcacgatccg aatgccgtct cagtgcttgt aaccacatca
     2401 tcagaccttc cgggacttgt aaagcaagag atagatatcc agatcaaaaa tgcggcgcgg
     2461 ggagaaataa taaataaatc tcttgagaat gcagccattc tcacaggaac acttgatgaa
     2521 tgtattgatt tttcaaataa atttgctccg gaacaccttg aaattatgac tcgtgaggat
     2581 attcttggaa ggataaagca tgcaggttcg atatttatcg gtccttatgc tcctgtatct
     2641 gcgggggatt atgcttcggg caccaaccat gtgcttccaa cagccggata tgcgggaatg
     2701 ttctccggtt taaatataga ccatttcatt acaaaatcaa gcgttcagat aatagaaaag
     2761 aatgggctaa aggaaataag tgaaacaatt attgggcttg ccgaagccga agggcttttc
     2821 gcccatgcaa atgctgtgaa ggtacggaag gtctgaagta aggattcctt ttatttcaat
     2881 tgatgaatta taaactgaag ggatagaact gtcatttaaa cttacatcgg tattcgagcc
     2941 aaaaggcgac caggtaaaag cgatagagaa actgacttcc ggtctccaac agggtatgaa
     3001 acaccagacc ctgctggggg tcacaggttc cgggaaaacc ttcacgatcg ccaacgttat
     3061 tcaaaaaatc aaacgcccga cgcttgtaat ttctcacaac aagacccttg cagcacagct
     3121 ttactccgaa ttcaaggcat tttttcccga aaatgctgtt gaatattttg tgagctatta
     3181 cgactactat cagcctgaag cctatatccc gcagactgat acctatatag aaaaagacgc
     3241 ttccataaac gaggagataa acaggatgcg gctctctgcc acaaggtcgc ttttcgagcg
     3301 ccgggacgtc atcgtggtgg cgagcgtttc ctgcatttac ggcatcggat caccgcagga
     3361 atggcatgat atgtccattc ttttgtggaa aggggatgaa tcgcctcgaa aaaaaatact
     3421 ttcaaggctg gtggatatgc agtatgagcg aaatgatctt gattttagca caggaaaatt
     3481 ccgggcgcgc ggtgatacga tcgaagtatt tccttcctat gcatcaacgg gggtaaggat
     3541 cgaactcttc ggcgatgaga ttgaaagaat ttcggaattt gatcccctga cagggaaaac
     3601 gctccttgaa aaagatgccc tggtcatcta tccggcgaag catttcgtta tgcctcagga
     3661 gaagatcgac cgggctatag aatcaataga agatgaacta aaagaaagat tacaggaact
     3721 aaaacgaacg ggaaaaatac tggaagcagc ccgtctggag cagcgcacaa aatttgatat
     3781 cgagatgatg catgagatcg ggtactgcca ggggatcgaa aactacagcc gccacatgga
     3841 cgggcgaaaa gccggtgaac cggcatatac tttgatagat tattttccca gggattttct
     3901 tatcgttatc gatgaatcgc atgttaccat tccgcagatc cgcggcatgc atgctggaga
     3961 ccatgcaaga aaggaatctc ttgttgatta tgggttcagg atgccatctg catacgataa
     4021 ccgtccgctc accttcgatg aattcagtga acggataaac caggttttat atgtttctgc
     4081 gaccccggca gattatgaga tagccaggag cgcacaggtc gttgaacaga taatccgccc
     4141 cacagggctt gttgaccctg agatcgtagt aaaacccata accggacaga tcgatgacct
     4201 gatcggtgag atccatgcaa aaacagtgaa aaaatccagg gtccttgtaa caacgcttac
     4261 aaaacgaatg gctgaagacc tgacggaata tcttcttggt gtggggatca gggcaaggta
     4321 tatgcattct gagatcgaga cgcttgagcg catagagata ataaggagct tacgtcttgg
     4381 tgaattcgat gtgcttgtgg ggatcaatct cctgcgtgaa ggtcttgacc tgccggaagt
     4441 ggcgcttgtt gcaatacttg acgctgacaa ggaaggtttc ctgcgctctg accgctcgct
     4501 tttgcagacg ataggaagga catcaaggaa tatcgaggga agagttgtga tgtatgcgga
     4561 caggatgaca ggttcgatgc aaagggcgat agaggagaca accaggcgcc gggagaagca
     4621 gatagaatat aataaggagc ataacatcac gccgcagacc atacgaaagg ctgttgagcc
     4681 gcgtgcgata acgcaagagg cgccgcctaa agaagagatt ttcaattata ttgtggagat
     4741 ggaggcccag atgcaccggg ctgcgaagaa ccaggagttt gagaaggctg caaagataag
     4801 ggacaggata gcggtgttga ggaaggagat gtgagtttgc acgggctttc atataaaacc
     4861 agcattcaag gtaatatatt agacacaaca aaatcgttca cgatcccgat aatgtaatat
     4921 cttaacccga aacccacatt agaattaccg gcgtaataaa ggtctcgatc attgcagata
     4981 cgaaaaggag aggcataatc agcctcatat ataaccgcaa actcccggac aattcagacc
     5041 tcatatcttt cttttcaccc ctcagtgaaa gatacatgaa atagccgagg cgaagcccaa
     5101 ggcctgcgct gatcaatatc atgggaattt ctataatgcc gtgcggtacg agtgcggcga
     5161 gtacaaaaat gatgccctgc tgctttgaaa ccccattcgc gatcaaaccc aatattatcc
     5221 cgttccctcc cacaaaaatg atgggaatga tgccaaatcc tgcgccaagc acgatcgcaa
     5281 acaggctttt gactgcattg ttcaggaaaa tgaccagcat tatcgataga ggggaaagag
     5341 tctttatcca tccaaaagaa ttcttaaaaa tctcaaggta attttccggc aatccgagat
     5401 ccttcaaaga tataagcaat ccagcgatta ccgaaatgat gaaaatgcca gacattatat
     5461 aaatatattt ccatgaagat ttaagatatt caatgttttt ttgcatttta tactcccatc
     5521 aggatcctta gtatgtttcg aacagcaggt gaaagtccga gcactaatat cgcaagtttc
     5581 ataataccaa taagttttct ttctttttca tcttctaatt tgttatcaag catatataat
     5641 actggtatga acaccaacaa tttaagtgga aacatgacca gggatggatg gtcagtaaaa
     5701 tttcctgcaa ggtcgataaa aaatgtggga gctacatgct tttcataata attgagccaa
     5761 tccattccga taaatgtgga agaagcatcc agaagatggg taaaaagtat ggatatattt
     5821 accctgtccg tgagaagcgc aaaattgagt tgaagggata tgaaataaac aattcctgtg
     5881 gagactatac ctaagatcag tataacaatt gcaggatata aattctcgac gctgccaaac
     5941 accagcagcg cgcagagatt caagaatgcc catgctgctc cggtatatcc aaaaaacgta
     6001 tggtagtccc tgatctttcc ttttcgctca aggtcaattg cgatcgtaag taggctcatc
     6061 gtaaccgcaa acatgaaaaa atagattatg ggtgtcacaa agagatattg taagggggga
     6121 gtgaatactt ttagatcttc cagcacgcga agagatgaac cggcaatgat atacggggtg
     6181 acggaaatca tgaactgcgc atcaatcttc acgttgagtt tttccaataa ctttaagact
     6241 ccaaaaaggc ttataccaag cacgattgca taagtgagcg tatccacagg attataagat
     6301 gtgtcgttga ttatgccact taagtaatac ttatctatat aatgccagac gctatctatt
     6361 atactcatat tgtaacctta tgaaagaaac taaccaaaac tataagaaca aatggatgca
     6421 gctgcccagg aacgttgtcg taggtcatgg tgtgattaac gaaacaggta aggtctgtaa
     6481 agaccttaag cttaatggga gtgcgttgat agtaaccgga gccaccacgc ttaagatggc
     6541 gggaaaaacg gtagcgctct cccttgaaga gtccggtttt aatgtaaata caacaatagt
     6601 tgaaaaaccc acgatcgaca gggttaaaaa agtagaaagt cttgccagcg aggcaaaagc
     6661 ttcgttcctg cttggagttg gtggaggaaa atccattgac atagccaagc tcgcttctat
     6721 gcatcttgat atccctttta taagtgtccc tacggcagca tctcatgatg gtattgtctc
     6781 atcaagagcg tcaattataa gggataatcg gactgcatcc gaatcggcgc acactcctct
     6841 tggagtagtt gctgatacag ccattattgc agcagcgcct taccgcttgc ttgcggcagg
     6901 atgcggagac atcatatcca attatactgc ggtgcgagat tgggaactcg cccacaggct
     6961 tcgcgatgaa cctttcagcg aatatgcatc cataatatca aagctcactg caaaaattct
     7021 catcgactcg gccgaactga taaaaccagg tcttgaagag tctgcatgga cagtgatgaa
     7081 agctcttgtt gcaagcggcg tagccatgag tatagcaggt tcttcgcgtc ctgcaagcgg
     7141 ttctgagcat aaattcagcc atgcgcttga tgaaatagcc cccaatccgg ctctgcacgg
     7201 tgagcaatgt ggagtaggga ctattatgat gatgtacctt catggcggga actggcagga
     7261 gatcagtgca tccttgaaga cgatcggagc acccacaaat gcttccgaac tcgggatagg
     7321 ggaagagtat attataaaag cactccttca tgctcataag attcgccctg agagatacac
     7381 tatacttggt acgggattga cgcgtgaagc agcagaaaaa gttgcgaaaa ttacaaaagt
     7441 aatatagcta taatataatc ataactatta atataaaatt aggtgaaatt atatggaaga
     7501 atctgataca ataataacgt tgataggaac aaggctggca aaagtaggga atgaatttat
     7561 ttttaaaagc gctgcaaaag aatgccagcc gtgtaaattc aataaaactt gcctgggtct
     7621 taatcccgga agcaggtata aaatagtgaa tcttcgaaac tctgcaaagc ttgagtgttt
     7681 tgtgcatgat tccggtgtgt tggcagtaga agtcatagaa gcccctatca ggatagctgt
     7741 ggaatcaagg aaagcaataa atggttcaaa aatagtttat gaaccgccac catgtaacaa
     7801 ccaggattgt gaaaattatc ccatttgccg gcctttgggt cttcgtacgg gcgacaaatt
     7861 aagcatagtg gaagtaatag gtgagatttc cgaaccctgc aggaaaggtt tttcaatgaa
     7921 gatcgttgag gttaaaagat aacaaatgcg gacttcgaag gagcctatag aaagcaaagg
     7981 ctttgtttgg caaatt
//
LOCUS       DATGBN010000084         5547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_200859, whole genome shotgun sequence.
ACCESSION   DATGBN010000084 DATGBN010000000
VERSION     DATGBN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200859"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VKL21_01895"
     CDS             <1..605
                     /locus_tag="VKL21_01895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMB44552.1"
                     /translation="SYERSLTEGVSILYMASMFYGYPLYVYFNFSGYCDVVIAMGRWA
                     GFKIPENFNKPYISRDMIEMWNRWHITLSQLVRDYLFQPLLKFLISGPLSGYVRTSQY
                     ISIFITFFLVGIWHGTTINFVVFGLLQGLGMAVSMIYRDQGIRQLGKIKYKMYYDNKW
                     VANIERFFCLHFWCFTFLFFEYDIFKMTGWLFKLLGGAYG"
     gene            751..1833
                     /locus_tag="VKL21_01900"
     CDS             751..1833
                     /locus_tag="VKL21_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44553.1"
                     /translation="MKRNYKLVLVIFFAVSIVYAAFLVMSENYIMFREEIQYIKFNEG
                     DSFYTPYKNDLPWEINVIYNEINSLSSENNLILIGASTTREGIIPEQLATPAKWKLKN
                     MAIGADTIYSFRLMRNYLNSYANHKPDKTDIVVIHIFYGSFSITSPKNDYLRQTIEML
                     GTYQVDDSAQVHGYMSGMRKLWELSNYKIRFALGLNEGSVGKSLVYAVRDFGTKSIRL
                     IIGDSNEIHNSSPTAEDLAKYRTFWTTYTQNVTFPGKSTDDFKELLKELKSQTNVVIV
                     NLYVPSWHRTYPSEQEYENWTRADLIPFLNEQGISWIDLSRSIPDSEYGDSVHLFKGG
                     RENYTKQFSTAISPILSNFDRLQKFN"
     gene            1888..2886
                     /locus_tag="VKL21_01905"
     CDS             1888..2886
                     /locus_tag="VKL21_01905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB44554.1"
                     /translation="MREEEIRPKAIFDKYLELAREDTVTFFSNKDDFDYISCPACEID
                     NADVQFEKEGFKYVLCRNCGTLYANPRPGFEIIQQYYKNSKSTRFWAEYFYKDTEKQR
                     REKIFKPRAIKISEFIKNNFISKPEVLVDIGAGYGAFLVEMKKLNAVNKIIAIEPSDD
                     LAGICREKGFQVIQSPLEKVGGILRTKVSVATSFELFEHLHNPRQFLSRVKEILSENG
                     YFILTTLNIEGFDLQVLWEKSNSIAPPHHLNFFNLYSIEYLLQHSGFEVVMKTTPGFL
                     DVDIVKNAYKDGSIKADRFIEHLIKKSDTKTQEKFQTFLRENRLSSHMCIIARKEI"
     gene            2883..3812
                     /locus_tag="VKL21_01910"
     CDS             2883..3812
                     /locus_tag="VKL21_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HMB44555.1"
                     /translation="MKILISSASFGKINKDPLTILEKAGFEPLLNPYGRKFEPGEFAG
                     LIKDAVGLIAGTEKITAELLENAPMLKVISRYGVGLDNIDLEAAKKRGIIVRSTPEAP
                     SQAVAELALILILNLNRRVSEADRNTRQKNWSQLMGRLLSDKSLGIIGLGRIGKKLIK
                     LVQPFNMKIFAYDPFPDIDFISSNNLTLKSLNEILSQSDIISLHLPLLDKTFHVIGKK
                     ELSLMKRDAIIINTSRGGLIDEPALVEALKGGIIGGAGIDTFEDEPYKGNLIEFENVI
                     LTCHMGSSTIETRKQMELETVNNLIGALKEARS"
     gene            3809..4717
                     /locus_tag="VKL21_01915"
     CDS             3809..4717
                     /locus_tag="VKL21_01915"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMB44556.1"
                     /translation="MKAIVFGGSGFLGSHVADFLTKSGYKVTIYDIVKSPYLQKGQNM
                     AVGDILDADAVRKAVKGCDVVYNFAGIADLEKAKEKPLKTVEINILGNTTILDACREH
                     SVKRFVFASSLYVYSKTGSFYRSSKQACELIIENYFEEFGVPYTILRYGSLYGPRADE
                     NNWVYRTLTQAITKGKITRYGDGEELREYIHVEDAARCSVDILSYEYKNEYVIITGYQ
                     QMKIKDMMTMINEMLGNRIELEFLPAKSNLHYEITPYSFSPKIAKKYVSKYYLDIGQG
                     VLQCLEDVYKKHHKYKSVNGLLLHED"
     gene            4734..5363
                     /locus_tag="VKL21_01920"
     CDS             4734..5363
                     /locus_tag="VKL21_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HMB44557.1"
                     /translation="MICAIIFDFDGVIIESFDIKTQAFRELFKIYRQVDEIVEYHKQN
                     GGVSRYIKFRHIYSEILKQPLEEKTFIELGEKFSNLVVEEVKKCPYVPGALEFIREKS
                     KQMKLFIASGTPEDELVAIVNARGISEYFNGIYGSPAIKSEIIQKILIKENFNNKNVV
                     FIGDTITDYREASKAGVHFIARINGSFDNPLLELAVPKIHDFYEYCIKL"
     gene            5341..>5547
                     /locus_tag="VKL21_01925"
     CDS             5341..>5547
                     /locus_tag="VKL21_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMB44558.1"
                     /translation="MNIASSYKILSYPINEISPMYGSAPPPTFKPYSQIVKGDTSNSL
                     IISIHNHTGTHIDAPKHFIDNGKSI"
BASE COUNT         1899 a          890 c         1137 g         1621 t
ORIGIN      
        1 cgtcctacga acgctcactg actgagggtg tttctatatt atatatggct tctatgttct
       61 atggttatcc gctttatgtt tatttcaatt tcagtggtta ttgtgacgtg gttatagcca
      121 tgggcagatg ggcaggattt aaaatcccgg aaaactttaa caaaccttat atttcaagag
      181 atatgataga aatgtggaac agatggcata taacgctttc acaattggta agagattact
      241 tgttccaacc tttgttaaaa tttcttatct ccggtccgct ttctggatat gtccgaacga
      301 gtcagtacat aagtatattc atcactttct tccttgtagg aatctggcat ggtactacta
      361 taaattttgt tgttttcggg ttattacagg ggctgggcat ggcagtttca atgatttaca
      421 gggatcaggg tataaggcaa cttggcaaaa taaaatataa gatgtactat gataataaat
      481 gggttgcaaa tattgaacga ttcttctgtt tgcacttctg gtgctttaca tttttattct
      541 ttgaatatga tatttttaag atgaccggat ggctctttaa gcttttggga ggcgcatatg
      601 gataaaacaa gagaaaaatc agggatgtta tggtctatat tatggttatc ggtgaagatt
      661 gtgttaatta tagttctgat tactggcgca aacagagtat tagtactcta tcagaatttc
      721 taaaatctaa aataatgcta aaggtgctcg atgaaacgaa attataagct ggtattggtc
      781 atattttttg cggtgagtat agtttatgct gccttccttg taatgtctga gaattatatt
      841 atgttcaggg aggaaatcca atatattaag ttcaatgaag gggatagctt ttacacgcct
      901 tataaaaacg atctcccgtg ggaaataaac gtaatatata atgaaataaa ctcattatca
      961 tccgaaaaca acctcatact tattggcgct tctacgaccc gtgaaggaat tattccagaa
     1021 caattagcaa ctcctgcaaa atggaaattg aaaaacatgg cgataggagc agatacaata
     1081 tacagcttca ggctgatgcg aaactatctg aatagctatg ctaatcataa accggataaa
     1141 acagatatcg tggttatcca catattttat ggaagttttt ccataacatc cccgaaaaac
     1201 gattatttgc gacaaacgat cgaaatgtta ggaacgtatc aggttgatga ttcagcgcag
     1261 gtgcatggat atatgtccgg tatgcgtaaa ctctgggagc ttagtaatta caaaatacgc
     1321 tttgcactgg gacttaatga gggttcggta ggtaaatctt tggtttatgc agtacgggat
     1381 tttggaacaa aaagtatacg tttgataatt ggcgattcca atgaaattca caattcaagt
     1441 ccaactgcag aagaccttgc gaaatacaga actttctgga ctacatatac tcaaaatgta
     1501 acttttccag gcaaatcaac agatgatttc aaagagttat tgaaagaact taaatctcag
     1561 acaaacgtgg taattgtaaa tctttatgtg ccctcttggc acagaactta tccatcagaa
     1621 caggaatatg agaactggac tagggccgat ctgattcctt ttctaaatga acaaggaatt
     1681 tcttggatag atctttctag atccattcct gactcagaat atggagattc agtgcatcta
     1741 tttaagggtg gaagagaaaa ttatactaag caattcagta cggcaattag tccgatttta
     1801 tctaattttg atcgactgca aaagtttaat taagaaagga ttttattaag taaaataaag
     1861 ttttagcaaa taataaagag ataaataatg agagaagaag agattagacc aaaagcgatc
     1921 tttgacaaat atttagaact tgcaagagag gatacagtaa cctttttttc aaacaaggat
     1981 gattttgact atatttcctg tccggcctgc gaaattgata atgccgatgt tcaattcgaa
     2041 aaggagggat tcaaatacgt tttatgcagg aactgcggta cactatatgc taatcctaga
     2101 cctgggttcg aaattatcca gcagtattat aaaaattcca aatcaaccag attttgggca
     2161 gaatattttt ataaagatac cgagaagcaa cgaagagaaa agatttttaa gcccagggca
     2221 ataaaaatat ctgaattcat aaaaaataat ttcattagca aaccagaagt tttagtcgat
     2281 atcggggccg gatacggtgc atttttagta gaaatgaaaa aattaaatgc tgtaaataaa
     2341 attattgcaa tcgaaccttc agacgacctt gccggaatat gcagggaaaa aggttttcaa
     2401 gtaatccagt cccctcttga gaaagtgggg ggcattctga gaactaaggt atctgtcgcg
     2461 acgtcgtttg agcttttcga acacctgcat aatccgaggc aattcctgag cagggtcaag
     2521 gaaatccttt cagaaaatgg ttattttatt cttactacct tgaacatcga gggttttgat
     2581 cttcaggtgc tatgggaaaa atcaaacagc atcgctccgc cccaccacct taattttttt
     2641 aatttatatt ccatagaata tttgctccag cacagcggtt ttgaagttgt aatgaaaaca
     2701 acccccggat tcctggatgt agatattgtg aaaaatgctt ataaagacgg atcaataaaa
     2761 gcggacaggt tcatagagca tttaataaaa aaatccgaca ctaaaacaca ggaaaaattc
     2821 cagacattct tgagagagaa caggttaagt tcacatatgt gcataatagc acggaaggaa
     2881 atatgaaaat attaatttcc agtgcttcat tcgggaagat caataaagat cccttgacga
     2941 tccttgaaaa agccggattt gagcctttgc ttaatcccta tggcaggaag tttgaacccg
     3001 gtgagttcgc cgggctaatc aaggatgcag tgggcctgat tgccggaacc gagaagataa
     3061 cagcagaact gctagagaat gcaccgatgc taaaggtcat atccaggtat ggtgtgggat
     3121 tggacaatat tgatcttgaa gctgcaaaga aacgggggat catagtcaga agtacccctg
     3181 aagcgccttc gcaggccgta gcagagcttg cccttattct aatactcaat ttgaatagga
     3241 gagtaagcga agcagataga aataccaggc aaaagaactg gtcgcaattg atgggcagac
     3301 tgctttcaga taaatcttta gggataatag gactgggaag aattggcaaa aaattaataa
     3361 aacttgtcca gcctttcaat atgaaaattt ttgcctatga tccttttccg gatattgact
     3421 ttatttcatc aaataattta actctcaaat cattaaatga aatattatct caaagtgata
     3481 taatatcttt gcacttacct ttattagata aaacgtttca tgtgatcgga aaaaaagaat
     3541 tatcattaat gaagagggat gctatcataa tcaatacgtc tcgcggaggc ttgattgatg
     3601 aacctgctct cgttgaagct ctaaaaggcg gcataattgg cggtgcaggt atcgacactt
     3661 ttgaagatga accatataaa ggaaatttga tagagtttga gaatgttatt ttgacctgtc
     3721 atatgggctc atcgacaata gaaacgcgta aacaaatgga attggaaact gtaaataacc
     3781 tgataggcgc attgaaagag gccaggtcat gaaggcaata gttttcgggg gttcgggttt
     3841 tcttggaagt catgttgcag atttcctgac aaaatcagga tataaggtaa caatatatga
     3901 tatagtaaaa tctccatatc tccaaaaggg gcagaatatg gcagtgggag atatccttga
     3961 tgctgatgct gtaagaaaag cagtaaaagg atgtgatgta gtttataatt ttgccggaat
     4021 tgctgatctt gaaaaagcaa aagaaaagcc cttaaaaacc gttgaaataa atattctggg
     4081 aaatacaaca attcttgatg cctgcagaga acatagtgtt aaaaggttcg tattcgcaag
     4141 cagtctttat gtttatagta agacaggatc attctataga agcagtaaac aagcatgtga
     4201 attaataata gagaactatt ttgaagaatt tggcgtgcca tataccattc taagatatgg
     4261 ttccttatac ggtcctagag cagatgagaa caattgggtt tacaggacat tgacccaggc
     4321 gatcacaaag ggtaagatta cacgctacgg tgatggcgaa gagttgaggg aatatataca
     4381 tgttgaagat gctgcaagat gcagtgttga tattctttct tatgaatata agaatgaata
     4441 tgtgattatc acgggttatc agcagatgaa aatcaaagat atgatgacca tgatcaatga
     4501 gatgcttggc aatcgtatag aactggagtt tctgcctgct aaatcaaatc tccattatga
     4561 aataacccca tattcattca gcccgaaaat agcaaaaaaa tatgtcagca agtattatct
     4621 tgatataggc cagggagtat tacaatgcct tgaagacgta tataaaaaac atcataagta
     4681 taaaagtgtc aacggtctat tattgcatga ggattaaaaa aaactgataa aatatgattt
     4741 gtgcaataat ttttgatttt gatggtgtta ttatcgaatc gtttgatata aaaacacagg
     4801 cattcaggga gctttttaag atatataggc aagtagatga gattgtcgaa tatcataaac
     4861 agaatggtgg agtatcgagg tatataaaat tcaggcatat ttacagtgaa atactgaagc
     4921 agcctcttga agaaaagact ttcatcgagc ttggagaaaa attttcaaat ttagttgtag
     4981 aagaagtaaa aaaatgccct tacgttccag gggcgcttga atttatccgg gaaaaatcaa
     5041 aacaaatgaa gttgtttatt gcatcaggga cacctgaaga tgaattggtg gcaatagtga
     5101 atgcccgcgg catttctgaa tattttaatg gcatttatgg cagtcctgcc ataaaatccg
     5161 aaataataca aaaaattctc attaaagaga atttcaacaa caagaatgtc gttttcatag
     5221 gagatactat tacggattac agggaagcca gcaaggctgg agtgcatttt atagcccgga
     5281 ttaatggttc attcgataat ccactattag aattagccgt gccaaaaatc catgattttt
     5341 atgaatattg catcaagtta taaaatccta tcttatccta taaatgagat cagtcctatg
     5401 tatggaagtg ctcctcctcc aacattcaaa ccttatagtc agatagtgaa aggggataca
     5461 agtaacagtc ttataatttc catacacaat cataccggaa cacatataga tgccccaaaa
     5521 cattttatcg ataacggaaa atcaata
//
LOCUS       DATGBN010000085         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_278496, whole genome shotgun sequence.
ACCESSION   DATGBN010000085 DATGBN010000000
VERSION     DATGBN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278496"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VKL21_01930"
     CDS             <1..383
                     /locus_tag="VKL21_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44559.1"
                     /translation="LKNHSEIGWVLREYNTLAESANKLGIKSPEIYYHEGKLNGKKSK
                     DKSNDVKSSGKAADPVKMVPVPEIKIESGDMIEEFEVLRSQRKPGCNELPNLVKKAKS
                     IEMPQLLKGFKPLRLSHSKLKKQS"
     gene            481..1143
                     /locus_tag="VKL21_01935"
     CDS             481..1143
                     /locus_tag="VKL21_01935"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMB44560.1"
                     /translation="MNIILLGPPGAGKGTQAKKIQEHYSLPHISTGDILRENINNNTS
                     LGMKAKSYMSRGELVPDELLITLIKDRLSKNDCNAGFMLDGYPRTIPQADALQMILTE
                     SGRKVDAILNISVNDEELIKRLSGRRMCKCGASFHIMLNRPDKEDICNACKGKLYQRD
                     DDKPEAIRNRLNVYKKQTQPLIDYYDRKKILRTINGSKEISQIFEDIRKVLEEYDQKD
                     TK"
     gene            1121..1714
                     /locus_tag="VKL21_01940"
     CDS             1121..1714
                     /locus_tag="VKL21_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HMB44561.1"
                     /translation="MIKKTLNKLVMVLGIFMMFGIIMFPNMREVIGKLMNTLLEPILG
                     GLPIHMIIFVLAAFTGIYASLIQKYTMDWGLMKRVQEKSRTIQKEMRQAQLTNNAQKI
                     KKLEAQYAEMMGDQMNMFKQQFKPMLYISIISIPLFYWVYLLIGQNPDATMIFPFWGV
                     QKLNATVWIFQYWLFWYFLCSIPISQITRKSLNIGGM"
     gene            1715..2236
                     /locus_tag="VKL21_01945"
     CDS             1715..2236
                     /locus_tag="VKL21_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB44562.1"
                     /translation="MKITISGPPGSGKSTLSKIISVKFGLELVSIGDIFRKCAQDRCM
                     SLDEFGQIARCNEKIDHQIDEMQKTIANKKDNILIEGRLSGFIVEADLKVWLKAPLEV
                     RAQRIANRECKSLEKAMQETSVREECERERYLKHYEIDIKDLSVYDIVIDSSKWSAQE
                     ISGIVEKAVDFLI"
BASE COUNT          791 a          409 c          493 g          574 t
ORIGIN      
        1 gactcaaaaa ccactctgaa attggctggg tattaaggga atataatact ctcgctgaga
       61 gtgccaataa gctaggaata aaatccccgg aaatctatta tcacgaaggc aaattaaatg
      121 gtaagaaaag caaagacaag agcaatgatg taaaatcttc cggaaaagct gccgatcctg
      181 tgaaaatggt ccctgttccg gaaataaaaa ttgaatccgg tgatatgatc gaagaatttg
      241 aagttctgcg gtcacaaaga aagcccgggt gcaatgagct tccgaatctt gtaaaaaagg
      301 caaaatccat cgagatgccc cagcttctta aaggttttaa acctcttcga ttatctcatt
      361 caaagcttaa aaagcagtct tgaaaagatt ttcgcggtac atccttatac aattttttac
      421 ttttagcgcc gattttgaaa agctttaata tccacataac cattacggga ttcgatatta
      481 atgaatatta ttttactagg gcctcccggc gcaggaaagg gcacacaggc caaaaaaata
      541 caggaacact actcacttcc ccatatttcg accggggata tcctgcgtga aaatataaat
      601 aataatacaa gtcttggaat gaaggcaaaa tcatatatgt caagaggcga gcttgtcccg
      661 gatgaacttt tgataactct catcaaggac agattatcta aaaacgattg caacgcagga
      721 ttcatgttag atggctatcc aagaacgatc ccacaggcgg atgcactgca gatgatattg
      781 acggaatcgg gaaggaaagt ggatgccatc cttaatatct cggtaaatga tgaggaactc
      841 ataaagcgcc tatcggggag gagaatgtgt aaatgtggcg caagttttca tattatgtta
      901 aatcgtcctg acaaagaaga tatatgtaat gcatgcaaag gaaaattata tcagcgcgac
      961 gatgataaac ctgaagccat aaggaacagg cttaatgtat ataaaaaaca aacgcagcca
     1021 ttgatagatt attacgatag gaaaaaaatt ctcaggacaa tcaatggaag caaggaaatc
     1081 tcacagatat ttgaagatat aagaaaggta ttggaggaat atgatcaaaa agacactaaa
     1141 taagcttgta atggtccttg gtatttttat gatgtttggc attataatgt ttcccaacat
     1201 gagggaagta ataggcaaac tgatgaacac acttttagaa ccgatattgg gggggcttcc
     1261 gatacacatg ataattttcg ttctggcggc ttttacgggt atttacgcat ctctcatcca
     1321 gaaatataca atggactggg gattaatgaa acgcgtccag gagaaatcca gaacaattca
     1381 aaaagaaatg cgccaggccc aattaacaaa taatgcccag aaaataaaaa aattagaagc
     1441 gcaatatgca gaaatgatgg gggaccagat gaatatgttc aagcagcaat tcaaacccat
     1501 gctttacata agtattattt cgatcccgct tttctactgg gtatatcttc tcataggcca
     1561 gaaccctgat gccaccatga tttttccgtt ctggggagta cagaaattaa atgcaactgt
     1621 ttggatattc caatactggc ttttctggta tttcctgtgt tcgattccca tcagccagat
     1681 aaccaggaag tcattgaata taggcggaat gtaattgaaa atcacgatca gcggtcctcc
     1741 cggaagcggc aagagcacac tatcgaagat aatttcagtg aagtttggcc ttgaactggt
     1801 atccataggg gatatcttcc ggaaatgtgc tcaagaccgc tgcatgtctc ttgatgagtt
     1861 cggccagatc gccagatgca atgaaaaaat agatcaccag atcgatgaaa tgcaaaaaac
     1921 gatcgcaaat aaaaaggaca atatcctcat cgaaggaagg ctttcaggtt ttattgtaga
     1981 agctgacctt aaggtctggc ttaaagcgcc tcttgaagta agagcccagc gcatcgcgaa
     2041 tagggaatgt aaatccctgg aaaaagcaat gcaagagaca tctgtaaggg aagagtgcga
     2101 gagggagcgc tatttgaaac attatgaaat agatatcaag gatctatcgg tttatgatat
     2161 tgtgatagat tcaagcaagt ggagcgcaca agagataagt ggaatagtgg aaaaagcggt
     2221 tgatttcctg atatgatatt accttctgaa aaaaaaagag agacatt
//
LOCUS       DATGBN010000086         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_123635, whole genome shotgun sequence.
ACCESSION   DATGBN010000086 DATGBN010000000
VERSION     DATGBN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123635"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..966)
                     /locus_tag="VKL21_01950"
     CDS             complement(31..966)
                     /locus_tag="VKL21_01950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB44563.1"
                     /translation="MISVVVPFYNEEENIEPLYSELSDTLKKMNREYIIIFIDDGSTD
                     NTFKNMLKVREKDNKLKIIKFRKNFGQTAALKAGFDLARGDIVISMDGDLQNDPADIP
                     KLIEKIEKEDYDVVCGWRADRKDTISKKFTSKFANLLRKMTTGEVIHDSGCTFRAYKI
                     GCVKGLDLYGEIHRYIPAMLLWRGYKIGEEKVKHHSRKFGKTKYGLLRIGKGFLDLIV
                     ISFWQKYSVRPIHVFGGLGLLLALSGTLIGGYMGFERLFLGKSLSDRPLFLLAVLMVI
                     IGVQFIVSGILADIMLKVYYGQNERKNYLTEKVIE"
     gene            1308..2132
                     /locus_tag="VKL21_01955"
     CDS             1308..2132
                     /locus_tag="VKL21_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HMB44564.1"
                     /translation="MLIWTILTLIFVLNPLLSETVIRVVLGIPMLLFMPGYVLMAVLL
                     PKTNELEGIERIALSFGLSIAAVPLLGLFLNFTFNTRLLTILLTLCIFTLIFIFIAAF
                     RLGRVPEDERFSVPFDKIQKVIMEEFNASKGKSDKILAWILILSIVLAIGMMIFVILT
                     PKIGEKFTEFYILGPNGKAENYPSQLKYGSNVSVLVGVVNHEYTRVNYTVKVVLDTEI
                     LNETGFYLDHNNTWENYISFAPDKEGIEMEIEFWLFKDDDFSSPYRDLHMWVNVTQ"
     gene            2129..>2379
                     /locus_tag="VKL21_01960"
     CDS             2129..>2379
                     /locus_tag="VKL21_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44565.1"
                     /translation="MKKEIYLPMLGIAAGEIMMLSGHEYIGLVLHVINLQAVTLALIF
                     SDLSMEKKNLIQTLLLLLQMRILNLAMPQFFTTKLLWYPL"
BASE COUNT          701 a          482 c          420 g          776 t
ORIGIN      
        1 ggggatattc ataattaagc atcagtattc tcattcgata accttttcag ttaaatagtt
       61 ctttctctca ttctgcccat aataaacctt gagcataata tccgcgagaa tccctgatac
      121 aataaattgt acgccaatga tcaccatcaa aacagccagt aaaaacagag gcctgtcaga
      181 aagactcttc cccaggaaca gcctctcaaa acccatatat ccgcctatga gcgtgcctga
      241 tagggccagt agcagcccta atcctccaaa aacatggatt ggtctgactg agtatttctg
      301 ccagaagctt atgactataa ggtcaaggaa accttttcct attctaagca aaccatactt
      361 ggttttaccg aatttcctgg agtggtgttt aactttttcc tcgccgatct tgtagcccct
      421 ccacagcagc attgcgggga tgtacctatg gatttccccg tacagatcca gaccctttac
      481 gcatccgatc ttgtatgccc tgaaagtgca tcctgaatcg tgtatcacct cacctgtggt
      541 cattttcctc agtaaatttg cgaatttaga tgtaaatttt ttggagatgg tgtcttttct
      601 atccgctcgc catccgcaaa ctacatcata atcttccttc tcgatctttt cgatcagctt
      661 cggaatatcc gccgggtcgt tttgcagatc gccatccatg ctgatgacaa tatccccccg
      721 ggctagatca aatccagcct tgagggctgc ggtttgtccg aagtttttcc tgaactttat
      781 tattttgagc ttattatcct tctcacgaac tttcaacatg ttcttgaagg tattatctgt
      841 ggaaccatca tctatgaaaa tgataatata ttcccggttc atttttttca atgtatcgct
      901 taattcagaa tacagaggtt ctatattttc ttcctcattg taaaaaggaa ctactacgga
      961 tatcatttag cttcaccttt atgccaattc aaaaattttt ccatgaaaac caggttcgaa
     1021 atcgtataga ttcaacatca taaaatacct ataataacat tcgttctaca atacgtatta
     1081 gtatatatat ttactcaagc acataaataa atattatgat tctaattatc atattgattt
     1141 tttcaggaag attcaagctg gattatcaag accagacaaa aaaattatgt tatgcaacag
     1201 tccttcctaa aaaataatta tatatatttg tatatcagtt taacctaaga ttttactggt
     1261 atatttgaat gaaaaaacaa tataaatttc ctttagactt attaattatg ctaatatgga
     1321 caattctcac gcttatcttt gtccttaacc ctttactaag tgagactgtt atcagggtcg
     1381 ttttgggcat accaatgctc cttttcatgc caggctatgt cctgatggca gtattgttac
     1441 caaaaacgaa tgaacttgaa ggaatagaac gtattgcatt gagtttcggc ctgagtatag
     1501 ctgcagtacc ccttcttggt ctattcttga attttacttt caatacaaga cttttaacga
     1561 tattactgac actttgtatt tttacgttaa tatttatttt catcgcggca ttcaggctgg
     1621 gaagagtccc tgaagatgag aggttctctg taccgttcga taagattcaa aaagttatta
     1681 tggaagaatt caatgcgtcg aaaggcaaat ccgacaagat cctggcatgg atacttattt
     1741 tgtcaatcgt tttagccatt ggaatgatga tttttgtaat attaacacca aaaataggtg
     1801 aaaaatttac ggaattttat atcctgggcc cgaatgggaa agctgaaaat tatccttcac
     1861 agttaaaata tgggtctaat gttagtgttc ttgtgggggt ggtaaatcat gaatataccc
     1921 gggtgaacta tacggttaaa gtggtacttg ataccgaaat tttaaatgag actggtttct
     1981 acctggacca caataacaca tgggaaaatt acatatcctt tgcgcctgac aaagaaggca
     2041 ttgaaatgga gattgaattc tggcttttca aagatgatga tttttcttcg ccttatcgtg
     2101 atctgcacat gtgggtgaat gttacgcaat gaaaaaagaa atctatcttc caatgctggg
     2161 aatagctgca ggggaaatta tgatgttatc gggccatgaa tatattggtc ttgtattgca
     2221 tgtaatcaac cttcaggcag tcacgcttgc cctgatattc agcgatcttt ccatggagaa
     2281 gaaaaacctg atacaaaccc tgttattgtt actccagatg cggatattga accttgctat
     2341 gccccaattc tttaccacca aattgctctg gtatcccct
//
LOCUS       DATGBN010000087         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_201196, whole genome shotgun sequence.
ACCESSION   DATGBN010000087 DATGBN010000000
VERSION     DATGBN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201196"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1454)
                     /locus_tag="VKL21_01965"
     CDS             complement(255..1454)
                     /locus_tag="VKL21_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB44566.1"
                     /translation="MGNWDLIKGYTGCADADIEPRIAMQIVNEAALCSNRIRRSNYIS
                     HQGFELLNGLGFLVTDKQVHNLLDKHTVIEAQSLQETLAVIRSNNGHYKGNLIAIDPH
                     RIVSTSQRIMPKKKKQPEEPARKMLQTFFALDTQTGQPIGCGIGSPGANTTEATIDLL
                     NMVNMVSKNALILADKEHFTEYLIRSIDQNSDFEFLVPAISTERIQKIERSQTYQRKW
                     AGYAIAETMFNFDGHKEKYRLISQREGEITRNYVYKSFLTLSGKPATVLLSEMYQERW
                     SIEDFFNFDGAMGFDRASTFNLNIRYGKMSLALLAQAATYQFRQKLPKPYNRWNSTHL
                     ADAVFAKIDGDIRVKDDTIIITCYNAPKELNLHNHYQNLPDKLISEGINPKVPWLYDY
                     KLDFRFK"
     gene            complement(1628..2029)
                     /locus_tag="VKL21_01970"
     CDS             complement(1628..2029)
                     /locus_tag="VKL21_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HMB44567.1"
                     /translation="MEILINGIKGKDFVIDKKDSLCKKLAMLIEGHCTIGVQSALQKY
                     GYTEQRYYQLLRKYEQGGSQALQDKKPGSDKQPVRTRETLNQIIRLRFLDPFASADII
                     AQKLNQIGHKVSKRSVERTITEYGLQKKHMF"
BASE COUNT          680 a          502 c          448 g          827 t
ORIGIN      
        1 attgtcatcg gtgtgagaat ttgtcatggt ttttgccaat cccggtaagt gcgcaggaaa
       61 tgtcatgtca tgataaaatt ttctagaagc gaatagtccc ggtaagtggg caggatttcc
      121 gtgtcacgat aaagatgtcc agatgactga gtcccgatag atgggcagga attgtcccgt
      181 catgataaaa ctgtccaagt ggctcccgat agctatcagc gatttttcag ggggtattca
      241 ttttagtaaa aatcttattt aaacctgaaa tccaatttat aatcataaag ccagggaacc
      301 tttgggttaa ttccctcaga tatcaacttg tccggcaaat tctggtaatg attatgcaaa
      361 tttagttctt ttggcgcatt ataacacgtt ataataattg tatcatcttt taccctgata
      421 tctccatcta tttttgcgaa caccgcatct gccaaatgag ttgagttcca ccgattatac
      481 ggtttgggaa gtttttgtct aaactgatac gttgccgcct gtgccagtaa agccaaagac
      541 attttgccat agcgtatatt caaattaaat gttgaagctc gatcaaaccc catagcaccg
      601 tcaaaattaa agaagtcttc tattgaccac ctctcctgat acatttcact tagcagtact
      661 gttgctggct taccggatag ggttaagaat gatttgtaca cataatttct tgttatttct
      721 ccttcacgct gggagatcag cctgtatttt tctttgtgtc cgtcaaaatt aaacatcgtt
      781 tccgctattg cataccctgc ccattttctt tggtaggttt gagatctttc tattttctgt
      841 attctttctg ttgatattgc agggacaagg aattcaaaat cgctattctg atcaatactt
      901 cgaattaaat attccgtgaa gtgttccttg tcggccagta ttagagcgtt cttgcttacc
      961 atgtttacca tattcagcag atctattgtt gcctcggtag tattagcccc aggcgaacct
     1021 ataccacatc ctatcggttg cccggtttga gtatccaagg caaaaaaggt ctgtaacatt
     1081 ttccttgcag gctcttccgg ttgctttttc tttttcggca ttattctttg agaggtggat
     1141 acaatacgat gagggtctat ggctattaag ttccctttat aatgaccatt attcgacctt
     1201 atgacagcta atgtttcctg caatgattga gcttcaatga ccgtgtgctt atcaagcaaa
     1261 ttatgtacct gcttatcggt taccagaaac ccaagtccgt ttagtaactc aaagccctga
     1321 tgggaaatat aattactcct tcttatacgg ttactacaca gagcagcctc attaactatc
     1381 tgcatagcta tacgtggctc aatatctgca tcggcacatc cggtgtaccc tttgatcaaa
     1441 tcccaattgc ccaagcgcaa gtgttcagca gccagaaacc ataaaccgac taacgtcccg
     1501 catattttct tcgagagact ttccctgaat aactgctcca ccctgtggtt attgactttg
     1561 acctttattg tctcccgctt tgttttatgt atttcaatat cttgtttttg tttctccttt
     1621 ttggggttca gaacatgtgt tttttttgga gtccatactc cgtaattgtc ctttccacac
     1681 tacgtttgct caccttatgg ccaatctgat ttaacttttg agcgataata tccgcacttg
     1741 caaaggggtc taaaaaccgg agtcgtatga tttggtttaa tgtttctctg gtcctgacag
     1801 gctgcttatc cgagccaggc ttcttatcct gtagggcttg tgaccctcct tgctcgtatt
     1861 ttctcaacag ttggtagtac ctttgttcgg tgtacccata cttttgtaat gccgattgca
     1921 caccaatggt acaatgccct tcaattagca tggccagttt cttacacaaa ctatcttttt
     1981 tgtcaataac aaaatctttt cccttgatgc cgttaattaa aatttccatc gtttcaacgt
     2041 ttagtttaac aactaatata ctaaattaat atatggcggt gaagatataa ataattttaa
     2101 ataacaaaaa gaaaaccagg atattaattc acttgaatac aaatttatat gtaatattga
     2161 aaaatggttt aaacaataat tatgaaatac aaccgccata tataaattta gcttttacaa
     2221 tagttttgca aagtagcttt aggacgataa ataatgaatt gcacggttac tttgtaaata
     2281 gatgggcatg aaaaaatccg actgaacttc agcatatgga actaaagtaa atagcacctg
     2341 aaaaatcgct gatagctatc gggacggtaa tcagtggtct ttgacgatag aactacttaa
     2401 tttattaaaa agtgttcggt tatcggtaat caataatcag tggtgagtcc catagac
//
LOCUS       DATGBN010000088         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_395425, whole genome shotgun sequence.
ACCESSION   DATGBN010000088 DATGBN010000000
VERSION     DATGBN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395425"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VKL21_01975"
     CDS             complement(<1..641)
                     /locus_tag="VKL21_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB44568.1"
                     /translation="MVGFINRKNEIRILEDIYSSNSSSLVVIYGHRRVGKTELSREFI
                     KGKKAVYFFIEIKHESMIFKDMEESLGEILKIKPRIDSWDDFFKLIFEQKEKLIVVFD
                     EFQNLGKVNSGIFSKFQKYWDLNHNKSQHMFLIIGSYVGMIKKIFKDTKEPLFGRATM
                     LFNIKPFNFFDSFTFLKAFFDLEIEDASKFYFIFGGVPKYLLLAGQMGTDDPV"
     gene            complement(675..1703)
                     /locus_tag="VKL21_01980"
     CDS             complement(675..1703)
                     /locus_tag="VKL21_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HMB44569.1"
                     /translation="MNKGDIVKQQSDFILYTGKDGKVNIDVFVQDENVWLTQKAMGLL
                     FGVESHTITYHLKEIFKSGELDEKATTRKIRVVQNEGERQVTRKLDFYNLDAIISVGY
                     RVNSYQATQFRIWATRILKEYIIKGFVLDDERLKQGNQVFGKDYFDELLERIREIRAS
                     ERRFYQKITDIYALSADYDKNAPITMEFFANVQNKMHWAITGKTAAEIIYDSADASKI
                     HMGLTNWKQGLDGKIMKSDVSIAKNYLDETHIKELNQIISAYLDLAENRAQRQILMKM
                     ADWIEFLHNFLGLSSYPILKDKGKVSALEAKLKAEREYEVYRFQQDKDYISDFDKEIK
                     RITGKKAP"
     gene            complement(1696..>1977)
                     /locus_tag="VKL21_01985"
     CDS             complement(1696..>1977)
                     /locus_tag="VKL21_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HMB44570.1"
                     /translation="YEYFLLKTLNFEEMNSDSAVPGLNRDIALSTEIKIAPLDKLNDF
                     NQYCSTLLDRLNANNQQIRTLTRLRDTLLPKLMSGEVRVKLKEEEAVYE"
BASE COUNT          543 a          418 c          340 g          676 t
ORIGIN      
        1 tttaccgggt catcagttcc catctggcca gcaagaagga gatatttggg aacgccacca
       61 aagataaaat aaaattttga tgcatcctcg atttctaaat caaaaaaggc cttcaggaaa
      121 gtaaaactat caaaaaagtt gaatggtttt atattaaaaa gcatcgtagc tcttccaaat
      181 agcggttctt ttgtatcttt gaatattttc tttatcattc caacataaga acctattatc
      241 aggaacatat gctgcgattt attatgattt aagtcccagt atttctggaa ttttgaaaat
      301 ataccggaat tgactttacc cagattctgg aattcatcaa acaccacgat caatttctct
      361 ttctgctcaa agatcagttt gaagaaatca tcccaactat caatccgcgg ttttattttg
      421 agtatctctc caagcgactc ttccatgtct ttaaaaatca tggactcatg ttttatttct
      481 ataaaaaaat agacggcttt ctttcctttt atgaattcgc ggctcaattc ggtttttccg
      541 accctgcggt ggccatagat caccacaagg gaagatgaat ttgagctgta aatatcctcc
      601 aatatgcgaa tttcattctt tcggttgatg aagcctacca tatagtaaca tactgttcag
      661 tttgtatata agaattatgg tgcttttttt cctgtgatgc gctttatctc tttgtcaaaa
      721 tcagagatat agtctttatc ctgttgaaaa cggtacacct catattcccg ttcagccttg
      781 agtttagcct ccaatgcgct gactttgccc ttatccttca agatcggata gcttgacaat
      841 cccaaaaagt tgtgtagaaa ttcgatccag tccgccattt tcatcaaaat ctgcctttgc
      901 gcacgatttt ccgccaaatc aagataagcc gaaataattt gattgagttc tttaatatgc
      961 gtttcgtcca ggtaattttt tgcaatggac acatctgact tcattatttt cccgtccagc
     1021 ccctgcttcc agtttgtcaa acccatgtgg attttgcttg catcagctga atcataaata
     1081 atttcagcag ccgttttgcc ggttatagcc caatgcatct tgttttgtac attggcgaaa
     1141 aactccattg ttattggggc gtttttatca taatctgccg agagggcata tatatcagta
     1201 atcttttggt aaaagcgacg ctcacttgcc cggatttccc gtatgcgctc caacagctcg
     1261 tcaaaatagt ctttaccaaa aacctgattg ccctgcttga gtctttcatc atccaacaca
     1321 aatcctttga tgatgtattc tttcaggatt ctggtagccc agatgcgaaa ttgggtggcc
     1381 tggtaggagt tgacgcggta gcctactgag atgatggcat cgaggttgta gaaatcaagt
     1441 tttcttgtta cctgcctttc accttcattt tgaactactc gaatttttcg agtggttgct
     1501 ttttcatcaa gttcacctga cttgaaaatc tcttttaaat gatatgtaat tgtatgactt
     1561 tcaacgccga aaagtaagcc catagctttt tgtgtcagcc agacgttttc atcctgcaca
     1621 aaaacatcaa tatttacttt tccgtctttt cctgtgtaga gtatgaaatc tgattgttgt
     1681 tttacaatat ctcctttatt catatacagc ctcttcttcc tttaatttca ccctcacttc
     1741 cccgctcatc agcttcggca gcagcgtatc ccgcaggcgg gtgagagtgc ggatttgttg
     1801 attatttgcg ttcagtctat ctaataaagt agaacaatat tggttaaaat cattcaattt
     1861 atctaaaggc gcaattttta tttccgtaga aagtgcaata tctctattaa gaccgggaac
     1921 cgctgaatcc gaattcattt cttcaaaatt aagtgtcttt aataagaagt attcata
//
LOCUS       DATGBN010000089         1950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_240134, whole genome shotgun sequence.
ACCESSION   DATGBN010000089 DATGBN010000000
VERSION     DATGBN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240134"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VKL21_01990"
     CDS             <1..422
                     /locus_tag="VKL21_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HMB44571.1"
                     /translation="IDYQVLFYPVTDDNFDTQSYQEFATDIWLTRGAMKWFWDNYLPD
                     ENARKQPTASPLQASIDQLKGQPPALIITDENDVLRDEGEAYAHKLIQAGVNVTSVRY
                     LGTIHDFLMLNALAGTPATCSAIGLANENLRNVFAKS"
     gene            complement(675..1772)
                     /locus_tag="VKL21_01995"
                     /pseudo
     CDS             complement(675..1772)
                     /locus_tag="VKL21_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          510 a          415 c          356 g          669 t
ORIGIN      
        1 taattgacta tcaagtgctt ttttatcctg tgactgatga taattttgat acccagtcat
       61 accaggagtt tgcaaccgat atctggctta cccgtggagc catgaagtgg ttctgggata
      121 attatttgcc cgacgaaaac gcgcgcaagc agccgacggc atccccttta caagcttcaa
      181 tcgaccagct caagggacag ccaccagctc ttatcatcac tgatgaaaat gacgtcctcc
      241 gtgacgaagg tgaggcgtat gctcataagc tgattcaagc cggtgtcaat gttacatctg
      301 ttcggtacct gggaactata cacgatttct tgatgctaaa tgcacttgca ggcacacctg
      361 caacttgcag cgcaatcggc ctggcgaatg aaaatcttag gaacgttttt gccaaatcgt
      421 aattgaatgg ctgctccctc gctgtaagct ggctacttag gacccattac gaaataacct
      481 aggtaacgca taattattga ggattaaatc aacgggcctg cttgtgaaaa tggatgagta
      541 cagtctaaaa agatgcctat gttatttcgc ggtggatcct tagcaggtaa aagggaaaac
      601 gcttggggat aaatacttca attaaggatt ataggtctag ggatattaat ggtgctagtg
      661 tctcgaccat ttaattattg agcataatac ttggacattt tttcatccgc gttctttctt
      721 gaaaatttcc attctattct cttttcaagt tcattccttt tcttctccca agcacctact
      781 tcatgggtaa gtatctcaat atcaccgatt cttctgtctg tacattcaat atccatcaca
      841 ttaatctcta tttctgcggc attgagccaa cttgcatgtt ttggcgtgta atggaattcg
      901 atcttgtcta gaatcttttt tgcttcgtct tcgctgaacg cctcataaaa tgacttttcg
      961 ttgtggatgt tgagattatc agtaatcaac ctgatgaatt cagcatcaga atatttctcg
     1021 gtaatgagaa ttttcatgaa ttctgcaaaa tctatccttg ttctcctttt agttacctga
     1081 gtcacccttt tccctgcttt gaactctaca gccacgaata tatttgctgt cccgttcctg
     1141 atatactcat aatcatactt ttctgaactc ccaggtttta tgggaattga ttttctttta
     1201 tccctaagaa gttgttttgg tttctcgtcg agacagacaa taggtttttt tggatcgaaa
     1261 tcttcttcgt acaggtcaag aacgtcgtac attctttttc tgaattcgga atcaatcgtc
     1321 tgaatgcacc acatcttttt cagtcagggt tttgttttgt tttctttcaa aatgagtctg
     1381 atgctctctt tacctatagt ttcaaatcct tcttttttcc tcaattcctc acaaagaagt
     1441 gtaagtgacc acctcttgcg tccatcagga caaggtgtgc atgctaaagc gattacttct
     1501 gcaatttgtt tttcgttata ttttatgggt tgaccgggtc ttgctttgtc tttaagagca
     1561 ctttggagac cttcttcagc gtatctcttt tttattgtca aaacagtgtt cctacaaaca
     1621 cctaacgttt tagctatatc tatgcctctt ttttgctcgt ttgccagaag tagtatacgt
     1681 gctctggtta gttctcttgc atttttacgc ccttttctca caaagtccat aaggaaattt
     1741 acatcacaat cttcaagttt aaatttcagc ataaaaatat aaatatattc atagaatata
     1801 atgatgacca attatttaat ggtcgagaca ctagccgatt ctggcgagcc atttttgaca
     1861 cgtctatcct atacgtcttc gtgcgtatat ccaataaaca agctaaaagg cgtaaaatca
     1921 tcgtaaatac cattctaggc aatcttaggg
//
LOCUS       DATGBN010000090         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_550738, whole genome shotgun sequence.
ACCESSION   DATGBN010000090 DATGBN010000000
VERSION     DATGBN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550738"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VKL21_02000"
     CDS             complement(<1..283)
                     /locus_tag="VKL21_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HMB44572.1"
                     /translation="MTAIESAAREIIEQLLRNEIPDEKALNAAKKAASVRYKLSSLPG
                     NSRILAAARNDDEKQHVLERLQLKPVRTISGVAVIAAMTSPAPCPHGLCV"
     gene            543..1328
                     /locus_tag="VKL21_02005"
     CDS             543..1328
                     /locus_tag="VKL21_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB44573.1"
                     /translation="MTQSPTKRVDAVIMLGDMDTFEKTSQAYAESTARNIPVFYEIGN
                     HELDNKADLPQIRKLFASYSFNPLPGPDGSKETTYSFDVGDMHIVVLNEYWDGNNNGV
                     CDWKTPSGGNNADDSCFKYDGTDGGFIPDALFKWLENDLDRNTKKWTIVTGHEPLYPS
                     GRHVGDSLDEDTKNRDKLENLFVSKNVSAFIGAHTHFSKVITKDNIFHVDVGVSGIQT
                     ISGSGDSFASIIYTTVDSSGHFNIVHAKENPTWMTSKSINYSK"
BASE COUNT          475 a          264 c          315 g          447 t
ORIGIN      
        1 gaacgcataa gccgtgcggg cagggggcgg gggacgtcat ggccgctatt actgccaccc
       61 ctgaaatagt tctcacgggt tttaactgga ggcgttcaag gacatgttgt ttctcatcat
      121 cgtttcttgc agcggcaaga atccgggaat tgccgggaag ggatgaaagc ttatagcgga
      181 cgctggcagc cttttttgct gcgttgaggg ctttttcgtc ggggatctcg tttctaagaa
      241 gctgttctat gatttccctg gctgctgatt cgatagctgt catattttta tctgatattg
      301 atccgttgaa tcattaaggt tcgtgatata gaaccagcag cgattatata tactattcaa
      361 ccatttttcc ttacgaatcg gagagcaaaa gagtttcatt atctaattcc tgaatcaaga
      421 agccagtaaa gcaggttcaa acatgaaaat tattgatatc ttaatcatat ttatcggatg
      481 gaagaccaag ccaggaaaaa tcaaacagta aaaggaatct tacaatagat ttcaatcaga
      541 ttattacaca atccccaaca aaacgagtag atgccgtaat tatgcttgga gatatggata
      601 ctttcgaaaa aacctctcaa gcttatgcgg aatcaacagc aagaaatatt ccagttttct
      661 atgagatagg aaaccatgaa ttagataaca aagctgatct tccccaaatt aggaaattat
      721 ttgcatccta ctcctttaat cctttgcctg gacctgatgg ttctaaagaa acaacatatt
      781 cgttcgatgt aggagatatg catattgtgg tattaaatga atactgggat ggaaataaca
      841 atggagtatg cgactggaaa actccatccg gcgggaacaa tgcagatgat tcatgcttta
      901 aatatgatgg tactgatggt ggattcattc cggatgcact tttcaaatgg ttagaaaatg
      961 acctggatcg caatacaaag aaatggacta ttgttaccgg tcatgagcca ttatatccaa
     1021 gtggaagaca tgtgggggac tcacttgatg aagatacaaa gaaccgcgat aaacttgaaa
     1081 atttattcgt ctccaaaaat gtatcggctt ttattggtgc acacactcat ttttcaaagg
     1141 tgatcacaaa agacaatata tttcatgtag atgttggagt aagtggaatt caaacaatat
     1201 cgggctctgg tgattcgttc gcctccatta tttatactac agtggattcc agcggtcatt
     1261 ttaatatagt acatgccaaa gaaaatccca catggatgac gtcaaaaagc attaattata
     1321 gcaaatagcg gacgcagtaa ggatttatta ttcctttatt cgattaatgt accaggaatc
     1381 tttttaagca tgtactcttg ttaattgagg gtgtttagaa tgaaagataa aaacatactt
     1441 ttggtaggaa ttataatctc tctattgttt ttgagcagct ttgcattatt aggaaaaaac
     1501 a
//
LOCUS       DATGBN010000091         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_279029, whole genome shotgun sequence.
ACCESSION   DATGBN010000091 DATGBN010000000
VERSION     DATGBN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279029"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..426
                     /locus_tag="VKL21_02010"
     CDS             52..426
                     /locus_tag="VKL21_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1699 family protein"
                     /protein_id="HMB44574.1"
                     /translation="MLTSWRSTGIVHMAFRPSNKDILALVEACPKIEVIQLPKSYRRT
                     VSKSIEMFLGMQRIQLIEGDVWGHRKDINEYYSIPSSVIEKIKELKSEGTPAERIEEK
                     VSRESKLDPEMVAYILNKESLA"
     gene            758..1000
                     /locus_tag="VKL21_02015"
     CDS             758..1000
                     /locus_tag="VKL21_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMB44575.1"
                     /translation="MGGVSIKNRCRTDITVDILRVAMNGAKKTHIVSEVNLNFNIAQK
                     YLEMLNEKELIRHEKGLFITTDKGKVFQEMAKELKL"
     gene            1236..1577
                     /locus_tag="VKL21_02020"
     CDS             1236..1577
                     /locus_tag="VKL21_02020"
                     /inference="COORDINATES: protein motif:HMM:NF021503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2227 family putative metal-binding protein"
                     /protein_id="HMB44576.1"
                     /translation="MKGMSHELINFVILFLLIPIFFLFSIQGHNTIYFSFGWVIGTLY
                     LSPDLDADYSRPLKRIGNLKYLFWFTRHRGTLHNPVFWGCLFLILAFLGHAWMGAGLF
                     GAALVHILADK"
     gene            1810..>1981
                     /locus_tag="VKL21_02025"
     CDS             1810..>1981
                     /locus_tag="VKL21_02025"
                     /inference="COORDINATES: protein motif:HMM:NF020764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HMB44577.1"
                     /translation="MIDQTLRVFKELNSKDFRILTGIEIGKKHFEWVPIDELNKYTKL
                     PFDKLEYRIRKLV"
BASE COUNT          628 a          315 c          399 g          639 t
ORIGIN      
        1 tcccaatata tatagctctt ttattatttt ggaaatctga tgatataatc actgcttacg
       61 agttggcgaa gtactggtat tgttcacatg gccttcaggc cttctaacaa ggatatcctt
      121 gcattggttg aagcctgccc gaaaattgag gtaattcagt tacctaaatc ttataggcgc
      181 acggtttcga aatccattga gatgtttctt gggatgcaga gaatccagct tatagaagga
      241 gatgtctggg gtcacaggaa ggacattaac gaatattaca gcattccatc ctcggttatt
      301 gagaagataa aggaattaaa atctgaggga acacctgctg aaagaattga agaaaaagtc
      361 tcaagggaaa gcaaactgga tcctgaaatg gtcgcttata tcctgaacaa ggaatctctt
      421 gcctaaattc gggtaggata aaccatgtta gtaggcgcct cactggagat tagacagccg
      481 tatgtattca atctttcaat agtccttatt tgcgatctat tctctagtta aaaacttgtt
      541 attattttcc gggtttttga ttggtggcag ttaaatgcca gtgtgattat tactaaattt
      601 tgacaatcca gtattattat gatttgtggt tttatataaa aacattatta tataaaacgg
      661 aagaattggt atctaaagtc tttaatacct actaaaagac aatatttaat atatttttag
      721 agttagaatt cttgcaaaga cgatttttga atccaatatg ggaggagtca gtattaagaa
      781 caggtgcagg actgatatca ccgtagatat tttaagagta gcaatgaatg gggcaaagaa
      841 aactcatatt gtatctgagg taaatctaaa ctttaatatc gctcagaaat atctagaaat
      901 gttgaacgaa aaagaactca tcagacacga aaaagggctt ttcatcacta ctgataaagg
      961 aaaagttttc caggaaatgg ctaaggaact caagctttaa actgctttcc ttttatctct
     1021 tttaaaattt ttattgtttt ttcctggttt actgatttta gatctggagg tcattgatca
     1081 aagttgaaaa aaaagtatat cccctattat ggaaaaaata tagagagatt tagagagatt
     1141 attagactta tgggtttcat taactcacgg acactaacat ttctcgttca atagtataaa
     1201 ccctactgaa ttaatttgtt atatggagaa ggattatgaa aggaatgtct catgaattaa
     1261 taaacttcgt catcctcttt ctcctcatcc ccattttctt tttgtttagt attcaaggtc
     1321 acaacaccat atatttttca tttggctggg tgataggtac tctctattta tctcctgacc
     1381 ttgatgcaga ttatagcaga ccccttaaaa ggataggaaa tttgaaatat ttattttggt
     1441 ttacgaggca tcgaggtact cttcacaatc cggttttttg gggttgtctt tttctcatat
     1501 tggcattttt ggggcatgca tggatggggg ctggattgtt tggggctgcg ttggttcata
     1561 ttctggctga taaataatat cttttattca ctagcaaata aagttcgagg cgatacacac
     1621 atctgatctt ccataaggtg aaaaatggaa cctaacgcaa gggtaaggac tttgatatct
     1681 ccccttttct cgtataatag tttgcccaac aagggaaacg atttgctcaa caagagtagg
     1741 ggagtgaaaa agaagaccat gagcctggga gctattattt aaatatcgag gattatattt
     1801 aatatcaaga tgattgacca aacgctaagg gtttttaagg agcttaactc gaaggatttc
     1861 aggatcctta ctggcatcga aatcgggaag aagcattttg aatgggtgcc catagatgaa
     1921 ctgaataagt acaccaaatt gccctttgat aagctggaat acaggataag gaagcttgtc
     1981 c
//
LOCUS       DATGBN010000092         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_46104, whole genome shotgun sequence.
ACCESSION   DATGBN010000092 DATGBN010000000
VERSION     DATGBN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46104"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..637)
                     /gene="cyaB"
                     /locus_tag="VKL21_02030"
     CDS             complement(74..637)
                     /gene="cyaB"
                     /locus_tag="VKL21_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HMB44578.1"
                     /translation="MIEIEVKAFVEDPKKMERSIIELGATPIGIESQSDTYYNSPYRD
                     FGKTDEALRIRVQDGKSILTYKGPKIDLVSKTRKEVQTEILDTDSMGNILSSLGFFPV
                     ATVSKKRKNFRMGDFTISLDEVRNLGNFIEVEITVKDPRNHHEKVESIFKFIAKLGLN
                     RESTIRRSYLEMILEKNEIEICSPPVE"
     gene            741..>2626
                     /locus_tag="VKL21_02035"
     CDS             741..>2626
                     /locus_tag="VKL21_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HMB44579.1"
                     /translation="MVQSSIQAWEDFFKLYYWENILELARDYPEKRSLVIQFPDINRR
                     DMDLANELLEHPDKVIKHANEALSGIDLPADVVFTDTHVRIIKIPERIQIRELRSDNI
                     NKFIAVSGLIRKATEVRPKIRTAAFKCKYCEELTKLPQNEGKFVEPFQCEQCERKGQF
                     KLIHEESVFIDAQKLRIQESPDDLRGGEQPQTLDVDVDDDLAGLVSPGDRVVISGILR
                     SFQRSNREGKSTFFDLLLDGISIDIEDKEFEDIEISKEDVEEIIALGNDPKIYDKIIG
                     SIAPSIYGYEEVKEAMALQLFSGIAKYLPDGTRIRGDVHILLVGDPGVAKSQLLRYAV
                     KLAPRGIYTSGKSSTSAGLTATAVKDEFGDGRWTLEAGALVLADMGLACVDELDKMEP
                     DDRSSMHEAMEQQTISIAKAGIMATLKSRCALLGAANPKFGRFDRYEPIAKQINMPPA
                     LLSRFDLIFILTDEPSVNRDTAIAEHILKAHYAGELSVRRNNVINSGISQDEITKAME
                     IIQPVLEAEKLRKYIAYTKRNIFPIISDDARKHLIDFYLGLRKQGEAPDSPVPVTARQ
                     LEALIRLAEASARVRLSNVITTDDTNRIIRIVMASLKQVMTDPETGRLDADIVNVGMG
                     KSQR"
BASE COUNT          764 a          588 c          591 g          683 t
ORIGIN      
        1 cttcctgtat cctgggataa agtaaaccga acacgctcat actgtgctaa aaaagtggaa
       61 tcaatatata aaactattct actggaggcg aacaaatttc gatttcattt ttttcaagaa
      121 tcatttccag atatgatctg cggatagttg attctctgtt tagccccaat ttggctatga
      181 atttaaaaat gctttcaact ttttcatggt gattccttgg atccttaaca gtgatctcaa
      241 cttctatgaa attcccaagg ttacgcactt catccaggga aatagtgaaa tcccccatcc
      301 tgaagttttt tctttttttt gatacggtag caaccggaaa aaaaccaagc gatgaaagta
      361 tatttcccat actatccgta tccaggatct cagtttgcac ttcttttctt gtttttgaaa
      421 ctaaatcaat tttaggtccc ttataggtta aaatggattt cccatcctgc accctgatcc
      481 tcaaagcttc atcagtcttc ccgaaatcac ggtatggcga attataatat gtatctgact
      541 gcgattcaat cccgataggc gtagctccca attcaattat cgacctttcc attttttttg
      601 ggtcttccac aaatgccttc acttctatct ctatcatata tgtcaattta tctccccgtt
      661 atatctaatt gtttattttt tcctgtttgt agagtccttc tcgaaagatt gaaataccct
      721 tcgggctaaa gtagtaaaaa atggtacaat catctatcca ggcatgggaa gatttcttta
      781 aactctatta ctgggagaac atccttgaat tggcaaggga ttatcctgaa aaaagaagtc
      841 tagtcattca atttccagac atcaatcggc gtgatatgga tcttgccaac gagctcctcg
      901 agcatcccga caaagtaata aaacatgcaa atgaggcctt aagtggaatc gacctgcctg
      961 cggatgtcgt ttttaccgat acccatgtaa ggattatcaa aattccggag cgaatccaga
     1021 taagggaact ccgaagcgac aatatcaaca aattcattgc tgtctcaggc cttatcagga
     1081 aagcaacaga agtaagacca aagatcagga cagctgcttt caaatgcaaa tattgtgagg
     1141 aacttacaaa attgccgcag aatgaaggga aatttgtgga gcctttccag tgcgagcaat
     1201 gtgaacgtaa aggtcagttc aaactcattc atgaggaatc cgtatttatc gacgcccaga
     1261 aactgcggat acaggaatca cccgacgacc tccggggcgg tgagcagccc cagactctcg
     1321 atgtggatgt cgatgacgat cttgcaggac tggtctcacc cggggatcgc gtggtcatat
     1381 ccgggatatt gcgttcattc cagcgaagca accgggaggg aaaaagcact ttttttgatc
     1441 tcttgcttga cgggatatca attgatatag aagacaagga gttcgaggat atcgagatat
     1501 caaaagagga tgtcgaagag atcatagctc ttggaaacga tccgaaaata tacgataaga
     1561 taataggctc catagctcct tcgatctacg gttatgagga agtaaaggaa gccatggccc
     1621 ttcagctttt ctccggcata gccaaatacc tgcccgatgg cacgcgcatt cgcggagatg
     1681 tacatatact cctggtgggg gatcccggcg tggccaaatc ccagctgctg cggtatgccg
     1741 taaaacttgc accgcgcggc atttacacaa gcggtaaaag ctcaacatcg gcgggtctta
     1801 ctgccacggc cgtcaaggat gagttcgggg atggccgctg gacgcttgaa gccggcgccc
     1861 ttgtgcttgc agatatgggc ctggcatgcg ttgatgaatt ggacaagatg gaaccggatg
     1921 acaggagttc catgcacgaa gcaatggaac agcagaccat aagcattgca aaagcaggga
     1981 tcatggcgac attgaaatca cgctgcgctc ttctgggggc ggctaacccg aaattcggaa
     2041 ggtttgacag gtacgaaccc atcgcaaaac aaataaacat gcctccagcg ctcctttccc
     2101 gttttgacct tatctttatc cttaccgatg agccttcggt caacagggat accgcaattg
     2161 cggaacatat cctgaaagca cattatgcag gggaactttc agtcaggagg aataatgtaa
     2221 taaattcagg gattagtcag gatgagatca ccaaagccat ggagatcatc cagccagttt
     2281 tagaagcaga gaagctcagg aaatatattg catatacaaa gaggaatata ttcccgatta
     2341 tcagtgatga tgcaaggaaa catttgatcg acttttatct tggcctcagg aaacagggtg
     2401 aagctccgga ttccccggtt cctgtaacag cccgtcagct tgaagccctg atacgcctgg
     2461 ccgaagcaag cgcccgcgta cgcctgagta atgtcataac cacagatgat acgaaccgga
     2521 tcatccgtat tgtgatggca agcttgaagc aggtaatgac cgatccggaa acgggaaggc
     2581 ttgatgccga tatcgtcaat gtaggaatgg gtaaaagcca gcgaga
//
LOCUS       DATGBN010000093         1745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_434284, whole genome shotgun sequence.
ACCESSION   DATGBN010000093 DATGBN010000000
VERSION     DATGBN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434284"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VKL21_02040"
     CDS             complement(<1..360)
                     /locus_tag="VKL21_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMB44580.1"
                     /translation="MLSKITVFDGVRCIGGNKIHLEFDGRGVFFDFGMNYKKIGDFYE
                     EFLSPRSSRGIHDLLFMGMIPNINCYRKDLMPGDLDMSCAQKVRVDAVFLSHAHMDHV
                     GNVGLLDVNIPIVSSQMT"
     gene            590..1534
                     /locus_tag="VKL21_02045"
     CDS             590..1534
                     /locus_tag="VKL21_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMB44581.1"
                     /translation="MFKNIIDEISPAKKAWETMRQKSPAKKAWDTMRRQKGAQIGAQK
                     IQKNQWEQEKIDHLYILESKTKKEACIVCSDNRKKVLQDHHIKPDDSHTVKLCANCHD
                     VVRRGTLNDLIEINYPKEDPLDNLIDSAFKVAVIAHQNQTKKGTNIPYIVHPISVAQI
                     LLQAGFPEEIIAAGILHDTVEDTKVTLDFIRDRFGDRVADIVNGCSEPDKSLPWKERK
                     MHTIEFLKTASLEVRIVACADKLLNIRTIASDYEKIGDKLWERFTETRKEEHEWYYIG
                     VAASLCEQPGTKDMAIFQQLNKEVETFFDKIKKDNRIG"
BASE COUNT          594 a          338 c          302 g          511 t
ORIGIN      
        1 tgtcatctgg gatgacacga tcggtatatt tacatccagt aagccaacat tcccaacatg
       61 atccatatgc gcgtgggaca ggaacactgc atcgaccctg actttttgag cgcaagacat
      121 atcgagatct cctggcatca agtccttcct gtagcagttg atattcggga tcatccccat
      181 gaatagcagg tcatgaatgc cccttgatga tctagggctc agaaattcct catagaaatc
      241 ccctattttc ttataattca ttccaaaatc aaaaaaaaca cctctgccat cgaattccag
      301 gtggatttta tttccaccta tacaccttac tccatcgaaa accgtaattt ttgaaagcat
      361 gataccaccg tagtattact tatatattga gattatatat atttatgata tctgaatact
      421 cgcatgctca catgatcgaa taaacccaat tattttatta ggtttaagcc cagtatccac
      481 tagcttcact tatctgaacc tttaactcaa ctttgtttgt caatctctga aaattgatca
      541 ttaattaatt taagatagta ttatatattc attaattata ttacttcaaa tgtttaaaaa
      601 tatcatagat gaaattagtc ctgctaaaaa agcatgggag acaatgagac aaaaaagtcc
      661 agcaaaaaag gcatgggata caatgcgacg acagaaagga gcacagatag gtgcacagaa
      721 gatccaaaaa aaccaatggg aacaagaaaa aatagatcat ctatatatcc tggaaagtaa
      781 aacaaaaaaa gaagcctgta tcgtatgtag cgataataga aaaaaagttc ttcaagacca
      841 tcatatcaaa cccgatgata gccatactgt caaattgtgt gcaaactgcc acgatgtagt
      901 tagacgtgga acattaaatg atttaataga aataaattat cccaaagaag atccattaga
      961 taatttaatc gattccgcct ttaaagtagc cgtcatagct catcaaaacc agacaaaaaa
     1021 gggcaccaat attccatata ttgtgcatcc aatttcagta gcccaaatac ttttacaggc
     1081 aggctttccc gaagaaatta ttgcagcagg catactccac gatacagtag aagatacgaa
     1141 agtgacattg gattttattc gtgatagatt tggtgacaga gttgctgaca ttgtaaatgg
     1201 atgttcggag ccggacaaat ccttgccatg gaaagaacgc aagatgcata cgatagaatt
     1261 cttaaaaacc gcttctctgg aagttcgaat tgtagcgtgt gctgacaaac tcttaaatat
     1321 cagaactatc gccagcgatt atgaaaaaat tggtgataaa ttgtgggaac gttttactga
     1381 aacgagaaaa gaagaacatg aatggtatta tataggggtt gccgcaagtt tgtgcgaaca
     1441 acctggtact aaggacatgg caatatttca gcagctaaat aaagaagttg aaactttctt
     1501 tgataaaata aagaaggata atagaattgg ataaaatctt aaatactcag tcccatatta
     1561 ttagaatcca gatcatccgc ttcttctgct tctttttgtt tgctgtgtcc attaattctt
     1621 caatccggcc tgagattcaa tttattcttt tccttcgtcc tctttctcta tctctgctat
     1681 ccattcatcg tttaattgtt tttccagaac aggtattgcc cgttcttggt ctgtgatcca
     1741 gtcca
//
LOCUS       DATGBN010000094         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_318098, whole genome shotgun sequence.
ACCESSION   DATGBN010000094 DATGBN010000000
VERSION     DATGBN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318098"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VKL21_02050"
     CDS             <1..621
                     /locus_tag="VKL21_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HMB44582.1"
                     /translation="MMSGNGPGSGMMGYGNGANASYGSMMSGNGTGHCGAGYTDSGYG
                     ANATPITIEKAKESVEQYLTSTGNSDLKLSEVIEFDNNFYAGVKEQSTGAHAFELLVN
                     KYTGAVFPEMGPNMMWNTKYGHMNRNIPAQTNISEEQALKNAQDYLDKALPGTKVGNA
                     DAFYGYYTIEVQKDSNIYGMLSVNSNTGAVWYHNWHGAFVEILEVG"
     gene            717..1010
                     /locus_tag="VKL21_02055"
     CDS             717..1010
                     /locus_tag="VKL21_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMB44583.1"
                     /translation="MDKKSLAEMLNALGNEHSLTILAILAESECYVSELAKEVGISRP
                     LLYLHLKKLENVDLVESEIRHFEEPPYTKKFYKAKNFELVLSLSKIKEIVKKE"
     gene            1015..1197
                     /locus_tag="VKL21_02060"
     CDS             1015..1197
                     /locus_tag="VKL21_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44584.1"
                     /translation="MSSMMDAMFGTTTQWPIYTLLIVVVIFGLISVYLTYMMLMELKE
                     SRKSLEKMEKLLKSVE"
     gene            1202..>1816
                     /locus_tag="VKL21_02065"
     CDS             1202..>1816
                     /locus_tag="VKL21_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMB44585.1"
                     /translation="MVLQESDKETIRKKFVGLADDVELIVFSQEIESQFCKETKELML
                     ELGALSKKLHVNVYDFVKNGDDVLKYKIKKIPAIAIVGKIDYGIRYYGVPAGYELPIL
                     IDTIIDVSRGKTSLTDALKTRLAEIRKPVHLQVFVSPTCPYCPKAARIAHQFSIENEN
                     ISSDVIEMVEFPYLAQRYSVMSVPHIVINENTSFIGAHPPEIFIE"
BASE COUNT          595 a          316 c          416 g          489 t
ORIGIN      
        1 atgatgagcg gtaatgggcc tggaagtgga atgatgggct atggcaacgg agcaaatgct
       61 agttacggca gtatgatgag cggtaatgga actggacact gcggcgcggg ctatactgat
      121 agcggttacg gggcgaatgc caccccaata actattgaga aagcaaaaga atctgtagag
      181 cagtacctta cttccacagg taattccgac ctcaaacttt ccgaggtcat agaatttgat
      241 aataacttct atgccggagt gaaagagcaa agcacaggag cacacgcatt tgaattgctg
      301 gtgaacaaat atacaggcgc tgtattccct gagatgggtc ctaacatgat gtggaataca
      361 aagtacgggc atatgaaccg gaatatccct gcacagacca acataagcga agaacaggca
      421 ctcaagaatg cacaggatta cctcgataag gcgctgcctg gaacaaaagt gggtaatgct
      481 gacgccttct atgggtatta cactattgag gttcaaaaag atagcaatat ctatggtatg
      541 ctcagcgtta acagcaacac aggcgctgtc tggtatcata actggcatgg ggcttttgta
      601 gaaatactgg aagttgggta agcttccggc atttttatat taaaaattga ggctgaggca
      661 atattaatcc cttttattta tatacatttg aattctttta gtcaaccggt gaaaaggtgg
      721 ataagaaatc acttgcagaa atgctaaatg cgcttggcaa cgagcatagc ttaacgattc
      781 ttgcaattct tgcagaaagt gaatgctatg tttcagagct tgcaaaggaa gtcgggattt
      841 cacgtccgtt actgtatctt cacctcaaaa aacttgaaaa tgtagatctt gtggaaagtg
      901 agatccggca ttttgaagag ccgccgtata caaagaaatt ttataaagca aagaattttg
      961 aattagtact ttcattaagt aaaatcaagg agatagtaaa aaaggagtaa aattatgtcg
     1021 tcgatgatgg atgcaatgtt cggtacaacc actcagtggc ctatatatac ccttcttatt
     1081 gttgtcgtaa ttttcggtct aatatcggtc tatttgacct acatgatgct catggagctt
     1141 aaagaatcaa ggaaatcact ggagaaaatg gaaaaactct taaaatcggt agagtgatat
     1201 tatggtgctc caggagagcg ataaggaaac tatccggaaa aaattcgtgg gtcttgcaga
     1261 tgatgtggaa cttattgttt tttcacagga aattgagagt caattctgca aggaaactaa
     1321 agaactgatg cttgaattgg gggcattatc aaaaaaatta catgtaaatg tatatgattt
     1381 tgttaaaaat ggggatgatg tcctgaaata taaaattaag aaaatccccg ccatagcaat
     1441 agtaggaaaa atagactatg gcataagata ttacggcgta ccggcgggat atgaattacc
     1501 catcttgatc gacacgataa ttgatgtttc caggggaaaa acatcattaa ctgatgcatt
     1561 aaagaccagg ctggcagaaa taagaaaacc cgtgcatttg caggtattcg tttcgcctac
     1621 ttgcccatac tgtcctaaag cggcaaggat cgcgcaccag ttttccattg aaaatgaaaa
     1681 tattagttct gatgtaatag aaatggtgga atttccgtac cttgcccaga gatatagcgt
     1741 tatgagtgtt cctcacattg tgatcaatga aaatacttcc tttataggtg cacaccctcc
     1801 ggagatattt attgaa
//
LOCUS       DATGBN010000095         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_240504, whole genome shotgun sequence.
ACCESSION   DATGBN010000095 DATGBN010000000
VERSION     DATGBN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240504"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VKL21_02070"
     CDS             <1..206
                     /locus_tag="VKL21_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44586.1"
                     /translation="ATSNMDLTRAFGVTQNPILEHLKWEMIVDTDASMEKLNKLNEIA
                     KERCPGYYCVTNEIFPEIKIIKQ"
     gene            222..1376
                     /locus_tag="VKL21_02075"
     CDS             222..1376
                     /locus_tag="VKL21_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HMB44587.1"
                     /translation="MAKNDIVLRSLDLFKDAFPGRINQILKSKEEGKKIIGTLCLYVP
                     DELIYACGADRVILCGGRSAPIEAAEEYLPRNLCPLIKSSFGSVIDQKCSKTKSCPHF
                     GLVDLIVAEATCDGKKKMYELLDKYIPTHVMDLPQKPENQNALEYYIKELESLKDVLE
                     KLTGNKITDVLLRNEIKSANETRRLLHALYELRKRDSPPIRGTEMLKVLQQMHFLAPD
                     EFRKNLVLLFDELKVKKRESSGPRIMISGCPMASGNMKVPEIIEERGGLIVVEESCTG
                     TRAFWDLVDENKEPMRALARRYLKIPCACMYPNERRINQILKLAGDFNVDGVVYYTLQ
                     FCHGYNVEKYRVGEALKKSNIPLLSIETDYSGADTEQIKTRVDAFLEMLE"
     gene            1373..2128
                     /locus_tag="VKL21_02080"
     CDS             1373..2128
                     /locus_tag="VKL21_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HMB44588.1"
                     /translation="MISIGIDAGAATTKAVVLRENEIAGYTIIPTGFDFRKAGEEAYR
                     EVLSNCGIGESEVGKILATGYGRSSIGFADKTISEITAHARGVGYLIPAAHTIIDIGG
                     QDSKVIITENGKVVDFLMNDRCAAGTGKFLEYTAKALEISLNELGTLALVSKNPAKIS
                     SMCTVFAESEVISLRAQKTKKEDIAAGLIESIILRTIVMVKKLGIKPEVAFVGGVAKN
                     PGVVKAFEKALDTRVQLPEEPQITGALGAAMLI"
     gene            complement(2132..2479)
                     /locus_tag="VKL21_02085"
     CDS             complement(2132..2479)
                     /locus_tag="VKL21_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMB44589.1"
                     /translation="MQKESKRCTEYEGDICLCSIEGIMGILSKKWALLIISVIGNNQK
                     LRYNELEKKLDKISPKTLADRLKELENANIIKRESFAEIPPRVEYSLTKEGTELRDAV
                     MPLIKWVSFRDAQ"
     gene            2978..3151
                     /locus_tag="VKL21_02090"
     CDS             2978..3151
                     /locus_tag="VKL21_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HMB44590.1"
                     /translation="MIFWILVIIGLVLLIKYLWGGGRTKRGKESALEILKKKYARGEI
                     NKEEFEAKKKDLI"
     gene            3250..>3338
                     /locus_tag="VKL21_02095"
     CDS             3250..>3338
                     /locus_tag="VKL21_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMB44591.1"
                     /translation="MIATDGSEHTEKAITHAIELAKLTGAQLHA"
BASE COUNT         1112 a          612 c          761 g          853 t
ORIGIN      
        1 aggctacgtc gaacatggat ctcacaaggg cttttggagt tactcagaac ccgatcttag
       61 agcatcttaa atgggaaatg atagtcgata ctgatgcaag catggaaaag ctgaataaat
      121 tgaacgaaat cgcaaaagaa agatgccccg gctactattg cgtaacgaat gagattttcc
      181 ctgaaatcaa gatcataaag caataacaga tgaacgatca gatggcaaaa aatgacattg
      241 ttctgaggag tttagacctt ttcaaggacg cttttcccgg aaggataaat caaatactca
      301 agagtaaaga agagggaaaa aagataatag gaacactctg cctttatgtc ccggacgaac
      361 tcatctatgc atgcggcgcg gaccgtgtaa tactatgtgg tggaagaagc gcccccattg
      421 aagcggcgga ggaatatttg ccacgaaacc tctgccctct gattaaatcc tccttcggct
      481 ctgtaattga ccagaaatgc agtaagacta aatcctgtcc tcattttggt ctggttgacc
      541 tcatagttgc agaggctaca tgtgatggga aaaagaagat gtatgaactt ctggacaagt
      601 atatcccaac ccatgtcatg gatcttccgc agaagcctga aaatcagaac gcactggaat
      661 actatataaa agaactggaa agcttaaaag acgttctgga aaagctgaca ggcaataaaa
      721 ttactgacgt cctactcagg aatgaaataa agtctgcaaa tgagacaaga agactgctcc
      781 acgcactcta tgagttaagg aaaagagact caccgccaat taggggaaca gaaatgctaa
      841 aggttcttca gcaaatgcat tttctggctc ccgacgagtt cagaaagaat cttgttttac
      901 tgttcgatga gctaaaagta aagaaaaggg agagttccgg gccgaggata atgatatccg
      961 gatgcccgat ggcatcaggg aatatgaagg ttccagagat catagaggag cggggcgggt
     1021 tgattgtggt ggaggaaagc tgcactggaa caagagcctt ctgggattta gtggatgaga
     1081 ataaggagcc aatgagagcc ctggccagga ggtatctgaa aatcccctgt gcctgtatgt
     1141 atccaaatga aaggaggatc aatcagatac taaagcttgc cggggatttc aatgttgacg
     1201 gggttgttta ttatacactt cagttctgcc acggatacaa tgttgagaaa tacagggttg
     1261 gggaagcttt gaaaaagtca aacattcctc ttctctctat agaaacagac tacagcgggg
     1321 ctgacactga gcagataaag acacgagtgg atgcttttct ggagatgctg gaatgatatc
     1381 aattggaatc gatgccgggg ctgcgaccac caaggctgtt gtacttcgtg aaaatgaaat
     1441 tgcaggatac accattatac ctacaggttt tgattttaga aaagcgggag aggaagcata
     1501 cagggaagtt ctctcaaatt gcggcatagg cgaaagcgag gtaggaaaaa ttctggctac
     1561 aggatacggg agaagcagta ttggatttgc agataaaact atcagtgaaa taacagctca
     1621 tgccagaggt gtcggctatc ttatcccggc agcccacacg ataatcgaca tcggtggcca
     1681 ggatagcaaa gtgatcatca cagagaatgg taaagttgtg gactttctga tgaatgacag
     1741 atgcgcggca gggaccggta agtttctgga atatacagca aaagcgcttg aaatatcgct
     1801 gaatgaattg ggaacacttg cactggtttc aaaaaatcca gcaaagatca gcagcatgtg
     1861 taccgtattc gcggaatccg aagttatcag cttaagagcc caaaagacta aaaaagaaga
     1921 tatagctgcc ggtcttattg aaagtattat tctgcgaacc attgtaatgg ttaaaaagct
     1981 aggaataaaa ccagaagtgg cttttgtggg cggagtggca aaaaatcctg gagtagtaaa
     2041 ggcttttgaa aaggccttag ataccagagt acaattgcca gaagaacccc agataacagg
     2101 cgccttgggg gctgcaatgc ttatttaaaa attactgagc gtccctaaaa gaaacccatt
     2161 ttatcaaagg cattacagca tctctcagtt ccgttccttc ttttgttagc gagtattcca
     2221 cccttggagg tatttcagca aatgattctc ttttgattat gttcgcattc tccagttctt
     2281 ttaacctatc ggctaacgtt tttggactta tcttatccag ctttttttca agttcgttgt
     2341 agcgcaattt ctggttattc ccaataacac tgatgatcaa taaagcccat tttttactga
     2401 ggatgcccat gataccttca atagaacaca aacatatatc gccctcatac tctgtgcatc
     2461 ttttactttc tttttgcata gcgacttcct ttttcaatag cagcttttta actttaaact
     2521 tgatagttta aatactttat taactgaagt aactattaag aaaaaaggag gtgaaaaaga
     2581 tgtgccgaca atgccttccc attacaataa ataaagaaca accgatgaat gataatacaa
     2641 aaagtagaaa taaagaaaca gttagcgaag aacacgaaca aatcttgaaa aaaatttaaa
     2701 atgtatctgc ccctgtttat gacagaggca ataatttatt catataaaaa aggcgataaa
     2761 atgaaaataa ttatcatctt tatgctgtgg ctgcattatc tggcgactac gatgtggatt
     2821 ggagggatga ctttcaatat actggtgttt cgcccttcaa tggtagcaat tgaccaaaac
     2881 cagcgcctag tgctcggaac tacggtatta aagcgtttca ttatttttgc ctggttgagc
     2941 atggcagtgg tgatccttag gttactggat ttttgggttg atattctgga tactggtaat
     3001 catagggctt gtgctgctca tcaaatacct gtggggaggc ggcagaacta aaagaggaaa
     3061 agaatcagcg cttgaaatcc ttaaaaaaaa gtatgctagg ggagagataa ataaagaaga
     3121 atttgaagcg aaaaagaaag acctgatata aataagaact taaagggaaa accacttaaa
     3181 taaaaaggaa gattttcagt ttatacatct ttaaggagaa aaattacgaa taagctttat
     3241 aaaaaaatcc tgatagcaac agatgggtca gagcacactg aaaaagcaat aacccatgca
     3301 attgaacttg caaagctcac gggagcgcag cttcatgc
//
LOCUS       DATGBN010000096         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_318174, whole genome shotgun sequence.
ACCESSION   DATGBN010000096 DATGBN010000000
VERSION     DATGBN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318174"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VKL21_02100"
     CDS             complement(<1..326)
                     /locus_tag="VKL21_02100"
                     /inference="COORDINATES: protein motif:HMM:NF021416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HMB44592.1"
                     /translation="MRIGIILHGPEIVDMGSARKIIDIFTKEHDVTARLGGTMGRTAV
                     LDAGLEDLIDISQGRTPSETINAIKDDIDLAILLNHGKTLDTGRYFGGIVASRLDSSV
                     PLVHIE"
     gene            complement(645..1796)
                     /gene="ftsZ"
                     /locus_tag="VKL21_02105"
     CDS             complement(645..1796)
                     /gene="ftsZ"
                     /locus_tag="VKL21_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB44593.1"
                     /translation="MESFVQDALKYKEKEQLTRQAACGSSEEMDEFGTPKIVVVGCGG
                     GGNNTVNRLYNIGVAGADTIAINTDKIHLDITQADKKILIGKSITRGLGAGGFPEVGR
                     RAAELARGTLEEVLKDANLVFVTAGMGGGTGTGVAPVVAQVAKDQGAIVIGMVTSPFK
                     VERSRMFKAEEGLDELRKAADTVIVLDNNRLLDYVPNLPIDQAFSVMDQLISETVKGI
                     TETITQPSLINLDYADVRAIMKGGGLAVMLVGEAKGQDKAKEVVRAALNHPLLDVDTK
                     GATGCLLHITGGTDMSLHEAEQIASSLTYELDSHANVIWGARVKKEYEGKVRCMAIMT
                     GIHSAQIMGPKADSMAAGNISSVEKRIKVAIDLTRNNSRNRGSIIDQIL"
     gene            complement(1830..2054)
                     /locus_tag="VKL21_02110"
     CDS             complement(1830..2054)
                     /locus_tag="VKL21_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HMB44594.1"
                     /translation="MQRVTLRLPEQQLKMIDILVEYGEFPSASEAIRTAIRDLIDQRS
                     EKLVGRMQLFDKAQEQSKNAATFLRLKEEH"
     gene            complement(2290..>2676)
                     /locus_tag="VKL21_02115"
     CDS             complement(2290..>2676)
                     /locus_tag="VKL21_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HMB44595.1"
                     /translation="LDLVVIGGEWGEGRRTKFIGSYLLACRDPYSEKFLPIGRVATGI
                     SDEQLEDLTKLFKDMIILENGIHVDIEPKIVFEVAFEEIQKSPNYESGYALRFPRLVN
                     VRSDKSIEDADSIERIEELYIGQKGR"
BASE COUNT          660 a          636 c          494 g          886 t
ORIGIN      
        1 ctctcgatat gtacaagagg gactgaactg tccagtcttg aggctactat cccgccaaaa
       61 tagcggccgg tatccagggt ttttccatga ttaaggagta ttgcaagatc aatatcatcc
      121 ttaattgcat tgatggtctc acttggcgtg cgcccctgag aaatatcaat aaggtcttca
      181 agaccggcat ccagtactgc cgttcttccc atagttcctc caagtcttgc ggttacgtca
      241 tgttctttgg tgaatatgtc aattatcttc cgggcagaac ccatatccac gatttcagga
      301 ccgtgaagaa ttattcctat gcgcatgctt atagaattat attctattcc agattatcag
      361 atcgtctgtt aagaaattta tggactatga aaattacgat ccagaaaatc aatgctccta
      421 ttatcgcacc tttccaatcc agctctttat tgtgcaaaag atatttaaga attataaata
      481 taacaaaata tgttaatgat gcggaagaag ccgtaaaata tgggttcgtt ttttccatta
      541 gctcaacgta ttaatttagt atgtcaaata tctttccttc actgagaata aacaaaaaaa
      601 aagataatcc gacaataatc ttgtcggact tgcattttat aatatcacag gatctggtcg
      661 attattgaac ctctgttcct gctattattc cttgttaaat ctattgccac tttgattctc
      721 ttttcaactg aagaaatgtt tcccgctgcc atgctatcgg cctttggtcc catgatctgt
      781 gctgaatgga tacctgtcat gatcgccatg cagcgcactt ttccttcgta ttccttcttt
      841 actcttgcac cccagataac gtttgcatga gagtcaagtt cataagtcag ggatgaagcg
      901 atctgttcag cttcatgcag agacatatca gttccacctg ttatatggag caagcaaccg
      961 gttgcgcctt ttgtatccac atcaagcagt gggtgattca aggcagctct tacgacttcc
     1021 tttgccttat cctgtccttt tgcctcaccg acaagcatta ctgcaagtcc tccgcccttc
     1081 attattgccc tgacatcagc gtagtcaagg tttatcaaag acggctgggt gatggtttca
     1141 gtgatacctt tcacggtctc tgatatcaac tggtccatta ctgagaatgc ctggtcaata
     1201 ggcagattcg ggacataatc aagcagcctg ttgttatcaa gaactattac ggtatctgct
     1261 gcttttctta attcatccag gccttcctct gccttgaaca tccttgagcg ctctaccttg
     1321 aacggacttg tgaccattcc gatcacgatc gctccctggt cctttgcgac ctgtgccacg
     1381 acaggagcta cacctgttcc tgttccaccg cccattcctg ctgtgacgaa tacaaggttc
     1441 gcgtctttaa gtacttcttc aagtgttcct cttgcaagtt ctgccgctct tcttccaact
     1501 tccggaaaac cgcctgcgcc caatcccctt gttattgatt tgccgattaa gatcttctta
     1561 tccgcctgtg tgatatcaag gtggatctta tctgtgttga ttgcgatagt atctgcgcct
     1621 gcaacgccaa tattataaag acggttaact gtattattgc cgcctccgcc gcacccgact
     1681 actacgatct tgggtgttcc gaattcatcc atctcttcag atgagccaca tgctgcctgt
     1741 ctggtcaact gttccttttc tttgtatttt aatgcgtctt gtacaaatga ttccatattc
     1801 atcccctcag atgatttttt ttattccttc tagtgttcct cttttaagcg taagaatgtc
     1861 gccgcgttct ttgactgctc ttgcgcctta tcaaataatt gcatcctgcc aaccaatttt
     1921 tcacttcgtt gatcgattaa atcccttata gccgtcctga ttgcttcgct tgcggacgga
     1981 aattctccat actcaactaa tatgtcgatc atttttaatt gctgttccgg gagccttagt
     2041 gttactcttt gcattttggt aactccctgt gggaggattg atgaaccata agatttttca
     2101 tatttttaga caggttcatc atagttgctt acaattgtac gccttttgtg cgacccttgt
     2161 atgacgtttg tctgacacat tgtctgactt gaagatatat ataagtttcg ttatgggaat
     2221 atcatcatgg aatattgatt ttgtttcaag ttgaattcaa aggcaaataa aaataatatt
     2281 aaggtatatt tatctcccct tttgcccgat ataaagttcc tcgatccttt ctatagaatc
     2341 agcgtcctca attgatttat cgctcctgac attgacaagc cttggaaagc gaagtgcata
     2401 tccggattcg taattcgggc tcttctggat ttcctcaaat gccacttcga acacgatttt
     2461 tggctcaatg tcaacatgaa ttccattttc taagattatc atgtccttga acagttttgt
     2521 aagatcttca agttgctcat ccgatatacc ggtggccacc cgtccgattg gaaggaattt
     2581 ttcagaatat gggtcacggc aggccagcaa ataagaacct atgaatttgg ttcgtcttcc
     2641 ttcgccccat tcccccccta taaccacaag gtcaag
//
LOCUS       DATGBN010000097         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_163066, whole genome shotgun sequence.
ACCESSION   DATGBN010000097 DATGBN010000000
VERSION     DATGBN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163066"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /gene="trpD"
                     /locus_tag="VKL21_02120"
     CDS             <1..996
                     /gene="trpD"
                     /locus_tag="VKL21_02120"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HMB44596.1"
                     /translation="QSLTTEEAELAISKIFTDATDIQISAFLTAMKMKGETPSEIAGL
                     AMGMKKAANIIDPQVKGTLIDTCGTGGDSTGTINVSTGAALVAAGAGVPVAKHGNYSI
                     TSKSGSADVLRELGVKIDAPPEQVEKTIEKVGIGFMLAPVFHPSMKRVAQIRRDLGFR
                     TVFNILGPLTNPANAKAQVIGVFDSSLCEPIANVLHILGTKRALVVHGSGMDEISTTG
                     ETQIVELNGGKITKYTLTPEKLGIKRAANGDIAGGTPEENARDIVEALKGKKGPKRDI
                     IAVNAGAAILVSGKAENLADGINLANQAIDSGAAMSKLKELVNAAGEPEKLARFL"
     gene            1012..1656
                     /locus_tag="VKL21_02125"
     CDS             1012..1656
                     /locus_tag="VKL21_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HMB44597.1"
                     /translation="MKVKICGIKSERDLAMAIKAGADAAGFITEVPVDSPRKITLTEA
                     SRLIAHVPVFVTSVLVIMPKNAYEAIEMINAARPTAVQIHNSLPINELKEIRQTGVKL
                     IKTIPVPIHADTRMLLDIVKGLSGIVDAVLLDTALDGKTGGTGVVHNWELSSNIVRKA
                     GLPVILAGGLNPTNVMDAVKCVRPYAVDTASGVETEGKKDENKLIDFIKNARML"
     gene            1653..3176
                     /gene="trpE"
                     /locus_tag="VKL21_02130"
     CDS             1653..3176
                     /gene="trpE"
                     /locus_tag="VKL21_02130"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HMB44598.1"
                     /translation="MKFDIGEAEFIRLAKDRPSIIQLKAKVKAQCSPLELYSTLDKNC
                     AYLLESVEKEKKHARFSFVGAEPDAIITIKDRKISLDYQQRTNLIGMIESSLSNICDP
                     EIGKTFMLKAGFDTMDALRSAFSAPGVKFKGIGFDRQTFLGGAIGYCAYDMVYDCWLD
                     IPPKDSIIPDAQFALTTRTVVFDHLTDDTFIVITPFILEGDNALEIYHKALDDASKIA
                     SVIGSARPFLPEKNNPLEIRCNMHKDEYEEIVRKAKKHIIDGDIFQVVLSRRYEVTTE
                     QKPLELYSALRKVNPSPYMYLFEFNGTAIIGASPETLMSVHNRKLIINPIAGTCMRGK
                     TPEEDEKYAKEMLDSEKEKAEHVMLVDLGRNDVRMVSKGGTVKVDDFMSVIKYSHVQH
                     IESTVSGQLRDECDQFDATRAIFPAGTLSGAPKIRAMEIIHDLEKDARGIYGGGVGYY
                     SWNGDADFAIVIRTIIKNGNLAIVQAGAGIVADSDPEYEYNETERKMAAMIKAIGGL"
     gene            3173..3760
                     /locus_tag="VKL21_02135"
     CDS             3173..3760
                     /locus_tag="VKL21_02135"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMB44599.1"
                     /translation="MKILFVNNKDSFVWNLVDYVSMFEPDTVVVPNTISLSEIEDHKP
                     DAIVISPGPGHPTNHRDIGNCLEIIRGSTIPLLGVCLGHQAIAVAFGGEVSHSPSGPL
                     HGKISPIDHNGNGIFRGLPNPLVGGRYHSLAVTVLPHDLEVTARSEDGIIMGIKHKER
                     PIFGLQFHPESVLTPHGLKIVGNFVKITEDFIIQH"
     gene            3789..3863
                     /locus_tag="VKL21_02140"
     tRNA            3789..3863
                     /locus_tag="VKL21_02140"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3824..3826,aa:Met,seq:cat)
     gene            3989..4948
                     /locus_tag="VKL21_02145"
     CDS             3989..4948
                     /locus_tag="VKL21_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB44600.1"
                     /translation="MKETERKQSESQIVDVKDFAKSFVDTIHEPFLLLDKDKKITYVN
                     RGFCDTFKVTVDETIGKVIYNIGKWQWDIPRLRMALDDLLQNEKFFNGFVVEHDFPTI
                     GRKVMMLNARIIEGKHEPLILLGIVDFTGIRKRENKLKEYDARFKRSFETAHDGLLLI
                     EYNTGQIVNSNPAISSMLGYSAEDFKGKSLKDVGLIELEFQDLISNLKEFGFIHFDYF
                     PAKTKEGKTIDTEIYLVDRTKLIQCNVRDITERKKKENELHIYHENLAEKVKEKNMEL
                     NAKIQDLETFNKAFVGRELRMIELKKIIEELEKEVASLKKSES"
     gene            5108..>5449
                     /locus_tag="VKL21_02150"
     CDS             5108..>5449
                     /locus_tag="VKL21_02150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HMB44601.1"
                     /translation="MNFIHASDFHLGYAQYGLEERFRDYARAFHSVIKYAIDNRAEFI
                     LISGDLFHKRNINAPTYLQAYKVLLELKEKSPSTQVFAIEGNHDLAHHQDGNSWLEIL
                     NSQGLLRLIRLK"
BASE COUNT         1708 a         1097 c         1275 g         1369 t
ORIGIN      
        1 cagagcctca caacagaaga ggccgagctt gcgatatcta agatatttac cgatgccact
       61 gacatccaga tatcagcgtt tttaacggcc atgaaaatga aaggtgaaac gccttccgag
      121 atcgcaggac ttgccatggg catgaagaag gccgcaaata tcattgatcc tcaagtcaaa
      181 gggacactta tcgacacgtg cggtactggc ggcgactcaa caggtactat taatgtgagc
      241 accggagcag cgcttgttgc tgcgggagca ggcgttcctg tcgcaaaaca cgggaactat
      301 tcgatcactt caaaatcagg aagcgctgat gtgctaagag aacttggtgt aaagatagac
      361 gcacctccgg aacaggttga aaaaaccatc gaaaaagttg gcatagggtt catgcttgcc
      421 ccggtatttc atccttccat gaagcgtgtg gcgcagataa ggcgtgacct gggatttcgg
      481 acagtgttca atatcctggg accgctaaca aatcccgcga acgcaaaagc gcaggtcatc
      541 ggcgtctttg atagttcctt atgcgagccg attgcaaatg tccttcatat tttaggcaca
      601 aaaagggcgc ttgtagtcca tggcagcggc atggatgaga tctcgacaac aggtgaaaca
      661 caaattgtag aattaaacgg tggaaagatc acaaaataca ctttaacacc ggaaaaactc
      721 ggtataaaac gagccgcaaa tggtgatatc gctggcggca cccccgaaga gaatgcaagg
      781 gatattgtgg aagcgctaaa aggcaagaaa gggccaaaac gggacatcat tgccgtcaat
      841 gcgggcgcag cgattcttgt gagcggaaaa gcggaaaatc ttgcagacgg gataaatctt
      901 gcaaaccagg caatagacag cggcgcagcc atgagtaagc tgaaagagct tgtaaacgca
      961 gcaggggaac ctgaaaagct tgcgcggttc ttataaagtg ggcgttgaca gatgaaagta
     1021 aaaatctgcg gtataaagag tgaacgcgac cttgccatgg caataaaagc cggagcggat
     1081 gctgcaggat ttattaccga agttccggtt gattctccaa ggaagatcac acttaccgag
     1141 gcatcgaggt taattgctca tgtccctgtt tttgtgacct cagtgcttgt catcatgcca
     1201 aaaaacgcct atgaagctat cgaaatgata aatgcggcaa ggccgactgc ggtccagatc
     1261 cacaattcac tgccaattaa tgaacttaaa gaaatcaggc aaaccggtgt aaaacttatt
     1321 aaaacaattc cagtgccgat acatgctgat acccggatgc ttctggatat tgtaaaaggt
     1381 ctttcaggaa tagttgatgc agtgctcctt gataccgcgt tagacggaaa aacaggggga
     1441 actggtgttg tgcataactg ggaattgagt tcgaatattg ttcgcaaagc gggcctgcct
     1501 gtgatattag caggcggatt gaatcccacg aacgtaatgg atgcagtaaa gtgcgttcgc
     1561 ccatatgctg ttgatacagc atctggtgtg gagaccgaag gaaaaaaaga tgaaaacaaa
     1621 ttgatcgatt ttatcaagaa tgcgaggatg ctgtgaaatt tgatattggt gaagcggaat
     1681 tcattcgcct ggcaaaagac aggccttcga tcatacagct taaagcaaaa gtgaaagcgc
     1741 agtgttctcc tctggaacta tattccacac ttgataaaaa ttgtgcatat cttcttgaat
     1801 cagtcgagaa agagaaaaaa catgcgcggt tttccttcgt tggagcagaa ccggatgcta
     1861 ttattacgat caaggaccgg aagatatcgc ttgattatca gcaaaggaca aatctgatag
     1921 gaatgatcga atcatccctg tcaaatatat gtgatcctga aattggaaaa accttcatgt
     1981 taaaagcagg atttgataca atggatgccc tgagatctgc tttttccgca cctggagtca
     2041 aatttaaagg tatcggtttt gacaggcaaa ccttcctggg aggagctatt ggttattgcg
     2101 cttatgatat ggtgtatgac tgctggctgg atatccctcc aaaagacagc ataatacctg
     2161 atgcgcagtt cgcgcttaca acgagaaccg ttgtttttga tcatctgact gatgacacat
     2221 ttattgtcat aaccccattt atccttgaag gggataatgc cctggagata tatcataaag
     2281 ctctggatga tgccagtaaa attgcttcag ttataggatc tgcccgacct ttcctaccgg
     2341 aaaaaaacaa ccccctagaa atcagatgca atatgcataa ggatgaatac gaagaaattg
     2401 tccggaaagc aaaaaaacac ataattgacg gggacatttt ccaggtggta ctttcgcggc
     2461 gctatgaagt tacaacggag caaaaacctc ttgaattata ttcagcgctt cgaaaagtaa
     2521 acccaagccc ttacatgtac ctgtttgagt ttaatggaac cgccataatc ggagcgagcc
     2581 ctgaaacttt aatgtcggta cataacagga aactcataat caacccgata gcaggcacct
     2641 gcatgcgcgg aaagacccct gaagaagatg aaaaatatgc aaaagagatg cttgatagcg
     2701 agaaagaaaa agcagagcat gtcatgcttg ttgaccttgg gagaaatgat gttcggatgg
     2761 tttcaaaagg cgggacagta aaagttgatg atttcatgtc cgtgatcaaa tattcccatg
     2821 tccagcatat cgaaagcaca gtgtccggac aacttcgtga tgaatgcgac cagttcgatg
     2881 ccacacgcgc catattcccg gccgggaccc tgtcaggcgc gcccaagatc cgcgctatgg
     2941 aaataataca tgaccttgag aaagacgccc gcggtatata cggcggaggt gtgggctatt
     3001 actcatggaa cggggatgct gattttgcta ttgtgataag gacgataatc aagaacggaa
     3061 atctggcaat agtccaggcc ggcgccggaa tagtagcaga ttccgatccc gaatatgagt
     3121 acaatgaaac tgaacgaaag atggccgcca tgataaaagc gataggagga ttgtgaaaat
     3181 cctgttcgtg aacaataaag attcatttgt gtggaatctt gtggattatg tgtcaatgtt
     3241 cgaacccgat acggtcgtcg ttcccaatac tatttcatta agcgaaatag aagaccacaa
     3301 acctgatgcc attgtgatct ctcctggccc cggtcaccca acaaatcaca gggatatcgg
     3361 aaattgtctt gaaattatca ggggatcaac cattccgctg cttggcgtgt gtcttggcca
     3421 ccaggcgata gccgttgctt tcggcggcga ggtaagtcat tctccttcgg gtccgctgca
     3481 tgggaaaata agtcctatcg atcataatgg caatggaata ttccggggat tgcccaatcc
     3541 gcttgtcggg ggtcggtacc attcacttgc agtaacagta ttgccgcatg atcttgaggt
     3601 gacagcgcgt tcggaagatg ggatcatcat gggaataaag cataaagaaa ggccgatttt
     3661 cgggcttcag ttccatcccg aatctgtgct gacgccgcat ggtttgaaga tagtggggaa
     3721 tttcgtgaag attaccgaag actttatcat tcagcattaa ctataagggg atactatttt
     3781 tacctcaaag cggggtaggg tagtcaggat atcccgacgg gctcataacc cgtagatcaa
     3841 tggttcgaat ccattccccg ctatacaaga atcttgtgag tcagcgtaaa ttattctatt
     3901 tattattatt gttcttaacc gatgaaaaaa tttaaatatc ttcatgacta tataggtttt
     3961 aatttggatg cttgggggta ccaaataagt gaaagaaact gaaagaaaac agtctgaaag
     4021 tcaaatagtt gatgtcaagg actttgcaaa aagctttgta gatacaattc atgaaccttt
     4081 tctgctcctt gataaagaca agaagattac atatgtcaac agaggcttct gtgatacttt
     4141 caaagtgaca gttgatgaaa caataggaaa agtaatttac aatattggaa aatggcaatg
     4201 ggatatccct agacttcgga tggcgctcga tgatcttctt caaaacgaaa agttcttcaa
     4261 tggcttcgta gtggagcatg atttcccaac aattggtcgc aaagtaatga tgctaaatgc
     4321 ccggattatc gaaggaaaac atgagccatt gattcttctg ggcattgtag atttcacagg
     4381 tatcaggaaa agggaaaata agttgaaaga gtatgacgcc cgtttcaaac gttcctttga
     4441 gaccgcacat gacggactgt tgctcatcga atataatacc ggacaaatag ttaattctaa
     4501 tccggccatc agcagtatgc tgggttattc tgcggaagat tttaagggaa aatctctcaa
     4561 ggatgtgggt ctaattgagt tggaatttca agatcttatt tcgaatttga aagaatttgg
     4621 attcatccat tttgattatt tcccggcaaa aaccaaagaa ggaaaaacta ttgatactga
     4681 gatttattta gttgaccgaa ccaaattgat tcaatgcaat gtgcgcgaca tcacagagcg
     4741 aaagaaaaag gagaatgaat tgcacatata ccacgaaaat cttgcagaaa aggttaaaga
     4801 gaaaaacatg gaacttaatg ccaagattca ggaccttgag acttttaaca aggcatttgt
     4861 agggcgtgaa ttgcgcatga tagaactaaa aaaaataatt gaggaacttg aaaaggaagt
     4921 tgcgtctttg aaaaaatcgg agtcatgagt tgaaaataga tattattttc aattattgca
     4981 taaatcatat ctcacttcgt atgaaaaacg aaatttttca tacagtatct atgtaccaat
     5041 cggttcatga atagtttcac accgctccca caatcatttt taaccttaaa aatcatctaa
     5101 atcaactatg aacttcatcc atgccagcga tttccacctg ggctatgcac aatatggact
     5161 cgaagaacgc ttccgcgact atgcccgcgc cttccattct gtaataaaat acgctattga
     5221 caacagggcg gagttcattc tcatatcagg cgaccttttc cacaagcgta atattaatgc
     5281 gcctacctat ttgcaggcat ataaagttct tttggaactt aaagaaaaaa gtccatcaac
     5341 acaggttttt gctatcgagg gaaaccacga tcttgcccat caccaggacg gcaatagctg
     5401 gcttgagata ttgaattcgc agggcctgct gaggctcata cggctaaaa
//
LOCUS       DATGBN010000098         5619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_318304, whole genome shotgun sequence.
ACCESSION   DATGBN010000098 DATGBN010000000
VERSION     DATGBN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318304"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VKL21_02155"
     CDS             complement(<1..495)
                     /locus_tag="VKL21_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44602.1"
                     /translation="MTNMCWRKTVALLKLCNTPATIYSPFPLFAISLFLISRKELSIY
                     DLPVLFAGIVVSWLLAFSSNLWNHCNDLKEDKAGGKKTILTEDVSMQKKALFIAVLLY
                     ACSMLIVYYLSNEFKRPIYLYSLICVIATWWYSDNLILKKVIGFRLKDHYLGELIAYS
                     IAMPM"
     gene            835..3936
                     /locus_tag="VKL21_02160"
     CDS             835..3936
                     /locus_tag="VKL21_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB44603.1"
                     /translation="MEKNNTQNLQQTVRFSETLLCASADGILITDSAKNIIFVNDTFC
                     KFFSSNRDYVIQTNIFVWLEKLGSDAQVVWKRMEDNILEHGIARGIEFEMKAGEGMRY
                     FTVNSSLLEKGDIQEAGVIISIWRDDTQSKMITKLNIALKSQINERKQSEDFAFRRNR
                     QLRLLSSAGQKINTVLEIPAIMQNLVASAIEITDATSGSAGMVVNGKMVFSKYYEHDK
                     LIQIDYSFEKGYGVPGMVMETRSPYFSNDAEHDPYVIQEIRKTLGFTNLANVPILDRK
                     GDILGSFQVHNPKGRRPFDQLDIEMLQGLASSAAVAIENARMMDEIRRSEEDKKILSS
                     ILDKSLNEIYMFDAQTLRFTYVNSGACKNLNYSQEALRSMTPLDLKPEFDKESFHKLI
                     EPLMQHEKNKIMFYTSHRRSDGSLYPVEVHLQLIEKPSKVFLSMITDITERSRMEKDL
                     KLSEEKYRTLYETMAQGVVYHDSTGEIISVNPAAQKILGLSINHLLGRTSIDPAWKAI
                     HEDGLSFPEKMHPSMVALRTRKKVSDVIMGVFNPREKQYRWIIVNAVPQSRYAQTKPY
                     QVYSTFIDITNIKQAEEEIQAVNMELMTMNRLITAATGILDIDKILETVLDESMKIVG
                     FEGGSICLLNPDETLQLTVHRGLSEAMMLDLTANKVKVGECLCGESARVMKPLILPDP
                     GAVEKFNTREASFEKNIRYHAAFPIVTSGKCVGVLCVFTRTDKKLSGKGQKFLETITA
                     QVALSIDNARLYQEGKDYVGKLEKKVAQRTFDLQENQMSLLNLVDDMNRATDDLESAN
                     KRLLELDRMKSMFIASTSHELRTPLNSIIGFTSILLEGWSGELTPEQREQVRIVNTSG
                     KHLLSLINDVIDISKIEAGRIEKHVSKFNLKEVIDEVVSTLKPDLNGRGLLLNVEAAD
                     LYLNTDRKRLLQCLMNLLSNAVKFTEKGEIKVGAKSINNEIEISVEDTGIGMKKEDIP
                     KIFTAFVRLDSPLTNKTSGTGLGLYLTQKLAKEVLMGTIAVESEWGKGSIFSMRIPQE
                     VKI"
     gene            3933..4298
                     /locus_tag="VKL21_02165"
     CDS             3933..4298
                     /locus_tag="VKL21_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB44604.1"
                     /translation="MKVLVIEDNQQNMKLISFVLKKFGHEVIEAFCGLEGVEKAINLQ
                     PELILMDIQLPDIDGLEATRRIRNTLLKIPIIAITSYAMAGDREKVMEAGCNGYFEKP
                     INPLAIMKDIDKVLRGRKP"
     gene            4295..>5619
                     /locus_tag="VKL21_02170"
     CDS             4295..>5619
                     /locus_tag="VKL21_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB44605.1"
                     /translation="MKVMIVDDNKFDRMMLSKMLAYNKYDAVEAGNGIEALKLISKEK
                     PDIVVSDIMMPEMDGFSLLREIRKSKSAKELPFVFYSASYVNEKDRELANALGVSRFI
                     IKPVEPKDLIKEITQILLDFSNGKLEIIEPTLKDDEYLEQYSRRIFKKLQEKVIQLEQ
                     EIDERKRIEEELLKSEAQFRATFEEAPIGMARLDIEGRNLETNHILQQMLGFAGEELK
                     GKVFTEFTHPDDLELDMGEFMKLISGKKDHYMIKKRYIRKKGSVFWARMTVSLVRDAN
                     GAPQFSISMVEDITERKEAEEKLQKSEEKYRTLIENIQDGVFVIQDGEIQYINEFFSR
                     LAGYEVEEVIGKDFVEFIAPEDRIRIADYYIRWEGGEKVPRENEFNIIHKDKKTRITV
                     NANIGFINYRGKVASMGTLKDITERKRAQEDLRIKAQLLDSASDFIYVH"
BASE COUNT         1861 a         1060 c         1264 g         1434 t
ORIGIN      
        1 catcggcatt gcgatgctat aagcaatcaa ttcacccaaa taatggtctt tcaatctgaa
       61 accaatcact tttttaagga tcaaattatc tgaataccac catgtagcaa taacgcagat
      121 taaagaataa agataaatcg gtcgtttaaa ttcattagat aaataataaa caatgagcat
      181 agagcaggca taaagcagga ctgcaataaa aagagctttt ttttgcatcg aaacgtcctc
      241 agttaagatg gtttttttac cccctgcttt atcttccttt agatcattgc aatggttcca
      301 taaatttgaa gaaaaggcca gaagccatga aacaactatc ccggcaaaaa gaacgggaag
      361 gtcataaatt gacaactctt tacgcgatat taaaaaaagc gaaatggcaa agagaggaaa
      421 tggggagtat attgtggctg gagtgttgca caacttcagt aatgcaacag tttttctcca
      481 acacatatta gtcaatttag aaggcccaca gacgtgtttt cttaaacatt tcttctgcta
      541 ctttttcttc ttcatctttc ttctggctga tttttttttc ggtcttcatt ttcatcttct
      601 ccttaattat tgcccttgaa gggcaatcta taatcataac tcttcgattt atatatatgt
      661 ccatgaaaaa aaatgattat cgcaataata attattattg aatatttttg atttttaatt
      721 acgattaaat acgaatttat atcattataa ccacaaccat tatacaaacg aagtaaatat
      781 ataaggtact gtcataggaa taaaaacggg aggaaggaag aattaatgtg ggtgatggaa
      841 aaaaataaca cacagaattt acagcagact gtaagatttt ccgagacgct tctgtgcgca
      901 tcggctgatg gcatactgat tactgattcg gccaagaaca tcatttttgt gaatgataca
      961 ttctgtaaat tcttttccag taacagggat tatgtgatcc aaaccaacat tttcgtatgg
     1021 cttgaaaagc tgggttccga tgcccaggtt gtctggaaaa gaatggaaga caatatcctg
     1081 gaacatggca tagcaagagg tattgagttt gagatgaagg ccggggaggg gatgagatat
     1141 ttcaccgtca attcttctct tcttgaaaag ggcgatattc aagaagcagg tgtcattatc
     1201 agtatctggc gtgatgacac acagagtaaa atgataacca aattgaatat tgcattaaaa
     1261 agccagatca acgaacgcaa gcaatctgag gatttcgcat tcaggagaaa caggcagctt
     1321 cgtctccttt caagcgccgg acagaagata aatactgtgc ttgaaattcc ggccatcatg
     1381 caaaatcttg tagcttcggc aatagaaatc acagacgcaa catccggaag tgcgggtatg
     1441 gtcgtcaacg gaaaaatggt gttttcgaaa tattatgaac acgataaact tattcagata
     1501 gattattctt ttgagaaggg ttacggtgtc ccgggaatgg taatggaaac aagatcacct
     1561 tatttttcaa atgatgcgga acatgatcct tatgttatcc aggaaatccg caaaacactc
     1621 gggtttacta atcttgccaa tgttcccata ctggacagga aaggtgatat cctgggctcc
     1681 ttccaggtac ataacccaaa gggacgccgc ccgttcgacc aactggacat cgaaatgctg
     1741 cagggactgg cttccagtgc ggcagtggca attgaaaatg cccgtatgat ggatgagata
     1801 aggaggagtg aagaagataa aaagatcctc tcttccattc ttgataaaag ccttaatgaa
     1861 atctatatgt tcgatgcgca aaccctcagg ttcacgtatg taaattcagg ggcatgcaag
     1921 aacctgaatt attctcagga agcgctaaga tcgatgaccc ctcttgacct caaaccggaa
     1981 ttcgataaag aatcattcca taaattaata gagcctctca tgcagcatga aaaaaacaaa
     2041 atcatgtttt acacgagcca ccgccggtct gacggcagtc tctatcctgt tgaagtccat
     2101 ttgcaattga tagagaagcc ctcaaaggta ttcctttcca tgatcacaga tatcacagaa
     2161 cgcagccgta tggaaaaaga tctaaagtta tctgaggaga aatatcgcac tctctatgaa
     2221 accatggcgc agggggtggt ctaccatgat tccactggag aaataatttc cgtaaatccg
     2281 gcagcgcaaa agatactggg gttatctatc aaccatttgc tgggacggac atccatagat
     2341 ccggcgtgga aggccataca tgaagatgga ttgagtttcc ccgaaaagat gcatccttct
     2401 atggtcgctt tacgaacgcg caagaaagta agcgatgtta tcatgggagt attcaatccc
     2461 agggaaaaac aatacaggtg gataatagtt aacgcagtcc ctcaatccag atatgcgcag
     2521 acaaagccat accaggtata tagcacattt attgatataa caaatatcaa acaggcagag
     2581 gaagagatac aggcggtaaa tatggaactg atgacaatga atcggttaat taccgcagca
     2641 acaggaattc tggatattga caagatcctt gaaaccgtac ttgatgaatc aatgaaaatc
     2701 gttggatttg aggggggttc gatctgtctt ttgaatcccg atgaaacatt gcagcttacc
     2761 gtgcacaggg gattatcaga ggcaatgatg cttgacctca ctgctaataa ggtgaaagta
     2821 ggagaatgcc tttgcgggga gtcggcgcgt gttatgaagc cccttatcct tcccgaccct
     2881 ggagcggttg aaaaattcaa cacgcgtgag gcatcttttg agaaaaatat acgataccat
     2941 gcagcttttc ccattgtgac ctcaggtaag tgcgtgggtg tcctgtgcgt atttacccgt
     3001 actgataaaa aattatcagg gaagggtcag aaatttcttg aaaccattac cgcgcaggtc
     3061 gcgctttcaa tcgataacgc acggctttac caggaaggaa aagactatgt cggaaagctc
     3121 gagaagaaag tggcgcaacg gacatttgac ctccaggaaa accagatgtc cctcctcaat
     3181 cttgtggatg acatgaaccg cgcgacagat gaccttgaaa gcgcgaacaa acgtcttctt
     3241 gaacttgacc gcatgaaatc catgttcata gccagcacaa gccatgaact caggactcct
     3301 ctgaattcca taatcggatt tacaagcata ctccttgaag gctggtcggg agaactcaca
     3361 cctgaacaaa gagaacaggt ccggatagtc aatacttcag gaaagcatct tctttcattg
     3421 ataaacgatg taatagacat ttccaagata gaagcaggaa ggattgagaa acatgtctcg
     3481 aagttcaatt tgaaagaagt gatcgatgaa gtagtttcta ccttgaaacc cgatctcaat
     3541 ggaagggggc ttttgctgaa tgttgaagca gccgacctgt atttaaacac tgaccgcaag
     3601 cgacttctcc agtgtttaat gaatcttctc tcaaacgctg tgaagttcac agaaaaaggg
     3661 gagattaaag tgggggcaaa atctattaac aatgaaatag aaatatcagt tgaagatacg
     3721 gggattggga tgaagaaaga agatattccg aagatattta ctgcatttgt gaggcttgat
     3781 tcaccactca ccaacaaaac ctccggaaca ggacttggtt tatatcttac ccaaaagctt
     3841 gcaaaagaag tccttatggg tacgatagca gtggaaagcg agtggggaaa gggcagtatt
     3901 ttttccatgc gaataccgca agaggtcaaa atatgaaggt actggtaatt gaagacaatc
     3961 aacagaatat gaagttgatt agttttgtcc tgaaaaagtt cggacatgaa gttattgagg
     4021 ctttttgcgg attggaggga gtagaaaaag caataaattt acagcctgaa cttatcttga
     4081 tggatataca gcttccggat atcgatggtc ttgaggccac gcgcaggatc cgtaacaccc
     4141 tgttgaagat ccccattatc gctattacat cttatgcgat ggctggagac cgcgaaaaag
     4201 taatggaggc gggatgcaat ggatattttg aaaaaccaat aaatcctctt gcaattatga
     4261 aagacattga taaagtcctc aggggaagaa aaccatgaaa gtcatgatag tggatgataa
     4321 caagtttgac aggatgatgc tttcaaaaat gttagcatac aacaaatatg atgcagttga
     4381 agccgggaat ggcatagaag cattgaaact gatatcaaag gaaaaacctg atattgtggt
     4441 ttctgatatc atgatgcctg aaatggatgg tttctcgctt cttcgtgaaa tcaggaaaag
     4501 caaatctgca aaagaattgc ctttcgtatt ctacagcgca tcttatgtga atgaaaaaga
     4561 ccgggaactg gcgaatgcac tcggagtctc tcgattcata atcaaacctg ttgaacccaa
     4621 agatcttata aaggaaataa cacagatact tctggatttt tcaaatggta aacttgaaat
     4681 catagagccc actttgaagg atgatgagta tcttgaacaa tacagccgcc gcattttcaa
     4741 gaagcttcag gagaaagtaa tccagcttga gcaagaaatt gatgagcgaa agcggataga
     4801 agaagaactt ctaaaaagcg aggcacaatt cagggctact tttgaagagg cccccatcgg
     4861 tatggcacga ctggatatag aagggcgaaa cctggaaaca aatcatatac tgcagcagat
     4921 gcttggattt gcaggcgaag aacttaaagg taaagtgttc acagagttca cacatcctga
     4981 tgacctggaa ctcgacatgg gcgagttcat gaaattgatc tcagggaaga aagaccatta
     5041 catgataaag aaacgctata tccggaaaaa aggctcggta ttctgggccc ggatgacagt
     5101 ttccctggtc cgggatgcaa atggtgctcc gcaattttca atcagtatgg tggaggatat
     5161 cacagaacgt aaggaagctg aggaaaagct ccagaagtcc gaagaaaaat accggacttt
     5221 gatcgaaaat atccaggacg gtgttttcgt tatccaggat ggtgagattc aatatataaa
     5281 tgagtttttc tccaggctcg cagggtacga agtagaagag gtaattggaa aggatttcgt
     5341 ggaattcata gcgccagagg acagaataag gatcgctgat tattatattc gctgggaagg
     5401 aggcgaaaaa gtcccacgcg aaaatgaatt caacataata cataaagaca aaaaaacaag
     5461 gattaccgtc aatgcgaaca tagggttcat aaattatcgg ggaaaggtag cgagcatggg
     5521 aacgctcaag gatataaccg agcgaaagcg cgcccaggaa gatttgagga taaaagcgca
     5581 gcttcttgat agcgcgtcag actttattta tgttcatga
//
LOCUS       DATGBN010000099         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_201738, whole genome shotgun sequence.
ACCESSION   DATGBN010000099 DATGBN010000000
VERSION     DATGBN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201738"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /gene="thsA"
                     /locus_tag="VKL21_02175"
     CDS             <1..1213
                     /gene="thsA"
                     /locus_tag="VKL21_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HMB44606.1"
                     /translation="IDASDDVLENIAKTALTGKSADLALDSLVKLCVETAKSIKEGGK
                     VNIKENINIIHQRGGSIKDTKFVHGLVIDKARAHKNMPRRIENAKIAVLDTGIEVEKT
                     QVKSKLKITSPELISEARLEESKIVEERIEALVKSGANVIFTEKGIDDIGMHYLAKKG
                     IFAVRRCKEEEIKKIVKATGARVVSKVDGVEAEDLGKAALVEERGVGNYKMVYVEGCE
                     NPKAVSILIYSGAEHVADEVERALDDALRVVGVVIEDGKIVAGGGSPEIEIATRLRKY
                     ATTHGGREQLAIMAYAQALEEIPKAIAENAGLDAIDIIVELRNKHDKGVKNVGLNVFS
                     GKAEDMLKLGVIEPLRVKTQEIKSATDAAVMILRINDILMAKETGMMDVKPEHTADFY
                     DGIQAPDVGED"
     gene            1325..1927
                     /locus_tag="VKL21_02180"
     CDS             1325..1927
                     /locus_tag="VKL21_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMB44607.1"
                     /translation="MKIRENRKKMDAGKIVPEASETEEEITETTEEKLCKDLDIARKQ
                     LDEEKDMYLRLNAEFDNLRKRTLKEREEFIKYANEKLIQEFIDVFESLERGIENARKT
                     DNKDKLIEGMELVYKQFKVVLERNGLVPIKALGEKFDHSRHEAMMQTLTDDHEEDIIL
                     QEFAKGYMLNGKILRYSKVRVSKKKGSEEESDNEIENEVK"
     gene            1927..>2025
                     /locus_tag="VKL21_02185"
     CDS             1927..>2025
                     /locus_tag="VKL21_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HMB44608.1"
                     /translation="MAKIIGIDLGTSNSAAAVMQGNRPVIIPSAEGV"
BASE COUNT          728 a          301 c          521 g          475 t
ORIGIN      
        1 cattgatgct tctgatgatg ttcttgaaaa tatcgcaaag acagccctta ccggaaaaag
       61 cgcggatttg gctcttgatt cacttgtcaa gttatgcgtt gagaccgcca agtcgataaa
      121 ggaaggtgga aaagtaaaca taaaggaaaa catcaatatc atccaccagc ggggaggaag
      181 cataaaagat acaaaattcg ttcatgggct tgttattgat aaggcaaggg cgcataagaa
      241 tatgccaaga cggatcgaga atgcaaagat agcagtactt gatacgggca tagaggttga
      301 aaagacacag gtgaaatcta agttaaagat aacttctcct gaactcattt ccgaggcaag
      361 gcttgaggaa agcaagattg ttgaggagag aatagaggcg ctggtaaaaa gtggcgcgaa
      421 tgtaattttt actgaaaaag gtatagatga tatcggtatg cattatcttg caaagaaagg
      481 tatttttgca gtgcgaagat gcaaggaaga ggaaatcaag aaaattgtaa aagccacagg
      541 agcgcgcgtt gtctcaaagg tggatggcgt tgaagctgag gaccttggga aagcagcgct
      601 tgtagaagag cggggtgtgg gtaattataa gatggtctat gtggaaggat gcgagaatcc
      661 aaaagcagta tccatactga tctacagcgg cgcagagcat gttgcagatg aagtggagcg
      721 agcccttgat gatgcactgc gtgttgtggg cgttgttata gaagacggga agatcgtagc
      781 agggggcggt tcaccggaaa tcgagatcgc tacacgcctt cgcaaatatg cgacaacgca
      841 tgggggcagg gagcaattag cgataatggc atacgcacaa gcgcttgagg aaatacccaa
      901 agcaatagca gaaaacgcag gtctggatgc aatcgatatt attgttgaac tcaggaataa
      961 acatgataaa ggtgtaaaga atgtcggatt gaatgttttt tcgggaaagg ccgaagatat
     1021 gcttaaacta ggtgttatcg aaccgcttcg cgttaagacc caggagatca aatcagccac
     1081 tgatgccgct gtaatgatct tgcggatcaa tgatatcctt atggcaaaag aaactggcat
     1141 gatggatgtc aaacccgaac atacggcaga tttttacgat ggtatccagg ccccagatgt
     1201 gggagaagac taatattaag tagaatgaag tttatgataa tataatacct tatgggaatg
     1261 tggtgttgtg ttgcatgaat ttaatattta attttttcaa aattctttat ggagtagttt
     1321 ataattgaaa ataagggaaa acagaaaaaa aatggatgcc gggaaaatag taccagaggc
     1381 ctctgaaacc gaagaggaga taactgaaac aacagaggaa aagctctgta aggatttaga
     1441 tatcgcaaga aaacaactcg atgaagaaaa agacatgtat cttcgtctga acgcagaatt
     1501 cgataatctt cgaaaaagaa cattaaaaga gcgggaggaa ttcataaaat acgcaaatga
     1561 aaagctcata caggagttta tagatgtatt tgaaagtctt gagcggggaa ttgaaaatgc
     1621 caggaaaacg gataataagg ataaattgat cgaaggcatg gaacttgttt ataaacaatt
     1681 taaagtcgtg ctcgaaagga atggtcttgt ccctattaaa gcgctgggtg agaaattcga
     1741 tcattcaaga cacgaagcaa tgatgcaaac actaacagat gatcacgaag aggatataat
     1801 actccaagaa tttgcaaaag gttatatgtt aaatgggaaa atccttcgtt attctaaagt
     1861 tagagtatcg aaaaagaagg gatcagaaga agaatctgat aatgaaatag aaaatgaggt
     1921 aaaataatgg ctaaaataat tggaatagat ttgggtacaa gcaattcagc agctgcagta
     1981 atgcagggga atagaccagt aataatacca agtgcagagg gtgta
//
LOCUS       DATGBN010000100         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_434806, whole genome shotgun sequence.
ACCESSION   DATGBN010000100 DATGBN010000000
VERSION     DATGBN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434806"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VKL21_02190"
     CDS             <1..325
                     /locus_tag="VKL21_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44609.1"
                     /translation="LPLSCAFWYKKCRYSKSCFTQKEVSFKINDTDTLNTSQKFWRKS
                     KISYKVFVHKKIVDKILSEIDEQDRYKIAKGKTCIKGIYMVHYDNPVLANKYKDAFSL
                     DSTNV"
     gene            341..1381
                     /locus_tag="VKL21_02195"
     CDS             341..1381
                     /locus_tag="VKL21_02195"
                     /inference="COORDINATES: protein motif:HMM:NF020476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HMB44610.1"
                     /translation="MIFKGWEKVQIETDERKIIDGIAPIIISASRSTDIPAFHSKWFI
                     HRLNKGYIKWTNPFNRVPQYVSFEKTRVIVFWTKNAAPMLPFLKEIDDKRINYYFQFT
                     LNDYEKEGFEPSVPKLEERIATFKKLSDLIGKEKVIWRFDPLILTDTIGIEELLNKVE
                     KVGNAIHNHTNKLVISFADIEPYAKVKRNLIAAGFKYEDFDKDSMDKISEGIQKINEK
                     WGLEIATCAEISDFDKYNIKHNKCIDDDLMIKLFGNDSLLMDFLYAKKENKVGLAAFG
                     GGSEPEITKDTKLKDKGQRKECGCVVSKDIGQYNTCGHLCVYCYANYSENVVHKNLDG
                     RQIYTGESILNN"
     gene            1867..3030
                     /locus_tag="VKL21_02200"
     CDS             1867..3030
                     /locus_tag="VKL21_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44611.1"
                     /translation="MFDIEEIPALKRAIRTRAHADKKLLDELRDEVHSFACEVRTIKP
                     RSTTAVSLVASDGGNNKLTFDPFLVQLVRVMDSYGKQLCLDAVSPTTDTDELSKNQFN
                     SDGSPRTALGKMMIGLGVEPPTLNRLSYMIPDGRTIRERPEGISPSWVLNYRDLCEWA
                     VLYERICHNSFATDTLIVRDGLLRSPIFRGEFFMTWRKKVEEAIARIYHQDHRQVFLV
                     GVAKHSQVITRYQLAMAIEDIFPPGEPRYVRIPRELEAKSYIRDRYARGPETEGDKRE
                     APKFVAGDMYLVRFGRRSGDPVWAVDILSHQSQKASEIFGYLLSDAIDGFPVPFYPRC
                     LQKAHEHAQVVDFDLDIFQDEIFAAVLDLLPPEKQNIIESFRFKSDLSRGRYG"
     gene            3027..4427
                     /locus_tag="VKL21_02205"
     CDS             3027..4427
                     /locus_tag="VKL21_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HMB44612.1"
                     /translation="MKLFSKEHVVGIFRGFNEGGLEFHAEIILPYRDEFQSAPMHGQF
                     LIVKLETEDEGVLGRITSISSEGRLSSSSGEDYGIRAMLDDRPIPEDLRVQYLKYRVN
                     IRVLGVVRNVGDKFVFAASHRRLPHVGSKVALLSDEVLREVAGHNLDGIDLGFFALGE
                     FIYAGGDTRLKSEPWFKIRNPAIIPRFNVQSLVSRRSFVFARAGFGKSNLNKLLFSSL
                     YKETPTVEKRGGRKVPVGTIIFDPDGEYYWPDDKSRPGLCDVPELEDKIVVFTKKKVS
                     SPFYQSFVAGDIKIDIRRLRPSEVISIALVKEKQDQQNVGKLKGMNDENWRKLVDEIF
                     QNGNAADPILIKELLHLDPGQDAEMVAARANVTTIVKMLHDPSSQMMDMLIYSLKQGK
                     ICIVDVSQMRGKPALILSGLILQKIFDHNQQEFTEAQPETIPTIAVVEEAQSVLGSSG
                     SSGEGPYVSWVKEGRK"
     gene            4515..5174
                     /locus_tag="VKL21_02210"
     CDS             4515..5174
                     /locus_tag="VKL21_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44613.1"
                     /translation="MLSSGDLQALKKANAHFSDDLLSTLLNEPLIGHGTFWSSADGKS
                     YPIPIRILSFEALYKARDPQYNLPAAKTFAQELKTRFKAAVSASLDSIPATQMKVVSG
                     EETQASSNETMDDTIDVMETYISSAIEAFRNDIISIERIKRDGMTWRGIVAILENALP
                     EVIDQRERNRVAYNFARNALNEIFGDGGWDTEKRSKVGGSGIVTWVVIKNKNQKSIEK
                     F"
     gene            complement(5304..>5779)
                     /locus_tag="VKL21_02215"
     CDS             complement(5304..>5779)
                     /locus_tag="VKL21_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMB44614.1"
                     /translation="ERGYEIITDILEVSLKAIEGCLCYEGCPSCIQSPKCGNHNEPLD
                     KHAAIMILHGLLGKSPYVPPKSKPENNREIEWYKKGVEFNNIGKYNDAIECYKKALEI
                     NPKNEKTWNNIGSVYFKLKMYLEALKSYEKAIEIRPSNDMISKNLRKARQVLKRA"
BASE COUNT         1857 a          946 c         1206 g         1770 t
ORIGIN      
        1 tttaccacta agttgtgcat tctggtataa aaaatgtaga tactctaaat cttgttttac
       61 tcaaaaagaa gtatctttta aaattaatga tacagataca ttaaatacta gtcaaaaatt
      121 ttggcgcaaa agtaaaattt catataaggt atttgtccat aaaaaaatag tagataaaat
      181 tttatcagaa attgatgaac aagatcgtta taaaattgcc aaaggcaaga cttgtataaa
      241 aggtatatat atggttcact atgataatcc agttttagct aataaataca aagatgcatt
      301 ttctttagat tctactaacg tttaatacaa aggattaaaa atgatattta aaggatggga
      361 aaaagtacag atagaaacag atgaaaggaa aataatagat gggattgctc caattattat
      421 ttcggcaagt agatcaactg acattccagc atttcattca aaatggttta ttcataggct
      481 taataaaggc tatataaaat ggacgaatcc atttaataga gtccctcaat atgtttcctt
      541 tgaaaaaaca agagtaattg ttttttggac aaaaaatgct gccccaatgt tacctttttt
      601 aaaagaaatt gatgataaga ggattaatta ttatttccaa ttcaccctca atgactatga
      661 aaaagaaggt tttgaaccat cagttccaaa attggaggag agaattgcca cttttaaaaa
      721 attgtcagat ttgattggaa aagaaaaagt gatctggaga ttcgatcctt tgattttgac
      781 cgatacgatt ggtatcgaag agctacttaa taaagttgaa aaagtcggaa atgcaattca
      841 taatcatacg aataaactag tgattagttt tgcagatata gaaccatatg ctaaagttaa
      901 aaggaattta attgctgctg gtttcaagta tgaggatttt gataaagata gcatggataa
      961 aatttcggaa ggtatccaaa aaataaatga aaaatggggt ttggaaattg ccacttgtgc
     1021 tgagatatcg gattttgata agtataatat caaacacaat aaatgtattg acgatgattt
     1081 gatgattaaa ttatttggaa atgactcttt attaatggat tttttatatg ccaaaaaaga
     1141 gaataaagtt ggtttagcag cttttggagg tggttctgaa ccggaaatta caaaagatac
     1201 taaattaaaa gacaaaggac agagaaaaga atgtgggtgc gtggttagta aagatatagg
     1261 acagtataat acatgtggac atttatgtgt ttattgttat gcgaattatt ctgaaaatgt
     1321 agttcataaa aatctggatg ggcgacaaat atatacaggt gaatcaattt taaataactg
     1381 acattatatt ccgaagtaac aataaaatgg aaggcctgtc taccaacatc atgagataag
     1441 tgaaagcgtc gctcactccg ccaagttgca ttcactcagg cacggctgat gccttgcggt
     1501 cattgtcgct gctgtcatgg tgacagtcct gcgtcgggtg ctcagacagt cgccggtgtc
     1561 ggtagcatgt ttctttaagg ctgcactttg tttatgtata tgggggcctg gatacctgaa
     1621 gattacaggg caggaatact ctctttccat gggcagttgg gtagcataga tgtgccctcg
     1681 ctgttctatg acttcattca tcaatgattt gccgctgttg aatactctaa taaggaattg
     1741 aaatatggtc tttttctcac actcttgttt atagccatgt gaaaagtgga atagaatatg
     1801 attaaatttg attgtgcatt cggattttca atattaatca tacaggcaat attggagcaa
     1861 tgaaagatgt ttgatattga agaaatacct gcactgaaaa gagctatcag aacccgggca
     1921 catgcggata agaaattact tgacgaactc agagatgaag ttcattcatt tgcttgtgaa
     1981 gtccgtacta ttaaaccgcg ttccacaacc gctgtttcat tagtggcaag cgatggagga
     2041 aataacaaac ttacattcga tccttttctt gttcaacttg tcagagttat ggattcttat
     2101 ggaaaacaac tatgtcttga tgccgtatcc cctaccacgg atacagatga actcagtaag
     2161 aatcagttca attctgatgg aagccccaga acagctctcg gaaaaatgat gattggcctt
     2221 ggagttgagc caccaaccct aaataggctt agctatatga ttccagatgg caggacaata
     2281 cgtgaaagac cagaggggat ttccccatca tgggtattga attatcgcga tttatgtgaa
     2341 tgggctgtac tgtatgaaag aatttgtcat aattcattcg ctacagacac cctaatcgtt
     2401 agagatggac tccttagaag tccgattttt cgtggcgaat tcttcatgac atggagaaaa
     2461 aaagtagagg aggcgattgc aagaatatat catcaggatc atagacaagt tttcttggta
     2521 ggcgtagcta aacatagcca agtcataaca cgttatcaat tagcgatggc aattgaagat
     2581 atatttcctc ctggtgagcc gcgatatgtt cgcattccca gagaacttga ggcaaaatct
     2641 tatattaggg atagatatgc acgcggtccg gagacggaag gagataaaag agaggcgcca
     2701 aaattcgtag ctggagatat gtatcttgtg agattcggtc gtcgtagcgg tgatccggtt
     2761 tgggctgttg acatattatc tcaccagagt caaaaggcat cggagatttt tggttatctt
     2821 ctttccgatg ctatcgatgg ttttccggtt ccattctatc ctcgttgtct gcagaaagca
     2881 catgaacatg cgcaagttgt tgattttgat cttgatattt ttcaagatga aatcttcgca
     2941 gcagtacttg atcttcttcc cccggagaag caaaatataa ttgagtcttt cagatttaag
     3001 tcagatttat caagagggag gtacggatga aattattttc aaaagaacat gttgtaggaa
     3061 tttttcgtgg attcaatgaa ggtggtctgg aattccatgc tgaaataatt ttaccttatc
     3121 gtgatgaatt ccagagtgca cctatgcatg ggcaattcct tatagttaag cttgaaacag
     3181 aagatgaggg tgtattaggt cgtataacat cgatttcctc cgaaggaaga ctcagctcaa
     3241 gttctggaga agattatggc atccgcgcta tgttggatga ccgcccgatt ccagaagacc
     3301 ttcgagtaca atatcttaaa tatcgggtga atattagagt tttgggagta gtacgtaatg
     3361 ttggtgacaa atttgttttt gctgcttctc atcgtaggct acctcatgta ggaagtaaag
     3421 tagcactact ttcagatgag gtgcttcgtg aagtcgcagg ccataattta gatggaattg
     3481 atttggggtt ctttgcactt ggagagttta tctatgctgg cggagatacg cgtttaaaaa
     3541 gtgagccgtg gtttaaaatt agaaatcctg caattattcc acgttttaat gtccaatccc
     3601 ttgtttcacg aagatcattt gtctttgcaa gggcaggttt tggcaagtca aatctcaata
     3661 aactcctttt tagtagttta tataaagaga ccccaactgt agaaaaaaga ggtggtcgaa
     3721 aagtacctgt gggtacaata attttcgatc cagatggtga atattattgg cccgatgata
     3781 aaagtcgacc aggtttgtgt gatgtaccag aattagaaga taagattgta gtttttacaa
     3841 aaaagaaagt atccagtccc ttttatcagt cttttgtagc tggtgatatc aagattgata
     3901 ttcgacgttt gaggccatca gaagtgattt caattgcttt agtgaaagaa aaacaagatc
     3961 aacagaacgt aggaaagctt aaaggaatga acgatgaaaa ctggcgaaaa ttagtagacg
     4021 agatattcca aaatggcaat gcagctgatc caattcttat taaagaatta ctacatcttg
     4081 atccagggca agatgctgag atggttgcag ctcgggccaa tgtaaccacc atagtaaaaa
     4141 tgcttcatga tccgagtagc caaatgatgg acatgctgat ctattctcta aaacaaggta
     4201 aaatttgtat agttgatgtt tcccaaatgc gtggcaaacc cgcattgatt ctctcaggcc
     4261 taattcttca gaaaattttt gaccataacc agcaagaatt tacagaagca cagcctgaaa
     4321 ccatacctac cattgcagta gttgaggagg cgcaatctgt actaggtagt tctggtagct
     4381 caggtgaagg accatatgtt tcttgggtga aagaaggacg aaaataagat cttggcgcag
     4441 tactcataac tcaacagcca gggagcattt ccagtgaaat tcttagtcag ggagacaatt
     4501 ggtttgtctt tcacttgtta tcctcaggag accttcaagc acttaaaaaa gcaaatgctc
     4561 attttagcga tgatttgtta agtacactcc taaacgaacc tcttatagga catggtactt
     4621 tctggagtag tgctgatgga aagagctatc caattcccat aagaattcta tcatttgaag
     4681 cactttataa agccagagac cctcaatata atcttcctgc cgcaaagaca tttgctcaag
     4741 aattaaaaac acgttttaaa gctgcagttt ctgcaagtct tgattctatt cctgcaacgc
     4801 agatgaaggt cgtatctgga gaagaaacgc aagcttcatc aaacgaaact atggatgata
     4861 ccatagatgt aatggagaca tatatttctt cagctattga ggcttttaga aatgatatca
     4921 tttcaattga acgcataaag agggacggga tgacatggag aggtatagtg gcaattcttg
     4981 agaatgcttt accagaagta atcgatcaac gagaaagaaa tagagttgct tacaattttg
     5041 cacgaaatgc actcaatgaa attttcggag acgggggatg ggatactgag aaacgatcta
     5101 aagtcggtgg ttctggaata gttacatggg ttgtgattaa gaataaaaat caaaaaagta
     5161 tcgagaaatt ttgaaaaagg ctgtttttag gtcaataagt tattaagcca tattacgttt
     5221 ttctgcatat aatgaagtga tcgatatagg gcatttctga tgaaagtcaa aggggtaaga
     5281 tactgaccac gagtgacgta cgctcatgcc ctctttaata cttgcctagc ttttcttaga
     5341 ttttttgaga tcatatcatt tgatggtctt atctcgattg ctttttcata agattttaag
     5401 gcttcaagat acatttttaa tttaaaatat acactaccga tattattcca agtcttttca
     5461 ttttttggat ttatttctag agctttcttg tagcattcga tcgcatcatt atatttacct
     5521 atattattga attcaactcc ttttttgtac cattctattt ccctattatt ctccggcttt
     5581 gacttcggcg gtacataagg agatttacca agtagcccat gaagaatcat tattgctgca
     5641 tgtttatcca gcggttcatt gtggttcccg catttaggcg actggatgca actggggcag
     5701 ccttcgtagc acagacatcc ttctatcgct ttaagtgata cctccagaat atcggttatt
     5761 atttcatatc ccctttcag
//
LOCUS       DATGBN010000101         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_124763, whole genome shotgun sequence.
ACCESSION   DATGBN010000101 DATGBN010000000
VERSION     DATGBN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124763"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..321)
                     /locus_tag="VKL21_02220"
     CDS             complement(109..321)
                     /locus_tag="VKL21_02220"
                     /inference="COORDINATES: protein motif:HMM:NF016319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HMB44615.1"
                     /translation="MTRIRTDPWKPLPIMAQTSEDILRSQGSIYVHSLTMETLMSAIK
                     RGSRSIGFVGPSCNIDAVHKMQNSPY"
     gene            complement(379..1569)
                     /locus_tag="VKL21_02225"
     CDS             complement(379..1569)
                     /locus_tag="VKL21_02225"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMB44616.1"
                     /translation="MTVELKEGVYWVGVVDWNIKQFHGHEYSTHRGTTYNAYLIIDEK
                     IALVDTVWSPYSQEMIENIGKIIDIKKIDYVIANHAEVDHSGGLPELMKLIPDATVVV
                     SEKGRESIPKHYHEDWNFKVVKTGDSISLGKNSLVFVAAPMLHWPDSMFTYLTGKNIL
                     MPNDAFGMHFASSSRFNDEVDEIEVYQEAIKFYANILTPFSDLVIRKIDEFKKLNIPV
                     DIIAPSHGIIWRKDPMQIVQKYYEWAQGKNDGSVVIVYDTMWKATEKMGEAIAEGLEK
                     EGIKYKFFNMAVCDRNDVLTEIFKTKGIIIGSPALNNGLLPTIKPILEDLKGLKFKNK
                     VGAAFGSFGWSGENVKLIEETLEKAKIKILQEGIKFKWQPSKEELEKCVEFGRSFAQK
                     MKAV"
     gene            1695..>1960
                     /locus_tag="VKL21_02230"
     CDS             1695..>1960
                     /locus_tag="VKL21_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HMB44617.1"
                     /translation="MSKNPLPEQDRIQAIDFIKGIDIILMVLFNYSITLDYFNLIQIP
                     SSYLYRYILPISIASIFIFMSGVTAYASYEKHRDKLTKRYFIRG"
BASE COUNT          518 a          430 c          356 g          656 t
ORIGIN      
        1 tgggataccc tttgaagcga ggaaggtttt cagaccttca taattgaaag tgtccataca
       61 gaaaagcccc attttcagga tatttgccct catgaacata tgcagcaatc aatatggcga
      121 attctgcatt ttatgaactg catcgatgtt gcatgatgga ccaacaaacc cgatggaacg
      181 cgagcctctt tttatcgcac tcatcagtgt ctccatggtg agcgaatgca catatatgga
      241 accctgggag cggaggatat cttcactggt ttgcgccatt attggcagtg gtttccacgg
      301 atccgtgcgt atccgtgtca tccgtgcaat ccgtgttcta tcgtatgatt ttttttctta
      361 aacgattacg aatgaattct acacagcctt cattttttgg gcaaatgacc ttccgaattc
      421 cacacatttt tcaagttcct cttttgaggg ctgccatttg aatttgatgc cttcctgaag
      481 gatctttatc tttgcttttt cgagtgtttc ctcaataagc ttgacgtttt caccgctcca
      541 accgaatgat ccgaaagctg cacccacttt attcttgaat ttcaatccct taaggtcttc
      601 aagtatgggc tttatggtgg gcaacaggcc attattcaac gctggtgaac cgataataat
      661 accttttgtc ttaaagatct ctgtaagcac atcattcctg tcgcatactg ccatattgaa
      721 aaatttatat tttataccct ctttttcaag cccctctgct atagcctctc ccattttttc
      781 agtcgctttc cacatggtat cataaacgat cacaacggag ccatcgttct taccctgtgc
      841 ccattcataa tatttctgaa cgatctgcat ggggtctttt ctccatatga tcccgtgact
      901 tggggctatt atatctacgg ggatgttcaa tttcttgaac tcgtctattt tcctgatgac
      961 aagatcgcta aaaggtgtga ggatattggc gtagaatttt attgcttcct ggtacacctc
     1021 tatctcatcc acttcatcat taaaacggga agatgatgca aaatgcatac cgaaagcgtc
     1081 gtttggcatc aatatatttt taccggtaag atatgtgaac atgctgtcgg gccagtgaag
     1141 cataggtgca gccacaaaaa cgaggctgtt cttgcccagg cttatgctat cgccagtttt
     1201 tacaaccttg aagttccaat cttcatgata atgctttgga atactttccc ttcctttttc
     1261 tgaaacaaca acagtcgcat cggggataag cttcatcagt tcgggaagac ctcccgaatg
     1321 gtcaacctct gcatggtttg cgatcacata atctatcttc ttgatatcga taatcttccc
     1381 aatgttctct atcatctcct gtgaatacgg actccagacc gtatccacaa gggcgatttt
     1441 ttcatcaatt ataaggtatg cgttataagt cgtgcctctg tgtgttgaat actcatgccc
     1501 gtgaaactgc ttgatattcc agtccacaac tccaacccag tacactcctt cttttaattc
     1561 cactgtcatt tatatcactc cggttttttg ataatagctg tactggaatt ttagtgttga
     1621 tgatattaaa gactatctga tgcaagagat tatatcagta tcttgcttta aagctgtaat
     1681 atggggatac attaatgtca aaaaatcctc tgccggaaca ggatagaatc caggccattg
     1741 atttcataaa gggtattgat atcatactta tggtattgtt taactattct atcactttag
     1801 attatttcaa tttgatacaa ataccttcaa gttatttata ccgatatatt cttcctattt
     1861 ctatcgcttc tatttttatt ttcatgtcag gtgttacagc ttatgcaagc tatgaaaaac
     1921 acagggataa attaacaaag aggtatttca tacgaggaat
//
LOCUS       DATGBN010000102         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_7786, whole genome shotgun sequence.
ACCESSION   DATGBN010000102 DATGBN010000000
VERSION     DATGBN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7786"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VKL21_02235"
     CDS             complement(<1..253)
                     /locus_tag="VKL21_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44618.1"
                     /translation="MHLLDVQYDQEESTVTCWIKKNDRCLPVSEIYYPEIYIAGNPEL
                     KTLLSTLPGVVATAFDNKKTHLSGTKEPVIAVKVNNTQTI"
     gene            complement(186..491)
                     /locus_tag="VKL21_02240"
     CDS             complement(186..491)
                     /locus_tag="VKL21_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44619.1"
                     /translation="MGRTVPSFRVLLESIVLDLATFRRALRGEDRSAFDIIMNMARKH
                     ASSSTIAPMLDPMDSMFLSILIEQQKEIKNMQEALNASIGCTVRPGGEHSNMLDKKE"
     gene            complement(454..1128)
                     /locus_tag="VKL21_02245"
     CDS             complement(454..1128)
                     /locus_tag="VKL21_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44620.1"
                     /translation="MRASFRPSNPLICGCIDPSGLKNSCITVIEGTSALVPDALFNLC
                     ARTACMEQDVILVDGANSFNPYAISRAVKFMGFEPRSVLSRIHVARAFTEYQMDAIIN
                     DLLEAVNRWDSRLVAVLYLSNLFSTQDGKKLFDPILKNLKETTQSLNIVTVVTSFGGI
                     WWGDRMVAENADRVIRIDEKKTLIRVQDGDNLFEHVSVPPGQTRFIDFGGEMNGQNCA
                     QLQGAA"
     gene            1189..1932
                     /locus_tag="VKL21_02250"
     CDS             1189..1932
                     /locus_tag="VKL21_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMB44621.1"
                     /translation="MKVFPEENGKNEYRCSHTHTFHTHMQPSKLLNMTTVINDYSHYM
                     CGENDVSLREKKKIETRNKIFEVSGRLFKEKGIENTTVDEIVKEAGIGKGTFFNYFPS
                     KTSLLLYFAQQKEELTYSQIKNEALKTVSTREKIKNVLVFVAKTNEKDKELTKIFVFE
                     YMRHYGSSQDERKSHGLSNILYSLIEDGIRKEDLKSTIDAKKAAEIISAIYFHSLVEW
                     LWSKIDYSFSDDISGKIDIVFEGIAGGRT"
     gene            1929..2240
                     /locus_tag="VKL21_02255"
     CDS             1929..2240
                     /locus_tag="VKL21_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HMB44622.1"
                     /translation="MNTSDEKYFMNWDEAFNSTAAQVGGKGYNMARLHRYGFQVSAGG
                     VLISRVYRDFLESNGLSDHINAVASIKAESVLNRRKHENFTDTADESPKNDRWRGCFY
                     L"
     gene            2170..>3367
                     /locus_tag="VKL21_02260"
     CDS             2170..>3367
                     /locus_tag="VKL21_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB44623.1"
                     /translation="MKILLIQPTKAQKTIGGEDVFIYEPLALEYLASGVKEDHEVKIL
                     DMRIDKDLDLQMQKFRPDVVGISAYTVHVNTVKNLFNKIKSINPDIFTVVGGHHATVV
                     PEDFFTPYIDLIVMGEGVFVFKEIISRLVSKRPFDGIAGISYLKGKERVMNPPEPITD
                     LDIFPFPAREFTEKYRKYYYSEWMRPLASIRTSKGCPNRCNFCALWKLTGGKYLKRKP
                     ENIVRELSGIKEKYVFFADDESLVDAGRMKILAELIKEAGIKKRYFLYGRSDTIVKNP
                     DLIKIWKDIGLERVFVGLEFFRDEDLKYINKGSTVENNKEAIKILNSNGIEIYASFIV
                     RPDFTEEDFKEFKEYCGKMEFTFLSFSVLTPLPGTDLYEEVKDRLITHDYDLFDFIHT
                     QLPTKLP"
BASE COUNT         1045 a          637 c          731 g          954 t
ORIGIN      
        1 aaatcgtctg cgtgttattg actttcactg caatgaccgg ctcttttgtc cccgaaagat
       61 gcgttttctt attatcaaat gcagtcgcaa cgactccggg aagtgttgat aaaagggttt
      121 tcagttcagg atttcctgca atataaattt caggataata aatctcactt acaggcaggc
      181 atctgtcatt cttttttatc cagcatgtta ctgtgctctc ctcctggtcg tactgtacat
      241 ccaatagatg catttaatgc ctcctgcatg tttttaatct ctttttgctg ctcgatcagt
      301 attgacagga acatagaatc catagggtca agcatcgggg cgatggttga tgatgacgca
      361 tgttttctgg ccatgttcat tatgatatca aatgcgctcc tgtcctcccc gcgaagcgcc
      421 cttcgaaagg tagcaagatc aaggacgata ctctcaagca gcaccctgaa gctgggcaca
      481 gttctgccca ttcatctcac ccccaaaatc aataaacctt gtctgtcctg gtgggacact
      541 tacgtgctcg aacagattat cgccgtcctg cactcttata agagtctttt tctcatcgat
      601 cctgataacc ctgtcagcat tctctgcaac cattcggtct ccccaccata ttcctccgaa
      661 gcttgtcact acggtcacaa tatttaatga ttgtgtggtt tctttcaggt ttttgagaat
      721 ggggtcaaat aactttttac cgtcctgcgt cgagaaaaga ttagacaggt ataaaaccgc
      781 cacaagccgg gaatcccatc ggtttactgc ttcaagtaag tcatttatga tcgcatccat
      841 ctgatactcg gtgaatgcgc gggcgacatg gatacgcgag agcacacttc ttggttcaaa
      901 acccataaat ttcacagctc ttgaaatagc ataaggattg aagctgttcg ccccgtcaac
      961 aagtatcaca tcctgttcca tgcatgcggt cctggcgcac aggttaaaca gggcatccgg
     1021 tacaagcgcc gatgttcctt caatgaccgt gatgcatgaa ttttttaagc ccgacgggtc
     1081 aatacaaccg catattaatg gatttgatgg tctgaatgag gctctcatgt tttcaccata
     1141 ggaagagaag tctgttttga aggtataata gaacgagcaa agagcggggt gaaagtattc
     1201 ccggaagaga acggaaagaa cgaatacagg tgcagccata cacacacttt ccatacgcac
     1261 atgcagccat cgaaactctt aaatatgact acagtcataa atgactatag tcattatatg
     1321 tgcggagaga acgacgtgtc actgcgtgag aaaaagaaaa tagagacgag aaataagata
     1381 tttgaggtat caggcagatt atttaaagaa aagggtatcg aaaatacaac tgttgatgag
     1441 atcgtgaagg aagcgggaat cggcaagggc acatttttca attattttcc gagtaaaact
     1501 tcacttctcc tttacttcgc acaacagaag gaagaactga cctatagcca gataaaaaat
     1561 gaagcattaa aaaccgtttc gacaagggaa aagataaaaa atgtacttgt ttttgttgca
     1621 aagaccaatg aaaaggacaa agaacttaca aaaatatttg tctttgagta tatgagacat
     1681 tacggatcaa gtcaggatga aagaaaaagt catggcctca gtaacatcct ttacagttta
     1741 attgaagatg gtataagaaa agaagatttg aaaagcacca tagatgcaaa aaaagctgcg
     1801 gaaatcattt cagctatcta ttttcattcc ctggtcgaat ggttatggtc gaagatcgat
     1861 tattcctttt cagatgatat atcaggaaag atcgacattg tctttgaggg tatcgcagga
     1921 gggagaacat gaacacatcg gatgaaaaat atttcatgaa ctgggatgaa gctttcaatt
     1981 ctacggcagc acaggtgggc ggcaagggct acaatatggc caggctgcac aggtatggtt
     2041 ttcaagtttc agcaggcgga gtcttaattt caagggttta ccgggatttt ctggaatcga
     2101 atgggttatc ggatcacatt aatgctgttg cctcaattaa ggcagaatct gtactaaaca
     2161 ggaggaaaca tgaaaatttt actgatacag ccgacgaaag cccaaaaaac gatcggtgga
     2221 gaggatgttt ttatttatga gccgcttgcg cttgaatatc tggcttcagg cgttaaagag
     2281 gatcatgaag taaaaatact tgatatgcgg attgacaaag accttgattt gcaaatgcaa
     2341 aaattcaggc cggatgttgt tgggatcagt gcatatactg ttcatgtgaa tacagtaaaa
     2401 aaccttttta ataaaatcaa atccattaat cctgatatct ttaccgtcgt tggcggccat
     2461 catgcaacgg ttgtccctga ggatttcttt actccctata ttgaccttat agtaatggga
     2521 gagggagttt ttgtattcaa ggagatcatt tcaaggcttg tttctaaaag accatttgat
     2581 ggaattgccg gaatatctta tttgaaaggt aaagagcggg taatgaatcc acctgaaccc
     2641 ataacggacc ttgatatctt tccttttcca gccagagaat ttacggaaaa atacagaaaa
     2701 tactattaca gtgaatggat gagacctctt gcatcaatac gaacatcaaa aggatgcccg
     2761 aataggtgta atttctgtgc tctgtggaag ctcacaggtg gaaagtatct gaaaagaaag
     2821 ccggaaaaca ttgtcaggga gctatccggg atcaaggaaa aatatgtttt ttttgcagat
     2881 gatgaatccc tggttgatgc cggcaggatg aagatccttg ctgaattgat taaggaagcc
     2941 ggaattaaga aacgttattt tctttatggg agaagcgata cgatcgtaaa aaatccggac
     3001 cttattaaaa tatggaaaga catcggactt gaaagggttt ttgtggggct tgaatttttc
     3061 agggatgaag accttaaata tataaataag ggttcaactg tcgaaaataa taaagaggca
     3121 attaaaatat tgaattcaaa tggcatagag atatatgctt cgtttattgt gaggccggat
     3181 tttactgaag aagattttaa agaatttaaa gaatattgtg ggaaaatgga gttcactttt
     3241 ctaagctttt ctgttcttac tcccttacca gggacggatt tgtatgaaga agttaaagac
     3301 cgtctcatta ctcatgatta tgatctcttt gattttattc atactcagct tccaacaaaa
     3361 ctccctt
//
LOCUS       DATGBN010000103         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_46842, whole genome shotgun sequence.
ACCESSION   DATGBN010000103 DATGBN010000000
VERSION     DATGBN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46842"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VKL21_02265"
     CDS             <1..814
                     /locus_tag="VKL21_02265"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HMB44624.1"
                     /translation="VLRYIDALVEGGIEVNTQIVLCRGLNDGIELEKTINDLSLLYPG
                     VHSISVVPVGITKYREERGLYNLKPFDTESSLDTIKIVEKLQKQYKKEKGSNIVYLAD
                     EFYLMSGKKIPGFKHYEDFPQIENGIGLIASFKYDLDNYLAKLKPNGNYTKNISIATG
                     TAAYEFINECVDKIKGKYINVTIKVYKIENKFFGEKVTVAGLLTGQDIISALQEKKLG
                     EFLFIPSSMLKHGEEIFLDDISIEDIERKLKVKVCSVKPRAYDLIDNVRGIK"
     gene            872..>1546
                     /locus_tag="VKL21_02270"
     CDS             872..>1546
                     /locus_tag="VKL21_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HMB44625.1"
                     /translation="MSRPIVAIVGRPNVGKSTLFNYIAGKKISIVDDTPGVTRDRIYA
                     EAEWRNRKFSMIDTGGIEPFSEDEIMIQMRRQAEIAIDTADVIVFMVDIKTGVTANDK
                     EVSIILKKSRKPVIIAINKADRLGGMPDDAYEFYNLGFENLACISSINALGIGDLLDM
                     IYDNFPKGNSDDSDDDVIKVAIIGKPNVGKSSLVNKICGENRVIVSDIPGTTRDAIDT
                     YVKKGEN"
BASE COUNT          583 a          199 c          323 g          441 t
ORIGIN      
        1 agtgctaagg tatatagatg ctctggttga gggtgggatc gaagttaata cgcagatagt
       61 cctttgccgc gggttaaacg atggaattga gcttgaaaaa acgataaatg acctaagctt
      121 attatatcct ggagtacata gtatatctgt tgtgccagtt ggaataacta agtataggga
      181 agagagagga ttgtacaatt taaaaccatt tgatacagaa agctccttgg atactataaa
      241 aattgttgaa aagcttcaaa aacaatataa aaaagaaaaa ggatcaaata ttgtatactt
      301 agccgatgaa ttttatttaa tgtcgggcaa gaaaatacca ggattcaaac actatgaaga
      361 ttttccgcaa atagagaatg gtattggcct tatagcatca ttcaaatatg atttagataa
      421 ctatttggcg aaactaaaac ctaatgggaa ttacacgaag aatattagta tagcaacagg
      481 tactgcggca tatgaattta taaatgaatg tgtggacaaa ataaaaggta aatatatcaa
      541 tgtcacaatc aaagtatata aaatagaaaa taagtttttt ggtgaaaaag tcacagtagc
      601 agggcttcta acagggcagg atataatttc tgcactccaa gaaaagaaat taggggaatt
      661 cttatttata ccttctagta tgctaaaaca tggagaagag atttttttgg atgacatatc
      721 tatcgaggat atagaaagaa aacttaaggt aaaagtgtgt tctgtaaagc caagagcata
      781 tgatttgatt gacaatgtgc gtggtataaa ataatataat gaagtatttt gtaaaaagaa
      841 atcgtattat gtcgaggaag gggaacaaaa aatgtctagg cctatagttg caattgttgg
      901 tagaccaaat gtagggaaat caacattatt taattatata gcaggtaaaa aaatatctat
      961 tgtagatgat actccaggag tcacgcgcga tcgtatttat gcagaagcag aatggagaaa
     1021 tcgcaaattt tcaatgattg atacaggagg tatcgaacct ttttctgaag atgaaataat
     1081 gattcagatg agaagacaag ccgaaattgc cattgataca gcagatgtca ttgtttttat
     1141 ggttgatatc aagactggag ttacagcgaa cgacaaagaa gtatcgataa ttcttaaaaa
     1201 atcacgcaaa cctgttatta tcgctattaa caaagccgat agattaggtg gaatgcccga
     1261 cgacgcatac gaattctata atttagggtt tgagaatctt gcatgtatat cctcaataaa
     1321 tgcactcgga ataggtgatc ttcttgatat gatatatgat aattttccta agggaaattc
     1381 tgatgattct gacgacgatg taataaaagt agctatcatc ggtaagccaa atgtagggaa
     1441 gtcgtcactt gtaaataaaa tttgtggtga gaatagagta attgttagcg atatccctgg
     1501 aacaaccaga gatgcaattg atacctatgt aaaaaaaggt gaaaat
//
LOCUS       DATGBN010000104         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_473924, whole genome shotgun sequence.
ACCESSION   DATGBN010000104 DATGBN010000000
VERSION     DATGBN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473924"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..525)
                     /locus_tag="VKL21_02275"
     CDS             complement(223..525)
                     /locus_tag="VKL21_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMB44626.1"
                     /translation="MTEHPLKIYEKLDPELLQHVEKSRDFAFDDGALLRKYKLLIGMA
                     FDASYGAVNGVISLAKQAIEAGATKEEITEALRVAQHLSGVGCVYTAAQALKELFG"
     gene            complement(548..1006)
                     /locus_tag="VKL21_02280"
     CDS             complement(548..1006)
                     /locus_tag="VKL21_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HMB44627.1"
                     /translation="MNGTTIQVTIKGVYLIEKAPGPVPIVLLEDESKRMMPIYIGISE
                     AISINSALKNEIPPRPMTHDLFVSLLSRLGSTIDDILIDELNEGVYYARMSIAVDGKR
                     FELDARPSDCIAIALRCSAPIHIRESVLSEAVITKEELQNVIPLESYISG"
     gene            complement(1055..1744)
                     /locus_tag="VKL21_02285"
     CDS             complement(1055..1744)
                     /locus_tag="VKL21_02285"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44628.1"
                     /translation="MITKLLSTGLPTLDTELDGGLLPGSMVYLTADPMTMGEIFLYQF
                     IQQRPSYYVSTERKPEHIINNIKQFGFEFSGIKFIDIHEKYYEKVGKLLDRGELRDYK
                     VLDSFIKQLELIDAKEINLIVDTITFFQHLEVKRSKFKELIDIMYETIKRTQGLGFLY
                     GIKSDNRSLIENEVINICDAVFDISLIRKADKTTTELTIPKARDRPIHGNILKFKIEG
                     GIIMDTSREIA"
     gene            complement(1759..2466)
                     /locus_tag="VKL21_02290"
     CDS             complement(1759..2466)
                     /locus_tag="VKL21_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44629.1"
                     /translation="MERVHTGVTGLDEMIGGGYPKGRTILILGTTGSAKTILGVSFIH
                     KACQEGRKSLIIATEELPEDIIAQAGSVGMSFEKFKTDGILAIEKIYEERTIHAKELL
                     AYGMDGSDKLQSNIIGLLDRISEDIDSVLIDNIGVFTLNMSPNEFRAQIDSLVVGLNK
                     RNITAVLIMDMSADSRTENIASYSVYGVLRTSIKDNPFIGSRERLLEVLKIRNTKISL
                     DPMRFEITSKGIELMNK"
     gene            complement(2521..>2707)
                     /locus_tag="VKL21_02295"
     CDS             complement(2521..>2707)
                     /locus_tag="VKL21_02295"
                     /inference="COORDINATES: protein motif:HMM:NF037269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HMB44630.1"
                     /translation="YVLINNKCPWGENGRVNPALCMMTKAIFARIGVHIYKDINLNLK
                     KTIAGKDGCCLIELILR"
BASE COUNT          734 a          576 c          486 g          911 t
ORIGIN      
        1 ttgtatgaac gtgaggaatc tcccggcttt agccgtggga gaggttcaat ttgtggaagt
       61 gaattcaact ttctacatca ctccatccat agaaatggtc aggtcatgga ggccaagagt
      121 ccctgacgat tttatgtttt caatttcgct tttgcagtga attaaaggcg aagattgtcc
      181 gataggaaat attcaagtgt ggaagacgtt atagggggat tatcaaccaa acaactcctt
      241 tagcgcctgc gctgccgtat acacgcagcc aaccccgctt agatgttgtg caactcttaa
      301 ggcttcggtt atctcttctt tcgtggcgcc cgcttctatc gcctgtttag ccaacgatat
      361 cacgccattg actgctccgt aggatgcatc gaatgccatg cctataagta acttatattt
      421 tcgcagcaga gcgccgtcgt caaaggcaaa gtcacgggat ttttcaacgt gttgaagaag
      481 ttcagggtct aatttttcgt agattttcaa gggatgttct gtcattttat tctctccaat
      541 attgatatta cccggaaatg taactttcaa gaggtatcac attttgaagc tcttcttttg
      601 ttattaccgc ttctgaaagc acactttccc ttatatgtat cggggcgctg catcgaagag
      661 ctatcgctat acagtcgctt ggtcttgcat caagttcaaa acgctttccg tccacagcaa
      721 tagacatcct tgcataatag accccttcat ttaactcatc tatcaggata tcatctatgg
      781 ttgaacccag gcgcgaaagt aatgagacaa aaaggtcatg tgtcatcggg cggggcggta
      841 tctcattttt aagagcggaa tttatagata tagcctcaga tattccgata tatatcggca
      901 tcatgcgttt tgattcatct tcaagcaata cgatcggtac cgggcctggc gctttctcga
      961 tcagatatac cccttttatg gttacctgta tagtagtccc attcataaag ttaatattaa
     1021 taagcgtggt attaagtttt cgcatcaaaa atattcatgc tatctctctt gaggtatcca
     1081 ttatgatccc gccctcaatt ttgaacttca ggatattgcc atgaatgggg cggtctcttg
     1141 cttttggtat tgtcaattcg gtagttgttt tatctgcttt tcttatgagt gaaatatcaa
     1201 agaccgcatc gcatatattt attacttcat tttcaataag agaacgatta tcacttttta
     1261 tcccatagag gaatccaagc ccttgcgttc tttttatagt ttcatacatt atatctatta
     1321 gttctttgaa tttgctcctt tttacttcaa ggtgttgaaa aaacgttata gtatccacaa
     1381 taaggtttat ttccttagca tcgatcaact caagctgttt tataaaagaa tccaggactt
     1441 tataatctct gagttctccc cggtccagaa gttttcctac tttttcataa tatttttcat
     1501 gtatgtcaat gaatttaata ccagaaaatt caaatccaaa ctgtttaata ttatttatta
     1561 tatgttcagg tttgcgttcc gtactaacat aatagctggg tctttgctgt ataaactggt
     1621 ataaaaaaat ctcccccatt gtcatggggt ccgccgtaag atataccatg gaacctggaa
     1681 gcagccctcc gtcaagttct gtatcaagag ttggaaggcc tgtggataat agtttggtta
     1741 tcatgaattc aatccttttt atttattcat aagttctata ccttttgagg taatttcaaa
     1801 tctcataggg tcaagagaga ttttggtatt acggatcttc aggacttcca aaagccgttc
     1861 tcttgatcct ataaagggat tatccttaat agatgtcctt agcactccat aaacagaata
     1921 tgaagcaatg ttttcggtac ggctgtctgc agacatatcc attatcaaca cagcagtgat
     1981 atttcttttg ttcaatccca ctactaaaga gtcgatctgt gccctgaact catttggtga
     2041 catattcagg gtaaaaaccc ctatattgtc tatcaaaacg ctatcaatat cctctgatat
     2101 cctgtccaat aagccaataa tattggattg cagtttatcg ctgccatcca ttccatatgc
     2161 caataattcc ttagcatgta ttgttcgctc ttcatagatc ttttcaatag ccaggatacc
     2221 atctgttttg aatttctcaa atgacatccc aaccgatcct gcctgtgcaa taatgtcttc
     2281 cggcagttct tcagtggcaa ttatcagtga ttttcgtcct tcctgacatg ccttatgaat
     2341 aaaacttacc cctaatatgg ttttagcaga ccctgttgtc ccgagtatca agatagtgcg
     2401 gcctttagga tatcctcctc ctatcatttc atcaagccct gtaacaccgg tatgtactcg
     2461 ttccataatc atgccaataa tatgtttaat cgttaataaa tatatgccat aaattgcgga
     2521 tcatctcagg attaattcaa tcaggcagca gccatccttt ccagctatgg ttttctttag
     2581 atttaaattt atatctttat agatatgcac accaattcgc gcaaaaatgg cttttgtcat
     2641 catgcaaagg gcaggattta cccttccatt ttcaccccat gggcatttgt tattgatcag
     2701 gacataa
//
LOCUS       DATGBN010000105         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_551481, whole genome shotgun sequence.
ACCESSION   DATGBN010000105 DATGBN010000000
VERSION     DATGBN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551481"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..445)
                     /locus_tag="VKL21_02300"
     CDS             complement(290..445)
                     /locus_tag="VKL21_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44631.1"
                     /translation="MENKEFNIELKNPRKPGRETKSSTAICPECGKPMCPKCNRHNVT
                     QLSGIAG"
     gene            complement(465..614)
                     /locus_tag="VKL21_02305"
     CDS             complement(465..614)
                     /locus_tag="VKL21_02305"
                     /inference="COORDINATES: protein motif:HMM:NF036315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5371 family protein"
                     /protein_id="HMB44632.1"
                     /translation="MKYVHVQSVLPQEDVIALKEKSGESSVKEAIVKAVYHYLKCNLV
                     NKDEE"
     gene            909..2012
                     /locus_tag="VKL21_02310"
     CDS             909..2012
                     /locus_tag="VKL21_02310"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMB44633.1"
                     /translation="MYEKIFEITDEIGPEKIIHLYEPRTKMKAIVVVDNVAAGPAIGG
                     VRMAPDVTLNEVRRLARAMTYKNIMAGLPHGGGKSGIIADPKIVNKEQVIRTFARGIK
                     NLLEYIPGPDMGTDESSMAYVYDETARAVGLPRELGGIPLDEMGATGYGVAECADVAK
                     DYINLELNGARVTIEGFGNVGKPAARYLSEKGAILVGASDSKGTIYNSKGINVEELIR
                     IKESMGSVINYKDGEVLKITDLLMINTDILIPAARPDVINDANSDVVDAKLIIEGANI
                     PITESAERMLHDRGILVVPDFVANAGGVITASVEYHGGTETVALDRIKTTIRRNTKEL
                     LDKVYSDKTYPRDTSVAIAKQRVMRAMKYREWM"
     gene            2003..2512
                     /locus_tag="VKL21_02315"
     CDS             2003..2512
                     /locus_tag="VKL21_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMB44634.1"
                     /translation="MDVMLSSIFEPESVAVIGASNNETKWGGRILKNLLSGFQGKIYP
                     VNPKETIIQGLDSYPSVLDIPGTVELGVIVVPSEHVHSVVEECGKKGVKGLVVISAGF
                     SEAGNEGAELELVSIARKYGMNMIGPNTLGIVNERVKLNASIIGKLPRPGSISFITQS
                     GSLGLALAE"
     gene            2543..>3323
                     /locus_tag="VKL21_02320"
     CDS             2543..>3323
                     /locus_tag="VKL21_02320"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44635.1"
                     /translation="MISTGNKAQTDDVDLIEYLSSDPSTGVIAMYIEGIKRGRKFLDA
                     ARNISKPIVAIKTGRSKRGAKAVFSHTGSIAGSDEVYSAAFKQAGIIRVDTIDELFDA
                     ALAFSCQPLPRGNNVAILSNGGGASIVAADECEKQGINITDLTEETREKIKKVIPEFA
                     SVSNPIDTAGTVSFKIYNDSIKALHDDPNVDAIIVIYVHTMISNAMPPAEAVVEMKQK
                     CDREKGKKPIIACWMGGLGTEEGVDLLKSGCIPNYPVPERAV"
BASE COUNT         1017 a          653 c          789 g          864 t
ORIGIN      
        1 tcgagagcta ctttgtccct gaaggcaagg gattggatat tgaacgtgaa ataaggaatg
       61 tttcggtcat ggtatctgcg gacaggacct gccgggcgct tatcaaggaa ttgtatattg
      121 atgatgagcc tttgacactc tcccacagct aaagccggag gattcttgtt tcatcgacta
      181 aacttatcat gattgagaat ttaaaaaaca tgacagaggt tataatctcg gcaagcgtta
      241 attcgggcat gccctgcagc tatgaatgaa aaatcttaat attcccgatt tatccggcta
      301 ttccggataa ttgggtgaca ttatgccggt tacatttagg acacattggt ttcccgcatt
      361 cagggcaaat tgcagtcgat gatttagttt ctctaccagg ctttcttgga ttttttaatt
      421 cgatattaaa ttctttgttt tccataattt ccttaaatca atttttactc ttcatcttta
      481 ttaaccaaat tacactttaa ataatggtag actgccttaa cgattgcctc tttgactgaa
      541 gactcgccgg acttttcttt caaagcgatt acatcttcct ggggaagtac tgattgaaca
      601 tgtacatatt tcataaaatc atatcctgag ataaaaataa ttttatctat taatattatc
      661 gaagtacaat tattgttttc aatattaaat gtatcgctat aaataaataa ttataattat
      721 ttacattgtg agcatgtatt aaaatctata aagtgtggct ggatgtttgt ttctgtaaaa
      781 tgtcctaaaa aatgcaggaa aatatatcga aatgatgatt caacctcttt tttctgggcg
      841 cataaccaca atcttaattt gcattccaga caagtaaata tatagactgg gagaggaaaa
      901 ccatggaaat gtatgaaaaa atattcgaaa taacagatga aataggccct gaaaagataa
      961 ttcatttata cgaaccgcgg accaaaatga aagccatcgt tgtagtggat aacgttgcgg
     1021 caggtccggc tatcggcggg gtgagaatgg ctcccgacgt aacgctgaat gaggtaagaa
     1081 ggcttgcccg tgcaatgacc tataaaaata tcatggcagg tcttccccat ggcggcggaa
     1141 aatccgggat catcgccgac ccgaagatcg tgaacaagga gcaggtgata aggacatttg
     1201 cccgcgggat aaaaaatctc ctggaataca ttccgggacc ggacatgggt acggatgaat
     1261 caagcatggc ctatgtttat gatgaaaccg cccgcgctgt cggccttccg agagaacttg
     1321 gaggcatccc gctggatgag atgggcgcaa ccggatatgg ggtagcagaa tgcgccgatg
     1381 tggcgaaaga ttacatcaat cttgaactta atggggcccg cgttaccata gagggatttg
     1441 gcaacgttgg gaaacccgca gcgcgttatc tttccgagaa aggcgcaata ctcgtaggcg
     1501 caagcgactc aaaaggcacg atatataatt ccaaaggcat taatgtcgaa gagttgatac
     1561 gcattaagga atccatgggt tcagtaatta attacaaaga tggagaagtt ttgaaaataa
     1621 ccgatctttt gatgataaat acggatatcc ttattcctgc ggccagaccc gatgtcataa
     1681 atgatgcaaa ttcggatgta gttgatgcaa aattgataat agaaggcgca aatattccta
     1741 taaccgaaag tgcagagagg atgctccacg acaggggtat cctggtcgtt cctgattttg
     1801 tagctaatgc aggcggggtt ataaccgcat ctgtcgaata tcacggcgga acggagacag
     1861 ttgcactgga caggataaaa acaaccatca gaaggaacac caaagagctc cttgataaag
     1921 tctatagcga caaaacatac ccccgtgata cttctgtcgc gatcgcaaaa caacgtgtca
     1981 tgcgcgcgat gaaatacagg gaatggatgt aatgctctct tccatatttg aaccggagtc
     2041 agtggcggta ataggagcgt caaataatga gacaaaatgg ggaggccgga tactcaaaaa
     2101 cctgttaagc ggtttccagg gaaagatcta ccctgtcaat ccaaaggaaa ctatcatcca
     2161 gggacttgat tcatatccat ccgttcttga tattccagga acagtggagc ttggggttat
     2221 agttgtccca tccgaacatg tccattccgt cgtggaagaa tgcggtaaaa aaggcgtgaa
     2281 ggggcttgtt gtgataagcg ctggctttag cgaggctggt aatgaaggcg ctgaacttga
     2341 actggtctct atcgcaagaa aatacggcat gaatatgata ggacccaata cgctgggtat
     2401 cgtcaatgag cgtgtaaagc taaatgcaag tattatcgga aaactcccgc gcccagggtc
     2461 gatatctttt atcacccaga gcggatcgct tggtcttgcg cttgctgagt gaactatcgc
     2521 aactgaactt ggtctatgca aagtgataag tactggcaac aaagcccaga cagatgacgt
     2581 tgaccttatc gaatacttaa gcagtgatcc gtcaactggt gtaatagcca tgtatattga
     2641 agggatcaag cgaggaagga agttccttga cgctgccagg aatatcagca aaccgatagt
     2701 agccatcaag accggaaggt ctaaacgcgg agcaaaggcg gttttttcac acacaggctc
     2761 cattgcaggc tcagatgagg tttattcagc ggcttttaag caggcgggaa taattcgggt
     2821 ggatactatc gatgagcttt ttgatgccgc tcttgctttt tcatgccagc ctcttccccg
     2881 ggggaataat gttgcgatcc tttcaaatgg cggcggagca tcaatagtag cagccgatga
     2941 atgtgagaag caggggataa atattacaga cctcacagag gaaaccaggg aaaaaataaa
     3001 aaaagtcatc ccggaatttg catcggtttc aaatccgata gatacggcag gaaccgtttc
     3061 atttaagatt tataatgatt ccattaaggc gcttcatgat gatccaaacg tggacgccat
     3121 tattgttata tatgtccata caatgatctc aaacgcaatg cctccggctg aagcagtggt
     3181 ggaaatgaaa caaaaatgtg accgggaaaa aggtaaaaaa cccataatcg catgctggat
     3241 gggtggcctc gggacagaag aaggggtgga tctcttaaag agcggatgta tccccaatta
     3301 tcctgtccct gagagagctg taa
//
LOCUS       DATGBN010000106         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_358028, whole genome shotgun sequence.
ACCESSION   DATGBN010000106 DATGBN010000000
VERSION     DATGBN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358028"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1042)
                     /locus_tag="VKL21_02325"
     CDS             complement(245..1042)
                     /locus_tag="VKL21_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMB44636.1"
                     /translation="MVANSGLFKYAFGIALGILILVLLPGNGASAALIIVPHDYARIQ
                     WAIDNATSGDTIVVHSGTYYENVKVNKKLILKGIGMPVVDARGRGSTIKLSKNGIRLE
                     GFRATGAGSRIENAGIKVKSNNNILIGNNATKNGNGERFGTGIYLYNSSNNTLKNNDA
                     SDNNPGGKYRHGIILESSDNNTLVHNKISNNEYGIFLLYSSNNTLNLNNVSYNIFGIF
                     LSNSNNNTLIRNKLSKNQVDFSLYYSSNNTIKDCKKKRGILTCYPHL"
     gene            complement(1128..2291)
                     /locus_tag="VKL21_02330"
     CDS             complement(1128..2291)
                     /locus_tag="VKL21_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB44637.1"
                     /translation="MRIAIFYDWINIIGGGEKLVLTMARGLGADVITTDLNIDSIKKL
                     GFEDVRIISIGNTIKLPILKQISLSMRFALCDFSRDYDFFIFSNNFSHFAARKHKPNL
                     WYCFSPPRVFYDLYDLFARKEPFIRRQIFKMWILIHESISRKFVDHVEYFVANSENVR
                     NRIKKYYGKDSIVVYPVIDISRYGFKKYGEFWLSVNRLYPEKRIELQVNAFRHMPDEK
                     LVIVGDFTKGDYSERYVSKILKDLPDNVKLIGSVAEDELIELYATCKGHITTALDEDF
                     GMTPLEAMASGKPTVAVREGGYLETIKDGVTGLLVDVDETAIINGVKLISMDPGKYRE
                     DCENRAKDFDIPAFIKQMENIIYSIQNPGDIQNKYNRDIEAAGGSKKIADEQI"
     gene            complement(2288..3373)
                     /locus_tag="VKL21_02335"
     CDS             complement(2288..3373)
                     /locus_tag="VKL21_02335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB44638.1"
                     /translation="MRIAIFHDYIQAIGGAEKVVLTLARELGADVITTDFDIKAVKKL
                     GFEDVKIITLGSTIKIAPLKQISACLKFALCDFSSKYDFFIFSNNWAHFAARKHKPNL
                     LYCQSTPVRVFYDLYDIYLRQQPFYMSILFKIWVALHKKFYEHFMDHVCVIIANSLSV
                     QMKVKKYLHRSSKVIYPPVNIHNFRFEKYGDFWLSVNRLYPEKRVELQIEAFRQLPNE
                     HLWIVGGFADADNSSTYAKKLKESLPENVKLLGSVTEEELIRFYATCRAYIITSFEEP
                     FGMAPVEAMASGKAVVGMREGGCLETVAEGATGLLVKPDVDEIVNAIKIISAEPSKFK
                     DKCIEQARKFDKYIFLSRIRTEIEELI"
     gene            complement(3370..4500)
                     /locus_tag="VKL21_02340"
     CDS             complement(3370..4500)
                     /locus_tag="VKL21_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB44639.1"
                     /translation="MKIAFVYDAIYPWVKGGAEKRIYELGKRLAANGNEVHIFGIKWW
                     DGANIIKNEGMVLHGVCGPMELYVNGRRSFYEAIYFSIRLLPHLIKESFDVIDVSVFP
                     YFSCFIVKIVSIYKRIPMVTTWHEVWGDYWYEYLGKIGSVGKFIEQVVFRMSNHSIAV
                     SNHTKKSLESLGIDQNNIYIVTNGIDMKTINQIKPSIYTCDIIFIGRIIKEKNVDILI
                     ESVHNIKKYLPDIQCHIIGGGPEEKKLKNLICDLGLQDNIKFFGFLDYIDVIAMLKSS
                     KVLVLPSTREGFGIVIIEAFACGVPVITVRSPRNAANELVNKDAGFVVDLNAREIGDS
                     IYKVITDGRLHKKMSFSAISLTQEYDWDKKTIQLFNIYNGLK"
     gene            complement(4497..>4987)
                     /locus_tag="VKL21_02345"
     CDS             complement(4497..>4987)
                     /locus_tag="VKL21_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB44640.1"
                     /translation="RHLRFMMLYNPTPFFSIPGFLILVFGIFMTLTLAIRGNVQTTSL
                     HSFILGLMMLIIGAQMIATGGYMKVYGIIHNRIDKTGLTSKFLDYHSLEFGLIFGFIL
                     FFAGLVLGTGVVLKWINSGYGSLSEVGTAVTSVTMAALGIQVIFSTLIISIFLLEQKD
                     IQ"
BASE COUNT         1468 a         1063 c          833 g         1623 t
ORIGIN      
        1 atgtctggaa ggttaatgta gagttcaaag agaagactgg tgctatagaa atgcctacaa
       61 cagcgttatt tattttggat gcaatgacag gggaagtgaa ggaatttaag aagggttatg
      121 catcgacatt ttgatgaaat aacattcaaa atttattact atgtttgatg ctgatgctct
      181 cacgtagcct atttttgatt gcccctattt tgaaaaatgg agaaacgggt aatacttcgg
      241 actattacag atgtggataa caagtgagta ttcccctttt tttcttgcag tccttgatgg
      301 tgttgttgct ggaatagtac aaagaaaagt ctacctggtt ctttgaaagt ttgttcctga
      361 tgagcgtgtt attattggaa tttgacagaa agatcccaaa tatgttgtat gatacattgt
      421 tcaagttcag tgtgttgttg ctggaatata acagaaagat gccatattcg ttgttggata
      481 ttttgttgtg taccagtgta ttgttatccg atgattccag aatgatgccg tgccgatact
      541 ttccgccggg gttattgtcg gatgcatcgt tgtttttcaa cgtgttgttg ctggaattat
      601 acaggtaaat gcctgttccg aatctctccc cattcccgtt tttcgtggca ttattaccta
      661 tgagtatatt gttgttggat ttgaccttga ttccggcatt ttcgatccgg gagcctgctc
      721 ctgttgccct gaatccttca agtctaattc cattcttaga aagcttgatc gtgcttcccc
      781 ttcccctggc atccacaaca ggcattccaa tacctttaag gatcagtttc ttgtttacct
      841 tcacgttctc gtaataagtg ccgctatgca ctacgatagt atccccggag gtcgcattat
      901 caatagccca ttgtatcctg gcatagtcat ggggaactat aattaatgca gcactcgcac
      961 cattcccggg taataatacc aatattaata ttccaagagc tattccaaat gcatacttga
     1021 ataaacccga attcgccacc ataattccat atccactttc gatgagtgca ccataccgtt
     1081 tttctaagca atcccactcg tattatatca tattggattt gaagggctta aatttgttca
     1141 tctgcgattt tcttgcttcc tccagcagct tcaatgtccc tgttgtattt gttttgaatg
     1201 tcgcctgggt tttgaattga atatattata ttctccattt gcttaataaa tgctggtata
     1261 tcaaaatcct tggccctatt ttcacaatct tccctatatt ttcccggatc catagaaatt
     1321 aatttcactc cattgataat agcagtttca tcaacatcca caagtaaccc ggttacacca
     1381 tccttgatcg tttccagata acccccttct ctgaccgcaa ctgtaggttt acccgaagcc
     1441 attgcctcga gaggggtcat tccaaagtct tcatccagcg ctgttgttat gtgtccttta
     1501 caggttgcat ataactcaat aagctcatct tccgcaacgc tgccgatcaa tttcacatta
     1561 tccggaagat ccttgagaat tttggataca tatctttctg aataatcacc tttagtaaaa
     1621 tccccaacaa ttaccaattt ttcatccggc atatgtctga aagcattgac ctgtaattct
     1681 atacgttttt cagggtataa acgattcaca gaaagccaga attctccgta tttcttaaac
     1741 ccgtatcttg aaatgtcaat aacaggatat accactattg agtccttccc ataatatttc
     1801 ttgatcctgt tccggacgtt ttcggagttc gcaacaaaat attcaacatg gtctacaaat
     1861 ttgcggctga tcgattcgtg tattaagatc cacatcttaa atatttgccg ccttatgaaa
     1921 ggttcttttc gtgcgaacag gtcatataga tcataaaaaa ccctgggagg tgaaaaacaa
     1981 taccagagat tgggtttgtg ttttctggcg gcaaaatgtg aaaaattatt tgaaaaaata
     2041 aaaaaatcat aatccctgga aaaatcgcat agagcaaacc gcattgataa agatatttgc
     2101 ttaaggatcg gcaatttgat tgtattcccg atactgatga ttctaacatc ttcaaatcct
     2161 aatttcttaa tcgaatcaat attcaggtca gtggttataa catcggcacc aagtccccgt
     2221 gccatggtta aaactagttt ctctcctcct ccgataatat ttatccagtc atagaatatt
     2281 gcgattctca tattagttct tctatttctg ttctaatcct gcttagaaag atatatttat
     2341 caaattttct agcctgttct atacatttat ccttgaattt tgatggttct gcggatatta
     2401 tttttatagc attaacgatc tcatccacat caggtttcac aagtaaccct gttgcaccct
     2461 cggcaacagt ctctaaacac cccccttccc tcatccctac gaccgccttt cctgaagcca
     2521 tggcttcgac aggcgccatg ccaaaaggtt cttcaaaaga tgtgataata tatgccctgc
     2581 aggtggcata aaaacggatc aattcttcct cggtcaccga tcccagcaat tttacatttt
     2641 ccggaaggga ttctttcaat ttttttgcat aagtagaaga attatcagcg tctgcaaaac
     2701 cacccactat ccacagatgt tcatttggaa gttgcctgaa tgcttcgatt tgcaattcga
     2761 cgcgtttttc gggatataac ctgtttacag ataaccagaa atccccgtat ttttcaaatc
     2821 taaaattatg tatattgaca ggcgggtaaa tgactttcga agatctatgc aaatattttt
     2881 ttaccttcat ttgcacactt aaggaattag ctattataac acaaacgtga tccataaaat
     2941 gttcataaaa ctttttgtgc aacgctaccc agattttaaa caatatggac atataaaatg
     3001 gttgctggcg taagtaaata tcatacagat cataaaaaac tctcactggc gtcgactggc
     3061 agtataacag gttaggctta tgcttccttg ccgcaaaatg tgcccagtta ttcgaaaata
     3121 tgaaaaagtc atattttgat gaaaaatcgc aaagagcaaa cttcaaacat gctgaaatct
     3181 gtttaagagg cgcgatttta attgtactgc caagggttat gattttcaca tcctcaaagc
     3241 ccagtttttt taccgccttt atatcaaagt ctgttgttat aacatctgct cccagctcac
     3301 gggcaagtgt caatacgacc ttttctgctc cccctatagc ctggatataa tcatgaaata
     3361 ttgcaattct cattttaatc cattatatat gttaaaaagc tgtatggttt ttttatccca
     3421 gtcatattcc tgagtcaggc taatcgcaga aaatgacatt tttttatgga gtctgccatc
     3481 tgtaattact ttatatatag aatccccaat ttctctcgca ttgagatcta cgacaaaacc
     3541 tgcatctttg ttaaccaatt cattagctgc atttcgggga cttcttacag tgattacagg
     3601 cacaccacac gcaaaagcct caataataac aatcccaaaa ccttcacgcg tggaaggaag
     3661 aactaacact tttgatgatt ttaacatcgc gattacatca atataatcca gaaaaccgaa
     3721 aaattttata ttatcttgaa gccctaaatc gcagatcagg ttcttaagtt ttttttcttc
     3781 aggtccaccg ccaataatat gacattgtat atctggcaga tattttttaa tattatgaac
     3841 cgattcaatt aatatatcca catttttctc cttaataatc ctgccaataa atattatatc
     3901 acatgtataa atagatggtt ttatttgatt gatcgttttc atatcaatgc catttgtcac
     3961 tatgtagata ttgttctggt cgatacctaa tgattccaga ctttttttag tatgattaga
     4021 tactgcaatc gaatggtttg acattctaaa aaccacttgt tcaatgaatt tcccaaccga
     4081 accgattttt cccagatatt cataccaata atctccccat acctcatgcc atgttgtgac
     4141 cattgggatt cttttatata tcgatacgat cttcactata aaacaagaga aatagggaaa
     4201 tacgctgaca tcgataacat cgaaactctc tttgatcagg tgcggtaaga gtcggattga
     4261 aaaatatata gcctcataga acgatcgtct gccattaaca tatagttcca ttggaccaca
     4321 tactccatgc aacaccattc cctcattctt tattatattg gcgccatccc accactttat
     4381 tccaaaaata tgcacttcat tcccattagc cgccaatctt ttacccaatt cataaatcct
     4441 cttttcagcg ccgcctttga cccatggata aatagcatca tatacgaatg caattttcat
     4501 tgaatatcct tttgttctaa taaaaatata cttataatga gcgttgaaaa tattacctgg
     4561 ataccaagcg ctgccatcgt cactgaggtc actgcagtcc caacttctga aagcgaaccg
     4621 tatcctgagt ttatccattt caagacaaca cctgttccca ggacaagacc tgcaaagaat
     4681 aatataaatc caaagatcaa tccgaattcc agggaatggt aatctaagaa ttttgacgtc
     4741 aatcctgttt tatcgatcct gttgtggatt atgccgtata ctttcatata gccgccagtg
     4801 gctatcatct gggcgcctat aatcagcatc atcaaaccaa gaataaaaga atggaggctt
     4861 gtggtttgaa cgttcccgcg aattgccagt gtaagcgtca taaatatgcc aaagaccagg
     4921 attaaaaaac ccggtattga aaaaaaaggt gttggattgt aaagcatcat gaaccttaaa
     4981 tgccgcc
//
LOCUS       DATGBN010000107         5575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_474335, whole genome shotgun sequence.
ACCESSION   DATGBN010000107 DATGBN010000000
VERSION     DATGBN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474335"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VKL21_02350"
     CDS             complement(<1..279)
                     /locus_tag="VKL21_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HMB44641.1"
                     /translation="MKEIIFVGRSNVGKSTLIRALTRKKVPVGKLPGVTRKPLHIPYQ
                     NIQITDMPGFGYMSKVTRKGQEAIKDNIVHYIEDNSSNILLAVMVVNTT"
     gene            369..1196
                     /gene="proC"
                     /locus_tag="VKL21_02355"
     CDS             369..1196
                     /gene="proC"
                     /locus_tag="VKL21_02355"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HMB44642.1"
                     /translation="MTTTGMANKKIGFIGTGKMGEALIKGILHAGLVPPGNIYASDMD
                     VAKLQMLEKELKINICKDNCDAVANSDVLIIAVKPQIVPAVLNELKSSIKNQLIISIA
                     AGVTIETFEKNLLPGTKVLRVMPNIAATVKEAASAISPGSAVSKEDTAMAVKIFNAIG
                     RTVILQENLMDAVTGLSGSGPAYIFMIIEALADGGVHEGLDRNTAKLLAAQTVLGAAK
                     MVLEGPGHTGELRDMVTSPGGTTIRGLRVMEEQGIRIGMMNAVIASCERSKELGKKT"
     gene            1206..2009
                     /gene="mtxX"
                     /locus_tag="VKL21_02360"
     CDS             1206..2009
                     /gene="mtxX"
                     /locus_tag="VKL21_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HMB44643.1"
                     /translation="MSLLFDIEIKARKNHACIGLGIGKVSEKLIRGAESASEYAHVVL
                     IGDEMEIKAIGTDLEVIHSNEPSKKLIELLLNGKIDGAVRGTLSATKTLSQLKKSLNV
                     TKLYRVALLETADGRPFFLAPVGIDEGNSVDDKVELIKRASRHFERLGVKMRVGILSG
                     GRFEDIGRDKTVDRTLEDAQLVTGKVRELGIEVKNYSILIEDAIEEANFIMAPDGISG
                     NLIFRTLVFLGGGYGHGAPVLMEKVFVDTSRVGGHYTRAIILASALKHT"
     gene            2012..2815
                     /locus_tag="VKL21_02365"
     CDS             2012..2815
                     /locus_tag="VKL21_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HMB44644.1"
                     /translation="MSIRSLLRDKLPPEKLALLPKGFEVIGDIAIINIPLPLYEERYL
                     IAEALLIQRKDIKTVLRKLNKIEGSARVADFERLLGSRTTTLHRENGCIFQLDVTKTF
                     FSGKMYYERGRIAQKVEDGEDVLVLFSGVGPFLIPIKKKRNVSITGLDNNREACTFLR
                     KNLGLNHIEANIILGDARQINNLFKTRFDRIVMPAPYGQDFFLDMAQFILRPGGCVHF
                     YTFKKDFEIPHFRRLLEEKGWQIDFTRDCGDVAPRVKRYVFDLQLGNTE"
     gene            2889..2960
                     /locus_tag="VKL21_02370"
     tRNA            2889..2960
                     /locus_tag="VKL21_02370"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2922..2924,aa:Gly,seq:gcc)
     gene            3099..3593
                     /locus_tag="VKL21_02375"
     CDS             3099..3593
                     /locus_tag="VKL21_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMB44645.1"
                     /translation="MATISHNIKRARTHIYPGAHTLNAYITIKGCNEAIEFYKKAFGA
                     KERGRIVMPNGFIAHAEVEIEGSLLMMWDENVEWGSVSPTTLGGNPVTLGLYVQDVDV
                     VFKQAVDAGATLVQPVTDTFYGDRSGVVMDPFGYKWLIATHKEDVSFEEMKKRSNAIL
                     GEKK"
     gene            3675..4490
                     /locus_tag="VKL21_02380"
     CDS             3675..4490
                     /locus_tag="VKL21_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMB44646.1"
                     /translation="MDEQQLKVMLKETFDTVSGGYDGKALRFFQASAEHMAFLLGLQG
                     NENVLDVACGTGHAALAIAKFLPTGWVTAVDFSSGMLDQARRKAASLDVHNVEFIEQD
                     MQNLNSSDGLFDVAVCSFGIFFVEDMESQLLRIASMVKPGGRIMISNFQENYFHPLKD
                     MFYERLEAYGVQMPPHTWKRIASEMGCLQLFEYAGLTNITVTRKNVGYHLDSAEEWWD
                     VVWNAGFRRMVSQLSTKDQEQFKSEHLDEVETLRTRDGIWLDVGVLFTCGKKN"
     gene            4804..5163
                     /locus_tag="VKL21_02385"
     CDS             4804..5163
                     /locus_tag="VKL21_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44647.1"
                     /translation="MQEEQEEIKEIKRTLSDLTVKFEDKFDELSKSVEEQQENAEEKI
                     NENTTQEGRIASLESTRASGHATTQAGLLLRRMDSLEKHAGATTSPDPGVAALEPQLE
                     GLETRIATLEHKKQSDK"
BASE COUNT         1689 a         1112 c         1367 g         1407 t
ORIGIN      
        1 tgtagtattt acgaccatca ctgccagcag gatatttgaa gaattatctt ctatataatg
       61 aacaatatta tcctttattg cttcctggcc ttttcttgtt actttgctca tgtacccgaa
      121 tcccggcata tctgtaattt ggatattctg ataaggtata tgaaggggct tcctcgttac
      181 accgggaagc tttcctacag ggactttctt tcgagtaagt gctcttataa gagtggattt
      241 gcccacattt gatcgtccca caaaaattat ctctttcatc aaccaactgc ataatctagg
      301 tcatataaaa acttcgccat gaatgataat ccttttatta cttcatgtct ttaatgtgtt
      361 agatctcaat gacaacaaca ggcatggcaa ataaaaaaat cggatttatc ggcacaggaa
      421 aaatgggaga agcccttatt aaaggcatac ttcatgccgg gcttgttccc ccagggaaca
      481 tttatgccag tgacatggat gttgcgaaat tgcagatgct tgaaaaggag cttaaaatca
      541 atatctgcaa agataattgc gatgcagttg caaattcgga tgtacttatt atcgcggtaa
      601 aaccgcagat cgttccggca gttctaaatg aactcaaaag ttcaataaag aaccagttaa
      661 taatatcaat agcagccgga gttaccatag aaacatttga gaaaaacctg cttccaggga
      721 caaaagtttt aagagtgatg cccaatatcg ctgcaacagt gaaagaagca gcatcagcaa
      781 taagtccggg aagcgcggta tcaaaagaag acactgcaat ggcggtcaag atattcaatg
      841 ctattggcag gacagtaatt ctccaggaaa accttatgga tgccgtgacg ggattatcgg
      901 gaagcggccc ggcgtatatt ttcatgataa tcgaagccct ggcagacgga ggtgtgcatg
      961 aagggctgga caggaacact gcaaaactgc ttgcggcgca gaccgttctt ggcgctgcca
     1021 aaatggtgct tgaaggcccg ggtcacacgg gagaattaag ggatatggtg acatccccgg
     1081 ggggcacaac catccgggga cttcgggtca tggaagaaca gggtatcagg atcggcatga
     1141 tgaatgctgt gatcgcgtcc tgtgagcgtt caaaagagct gggcaaaaaa acttgaaact
     1201 caaaaatgtc actacttttc gatattgaga taaaagcaag aaagaatcat gcctgtattg
     1261 gcctcgggat aggtaaagtc tctgaaaaac tgataagggg cgcggaaagt gccagtgaat
     1321 acgcccatgt tgtgctcatt ggggatgaaa tggagataaa agccatagga acagaccttg
     1381 aggtaataca ttcaaatgag ccctcaaaga aattgatcga attgctgcta aacggaaaga
     1441 tagacggggc cgtcagggga acgctcagcg ccacaaagac gctttcccag cttaagaaat
     1501 cgcttaatgt tacaaagctg tacagggtgg cattattgga aacggcagac ggaagaccat
     1561 ttttccttgc tcctgtgggt attgacgaag ggaattccgt tgatgataag gtggagttga
     1621 taaagcgcgc ctccaggcat ttcgagcgcc ttggtgtgaa aatgagggta gggatcctct
     1681 caggcggacg ttttgaggat ataggaaggg acaagaccgt ggacaggaca cttgaagatg
     1741 cgcaacttgt gaccggaaaa gtacgtgaac tggggatcga agtaaagaat tattccatcc
     1801 tgatagaaga tgcgatcgag gaggcaaatt tcattatggc gccagatggg atttcaggga
     1861 accttatatt caggacgctt gtgttcctgg ggggaggata cggtcacggc gctcctgtct
     1921 taatggagaa ggtcttcgta gatacttcgc gtgtgggagg gcattatacg agggcgataa
     1981 tacttgcaag tgcgctaaag catacctgaa tatgtccatt cgttccctcc tccgggataa
     2041 gctccccccc gaaaaactcg ccctcctccc taaaggattc gaagtaattg gagacatcgc
     2101 gataataaat atcccactcc ctctttatga ggaaagatat cttattgcag aggcactgtt
     2161 aatccagagg aaagatatca agaccgtcct gcgaaaactc aataaaatag aaggctctgc
     2221 acgggttgcg gactttgaac ggctgctggg gagcaggaca accacgctgc acagggagaa
     2281 cggctgcatt tttcagttgg atgtaacaaa aacgtttttt tcaggaaaaa tgtattatga
     2341 aaggggaagg atagcgcaaa aagtagaaga tggcgaggat gtacttgttt tattctcagg
     2401 tgtcgggcct tttcttatcc caataaagaa aaaaaggaac gttagcatca caggacttga
     2461 taacaaccgg gaagcttgca cttttctccg gaaaaatctt ggactgaacc atattgaagc
     2521 gaatattatc cttggtgatg cccgacaaat taataacctt ttcaaaacaa ggtttgaccg
     2581 tatcgtgatg ccagcgcctt acggacagga cttcttcctg gatatggctc aatttatttt
     2641 aaggccaggg ggatgcgtcc acttttatac tttcaagaaa gatttcgaga tacctcactt
     2701 cagaaggctc cttgaagaaa aaggatggca gatcgacttt accagagatt gcggggatgt
     2761 ggcgccaaga gtgaagaggt atgtttttga cctgcagttg gggaatactg aatgaactgt
     2821 ggggtacgat tagaaaaact attaatatgg attgaaatat taagaggatg cccgcaaagc
     2881 ttcggatcgc gccggtagtg tagcggttat cactaggcgt tgccaacgct taaactcggg
     2941 ttcgagtccc gaccggcgca ttttttttta tgcatacaaa agatttctca caattaactg
     3001 aagctgaatt ggacaaatta catggcgtgg ggcctaattc gaggggcgac tcagttacgc
     3061 gaaagattat cttttgctaa tgctgttgaa gtagtactat ggcaacaatc tcacataata
     3121 taaagcgagc tcggacacac atataccctg gggcacatac actaaatgca tatattacga
     3181 taaaaggatg taacgaagct atcgaatttt acaaaaaagc cttcggtgcg aaagaacgag
     3241 gtagaatcgt tatgccaaat ggattcatag ctcacgcaga agtagagatt gagggatcgc
     3301 tacttatgat gtgggacgaa aatgttgaat ggggtagtgt aagccctact actcttggtg
     3361 gaaatcccgt cactcttgga ctgtatgtac aggatgttga cgttgttttc aagcaagctg
     3421 tggatgcggg agcaacatta gtgcaacccg ttactgacac attttatgga gatcgttctg
     3481 gagtggtaat ggatccattt ggatataaat ggcttatcgc cacacacaaa gaagatgtga
     3541 gtttcgagga aatgaaaaaa aggtcgaatg cgatcttggg agaaaagaaa taagtgattt
     3601 aaggaagtat tgagtttgta tggaccaaaa accatttaaa caaactctcg acttttattc
     3661 tcttcaggta taatatggac gaacagcaac tcaaagtaat gttaaaagag acattcgaca
     3721 ctgtatcagg cggatacgac ggcaaagcgc tccgcttctt tcaggcaagc gctgagcaca
     3781 tggctttcct tctcggattg caaggcaacg agaatgtgct cgatgttgcc tgcggaaccg
     3841 ggcatgcggc actggctata gccaaattcc tgccgaccgg atgggtaaca gccgtggatt
     3901 tctcatcagg catgcttgat caggcgcggc gtaaggcggc ttcactcgat gtccacaatg
     3961 tcgagttcat tgagcaagat atgcaaaatt tgaattcctc agacggtcta ttcgatgttg
     4021 cagtttgttc cttcggaatt ttttttgtcg aggacatgga aagccagctt ttgcgcatcg
     4081 cctctatggt caaacccggc ggcaggatca tgatctctaa tttccaggaa aactactttc
     4141 acccgttaaa ggacatgttc tatgaacgac ttgaggcata cggtgtgcag atgccgcctc
     4201 atacctggaa acgcattgct tctgagatgg gttgcctaca gctctttgag tatgccgggc
     4261 ttacgaatat caccgtcacc cggaaaaacg ttggatatca cctggacagc gccgaggagt
     4321 ggtgggatgt agtctggaac gcgggatttc gcaggatggt cagccagctt tccacgaaag
     4381 accaggaaca gttcaaaagc gagcatttgg acgaagttga aaccctcaga accagggacg
     4441 gtatatggct agacgtgggt gtgctgttca cctgtggcaa aaagaattga attaatccca
     4501 aaggacatga tgcattcaat gaggtgataa acgccattga agaacctctg cctgaaggac
     4561 agaggttgaa tttacccctt gaccccagca taattaagcc atttccccgc taatttcccc
     4621 gcttaatcaa atacagttag tttcttatgt tagttatact tatccattcg tttgaaagaa
     4681 aatgaacgtt cagatatttt taaatactac ataaacctat agtatattgt ttgccacaaa
     4741 tgaacaaaat gtgagggaat ttaccttgac aaactttttt cgttacattg tggaggttaa
     4801 tttatgcaag aagaacaaga agaaataaaa gagataaagc gtacactcag tgatctgacc
     4861 gtcaaatttg aagacaaatt tgatgaacta tctaaatcag tcgaagagca acaagaaaat
     4921 gctgaagaaa agataaatga aaacacaaca caggagggac gtatcgcctc gctggagagc
     4981 acgcgtgcca gtgggcatgc tacaactcaa gcgggtttgc tgttgcgacg tatggactcg
     5041 ctggagaagc atgctggagc gaccacctcg ccggacccag gcgtcgctgc tctggagccg
     5101 caactcgaag gtctggagac acgcatcgcc accctggagc acaaaaaaca gtcagataaa
     5161 taagaataat gttccacaca caataacttc cgtatcaggt tcattcgatt caggaagtat
     5221 cgcttcaggc aaaacataca gttataaatt taatcaagcc ggaacatttg aatataactg
     5281 tacaaaccat ccaagcatgc cgcacgggaa agtaggcgtg agaggttaaa tcatgatcgg
     5341 gacaataatt gtaattctga ttatactctg gctattggga ttttcgctcg gtttggttgg
     5401 tagctttatc catatattac tggtcatcgc tatcatcctg atattgctca gaatcatcca
     5461 gggacgaaaa cctttgtagc tagtgtagaa gaggaagcaa tggaaactga atagcaaatg
     5521 gggtgttttt gggaaacgca ccgaaaaggt aaaataagga attttcaagc gttag
//
LOCUS       DATGBN010000108         4760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_125232, whole genome shotgun sequence.
ACCESSION   DATGBN010000108 DATGBN010000000
VERSION     DATGBN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125232"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1661
                     /locus_tag="VKL21_02390"
     CDS             183..1661
                     /locus_tag="VKL21_02390"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HMB44648.1"
                     /translation="MTSEILSLAKYLASSRLEFSLSEMNQYLNKKYEEEELYAVLKPH
                     FFDLEIFCGAGYECTKLIKSPLPNDEVVIRELEALFKNPGLPLRIEKLIETYIEKSCG
                     KNWHKGETAALIRENIVKQKEEYWKGKGESRYPKIRIISYLLYHFPVYFCQYQYLLLG
                     LFKAGLLMNKMSIVDVGSGPGTITLGTLDFFRKLQDVYSRNKMNVKMNIRMGSIEYET
                     ENIECYKELTSNFLSRGISNIIIDEPVHSFIETAPVPKEADLIIFSNVLAELRAPPHE
                     RAGIVERIASSSKNPTLIIIEPADLDNSKALRIAQHALIDKGFNVYSPCSFIWGKVCS
                     GVNCWSFQEPGNIKASGFMEKIADTMESYRYLNTDMKFSYVILRKDGFVKHAYRAKGK
                     FMALSNLKKHIKKHINVAASVMSGNLGDEKTFVFKICDGSTSIPCYAVMPAYHLSENN
                     KVLIDAFYGDIVEIYGTLVRENKEQSSINLLVTRNTIVNFAG"
     gene            complement(1658..2062)
                     /locus_tag="VKL21_02395"
     CDS             complement(1658..2062)
                     /locus_tag="VKL21_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HMB44649.1"
                     /translation="MVEKDSDDKIQKITKLLEKGGTMLATHHECGAPMFRYQGKIVCP
                     VCDFQEEKQKNEPQETGKTEKPQIGHKKADQNRIVRAEPLSFDTSAINNMIINKVREL
                     AISLETEADLGRTRDKMECIEQGLKILKLLQD"
     gene            2131..2409
                     /locus_tag="VKL21_02400"
     CDS             2131..2409
                     /locus_tag="VKL21_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HMB44650.1"
                     /translation="MITVSENPSEVIKQCTEVLERIVGDDSVPRNIRRSADNIKNVLS
                     NEKQSLSKRAAMVISNLDDMGNDPNIPIHTRTLIWGLSSKLESIPVDR"
     gene            2947..4188
                     /locus_tag="VKL21_02405"
     CDS             2947..4188
                     /locus_tag="VKL21_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HMB44651.1"
                     /translation="METKTSSINGIFEDMLMRESIFENREVLRSTYTPDYLPHRDEQV
                     KGLATILVSALRGETPSNILIYGKTGTGKTAVAKHVGKELEKTSEMHSVPCVVVYINC
                     EVVDTQYRLLASIARHFEKDIPMTGWPTDQVYTELKSALDSDKRIAIIILDEVDKLVC
                     KGDDVLYNLTRINSDLKKAKVSLIGVTNDLKFTEFLDPRVKSSLGEEEIIFPPYDANQ
                     IRDILNQRAHMAFKPQVLEETVIPLCAAYAAQEHGDARRALDLLRVSGEIAERIKSNK
                     VLEAHVKQAQEKIEIDRIVEVVRTLPSQSKLVLLSVVLLGYNGESNLTTGEAYNIYKQ
                     MCRMIGIEILTQRRVTDLISELDMMGILNATVVSKGRYGRTKEISLSVPSESTKRVLF
                     EDYRLKPLEDFRPPNQTKLYA"
     gene            complement(4239..4760)
                     /locus_tag="VKL21_02410"
     CDS             complement(4239..4760)
                     /locus_tag="VKL21_02410"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMB44652.1"
                     /translation="MRDFLFVLTVVIIFASVSKIALGLYTPMVAVESGSMIPNIQIGD
                     IIFVKSADRTEIITNEAGKKLDYSSFNEYGDVILYKPLGRVEATPIIHRAMYYVDNGD
                     PMWSGGPPAPYAGYITKGDNSKTNAAYDQQGSISYMQPVKKEWVIGKAIFTRVPFLGC
                     ISLIPRGNLACFK"
BASE COUNT         1441 a          915 c          982 g         1422 t
ORIGIN      
        1 catagacatt gtaagtttga gagtttcttt atcatactca gacaaatcca atttaagctt
       61 tatcgttctg tccacattca tatattacca ctcataaacg taattagttt atggtatcgg
      121 gaggcggggt acgctcctcc ctgcattgaa atgcaggatc ttcgcacccc atggaggagc
      181 aaatgacatc agaaatcctc tccctcgcaa aataccttgc ctcatcccgc ttggagttct
      241 cgctatctga gatgaaccag tatcttaaca agaaatatga agaagaggag ctctacgctg
      301 ttctgaaacc gcattttttt gaccttgaaa tattttgcgg cgccggctat gaatgcacca
      361 aactcataaa gagccctctg cctaatgatg aagttgtaat cagggaactt gaggcgctgt
      421 tcaagaatcc gggattgcct cttcgcatcg aaaaattgat cgaaacttat atagaaaaat
      481 cctgcgggaa gaactggcac aaaggcgaaa cagcagcgct gatccgtgag aatatcgtta
      541 agcagaaaga ggaatactgg aaaggaaaag gagaatccag gtatccaaag atcaggataa
      601 tatcctacct gctctatcat tttccggtat atttctgcca gtaccagtac ctcctgcttg
      661 gattattcaa ggccggattg ctaatgaaca aaatgagcat cgttgatgtt ggttcgggtc
      721 ccgggacgat aacacttgga acacttgatt tctttaggaa attacaggat gtctattcca
      781 ggaataaaat gaatgttaag atgaatataa ggatggggtc gatcgaatat gagacggaaa
      841 atatcgaatg ttataaggag ctcacatcca atttcctatc gcgcggcatt tcaaatataa
      901 taattgatga acctgtccac tcatttattg aaaccgcgcc tgttccaaag gaggctgatc
      961 ttataatttt ctcgaatgtt ttggctgagt taagggcgcc accccatgaa cgcgctggta
     1021 tagtggaaag aatcgcttct tcaagtaaaa atccaacact cattattatt gagccggctg
     1081 accttgataa ctcaaaagcc ctcaggatcg cccagcatgc tctgatcgac aaaggattca
     1141 atgtctacag tccatgctca tttatctggg gaaaggtctg cagcggagtc aattgctgga
     1201 gtttccagga acctggaaac ataaaggcgt ctggtttcat ggaaaagatc gctgatacaa
     1261 tggagtctta cagatactta aacacggata tgaaattttc ctatgtaata ttaaggaagg
     1321 atgggtttgt taaacacgct tatagggcaa agggaaaatt tatggctctt tcaaacctga
     1381 aaaagcatat caagaagcac ataaatgttg cagcttcggt catgtccgga aatctcgggg
     1441 atgagaagac ctttgtgttc aagatatgcg atggatcaac ttcaattccc tgctatgcgg
     1501 taatgccggc ataccatttg agtgaaaaca acaaggtgct aattgacgca ttttatgggg
     1561 atatagtcga gatatatggc actcttgtac gtgagaacaa agaacaatct tctattaatc
     1621 ttttagtcac cagaaatact attgttaatt ttgcaggtta gtcctgcagc aatttcaaaa
     1681 tttttaatcc ctgctcaatg cattccatct tatccctggt ccttccaagg tctgcttctg
     1741 tttcaagact tattgccagc tctctgactt tatttatgat catattattg atagctgatg
     1801 tatcgaatga tagcggctct gccctgacaa tcctgttttg gtccgctttt ttatgtccta
     1861 tttgcggttt ctctgttttt cctgtttctt gcggctcgtt tttttgcttt tcttcctgga
     1921 aatcacacac aggacacaca attttcccct gatacctaaa cattggagcc ccgcactcat
     1981 gatgtgttgc aagcatagtt cctccttttt caaggagttt tgttattttc tggatcttat
     2041 catctgaatc tttttcgacc ataatatcac cgaaagtctt ttcaagcaat aatcctaatt
     2101 acagtttttg tttcatattt ataccccgtg gtgataactg tgtcggaaaa tcctagcgaa
     2161 gtcataaaac agtgtactga agttctggaa aggatcgtcg gagatgattc ggttccaaga
     2221 aatattagac gttcagcaga taacataaaa aatgtactat caaatgaaaa gcagtcgttg
     2281 tcaaaaagag cggctatggt gatttcgaat cttgatgaca tggggaacga cccaaatatc
     2341 ccgattcata cgagaacatt gatctgggga ttatccagca aactggaatc aatcccggtt
     2401 gatcgatgat tcataatttt tcttgagata tttccagcgt taacctaccc tgttgtttcc
     2461 actggaaatt attgttttta aaattgcgtt ttgttttaat tttgccgtat tttttttctt
     2521 attttaaatt ttttattggt cttattttaa aatctttgtc taattattat ttgtttaatt
     2581 atttttaaac tttattttta taataagcta aaacagaaat atttatatat agttagttag
     2641 taactgagtc acatcggatt tttaaaaatt taaaaaaaaa gaataacatc gttttttaaa
     2701 aaatctccac aagaaataat ggggtaggtt atcctgtttt ccaatggaaa tgatggggtg
     2761 tagaagaaaa agaacctttg tatctcttga acaggaacaa tgtcattttg aatgaaccga
     2821 tatacagaga tatgtttccc atcatttttt tcctctattg atcatcaagt ccaattgaaa
     2881 tagaggggta tttaccataa gtattaagcc ctatccttcc attggagtgt tctaggaggc
     2941 tgaaggatgg agacaaaaac gtcatcaata aatggtattt tcgaagacat gttgatgcgt
     3001 gaaagtattt tcgagaatag agaggtgcta aggtcaacat atacacctga ttatctgccg
     3061 cacagggacg agcaggtaaa aggattagct actatattgg tttcagcgct aagaggcgaa
     3121 accccttcta atatattgat atatggaaag accggtaccg gtaaaaccgc tgtggctaaa
     3181 catgtaggga aggaacttga gaaaaccagt gaaatgcata gtgttccatg cgttgtggtt
     3241 tatattaact gtgaagttgt ggatacacag tatcgtttgc ttgcatcaat tgcacggcat
     3301 ttcgaaaaag acattcccat gactggctgg ccaaccgacc aggtttatac ggaactaaaa
     3361 agcgcacttg attcggataa acgtatagcg ataataattt tggatgaggt ggataaactt
     3421 gtatgtaaag gagacgatgt gctttacaat cttacccgta taaattctga cttaaaaaaa
     3481 gctaaagtca gtcttatcgg agttacaaac gatcttaaat tcacggaatt cctggaccca
     3541 agggtaaaaa gttcattggg agaagaagag ataatttttc ccccgtacga tgccaaccag
     3601 ataagggata tccttaacca gagagcacat atggctttca agccccaggt actggaagaa
     3661 acggtaattc ctctttgtgc cgcttacgca gcccaggaac atggagatgc aaggcgggca
     3721 ctggaccttt tacgtgtatc cggagaaatc gcagaacgaa taaaatcgaa taaagtcctt
     3781 gaggcgcacg ttaagcaggc tcaggagaag atcgagatag acaggatagt ggaggtagta
     3841 aggactttgc caagccagtc taaacttgtt ttattgagcg ttgttcttct gggatataac
     3901 ggtgaaagta atctcacgac aggtgaagcc tataatattt acaaacagat gtgcaggatg
     3961 ataggaattg aaatcctgac ccagcgaagg gttacagatc tgatttccga gcttgatatg
     4021 atgggcatcc tgaatgcaac tgttgtgagt aagggaaggt acggaagaac caaagaaatc
     4081 tctttgagcg tcccttctga aagcacaaaa agagttctat ttgaggatta caggttaaaa
     4141 ccgctggaag atttcaggcc tcccaaccag acaaaattgt acgcttgaaa tcatttttct
     4201 tatttaatcg gatgccccac agctctgctg tgggagcttc acttaaagca tgcaagattc
     4261 cctcttggaa taagtgaaat acatccaaga aatggtaccc tggtaaatat tgcttttccg
     4321 atcacccatt cttttttgac aggctgcatg taacttatac ttccctgctg gtcatatgca
     4381 gcattggttt tgctgttatc accttttgta atgtaacctg catacggagc aggaggtcct
     4441 cccgaccaca taggatctcc attgtccaca taatacatgg cgcgatgaat aattggtgta
     4501 gcttccactc ttccaagcgg tttgtataag atgacatcgc catattcgtt aaaggatgaa
     4561 taatccaatt tttttccggc ttcatttgtg attatttccg ttcgatcggc acttttaaca
     4621 aatatgatat cacctatctg aatgttaggg atcatacttc cgctttctac agctaccatg
     4681 ggtgtatata gtcccaatgc gatcttcgaa accgaggcaa aaataattac aacggtaagt
     4741 acaaaaagaa aatcccgcaa
//
LOCUS       DATGBN010000109         8199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_280126, whole genome shotgun sequence.
ACCESSION   DATGBN010000109 DATGBN010000000
VERSION     DATGBN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280126"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VKL21_02415"
     CDS             <1..761
                     /locus_tag="VKL21_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HMB44653.1"
                     /translation="QVRLAFATAIQTDPEILMMDEVLAVGDMEFQQKCLDVFQRYVRE
                     KKTIIFVSHDLNSVRRFCNRSVLLRHGEQVAFGKTNEIIDKYVYGGTERHTEDPELKV
                     EGSKGKVKEKKTRWGDKKVEIIEVKFIDKFKRENTTFYSGDPVTIQIKYNVNESIDDI
                     VIGIALYSDTDMLCYGTNTDIKEFNVDRTMGNKEINLQIINLTMMEGRFHLTVAVHSM
                     KHIPYDWLDKQFSFNVIKKGNNAGLFEIPCAWEQ"
     gene            802..2256
                     /locus_tag="VKL21_02420"
     CDS             802..2256
                     /locus_tag="VKL21_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMB44654.1"
                     /translation="MDTFEIKDDEINVEEIMHQIRENIQKRRQSGTYDTEIESLINQP
                     LQIPLARTEDGDIKSDLDYLNSHWDVHAEYSIISHRPVIGQLLISGRQLIHGEVKRYA
                     DLIAGKQTEFNLRVIRSLKNILDIFETKIASVDNRIDTVDNRIASVDNRIDTVDNRMD
                     TVDNRMDTVDNRIDTVDNRIASVDNRIDTVDNRIDTVDNKITEVKGDTERRVTASVNK
                     IISAANNDIKNKAWLSNILEERVKKDLSNFNNNSQNDEIFNYFLFEEKYRGSTEDIKK
                     RQTIFLDYFKNGQNVLDIGCGRGEFLSLLKENGIGAKGVDINEDMVLYCKKNGLDVEK
                     VEALSFLRSIADKSLDGIFSGQVIEHLQPEDLINLVKICHDKLKYGTYFIAETINPLC
                     LSVFAKSFYMDMSHVKPVHPATIKFLLESVGFREVEFRFFSPHTEDSKLGKVMITDNM
                     NGEEKARIEVMNRNIDMLNELLFGYQDYAVVGKK"
     gene            2264..3433
                     /locus_tag="VKL21_02425"
     CDS             2264..3433
                     /locus_tag="VKL21_02425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB44655.1"
                     /translation="MKIAFVIPWYGDIPGGAENECKRTAENLQKSGIEVEILTTCVKE
                     FLSDWNNNFYKEGVYEVNGITVRRFRLRQRDTKLFDRINLKLMHNQKVSPHEEVKYIT
                     EMINSDNLYKYISENTSKYDYFLFIPYMFGTTYFGSKICPERSILIPCLHDESYAYMG
                     IYKKMFEGVRGIIFHSDLELKLAEKLYDLKCNKRVLGEGIDTEIQFDRKRFQKKNGIN
                     DEFLLYAGRREIGKNVPMLVDYFIKYKKENDNNLRLILIGNGEVNIPRSQNKNIIDLG
                     FVPIQDKYDAYSAATVLCQPSVNESFSIVIMESWLCGTPVLVHANCEVTRDHCIKSNG
                     GLYFNNYEEFKECINFYRMNPHLARKMGNNGRDYVINNYAWNKVVEKYKTFLLEL"
     gene            3448..4482
                     /locus_tag="VKL21_02430"
     CDS             3448..4482
                     /locus_tag="VKL21_02430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB44656.1"
                     /translation="MTANFSTEDAIGNYIQTLDRIFQKSGFNTRIFSDITFQNPHSSK
                     YIPTGKDILWFHYSIYSDNLKYLEKSKDIKIMDFHGVTPYHLFRGYNAQLEQLCKKGS
                     EMLKVFSDHVDLCIVHSDYSRYILEENGYKKIIKLPLVVDLSRLSKIKDDETLAPLLN
                     KIKYMLFVGRIVPQKSIIDIVKVFYHLKQFQPNFKLFLVGDYGISKEYTFEIMKLIKQ
                     FNLYDDVVLTGKLDDAGLTTIYKYANIFIVLSEWETFCVPLIEAMYYQVPSIGYNRTA
                     IPETIGDAGILVDKIDHMEIARLINELLNDHRRYELLKLKCKERANNFTEAQLELQLP
                     GILKEVLEKK"
     gene            4536..5681
                     /locus_tag="VKL21_02435"
     CDS             4536..5681
                     /locus_tag="VKL21_02435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB44657.1"
                     /translation="MEVNGGSELHCRWIVEHLSRYYDVEVITTCAKDYMTWEDEYEQG
                     IDIVNGITIRRFRVDAPRDVHKFNRFSEKIFGNKHTADEELEWMKLQGPYSTELLNFI
                     KNNENSYDFFIFFTYLYCTTFYGLPLVKDKAILVPTAHDEPPIYLSIFDGLFKMPKAI
                     VYNTEEERKFINSKFNNTDIPGETVGVGIEMPEHIDADDFKRKFDVDKFIIYVGRIDE
                     SKGCKELFEYFIRFKKEIKSNIKLVLLGKPMMKIPNHTEIISLGFVSEQDKFNGIKAS
                     ELQLMPSKYESLSMVILETWLCSNVVLVNGNCEVLKGHCIKSNGGLYYTNYEEFKECL
                     NLLLSNSQLRAKMGENGQKYVEENYRWEKIEIKYQQLMINLNAMSKE"
     gene            5718..6782
                     /locus_tag="VKL21_02440"
     CDS             5718..6782
                     /locus_tag="VKL21_02440"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMB44658.1"
                     /translation="MKILITGGAGFVGSNLAIEFKKNRKDVEIIAFDNLKRRGSELNI
                     SRLKTKGIEFIHGDIRNKEDFEALGNSDVVIECSAEPSVMAGVDSSPEYLLNTNLLGT
                     VNCLEYVRKSGADVIFLSSSRVYPVRTINNLKFVEKPTRFELSDDQDVPGVSSNGITE
                     EFSLNGTRTLYGATKLASELLLQEYIETYGLRGVINRCGVLTGSWQMGKVDQGVVVLW
                     VAKHLFNQSLSYIGYGGKGKQVRDILHVKDLYRLLEIQINNMDSHNGEIYNVGGGLER
                     TISLLELTALCQKYTGHKISIERVNEDRAGDIRIYITDNSKISKKTGWKPEITIDQII
                     PEISGWIEKNSKHLRLILCI"
     gene            6797..7861
                     /locus_tag="VKL21_02445"
     CDS             6797..7861
                     /locus_tag="VKL21_02445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMB44659.1"
                     /translation="MNIALITGSAGLIGAESVRFFSKKGFKVVGIDNDMRKVFFGDEA
                     STQWSRKKLESDIRDYIHYNEDIRDRTAMEAIFEKYNKDIKLIIHTAAQPSHDWAAKD
                     PFTDFTVNANGTLTLLEMTRQYCPEAVFIFTSTNKVYGNTPNDLPLVELETRWEIDIS
                     HPYYEKGIDESMSVDQTKHSLFGASKLAADVLVQEYGRYFNMKTVCYRGGCLTGPGHS
                     PTILHGFLAYLMKCALIGTHYTIFGYKGKQVRDNIHSYDLVNAFWHFYQKPRMGEVYN
                     MGGGRHSNCSMLEAIAMCEEITGKKMEYSYTEDNRIGDHIWWISDVSKFKSHYPQWDY
                     KYDLKDILIEIYQNSVFSKI"
     gene            7897..>8199
                     /locus_tag="VKL21_02450"
     CDS             7897..>8199
                     /locus_tag="VKL21_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB44660.1"
                     /translation="MNKISIVVPAHNEEDNLNHLMDELIPVLERHRETEDYEIVIVND
                     NSSDRTPKIIDELAAGNSRIKAVHRSSDPGFGSAIKEGFKNATGDIIIPVMGDLSDD"
BASE COUNT         2882 a         1224 c         1794 g         2299 t
ORIGIN      
        1 tgcaggtgag gcttgctttt gcgacagcga tacagacaga tcctgagata ctgatgatgg
       61 atgaggtgct ggcggttggg gatatggaat tccagcagaa gtgcctggat gtgttccaga
      121 ggtatgtcag ggagaagaag actataatat ttgtttctca tgatttgaat tcagtgaggc
      181 ggttctgcaa caggtcggtt ttattaagac atggcgagca ggtggcattc gggaagacga
      241 atgagatcat tgataagtat gtgtatggtg ggacggaaag acacactgag gaccctgagc
      301 taaaagtaga ggggtcaaag ggaaaagtaa aggagaagaa gacacgatgg ggggataaaa
      361 aagttgagat aatagaggtc aagttcatcg ataaatttaa gcgggaaaac acgacgtttt
      421 actccggaga tccggttacg attcaaataa aatataacgt aaatgaatcc atcgatgata
      481 ttgtaattgg gatagccctt tattcggata ctgacatgtt gtgttatggc accaatacag
      541 atattaaaga atttaatgtt gataggacca tgggaaataa ggagatcaat ctgcagataa
      601 ttaatttaac aatgatggaa ggtaggttcc atttgacagt tgccgtgcac agtatgaaac
      661 atattccata tgactggctc gataagcaat tttcattcaa tgtgatcaag aaaggaaata
      721 atgctggtct atttgaaatt ccatgtgcat gggaacaatg aatttattta tgaatagcaa
      781 gaacaatggt gaacaataat aatggataca tttgaaataa aagatgacga gatcaatgta
      841 gaagagataa tgcaccagat ccgggagaat atccagaaaa ggagacaaag cggcacatat
      901 gatacagaaa ttgaatcgtt gattaatcag cccttacaaa tacccctggc cagaacagaa
      961 gatggcgata taaaatctga tcttgattat ttgaattccc actgggatgt gcatgctgaa
     1021 tacagtataa tttcgcaccg gccggtaatt ggccaattgc taatatcggg aagacaattg
     1081 atacacgggg aagtgaaacg ctatgcagat ctcatagctg gaaaacagac cgaatttaat
     1141 ttgagggtta ttcgttcatt aaaaaatata cttgatattt ttgagacaaa gatagcttct
     1201 gtggataaca ggatagatac tgtggataac aggatagctt ctgtggataa caggatagat
     1261 actgttgata acaggatgga tactgtggat aacaggatgg atactgtgga taacaggata
     1321 gatactgtgg ataacaggat agcttctgtt gataacagga tagatactgt ggataacagg
     1381 atagatactg tagataataa gataactgag gtcaagggag acaccgaaag gagggtaact
     1441 gccagcgtaa ataaaataat ttccgctgca aataatgata ttaaaaacaa ggcatggctt
     1501 tcaaatatac tcgaagagag ggttaaaaag gatttatcaa attttaacaa taattcacaa
     1561 aacgatgaaa tattcaatta ttttttattc gaagaaaaat atcggggaag tactgaagat
     1621 ataaagaaac gccaaactat ctttttagac tatttcaaga atggtcagaa tgtgcttgat
     1681 atagggtgcg ggcgcggaga gtttttatca cttttaaaag agaacggaat tggcgcaaaa
     1741 ggcgttgaca taaatgagga tatggtactt tattgcaaaa agaatgggct ggatgtggaa
     1801 aaagttgaag cactgtcttt tcttcgatca attgcagata aatcactgga cgggatattt
     1861 tccggacaag ttatcgaaca tctgcagccg gaggatttga taaatcttgt aaaaatatgc
     1921 catgataaat tgaaatatgg gacttatttt attgcagaaa ctattaatcc tttatgcctt
     1981 tctgttttcg cgaaaagttt ttacatggat atgtcccatg ttaagcccgt gcatccggct
     2041 acgataaagt tcctgttgga atcagtaggt tttagagaag ttgagtttag atttttctca
     2101 ccacacacag aggatagcaa acttggcaag gttatgataa ctgataatat gaatggtgag
     2161 gaaaaggccc gtattgaggt aatgaaccgc aacatagata tgttgaatga attattgttc
     2221 ggatatcagg attatgctgt tgttggaaag aaatagggga cagatgaaaa tagcttttgt
     2281 aataccgtgg tacggggata ttccgggtgg ggcagaaaat gaatgtaaac gaactgctga
     2341 aaatctccaa aagagcggta ttgaagttga aattctcact acatgcgtaa aagaattcct
     2401 gagcgattgg aataataatt tttataagga aggggtttac gaggtaaatg gaataacggt
     2461 caggaggttc agactaagac aaagagacac aaaattgttt gacaggataa accttaaact
     2521 aatgcacaat caaaaagttt cacctcatga agaagttaaa tatatcactg aaatgattaa
     2581 cagcgataat ttatacaaat atatatctga aaatacatca aaatatgatt attttctttt
     2641 cataccatat atgttcggga ctacttattt tgggtccaaa atatgtcctg aaagatctat
     2701 tttaattccc tgtcttcatg atgaaagtta tgcatacatg ggaatttata agaaaatgtt
     2761 tgaaggggtt agagggatta tttttcattc agatctggaa ttgaaactgg ctgaaaaatt
     2821 atatgatttg aagtgtaata agcgagtact tggagaaggt atcgacacag agatacaatt
     2881 cgacagaaaa agattccaga aaaaaaatgg aatcaatgat gaatttttgc tatatgccgg
     2941 aaggcgagag attgggaaga atgttcctat gctggttgat tattttatta aatataaaaa
     3001 agaaaatgat aataatttga gactgatttt aatagggaac ggtgaagtga atataccaag
     3061 aagccaaaat aaaaacatca ttgatctggg ttttgtccca attcaagaca aatatgatgc
     3121 atattctgct gcaactgtat tatgccagcc atcagtgaat gagagttttt cgatcgtaat
     3181 aatggagtcc tggctgtgtg gaacacccgt gcttgtccat gcgaattgtg aggtcaccag
     3241 agatcattgt atcaaaagta atggtggact ctattttaac aattatgagg agtttaaaga
     3301 gtgcataaat ttttatcgaa tgaatccgca tttagccaga aaaatgggaa ataacggaag
     3361 agattatgtc attaataatt atgcctggaa caaagtggtg gaaaaatata agactttttt
     3421 actggagtta taatgcagat ttttcaaatg actgcaaatt tctcaacaga agatgccatc
     3481 ggaaattata tccagacatt ggacaggata tttcaaaaat ccggttttaa taccagaata
     3541 ttttctgata tcacgtttca gaatccccac tcttcaaaat atatacccac aggtaaagac
     3601 attttatggt ttcattattc aatatactcc gataatctga agtatctgga aaaaagcaag
     3661 gatataaaaa tcatggattt tcatggtgta accccttacc atcttttcag aggatataat
     3721 gcacaactcg aacagctctg taaaaaaggc agtgaaatgt taaaagtgtt ttcagatcat
     3781 gtcgatttat gcatagtgca ttctgattat tcaagatata ttttggaaga aaatggctat
     3841 aaaaaaatta taaaattacc ccttgtcgtg gatcttagca gactttcgaa aataaaggat
     3901 gatgaaacgc ttgcacctct actaaacaaa ataaaataca tgctttttgt gggaagaatt
     3961 gttccacaga aatcaattat tgacattgtt aaagtatttt accatttgaa acaattccag
     4021 ccaaatttca aattattcct tgtgggggat tatggtattt cgaaggaata tacttttgaa
     4081 ataatgaagc tgataaaaca attcaacctg tatgatgacg ttgtcttaac cggaaaactt
     4141 gatgatgcag gattaaccac aatatataaa tatgctaata tatttattgt tttatcagaa
     4201 tgggagacat tttgtgtgcc tttgatagag gcaatgtatt atcaagttcc ttccatagga
     4261 tacaatagaa cagcaattcc tgaaacaatt ggagatgctg gaatacttgt tgataaaatt
     4321 gaccatatgg aaatagccag actaataaat gagcttctga atgatcacag aagatatgag
     4381 ttattgaagc taaaatgcaa agaaagagct aataatttca cagaggcaca attggaactt
     4441 caattacccg gtatattaaa agaagttctt gagaaaaaat gaaaaccgag aaaaataaga
     4501 gattgaaaat agcctttgtt gtccagcgat acggattgga agtaaatgga ggatctgaac
     4561 tccactgtag atggatcgtt gaacacctgt ccagatatta cgatgttgaa gtaatcacaa
     4621 cctgtgctaa agattatatg acctgggaag atgaatatga acagggcata gatattgtca
     4681 acgggattac tattagaaga ttccgtgtag atgctccaag agatgttcat aaattcaata
     4741 gattttcaga gaagatcttc gggaataagc acaccgctga tgaggagctt gaatggatga
     4801 aactgcaggg tccatattcc actgaattat tgaattttat aaaaaataat gagaattcct
     4861 atgatttttt tatttttttc acatatcttt attgcacaac cttttatgga ctgcccttag
     4921 tcaaagacaa agcaatatta gtacctacag ctcatgatga accaccaatt tatttgtcta
     4981 tcttcgatgg acttttcaaa atgcctaaag caattgtcta caacacagaa gaagaaagga
     5041 agttcattaa ctcgaaattc aataatactg acataccggg agagacagtt ggagtcggca
     5101 ttgaaatgcc tgagcatata gacgctgatg atttcaagcg aaaatttgat gttgacaaat
     5161 ttatcattta cgttgggcgg atagatgaat ccaaaggatg taaggaatta ttcgagtatt
     5221 ttattcgatt caaaaaagag ataaaatcaa atatcaagct cgttttattg gggaagccta
     5281 tgatgaagat tccaaatcac acagagatta tttccttagg ttttgtttct gagcaggata
     5341 aattcaatgg aataaaagca tctgaattac aattaatgcc ctcaaagtat gaaagcctgt
     5401 ccatggtaat attagagaca tggctttgca gcaatgtagt gcttgtgaat ggtaattgtg
     5461 aggtgctgaa agggcattgt atcaaaagta atggagggct gtattataca aattatgaag
     5521 agtttaaaga atgtttaaat ctgctgctaa gtaattcaca acttcgagcg aaaatgggcg
     5581 aaaatggaca gaagtatgtt gaggagaatt ataggtggga aaagattgaa ataaaatatc
     5641 agcaactcat gattaattta aatgccatga gcaaagaata aaatactttc ccatatgtta
     5701 taaccattac aaaataaatg aaaatattaa taacaggcgg cgcggggttt gtaggttcta
     5761 atctagctat tgaattcaag aaaaatagaa aagacgttga gataattgct tttgataatc
     5821 tcaaaagaag aggttcagaa ctgaacattt cgagattaaa aactaaaggc attgaattta
     5881 tacacggcga tataaggaat aaggaagact ttgaggccct gggtaattct gatgtagtga
     5941 ttgaatgttc agcggaacca tccgttatgg caggagttga ttcttcgcca gaatatctgt
     6001 tgaacaccaa ccttctggga actgtaaatt gccttgaata tgtaagaaag agtggcgctg
     6061 atgttatatt tttatcatca agcagggtat atcctgtaag aaccatcaat aatttgaaat
     6121 ttgttgaaaa accgacacgg tttgaactat cagatgatca ggatgtgcct ggtgtttctt
     6181 caaatggcat aactgaagaa ttctcattaa acgggacacg cacattatac ggcgccacaa
     6241 agctggcatc ggaattgctt ttgcaggagt atattgaaac atacgggcta agaggtgtta
     6301 tcaatcgctg cggagttttg acaggaagct ggcagatggg gaaagtggat cagggcgtag
     6361 ttgtgctatg ggtcgcgaaa catttattca atcaatcatt aagctacatc ggatatggcg
     6421 gcaaaggaaa gcaggtgagg gatattctcc atgtaaaaga cctgtatcgt cttcttgaga
     6481 tccagatcaa taatatggat tcgcataatg gtgaaatata caatgtagga ggcgggcttg
     6541 agcgaacgat atcccttctt gaattgaccg ctctgtgtca gaaatataca gggcataaga
     6601 tatcgataga aagggttaat gaagatcgag cgggcgatat acggatatat ataacagaca
     6661 acagcaagat ttccaaaaaa acaggatgga agcctgaaat cacgatcgat caaattattc
     6721 cggaaatatc tggctggatc gaaaaaaatt ccaaacattt aagacttatc ctttgcattt
     6781 aaagcaggaa ataaaaatga atatagctct gatcacaggc tcagcgggtt tgataggcgc
     6841 tgaatctgtc aggtttttca gtaaaaaagg gtttaaagtt gtcggtatag acaatgatat
     6901 gagaaaagtc tttttcggtg atgaggcgtc aacgcaatgg agccggaaaa aacttgaatc
     6961 tgatataagg gattatatac attacaatga ggatattcgt gatagaacgg ctatggaagc
     7021 catttttgaa aaatataata aggatataaa attaataata catacagcag cgcagccatc
     7081 tcatgattgg gctgcaaaag atcctttcac agactttact gtgaatgcta acggaactct
     7141 cactcttctt gaaatgacaa ggcaatactg tccggaagca gttttcatat ttacttccac
     7201 caataaagta tatggtaata ctccaaacga tttgccactt gtcgaacttg agaccaggtg
     7261 ggagatcgat atatcccatc cctactatga aaaaggtatc gatgaatcaa tgagcgttga
     7321 ccagacaaag catagcctgt tcggagcttc gaaactggcc gcagatgtgc ttgtccagga
     7381 gtatggcagg tatttcaata tgaaaaccgt ttgctacaga ggggggtgcc tgacaggacc
     7441 gggacattcg ccaactatct tgcatggatt cctggcatat ctcatgaaat gcgccctcat
     7501 cgggactcat tatacaatat ttgggtataa aggaaagcag gtcagggata atatccatag
     7561 ttatgacctt gtgaatgctt tctggcactt ttatcaaaag ccgcgaatgg gtgaagttta
     7621 taatatggga ggcggcaggc attccaactg ctcgatgctg gaagcaatcg caatgtgtga
     7681 agagatcaca ggcaaaaaaa tggagtattc atatactgaa gataaccgga tcggcgacca
     7741 tatctggtgg ataagcgatg tgagtaaatt caaatcgcat tatccacaat gggattataa
     7801 atatgaccta aaagacatat tgatcgagat ctaccagaat agcgtttttt caaaaattta
     7861 atgaattatt aaaaagtgaa gagaattggc taaaaaatga ataaaatctc catcgtagtc
     7921 cctgcgcata atgaagaaga taatctgaac catttaatgg atgaacttat tcctgttctt
     7981 gaaaggcata gagaaactga ggattatgaa atcgttatcg taaatgataa cagttctgac
     8041 cggacgccga aaataatcga tgagcttgcc gccgggaact caaggataaa agcagttcac
     8101 aggagctctg accccggatt tggaagtgct attaaagagg gattcaaaaa tgccacagga
     8161 gatataataa tacctgtaat gggcgacctt tcagatgac
//
LOCUS       DATGBN010000110         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_241475, whole genome shotgun sequence.
ACCESSION   DATGBN010000110 DATGBN010000000
VERSION     DATGBN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241475"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VKL21_02455"
     CDS             <1..660
                     /locus_tag="VKL21_02455"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44661.1"
                     /translation="PVFCITPKECGRIVSTFTETSIREVLDIDEEAFRRNELSITIKG
                     QAVVPVSPGVQNGKAVSESVDAKLEIASRIFDDFQAEGKDVLYIIGPGTTTMEVKKKL
                     GIDGTLLGVDAFRNGKTFWKDASEKRLLDALLEFKGKVVIIVSPIGSQGFIFGRGNQQ
                     IGPEVIKRADEIKVIASPEKLEFTPDLHVDTGDNELDEKLRGYIKVLSGYHDFKFMKV
                     V"
     gene            956..1237
                     /locus_tag="VKL21_02460"
     CDS             956..1237
                     /locus_tag="VKL21_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44662.1"
                     /translation="MKSQARQKIKEGKVVKVQIEYDEVIETIRITGDFFLHPEDILDK
                     IEKGIIGLEKTVSEERIISNINRTIAGEDIQMIGISPESIAGLVKEALK"
     gene            1234..1980
                     /locus_tag="VKL21_02465"
     CDS             1234..1980
                     /locus_tag="VKL21_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HMB44663.1"
                     /translation="MRWRVVDLETHDAYFNMALDEAISEGITNGSSPPTIRFYTWKPG
                     AVSIGYFQSIKDEVNLETCQELGIDRIRRWTGGGAVYHDTEGEITYSVIAPASSFPKN
                     IQESYQVICSWIVNGLRKLGINAVFSPVNDILVDNKKISGSAQTRRNGILLQHGTVLY
                     DLDLATMFSVLNVSKHKITDKMIKDAKERVTCISKYANVDKKDVYEALVGAFTNDKDH
                     EYGTWSKKEIERAQELADQKYRTEVWLSMR"
BASE COUNT          670 a          347 c          495 g          492 t
ORIGIN      
        1 cccgtttttt gcattacgcc aaaagaatgt ggaagaatag tatccacatt tactgaaaca
       61 tcaattagag aagtacttga tatcgatgag gaggcttttc gcaggaatga attaagtatt
      121 acgataaaag gccaggctgt agtgcctgtc agtccgggcg tccagaacgg aaaagctgta
      181 tcagaatcgg tggacgctaa attggagatc gcatcccgga tatttgacga tttccaggcc
      241 gaaggaaaag atgtgcttta tataattgga ccaggcacca cgacgatgga agtaaaaaaa
      301 aagctgggta ttgatggaac actgctgggt gtagatgcat tccgcaacgg gaagacattt
      361 tggaaagatg catccgaaaa acgtcttctt gatgctcttt tggaattcaa gggaaaggtg
      421 gtgatcattg taagtcccat aggaagccag ggatttatat tcggaagggg aaaccagcag
      481 atcggtccgg aagtcattaa acgcgcagat gagatcaaag tgatcgcttc cccggaaaaa
      541 ctcgaattca cccctgatct acatgtcgat accggggaca atgagcttga tgaaaaatta
      601 aggggatata ttaaagtttt aagcggatat catgatttca aattcatgaa ggtcgtttag
      661 gacgtggggt agaagggtag agaatccccg cgacctctgt cgggggatgt cgtaccccct
      721 ttcattgtaa tacctgcccc aaaaaagcaa ttgttgaagt tgtattttga gggataggca
      781 taacgatata ttattaaaac catatataga ttgttgaaat tgtaccgtag gaactacggg
      841 acttcaagcc cgaccgagta atctcaatag agaaccaatg aagcgggaag ctcccctttt
      901 taagggagta gttcacaata taagttgcat ttaaaatata agatataaat gaattatgaa
      961 atcccaggcc aggcagaaaa tcaaagaagg aaaagttgtc aaagtgcaaa ttgaatacga
     1021 tgaagtaata gaaacgatca ggataacggg tgattttttc ctgcatcccg aagatatcct
     1081 ggataaaata gaaaaaggta tcatcggtct tgaaaaaacc gttagtgaag agcgtattat
     1141 ttcaaatatt aatagaacca tcgcaggcga agatatccag atgataggaa ttagtccgga
     1201 atccattgcc ggattggtaa aggaggcgct gaaatgagat ggagagttgt ggatcttgaa
     1261 acccacgatg cttattttaa catggctctt gatgaagcaa taagtgaagg aattacgaat
     1321 ggttcatccc cacccactat caggttctat acatggaaac caggggctgt atctataggt
     1381 tacttccaga gtatcaaaga tgaagtcaac ctggagacat gccaggaact gggaattgat
     1441 cgtatcagga gatggaccgg cggtggggca gtatatcatg atacggaagg tgagataacc
     1501 tatagtgtaa tagcgcctgc cagctcattc ccaaagaata tccaggagtc atatcaggtg
     1561 atctgcagct ggatagtgaa cggtcttaga aaactgggta ttaacgcggt tttcagcccg
     1621 gttaatgaca ttctcgtgga caataaaaaa atatccggca gtgcccagac caggcgcaat
     1681 gggatactgc tccagcatgg aactgtgctt tacgatcttg atcttgcaac catgttcagt
     1741 gtattgaacg taagtaaaca taagatcact gacaagatga taaaggatgc aaaagaacgg
     1801 gtcacctgta tttcaaagta tgcaaatgtg gataagaaag atgtttatga ggccctggtc
     1861 ggggctttca caaatgacaa agaccatgaa tatggcacat ggagcaagaa agagatagaa
     1921 agggcacagg agttagctga tcaaaaatac aggacagaag tgtggttatc tatgagataa
     1981 aaatttgtaa attatgataa aacc
//
LOCUS       DATGBN010000111         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_474423, whole genome shotgun sequence.
ACCESSION   DATGBN010000111 DATGBN010000000
VERSION     DATGBN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474423"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VKL21_02470"
     CDS             complement(<1..759)
                     /locus_tag="VKL21_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44664.1"
                     /translation="MREKKGLNNIINKVLPGLSRLVSVLGISLLFIQFLWVPALGQKV
                     EYHNGYDLIYNDDGSKDIINGDFNFLRWDGVWRPNSELNISNGSWPYLYSGNATTADF
                     KVNDTTLSIPKANTQFKLKKNSISYNLVYSKSELLGKETSINLTDALIDFPYNLKSKK
                     PKKEYRDQFNIRYDRFHFKAGMEHIVIHDDTPVDYIEDGKVYQDVTYLFPKDYDFKID
                     NNGQIRLKFKKNALDKLKGNIIIEIRTWDVVGANN"
     gene            1292..3307
                     /gene="metG"
                     /locus_tag="VKL21_02475"
     CDS             1292..3307
                     /gene="metG"
                     /locus_tag="VKL21_02475"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMB44665.1"
                     /translation="MSNFPSDKPVLVTCGLPYANGKCHIGHLRTYVPGDIFVRALRKQ
                     GQEVTFVCGSDAHGTPIVVNAEELKITPTELVNKYHKHFDSIFKAMEVEFNIFGNTDS
                     LTSHSRTESIVKALIDRGYVYPQVIRLAYCPHDERFLPDRYVEGICPYCGKPARGDEC
                     DQGCGKHLEPGEIKSAKCRICGNPAQYREQEHFFFKLSSFGSFLSDYLKTLGGTSNAI
                     NYAKEWAKELKDWCITRNLEWGVRFPGHDDLVVYVWVDAPIGYISFTEEWANNTGGDW
                     EKYWRGDSRIIHFIGEDITYHHCIFWPAMLKGAGYTPPWAVVASGMLKIDDKKFSKSR
                     GYVVWVDEDYLDHGFHPDLLRYYIANYTSHTKELNFSWKVFQEKVNNELVGALGNFLH
                     RTQHFAQKNFGNVPDGRVDQEILEKIKATIETVIMAFDEYEFKKATDAMMELAQYGNA
                     YFQSFEPWALIKKDKEACARVLKNCLQIGKALILLFEPILPGNMEKAWKQLGLSGDVH
                     KAMFEDALIEIPAGELRVPEILFTKIDDKKIKEMEAILDKRIKIAMSKVKKHEAKEEK
                     TEITYEEFQELDIRIGKVLSAENIKGSDKLLKLEVDIGEKRQIVAGIAQSHNASGLVG
                     RQVVVIANMKPAKLFGVESRGMVLAADSDGAVLLIPEKEVKEGTKVK"
     gene            complement(3443..>3795)
                     /locus_tag="VKL21_02480"
     CDS             complement(3443..>3795)
                     /locus_tag="VKL21_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033214.1"
                     /note="with 7S RNA and Srp19 binds to the signal sequence
                     of presecretory protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein Srp19"
                     /protein_id="HMB44666.1"
                     /translation="MYKQLEAVNKMGPLKQVMSMIPLGKLGMKVSDDMFAVTQEKMKK
                     YKYIMDSMTDKELEEPKHINSPRITRIARGSGTKYDEVRELLKYHKMMQKTMKGMSGG
                     GKFNIQKMMKKFGM"
BASE COUNT         1113 a          719 c          815 g         1148 t
ORIGIN      
        1 attattggcg ccaactacat cccatgttct gatctctata atgatatttc cctttaattt
       61 atcgagtgca tttttcttaa attttaatcg aatttggccg ttattgtcta tcttgaaatc
      121 gtaatctttc gggaaaagat atgttacatc ctggtatact tttccatcct caatatagtc
      181 cacaggtgtg tcgtcatgta tgactatatg ttccatccct gccttgaaat ggaacctatc
      241 atatctaata ttaaattgat ccctgtattc ttttttaggt ttctttgatt tcaaattata
      301 aggaaaatct ataagtgcgt ccgttagatt gattgaagtt tctttcccaa gcaattctga
      361 ttttgaatat accagattat aactgatcga attttttttc aatttgaatt gtgtatttgc
      421 ctttggaata ctcagggtag tatcatttac cttgaaatca gcagtcgttg catttcctga
      481 atataagtaa ggccagctcc cattggaaat atttaattcg ctatttggtc tccagacacc
      541 atcccatcga agaaaattaa aatctccatt tataatatcc tttgagccat cgtcattata
      601 gatcagatca tatccattgt gatattccac tttttgccca agggcaggga cccacaggaa
      661 ttgaataaat aaaagactta ttccaagtac agataccagt cttgaaagac caggcaatac
      721 tttatttatg atattattta gacctttttt ttctcgcata ttcataccca tttttattta
      781 ggttgtcaat ggagcagatt aatatataaa tttttgtata ggtttgacat tataattatt
      841 gctataatta ttataatgat tttatcttaa aaaagtatcg gtaaataagt tccatgcata
      901 atggttccca ttgccgccat gataatttaa ttgcttgaaa atcaaagggt ttttcctgaa
      961 aaatacctgc aatcagcata agaacactgc ttgttcctgt ggctgtgttc gcatctaagt
     1021 cttgaaacag ggatattgcc tgttgcgata atcttattat ctataatgtg tatgtagcgt
     1081 tgcttccccc ttgccaaggt ggcggaacgg ccacgcaatc gcctgcagag cgattccaat
     1141 ccggttcgac tccggacctt ggcttctttt ctttcaaatt attgcataat tttttttagt
     1201 caggataatg ggcatttttt gaaaaaagga atatttgcat aggcaatcaa ccacagaaac
     1261 atattttata aagaaatccc atgtagcatc catgtcaaac tttccatctg ataaacctgt
     1321 ccttgttacc tgcgggcttc cgtatgctaa tggcaaatgc catatcgggc acctgcgtac
     1381 ctatgttccg ggagatatct ttgtgagagc tctgagaaaa caggggcagg aagttacatt
     1441 cgtatgcggg tcggatgccc atggtactcc gatcgttgta aatgcagagg aattaaagat
     1501 aacccccaca gaacttgtca ataaatacca caagcatttc gatagtattt tcaaagcgat
     1561 ggaagtggaa ttcaatatat tcggaaatac cgattctctg accagtcatt caaggactga
     1621 aagcattgtg aaggcactga tcgacagggg gtatgtttat cctcaggtta tacggcttgc
     1681 atactgcccg catgatgaac gattccttcc ggacaggtat gtagagggaa tctgtcctta
     1741 ttgcggcaag ccggcacggg gcgatgaatg cgaccaggga tgcggaaaac atcttgaacc
     1801 cggggagata aagagcgcaa aatgccggat atgcgggaat cctgcccaat accgtgagca
     1861 ggaacatttc tttttcaagc tgtcatcttt tggaagtttt ctctcggatt acctgaaaac
     1921 attaggcggc acatccaatg ccatcaatta tgcaaaagag tgggcaaagg aattgaagga
     1981 ctggtgtatc acgagaaatc ttgaatgggg ggttcggttc cccggtcatg acgaccttgt
     2041 tgtatatgta tgggtggatg cgcctatagg atacatttca tttacagagg aatgggctaa
     2101 taacacaggc ggcgactggg agaaatactg gcgcggtgac tcgcgcatta ttcatttcat
     2161 aggtgaggac atcacctacc atcattgtat cttctggccg gcgatgctaa aaggcgcagg
     2221 ctacacaccg ccatgggctg tggtcgcatc aggcatgctg aaaatcgatg ataagaaatt
     2281 ctccaagagc cggggttatg ttgtgtgggt agatgaggat tatctggatc atggtttcca
     2341 tcctgacctt ttgcggtatt atattgcgaa ttacacttcg catacaaaag aattgaactt
     2401 ctcatggaaa gtattccagg aaaaggtcaa taatgaactg gtgggagcgc ttggaaattt
     2461 tttgcacaga acacagcatt ttgcccaaaa gaatttcggg aatgtcccgg atggcagagt
     2521 agaccaggaa attcttgaaa agataaaggc cacgattgaa accgtaatta tggcttttga
     2581 tgaatatgaa ttcaagaaag caacagatgc tatgatggaa cttgcgcagt atggtaatgc
     2641 atatttccag tcgtttgaac cctgggcgct cataaagaaa gataaagaag catgtgcccg
     2701 tgttttaaag aactgccttc agatcggaaa agcgcttata ttgttatttg aaccgatcct
     2761 gcctgggaat atggaaaagg catggaaaca acttggactt tccggggatg tccataaagc
     2821 aatgtttgaa gatgccctta tcgagatacc tgccggagaa ttaagagttc ctgagatatt
     2881 attcacaaaa attgatgata agaagatcaa ggaaatggaa gctatccttg ataaaaggat
     2941 aaagatagca atgtcaaaag tgaaaaagca cgaggcgaag gaagagaaaa ctgaaataac
     3001 atatgaagag ttccaggaac ttgatataag gatcgggaaa gtattatctg cagagaatat
     3061 caagggttcg gataaactgc tcaaattaga agtcgatatt ggcgagaagc gccagattgt
     3121 tgcagggatt gcacaatccc ataatgcttc cgggcttgta gggaggcagg ttgttgtgat
     3181 cgctaacatg aagccggcaa aactcttcgg agttgagtca aggggaatgg tgcttgcggc
     3241 tgatagtgat ggtgccgtat tgctgatacc ggaaaaagaa gtgaaagaag gcactaaggt
     3301 taagtgactt tattataaaa tggagataaa gggggcgttg cgaaacatcg gaggggatgg
     3361 atgcgatgcc gctgccccaa tactaatata aatctcttta cttataaata ttttccataa
     3421 tcatatcatc agaaaaaaac aatcacattc cgaatttctt catcattttc tgaatgttga
     3481 actttccacc gccgctcata cctttcattg tcttttgcat cattttatgg tatttcagga
     3541 gttcgcgaac ttcatcatat ttggtccctg accctcgcgc aatacgtgtg atcctgggac
     3601 tgttaatatg ttttggttct tcaagttcct tatctgtcat cgaatccata atatacttgt
     3661 attttttcat tttttcctgg gtaacagcga acatgtcatc cgagactttc attcccaatt
     3721 tcccaagcgg gatcatggac atgacctgtt tgagcggtcc cattttattt acggcttcca
     3781 gctgtttgta catat
//
LOCUS       DATGBN010000112         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_125335, whole genome shotgun sequence.
ACCESSION   DATGBN010000112 DATGBN010000000
VERSION     DATGBN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125335"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VKL21_02485"
     CDS             <1..802
                     /locus_tag="VKL21_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMB44667.1"
                     /translation="RINTLLTNRQTVNDAVLNLADGRVFERDFIPVYFEEVFVGHMWQ
                     YRDITERKKTEKQIEASLHEKETLLKEIHHRVKNNMQIVSSLLDHQIQFIKDKNVIEI
                     FAESQNRIASMALVHEKLYQSKDLAKIDFSDYISDLTANLFQSYQENSGKIRLNMNIE
                     NIQLDIDFAIPCGLIINELVTNSLKYAFPADRVGEIKIVFRRTIENTLELVISDNGIG
                     IPLDLDFRKTGSMGLHLVTILAENQLHGKINLNRNEGTEFIIEFKGIK"
     gene            799..1650
                     /locus_tag="VKL21_02490"
     CDS             799..1650
                     /locus_tag="VKL21_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB44668.1"
                     /translation="MTQEKTVKQILIVEDEIIIAEGLQRKLKKMGYDVPVIVSSGEET
                     IKMIKENNPDLVLMDIVIHGKMDGIETAGQIHSLFDIPVVYLTAFADEKTLERAKITE
                     PFGYLIKPFKERELQITIEIALYKHEMERKLKQSERSLSEKNQWLAAVIESIGDAVIA
                     TDPEGTIRLMNPLAEALTGWKQNEALGKPLTGIFNIISEETNKETENPVTKAIREEKF
                     YGLADHTILVTKEGIKIPVDIIGSTIKSDGDSVIGIVLIFCDIFERTRIYEALKISKS
                     LLLRKTR"
     gene            1647..>2179
                     /locus_tag="VKL21_02495"
     CDS             1647..>2179
                     /locus_tag="VKL21_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMB44669.1"
                     /translation="MSDYIIGIKELDKEIEGIRKGSNIMLIGPPMSGKELILNFIMYH
                     GAAINENAVITVTTRESTLHILEWFKENELNLPLDRIGIIDCVSKTLGGAVKEDENIR
                     IASSPVDLTGIGVKISYFFEEFITKKNIRKNQLHMNSLSTILMYSNIQTVFRFLHVFT
                     GRIKSIGGLGIYLIESG"
BASE COUNT          789 a          349 c          467 g          574 t
ORIGIN      
        1 aaggataaac actttgctca ccaatcgtca gacggtgaat gatgcggtgt tgaaccttgc
       61 tgatgggcgc gtctttgagc gcgatttcat acccgtttat tttgaggagg tgttcgtggg
      121 gcatatgtgg caatatcgcg acataactga gcgcaagaag actgagaagc aaattgaagc
      181 ctctctgcat gagaaagaaa cgcttttgaa agaaattcac catcgtgtaa agaacaatat
      241 gcaaattgtt tcaagtctcc ttgatcatca aatccagttt atcaaagata aaaacgtgat
      301 tgaaatattt gcagaaagcc agaacaggat agcatctatg gctcttgtgc atgaaaaact
      361 ttatcaatca aaagacctgg caaaaatcga cttctccgat tacatcagtg atttaacagc
      421 taaccttttt cagtcttatc aggaaaattc cggcaaaata agacttaaca tgaatatcga
      481 aaacattcaa ctggatattg attttgccat tccatgcgga ttgataatca atgaactggt
      541 cacaaattct ttaaaatatg catttccagc agatagagtt ggtgaaatta agatagtgtt
      601 tcgcagaaca attgaaaata cgcttgaact tgtgataagt gataatggca ttggcatacc
      661 tctggatctg gattttagaa aaaccgggtc aatgggttta cacctggtca caatactggc
      721 agaaaaccag cttcatggga aaatcaatct aaatcgaaat gaaggaacag aatttataat
      781 tgaatttaaa gggataaaat gacacaagag aagacagtga aacagatact gatcgttgaa
      841 gatgaaatta ttattgctga aggcttacaa cgaaaattga agaagatggg atatgatgtt
      901 ccggtaatag tatcttcagg agaagaaact attaaaatga taaaagaaaa taatcctgat
      961 ctcgtgctga tggatattgt aatacatggt aagatggacg ggattgaaac agcaggacaa
     1021 atacattctc tttttgacat tccagtggtt tacctaaccg cttttgctga tgaaaaaaca
     1081 ctggaaaggg caaaaattac agaaccattc ggatatttaa ttaaaccatt caaagaaaga
     1141 gaactacaaa taacaattga gatcgccctc tacaaacatg aaatggaacg aaagttaaaa
     1201 cagagtgaaa gaagtttgag tgaaaagaac caatggcttg cagcagtaat tgaaagtata
     1261 ggcgacgctg tgatagccac ggacccggaa ggaactataa gacttatgaa cccgctcgca
     1321 gaggcattga cgggctggaa acagaatgag gctttaggga aacccctgac aggcatcttc
     1381 aatattatta gtgaagaaac gaacaaagaa actgaaaatc ctgtaacaaa ggcaatccgg
     1441 gaagaaaaat tctatggtct tgctgatcat acaatattgg taacaaagga aggtataaaa
     1501 attcctgttg atattatcgg ttcaacaatc aagagtgacg gggatagcgt cattggtata
     1561 gtactgattt tttgcgatat ttttgagcgc acaaggattt atgaagcgtt gaaaatatca
     1621 aaatcattat tgttgaggaa gacgagatga gtgactatat aattggaatt aaagaactgg
     1681 ataaggaaat cgagggtata agaaaaggtt cgaatattat gctgatcggc cctcccatga
     1741 gtggaaagga gttaatcctt aattttatta tgtatcatgg agcagcgata aatgagaatg
     1801 cagtaataac tgtaacaacc cgcgaatcaa cccttcatat cttggaatgg ttcaaagaaa
     1861 atgaattgaa tttgcctttg gaccgtatcg gtataatcga ctgcgtaagc aaaacccttg
     1921 gcggtgcggt caaagaggat gaaaatatta gaatagcgag cagtcctgtt gatctgacag
     1981 gaatcggggt gaaaataagc tatttttttg aagaattcat aacgaaaaag aatatacgga
     2041 aaaaccaact ccacatgaat tcactttcaa caattttaat gtattcaaat atccagacag
     2101 ttttcaggtt cctccatgtt tttacaggac ggataaaatc aatcggaggt cttggaatat
     2161 atctgataga aagcggcat
//
LOCUS       DATGBN010000113         1907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_86372, whole genome shotgun sequence.
ACCESSION   DATGBN010000113 DATGBN010000000
VERSION     DATGBN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86372"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VKL21_02500"
     CDS             <1..615
                     /locus_tag="VKL21_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMB44670.1"
                     /translation="RAISRQISLCVFSKTEDKEALSKASHDFLLGIESLSPDLLRFIR
                     NENYIPIEGFLLIFYSAHRLFSSEAEGMITMPRLRNIAKIYYLVRELGKDEKLKNLFD
                     YRFHTDYGFSYSDRLFEDIQLLVAMGMVDEDLRYFENKGRWQQRYEYVLTSDGLLYAE
                     SIASAYQNELVIIKNYLTVNKHSIPRDIVSVASKRYGRIIKAHR"
     gene            complement(830..1072)
                     /locus_tag="VKL21_02505"
     CDS             complement(830..1072)
                     /locus_tag="VKL21_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44671.1"
                     /translation="MSECFLCNTALEPVNDSELEGVDMCVDCCENMDTVIRDYISSSP
                     GGFDLVLDIIADEISNPESKLRDALKDVVKEVISEK"
     gene            1219..1569
                     /locus_tag="VKL21_02510"
     CDS             1219..1569
                     /locus_tag="VKL21_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44672.1"
                     /translation="MDEKALYARHTLNFKLVKLSWALFLILIGGSWILESLGKIDNSQ
                     KWGIIYAGCGVILLLLNLMRIAWKINTSKFTTWLGTLGLLFGIATFYKFNFSIWAAAI
                     LILGLIMLLEVFRK"
     gene            1613..>1907
                     /locus_tag="VKL21_02515"
     CDS             1613..>1907
                     /locus_tag="VKL21_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HMB44673.1"
                     /translation="MILLTGATGFIGSRVLQALTMKNLKVRCLVRKQKTSQNPNITYV
                     IGDVLDSDSLISATAGVETVYYFIHMMGNQPKGEQKKFDVLDRIAIENMVEACK"
BASE COUNT          603 a          360 c          387 g          557 t
ORIGIN      
        1 agagccatct cgcgccagat ttccctgtgc gtattctcaa agacagaaga taaagaagca
       61 ctttcaaagg cgtcgcatga tttcctcctg ggcattgaga gtctttcccc tgaccttttg
      121 cgttttataa ggaatgaaaa ttacattccg atcgaagggt ttcttcttat cttttacagc
      181 gcccacagat tgttcagcag cgaggctgag ggaatgatca ctatgccaag gcttcgaaat
      241 attgcaaaga tctattatct cgtaagagag cttggaaaag atgaaaaatt gaagaattta
      301 tttgattaca ggttccatac cgattatggc ttttcataca gcgataggct tttcgaggat
      361 atccagctcc ttgttgcaat gggaatggta gatgaagacc tgcgctattt tgaaaacaaa
      421 gggagatggc agcagcgata tgaatatgtc cttacaagcg atggtctcct gtatgcagaa
      481 tcgatcgcat cggcatacca gaatgaactg gttattatta aaaattattt gaccgttaac
      541 aaacattcca tccccagaga tattgtaagc gtggcttcaa aaagatacgg caggataata
      601 aaagcccaca gataagctcc gaaatttttg gatgaattta tatacgataa aaattactaa
      661 taagaccata ggatgatgga aacatgaatg aaacaaatga caaaaaaagc gacattatgg
      721 ttattttggt tgttctaatt gtagtgctta ttatagcagg cgtcctgtac ttattctaca
      781 gagtgccaaa ttaaaaaaaa agaaaaaaag gtttaaattt atatattttt tatttctcag
      841 atattacttc ctttacaaca tctttaagcg catccctgag cttgctttca gggtttgaaa
      901 tttcatccgc tattatatca agtacaaggt caaacccgcc aggactggat gatatataat
      961 ctcttataac ggtatccata ttttcacagc agtccacgca catgtccacg ccttctaatt
     1021 cggagtcgtt aacaggttca agagcagtat tacacaggaa acattcactc atattatcac
     1081 cagcgccgtt aatgaatctg acatcatatc aatgttatgc atattgacac actccgtggc
     1141 taaagccggg agattcttgg ttcagtaaca ttaaagtttt agccgtttat tataatgcta
     1201 atgaaagtgg aagtcttaat ggatgaaaag gcactgtatg ccaggcatac gctcaacttc
     1261 aagcttgtga aactatcatg ggcgctattc ttgattctta ttggaggaag ctggatcctt
     1321 gaaagcctcg gaaaaataga taactcccaa aaatggggaa ttatttatgc aggatgcggc
     1381 gttatcctgc ttttgttgaa cctgatgagg atagcctgga agatcaatac cagtaagttc
     1441 actacatggc ttggcactct tggattatta ttcgggatag cgacatttta taagttcaat
     1501 ttctcaatat gggcggcagc gatactgatc cttggcctca taatgctttt ggaagtgttc
     1561 aggaaataac agatcaaatt aaatactcaa aaaccaatat taataatatt ctatgattct
     1621 actcacaggc gccacgggtt ttattggaag ccgtgtccta caggctttaa ccatgaagaa
     1681 cctgaaggta aggtgccttg tcaggaaaca aaagacttcc cagaatccga acatcactta
     1741 tgtcatcggt gatgtccttg atagcgattc acttatttct gctacagcag gggttgaaac
     1801 ggtttattat tttattcata tgatgggaaa ccagccaaaa ggtgaacaga aaaaattcga
     1861 tgttcttgac agaattgcca tcgagaacat ggtagaggcc tgcaaac
//
LOCUS       DATGBN010000114         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_8442, whole genome shotgun sequence.
ACCESSION   DATGBN010000114 DATGBN010000000
VERSION     DATGBN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8442"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1360
                     /locus_tag="VKL21_02520"
     CDS             212..1360
                     /locus_tag="VKL21_02520"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMB44674.1"
                     /translation="MLKLNKKICLCIAVMFLASVGTMTAAALNDTEQLGKNLFFDTNL
                     STPPGQACAACHGPEVGYTGPDPKINAHGAVYEGAVNGRFGNRKPPSAAYAGDSPLLH
                     YDSGQGTWIGGMFWDGRATGWTLGDPLAEQAMGPFLNPLEQNNLDAAAVITKVVTGPY
                     NDLYNDVCGSATDKYECIARAIAAYERSSEVSAFTSKYDAVQEGKATFTTKEANGLAL
                     FEGKGNCANCHVPPLFTDFTYDNLGVPRNLENPFYNELKYNPMGSAWIDEGLGGFLKS
                     IGYSPNATELGKMKVPTLRNVDKRPNNGFKKAYAHNGYFKSLEEIVHFYNTRDVPGAD
                     WNGKPWPSAEVPENVNTAELGNLGLTKQEEKNIVAFLGTLSDGYRSRP"
     gene            1774..3075
                     /locus_tag="VKL21_02525"
     CDS             1774..3075
                     /locus_tag="VKL21_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HMB44675.1"
                     /translation="MLTITENEPTPLLKDFAAFIDYLKTHKIILTEANEFISGKNLFE
                     LNRQMAHPVQDTSTRTAQIYYPLFHLFYHLVLGGKLFKKIPEKNRIVLKLTDRLQSYK
                     DLKPAEKYFFLLETFWTDINWAHLQPEDFGSSPFNFRRIDTILADIGGMTPGKKIRLR
                     EELRVSIYDLKYFFHYFSYFGFWEVTPYKISPKRNYPAHAFMAESITPTVFGVAIAPV
                     LNEARNLLEWNLPHRRKLGEYKPVPGSPVPGEEIYTLLAGNKKRKIPVSTTKVKKSKL
                     GDPFCLPFVSLFASGELQKTLPRGESKPMDGIFVFKVSLEKNIWRRIELSSDHMLLDL
                     HNSIQSAYGFGDDHLYSFFMDGKMQSDEVFTSPYDDTGPHVDVACIGDLGLTVGQKIL
                     YLFDYGDMWRFQVELEEIRPKGIKPLNPKIIESKGKSPKQY"
     gene            complement(3145..4098)
                     /locus_tag="VKL21_02530"
     CDS             complement(3145..4098)
                     /locus_tag="VKL21_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44676.1"
                     /translation="MDEIAEQAKAQLMMVEHIYDLKIINRDEVAGLIAANVDNNRQVL
                     TICTSLNSWILLNKKKGDAVIPHEVLSKLFETTRWQGSMHRQGNMLDLPKLMLGTSPF
                     IGAGQFGSKSLDYRRRFFENPDNMTRLFLHSASLGVRVVQLVGYRPLVEALMKAQELA
                     GDFFVAVTIPRGDFATNLDLISPLEPEFVSVHAQFCDQNDSRLNEWIDLIRDAGAKPA
                     ASTHSPGTTIPLLDDLDFEAYLAPVNSVGYGMEPDIESTLKALQRTKKAVIAIKPLAA
                     GKVSPERSVFEYIYRHADSIAVGITSEKEMEETYSAALAYG"
     gene            4212..>4483
                     /locus_tag="VKL21_02535"
     CDS             4212..>4483
                     /locus_tag="VKL21_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44677.1"
                     /translation="MPKKIEQYCQEHTNPESQLLHELVTETFAHTAFPEMEIGHLEGA
                     FLRILVRLLRAKRILELGTFTGYSSLLMAEGLPDDGKLITCDIDPET"
BASE COUNT         1459 a          838 c          926 g         1260 t
ORIGIN      
        1 agcccttaga gacaaaaggg ttatattcta atataacata ttcaatatgt agaaatgtgg
       61 ggaaaaaagt ttatcgaaaa aaaactgtaa tctgaaaaat gattttttaa taaaaattaa
      121 atgcagaaaa acgttagtta aaatatgaaa gctccttaat ttctaataaa aattgaaaat
      181 ctaaaatact tcaaagggag gaattaacta aatgttaaag ttaaataaga aaatatgtct
      241 ttgcatagct gttatgtttc ttgcttctgt aggaactatg actgcagcag cattaaatga
      301 tacagaacaa ttaggaaaga acttgttttt cgatacaaat ctatcgactc caccgggtca
      361 ggcgtgtgca gcatgtcatg ggcctgaagt aggatataca ggacctgatc caaagatcaa
      421 tgctcatggt gcagtttatg aaggtgcagt taatggaaga tttggaaacc gtaaaccacc
      481 atcagccgca tatgctggag atagtcccct tttgcattat gatagtggtc aaggaacatg
      541 gataggtgga atgttctggg atggtagagc aactggttgg acgcttggag atccacttgc
      601 agaacaggct atgggcccat tcctcaatcc attagaacag aacaatcttg atgctgccgc
      661 agttattaca aaagttgtga ctgggcctta taacgaccta tacaatgatg tttgtggaag
      721 tgcaacagat aaatacgaat gtattgccag agccattgca gcatatgaga gatcttcaga
      781 agtaagtgct ttcacatcca aatacgatgc cgtccaagaa ggcaaagcta cttttacaac
      841 aaaagaggca aatggtttag cattgttcga aggaaaaggt aactgtgcga actgtcatgt
      901 acccccattg ttcacagact tcacatatga caatttggga gtaccaagaa atttagaaaa
      961 cccgttctat aacgaactta aatataatcc aatgggatct gcatggatcg atgaaggatt
     1021 gggaggtttc ctcaaaagta ttggttattc accaaatgca acagagttgg ggaagatgaa
     1081 agttccaact ttgcgtaatg tcgacaaaag acccaataat ggtttcaaaa aggcctatgc
     1141 acacaacggc tacttcaagt ctctggaaga aattgtacat ttctataaca caagagatgt
     1201 gcccggggca gattggaacg gaaaaccatg gccatcagca gaagtaccag aaaatgtcaa
     1261 tacagctgaa ttaggtaatc ttggactgac taaacaagaa gaaaaaaaca tagtcgcctt
     1321 tttgggaacc ctatcagatg ggtataggtc caggccataa agaaacaaaa tttaaaatgt
     1381 gagcgatata atcgctcatt ttttttactc tttgataaac tattgctgat actgcacctt
     1441 taaatcctca attttttacg ttacaattct gcccataaaa taccgcagga tctcttgatc
     1501 ataacccaat tatctataat tattgtagta tttgtgattg aaacatggat atgttctaca
     1561 aaaaatagaa ttgatctgag gaaagaactt gagtttctgc aaacgtgccg atctgagatc
     1621 atgttatgta gagatgcgga aatcacaact tatttcggag acgttataaa tcggatcata
     1681 ggcgggatgg gaatgtgaag gagataagag aataactata atgtattcat aaaaaggtat
     1741 aaacacaaga aataaatcaa attgagagat tacatgctaa ccataactga aaatgaaccc
     1801 acaccgctct tgaaggattt tgccgcattt atcgattacc tgaaaacaca taaaatcatc
     1861 ctgacagaag ccaatgaatt catatctgga aaaaatcttt ttgaattgaa ccggcagatg
     1921 gcgcatcctg tacaggatac ttctactcgc acagcacaga tatattatcc acttttccat
     1981 ttgttttatc atctggttct ggggggaaaa ttgttcaaga aaattccgga aaaaaacagg
     2041 atagttttaa agctaacaga tcgtcttcaa tcatacaaag atttaaaacc agcagaaaaa
     2101 tattttttcc ttcttgagac tttctggact gacattaact gggcgcattt gcagccagaa
     2161 gactttggat cttcaccttt caattttaga agaatcgata ctattttagc cgatattggt
     2221 ggaatgacgc caggaaagaa aattcgatta agagaagagt taagggtttc gatttatgat
     2281 ctgaaatatt ttttccacta cttttcatat tttggtttct gggaagttac tccttataag
     2341 atatctccaa aacggaatta tccagctcat gcttttatgg cagaatcaat cacacctacg
     2401 gtttttgggg ttgcgattgc cccggttttg aatgaagcaa ggaatctatt ggaatggaat
     2461 ttgcctcatc gtcgaaaact gggtgaatat aaacctgtac cgggatcgcc tgtaccaggg
     2521 gaagaaatat atacgctttt agccggaaat aagaaaagga aaataccagt atcaaccacc
     2581 aaagttaaga aaagtaaact cggagatcca ttctgcctac cttttgtatc tctgtttgca
     2641 agtggagaat tgcagaagac acttccccgc ggcgaatcaa agccgatgga tggaatattt
     2701 gttttcaaag tgtctctgga aaaaaacatc tggcggcgta ttgagttgtc ttctgaccat
     2761 atgctacttg atcttcataa ttcgattcaa agcgcatatg gttttggcga tgatcatctg
     2821 tattcttttt ttatggatgg aaaaatgcag tctgacgaag tattcacatc cccatatgat
     2881 gatacaggtc ctcatgttga tgttgcttgt attggtgacc ttgggctgac ggtcgggcag
     2941 aaaatacttt atcttttcga ttacggagac atgtggcgat ttcaggtaga actggaggaa
     3001 atacgaccca agggcattaa acctcttaat ccaaagatta ttgaatcaaa aggaaaatct
     3061 ccgaaacaat attgatcaat aagaaatatc cttcaagcaa tagatatgaa aatcgataac
     3121 acagaagcca aaaaaataag aattttatcc atatgcaaga gcggctgagt aagtttcctc
     3181 catctctttt tctgaagtga tcccgaccgc aattgaatct gcatgcctgt agatatattc
     3241 gaaaacagat ctttctggag agacttttcc tgcagcaaga ggcttgatcg caataaccgc
     3301 cttctttgtt cgttgaagag cctttagtgt gctttcaata tctggttcca ttccataacc
     3361 gacagaattc accggggcaa gatatgcctc aaaatccaag tcatcaagca agggaatcgt
     3421 agtgcctggt gaatgtgtag aagctgcagg ttttgctcct gcatccctga tcagatcgat
     3481 ccactcattc agccgggaat cattctggtc acaaaactga gcatgcacag aaacaaactc
     3541 aggctcaagc ggtgaaataa gatcaagatt tgttgcaaaa tctcctctgg gaatagtgac
     3601 tgcaacgaag aaatcaccgg cgagctcttg cgctttcatt aatgcctcta caagaggtcg
     3661 ataaccaaca agctggacaa ccctgactcc caggctggct gaatgaagaa aaagtcttgt
     3721 catgttatca ggattctcga aaaaccgcct gcggtaatca agagacttag agccgaactg
     3781 acctgcgcct atgaagggtg aggttcccag catcaatttt ggcaaatcta gcatattgcc
     3841 ttgtctatgc atgcttccct gccatctggt ggtttcaaaa agcttcgata gtacctcatg
     3901 cggtatgact gcgtcccctt ttttcttatt gagaagaatc caagagttca gcgaggtgca
     3961 tatagttaag acctgtctgt tgttatccac gttcgcagca atgagtccag caacctcatc
     4021 gcggtttatg attttcaaat catagatatg ctcgaccatc atcaactgtg cctttgcttg
     4081 ctcggcgatt tcatccatgg tgatatattt ctggcatttg tggtttaaag cttttcaatg
     4141 aaaaaatata gcaacaataa ggggctaata tttagttacc tttgatgata ttattaagta
     4201 tgaagagaat tgtgccaaaa aaaatcgagc aatattgcca ggaacacaca aatccggagt
     4261 cacagcttct tcatgaactt gtaactgaga cttttgcaca tacagctttt ccagaaatgg
     4321 aaataggaca tctggaggga gcatttttgc ggatactggt gaggttactt cgagcaaaac
     4381 gaatccttga attaggaaca ttcactgggt acagttcgct tttaatggca gaaggattac
     4441 cagatgatgg gaagctgatt acctgcgata ttgaccctga gac
//
LOCUS       DATGBN010000115         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_474615, whole genome shotgun sequence.
ACCESSION   DATGBN010000115 DATGBN010000000
VERSION     DATGBN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474615"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="VKL21_02540"
     CDS             complement(<1..1867)
                     /locus_tag="VKL21_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMB44678.1"
                     /translation="MNTGKSKANRLIYEKSPYLLQHAYNPVNWYPWGEEAFEKAKKED
                     KPIFLSIGYSTCHWCHVMEKESFEDDEVSSLMNDVFVSIKVDREERPDIDSIYMALCQ
                     AMTGSGGWPLNLILTQDKRPFHALTYIPKESRFGRVGMLDLIPQVKQLWNSKRDMVED
                     AAYRNLKALEVTGSAAGEKPGVDILHQAYEQILGMFDEEHGGFGHAPKFPASHNLMFL
                     LRYWKRTGDKMALMMVEKTLSSMRNGGIYDHIGFGFHRYSVDPGWIVPHFEKMLYDQA
                     MLAMAYTEAYQATGKEEYKKTCSEIITYVMRYMTSPDGGFYSGEDADSEGEEGKFYLW
                     TKNEIRKVLGDDENELINKIFNIKEKGNFPEGNGRNIFYLSKPVPEISDFSAANIEKR
                     IESSRGKLFTARENRIHPGKDDKILTDWNGLMIAALTKASQAFDEPGYSKAAKKAADF
                     ILSKMRNEMSVLYHRYRDGEAAISGFLDDYSFFIWGLLELYEATFEIHYLKAAIELNE
                     DLLMHFWDNGAGGFFITSEDAGNILIRKKDIYDGAVPSGNSVAMLNLIRLSKIKADPE
                     LERKAEAIGQAFSRVIEMAPAGYAMFMSSLDFALGPSDEIVIAGDLQAEDTKDMLK"
BASE COUNT          545 a          500 c          369 g          627 t
ORIGIN      
        1 cctttagcat atcctttgta tcttctgcct gcaaatcccc tgcaataaca atttcatccg
       61 aaggccccaa agcaaaatca agcgacgaca tgaacatagc ataaccggct ggcgccatct
      121 caattacccg tgagaatgcc tgcccgatag cttctgcttt gcgttcaagt tcaggatcag
      181 ccttgatctt gctaagccgg atcaggttca acattgctac agagttgcct gatgggactg
      241 ccccatcgta tatgtctttt ttcctgatca ggatatttcc ggcatcctcg gatgtaatga
      301 aaaacccacc agccccatta tcccagaaat gcataagcag atcttcatta agttcaatag
      361 ctgctttcaa ataatggatc tcgaaggttg cctcataaag ttccagtaat ccccatataa
      421 agaaagaata atcatccaaa aaaccggaaa ttgcagcctc cccatccctg taacggtggt
      481 aaagcacgct catttcgtta cgcattttgg aaagaataaa atctgcggct tttttagcgg
      541 cttttgaata tccgggttca tcaaaagcct gggaagcctt agtcagggct gctatcatca
      601 atccgttcca gtctgtcagt atcttgtcat cttttccggg atgaatacgg ttttcacgag
      661 cggtgaataa tttacctcgt gacgattcaa tcctcttttc tatgtttgca gctgaaaaat
      721 cagatatttc aggaacaggt tttgataaat aaaagatatt tcgtccattt ccctccggga
      781 aatttccttt ttctttgata ttgaaaatct tattgatcaa ttcattctca tcatcaccga
      841 gaaccttgcg tatttcattt ttagtccaga ggtaaaattt cccttcttca ccctcactgt
      901 cagcatcttc accggaataa aatcccccat caggtgaggt catatacctc attacgtagg
      961 taataatttc actgcatgtt tttttatact cttctttccc cgttgcctgg taagcttcgg
     1021 tataggccat tgccagcatc gcctggtcat aaagcatttt ttcaaaatgg ggtacaatcc
     1081 atcccggatc aaccgaataa cggtgaaaac caaatccaat gtggtcatat atcccgccat
     1141 tacgcatgga tgaaagtgtt ttttcaacca tcatgagggc catcttatct cccgttcgct
     1201 tccagtagcg aagaaggaac ataaggttgt gagatgcagg aaatttaggc gcatgaccaa
     1261 aacctccatg ctcttcatcg aacatcccca ggatctgttc ataagcttga tgcagtatat
     1321 caacaccagg tttttcacct gcggctgatc cggtaacttc aagagctttt aaattccggt
     1381 aggctgcatc ttcaaccata tccctcttcg agttccagag ttgttttacc tggggtataa
     1441 ggtccaacat cccaacccgg ccgaacctgc tttctttcgg gatatatgtc aaagcgtgaa
     1501 agggtctctt gtcttgcgtc agtatcaaat taagcggcca tcctccgctt cctgtcattg
     1561 cctggcataa tgccatatag atgctgtcaa tgtcaggtct ttcttccctg tcaactttga
     1621 tacttacaaa gacatcattc atcaaagagc taacttcatc atcctcaaag gattcctttt
     1681 ccataacatg gcaccagtgg catgtggaat atccgatcga gagaaaaata ggtttatctt
     1741 ctttttttgc cttttcaaat gcttcctcac cccatggata ccaatttaca ggattatatg
     1801 catgctgtag aaggtacgga cttttttcat agatcaacct gttggctttt gatttccctg
     1861 tattcatttt cccggcctca taagcctgaa aaagcccgga aaattaccca ttgatctttc
     1921 tttttcaggc ggaaatatac cttcacttgc agacctgacc tcctctatag agtaaaacgc
     1981 cctgggatta aaccttttaa tcattcctat aattttttga aggtctcggc gcttaatgat
     2041 a
//
LOCUS       DATGBN010000116         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_241817, whole genome shotgun sequence.
ACCESSION   DATGBN010000116 DATGBN010000000
VERSION     DATGBN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241817"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VKL21_02545"
     CDS             complement(<1..1559)
                     /locus_tag="VKL21_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HMB44679.1"
                     /translation="MRIGVYICHCGSNIAGTIDVEGVRKHASELKDVVVARDLQFACG
                     DSGQEQVKKDILEEKLDRVVMAACSPRLHEVTFRRVLEQSGLNKHLLEMVNIREQGSW
                     VHTNHNGLATQKAKDLVAMGVAKVALLSPLEKRTVPANKNVLVIGAGVAGIEAALTLA
                     NMGIKVHLVEREPTIGGKMALLNEVFPNNDCSLCVLAPKMSDVQNHTNITLYTNSEIT
                     GIRGRAGNYRITGEKKPRYIDPKKCKGCIDICAKVCPIDVPNQFDYGIGARKSIYIPF
                     AQAVPLVACIDEHCVGCDLCRLACPAEAVDFDQKTENFEFTAGAIIVATGYQPFDARR
                     KEEYGYGRYKNVITNLELERMLSAAGPTHGRVVSPSTGADIKSAAFILCVGSRDEQVG
                     NPYCSKVYCMASIKNAMKLAEKYPEALISIHYIDIRAGGEMYEEYYRRVQELGVSFVR
                     GRVAEVEESDGKTIIHYEDTLCGEKCSDTVDLVVLATGMEADKDSSRIGRMLNLSTRP
                     DRFFQSAHPKMR"
     gene            complement(1780..3741)
                     /gene="rqcH"
                     /locus_tag="VKL21_02550"
     CDS             complement(1780..3741)
                     /gene="rqcH"
                     /locus_tag="VKL21_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HMB44680.1"
                     /translation="MKQEMSSVDVAALVRELHPRLLDAKITKIYQHSQDEIRIGLHIF
                     KEGRTNLVIEAGKRFYLTKNPEIAPKFPQSFPMLLRKHLTGGRITEISQYDFDRIIEM
                     QVQRGEDKTTLVIELFSRGNIVLLDSQKRIILPLKSISFRDRKVRGGEPYELPQAQIS
                     PITVQEEQLKEMFEHSDADIVRTVATKMNLGGQYAEELCIRAGIEKNTPAKDITDLAS
                     LQNALRDVFKPLSTELKPQIILKDEIKIDVLPFPISLYEKNEKISFGTFNEALDEYFS
                     GEAKKKNEAIKVVKAAKDDKMGSYEYRLQKQILALSKFRDDEQKLVHKGELIYAHYQT
                     CDGILKVINNARDKGYSWDEIKIILKKSDIPQAKVLKSINPERGLINILLDNEEVELD
                     IRLPVTQNSQVYYDRSKKLSGKIKGALTAIEETKKLTEKKEAPRIRKKIQKQKQKWFE
                     QFRWFISSDRFLVIGGRDAQSNEDIVKKYLQKKDIFFHAHVSGSPAVVIKTEGKDVPE
                     TTLFEAAQFTVSYSGIWKSGQASGDAYWVLPEQISKTPESGEYVAKGAFVIRGKRNYF
                     KDVLLEAALGLELGEEKKLIGGPLSAVKKTSQFVMEVEPGEFNQNDISKKIYRMLNDK
                     FEDKKLIKTIASPDRIAMFLPPGGSRVKE"
     gene            complement(3824..>4009)
                     /locus_tag="VKL21_02555"
     CDS             complement(3824..>4009)
                     /locus_tag="VKL21_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMB44681.1"
                     /translation="KKLGDQRSIAYTLAQIGLLYSHMSKKEEAIESTKKALDIFEKIG
                     LENEARKAKDQIKKMSI"
BASE COUNT          911 a          970 c          821 g         1307 t
ORIGIN      
        1 cgcatctttg gatgggcgct ctggaaaaac ctgtccgggc gggtagatag gttcagcatc
       61 ctgccgatcc gggaagaatc cttatccgct tccatacctg tcgcaagcac aacaaggtca
      121 acggtatcac tgcatttctc accacagagc gtatcctcat aatggattat tgtcttaccg
      181 tctgattctt caacttctgc aaccctcccg cgcacgaatg aaactcccaa ttcctgtacc
      241 cgcctgtagt attcctcata catctcgcca cctgcccgta tgtcgatgta atgtatggag
      301 atcagggctt caggatactt ttcggcaagt ttcatggcat tttttatgga agccatgcag
      361 tacacctttg aacagtaggg atttcctacc tgttcatcac gcgagccgac acacaggata
      421 aaagctgcgc ttttaatatc tgcgcctgtt gagggcgata ccactctgcc atgcgtgggg
      481 ccggcagcgc ttaacatcct ttcaagttca agattcgtta ttacattttt ataccgccca
      541 tatccgtatt cttcttttcg tcttgcatcg aagggctggt atcctgtggc gacaattatt
      601 gctcctgcag tgaactcaaa attctcggtt ttctggtcaa aatccactgc ctcagcagga
      661 caggccagcc tgcaaaggtc gcagcccacg cagtgttcat caatgcaagc cacaaggggc
      721 acagcctggg caaaaggaat gtatatggat tttcgcgccc ctattccata atcgaactgg
      781 ttggggacgt caatagggca gacctttgcg cagatatcta tacagccttt gcattttttt
      841 ggatctatat atcgcggttt tttctcgccc gttatcctgt aatttcctgc tcttcctctt
      901 atccctgtta tttcagagtt tgtgtaaaga gtaatatttg tgtggttctg gacatccgac
      961 atcttggggg caagaacgca aagggaacag tcattattgg gaaaaacctc gtttagaagg
     1021 gccattttcc ctcctatagt gggttctctt tcaacgagat ggactttgat ccccatgttg
     1081 gcaagagtaa gagcagcctc gatccccgct actcccgcac ctattaccag gacattctta
     1141 tttgcgggaa cggttctctt ttcaaggggg gaaagaagcg cgacttttgc tactcccatg
     1201 gccacaaggt cttttgcttt ctgcgttgcc agtccattat gatttgtatg aacccaggag
     1261 ccctgttccc tgatatttac catctcaagc agatgcttat tgaggccgga ttgctcaagg
     1321 acgcgcctga atgtaacttc atgaaggcgc ggcgagcatg ccgccattac tacgcggtca
     1381 agcttttctt caagtatgtc cttttttacc tgctcctgtc ccgaatcccc gcatgcgaat
     1441 tgcaagtccc tggctaccac tacgtctttt agctccgagg catgttttct gaccccttca
     1501 acatcaatag tgcctgcaat atttgacccg caatggcaga tgtaaacacc gattcgcatt
     1561 cagttctcca gattcttaat gtatattcgt gatatataag ttttggtcaa gataaatcca
     1621 ttatgatgtt attattattc atcctttcgt atgcgacatt tctgttatgc aacatatttg
     1681 ttgcttgaag ctataagttt tttagagaaa aaggaacctt gatgaagttc gcacagtgct
     1741 aagtgctaag atttgggatg gagatattct gtttgttttt tattccttca ccctcgaccc
     1801 gcccgggggc aggaacatcg ctatcctgtc aggtgatgct atcgttttta taagcttttt
     1861 atcctcgaac ttatcattca gcatacggta gatcttcttt gaaatgtcat tctggttgaa
     1921 ctcgccaggc tcaacttcca ttacgaactg tgatgttttc ttcacggcgc ttaaaggacc
     1981 ccctatcaac tttttctcct ccccgagctc aagaccaagc gccgcctcca gcaatacatc
     2041 tttaaaataa tttcgctttc cgcgtattac aaaagcccct tttgccacat attcacccga
     2101 ttcaggggtc tttgatatct gctcaggcag cacccagtag gcatcaccgc ttgcctggcc
     2161 tgatttccag atacccgaat acgacactgt gaactgcgca gcctcgaaga gtgttgtttc
     2221 tgggacatcc tttccttcgg tttttattac aaccgcaggc gaacccgata catgggcatg
     2281 gaagaaaata tcttttttct gtaggtattt tttcacgata tcctcattac tctgggcatc
     2341 ccttcccccg ataacaagga acctgtctga tgaaatgaac cacctgaact gctcgaacca
     2401 cttttgtttt tgcttctgga tcttcttcct tatcctggga gcttcttttt tctcggttaa
     2461 tttcttagtt tcttctattg cagtaagtgc gccttttatc tttcccgaca atttttttga
     2521 cctgtcgtaa tatacctggg aattctgggt cacagggagc cggatatcga gttctacttc
     2581 ctcattatca agaagaatgt tgatcaggcc tctttccgga ttgatggatt ttaatacttt
     2641 tgcttgcggt atatccgatt ttttcaatat tatcttgatt tcatcccatg aataaccttt
     2701 gtctctggca ttattgatta cctttaagat cccatcgcat gtctggtagt gcgcatagat
     2761 cagttcccct ttgtgaacca gtttttgctc atcgtccctg aattttgaga gcgcaaggat
     2821 ctgtttctgg agccggtatt cgtacgaccc cattttatcg tcctttgcag cttttacaac
     2881 tttgattgct tcatttttct tttttgcttc accgctgaaa tattcatcaa gcgcctcatt
     2941 gaaagtgcca aaagatatct tctcattttt ctcgtacagg gaaataggga aaggaagtac
     3001 atcaattttt atctcatctt tcagtataat ttgtggtttc agttcagtgc ttagcggttt
     3061 aaaaacatct cgcaaagcat tttgaagtga agccaggtcc gttatatcct ttgcaggtgt
     3121 atttttttca atgcccgcgc gaatgcagag ttcttctgca tattgacctc ccaggttcat
     3181 ttttgtggcc accgttctaa cgatgtccgc gtcagaatgt tcgaacattt ctttcagctg
     3241 ctcttcttgc acagtaatgg gacttatctg cgcctgcgga agctcataag gctcaccccc
     3301 ccgaactttc ctgtccctga aacttatgga ttttaatggc agtattatcc gtttttggga
     3361 atccaataag acgatatttc ccctggaaaa cagttcgatc accagcgtgg tcttatcctc
     3421 gccccgctgg acttgcatct cgattatccg gtcaaaatca tactgcgaaa tttccgttat
     3481 cctgcccccg gtcaggtgtt tgcgaagaag catggggaat gactgtggga atttgggggc
     3541 tatctccggg tttttggtga ggtaaaagcg ctttcccgct tcaatgacaa gatttgtcct
     3601 tccttctttg aaaatatgca gtcctatcct tatctcatcc tgtgaatgct ggtatatctt
     3661 tgtgatcttg gcatcaagaa gacggggatg caattccctg accagcgctg caacatccac
     3721 gcttgacatt tcctgtttca tgtgaagggg ctatatgaag gggatgttaa tttaagctta
     3781 ctatcaaaaa tctgcggatt tttgatatct gttggggcaa taactatatg ctcattttct
     3841 ttatctggtc ttttgctttt ctggcttcat tttccagtcc gattttttca aatatatcga
     3901 gagctttttt agtagattcg attgcctctt cttttttact catatgggaa tatagtaaac
     3961 ctatctgtgc cagggtgtag gctatacttc gctggtcgcc gagtttttt
//
LOCUS       DATGBN010000117         3883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_474696, whole genome shotgun sequence.
ACCESSION   DATGBN010000117 DATGBN010000000
VERSION     DATGBN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474696"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..562
                     /locus_tag="VKL21_02560"
     CDS             368..562
                     /locus_tag="VKL21_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44682.1"
                     /translation="MILNVYGDDEALNSILEDLDYIVGVKILSRRDLEDGRGVLIIQA
                     MRYTKYAIIKLAKKYELGVS"
     gene            complement(962..2071)
                     /locus_tag="VKL21_02565"
     CDS             complement(962..2071)
                     /locus_tag="VKL21_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMB44683.1"
                     /translation="MLLEHWIYSTAIAIIAGMIHFKKTGRDYSWIVIASALAPDLDMF
                     TGYIFNKFDIGILINGNPLKHGDFHNILVLLLFAGFVGLLLKMTGLKFKDSFIFAGIG
                     FSAHFFEDALVYNPAYAFLWPLSKHVFGIGIIYYQPDWYGIANTEVLLAGIIAMILCG
                     TVRTLYEGNSGLGRTMKAFAVGGIFLVMTIPMFEYYDGYLKDEVGGGNIVDRWQFTQN
                     ASWDSTVSHNGSHSAKIEISGNKSKKSGLWRSNKIPAKPNTTYTFSSWSKIEGAGGNN
                     SPAVRVVELDTNGKWMRQTNLVFSKGTHNWTKKQTKTKTNNNTRWVYVYANIWDGYGT
                     FWFDDVELYEEGANHNIIPNSGFERGVKKNINFVI"
     gene            complement(2377..3408)
                     /locus_tag="VKL21_02570"
     CDS             complement(2377..3408)
                     /locus_tag="VKL21_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44684.1"
                     /translation="MLKSSKAKVLPTPLEVVDYNFKNPVIRLGQYGRTSLNLKPDQDI
                     YIYEDLDNMQLVLCVYELKRSMYHSIICFSDIEYFKKLGVALSQRRINILSINFYKRD
                     SKYRATLLYEVPDYIRSKWEKNSLETILKKIPENDTEDKIKVLDTIEYSEDEKKDEGV
                     KHIVVPLIQYQDIFKKSLKSVKISKNWTIYYKISDDFKERANLKGADACKCIQIVELD
                     VGMLILKFYETNIFRINIETLNLPGSFVRLINICKHLNIEIEAIDLQKISLEEAGSAE
                     IIYILKFPDEIKNNVDNIKIKELFDEADTVLKEEDPEHGEIVKKHDKILERTAKKDKS
                     SFIHKIEIL"
BASE COUNT         1183 a          795 c          634 g         1271 t
ORIGIN      
        1 atatggttca aagttgcaga gcaatgctct ctgtaaccgt atttagccaa tcacgactta
       61 tatctgtatt tatgcaccca gcactgtcta aaaaactgaa atcgccttaa aacgcctttc
      121 aggcaaaata gaaactttta aaggcgtttt cccaaaatat cagaaaacgt aaataatatg
      181 tgtgaatccg ggaaaatacg tgtccgccga ctttccaacg gaggaatacg tgtgctccga
      241 ttccttttcg ttctccctct tcctccctcc tccgctcgta ctgttttcat atctacaaca
      301 acaagaattc ccaaatcggt aaattattat cgttaaaact ccttgagagg gaaagcactt
      361 taaagatatg atactgaacg tatatggcga tgatgaagct ctcaacagca tcttggagga
      421 ccttgactac atagttggtg ttaagattct ttctaggcgc gacttagagg acgggagggg
      481 agtgttgatc atccaagcga tgcggtatac aaaatatgca ataataaaat tagcaaagaa
      541 gtatgagtta ggggtaagtt gaagttatcg gacttgtacc cagccctgcg aagtacctca
      601 ttcccgctcc gctcccctcc gagcctccgc ttcgctacga ctccgacccg cccccgcagg
      661 ccagcgcctt acattgcgat gcaaggaata acaccagaac aaggcacagt ggcgggtctg
      721 cgtctccgct ccgctgcgcc tctccgttcc gctccacttc cttccatcgc cctcactatt
      781 gttttttgct ctgcggagca aaaaagggaa cgaaaattaa ggattgacct atcctggatc
      841 actgtttata gataggggat tgttacctga ttgttctgtt ttcttgtcga aaaaaccaaa
      901 aatcactgga ttttggaatt gtgcccgttt gcagcaaata tttataaaat attcttaaat
      961 tttatatcac aaaattgata ttttttttaa cacccctctc aaatccacta ttgggaatga
     1021 tattatgatt tgctccctct tcataaagct ccacatcatc aaaccagaac gtcccatagc
     1081 cgtcccatat attcgcatat acgtaaaccc atcttgtatt attgttcgtt tttgttttgg
     1141 tctgcttttt cgtccagtta tgtgttcctt tactgaaaac tagattggtc tgtctcatcc
     1201 acttaccatt agtatccaat tctacgactc gaaccgcagg tgaattattg cctcccgcac
     1261 cctctatttt gctccatgat gaaaaagtat aggtggtatt tggtttcgca ggaattttat
     1321 tgcttctcca taaacctgat tttttacttt tattccccga tatctcgatc ttggctgaat
     1381 ggctgccatt atgagaaaca gttgaatccc acgatgcatt ctgggtaaac tgccatctat
     1441 ccacaatgtt gcctcctccg acttcatctt tcaaatatcc atcataatac tcaaacatag
     1501 gtattgtcat aactaaaaat attcctccca cagcaaatgc tttcatagtt ctgcctaaac
     1561 cactatttcc ttcatacaaa gtcctgaccg ttccgcacaa aatcatcgca attatccccg
     1621 caagaagaac ttcggtattg gcgataccat accaatctgg ctgataataa ataatcccaa
     1681 taccaaaaac atgctttgac aatggccaca agaacgcata cgccggattg taaaccagtg
     1741 catcctcgaa gaagtgagca ctaaatccta ttccagcgaa tatgaatgaa tctttgaact
     1801 tcaaaccggt catcttcaat aacaatccaa caaatcctgc aaaaaggagt agaactaaaa
     1861 tattatgaaa atcaccgtgt tttaaaggat tcccattgat aagaattcca atatcgaatt
     1921 tattaaatat atatcctgta aacatatcaa gatctggagc caacgcactt gctatgacga
     1981 tccaggagta gtcgcggcct gtttttttaa aatggatcat gcccgctatg atcgcgatgg
     2041 ccgtagagta tatccagtgt tctaagagca tgatttctag tattagtatt gggaataaat
     2101 attaagacgc cctaattgaa aataccttcg ccacctcgtt agtgggaata agatgaattt
     2161 tgagatgcat catacataat tatcgtatac cacgcttacc atcctaagac ccacgagacc
     2221 ataccttatt gtttttttgc tctgcggagc aaaaaaaggg aacgaaaatt aaggataggg
     2281 tgtggtatgg gggttagcgc atacgtggga aggcttttgc gtcataacgg cgctaacaat
     2341 atcatgtttt gcaaatacgg ttacatttcc gaattattaa agtatctcta ttttatgtat
     2401 aaatgatgat ttgtcttttt tggcagtcct ttctaatatt ttatcatgct ttttcactat
     2461 ttctccatgc tctggatctt cttcttttaa cacagtatct gcttcatcaa ataattcttt
     2521 aattttaatg ttatctacat tatttttgat ttcatcagga aattttagta tatatataat
     2581 ttctgcagat cctgcttctt ccagagatat tttttgcaga tcaatagctt ctatttctat
     2641 atttagatgt ttacatatat ttataagtcg tacaaaagaa ccaggcaaat tcaatgtctc
     2701 aatgttgatt ctaaatatat tggtctcata aaactttaga ataagcatac caacatctaa
     2761 ttcaactatt tggatgcatt tgcaagcgtc tgcgcctttt aggttagcac gttctttaaa
     2821 atcatcacta attttataat atattgtcca attttttgaa atttttactg atttcaagct
     2881 ctttttaaaa atatcttgat attgtattaa aggtacaaca atatgtttta ctccctcatc
     2941 ctttttttca tcctcagaat actcaattgt atctaatacc tttattttat cttccgtgtc
     3001 attttctgga atttttttta agattgtttc aagactattt ttttcccatt ttgaccgaat
     3061 ataatcggga acttcatata gaagagtcgc ccgatattta ctatctcttt tataaaagtt
     3121 aatagaaagg atatttattc ttcgctgcga tagtgctact ccaagttttt tgaaatattc
     3181 aatatcacta aaacatataa ttgaatgata cattgaacgt ttaagttcat atacgcaaag
     3241 aacaagttgc atgttgtcta aatcttcata aatataaata tcttgatccg gttttaggtt
     3301 tagtgacgtc ctaccatatt gtcccagacg gatcactgga tttttgaagt tgtaatcgac
     3361 aacctcgaga ggcgtaggta gcacttttgc ttttgaggat ttcaacatga ttcatagaaa
     3421 gatgtaataa catataaaat tttatattca agctcttcat tccaacgaaa accactgatt
     3481 taatgttgtc ataccccata ttagtcaatc aaatacatag tatattgaat actactaaat
     3541 gatattaaat actatataaa tttagtaaca ataacattaa gaaatatatt tatccacaat
     3601 agttttgatc attcaagaaa aagtgttcaa accaaaagtt ttatttacca ttaatgtcat
     3661 gattaataat ggcaggaata aacaagcttg cattgtgccc tcataatttg tctttgcact
     3721 ggtggtataa agcgagcgca attatccgtg tccatggaac atctttcaat aggaggtata
     3781 aatgagctat aaaacacaaa gaggaaatat cctcaaaaga gctataggaa tattggtgct
     3841 tgtcagtatt aggggtagta ccagcgctgc tgcaaccatg ccc
//
LOCUS       DATGBN010000118         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_47739, whole genome shotgun sequence.
ACCESSION   DATGBN010000118 DATGBN010000000
VERSION     DATGBN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47739"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..719)
                     /locus_tag="VKL21_02575"
     CDS             complement(15..719)
                     /locus_tag="VKL21_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44685.1"
                     /translation="MVDWNDNTVKIVVYLIYGTSFLIMFAALTFWKKRVSHIEIMDDF
                     KYLAAFGLLHGLAEYSDIPRFLAWQPSWIFDLVKLLLVSSSFAALLAFGLNVISAGLE
                     ERRWLRGIPFGAFLMYTWLLVFAGLDFSNLDAGINYKVADLAQRYSMGLLGAAFTSYA
                     FFELSGKMNEIAGKSAGKKFMYAGIGFALYAIFGGMIVTPVLGIPVVVYRSAIAVLIT
                     IAVIGIFRMFDVKGSK"
     gene            complement(1098..1313)
                     /locus_tag="VKL21_02580"
     CDS             complement(1098..1313)
                     /locus_tag="VKL21_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44686.1"
                     /translation="MFLKRPRICVRKFVKCRKSTNAANSSRQLLNYFPGDKKLKIING
                     NSHGTDMFLPESGIMDKLVAWAIIKMK"
     gene            complement(1575..>1774)
                     /locus_tag="VKL21_02585"
     CDS             complement(1575..>1774)
                     /locus_tag="VKL21_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44687.1"
                     /translation="TETQVTNVLLAFLLTNGITRRIIVENPEVFMSPEYCFDELWEHR
                     DRWNKNHLPDSELIEVVNDVR"
BASE COUNT          551 a          399 c          304 g          520 t
ORIGIN      
        1 ttgcttatgc agccttattt cgatcccttc acatcaaaca tcctgaagat ccctataact
       61 gctattgtta tcaaaacagc tatcgcagaa cgataaacaa ccacaggtat gccaagaaca
      121 ggggttacga tcatacctcc gaatatggca tataaggcaa atccaatgcc tgcgtacatg
      181 aattttttcc cggcgctctt accggcaatt tcattcattt ttcctgataa ttcaaagaat
      241 gcatatgatg taaatgccgc acccaataat cccatactat atcgctgtgc caggtccgct
      301 actttataat taatacctgc atctagattt gaaaaatcaa gtccggcaaa aaccaacaac
      361 catgtataca ttaagaacgc cccgaacggt attcctcgaa gccatcttcg ctcttctaat
      421 cctgcggaga tcacattcaa cccgaaggca agaagagccg caaaggaact tgaaaccagt
      481 aataacttga caaggtcaaa aatccaggag ggctgccatg ccaggaatct tggtatatcc
      541 gaatattcgg caagaccatg aagaagaccg aacgctgcca ggtatttgaa atcatccatg
      601 atttctatat ggcttaccct ttttttccag aaagtcaatg cggcaaacat tataaggaag
      661 cttgttccat atattaaata caccacaatt tttactgtgt tgtcattcca atcgaccata
      721 attaattctc cttgaatata tctgataata ttatacgaat caatatttaa atctaattac
      781 tatttatgat tgtaataatg agatatacct atggcgtcaa taaactgtta agatatattc
      841 aaacctctgg ggtctgaata attggaaccg cagataagag tactattaac gctttcggtc
      901 aacattatct taacggcgct ggggtgcgac cccagacccc ggcggatgcg ccgctgctgg
      961 cggggtttaa catgaaatca gcggttgcat attattatac catccatact tgcatgtatt
     1021 aataagaaaa atagatagca tccgcgcgca tccgcataaa ttagaaaaaa aggaattcta
     1081 tcgaatttgc cacaggctca tttcattttt atgatcgccc aggctactaa tttatccata
     1141 attcctgatt ccgggaggaa catgtcagtt ccatgagaat ttccattgat tatcttcaat
     1201 ttcttatcac ccggaaaata attcaataat tgcctgctgc tgttggcagc attagttgat
     1261 ttccggcact tcacaaattt gcggacgcaa atccggggtc tctttaaaaa cataattgtg
     1321 catagttgcg gatttcaagc cttatgatca ttcctttaaa ggatatagcc cgattacatc
     1381 ttttgtggac aataccctta tgcgttctcc aaaaacctcc tgtctaaagt gctttgtatt
     1441 gtacgtccag attccttcat tatccacaga taaacaaagc gcaataatag gtgcgtccct
     1501 ttatcatccg taagtttttc accttctgaa atatacggat tgtaaacatc aggagataca
     1561 gccatcacaa ataatcacct tacatcgtta acaacttcaa tcaattcact atccggaagg
     1621 tggttcttat tccatcggtc cctatgctcc cagagttcat caaaacaata ttcaggagac
     1681 ataaatactt cagggttctc aactataatt ctgcgtgtta tgccgttagt gagaagaaat
     1741 gctagcagga catttgtaac ctgagtttct gtca
//
LOCUS       DATGBN010000119         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_242094, whole genome shotgun sequence.
ACCESSION   DATGBN010000119 DATGBN010000000
VERSION     DATGBN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242094"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VKL21_02590"
     CDS             <1..566
                     /locus_tag="VKL21_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HMB44688.1"
                     /translation="RHGIPVRVKNTFHPDVPGTIVVKDQKQSKDIVKAVTVIKNVALI
                     NISGAGMVGTIGVAARVFTTLANAGVNIVMISQGSSEANISLVVDESHLDRAVKAIKS
                     EFKNGIIKEVTPDRNISVVAVVGAGMANTPGVAGRVFGALGKARVNVIMISQGSSQHN
                     ISFAVSEEDAKKAVSVLHKEFGLDRVK"
     gene            581..754
                     /locus_tag="VKL21_02595"
     CDS             581..754
                     /locus_tag="VKL21_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44689.1"
                     /translation="MNSLPVLSKIILVIGFTLGIMSLIIFLRYPIMLILMKYSPKYRE
                     FIKKNLARKKSRK"
     gene            759..1322
                     /locus_tag="VKL21_02600"
     CDS             759..1322
                     /locus_tag="VKL21_02600"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HMB44690.1"
                     /translation="MRKIIFVTGNPHKVREATDIMSPLGIKIVQNNCGYPELQEDTLE
                     AISGFGARWTANKLNGEVMVDDSGIFIDALSGFPGPYSAYVARTIGNGKILKLLEGEI
                     NRRVSLKCVIGYCRPGEEPVVFSGEVIGKIAEDIRGELGFGYDPIFEVGGSTFGEMAD
                     EEKNRISHRYMALSEFARWLKETDSAF"
     gene            complement(1382..1555)
                     /locus_tag="VKL21_02605"
     CDS             complement(1382..1555)
                     /locus_tag="VKL21_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44691.1"
                     /translation="MSSEDDKMKLHYMQLITTETDGLTVKTIHSEVRGESMEQCFEYT
                     KKLHNGEVKSKNH"
     gene            1793..1960
                     /locus_tag="VKL21_02610"
     CDS             1793..1960
                     /locus_tag="VKL21_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44692.1"
                     /translation="MKRHEKIDATTDTVVESNSVMPVSDGSRKLSAVASVHSIALNIF
                     GIISVHVQEAN"
     gene            complement(2170..2391)
                     /locus_tag="VKL21_02615"
     CDS             complement(2170..2391)
                     /locus_tag="VKL21_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44693.1"
                     /translation="MEEEESKYITKTVNDLTVKIDKKIDELSKSIEKTLGVKQEYAEG
                     KINENPLAYMAGAFVGGVIVGYIMSRGKS"
     gene            complement(2560..2700)
                     /locus_tag="VKL21_02620"
     CDS             complement(2560..2700)
                     /locus_tag="VKL21_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44694.1"
                     /translation="MAEHVTDTSERKLEDAAAAKLKKEAEAKSKAAGSSEKSETHKKA
                     EK"
     gene            complement(2717..2854)
                     /locus_tag="VKL21_02625"
     CDS             complement(2717..2854)
                     /locus_tag="VKL21_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44695.1"
                     /translation="MAEQGSMQAEAKLKEAQAKLKEAEAKLVESEGKLEECEMHLKGE
                     K"
     gene            complement(2869..3006)
                     /locus_tag="VKL21_02630"
     CDS             complement(2869..3006)
                     /locus_tag="VKL21_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44696.1"
                     /translation="MEEQGSIQAEAKLKEAQAKLKEAEAKLVESEAKLTECESHLKTE
                     K"
BASE COUNT         1126 a          735 c          788 g         1367 t
ORIGIN      
        1 tcaggcacgg gatacctgtg cgcgttaaga atactttcca tccggatgtt ccaggcacaa
       61 ttgttgtaaa agaccagaaa cagagtaagg atatagtaaa agccgtaacg gttatcaaga
      121 atgtggcgct gatcaatatt tcgggcgcag gaatggtagg aacgatcggt gtcgcagcgc
      181 gcgttttcac tacacttgca aatgcgggcg taaatatcgt gatgataagc cagggctcct
      241 cagaagcgaa catttcactg gtagtcgatg aatcccatct tgatagggct gtaaaggcca
      301 taaagtctga attcaagaat gggatcatta aagaggtcac gcctgacagg aacatctccg
      361 tagtagccgt tgtgggcgca ggaatggcaa atacacctgg cgttgccggg agagttttcg
      421 gggcgctagg taaagcccgg gtaaatgtga taatgataag ccagggttca tcccagcata
      481 atatctcatt tgctgtaagt gaagaagatg caaagaaggc tgtttccgtg ctgcataaag
      541 agtttgggct tgaccgggta aaataatagt catgatggat ttgaacagtc ttcctgtatt
      601 atcaaaaata atccttgtta ttggttttac tttaggcatc atgtcattaa ttatttttct
      661 gcgttatccg ataatgctga tattgatgaa atacagcccg aagtaccggg aattcataaa
      721 aaagaacctt gcaagaaaga aatcccggaa atagctcaat gagaaaaata atctttgtta
      781 caggtaatcc ccataaagta agagaagcaa ctgatattat gtctcccctg ggcataaaaa
      841 tagtgcaaaa caattgtgga tatcctgaat tacaggaaga tactcttgaa gctatctcgg
      901 gttttggggc aagatggact gcaaataaat taaatggcga ggtaatggtg gatgattccg
      961 gtatattcat cgatgctctg tcgggattcc ctggacctta ttccgcgtat gttgccagga
     1021 caatcggaaa tgggaaaata ttaaaacttc ttgaggggga aattaacagg cgcgttagcc
     1081 tgaaatgcgt gatcgggtac tgccgccctg gtgaggaacc tgtggttttt tcaggggaag
     1141 tgataggaaa gatcgcagaa gatatcaggg gagaactggg tttcgggtat gatcccatat
     1201 tcgaggtggg tggatcgaca ttcggtgaaa tggctgatga ggaaaagaac aggatctccc
     1261 acaggtacat ggcgctgtct gagtttgcgc gatggttgaa agagactgat agtgcctttt
     1321 gatgcgatgt tcgctaaagc cctgaatatc taatcatcga gggattgaaa taagtaatcg
     1381 ttcagtgatt ctttgatttt acttcgccat tatgcaattt tttagtatat tcgaaacatt
     1441 gctccataga ttctccccgt acttcagaat gaatggtttt cacagtcaat ccatcggttt
     1501 ctgttgtgat taactgcata taatggagtt tcattttatc gtcttcgctt gacatttggt
     1561 tctcctttta cttcagtttt tgtttacctt tcatgacttc tttgagttga ttatcattat
     1621 aatcatacac tacttgaagg gattaaatat atatatcctt tctggtcagt tttcgactat
     1681 cttttcccaa aaaagatatc ctcattctaa ataaatgtac ctatatagta aaaatcaaga
     1741 acaagcgaaa gatatgccca tttgagcaaa agcacagtta aagaaacgca agatgaagag
     1801 gcatgaaaaa attgatgcga ccactgatac tgttgttgaa tctaatagtg ttatgccggt
     1861 atcagatggc tcaagaaaac ttagcgccgt agcttctgtt cattcgattg ccctgaatat
     1921 ttttggtatt atttcggttc atgttcagga ggcgaattag gtacagggat aggttgaggt
     1981 tcaggaatcg gttcggggat aggtttagga ttaggcggtg gttgttgata cttgttcgat
     2041 tcaatgcttt ctttgttgct gaagtttctc atatagtcac tctccacatt tttatataat
     2101 tcaattgaaa taaaatataa ataattgatt ttgaaaggtc gctaagaaaa tctattatac
     2161 tttgctgtgt tagctttttc ccctgctcat aatgtaacct actatcaccc cgccgacgaa
     2221 tgctcctgcc atataagcaa gaggattttc gtttatcttc ccttcagcat attcttgttt
     2281 caccccaagt gtcttctcaa ttgatttaga aagttcatca atttttttat caattttgac
     2341 ggtcaggtca ttgactgttt tagttatata ctttgattct tcttcttcca taaaagtaac
     2401 ctccacaatg taatttatgt aatgtctata tatatttttc agtccagctt tgtgccggta
     2461 gtcagcgtta ttgatttcag cgagtttatt ttttcgcttc caaaagaaag aaaccaatag
     2521 actttataca tccattagct tctccttttc tgaagttatc tatttctctg ctttcttatg
     2581 cgtttcactc ttttcagaag atcctgctgc ttttgattta gcttcagctt ctttttttaa
     2641 tttagctgct gctgcatctt ccaatttacg ttcgcttgtg tccgttacat gttctgccat
     2701 attttccaac ctccttttat ttctctcctt tcaggtgcat ttcgcactct tctaatttac
     2761 cttctgactc tactaatttt gcctctgctt cttttaattt agcttgcgct tcttttaatt
     2821 tagcttcagc ttgcatgctt ccttgttctg ccatattttt cactcctttt atttctctgt
     2881 tttcaggtga ctttcacact ctgttaattt agcttctgac tctactaatt ttgcttctgc
     2941 ttcttttaat ttggcttgcg cttcttttaa cttagcttca gcttgtatgc ttccttgttc
     3001 ttccatattt ttcactcctc attatagtct tccgcaaact tctgagaatt tcaaacagtc
     3061 gtactaatgc gaactgatac gaactcgtga gttcgttgca tttagttgtt tttatcatac
     3121 gttcggcgga gaactatata ttaaataatt aattaagcaa ctcccaggct taattaataa
     3181 tcaaaatcag ttctcatatt aattaaacct ttcggttacc acggctgtgt gtgtagtccc
     3241 gaaatctttg gcttgatata cttatcgtct aaatgcagga tgaaaaataa agtaatgtct
     3301 aattgagata actgcgagcc aaaacaagcg aaaaggttat aaacattctg ttgaaatagc
     3361 atctatgagg aggttagata atggatttaa tagaaacatt aattgtgatt ctggtgattc
     3421 tatggctatt ggggtcgctc ggttcagttg gtgctttaat caatatattg ttggtcattg
     3481 ctatcatctt gatactgctc agagtcatcg aaggtcgtag gccgttataa taggttcgtc
     3541 ctgagggttc atagatataa catttcaaat atctaaactt ctattttttt cctccgatat
     3601 gctgtaaaaa gatgacttca ccatatttgc tatcaatcat tttttccatt gtcgtttaaa
     3661 attaaatgaa cgattagaat attttaaata ctatgcaggt attcattgta ttgtttaccg
     3721 gggagtgaac aaaggagtgg gaatttacag tgcctgcatc agtcgcctat cagacaattc
     3781 gaatcatgag gcaggcacaa tccatatatg caaagaaggt taaaaaaaca tcaaaaggaa
     3841 aaaagaaaat acaactcttt aaattatcct taatgaaagt taagatatga ttttttctcc
     3901 ggaaattttg tatgcccatc tactgaaact atttatataa tatatttctt cactattatt
     3961 cttttcgata aattgaattt ttccatcctg tacttcacca gattcaagtc tgaatc
//
LOCUS       DATGBN010000120         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_125864, whole genome shotgun sequence.
ACCESSION   DATGBN010000120 DATGBN010000000
VERSION     DATGBN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125864"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VKL21_02635"
     CDS             complement(<1..533)
                     /locus_tag="VKL21_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44697.1"
                     /translation="MNDYALGIKELDNAIGGIRKGSNIMLIGPPMSGKELILNFIMYH
                     GAKINENAVITVTTRESATHILEWFKENKLNLPLDRIGIIDCVSKTLGGAIKEDENIR
                     IASSPVDLTGIGVKISYFFEIFITKKNIQKNQLHMNSLSTILMYSNIQTVFRFLHVFT
                     GRIKSIGGLGIYLIESG"
     gene            complement(530..793)
                     /locus_tag="VKL21_02640"
                     /pseudo
     CDS             complement(530..793)
                     /locus_tag="VKL21_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            complement(812..1342)
                     /locus_tag="VKL21_02645"
     CDS             complement(812..1342)
                     /locus_tag="VKL21_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB44698.1"
                     /translation="MIAGKQILVVEDEVITAMDIQRRLKNQGYIVPVTVSSGEEAIKK
                     VKENNPDLVLMDINLKGEMDGIEAASKIHSFSDIPVIFLTAYADDKTLVRAKITEPYA
                     YMVKPFKDRELQINLEIAFYKNAMEKKLKESYETLREKNQWLAAVIESIGDAVIATDP
                     EGTIRLMNPIAEALTG"
     gene            complement(1339..>2359)
                     /locus_tag="VKL21_02650"
     CDS             complement(1339..>2359)
                     /locus_tag="VKL21_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMB44699.1"
                     /translation="TECKQAEEELLKFKLGIERSDEVIFITNIDGSIIYINPAFEKIY
                     GYSREEALGKTPRIIKSGLLPSEAYKTFWDTLLEKKVVNGELINKTKDGRLLNIDGSA
                     NPILNDDGNIVGFLAIQRDITERKLAEEHIKKSLEEKEVLLREIHHRIKNNMQIVSSL
                     LMLQSQNIEDKKYKDVFIDSQNRILSMALIHEKLYQSEDLAQIDFKEYINEMVSNIFS
                     SYGLNSNIKIDINVENIPINIDNAVPCGLIINELVTNSLKYAFPEGRRGKIQISVKSK
                     EKNMIQLSISDDGIGIPKDLNIRNTKTLGLTLITALAENQLNGELIINREKGTEFQIS
                     FRGTK"
BASE COUNT          625 a          472 c          385 g          877 t
ORIGIN      
        1 atgccgcttt ctatcagata tattccaaga cctccgattg attttatccg tcctgtaaaa
       61 acatggagga acctgaaaac tgtctggata tttgaataca ttaaaattgt tgaaagtgaa
      121 ttcatgtgga gttggttttt ctggatattc tttttcgtta tgaatatttc gaagaaatag
      181 ctgattttca ccccaattcc tgtcagatca accggactgc ttgctattct aatattttca
      241 tcctctttga tcgcaccacc aagggttttg cttacgcagt cgattattcc gatacggtct
      301 aaaggcaaat tcaatttatt ttctttgaac cattccaaaa tatgagtggc tgattcgcgg
      361 gttgttacag ttattactgc attctcattt atctttgcac catgatacat aataaaattt
      421 aggattaact cctttccact catgggaggg ccgatcagca taatattcga gccttttctt
      481 atgcctccga tcgcattatc cagttctttt atgccaagtg cataatcatt cattgtttca
      541 gggccccatt gatccttgtg cgaccaataa tatcatagaa tatcaataca ataccaatta
      601 tattatttcc atcaatttta atggttgaac caataatatc aacaggtatt tttatacctt
      661 cttttgttac caatacagta tggtccgcaa gaccgtagaa catgtcctct cgaattgcct
      721 ttgttacagg gttttcaatt tttatgtcag tttcttcgct aataatattg aaaacgtttg
      781 tcaaaggctt ccctaaagct tcattctgct ttcagccagt taatgcctct gcgattggat
      841 tcatgagtct tattgttcct tccgggtctg tggcgatcac tgcatcacct atgctttcaa
      901 ttactgctgc aagccactgg tttttctccc ttaacgtctc ataactctct tttaacttct
      961 tctccattgc gtttttatag aaagcaattt caagatttat ttgtaattcc ctgtctttaa
     1021 atggttttac catgtatgca taaggctctg taatttttgc ccgtacaaga gttttatcat
     1081 cagcatatgc ggtcaggaat attactggaa tatcagaaaa agaatgaatt tttgatgctg
     1141 cctcgatccc atccatttca cccttcaggt tgatatccat taataccaga tcgggattat
     1201 tttcttttac ctttttaatg gcttcttctc ctgaggatac tgttacaggg acaatatatc
     1261 cctggttttt caaccgtctt tgtatatcca ttgccgtaat tacttcatct tcaacaacca
     1321 gtatctgttt tccggctatc attttgtgcc tctaaaactg atttggaatt ctgttccttt
     1381 ttctctattt ataataagct caccattaag ctgattttcg gcaagagcgg tgatcagagt
     1441 caaacccaat gttttggtat ttcgaatatt cagatctttc ggaataccta ttccatcatc
     1501 acttattgaa agttgaatca tatttttctc ttttgattta actgaaattt gaattttccc
     1561 ccgtcttcct tccggaaatg catattttaa ggaattagtg acaagttcat taattattaa
     1621 tccgcaggga actgcattat caatgtttat cggaatgttt tctacattaa tatcaatttt
     1681 tatattgcta tttaggccat aagatgaaaa tatatttgag accatttcgt taatatactc
     1741 tttgaaatca atttgtgcaa ggtcctcgga ttgataaagt ttttcgtgga taagggccat
     1801 tgaaagtatc cggttttggc tatcaataaa aacatcttta tactttttat cttcgatatt
     1861 ttgggattga agcataagca aacttgaaac tatctgcata ttgtttttta tacgatgatg
     1921 gatttcccga agaagaactt ctttttcttc gagagatttc ttgatatgtt cttcagccaa
     1981 ttttcgctca gtgatatcac gctggatcgc caggaaaccg acaatgttcc cgtcatcgtt
     2041 caggatggga ttggctgagc cgtcaatatt caagaggcgg ccatctttgg tcttattgat
     2101 caattctccg tttacaactt tcttttcaag aagcgtatcc cagaatgttt tatatgcctc
     2161 agaaggaagt aatcctgatt ttataatacg gggtgttttt cccagtgctt cttccctgct
     2221 gtatccgtat attttttcga aggcaggatt tatataaatt atcgatccat caatattggt
     2281 tatgaaaata acttcatcag atcgctcaat cccaagtttg aactttaata actcctcttc
     2341 agcctgtttg cactcggtg
//
LOCUS       DATGBN010000121         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_242115, whole genome shotgun sequence.
ACCESSION   DATGBN010000121 DATGBN010000000
VERSION     DATGBN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242115"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VKL21_02655"
     CDS             <1..277
                     /locus_tag="VKL21_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44700.1"
                     /translation="EDAVSKDISKILEDDFFKRIGFTVRECNLLGSQRKGYFLYIKAN
                     PEDIELAEKKFEGAGLTKLIGEEIKIVTDAFLAEEENAASGMGMIFG"
     gene            399..905
                     /locus_tag="VKL21_02660"
     CDS             399..905
                     /locus_tag="VKL21_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMB44701.1"
                     /translation="MSKIVVVYLSTSGNTKAMADAIAEGIISRNVSVTTMNFHEARIE
                     EIKAADAIAIGSSTFHYKMLPPMEKFMDSLEKANVKGKVGAAFGSYGWSGEAPINIAD
                     KMRKLGMEVIDPVLRIQYQPTDKDLEECKRLGKDLASKVKKTAEKSGVKKAEGTPLQD
                     VKMGEGGH"
     gene            complement(1105..1629)
                     /locus_tag="VKL21_02665"
     CDS             complement(1105..1629)
                     /locus_tag="VKL21_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HMB44702.1"
                     /translation="MKQKLTVDIEKDLKLPGNVWRFGDDIDTDAIIPGKYLTINKPDE
                     LARYAFEGVCPKFAQGVKEGDIIVAGFNFGCGSSREHAPLALKGARIKCIIAKSFARI
                     FFRNAINIGLPLLECPDTDKIEESDNIEVDFGSGLITNTTKKETYQATPLPDFVRGIV
                     DAGGLIRYAQKMVE"
     gene            complement(1690..1992)
                     /locus_tag="VKL21_02670"
     CDS             complement(1690..1992)
                     /locus_tag="VKL21_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HMB44703.1"
                     /translation="MEFCPKCKSMMMPAKNVFTCRKCGFEKDIKESFVSTTERKEREV
                     TVLEGREMGMPTIRAKCEDCGNDTAYWWLRQLRSADESEVRFFRCTKCGKTWREYN"
     gene            complement(1970..2407)
                     /locus_tag="VKL21_02675"
     CDS             complement(1970..2407)
                     /locus_tag="VKL21_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMB44704.1"
                     /translation="MNTKETRFIPITPLLTVDALILYDNKIVLIKRMNPPYQNQFALP
                     GGFVEVGETVEAATLREAKEETGLKIDIIKLLGVYSEPSRDPRGHTVTVCYLARGFGK
                     LKAGSDAKDIRLFGFNEIPKLAFDHNKIIENAKCDIDGILSQM"
     gene            complement(2404..3495)
                     /locus_tag="VKL21_02680"
     CDS             complement(2404..3495)
                     /locus_tag="VKL21_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMB44705.1"
                     /translation="MAIEEDIKAIEEEISKTKYNKATEGHIGRLKSKVARLRDEVQKR
                     ASSKTGGEGFSVKKSGDASVVLVGFPSVGKSTLLNSLTGTESAVAAYEFTTLDVVPGA
                     MEHKGATIQILDLPGLVRGAASGRGRGKEVIAVIRNADLTVILLDVFQLVHYDVLLQE
                     LYEAGIRVNSKPPDITIKKKARGGVNISSTVDLDLDEDTIKTILGEYRIHNANVLIRE
                     NISIDQLIDAVLSNRKYLPGIVVINKIDLADEYALRACKEKFPDGILVSADKKVHIEE
                     LKDRLFEKLGFIRIFMKPQGKAADLEEAMIIRYGATIGDVCDKLHRDFRKRFRYAQIW
                     GTSAKHEGQRAGLDHNMDDNDILTLVLRR"
BASE COUNT         1004 a          787 c          704 g         1039 t
ORIGIN      
        1 tgaagatgct gtcagtaaag atatttcaaa aatattagag gatgattttt ttaaaaggat
       61 cggttttacg gtaagggaat gcaaccttct gggttcgcag cgtaaaggtt atttccttta
      121 tataaaagca aatccggaag atatcgagct ggcggaaaag aaatttgaag gagctggact
      181 tacaaaactc attggagagg aaattaaaat agtcacagac gctttcttag cagaagaaga
      241 aaatgcagcc agcggaatgg gaatgatttt cggttaaaat caaacaatag ctattaataa
      301 tgctaaggtt cttaataata tatggtcagt ttgctcaaaa aacgcaataa aaataaaaga
      361 acagcgaacg gctttagccg tgaggagagg ttagtaacat gtcaaaaata gtagttgtat
      421 atctcagtac atcaggcaat acaaaagcga tggcagatgc aatcgcagaa ggtattatat
      481 caagaaatgt aagtgtaacg accatgaatt ttcacgaggc aagaatagaa gaaattaagg
      541 ctgcagatgc aattgcaata ggttcttcaa cattccacta taaaatgctt ccacccatgg
      601 aaaaattcat ggatagtctt gagaaggcaa atgttaaagg taaagtaggg gcagcattcg
      661 gttcctatgg gtggagcgga gaggcgccaa taaatattgc ggacaaaatg cgcaaacttg
      721 gcatggaggt aattgaccct gtcttacgta ttcaatatca acctacagac aaagatcttg
      781 aagaatgtaa gaggcttgga aaagatttgg caagtaaggt aaaaaagacc gctgaaaaat
      841 ctggagtcaa aaaggcagaa ggtacgcctc tacaggacgt taaaatgggt gaaggagggc
      901 attaagataa gaaacaatca caaaattttg tttcgataag ggctataaaa ctttacaaaa
      961 gctcaaatcc cataaaataa ataaatttca gacaggattt acaggatcga caggatttga
     1021 ttgaaaagtt atcctgtaaa tcctgttatc ttgtcaaaag gtgcctttgc ctggctcagt
     1081 aattcccttt atcatccccc acagttactc taccattttc tgcgcatacc tgataagacc
     1141 gcctgcatca actataccgc gcacgaaatc cggaagcggc gttgcctggt aggtctcttt
     1201 tttagttgta ttggtgatta acccggatcc aaaatcaacc tctatattgt cgctttcttc
     1261 gatcttatca gtatccgggc attcaagaag tggaagtccg atattgatgg cattccgaaa
     1321 gaagatacgt gcgaatgatt ttgctatgat gcatttgatc ctggcgcctt ttaatgcaag
     1381 aggcgcatgc tctcgcgatg aaccgcatcc gaaattaaaa cctgcaacaa taatatcccc
     1441 ctcttttacg ccctgcgcaa atttcgggca tactccctca aaagcgtatc tggctaactc
     1501 atctggttta ttaattgtga gatatttgcc cgggatgatg gcatctgtgt caatgtcatc
     1561 cccgaatctc cagacatttc ccggtagttt caggtctttt tcaatatcaa ctgtgagttt
     1621 ttgtttcatg ggaggttgaa ttgcccggat tggtaataag atttattcag aattatttca
     1681 tattgatttc taattatatt ctctccaggt cttcccgcat ttcgtacatc ggaagaacct
     1741 cacctcgctc tcatcagccg agcgaagctg ccgcagccac caatatgccg tatcatttcc
     1801 gcaatcctca cacttggccc ggattgtggg cattcccatt tcccttcctt caagaaccgt
     1861 aacttccctt tcctttcttt cggtcgttga aacaaaactt tccttaatat ccttttcaaa
     1921 cccgcatttc ctgcacgtaa aaacattctt ggcgggcatc atcatacttt tacatttggg
     1981 acaaaattcc atcaatatca cacttcgcgt tttctattat cttattatga tcaaatgcca
     2041 gtttgggtat ttcattaaac ccaaagagcc ttatgtcttt agcatccgaa ccagctttaa
     2101 gctttccgaa acctcttgca agataacaca cagttactgt atgaccccgt gggtcgcgtg
     2161 atggttccga ataaacacct agcagcttga tgatatcaat tttaagcccc gtttcttcct
     2221 ttgcttcacg aagagtggct gcttccactg tctccccgac ttccacaaaa ccgccgggaa
     2281 gcgcgaattg attctgataa gggggattca tgcgctttat caagacaatt ttgttatcat
     2341 acaatatcaa tgcatcaaca gtcaaaagcg gtgttatggg aataaatctg gtttctttag
     2401 tgttcatctt cgtaaaacaa gcgtcaggat atcattatca tccatgttat ggtcaagccc
     2461 cgctcgctgt ccttcatgct ttgccgatgt accccatatc tgagcatacc tgaaccgttt
     2521 cctgaaatcg cggtgaagct tatcgcatac atctccgatc gtggcgccgt atcttattat
     2581 cattgcctct tccagatcag cggccttgcc ctgcggcttc ataaaaatcc ttataaatcc
     2641 gagtttttca aaaagcctgt ccttgagttc ctcaatatga actttcttgt cggctgaaac
     2701 caggatccca tccgggaatt tttctttaca ggcgcgcagg gcatattcat ccgcaagatc
     2761 gattttattt atgaccacta tccccggaag gtatttcctg ttgctaagga ctgcatctat
     2821 aagctggtcg atcgagatat tctcgcgtat caaaacattc gcattatgga tcctgtattc
     2881 tccgagtatt gtttttatgg tatcttcatc aaggtcaagg tcaaccgttg aactgatatt
     2941 aacgccaccc cttgcttttt tctttattgt tatgtccggg ggttttgaat tgacgcggat
     3001 acctgcttca tataattcct gtaacaggac atcataatgc acaagctgga aaacatcaag
     3061 caatataacc gtaaggtcag cgttccttat aacagcaatg acttccttac cgcgcccgcg
     3121 gccggatgcg gctcctctta ccagtcccgg aagatcaagt atctggatgg tcgccccttt
     3181 atgctccatt gcccccggca ccacgtccag ggtagtaaat tcataagcgg caactgctga
     3241 ctctgtcccc gtaagactgt tcaggagcgt gctttttcct actgacggga atcccacaag
     3301 cacaaccgat gcatctcctg atttctttac tgaaaacccc tcgcctccgg tttttgaaga
     3361 agctcttttt tgtacttcat cccgcaggcg ggcaaccttt gatttaagac ggcctatatg
     3421 accttcggta gctttattat attttgtttt ggatatttct tcttcgattg cctttatatc
     3481 ttcttcaatt gccatcgaac cttatagcta taaacaaggc aaaaagcatt tggg
//
LOCUS       DATGBN010000122         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_475246, whole genome shotgun sequence.
ACCESSION   DATGBN010000122 DATGBN010000000
VERSION     DATGBN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475246"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VKL21_02685"
     CDS             <1..367
                     /locus_tag="VKL21_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMB44706.1"
                     /translation="YACFNDVTARDLQKEDVQWTRSKSFDTFAPVGPFITDPGLDVKD
                     LSIKTRVNGKLRQDSRTSNLIFSLPELIEFISGIMTLEAGDIIATGTPPGVGELSVGD
                     EVEIEIEDIGILKNIVVRS"
     gene            454..612
                     /locus_tag="VKL21_02690"
     CDS             454..612
                     /locus_tag="VKL21_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44707.1"
                     /translation="MSDVYDNVWETRKRKPPAREEKKLMEVKPPSTDRDRLNDTIERR
                     KKNVYGMK"
     gene            719..1402
                     /locus_tag="VKL21_02695"
     CDS             719..1402
                     /locus_tag="VKL21_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HMB44708.1"
                     /translation="MPAYNEAKRIEGTVERTAAALGEITSSFEIIIAEDGSIDGTDKI
                     CESLARKYNFVLHLHSEQRQGRGRALNRAFKASRGEILGYIDVDLATDMSHLKELIQS
                     IREGYDFATGSRMLPQSNVKRPFKRGFASKGFNYLTRLMLGSKLYDHQCGFKSFRREA
                     LFSLLEKVKDTHWFWDTELYVRAQRAGYRVKEFPVVWTQGGTTKVNLVKDVFGMGSQI
                     FRLWYEFLW"
     gene            1436..2095
                     /locus_tag="VKL21_02700"
     CDS             1436..2095
                     /locus_tag="VKL21_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44709.1"
                     /translation="MAELGKIEKPEASNFSAKKKLYVVPTLPFEELASQYDLGMKVER
                     FWGEVKEKINYFISTYGNINTLYIEGINDDQKAGQEYLEKPGKENNYYKLLKNLIDGG
                     TVLRGIEKSGRMEKLKLLFEEYSKSFLPEIKEFHMGYYGKDIKFDGWQEYLIKNIQEI
                     QDEMGKSATRILGELPPDSSGVLIITDGRPIEFPQGIDIFQIRPPAFDEIERIIRQMI
                     K"
     gene            2133..2549
                     /locus_tag="VKL21_02705"
     CDS             2133..2549
                     /locus_tag="VKL21_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HMB44710.1"
                     /translation="MTWIIYAVACVFLYGIMQFFIKLSSNGNAITSSLFFVTAQFIAQ
                     LVLGAYFISKSGFDVNSSSIKYSILGGITAAIATILFFLALEEETISKVVPIVNLNVV
                     VGVILGVIILKDEINLRITAGIVLAIISIYLLTSSK"
     gene            complement(2556..3266)
                     /locus_tag="VKL21_02710"
     CDS             complement(2556..3266)
                     /locus_tag="VKL21_02710"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44711.1"
                     /translation="MERIKTGISELDEIIGGFPSNKTILITGNPGSGKTIFGLQFAYT
                     NCLENKKTIYISTEEDASELRLQGKSFGWNLEDLEKKGILKIIELAGYRAMEIKTALS
                     ISMEAVKGDFTQFCDNIPDETEILIIDSLGSHVSNLTAFEFRDRFNLLVYNLAQKGIT
                     TLVLLGGVLSREYVELALYSAYGAVQLMKRENPYTGRRERVMDIVKMRNTKTPVQLLS
                     YEISSRGIIITIPIDSSD"
     gene            complement(3269..>3957)
                     /locus_tag="VKL21_02715"
     CDS             complement(3269..>3957)
                     /locus_tag="VKL21_02715"
                     /inference="COORDINATES: protein motif:HMM:NF037269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HMB44712.1"
                     /translation="LAPEYRKLMDQSINDLLEKREAVFESVHLGKGNKMIHVEVHSRV
                     IEYGGNKVILSVTRDITLRKQAEKMFKENARAELYGFIVSALPVFASGVPSHVRDFLI
                     KNFSERFEKNVKPRFNEEMKQKSLNGDPKQLLDFFMVWISALFLNFGIENKKKTEGKK
                     RVLEFTNCPWINEARGNPIFCFICRSLAKRSFTWTALNGIVEQKSSIASGSKICRFDI
                     LVQGEKLQEA"
BASE COUNT         1207 a          789 c          815 g         1146 t
ORIGIN      
        1 atatgcatgc ttcaatgatg taacggcaag agacctccag aaggaagatg tacagtggac
       61 acggtcaaaa agttttgaca cttttgcacc tgtaggccct tttataaccg accccggatt
      121 ggatgtaaag gatctctcaa ttaaaacaag ggtaaatggt aaactgcggc aggattcaag
      181 aacctccaat ctcatattta gtttgccgga attgatcgag ttcatttcag gtatcatgac
      241 tcttgaggca ggagatatca tagcgaccgg cacaccgcca ggtgtgggcg aattatctgt
      301 cggtgatgag gtcgaaatag aaatagagga catcgggata ttgaaaaaca ttgttgtcag
      361 aagttagaag atagaaatta ttgtatcctc tcatcttatt aagaatgaaa tcaataaaag
      421 taacaaataa caagaaaaaa aggtgaatga agaatgtcgg atgtatatga taacgtatgg
      481 gaaacccgaa agcgtaagcc tccagcaaga gaagagaaaa aactcatgga ggtaaaacca
      541 ccatccacag acagggacag gttaaatgat acgatcgaga gaaggaaaaa aaatgtgtac
      601 gggatgaaat aaggtctgtt catattgaac cgaaaaaaaa ataaatccat ttcatctttt
      661 caagtgatat ttttcttttt aatcaggggg tattattata atagaagttt ctattgtcct
      721 gcctgcctat aatgaagcga aacgaataga gggaaccgtt gaaaggacgg cagccgctct
      781 gggggaaatc acatcgtctt tcgaaataat aattgccgag gatggcagca ttgatggcac
      841 ggataagata tgcgaatcac ttgcaagaaa atataatttc gtgctgcatc tgcattcaga
      901 gcaacgccag ggaaggggac gcgccctgaa ccgcgcattt aaggcatcca ggggagaaat
      961 cctgggatat atagatgttg acctggcaac agacatgtca catctaaaag agctgatcca
     1021 gtctatccgt gagggctatg attttgccac aggctcgcgg atgcttcccc aaagcaatgt
     1081 caagcggcct ttcaaaagag gatttgcaag caagggtttt aattacctta cgcggcttat
     1141 gctgggttca aaactctatg atcaccagtg tggttttaaa tccttcaggc gtgaagctct
     1201 attttcactc cttgaaaagg taaaagatac acactggttc tgggatactg aattgtatgt
     1261 gagagcacag cgcgcaggat acagggtaaa agaatttcca gttgtctgga cacagggagg
     1321 cacaactaaa gtcaaccttg tgaaagatgt cttcggaatg ggatcccaga tattcaggtt
     1381 atggtatgaa ttcttatggt aatgtttata tttcttcatc aataagaggt ataacatggc
     1441 agagcttggg aaaattgaaa aaccggaagc atcgaacttt tcagcgaaaa agaaattata
     1501 tgtggtccct acgcttcctt ttgaagagct ggcttcgcaa tatgatcttg gcatgaaagt
     1561 ggagcggttc tggggcgaag tcaaagaaaa gataaattat tttatttcaa cctatgggaa
     1621 tataaatacg ctctatatag aaggaatcaa cgatgatcaa aaagcaggcc aggaatattt
     1681 ggaaaaacca ggaaaagaaa ataattatta caagttactg aaaaatctta ttgatggtgg
     1741 tacggttctc aggggaattg aaaaaagcgg ccggatggaa aagttgaaac ttctttttga
     1801 ggaatactca aaatctttcc tgccggaaat taaagaattc catatgggtt attatggtaa
     1861 agacataaaa tttgatggat ggcaagaata tctcatcaaa aatatacagg aaattcagga
     1921 tgagatggga aaatctgcaa ccaggatcct tggagaatta cctcctgatt caagtggtgt
     1981 cctcatcatc actgacggaa gacctataga attcccccaa ggaattgata ttttccagat
     2041 aaggccgcca gcttttgatg agattgaaag gattatccgg caaatgataa aataagggaa
     2101 gctttatcct cctttaaacg ctacttaaaa caatgacatg gatcatctat gctgtggctt
     2161 gcgtattcct gtatgggatt atgcagttct tcattaaatt atcctcaaac ggcaacgcta
     2221 taacctcgtc attgtttttt gtaactgcgc aattcatagc gcagcttgtc cttggggctt
     2281 actttatttc aaaaagcgga tttgatgtga attcctccag tattaagtac agcattcttg
     2341 gaggtataac tgctgcaatt gcaactatcc tgttctttct tgctcttgag gaggaaacca
     2401 tttccaaggt cgtgcctatt gtgaatctga atgtagttgt tggcgtgata cttggcgtta
     2461 tcatcttgaa agatgaaatt aacctcagga taacagcagg gatcgttctt gccataatca
     2521 gtatttatct cctgacaagc tcaaaatgag cggcctcaat cggatgagtc aattggaata
     2581 gttatgatta tacctcttga ggagatctca taagacaata gctggaccgg tgtttttgta
     2641 tttctcattt tcacgatatc catcactctt tcccttcgtc ctgtgtacgg attttcccgt
     2701 ttcatgagct ggacagcgcc gtaagcggaa tatagggcaa gttcaacata ttcccttgag
     2761 agaacccctc caagcagcac cagggtcgta atgccttttt gcgccaggtt ataaaccaga
     2821 agattgaacc tgtcacggaa ctcgaatgca gtaagattgg atacatggct cccgagactg
     2881 tcaataataa gaatttcagt ttcatcggga atattgtcgc agaattgcgt aaagtcgcct
     2941 ttgacagcct ccatgcttat gcttaatgca gtctttattt ccattgccct gtatcctgcc
     3001 agctcaatga ttttcaaaat accctttttc tcaagatctt caaggttcca gccaaatgat
     3061 ttgccctgca gcctgagttc cgatgcgtct tcctctgtgg aaatgtatat tgtcttcttg
     3121 ttttccaggc aatttgtata tgcgaactga agcccgaaaa ttgtcttccc acttcccggg
     3181 ttacccgtga taagaatagt cttattcgaa ggaaatcccc caataatctc atccagttct
     3241 gaaatacccg tcttgatcct ctccatattc atgcctcctg taatttttct ccttgaacca
     3301 gaatatcaaa cctgcatatc tttgaaccgc ttgcaatgct tgatttttgc tctacgatgc
     3361 cattcagggc agtccatgtg aaactccgtt ttgcaagact cctgcatata aaacagaata
     3421 tcggattacc gcgggcttca tttatccatg gacaatttgt gaactcaagt actctctttt
     3481 ttccctcagt cttcttttta ttttcaatcc cgaagtttaa aaacaatgca gatatccata
     3541 ccataaaaaa atcgagcaat tgcttcggat caccattcaa agacttttgt ttcatttcct
     3601 cattaaacct tggctttaca ttcttctcaa atctttcaga aaaattttta atgagaaaat
     3661 ctcttacatg agagggaact ccagatgcaa aaacaggaag tgcgctcacg ataaaaccat
     3721 atagctcagc tcttgcattt tctttgaaca ttttttccgc ctgctttcgc agggttatat
     3781 cccgcgtcac gctcagtatt actttatttc caccatattc aattactctg gaatggactt
     3841 caacatgtat cattttattt ccttttccaa gatgaactga ctcaaataca gcttctcgtt
     3901 tttccagcag atcattaata ctctgatcca tcaacttcct gtattcggga gccagaa
//
LOCUS       DATGBN010000123         6043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_514311, whole genome shotgun sequence.
ACCESSION   DATGBN010000123 DATGBN010000000
VERSION     DATGBN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514311"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VKL21_02720"
     CDS             <1..878
                     /locus_tag="VKL21_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44713.1"
                     /translation="KKLFGSKSPFLGLAMQYRLGLIRPMNILRELSKKMDATQAIELA
                     PYLADAWTIPFFVQENDSEKTIYDLLKYSKFTIPSLVGEVFTEEEREFTKVYEAILRL
                     IGAGELDYKNIASILAGRKVIGRADSSLIIPYIKNMEAMGLVESLPLFSSKKKMYRLS
                     SPIMEAFFYLADRYGFEDRDVSFEEARPAIEKLRNLAVQNFIADLFASTYNGKKEYYM
                     TPSKEIDFIITVRNKAVLVGEVKWGKYDTNDVKKFMEKSNFIKAEKIFITQKKKETKI
                     DNVKIMDVDDILAMS"
     gene            959..1999
                     /locus_tag="VKL21_02725"
     CDS             959..1999
                     /locus_tag="VKL21_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HMB44714.1"
                     /translation="MANVAIIGSEKSGRTSLAGKLGKKGTESDITLYNFVKGDRIYSF
                     VDANGYPSSLKTLIQAINLSDIALICVPSSGLNAQVGECIIALDLLGNKRGLFVITMS
                     DKSNPNAISELSDKIKAIAKSTNLENWEIIAVSNTTFEGLESLKEKIFALGEEARTAN
                     STRNELPPRIIVDHFFNVTGMGCVVLGIIAQGTIKEHDKLTLYPTERPAEIRSIQSND
                     VDMKSAPAGTRVGLALKGVQSKEFDRGYLISQKEEISSKFNLKCHLTKFTPGLGVGDA
                     VHLFAGLQDIPATITGMTIGGKDATQAPVGSDCNVLLTTQKNIAYSKNDRLLIVQLNN
                     PRQRLVAGCDIA"
     gene            2011..2796
                     /locus_tag="VKL21_02730"
     CDS             2011..2796
                     /locus_tag="VKL21_02730"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: protein motif:HMM:NF009321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HMB44715.1"
                     /translation="MTQSRYFNLCNDVAAEIKMAVSGLIGKPEAGVTLKIGADGTPTE
                     KIDQVAENAALSVLEADGRSMLFVSEELGEKRIGGKPEFTFVLDPVDGTFNAVNNIPF
                     FCVPIAIGNSNLSDIRYGFVRNLVNGDIYSAEKGKGAFRNDMKIHVSGISELSKLSVL
                     SYSHRPHAVLINNHSVRRVRVFGCAGLELCYVASGIFDAFFDMRGMLRVTDIAASKLI
                     VEEAGGKITDEKGKLLNTPLDVKKRVNIIASNGIAHDKLLELV"
     gene            2812..3633
                     /locus_tag="VKL21_02735"
     CDS             2812..3633
                     /locus_tag="VKL21_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HMB44716.1"
                     /translation="MRKIGIISRCDRDEIVSLVEKIVSSLKSRVEVLIDPKTAQKLHL
                     TGTKMADMRKNGAEFIISIGGDGTVLRGLQKMDDPLPILGINMGTIGFLVDVLPEDSL
                     SVIDKVLSGFEVEERSRLSIMVNDEILPCATNEVVILTANPAKMLSYRIFVDNSFLEE
                     LRADGAVFATPTGSTAYAMSAGGPIIDPRVDGTVIVPLAPFKLSSRPWVVPALSEIKV
                     ELTVPKKEAVLVIDGQYTRHIKEDDTIIITKADKPARFVKTREDGFYEKVRSKLA"
     gene            3761..5041
                     /gene="thiC"
                     /locus_tag="VKL21_02740"
     CDS             3761..5041
                     /gene="thiC"
                     /locus_tag="VKL21_02740"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMB44717.1"
                     /translation="MSLIVEARKGFITEEMKKVADTEGVEPEFVRRGIESGRIVIPVS
                     PYRYTKPCGIGKGLRTKVNASIGTSSDIVDIDMEIEKAKIAEATGADTLMELSTGGDF
                     YDIRKKVIGSTTLSVGSVPLYQAFIEAIRKYGSIVDMKEDDLFKITAGQAKLGTNFMA
                     IHTGINLFTVERLKKQGRFGGLCSRGGAFMTAWMLHNEKENPLYSEFDYLLEIMKEHE
                     VTLSMGNGMRAGAIHDSTDRAQIQELIINSELSDISHEAGVQTIVEGPGHIPIDEIEA
                     NVILMKRLSGEKPFYMLGPLVTDIAPGYDHIVAAIGASLSSAYGADFICYVTPAEHLA
                     LPNLDDVRQGVITARIAAHIGDMVKNKDRQQDLDMGRARRDLQWEKMYELAIDLKYAR
                     SVRDSRSPNDEEACTMCGNYCALKIVKQNFNFER"
     gene            complement(5063..>6043)
                     /gene="ftsZ"
                     /locus_tag="VKL21_02745"
     CDS             complement(5063..>6043)
                     /gene="ftsZ"
                     /locus_tag="VKL21_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB44718.1"
                     /translation="IGANTDAGHLSTMTRADKIILLGEKLCKGHGCGGYPERGAEAAK
                     ENLDEIKEELKGTNLLFLVAGLGGGSGTGAIPVFASVGKEVGALTVACVTIPFTIEMT
                     RREKAREAIRSLAESCDSIVVIDNSKLREVAGNLPLKEALSVANALVGAFVKNITDTI
                     TQPSLVNLDYADLRAVMERGGISSIGIGEGDGPTRVEKAVSQALSTPLLDITDISTAY
                     GVMIHIVGGEDMTLEEVAVAGEQIMDKIPNTKRVIWGAKVDPELSGRVRVMAVVTGVT
                     CPFLSGIPKHEPAPSIPRDIAKPLRQAMEQMVEALPQEKISRAAVFSKER"
BASE COUNT         1776 a         1216 c         1451 g         1600 t
ORIGIN      
        1 taaaaaagct gtttggtagc aaatcaccat ttcttgggct ggctatgcag tacaggcttg
       61 ggctgataag gccaatgaac atattaaggg agctttcaaa gaaaatggat gcgacgcagg
      121 caattgaact tgccccttat cttgcagatg catggactat tccttttttc gtgcaagaaa
      181 acgacagcga aaagaccata tatgatctct tgaaatattc gaaattcaca ataccttcgc
      241 ttgtgggaga ggtatttacg gaagaggaaa gagagttcac gaaggtatat gaggccatat
      301 tgaggcttat aggagcaggc gaattggatt acaaaaatat tgcctccatt cttgccggca
      361 ggaaagtgat tgggcgggca gacagctctt tgataattcc atacataaag aatatggaag
      421 ctatggggct ggtcgaatcc ctgccgctat tttcctcaaa gaaaaaaatg tacagattgt
      481 cttcacctat aatggaggct tttttctacc ttgctgacag gtatggtttt gaagacaggg
      541 atgtaagttt tgaagaagcc aggccagcta tagaaaaact aagaaatctt gcagtgcaaa
      601 actttattgc agatttgttt gcctcaactt acaatggaaa aaaagaatat tatatgacgc
      661 cttcaaagga gatagatttc ataataaccg taagaaataa ggcggtactt gttggagaag
      721 taaaatgggg aaaatacgat accaatgatg taaaaaaatt catggaaaaa tctaatttta
      781 tcaaggcgga aaaaatattt ataacccaaa agaaaaaaga aacaaaaatt gacaatgtta
      841 agataatgga tgttgatgat attttggcta tgagctaaaa gaaatgtgat gagtagtttt
      901 tcaaatcccc atcaacgcca atttcatata gcttcctaac tattagaaca tagatattat
      961 ggccaacgtt gcaattatag gaagcgaaaa atcaggcagg acaagccttg caggaaagct
     1021 cgggaaaaaa ggcactgagt ccgatatcac actatataat tttgtgaaag gggacaggat
     1081 atattcattc gtggatgcaa atggttaccc gtcttctttg aaaacgctca tacaggcgat
     1141 caatctctcg gatatcgcgc ttatctgcgt tccctcgagc ggtcttaatg cgcaggtggg
     1201 agaatgcatc attgctcttg acctgcttgg taataaacgc ggattattcg tgatcacgat
     1261 gtcggataaa tccaatccga atgcgatatc agaattatcg gataaaataa aagccatcgc
     1321 aaaaagcaca aaccttgaaa attgggagat tatcgcggtt tcgaacacca cattcgaggg
     1381 tcttgagagt ctcaaggaga aaatattcgc gcttggggaa gaagccagga ctgcaaattc
     1441 aaccaggaat gagcttccac caaggattat tgtagaccat ttcttcaatg tcacaggaat
     1501 gggatgcgtg gtgctcggaa taattgcgca gggtacaata aaggaacatg acaagctaac
     1561 tttatatccc actgaaagac ctgcagagat acgttccatc cagagcaacg atgttgatat
     1621 gaaatcagcg cctgcgggga caagggttgg acttgcgcta aagggtgtgc agtcaaagga
     1681 atttgaccgt gggtatttga tctcccagaa agaggaaata tcatcgaaat tcaatttgaa
     1741 atgccatctc acaaaattca caccaggatt gggcgtaggc gatgcggtcc atctgtttgc
     1801 cggactgcag gatattcctg caacgataac gggcatgacc ataggcggga aagatgcgac
     1861 acaggcacct gtgggctctg actgcaatgt tttgctcacg acgcagaaga atatcgcata
     1921 cagcaaaaat gaccgtcttc ttattgtcca gttaaataat cccagacaga ggctggtagc
     1981 gggttgcgat atagcatagg gcttacgaaa atgacacaat cccggtattt taacctgtgc
     2041 aatgatgtgg ctgccgaaat aaaaatggct gtttcagggc tgattggaaa acctgaagct
     2101 ggcgtaacat tgaaaatcgg agctgacgga acgcctaccg agaaaattga tcaggtagct
     2161 gaaaatgccg ctctctcggt gcttgaagca gacggcaggt caatgctatt cgtgagcgag
     2221 gaactgggtg aaaaaagaat agggggaaaa cctgaattta catttgtgct tgacccggtg
     2281 gatggtacat tcaatgccgt gaacaatatc ccttttttct gtgtaccgat tgctattgga
     2341 aattccaatt tatcggatat aagatatggt tttgtcagga atcttgttaa tggagatatt
     2401 tattcggcag aaaaaggaaa aggtgcgttt cgaaacgaca tgaaaattca tgtatccggg
     2461 atctcggagt tgtctaaact cagcgtgcta tcatacagcc atcgtcctca tgcagtactg
     2521 ataaataacc atagtgtcag gagagtaaga gtattcgggt gcgcagggct tgaactctgc
     2581 tatgtggctt cagggatatt cgatgctttt ttcgatatgc gtggaatgct tcgtgtaacc
     2641 gatatagctg cttcaaagct tatagtggaa gaagccgggg gaaaaattac tgacgaaaaa
     2701 ggaaaactgc tcaatactcc attggatgtt aaaaaacggg taaatattat agcgagtaac
     2761 ggaatagctc atgataaatt gcttgaactt gtgtagtgga gttggaaaag aatgagaaag
     2821 atcggaataa tttcaagatg cgatagggat gaaatagtct ctcttgttga aaagatagtt
     2881 agcagtttga aatccagggt cgaggtattg atcgacccga aaactgcaca aaagctccat
     2941 ttgacaggta ccaaaatggc cgatatgagg aaaaatggcg ccgaatttat catatccata
     3001 ggcggcgatg gtaccgtttt acgaggcctc cagaaaatgg atgatcccct tccgatcctt
     3061 gggataaata tgggaacaat tggctttctt gtggatgtgc tcccggaaga ttctctttct
     3121 gtcattgata aagtattgtc gggttttgag gttgaggaac gttctcgcct ctcaattatg
     3181 gtgaatgatg agatactccc ctgtgcaacc aatgaggtag tcatcctgac cgccaatcct
     3241 gcaaagatgc tgtcctatcg gatttttgta gataatagct ttcttgaaga gctgcgcgca
     3301 gatggcgcgg tatttgcaac gccaacgggc tcgacggcgt atgcgatgag cgcaggaggc
     3361 ccgatcattg atccgcgagt ggacggcaca gttatagtgc cgcttgctcc ttttaaatta
     3421 tcatcccgtc cctgggtagt tcctgccctg agtgaaataa aggtggagct gacagttccg
     3481 aaaaaggaag cagtacttgt gatcgatggg cagtacacac ggcatattaa agaggatgac
     3541 acgatcatta ttacgaaggc agataaaccc gcaaggttcg tgaagacaag agaagacgga
     3601 ttttatgaga aagtcaggag taagcttgct taatttcgga atgctgaaat ggttttaaaa
     3661 aatcattatt atccaaatca actttacttg attcaaaatc aattcttctg attgaacata
     3721 tttaagtata agtgccacat ttcgaaaaca ggtaattttt atgagtctta tagttgaagc
     3781 aagaaaaggt ttcattactg aagaaatgaa aaaagtagca gacaccgaag gtgttgaacc
     3841 tgaatttgtg aggcgcggga tagaaagcgg gcggatagta attccagtca gcccctatcg
     3901 ctatacaaag ccctgcggga ttggaaaagg ccttcggaca aaagtgaatg catcgatcgg
     3961 aacatcatcc gatatcgttg acattgatat ggaaatagag aaggcaaaga ttgcagaagc
     4021 gacaggcgcc gatacgctga tggagctttc aaccgggggg gatttttatg atatcaggaa
     4081 gaaagtcatt ggctccacga ccctatccgt tggaagcgtc cccctgtacc aggctttcat
     4141 agaagcgatc cgcaaatatg gctcgattgt agatatgaaa gaagatgacc tttttaagat
     4201 aactgccggg caggcaaagc tgggcacgaa tttcatggcg atccatacgg gaattaacct
     4261 ttttacagtg gagcggctca agaaacaggg tcgattcggg ggtctgtgtt cgcgtggcgg
     4321 ggctttcatg actgcatgga tgctgcataa tgagaaagag aatccccttt acagtgaatt
     4381 tgattatctt cttgagataa tgaaagaaca tgaagttaca ctcagcatgg gtaatggtat
     4441 gcgcgccggg gcgatccacg attctacgga cagggcccag atccaggaac ttattattaa
     4501 ctcggaatta tccgacatat cgcatgaagc tggtgtccag accattgttg aggggccggg
     4561 tcacatccct attgacgaaa ttgaagccaa cgtaatactc atgaagcgtt tgagcgggga
     4621 gaaacccttc tatatgctcg gacctcttgt tacagatatc gcacctggat atgaccatat
     4681 tgtggcagcc atcggtgcat ctctttcaag cgcttatggg gctgatttca tatgttatgt
     4741 tacacctgct gaacaccttg cgcttccgaa tttggatgat gtcaggcaag gggtaatcac
     4801 tgcaaggatc gcggcgcata taggtgatat ggtcaagaac aaagaccgtc agcaagacct
     4861 tgatatggga agagcaagac gtgatctcca atgggaaaag atgtatgagc ttgctatcga
     4921 cctcaaatat gcaagatcag taagggacag ccgctcccca aatgatgaag aagcgtgcac
     4981 gatgtgcggg aattattgcg ctctcaagat cgtgaaacaa aatttcaatt ttgaacgctg
     5041 aaaaaaaagc gggaattatc tcctaccttt ctttgctgaa taccgcagct cttgatattt
     5101 tttcctgtgg caacgcttcc accatttgtt ccatagcctg gcggagaggt tttgcgatat
     5161 ctcttggaat tgatggagct ggctcatgtt ttggtatgcc gctcaggaat gggcatgtaa
     5221 ctcccgtaac cactgccatc acgcgcaccc ttcccgaaag ttcagggtct acttttgcgc
     5281 cccagataac ccgcttagta ttgggaatct tgtccattat ctgttccccg gccactgcca
     5341 cttcctcaag ggtcatgtcc tcgcctccaa ctatgtggat catgacaccg tatgcggttg
     5401 agatgtcggt aatatcaagc aatggtgttg acaatgcctg ggacactgct ttttccacac
     5461 gcgttggtcc atcgccttca ccaataccaa ttgaggaaat tcctccgcgt tccatgactg
     5521 ctcgaaggtc agcatagtca agatttacga gacttggctg ggttatggta tctgttatgt
     5581 tcttaacgaa cgctccaaca agtgcgttcg caacagaaag tgcctctttc agtggtaggt
     5641 ttcccgctac ttccctgagt tttgagttgt ctatgacaac tattgaatcg caactttccg
     5701 caagcgatct tatagcttca cgtgcttttt cacgtcgggt catttcaatt gtaaaaggaa
     5761 tagtcacgca cgcaacagtg agagctccga cttcttttcc cacacttgca aaaacgggta
     5821 ttgcccctgt acctgaacct cctcccaggc ctgctaccag gaaaagaaga tttgtacctt
     5881 ttaattcttc ctttatttca tccagattct cttttgctgc ttcagcgccc ctttcggggt
     5941 atcctccgca tccatgtccc ttgcacaatt tttcacctag cagtattatc ttatccgctc
     6001 ttgtcattgt gctaagatgt ccggcatcgg tattagctcc tat
//
LOCUS       DATGBN010000124         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_552861, whole genome shotgun sequence.
ACCESSION   DATGBN010000124 DATGBN010000000
VERSION     DATGBN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552861"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..996)
                     /locus_tag="VKL21_02750"
     CDS             complement(76..996)
                     /locus_tag="VKL21_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB44719.1"
                     /translation="MLRTRLRDFIVTEDDWIFAVADYCHDGGIRSILRYVPDPEGTRG
                     FDIRYRKMDFDDAFAFMKKARPEWVKDVHIVPWDSVKEILSPNKRLPSIVNKNETVKA
                     IVKTLEKHVPPENMGVTGSLLPGLEIESSDIDFVVYGSSWFTARDIIKREKEKKNPIT
                     QISDEMWHDIYKKRKPDISFDEFLLHEARKGNRGMVGGTYFDLLYVRDWKDIASCLRG
                     TDIGNKTIQATVTNADFAFDSPAIYKIDHPEISYVLSYTHTYAGQALPGERIEARGMV
                     ETLGNMKRLVVGTTREPKGEWIKSLTLLES"
     gene            1225..1431
                     /locus_tag="VKL21_02755"
     CDS             1225..1431
                     /locus_tag="VKL21_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HMB44720.1"
                     /translation="MSEYQEKVAPVKVGESYDVSIDDIAKEGDGIARVEGFVIFVPQT
                     KVGDKVKITVNKVMRKFAIAEKAA"
     gene            complement(1463..>3217)
                     /locus_tag="VKL21_02760"
     CDS             complement(1463..>3217)
                     /locus_tag="VKL21_02760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMB44721.1"
                     /translation="KRDKDTSLIQLQRELSQEFDKEERQLHDSSKNSCSFLHKILKSD
                     TDDFRTWLFGGVRTPEEVADWALSNDACGYELLKRYMKEADLLICNYHHFLNEDIRTN
                     VLGWLDKSLADVITIFDEAHNIESAARSHSSMTLTEFTLKRALDEIEANKETNIPEDV
                     QTLLAVFLDTIRSTYNIIFDKKFGERERVGKDWYDLRIADPEERTDMFREKVIVTLGK
                     AGIKKPDETIERLRSFGLTIDAFYEKQFNEGKSPIKKICSCLITAVFLSNYVKFSKDK
                     SYYPILNVRRQNSEISGRLELFTCIPRNVTAPLFASVHCAILMSATLAPFETIKATLG
                     ILRQTRELSFGLTFPKEKRLTIAVSVPALFVKDRDSPQTKEIITKVLTDIIEQSDGNV
                     LIFFPSFYEATQYKNRIKCDVPVFLDEVGVSAQQIRDEFFKLGESGKKAVLISYMWGT
                     LTEGVDYKNGRGRTVVIVGVGYPALNDRTRAVESAYDAEFGHGWEYAIEIPTIRKVRQ
                     ALGRVVRSPQDYGVRVLLDGRYTSTSAKRYGKYSVFKFFPEEERAEIIDVEPERVKYS
                     LMNFFNDIRKNAAKTGKK"
BASE COUNT          881 a          694 c          651 g          991 t
ORIGIN      
        1 gggaatttgc tcagataagc ggagaaaaaa caaagggcaa gagtattttt gagaaagtga
       61 gagatcatat ttgaatcagg attcaagcaa agtcagcgac tttatccatt ccccttttgg
      121 ctctctcgtc gtccctacaa caagtcgttt catattgcca agagtttcaa ccatacctct
      181 ggcttcaatc ctctcgcctg gcagcgcctg tccggcatag gtgtgggtat aggacagcac
      241 ataacttatt tcgggatgat ctattttata gatagcaggg ctgtcaaatg caaaatcagc
      301 attcgtaaca gtggcctgta ttgttttatt tccgatatcg gttccgcgta aacatgacgc
      361 aatatccttc caatcacgaa cgtaaaggag gtcaaaatat gttcctccaa ccatacccct
      421 gttgcctttc ctggcctcgt ggagcaggaa ttcatcgaat gagatatctg gcttgcgttt
      481 cttataaata tcatgccaca tttcatcgct tatctgcgtg atgggatttt tcttttcctt
      541 ttcacgtttg atgatatccc tcgcagtgaa ccatgagctt ccataaacga caaaatcaat
      601 atccgatgat tcgatttcaa ggccgggaag aagagagccg gttacaccca tattttcagg
      661 aggcacatgt ttttcaagtg tttttacaat ggcctttact gtttcatttt tatttactat
      721 cgatggcagc ctcttgttgg gagaaagtat ttctttaaca gaatcccatg gtacgatatg
      781 gacatccttt acccattcgg ggcgggcttt tttcatgaac gcgaaagcat cgtcaaaatc
      841 catttttcga tatcttatat caaaacctct tgttccctcc gggtcgggta cgtacctgag
      901 gattgagcgg attccgccat catggcagta atcagcgact gcaaatatcc agtcatcttc
      961 tgttactatg aaatcccgaa gtcgtgtacg aagcatcctt gactttaggg gataggggtt
     1021 atataaagga tattatttgc agtttcatcc tggtttttgg atttgatttt atttttagca
     1081 aagtcttata taatctaatg acagtaacaa atatagatta caggctgttc cgggatcagc
     1141 atttacagca gtgaaagttc attatatttt ctgcagttat gtttgcccta gcagacgata
     1201 atcgaatgta aggatgtgta aaacttgagt gaatatcagg aaaaagttgc tccggtaaaa
     1261 gtcggagaaa gttatgatgt atcgatagat gatatagcaa aagaaggcga cggcattgca
     1321 agagtagagg gtttcgtcat ctttgtgccg caaacaaaag tcggcgataa agttaagata
     1381 acagtcaata aagtaatgcg caaattcgcg attgcagaaa aggcagcata atataatttt
     1441 ttttagcttt ttcttttaat tattattttt taccggtttt tgcggcattt tttcttatat
     1501 cattaaaaaa gttcataagt gaatacttga cccgctcggg ttctacgtct atgatttctg
     1561 ctctttcttc ttcagggaaa aatttgaaaa cagaatattt accataacgc tttgcggatg
     1621 tggaagtata acgcccgtca agcagcactc gaaccccata atcttgcggt gaacgcacaa
     1681 cgcgcccaag agcctgcctt actttcctga tagtcgggat ctcgatggca tattcccagc
     1741 catgcccgaa ttcagcatca tatgctgatt caactgcacg ggtcctgtca tttaatgcag
     1801 gatatccgac acccacaatg accacggtcc tccccctgcc atttttgtaa tcaacacctt
     1861 ccgtaagcgt tccccacata taggatatta acacggcctt cttccccgat tcgcccagct
     1921 taaaaaattc atccctgatt tgctgcgcgg aaacgccaac ctcatcaaga aaaaccggga
     1981 catcgcattt gatcctgttc ttatactggg tcgcttcata aaagctcggg aaaaatatca
     2041 atacgttgcc atctgattgt tcgattatgt ctgtaaggac ttttgtgatg atttcttttg
     2101 tctgtggact gtccctgtct tttacaaaaa gggccgggac agatacagct atggtaaggc
     2161 gcttttcttt cgggaaagtc aggccgaaag ataattcacg cgtctgcctt aaaataccaa
     2221 gtgtagcttt aattgtctca aaaggcgcaa gtgtcgcaga cataaggatc gcgcaatgga
     2281 cagaggcaaa taatggcgct gtgacatttc ttggaatgca ggtaaatagt tcaagccttc
     2341 cggatatctc ggaattctgg cgcctgacat tcaatatcgg ataatagctc ttatcttttg
     2401 agaattttac gtaattggaa agaaagactg ccgtgatcag gcagctgcat atttttttta
     2461 tcggactttt accttcattg aattgttttt catagaaagc atcaatcgtt aatccgaaac
     2521 tcctcaaacg ctcgatggtc tcatctggct ttttgattcc tgctttccca agcgtcacta
     2581 taactttttc ccggaacatg tcggtacgct cctctggatc cgcgatccgc agatcatacc
     2641 aatccttgcc cactctttcc ctttctccga actttttatc aaaaataata ttataagttg
     2701 atcttattgt atcaagaaaa accgcaagta atgtttgaac atcctcggga atattagtct
     2761 ccttattagc ctcgatctca tcaagcgctc tttttaaggt aaattcagtg agtgtcatgg
     2821 atgaatgaga acgagcagca gattcaatat tgtgggcttc atcaaagatc gtgataacat
     2881 cggcaaggct tttatccagc caaccaagca cattagtgcg gatatcttca ttaagaaaat
     2941 gatggtaatt gcagatcaaa aggtcggctt ctttcatata gcgtttgagc aactcatacc
     3001 cgcaggcatc attcgataaa gcccagtctg ccacctcttc aggtgtgcgc acaccaccga
     3061 agagccatgt gcggaaatca tcggtgtcac tttttagtat tttatgaaga aaggaacagg
     3121 agtttttgct tgaatcatgg agctgccttt cttctttatc aaattcctgg ctcaattccc
     3181 tttgaagttg tatgaggctt gtgtccttat ctcgttt
//
LOCUS       DATGBN010000125         6698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_242673, whole genome shotgun sequence.
ACCESSION   DATGBN010000125 DATGBN010000000
VERSION     DATGBN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242673"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="VKL21_02765"
     CDS             complement(<1..1441)
                     /locus_tag="VKL21_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44722.1"
                     /translation="MNCHKNDFDSIEPESSISNTRGTVSKAGEGGGTDCVSCHEMGGS
                     GATKHIDVSAIKQGVHGNLNNNTANSTFLSDLVDKACWACHGNGTEPSGQHPDNYATP
                     YYCENCHNRTYNLTSTDVSRIPDLTTRKVSEHIQPPYFEGISSTINNSNANCEGCHDK
                     SKVTFSDSGLSRAADVSHYASRTDLVKPSINCSLCHKNSQNASAYWANLIRHPAKSQN
                     DSFCDNCHNTSLATDLHSRPLVKPNNIHNGFDWQNNDHINDPAESSEACLSCHGGGHN
                     TGYSLCEDCHIENGKGPLSSWRDANETTPRVYAHTNFSTVINVPNQSLIYPPSPSART
                     FSSCYSFNSNTLEGTCHGNSYKNLTASGGFYAFKRISSDSRSTPYHFTQTIDRLPDTT
                     NCVFCHNQTDAVIRKAWGNAAQITSGRHSWYTGSNNSKCWSCHVNTGTVPMDFHSESV
                     TAGGGSDCISCHSPNDVNTSKFARHANLDT"
     gene            complement(1516..2508)
                     /locus_tag="VKL21_02770"
     CDS             complement(1516..2508)
                     /locus_tag="VKL21_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HMB44723.1"
                     /translation="MKAVRVSFEESESVRKKLLYGSSLDRTRKLVKKNGFIEIPVTES
                     FDTYEFTLVEQDKPEYYLQKKTLDRILNIPESDKKLLPRGWNILGDIIIVTLNENLEH
                     RRKEIGEALLCLYPGCKTVLLDRGISGQMRLPVREIIAGNGTETIHKERGCLFMLDAM
                     RIMFSQGNLAEKERMSKLGRDETVVDMFAGIGYFSIPMAVHSKPKKIISIEINPVSFC
                     YLKENIKLNKVGNIVEPISGDCSKLTPRSMADRVIMGYLDGQEYLPHGINALLPGGIL
                     HYHEAVPEAVESRPVERIMEASRRQGRHAQIMSVRRIKKYAPGVWHVVVDATIK"
     gene            complement(2505..3503)
                     /locus_tag="VKL21_02775"
     CDS             complement(2505..3503)
                     /locus_tag="VKL21_02775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HMB44724.1"
                     /translation="MKTVRISIIGFGAVGQGVARSILSKRKYLNKQGIDLCVVGISDS
                     RGSEINDRGIDIENALLRKKQKGSVCAGTRNALDIIGNVEHDIVVETTPTNINNGEPG
                     LSNMRLAFKTGKHIVTSNKGPLALHFNELKEAAQDNGLFFRYEATVGGAVPIFNLVHE
                     ALAGNTVVGIEGILNGTCNYILTRMSEERMPYDLVLKEAQELGIAETDPTYDVEGIDS
                     AIKLVIIANSIFGLDSTYKDVDVTGITKITPEALELANRNNYVVKLVCEAREGKLTVA
                     PRLVLKRHPLAVGGTLNVASIVTDLAGRITISGKGAGSIETASSILSDILYIIKNS"
     gene            complement(3500..4000)
                     /locus_tag="VKL21_02780"
     CDS             complement(3500..4000)
                     /locus_tag="VKL21_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HMB44725.1"
                     /translation="MTLELKDIPGQLVLALSPISEFHGNIMSVIHQHDEKTPRGTIPV
                     QITFEIEHRVLEKLIVRLENSGVKVARVGEERLRSTVSVILIGHIVHSDMGDTIDKID
                     STGFAEVVDMSLSMPAVEKTSSAYLVINAVGENELGKALDIMREVAIKKDLLMIEPIR
                     LNSVGA"
     gene            complement(4104..4244)
                     /locus_tag="VKL21_02785"
     CDS             complement(4104..4244)
                     /locus_tag="VKL21_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44726.1"
                     /translation="MEIIEVKCRNCSKEIYIRSEQVREKMFCTLGCMNSYEVPSQEKD
                     FI"
     gene            4467..4724
                     /locus_tag="VKL21_02790"
     CDS             4467..4724
                     /locus_tag="VKL21_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44727.1"
                     /translation="MAETLLENVLSFIYTIGHWIGAKIVELIQYVSGILIPSSVVDAI
                     GMLVILTIFLAIAEVAKKAIWIVVVIGWVFIIIRILMLMIG"
     gene            complement(4729..5622)
                     /locus_tag="VKL21_02795"
     CDS             complement(4729..5622)
                     /locus_tag="VKL21_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HMB44728.1"
                     /translation="MKCQRCSKSAIIYQKYSNAHLCKSHFIEDVERKIKRDIRKFKMV
                     GRYEKIAVALSGGKDSIALLYILHKVFRNRPDLEFIAITIDEGIKGYRENTIEHAIKL
                     TGELGIRHIIRSFEDEFKTSLDDLTKKKENRACTLCGVLRKNILNKTARELGATKIAI
                     GHNLDDESQTILMNYMRGDIDRLKRMIPGTIQKGMVPRIKPLRSIPEKEVALYGFINS
                     LPVSMDECPYAGFALRNEIRELLNNYEVNHPGTKYSLLGGYESISEFLRPTDKQIMFC
                     EKCSEPGSERICKTCRLLGRN"
     gene            5737..5928
                     /locus_tag="VKL21_02800"
     CDS             5737..5928
                     /locus_tag="VKL21_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44729.1"
                     /translation="MAGIAIKTFVIVFFLVLMNFTIVTGKSEIICPGYLKTPEKSNFS
                     YLFFTAKMVPMGKHIFSLT"
     gene            complement(6058..>6698)
                     /locus_tag="VKL21_02805"
     CDS             complement(6058..>6698)
                     /locus_tag="VKL21_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44730.1"
                     /translation="PIDHPMMFPPVNHAGEAACVACHAGVVFPPSSPFAGQPVTCAIC
                     HIDPATKNEHPAGMAPPTTAAGEVSCTNCHIQTGHPTILCANCHPDPWPAATPTPAPT
                     PTPVPTATPTPTPTPVPGQKADVTFKVTDSATGKPIENAIVLMDGIKIVTNDHGIAVF
                     KMVAFGKHTYSVTKEKYLKAEGKINVKGKTTVYVKLKKITREDHDHNNEEHN"
BASE COUNT         1707 a         1448 c         1347 g         2196 t
ORIGIN      
        1 ttgtgtccag gttggcatga cgtgcgaatt tggaagtatt aacatcattg ggagagtggc
       61 atgaaatgca gtcgctaccc cctcctgctg ttacactttc cgagtggaaa tccattggca
      121 cagtgcctgt gttgacatgg cagctccagc acttgctatt gttgcttcct gtataccagc
      181 tgtgtcttcc ggaagtgatc tgggccgcat tcccccaggc ttttcttatt acggcatcgg
      241 tctggttgtg acagaacaca caatttgttg tgtccggcag tctgtcgatc gtctgggtga
      301 aatgataggg ggtactcctt gaatcagatg atatgcgctt aaatgcataa aagccgccgc
      361 ttgcagttaa attcttatag gaattgccgt ggcatgttcc ttcaagcgtg ttactgttaa
      421 acgaataaca tgaagaaaag gttcttgcag atggtgaagg aggatatatc agggattgat
      481 taggcacgtt aataacggta gagaaattgg tatgagcata tacacgcggg gttgtttcat
      541 tagcgtccct ccatgaagat aagggacctt taccgttttc tatatggcag tcttcacata
      601 atgaatatcc tgtattgtgg cccccgccat gacatgaaag acatgcttca ctactctcgg
      661 ccggatcgtt aatgtgatca ttattctgcc agtcaaatcc gttgtggata ttattaggtt
      721 ttacaagtgg tctactgtgt aagtccgttg cgagacttgt attatggcaa ttgtcgcaaa
      781 atgaatcatt ctgtgatttt gcaggatgtc tgatcagatt cgcccagtat gcgcttgcat
      841 tttgtgaatt cttatggcac aagctacaat taatgctcgg tttaacaagg tcggttcttg
      901 atgcataatg agaaacatca gccgcacggg aaagaccgga atccgaaaac gttactttac
      961 ttttgtcatg acaaccttca cagtttgcat ttgagttatt tatcgttgaa gatattccct
     1021 caaaatatgg tggctggata tgctcggata ctttcctggt agtgaggtca gggattctac
     1081 ttacatctgt tgaggtcagg ttgtatgtcc tattatggca attctcgcaa taataaggtg
     1141 ttgcatagtt gtcaggatgc tggcctgatg gttctgtacc attgccatga catgcccagc
     1201 atgctttatc taccagatct gagagaaaag ttgaattcgc agtgttgtta ttcaggtttc
     1261 catggacacc ctgctttatc gctgaaacat caatgtgttt ggttgcaccc gaacccccca
     1321 tttcatgaca tgaaacgcaa tctgtgccac ctccttctcc agcttttgat actgttcctc
     1381 ttgtattcga tattgaactt tccggttcta ttgagtcaaa atcgttctta tgacagttca
     1441 tgcagtcatt tcctgtacca gcaggatgaa tttctgatgt tacaattcaa tttgtttcaa
     1501 tgtgtatata tttttctatt tgatagtagc atcaaccacc acatgccaga ccccgggcgc
     1561 atatttcttg atccttcgca cactcatgat ctgcgcatgc cttccctgtc tccttgaggc
     1621 ctccattatc ctttcaacag ggcggctctc cacagcttcc ggcacagcct catgataatg
     1681 cagtatccca cctggcagca gggcgttgat cccgtgtgga aggtattcct gtccatccag
     1741 gtatcccata atgacacggt ctgccatact tcgaggagtt agttttgagc agtcccctga
     1801 gatgggctct acgatattcc ccactttatt gagtttgata ttttctttta aataacaaaa
     1861 cgaaacaggg tttatttcaa tggaaattat tttttttggt tttgagtgaa cggccattgg
     1921 tattgaaaaa tacccgatcc ctgcgaacat atcaacaacg gtttcatctc ttcccagttt
     1981 gctcatgcgt tctttttctg cgagatttcc ctgggagaac atgatccgca tggcatcaag
     2041 cataaaaagg cagcctcttt ccttatggat tgtttcagta ccattccctg caatgatctc
     2101 acgcacaggc agcctcattt gtccgcttat ccccctgtca agaagcacgg tcttgcatcc
     2161 gggataaagg cagagtaatg cttcacctat ctctttcctg cgatgctcca gattttcatt
     2221 aagtgttaca attattatat cgccaaggat attccatccc cggggtaata attttttatc
     2281 actttccgga atattaagga ttctgtcaag tgtttttttc tgtaaataat attcgggctt
     2341 atcctgctca acaagggtaa attcatatgt gtcaaatgat tccgtgacgg gaatctcgat
     2401 aaaaccattc tttttcacta gcttcctggt tctatcgaga gatgacccat aaagaagttt
     2461 tttacggact gattctgatt cctcaaaact tacgcgaaca gccttcatga atttttaata
     2521 atatagagga tatcgctaag gatcgagctt gcagtttcga tcgaacctgc acctttgccg
     2581 ctgatagtga tccttcctgc aaggtcagtt actattgacg caacgttcag cgtgcctccc
     2641 accgcaagcg ggtggcgctt tagaaccagt cgcggtgcaa ctgtaagttt gccctccctt
     2701 gcctcacata cgagtttgac aacataatta ttccgattgg caagttcaag agcttcaggt
     2761 gtgatctttg taatgcctgt gacatcaacg tccttgtagg tggaatccag cccgaaaatg
     2821 gagtttgcaa ttattacaag cttgatagca gagtctatac cttcaacatc atacgtggga
     2881 tctgtttcgg ctattcccaa ttcctgcgct tcttttagca caagatcata tggcattctc
     2941 tcttcggaca ttcttgtaag tatgtaattg catgtgccat tgagtattcc ttctatccct
     3001 acgacagtgt ttcctgccag agcttcatga acaaggttaa aaatcggaac agcgccccca
     3061 acggttgctt cgtacctgaa gaaaagccca ttgtcctggg ctgcttcttt gagttcgttg
     3121 aaatgcaatg caagaggacc tttattggaa gttacaatat gctttcccgt tttaaacgcc
     3181 aaccgcatat tacttagtcc cggctctccg ttattgatat ttgtaggggt ggtttcaaca
     3241 acaatatcat gttctacatt acctataata tcaagtgcat tcctggtccc tgcgcagaca
     3301 gaaccttttt gtttttttct caaaagggcg ttttcaatat caatgcccct gtcatttatt
     3361 tcactgcctc ttgaatcgga tatccccaca acgcacaggt caattccctg tttattcagg
     3421 tatttccgtt ttgataggat cgaacgcgcc accccctggc ctacagcgcc aaagccgatg
     3481 attgaaattc ttacggtctt catgctccca ccgaattcaa gcgaattggt tctatcatga
     3541 gaaggtcttt cttaatcgca acttctctca tgatatcaag cgctttgcct aattcattct
     3601 cgccaacggc attaattaca agataagctg aagatgtttt ttcaacagcc ggcattgaaa
     3661 gcgacatgtc aacgacttcc gcaaagcctg tcgaatcaat tttatcaatc gtatctccca
     3721 tatcagaatg aacaatatgg cctataagaa tgacagaaac ggttgatctg agtctttctt
     3781 ctccaaccct tgccactttt acgccgctgt tttcaagcct cacgataagt ttttccaata
     3841 ctctgtgctc tatctcaaat gttatctgga ccgggattgt accgcgcggg gttttctcgt
     3901 catgctggtg gataacgctc atgatattcc catgaaattc cgatattggc gagagcgcca
     3961 gcaccagctg cccggggata tccttaagct caagggtcat cgaaacgatc atgtccccat
     4021 ctatgcacag gtgattaata agttttttta gtgaatgaat tttccattgg aaatattaaa
     4081 aaaataacag aaaaaaagaa ccgtcagata aagtcttttt cttgagaagg gacttcatac
     4141 gaattcatgc acccaagggt acagaacatt ttttccctga cttgttcact ccttatatat
     4201 atttccttgc tacaattcct gcatttgact tcgattattt ccatgttgca ttctcctatt
     4261 attcattttg atatatttca ggttacagaa aatctagaaa aatcctgttc ccaaaacaaa
     4321 tctgcctgaa gatacctatc aacttattct aataccactg aattagctgt attggtgtta
     4381 aggtttaaat gtttttgccc aaacttagcc agatgaggca atacttatat agtttataaa
     4441 ctgttgttat ctgcttgagg taaatcatgg cagaaacatt attagaaaac gttctttcat
     4501 tcatatacac gattggacac tggataggcg caaagattgt tgagcttatt caatatgttt
     4561 ctggtatctt gatcccgtca tctgttgtgg atgccatagg gatgctcgta attcttacaa
     4621 tattcctggc aatagcggaa gttgccaaaa aggcaatctg gatcgttgtg gttatcggat
     4681 gggtattcat aataatccgt atattgatgc ttatgatcgg ctgaatattc aattccgccc
     4741 aagcagcctg caggtcttgc atatcctttc actgcctggc tccgaacatt tttcgcagaa
     4801 catgatttgc ttatcagtcg gacgtaaaaa ttccgaaatt gattcataac cgcccagtaa
     4861 agagtatttt gtaccggggt gatttacctc ataattatta agcagctccc ttatctcatt
     4921 tcgcagggca aagcctgcat atgggcattc atccatactt acgggaaggc tatttatgaa
     4981 tccataaaga gccacctctt tttcgggaat actgcgaagc ggcttgatcc tgggcaccat
     5041 tcctttctgg atagtccctg gaatcattct tttcaacctg tcgatgtccc cgcgcatgta
     5101 attcattaaa atcgtctggg attcatcatc aagattatgc cctattgcga tcttagttgc
     5161 cccgagttcg cgcgcggttt tattcaggat attttttcta agcacaccgc acagggtgca
     5221 ggcacgattc tctttttttt tagtcaaatc gtcaagtgat gttttaaact catcctcaaa
     5281 agacctgata atatgccgga tcccaagttc acctgtgagt tttatagcat gttcaattgt
     5341 attttcacgg taacccttta ttccttcatc tatcgtaata gctataaatt caaggtcggg
     5401 ccgattccgg aataccttat gaagaatata taataaagcg atgctgtcct ttccaccgct
     5461 taatgctacg gctatctttt catatctgcc taccattttg aatttgcgaa tgtcgcgctt
     5521 gatctttcgc tcaacatctt cgataaaatg agatttgcaa aggtgagcgt tagagtattt
     5581 ctggtatata attgcgctct tgctgcatcg ctggcatttc ataattcaag tttaatacca
     5641 ttcttttatt aatatctata ggggccaaaa ctctcacacg ttatgtaacg acccgttcct
     5701 gtcttttatt gttaccggaa caataggagg gaatccatgg caggaattgc aatcaaaact
     5761 ttcgttattg tatttttttt ggtactcatg aactttacta tcgtgacagg taagtctgaa
     5821 ataatctgtc ctggatatct gaaaactcct gaaaagtcta atttttcgta tttgtttttt
     5881 acagcaaaaa tggttccaat gggaaagcat atattctcat taacataata aaagtttatt
     5941 cttattttat ttatacttaa aaacacagaa atttcttgga ttttatgaaa aacttgaaaa
     6001 aataaggaag aggttagaat tttttatgat cctaatctct ttttttgaag atatctgtta
     6061 gttatgctct tcgttattat gatcgtggtc ttccctggtt attttcttca attttacata
     6121 aacagttgtt ttacctttta cattaatttt tccttctgcc ttaagatatt tttccttagt
     6181 tacgctatat gtgtgttttc caaacgcaac catcttgaag actgcaattc catgatcgtt
     6241 tgtaacgatt ttgatgccgt ccattaaaac aatggcgttt tctatcggct tacctgttgc
     6301 gctatcagtt actttaaaag tcacatcggc tttttgtcct gggacaggtg ttggagttgg
     6361 ggttggtgtt gctgttggaa ccggtgttgg tgtgggtgct ggtgttggag ttgctgctgg
     6421 ccatgggtca gggtgacaat ttgcacataa tatagtagga tggccagtct gtatatgaca
     6481 atttgtacac gatacttctc ctgctgctgt agtcggtggc gccatacctg ctgggtgttc
     6541 attttttgtg gccgggtcaa tgtgacatat tgcgcatgtt actggttgac cggcaaatgg
     6601 tgaactaggt ggaaatacta cacctgcatg acatgctaca caagctgctt ctcctgcatg
     6661 gtttactggt gggaacatca taggatgatc tattggtg
//
LOCUS       DATGBN010000126         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_9453, whole genome shotgun sequence.
ACCESSION   DATGBN010000126 DATGBN010000000
VERSION     DATGBN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9453"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VKL21_02810"
     CDS             complement(<1..205)
                     /locus_tag="VKL21_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HMB44731.1"
                     /translation="MIKTTILRTKKPRLKNPVLIEGLPGVGHVGKLVAEHMVEELGAK
                     KIMEIYSPHLPPQVIVEHDGTVRL"
     gene            complement(202..360)
                     /locus_tag="VKL21_02815"
     CDS             complement(202..360)
                     /locus_tag="VKL21_02815"
                     /inference="COORDINATES: protein motif:HMM:NF009623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HMB44732.1"
                     /translation="MVSKIKKCTCGRYTLKEVCPVCGSPAKYSRPARFSLEDRYGKYR
                     RITKKVES"
     gene            complement(382..1182)
                     /locus_tag="VKL21_02820"
     CDS             complement(382..1182)
                     /locus_tag="VKL21_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HMB44733.1"
                     /translation="MERSGWPDEGDNVVCSVKRVTDFGAFVELDEYGHKEGLIHISDV
                     ASGWVKYIRDHVREGQKIVCKVLYVDTAKHHIDLSLKDVNEHQRREKIQEWKNEQKAE
                     KWIEYVAKTVKISAEELPKLTDLFYDKFGSVYAAFEKSRFEGTSILTKIGVGTEVADA
                     ITKVAHDNLKKPQVEISGYIDLTCYLPDGIEHIKKALGIANKVDNDEFNVNISYVGAP
                     RYRIHVIAPDYKKAEGVLKKAAQSAIDIIEKAGGSGEFHRYSEQKAGS"
     gene            complement(1182..1355)
                     /locus_tag="VKL21_02825"
     CDS             complement(1182..1355)
                     /locus_tag="VKL21_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HMB44734.1"
                     /translation="MEPKSRFLKVKCNDCENEQVIFGCASTAVTCLVCGRTLSEPTGG
                     KAFVKTQIVEVLE"
     gene            complement(1358..1642)
                     /locus_tag="VKL21_02830"
     CDS             complement(1358..1642)
                     /locus_tag="VKL21_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HMB44735.1"
                     /translation="MKIPKNFNTYCPSCKTHTPHVVERVKKGQASTLTRIVRQKYRHI
                     GIGNSGKFSKVPGGDKPTKRINLRYRCKTCKKAHQKPKCFRAGKFEIVES"
     gene            complement(1648..2397)
                     /locus_tag="VKL21_02835"
     CDS             complement(1648..2397)
                     /locus_tag="VKL21_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44736.1"
                     /translation="MPEQSRINLENILRVEREERKKLVPLEEDFYGKVAGQIHDLEEE
                     KRKIDDPYSTKHAIIEDEQKTVKNAIESIIYKRTTKIINEARYNAEMTLHETSKQKEK
                     HNLDSMTHEERIFYNRLLEVMTEWRSEVVNNIFKRTHHQTAILPEEDKLPEILPPEKN
                     DINKEPPEKKDDSNKEPPEKKDDSNKEHSDKKDISKEYIVVRLLKDIPTFVGVDGRNY
                     TFAKEDVAVLSTINAKALINRKAAIQIMVKR"
     gene            complement(2390..3790)
                     /gene="priS"
                     /locus_tag="VKL21_02840"
     CDS             complement(2390..3790)
                     /gene="priS"
                     /locus_tag="VKL21_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HMB44737.1"
                     /translation="MNFRTKQYLRKRFADYYQNSKLAMPHDFTRREWGFIFFDELPEV
                     VMRRHKAFSRENEALDYIRAMVPAHAYHSAAYYEFPGAVTMKEKKWQGADLIFDLDAD
                     HLPGKVRSYAEMLSNVKSETAKLLDFLLQDFGFLENNISIVFSGGRGYHIHVRDPKVL
                     TLESPQRREIVDYVGGTGLSTDFLFKPAKHKVYDEGKFKKKELDSARKIASFEDRPGG
                     FGWGKRISKYLVQFLKDISIKDEKVAQDEIADLMRNYYLKKPNYDTIPEDIAQQIIRM
                     KDENRKIGIEKIAQEVGINKNRVRNFLMTKDLLDHKREATMILKKIRNMSDSPETFDR
                     IENQGIIDESFIFDAIVQKAIEENSISLGSAHTDEPVTADIRRLIRLPSSLHGGSGMR
                     VVPLSLSEFGNFEPLNDAVVFSEKMVDIEIISPLRPQNSKVEMKGKSFTVTEGKNTLP
                     EYAAIYLMCRGAAEYA"
     gene            3920..4732
                     /locus_tag="VKL21_02845"
     CDS             3920..4732
                     /locus_tag="VKL21_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMB44738.1"
                     /translation="MLIVTCVKQVPDTTQVKVDPVTGTLIREGVPFIINPFDTYALEE
                     SLRLKEKYGFRVAVISMGPPNTEVTLRKALSLGADEVILLSDRAFGGADTLATSLVLA
                     EAIKKLGQTQEVAMVICGKQTIDGDTAQVGPGIATRLGYSQLTLVDHIENVDLKAKKI
                     VVRRRLEGRHEIVQAPLPAMISVLREINRPRYPTVPMRLFAQDAPVTLWNNKAMGLKE
                     DTIGLKGSATQVRKIFSPHRESGEIIGDGIKNPIAAAGLLIERLKEKNILCL"
     gene            4766..>5173
                     /locus_tag="VKL21_02850"
     CDS             4766..>5173
                     /locus_tag="VKL21_02850"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMB44739.1"
                     /translation="MEEEKKIRKPRGIAKLIQGKCIACGARCQSDCPEDAIEMNDKGE
                     PVINLDKCIGCRRCVKICPAQALEMYFTPEEQKILAQIASSSKGKEPQEPIIEEAGIK
                     EYKNVWVFVEHTEGEPATVSWELLGAGAKLASSL"
BASE COUNT         1307 a         1173 c         1047 g         1646 t
ORIGIN      
        1 ccagcctgac agtaccatca tgttcaacaa tgacctgcgg aggcaggtgc ggggagtaaa
       61 tttccattat cttttttgct cccagttcct cgaccatgtg ctcagctaca agtttcccca
      121 catgcccgac ccctggaagc ccttcaataa gtacagggtt tttcaatcgt ggtttcttag
      181 ttctcaaaat agttgtcttg atcatgattc aacctttttt gtaatccgtc tgtatttccc
      241 gtatctgtct tcaagggaaa aacgtgcagg tcgtgagtat ttagcaggag aaccgcacac
      301 tggacagact tccttgagcg tatatctccc gcaggtgcat ttttttatct tggataccat
      361 aactgcaggt ctttaataat attacgatcc cgctttttgt tcactatacc tgtggaactc
      421 accgcttcca cctgcttttt cgatgatatc gatggctgat tgcgctgctt tcttaagaac
      481 gccttcagcc ttcttataat cgggagctat aacatggatc ctgtatcttg gcgcaccgac
      541 ataactaata ttaacattaa attcatcatt atccacttta tttgctatac ccaatgcttt
      601 ctttatatgt tctatcccat caggaagata gcatgtcagg tcaatataac cggaaatttc
      661 aacctggggt tttttcaagt tatcatgggc aacttttgtg atcgcatcgg ctacttctgt
      721 tcccactcct atttttgtca ggatcgatgt cccttcaaat cttgatttct caaatgcggc
      781 atagacgctg ccgaatttgt catagaacag gtcagttaac ttgggaagtt cttcagcgct
      841 tatcttaacg gtcttggcaa catactcgat ccatttctct gctttctgtt cattcttcca
      901 ttcctggatc ttttcacgcc gctgatgctc atttacatcc ttgagcgaga ggtcaatatg
      961 atgctttgcc gtatcaacat acagtacctt gcatactatc ttctggcctt ccctcacatg
     1021 gtcccttata tacttgaccc agccagaagc tacatccgag atgtggatta atccttcttt
     1081 atgtccgtat tcatcaagtt caacgaaagc cccgaaatcg gtaaccctct taacgctgca
     1141 aacaacattg tcaccttcat cgggccatcc gcttctttcc atcactcaag aacctctact
     1201 atctgtgttt ttacgaacgc tttcccgcct gtaggctcgg aaagtgttct tccgcaaaca
     1261 agacaggtta cggcagtgct tgcgcatccg aaaatgacct gctcgttctc acagtcgtta
     1321 catttaactt ttaagaatct gctctttggt tccataatta agactccacg atctcaaatt
     1381 ttcccgctct gaagcatttg ggtttctgat gtgctttttt gcatgtttta catctgtatc
     1441 tcaggttaat gcgctttgtg ggcttgtccc ctccgggtac ttttgagaat ttgcctgaat
     1501 ttccgatccc tatatgcctg tacttttgtc tgactattct ggtcaatgtc gatgcttgcc
     1561 ccttttttac tctctccact acgtgcggcg tatgggtctt gcatgacgga caatatgtat
     1621 tgaagttctt gggtattttc atgagaatca ccttttaacc atgatctgaa tggctgcctt
     1681 gcgattgatc aatgctttag cattaattgt cgataaaaca gcaacatctt cttttgcaaa
     1741 ggtataattg cgtccatcga ctccaacaaa tgttggaatg tcttttagca gacgcacaac
     1801 aatgtattct ttacttatat cctttttgtc tgaatgttct ttattggaat catctttttt
     1861 ttcagggggt tctttattgg aatcatcctt tttttccgga ggttctttgt ttatatcgtt
     1921 tttctccggt ggaagtattt cgggtaactt atcttcctcc ggtaaaattg cagtttgatg
     1981 atgggttctc ttgaatatat tatttacgac ttcacttctc cattcggtca tcacttcaag
     2041 aagcctgtta taaaatatcc tttcttcatg tgtcatcgaa tcaaggttgt gtttttcctt
     2101 ctgttttgaa gtctcatgaa gagtcatttc agcgttatac cttgcttcat tgattatttt
     2161 ggtcgtacgc ttatatatta tgctctcaat agcgtttttt actgtctttt gttcatcttc
     2221 gataatagca tgttttgtac tgtaggggtc atcgatctta cgtttttctt cttcaaggtc
     2281 atggatctgt cccgcgactt tcccgtaaaa atcctcttcc aggggaacaa gtttctttcg
     2341 ttcctctctt tccactctca agatgttctc aaggtttatc cgggattgtt caggcatatt
     2401 cagcagctcc acgacacatt agatatattg ccgcatattc gggcagggtg ttcttacctt
     2461 ctgtcactgt aaagcttttt cccttcatct caacttttga attctggggg cgaagaggtg
     2521 aaatgatctc gatgtctacc atcttttcac tgaataccac ggcgtcatta agaggctcaa
     2581 aattcccgaa ttcggaaaga gacaatggca cgacccgcat ccctgagccg ccatgaagag
     2641 aggaaggcag tcgtatgaga cgacggatat ccgctgttac aggttcgtca gtgtgcgcgc
     2701 tgccaagact gatgctattt tcttcgatcg ccttttgcac aattgcgtca aaaataaatg
     2761 attcatcaat tattccctgg ttttcgatcc tgtcaaatgt ttcaggacta tctgacatat
     2821 ttctgatttt cttgagtatc atggtagctt cccgtttatg atccagaaga tcctttgtca
     2881 ttaagaagtt tcgaactcta ttcttattta ttccaacctc ctgtgcaatt ttttctattc
     2941 ctatttttcg attttcgtct ttcatccgga ttatttgttg ggcaatatct tcgggaatcg
     3001 tatcataatt gggtttcttt aaataataat tcctcataag gtcggcaatt tcatcctgtg
     3061 ccactttttc atctttaatt gaaatatcct ttaagaattg aaccaggtat ttactgattc
     3121 ttttccccca gccgaatcct cccggcctat cttcaaagga tgctattttt cttgcggaat
     3181 ccagttcttt tttcttaaat tttccttcat catacacttt atgttttgca ggcttgaaca
     3241 agaaatcggt acttaatccc gtacctccca cataatccac gatttctctc ctctgggggc
     3301 tttcaagcgt cagcaccttt gggtcccgca catggatatg atatccccgc cctcctgaaa
     3361 agacaatgct tatgttattt tccaggaatc cgaagtcctg gagcaggaaa tccaggagct
     3421 ttgctgtttc tgatttcaca ttggatagca tttccgcata cgatcgtacc ttcccgggca
     3481 aatggtctgc atcaagatca aagatcaagt ccgccccctg ccattttttt tctttcatgg
     3541 tgactgcgcc cgggaactca taatatgcag ccgaatggta agcatgagcg gggaccattg
     3601 cacgaatgta atcaagtgct tcattttccc gggaaaaagc cttgtgtcgg cgcatcacta
     3661 cttcgggaag ttcatcaaaa aatatgaaac cccattcgcg ccttgtgaaa tcatgaggca
     3721 tggcaagttt tgagttctgg taataatctg caaacctttt tcttaagtat tgctttgtcc
     3781 tgaaattcac acgtttttat tgtcctccag gcttaaaacc ataatgtatc tcctatggtt
     3841 tatgcaataa gttttatagt tgtaaggaga atataatcgg aattaaactt tcaaatattg
     3901 gtactaaagg gatgattaaa tgctaatagt tacatgtgta aaacaggtgc cggatactac
     3961 ccaggttaaa gttgacccgg tcacaggtac tttgatccgt gagggtgtgc cgttcataat
     4021 aaatccattt gatacttacg ctttggaaga aagcctgaga ctaaaagaaa aatacgggtt
     4081 cagagtagct gtaatttcaa tgggtcctcc caatactgaa gttacactcc gcaaagccct
     4141 ttcactggga gctgatgagg tgatactatt gagcgacagg gcatttggcg gagctgatac
     4201 cctggctacc agcctggtcc ttgccgaagc catcaagaaa cttggccaga cgcaagaagt
     4261 ggctatggta atttgcggca agcagaccat tgatggggat acagcgcaag tcgggccggg
     4321 gatcgcaacc cgtttaggat attcacaatt gaccctggta gaccatattg aaaacgtgga
     4381 cctgaaagca aaaaaaatag ttgtcagacg cagactcgaa ggcaggcacg aaatcgtaca
     4441 ggcgccatta cctgcaatga tctcggtgct ccgggaaata aaccgtcccc gctatcccac
     4501 agtgcccatg cgtctttttg cgcaggacgc ccctgtaaca ttgtggaata acaaagcgat
     4561 gggattgaag gaagacacta tcgggcttaa aggctctgct acacaggtcc gaaagatctt
     4621 ttctccccac agggaaagcg gcgagataat tggtgacggg ataaagaatc caatcgctgc
     4681 tgctggatta ttgattgaaa ggctcaaaga aaagaacatc ctgtgtttat aggatcacga
     4741 aatttaatat tgcttagagg cacatatgga agaagagaaa aagatacgca aaccccgcgg
     4801 tatagctaaa ctaatccagg gaaaatgcat agcttgcgga gcacgttgcc agagcgattg
     4861 ccctgaagat gcaatagaaa tgaacgataa gggtgagccg gtcatcaatt tggataagtg
     4921 catcggatgt cgaaggtgcg tcaagatttg ccctgcccag gccctggaga tgtattttac
     4981 tcctgaagaa caaaagatac ttgcgcaaat agcttcttct tcaaaaggaa aagaaccgca
     5041 agagccaatt attgaggaag ccgggatcaa agaatacaaa aatgtctggg tatttgtaga
     5101 acatacagaa ggggagcctg ctacggtttc atgggaatta ctgggtgcag gtgcaaagtt
     5161 ggcaagctcc ctg
//
LOCUS       DATGBN010000127         1860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_320370, whole genome shotgun sequence.
ACCESSION   DATGBN010000127 DATGBN010000000
VERSION     DATGBN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320370"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VKL21_02855"
     CDS             <1..495
                     /locus_tag="VKL21_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMB44740.1"
                     /translation="DMTTLGKILGGGFPIGVIGGNREIMEKISPAGPVYQAGTFNGSP
                     VSMTAGLAVISALKKEKVHDRVNDAGDLLRSGLSDIIADLGQDCSLSGVGSMFKVFFG
                     DMPYNYQEALKCDTNKFNVFFKKMLNDGIFLPPSQFETNFLSVAHSQNDIDKTIETYQ
                     RNLK"
     gene            507..1427
                     /gene="hemC"
                     /locus_tag="VKL21_02860"
     CDS             507..1427
                     /gene="hemC"
                     /locus_tag="VKL21_02860"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HMB44741.1"
                     /translation="MRIGSRGSKLALAQANLVKDLLARKGVDTEINIIKTSGDTFTDR
                     PLHEVQGFGVFVREIDDSMLAGQIDIAVHSMKDIPTEHPPELTIAAVMKRDSPYDFLL
                     TRKDTKLKDLPEGSIIGTTSLRRRAQLSRFRTGFAIKELRGNIDTRLRKLKEGQYDGI
                     FLAEAGLERLKWDIPGERLDPDDFVPSANQGTVVIVTKKDSAAQTAVQELNDMQTRLE
                     TRIERIIIGILGGGCLVPIGAFARTEGNEIHVRTEVLSVDGKRCVKIDEFINPAEYEK
                     EAERIGNELNKKGGGKLVDEAVKMFAAKRG"
     gene            1430..>1860
                     /gene="cobA"
                     /locus_tag="VKL21_02865"
     CDS             1430..>1860
                     /gene="cobA"
                     /locus_tag="VKL21_02865"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HMB44742.1"
                     /translation="MKGKVYLVGSGPGDPELLTIKAKRLIESAQVILYDQLPGKAILD
                     MLPESAEKIDVGKYAGNHKLSQWQINELLVKRAKEGKIVVRLKGGDPYLFGRGGEEAQ
                     TLAGEGINVEIVPGITSAIAVPAYAGIPITHRDFASMVTFIT"
BASE COUNT          587 a          372 c          470 g          431 t
ORIGIN      
        1 gatatgacca cccttggaaa gatacttgga ggcggtttcc ctatcggagt gatcggggga
       61 aatcgagaga taatggagaa aatttccccg gcgggacctg tttaccaggc agggacattc
      121 aatggcagtc ccgtttccat gacagccgga cttgctgtga taagcgcgtt aaaaaaagag
      181 aaagtccacg acagagtaaa tgatgcaggt gatttattaa gatcaggatt atctgatatc
      241 atagcagatc ttgggcagga ttgtagcttg agcggagtgg gcagcatgtt caaggtgttc
      301 ttcggggata tgccctataa ttatcaggaa gctttgaaat gcgatactaa taaattcaat
      361 gtatttttca agaaaatgct aaatgacggc atctttttgc ccccctcaca gtttgagaca
      421 aacttcctgt ccgttgccca ttcacaaaat gacatagata aaaccataga aacttaccag
      481 aggaatttga aataattatg aaaaaattgc gtatcggttc aagagggagc aaacttgctc
      541 ttgcccaggc aaacctggtg aaagacctcc ttgcaagaaa aggagtagat acggaaatca
      601 atataatcaa aacatccggc gatacattca ccgacaggcc gttgcatgaa gtgcagggat
      661 tcggggtttt tgtgcgtgaa atagacgatt cgatgcttgc cggccagata gatatcgcgg
      721 tacacagcat gaaagatatc cctaccgaac atccacccga acttacaata gctgcagtaa
      781 tgaaacgcga ttcgccttat gatttcctgc ttacgcgcaa agatacaaaa ttgaaagacc
      841 ttcctgaagg ttcgatcatt ggaactacaa gcctgagaag gcgcgcccag ctatccagat
      901 tcaggacagg tttcgccata aaggagctaa gaggaaatat cgacactcga cttcgcaaac
      961 ttaaagaagg gcagtacgat ggtattttcc ttgcagaagc aggacttgag agattgaaat
     1021 gggacatacc gggagaacgt cttgatcctg acgattttgt tccatctgcc aaccagggaa
     1081 cagtagttat tgtgacaaaa aaggattcag cagcgcaaac agcagtgcag gaattaaatg
     1141 atatgcagac acgtcttgaa acgaggatcg aacggataat aatcgggatc cttggtggag
     1201 ggtgtcttgt tcctatcggc gcttttgcac ggactgaagg caatgagata catgtacgta
     1261 cggaagttct ttcagtggac gggaaacgtt gcgtaaaaat agatgaattt ataaaccccg
     1321 ccgaatatga aaaggaagcc gagcgaatcg gtaatgaatt gaacaaaaaa ggtggcggca
     1381 aacttgtgga tgaagcagta aaaatgtttg cagctaaaag aggctaaaaa tgaaagggaa
     1441 agtttatctt gtaggctcag gtcccggcga cccggagctt ctgactataa aagcaaaaag
     1501 gcttattgag agcgcccagg tcatccttta tgaccagctt ccgggtaaag cgatacttga
     1561 tatgctgccg gaatcagccg aaaaaataga tgttggtaaa tacgcaggga atcacaaact
     1621 ttcccagtgg cagataaatg agctccttgt caaaagggca aaagaaggaa agatcgtggt
     1681 acggttaaaa ggcggagacc catacctttt cggtcgcgga ggcgaggaag cacagacgct
     1741 tgcaggagaa ggtatcaatg tggagatcgt cccggggatc acctcggcaa ttgcagttcc
     1801 agcgtatgcg ggaattccaa ttacacatcg cgattttgca tccatggtaa ctttcatcac
//
LOCUS       DATGBN010000128         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_87679, whole genome shotgun sequence.
ACCESSION   DATGBN010000128 DATGBN010000000
VERSION     DATGBN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87679"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VKL21_02870"
     CDS             <1..707
                     /locus_tag="VKL21_02870"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMB44743.1"
                     /translation="KKYAIIGVPSQASVLNKKIITEITKDFFDGVMVASEPFCVAYNL
                     GELEHSMVVDIGAGTTDICRVHGTLPEPDDQISSNKAGDYIDAELVRLISEEYTNVRV
                     TNEMAKHWKELSSFVGGPEEEIVVDVPVDSSILKLSITKEIKTSCESIIPDIINGISK
                     LISTYDPDFQEKLRNNIWLAGGGSLIRNLDVQLEKELELIDGGKVFKDTDPIFSGANG
                     ALKLAQNMPPEFWQTT"
     gene            708..1319
                     /locus_tag="VKL21_02875"
     CDS             708..1319
                     /locus_tag="VKL21_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0097588 - archaeal or bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlaD/FlaE family flagellar protein"
                     /protein_id="HMB44744.1"
                     /translation="MLEELTGTSIETQRKWLKFLIERVGHNNLPKLLNYYMSIGWISE
                     SASIRLLEMAGSEKRYKGVSWTLSAEEQRISRFFIEKLKGVKIEDSLLNVPVPGKAKP
                     DIEKKIEIRRQEHIHPVEKKKMEISIHRREVTINNLEQKLEEKYAEIEELKGRIQELE
                     NAFEETRKELMKNKIYMDIMDQNIRLKKAVRFGKNIEKSKRSR"
     gene            complement(1328..1804)
                     /locus_tag="VKL21_02880"
     CDS             complement(1328..1804)
                     /locus_tag="VKL21_02880"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMB44745.1"
                     /translation="MRIPDEMRNILYSNYFGYVCTSDKNDQPHLTPVFFVYDENSQKL
                     FFITNETSKKVKNLCENPNVSITIDIRDPVNPFNNEGVMAQGRAIITDKAPIYFLSEE
                     TLQLYIDIYLEFKSKYQKVIDNKPLGENDVIIQINVEKMVYWKGPHFKSLKFKKEE"
     gene            complement(1808..>2844)
                     /locus_tag="VKL21_02885"
     CDS             complement(1808..>2844)
                     /locus_tag="VKL21_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMB44746.1"
                     /translation="LVAVSWSLLILQLAPTLRKFRAPLSKDENELLERYKGILFHKNH
                     YEAVPGVVIFIPFMFYLFNFGRDLRDMWLGKNPHVILLLFVSLLIWDICYRMGLGIWT
                     SVLALWRSLRLKKLAEKRTELEHTPYTELRYLQQLDINNVFFGLISLLLLPLFKTDDF
                     LVAVTLFFMVFVTLTSLISAYIISKVPWLPPDIYNLVNESSFAYIGTSLKGKTHVTPV
                     VYVFDGQKILFNTSKEARKLKIMQQNNKVAFLIDKRDMSNIYQNKAVLFTGEVKIYGI
                     MDIPFRFIHMLQALLLFMKKYPEYTKKYSTSELPKAWQLTPIIARILVEVKPVKIIYW
                     RGAKQISVPV"
BASE COUNT          910 a          529 c          594 g          811 t
ORIGIN      
        1 caaaaaagta tgcaattata ggtgttccat cccaggcaag tgtacttaat aaaaagatca
       61 ttactgaaat tacaaaagat ttctttgatg gtgtaatggt tgcaagcgaa ccattctgtg
      121 tagcatataa tctgggagaa ctggaacatt ccatggtagt tgatataggc gcaggaacca
      181 cggatatttg cagggtacat ggcactctac cggaacctga tgatcagata agcagcaaca
      241 aagccggcga ttatattgat gctgaattgg tgaggcttat atccgaggaa tatacaaatg
      301 taagggtgac aaacgagatg gctaaacatt ggaaagaact gagttccttc gttggcggac
      361 ctgaagaaga gatagttgtg gatgtacctg ttgattcttc catactcaaa ttatcaatca
      421 caaaagagat aaaaacatca tgtgagtcca ttatcccgga tataataaat ggtatctcca
      481 aactgatatc cacttatgat cctgacttcc aggaaaaatt acgaaataat atctggctgg
      541 caggcggagg aagcctgatc cggaatcttg atgtccaact ggaaaaggaa cttgaattaa
      601 tagatggcgg aaaggtcttt aaagatactg atccaatttt cagcggcgcc aatggggcct
      661 tgaaactggc ccagaatatg ccgcctgaat tctggcaaac cacatagatg ctggaagaac
      721 ttactggaac ttcgatagaa acccagcgaa agtggctgaa atttctcatt gagagagtcg
      781 gccacaacaa cctcccaaaa cttcttaatt attatatgag cataggctgg ataagtgaat
      841 ctgcatctat cagactgctt gagatggcag gttcggagaa acgctataag ggggtatcct
      901 ggactctctc tgctgaagag caaagaattt caaggttttt tattgagaag ctaaaaggag
      961 tgaaaatcga agattctctt ctaaatgtgc ctgttccggg aaaagcaaaa ccggatattg
     1021 aaaaaaagat cgagataagg cgacaggaac atattcatcc tgtggaaaaa aagaagatgg
     1081 agataagtat acaccgccgc gaggtcacta tcaataacct tgaacaaaaa ctggaagaaa
     1141 agtatgctga aatagaagaa cttaagggaa ggatacagga gcttgaaaat gcctttgaag
     1201 aaacccggaa ggaactgatg aaaaataaga tatatatgga tatcatggat cagaacataa
     1261 gattgaaaaa agcggttcgc ttcggcaaga acatcgaaaa atcaaaacga tcgcgctgat
     1321 ccgggatcta ctcttctttt ttgaatttta atgatttgaa atgagggcct ttccagtaca
     1381 ccattttctc aacatttatc tgtattatca catcattctc gccaaggggc ttattatcta
     1441 tgactttctg gtatttgctc ttaaattcca gatagatgtc aatatataat tgcagcgtct
     1501 cttcagagag gaaatatatt ggcgccttat cagtaataat tgccctgccc tgggccataa
     1561 ctccttcatt attgaagggg tttaccggat cccggatatc tatagttata ctgacattgg
     1621 gattttcaca caggttcttg accttttttg atgtctcatt tgtgataaag aagagtttct
     1681 gggaattctc atcatatacg aaaaaaaccg gagtcaagtg tggctggtca tttttatcgc
     1741 ttgtgcatac ataaccgaaa tagttgctat acaggatatt tcgcatttca tcgggtattc
     1801 gcattgttta caccggcaca cttatttgtt ttgcgcctct ccaatagatt attttaaccg
     1861 gttttacttc aacaagtatc cttgcaataa ttggtgtcaa ctgccaggct tttggcaatt
     1921 ctgatgttga atatttcttt gtatattcgg gatatttttt catgaagagc aaaagtgcct
     1981 gcaacatgtg aataaatcga aaaggaatat ccatgatacc gtaaattttg acttctccgg
     2041 taaacagaac tgctttgttt tgatatatat tgctcatgtc cctcttatct atcaggaaag
     2101 ccaccttgtt gttttgctgc atgatcttta attttcttgc ttcttttgaa gtattgaaca
     2161 ggattttctg cccgtcaaaa acataaacaa ctggcgttac atgggttttt cctttcaatg
     2221 atgtgccgat atatgcaaaa cttgactcgt tgacaagatt atagatgtcg ggcggaagcc
     2281 aggggacttt tgagataata taggctgaaa taagtgatgt cagggttaca aaaaccatga
     2341 agaacaatgt gactgcgacc aggaaatcat ctgtcttgaa taacggcaat aaaagtaggg
     2401 aaatcaaccc gaagaagaca ttattgatat caagctgttg aaggtatcga agttcagtat
     2461 atggcgtatg ttcaagctcc gttctttttt ccgcaagttt ttttaatcgc agggatctcc
     2521 atagcgcaag gactgatgtc catatcccaa gccccatcct gtagcaaata tcccatatca
     2581 ggagacttac gaataataat aaaatcacat gcggattttt tccaagccac atatcccgca
     2641 gatccctgcc aaaattgaaa agataaaaca taaaaggaat gaaaataaca acccctggaa
     2701 ctgcctcata atgattttta tggaaaagta ttcccttata ccgttcgagc aattcatttt
     2761 catccttact taacggggct ctgaattttc gcagtgttgg cgcaagttgc aggatgagaa
     2821 gactccatga tactgcaacc agga
//
LOCUS       DATGBN010000129         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_242919, whole genome shotgun sequence.
ACCESSION   DATGBN010000129 DATGBN010000000
VERSION     DATGBN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242919"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VKL21_02890"
     CDS             <1..356
                     /locus_tag="VKL21_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HMB44747.1"
                     /translation="HIQTLAQASQKDHEEMIALYNEIDTHRKEADSFHAQLNEKFKGI
                     APLRKKISAIKAQIPNLRDELGVYLEQIKEYQLGRDEQKNVGKREHAREKFKKDGRLS
                     LDEFRLLVESEDIKL"
     gene            complement(346..1455)
                     /locus_tag="VKL21_02895"
     CDS             complement(346..1455)
                     /locus_tag="VKL21_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HMB44748.1"
                     /translation="MKYQSHFRFFKKNMQSIAVLVPLVPGSDIIEQPCDGIMIYSFAS
                     PQAPFIFREVSRARTGSVFIAGGPHPSVRPEETLRFFDYVVIGEGEETLPELIDVILN
                     KGDISSVRGIAYKNEAENIVFTEKRNEIDLDKYPPFDSRIMHNTIEITRGCPFSCAYC
                     GTWKLFGHNMRHRSIDDISKYAKFLRDIRFTSPNAFAYGSDGIHPRIEKIESLLRALS
                     PGRIFFGTFPSEVRPEFINDRILELISDFCENTTINMGGQSGSQRILESIMRKHTVED
                     IIIGAEKCIDHGFTPVVDFIFGLPNETEYDQLETLKLIKLLTGKGGNVHSHYFMPLPG
                     TTFENDVPAPVSKQVGKVMGELALLGKTTGVWSKA"
     gene            complement(1452..>2021)
                     /locus_tag="VKL21_02900"
     CDS             complement(1452..>2021)
                     /locus_tag="VKL21_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HMB44749.1"
                     /translation="CIWESNIHSTYCFNGPLIVLRKKAFSPLPEKYGASDAGMALNII
                     RNGYRCIYVSSAKFSEYIAKQLEQQRRQKLRRSSRLIEATLLNIDMISFKYGRFGTFI
                     FPLRILMFIFVPALFFISIILWSYIMGEINILYGLMVWMVLFFALFTGMWSSNIISSF
                     IWHQIYLLLSLVYLFRGMHMWKSVDREKV"
BASE COUNT          608 a          436 c          417 g          560 t
ORIGIN      
        1 cgcatatcca gacgcttgcc caggcatccc agaaagatca tgaagagatg atagcccttt
       61 ataatgaaat cgacacgcat cgcaaggaag cagactcctt tcatgcccaa ttgaatgaaa
      121 aattcaaagg aattgcgccg cttcgaaaga aaataagcgc tataaaggca caaatcccca
      181 atctaagaga tgaactaggg gtttatcttg agcagatcaa agaataccag cttggccgcg
      241 acgagcagaa aaatgttgga aaacgcgagc atgccaggga gaagttcaag aaagacggac
      301 gtttgagcct ggatgagttc aggttgcttg ttgaaagcga ggatatcaag ctttagacca
      361 cacgcctgta gtttttccaa gaagtgcaag ctcgcccatt acctttccca cctgttttga
      421 taccggggca ggcacatcat tttcaaatgt tgtgccggga agcggcatga aataatggga
      481 atgcacgttt cctccttttc cggtaagcaa ttttattaat ttgagagttt ctaactggtc
      541 atattcggtc tcatttggaa gcccgaatat gaaatcaacc acaggagtaa acccatggtc
      601 aatgcatttc tcagccccga tgataatatc ttccacggtg tgttttcgca ttatgctttc
      661 aagtatcctc tggcttcctg attgtcctcc catgtttatc gtggtatttt cgcaaaaatc
      721 cgaaataagc tcaagtatcc tgtcgtttat gaattccggc ctcacctccg aaggaaacgt
      781 tccaaaaaat atccttccag gtgataatgc acgcagaagc gactcgattt tttcaatgcg
      841 aggatgtatt ccatccgaac cataagcaaa agcgttggga gatgtaaacc tgatatcccg
      901 gaggaacttt gcatattttg agatatcatc aatgctcctg tgtctcatat tatggccgaa
      961 taatttccag gttccgcagt aagcgcatga aaaaggacag ccgcgtgtga tctctatagt
     1021 attgtgcatg atccgggagt caaatggcgg atatttatct agatcgatct catttctttt
     1081 ttccgtaaaa acgatatttt ccgcctcatt cttataggca atacccctga ccgatgaaat
     1141 atctccttta tttagaatta catcgatcaa ttcaggcaat gtttcctcgc cttcgccgat
     1201 aaccacatag tcaaaaaatc gcaaagtttc ttcaggccgg acagaaggat gagggcctcc
     1261 ggctatgaat acggatcctg tccgcgcgcg ggacacttcc cggaaaatga agggcgcctg
     1321 gggtgatgca aagctataga tcattatgcc gtcacagggc tgttctatta tatccgatcc
     1381 aggaacaaga ggcacaagga ccgctatact ttgcatgttc tttttgaaga acctgaaatg
     1441 actttgatac ttcaaacttt ctccctgtca acagatttcc acatatgcat acccctgaat
     1501 aaatatacca ggcttaatag tagatatatc tggtgccata tgaaggagga gataatattt
     1561 gaactccaca tacctgtaaa aagagcgaaa aacaatacca tccagaccat taatccatat
     1621 aatatattaa tttctcccat gatatatgac cataatatta tgctgataaa aaataacgct
     1681 ggaacaaata tgaacatcag tattctcaat ggaaatatga atgttccaaa tctcccatat
     1741 ttaaaagaga tcatgtcgat attaagaagt gttgcttcaa ttaatcttga tgacctgcga
     1801 agtttctggc ggcgctgttg ttcaagttgt tttgcgatgt attcactgaa ttttgcggaa
     1861 gaaacataaa tgcacctgta accattgcgg ataatattta gcgccattcc tgcatcagat
     1921 gcgccgtatt tttccgggag tggtgaaaaa gcttttttcc gcaggacaat gagcggacca
     1981 ttaaaacaat atgtggagtg aatgttgctt tcccagatgc a
//
LOCUS       DATGBN010000130         4828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_87720, whole genome shotgun sequence.
ACCESSION   DATGBN010000130 DATGBN010000000
VERSION     DATGBN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87720"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VKL21_02905"
     CDS             <1..689
                     /locus_tag="VKL21_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMB44750.1"
                     /translation="PLYSGNVGSVARVMKNFGFSELVLLSPCEIDMPARVMSVHAYDI
                     IENARIEFSLNDALKGSNIIIGMTGLPGKTDNKHFRIPAYSPRQLKEKLAGNNGVISL
                     VFGREDAGLRNEELELCDIIVNIPTSQVYQSMNLSHAVAVVLYELSDIKAGERYIADH
                     FDIELLYEHIDEVLDDIEYKEHKEDKTKLMLSRILGRAELTAREVQTLRGILRRIQWK
                     LRTGEYGKSD"
     gene            673..2178
                     /locus_tag="VKL21_02910"
     CDS             673..2178
                     /locus_tag="VKL21_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HMB44751.1"
                     /translation="MVNPIKFILGCIFGKKPRIAQSQMIKPSELKGDIKLGEFIPFFI
                     VASIEVGNTTTKCILTATNMKDGRTRVLNKTVKMTRDVRKPKPDERIFGSTLNGVSLT
                     RESIAQLVKDTLIESHEAVNLDIATDLDFAVRSTGVVAGFSSPHEVGEFIKALADGCL
                     LAGIPPKKMVPAMSIDNIPVKFKNNSLIDKIIFTGAVGGVLPPVGSTGVEIVANEMEG
                     ELATAGIKEGAKWSGVDFRNPVFSMDFGTTLKGRITNDEIPYARTIANLCGFAGAIPD
                     AVIKGTGLVDKRYGAALDLFSDNKERMIWLSRGNVIRKYCDEILSHVKIEIVPGNVNR
                     YGSVPVDPVAARNIGVILIGCDVGENGSLLHELTDIGSELYSKHGLRTMFATLDLVSA
                     MMVGRIIKLAIEHKLVSGKTSIGLTGRAAITGCKPYLILKSIEKLGIFKEPREHVVFV
                     DDGLARGAAVMARCMNSMGVPKNPIGGIRGGGCVLKQRIDYYEKSKERNVS"
     gene            2175..2627
                     /locus_tag="VKL21_02915"
     CDS             2175..2627
                     /locus_tag="VKL21_02915"
                     /inference="COORDINATES: protein motif:HMM:NF020570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HMB44752.1"
                     /translation="MKALLMMGCPELPVQTAAALYIANKLNREGFEVISAGNKAAISL
                     LLNSDPEKHYIKQVMDLDRCIGALAEKKMDFDLCFVFIHSDSGISYLATIKSISKAKT
                     IAVIFGKEIEPLIEASTDSIIIAAKAVHNPTPLRAQIDGVKSWAALTR"
     gene            2606..2881
                     /locus_tag="VKL21_02920"
     CDS             2606..2881
                     /locus_tag="VKL21_02920"
                     /inference="COORDINATES: protein motif:HMM:NF020549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HMB44753.1"
                     /translation="MGCVDEMNYEILLPNSSFKECAEYIKKNFKEIYYVPAGYKIFDN
                     YLVGIPPIPIAVDNDDIIMPYVKPCHGCFVLRVPGIEEIGVLRAGKS"
     gene            3400..4515
                     /locus_tag="VKL21_02925"
     CDS             3400..4515
                     /locus_tag="VKL21_02925"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB44754.1"
                     /translation="MIKAYMFRIYPNKNQDVILNRTLSTCRHLYNNSLAERKKQAELN
                     RLKKSFDVFPWGYPEWVNKYGQMRYLTATKTEYQKDVYGQVLQDAIKRSDKSMTNFFK
                     GFGYPRFKGRNRYNSFTYPQNGFSIKDGKLHLSKIGNIKIILHREIEGKIKTCTIKKD
                     IDHWYVSFSCEIEEPKPVKIKTKTGIDVGLIDLITLSNGQQVKPPKFLRESEDKLTQE
                     QKQLSRKKKGSGKRNRQKIIVAKVHRKIRNQRKDFAHKLSRNLADTYDSIKFEDLQIK
                     NMVQNHHLAKSINDAGWYQLMQFTKYKAEYAGKLVEFVNPAGTSQTCICGFSVPKDLS
                     VRTHICPSCGLVMGRDQVSAILIENKSISKYGRNYRN"
     gene            complement(4643..>4828)
                     /locus_tag="VKL21_02930"
     CDS             complement(4643..>4828)
                     /locus_tag="VKL21_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44755.1"
                     /translation="FPFVHGMSHHTKATYSSLAFQAVVTDIRELCTILRSTALPVFFT
                     QEQTCAVGFKPRWIQCR"
BASE COUNT         1530 a          906 c         1113 g         1279 t
ORIGIN      
        1 aaccgcttta cagcggaaac gtaggttctg tagcccgcgt catgaaaaat tttgggttca
       61 gtgaacttgt acttctaagt ccctgcgaaa tcgacatgcc tgcacgcgta atgtctgttc
      121 atgcctatga tattatcgag aatgcaagga tcgaattctc tttaaatgac gctttgaaag
      181 gctcaaatat tattatcggg atgacgggac ttccggggaa aaccgataat aaacacttca
      241 ggatccctgc ttatagcccc aggcaattga aagaaaaact ggccgggaat aatggagtaa
      301 tatcccttgt tttcggaagg gaagatgcag ggcttcgaaa tgaggaactt gaattgtgcg
      361 atataatagt taatatcccc acaagtcagg tttaccagag catgaatctt tctcatgctg
      421 ttgctgttgt tttgtatgaa ctaagcgata taaaagccgg ggaaaggtat attgctgacc
      481 attttgatat cgaactttta tatgagcaca ttgatgaagt actcgatgat attgagtata
      541 aggagcataa agaagacaag acaaagctta tgttaagtag aatattaggg cgggctgaat
      601 tgactgcacg agaggtgcag acccttcgtg ggatactgcg ccgcatccag tggaaattaa
      661 gaacaggtga atatggtaaa tccgattaaa ttcattcttg gctgcatttt tggcaaaaag
      721 ccaagaattg cgcaaagtca gatgatcaaa ccttccgaac tgaaaggtga cataaaattg
      781 ggcgagttta ttcccttctt tatagttgca tccatagaag tcgggaatac tacaacaaaa
      841 tgcatcctta ctgccacaaa tatgaaagac ggacgcacgc gcgttttgaa caagaccgtt
      901 aaaatgacgc gagacgtcag gaaaccaaaa cctgacgaaa ggatctttgg ttctaccctt
      961 aatggtgtat cattaactag agaatccatt gctcaactag taaaggatac tctgattgaa
     1021 tcgcatgagg ctgtaaacct tgatatcgcc acagaccttg atttcgctgt gaggtcaact
     1081 ggtgtagttg ctggtttttc atcgcctcat gaagtcgggg aatttataaa agcgcttgcg
     1141 gatggatgcc tccttgcagg aatcccgcca aaaaagatgg ttccggcaat gtccatagat
     1201 aacatcccgg ttaaattcaa aaacaattcg ctgatcgata agatcatatt tacaggggct
     1261 gtcgggggtg ttcttcctcc tgttggttca acaggggttg agatcgttgc aaatgaaatg
     1321 gaaggcgaac tcgcaacagc cggcatcaaa gaaggtgcca aatggtcagg ggtggatttc
     1381 aggaatcctg ttttttcaat ggatttcggg acgaccctga aaggccgtat taccaatgat
     1441 gaaatccctt atgccaggac tattgcaaat ctgtgcggtt ttgcaggcgc gattcctgat
     1501 gcagtaataa aaggtacagg tcttgtagat aagagatacg gggctgccct tgatcttttc
     1561 agcgacaata aagaacgcat gatatggctt tccaggggca atgttatccg gaaatattgc
     1621 gatgagatac tttcacatgt aaaaatcgag atagtacctg gtaatgtaaa tcgatacgga
     1681 agcgtcccgg tcgaccctgt cgctgcaaga aatataggtg taatacttat tgggtgcgat
     1741 gttggagaga acggaagtct gctgcatgaa ttaacggata ttggctcgga attatactca
     1801 aaacatggtt taagaacaat gtttgccact cttgacctcg tatctgcaat gatggttggg
     1861 agaatcataa aactggccat cgaacacaag cttgtttccg ggaagacatc catcggattg
     1921 acaggaagag ctgccattac aggctgcaaa ccttacctga tattgaaaag tattgaaaag
     1981 cttgggattt tcaaagaacc aagagagcat gttgtgttcg tggatgatgg tcttgcccgc
     2041 ggcgccgccg ttatggcgcg ctgcatgaac tccatgggag tacccaaaaa tcccatcggg
     2101 ggtataaggg gcggcggctg cgtcctgaaa cagcgtatag attattatga aaaatcaaaa
     2161 gagaggaacg tatcttgaaa gcacttttaa tgatgggatg ccctgaattg ccagtccaga
     2221 cagcagcggc attgtatatc gctaataaat tgaaccggga aggatttgaa gttatatccg
     2281 cagggaataa agctgccata agtcttcttc ttaattccga tcctgagaaa cattatataa
     2341 agcaagtgat ggacctcgac aggtgtatcg gcgctctcgc tgaaaagaaa atggattttg
     2401 acctgtgttt tgtttttatc catagcgata gcggcatttc atacctggcg acaataaaat
     2461 cgatctcaaa agcgaagacg atagcggtta ttttcggaaa agagatcgaa cctctaattg
     2521 aagcaagtac ggattctatt ataattgccg caaaagctgt tcataacccc acgccgcttc
     2581 gggcacagat agacggggtg aagtcatggg ctgcgttgac gagatgaatt atgagatatt
     2641 gctgccaaat agctctttca aggaatgcgc tgagtatata aagaaaaatt ttaaagaaat
     2701 atattatgtt cctgcgggtt acaagatatt tgataattat cttgtgggga tcccgccaat
     2761 acctatagct gttgataatg atgatattat catgccttat gtaaagccat gccatgggtg
     2821 ttttgtgctg agggtacctg gaatagagga aattggggta ttgagggcgg ggaaatcata
     2881 gtcgtgcgga attatatggt atattataaa taaacaacga actggtacga actgatacga
     2941 actcatgtcg aaaggtactg taagacgtaa ttttttatga aaatattccg gagttcgttt
     3001 cagttcgtta ggagttcgtt gttttttttc ttcagtatcc ttgacgctaa aagaaaaata
     3061 ctatccgctt tttggcagga atggcgttag ctaggtaagg aaggagttca tacttcttgt
     3121 ctgtaaataa agggtaccgg ttccccgaaa ctctgctaca agcccttcgc cgcttaatat
     3181 ggttgatttg agaccgccga ctctttttac tttacgcggg gtcaactaat catgaaccga
     3241 ttggttctta gatactgtat gaaaaatttc gttttttcat acgaaggggt agagatcgcc
     3301 caatgccttt aggcttgggc tgaatcggcg tgccttgaat aaatactttc accccactct
     3361 gataaacaat atatattctt aaaataatgt caatacttga tgataaaagc ctatatgttc
     3421 agaatatacc cgaacaagaa tcaggatgtt attcttaaca ggacactctc tacatgcagg
     3481 catctctata acaattctct tgcagaacga aagaagcaag cagagcttaa tcgactcaaa
     3541 aaatcatttg atgtatttcc gtggggatat cctgaatggg tcaataaata cggtcaaatg
     3601 cgttatttaa cagctacaaa gacagaatat caaaaagacg tatatggtca ggtacttcaa
     3661 gatgcaataa aacgatctga taaaagtatg actaactttt tcaaaggttt tgggtatccc
     3721 cggttcaaag gacgaaacag atataatagt ttcacttatc cgcaaaatgg tttttcaatc
     3781 aaagatggaa aactacatct ttcaaaaata ggaaacatca agattatttt gcatcgagaa
     3841 atcgaaggca agatcaagac ctgtaccatc aaaaaagata tagatcattg gtatgtttcc
     3901 ttttcctgtg aaatcgaaga acctaaaccg gttaaaatta agaccaaaac cggaatcgat
     3961 gtgggtttaa ttgatttgat aaccctgagc aacggacaac aggttaaacc gccaaagttc
     4021 ctgcgggaat cagaggataa attaacacag gaacagaaac agttaagccg aaaaaagaaa
     4081 gggtcaggaa aaaggaacag gcaaaagatt atagtggcga aagtacacag gaaaatcagg
     4141 aatcagcgaa aagatttcgc tcataaacta tcaagaaatc tggccgatac ctatgacagc
     4201 atcaaatttg aagacttgca gataaaaaac atggtgcaga accatcatct tgctaaatct
     4261 attaatgatg ctggctggta tcagttaatg caattcacga aatacaaagc agaatacgct
     4321 ggtaaactcg tggaattcgt taatccagca ggaacatctc agacctgtat ttgtggattc
     4381 tctgttccga aagacctgtc agtgaggaca catatttgtc cttcctgtgg tcttgtaatg
     4441 ggaagagatc aagtgtctgc gatattaatc gaaaataaat caatcagtaa gtacggtagg
     4501 aactaccgga attaacgccc gtcaaggata tctcagtaga gtatcaatgc agcgggacgc
     4561 tctgcggctt tagcccggag cagttcacgc gtgcctcgaa gcacgagaaa gaaagaacag
     4621 caaaaaggat accaaaatga atttaacggc actgtatcca ccggggttta aatcccactg
     4681 cgcaggtctg ctcctgcgta aagaaaacgg gaagtgcagt actgcgaagt attgtgcaga
     4741 gttccctaat atcggtaacc acagcttgaa acgcaagtga actgtaagtg gccttagtat
     4801 gatgcgacat tccgtgaaca aatggaaa
//
LOCUS       DATGBN010000131         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_9753, whole genome shotgun sequence.
ACCESSION   DATGBN010000131 DATGBN010000000
VERSION     DATGBN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9753"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /gene="mcrC"
                     /locus_tag="VKL21_02935"
     CDS             complement(<1..584)
                     /gene="mcrC"
                     /locus_tag="VKL21_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HMB44756.1"
                     /translation="MFGRETQLVDCREAMGLGRGGGLAQRGTLSEAARPDVVAIAMTP
                     GRRHITKPVCEITQGLRRESIQVSVLVLEAGAGIPMDDVGTSTISKGYGANFGLTAKE
                     IEQIARHKVVLINMGNVNSHILSKTKKILKYINIPAVIACEYPIDFEDFAKVGIKTKF
                     VRPKKSETEGTVVAIVSGITRGETCSRIKLNELA"
     gene            complement(587..1006)
                     /locus_tag="VKL21_02940"
     CDS             complement(587..1006)
                     /locus_tag="VKL21_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HMB44757.1"
                     /translation="MSQKIIDALNKDREGELSAIIQYMKHHYEGEGMESPAILDIFKS
                     TAKDEMEHAEKLGERINYLGGIPTKKPEPIVEGGDIKKMVQDDLAKENAAIKQYKEHI
                     KLAIEENDPTTRLMLEEILSDEEGHADTWETTLGIKK"
     gene            complement(1069..1464)
                     /locus_tag="VKL21_02945"
     CDS             complement(1069..1464)
                     /locus_tag="VKL21_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMB44758.1"
                     /translation="MEPKKVIDSIEGKMGFTPEIMNMMGEMNPELFDHYKKCDDGIQE
                     DGALSAKVKVLMSLAVMASQRCEPCCESQMRSALNHGATKEEIMETMNVIFITSGAPG
                     VAVCRRALNLMEGKEEGHGHGCMGGRRRH"
     gene            complement(1503..1883)
                     /locus_tag="VKL21_02950"
     CDS             complement(1503..1883)
                     /locus_tag="VKL21_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMB44759.1"
                     /translation="MQTIEIMEKTFDSESNDIALYLAMSKKAEDEGQTEIATYLINVA
                     MDEAAHAAQFATLLGKVKDTKTNLVNMLDGEIQAEKDKAHAAKVAFAEGNHEASRFFE
                     KSMHDETRHKEGIQKILSKLQEKD"
     gene            complement(1892..2293)
                     /locus_tag="VKL21_02955"
     CDS             complement(1892..2293)
                     /locus_tag="VKL21_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HMB44760.1"
                     /translation="MEVIIDYTRETKLNVKSAVEKLTEDLKTNKWGVLSVIDVKKIFS
                     EKLNIEYDDYIILDVCNPGFAYQGLLINKQAGLVLPCKIVVYSENGKTRISLYKPTVA
                     LSTAGSLPGYDELSKLAIEAEAGLKKVIDLQ"
     gene            complement(2384..2905)
                     /locus_tag="VKL21_02960"
     CDS             complement(2384..2905)
                     /locus_tag="VKL21_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44761.1"
                     /translation="MEIENFKQRKDFNGRLETAKSLPDIFELVKAAVWECQRKSRGGL
                     MLGLANLGNHPQGFFGAFYPVGSNVIVMNKIPLERIKETRLELYKPYAFHVLLHEYLH
                     TLGYMSESGVRQMAHEITREVFGEEHLATRIAANTAHFFHNLVYPDATWQPDEMKMEL
                     VDGFDRGSAGYIG"
     gene            3041..>3561
                     /locus_tag="VKL21_02965"
     CDS             3041..>3561
                     /locus_tag="VKL21_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HMB44762.1"
                     /translation="MVKLTDKNIKWIIRHTEIQKDETTESISLMYKISERRVQQLRKE
                     YKDSGEIPRLIKSRRPKTELSDDDKEIIKKAWDKKRVGARLLFYNLESDGHKIPHNKI
                     NNYLLANNLTIPDPKKQKQRKRCRYERKHSGSLIHGDWHRSSESHPHVIVWLDDASRF
                     ALAGDEFKEATSE"
BASE COUNT          916 a          825 c          649 g         1171 t
ORIGIN      
        1 tttgccagtt cattcaattt gatccttgag caggtctcac ccctggttat tccgctgaca
       61 atcgccacca cagtgccttc tgtttcagat tttttgggtc ttacgaattt tgttttgatc
      121 ccgacttttg cgaaatcctc aaaatcgatg ggatattcac aggctattac tgcagggata
      181 tttatatatt tcaggatttt cttggtcttt gacagtatat gggaatttac attccccata
      241 ttgattagca caaccttgtg ccgtgcaatc tgttcgattt cttttgcagt caaaccgaaa
      301 tttgccccat atccttttga tatggtggaa gttccgacat catccatagg aatgccggcg
      361 cctgcctcaa gtacaagaac gcttacctgg atgctttccc ggcggagtcc ctgtgttatc
      421 tcgcatacgg gttttgtgat atgcctgcgg cccggggtca tggcgatagc cacaacatca
      481 ggtcttgcgg cctcagaaag ggttccgcgc tgggcaagac ccccgccgcg ccccagaccc
      541 attgcttccc tgcaatccac taactgggtt tctcttccga acatgattat ttctttatcc
      601 caagcgtggt ttcccaggta tcggcatgac cttcctcatc cgaaaggatc tcctcaagca
      661 tgagccttgt tgtggggtca ttctcctcga tggcaagttt gatatgttct ttatattgtt
      721 tgatggctgc attctccttt gccaggtcat cctgtaccat tttctttatg tctccgccct
      781 caacgatagg ctctggtttc tttgtgggga tcccgccaag ataattgatc ctctcaccaa
      841 gcttttcggc atgctccatc tcatcctttg cagtagattt gaatatatca aggatagccg
      901 ggctttccat tccctctccc tcataatgat gtttcatata ttgtattatt gcactcaatt
      961 caccttctct gtccttgttc aatgcatcga ttattttctg gctcatttta tcctcctatt
     1021 taactggata tcattgatag atcaatgaat ataatatttt tcaatcgctt aatgtcttct
     1081 tcttccgccc atgcagccat gaccatggcc ttcttctttg ccttccataa gattcagggc
     1141 tcttctgcat actgccaccc ctggcgcgcc tgaagtaatg aaaatcacat tcattgtctc
     1201 cattatttct tcttttgtgg caccatgatt gagtgcactc ctcatctgtg attcgcagca
     1261 tggttcacat ctctgggagg ccatgacggc aagtgacatc aatactttta cctttgcgga
     1321 aagagcgcca tcctcctgaa taccatcatc acatttctta taatgatcaa aaagttcagg
     1381 attcatttca cccatcatgt tcatgatctc gggagtaaat cccatttttc cttctatgct
     1441 atcgattact ttctttggtt ccatattttt tctcccatag tatatcttgg tttgaagttt
     1501 atttaatcct tttcctgcaa ttttgaaaga attttttgta ttccttcctt atgccttgtt
     1561 tcatcatgca ttgatttctc aaaaaacctg gaagcttcat gatttccttc ggcaaatgcc
     1621 acttttgctg catgagcttt gtccttttct gcctgtatct ctccgtcaag catatttaca
     1681 agattcgtct ttgtgtcctt aacctttccc aggagagtgg caaattgggc ggcatgcgcc
     1741 gcttcatcca ttgccacatt aatgaggtaa gttgcgatct cggtttgccc ttcatcttct
     1801 gcttttttag acatagcgag ataaagggca atatcatttg attccgaatc aaaggttttt
     1861 tccattattt caattgtttg cacattatca cctattgaag gtcaatgact ttttttaaac
     1921 ccgcttcagc ctcaatagca agtttactta attcatcata tcctggtagt gaacctgcag
     1981 tgctcaatgc aacagtcggc ttatatagtg aaatcctggt ttttccgttc tcagaataaa
     2041 ccactatctt acatggaagg accaatcccg cctgtttgtt tattaaaaga ccctgatatg
     2101 caaaccctgg attgcataca tcaagtatta tgtaatcatc atattcaata ttcaatttct
     2161 ctgaaaagat ctttttcaca tcgatgacac tcaatactcc ccacttgttt gttttgaggt
     2221 cctccgtaag tttttcaacc gcggatttta catttaattt tgtttctctg gtataatcta
     2281 taataacttc cacctcctta ctattgattt gtctttcaag tataataaaa ggaatagttg
     2341 tgcatcaaac gatcaaatat atataaatct gggttttcca tatttagcct atatagcctg
     2401 cgcttcccct gtcaaaacca tccacaagct ccattttcat ctcatcgggc tgccatgttg
     2461 catcaggata cacgagatta tggaaaaagt gtgccgtatt cgcagctatc ctggtcgcta
     2521 gatgctcttc cccgaatact tcccttgtga tctcatgcgc catctgcctg acgccgcttt
     2581 cactcatata gcccagtgtg tgcaggtatt catggagaag gacatggaat gcataaggtt
     2641 tgtaaagctc cagtcttgtc tcttttattc tttcaagagg gatcttattc atcacaatga
     2701 catttgagcc aacaggatag aatgcgccga aaaatccctg cggatgattc ccaagattgg
     2761 caagccccag catcaggcct ccacggcttt ttctctggca ttcccagaca gcagccttca
     2821 ccaattcaaa aatgtcaggc aggctctttg cagtctcaag gcggccgttg aaatctttcc
     2881 tttgtttgaa attttctatt tccattgatt gtttttcctc tgtgatatta ttcgctatat
     2941 tacgaacaat agtagattga agatatttaa ttaaatagtt ttcggtctcc gtaatggatg
     3001 tagagtacgg atattatttc gttcggcttc ttcggagcca atggtcaagt tgacagacaa
     3061 aaacataaaa tggataattc gtcatacaga gattcaaaaa gatgaaacta cagagagtat
     3121 ttcattaatg tataagattt cagagcgtag agttcagcaa ttaagaaagg aatacaaaga
     3181 ttccggagaa attccaaggt tgatcaaatc tcgtcgaccc aaaacagagc tttctgatga
     3241 tgataaagag ataataaaga aagcatggga taaaaaaaga gttggtgcca gactgctctt
     3301 ctataacttg gaatcagatg gacataaaat tccacataac aagattaaca attatctcct
     3361 ggccaataat ttaaccattc ctgatcccaa aaaacaaaaa cagcgaaagc gttgcagata
     3421 tgaaaggaag cattcaggaa gcctcattca tggagactgg catagatctt ccgaatccca
     3481 tccccatgta attgtttggc tggatgatgc atctagattt gcacttgcag gagacgaatt
     3541 caaagaagcg acaagtgaac a
//
LOCUS       DATGBN010000132        12064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_476098, whole genome shotgun sequence.
ACCESSION   DATGBN010000132 DATGBN010000000
VERSION     DATGBN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476098"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1882
                     /locus_tag="VKL21_02970"
     CDS             173..1882
                     /locus_tag="VKL21_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HMB44763.1"
                     /translation="MLKLLLLLFLLAGLSILNASAAPDQVVINSANWQDVYLGAHYAG
                     FNNIKSNFLVSSEHAYVLLQTLDTSKENILLVESDKAPFAFRYKTGLEGAGFIVDEII
                     SSGTALNIELAKRANTSDFIIVDDSYGYNAISVAPYAAQEKFFVLFANKENIDELYSF
                     LQANGVNRIIIYGFSDIEVKSKLAEFNPVIINNGSRFDNNIELLKRYKRVQQVFLTNG
                     EFIEEGIMSGKEPVLFPGRERVPNQVVEYIKSSGIKTGVVIGNELTGVAHQIKQATDM
                     NIFLKFGQGFASGGSSEVKALDMFPLPKYELAVDINSVKYNRATKELEVIYENKGNMG
                     VYLSSSIEVLSSGVRIATLGDKGSVYMDIGSTLAVAYASELNGQNLTAKFTTMFGEDP
                     GSFERALYKEMKIEMIEVIDSSSLNISKVSYDKAAHKIKVGIRNTGEVKTYAKTQIKF
                     VIYGDERIMVQDKAMLLEPGASKDVVFITTLSDTNIEENPQVNVRINYGERETVLIKV
                     LEGNYPLIITGDYTILIIVVILLIIGNTIRIQYRKKRIKDQKKSMEDSAAETEATEIS
                     KDT"
     gene            2143..2772
                     /locus_tag="VKL21_02975"
     CDS             2143..2772
                     /locus_tag="VKL21_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44764.1"
                     /translation="MIKSPTIKIINNLFIKTLLMIAFFVSIATGQAAVQANEGYIERI
                     DFAGNSYTEHWLGFYGIINNNTMMIGNSNVKNDTAIFTGIWAHEGDYLLITTSPSPPF
                     GLRAGNIASVDEITGWGDDSGSNTFSYSSNYRIPYSEAILTDVPSIYMFDNKKQYSRE
                     ALFSDSNGNPVFAVPIENIHNTSSHYFQLMLPDNGNLTYYFFYLPSDTQ"
     gene            2853..4058
                     /gene="thrC"
                     /locus_tag="VKL21_02980"
     CDS             2853..4058
                     /gene="thrC"
                     /locus_tag="VKL21_02980"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMB44765.1"
                     /translation="MYHLECIECHKKYPETEVIYTCTCGGLLDVIYDYSNIHLTKNDL
                     KGPLSVWKYRELLPVSREPISLQEGGTPLYRVDRIAKNIGLKDVYVKHEGMNPTGSFK
                     DRGMTVGVTKALELGMKTVACASTGNTSASLAVYGARAGVPAVVLLPSGKVALGKLAQ
                     ALMHGAKVLSIRGNFDDALKLVRDLCDREGFYLLNSVNPYRLEGQKTIAFEIADQLGW
                     KAPDRIVLPVGNAGNITAIYKGFKELKNLGLIDSVPKMTGIQAQGASPVVTAIKNNAR
                     AITPESHPETVATAIRIGNPVNSIKALIAIRESGGTAETITDEEIIRAQHELAYMEGI
                     GVEPASASSIAGLRKLVEQGVIGSDEIIACVTTGHLLKDSEHVLAVCPKPMEIDATIE
                     AVRKAVFSK"
     gene            4090..5205
                     /locus_tag="VKL21_02985"
     CDS             4090..5205
                     /locus_tag="VKL21_02985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMB44766.1"
                     /translation="MTLSSLIIKEIKRFGPISFRDFMEMALYYPGLGYYTSHKKKIGK
                     TGDYYTSSNLTPVFGEMLGKQIQEMWHILGKKEMTVVEMGAGTGLLSGDVLTYLANSP
                     ELFHDLNYCIVEKSPYLLKEQQDRLTHEKVNWYNSISELKGMRGCIFSNELPDAFPVH
                     VVEMENELMEVFVGYENGFIEILKPASDQLRDYLKELEVTLPFGYRTEINLDAIKWIR
                     EMGSALEKGFVISIDYGYSSRELYQDYRNRGTLLCYYNHTVNESPFEHIGEQDITSHV
                     NFSALDHWGQKKGLYLCGFTDQAHFLMGLRIDEYLKNLQEKSPVDYFKKMLPIKSLIM
                     DMGGTFKVMIQKKGVEFAELSGLKFPSEAPNRSRDAG"
     gene            complement(5319..5567)
                     /locus_tag="VKL21_02990"
     CDS             complement(5319..5567)
                     /locus_tag="VKL21_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44767.1"
                     /translation="MANKTASKSTTSSSWWNERGKIMAMKSETKTAKPKKKVKTKKAS
                     KAETGQTYICEICGCEMVCASDSSGEVVCCAEPMCIVV"
     gene            5670..6305
                     /gene="nth"
                     /locus_tag="VKL21_02995"
     CDS             5670..6305
                     /gene="nth"
                     /locus_tag="VKL21_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMB44768.1"
                     /translation="MVDDKVKKIISLLKNEYPGIKTALSYSDPLELLIATILSAQCTD
                     KQVNEVTKNLFKKYRTLQDYIKTPQEELEKDIYSTGFYRNKAKNIKGLSEILVNDFDS
                     KVPDTMENLLKLPGVARKTANIVLSGAFGKIEGIAVDTHVKRLSGRLGLTANTNPEKI
                     EKDLMKIIPMDIWDIFTLLLINHGRKVCDARKPLCHECVLNKLCPSAFKFG"
     gene            6305..6751
                     /locus_tag="VKL21_03000"
     CDS             6305..6751
                     /locus_tag="VKL21_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HMB44769.1"
                     /translation="MALSEYNKLRDISKSPEPEGGGKKSAGNIFVVHEHDARHLHYDL
                     RLEIDGVLRSWAIPKEPPEKEGIKRLAIRTEDHPLEYADYEGTIPEGMYGAGSVRIWD
                     SGEFGIEKDEENELLIELKGKKLRGKYALIKTKFRGKESWLFFKRK"
     gene            6754..7311
                     /locus_tag="VKL21_03005"
     CDS             6754..7311
                     /locus_tag="VKL21_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HMB44770.1"
                     /translation="MWQNIAKFGKKLVEQGLVESQFGNISIRRDDKMLITRSGVPLDD
                     VNKNSIVEVDIDEPSDLDRVASSETMVHRIIYKYTPALAIIHAHPMFAVIQSMLLEKE
                     TIVPINIEGEYFLHEIPVVKGAPGTSELAEYTVQALGNHKGTIVFGHGTFTIGKTLEE
                     AYFITALIEQSCKIKYYFDLAKRSG"
     gene            7488..7850
                     /locus_tag="VKL21_03010"
     CDS             7488..7850
                     /locus_tag="VKL21_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44771.1"
                     /translation="MSILDVTEDQIFHTLGKGKDPNFKPIEKVRPVISKPKKTYSNQI
                     VRPVPVVKTIQPVEPIVKPVITVPSLPQTDDSVKKLTEDMNELNNRVIGLQKMVKWYI
                     VPQFVVVLVLILLIMTKS"
     gene            7901..9076
                     /locus_tag="VKL21_03015"
     CDS             7901..9076
                     /locus_tag="VKL21_03015"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HMB44772.1"
                     /translation="MKYIVLVGDGMADYPIPELGGLTPLQAADTPNMDFIAKHGKCGL
                     AKTVPDDMPPGSDVANLSIMGYDPQKYYSGRGPLEAASIGINLQKDEIAFRCNLITEK
                     NGLLADYSSGHISSEEAGILIKAVDKELGKSCRFFAGVSYRHLLVMKQGENAQCTPPH
                     DVVGQSIEDVMPHGESSEFLISRIRDSKPILENHEINNKRKKAGKNIANLIWPWGQGK
                     APEMPLFKDMFGVWGSIISAVDLLKGIGKYAGLNIIDVPGATGYLDTNFSGKAQYALS
                     SLKERDFVLVHVEAPDEAGHMGNIEAKIKAISDFDEKVVGGMLNELGGFGDFKVMVLP
                     DHPTPISIRTHTREPVPFAIYSSTERADYVSRYDEFAAKEGIFGIMEGHRLMNLLIS"
     gene            9173..9457
                     /locus_tag="VKL21_03020"
     CDS             9173..9457
                     /locus_tag="VKL21_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44773.1"
                     /translation="MIIISKDVNLMNYYLKYSPLEDIRRIKSGYKPKNHFMFIYNIIP
                     IFIMLKLFVYGSCGNFISGIVGFEGLHKSCQVRKPKTRNANLTDIKLTAR"
     gene            complement(9509..10459)
                     /locus_tag="VKL21_03025"
     CDS             complement(9509..10459)
                     /locus_tag="VKL21_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMB44774.1"
                     /translation="MKKLHLLIQIVIGISIIAFILNRLDINQLFLVLKKTNMTYFILA
                     CLSYLFLNLLLSLRLSYLLKKIGHKIRFKNVFLSHMAGMIIGDITPGRSGYFLTPTIL
                     KKKGGIPITEGMACIFAPQGIEFILKVGGAIAAIIYLSLYSNIDEKTIIYAGIGSAIL
                     LIVGILMLMISWNNERMTSKFMGKIPFLNKFTENLSSFKDRSILIKDSLNIILLIYTI
                     GWIFAALQWYFLGKAIGINNISLFAFFLLHPLITILMFVPLTPAGFGLMEWVIILVFS
                     LFGISPALGMAFSVLVRVSILGVDLIGLKTVITASRKYGL"
     gene            complement(10469..11437)
                     /locus_tag="VKL21_03030"
     CDS             complement(10469..11437)
                     /locus_tag="VKL21_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44775.1"
                     /translation="MNKQVIIDIVKNIFTYRGYNIGSSEICDLLAEKDDEHFLIKFEK
                     DPNYNNIRYFSNMVKRYGKGIVISDSFDEKIRIFALDDGLALWDRSELEMQIGRAVLA
                     GALEKGEDRPKQKEDGDFSLVQEKEPEIREYKKTIKVMLRSVPVNIGKQDALSIAEAK
                     IGTPKNQALKFIPVWYYSYSFSIQKKYKSKIVDLSGEGEGYIHAITGENFFNKYRDIK
                     ENILMPTQNYEIKEPFLQKKDATGKALNSIIREHTKEVRLNEMIGDTIVFETKIVAPD
                     PSEINIKIDLLYVPFWEIRGKREVIEINGYDGHIMAIKLYNDAELV"
     gene            11523..>12064
                     /locus_tag="VKL21_03035"
     CDS             11523..>12064
                     /locus_tag="VKL21_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HMB44776.1"
                     /translation="MADLMFIISRIENMMYEVTFDPAKRKGKIIVNISIVNESDLKKI
                     IGLFRQAVHSGLAVSPFMKIIRTGEKIGNIKIEAGKAGIASACSITIDGVLLKAGIPV
                     KPKLGGVVEIHEGSPLRFTDVLTYDSTTIDPLDVFMSQELTSVTQMINTGSGKILANM
                     REAPMSARNRIELVLDSLVKA"
BASE COUNT         3831 a         2273 c         2543 g         3417 t
ORIGIN      
        1 gttagttccc gtaactaata acggcacagg gttcggagat acgtggggag atacctacta
       61 tttctatatg gaaattccat agacctggtt ggatccctga tccaacttct ttttttttcg
      121 gatgaaaggt ttaatatata agttttacat aatcatagtt ataatgggtt taatgctcaa
      181 gttattatta ttattattct tgctggcagg actgtcaata ctaaacgcct ctgccgctcc
      241 tgatcaggtg gttattaatt ccgcaaactg gcaggatgtt taccttggcg cgcattatgc
      301 aggctttaat aatataaaat caaattttct tgtaagcagt gaacatgcct atgtcctcct
      361 tcaaaccctt gatacaagca aggaaaacat actccttgtg gaatcggata aagcgccatt
      421 tgctttcagg tataagaccg gccttgaagg tgccggtttt attgtcgatg aaataatttc
      481 ttccgggaca gcgcttaata tagagcttgc aaaaagagca aatacaagtg atttcattat
      541 tgtagatgat tcatatgggt ataatgccat atctgttgcg ccctatgctg cccaggaaaa
      601 atttttcgtt ctttttgcca ataaggaaaa cattgatgaa ttatactcct tcctgcaagc
      661 caatggggta aacaggatta taatctatgg attttcggat atcgaagtca agagcaaatt
      721 ggctgaattc aaccccgtaa ttatcaataa tggttcaagg ttcgataata atattgaact
      781 attgaaaaga tacaagagag tccagcaggt attccttact aacggggaat ttattgaaga
      841 gggaatcatg tcagggaaag aacccgtact tttcccggga agagaaagag ttcccaacca
      901 ggtggttgaa tatatcaaga gcagtggaat aaaaacaggc gtagtcatcg gaaatgaact
      961 tacaggcgtc gctcaccaga taaaacaagc tacggatatg aatatttttt taaagtttgg
     1021 ccagggtttt gcaagtgggg gttcctctga agtaaaggct ctggatatgt tccctctccc
     1081 aaagtatgag cttgcagttg atattaattc ggttaaatat aatcgcgcaa cgaaggaact
     1141 tgaagttatt tatgaaaata agggaaatat gggagtttat ttgagttcat ctatcgaagt
     1201 attatcatcg ggcgtgagaa tagccaccct tggagacaag ggttctgtct atatggatat
     1261 tggcagcacc ctggctgttg cctatgcatc agaattgaac gggcaaaacc tgactgcaaa
     1321 attcacaaca atgttcggag aagatccagg atcatttgaa cgagcccttt ataaggaaat
     1381 gaaaattgag atgattgaag tgatagatag ctcgtcactt aatatttcaa aggtttccta
     1441 tgacaaagcc gcccataaaa taaaggtagg gatcaggaat accggggaag tcaaaacata
     1501 cgctaagacc cagataaaat tcgttatata cggcgatgaa agaattatgg tccaggataa
     1561 ggcaatgctt ttagaacctg gcgcttcgaa ggatgttgtt tttattacga ccttgagcga
     1621 taccaatatt gaggagaatc cgcaggtcaa tgtccgaata aattatggtg aaagggaaac
     1681 tgtcctgata aaagtcctgg aaggaaatta tcctctcatt ataacaggcg actatactat
     1741 actaataatt gttgtcatat tactgataat tgggaacact atccgtattc aatacaggaa
     1801 gaaacggatt aaagatcaaa aaaaatccat ggaagatagc gcggctgaaa ctgaagcaac
     1861 agagatatct aaagatacct gatagaaatc gatcgaaata ggttatttct tgcccttttg
     1921 aatccgtttt tgtaaatcat aataactata atgcttatat aattgccaat gaataatatt
     1981 atatatcttg tactcatttt attaatatga ctctgctaca gaaagcagag ttgtaaatat
     2041 gtgtgtgtaa atagatcaaa ataaagctca ggctagcctt tcataaatgg gatagtttga
     2101 actgccagaa aaggtgtgca aatgtgtacc tgaggagtga cattgataaa aagtccgacc
     2161 ataaaaataa taaacaattt atttataaaa acactattaa tgatcgcttt ctttgtatca
     2221 attgcgactg gccaggcagc ggttcaggcc aatgagggat atatagaaag gatcgatttt
     2281 gcaggtaatt cttatactga acactggctt ggattttatg gtattattaa caataatact
     2341 atgatgatcg ggaattctaa tgtgaaaaat gatactgcta tttttacggg tatctgggca
     2401 cacgagggtg attacctttt gattacaact tcaccttcac cccccttcgg attgcgggct
     2461 ggtaacatcg catcggttga tgaaataaca ggctgggggg atgactccgg ttcaaataca
     2521 ttctcatatt cctctaacta ccggatacct tattcagagg ctatactcac agatgtgcca
     2581 tcaatttaca tgtttgataa caagaagcaa tattcaaggg aagcgttgtt ttcggatagc
     2641 aatggaaatc cggtattcgc cgttccgatt gaaaatatcc ataataccag cagtcattat
     2701 tttcaattga tgctgccgga taacggaaac cttacatatt atttttttta tctgccatca
     2761 gatacacaat aggttttttc cagcgcaatc ctgatgacca tatttattgt aaagagagca
     2821 atctataagc ctataaattc tattgagtac ctatgtacca ccttgagtgc attgaatgtc
     2881 acaagaagta tcctgaaaca gaagtcatct acacctgcac atgcggtggt ctgctcgacg
     2941 ttatctatga ttattctaat atccacctca caaaaaacga tttgaaaggt ccgctttcag
     3001 tctggaaata tcgtgaattg ctgccggtaa gcagggagcc aatatccctt caagaaggcg
     3061 gaacaccgct ttatagggtt gacaggatag caaaaaatat cggactcaag gatgtttatg
     3121 tcaaacacga aggcatgaac cccaccggtt cattcaagga cagggggatg accgtgggtg
     3181 ttacaaaggc ccttgaactt ggcatgaaaa cggtcgcatg cgcctcaaca ggaaatacat
     3241 cggcatcatt ggcggtttat ggagcaaggg cgggagttcc ggctgttgtt ctccttccat
     3301 caggtaaagt agcgcttgga aaacttgccc aggcgctcat gcacggtgca aaagtattga
     3361 gtatcagggg aaatttcgac gacgcgctca aacttgttcg cgacctgtgt gacagggaag
     3421 gtttctatct tttgaactca gtaaatcctt atcgtctgga aggacagaaa acgatcgctt
     3481 ttgaaattgc agaccagctc ggatggaagg cgcccgatag gatcgtgctt cctgttggaa
     3541 atgcggggaa tatcacggcg atttataaag gattcaaaga gttaaaaaat cttggcctta
     3601 ttgacagcgt accgaaaatg actggaatac aggcgcaagg agcaagtccg gtcgtaacag
     3661 caataaagaa taatgctcgt gcaattaccc ctgaaagcca tcctgaaaca gttgcaacag
     3721 cgataaggat aggaaacccg gtaaattcaa taaaagcctt aatagccatc agggaatcgg
     3781 gaggaactgc cgaaacaata acagatgagg aaattatccg ggcccagcat gaacttgctt
     3841 acatggaagg aattggagta gaaccggcaa gtgcgtcctc gatcgcagga ctcaggaaac
     3901 tggtggagca gggtgtaata ggatcggatg aaataattgc atgtgtcacc acagggcatt
     3961 tgctcaagga ctcagaacat gtgctggcag tatgtcctaa acctatggaa atagatgcca
     4021 ctattgaagc tgtgagaaaa gcggttttct caaaataatt ggcgctaaat tgaaaaatag
     4081 tataatttta tgacactttc cagcctcata attaaagaaa tcaaacgctt tgggcctatt
     4141 tcctttcggg atttcatgga aatggcgctt tattatcccg gtcttggata ttacacatcc
     4201 cataaaaaga agattgggaa aacgggggat tattatacaa gttccaacct gactcctgtc
     4261 tttggtgaaa tgctgggaaa gcaaatacaa gaaatgtggc atattcttgg caaaaaagag
     4321 atgacggtgg ttgaaatggg agccgggacg ggtcttcttt caggagatgt gctaacatat
     4381 cttgccaata gtccggaatt attccatgac ctgaattact gcatcgtgga aaaaagccct
     4441 taccttctaa aagaacaaca agatcgtctg acccatgaaa aagtaaactg gtataattca
     4501 ataagcgagc ttaaggggat gagaggttgt attttttcaa atgaactccc tgatgcattt
     4561 cccgtacatg tggtagaaat ggaaaatgaa cttatggaag tcttcgtggg ctatgaaaat
     4621 ggtttcatcg agatcctaaa acctgcttct gatcaattga gagattattt gaaagaactg
     4681 gaggtaacat tacctttcgg atatcgcaca gaaataaacc ttgatgctat aaaatggatc
     4741 cgggagatgg gttctgcttt agaaaaagga tttgtaattt cgatagatta tggatactca
     4801 tcccgggaac tttaccagga ttacaggaac aggggcacac ttttgtgcta ttataaccac
     4861 actgtgaatg agtcgccatt tgagcatata ggggagcagg atataacatc gcatgtgaat
     4921 ttctcggcac tggatcactg ggggcagaaa aaagggcttt atctttgtgg attcacggac
     4981 caggcgcatt ttctcatggg gctgaggatc gacgaatacc tgaagaattt gcaggaaaaa
     5041 agtccggtgg actattttaa gaaaatgctt cccataaaat cgctaattat ggatatgggt
     5101 ggaacattta aagtcatgat acagaaaaaa ggggttgagt ttgctgaatt atctggtttg
     5161 aagttcccaa gtgaagctcc caaccgcagc agagatgcgg ggtaaaatca accacagagt
     5221 ccgcagagag cacagaatgt tgacgctttc ctctgtgtac tccgagctct atgtggtttt
     5281 taaaccagaa gatagaagtt tcaaaaaaag atgtgaaatt aaaccacaat acacattggt
     5341 tccgcacagc acacaacttc accggaacta tctgaagcac acaccatttc acatccgcaa
     5401 atttcacaaa tatatgtttg ccctgtttct gctttgctgg ctttctttgt cttcactttc
     5461 ttttttggtt tagctgtttt tgtttcggat ttcattgcca ttattttacc tctttcattc
     5521 caccacgaag atgacgtggt agatttggat gcagttttgt tggccataaa attttacctc
     5581 cacttatgat tataaattta acgctactct catttataaa tatcggtctg tcaaaactct
     5641 taactatctt gcatcaaata tcagggagta tggtggatga taaggttaaa aaaataatct
     5701 cattattgaa aaacgaatat cccggaataa agaccgcgct ttcttattca gaccccctgg
     5761 agttgttgat agcaacaata ctctcagccc aatgcacgga taagcaggtc aatgaagtta
     5821 caaaaaacct atttaagaaa tacaggacgc ttcaggatta tatcaaaacc ccccaggagg
     5881 aactcgaaaa ggacatatat tctacgggat tctacaggaa caaggcaaaa aacataaagg
     5941 gattatctga aattctcgtt aatgattttg attcaaaagt ccctgatacc atggaaaacc
     6001 ttcttaagct gccgggcgtg gcgcgcaaaa ccgcaaatat cgtattatcg ggagcattcg
     6061 gcaaaattga agggatagct gttgacactc atgtaaaaag gctctccggc aggctgggat
     6121 tgaccgccaa tacaaatcct gaaaaaattg aaaaagacct tatgaagatc atccccatgg
     6181 atatctggga tatttttact cttcttctga taaaccatgg acgaaaagtc tgtgacgcca
     6241 ggaaaccctt gtgccatgaa tgcgttctga ataaactctg cccaagtgct tttaaattcg
     6301 gctaatggcg ctttcagaat acaataaatt gagggatatt tcaaaatccc cggaaccgga
     6361 aggaggagga aagaaaagcg ccggaaatat attcgtggtc catgaacacg atgcaaggca
     6421 cctgcattat gacctgaggc ttgaaattga cggggtcctt cgttcctggg ctattcccaa
     6481 agagccccct gaaaaagaag gcattaagcg tctggctatc cggactgaag accatccgct
     6541 tgaatatgcc gattacgagg gaacgatacc ggagggtatg tatggggcgg ggagtgttcg
     6601 catctgggac agcggagagt ttggtattga gaaagatgag gaaaatgaac tgctcattga
     6661 attgaaaggg aaaaagctaa ggggcaaata cgccctgata aagacgaaat tcaggggaaa
     6721 agaatcctgg ttgtttttta agagaaaatg aatatgtggc aaaacattgc aaaattcggg
     6781 aaaaaacttg tggagcaagg acttgtagaa tcacaattcg gaaatatcag catccgaagg
     6841 gacgataaaa tgcttatcac caggagtgga gttcctcttg atgatgtaaa caagaacagc
     6901 atagttgagg tggatattga tgaaccttca gaccttgatc gcgttgcttc atcagaaacc
     6961 atggtccaca ggattatata caaatatact ccggcgcttg cgattatcca tgcacatccg
     7021 atgtttgcgg ttattcagtc aatgcttctt gaaaaagaga ccattgtccc gataaacatc
     7081 gaaggtgaat atttccttca cgagatccct gttgtaaaag gcgcgccagg cacatctgaa
     7141 ctcgcggaat acactgtgca ggcgcttggt aatcacaaag gaactattgt ttttggccat
     7201 gggacattca caattggaaa aacactggag gaagcttatt ttattactgc cttaattgag
     7261 cagagctgta aaataaagta ttatttcgat ctggcaaaga gatcaggtta aattctccaa
     7321 catactccgg atggaagatt cttttataat gtggaatccg acattataaa ataagagatt
     7381 gggtgctaaa acaaaaacgc ttggtgatga gattgttcac acacatcaca caataacaat
     7441 tatataattg tataacgtaa taatttcgta agagggtaca acacaaaatg tcgatacttg
     7501 atgtaacgga agatcagata tttcatactt taggcaaagg gaaagatcct aatttcaagc
     7561 caatagaaaa ggtcagaccc gtaatatcaa aaccaaagaa aacatattct aatcagattg
     7621 taagacctgt acctgttgta aaaaccatac aacctgtgga accgattgtt aaaccagtaa
     7681 taacagttcc ctcattaccc caaaccgatg attcagtcaa gaagcttacc gaggatatga
     7741 atgaactgaa taaccgggtt atagggcttc agaaaatggt aaaatggtat atagtgcccc
     7801 aattcgtagt tgtccttgta cttatacttc ttattatgac aaaatcctga tcttcaaacc
     7861 aaccgatatt ttattgtatt agcaattctt cttggaggac atgaaatata ttgtcctggt
     7921 tggggatgga atggccgatt atccaattcc ggaacttgga ggtttgacgc cacttcaggc
     7981 tgcagatacc ccaaatatgg attttatcgc aaaacacgga aagtgcggcc ttgcaaaaac
     8041 tgtacccgat gatatgccac ccggaagcga tgtggcaaat ctttctatta tgggatatga
     8101 tccccaaaaa tattattcag ggcgagggcc tctggaggca gcaagtatcg gaataaacct
     8161 gcaaaaagat gagatcgcct ttcggtgcaa tcttattact gagaaaaatg gattgctcgc
     8221 agattatagc tcaggccata tttccagtga ggaagcgggt atcctgatca aagccgttga
     8281 taaggaactt ggcaaatcct gccgattttt tgcaggcgtg agttacagac atctgcttgt
     8341 aatgaaacaa ggagagaatg cgcagtgtac cccgcctcac gatgtagtag gtcaatcaat
     8401 cgaggatgta atgccgcatg gtgagtcctc ggagttcttg atttcacgca taagggattc
     8461 aaaacccatt cttgaaaacc atgagatcaa taataaacga aaaaaagcgg gaaagaacat
     8521 agctaacctc atctggccat ggggacaggg aaaggcacct gagatgcctc tatttaagga
     8581 tatgttcggg gtttggggtt ctatcatctc agcggttgat ctattgaaag ggatcggaaa
     8641 gtatgccggc cttaatatca tcgatgttcc aggcgctacc ggatatctgg atactaattt
     8701 ttccggtaaa gcgcagtatg cactttcatc actaaaagag cgggatttcg tccttgtgca
     8761 cgtcgaagcg ccggatgaag ccggacatat gggaaatatt gaagccaaga taaaagcgat
     8821 atctgatttt gatgaaaaag tagtaggggg tatgcttaat gaactcggcg ggttcgggga
     8881 ttttaaggtc atggtattgc ccgaccatcc aacccccata tcgataagaa cgcacacaag
     8941 agaacctgtt ccctttgcga tttattcttc tacagaaaga gcggattatg tgagcaggta
     9001 tgatgaattc gcagcaaaag aagggatttt cgggattatg gaaggacaca ggctgatgaa
     9061 tttgctaata tcttaataga aatgttttaa tctttttgga ttgaaatttc atactttaat
     9121 aagttttttt gatgacgtaa tagtacagta atatttatat gttattagga ttatgattat
     9181 aatatcaaaa gatgtgaatc ttatgaacta ttatctcaaa tattcaccac ttgaagatat
     9241 caggagaatc aagagcggtt acaagcccaa gaaccatttc atgttcatat ataatattat
     9301 tccaatattt attatgttaa agctgtttgt ttatggatcg tgcggaaatt tcatttcagg
     9361 tattgtcggt tttgaagggt tacacaaaag ctgccaggtt cggaaaccta aaaccagaaa
     9421 cgctaatcta acggatatca aattaaccgc aagataggga tttttttata tagtggggtc
     9481 aagtggtaga gaaaactacc cgcctggttc acaaaccata ttttcttgat gcggtaatga
     9541 cagtctttaa cccgatcaaa tcaacaccta aaatgctgac tctcaccagc accgagaatg
     9601 ccattccaag agccggtgaa atcccaaaca atgaaaatac gagaataatc acccactcca
     9661 tcagcccaaa tccggcagga gtcaatggga caaacatcag gatcgtaatg aggggatgaa
     9721 gcaggaagaa agcgaacagc gaaatattat tgatcccaat agcttttccc aaaaagtacc
     9781 actgcagcgc tgcgaagatc catccaattg tatagattag cagaataatg ttcaagctgt
     9841 ctttgatcag gatgctcctg tctttgaaag aagagagatt ttcagtaaat ttattaagaa
     9901 atggaatctt gcccatgaat tttgatgtca ttctttcatt attccaggat atcatcaaca
     9961 tcaatatccc gactatcagg agaattgcag accctatccc ggcataaatg attgtttttt
    10021 catcaatatt tgaataaagc gataaataaa ttatagctgc aatagccccc cctactttaa
    10081 gtataaattc aattccctgc ggagcaaaaa tacatgccat cccttctgta attggaatac
    10141 cgcccttttt ttttaagatc gtaggtgtta aaaaataacc gcttctccct ggagttatat
    10201 caccaatgat cattcctgcc atatgcgaaa gaaaaacgtt tttaaatctt attttatgcc
    10261 cgattttttt cagcagatag gataatctta atgacagtaa cagattaagg aacaggtagg
    10321 agagacatgc caggataaaa taggtcatgt tggttttttt aagaacaaga aataactgat
    10381 taatgtccag tctattcaaa ataaatgcaa taatggatat tccaataaca atttgaatta
    10441 ataaatgaag cttcttcata atactccttc aaacaagttc agcatcatta taaagcttga
    10501 tcgccattat gtgtccgtca tacccattta tttctattac ttcacgtttg cctcttatct
    10561 cccagaaagg aacatacagg agatctatct taatatttat ttcagatggg tccggtgcaa
    10621 caatcttagt ttcaaagaca atagtatcgc ctatcatttc attcaacctc acttcttttg
    10681 tgtgttccct gataatggaa ttcagggctt ttcctgtagc gtcctttttt tggagaaaag
    10741 gctcctttat ctcataattc tgggtgggca tcaggatatt ttccttgatg tccctgtatt
    10801 tgttaaagaa attctctccc gttatggcat ggatatatcc ttcaccttcg ccggaaaggt
    10861 caacaatttt tgatttgtac ttcttctgga tactaaaaga ataactataa taccagaccg
    10921 gtataaattt caatgcctgg ttttttggcg tcccgatttt tgcttcggct attgataaag
    10981 catcctgttt tccaatattt acaggtactg aacgcaacat tactttgatg gtctttttat
    11041 attcccttat ttcaggttct ttctcttgca ctaatgaaaa atcaccatct tctttttgtt
    11101 ttggtctgtc ttcacccttt tcaagtgctc ctgccagcac tgctcttcca atttgcattt
    11161 caagctcgct cctgtcccaa agtgcaagtc cgtcatcaag cgcgaagatg cggatctttt
    11221 catcaaaaga atctgatatg actattccct tgccgtatct tttcaccata ttcgaaaaat
    11281 acctgatatt attgtaatta ggatcttttt caaatttgat caaaaaatgt tcatcatctt
    11341 tttcggcaag caggtcgcag atttcagaag aaccgatgtt ataaccgcgg taagtgaaaa
    11401 tattttttac aatatcaatt ataacctgtt tattcatgaa taccacttct tacatgtcta
    11461 aaatacttaa tctttcggta tgtttataac tcattgtaat tatacatgat tgctgaatta
    11521 atatggccga tttaatgttc ataatctcaa ggatcgaaaa tatgatgtat gaggttacct
    11581 ttgaccctgc aaaaagaaaa ggtaaaataa ttgtaaatat ttcgatagta aatgaatctg
    11641 atttaaagaa aatcatcggt cttttcaggc aggcagtgca tagcggactt gcagtaagcc
    11701 cttttatgaa aataatacgg acgggagaaa aaatcggcaa tattaagatc gaagcaggca
    11761 aggctggaat agcctcagcc tgcagcatta ctattgatgg agtgctgcta aaagccggaa
    11821 tacctgtaaa accgaaattg gggggagtcg ttgaaatcca tgaaggctca cctttgagat
    11881 ttaccgatgt gctgacctac gatagtacca cgattgatcc tcttgatgtt tttatgtccc
    11941 aggaactcac atctgtaacc cagatgatca acacaggttc aggaaaaata ctggcaaata
    12001 tgcgtgaagc tcccatgtca gcccgcaaca ggatagaact ggtgctggat tctctcgtta
    12061 aagc
//
LOCUS       DATGBN010000133         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_243425, whole genome shotgun sequence.
ACCESSION   DATGBN010000133 DATGBN010000000
VERSION     DATGBN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243425"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VKL21_03040"
     CDS             <1..925
                     /locus_tag="VKL21_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB44777.1"
                     /translation="CWYNGPIENCDRQSRKECVGCIFKPKNSSGANIKLGDVIGLDKA
                     KEALNRRMVEVLKHKKKLVERNAGVSKGILFFGFPGTGKTLLAKAAANELMEDNPSIS
                     FDTISIGDIGSSSVSTSDRKVKAMFAKWRNRDDREWIILMDEADAIIPDRTKVKSVLT
                     IERVSAMLREIDGVQDTDNVYFICTTNRPFSIDPAFMRSGRIEDRVFIDLPNDKERLA
                     LLEFYLKDIDFQGKDIKLPFIAKRTENYCGADFKRMRNELILTEEIKKESNTQYKLTD
                     QDMTVAISKCRRPIGKNIENMRQEYLLFADD"
     gene            complement(1162..2172)
                     /locus_tag="VKL21_03045"
     CDS             complement(1162..2172)
                     /locus_tag="VKL21_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44778.1"
                     /translation="MKQRPKGFLDPYIIDSLVDRGFRARWHEIKEDLLLKLDVDDKTF
                     SAALKRLEDLGIIRKDNDIYYLLPKLPIPGEPFQIIKGYNYIQTLKIWADEISVQENT
                     GKKTEMLAKYLFRTWANITSLYLNMLEKYIETQSEHLPEKDTEIRARKRSEEYLDKEM
                     MVLAALMKKTARLFVPPKKPRIPILTGKTKVRMKISKQEINMARRIILATLARELPEE
                     TIIQVMTHNCSRGIDLTGDAFGFKDSIFSWNEIPGKDNEKLIEFLKEDFDINWVKIVK
                     MAKIEKVDDGTTIRISTEKNSLSLKLNDDKTRVTLEIDDGRTHEFTVMKEKGKRNIYY
                     RN"
BASE COUNT          851 a          511 c          563 g          788 t
ORIGIN      
        1 gtgttggtat aatggaccaa tagagaactg cgataggcaa tctcgtaagg aatgcgtcgg
       61 atgcatcttt aaaccgaaga acagctcagg agcgaacata aaactgggag atgttatcgg
      121 gctggacaag gcgaaagagg cactgaacag gcgaatggtg gaggtactca aacacaaaaa
      181 gaaactagtt gaacgcaatg cgggtgtatc aaaagggata ttatttttcg ggtttcccgg
      241 caccgggaag accctgctcg caaaagcagc ggcaaatgag cttatggaag acaacccctc
      301 catttccttc gatacaattt caattgggga tataggaagt tccagtgtca gcaccagcga
      361 ccgtaaagta aaggccatgt ttgctaaatg gcgtaacagg gacgatcgag agtggattat
      421 tctcatggac gaggctgacg caatcatacc cgacaggacg aaagtgaaat cagttcttac
      481 aatagaacgg gtgtcagcaa tgcttcgcga aattgatggc gtgcaggaca ctgataacgt
      541 gtattttata tgcactacca acagaccgtt cagtatcgat ccagcattca tgcgcagcgg
      601 caggatagag gacagggtgt ttattgattt gccaaacgac aaggaacgct tggcactgct
      661 ggagttctac ctcaaagata ttgacttcca gggtaaagac attaaattgc cattcatagc
      721 aaaaaggact gagaactatt gcggagcgga tttcaaacgg atgcggaacg aactcatcct
      781 gaccgaggaa attaaaaagg agagtaatac ccagtacaag ctgactgacc aagatatgac
      841 tgttgccatc agcaagtgtc gcaggccaat aggcaagaac atagagaaca tgagacagga
      901 atacctcctt ttcgctgatg attaactttg taactgtgta aacagacctt ctttacctcc
      961 tatctttctg agcttacaaa gtgcgggaaa tgtaggtggg tttagacagt tgggtaggga
     1021 tttcaggagt gcggcggtgt atgtacagga cagtcaggca ggcatcttat ttgaaaggat
     1081 attggaaatt aataccggag cctcccgtcg gtgagagaca tcaaggcaga cggtgctgat
     1141 gtcatcagca gtcgtttggc gttaatttcg atagtatatg ttccgcttac ctttctcctt
     1201 cattacggta aattcatgag ttcttccatc gtcgatttct aatgttaccc tggttttatc
     1261 atcgtttaat ttaagcgaaa gagaattttt ttcagtagat attcttatgg ttgtgccgtc
     1321 atcgaccttt tcaatttttg ccatttttac tatttttacc caattgatat caaaatcttc
     1381 tttaagaaat tctatgagtt tttcgttatc ttttcctgga atttcgttcc agctaaatat
     1441 agaatcttta aagccaaaag catcccctgt taaatctatg cctcttgaac aattatgagt
     1501 catcacctgt attatcgtct cttccggaag ttctcttgcc aaagttgcga gaattattct
     1561 tctcgccatg ttaatttctt gttttgagat cttcattctt accttggttt ttcctgttaa
     1621 aatcgggatt cttggtttct ttggaggaac aaatagtcgc gcagtttttt tcataagagc
     1681 ggccaaaacc atcatttctt tatcaagata ttcttccgac ctttttctag ctcgaatctc
     1741 tgtatccttt tcaggtaaat gttcagattg cgtctcaatg tatttctcaa gcatattaag
     1801 ataaagagag gtgatatttg cccaggttcg aaacaaatat ttagctaaca tctcggtttt
     1861 cttgcctgta ttttcttgaa cgcttatttc atctgcccag atctttaaag tctgtatata
     1921 attatagccc ttgataattt gaaaaggttc gcctggaatt ggaagtttag ggagaagata
     1981 ataaatgtca ttatcttttc ttataattcc cagatcttcg aggcgtttca aagccgccga
     2041 aaacgtcttg tcatcaacat ccagtttcaa gagtaaatct tctttaattt catgccatct
     2101 tgcacgaaat cctctgtcta ctaagctatc tatgatataa ggatcaagaa atccttttgg
     2161 tctttgtttc atataatcat ctttttttac ttacttactg ttcggaaaat tatctatatg
     2221 tatagcccat atttaacctt gctgtgacat tgattaagaa tgtcccggag taaagatgaa
     2281 ataaaaaaaa tacggaaaag aggatgataa cacgataaag gttgaacaat aaattatttc
     2341 aaatagccaa aacctgggct aaccccttgg ggggtcaaag tagacatcat aattgtctga
     2401 aaaaggtgac ggacaatcgc atagcaagct cggttggaat aaaagacagg tagtgtagga
     2461 tataaaataa aataatatat aattaaattt atggttgcac tgagagctaa aagaagcccc
     2521 cagcacataa agcgccgcta cttataacct tcgattttca atggacggaa tacgacaatg
     2581 aacaaaatta gaggattcac tctacagagg aagcatgata aaaaacaata taaatataga
     2641 aataaacgaa acaaaacaag tcaaaaatca actcaaggag gaaacacaaa atatgagcat
     2701 aacaggaaac ggt
//
LOCUS       DATGBN010000134         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_515350, whole genome shotgun sequence.
ACCESSION   DATGBN010000134 DATGBN010000000
VERSION     DATGBN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515350"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VKL21_03050"
     CDS             <1..308
                     /locus_tag="VKL21_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-ribbon containing protein"
                     /protein_id="HMB44779.1"
                     /translation="SVAKTHISTNTVQISPEASNFIREIDELLGIKDEKPELKNEPET
                     ETKEVGNRIESIRILSPGQYELNLESLLERKEIVMALKEDGTYIVNLPSVFKKKKGH"
     gene            complement(305..742)
                     /locus_tag="VKL21_03055"
     CDS             complement(305..742)
                     /locus_tag="VKL21_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44780.1"
                     /translation="MKNPIPAFFIILILVSFLPLSGGAQGTRVFEITSLIIKFQKTDA
                     EFTVNYDIGTIPKMYILLMGGKSIEPKMKELFSNFDYEIVKMDQEKAILQVKNISRFE
                     KGYYLHDSIGFGTRIDIMTIYIPGDPQPAQYFGLNATKNTFYS"
     gene            846..1775
                     /gene="mdh"
                     /locus_tag="VKL21_03060"
     CDS             846..1775
                     /gene="mdh"
                     /locus_tag="VKL21_03060"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMB44781.1"
                     /translation="MPKITIIGAGAVGATTAQRIAEKEFGDVVLTDIVEGLPQGKALD
                     LLEAGPLFGYDSNIMGTNDYEDIKGSDVVVITAGIARKPGMTREDLLKINTKIITDVS
                     QNIKLHAPDSVVITVTNPLDIMTYVAMKILGFEHNRVFGMSGVLDSGRFSTFIALELG
                     CSVRDINAMVLGGHGDTMVPLPRFTTVSGVPITELMSESTIKRLVERTINGGAEIVNM
                     LKTGSAFYAPSAAVTNMVEAVIKDTKRILPACAYLNGEYGKKDIYLGVPVKLGRKGIS
                     QIIELELTREEKKALDISSDAVRSGIASLNALR"
     gene            1898..2470
                     /locus_tag="VKL21_03065"
     CDS             1898..2470
                     /locus_tag="VKL21_03065"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HMB44782.1"
                     /translation="MTALSTLAVILIGVGIILFFASNWELIPDPIKIVLLLAATFSTY
                     YTGYVLRFEKQNYPRAGHALIFLGSILVGASIFLIGQTFNISVEAHWLVLLWFIVISP
                     MGYVFDSRPTLGLNILTFTYWLALSVNPGYGSMSSTLLLYLLFGIALYSIGQLHELTY
                     KWARFRMIYKGFGIFFILISYFYFSDAGLY"
BASE COUNT          798 a          526 c          588 g          759 t
ORIGIN      
        1 gatcagttgc aaaaacccac atcagtacaa acacggtaca gatatcacca gaagcttcga
       61 atttcataag agaaatcgat gaactccttg gaatcaagga tgaaaagcct gaattaaaga
      121 atgaacctga gaccgaaaca aaagaggttg gaaaccggat tgaatctata aggatattgt
      181 cacccgggca atatgaattg aaccttgaat cgttgctgga aagaaaagaa attgtcatgg
      241 cgctcaagga agatggtaca tacatcgtaa atttgccttc tgtcttcaag aaaaagaagg
      301 gtcattagct ataaaatgta ttttttgtgg cattaaggcc aaaatattgc gcaggctgcg
      361 ggtccccggg gatgtaaatg gtcataatat ctattcgtgt cccaaagccg atcgaatcat
      421 gtaaataata acctttttca aaccttgaga tgtttttcac ctgcagaatt gccttctcct
      481 gatccatttt tacaatctca taatcgaaat tcgagaaaag ttctttcatc tttggctcga
      541 tgctttttcc tcccataaga agaatataca ttttcgggat cgtgccaatg tcgtaattca
      601 ccgtgaattc agcatctgtc ttctggaatt ttattatgag cgatgtaatc tcaaaaacac
      661 gggtgccttg cgctccccct gacagcggga gaaatgatac caagattaaa ataataaaga
      721 atgcagggat cggatttttc atcagggatt tgttatgcac tctgcggata tatcttttgc
      781 gtctcatgca taaccattat cgtccatgaa acaccttctt acaaaaaaaa tcaaggtgac
      841 attaaatgcc aaaaatcaca attatcggcg ccggagcagt cggtgcaaca accgcgcaaa
      901 gaatagcaga aaaagaattc ggggatgtgg tcctgacaga tatagtcgaa gggctgcccc
      961 agggcaaagc tcttgacctt ctggaagcgg gtccgctttt cggatatgat tcaaatatca
     1021 tgggaacgaa cgattacgag gatatcaaag gctcggatgt ggttgtcatt acggctggaa
     1081 tagcgcgaaa acctggaatg acaagagaag atctgcttaa aataaataca aaaataatta
     1141 ctgatgtttc gcagaatatc aaacttcatg cacctgattc tgtggtgata accgtcacga
     1201 acccgcttga tataatgacg tatgtggcca tgaaaatcct gggttttgaa cacaaccgtg
     1261 ttttcgggat gagcggtgtc ttggattccg gcaggttttc cacatttatc gccctcgagc
     1321 ttggctgctc agtccgggat attaatgcga tggtgcttgg agggcatggt gatacaatgg
     1381 tgcctcttcc caggtttact acggtatccg gagttcccat cacagagctt atgtcagaat
     1441 caaccatcaa gcgccttgtg gagagaacca tcaacggtgg cgcagagatc gtaaacatgc
     1501 taaaaacagg aagcgcattt tatgctccat cagcggctgt gacaaatatg gttgaagctg
     1561 tgataaaaga tacaaaacgt attcttcctg cctgtgcgta tttgaatggg gagtatggaa
     1621 agaaggacat ctatctcggg gtcccggtca aactgggaag aaaaggtatt tcgcagatca
     1681 tcgaactgga actgacccgc gaggagaaaa aagccctgga tatatcttca gacgctgtca
     1741 ggtcaggaat tgcatccctg aatgctttac gttaaatggt tatcattctg gatttcacaa
     1801 aatcacaaca agatttaatg ctcataatga taatacattg ttcattcaaa aatgaaaaga
     1861 aggtattgaa aaaaagacat acaaacccgg aaatgtaata acagctcttt caacgcttgc
     1921 tgtaatactg atcggggttg ggataatact attttttgcc tcgaattggg agctcatacc
     1981 ggacccaatt aaaattgtgc tattgctggc agctactttt tcaacttatt acacaggata
     2041 tgtactgaga tttgaaaaac aaaattatcc cagggccggt catgcactta ttttcctggg
     2101 ttcgatactg gtaggcgcca gcatatttct tatagggcag accttcaata tcagcgtgga
     2161 agcacactgg cttgtccttc tctggttcat agtcatatcc cctatgggtt atgtttttga
     2221 ttcaaggccc actcttggat taaatatcct tacttttact tactggctgg cgctgtccgt
     2281 caatccggga tatggcagta tgtcatcaac gcttctgctt tacttgctgt tcggtattgc
     2341 gctttacagt atcggccagt tgcatgaact gacgtataaa tgggcaagat tcaggatgat
     2401 atataaaggg tttggaattt tttttatcct tatttcatac ttttatttca gtgatgcagg
     2461 tttatattaa ccacctgcag ggaatgcttc ccaagggtct cggacttata atcgggggga
     2521 tattcctgtt attctttgca acatatcttg agaaaaagag gaggactctt cttaatgcta
     2581 tgaagggagg agactaatgg acaggaaaac attcatcaaa gctatgctta ttcctgcagt
     2641 aatactgctc gggtttattg gttataatta t
//
LOCUS       DATGBN010000135         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_515351, whole genome shotgun sequence.
ACCESSION   DATGBN010000135 DATGBN010000000
VERSION     DATGBN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515351"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VKL21_03070"
     CDS             <1..249
                     /locus_tag="VKL21_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMB44783.1"
                     /translation="FYGSNVNNWNTTKQSGTNIVGGLYLGGNYWAYPNGTGFSQTCSD
                     ANKDGICDSIYELYAGNKDFLPLKKRLTGTISGFVRRW"
     gene            complement(814..1491)
                     /locus_tag="VKL21_03075"
     CDS             complement(814..1491)
                     /locus_tag="VKL21_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HMB44784.1"
                     /translation="MQDCLIETGLHNSFFFISEGSKRIITKNLAPGIRVYDEKLINIE
                     NEEYRTWNPFRSKLAALVIKGSSILIKKNYNVLYLGAANGTTASHVSDIVADGTVFAV
                     EFSPRAMKDLINVASSRMNLVPILSDAANPDSYQNMVPGVDVIYQDVTQRDQAGIAIR
                     NAELYLKKDGILVLIIKARSIDSTRDPGDVAHMEVRKLERIFKVKEIVNLEPFHRDHF
                     AAVAQRI"
     gene            complement(1472..2593)
                     /locus_tag="VKL21_03080"
     CDS             complement(1472..2593)
                     /locus_tag="VKL21_03080"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HMB44785.1"
                     /translation="MTQYKIPVIPGDGIGPEIIREGRKVLDAAGEKFGFDVEWIEYPH
                     GADHYLETGELISEDTLKDLSKYRAIYFGSIGDDRIKPGILEKGILLNIRFYFDEYVN
                     LRPVKLLEGVWTPLKDKTSKDIDFVVVRENTEDFYIGIGGRAKKGNSKKTLEVVRNMY
                     NVKFGLDITSDSDEIGYQLGLISKEGTSRVIRYAFELAKNRNRHLSSVDKANVLSDIY
                     GLWREEFTSIASGYPEVKTDFNFVDAVTMWFVKNPEFFDVVVTPNMFGDIITDLGAMI
                     QGGLGLAPGGNINPEGTSMFEPIHGSAPKYKGQNKMNPIATIWAGALLLDQLGEKEAS
                     IEIVRAIEKNIVNAQVKTYDMGGSNTTSEVGDDIARLLN"
     gene            complement(2603..3784)
                     /locus_tag="VKL21_03085"
     CDS             complement(2603..3784)
                     /locus_tag="VKL21_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44786.1"
                     /translation="MAFVQTLMNSKKHIDEVDLEIDIGKNVALRPRLEKMQFMESGVE
                     ANLYRMLYSQGPGNGTALFMPFDQKSSEHGPGHEFLWENDSEADEINLRGKGSADMRS
                     IAELVNCGNFSGYVLHPGNVRQFKHLFRPDIPLIYKIDGHISQPQSAGFMSTVGTIEE
                     ALKYGACAIGLTIYPGGEYIKADLERAAGIIRKAHDAGLVAVVWAYARGQGLDEKTKF
                     KDKEGEFQGVTQSDSLYWTHYAVSIASGLLGADIVKTKYPAKVKTDNRRAYDSHIESL
                     AKKNSSMIAYKYLEPENAESTLTSEQEIFRASIVVQATPHSIVIFSGGPKLPGNPTES
                     LVKQTEIVMKAGAEGGIYGRNIWGLPVKEGLKAACAVNEVISKPEFHRKLTESRFAGY
                     E"
BASE COUNT         1063 a          961 c          779 g         1267 t
ORIGIN      
        1 ttctatggtt caaatgttaa caattggaac actacgaagc aatcaggcac taacatcgtt
       61 ggaggactat acctcggtgg caactattgg gcatatccga acgggacagg cttcagtcag
      121 acctgttcag atgctaataa agatggaata tgcgattcaa tctatgaact ttatgcaggc
      181 aataaagact tcttacctct taagaagcgg ctcaccggta cgataagtgg atttgttcga
      241 aggtggtaat cagggtattt ccgggctcta cgatttacat atgctccttt ttcgagacaa
      301 atcaatttag cccagtacaa acgttcgtgt caccagacag cacttcatat cagaagtcgt
      361 ccccatattt agtgacagct tcccgtaatg tttgacatgg ccataaaaca atagagccag
      421 tccacgataa tctccggttg catcttatcg agccactctt tctctgacaa tgcagtagct
      481 gcaaaattgt tgaggattgt ttactgggtc ttgaagaaga ataacaagga gtatcaaaca
      541 caagtaaatt aagtttaatc tgttgaatag ggaactgacc tcacaaaatg atagccaatg
      601 ctattttttt ttatttcttg caggtatgat tggtgtattc tataactaat atcatattaa
      661 actccgccat cggcggcgcc tcaccggggt ctggggtcgc accccagcgc cgttaagata
      721 atgttgaccg aaagttttaa tagtacgctc atttgcggtt ccatttattc atacttcata
      781 gctttgaaca catcttaaaa ccatacatag tattcatatt ctttgagcca cggccgcaaa
      841 gtgatcgcga tggaatggtt caagatttac tatctctttt actttgaata ttctttcaag
      901 ctttcttact tccatatgag ccacatcccc cggatctctt gttgaatcaa tacttcttgc
      961 cttaataatt aagacaagga taccatcttt tttaaggtat aactctgcat tcctgatagc
     1021 tatcccggcc tgatcccttt gcgttacatc ctgatatatt acgtccacac ctggaaccat
     1081 attttgatag gaatccgggt ttgcagcatc ggacaatatg ggaacaaggt tcattcttga
     1141 agaggcgaca ttaataaggt ctttcattgc ccgtggtgag aattcaactg caaaaacagt
     1201 cccatctgca acgatatcgg aaacatggct ggctgttgtt ccatttgccg ccccgagata
     1261 caggacatta taatttttct ttattaatat agatgaacct ttaatcacca gggctgcaag
     1321 cttgcttctg aacgggttcc aggtgcgata ttcctcgttt tctatattga tgagcttttc
     1381 atcgtaaact cttattccgg gagcaaggtt ttttgtaatt attctttttg aaccctctga
     1441 aataaaaaaa aatgaattat gcagtccggt ttcaattaag caatcttgca atatcgtctc
     1501 cgacttccga tgtggtattt gacccgccca tatcgtatgt tttaacttgc gcgttaacga
     1561 tatttttctc gatagcccgt acgatctcta tggaagcttc tttttcacca agctggtcaa
     1621 gaagcaacgc ccctgcccag attgtggcga tgggattcat cttgttctgt cccttatatt
     1681 ttggcgcgct gccgtggatg ggttcgaaca tgcttgtacc ttcaggatta atgttgccgc
     1741 ccggagcaag gccaagacct ccctgtatca ttgcccccag gtctgtaatt atgtccccga
     1801 acatattggg cgttaccacc acatcgaaaa attcagggtt cttgacaaac cacatggtta
     1861 cagcatccac aaaattgaaa tctgttttga cctcgggata tcctgaggca attgaagtga
     1921 actcctcgcg ccataatcca taaatgtcag agaggacatt tgccttgtca actgaagaga
     1981 gatgcctatt cctgtttttt gccagttcaa atgcataacg gatcaccctg cttgtgcctt
     2041 ctttgcttat cagaccgagc tggtagccga tttcatcact gtcagaagtg atatcaaggc
     2101 caaatttcac attatacata tttctgacaa cttcaagtgt tttcttgcta tttcctttct
     2161 tagcacgccc acctattccg atatagaaat cttcggtatt ttcccgcacc actacaaaat
     2221 caatatcctt tgaagtctta tcctttaacg gggtccatac tccctcaagc aatttcacgg
     2281 ggcgcagatt gacatattca tcaaaataga acctgatatt caggagaatt cccttttcaa
     2341 ggattccggg ctttattcta tcatcgccta ttgatccgaa atagatcgcc ctgtattttg
     2401 aaagatcctt tagcgtatct tcggatatca actcgcccgt ttcaagataa tggtcagctc
     2461 catgcggata ttcgatccat tctacatcaa aaccaaattt ttcaccggca gcgtcaagga
     2521 cttttcgtcc ttctcttatg atttccgggc cgatgccatc ccccgggatc actggtattt
     2581 tgtactgtgt cataattatc ctctattcat acccagcaaa tctagattct gtgagcttcc
     2641 tgtggaattc aggtttgctg ataacctcat tcaccgcaca ggcagcctta agcccctctt
     2701 tcactggcaa cccccatatg ttccttccgt aaatgcctcc ctctgctcct gctttcatca
     2761 cgatttccgt ttgttttacc aggctctcgg tgggattgcc gggaagcttc gggcctcctg
     2821 agaaaataac aatggaatgg ggagttgcct gcacaacaat gcttgccctg aatatttcct
     2881 gctctgacgt caatgttgat tctgcattct caggttcaag atatttgtaa gctatcatgc
     2941 ttgagttctt ttttgcaaga gattcaatat gtgaatcgta tgctcttcgg ttgtcggtct
     3001 ttacctttgc gggatatttt gtcttgacaa tatccgcccc caataatccg gaagctatag
     3061 aaacagcata atgggtccag tacaggctgt ccgactgtgt gacaccctgg aattcccctt
     3121 ctttgtcctt gaatttggtt ttctcatcaa ggccctgccc ccttgcataa gcccatacca
     3181 cagcaacaag acctgcgtca tgggcctttc ggataattcc agctgctctt tcaaggtcag
     3241 cttttatata ctccccgcca ggataaatag tcaaaccgat cgcacaggcg ccgtacttga
     3301 gagcctcttc tattgtgcca actgttgaca tgaatccagc gctctggggc tgtgatatgt
     3361 gaccgtcgat cttgtatatc aaaggaatat caggcctgaa taagtgtttg aattgtctca
     3421 cattaccggg gtggaggaca taaccggaaa aatttccgca gttcacaagt tccgctattg
     3481 agcgcatgtc tgccgagcct tttcccctga gatttatctc atctgcctcc gaatcatttt
     3541 cccaaaggaa ttcatgaccg ggaccgtgtt ctgagctttt ctggtcaaaa ggcatgaaaa
     3601 gggctgtccc gtttcctgga ccctgcgaat aaagcatgcg gtaaagattg gcttcaaccc
     3661 cggattccat gaactgcatt ttctcaagcc ttggccgcaa agccacattt ttcccaatgt
     3721 caatttccag atcaacttca tcaatatgtt tcttagaatt cattaatgtt tgaacgaatg
     3781 ccataaaaat tccctcattt gcatattttt ttcttcttta aatgtttcag gatattaaaa
     3841 atctaacgtt ttcttaatgg caaaaatatc ccgcaaatcc agtgagaagc ccgaaaaata
     3901 tctccttgat cttgtggtta ttaactgaag ataaaaaaag tctcaattca taagtaactt
     3961 ccttatatca tgaatgcaaa taagtccctc catgcaaata agtttctcat ctcttgctct
     4021 tgttaataac ccgtttgact gggcttatgg ccttgaagag ctgggattta
//
LOCUS       DATGBN010000136         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_553875, whole genome shotgun sequence.
ACCESSION   DATGBN010000136 DATGBN010000000
VERSION     DATGBN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553875"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1119
                     /locus_tag="VKL21_03090"
     CDS             34..1119
                     /locus_tag="VKL21_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44787.1"
                     /translation="MQLQVKAVEDYLKELYGKSKVTKIGELGAIPVEVEEGIKGFGYG
                     KPYLIEFEACGKAHSVVLSSMRVQKGFGHDHFSDRAQILIWQNSVFNNLPKHVRSLDT
                     GYFTKDGKLFSAGKAEEYFILMEKIEGKEYFLDLERIKKEGKLTSLDMDRTRALSSYL
                     ADIHANKHDDRELYVRKIRDLVGHGECIMGLMDSYPDNFDFVSHNELCNIEKKCVDWR
                     YKIKSKINRLCMTHGDFHPWNIMFREGTDFTALDRNRGEWGEAADDVSSITMNYLFYS
                     LQKYGELKGPFKEMYELFFNIYLEKTGDMELLNVIQPFYVFRSVVVASPIWYPNLDPV
                     VRTKLFNFINNILDIEKFDYKKVNSYL"
     gene            1127..1678
                     /locus_tag="VKL21_03095"
     CDS             1127..1678
                     /locus_tag="VKL21_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HMB44788.1"
                     /translation="MAFAVWFTGLPGSGKTVIASRVAAILRNEGIEVKILQLDEIRKV
                     LTPDPKYTDDERNIVYASLAYMAKLVTECGVNVFIDATANKRKYRNAARKLIPQFAEV
                     YIHCPLRVCMEREVHRRAAFSPKGIYEKSTRADANVPGVNVAYEEPFNPIVIIDSDRM
                     KPDNSALTASDAIISFFREKHEN"
     gene            1668..2468
                     /locus_tag="VKL21_03100"
     CDS             1668..2468
                     /locus_tag="VKL21_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMB44789.1"
                     /translation="MKIETLVEIGVEISEVISSFIEKNEDYGEMIVQRPKDITRKMDM
                     AAETALDTALLKRGLSARIISEELGDRIIGKDPDFMLVVDPIDGSTNATCGIPFFCTS
                     LAYTDKTEIATFDDISMAVICDIKGNTYYAQKGKGAYLNGKEITGEKRGIRPKPVVSV
                     YSYGVPIVPPGLIELEKSIIVRALGSIALDMCFVADGTLDGIIDTRGLVSGYDIMASA
                     LILKESGGTLTDLKGNNLTNNVQVTGLSIAGTRKSELHDKIIRMLEVK"
BASE COUNT          761 a          472 c          609 g          677 t
ORIGIN      
        1 taaatacctc cattcctgga ctatgggaac gtgatgcagt tgcaggttaa agccgttgag
       61 gattatttaa aagaactcta cgggaaatca aaagttacaa aaatcggaga gcttggcgca
      121 attccggtcg aggttgagga aggaataaaa ggttttggct atggcaagcc atatctcatt
      181 gaatttgaag cctgtggaaa agcgcattca gtagtacttt cttcaatgcg cgtacagaag
      241 ggattcggac atgaccattt ttccgacagg gcgcaaatcc tcatctggca aaattcggtt
      301 tttaacaatc tcccaaaaca cgtaagatcg cttgacacag gatacttcac gaaagatggg
      361 aagctctttt cggcaggaaa agcggaagaa tattttatct taatggaaaa gatcgaggga
      421 aaagagtatt ttcttgacct tgaaagaata aaaaaggaag gaaaactcac atctcttgac
      481 atggacagaa cacgcgcatt atcctcgtat cttgctgata tccatgcaaa caaacatgat
      541 gaccgggaat tatatgtcag gaaaataagg gatcttgtgg gacatggtga atgtatcatg
      601 ggattgatgg acagttatcc tgacaacttt gattttgtga gccataatga actctgtaat
      661 attgaaaaga aatgtgtgga ctggcgctat aagataaaga gcaagataaa ccgcctctgt
      721 atgacgcatg gggacttcca tccatggaac atcatgttcc gtgagggcac ggatttcaca
      781 gcgcttgaca ggaaccgggg tgaatggggt gaggcggcag atgatgtttc ctcgataaca
      841 atgaactacc tgttctattc tctccagaaa tacggggaac taaaagggcc atttaaggaa
      901 atgtatgaac tcttttttaa tatctacctc gaaaaaacag gtgacatgga gctattgaac
      961 gttatccagc ctttttatgt cttcagatcc gttgtggtgg caagcccgat atggtatccc
     1021 aatcttgacc ctgttgtaag gacaaagctc tttaatttca taaataatat tctggatatc
     1081 gaaaagtttg attataagaa agtgaattct tatttatagg attattatgg cttttgcagt
     1141 gtggttcacg ggtcttcccg gaagcggtaa aacagtgatc gcatctcgag tggctgccat
     1201 tctcaggaat gaggggatcg aagtaaagat cctccagctt gacgagatca gaaaagtcct
     1261 taccccggac ccgaaatata ctgatgatga gcgaaatatc gtttatgcat cccttgctta
     1321 tatggcaaaa ctcgtgactg aatgcggtgt gaatgttttt atcgatgcga ctgcaaacaa
     1381 gagaaagtac cgcaatgcag cgcgaaaact tatcccgcaa tttgctgaag tttatatcca
     1441 ttgtcctctc agggtttgca tggagcggga agtccacagg agggccgctt tctcacccaa
     1501 agggatatat gaaaaatcaa cacgcgcaga tgccaatgtc cctggcgtca atgttgctta
     1561 cgaggaaccc ttcaatccta ttgttattat cgacagcgat agaatgaagc cggataatag
     1621 cgctttgacc gcatctgatg ctatcatttc ttttttcagg gaaaagcatg aaaattgaga
     1681 ccctggttga gatcggtgtt gagataagtg aggtcataag ttcatttatc gagaaaaacg
     1741 aggattatgg ggaaatgata gtccagcgcc caaaggacat aacgcgcaaa atggatatgg
     1801 ctgctgaaac tgcgcttgat actgccctat tgaaaagggg tctttccgcc aggataattt
     1861 ctgaggagct tggagatcgc atcatcggca aggatcctga tttcatgctt gtggtggacc
     1921 ctattgacgg ttccacaaat gccacctgtg gaataccatt tttctgcaca tctctggctt
     1981 ataccgataa aactgaaatt gccacttttg acgatatcag catggcagtt atatgcgata
     2041 ttaaggggaa tacatattat gcacagaagg gaaaaggcgc atatttgaac ggaaaagaga
     2101 tcacggggga aaaacgcggc atccggccta aaccggttgt ttctgtttat tcttatggtg
     2161 ttcctattgt acctcctggt ttgatagagc ttgaaaaatc aatcatagtc agggcgcttg
     2221 gaagcatcgc gcttgatatg tgttttgttg ctgatggtac acttgacggg atcattgata
     2281 ccaggggact tgtgagtggt tatgatatca tggcatcggc tttgatcttg aaggaatcgg
     2341 gaggaaccct tacagatctt aaaggtaata atttaacaaa taatgtccag gtaacaggtt
     2401 tatccattgc cgggacaaga aaaagcgaac ttcatgataa gatcataaga atgctggagg
     2461 tcaagtgaca acaaagctca aagcgcatat tgtttcacgg gggaaagcgc aaggttatg
//
LOCUS       DATGBN010000137         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_204487, whole genome shotgun sequence.
ACCESSION   DATGBN010000137 DATGBN010000000
VERSION     DATGBN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204487"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /gene="radA"
                     /locus_tag="VKL21_03105"
     CDS             complement(<1..715)
                     /gene="radA"
                     /locus_tag="VKL21_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HMB44790.1"
                     /translation="MVERKGLEDLPGVGPATAEKLKEAGFGSIEAIAVSSSSELASIA
                     EIGESTAQKIINAARQAADVGGFETGDIVLERRKLVGKLSFGTKAFDDLMGGGVESQA
                     ITEFYGEFGSGKTQVGHQMAVNVQLPKEEGGLEGSVIIIDTENTFRPERITQMVMGLS
                     AKKGKEYDPEEFLKNIHVAKAYNSNHQILLVDTANDLALKLKDTDRPVRLLIVDSLTA
                     HFRAEYVGRGTLADRQQMLN"
     gene            complement(896..2020)
                     /locus_tag="VKL21_03110"
     CDS             complement(896..2020)
                     /locus_tag="VKL21_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HMB44791.1"
                     /translation="MVNFEEIEEVYKRLEGKLTPDEFRSKVETKFMEMNGLCDPRTAA
                     MLVASELGMNETIKIKDLSADKGNVIFVAKVISVGDIKEFSRDNDTMGRVVNLNLADE
                     TGSIKVALWDEAADLVKIGEIKMGQAIKVKGFIKQGQRGLEVNVGRGGNVEPVEKEVP
                     VNFKPHKINEIKAGMSDLNVLGKVLDIGKVRTFARKDGKTGKVANITIGDDTGKIRVT
                     LWDSKAESPGVNVGDIVEIMNAYARENTFSNSTELQLGSGGGIARSSAIVTYKEPLTP
                     IADIGINSAYSVAGHVSGIDEIREFDRPDGTKSRVSNIYISDDTGRIKVTLWAEHAEL
                     VNEIEIGSEICIVDAFAKSGRNEEVELSAGARTSIQIIRK"
     gene            complement(2065..2808)
                     /gene="psmA"
                     /locus_tag="VKL21_03115"
     CDS             complement(2065..2808)
                     /gene="psmA"
                     /locus_tag="VKL21_03115"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HMB44792.1"
                     /translation="MMQSPQTGYDSAITVFSPDGRLFQVEYAREAVKRGTTALGIKAK
                     DGAILLVDKRATTRLVEMESIEKIFQIDDHIGVATSGLVADARVLVDFARVQAQLNKT
                     TYSEPIGIETIAKEICDFKQSYTQSGGVRPFGTSLLIAGIEGDKTRIFETDPSGALLE
                     YKATGIGSGRAEIMEFLEKKYTEDIVLDKAIILGLEALASVAESSLNELNIEIGIIEL
                     STRKFRKLIPSEVKKYVSGIPKAEKGKKK"
     gene            complement(2879..>3741)
                     /locus_tag="VKL21_03120"
     CDS             complement(2879..>3741)
                     /locus_tag="VKL21_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMB44793.1"
                     /translation="IVARAIQTEINEGRGFEGGYVHLELRHLGAEKIQEGLPGIRQIA
                     IDFANIDPIEKPIPVQPGQHYSMGGVASNKNCETSILGFYVCGECSCISVHGANRLGG
                     NSLLETLVFGKISGENAEKFANSSTFGEKELIERAIEEEKKKISVLLERKVGEEFFKI
                     RDELKTILDEKAGIFRNEEDLTRALEKIRELKTRYGNVYVRNKGTIFNQELVNVIELE
                     GMLDVAHAICSGAKARKESRGSHFRLDYPVRDDTNWLKHTIITFAPDGARIDYKSVNL
                     NMFAPKPREY"
BASE COUNT          847 a          988 c          710 g         1196 t
ORIGIN      
        1 tattgagcat ctgctgcctg tctgccagcg ttccgcgtcc cacatattcc gccctgaaat
       61 gggctgttaa ggaatctact atcaggaggc gcaccggcct gtcggtatct ttcagtttca
      121 aagcgagatc attcgcagtg tccaccagca gtatctggtg gtttgaatta taggcttttg
      181 caacatggat gtttttcagg aactcctccg ggtcatattc cttccccttt tttgctgata
      241 atcccatcac catctgggtg atcctttcag gtctgaaggt attctctgtg tctattatta
      301 ttaccgatcc ttcaagtccc ccttcttcct ttggaagctg cacattcact gccatctggt
      361 ggccaacctg ggtctttccc gatccgaatt cgccgtagaa ttccgttatc gcctgggact
      421 caacccctcc gcccataagg tcgtcaaagg cctttgtgcc aaagctgagc tttcccacaa
      481 gtttcctgcg ttcaagaaca atgtcgcctg tttcaaatcc cccaacgtct gcagcctggc
      541 gcgccgcatt aatgatcttc tgtgctgttg actcccctat ttccgcgatg cttgccagtt
      601 cagatgagga tgatacggct atggcctcta tcgagccaaa acctgcttct ttcaatttct
      661 ctgctgttgc cggacctacg cccggtaaat cttccagacc ttttctttcg accatgttgt
      721 tatctccagt gcacgtttta tctttttttg gtgcacaatt gttctttatc ttggcgagtg
      781 ttaagataaa ccttatgaga gcggggtgaa agtaatattt atgatgattt tatccaatat
      841 ttccgctatg ataatgaacc ccattagttc cactggagaa cagcatcgtg caaactcatt
      901 ttcgaataat ctgaatgctc gtcctggccc cggcgctgag ttccacttct tcattcctac
      961 ctgattttgc aaaagcgtcc acaatgcaga tttcgcttcc aatctcaatt tcattgacaa
     1021 gctctgcgtg ttcagcccag agcgtcactt ttatgcgccc cgtgtcatct gatatgtaaa
     1081 tgtttgatac cctgcttttt gtgccatcag gcctgtcgaa ttcccgtatc tcatcaattc
     1141 cggaaacatg ccctgccaca ctgtaagcag agttaatacc tatatcggct attggtgtga
     1201 gcggttcctt ataggtaaca atggcgctgc ttctggcaat tcccccgccg gaacccagct
     1261 gcagttccgt ggagttgctg aatgtatttt cacgcgcgta ggcattcatg atctccacga
     1321 tgtctccgac attgacaccc ggagattcgg cttttgagtc ccataacgtg acccggatct
     1381 tccctgtatc atcccctatt gtaatatttg cgactttacc cgtctttcca tcttttcgcg
     1441 cgaatgtgcg cacttttccg atatcgagaa ccttgccaag aacatttaga tcgctcatcc
     1501 ccgctttgat ctcattgatc ttatggggtt taaaattcac cggaacttct ttttctacag
     1561 gctcaacatt cccgcccctt cccacattca cttcgagtcc gcgctgcccc tgcttgatga
     1621 atcctttaac tttaatagcc tgccccattt ttatctcgcc tatcttcacc aggtcagcag
     1681 cttcgtccca taatgcaacc ttgatagagc ccgtttcatc tgcaagattc agatttacga
     1741 cgcggcccat agtatcatta tcacgtgaaa actccttgat atccccaacg gatatcacct
     1801 ttgcaacaaa aataacattg cccttatcgg cactcagatc cttgatctta atggtctcat
     1861 tcattcccag ttcgcttgca acaagcatag cggcagtcct tggatcgcaa agaccattca
     1921 tttccatgaa tttcgtctca acctttgacc tgaattcatc aggtgtaagc ttaccttcaa
     1981 gtcttttata gacctcttca atctcttcga aattcaccat gctatgctaa gaaggatagt
     2041 ttggataaaa aacttatggt caggttattt tttctttccc ttctcagctt tggggatccc
     2101 tgacacatat tttttcactt ccgaagggat cagcttccgg aattttcgcg ttgagagttc
     2161 tattattcca atttcaatat ttaattcatt cagggagctt tccgctacgc tcgcaagcgc
     2221 ttcaagtccg agaataatag ctttatccag aacaatatct tcagtatatt tcttctccag
     2281 gaattccatt atttcagctc ttcctgagcc aattccggtg gctttgtatt caagcaatgc
     2341 cccgctgggg tcggtttcga aaatgcgcgt tttatctcct tcgatgccgg caataagaag
     2401 agaggtcccg aatggtctta ctccgcctga ttgggtgtag gactgcttga aatcgcagat
     2461 ctctttggcg atggtctcaa tgccaatagg ctcactatat gtggtcttgt taagctgggc
     2521 ctgtactcgg gcgaagtcta caagaacgcg ggcatccgct accaatcccg atgttgccac
     2581 accgatatga tcatcaatct ggaaaatttt ctcaatagat tccatctcaa caagtcttgt
     2641 ggtggcgcgc ttatctacca ataagattgc gccgtctttt gctttaattc ccaatgctgt
     2701 tgttcctctt ttcacagctt cccgtgcgta ttctacctgg aacagcctcc catcagggct
     2761 gaatacggtt atagcactat catatcctgt ttgtggtgac tgcatcatat tgtctcctgg
     2821 tttttatgtt tgaaggtcgc agagtatata tctatttcca tcctgtaaca aaaaaccctc
     2881 aatattctct tggtttgggc gcaaacatat taagatttac cgatttataa tcaattcgtg
     2941 cgccatctgg tgcaaatgta attatagtat gttttaacca attagtatca tcccttaccg
     3001 ggtaatcgag acgaaagtga gaaccacggc tttccttgcg ggcttttgct cctgaacata
     3061 tcgcatgcgc aacatccagc attccctcaa gctcaatcac gtttacaagt tcctggttga
     3121 aaatagttcc cttatttcgg acatatacat tcccataacg tgttttaagt tccctgatct
     3181 tttcaagagc tcttgttaaa tcctcctcgt tccggaatat tcccgctttt tcatcaagaa
     3241 tggttttgag ctcatctcgt atcttgaaga attcctcccc aacttttcgt tctaataata
     3301 ctgatatctt tttcttttcc tcctctattg ctctttcaat caattctttc tcaccgaacg
     3361 tactcgaatt tgcgaatttc tcagcatttt ctcctgatat cttcccgaaa acaagagttt
     3421 cgaggaggga gttccctcca agcctgttag ctccatggac actgatgcag gaacattctc
     3481 cgcatacata gaatcccaaa atcgatgttt cgcagttctt atttgaggcg acgcctccca
     3541 tggaataatg ctgtccgggc tggaccggaa ttggtttttc aatcgggtcg atattggcaa
     3601 aatcgatagc tatctgcctg atccccggca gaccctcctg gattttttcc gccccaagat
     3661 gtctgagttc aaggtgaaca tatccaccct caaatcctct gccttcattt atctcggttt
     3721 ggatagcgcg cgccacgatg t
//
LOCUS       DATGBN010000138         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_282204, whole genome shotgun sequence.
ACCESSION   DATGBN010000138 DATGBN010000000
VERSION     DATGBN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282204"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VKL21_03125"
     CDS             <1..588
                     /locus_tag="VKL21_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HMB44794.1"
                     /translation="DVAYTTRIVTRKGSERIAECACLFARKRKERLTIVHKANVLKSD
                     SFFRDVCTGVAKKNNISYNEMLVDAMAYDMILNPGKYDVIVTTNLFGDILSDEAAAIA
                     GGLGLCPSANIGDRYALFEPIHGSAPDIAGKGIANPIAAILSVRMMLDWAGNTEEARS
                     IDKAVDSVLASHISTPDLGGGYSTHRVGEAIARLV"
     gene            818..2674
                     /locus_tag="VKL21_03130"
     CDS             818..2674
                     /locus_tag="VKL21_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMB44795.1"
                     /translation="MSTSKNDKPDLFLQSVIDGVPDCILVIGPDYEVKLMNRAAREFS
                     LKFSGEFQQCYKIFHRREKPCMSAGHNCPLEEVRKTGQPDRVVHEHYNSNGEKRSVEI
                     LSSPLYGADGTFQGIIQCSRDITMSTQAEKDLTATIAAVEDEKNKSEAIIAALGDGII
                     IQDTDYKIIYQNQVQNDLYGNRMGELCYRAYEGRDKICEDCPVEKTFRDGKIHMSERR
                     VPTDKGVSYFELTSSPLKDSTGKITAGIKVVREITQRKHDEEELRESERFMDSIFASI
                     QDGIGIIDKDMNIIRVNKTGESWYSHAMPLVGKKCYEAHHNRKEQCEFCPARKTLVTG
                     NSAYKIVPKHGPGGKEVGWLEIYSYPLKDTTTGQMKGVIEYVRDITERKHIEEAIKVY
                     NMELKESNRIKELFADIMHHDLLNPLNVAHCYVELFLEDETNHQKKSYLESIKRNLVK
                     GMELIENATKFSKLESMKKIEFENLDLKMIVGEAIENLTPLAVRAGMSIENNLKGRMP
                     ARANKIIEDIFINLISNSIKYAPEGKRIVVDGKDGGTFWIIRVIDFGAGIKDADKKMI
                     FERFHREEKKGVKGSGLGLAIAGKIVELHKGRIWVEDNPEGGALFAVEIPKS"
     gene            complement(2699..>3334)
                     /locus_tag="VKL21_03135"
     CDS             complement(2699..>3334)
                     /locus_tag="VKL21_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMB44796.1"
                     /translation="VGLGFFFGASKINAPFIGYIISWWIISPLLAWLLAYLMGKYLYT
                     HILIWLADHHESDASIRKSLNILLTLSGCYVAYSAGANNAANAVGPFVGAGIIGSIPG
                     AIFGGLAIGLGALVMGGRVLDTVGKEITELCVIRATFVEFTSAFIVHIASIKGIPVSL
                     GEIVAAGIIGIGCANSGMHLVKGEVVKKILIAWVVSPLLAGILAFGLMKLI"
BASE COUNT         1103 a          645 c          773 g          813 t
ORIGIN      
        1 gatgtcgcat acactaccag gatcgtcaca cggaagggaa gtgagagaat agcagaatgt
       61 gcctgccttt ttgcaagaaa aagaaaggaa cgcctgacga tcgttcacaa agcgaatgta
      121 ttgaaatccg actctttttt ccgtgatgta tgtaccggtg ttgccaaaaa aaacaatatt
      181 tcatataatg aaatgctcgt tgatgccatg gcgtatgata tgatcctgaa tccaggaaaa
      241 tatgatgtga tagtcaccac gaatcttttc ggagatattc tcagtgacga agccgcagca
      301 attgccggcg gcctgggtct atgtcccagc gccaacatcg gagaccgtta tgcacttttt
      361 gaacctatcc acgggagcgc tccggatatc gcaggcaaag ggattgccaa tccgatagct
      421 gccatactta gcgtcaggat gatgcttgat tgggcaggga atacagaaga ggcaaggtca
      481 atagataaag ctgttgacag tgttcttgct tcgcatatca gtacgcctga tcttggaggc
      541 ggttattcca cccatcgggt gggtgaagcc atcgcccgcc tggtctaaag ctttaagact
      601 attattatct cggtgataat aatagaaagt attattaggc aaacggaaaa ttaatgcttt
      661 aatataaggg caaataaggt gttggtacct taatgagtta cctgaacaat ggatagggaa
      721 ctgccaggat gaagaatcct caggcagcat atattctctg catataactg taaaaaaatg
      781 aagcgcaaat acttcataaa aaacaaaact aaaaaacatg agcacctcta aaaatgataa
      841 accggactta tttctgcaat cggtaattga tggtgtgccg gattgcatcc ttgttatcgg
      901 cccggactat gaagtgaaat tgatgaacag ggccgcacgc gagttttcgc ttaaattttc
      961 cggtgaattt caacaatgct acaagatttt ccatcggaga gaaaaaccgt gtatgtctgc
     1021 gggacataat tgtcctctgg aagaagtgcg aaagaccggt caaccggaca gggttgtgca
     1081 cgaacattat aactccaatg gagagaagcg atccgttgag atcttgtctt caccattgta
     1141 tggagcggat gggactttcc agggaataat ccagtgttct cgcgacatca ctatgagtac
     1201 acaagcggaa aaagacctga cggcaacaat tgcagcggtt gaggatgaaa agaacaaatc
     1261 agaggctatc attgcggcct taggcgatgg tataataatt caggatactg attataaaat
     1321 aatataccag aaccaggttc aaaatgattt atatgggaac aggatgggtg aactttgtta
     1381 cagggcttat gaaggtagag ataaaatttg cgaagattgc ccggtcgaga agactttcag
     1441 ggatggaaaa atccacatgt ctgaaagaag agttcccaca gataagggag tttcatattt
     1501 tgaacttact tcatcgccgc taaaggattc taccgggaaa atcacagcag ggatcaaggt
     1561 tgtaagggaa attacacaac gaaaacatga tgaggaagag cttcgtgaaa gcgaacgatt
     1621 catggatagc atctttgcaa gtatccagga cggtataggg ataattgaca aagatatgaa
     1681 cattatcagg gtcaataaga caggcgaaag ctggtattcg catgctatgc cgcttgttgg
     1741 gaagaaatgc tatgaagcac atcacaacag aaaagagcaa tgtgagtttt gccctgcccg
     1801 gaaaacactt gttactggta attcggccta taaaatagtc cccaaacacg gacccggggg
     1861 aaaagaagtg ggatggcttg aaatttacag ttatcctctt aaagatacaa caacggggca
     1921 gatgaaaggt gtaatcgaat atgtgcgcga tataacagaa cgcaaacata ttgaagaagc
     1981 aattaaggta tataatatgg aacttaagga atccaatcgc ataaaggaac tcttcgccga
     2041 catcatgcat catgacctct taaatcctct taatgtggca cactgttatg ttgaactttt
     2101 cctggaggat gaaacaaatc accagaaaaa atcatacctt gaatccatta aaagaaatct
     2161 tgtaaaggga atggaactga tcgaaaatgc cacgaagttt tcaaaactcg aaagtatgaa
     2221 aaaaattgaa tttgagaacc tagatttgaa aatgatagtc ggggaggcta ttgaaaatct
     2281 cacacctctg gcggtaagag ccgggatgag tattgagaat aatcttaaag gcaggatgcc
     2341 tgcaagggcc aataaaataa tcgaagatat ttttataaac cttatttcca attccataaa
     2401 gtatgcccct gaaggaaaaa ggatagttgt ggatggcaaa gatggcggaa ccttctggat
     2461 tatcagggtt attgacttcg gggcgggaat taaagatgca gacaagaaaa tgattttcga
     2521 aagattccac agggaagaaa agaaaggagt gaagggttca ggattgggcc tggccatagc
     2581 gggaaaaata gtggaacttc acaaaggcag gatatgggtt gaggataatc cggaaggtgg
     2641 agctcttttt gcagtggaga tacctaaatc atagatcaac ttcattaatc gaataatctt
     2701 atatcaactt cattaatccg aatgcaagta ttcctgcaag aaggggtgaa actacccatg
     2761 cgatcaatat ttttttaaca acttcccctt ttactaaatg cattccgcta ttagcgcatc
     2821 cgataccgat tatgccggcg gcgacaattt ctcccagtga tacaggtatt cctttgatcg
     2881 atgcaatatg gactatgaag gctgaggtaa actccacgaa tgtagccctg ataacacaga
     2941 gttcggtgat ctcctttcca acggtgtcaa gcaccctacc gcccatgaca agagcaccaa
     3001 gaccaatggc aagacctccg aaaatcgctc cgggaattga cccgataatt cccgctccca
     3061 caaaaggccc aacggcattt gcagcattgt ttgcaccggc agaatatgcc acataacaac
     3121 cagataaagt caggagaata ttaagggact tccttattga agcatcggat tcatggtgat
     3181 cggcaagcca tatcaatatg tgcgtgtaaa gatattttcc cattaaatat gcaagcagcc
     3241 atgccagtaa gggactgatg atccaccagc ttataatata accgataaaa ggcgcattta
     3301 tttttgaggc cccaaagaaa aaacccagac ccac
//
LOCUS       DATGBN010000139         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_10441, whole genome shotgun sequence.
ACCESSION   DATGBN010000139 DATGBN010000000
VERSION     DATGBN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10441"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(696..>1946)
                     /locus_tag="VKL21_03140"
     CDS             complement(696..>1946)
                     /locus_tag="VKL21_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44797.1"
                     /translation="LFVVLAFISFWGITFPAFIQLTQGLKVGVASDTKNFFNIWSYPF
                     TIILLLALGFCLNYNETKKEEQKRILFIVIGLSIITMFARTESFYVLDHSSPFWVREP
                     FFYKLIGNISVVSLFPSMIYATWAIIKRSFDYLRIESIRPKIKGIGIAIVHFGFIFIL
                     FGTVISSNFTQKIEGINIPLSAKGENVDIGHGYGIKIVDFTTSSLTGTAPADGLKISE
                     LYTSPDNYISNNIKVSGKVTNIENVQSPPPVTYTTYMQIDDGTESLWVATQANEPLNY
                     QEGMELTIGGFLMTNFRSNSTNTTYPLILFSTPEQIIQEAATSGKYQVQSVRLEVYQD
                     NKKIGSGVAEYLNSQSGSGTFPLVDSSITGTDVYVIFQGIGGGTIPLSLRIIPAVNFA
                     WIGIVLFAIGIILIMAVKSRTKGR"
BASE COUNT          571 a          415 c          357 g          603 t
ORIGIN      
        1 tccatgtacc gtttccattg gaaaccgtca cggcttgtaa gatactgttt ccacccgata
       61 gatatagtag cttttttttt tcacattggg tatgtgacgc tcactcatca acttagaaac
      121 ctgttctggg gcccattcgg ctgcctgaca gaaattgaat gaaactcctg tttcttcagg
      181 aaaagcattt tccattaagt tataagttta aaagcctgag acttaaaagg ccaatgctcc
      241 cttgaactca tagagttatg gaacttggag aatatcggca acatccaaga aacgataatg
      301 tcagatatta taaaggtctt caagacattc atgtttgtgt tcgtatcatg tccctgggta
      361 aagctccaga atatctaaaa cttcagccca taaagacaag cggttgtaaa ggaatagttc
      421 atagttttat tttgctccat tctagtgcta aaaaatatta ataaaaacca acgaatttga
      481 ttttgagttc gctgttcaag catacgagta tgacctcaaa actaacattg tacttatgag
      541 gtgttctgaa agcctcacaa tcattaccta tgatacctgt ctgtgtgatc ttttcagatt
      601 gtattgtccc atgctgagga ggtaaatacc tccccaggaa aaagcgaagg ttcaataacc
      661 cactgatatg ctacaatatc ctgtggaatt atttcttatc tgcctttcgt tcttgatttc
      721 actgccatta tcaggattat cccaatcgcg aagagtacga tgccgatcca tgcaaaattc
      781 accgcaggta tgatcctcag cgaaagagga atcgttccac caccaatacc ctggaaaata
      841 acataaacat ccgttcctgt aatgctgcta tctacaagcg ggaatgttcc gctcccgctc
      901 tgtgaattaa ggtattctgc gactccactt ccaatttttt tgttatcctg gtatacttca
      961 agccggacac tctgtacctg gtatttgcct gaagtagctg cctcctggat tatttgttcc
     1021 ggagttgaga ataaaataag agggtatgtc gtatttgtag agttactcct gaaatttgtc
     1081 atcaggaagc cacctattgt aagttccatc ccttcctgat aattcaaggg ttcattcgcc
     1141 tgcgttgcga cccataggct ctctgtcccg tcatctatct gcatataggt ggtataagta
     1201 acagggggtg gggattggac attttctata tttgttactt tgccgctgac tttaatatta
     1261 ttacttatat aattatcagg acttgtgtat aattccgata tctttaatcc atccgccggc
     1321 gcagtgccgg taaggctgct cgttgtaaaa tcaactatct taataccata accatgcccg
     1381 atatctacat tttctccttt tgccgaaaga ggtatattga tcccctcaat tttctgggtg
     1441 aaatttgagc taattactgt tccaaaaaga atgaatataa agccaaagtg aacgatcgct
     1501 atcccgatcc cttttatctt tggtcttata ctttcgatcc gcaaataatc aaaactgcgt
     1561 tttattattg cccatgttgc atatatcatt gatgggaaaa gagatacaac cgagatattg
     1621 ccgattaatt tatagaaaaa tggctccctt acccagaagg gactgctatg atcaagtaca
     1681 taaaagctct cagttctagc gaacattgta attattgaca ggccaattac tatgaacaat
     1741 atccttttct gttcttcctt tttcgtttca ttataattca aacagaaacc aagcgcaagc
     1801 aacaggatta tcgtgaaagg ataactccag atgttaaaga aattctttgt atctgaggct
     1861 actcccactt taagaccctg cgtaagctgg ataaaagccg ggaaagtaat accccagaaa
     1921 gatatgaatg ccagcaccac gaacaa
//
LOCUS       DATGBN010000140         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_437717, whole genome shotgun sequence.
ACCESSION   DATGBN010000140 DATGBN010000000
VERSION     DATGBN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437717"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VKL21_03145"
     CDS             complement(<1..652)
                     /locus_tag="VKL21_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMB44798.1"
                     /translation="MELDESEFYNQLLDYNNILYLCHRNADPDALGSALALKEAIGGT
                     IGVVDGCDRVASQLAAQLNIEYVLAPKGDYDFVVVVDTSTPAQLNGFPLKSYAVIDHH
                     ATSSLSENSAFFLHRNKSSTAEIVLDVLKKMGAPIMRRTAFALISGIITDTGNFRHAS
                     SDSFKAVAELMDLSGIEYNEVIDLLSSVPQDVSMRIAFLKAAQRLIIERVDDWIIVS"
     gene            complement(655..1011)
                     /locus_tag="VKL21_03150"
     CDS             complement(655..1011)
                     /locus_tag="VKL21_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HMB44799.1"
                     /translation="MSSELPPQIQNQLAQLQQIQQQAQALAAQKNQIEINLKETELAL
                     GELEKLDDTAVVYRAIGDLLIKTEREKTTNALIEKKDTLSLRTETITRQEERIQKRFQ
                     QLQEQLKQAIGNPVAK"
     gene            complement(1050..1304)
                     /locus_tag="VKL21_03155"
     CDS             complement(1050..1304)
                     /locus_tag="VKL21_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HMB44800.1"
                     /translation="MLVLAEFEFDAGKDARIIYEALLPELDQDYQRTRTTLGLKSEVL
                     VLKVEANDTVSMRAALNGWLRLIKITYEMCCFTQDTQVIN"
     gene            1377..2432
                     /locus_tag="VKL21_03160"
     CDS             1377..2432
                     /locus_tag="VKL21_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMB44801.1"
                     /translation="MIKHPMDPWIKIEIITISALIVLSAFFSISETALLSVNKIRIRH
                     LAETGNKQAKVLIRLLENPELFIAAILIGNNIVNISASVLATDAALAYFGQSGIAVAT
                     GVMTLFILVFGEVFPKTLASRNAEYIALRVSGPITTTIRFLRPIVWFITRIVNSMILL
                     LGGKERVKHPFVTEEMIELMLKVGEKEGTIEKHEREIISRVFDFTDEKAHGVMTPREK
                     IVSIEESQTLDIALDLINQSGHSRIPVYRNEFDNIMGMIYAKDFLKFRDHDLPRTKVF
                     QILRPLQVVRAGKEISSILKELQQKKMSISIVVDNNMRVIGLVSIEDILEELVGEIFD
                     EYDIETKPVLTGNVPDR"
     gene            2489..>2956
                     /locus_tag="VKL21_03165"
     CDS             2489..>2956
                     /locus_tag="VKL21_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMB44802.1"
                     /translation="MPKRSDIKKVLLIGSGPIMIGQGAEFDFSGSQACRSLREEGIKV
                     VLVNSNPATIMTDTDMADSVYIEPLEPEIVAKIIEKERPDGIIAGLGGQTGLNLTSEL
                     AEMGALEKFGVELLGTSLQAIKDSEDRDLFKRKMISIGEKVPRSKAVNTLDEAL"
BASE COUNT          863 a          597 c          660 g          836 t
ORIGIN      
        1 atgaaacaat tatccagtca tccacacgtt cgatgatcaa acgctgggca gccttcaaaa
       61 aagcaattct cattgagacg tcctgaggaa ccgaagaaag aagatcaata acttcgttgt
      121 attctatgcc gcttaaatcc ataagttcag ctacggcttt gaatgaatcc gaagaagcat
      181 gcctgaagtt gccggtatcg gtaattatac ctgatataag tgcaaaggct gtgcgtctca
      241 ttatgggtgc ccccattttc ttcaagacat caagaactat ctcggcagtt gaactcttat
      301 tgcgatgaag gaaaaaagct gaattctcgg aaaggcttga ggtggcatga tggtcaataa
      361 cagcataaga ctttagcgga aaaccattca gctgtgcagg cgttgatgtg tccacaacga
      421 ccacaaagtc ataatctccc tttggcgcaa gaacatattc tatattcaac tgggctgcga
      481 gctgggatgc aaccctgtcg catccatcaa caaccccaat tgtaccccca attgcttctt
      541 ttaacgctaa tgcactaccc agggcatcag gatctgcgtt cctgtgacat aaataaagaa
      601 tattgttgta atcgaggagt tgattataga attccgattc gtctaactcc ataattactt
      661 ggcgaccgga ttaccgattg cctgcttgag ctgctcctgt aattgctgga atcttttctg
      721 gatgcgttct tcctgtcggg taatagtttc tgtacgcagg ctaagggtat cttttttctc
      781 aatgagggca tttgttgtct tttcacgttc tgttttgatg agcaaatctc cgatcgccct
      841 gtagaccaca gcagtatcat caagtttttc aagttctccc agagccagtt cagtttcttt
      901 taaattgatc tcgatctggt ttttctgggc tgccagggcc tgtgcctgtt gctgtatctg
      961 ctgcaattgt gcaagctgat tttgaatttg cgggggtaat tctgaactca tttaattcac
     1021 ctgtaaatct gatgtagtat tgtacatctt taatttataa cttgtgtatc ttgcgtgaaa
     1081 caacacatct cgtatgtgat ttttatcagc cgaagccaac cgttcaaggc agccctcatc
     1141 gaaactgtgt catttgcctc aacttttaag accagtactt cacttttaag tccaagagtt
     1201 gtcctggtgc gctggtaatc ctggtcaagt tccgggagta atgcttcata gattatgcgg
     1261 gcatccttcc cggcatcgaa ttcaaattct gcgagaacaa gcatcaaacg tgataagttt
     1321 atgttgtata tcaaggtttt cagaaaaatt tttaagctgg attgatatta tatgacttga
     1381 tcaaacatcc gatggaccca tggataaaaa ttgaaataat aacaatttct gcattaatcg
     1441 ttctttccgc gtttttttca atttcagaaa cagctctcct gtcagtcaat aagattcgga
     1501 taagacatct tgcagagaca gggaataaac aggcgaaagt attgataaga ttacttgaaa
     1561 atccggagct tttcatcgca gctatcctga taggtaataa tattgtaaat atcagcgcct
     1621 ctgtactggc aacggatgcg gcgcttgctt attttggcca gtcggggata gctgtcgcaa
     1681 caggtgtgat gacgctgttc attcttgttt ttggtgaagt tttccctaaa acccttgctt
     1741 caaggaatgc tgaatatatc gcccttcgtg tttcagggcc aataacaacc actatccgtt
     1801 ttcttcggcc tattgtctgg tttatcacca ggatagtgaa ttccatgatc ttactcctgg
     1861 gcggaaagga acgtgtaaag catcctttcg tcacggaaga gatgatcgaa ttaatgctca
     1921 aggtagggga aaaggagggg actatcgaga agcacgaacg agagataata agcagggtgt
     1981 ttgatttcac tgatgaaaaa gcgcatggag taatgacgcc cagggaaaag atcgtatcca
     2041 tagaagaatc ccagacactt gatattgcac ttgatctgat caatcagtcg ggtcattcca
     2101 gaataccggt atacaggaat gagttcgata atatcatggg tatgatctat gccaaagatt
     2161 ttttgaagtt cagagaccat gacctcccaa gaacaaaagt tttccagatt ttgcgtccat
     2221 tgcaggttgt cagggcagga aaagagatat cttcaattct taaggaactc cagcaaaaga
     2281 aaatgagtat ttcaatagtt gtggataata atatgagggt cataggatta gtttcaattg
     2341 aggatatcct ggaagagctt gtgggtgaga tctttgatga atatgatatt gaaaccaaac
     2401 cggtattgac cggaaatgta cccgatagat gaacaatgga tacaattaat aacacatctt
     2461 accattaagc ctgaaaaaca gaggtaaaat gccaaaaaga tccgacatca agaaagtgct
     2521 gcttataggt tcaggcccta taatgatagg acagggggcg gaatttgatt tttcaggcag
     2581 ccaggcatgc cgctcccttc gggaagaggg aataaaagtt gtcctggtca acagcaatcc
     2641 agctaccatt atgacagata ccgacatggc agactcggtt tacatagagc ctcttgagcc
     2701 cgaaatcgtg gcaaagataa tagaaaaaga aagacctgat ggcattattg ccggtcttgg
     2761 aggacagacc ggtcttaatc ttacatctga acttgcagaa atgggcgcgc tggaaaaatt
     2821 cggcgtggag cttctgggga catccttgca ggcgatcaaa gactcggaag acagggatct
     2881 ctttaagaga aaaatgataa gcattggcga aaaagtcccg cgctcaaaag ctgtgaacac
     2941 acttgatgaa gctcta
//
LOCUS       DATGBN010000141         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_243779, whole genome shotgun sequence.
ACCESSION   DATGBN010000141 DATGBN010000000
VERSION     DATGBN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243779"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="gatE"
                     /locus_tag="VKL21_03170"
     CDS             complement(<1..730)
                     /gene="gatE"
                     /locus_tag="VKL21_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HMB44803.1"
                     /translation="MNRDYRALGLKSGLEIHQQLDTKEKLFCGCPTLLRDTKESNFEF
                     FRYLRPTQSEMGIVDKAALEEIKLIKKFIYKAYDTTCLVENDEEPPRELNPEAVDNAL
                     TIARMMNMKIVDELYTMRKIVIDGSNTSGFQRTGLVATHGYIDSSAGRVGIDVLCLEE
                     EAAQKVEDKGDSVIYSLDRLGIPLVEIGTAPDIVSPAHAREVAEQLGMILRSTGKVKR
                     GLGTIRQDVNISIAQGSRVEIKGVQ"
     gene            819..1625
                     /locus_tag="VKL21_03175"
     CDS             819..1625
                     /locus_tag="VKL21_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMB44804.1"
                     /translation="MTLVKKAKECADHIKNHTSALVVSHIDADGLTSAAIMRTALERS
                     GIDSQVIFLKQLDHKNLQEIADLNLQLVIFTDLGSGMLDAIKSLKVNAVIADHHQPQG
                     SYEYHLNPHIFGFNGGIEVSGSGMTYFIAREMGDNGDLAALAIVGAVGDLQHSKHGQL
                     IGLNRNILDEGVKNKVIKYEKDLMLFGRQTRPVFKLLQYSTDVYFPGLTGNEEASIEF
                     LKRIGIRQQGEKWRRWIDLDTSEKQKIVSALIQFGLSSGIPGFKVERLVG"
     gene            1743..2183
                     /locus_tag="VKL21_03180"
     CDS             1743..2183
                     /locus_tag="VKL21_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HMB44805.1"
                     /translation="MGDRGKSFDEACVLLSEHRKNLVEGLNFVKEKGITELHNLQYFD
                     SGNKIRETIVGIVAGMSASFVNNRNLPIIAFADSEGGIKVSSRGNQDLLRKGLNLGAA
                     ISQAAKAVGGNGGGHDIAAGAFIPRESKNEFLKILDMKIGSQIN"
     gene            complement(2226..3188)
                     /locus_tag="VKL21_03185"
     CDS             complement(2226..3188)
                     /locus_tag="VKL21_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HMB44806.1"
                     /translation="MILGIDIGGANTKIASDDGKIVELHYIPLWKNTMLPGMLLEIAQ
                     RLKPEKVGVVITGELADCFPDKDAGLSYIIDAVNNVFENAFFLDNNGDLTTEKKRSIA
                     AANWMASSLLTGKEYGDCIFVDIGSTTTDIIPIKNGKPLASKTDFIRLKKGELVYSGA
                     LRTNIAAILKRVKFGNSESRISSELFAITADAYIVLGLIEQENYTCDTPDGAGKTLID
                     AKRRIARIVCADLNEIRDDEVFSIARQVMDAQVSDIRAAIHEISQRYSIRRIVACGLG
                     EFLAKNAVIEDGLEMILISEKYGAQISKVFPAYAAARLLKDENI"
     gene            complement(3265..>3765)
                     /locus_tag="VKL21_03190"
     CDS             complement(3265..>3765)
                     /locus_tag="VKL21_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB family protein"
                     /protein_id="HMB44807.1"
                     /translation="WLKAIFIAVILMILAMLVLLVFGGYPLEKFLTAMLYLFLAQGIL
                     SALGVLVVRGHPLSALTAFGLAWFGFLHPFLAIGWLAGFVEAHFRPPTTEDFRTIMKS
                     ETMTELMQNKLSRILLVALMANLGSMAGTFVAIPIMVHYLGITNPLDILKMALETGFN
                     TVKSFF"
BASE COUNT         1113 a          804 c          788 g         1060 t
ORIGIN      
        1 cctggactcc ttttatttca actcttgagc cctgtgctat tgagatattg acatcctgcc
       61 ttattgtgcc aaggccgcgc tttacctttc ctgttgagcg caatatcatc cccagttgtt
      121 ctgcaacttc ccttgcatgg gcaggggaaa ctatatcggg cgcagtccct atttccacaa
      181 gcggaatacc gagccggtca agagaataaa taacagaatc ccccttatct tccactttct
      241 gcgcagcttc ttcttccagg cagagcacat caatacccac gcgccctgcg cttgaatcta
      301 tgtatccgtg ggtagcgaca aggcctgtcc tctggaaacc ggaagtattc gagccgtcga
      361 tcacgatttt tcgcatggtg tataattcat ccacgatctt catgttcatc atgcgggcta
      421 tcgtgagagc attatccaca gcttccgggt ttaattcacg cggcggctcc tcatcgttct
      481 caacgaggca tgtggtgtcg tatgctttat aaataaattt ttttatgagc tttatttctt
      541 caagcgctgc tttatctacg attcccatct cgctctgagt aggacgaagg tatctgaaaa
      601 attcgaaatt tgattctttt gtatcacgaa gaagggtggg acaaccgcag aatagttttt
      661 cttttgtgtc aagctgctgg tgtatttcaa gcccggactt taatccgagc gccctgtaat
      721 cgcgattcat aaagcttcct gatagaatgc aatctataaa aaaattgtta tcggtctgtc
      781 aaaggttata taaataattg tggctacctg aactatgaat gacgctcgtt aaaaaagcaa
      841 aggaatgtgc agatcatatc aagaatcata cttctgctct cgtggtttcg catatcgatg
      901 ctgatggcct gacttcagca gcgataatga ggacagctct tgaacgcagt gggatcgatt
      961 cccaggtcat ttttttaaaa caacttgacc ataaaaacct ccaggaaata gccgacctga
     1021 atcttcaact tgttattttt acagaccttg gcagcggaat gctggatgcg ataaaatcgc
     1081 taaaggtcaa cgcggtaatt gcagaccatc accagcccca gggcagctat gaatatcatc
     1141 ttaatcccca tattttcggt tttaacggag gaattgaagt aagcggctcc ggaatgacat
     1201 attttatcgc gagggaaatg ggtgataatg gagaccttgc agctcttgct attgtaggag
     1261 ctgtagggga tcttcaacat tcaaagcacg ggcaattaat aggtttgaac aggaacatac
     1321 ttgatgaagg agtaaagaat aaagttatca aatatgagaa agatctcatg ttattcggaa
     1381 ggcagacacg tcctgtattt aagctcctgc aatattcaac tgatgtctat tttccaggcc
     1441 tgacaggaaa tgaagaagca agcatagaat tccttaaacg aataggaata agacaacaag
     1501 gagagaaatg gagacgatgg atagatcttg atacctctga aaaacaaaag atcgtatcag
     1561 ctcttataca atttggcctt tccagcggga ttcccggttt taaggtagaa agactcgttg
     1621 gttaggtata cacattatta tgcgaacggg aaggtacaga ggtcagggac gcctcggaat
     1681 tttcaactct ccttaattct actgcccgtt atgaccatgc agatatcggg ctttcggtat
     1741 gtatgggcga caggggaaag agtttcgatg aagcatgcgt cctacttagc gaacatagaa
     1801 aaaatcttgt ggaaggatta aatttcgtaa aagaaaaagg tattacggaa ttgcacaacc
     1861 tccaatattt tgattccgga aataaaatac gtgaaacaat tgtgggcatc gtagctggaa
     1921 tgagcgcgtc gtttgtcaat aacaggaatc ttcccataat cgcttttgca gattctgaag
     1981 gcggcattaa ggtttcatca cgggggaacc aggaccttct caggaaaggg ctgaaccttg
     2041 gcgctgcaat aagccaggcg gcaaaagcag tgggcggaaa tggaggcggg catgatattg
     2101 ctgcaggcgc attcattccg agagaatcaa aaaatgaatt cctgaaaatt cttgatatga
     2161 aaataggctc acagataaat taaaaaagaa aaatacttcc gaaattgaaa gggtgaaatc
     2221 ttcctttaga tattttcatc tttcaataac cgtgcagcag cataagccgg aaatactttt
     2281 gatatttggg ccccatattt ttcagagatc aatatcatct caagaccatc ttcaattact
     2341 gcattttttg ccaggaattc tccaaggccg catgccacaa tccttctaat actatatctt
     2401 tgcgatattt catgaattgc agccctgata tcactgacct gcgcatccat aacctgtctt
     2461 gcgatcgaaa aaacctcatc gtctcttatt tcattcaaat ctgcgcatac tatccgggca
     2521 attcttcttt ttgcgtcaat taatgttttc ccggcgccat ccggtgtgtc gcatgtataa
     2581 ttctcctgtt ctatcaatcc aagaacaatg tatgcatcgg ctgtaattgc aaatagttcg
     2641 gaagaaatgc gcgattccga attcccgaat tttactcttt tcaatattgc cgcaatattt
     2701 gtcctgagcg cccctgaata cacaagttcc ccttttttca aacgtataaa atctgtctta
     2761 ctggcaagag gtttgccatt cttgatggga attatatccg tagttgttga accgatgtca
     2821 acaaatatac aatccccgta ttcttttcct gtaaggagcg aagaggccat ccaattggct
     2881 gctgcaatac tgcgtttttt ctctgttgtg aggtcgccgt tattatcaag gaaaaaagca
     2941 ttttcaaaga cattattaac cgcatcaatg atatacgaaa ggcctgcatc tttatcagga
     3001 aaacaatcag cgagttctcc tgttatgacc actcctactt tttcaggttt caggcgctgt
     3061 gcaatttcaa gcagcatccc aggcagcatt gtgttcttcc agagagggat ataatgaagt
     3121 tcaactattt taccatcatc agaagcaatt tttgtgttcg ctccaccaat atcgatgcca
     3181 aggatcattg actattgagt aaacgtgatt gttaaaaagg tttatggaaa ttatttcagg
     3241 aaaagataga aattatttca atattcaaaa aaatgatttc accgtattga atccggtttc
     3301 aagcgccatt ttgagtatat caagcggatt tgttattcca agataatgga ccataatagg
     3361 tatcgccaca aaagtcccgg ccatactgcc aagatttgcc atcaaggcca caagaagaat
     3421 cctggataac ttattctgca ttaattcagt catggtttca gatttcatta tcgtcctgaa
     3481 gtcctcggtg gtgggaggac gaaaatgtgc ttccacaaac ccggctaacc atcctatggc
     3541 aaggaagggg tgcaagaacc cgaaccatgc aagaccaaaa gcggtcaatg cagagagtgg
     3601 gtgtcccctg acaacaagaa cacccaaagc tgaaagtatt ccctgggcca aaaaaaggta
     3661 tagcattgcc gtaagaaact tttcaagggg atatcccccg aaaaccagaa gaacaagcat
     3721 tgcaaggatc atcaatataa cagcaataaa tattgctttt agcca
//
LOCUS       DATGBN010000142         1790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_243795, whole genome shotgun sequence.
ACCESSION   DATGBN010000142 DATGBN010000000
VERSION     DATGBN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243795"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VKL21_03195"
     CDS             <1..894
                     /locus_tag="VKL21_03195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HMB44808.1"
                     /translation="HSHPRWIVEKWIKELGSVKEVEVLCRANNMVPPLTIRVNEFKSE
                     IASLQKKLDEEGYSSSRTGLSPFGLIIDRKEDIFKTRAFKEGFLEVQDEGSQLVTLLT
                     GAKPGELVIDACAGNGGKSLFLSSLMKNRGTIIASDLSPVKLANLRKRATKAGAFNIK
                     TANKESLNKYDKKGDCVFIDAPCSGMGVFRRNPDSKWRLTPDDVKELAAKQKEILSEF
                     SRLVKPTGRLVYATCTISKEENEDNVHGFLNENKDYYLVPVSEMPGFGFGKLISEDGF
                     FRSMPHIHNTDGFFGAVMIRR"
     gene            complement(902..1621)
                     /locus_tag="VKL21_03200"
     CDS             complement(902..1621)
                     /locus_tag="VKL21_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HMB44809.1"
                     /translation="MEELILIENLQKVFGEKEVLRNIDLKILQGEIFAFIGPSGSGKT
                     TLLRILNFFEIPEKGNIKFNGMNPEIINIRRRMSLLFQTPAVFNNSVFENVAYGLKVR
                     GIDKKIIEKKVGNALNIVGLPGMEKQNARTLSGGEAQRMAFARAIVYDPQVLLLDEPT
                     ANLDPANVIKIEDIIKRIRNERGTTIVLATHNIPQVRRIADRVGILLNGELIEVNSKE
                     GIFSKPKDTRSEAFIKGEMIY"
BASE COUNT          498 a          382 c          412 g          498 t
ORIGIN      
        1 cattcacacc cgcgctggat cgtggaaaaa tggatcaagg agctgggatc ggtcaaagaa
       61 gtagaggttt tgtgccgtgc caataacatg gtaccgcctt taacgatcag ggtgaatgaa
      121 tttaagtccg aaatagcatc acttcaaaaa aagcttgatg aagagggtta ttcttcttca
      181 agaacgggat tatcaccttt tggccttatc attgatagga aagaagatat ttttaaaacc
      241 cgggctttta aggaaggatt tctcgaggta caggatgaag gaagccagct tgttaccctg
      301 cttaccgggg caaaacctgg agaacttgtt atcgatgcat gtgcaggtaa tgggggaaaa
      361 tcccttttcc tttccagcct gatgaagaac cggggaacca taatcgcttc agacctgtcg
      421 ccggtgaaac ttgcgaacct gcgaaaaagg gcaacaaagg ctggcgcgtt taatatcaag
      481 acggcgaaca aagaatctct aaataaatat gataaaaagg gggattgcgt gttcatagac
      541 gcgccgtgtt caggcatggg tgttttcagg aggaacccgg attcaaaatg gcgattgacc
      601 ccggatgatg tcaaagaact tgcagcgaaa cagaaagaga tactgagtga attcagcagg
      661 cttgttaaac ccacagggag attggtctat gccacatgta cgataagcaa agaggaaaat
      721 gaggacaatg tgcatggttt tctcaatgag aataaggatt attacctggt tcccgtatcg
      781 gaaatgcctg gatttggatt tggcaaatta atctctgaag atgggttttt caggtcaatg
      841 ccacatatac ataatacaga tgggtttttc ggggctgtta tgataagaag gtaaaagaaa
      901 ttcaatatat catctctcct ttgatgaacg cctcagacct tgtatctttc ggttttgaaa
      961 aaataccttc tttgctattg acttcgatca attctccgtt caataatatc cccaccctgt
     1021 cagcaatgcg cctgacctgc gggatattat gcgttgcaag tacaatagta gtcccgcgct
     1081 cgtttcgtat tcgttttatg atatcctcta tctttataac attggccggg tcaaggtttg
     1141 cggtgggctc atcaagaaga agcacctgcg ggtcgtagac gatggccctg gcaaaagcca
     1201 tacgctgcgc ctctcctccc gaaagcgttc gagcattctg tttttccata ccgggaagtc
     1261 ccacaatatt taaagcattg cccacctttt tttcaatgat ctttttatca ataccgcgca
     1321 cttttaagcc atacgccaca ttttcaaaaa cggaattatt aaatactgca ggggtctgga
     1381 aaagaaggga catcctgcgt ctgatattga ttatttccgg gttcatccca ttgaatttta
     1441 tgttcccttt ttccggtatt tcaaaaaaat tcaagatacg aagaagcgtg gtcttgccgg
     1501 agccgcttgg tcctataaaa gcgaagatct ccccctgcag gatttttaga tcaatatttc
     1561 ttaagacctc tttttcaccg aataccttct ggaggttctc tatcagtatc aattcttcca
     1621 ctttagtccc tcctctggaa gaagttcgcc aggatattta cgccaagaac aagacagatg
     1681 aggatgatcc ccagggcgat ggatgttgag agatcaccct tgctggtttc cgatgtgatc
     1741 gcggtcgtga gagtgcgggt ttcggaaagt ccttcaccta ttcctcccac
//
LOCUS       DATGBN010000143         5364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_127650, whole genome shotgun sequence.
ACCESSION   DATGBN010000143 DATGBN010000000
VERSION     DATGBN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127650"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VKL21_03205"
     CDS             complement(<1..317)
                     /locus_tag="VKL21_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44810.1"
                     /translation="MDVIFFKDKKYSLKTLGLLTGLMDQDIENIHNNILIIAEVVDEP
                     DKLPYFLEEIKNLEIEDPEKFRFVMLRVQIDSQLHLNEDIEKYHKRLFVSQVIEKLIY
                     GELL"
     gene            371..1114
                     /locus_tag="VKL21_03210"
     CDS             371..1114
                     /locus_tag="VKL21_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HMB44811.1"
                     /translation="MDTPVILKIFHKGKMKEYFAKPEGEMHTDLGIIRLDELRSKNFG
                     DKISSHLGAEFVIRKPRAPDFFIHAKRSGAPMMPKDVGVIISNTGLCSSDHVLDAGTG
                     SGILAIYLGSIAKKVVTYEVREEFMEIARNNIALTGISNIECRHGDIVEEIGKLDEKF
                     DVITLDTIAAARVIPFVPDVIFPGGFLATYSPFLEQAKEIREAIGKTNFIEVKTLETM
                     EREISFTDRGTRPATARVGHTGYITIARY"
     gene            1189..2325
                     /locus_tag="VKL21_03215"
     CDS             1189..2325
                     /locus_tag="VKL21_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44812.1"
                     /translation="MMRFAFFCLLLLFGTNIATAESIITVNVNESTDALWTMEKYIHF
                     TKESDFSEWTKMDQNLSRYRNIPEYEEIIKSFQNSSVNFPNRSMEVENFTISYDKNIT
                     ISERLGIIRYSFVWKNFSYNDSGKIYIGDAIPGGVLFLSSGDQLIIRIPEGYNVTNVT
                     PVFDKKNGNRLIWFGTLYGNFSSGEPFVELSPWNTTNVSSAENSYVSRLAKWPILEIV
                     LIFISIMAVLAFVRLWKKKRSGDVSTADMVTQVPREMLHEISEIPGAWTKIVQSVQKE
                     FGEESIEELEKAAREQRLKNIGLNDTLLSEFSNEDLEDEEMIKQFILRSGGQAYQTDI
                     VEHSGLSKSKISMVLSKMKDDGMIIKIRKGKENLIRLVKEINEP"
     gene            complement(2363..3829)
                     /locus_tag="VKL21_03220"
     CDS             complement(2363..3829)
                     /locus_tag="VKL21_03220"
                     /inference="COORDINATES: protein motif:HMM:NF003231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HMB44813.1"
                     /translation="MNYQHLEPSAAGSRINQSELQWTEKYRPKTLSAVIGHNKALEEL
                     KIWAKSWVHGIPQNRAVVLYGRAGTGKTTTAHALARDMGWEVIELNASDQRTADIIDK
                     VAGSASRLGTLEGSGIKRLIIMDEADNIHGTADRGGERAIAEIIKNTGQPIILIANEL
                     YDMSTGLRTACKPIQFNSVMSRSMIPALKKIVETEGIKCDLGAIEKIAGNANGDLRSA
                     INDLQAIAQGKTSIQIGDIITGERDSKENIFKFLTKIFRSTNIREVHDASLNLDENPD
                     DLIQWMDENLSLEYTTPNDLAEGYHYLGRASVFLGRVKRRQNYNMWRYAGVLMTAGII
                     VARSHRNSGFVKYQPPSLWRKLGQTKGMRLVRDSTAKKIGTHCHVSMSYTRSQLFAFF
                     RNLVKDDDIAPKVVALLGLQPEEIAFLMESKSVTKKVQKIYETARSLIENETEHEVEL
                     FGGFGINIQKIQIPEPEKVEPGISIKKDSKAQSSLFDF"
     gene            3880..4965
                     /locus_tag="VKL21_03225"
     CDS             3880..4965
                     /locus_tag="VKL21_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMB44814.1"
                     /translation="MRLNVIQLLINSSIMMSNLFIPIFASELHATGTQIGIIGACYGG
                     ALFVSTYIFSRAADSYPPKTLLLAGFLSSSITFFIQIFARDPISLAIIRALAGFSVGI
                     YPAVLILYVYNLKQSIGKFSSFMPLGWAIGNLMAGIIAVYYQIFAVASFLFAVSFLLT
                     LKLPYTEAGTKKKTDFFSFELLKKNINIYFPFFLRQIGANGVWLIFPLYLQSLHANKF
                     WIGIIYMLNPALQFIIMRRLDKYENRFLIHTGDLLSAVAFIALIPLTIFYQAIPGLAL
                     IAFSYSFLYVGSINELIKNNEEKGAAAGLLNSSIALASILGSFTGGIILQYYGFRAVM
                     ATGAFFALLGYAVMLLADRSGIPQKSS"
     gene            complement(4919..>5364)
                     /locus_tag="VKL21_03230"
     CDS             complement(4919..>5364)
                     /locus_tag="VKL21_03230"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HMB44815.1"
                     /translation="PDGMILSGGPTLARAGNCSVYVKELDLPILGICLGHQVMAQTYG
                     GTVKTGAAGGYASIEIEILEENDILKGLGKKTNVWASHADEVSILPPDFIRLARSPLC
                     EVEAMKHRTKPLYGVQWHPEVSHTEKGNDILRNFFGVCQTYRPIA"
BASE COUNT         1515 a         1103 c         1078 g         1668 t
ORIGIN      
        1 agcagttcac catatattaa tttttcaatt acctgggaaa caaaaaggcg tttatggtat
       61 ttctcaatat cttcattgag gtgcaactgg gaatcgatct ggactcttag cattacgaac
      121 ctgaattttt ccggatcctc aatttcaagg ttttttatct cttccagaaa atatggtaat
      181 ttatcgggct catccacgac ttctgcgatg attagaatat tattatggat attttcgata
      241 tcctgatcca ttagacctgt aagaagtcct aatgttttca atgaatattt cttatcctta
      301 aaaaagatta catccataat aaccatgtat tctatatcta ttaagtccta tattacccca
      361 gcggagaaaa atggacacac cggtaatctt aaaaatattc cataaaggaa aaatgaaaga
      421 gtattttgct aaacctgagg gtgaaatgca cacagacctg ggcataataa gactggatga
      481 acttagatcg aaaaactttg gagataaaat aagttcccac ctgggagctg aatttgttat
      541 ccggaaaccc agggctcctg atttttttat tcacgcaaaa cgcagcggag ctcccatgat
      601 gcccaaggat gttggggtaa ttatttcaaa tacaggtttg tgctcttcag atcatgtact
      661 ggatgcagga acaggttcgg gaatacttgc gatctacctc ggttcaatcg caaaaaaggt
      721 tgtaacttat gaggtcaggg aggaattcat ggaaatagcc cgaaataata tagctctcac
      781 aggtatttcg aatattgaat gccggcatgg agatatagtc gaagagatag gtaaattgga
      841 tgagaaattt gatgttatta cccttgatac tatcgcagcg gcccgggtca ttccatttgt
      901 tcctgatgtt atctttcctg gcggttttct tgccacatat tcgccttttc ttgaacaggc
      961 aaaggagatc agggaggcca ttggaaaaac gaattttatc gaggtcaaga cccttgaaac
     1021 catggaacga gagatttcat tcacggaccg gggtacacgc ccagcaaccg ccagggttgg
     1081 ccacacaggt tatattacca tagctagata ttgattcatg ttaaaataaa gtatttacag
     1141 gaataactaa acagtaaatc ttaataccta tgtaattgtt tgatgtagat gatgagattt
     1201 gcctttttct gtttattatt actttttgga acaaatatag caacggccga atcgataata
     1261 acggttaatg ttaatgaaag cacagatgct ctctggacga tggaaaaata tatccatttt
     1321 acaaaggaat ccgattttag cgaatggaca aagatggatc agaacttgtc cagatatcga
     1381 aatatccctg aatatgagga aataatcaaa tcatttcaaa attccagcgt aaatttcccc
     1441 aaccgctcca tggaagttga aaactttacc atttcatatg ataagaacat aacaatttca
     1501 gaaagacttg gaattatcag atattctttc gtatggaaga atttttctta taatgattca
     1561 ggaaagatat atataggcga tgccatcccc ggtggtgtgc tatttctttc gtctggcgat
     1621 cagctaataa tcagaatacc tgaaggatat aatgtcacaa atgtgacacc tgtttttgat
     1681 aaaaagaatg gtaaccgttt gatatggttt gggacgcttt atggcaattt cagcagtgga
     1741 gaaccatttg ttgaactctc tccctggaat acaacaaatg tttcaagtgc agagaattca
     1801 tatgtatccc ggctggcaaa atggcctatc ctggaaattg tccttatttt catttccatc
     1861 atggctgtcc tggcttttgt tagattatgg aagaaaaagc gttcaggaga cgtatctacc
     1921 gcagatatgg taacgcaggt gccaagagag atgcttcatg aaatttctga aatccccggg
     1981 gcatggacaa aaatcgtgca atcagttcaa aaagaattcg gagaggaatc tatagaagaa
     2041 ttagagaagg ctgctagaga acaaagatta aaaaatattg gacttaatga cacactgctt
     2101 tctgaattct caaatgaaga tttggaggat gaagagatga taaagcaatt cattctaagg
     2161 tcggggggcc aggcgtatca aacagatatt gttgaacata gcggactttc aaaatccaag
     2221 atcagcatgg ttctctcaaa aatgaaggat gatggaatga tcataaagat aagaaaaggc
     2281 aaagaaaatc tcataagact tgtcaaagaa ataaatgagc catagaagct aatgaaaatt
     2341 ttctttgcgg gttcatttgc gatcaaaaat caaacaatga gctctgtgct ttgctatctt
     2401 tcttgatgga aatccctggt tctacctttt ccggttcagg aatttggatt ttctgaatat
     2461 tgatcccaaa tcccccgaat agctcaactt cgtgctccgt ttcattttct atcaaggacc
     2521 tggcggtttc atatatcttt tgtacctttt ttgtaacaga ttttgattcc ataaggaagg
     2581 caatttcttc gggttgaagt cccaataaag caacgacttt cggagcaata tcatcatctt
     2641 tcacaagatt cctgaaaaac gcaaaaagct gggaacgggt atagctcatc gaaacatggc
     2701 agtgcgttcc tatctttttt gcagtggaat ctcttaccag tctcatccct tttgtctgtc
     2761 cgagttttct ccataacgat ggcggctgat attttacaaa accgctgttg cgatgggatc
     2821 ttgccactat gataccggct gtcatcagta ctccggcata tctccacata ttgtaattct
     2881 gccttctttt cacccttcca agaaaaaccg acgctcttcc aagataatga tatccttctg
     2941 caagatcatt tggggttgta tattccaggg agagattctc atccatccac tgtatcaggt
     3001 catccggatt ctcatcaaga tttaaagatg catcatgtac ctcacgaata tttgtgcttc
     3061 tgaagatctt tgtgaggaac ttgaagatat tttccttgct gtccctttca ccggtaatta
     3121 tatccccaat ttggattgag gtcttaccct gtgctatagc ctgaaggtca tttatcgcac
     3181 ttcgaagatc cccgttagca ttcccggcaa ttttttcaat agcgccaagg tcacatttta
     3241 ttccttcagt ttcaacaatt ttcttcagcg ccgggatcat tgaacgtgac atcacggaat
     3301 taaattggat tggtttacag gctgttctaa gtccggttga catatcatag agttcatttg
     3361 ctattagaat gatgggttgg cctgtatttt ttatgatctc tgcaattgcc cgttcacctc
     3421 cacggtcggc tgtgccgtgg atattatcag cctcgtccat tatgataagc cgttttatcc
     3481 cgcttccttc aagcgtgccc aatcgggaag cagagcctgc aaccttatct atgatgtccg
     3541 cagtgcgctg gtcgcttgca ttaagctcga tgacctccca tcccatatcc cttgcaagag
     3601 catgagctgt tgtggtctta cctgttcctg cccttccata taacaccact gccctgttct
     3661 ggggtattcc atgcacccag gacttcgccc aaatcttaag ctcctcaagt gccttattat
     3721 ggcctattac ggcggataat gtctttgggc ggtatttttc ggtccattga agttcagatt
     3781 gattgatcct tgaacctgcc gcgctgggtt ccagatgttg ataattcatt aataatacta
     3841 tatcattata ttgcttaaat acataatcac ttgattagaa tgagacttaa tgtcatccag
     3901 cttcttatta attcctccat aatgatgtcc aatctattta tccctatttt tgcaagtgaa
     3961 ttacatgcca cagggacaca aataggcata ataggggcct gttatggagg agcgcttttt
     4021 gtttcaacat atatattcag cagggcggct gatagttatc ccccaaagac acttttatta
     4081 gctggtttct taagttcatc aataacattc tttatccaga ttttcgcgcg cgacccgatc
     4141 agtcttgcga taatccgtgc tcttgcaggc tttagcgtgg gtatttatcc tgctgtgctg
     4201 atcctgtacg tttataattt aaagcagagc atagggaaat tcagttcctt catgccgctt
     4261 ggctgggcaa taggaaacct gatggcaggt attatagccg tatactatca gatctttgcg
     4321 gttgcttctt ttttatttgc agtgtctttc cttctgacat taaaactacc ttataccgaa
     4381 gctggcacaa aaaagaaaac ggactttttc tcatttgaac tcctgaaaaa aaatattaat
     4441 atttattttc catttttctt gcgccagata ggggcaaatg gtgtctggtt gatattccct
     4501 ttatatcttc aaagtcttca tgccaacaaa ttctggatag ggataatata tatgttaaat
     4561 cctgcccttc aatttattat aatgaggcgg cttgacaaat atgaaaacag gtttctgatc
     4621 cataccgggg atttgctttc ggctgttgct ttcatagcat tgataccatt aacgatcttc
     4681 tatcaggcga ttccgggtct cgccctgatc gctttttcat attcgttcct gtatgtgggc
     4741 tcaattaacg aactcataaa gaacaacgaa gaaaaaggag ctgctgcagg attgcttaat
     4801 tcttcaatcg cactggcttc aatcttaggt tcattcacag ggggtatcat ccttcaatat
     4861 tatggtttca gggctgttat ggcaacgggt gcattttttg cattacttgg ttatgcagtc
     4921 atgctattgg ccgataggtc tggcataccc caaaaaagtt cctgagaatg tcatttcctt
     4981 tttcagtatg agagacttcc ggatgccact gcaccccata caggggcttt gtcctgtgtt
     5041 tcatggcctc cacttcacat agaggcgaac gggcaagcct tatgaaatca ggtggaagaa
     5101 tagacacttc atccgcatgg gatgcccata cgttggtctt tttcccaagt ccttttagaa
     5161 tgtcgttttc ctcaaggatc tctatttcaa ttgaggcata gccccccgca gcgcctgttt
     5221 ttacagtacc gccgtaagtt tgcgccataa cctgatgtcc caggcaaata ccaagtatgg
     5281 gaaggtcaag ctcttttaca tacacgctgc aatttcccgc gcgcgcaagt gtgggaccgc
     5341 cgctgagtat cattccatca ggat
//
LOCUS       DATGBN010000144         8029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_243862, whole genome shotgun sequence.
ACCESSION   DATGBN010000144 DATGBN010000000
VERSION     DATGBN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243862"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1006)
                     /locus_tag="VKL21_03235"
     CDS             complement(89..1006)
                     /locus_tag="VKL21_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB44816.1"
                     /translation="MKIDVGDRVLLTYLDREPVEFRVKGIISTGTFELDRYAIIATYD
                     TVKELIEKKEATNIVIRLEKPEESAKYKLLLQKETTLPNVKTWQELSAGMAGMIETFG
                     VISVMTSGVSVFVAAIAISLIIYTTVRNKIRQIGVLNMIGLLDTPTSGGMLVDGIEVS
                     SLDENSRADFRLKKMGFVFQFFSLLMELNSLENVALPMIMDKRKYDRANSLLELVGIG
                     DRAAHYPSELSGGQQQRVAIARALANEPVILLADEPTANLDTESSNQIVGLFRELNEK
                     GQTIVMVTHELELGEKADRIIRIKDGKVV"
     gene            complement(1006..1638)
                     /locus_tag="VKL21_03240"
     CDS             complement(1006..1638)
                     /locus_tag="VKL21_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44817.1"
                     /translation="MTAYLESNLSGDNIAYLGEIKKDDYSNAIFTLDSDGSGKKTGFL
                     RINYEDDFGKHEIQKDVIMVINPTESQNLLPLLLGIIAIAVIIFFIKRRRETLMVILA
                     ITIGVVGPLFTTALNNGMQDAFVGNTVNVYTGHLQIQPNTGDSVISKSESVLSKVTGI
                     KGIVGAAPRMTGGVDIESRTEKMGQKRWKEENSLMTRINMNCWLALLLQK"
     gene            complement(2198..2854)
                     /locus_tag="VKL21_03245"
     CDS             complement(2198..2854)
                     /locus_tag="VKL21_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HMB44818.1"
                     /translation="MLKAVIFDMDGVLVDSMPYHADAWITVFAEVGIKIKREDIYEIE
                     GSNHTGIINLIFKKAGRIPAPSDFNKLAEEKKKIFSKINKARVFNGIYECIDLLKNIC
                     LLGVVSGSDKAIVKELIERFFPDTFSVLVTGNDVSEGKPSPEPYLKAVEMLKVGKHEC
                     IVIENAPMGVESAKRAGLYCIAIPTYVDPGILKNADMIISNQKMLKEFLKNSIRNKDC
                     "
     gene            2911..3333
                     /locus_tag="VKL21_03250"
     CDS             2911..3333
                     /locus_tag="VKL21_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HMB44819.1"
                     /translation="MKAYDFDFPEDRYYMKNHVWLKPLNGEILLGITELGQSLSKGIV
                     HIDLPETGESIKKGDMLVAYETIKAVSQISVPFDAKVTSVNQKLWDDPNIINSDPYNE
                     WIVKVKGEFDDSHVMKVEQAIKYFTELITKERERYADN"
     gene            3379..3807
                     /locus_tag="VKL21_03255"
     CDS             3379..3807
                     /locus_tag="VKL21_03255"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HMB44820.1"
                     /translation="MVEIEGHKLPEELYYTKDHTWARIEDNGTVTVGLDAYGAKAAGN
                     IEFIDLPLEDDEFEAGEAFGSLESAKWVGSLLMPVAGTILEVNENIEDDLELLKEDPY
                     GEGWLIKLKPGNLKDDLKGLIHGLDVGPWLKKEIESRKKK"
     gene            complement(3830..4084)
                     /locus_tag="VKL21_03260"
     CDS             complement(3830..4084)
                     /locus_tag="VKL21_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44821.1"
                     /translation="MPVEISQTQLPSLLLLTALLVAVVVAVYLSLKSGDKYSVEDAQR
                     DAISFADVVREADGPMTSFLIAAFIIVIIWAIAYLILYLK"
     gene            complement(4074..4271)
                     /gene="ccoS"
                     /locus_tag="VKL21_03265"
     CDS             complement(4074..4271)
                     /gene="ccoS"
                     /locus_tag="VKL21_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HMB44822.1"
                     /translation="MLADHIMTGGSMPIDTADYSIILISSVIILLIFPAILYWGWRKG
                     QFRDIEEAKYRMIEMEEENAR"
     gene            complement(4273..6921)
                     /locus_tag="VKL21_03270"
     CDS             complement(4273..6921)
                     /locus_tag="VKL21_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMB44823.1"
                     /translation="MNAMEFKEHSSAKNFLLSSIFWLILFTSFGFIIAIKFFAPEFLG
                     QLSFLTFGRMRPMHVNGVTLGFLSTGLIGIAYYIIPRLSGTKLYSEKLGNLTVILWNL
                     AIASGTVLLALGYTQAREYAEYIWIIDVAIMITLLLIGYNIFRTIQKRTERKLYASTW
                     YVMGTFLSFPIVYFIGNVMWHPDTGSLQGAVDGVFNWFYGHNVLGLWFTTLGIAGWYY
                     FIPIMTRRPLYSHLLSMIGFFSIVFVYTGVGGHHLLQAPIPEWLKTIAIVNSGLMMVP
                     VLAFITNILLTMRGKWDYFIESIPLRFILIGAFLYFLASAQGTFQAFRETNMYLHFGQ
                     WAVGHAHIALLGGFGFLVMGAIYFLIPRITGKEIYSKRLMSYHFWFTMLGFILFFSIM
                     TIMGLIQSSGWVQNITVATVLLQLKPYFIIRALAGGTIIAGQYIFFYNILMTFRSSAN
                     TASEPSLAPQKVQRKSRKVQKYRESAPLFAIGALGLFLTMFFITVILPYLLMDYPPSD
                     ISHPYSDEQGRGREIYKSNGCLYCHSQFVRPQDWGLGRTSLPGDYFYDRPHLFGTERT
                     GPDLAQIGGQRPPLWEIMHHRNPRSVSPGSIMPNFSYLSDQDLQDLKAYIEGLGNRNL
                     ETQDFQPLVPELYSGRDNIYNNTIANANSSNESITEYADLILAGKILFSQRCLPCHGA
                     SGIGQGPYARHVNQHPANLNDRISNFPGVDYNFWRVMEGVPGTAMPPWKLSLTEDAVW
                     KIISYEKTFVDGVVRVVPGDFSDGEAEDFGNRGIKSPVVKDDINFSEGMKLFNLYCAQ
                     CHGIDGQGDGPASVYINPEPANLTETNNDFKTQGQWFWKVSEGVETTNMPPWKYVLTD
                     DDRWKAIYYVQKNFSEPGIFDEKWRS"
     gene            6960..7115
                     /locus_tag="VKL21_03275"
     CDS             6960..7115
                     /locus_tag="VKL21_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44824.1"
                     /translation="MVAKGDTISASTKVTAIDSEVVSPVPELSSLVFISIGLLGLFGL
                     IRIKKKS"
     gene            7158..7740
                     /locus_tag="VKL21_03280"
                     /pseudo
     CDS             7158..7740
                     /locus_tag="VKL21_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
BASE COUNT         2323 a         1756 c         1629 g         2321 t
ORIGIN      
        1 agcgtcatat ggtacaaacc ccttaacgaa aactggaatg acaagcctca cagctttgag
       61 cgtgaggtcg ctgacgattg ccataggatt aaaccacttt cccatccttt atcctgatta
      121 tcctgtccgc tttctcgcca agttcgagtt catgcgtcac catgacgata gtttgccctt
      181 tttcgttcaa ttcacgaaat agtccaacta tctggttcga ggattcagta tcaagattcg
      241 ctgtaggctc atcggcaagc aaaatgacag gctcattcgc gagtgcccgc gcgatcgcca
      301 cacgctgctg ctgcccccct gaaagctcgc ttggataatg ggccgccctg tcgcctattc
      361 caacaagttc cagaagtgaa tttgcgcggt catattttcg cttatccatt atcatcggga
      421 gcgcaacatt ttcaagggaa ttcaattcca tgagcagact gaagaactgg aaaacaaacc
      481 ccattttctt aagccggaaa tcagccctgg agttttcatc cagtgaggac acttctattc
      541 catcaaccaa cattcccccc gatgttggcg tgtccagaag tcctatcata ttcaggactc
      601 caatctggcg gatcttattt cggaccgtag tgtatattat aaggctaatt gcgatcgcgg
      661 cgacaaatac cgagactccc gatgtcatca cggatatcac cccaaaggtc tcgatcattc
      721 ctgccatacc ggccgaaagt tcctgccatg ttttgacatt aggcaaagtg gtctctttct
      781 ggaggagcaa tttatatttc gcggattcct cgggtttctc aagccttata acgatattgg
      841 tggcttcttt tttttcgatg agttccttaa cagtatcata tgtcgctata atggcatatc
      901 tgtcaagttc aaatgtacct gtgctgatga tacctttcac cctgaactcc acaggttccc
      961 tatccagata tgtgagaaga accctgtccc caacatctat tttcattact tctgcaaaag
     1021 aagtgccaac cagcaattca tatttatcct tgtcatcaag gaattctcct ctttccagcg
     1081 tttttgaccc attttttcgg ttcttgattc gatatccact ccgcccgtca ttctgggcgc
     1141 agcccctact atacctttta tccctgtgac ttttgacagc acgctttctg atttcgaaat
     1201 aacgctatcg ccggtatttg gctggatctg caaatgccct gtataaacat taaccgtatt
     1261 tcccacaaac gcatcctgca tcccgttatt cagggctgtt gtaaatagag gcccaaccac
     1321 tcctatcgtt atggcaagta ttaccatcag ggtttcacgt cttcttttaa tgaaaaatat
     1381 tattactgct attgctatta ttcccaataa taacggcagg agattttggc tttctgtggg
     1441 attgataacc ataataacat ctttttgtat ttcgtgtttt ccaaaatcat cttcataatt
     1501 tatccgcaaa aaacctgttt ttttcccgct gccatctgaa tcaagtgtaa atattgcatt
     1561 tgaataatca tcttttttta tctcgccaag ataggcaata ttatcccccg aaagatttga
     1621 ttcaagataa gctgtcactc cttttgcatc ccctgtaccg gtattttcgt ttatttaaag
     1681 attacggatt cgcttttttt ataaccaatt ctataatcct tgaggatttc tgccgaaaag
     1741 gtttggtcat aataataata acgattataa ttatataatc atttagatat atttaaatag
     1801 ataaaggtca tatttagagt aggtgtgcat gagatgacca gtttaaaaga ttgcttgaaa
     1861 acccgggaaa accaggatga aatagataag aagtatattt ttctatataa attaaaatct
     1921 ttgataggtg gaaaatccgg atacatacgg tttattatat tgtttggaat attattcaca
     1981 atcctgcaaa taactgacct tgttatcaca tatttcgcac ttcaaaatcc actaaataaa
     2041 gaactcaatc ccttgtataa tcaggaatgg ttcatcccct tcaaattaac aatggtcctt
     2101 ctcattatgt cggttatgca acgaattccc gccccgaaca gaagattggc taaaggagca
     2161 atgataggaa tgatatatat gtatttgttc ataaatttca acaatcttta tttcttattg
     2221 agttcttaag aaattctttt aacatttttt ggtttgaaat aatcatatct gcattcttca
     2281 atatccccgg atcaacataa gttgggatcg caatgcagta taaacccgcc ctttttgccg
     2341 attccacacc cattggcgca ttttcaataa cgatacattc atgttttccg actttaagca
     2401 tttcaacagc cttcaaatat ggctcaggag atggttttcc ttctgaaaca tcatttccgg
     2461 ttaccagaac cgaaaatgta tcagggaaaa acctctcaat taactctttg acaattgctt
     2521 tatcagaacc ggatactacc ccgagaaggc agatgttttt taatagatcg atgcactcgt
     2581 atattccatt aaaaaccctc gccttgttta tttttgaaaa tatcttcttt ttttcctccg
     2641 ccagcttatt aaagtccgaa ggcgccggta ttctaccggc tttcttgaaa atcagattga
     2701 ttattcctgt atggtttgag ccttcaattt catagatatc ctctcttttt attttaatcc
     2761 caacttcggc aaaaacagtt atccatgcgt ctgcatgata tggcatcgaa tcaacaagca
     2821 cgccatccat atcaaaaatc actgctttta acatcgttac ctaacgaggc gtcaaaagta
     2881 ttttatacct ctcttcagat tgtagacaat atgaaggcat atgattttga tttcccggaa
     2941 gatcggtatt acatgaaaaa ccatgtgtgg ttgaaacctt taaatggaga aatcctctta
     3001 ggtataactg aacttggcca atcattatcc aaagggatag tacatatcga tctacctgaa
     3061 acgggagaat caattaaaaa aggggacatg cttgtggcgt atgaaacaat aaaggcagtg
     3121 tcccagatat cagttccatt tgatgcaaaa gtcacatcgg taaatcaaaa actatgggat
     3181 gaccccaata tcatcaatag tgacccttac aatgaatgga tcgtgaaagt aaaaggtgaa
     3241 tttgatgaca gccatgtaat gaaagtagaa caggcaataa agtattttac cgaattgatc
     3301 acaaaagaac gagaacgata tgcagataat taactttata tacgtcaatg taataaccta
     3361 acaaaaagag gaaaaacaat ggtcgaaata gagggacaca aacttccgga agaattgtat
     3421 tatacaaaag atcacacatg ggcaagaatt gaagacaatg gtactgttac ggtcggtctt
     3481 gatgcttacg gagcaaaagc ggcgggaaat attgaattca ttgacctgcc ccttgaagac
     3541 gatgagttcg aggcaggcga agcattcggg tcgcttgaat ccgcaaaatg ggtgggcagc
     3601 ctattgatgc ccgtcgcagg tactattctt gaagtcaatg aaaatatcga ggatgatctt
     3661 gagctcttaa aggaagatcc ctatggtgaa ggttggttga tcaaattaaa gccgggcaat
     3721 ctgaaggatg acctgaaggg tcttattcat ggactcgatg tgggtccatg gcttaagaaa
     3781 gagatcgaga gtcggaagaa gaaataatgt aaaatttttg atttattttt catttaagat
     3841 atagaataag ataagcaatt gcccatataa taacgatgat aaatgcagcg atcaggaatg
     3901 atgtcatcgg cccatcagct tccctgacaa catctgcaaa gcttatagca tcacgctggg
     3961 catcctcaac cgaatattta tcaccgcttt ttaagctcaa ataaaccgcg acaacaactg
     4021 ctacaaggag cgcggttaac aggagaagcg aaggcaactg ggtttgcgat atttcaacgg
     4081 gcattttctt cctccatttc gatcatcctg tattttgcct cttcgatatc cctgaactgc
     4141 ccctttctcc atccccagta aagaattgcc ggaaagataa gaagaattat cacgcttgag
     4201 ataagtatta ttgaatagtc ggcagtgtca atgggcatcg atcctccggt cataatatgg
     4261 tcggcgagca ttttaagacc tccatttttc atcaaaaata cctggttcgg aaaaattctt
     4321 ctggacataa tatatggctt tccatcggtc atcatccgtc aggacatatt tccacggcgg
     4381 catattggtt gtttccacgc cttcgctcac cttccagaac cactgcccct gtgtcttgaa
     4441 atcattattg gtctctgtca ggtttgcagg ctcgggatta atatatactg acgcggggcc
     4501 gtcaccctgc ccgtcaatac cgtggcactg tgcacaatag agattgaata atttcatccc
     4561 ttcggaaaaa ttaatatcat ccttaacaac tggtgatttg atacccctgt tgccaaaatc
     4621 ttctgcttca ccatcggaaa agtcaccggg aacgaccctt accaccccgt caacaaatgt
     4681 cttttcatat gatattatct tccatactgc atcttctgta agcgagagtt tccatggggg
     4741 cattgccgtt ccaggtactc cttccatgac acgccagaaa ttgtaatcaa cacccgggaa
     4801 atttgagatc ctgtcattca ggttggcagg atgctggtta acatgccgcg cataaggtcc
     4861 ctggccaatg ccggaggctc catggcaggg caggcagcgc tgggaaaaaa gaatttttcc
     4921 agctaatatc aggtcggcat attctgtaat agattcatta ctggaattag cgtttgcgat
     4981 ggtattatta tagatgttat cccgtccgga ataaagttcc ggaacaaggg gctggaaatc
     5041 ctgcgtttca agattcctgt tcccaagtcc ttcgatatat gctttgagat cctgcagatc
     5101 ctggtcggag aggtacgaaa aattaggcat aatggagccc gggcttaccg agcgtggatt
     5161 cctgtgatgc ataatttccc acaaaggagg tctttgtccg cctatctggg caagatcagg
     5221 tccggtcctt tctgttccga aaagatgcgg gcggtcatag aaataatctc ctggcaggga
     5281 tgttctccca agaccccaat cctggggcct tacgaactgg gaatgacaat acaggcagcc
     5341 attgcttttg taaatctccc gcccgcgtcc ctgttcgtct gaatagggat gggagatatc
     5401 cgaaggtgga taatccataa gaagatacgg gagtatgact gttatgaaaa acatggtcag
     5461 gaagagccca agcgccccga tggcaaataa tggcgcgctt tccctgtatt tttgaacctt
     5521 tcgggatttt ctttgaacct tttgtggtgc aagagaaggc tctgaagcgg tattagccga
     5581 tgaccgaaaa gtcatcagga tattataaaa aaatatgtac tggcctgcaa ttattgttcc
     5641 gccggcaagc gctcttatta taaaatacgg cttcaattgg agcaatacgg tggcgacggt
     5701 gatattttgc acccatcccg aactctggat gagacccata atggtcatga tcgagaagaa
     5761 caatatgaaa ccaagcatgg tgaaccagaa atggtagctc ataagccttt ttgaataaat
     5821 ctccttaccg gtgatcctgg ggataaggaa atagatggcg cccatcacaa ggaacccgaa
     5881 gcctcccaga agtgcaatat gagcgtgtcc cacagcccac tggccaaagt gcaggtacat
     5941 gttagtttcc cggaatgcct ggaaagtgcc ctgcgccgat gcaaggaaat ataaaaatgc
     6001 gcctatcagg ataaaacgaa gaggaatact ttctataaaa taatcccatt tcccgcgcat
     6061 ggtaagaagg atattggtga taaaagcaag aaccggaacc atcatcagtc cgctattcac
     6121 aatagctatg gtctttaacc attcaggaat cggcgcctgc aggagatggt gtcctcccac
     6181 gcctgtatag acaaatacga tagaaaaaaa tccgatcatt gaaaggagat ggctgtaaag
     6241 cggacgcctg gtcatgatgg gaataaaata ataccatccg gcgatcccca gcgttgtaaa
     6301 ccataatccc aggacattat gcccgtagaa ccagttgaaa acgccatcaa ccgccccctg
     6361 cagtgaacca gtgtcgggat gccacattac atttcctatg aaatatacta tgggaaagga
     6421 aaggaatgtg cccatgacgt accaggtgga ggcgtacagt tttctctcgg tccttttctg
     6481 tattgttcta aaaatattat atccgatcaa taagagggtg atcattatgg cgacatcgat
     6541 tatccagata tattccgcat attcccttgc ctgggtataa cccagagcaa ggaggacggt
     6601 ccctgaagca atggcaagat tccacaaaat gaccgtgaga ttgcccagct tttcactgta
     6661 cagcttcgtt cccgatagcc tggggattat ataatatgcg atgccgataa gcccggtgga
     6721 aagaaaaccc agggtcactc cgtttacatg catggggcgc atgcggccga aagtgagaaa
     6781 tgataactgt cccaggaact caggagcaaa aaattttatg gcgataataa aaccaaatga
     6841 tgtaaaaagt atcaaccaga aaatcgagga taaaaggaaa ttctttgctg aactgtgctc
     6901 tttgaattcc atggcattca taatagttta atttttcaga tggttattaa agttatctga
     6961 tggtagcaaa aggagataca atatcggctt caactaaagt tactgctatt gactctgaag
     7021 tcgtttcccc tgtaccggaa ctttcttcat tggtatttat ttcaataggt ctccttgggc
     7081 ttttcgggct tatcagaatc aagaagaaaa gctaagaaat ttaaggaatc aaatcaatcc
     7141 ccttgatcag gtcaaatatg tcacgtctag tccaccagag aattaccact tcctttattt
     7201 ttctgctatt gttgttactt ggagtggttt tttttctcat cagcatagac ctgatcggtc
     7261 ttgctttctt aaagcttggt tttcctccca agtattccgt ccttctctta tttttatcgc
     7321 taataggcag ctatattaat atccccataa aggagataat ttcaagggaa ccaggtatct
     7381 caggcaggat cataaatttc tattggttca ggtatattat cccgcctctt gaggtagaac
     7441 atcgcaccat aattgcggta aacctgggag gaggagtaat tccggtcggt gaaaggactc
     7501 gggatagctg ttcctgcttt acttccccct atcgcagctg ccggcctggc tttattgatc
     7561 tcccctcaaa atgcgcccgt gatagcgtat atctcaggca cgcttggcag ccttattggc
     7621 gctgatctgt tgaacctgaa aaagatccct ggtcttgggg cacaagtggc atcaatcggg
     7681 ggagcaggga catttgatgg gatattcctg acaggaataa tttctgtgtt gctggcttaa
     7741 ttgatccata tttttaattt cagacaggat ttacaggaga gacttacaaa aagacctgta
     7801 ggcaggaata cttaggatat taccgagggg ccttatagct tttttgtgaa taatttcacc
     7861 agtttctaaa gcttattcat ttcttcttta ataacctctt ctaaacccct atttccctca
     7921 acatattttc taatagcaga atacgctggt aaagtagtag aattggttaa tcctaacgga
     7981 acatcgcaga cctgtatatg tggtcaccac gtgccaaaag acctgtcag
//
LOCUS       DATGBN010000145         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_437958, whole genome shotgun sequence.
ACCESSION   DATGBN010000145 DATGBN010000000
VERSION     DATGBN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437958"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /gene="mgtA"
                     /locus_tag="VKL21_03285"
     CDS             <1..1856
                     /gene="mgtA"
                     /locus_tag="VKL21_03285"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HMB44825.1"
                     /translation="SERLKLQQPETEFERGVRRFGYFLMEITLLLVIAIFAINTYMSR
                     PILEALLFSLALAVGLTPQLLPAIISVNLAHGAKRMAEQKVIVKRLASIENFGSMNVL
                     CSDKTGTLTEGVVRVNSTLDVEGKESEKVLFYAYLNAFFETGFTNPIDSAIRNYRQFD
                     VSGCRKMDEVPYDFIRKRLSILVSKDDKHLIVTKGALQNVLAVCSSAEISDEKIVDIA
                     TVKEQIQQCYEKHSSKGFRILGVAYRDVGYDQVITKDHETEMTFSGFLVLFDPPKAGI
                     ADTIKKLNQLGISLKIITGDNKLVAANVSQQVGFSSPQLLTGSDLRSMSDEALGKRVN
                     DISIFAEVEPNQKERIILALKKSGNVVGYIGDGINDASALHAADVGISVESAVDVAKD
                     AADIVLLEKDLGVLVQGVREGRMTFANTLKYVFMATSANFGNMFSMAGVSLFLSFLPL
                     LPGQILLTNLMTDIPEMTIATDSVDEEMVDHPRRWDIGFIRNFMLVFGVLSSVFDYLT
                     FGALFFILHATPGQFRTGWFMESVISASMIVLVIRTKKPFFKSKPGKYLFWATLLIAA
                     IALLFPFMPIGKVFGFSQLPISFLLLMGLIVAFYIITAEMVKGIFYRNVKF"
     gene            2077..2346
                     /locus_tag="VKL21_03290"
     CDS             2077..2346
                     /locus_tag="VKL21_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44826.1"
                     /translation="MTNLLIDDPAIELIRNELIKENAFAMRIFIGGGGCCERFEVIPV
                     KKALAGDITFMKGGIRILVEKKIVENTSGIRIKYDEHKGLLIDLT"
     gene            2416..3621
                     /gene="ahbC"
                     /locus_tag="VKL21_03295"
     CDS             2416..3621
                     /gene="ahbC"
                     /locus_tag="VKL21_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HMB44827.1"
                     /translation="MIGISKLYCGTVEPSDALRYGRDSSKLPSHLLQFSKDKKPVVVW
                     NMGRRCNLKCVHCYAQSKDIEYKNELTTQQGKELIDDLAEFGAPVILFSGGEPTMRKD
                     LPELALYARSKGMRAVISTNGTLIDKKMARVLKDIGLSYVGVSLDGMRETNDKFRGMK
                     GAFDAALNGMRNCLAEGVKVGLRFTINKQNVKDIPAIFDLLEKEKIPRVCFYHLVYAG
                     RGSKMVEQELSHEESRKTLDIIMDRTKALHDKGFPIEVLTVDNHCDGPYVYFRLLKED
                     PKRAGEVYELLQMNQGNSTGIGIGCVSWDGSVHADQFWRHYSFGNVKERKFSEIWTDT
                     TNELMAGLKNRKPLIKANADRCAHCKWFDICNGNFRVRAEAIYGNVWADDPACYLTKE
                     EIGYDQTGY"
BASE COUNT         1075 a          802 c          933 g          985 t
ORIGIN      
        1 tttccgaaag gttgaaactg cagcagcctg agacggagtt tgagcggggt gtgcggcggt
       61 tcgggtattt tctcatggaa attacgctcc tgcttgtaat cgcaattttt gctatcaata
      121 cctatatgtc acgccctatc ctggaagctc ttctattttc tttggctttg gctgttgggt
      181 taactcctca actgctaccg gcgataatca gcgtgaacct tgcacacggc gcaaagcgca
      241 tggcagagca aaaagtgatc gttaaacgcc ttgcctccat tgagaatttt ggcagtatga
      301 atgtgctctg ttcagataag actggaacac taaccgaagg agtggtgaga gtgaattcca
      361 ctcttgatgt tgaagggaaa gaaagcgaaa aagttctttt ctatgcttat ctcaatgctt
      421 ttttcgaaac aggtttcaca aacccgatag acagcgcgat caggaactat cgccagttcg
      481 atgtgtccgg ttgccggaaa atggatgaag ttccatatga tttcattcgt aagcgattga
      541 gcatccttgt ctcaaaagat gataagcatc tgatagtgac caaaggggct ctgcagaatg
      601 tgctggctgt gtgttcttca gcagaaattt cagacgaaaa gatcgtggat atagctaccg
      661 tgaaggaaca gatacagcag tgctatgaga agcacagcag taagggcttt cgcatattgg
      721 gagttgccta ccgggatgtg ggatatgatc aggttataac caaagaccat gaaacagaga
      781 tgactttttc aggtttcctc gttctgtttg atccgccaaa agccggaatt gccgatacga
      841 tcaaaaaatt aaatcaactt ggcatctcat tgaagatcat tacaggagac aataaactgg
      901 tcgcggccaa tgtcagtcaa caggttggtt tttcgagtcc gcaactcctc acaggttcgg
      961 accttcgatc aatgagcgat gaggcgctgg gcaaacgagt gaacgatatc agtatcttcg
     1021 cagaagtcga gccaaaccag aaagaacgaa tcattctggc cctgaaaaag agtggaaatg
     1081 ttgtcggcta tattggagat ggaattaatg acgcttcagc cctgcatgct gccgatgtgg
     1141 gcatatctgt tgaaagcgcc gtggatgttg ctaaagatgc tgccgatatt gtgctgctgg
     1201 aaaaggattt aggtgttctt gtgcagggtg tgcgggaagg gagaatgaca tttgccaaca
     1261 ccctgaaata tgtgttcatg gccacgagcg ccaatttcgg gaacatgttc agcatggctg
     1321 gagtatcgct cttcttgtcc tttctccccc tgctgccagg acagatcctt ctgacaaatc
     1381 tcatgacgga tatcccggag atgacaatag ccactgatag cgtggacgag gaaatggtcg
     1441 atcatcccag gcgctgggac attgggttta tccgcaattt tatgctggtc tttggtgttc
     1501 tgagttccgt attcgactat cttacattcg gggctttatt tttcatcctc cacgcaacac
     1561 ccggccaatt caggactggc tggttcatgg aatccgtaat ttcggcttca atgatcgtac
     1621 ttgtgatccg caccaaaaaa ccattcttca agagcaaacc cggaaaatat ctgttctggg
     1681 ccacattgct gatcgctgca attgcccttc ttttcccttt catgccgatc gggaaggtat
     1741 ttggatttag tcagctaccg atttcgttcc ttcttcttat gggattgatc gtggcctttt
     1801 acatcatcac ggcagaaatg gtaaaaggga tattttatag gaacgtgaaa ttctgattga
     1861 tatctaataa ttggaacctc agattccgag tcgcgcctct caaagggcgc agacacgtat
     1921 gctccgcagg catacgtgga gtcccgctcc cgaagggtaa tcgggacgcc gatgaacgca
     1981 tattgaatga tttatctaat gataatagca aacagtttaa tatccggaaa gatgtattat
     2041 ttttatacat aaaatgatga atacggaaca ggataaatga caaacctttt aatagacgac
     2101 cctgccatag aacttataag gaacgagttg ataaaagaaa atgcatttgc gatgcgcatt
     2161 ttcataggag gcggcggatg ctgcgagcgt tttgaggtca tccctgtgaa aaaagctctt
     2221 gcgggagata taactttcat gaagggcggg atcaggatac tcgtggaaaa gaaaattgtg
     2281 gaaaatactt caggtatccg gataaaatat gatgaacaca aaggattatt aattgattta
     2341 acataataaa gtaatccgga taggtttata taaaataaat tcgtaattat acgaacataa
     2401 attggagaac aaaaaatgat aggtatttca aaactttatt gcgggactgt tgaaccttct
     2461 gacgctctgc ggtacggacg tgactcaagt aaactccctt cacatttact gcaattctca
     2521 aaagacaaaa aaccagtggt tgtctggaac atgggcaggc gctgcaacct gaaatgtgtg
     2581 cattgctatg cccagtcaaa ggacattgaa tataagaacg aactcacgac ccaacagggc
     2641 aaggagctca tagatgacct tgcagaattt ggcgcgcctg ttattctatt ctcaggcggc
     2701 gaacccacaa tgcgcaaaga ccttcctgag cttgctctct atgcaaggtc aaaagggatg
     2761 cgtgcggtca tttccacaaa cggtacgctt atagataaga aaatggccag ggtcctgaaa
     2821 gacatcggcc tgtcttatgt tggggtttca cttgatggca tgagagaaac gaacgataag
     2881 ttcagaggca tgaaaggcgc attcgatgcg gcgctaaatg gtatgcgaaa ttgtcttgcc
     2941 gaaggcgtca aggtgggatt gcgttttact atcaacaaac aaaatgtgaa ggatattcct
     3001 gctatttttg accttcttga aaaagagaaa attcccaggg tatgtttcta tcacctggta
     3061 tatgcaggcc gcggctcaaa aatggttgag caggaactct cgcatgagga aagcaggaag
     3121 actcttgata taataatgga taggacaaag gcactccatg acaaaggttt tccgatcgag
     3181 gtattgacgg ttgacaacca ttgcgacggc ccgtatgtct atttccgcct cctgaaagaa
     3241 gacccgaagc gcgccggaga ggtgtatgaa ctcctgcaga tgaaccaggg caactccaca
     3301 ggtattggca tcggctgcgt atcctgggat ggttcggtgc atgctgacca gttctggaga
     3361 cactattcct tcgggaacgt aaaggaacgc aaattcagcg agatctggac tgataccacc
     3421 aatgaattaa tggcaggact taaaaaccgc aagcccctta taaaggcaaa cgcggacagg
     3481 tgcgcccact gcaaatggtt cgatatatgc aatgggaact tccgcgtgag agccgaggca
     3541 atatacggca atgtgtgggc ggatgaccct gcatgctact taacaaagga agagatcgga
     3601 tacgatcaaa cgggatattg agacctctcc acgaatgaag tcgggagatt ccagagttca
     3661 tataacctct ctgtggctct catcttcatt gattcggatt acatcatcca cagcctcagt
     3721 tatagtcgct tctgatttaa cggacgatcc gctcagtctc cggcctactc cgaagctggc
     3781 aggaaggatt ttccc
//
LOCUS       DATGBN010000146         6895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_437984, whole genome shotgun sequence.
ACCESSION   DATGBN010000146 DATGBN010000000
VERSION     DATGBN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437984"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1235
                     /locus_tag="VKL21_03300"
     CDS             111..1235
                     /locus_tag="VKL21_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0097588 - archaeal or bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlaD/FlaE family flagellar protein"
                     /protein_id="HMB44828.1"
                     /translation="MFEDISEKLKRFKMPEESLIKKIERIKKTPDAGISSASVSNPSS
                     VSAGSPPNAPDISEQVKKAQEEMTHKIEEIVKSKTEQINQSVEAIKEFDVKINKLEGA
                     VSNTKSSVGEFKDRLDKIDESLLELLSLYEVVSSTVNPFVGEKGIIPNEKLDQMEKRI
                     DILSQKPSGIPANIKEEYDIKFKALEGSLEELTQLIDSAPPNQEDMIRNVSEQVMERI
                     KPLIEQMKPMIEQHLQQTASQTSSGTPGSADTTATRYPDENIKLQYLDNKAETSIILL
                     NWIEFLLEKVGRNNLSEVLEYYIDIGWISEDVCEKMLAYANGIDYYVERPTWKLLPDD
                     HTKSLMFIEQLKGRKLEKSMLSKLERDVEKVIRTNEIYVS"
     gene            1242..2720
                     /locus_tag="VKL21_03305"
     CDS             1242..2720
                     /locus_tag="VKL21_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HMB44829.1"
                     /translation="MRRGISSYIRAWTYQDIILAGLIFTYIILMSRLVFDHYDALYVY
                     SLDFGVFDQALWSTSQGDFMWQSQVGLGSNWGIHFDPLLLLTVPIYYIFPDGRFLLVI
                     QTIFLALGAIPLYLIAKIKFESRWIPLAFPISYFIYPYLHNVNLFDFHSVMFSPLLIG
                     LTWYSIELKNIRLFTVFALLSLFIKENIFVIVILLCIYAFFKTEYKWEATILAAISII
                     YGIVVIKYVIPFFSGNTYMFIKNNTVSGIITNPPIDNFLAITQELFKPLLYLPLLNPL
                     PYLTGSILIMWHSARDISLFLTYHYPADVISLVMISVILGVANIPNYINFISKTYFKQ
                     EQKSIPKPKLIQLVIVSLMLISSIHSFYTTSQVMNMPALQYQQTEHDRIGIDIAQRIP
                     PDATVAAQTHLVPRLSHRKYIFQNPWIPKACKNEIDYVFFDTKPETDRDYYPFDGPEN
                     LLKDYDNYSKNKEYELILSRDGYAVFKKRVFPGLCGSRLERS"
     gene            3101..4972
                     /locus_tag="VKL21_03310"
     CDS             3101..4972
                     /locus_tag="VKL21_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44830.1"
                     /translation="MNNIFNVVLLLLVSVLIIIPGASANSGYFSDLQSVYGTTGTSCS
                     TCHTSPPALNSYGNAFAAITTHSSDPKGALNSIGVPPGVPAPPDTIPPETAISGVIES
                     GLYNNNVTITLIGTDNTGGSGVKEAIFSVNGGPATIYATPFVVDMIGQDNVSYWSTDN
                     AGNIELPKIVNFTIISESVIDTIPPETAISGVTQSGLYNGSVTITLNAADNPEGSGVK
                     ETVFSVNGGQTATYTAPFVVDTIGQDSVTYWSTDNAGNIEFQKMVNFIITSESLIDTI
                     PPETIISGVTENGLYSNNVAIALIATDNPEGSGVKETMFNVNGGQTATYTVPFIVDII
                     GKDSVTYWSTDTAGNIEPQKMVNFTITSESIDTVPPITNIWGVIENGSYNNNVTISLT
                     ATDNPGGSGVNETIFSVNGGQTATYTEPFVVDRIGRDNVTYWSIDTAGNIELPKMVNF
                     TFEPSINATREIERKSILPGESTNIIVRITSNANQALTLHENIPEGWNVTATSNDMNG
                     FKNSTHEWVLFNLIPGVPKTITYTLTSPGTISIGTYQINGTIRNDGMVENVSGNESVK
                     IDILEFYRRLGSDPAIIETGDLMEAINDSRNSITPSGFDRPLNAEEIGELVNEWVNT"
     gene            complement(5223..>6895)
                     /locus_tag="VKL21_03315"
     CDS             complement(5223..>6895)
                     /locus_tag="VKL21_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44831.1"
                     /translation="ENVRINATWSLGRIGDKRALSVLRYALKNGSTDLRKHAREAIAR
                     IESNGNNTWRNTQEPDISDTVDIPLIAIEVPSNMDCKYISRVEDDESQTDYENMARFS
                     RNVQIRDTNDSFSEDETRKIVLGLKDDSRGLVSVDILFRYKDNVGIEKKSSSVWLQMS
                     SLDGDHRNDREIERIDSRPKDANRTGESSKRKAKIKHSRKPKPVEYDLPEEIGFDPIY
                     KDHEQTDNSEIEENPDERIFKKRIFTRRSVASEIPREDDQETIKPLKRSRKMPLPDEG
                     ELNLETREIPETSEPKPREVKPEVKPAPIVAPEKVKPKPGETKSEVKPAVQAEKPQVK
                     VEVNPEIKPSVAPAATKPASVSVSVNSAVQLLSDIGMSGMTNAASTVTQLSGEEAESL
                     HSQLRTLPIDEMKDEITGLGDNVVMIGVELHGNGQVGEVKGAMQMYISSQNALEIANE
                     LLCNTPDTAIKEFNDDIISTLKETANIFGGQYISAISEYIEVPLTLKTPTFNEGNSSK
                     IAESVLKDITGKVEFALATNLSLGNNRLGRLIMLVDPKSFDIIIQKLF"
BASE COUNT         2110 a         1329 c         1376 g         2080 t
ORIGIN      
        1 cagttgtgaa aagtgtgtga attactctta ataacctgta ctatcaaaag tagattaaat
       61 ataattacgc ccaatataat acaaataaaa aaataattat aaggagaata atgtttgagg
      121 atatttcaga aaaattaaaa agatttaaga tgcctgaaga gtccctcata aagaagatcg
      181 aacgcatcaa gaagactccg gatgccggaa tttcatctgc ttccgtatct aatccatcct
      241 ctgtttctgc aggttcaccg ccaaatgcgc ctgatatttc tgaacaggtc aagaaagccc
      301 aggaagaaat gacgcataaa attgaagaga tcgtaaaatc aaaaacagaa cagatcaatc
      361 aaagtgtaga agctataaag gaatttgatg ttaaaataaa taagcttgaa ggagcagttt
      421 cgaacactaa aagttcagta ggtgaattta aggacaggct ggataaaatc gatgagagtc
      481 ttcttgaatt attatcattg tatgaagttg tttcaagtac tgtaaatcct tttgtcggag
      541 agaagggaat tattcccaac gagaagttgg accagatgga gaaaagaatt gacatcttga
      601 gccagaaacc ttccgggatc cctgcaaata ttaaagaaga atatgatata aaattcaaag
      661 cgcttgaagg gagccttgaa gaattaacac aattaataga ctcggcgccg ccaaaccagg
      721 aagatatgat caggaacgtc tcggaacagg taatggaaag gatcaaaccc cttatcgaac
      781 agatgaaacc catgatagaa caacacttgc agcagactgc ctcacaaact tcttccggta
      841 cacctggttc agcagatact acggctacac gatacccgga tgaaaatatt aaattacaat
      901 atctggataa taaagcggaa acttcgatca tccttttgaa ctggatcgaa ttcttgctgg
      961 aaaaagtagg acggaacaat ctttccgaag tccttgaata ttatattgac ataggttgga
     1021 taagcgagga tgtctgcgaa aaaatgctgg catatgccaa cggtatagat tattatgtgg
     1081 aaagaccaac atggaaactg cttccggatg accatacgaa atctttgatg ttcattgaac
     1141 agttaaaagg aaggaaactc gaaaaatcca tgctgtcaaa actcgaaagg gatgtggaaa
     1201 aggtcatccg cacgaatgaa atctatgttt cgtgataagt tatgaggaga ggaatttcat
     1261 cgtatattcg agcttggacc tatcaagata ttattcttgc agggttaata tttacataca
     1321 taatattaat gagccgtttg gtgtttgacc attacgatgc attgtatgtt tattctttag
     1381 attttggcgt cttcgaccag gctctctggt cgacctccca gggcgatttt atgtggcaaa
     1441 gccaggtagg tttgggaagt aattggggca tacattttga ccctctgttg ctactaactg
     1501 tccctatata ttatattttt ccagacgggc gtttcctgct tgttatccag actatttttc
     1561 ttgcactcgg cgcgatacca ctatatctta tagctaaaat caaatttgaa tcgaggtgga
     1621 ttccgttagc gtttcctata tcgtatttta tatacccgta tcttcataat gttaatcttt
     1681 ttgattttca cagcgtcatg ttctccccac tactaatcgg gttgacctgg tattcgatcg
     1741 aactaaaaaa tattcggctt tttacagtat ttgctctatt atcgctcttt atcaaagaaa
     1801 atatattcgt gattgtaata ttattatgca tttatgcttt tttcaaaacc gaatataaat
     1861 gggaagccac gattttagct gcaattagta ttatttacgg gattgtcgtg atcaaatatg
     1921 taatcccctt tttttcggga aatacatata tgttcataaa aaataatacc gtttcgggaa
     1981 tcatcacaaa ccctccgata gataactttt tggctatcac acaagagctt ttcaaacctt
     2041 tactatattt accgctgctt aaccccctcc cgtacctgac gggcagtata ttgattatgt
     2101 ggcattccgc ccgagatatt tcactcttcc ttacttatca ttatccagcg gatgttatat
     2161 cgcttgttat gatctctgtt atactcgggg tggcaaatat tccgaattac atcaatttta
     2221 tatccaaaac ttactttaaa caagagcaaa agagcattcc aaaaccaaag ttgatacagt
     2281 tggttatagt cagccttatg ctgatatcga gcatacattc attttataca acgagtcagg
     2341 tcatgaacat gcctgcgttg cagtatcaac agaccgagca cgatagaatt ggaatagaca
     2401 ttgcacagag aataccgcct gatgcaacag tagccgcaca aacacactta gtaccaagat
     2461 tatcacatag aaaatatatc ttccaaaacc cctggattcc taaagcgtgc aaaaatgaaa
     2521 ttgactatgt atttttcgac acaaaaccag aaacagatag agattattat ccttttgatg
     2581 gtcccgaaaa tctcctgaaa gattatgaca actattccaa aaacaaagag tacgagttga
     2641 ttttgtcacg agatggttat gcggttttca aaaaacgggt attcccagga ctttgtggaa
     2701 gtcgcttaga acggtcttga agatagaata aaagcgttat cgtcagtttc tccggttgat
     2761 tttgttggaa gaagcccaaa attattgctt tccaaatcta tctttatgat atagataata
     2821 attatcactt aataataaaa atcgaaaggt ttaaatatgg ttcacaacaa agatatttta
     2881 gttgaataag tgaaaaatga aaaacgagat tatggagata tgtattatcc atagattttt
     2941 tctcctatct cggcccggaa tacggaccat ggagtggctc aggatatata attgcaggat
     3001 tggaagtgaa aaaatatgat cggccaggat tacggagagg taaaagatga taatcaaata
     3061 taaacgaggt ataaatatgg tatataaata taaatggcag atgaacaata tatttaatgt
     3121 tgtgctgctg cttttagtat cggttcttat aattataccc ggggcatcgg caaactcggg
     3181 ctatttttca gatcttcaat ccgtgtatgg taccactggc acatcatgca gtacgtgtca
     3241 tacttctcca cctgccttaa attcctatgg aaatgcattt gcagccataa caacccatag
     3301 ctcagatcca aagggtgcat taaactctat aggagttcct ccgggtgttc ctgcgccgcc
     3361 agatactata ccacctgaaa cggccataag tggtgttatt gagagcggtt tgtataataa
     3421 taatgtcacc atcactttaa ttggcactga taatacaggt ggatcgggtg tcaaggaggc
     3481 gattttcagt gttaatggtg gtccagccac gatatatgcc accccattcg ttgttgatat
     3541 gatcgggcag gataatgtat catactggtc aacagataat gcgggaaata ttgaactccc
     3601 aaagatcgtt aactttacta ttatcagtga atcggtaata gatactattc cacctgaaac
     3661 ggccataagt ggtgttacgc agagcggttt gtataatggt agtgtgacca ttactttgaa
     3721 tgctgcggat aatcctgaag gatcgggtgt caaggaaacg gttttcagtg ttaacggcgg
     3781 ccaaaccgca acatacacag cgccattcgt tgttgatacg atcgggcagg atagtgtaac
     3841 atactggtca acagataatg caggaaatat tgaattccaa aagatggtta attttattat
     3901 taccagtgaa tccttaatag ataccatccc gcctgaaaca attatcagtg gcgttactga
     3961 aaacggtttg tatagcaata atgtggccat tgctttgatt gctacagata atccggaagg
     4021 atcaggtgtc aaggagacga tgttcaacgt taacggtggt caaaccgcaa catacaccgt
     4081 gccattcatt gttgatatca tcgggaagga tagtgtaacg tactggtcaa cagatactgc
     4141 aggaaatatc gagccccaaa agatggttaa ctttactatt acaagtgaat caatagatac
     4201 tgtcccgcct ataacgaaca tatggggtgt tatcgagaat ggttcatata ataataatgt
     4261 gacaattagt ttaactgcaa cagataatcc gggaggatcg ggtgttaatg agacgatatt
     4321 cagcgttaac ggcggtcaaa ccgcaacata caccgagcca ttcgttgttg atagaatcgg
     4381 gcgggataat gtaacatact ggtcaataga tactgcagga aatattgaac tcccaaagat
     4441 ggtcaacttt actttcgagc catcaataaa cgcaacacgt gaaatagaaa gaaaatctat
     4501 tctcccgggt gaatccacaa atatcatagt caggatcaca agtaatgcaa accaggcatt
     4561 aactcttcat gagaacatcc ctgaaggatg gaatgtaaca gcgacatcta atgatatgaa
     4621 tggtttcaag aatagtacac atgaatgggt attgttcaat ttaataccgg gagtcccgaa
     4681 gaccatcacc tatacattga catcaccagg aactatttcc attggaactt accagattaa
     4741 tggaacaata aggaatgatg gaatggtgga aaatgtgtca ggaaatgaaa gtgttaagat
     4801 cgatattctg gaattctatc gaagactcgg tagcgatcct gctattatcg agaccgggga
     4861 tctaatggaa gctatcaatg actccagaaa cagcataacg ccttcgggat ttgatcggcc
     4921 attgaatgca gaggagatag gtgaactggt taacgaatgg gttaatactt gaaagaattg
     4981 aatacaaaat ttaaaggagg tgagttatga aaattgtgaa aataaggaaa atcaagaaag
     5041 gtgcctgcaa aagtcattcc tcatgtactt gaaattctcc ccgcctaaag gcgaaagatt
     5101 cctggttcgg ggtgttgccc caccccacaa tttatatttt tctgagatga acacaagctc
     5161 ttgcttacat attatggtat gtaaggtggt ccgtgtattt cactggcata ttttagtaaa
     5221 aactagaaga gcttttgtat tattatatca aaggattttg gatcaaccag cattatcaaa
     5281 cgtcccagcc tgttatttcc caatgataaa ttagttgcga gggcaaactc tactttacct
     5341 gtaatatctt tcaatacaga ttcggcgatc tttgaagaat tgccttcatt gaatgttgga
     5401 gtcttcaatg ttaatggcac ctcgatatat tcggatatgg cactgatata ttgacctccg
     5461 aaaatatttg cagtttcttt taatgttgaa ataatatcat cgttgaattc ctttatcgcc
     5521 gtatcaggag tattgcatag gagttcatta gcgatttcaa gggcgttttg gcttgaaata
     5581 tacatttgca ttgccccttt cacttctccc acctggccat tgccatggag ttccacaccg
     5641 atcatcacta cgttatcgcc aagacctgtt atttcatcct tcatttcatc gatgggaagg
     5701 gtacgcagtt gagaatggag tgattccgct tcctctcctg ataactgggt aacagttgaa
     5761 gccgcatttg tcatacccga cataccgata tcgctcaaaa gctgaacggc cgaattcaca
     5821 gacacagata cgcttgcagg ttttgtagcc gcaggtgcca cagatggctt tatttccgga
     5881 tttacttcaa ccttaacctg aggtttctca gcctgtacag caggtttaac ctcagacttt
     5941 gtttctcctg gtttaggctt taccttctcc ggtgcaacga tcggggcagg tttaacttcg
     6001 ggtttgactt ctctgggttt tggttcggat gtttctggta tttcccgggt ttcaaggttc
     6061 aattcaccct catcaggaag tggcatcttc cgtgatcttt taaggggttt tattgtttcc
     6121 tgatcatctt cccggggtat ttcactggca acagatcgtc tggtaaaaat tctcttttta
     6181 aatatcctct catccgggtt ctcttcaatc tcagaattgt ctgtttgctc atgatcttta
     6241 tagatcgggt caaatccgat ctcctcaggt aaatcgtatt cgactggctt tggctttctc
     6301 gaatgtttta ttttggcttt tctcttactg ctttcaccgg ttctatttgc atcttttggc
     6361 ctggaatcga tcctttctat ttcccgatca ttcctgtgat ctccgtcaag acttgacatt
     6421 tgcagccaga cggaacttga ctttttttcg atacccacat tatctttata cctgaataaa
     6481 atatcgactg ataccagacc cctggaatcg tcttttaatc ccaatacgat ctttcgtgtt
     6541 tcatcttctg aaaatgaatc gtttgtatct cttatctgga cattccttga gaatctggcc
     6601 atattttcat aatctgtctg ggattcatca tcttctactc tggatatata tttgcagtcc
     6661 atatttgaag gaacttcaat tgcaattaaa ggaatatcca cagtatcaga aatgtcaggc
     6721 tcttgagtgt tgcgccaagt attatttcca ttggattcta tcctggctat tgcctccctg
     6781 gcatgttttc gaagatctgt tgaaccattc ttcaaggcat atctcaatac tgataaagcg
     6841 cgtttatcac cgattcttcc cagtgaccag gtagcattta tccgtacatt ttcaa
//
LOCUS       DATGBN010000147         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_166364, whole genome shotgun sequence.
ACCESSION   DATGBN010000147 DATGBN010000000
VERSION     DATGBN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166364"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VKL21_03320"
     CDS             complement(<1..401)
                     /locus_tag="VKL21_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HMB44832.1"
                     /translation="MRLAPELKSKIDALWDKFWSGGLSNPLSSIEQMSYLIFMKRLED
                     MDVAEQKKAHAKKQPYKSVFEGQEDCRWSNWKHYPAEKMLTHVRDIVFPFIKNIHDGE
                     KTLFSQHMKDAVFIIPKPSLLQEAVAILDDL"
     gene            complement(545..1057)
                     /locus_tag="VKL21_03325"
     CDS             complement(545..1057)
                     /locus_tag="VKL21_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44833.1"
                     /translation="MKKESDLHRQIREDLLDKLGDICGRGDIEEYYWSHDVAGDIRFY
                     NHACEKRIFLAQPDIVVIDKNGRVLVIGIELTNTPKHLFGVVSTINSSSIGKFSRKNI
                     DLSSKSMLLVLNFDDIPKKGSCKQTQIDSLRCLIEESTDFEFFDIISDTEAIDKIYCW
                     LGDENDVVEI"
     gene            complement(1054..>1616)
                     /locus_tag="VKL21_03330"
     CDS             complement(1054..>1616)
                     /locus_tag="VKL21_03330"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HMB44834.1"
                     /translation="GTEINDIRYGTGSPPEYQKEGIPFIRATNIKKGTVQKDGLVYIS
                     KEEANKIKKCEVKTGDLILVRSGVNTGDCARIPSEYDGAYAGYDLVIQMPYPKNYYYN
                     FIINSDYGKSVIKPLSRRAGQPHINADQVNSLQFQVPPLPLQQEFARIVEKVEAMRQS
                     QNQSKQQIEDLFSALMQKAFRGELVA"
BASE COUNT          437 a          338 c          303 g          538 t
ORIGIN      
        1 ttcaggtcat ccaggatggc gacagcttcc tgaaggagcg agggcttggg gataatgaaa
       61 accgcatcct tcatgtgctg ggagaagagc gttttttcgc catcgtgtat gttttttatg
      121 aatgggaaca cgatgtctct aacatgagtg agcatctttt cagctggata atgtttccag
      181 tttgaccacc tgcaatcctc ctgtccctca aaaacggatt tgtatggctg ctttttcgca
      241 tgcgccttct tttgctcggc cacatccata tcctcaaggc gcttcatgaa aataaggtat
      301 gacatctgct ctattgatga aaggggattg gataagccgc cgctccagaa tttgtcccag
      361 agggcgtcta ttttggattt aagttcgggt gcgagtcgca tgttatctgt tctccatttg
      421 atttacccct acaaattgag attttttcat gattttagca aaatgtaggg gtaagaacat
      481 tcgataaaaa tcattccctt ccattgtatg cgtattggtt tctaaattag catatattaa
      541 aatatcatat ttcaaccaca tcattctcat cacctaacca gcagtatatc ttatcaattg
      601 cttcagtatc agaaataata tcaaaaaatt caaaatctgt cgattcttca atcaaacatc
      661 ttaaagaatc tatttgggtt tgtttgcaag aacctttttt tggtatgtca tcaaaattaa
      721 gaaccaacag catggactta cttgaaaggt ctatattttt tctagaaaat ttgcctatac
      781 ttgatgaatt tatggttgaa acgactccaa ataagtgctt aggggtattt gttagttcta
      841 ttccaataac gaggacacgc ccgttcttat caataaccac aatatccggc tgggcaagga
      901 atatcctctt ttcacaggca tgattgtaaa acctgatgtc gccggcaacg tcatgagacc
      961 aataatattc ttcaatgtcg cctctgccgc atatatcccc taatttatct aaaaggtctt
     1021 ctcttatctg cctgtgcaag tctgattcct ttttcatgct accagttccc ccctgaacgc
     1081 cttctgcatc agggcgctga acaagtcctc tatctgctgc tttgattgat tctgggattg
     1141 gcgcatggct tcgactttct ccacgatgcg ggcgaattct tgctggaggg ggaggggagg
     1201 aacctgaaat tgcagagaat taacttgatc agcatttata tgaggttgac ctgcccttcg
     1261 agacaaaggt tttattactg attttccata atccgaatta ataataaaat tataataata
     1321 atttttagga tacggcattt gtattacaag atcgtatccc gcataagctc catcatattc
     1381 agaaggaatt ctggcgcagt caccagtatt gacaccactt ctgacaagaa ttaaatcacc
     1441 agttttaact tcacatttct taattttgtt agcttcttct ttcgaaatat atacaagacc
     1501 atctttttgt actgttccct tcttgatgtt tgttgctctg atgaatggaa taccttcttt
     1561 ttggtattct ggaggagatc cagttccata tcgaatatca ttgatctctg ttccca
//
LOCUS       DATGBN010000148         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_127849, whole genome shotgun sequence.
ACCESSION   DATGBN010000148 DATGBN010000000
VERSION     DATGBN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127849"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VKL21_03335"
     CDS             <1..315
                     /locus_tag="VKL21_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44835.1"
                     /translation="SMYKSWIDALQKMSEKAKELSTQTSDPEAYKEFYNLWVKMYEKA
                     FDSFFEDIPTVGGPMKEVMEPIKVMAKMYADTFARISKMWVKSDFGSASAYPGKNKKG
                     KI"
     gene            327..791
                     /locus_tag="VKL21_03340"
     CDS             327..791
                     /locus_tag="VKL21_03340"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HMB44836.1"
                     /translation="MEKSSFESTQEFYGLWLKTYKATLGRLVEMPTMGPAREKSEKMM
                     KGFSTFINLYAAGMDTSSNLQSVFMEAMRKVQEKTATDMEGEISPEKYKDFYNIWIET
                     YSETFKEFSKSEHFVADLGKLNSNLMDFQIYNREMLEENYLKPMNLPTKTGR"
     gene            814..1980
                     /gene="phaC"
                     /locus_tag="VKL21_03345"
     CDS             814..1980
                     /gene="phaC"
                     /locus_tag="VKL21_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HMB44837.1"
                     /translation="MNGLFLLEYFEKFKHGMELFLKPPYFSVGTTPSEMIFSEDKMKL
                     IHYIPTVEKTHPVPILIVYALVNRYYILDLQPDKSVIKKLLDEGFDVYVIDWGYPSGV
                     DRYLTLDDYVNGYINNVVDKIRERSGLDKITLLGVCQGGTFSVMYAAQHPDKVKNLVT
                     LVAPVDFDTDKGLLHLWAKSLDVDKIVDYYGIVPGDFLNAGFLLTDPFRLMIDKYVGI
                     FDRIECEPNDTACQLRNQETIKNFLRMEKWIFDSPDQAGETFRQFMKDCYQKNLLIKN
                     EMMLNGKKINLKNITMPLLNVMAEFDHLVPIDASKPLTEAVSSSDKETLVFPTGHIGI
                     FVGSKSQKEVCPRIAEWLKPRSLLDRTEEEKPESSKEKSREQKRKNRRSRVKEK"
     gene            1977..2714
                     /gene="fabG"
                     /locus_tag="VKL21_03350"
     CDS             1977..2714
                     /gene="fabG"
                     /locus_tag="VKL21_03350"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMB44838.1"
                     /translation="MRLKNKVVLITGAGSGIGKETALLFAREGAKVVVSDMNESTGEE
                     TVAQIKKNGEGFFAKLDVSNREQAKQVVKITLEKYGKIDVLINNAGIVQNAFLSKMTE
                     EQWDKVINVNLKGVFNCTQAVVEVMMNQGNGVIINTSSIVGLNGNVGQVNYAATKAGL
                     IGITKTLAKELGRKGIRVNAVAPGFITTPMTSNVPDKILEMMKEKTPLRRLGEPKDIA
                     NAYLYLASDDANFVNGAVLCVDGGLII"
     gene            2731..>3492
                     /locus_tag="VKL21_03355"
     CDS             2731..>3492
                     /locus_tag="VKL21_03355"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMB44839.1"
                     /translation="MNNVVIVSATRTAVGKFGGSLKDFNPGQLGSVVLKEALKRANVE
                     SDEVDEVIMGNVLSAGHGQNVARQAAIGAGIPNEVPSYCVNKVCGSGLKSVVLGAQSI
                     MLSDADIVLAGGMESMSTAPYALKKNRWGAKMGNDEVVDLMIHDGLWDIFNNYHMGVT
                     AENLAEKYRITREEQDEFSAISQNKAETAIKGGKFKDEIVPVGIPQPKGEPVLFDTDE
                     FPRFGTTKEILAKLKPAFKKEGTVTAGNASGINDGA"
BASE COUNT         1176 a          617 c          832 g          867 t
ORIGIN      
        1 agcatgtaca agtcctggat cgatgcactt caaaaaatgt cagaaaaggc taaggagcta
       61 tcgacacaaa cctctgatcc agaggcatat aaagaattct ataacctatg ggtaaagatg
      121 tatgaaaagg cttttgatag cttctttgag gacataccga cagtaggagg ccctatgaaa
      181 gaggtcatgg agcctataaa ggtcatggct aagatgtacg ctgacacgtt tgccaggata
      241 tcaaagatgt gggtaaaatc tgatttcggt tcagcgtcag catatccggg aaagaataaa
      301 aaaggaaaga tctagacaga ttaaatatgg aaaaatcaag ttttgaatcc acccaggaat
      361 tttatggcct gtggttaaag acatacaaag ccacattggg caggctcgtt gagatgccta
      421 ccatgggtcc ggcacgtgag aaatctgaaa aaatgatgaa ggggttctca acctttatca
      481 acctttatgc cgcagggatg gataccagtt ccaatttaca gagtgtattc atggaagcaa
      541 tgcgcaaagt gcaggaaaag actgcaaccg atatggaagg ggaaatcagt cctgagaaat
      601 ataaggattt ttacaatatc tggatcgaga cttatagcga gaccttcaag gaattctcaa
      661 aatcagaaca ctttgtggct gacctgggga aattgaattc gaatttaatg gattttcaaa
      721 tatacaacag ggaaatgctt gaagaaaact atctaaaacc catgaatctc cctacaaaga
      781 cgggaagata aaagatagag ttttggagat gccatgaacg gacttttttt attagaatat
      841 tttgagaaat tcaaacacgg gatggaacta ttcctgaagc ccccctattt ttcagttgga
      901 acaacgcctt cagagatgat atttagtgag gataagatga aactcatcca ttatattccg
      961 accgttgaaa aaactcatcc agttccaata ctcatagttt acgcacttgt gaacaggtac
     1021 tacattctgg acctacagcc agataagagc gttataaaga agcttctgga tgaaggcttt
     1081 gatgtatatg tcattgattg gggatatcct tcaggagtcg acagatacct cacgcttgac
     1141 gactatgtaa acggttatat aaacaatgtt gtggataaaa ttagagagcg gtctggattg
     1201 gataagatca ctctccttgg agtatgccag ggtgggactt tttctgtgat gtatgccgca
     1261 cagcatcctg ataaagtaaa aaacctggtt actttagtgg ctccagtgga cttcgatact
     1321 gataaaggac ttcttcatct ctgggcaaaa agcttagacg tggataaaat cgtggactat
     1381 tatggaatag ttcccggaga tttcctgaat gccggattcc tgctcacaga cccgttcagg
     1441 cttatgatcg acaagtatgt gggtattttt gatcgaatcg aatgtgaacc gaacgataca
     1501 gcctgccagc tcagaaatca agaaactatc aagaatttcc ttagaatgga aaaatggata
     1561 tttgacagtc ctgaccaggc cggagaaacc ttcaggcagt ttatgaaaga ctgctatcag
     1621 aagaaccttc tcattaaaaa tgagatgatg ctcaatggta agaagatcaa tttaaagaat
     1681 attacaatgc ctctgcttaa tgtaatggcg gaattcgacc acctagttcc aattgatgca
     1741 agtaaacccc tgactgaggc ggtatcaagt tcggataaag aaactctggt tttccccacc
     1801 ggacacatag gtatctttgt gggctccaaa tcccagaaag aggtctgtcc caggatcgca
     1861 gagtggttaa aacccaggtc tcttcttgat agaacagaag aagagaaacc tgaatcttcc
     1921 aaagaaaaat cacgagaaca aaaaagaaag aatcgcagat caagggtgaa agaaaaatga
     1981 gacttaaaaa taaagtagta ctaattacag gagcaggaag tggtattggc aaagaaactg
     2041 cgctcctgtt tgcaagagaa ggagccaaag ttgttgtctc cgatatgaat gaaagtactg
     2101 gcgaggagac agtagcacag atcaagaaaa atggagaggg attttttgcc aaattggatg
     2161 tatccaatag agaacaggca aaacaggtgg ttaaaatcac tcttgaaaaa tatggtaaaa
     2221 tagacgtttt gataaataat gcaggcattg ttcagaatgc ctttttatca aaaatgaccg
     2281 aggaacagtg ggataaggtt atcaacgtca atcttaaagg agtttttaat tgcacccagg
     2341 ctgttgttga agtcatgatg aaccagggta atggggtaat tatcaataca tcttcgattg
     2401 taggattaaa tggcaatgtg ggccaggtca attatgctgc aaccaaagcc ggtctaattg
     2461 gtattacaaa aactctggca aaagaactgg gaagaaaagg gataagggtc aatgcagttg
     2521 cgcctggatt tattacgact ccgatgacct caaacgtacc tgacaaaatt cttgaaatga
     2581 tgaaagaaaa aacaccattg cgcagacttg gtgaaccaaa agatattgca aatgcatatt
     2641 tgtatcttgc ttcagatgat gcaaactttg taaatggagc tgtgctctgt gtggatggcg
     2701 gattaataat ataataaaaa ggtgaaaaca atgaataatg tagttattgt atcggcaact
     2761 agaacagctg tcggaaaatt cgggggaagt ctgaaggatt ttaatcccgg ccaacttggt
     2821 tctgtggtat tgaaagaagc attgaaaaga gcaaatgttg aatctgatga agttgatgag
     2881 gtaatcatgg gaaatgttct ttcagcgggt cacggccaga atgtggcacg acaggctgca
     2941 attggagcgg gaattccaaa tgaggtaccc tcctattgcg tgaacaaggt ttgcggctca
     3001 ggcttgaaat ccgttgttct gggagcacaa tccatcatgc tgagtgatgc ggatattgtg
     3061 ttggctggcg ggatggaatc catgtcaacc gctccatacg cgctgaaaaa aaaccgctgg
     3121 ggagcaaaga tgggcaacga tgaagtcgtg gatttgatga tacatgatgg actgtgggat
     3181 atttttaaca attaccatat gggcgtcaca gctgagaact tagcagaaaa atacagaata
     3241 acgcgggaag aacaggatga attctcagca atcagccaga ataaggcaga aacagcgatc
     3301 aagggaggaa agttcaagga tgagatcgtt ccagtgggaa ttccccagcc aaaaggcgag
     3361 cctgttctct ttgatacaga tgaattcccc aggttcggca caacaaaaga gatccttgca
     3421 aaactaaaac ctgcattcaa gaaagaaggg acagtaactg cgggcaatgc ttcaggaatc
     3481 aacgatggag ca
//
LOCUS       DATGBN010000149         5544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_49816, whole genome shotgun sequence.
ACCESSION   DATGBN010000149 DATGBN010000000
VERSION     DATGBN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49816"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..390)
                     /locus_tag="VKL21_03360"
     CDS             complement(103..390)
                     /locus_tag="VKL21_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMB44840.1"
                     /translation="MYLSKSLPEVLVPKYYKSVDNLIEDAFRALLNLKPGLKVEMAIE
                     LYLREEISLSRGAEMAGMDMESFKDILKVRGLKVSSYIGSKEEIESGLRLI"
     gene            complement(424..660)
                     /locus_tag="VKL21_03365"
     CDS             complement(424..660)
                     /locus_tag="VKL21_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44841.1"
                     /translation="MIQIKENHLRIKEVEIHCSYEVERASTQNPVTHGLGVLLAILRQ
                     IEFKKPLYILTLPGIIMVLLCLGIGLFYGMKLKV"
     gene            complement(657..1145)
                     /locus_tag="VKL21_03370"
     CDS             complement(657..1145)
                     /locus_tag="VKL21_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB44842.1"
                     /translation="MITEGNILNTIKEKGLEVLEIPVASNGSNDSGSLSTERKFTIAA
                     MPAYNEERSIAKMVLQCRKYADMVVVFDDGSADSTADIAKAMGAHVIRHPQNRGYGGV
                     LQTCFETARDLAADCMVILDSDGQHDPADIPKLLGPLNGGCDVVIGSRFINGNGKKAL
                     KY"
     gene            complement(1280..1873)
                     /locus_tag="VKL21_03375"
     CDS             complement(1280..1873)
                     /locus_tag="VKL21_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB44843.1"
                     /translation="MSFPIKIIDEDFIYINFEDPEMIGSGIKDMLDAVNVHEEFYGKI
                     PEYIFLDEVQNIDNWEKAVRGLYETKNYYLFISGSSSKLLSKEIATSLRGRTLTYSIL
                     PLSFREFLGFNDIKIKPLLSTSDENQIKNNLRKYMEFGGFPDIVFDNQITERFFKEYL
                     DLVLFRDIIERYNIKNIFIIKFLIRSMLASFSKQVWN"
     gene            complement(1852..2142)
                     /locus_tag="VKL21_03380"
     CDS             complement(1852..2142)
                     /locus_tag="VKL21_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44844.1"
                     /translation="MIQKSLSLLLPYINSVNCLGVLYSSCPMKSRLMIYLKPLYLNSA
                     GVAKPQTPTDAPPLAGFNNILPVAYNHNTPYLHDIDRKPENATIFKLVSPSK"
     gene            complement(2146..2589)
                     /locus_tag="VKL21_03385"
     CDS             complement(2146..2589)
                     /locus_tag="VKL21_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMB44845.1"
                     /translation="MEKPDRERIFAKLDEMTGYVEELKEMLPEEEEYSQNLIKRRACE
                     KTIEVAIESLIDVSAMMVSAQRFGLPASEESILDILVEKKVLGKELGERLKDMKGFRN
                     ILIHRYAHVDDKIVYQHMTDYLVDFFEFGKAIESYIGKSKCACDE"
     gene            complement(2592..2999)
                     /locus_tag="VKL21_03390"
     CDS             complement(2592..2999)
                     /locus_tag="VKL21_03390"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB44846.1"
                     /translation="MKIMESIVASEHTIPEVIEKMRAIGKDKVRFVVLYGSAAKGKLT
                     KLSDLDLAVFFKGDKSEKFEFRKKILGRVSNEFDIQIFQDLPLYVQKDILSTGKMMYS
                     EDYIETFEIFMKTIRGFEDFKPRLEVYYSGLGV"
     gene            complement(3064..4134)
                     /gene="wecB"
                     /locus_tag="VKL21_03395"
     CDS             complement(3064..4134)
                     /gene="wecB"
                     /locus_tag="VKL21_03395"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMB44847.1"
                     /translation="MTLSIILGTRPEIIKMSPVIRECQRLELDHFILHTGQHYSYNMD
                     RVFFEELRLPDAKYNLEVGSGAQGEQTAKMMTGIEKVLMKEKPEAVLVQGDTNTVLAG
                     ALAAVKLGIKVGHVEAGLRSYDRRMPEEINRVMADHCSDQLFAPTEKCKKTLLGEGIP
                     GSRIHVTGNTIVDAVFQNLKLAKEKNTLEGLDITPNEYFLVTAHRQENVDDPVRFAKL
                     LKGLEKIGNEFNIPVIFPIHPRSKKRVEEFGIRTDGSIRFIDPVEFLDFLNLESGARL
                     VLTDSGGVQEETCILGVPCVTLRDNTERPETLDVGSNMLSGTEPEMMTGCVHRMLEKD
                     KGWINPFGDGKAAERIVKMMRS"
     gene            complement(4100..5068)
                     /locus_tag="VKL21_03400"
     CDS             complement(4100..5068)
                     /locus_tag="VKL21_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB44848.1"
                     /translation="MTVTAILPAFNEEVSIGSAVLHARQHADHVVVIDDGSKDRTSEM
                     AKLAGAEVIRHPKNMGKGMALRTGFEHAEKNGCKIVITMDSDGQHDPDDIRKLVAPIF
                     NGEADIVNGSRYMNGNDENTPFYRRIGQNILDKATNLNAGLQISDTQSGFRAFAWHTL
                     PAFRFKSKGLAIESEMLMDAANAGFRIKEVSISARYDVDCSSENPIKHGLGVLANVIH
                     DMELNRPLYYFTLPGAILSIIGLAMGFSFLKKFYDGGSLYFGPTLLMILFTLIGTFMV
                     FTGIILHTVAKLITENTKKVNISSNSDPIAGPTVKHDSFYNSWNSS"
     gene            complement(5192..>5544)
                     /locus_tag="VKL21_03405"
     CDS             complement(5192..>5544)
                     /locus_tag="VKL21_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HMB44849.1"
                     /translation="FMIINAACSGSASMSVFLVIFMLMSLDLPLPGRKWAFLLFFGII
                     GTFLQNIVRLVLLMLVGYYLGPSAIESGESFTGYILFPLWYAIFAYVYLKQYKIVNSS
                     CDISSSNKYHDYNH"
BASE COUNT         1579 a         1334 c          928 g         1703 t
ORIGIN      
        1 aaccatcggc gttattccga cttttccaaa gagcttaata agaacatcaa agtgtccagc
       61 ttttgcaaac gcactggcaa tatcagtatc aagtattagc aatcagatca accttaatcc
      121 actttcgatc tcttcttttg aaccaatata actgctaacc tttaatcccc tcacttttaa
      181 aatatcctta aagctttcca tatccattcc cgccatctca gcaccccttg aaagtgaaat
      241 ttcctccctc aaataaagtt caatagccat ctcaactttc aaccctggct tcaaatttaa
      301 gagtgctctg aatgcatctt caatgagatt atctacgctc ttgtaatatt taggtaccaa
      361 aacctctgga agactctttg ataaatacat attcatataa taccgtccac actataaata
      421 actctatacc ttcaatttca taccataaaa taatccaata cctaaacaca gaagtaccat
      481 aataattcct ggcaatgtca ggatatacaa tggtttcttg aactcgatct ggcgcagtat
      541 tgccagcagc acccctaatc catgtgtgac aggattctgt gttgaagccc tctcaacctc
      601 ataactgcag tgtatctcta cctcttttat cctgaggtga ttctctttta tctgtatcaa
      661 tatttcagag ccttctttcc atttccattg atgaatcttg accctatcac tacatcacaa
      721 cctccattca aaggcccaag aagctttggt atatcagcag gatcatgctg cccgtcagaa
      781 tcaagaatca ccatacaatc cgcagctaga tctcttgctg tttcaaaaca tgtttgcagc
      841 acgccgccgt agcccctgtt ttgaggatgc ctgataacat gagctcccat agctttggca
      901 atatccgctg ttgaatcagc gctgccatca tcaaaaacta ccaccatatc agcatatttc
      961 ctgcactgta ataccatttt ggcaatggag cgctcttcat tgtaagcagg cattgcagct
     1021 attgtgaatt ttctctccgt gctaagagat ccactatcat tcgaaccatt agatgcaaca
     1081 ggtatttcta atacttccaa ccctttttct tttattgtgt ttagaatatt gccttctgtt
     1141 atcaaacaat acctaaatca ttttttaagg ttatactatt tcagtagcca tttccaaaga
     1201 ggtaaataaa ccaccttttt attccctatg cgctcctccc cttccctgtc ccaggtgatc
     1261 acaagcagat cgctgcattt tagttccata cttgctttga gaaggaagca agcatggatc
     1321 ttatgagaaa ttttatgatg aaaatatttt ttatattata tctttcaata atatccctga
     1381 aaagtacaag atcaagatat tctttaaaaa acctttctgt gatttgattg tcgaacacta
     1441 tgtcaggaaa accaccaaat tccatatatt tgcggagatt atttttgatc tggttctcat
     1501 ctgatgttga aagtagcggc ttgatcttaa tatcattaaa gcccaaaaat tccctgaatg
     1561 ataacggaag aatagaatac gtcaatgtcc tccctcgtaa cgaggttgct atttctttag
     1621 agagcaattt tgaagatgaa cctgaaatga acaggtaata gttttttgtt tcatataatc
     1681 cccttacagc tttctcccaa ttatcaatat tttgaacctc gtccagaaat atgtattcgg
     1741 gaattttgcc ataaaactcc tcgtgaacgt tcactgcatc aagcatgtct ttgatgcctg
     1801 agcctatcat ttcaggatcc tcaaaattga tgtagatgaa atcttcatca attattttga
     1861 tggggaaact aatttaaaaa tggtcgcatt ctctggcttc ctgtcgatat cgtgcaagta
     1921 tggagtgttg tgattatatg caaccggtaa tatattgtta aaccccgcca gcggcggcgc
     1981 atctgttggg gtctggggtt tcgcaacccc agcgctgtta agataaagtg gttttaaata
     2041 tatcataagc ctggatttca tagggcagga agaatacaga acacccaggc aatttacgga
     2101 gttaatatac ggtaaaagca aagaaagcga tttctgaatc acatttcact catcgcaggc
     2161 acattttgat ttcccaatat atgactcaat tgcttttcca aactcaaaaa aatcaaccag
     2221 gtaatcagtc atgtgttgat atacaatttt atcatctaca tgcgcgtagc ggtgtatcaa
     2281 tatatttcta aatcctttca tgtcttttag cctttcgcct aattccttac ccagaacttt
     2341 tttttcaacc agtatgtcca gaatgctctc ttcacttgca ggtagaccga accgttgagc
     2401 agaaaccatc attgccgaca catctatcag tgattcgatc gcgacctcaa tagttttctc
     2461 gcaggctctc cttttgatta aattctgtga atactcctct tcttcaggaa gcatttcttt
     2521 cagttcttcg acataccctg tcatctcatc gagtttggcg aaaatacgtt ctcgatctgg
     2581 tttctccaag tttacacccc taaaccagaa taatatactt ctaagcgtgg cttgaaatcc
     2641 tcaaatcccc tgatagtctt cataaatatt tcgaatgttt ctatatagtc ttcagaatac
     2701 atcattttac ctgtggaaag gatatctttc tgcacataca gcggtagatc ctggaaaatt
     2761 tgaatatcga attcattact caccctgcca agaattttct tcctgaattc aaatttttca
     2821 ctcttatcac ccttgaaaaa aacagcaaga tcaagatcag ataactttgt aagtttcccc
     2881 tttgcagcag agccatataa taccacgaac cttactttat ctttgccaat agctctcatc
     2941 ttctcaatta cttcagggat agtatgttcc gaagctacta ttgattccat tattttcata
     3001 aaacacttat aacaaatcgc ttaaaatata agtcctttgt tacgaaatac acagatatcc
     3061 cattcatgac ctcatcatct tcacgatcct ctctgccgct tttccatccc cgaatggatt
     3121 tatccatcct ttatccttct caagcatcct gtgaacgcat ccggtcatca tctcaggctc
     3181 agtcccggaa agcatattcg agcccacatc aagcgtctca ggtctttccg tattatccct
     3241 cagcgtcacg catggcacac ccaggatgca tgtttcttcc tgcacaccgc cagaatccgt
     3301 aaggacaagc cgcgctccgc tttcaaggtt aagaaagtca aggaactcca caggatcgat
     3361 gaacctgatg cttccgtcgg ttctaatccc gaactcttcc accctctttt tcgagcgcgg
     3421 atggatggga aagataacag ggatattgaa ctcattaccg atcttctcaa gacccttcaa
     3481 aagctttgcg aacctcaccg gatcatccac attctcctgc ctgtgcgccg taacgaggaa
     3541 atattcattt ggcgtgatat caagcccctc aagcgtgttc ttctcttttg caagcttcag
     3601 gttctgaaaa acagcatcca caatggtatt cccggtgaca tggatcctgc tcccgggtat
     3661 cccttctcca aggagtgttt tcttacattt ctcagtaggt gcaaaaagtt gatcagaaca
     3721 gtgatcagcc atcaccctgt taatctcctc aggcatcctt ctatcataac ttcgaaggcc
     3781 tgcctcaaca tggcccacct taatccccag tttaacagca gcaagcgctc ctgcaagcac
     3841 agtgttagta tccccctgca caagcaccgc ttcaggcttt tctttcataa gcactttttc
     3901 tatccctgtc atcatcttag cagtctgttc cccctgtgcc cctgagccca cttcaagatt
     3961 atatttagca tcaggcagcc tcaactcttc aaagaatacc ctgtccatat tatatgaata
     4021 atgctgtccc gtatgcaata tgaaatgatc aagctccaat cgctggcatt cccttatcac
     4081 cggggacatc ttgattattt caggacgagt tccaagaatt atagaaagag tcatgtttta
     4141 ccgtcggtcc tgcaattgga tcagagtttg atgatatatt aacttttttc gtgttttcag
     4201 tgatcagttt ggccacagta tgcaggatta tcccagtgaa caccataaac gtcccgatga
     4261 gagtgaaaag gatcatcaac agcgttggtc cgaaatataa gctccctcca tcatagaatt
     4321 ttttcaggaa actaaaaccc atcgccaatc cgataatact taatatcgct ccgggtaatg
     4381 tgaaataata tagaggtctg ttaagttcca tatcatgaat aacattagcc agaactccta
     4441 atccatgttt gatgggattc tcagatgagc agtcaacatc atatctcgca cttatgctca
     4501 cttcttttat acggaatccg gcatttgcag catccataag catctcactc tcgatggcaa
     4561 ggcctttcga tttgaacctg aaagcaggta aagtatgcca ggcaaatgct cgaaaaccgc
     4621 tctgggtatc actgatttgg agcccagcat taaggtttgt tgccttatca agaatgtttt
     4681 gccctattcg tcgataaaaa ggagtgttct catcatttcc attcatatac ctgctgccat
     4741 tcacaatatc cgcttctcca ttgaatattg gagcaacaag ctttcggata tcatcagggt
     4801 catgctggcc atcggaatcc atagttataa ctatcttgca gccattcttt tctgcgtgct
     4861 caaaacctgt cctgagtgcc atgccttttc ccatattctt aggatgacgg atcacttctg
     4921 cgccagcaag ttttgccatc tcggaagtcc tgtccttgct gccgtcgtct attacaacta
     4981 catggtcagc atgctgtctt gcatgaagca ccgcgcttcc tatagaaact tcttcgttaa
     5041 atgctggtaa gatcgctgtt acggtcatat tatatgaatc accttttttt cacgacattt
     5101 gatccttttt tatttatggt gtctgattta aggtgtaatt accccgaccc cctaatatga
     5161 tttcaaggcg tttaaatcta ccgataaata tttaatgatt ataatcatga tacttatttg
     5221 atgatgagat atcacatgag ctgtttacta tcttgtattg cttcagataa acataagcaa
     5281 agattgcata ccagagagga aataggatgt atcccgtgaa actctccccg ctctcaatcg
     5341 cagaaggacc taaataatat ccaacaagca tcagcagcac aaggcgcaca atgttttgca
     5401 agaacgtacc aataatgcca aaaaatagga ggaatgccca tttgcgcccc gggaggggaa
     5461 gatcaaggga cataagcatg aagatcacca ggaatactga catcgaggct gaaccagagc
     5521 aggctgcatt tatgatcata aaaa
//
LOCUS       DATGBN010000150         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_476910, whole genome shotgun sequence.
ACCESSION   DATGBN010000150 DATGBN010000000
VERSION     DATGBN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476910"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VKL21_03410"
     CDS             <1..412
                     /locus_tag="VKL21_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44850.1"
                     /translation="YGANFGLSAKEIEQIARHKIVLLNMGNVNSHIISKAKKILKYIN
                     IPAVIACEYPIDFEDFAKAGIKTKFVKPLKPETEGTVVAIVSGITRGETCSRIKLNEL
                     AKAIRQILSEKIESTHAVRSDLLISEGLMASEGE"
     gene            420..632
                     /locus_tag="VKL21_03415"
     CDS             420..632
                     /locus_tag="VKL21_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HMB44851.1"
                     /translation="MKCYVCAKMGKDTDAVAVCIVCGMGVCMEHAIYETTPVWKGDYP
                     VRLEKDVENMKRIMCPPCHEALKENW"
     gene            638..859
                     /locus_tag="VKL21_03420"
     CDS             638..859
                     /locus_tag="VKL21_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HMB44852.1"
                     /translation="MLKCYQCTESGTVEESVSICIMCGKGLCMEHAKRIDLPIWEGGY
                     PAPVKMLKKGLPRFVCSDCGNILLPGSCE"
     gene            866..1765
                     /locus_tag="VKL21_03425"
     CDS             866..1765
                     /locus_tag="VKL21_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HMB44853.1"
                     /translation="MFEVRFHGRGGQGVVMAAQTLAEAAVNEGNYAVAFPFFGAERRG
                     APVLAFTRIDSKKIFSKTQVYAPDCVVVLDDSLLDTIDVVAGLKPQGLAIINSKEKPE
                     DIDLGKIINTATVNATSIALEILKAPITNTAILGAFAKATGIIKIESLEESIKRKFGE
                     KLGAKVGERNALAARTAYERTITGKSRGVKKLEARKQWLPTHQEIPIGGSLIEGKTDA
                     GLIGPGSFIENKVSGWATYRPLRDKAKCTMCLLCWFYCPEGTIVRISDRGDLMTNYDY
                     CKGCGVCANECPVDAIKMVRARV"
     gene            1762..2934
                     /gene="porA"
                     /locus_tag="VKL21_03430"
     CDS             1762..2934
                     /gene="porA"
                     /locus_tag="VKL21_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HMB44854.1"
                     /translation="MKKVITGDHAVAMGAKLCRVEVIPAFPITPQTLIIEHIADFVND
                     GELDAKFLTMESEHSVMSAAAGASAAGARVFTATSSQGLAFMHEMLYATAPLRLPIVM
                     ANASRTLGGPPGIWCEYNDSMGARDSGWLQLYVEDNQEALDMVIQSFRIAEDKRVLLP
                     VMPCLDGFVLTHTVEPVDVPEQEEVDSFLLPYEPDIILDPARPAMIGTFMPSEYIMEL
                     RRQTTGAFESAKEVIQQVNNEFAQKFGRDYGGLIDTYMMEDASIALITMGTVTSTSRE
                     VVDELRQKGEKVGIIKLRFFRPFPSYELKKAVSHISALGVFDRSISYHGGGPVYNEVL
                     SALYGMTIPVINHLAGIGGRDVTREQIMKMFELTQKAGKGEKVNDIYWHNTRGETV"
     gene            2931..3893
                     /locus_tag="VKL21_03435"
     CDS             2931..3893
                     /locus_tag="VKL21_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMB44855.1"
                     /translation="MITIKELSDVNLFTAGHTACPGCGVAMSIRNAIRVLGKDTVVYV
                     PASCAVVFGTTFPYGAWKVPFFHTAFENTGACLSGMQAAFEKKGKKVTVVGFAGDGGT
                     YDIGLQSMSGAAERNDDVLYICLDNEAYMNTGIQRSGGTPFGAWTTTTPVGKVVQGNR
                     RFKKNLGDIVAAHEIPYYATLSIGQPLDFIRKVEKAKNIRGFRFLHMFTPCIPGWKMD
                     PAKTVEITKRAVESGMWTLYEIENGEKRITHKPGKMKSVKDYLMMQGRFRHMSEEDIK
                     KLQMWVCKKWNLHYKEKGAPEVCEILPPEEHHAVTEEHREMHGP"
BASE COUNT         1156 a          769 c         1058 g          937 t
ORIGIN      
        1 ttatggagcc aattttggac tgagcgcaaa agagatcgaa cagatagccc ggcataagat
       61 agtgctatta aatatgggaa atgtcaattc ccatataatt tcaaaggcaa agaagatctt
      121 gaaatatata aatatccccg cagtgatcgc ctgtgaatat cccattgatt ttgaggattt
      181 cgcaaaagct ggcataaaga caaaattcgt aaaaccttta aaacctgaaa cagaaggcac
      241 tgtggttgcg atcgtaagcg gcataacaag gggtgagacc tgctcaagga tcaaattgaa
      301 tgaactggca aaggctatac gtcagatcct gagtgagaaa atagaatcaa cccatgcggt
      361 cagaagcgac ctgttgataa gtgaaggatt aatggcaagt gaaggagagt gaaagtaata
      421 tgaaatgtta cgtttgcgcc aaaatgggaa aagatactga tgcggtagcg gtctgtatcg
      481 tgtgcggtat gggtgtttgc atggaacatg caatatatga gaccactccc gtatggaaag
      541 gagattatcc ggtacggctt gaaaaggatg ttgagaacat gaagcgtata atgtgccctc
      601 cgtgtcatga ggccttaaag gagaactggt gatcgtcatg ttgaaatgtt atcaatgcac
      661 agaaagcggg actgtggaag aatccgtatc gatctgcata atgtgcggca aagggctttg
      721 tatggagcat gcaaaaagga tcgaccttcc tatatgggaa ggaggatatc ccgctcccgt
      781 caagatgctt aaaaaaggtc ttccgagatt tgtctgcagt gattgcggga acatcctgtt
      841 accaggatcc tgtgaataaa aaagcatgtt cgaagtaagg ttccatggcc gggggggaca
      901 gggagtggtc atggccgctc agaccctggc agaggctgcg gtaaatgaag gaaattatgc
      961 tgtagcattt ccttttttcg gcgctgaaag acgtggggct ccggttcttg ccttcacgcg
     1021 tatagattca aaaaaaatat tcagtaaaac gcaggtgtat gcccccgact gcgtggtggt
     1081 gcttgatgat tcgctccttg acaccataga tgtggtagca gggttaaaac cgcagggcct
     1141 ggcaattatt aactcaaagg aaaaacccga agatattgac cttggcaaga tcatcaacac
     1201 ggcgactgtc aatgcaacat ccatagcgct tgagatcctg aaggccccga ttacaaatac
     1261 tgcaattctg ggggcatttg caaaagccac cggaattatc aagatagaat cccttgaaga
     1321 atccataaag agaaaatttg gagaaaagct gggggcaaaa gtgggtgaga gaaatgcgct
     1381 cgctgcacgg actgcctatg agaggacgat tacaggaaaa agcagggggg taaaaaagct
     1441 tgaagcaagg aaacaatggc tgccaacgca ccaggaaatt cccattggcg gatctcttat
     1501 tgaaggtaaa acggatgcag gtcttatcgg gcccgggagt tttatcgaga ataaggtttc
     1561 cggatgggcg acataccgcc ctttgaggga taaggcaaaa tgcacaatgt gtcttttatg
     1621 ctggttctat tgccctgaag gcaccatcgt tcggatttca gataggggag accttatgac
     1681 aaattatgat tattgcaagg ggtgcggcgt atgcgccaat gaatgcccgg tggatgccat
     1741 aaaaatggtg agggcgcgag tatgaagaaa gtaataacag gcgaccatgc tgttgcgatg
     1801 ggtgcaaaac tttgccgcgt tgaggtcata cccgctttcc caataacgcc gcaaacgcta
     1861 ataatagagc atattgcgga ttttgtaaat gacggtgaac ttgatgctaa gttccttacc
     1921 atggaatccg aacacagcgt aatgagtgca gcggctggcg catctgctgc cggcgcgagg
     1981 gttttcactg caacatcttc ccagggactt gcattcatgc atgagatgct ctatgccaca
     2041 gcgccattac gccttccgat tgtgatggca aatgccagcc gtacgcttgg gggacctccc
     2101 ggtatctggt gtgaatataa tgattcaatg ggtgcgcggg attcaggatg gctgcagctt
     2161 tacgtggaag ataatcagga ggcgctggat atggtgatcc agtctttcag gatcgcagaa
     2221 gacaaacggg tccttcttcc cgtgatgccc tgtcttgatg gttttgtgct tacgcatact
     2281 gttgaaccgg ttgatgtccc ggagcaggaa gaagttgatt catttctttt gccttatgaa
     2341 cctgatatca ttcttgaccc ggccagaccc gcaatgatcg ggactttcat gccttcagaa
     2401 tatatcatgg aattgcgaag gcagaccaca ggagctttcg aatctgcaaa ggaagttatc
     2461 cagcaagtga ataatgaatt tgcacaaaaa ttcggaaggg attatggggg tcttattgac
     2521 acatacatga tggaagatgc aagtatcgca ttgatcacaa tgggaacagt tacaagtaca
     2581 tcacgtgaag tggttgatga attaaggcaa aaaggagaaa aggttggtat tatcaagctt
     2641 cgttttttca ggccatttcc atcctatgaa ttgaaaaaag cggtatcgca tataagcgcc
     2701 ctgggggttt tcgaccgctc aatttcatat catggaggag ggccggtcta taatgaagtt
     2761 ctctcggctc tttatgggat gacaatacct gttatcaacc atctcgcagg tatcggagga
     2821 cgtgatgtga ccagggagca gataatgaaa atgttcgagt tgacacaaaa ggctggaaaa
     2881 ggggaaaaag tgaatgatat ctactggcat aatacgcggg gtgaaacagt atgatcacca
     2941 tcaaggaact ttccgatgtt aatcttttca ccgcaggaca tacggcttgc cccggatgcg
     3001 gtgtcgccat gtccatacgc aatgccatac gtgttcttgg aaaagatacc gtagtatatg
     3061 ttcccgcaag ctgtgcggtg gtatttggca cgacattccc ctatggcgcc tggaaagtgc
     3121 catttttcca taccgctttt gaaaatacag gcgcctgcct gtcaggaatg caggcggctt
     3181 ttgaaaagaa ggggaaaaaa gtaacggtcg tgggatttgc aggggacggg ggtacttatg
     3241 atatcggttt gcagagtatg tcgggtgcgg cagagcgtaa tgatgatgtc ctctatattt
     3301 gccttgataa tgaggcatat atgaatacag ggatacagcg aagcggcggg acaccttttg
     3361 gagcgtggac tacgactacg cccgttggta aggtcgtcca gggaaacagg cggtttaaga
     3421 aaaatctcgg cgatattgtt gcagcacatg aaattcccta ttatgccacg ctttccatcg
     3481 gacagcctct tgatttcata agaaaggtgg agaaggcaaa aaatatccgg ggtttccgtt
     3541 tccttcatat gttcactcca tgcataccgg gatggaagat ggacccggca aagacagtcg
     3601 agatcacaaa aagggctgtt gaaagtggta tgtggacgct ttacgagata gagaatggcg
     3661 agaaaaggat cactcacaag cccggaaaaa tgaaatccgt aaaagattac ctgatgatgc
     3721 agggccggtt caggcacatg agcgaagagg acataaagaa gctgcagatg tgggtatgca
     3781 agaaatggaa ccttcattat aaggagaaag gggcgccgga agtatgtgag attcttccac
     3841 cagaagaaca tcatgcagtc actgaagaac acagggagat gcacggacca tgaatataag
     3901 aatcaaacca agactgtaca
//
LOCUS       DATGBN010000151         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_360945, whole genome shotgun sequence.
ACCESSION   DATGBN010000151 DATGBN010000000
VERSION     DATGBN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360945"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VKL21_03440"
     CDS             <1..378
                     /locus_tag="VKL21_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44856.1"
                     /translation="SDPIDAYNVYLKNHHAVRIAKKDNKIPEFRIKIIKNEIERFTLK
                     YRKKVILGDRNLFISPLEMQIPLNLSHGSTKDIDEANFLYKLSKEKLNMTLMERFLSE
                     LNIPKESFEMFLNSKQPYLLSRL"
     gene            complement(341..1321)
                     /locus_tag="VKL21_03445"
     CDS             complement(341..1321)
                     /locus_tag="VKL21_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMB44857.1"
                     /translation="MDLFSHALFPYLLGNYFQKRKEEITAFVIGGIAPDVDIFLLWIQ
                     YLYPTFFLITHRGITHSLFFGFFTGIIVLYFASRNRVKKIVQKHIDFEPVFSRRNIVY
                     AFAGIIVHLILDYSTTRGVPLFYPFDVTRYSAELFFYTDIYLTIASLIMVIFLLRKPF
                     RIRNITRFLIIFLLILTGMGALRFAEKNNTELYFHDGTIKSFPTMNPFEWYAQKEEGT
                     KIDIYQYNGLEGKSQYNNTFQRMNVISGGGDLENALGLADELPQLKMFKWRAHSVAVN
                     ASYGNGTWVLEYYDPVQRAEMRDIPGTFRIVGARFSSLTINVTGDRAVSN"
     gene            complement(1321..2463)
                     /locus_tag="VKL21_03450"
     CDS             complement(1321..2463)
                     /locus_tag="VKL21_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMB44858.1"
                     /translation="MEEMPENETLFSRNFVLTSLSTFTLFTSFYFLLITLPIYIEKIG
                     GSESEIGLILGVFTISAVLLRPFIGKEVDRRGRKIILVSGIVIFFISMLLYDYTKNVT
                     SLLLLRVLHGIGWGAATTAATTLIADIAPPKRRGEAMGVFGMASNVAMAIGPALSMIL
                     LQKYDFPVLFSISAGISLISLLLVLPISETMVVHPKTPLFSREALFPSALMFMISLTY
                     GSIVSFLSLFAQKQGIANPGIFFTVFAVTLILVRALAGKLSDIKGRKFVIIPGMVLIA
                     AGLGVLSTASALDSFLVAALLYGIGFGLVHPSLMALLVDMVSDEGRGAAMGTFTAAFD
                     LGIGAGSIFLGLVLQYFDFQVMYMLSGLIALSGAVLLILFNGRRQM"
     gene            2631..>2936
                     /locus_tag="VKL21_03455"
     CDS             2631..>2936
                     /locus_tag="VKL21_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase"
                     /protein_id="HMB44859.1"
                     /translation="MIIIKLGGSLIQSAKRIVKDLIEYTNSTGESILIVPGGSIFADT
                     VRKVNPSQEAAHLMAILAMEQYGYYLADGMDVKLTDRLENTNGTFILLPYNLLKNNDE
                     "
BASE COUNT          955 a          612 c          574 g          795 t
ORIGIN      
        1 agcgatccca tagacgcata caatgtttac ctgaaaaacc atcatgcagt gaggatagca
       61 aaaaaggata ataaaattcc ggagttcaga ataaaaatca tcaaaaatga aatagaaaga
      121 tttacactga aatatcgaaa aaaagtaata cttggtgaca gaaatttatt tatttcacct
      181 cttgagatgc agataccgtt gaatttatcc catggttcaa caaaagatat tgatgaagca
      241 aattttctat acaaattgtc aaaagaaaaa cttaatatga cactgatgga gaggtttttg
      301 agtgaattga atatcccgaa ggaatccttt gaaatgttcc tcaattcgaa acagccctat
      361 ctcctgtcac gtttatagta agtgaactga atctggcacc tactattctg aatgttcccg
      421 gtatatccct catttcggcc ctctggacag gatcgtaata ttctaagacc catgttccat
      481 ttccataaga tgcgttaaca gccactgaat gcgccctcca cttaaacatt ttcaactgcg
      541 ggagttcatc cgcaagacca agcgcatttt caagatcccc tcctcctgaa atcacattca
      601 tgcgctggaa ggtattatta tactgggatt tcccctccaa cccattatat tgatatatgt
      661 ctattttcgt tccttcttct ttctgggcat accattcaaa gggattcatt gtgggaaagg
      721 attttatggt cccatcatgg aaatatagct ctgtattgtt tttctcagca aaccgcaggg
      781 ctcccatccc ggtcaatatg agaaggaata taataagaaa tcgtgttata tttcgaatcc
      841 ggaaaggttt cctcaataaa aatattacca ttatcaggct ggctattgtt aggtaaatat
      901 cagtataaaa gaacaattcg gcggaatatc ttgttacatc aaacgggtaa aatagcggga
      961 ctcctcttgt tgttgaataa tcaagaatca ggtgtactat gattcctgca aaagcataca
     1021 caatgttcct tcgcgagaat acgggttcaa aatcaatatg cttttgcact atctttttaa
     1081 cccggttacg ggaagcaaag tataatacta tgatacctgt gaaaaatccg aaaaaaagcg
     1141 agtgggttat gcccctatgg gtgatcagga aaaaagtagg gtatagatat tgtatccaca
     1201 aaagaaaaat atcaacatct ggcgctattc ccccgataac aaaagctgtg atctcctctt
     1261 tccttttttg gaagtaattc cccagcaaat atggaaataa cgcatgagag aaaaggtcca
     1321 ttacatctgc ctcctgccgt taaataatat cagtaaaacc gcgcctgaaa gcgctatcaa
     1381 accgctgagc atatacatga cctgaaaatc gaagtattgc aggaccagac ccaggaaaat
     1441 ggaccctgct cctattccaa ggtcaaaagc tgctgtaaac gtacccattg cagcgcctcg
     1501 gccctcatca ctcaccatgt caacgagaag agccatgagc gagggatgca caagcccaaa
     1561 accgatacca taaagaaggg ctgctacgag aaaactatcc agcgcgcttg cagtcgataa
     1621 aactccaaga cccgcagcga taaggaccat gcctggtatg atcacaaatt ttctcccctt
     1681 tatatcggag agcttacctg ccagcgccct gacaagaatc agggtcactg caaatactgt
     1741 aaaaaaaatt ccagggtttg ctattccctg tttttgtgca aaaagtgaaa gaaacgatac
     1801 tattgaacca tatgtcaatg atatcatgaa catcagggct gaagggaata acgcttccct
     1861 gctgaaaaga ggagtcttcg gatgcacgac catggtttca gatatgggaa gtacaagcag
     1921 caatgatatg agtgaaatac ctgcgcttat tgaaaaaaga acgggaaaat catatttttg
     1981 aagcaatatc atgctcaaag cgggtcctat cgccattgcg acattggatg ccattccaaa
     2041 tacacccatt gcttcgcctc ttctttttgg cggcgcgata tctgctatga gcgttgtcgc
     2101 agctgtagtc gcagcgcccc agccgatccc gtggagcact cttaagagaa gcaaggatgt
     2161 aacatttttt gtataatcat atagaagcat tgatatgaaa aaaataacta tgcccgatac
     2221 gagtatgatt tttcgtcccc gcctgtcaac ctcttttccg ataaagggtc gaagcaatac
     2281 cgccgatatg gtaaaaacgc ccaggatcaa tcctatttct gattccgaac cacctatttt
     2341 ttcaatgtaa attggaagtg tgataaggag gaaataaaaa cttgtgaaga gggtaaatgt
     2401 ggataggctt gtcagaacaa agttccttga gaagagtgtt tcgttttctg gcatttcttc
     2461 catttcatat ccttttttga aatacatcta taaaaatcca ctcttttcaa attttcagaa
     2521 gggaatgatt aaaatcattt tggtcaaggg tgagaagtca agtttatata gatttctatc
     2581 ttaacattgg actattgaca agtttgcatc aatgaattca atttaatatt atgataataa
     2641 taaaacttgg tggaagcctg atccagagcg caaagagaat cgtaaaagac ctcatagagt
     2701 acacaaactc aaccggggaa agcatcctca ttgtaccggg cggctcaata ttcgcagata
     2761 cggtgcgcaa agtaaatcct tcacaagaag ctgcccactt gatggcgata ctcgcaatgg
     2821 aacagtatgg gtattatttg gccgacggaa tggatgtgaa actcactgac agactggaaa
     2881 ataccaatgg aacattcatt ttgcttccat acaaccttct caagaacaat gatgag
//
LOCUS       DATGBN010000152         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_516192, whole genome shotgun sequence.
ACCESSION   DATGBN010000152 DATGBN010000000
VERSION     DATGBN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516192"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..988)
                     /locus_tag="VKL21_03460"
     CDS             complement(50..988)
                     /locus_tag="VKL21_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44860.1"
                     /translation="MSKNPLVVLLILIAVALSGCTGESSSKGTPVEVQKFDLANPNLI
                     GQIVDVKFDRDDIRAGEIATPELLIANTGSEKITNETVEITAKVKTLDDFLANLYLKT
                     MSEEKKTRIIDPLNFQTEIQPGSLKSISAQFHTIKEMEGRSLAGTYEINITLSVNGQK
                     VEARVFSITLHPGEVREITPTPTPSPTPTPIVTSTPTPTITKTEIPIPTPTSEPVVVA
                     EPTGKIIPINIKDDKFSITNIQIDVGDKVRWINYNPDDNYDIIEMDQKLPTIVLRDSA
                     SYLFNTTGDYKFGAYYRNMAKDPSTLTISVRVNASK"
     gene            1122..1601
                     /locus_tag="VKL21_03465"
     CDS             1122..1601
                     /locus_tag="VKL21_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMB44861.1"
                     /translation="MRTLAILLVLILLIIIPANAQTTQWYSYENGMTAANSMEKPVII
                     DFYADWCGPCVAMENSTYPDSRVVSEMKDFIAIKVNTQVRIDIESKYNIAYYPTIVFL
                     DPDGTEISRHVGYLGPEDMVKTIRDSRGKLPRETPGFQALYPILAISLLILSKRITG"
     gene            complement(1591..>2735)
                     /gene="thiI"
                     /locus_tag="VKL21_03470"
     CDS             complement(1591..>2735)
                     /gene="thiI"
                     /locus_tag="VKL21_03470"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HMB44862.1"
                     /translation="VRYDELALKSKNVRTRYEHILVRNLRSMLKSEGCIFSNITREMG
                     RIFIHSDDPNALKSASKVFGVVSVSPAYTCEATLASAAISSADIACDILKEGQSFAIR
                     ARRAGNHDFTSRDIGVACGDAVYEKMNRKIRVDLDDPEVEIFVELRQDKGYVFTGSVK
                     GVGGLPLGTQGKMVALVSGGIDSPVAAWLMMKRGCDIIPLYLNNDPFSDETTRQRAMS
                     CIDVLQKWSPHKKITIYEAPHGENLLAFLSNCNNRLNCVLCRRMMYRIAGEVMKMEDA
                     HGIITGSSLGQVASQTSENMLAEMHGMEYPIYHPLIGLDKLEITNMARNIGTLGPSTK
                     PATCCMAVPEYPSTAAKPGDVIEAEKLINVYQLVEHMIKNIKKFTR"
BASE COUNT          714 a          596 c          546 g          879 t
ORIGIN      
        1 ccgatatgat attgtctttt gactttaaaa agattatgaa ttaatgcaat cacttgcttg
       61 cattaactct tacactgata gtcagggtgc tcgggtcttt agccatgttt ctataatatg
      121 caccgaattt gtaatccccg gttgtattga atagatatga agcactgtcc ctcaggacaa
      181 tagttggtaa cttctggtcc atctcgataa tatcatagtt gtcgtccgga ttataattta
      241 tccatcgtac tttatcgccc acatcgattt gaatattcgt aatagaaaat ttatcatcct
      301 tgatatttat tggaattatt tttcctgttg gttcagcaac aacaactggc tccgaagtgg
      361 gggttggaat gggaatttca gtctttgtga tcgttggtgt cggtgtggaa gttactattg
      421 gggtgggggt tggagaaggc gttggggtgg gagtaatttc acgcacttct cccggatgga
      481 gtgtaatcga aaaaaccctg gcttcgactt tttgtccatt aactgataag gttatattga
      541 tttcataggt tcctgcaagg cttcttcctt ccatctcttt gatagtatga aattgcgcac
      601 ttattgattt taatgacccg ggttggatct ctgtttgaaa gtttaacggg tcaataatcc
      661 tggttttctt ttcctcgctc attgtcttta aatagagatt cgcaagaaaa tcatcaagcg
      721 tcttgacctt tgctgtgatt tccacagttt catttgttat tttttcagag cctgtatttg
      781 caataagtaa ttcaggcgtt gctatttctc ctgcccttat gtcatcccta tcaaacttca
      841 catcaactat ttggccaata aggttgggat tggcgagatc gaatttctgc acttccactg
      901 gcgtcccttt tgaagaactt tcacctgtac aaccggataa cgccactgct atgaggatca
      961 ataatacgac aaggggattt ttgctcattt agtcaccact acttagtaca gcgtccctgt
     1021 ttaaaaagat ttctcatcat tatagtgaga acaggtatta tcaaataacc ctcagaaatt
     1081 tttacaaata attttatacc ctgaaagcta attctgggtt aatgcgcaca ctagcaattc
     1141 tattagtttt aatcctttta ataattattc ctgcaaatgc ccagaccaca caatggtatt
     1201 cctatgaaaa tggtatgacc gctgcaaatt caatggaaaa acccgtaatt attgattttt
     1261 atgccgattg gtgtggtcca tgcgtagcca tggaaaatag tacttatccc gattctcgtg
     1321 ttgtttctga aatgaaggat tttattgcta ttaaagtaaa tacgcaggta aggatcgata
     1381 tcgaaagcaa atataatatt gcatattatc caacaatcgt gttccttgat ccggatggta
     1441 ctgagatttc acgtcacgtg ggatatctcg gacctgagga tatggtgaaa acgatccggg
     1501 attcccgcgg aaaattgcca agggaaacgc cgggattcca ggcattatac ccaatattgg
     1561 caatatcatt gttgatattg agcaaaagaa ttaccgggta aattttttga tatttttaat
     1621 catatgctcc acaagctgat agacatttat aagtttctca gcttcgatga cgtccccggg
     1681 ttttgctgct gttgagggat attccggtac agccatacag catgtggctg gctttgttga
     1741 gggccccaac gtcccaatat ttcttgccat gttagttatt tcaagcttat caagcccgat
     1801 aagaggatga tagatcggat attccatccc atgcatctca gccagcatat tttctgaggt
     1861 ctgcgatgca acctggccca gggaagaacc tgtgattatt ccatgtgcat cttccatttt
     1921 catcacttcc cctgcgatcc tgtacatcat gcgcctgcat agaacgcaat ttagcctgtt
     1981 attgcaatta ctcaggaatg ccaggaggtt ttcgccatgt ggagcttcgt aaatggtgat
     2041 tttcttatgc ggcgaccatt tctggagcac atcaatgcag ctcatggctc tctggcgtgt
     2101 ggtttcatcg gaaaatggat cattattcaa atacaggggt attatatcgc aaccgcgctt
     2161 catcattaac catgcagcca ccggtgaatc tatacctcct gaaacaagag ccaccatctt
     2221 gccctgtgtc ccaagcggca gcccccccac acctttaacc gatcccgtga acacatatcc
     2281 tttatcctgc ctcaattcca cgaatatttc tacttcagga tcatccaggt caaccctgat
     2341 tttccggttc attttttcat ataccgcatc cccgcaggct acacctatat cccttgaggt
     2401 aaaatcatgg tttcccgctc ttcgcgccct tattgcaaat gattggcctt ccttcaggat
     2461 atcacatgca atatctgctg atgagatcgc tgcagacgca agtgtagctt cgcaggtata
     2521 agcgggactt acggatacaa ccccaaagac tttcgaagcg ctttttaagg cattggggtc
     2581 gtccgagtgt atgaaaatac gccccatttc acgggttata tttgaaaaaa tgcagccttc
     2641 tgatttgagc attgatctta aattcctgac cagtatatgc tcataccttg tccttacatt
     2701 cttactcttg agggcaagtt catcatatct gacta
//
LOCUS       DATGBN010000153         6403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_244265, whole genome shotgun sequence.
ACCESSION   DATGBN010000153 DATGBN010000000
VERSION     DATGBN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244265"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VKL21_03475"
     CDS             <1..676
                     /locus_tag="VKL21_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMB44863.1"
                     /translation="VARDITERKLAEDQIAQSLREKEVLLREIHHRVKNNMQIISSLL
                     SHQMENINDKKIIDIFTESQNRILSMSLVHEKLYQSRDLRNIDFKEYINDLGINLFQS
                     YDIHSGNIKLNIDVEDILLDIDLAIPTGLIINELITNSLKYAFPDGMKGEIKVLFRST
                     NENMLELVVSDNGIGLPEDLDFRKTRTLGLHLVTVLAENQLHGNIDMDRIKGTEFRIK
                     FRGIKS"
     gene            673..1509
                     /locus_tag="VKL21_03480"
     CDS             673..1509
                     /locus_tag="VKL21_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB44864.1"
                     /translation="MTPITQILVVEDEIIIAEDLQIKLIRMGYSVPDIVSSGEEAIKK
                     VKENNPDIILMDIVIHGKIDGIETVEKIHAFSDVPVIYLTAYADQYTLERAKITEPFG
                     YLLKPFKERELLITLEMALYKHNMEKKLKESEKRLRERERWLTAAIKSIGDGVIATDL
                     TGSIKIMNPLAEALTGWKKEDALCKPLLAIFNIKSQENNKNVEDPVAKVIREGSFYGL
                     SEQTILITKNKLEIPVDIIGSPITDDNNDIIGVVLVFYDIIERKVIETKMRTGSINSS
                     ET"
     gene            1600..2022
                     /gene="nikR"
                     /locus_tag="VKL21_03485"
     CDS             1600..2022
                     /gene="nikR"
                     /locus_tag="VKL21_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HMB44865.1"
                     /translation="MEQDLSRIGVSLPENLLEKFDEIITKRGYSSRSEGIRDAIRGYI
                     RYYDWMREVEGERIGILSLTYDHSQRGLLSSLIEVEHEFINIMRSSVHIHINHDECME
                     VLILQGDGKEVKVTAERIMSLKGVKNVKLTTIKPGQEI"
     gene            complement(2082..3356)
                     /locus_tag="VKL21_03490"
     CDS             complement(2082..3356)
                     /locus_tag="VKL21_03490"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HMB44866.1"
                     /translation="MVKTSKISGFYKQSPEERLKIVSEFSGLTEEEASCIGATGALSL
                     DAADRMVENLIGVMEVPMGVAVNFLINGRDYLIPMAIEEPSVIAAASNSAKMAREGGG
                     FATSSTGPVMMGQIQAVGIKDPNGARLNILEKKDEIIKRANEVDPILVKFGGGAKDVE
                     VRVIDTPQGKMVITHLVVDCRDAMGANAVNTMAETVAPYIEELTGGRVYLRIISNFAD
                     KRLMRAKAVFPKEVIGGEEVVDGILAAYHFATGDPYRAATHNKGIMNGVSAAVLATGN
                     DTRAIEAGAHVYASRSGVYRPLSCYEKNANGDLVATLEMPMAVGLVGGATKTNPITRA
                     AVKILGVKSATEMGEVFAALGLAQNFAALRALATEGIQRGHMGLHSRNVAIQAGATGD
                     LVDKIALIMVREKKVRADRAKELLDELMAGTK"
     gene            3477..3629
                     /locus_tag="VKL21_03495"
     CDS             3477..3629
                     /locus_tag="VKL21_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HMB44867.1"
                     /translation="MTQRTKAKKKRLAKHQNQNQRLPAWVIVKTKRNVMTHPKRRNWR
                     RSTLKV"
     gene            3640..3903
                     /locus_tag="VKL21_03500"
     CDS             3640..3903
                     /locus_tag="VKL21_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HMB44868.1"
                     /translation="MADIERIYTIPLSDARKVPRWKRAECAVRKVRAYLGKHLKTDIT
                     DVKIDKTINEKLWERGSMKPPLKLRVRAVKFEAGGVQAELAQE"
     gene            3925..4584
                     /locus_tag="VKL21_03505"
     CDS             3925..4584
                     /locus_tag="VKL21_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HMB44869.1"
                     /translation="MKRKLNISGSSVLGVFASCTDEFVFVPYEASGETKSELEESLGV
                     AACRTSVSGSSIVGSLMRGNNTGMMVAGFVLEKELRTIRKHIKAARLTQELNAAGNLI
                     LANDTAALVHPDLSEKSIKIIQKTLGVEVKRGTIGGLKTVGMAGVATNRGVLVHPKSS
                     PSEISIIEELFKLPVDIGTVNFGSPLVGSGLLANNKGYVVGEETTSPEISRIEDILGY
                     L"
     gene            complement(4617..4769)
                     /locus_tag="VKL21_03510"
     CDS             complement(4617..4769)
                     /locus_tag="VKL21_03510"
                     /inference="COORDINATES: protein motif:HMM:NF036315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5371 family protein"
                     /protein_id="HMB44870.1"
                     /translation="MKFIHVQSVLPQKDVIALKEKSGESSVKEAISRAVYHYLKCELA
                     NKDEKS"
     gene            complement(4981..5289)
                     /locus_tag="VKL21_03515"
     CDS             complement(4981..5289)
                     /locus_tag="VKL21_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HMB44871.1"
                     /translation="MVETKKSFIKEFNDFLTKYGVIGLAVAFVMGLAITKLITALVAD
                     LIMPIIGALIPGGNWRLATLDVGSIKFMVGDFAGALIDFIIIAIVIFMIVKNIVKEIK
                     "
     gene            complement(5349..>6403)
                     /locus_tag="VKL21_03520"
     CDS             complement(5349..>6403)
                     /locus_tag="VKL21_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMB44872.1"
                     /translation="YSWIIIASAYAPDLDIIADTLLNKLGISLLMDGSPIKHGDFHNI
                     IMLLLFAIFVSLILQAKGYRFAEAFTFACIGFGAHMFEDGLVYNPGYPFLRPLSEHIF
                     GIGIFDYKPDLYLFADTRVLIFGLIAVMLSTAIRIAYERREWINKEIKIILIIFAVLI
                     LVVPVLSALDISVVDEMTSSKGGVLLENWQFGKYTSWDSTIFHSGNHSVKIMVPGNES
                     KISGIFQSTVIPAQPDTTYLFSSWGRTRDAYGTNSPAVRIVELDRNKKFIKQTNLIYE
                     KGTNNWIKKNISIRSSARTRWVYVYSNIWKGYGTFWFDDVGLYEKGTDTNLIPDPGIE
                     NGHMIELVRFPLFSVK"
BASE COUNT         2029 a         1323 c         1280 g         1771 t
ORIGIN      
        1 tgtagcgcgc gatataactg agcgcaagct ggctgaggat caaattgcac aatctctccg
       61 ggaaaaagaa gtgcttctaa gagaaattca tcatcgcgtt aaaaataata tgcagattat
      121 ttccagtctg ttatctcatc agatggaaaa tatcaatgat aaaaagatca tagatatttt
      181 cacagagagc cagaatagaa tattatccat gtctcttgtc catgaaaaac tctatcaatc
      241 aagagacttg aggaatattg atttcaaaga gtacataaat gatctcggaa tcaatctatt
      301 tcaatcctat gatattcatt caggaaacat aaaattaaat atcgatgtgg aagatatatt
      361 attggatatc gatcttgcaa ttccaaccgg attgataata aatgaattga tcaccaattc
      421 attaaaatat gcatttccgg acggcatgaa aggggaaatc aaggtcttat ttagatcaac
      481 gaatgaaaat atgcttgaac ttgtggtcag tgataatggt attggtcttc ctgaagatct
      541 tgatttcaga aaaaccagaa cgctgggttt acatcttgtc acagttcttg cagaaaatca
      601 actacatggt aacatcgata tggatcgcat taaaggaaca gaattccgga ttaagttcag
      661 ggggataaaa tcatgacgcc aataacacag atactggttg ttgaagatga aatcattatt
      721 gcggaagatc tccagataaa attgataaga atgggatatt ctgttccgga tatagtatct
      781 tcaggagaag aggccataaa aaaagtaaaa gaaaataatc cggatattat tctgatggat
      841 attgtcatac atggaaaaat agatgggatt gaaaccgtag aaaaaatcca tgcattttca
      901 gatgttccgg ttatatattt aacggcttat gctgatcaat ataccctgga acgagcaaaa
      961 ataaccgagc catttggata tctcctcaag ccattcaaag aaagagaatt attgatcaca
     1021 ctggagatgg ccctatacaa acataatatg gagaaaaaat taaaagagag cgagaagaga
     1081 ctcagagaga gagaaagatg gctcactgcc gcaattaaaa gcataggtga cggagtaata
     1141 gccacagact taactggatc aataaaaatc atgaatcctc ttgcagaagc cttaacgggg
     1201 tggaaaaaag aagatgcttt atgcaaacct ctcctggcaa tattcaatat taaaagtcag
     1261 gaaaacaaca aaaacgtaga agaccctgtt gcgaaggtta tccgggaagg aagtttctat
     1321 ggcctgtcag aacagactat attgataaca aagaataaac tcgaaatacc tgttgatata
     1381 atcggttctc ctattacgga tgataataac gatattatag gggtggtact ggttttctat
     1441 gatataattg aacgtaaagt gattgaaaca aaaatgagaa caggatcgat aaattcatca
     1501 gagacgtaat atttttcatt ttaaaaaaaa atcatttgtt tccaaaaaat gcaaacgatt
     1561 atactgtacg gcttcaattg ttgataaagg agacattata tggagcaaga cctgtcaaga
     1621 attggagttt ccctgcctga gaacctgctt gagaaattcg atgagatcat aacaaaaagg
     1681 ggctattcat ctcgttctga ggggatacgc gacgctataa gaggttatat ccgctattac
     1741 gactggatga gagaggtaga aggagagaga ataggcatat tatccctgac ctatgatcat
     1801 tcccagcgtg ggcttttatc ttcgctgatt gaagtggagc acgagttcat aaatattatg
     1861 aggtcatcgg tacacatcca cataaaccat gatgaatgca tggaagttct catattacag
     1921 ggggatggga aagaagtaaa agtaactgcc gaacgcataa tgtcattgaa aggcgtgaag
     1981 aatgtgaaac tgaccaccat aaaacccggg caggaaattt gacattctcc tgaaagccac
     2041 ttattttcat gggaaattac aagcaaattt attaattaca ttcactttgt tccagccatc
     2101 aactcatcca gcagctcctt tgccctgtca gccctcacct tcttttccct gaccattatc
     2161 agggctatct tatcaaccag atcccctgta gctcctgcct ggatagcaac gttccttgaa
     2221 tgaagcccca tatggcctct ctggatacct tctgtggcaa gcgcccgcag agccgcaaaa
     2281 ttctgggcca ggccaagggc tgcaaatacc tctcccatct ctgtagctga ctttacaccc
     2341 aggatcttta cggcagcgcg tgttatcggg tttgttttcg tagctccgcc cacaagaccc
     2401 acagccattg gcatctctaa tgttgcaaca aggtcgccgt ttgcattctt ctcataacac
     2461 gataagggac ggtaaacccc gcttctggaa gcatatacat gcgctcccgc ttcaatcgcc
     2521 ctggtgtcgt tgcccgtggc aagcacagct gctgaaacgc cgttcatgat acccttgtta
     2581 tgtgttgccg ccctgtatgg gtcacctgtt gcaaaatgat atgctgcaag aataccatcc
     2641 acaacttctt cccctccaat aacttctttt ggaaaaaccg cttttgctct catcaggcgt
     2701 ttatctgcaa aatttgatat tattcgcaaa tatacgcgcc ctccggtcaa ctcttcaata
     2761 taaggcgcca cagtttcagc cattgtattc acggcatttg cgcccatggc atccctgcaa
     2821 tccaccacaa gatgcgtgat gaccattttc ccctggggcg tatcaattac cctgacctcc
     2881 acatcctttg ccccgccgcc gaatttaaca agtatcgggt caacctcgtt ggcccttttt
     2941 attatctcat cttttttctc aaggatattc aatctcgctc cgttggggtc ttttatacca
     3001 acggcctgta tctgccccat cataacagga cccgtgctgc tcgtggcaaa gcccccgcct
     3061 tcccgcgcca tctttgcaga gttgctggcc gctgctataa cagagggttc ttcgatggcc
     3121 atgggaatga gataatccct gccgtttatc aggaaattca cagccacacc cattggtact
     3181 tccataaccc caatgagatt ttccaccatt ctgtccgcag catcaagtga taaggcgcct
     3241 gttgcgccga tgcatgatgc ttcttcttct gtgagacctg agaattcgga tactattttg
     3301 agtctttctt ccggactttg tttataaaaa cccgatatct ttgatgtttt taccataaat
     3361 atatcacctg caagaggatt ttagatggca ggcgggtata aataagtacc caaaagcttt
     3421 atcaatgtat caagacgtag agggtattcc aatcctagat tattcggtga attcttatga
     3481 ctcaaaggac aaaagcaaag aaaaaaagac tggcaaaaca ccagaaccag aaccagcgcc
     3541 tccctgcatg ggttattgta aagactaaga gaaatgttat gacccaccca aagagacgca
     3601 actggagaag aagcacactc aaggtttaaa gtgattatta tggcagatat tgaacgaatt
     3661 tatactattc ccctttctga tgcaaggaaa gttccacgct ggaagagagc ggaatgcgca
     3721 gtaagaaaag taagagcata cctcgggaaa cacctgaaga ccgacattac cgatgtgaag
     3781 atcgataaga caatcaatga gaaactctgg gagagaggct caatgaaacc gccattgaaa
     3841 ttaagagtaa gggcggtcaa gttcgaggct ggcggcgttc aagcggaact cgcccaggaa
     3901 taatcttttt ttaatggggc ataattgaaa cgaaagttaa atatttccgg aagctccgtg
     3961 cttggcgtgt tcgcatcctg tacggacgaa tttgtttttg taccttatga ggcatccggg
     4021 gaaacaaaaa gtgaacttga agaatcactt ggagtagccg cctgtcgcac ttcagtaagc
     4081 ggaagttcca ttgtgggttc attgatgcgg ggaaataata ccgggatgat ggtagcaggc
     4141 tttgttctgg aaaaagaact caggactata aggaaacata tcaaggcggc aaggttgaca
     4201 caagagctaa atgcggcagg gaatctcatt ttggccaatg ataccgcggc gcttgttcat
     4261 cctgacctct ctgaaaagtc catcaagata attcaaaaaa ctcttggggt agaagtgaaa
     4321 cgaggaacaa tcgggggctt aaagaccgtg ggaatggcgg gagtagccac gaacaggggt
     4381 gtacttgtgc atcctaaatc aagcccttca gagatttcga taattgaaga actatttaaa
     4441 ttaccagtag acatcggtac ggttaatttt gggtctccac ttgtaggttc aggacttctg
     4501 gccaataaca aaggttatgt ggttggtgag gagactacca gtcctgagat aagccggatc
     4561 gaagatatat tgggatactt gtaggcagag tcttcgagga atgtgtttca ggcctattat
     4621 gatttttcat ctttattggc caactcacac ttcaaataat ggtatactgc cctggagatt
     4681 gcttctttga ctgaagactc accggacttt tctttcaaag caattacatc tttctggggg
     4741 agtacagatt gaacatgtat gaatttcata ttataattcc caagattaaa taaatctcta
     4801 cccataagta taattcatgg attaatatac tattaccctt taaacatata aatatatcgc
     4861 tatttaataa taattatgat gctaatagta atcatataat ataattctta aagtttatcg
     4921 gtcttaaacc tgaaattgac actctccacg cttaaagttg aattcctgca aaaaaacctg
     4981 ttattttatt tctttgacga tattttttac gatcataaat attacaattg caattattat
     5041 gaaatcaata agggcacctg caaaatcgcc caccatgaac tttatacttc caacatccag
     5101 ggtcgcaagc ctccagttgc ctccagggat caatgcacct attataggca ttataagatc
     5161 agctacaagc gctgtaataa gctttgttat tgcaagaccc attacgaacg caaccgctaa
     5221 cccgataact ccatactttg tcaagaagtc attgaactcc ttaataaaag atttcttagt
     5281 ttccaccatt caattacctc ctaataatta aatatatatt gtagtatata aggatgtcat
     5341 aaccaaattt atttcaccga aaaaagtgga aaacggacta attcgatcat atggccattt
     5401 tcaattccgg gatcggggat aagatttgta tctgttccct tttcatataa tccaacgtca
     5461 tcaaaccaga aagtcccata ccctttccag atatttgaat aaacataaac ccatcttgtt
     5521 ctggcgcttg atcgtatcga aatattcttt tttatccaat tatttgtccc tttttcgtaa
     5581 ataagatttg tttgctttat gaatttctta ttacgatcaa gttctactat tcttacagcg
     5641 ggggaattgg ttccatacgc atccctggtc cttccccagg atgagaacaa ataggtcgta
     5701 tccggctggg cgggaattac cgtactctga aaaattcctg atatcttgct ctcattccct
     5761 ggaaccatta tcttaactga atggttgcca ctatgaaaaa ttgttgaatc ccatgaggtg
     5821 tattttccaa actgccaatt ttcaagtaaa acccctcctt tgcttgaagt catttcatcg
     5881 actacagaaa tatcaagagc gctaagaaca ggtaccacca ggatcaatac tgcaaaaata
     5941 attaaaatta ttttgatttc tttgtttatc cattcccgtc tctcataagc aatccttatg
     6001 gcagtcgata acatcactgc gatcaggcca aagatcagca cacgtgtatc agcaaaaaga
     6061 tacaaatcag gcttataatc aaatatgcct atcccaaaaa tatgttcaga tagcggccgc
     6121 aagaacggat acccgggatt gtaaactaaa ccgtcttcga acatatgagc cccgaatcct
     6181 atgcatgcga acgtgaaagc ttcagcaaac ctgtaaccct tagcttgaag aatgagagaa
     6241 acgaaaatgg caaaaagtaa cagcattatg atattatgaa aatcgccgtg cttgatcggg
     6301 gatccatcca taagcaagga aatacccagt ttatttaaaa gggtatcggc tataatatcg
     6361 agatcgggag cgtaagcgct tgcaatgatg atccaggagt agt
//
LOCUS       DATGBN010000154         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_477069, whole genome shotgun sequence.
ACCESSION   DATGBN010000154 DATGBN010000000
VERSION     DATGBN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477069"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VKL21_03525"
     CDS             complement(<1..353)
                     /locus_tag="VKL21_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HMB44873.1"
                     /translation="MVVGTGSHVGKSILVTALCRILSKRYSVAPFKAQNMSLNSWVTR
                     EGDEIGIAQAIQAKAAGVEPTADMNPVLLKPKGDHRSQVIILGKPVADKEAGDYYDSI
                     DDVFSTVAGAYDRLC"
     gene            complement(382..1008)
                     /locus_tag="VKL21_03530"
     CDS             complement(382..1008)
                     /locus_tag="VKL21_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HMB44874.1"
                     /translation="MKTIDELIQKAVELQAGGLLTGQIADELNVSRETATWLLVHSKK
                     GDATAAPKDIYIDWSNIGRSANRQRLIASSLTDMIIECLSKTQNDIHVIVGIALSGIP
                     LANMVADELGVEFATYHPNKQKWEPESKDIGGTISQNFAKVAGKNCVLIDDVVTSGTT
                     ITEAVAVLENLGARPVAIAVLIDKKGIDEISGVPLNALLRVTRVDKTE"
     gene            1123..1548
                     /locus_tag="VKL21_03535"
     CDS             1123..1548
                     /locus_tag="VKL21_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44875.1"
                     /translation="MAIDILQSIVGGITASLVWFMAGGVLYMNPFVAKIYEDARKSPG
                     LKKWTNVPKYLSFQFYGILAQCLLWSFVFAFVKPVLPEGIILKGGTFGLILVAVKIFP
                     RFVDMWTQSTYPDKLLVVEFVNGTIGSFIIGVVFAYLIR"
BASE COUNT          529 a          395 c          384 g          610 t
ORIGIN      
        1 tcgcaaaggc gatcatatgc accggcaacg gttgaaaaaa catcatcgat cgaatcataa
       61 taatcccctg cttccttatc agctacaggt tttccaagga tgatcacctg tgacctgtga
      121 tccccttttg gttttagcag caccgggttc atatccgctg tcggttcaac tcctgccgct
      181 ttagcctgta tcgcctgagc tattccaatt tcgtctccct cacgtgttac ccatgagttc
      241 aggctcatat tttgtgcctt gaacggagca acagaatatc tttttgagag gatcctgcat
      301 agtgctgtca caagtatgct ttttcccaca tgtgagccag tgcccacgac cataaggctt
      361 ttagtctttt ttttttcatc cctattcggt tttgtcaact cttgttaccc gcagtaatgc
      421 attaagagga acacctgaaa tctcatcaat acctttttta tctataagta ctgctatagc
      481 taccggtctt gccccaaggt tctcaagaac cgccaccgct tcggtaatag ttgtacctga
      541 tgttacgaca tcatctataa gtacgcaatt ctttcctgcg acttttgcga agttctggct
      601 tattgtccct ccgatatctt ttgattccgg ctcccatttc tgtttattcg gatgatatgt
      661 ggcaaattcc actccgagtt catcagctac catatttgca agagggattc ccgaaagcgc
      721 aatccctacg ataacgtgga tatcgttttg cgtcttgcta aggcattcga tgatcatgtc
      781 agtcaatgag ctcgctatca gcctctgcct gtttgcgctt ctcccaatat tgctccagtc
      841 tatgtaaatg tccttggggg cagcggtagc atcgcccttt ttcgaatgga caagaagcca
      901 tgttgctgtt tcacgggaaa cattcaattc atccgctatc tggcctgtca aaagtccgcc
      961 agcctggagt tctacagctt tttgaattaa ttcatcgatt gttttcataa ctatttcacc
     1021 ggaaatgata ttaattcaat ttctttgtat ttaatagatt gtctggaaat ccccaacccc
     1081 acgttataaa tattaattaa ttaatactta agtgagatta atatggcaat agatatactt
     1141 cagtcaatag taggtggtat tacagcatcg ttggtgtggt tcatggcagg tggtgttttg
     1201 tatatgaatc cctttgtagc gaaaatatac gaggatgccc ggaaatcacc gggattaaag
     1261 aaatggacaa atgttccgaa atatcttagt ttccagtttt atgggatact ggctcaatgt
     1321 ctgttatggt cttttgtttt tgcctttgtt aaacccgttc ttcctgaagg aataatacta
     1381 aaaggaggaa cgttcggact tatccttgtt gctgtaaaaa tatttccaag attcgtggat
     1441 atgtggactc aatcaactta tccggacaaa ttacttgtcg ttgagtttgt caacgggaca
     1501 atcgggagtt ttataattgg tgtggttttt gcatatctta ttaggtagca gacacagaaa
     1561 gacttaacat atatcaaata ataaatatgc tgggaggtgt taagaatggt taccaaaaaa
     1621 ccaaaagttg atgaagcaga aaatgagaga ttaaaaaagt tcaatgaaca attaagaaac
     1681 cgtaaagcac cacagccaaa agctaaatag atatctgctc agggttcaag gagtaggaaa
     1741 cttcagttac tcaggaaaga tagaagtcta ctccttaatt tttccacaag ccatattgat
     1801 ttggataggg caggcattgg ataggtgtgt tgtgggagtg ttataaaaag attgcctgcc
     1861 catactcact ataatcataa tgatatttat aggctttcgg tcgctaaaat gaagataa
//
LOCUS       DATGBN010000155         8013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_322021, whole genome shotgun sequence.
ACCESSION   DATGBN010000155 DATGBN010000000
VERSION     DATGBN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322021"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VKL21_03540"
     CDS             complement(<1..899)
                     /locus_tag="VKL21_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HMB44876.1"
                     /translation="MNINRIIVCVLAVLTLMMSISVPNAAALVIEKSLGEMTTEADII
                     VTGKVVNKESYWENGKIFTDVTISVGQKIKGRPENEIIVQVQGGVVGDIYAQVSDVPL
                     FEENEEVLLFLKGNQVVGWNQGKYTIKAEKIIENDELVTEHEKSDAGYAIPSKAPHIT
                     KVTPNSGPAKALFLGSTTAATNSTQITITGSNFGMLEGGVQFWRVGTISYDATIVSWT
                     DTKIVAKVPGGISSHFKPDGTGNIRIIKNDGTPSDNYGRFHVTYSYNGARWPGSSATY
                     MINPNTPDTAGELAAVKAAANMW"
     gene            complement(941..1606)
                     /locus_tag="VKL21_03545"
     CDS             complement(941..1606)
                     /locus_tag="VKL21_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44877.1"
                     /translation="MFYYESGSRYAPEEKVGRSNIFWIFLIISIMALVIVTGCIDREK
                     KNTDIGPSTPEKTPITVISSSSEGIMEKFNLQKLVSMSDSIVIGKVTEVFQSEWNTPD
                     GKKPVNNPASNIIYTDVNIKVLEYITNPLDSTAITVRMLGGIVGQDSLIIEDQPSYST
                     GETVLLFLKNDDDPRTKDIGDKHFVTAGLAQGKISILQNNQTVIGDEKMSLDDLRARI
                     TGK"
     gene            1824..2174
                     /locus_tag="VKL21_03550"
     CDS             1824..2174
                     /locus_tag="VKL21_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44878.1"
                     /translation="MAIKTKGISTTSVSSWWEERRKVLASNTKPVRVKTKSIKGKPKM
                     VKAKAKPVRVKPKTVKAKSKQVRAKPKTVKAKSKQVRTKPVTKARTASKKKKIVKTVS
                     KTRKVTLTKKAKKK"
     gene            2377..3117
                     /locus_tag="VKL21_03555"
     CDS             2377..3117
                     /locus_tag="VKL21_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44879.1"
                     /translation="MISVAYGITYIFQTGTIVFPFGPYYFLFIFSIGFVIFTVYFERE
                     KKAVYPRELIGGAMASSCLTFLIIACVAGMKYIWEKGLSGIGIETLAYAFSISMILSL
                     ILYFVLGKIEVKFVPGDFTRVLSIVLGMAILIMITVVYGINYIIKIDPFPLKPYVLLF
                     IFAIVFVLASVFYEKRGALYPWFLLGGAVASACFVFIIAAIIGGLRYVLDKGFTGLGT
                     DTVFYSLSICIILSMIIYIQYNIIKEKL"
     gene            3162..4898
                     /gene="mutL"
                     /locus_tag="VKL21_03560"
     CDS             3162..4898
                     /gene="mutL"
                     /locus_tag="VKL21_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMB44880.1"
                     /translation="MGNIRILDEASINKIAAGEVIERPASVVKELIENSIDAGADTIR
                     IEVTKSGKDSIRVADNGCGMSRDDAALSYVKHATSKIRKIDDIETVRTMGFRGEALSS
                     ISAIARVEITTKTKNELSGTKIIIHGGKLVSILETGAPDGTAILVKDLFYNTPVRKKY
                     LKSDNSELTRIIDIVSRIAIGHNNISFSLFNNGKELLRSPASELPGTIIHIYGQEVAR
                     AMLPVDLESPIVRINGFISRPSLMRSSLDFQSFYVNDRNINSRAIGFALREGYGTLMP
                     RGRFPIAVLKINVDTHEVDVNVHPTKNQVRLSHERDICDMVKQAVKNALSKNDLIPDI
                     KIPLQQPLLYETPAADPLQVKETINYFKPSAKDTQRRLRRSDRAGMEIVKEQNNEMPD
                     MKVIGQVDSIYILAGTKNGLMIIDQHAAHERVFFELVRESKRDDSQELIVPVNIELDS
                     REKVLIKDTIPYLEEFGFRISEFGPDSFAVTAVPIVLGKLEDAQIVHDIISDILSEGK
                     IKEETGIFERVTKSIACRSALKAGADCSMSQMENLIMQLFRTDNPYTCPHGRPTMVSF
                     NRQELDKLFRRS"
     gene            5068..6153
                     /locus_tag="VKL21_03565"
     CDS             5068..6153
                     /locus_tag="VKL21_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44881.1"
                     /translation="MVKKHNNSQEFEVNGDVHKKWSDTYGSATKIWDESHSRLMKPWI
                     ELMGEMSERTKEISINAAPKNYKEFYNEWMKTYENSFGKFYPVSIPGPKETLENFIKI
                     SGESNKLYRLWIEESEENAKRTSELLINGADPAKFKECYEMWMKSYDKMSNDFIEQPA
                     VKYQKEVFGNYTGIPDFYSETLVKMSTMWRDSFNKLYNPWIETMQKLSMNVDKISRGN
                     AGPETYKEFFDLWADTYKENFEKMFDPQSSITSREALDNFIESSEISLNLYKSWILAL
                     ENMHEKFKDHSKITTDPGAYKEFFNLWVKMHEKALEDVFDRMPMVSPVKEMMEPIRDA
                     CKIYAKASIKMSKMWLDSYTRAACPEV"
     gene            6189..6929
                     /gene="fabG"
                     /locus_tag="VKL21_03570"
     CDS             6189..6929
                     /gene="fabG"
                     /locus_tag="VKL21_03570"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF004198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMB44882.1"
                     /translation="MRVENKVVIITGSGSGIGRETALLFGKEGAKVVVADVNEKAGEE
                     TAEEIRQNGSDAFFAKLDVSDREQSKQVVKDTLERFGKIDVLINNAGIIQDALISKMS
                     EDQWDRVIDIDLKGPFNCIQAVVEAMTNHGTGEIINVSSIVALYGNIGQSNYSSAKAG
                     LIGLTKTLAKELGRKGIRVNAVAPGFIHTPMTATVPEKILEMMKEKTPIKRLGQPDDV
                     AYALLYLASDEANFVNGAVLSVDGGLLL"
     gene            6943..7551
                     /locus_tag="VKL21_03575"
     CDS             6943..7551
                     /locus_tag="VKL21_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMB44883.1"
                     /translation="MKQKETKISNLKKALVKALKIDEKKLAGRIKAIGFECRKCAQCC
                     SAQYGDNTVIVFPSEIIRICDKTGLSRDEFVIPTPSQDRDSQGNIHTFEWVLKKNCDC
                     IFLKNDLCEIYECRPYICSTYPFYLLNGMLEISECMGIGGNISTQECLMLAALLKERY
                     INEIRESIALLERFDGFNPGKSGNLCVHDSEGEHWMHYNYKH"
     gene            7569..>8013
                     /locus_tag="VKL21_03580"
     CDS             7569..>8013
                     /locus_tag="VKL21_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB44884.1"
                     /translation="MKLLALSDLHGDYSHIGSILKRAGEIDAVLIAGDLTDFGPDDKA
                     RELLEMFKVPVLAVPGNCDNPSLLKVLDKNENTINLHNSLHTIGELTFIGLGGSNPTP
                     FNTPFELSEKKIGEYVGTLLSGSKGRMILLSHAPPLNTMDKLPHGN"
BASE COUNT         2538 a         1543 c         1639 g         2293 t
ORIGIN      
        1 ttccacatat ttgcagcagc cttaaccgct gctaattcac cggcagtgtc aggcgtattt
       61 ggattgatca tatatgttgc actacttcca ggccatcttg cgccattata tgaataagta
      121 acatggaaac gtccataatt atcactgggg gtaccatcgt tttttatgat ccggatattt
      181 ccagttccat cgggcttgaa atgactacta attcctcccg gtactttagc gactatttta
      241 gtatccgtcc atgatacaat tgtggcgtca taacttatag ttcccacacg ccagaattgt
      301 accccccctt ccaacatacc aaagttcgaa cccgtaatag ttatttgagt actgtttgta
      361 gcagcagtag tacttcctaa aaataaggct tttgccgggc cagaatttgg agtaactttt
      421 gtaatatgcg gcgcctttga tggtatcgca tatccggcat ctgatttttc atgttctgtc
      481 actaattcat cgttttctat tatcttttca gccttaattg tatacttccc ctggttccat
      541 ccaacgacct gatttccttt taaaaaaagc aaaacttctt cattttcttc aaacaatggt
      601 acatctgaga cctgggcata tatatctcca acaactccac cctgtacttg tactattatt
      661 tcattttccg gtcttccttt tattttctgc cccactgaga ttgtaacatc tgtaaatatc
      721 tttccgtttt cccaataaga ttccttattt accacttttc ctgttacgat aatatctgcc
      781 tctgtcgtca tttcacccag gcttttttca attactaacg ctgctgcatt tggtactgat
      841 atggacatca ttagtgtcaa tacggctaat acacatacaa ttattcgatt aatattcata
      901 tatatttcct catattttat tcaaattatt gtcttttctt ttactttcct gtgattcttg
      961 ccctgaggtc atcaagtgac attttctcgt ctcctatgac ggtctgatta ttttgtaaaa
     1021 ttgatatttt tccctgtgca agccctgccg ttacaaaatg tttatcccca atatctttgg
     1081 ttctgggatc atcatcattt ttcaaaaaaa gaagcactgt ttcgcctgta ctgtaagaag
     1141 gttgatcctc tattattaga ctatcctgtc ctacgattcc ccccaacatg cgtactgtta
     1201 ttgctgttga atccagtgga ttcgttatat actccaatac ttttatatta acatcagtat
     1261 atattatatt ggatgcgggg ttgttcaccg gtttttttcc atcaggcgta ttccattcac
     1321 tctggaatac ctctgttact tttcctataa ctatactgtc tgacattgac actaatttct
     1381 gcaaattgaa tttttccata ataccttctg aactggaaga tattacagta atgggtgttt
     1441 tttcgggagt tgatggtcca atatctgtat tctttttttc tctatcaata caccctgtaa
     1501 caattaccaa tgccattatt gatattatca aaaagatcca aaaaatattg cttcttccaa
     1561 ctttttcttc cggggcatag cgactgccac tttcataata aaacattttc caccgccttt
     1621 ttttgatccc tggtggccac cacacatatc agttagacag aaccacctga atcctgttct
     1681 gagtaaatca taattataaa taaaccttac gaaatctcac tcaataaatg gtgttttctt
     1741 ttctccttat gttatcattc acattatatt tttgaccgat atatatataa attatgtagt
     1801 catcataagt atgaggtata ttaatggcaa ttaaaacaaa gggaatatcc accacatcag
     1861 tatcttcgtg gtgggaagag agaagaaaag tactggcaag taataccaaa ccagtaagag
     1921 taaaaaccaa atctatcaaa ggaaaaccta aaatggtcaa agcgaaagcc aaaccggtca
     1981 gagtgaaacc taaaaccgtc aaagcaaaat ccaaacaggt cagagcaaaa cctaaaacgg
     2041 tcaaggcaaa atccaaacag gtcaggacaa aaccggtaac taaggcaaga acagcatcga
     2101 aaaaaaagaa gatagtaaag acagtgtcta agaccaggaa agtcacctta acaaagaaag
     2161 ctaagaaaaa atgaaatagt tgggtttaat ttcttctttt ttaacgatta cttttgcaga
     2221 tagtgcagac gcccaaattc catcgcccca attcatctga agttggcaaa ctgataaata
     2281 cccttaatta tattttgttt tcagagggat actatcaaat tcaaatcaaa tgactttacc
     2341 aggatactgg cgattgtagc tggaattgca ttcattatga tatctgttgc ctacgggatc
     2401 acatatatat tccagacagg aacaatagta tttcctttcg gaccatatta tttcttattc
     2461 atattctcta tcgggttcgt gattttcact gtttatttcg aacgggagaa aaaagcggtt
     2521 tatccaagag aactaatcgg aggcgcaatg gcatcatcgt gtttgacttt tcttataata
     2581 gcatgcgtcg ccgggatgaa atatatctgg gaaaaagggt tatcaggcat tggtatcgag
     2641 acccttgcct atgcattttc catatccatg atcctgagcc ttattttata ttttgtctta
     2701 ggaaaaatag aagttaaatt cgtccccggg gattttacga gagtactgtc catagtcctg
     2761 ggaatggcaa ttctgattat gataactgtg gtctacggga taaattatat tattaagatc
     2821 gatccatttc cactcaagcc ttatgttctt cttttcatat tcgcaatcgt gttcgtcctg
     2881 gcttctgtct tttatgaaaa aagaggggct ttatatccat ggttcctcct tggcggcgct
     2941 gtggcatccg catgttttgt tttcattatt gcagcaataa ttggcggatt gagatatgtt
     3001 ctcgataaag gctttactgg ccttggtact gatacggtgt tctattcatt gtctatatgt
     3061 attattctca gcatgatcat atatattcag tataatatta taaaagaaaa gctttgattt
     3121 gggataatga tcgaaaaaag gcataaagta gagttgataa aatgggaaat atccggattc
     3181 ttgatgaagc atccataaat aaaatagcag ccggggaagt tatagaacgt cctgcttctg
     3241 tggtcaagga gctgatcgaa aactccatcg atgcaggcgc cgatactatc cggatcgaag
     3301 taacaaaaag cggaaaagac tcgatccgtg tggctgacaa cggctgcggt atgagcaggg
     3361 atgatgcggc gctatcttat gtgaaacacg ccacaagcaa gatcaggaaa attgatgata
     3421 tagaaacagt aagaacaatg ggattcaggg gagaagccct ctcctctatt tctgccattg
     3481 ccagagtaga aatcaccacg aaaacaaaaa atgaattatc aggtacaaaa atcatcatcc
     3541 atggcgggaa acttgtaagt attcttgaga cgggagcccc ggatggaact gccattctgg
     3601 taaaagacct gttctataat acgccggtca ggaaaaaata tcttaaatcc gataattcag
     3661 aacttacccg gataatcgat atagtttcaa ggatagcgat cgggcataat aacatttctt
     3721 tttctctttt caataacggc aaagagttgc tgcgctcacc ggcctccgaa cttcccggca
     3781 ccataataca catttacggg caggaagttg caagagcaat gctgccggtg gatcttgaat
     3841 cccccatcgt aagaataaac ggtttcatct caagaccttc attgatgcga agtagtcttg
     3901 atttccagtc attctatgtc aatgacagga acatcaattc aagagcgatt ggttttgcgc
     3961 taagggaagg ttacggtacc cttatgcccc ggggtcgttt tccgatcgcg gtattaaaga
     4021 tcaatgttga tacgcatgag gtggatgtga atgttcaccc caccaagaac caggtccggc
     4081 tcagccatga gagggatata tgcgatatgg taaagcaggc tgtgaaaaat gcgctttcga
     4141 agaacgatct tattcctgat ataaaaatac cattgcagca gccattactt tacgaaactc
     4201 ccgcagcaga ccctttgcag gtaaaggaaa cgataaacta ttttaaacct tctgccaaag
     4261 acacgcaaag aagactgaga cgatctgata gggcaggcat ggaaatcgtg aaggaacaga
     4321 ataatgagat gcctgatatg aaggttattg gtcaggtgga tagtatttac atactggccg
     4381 gcacaaagaa cggtttgatg atcatagacc agcatgctgc acatgagcgc gtgttttttg
     4441 aacttgtcag ggaatcaaag cgggatgatt cacaggagct gatagttccc gttaacattg
     4501 aacttgattc cagggaaaag gtcctgatca aggataccat tccctacctt gaagagttcg
     4561 gattcaggat atccgaattc gggccggatt catttgctgt gacggctgtc ccgatcgtcc
     4621 tgggaaagct tgaggatgca caaattgtgc atgatataat atccgatatt ctttccgaag
     4681 gaaaaattaa agaagaaacc gggatctttg agagggtaac caaaagcatc gcatgcagaa
     4741 gcgcccttaa ggcaggagct gattgttcta tgtcccagat ggaaaacctc ataatgcagc
     4801 ttttcaggac agataatcct tatacatgtc cacatggaag gccaacgatg gtctctttca
     4861 acaggcagga acttgataaa ttgttccgga gaagctgaac tacgaaaact ttttatatca
     4921 tgagtatcat tattgtgatg cagtaatcca cttgctctac aaaaaaacag gatgtatata
     4981 cgcaggttgt tgttaattac tgcatcaatg tgacgatatg tcaccaaatc acaggattat
     5041 gaacaaaaca atgagatgtg agtagttatg gtcaagaaac ataataatag tcaagaattt
     5101 gaagtaaatg gggatgtcca taaaaaatgg tcggatacct atggcagcgc cacaaaaata
     5161 tgggatgaat cccattcaag acttatgaaa ccatggattg aattaatggg tgagatgtca
     5221 gagaggacaa aagaaatatc tataaatgcg gctccgaaaa attataagga attctataat
     5281 gaatggatga aaacatacga gaattctttt ggcaaattct atcccgtttc gattcccggg
     5341 cccaaagaaa cacttgagaa tttcataaaa atatccggcg aatccaacaa actatacaga
     5401 ttatggatag aagaatcgga agaaaatgcg aaaaggacct ctgaactact tataaatgga
     5461 gctgaccctg ctaaattcaa agagtgctat gaaatgtgga tgaaatccta tgataagatg
     5521 tcaaatgatt ttatcgaaca acccgccgta aaataccaga aagaagtatt tggaaattat
     5581 accgggatac cggatttcta ttcagaaacg ttggtgaaaa tgtccacaat gtggagggat
     5641 tcttttaata agttgtataa tccgtggatt gaaaccatgc agaagctttc catgaatgta
     5701 gacaaaatat ccagaggaaa tgcaggacct gagacatata aagaattctt tgatctatgg
     5761 gcagatacat ataaagaaaa ctttgaaaaa atgtttgacc cacagtcctc aataacatca
     5821 cgggaagcat tagataattt tattgagagc agcgaaataa gcctgaatct ttataaatcg
     5881 tggattttgg ctcttgaaaa tatgcatgaa aaattcaagg atcattctaa gatcacaact
     5941 gatcccgggg cgtataaaga attttttaat ctctgggtga agatgcatga aaaagctctt
     6001 gaagatgtct ttgacaggat gcccatggta tctccggtaa aagagatgat ggagcccata
     6061 agggacgcat gtaagatata tgccaaagca tccataaaaa tgtcaaaaat gtggttggat
     6121 tcgtatacac gcgcagcatg cccggaggta tgagcacgat gcgatcatga atcgaaggag
     6181 agcaggaaat gagagttgaa aacaaggtag taattataac aggttcaggg agcggtattg
     6241 gcagggagac cgccctactt tttggaaaag aaggcgctaa ggtcgtcgtt gcagatgtga
     6301 atgaaaaagc cggtgaggaa acagcagaag agataagaca aaacggaagc gatgcatttt
     6361 ttgcaaagct tgatgtatcc gacagagaac aatcaaaaca agttgttaag gataccctgg
     6421 aaagattcgg gaaaatagat gtactcataa ataatgcggg aattattcag gatgccctca
     6481 tatcaaaaat gagtgaagac cagtgggaca gggttattga tatcgatctt aaagggcctt
     6541 ttaactgcat ccaggcagtt gttgaagcaa tgacaaatca cggaacaggt gagattatca
     6601 atgtatcttc gattgttgct ttgtacggta atattggcca gtcaaattat tcctcggcca
     6661 aagcaggatt gatcggactt accaagaccc ttgcaaagga actgggaaga aaaggaataa
     6721 gggtcaatgc ggtggctccc ggattcatac atactccgat gactgcaacc gtacccgaaa
     6781 aaatacttga aatgatgaaa gaaaaaacgc cgataaaaag acttggacag cctgatgacg
     6841 tcgcatatgc gttattatat cttgcatccg atgaggcgaa tttcgttaat ggcgcagtac
     6901 tttctgtaga tggtggactc ctcctgtaaa tctgaaatga atttgaaaca aaaagaaacc
     6961 aaaatctcaa atcttaaaaa agcgcttgtt aaagcgctga aaatcgatga aaaaaagctt
     7021 gccggcagga taaaagcaat aggttttgaa tgcaggaaat gcgcacagtg ctgcagtgcg
     7081 caatacggcg ataataccgt gatcgttttt ccttcggaaa ttataaggat atgtgataaa
     7141 acaggacttt cccgcgatga gttcgtaata ccgactcctt cacaggacag ggattcccag
     7201 ggaaatatcc atacatttga atgggtcttg aagaaaaact gtgactgcat tttcctgaaa
     7261 aatgacttat gtgaaattta tgaatgcagg ccgtatatct gcagtacgta tccattttat
     7321 ttgcttaacg ggatgcttga gatatcagaa tgtatgggta taggagggaa tatcagcacg
     7381 caagagtgcc ttatgttagc agcgcttctt aaagaaagat acatcaatga aataagggag
     7441 tcgatagctc ttcttgaaag gttcgacgga ttcaatccgg gtaaaagcgg aaatttgtgc
     7501 gtgcatgaca gcgagggcga gcactggatg cattacaatt ataaacatta aactaaatat
     7561 ggcgtactat gaaattactc gctctgtcag accttcacgg cgattattca catattggat
     7621 ctattcttaa aagggccggg gaaattgatg ctgtactcat tgcaggagat ctcacggatt
     7681 tcggacctga tgataaggcc cgtgagctcc tggaaatgtt caaagttcct gttcttgcag
     7741 tgccaggaaa ttgcgataat ccctcacttt tgaaagtcct ggataagaat gaaaatacta
     7801 ttaatctaca taattcactc catactatag gggaattgac attcataggg cttggcggtt
     7861 caaacccaac tccttttaat actccttttg aactatcgga aaaaaagatc ggcgaatatg
     7921 tcgggacttt actgagcgga tcaaaaggaa gaatgatctt attatcccat gcccccccct
     7981 taaataccat ggacaaatta ccgcatggga aca
//
LOCUS       DATGBN010000156         5717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_554742, whole genome shotgun sequence.
ACCESSION   DATGBN010000156 DATGBN010000000
VERSION     DATGBN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554742"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..902)
                     /locus_tag="VKL21_03585"
     CDS             complement(414..902)
                     /locus_tag="VKL21_03585"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMB44885.1"
                     /translation="MIRKCTASDFERIYEIINNGAQAYKGVIPAEYWKEPYMSRAELR
                     RELDDGIEVWGYEEKGKLIGVMGIQHRQSLTLIRHAYVCTSRQNHGIGGKLLSHLREL
                     TAEPILIATWSNALWAVRFYEKHGFRLVSREEGYQLQRKYWSSPEIKINASVVLADEK
                     WL"
     gene            complement(892..1242)
                     /locus_tag="VKL21_03590"
     CDS             complement(892..1242)
                     /locus_tag="VKL21_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44886.1"
                     /translation="MNLKEFLTPDKEKIIMYGAILIISIIGIIIGIYFGGDNPILRLF
                     FIAASFFYLPVLLLPEIVYSIIPSMRPDMDTMTPLQFYFQSGLMFVLGCGYAYLVSCA
                     IIFVRRRRTRDSDS"
     gene            complement(1273..1476)
                     /locus_tag="VKL21_03595"
     CDS             complement(1273..1476)
                     /locus_tag="VKL21_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44887.1"
                     /translation="MKSIHKRIIGLILCLPFIYFLYACGATQPQKLILLCALGAASSL
                     TPFTDIALVIGLYLMITGGGKGE"
     gene            1582..1974
                     /locus_tag="VKL21_03600"
     CDS             1582..1974
                     /locus_tag="VKL21_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44888.1"
                     /translation="MHFSRFGTTINSSAIVIIVVYKQSERFYIVGIEIKEWDKRVNPK
                     MAREYLETYRPTCEYFYLAAKKFSKSTLDLKDIGLFDLTRMKVIKNSEYLYPDTDFRT
                     NLMSRIKNNFKVLSNAVEDPFQRTIGEF"
     gene            2032..2478
                     /locus_tag="VKL21_03605"
     CDS             2032..2478
                     /locus_tag="VKL21_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44889.1"
                     /translation="MKKKVLNFRNIKEQSAKKHIDRKLIFRIRIFYMIGIILTELMLF
                     EVLKGIIGIELSLVGFLLGLFLGFIATRVFIIHWHEERAKVVSRFDTIGIIVMLLYVV
                     ISASRTSLFGHWIHGSVLTAFTYSILAGIMIGRIVGMRLKIKKYTV"
     gene            complement(2586..2927)
                     /locus_tag="VKL21_03610"
     CDS             complement(2586..2927)
                     /locus_tag="VKL21_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44890.1"
                     /translation="MANKVKNSAIDFENNKFKEYLRISPCFFKMCLKTPAEFNKTFKK
                     IFQLTMTKNPHNVELQNCFAIEAQKFKYYDKILEICVYYDIINNEASEEAIEYVLNMN
                     RNIEVYSQAQL"
     gene            complement(3044..3289)
                     /locus_tag="VKL21_03615"
     CDS             complement(3044..3289)
                     /locus_tag="VKL21_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44891.1"
                     /translation="MIQLAVRIIKSLIEGGTLLKNANPKNVNGSSYDLSLGDQYWQNG
                     EQKILDDDNGFIHLKPGDFVIVSSKQQKLKKLTTARP"
     gene            3328..4266
                     /locus_tag="VKL21_03620"
     CDS             3328..4266
                     /locus_tag="VKL21_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HMB44892.1"
                     /translation="MQKKIGLTALVPPELIFACRCSPFDVNNVIPGSKKYPRNKLCAW
                     TAIWQEMLSKREVELDSLIVVAGGDCHNALVDGQKVAMSGVPTHFFFYPFDGDPEYLE
                     SQLHKLVDFLGTIESPEKPGQIKELKKIGKKIDEKRAQGKLSSGDAFRIMISFSDLMG
                     DLDVFREAIYSLKEQKVEMNNRVALIGVPPIYHDFHDVARSLDLQVVFDELPFEFLRH
                     SGTDIHNIASDYCNYTFARPLDFRIRFLQKELEKRHIDGVIHYTQFACHHMLEDEIMR
                     AKLDYPVLTIQGDLPGKTPEQIKLRLEAFREALERA"
     gene            4263..4949
                     /locus_tag="VKL21_03625"
     CDS             4263..4949
                     /locus_tag="VKL21_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HMB44893.1"
                     /translation="MIGLDVGSTAAKKVSIEDGKIEYQITGTHNWKDLITGIDGNDII
                     STGYFRNSVPHRASVTEITAAIYGVRHYFPDADVIVDIGGQDTKIIDVKKNNFIINDK
                     CSAGTGAFLEFVANYFKIELKELGSFHSRAKRIPAINNTCAVFALSEMISQLVAGFTS
                     EEVIAGMHYAFARRISFMVPDAQSLVLIGGTVKNKGMLSALKDILQTEILVPDEPQIV
                     NALGALKYYC"
     gene            4998..>5717
                     /locus_tag="VKL21_03630"
     CDS             4998..>5717
                     /locus_tag="VKL21_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMB44894.1"
                     /translation="MERFKSRNILVVILIGAIIFAIFANEIGFEKFLYLINNVNRPLL
                     LLAVFFNLLNIITFTITWRYLTPGKISLYRLFKFYMAGTFINNITPSFGTAGEPVKAM
                     FLGKETGINKAECFAGVVSVRMLNMFPFLTIGMVGIWLLFSNPAINLGFWAVAGLAFS
                     IGLAFLIFGLIIYFYIRKDQLSSFVHTSIRFFAPFIGLVKKGFDHKSYVAAVDESINS
                     FHGGLKNIRHNKNSLAKAILFS"
BASE COUNT         1749 a         1073 c         1122 g         1773 t
ORIGIN      
        1 cataaattca acctttattt tttgtttaaa taaatatcaa catcatcatc tataggcatg
       61 attatacacg cattaccaga attgtacata atgagtaata tatttttcca ctggaaataa
      121 tggggttatt tcaattgata attgctataa acactgcata aattgattca aaaagaaggg
      181 gggtaactat gtattgctgc ttccttatca caacattaaa ataagcctaa tatattatag
      241 gagtatatga aaaccattaa aatgaagcaa cttaacgaaa aagacgaaga aattgcagat
      301 gcacttattt ctttaggcat gagcaggaat atcgcaatga cgcttacata tatgcaaaat
      361 acgaattctg ctattcataa gcatgtgaat tgttctgtag acttcctgtc ttattataac
      421 cacttctcgt ccgcaagaac cacagatgca ttaatcttga tttctgggga tgaccagtat
      481 tttctctgca actgatatcc ctcttctctg gagaccagtc taaaaccgtg tttttcatag
      541 aatcgaactg cccagagagc attagaccat gtggctatca ggattggttc tgcggtaagt
      601 tcccgtagat gagataataa tttcccaccg atgccgtgat tctgtctgga tgtgcatacg
      661 taggcgtgtc tgattagcgt caagctctgt ctgtgctgaa tgcccatcac tcctatcagt
      721 tttccttttt cttcataccc ccacacctca ataccgtcat ccagttcacg cctcagttca
      781 gctctcgaca tatacggctc tttccagtat tctgcgggta tcactccttt atacgcctgt
      841 gctccgttgt tgataatctc gtagattcgc tcaaaatcgc tggctgtgca tttacgaatc
      901 actgtctctc gtcctccttc tcctaacaaa tatgatagca caggacacaa gatatgcata
      961 accacaacca aggacaaaca ttaaaccaga ttggaaataa aattgcagtg gagtcattgt
     1021 atccatatcc gggcgcatcg aaggaataat ggaatataca atctccggta ataataaaac
     1081 tggaagataa aagaaagatg ctgcaatgaa gaacaggcgt aaaattggat tatcccctcc
     1141 aaaatagatg ccgataatta tgccgattat tgatattata agaatagcgc cgtacattat
     1201 gattttttct ttatccggtg taagaaattc tttcaaattc ataaaattac atccgagaaa
     1261 aatatatttt tcttattcac cttttccgcc acccgtaatc atgagataaa gaccgataac
     1321 taatgcaata tcggtaaatg gagttaaact tgaggctgcg cccaatgcac atagtaaaat
     1381 tagtttttgt ggttgagtag ccccacaagc atacaggaaa taaatgaagg gaaggcacaa
     1441 tataagtcct attatccgct tgtgtatgct tttcatattc attgaaagta attacttatt
     1501 gataaaatat ccgggttgca tccaatactt tcacctcccg cttaccttaa tcttatccca
     1561 taagcctccc tgctgcatct gatgcatttc tccagattcg gaactacaat aaactccagt
     1621 gcgatagtca taattgtagt atataagcag tccgagaggt tctatatagt gggcattgaa
     1681 atcaaggaat gggacaagcg tgttaatcca aagatggcga gggaatatct ggagacatac
     1741 agaccaactt gcgaatattt ttatctggca gcaaaaaagt tctcgaaaag cactcttgat
     1801 ttgaaagata ttgggttgtt tgacctgaca agaatgaaag ttatcaagaa ttcagaatac
     1861 ttgtatcctg atactgattt taggacgaac ctgatgagta gaataaagaa taatttcaag
     1921 gtactatcta atgccgtgga agacccattc cagcggacta ttggggagtt ttaatctttg
     1981 gtgaaccccc tgctttatta agccaagaac aatactgagg atagaacctc aatgaagaaa
     2041 aaagtcttaa attttcggaa tattaaagaa cagtcggcga agaaacacat agacagaaag
     2101 ctcatttttc ggataaggat attttacatg ataggtatca tcttgaccga gcttatgtta
     2161 tttgaggtct taaaaggcat tataggcatc gagctatctc ttgtcggatt tctcctggga
     2221 ttattccttg ggttcattgc cactcgcgtg tttattattc actggcatga agagagagca
     2281 aaagttgttt ctcgttttga tacgattggc attattgtaa tgctattata tgtggttatt
     2341 tccgcatcaa ggacatcgct ttttgggcat tggatacacg gttctgtcct caccgccttt
     2401 acttatagta ttcttgcagg aataatgata ggaaggattg tagggatgcg tttgaagata
     2461 aaaaaatata ctgtctgaga agtttatttc tgtttagaaa aaaggttccc ctgctaagtc
     2521 taatatgaaa atgttcttat atgaactgct gttcaagtca ttgtccggtc ttcatctgca
     2581 agcttttaga gttgtgcttg actatatact tcgatatttc tattcatatt aagcacatat
     2641 tcaatggctt cctctgaagc ctcattattt ataatatcat agtacacaca aatttcaaga
     2701 atcttatcat aatatttaaa tttttgtgcc tcaatagcaa aacaattttg aagctctaca
     2761 ttatgtgggt tttttgtcat cgttaattga aaaatctttt taaaagtttt attaaattcg
     2821 gctggtgttt taaggcacat tttaaaaaaa catggtgaaa tcctaagata ttctttgaat
     2881 ttattgtttt caaaatcgat tgctgaattt tttactttat ttgccaattc gtttacggtc
     2941 ctagcgaaaa attgaccggc tctaatgtca tcgatttcta agtttacttc ggtttttcca
     3001 tcgttaagta ttagtgaaag tgaatttttt tcagtagata tttttatggt cttgccgttg
     3061 tcaatttttt caatttttgc tgtttactag aaacaataac aaaatctccc ggttttaagt
     3121 ggatgaagcc attgtcatca tcaagtattt tctgttcacc attctgccaa tattgatcac
     3181 ccaacgataa atcataagag gatccgttca cattcttagg atttgcgttt tttaataatg
     3241 ttccgccttc aatgagagac tttataattc gaacagcaag ttggatcata caaaaatata
     3301 aattaccagc aaatatattg gatgcctgtg caaaaaaaga tcggtttaac tgctcttgtc
     3361 ccgccggaat taatatttgc gtgcaggtgt tcgccttttg atgtcaataa tgtcatccct
     3421 ggctcaaaaa aatatcccag gaacaaatta tgcgcatgga ccgccatctg gcaggaaatg
     3481 ctttctaaaa gagaggtgga actggactct cttatcgtag tagcgggagg ggattgccat
     3541 aatgcgctcg tagatgggca aaaagttgct atgagcgggg tccccactca ttttttcttt
     3601 tatcctttcg atggagatcc cgaatatctg gaatcacaat tgcataaact cgttgatttt
     3661 ttgggaacta ttgaatcccc ggaaaaaccg gggcaaataa aagaactcaa gaaaatcggg
     3721 aaaaaaattg atgaaaaaag ggcacaaggt aagctctctt cgggggatgc ttttcgtata
     3781 atgatctctt tttccgacct catgggtgat cttgatgtat tcagggaagc tatttactcg
     3841 ctcaaagaac aaaaagttga aatgaataac agggttgcat taatcggggt gcctccgata
     3901 tatcatgatt tccatgatgt tgcccggtcc ctggacctgc aggtagtatt tgatgaacta
     3961 ccctttgaat ttctcaggca ctcaggaacc gatattcata atatcgccag tgattactgc
     4021 aactacacct ttgcaagacc gcttgatttc aggataagat tccttcaaaa agaacttgaa
     4081 aaaagacata ttgacggagt aatacattat acacagttcg cctgccacca catgcttgaa
     4141 gatgagatta tgagggcaaa gcttgattat cctgtgctga ccatccaggg agacctgccc
     4201 ggcaaaacac ctgagcagat aaaattgagg cttgaagcct tcagggaggc gcttgagagg
     4261 gcatgatagg gcttgatgtt ggcagtactg ctgcaaaaaa agtttcaatt gaagatggga
     4321 aaatagaata tcagataaca gggacccaca attggaaaga cttgataaca ggaattgatg
     4381 gtaatgatat tatttcgacc gggtattttc gaaattcagt cccgcatcgc gcttctgtaa
     4441 cagagataac agctgcgatc tacggggtca ggcactattt tcctgatgcg gatgtgatcg
     4501 tggatatcgg gggacaggac acaaagatca tcgatgtcaa aaaaaataat ttcataataa
     4561 acgacaaatg cagcgcaggc acaggcgctt ttcttgagtt cgtggcaaat tattttaaga
     4621 tcgagctaaa agaactgggc tcattccaca gtagagctaa acgaatccct gcgataaata
     4681 acacatgcgc agtatttgca ctttccgaaa tgatctcaca gcttgtagcg ggtttcactt
     4741 ctgaagaagt tatagcaggg atgcattatg catttgcgcg cagaatctcg tttatggtac
     4801 ctgacgcaca atcacttgtc ctgatcggag gtacagtaaa gaataaagga atgttgtcag
     4861 cgctcaagga tatcctccaa acagaaatac tggtaccgga tgagcctcag atcgtgaacg
     4921 ccctgggtgc gctgaaatat tattgctgag tacccacaat tcttattaag tgtgagagca
     4981 agtatcggtt agatttcatg gaaaggttca aatctagaaa tattctggtc gtaattctaa
     5041 taggggcgat aatttttgca attttcgcaa atgagatagg atttgaaaaa ttcttatatt
     5101 taataaataa tgtcaataga cccttacttc ttcttgcagt atttttcaat ttattgaata
     5161 tcataacttt cactatcaca tggcgatacc tgacacctgg aaagatcagc ctttacagat
     5221 tatttaaatt ctatatggca gggacattta tcaacaacat caccccatca ttcgggacag
     5281 caggtgaacc tgtaaaagcc atgtttctgg ggaaagagac aggaataaat aaagccgaat
     5341 gttttgcggg agttgtttcg gtcaggatgc tcaatatgtt cccattcctg accatcggga
     5401 tggtgggtat ctggttattg ttctccaatc ctgcgataaa tcttgggttc tgggcggtcg
     5461 caggattggc gttctctatc ggacttgcgt ttctaatttt tggccttata atctattttt
     5521 atataagaaa agatcagcta tcttcatttg tacatacatc cattcgattt tttgccccgt
     5581 ttatcgggtt ggtgaaaaaa ggttttgatc ataagtcata cgttgctgca gttgatgaat
     5641 caattaactc attccacggg ggtttaaaga atatacgtca taataagaat tccctggcaa
     5701 aagcaattct gttctca
//
LOCUS       DATGBN010000157         7146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_361300, whole genome shotgun sequence.
ACCESSION   DATGBN010000157 DATGBN010000000
VERSION     DATGBN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361300"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VKL21_03635"
     CDS             complement(<1..1296)
                     /locus_tag="VKL21_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44895.1"
                     /translation="MWANIIFMGNVARGIVRSKNRFIKSLMLTAAIVVILISIPPVFA
                     YTNNTTKCISCHNDTTYPFDTDGDGVAAPYKRPHNNLIMCESCHLPDPHIVKFIQPDG
                     GYGGKSTAASCPECHQNGIPSQNNSNFTSAPLITPVLRHSQNILNGSIWGNYWTNTSL
                     NTACKYCHDNTLHNITAFGRILLLFPGYQRYGELANNYTCAGCHYKNDPKWNQMNLTF
                     TSKGLPIPPEITNGTNWNGKSFKYYNHSLQIYDDKICRTCHGSFLSANANMTEFVHNV
                     AEGAGGEECLACHVNDLGKFPGINVTLFGRHKNVNTTDGDLGTLKNSDCRTCHYDFSN
                     MSDVQTYTCNYCHDQGNFSAPMIKNHQPNGVNITTAAYCSTCHKNSINRYAFSENASV
                     AHYGTNTTLIKPTVNQSAVPRFGFATGSEASAYNRDCNNC"
     gene            complement(1394..3169)
                     /locus_tag="VKL21_03640"
     CDS             complement(1394..3169)
                     /locus_tag="VKL21_03640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMB44896.1"
                     /translation="MKKINKFVESNQLPLLIFFISMFLFISFAGTRLFFSDEGIILDQ
                     FYNLINGSLALKIAKINVEKGIFMTIGTNLYGKFSYSLLILSLPTYYILKIIDHIYSA
                     HLFILQLWAVIGGIVVFLSGKIRNIKHPKVWGIIAYLILAITNQYFFKSIYFPKWGEL
                     LSIEFTNIIISSFLILIIYLFFRNFFSNKIALFASFFIMFATPIPFYAITLKHHNLTL
                     LLTLLSFYFFYKSYEKKDNKFIYLAYISAGLCLWTRILDGSVLLVSLLIIDFVIFRRS
                     IKYILSISIVILISLLPFFVFNYLILGDPFSIIERTPLTSEPVSLITAKEFIVLDVNQ
                     NSARQVQLLNDLGYTWNGKIKGYWFEVIGYSLFSKLINTFGIFLVSPFLIAALGFIVY
                     GVKGKIKLNPVDKLLGLYTMLLFGSYALLQIFFDIRSLISIITDTPSGLDYRYLLILY
                     IVFLYFCLRMDNVRELIENNLKTILVYYGIIFIIMLSYFIIEFPVLFIGIYYYMAIIT
                     SIFLVILISLRPFISNKKNKKNKKALLNRSLSFIMALSLAEASFFLLFYYWVVTMTYI
                     SPSQNYTILPVMGKIINWMYQIIIY"
     gene            complement(3166..3918)
                     /locus_tag="VKL21_03645"
     CDS             complement(3166..3918)
                     /locus_tag="VKL21_03645"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMB44897.1"
                     /translation="MYIVVVHYHIFFYNHMYKTSIWHHLINSVLIRKLFFTNKKGKRG
                     EFYLERFREHKQSRLSRNVVVQLLPVLVILALFYVLTSQNLYFGTVLSGSMEPTFKRG
                     DLVLMQSLYAEPRIGDIIMFSPVKGMEPITHRIISIDKYGYIRTKGDANPKEDSWSFK
                     KGYIIGKSVIIGKKPVVIPGLGSTLVSRAENFTIIKTVTKESGILSLFQEFRTIAPLL
                     IFFMLIIYIFKVFETSSEEKHRFTRDNRKKPK"
     gene            complement(3927..4514)
                     /locus_tag="VKL21_03650"
     CDS             complement(3927..4514)
                     /locus_tag="VKL21_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44898.1"
                     /translation="MKKNQLIILMIAVIAIGLFALPSTFTYAGQMHTFRQIDPTSPAT
                     INEFCEKCHTGDVQAQLDASDKGLYQGNGIIHATIGCAGCHQITRDGGPNGYNNGQFG
                     YDPSLTETGAGVSIEHAARIPSCLDCHIAGAPLIQWGDAGNEIRAPTEAHKNFKFATD
                     NDLECIGCHTDVARTGSVSSTFQPAPLSYGGLTIG"
     gene            complement(4622..5074)
                     /locus_tag="VKL21_03655"
     CDS             complement(4622..5074)
                     /locus_tag="VKL21_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44899.1"
                     /translation="MKYLKVVLLILTVLLFSGCIEEITGRFMKVDVMYVNLSVVNEDN
                     KTLIKDIQAKRGELPKINSPGFVAPDKFPGIYVKIKQPYNISKPDLVKDVGVPNGLDF
                     NGTGNYSFTVQLYENEFNKTLPVYIYSEIIDNKSQRLARTIIGVNLTE"
     gene            5152..6078
                     /locus_tag="VKL21_03660"
     CDS             5152..6078
                     /locus_tag="VKL21_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44900.1"
                     /translation="MNERNRTELLILFGCMIFVVSILSGIELSNKLNKPEFCSPCHEM
                     QTYYTSYLNPKNGSILMDHELTCIQCHSGRNIYDAKKQVLIKVIAYDLNLSGAFISKK
                     LEPDCSKCHSLPSTPVHKNANLTNCAYCHWAHSSAEGSITSNNSLLPMIPYGPHMNQT
                     CVKCHSTTYQIPRCINCHAGHGEQKLENNLCLICHKDPHVPTIPGILQNNTVNFTKEL
                     PFSACQPCHEKEYSEIIDSGSLHKDMETCSLCHLKHGTKPNCSQCHKAMKVQRHLDNL
                     QCGNCHTPDRVITCEECHGRSHGWSASTATVI"
     gene            complement(6382..>7146)
                     /locus_tag="VKL21_03665"
     CDS             complement(6382..>7146)
                     /locus_tag="VKL21_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44901.1"
                     /translation="KFTVHSTDVDGDTLTFDTDATKGSLDSDTGAYSWKTASGDAREY
                     TWYFKTMDNYGGVDTETIVVTVDPAPLPVPPAPVNLDVTQGNFWIEYTWKPGTGTKTD
                     SYKLKVNGVSSTTSLTSRRDTVEPHGSSEITVWAYNNSGGQSLNSVSRSTQVQNNKPV
                     QSSIGNKNVLEGDTLKFTVHSTDVDGDTLTFDTDATKGSLDSDTGAYSWKTTSGDAGE
                     YTWYFKTMDNYGGVDTETIVVTVDPAPLPVPPAPES"
BASE COUNT         2150 a         1402 c         1338 g         2256 t
ORIGIN      
        1 gcagttatta caatccctgt tataggctga cgcttctgag cctgtcgcaa aaccaaatct
       61 tggaacggcg ctctggttga ccgtgggttt gatcagggtt gtgttcgttc cataatgtgc
      121 tacgcttgca ttttctgaaa atgcataccg attgatgctg ttcttatgac aggtcgaaca
      181 gtatgccgct gttgtaatat tcacaccgtt tggctgatgg ttctttatca tcggggctga
      241 gaagttgccc tggtcatgac aataattaca ggtgtatgtt tgaacgtcgc tcatgtttga
      301 gaaatcataa tgacatgtcc tgcagtcact attttttagg gtacctaaat ctccatccgt
      361 ggtgttaaca tttttatgtc ttccaaataa tgttacatta attcctggaa attttcccag
      421 atcatttaca tgacatgcca gacattcttc gcctccggcg ccttcggcga cgttatgcac
      481 gaattcagtc atattagcat ttgcacttaa gaatgagcca tggcaggttc tgcaaatttt
      541 atcgtcgtaa atttgaagag aatgattata atatttaaaa gattttccat tccagttcgt
      601 tccattggtg atttccggag gtattggtaa tcctttcgaa gtgaaagtaa ggttcatctg
      661 gttccattta ggatcgttct tatagtggca gccagcgcag gtataattgt ttgcgagttc
      721 accgtatctt tgataaccgg ggaataagag caatattctt ccaaatgctg taatattatg
      781 aagtgtatta tcgtggcagt atttacacgc ggtattcagc gatgtatttg tccagtaatt
      841 accccatatt gaaccgttca aaatattttg gctgtggcga agtaccgggg ttattaaggg
      901 ggcgctggta aaatttgaat tgttctgcga agggattcca ttttgatggc attcaggaca
      961 tgaagctgct gttgatttac cgccatagcc gccatctggc tggatgaatt taacaatatg
     1021 agggtctggt aagtgacagg attcacacat gataagatta ttgtgcgggc gtttatatgg
     1081 cgcagctact ccatccccat ctgtatcgaa tggataagtt gtatcattgt gacatgaaat
     1141 acattttgtt gtattgttcg tgtaagcaaa cacgggaggt attgaaatta gaatgacaac
     1201 gatcgcagct gtcaacatca atgatttaat aaatctattc tttgacctta ctatgcctct
     1261 ggcaacattt cccataaata ttatattagc ccacataaaa cctatcacat ctatccaatt
     1321 atattactat aaactttcta ttatcataaa ggtttcgtaa tttttatagt agaggagttt
     1381 atttatatta tttttaatat attattattt gatacatcca gtttataatt ttccccataa
     1441 ccggaaggat cgtatagttt tgtgaaggcg agatataggt cattgtaact acccagtaat
     1501 aaaaaagcag gaaaaaagaa gcttctgcaa gagataatgc cataataaag gacaatgatc
     1561 tgttaagtaa agctttttta ttttttttat tttttttatt cgagataaaa ggtcttaatg
     1621 aaatcaatat aacaagaaag attgatgtaa ttattgccat atagtaataa atacctatga
     1681 aaagaacagg aaattcaata ataaaataag ataacataat tataaatatt atgccataat
     1741 atacgagtat tgtttttaaa ttgttttcaa tgagttcccg cacattatcc attctcaggc
     1801 aaaaataaag aaagactata tacaaaatca agagatacct gtagtcaaga ccactcggag
     1861 tatctgttat tatagaaata agagagcgaa tatcaaagaa aatctgcaat agggcatatg
     1921 acccaaataa tagcattgta tataatccca gcaatttatc taccggattc aacttgattt
     1981 tcccttttac gccatacact atgaacccaa gcgctgctat caagaacggg cttaccaaaa
     2041 aaataccaaa tgtattgatc aattttgaaa acaatgaata tcctatgacc tcaaaccaat
     2101 aacccttgat tttcccattc catgtatatc caagatcatt taaaagttga acttgccttg
     2161 cactattctg atttacatca agtactataa attcttttgc cgtgatcaat gatacgggtt
     2221 cacttgttag cggagttctc tcaatgatcg aaaaaggatc gcccagaatc aaatagttaa
     2281 aaacaaaaaa tggcaatagc gatatcagga ttactattga aatagataaa atatatttaa
     2341 tgctgcgtct gaaaattaca aagtcaatta tcaggagtga tacaagaagc acggagccat
     2401 ccaaaattcg cgtccataaa caaagtcctg ctgatatgta tgcaagataa atgaatttat
     2461 tatccttttt ttcataactt ttataaaaaa agtagaaact taataatgtt aagagtaaag
     2521 taaggttgtg atgtttcaat gtaatggcat aaaacgggat cggtgttgca aacataataa
     2581 aaaaggatgc aaatagagca atcttattac taaagaaatt cctgaaaaac aggtaaatga
     2641 taagaataag aaaagaagag atgataatgt tagtaaattc aatggataac aattctcccc
     2701 acttcggaaa ataaatagat ttaaaaaaat attgattggt gattgcaagt attaaatacg
     2761 ctatgatccc ccatactttt ggatgcttta tgtttcggat ttttcctgag agaaaaacaa
     2821 cgatcccgcc gatcactgcc cataattgca ggataaaaag atgagcgcta tagatatgat
     2881 ctattatttt caatatataa tatgttggta aagataaaat aagtaatgaa tagctgaatt
     2941 tcccatacag attggtccct attgtcataa atattccttt ttccacattt attttagcta
     3001 tctttaaagc cagggagccg ttgattaaat tataaaattg gtctaatatt attccttcat
     3061 cagagaaaaa caatcgtgtt cctgcaaaac tgataaataa gaacatcgaa atgaaaaaga
     3121 tcagtaaagg aagctgattt gattcaacga acttatttat ttttttcatt ttggtttttt
     3181 tctattatcc cttgtgaacc tgtgtttttc ttcactacta gtttcaaata ctttaaagat
     3241 atagatgatc agcataaaga aaatcaataa aggcgcaatg gtacggaact cctgaaatag
     3301 agaaagaatt ccactttcct ttgttactgt ttttattatt gtaaaatttt ctgccctcga
     3361 taccagtgtg gagcctaatc ccggtatgac tacaggtttc tttccgatta ttacggattt
     3421 ccctattata tatccctttt tgaaactcca actatcttct tttgggttgg cgtcaccttt
     3481 tgttcttata tatccatatt tgtcaatact tattatcctg tgtgttattg gctccatacc
     3541 ctttaccggc gagaacatga tgatatctcc aattcgcggc tccgcgtata aagattgcat
     3601 tagcaccagg tcaccccttt taaaagttgg ctccatactt ccgctgagca ctgttccaaa
     3661 atataggttt tgactggtca gaacatagaa taaggccaat atgaccagaa caggcagaag
     3721 ttgcaccact acattcctgc taagtctact ctgtttgtgt tcacggaagc gctctaaata
     3781 gaattcgccc cttttccctt ttttgtttgt aaaaaacaat ttcctgatca gtacactgtt
     3841 aattaaatgg tgccaaattg aagttttata catatgatta taaaaaaaaa tgtggtagtg
     3901 aactaccaca atgtacatat tctgttttat ccgatcgtta agcctccata tgagagaggt
     3961 gctggctgga atgttgacga tacactaccg gttcttgcca catctgtatg gcatccgata
     4021 cattcaaggt cgttgtcggt ggcaaatttg aagttcttgt gtgcttcagt gggtgcccta
     4081 atttcgtttc ctgcatcacc ccattggatt aatggtgctc cggctatatg gcaatccaga
     4141 caggatggta tccttgctgc gtgttctatt gacactcctg ctccggtttc agtcaaactg
     4201 gggtcgtagc caaactgtcc attattatag ccatttggcc ctccatctct ggttatctga
     4261 tgacaccccg cgcaacctat ggtagcgtgg attatgccat ttccctgata taaccccttg
     4321 tcgctggcat ctaattgtgc ttgaacatcg cccgtatggc acttctcgca gaattcattg
     4381 attgtagcag gtgaagtggg atcaatctgt ctgaatgtgt gcatttggcc tgcatatgta
     4441 aatgtgcttg gtaatgcaaa caatcctatc gctataactg cgatcattaa aattattaat
     4501 tgattctttt tcattttttt tcacctcctt tcctatccat attatttccc acccaatttg
     4561 ggtctaattt gggtatatgt tactatagtc ttttattcta tataaatata tctctgggat
     4621 ctcattcggt taaattaaca ccaattattg ttcttgcaag cctctggctt ttattatcaa
     4681 tgatctcact gtaaatgtat acgggcaggg ttttgttaaa ttcattttca taaagctgta
     4741 cggtaaagga ataatttcct gttccattga aatccagtcc atttggtaca ccaacatctt
     4801 ttacaagatc tggtttagaa atattgtatg gctgttttat ttttacatat attccgggaa
     4861 atttatccgg tgcaacaaat ccaggcgaat taatcttggg gagttcaccc ctctttgcct
     4921 ggatgtcctt gatcagcgtc ttattgtcct catttacaac gctcaaatta acgtacatca
     4981 catccacttt catgaatctg cctgttattt cttctatgca gcctgagaat aaaagaactg
     5041 tcaatataag aagaactact tttaaatatt tcatgatttc aaaatagaaa gacttacatt
     5101 taacccttat ggtttattac ctactattga tataagtaat actattctct gatgaatgaa
     5161 agaaacagaa cagaactact tattctcttt ggctgcatga tatttgtggt cagcattcta
     5221 tccggcatag agttaagcaa taagctgaac aaacctgaat tctgcagccc atgccatgag
     5281 atgcaaacat attatacttc ttatctaaat ccaaaaaatg gctcaatatt aatggatcat
     5341 gaactaacct gcatccagtg ccactccggc aggaatatat atgacgctaa aaaacaagtg
     5401 ttgatcaagg tcattgctta tgatctcaac ctttcaggcg cttttatatc aaaaaaactg
     5461 gaacctgatt gctcaaagtg tcattcactt ccttccactc ctgtccacaa aaatgcaaat
     5521 ctgacaaact gtgcctactg ccattgggcc cactcgtctg ctgaggggtc aataacctct
     5581 aataattccc tgcttcccat gatcccttac ggtcctcaca tgaaccagac ctgcgtaaag
     5641 tgtcacagca caacctatca gattccccgg tgcataaact gccatgcagg acacggtgag
     5701 cagaagcttg agaacaatct ctgtctgata tgccataaag atccacatgt ccccactata
     5761 ccaggaatac tgcaaaataa taccgtaaat ttcaccaaag aattaccttt ttctgcctgc
     5821 cagccctgtc atgaaaaaga atattccgaa attatagata gcggctcact gcacaaagat
     5881 atggagacat gcagtttatg ccatcttaaa catggaacga aaccaaactg tagtcaatgt
     5941 cataaagcca tgaaagtaca aagacattta gataatcttc aatgtggtaa ttgccacaca
     6001 cctgatcgcg taatcacatg tgaagaatgc catgggaggt cgcacgggtg gagtgcttct
     6061 actgctacag ttatctgaaa aaataattga gcatattatt gataattaat agtcgcctca
     6121 tacaaataga tgtttgatcc tgcgttgctc tggtattgac tcatttagca aaactgaaag
     6181 taagtgtatt cccattcaat gctaaatatt tattcttgtc agaatgagaa gacatgcctg
     6241 ttattctgta cctaggttga ccgggatacg gaattcaggc tctgccctcc ggaattgttg
     6301 taagcccaga cgagttgcca gtctttgttc caatgcctgg cttccaggtg tattcaaccc
     6361 agaaattacc ctaaagtgac gtcaagattc aggagcagga ggaacgggca atggcgcggg
     6421 atctacagtg acaacgattg tttcggtgtc aactccgcca taattatcca tggtcttgaa
     6481 gtaccaggta tattccccgg catcgccaga ggtagtcttc caggaatatg ctccggtatc
     6541 ggaatcaaga cttccttttg ttgcatcggt atcgaaggtc agggtatcgc catcaacgtc
     6601 agtggaatgg acagtaaatt tcagggtatc tccttccaga acattcttgt tcccgatcga
     6661 ggactggaca ggcttgttat tctgtacctg ggttgaccgg gatacggaat tcagactctg
     6721 gcctcccgaa ttgttgtaag cccagacggt tatttcgctc gagccgtgcg gctcgaccgt
     6781 atccctgcgg gaagtaaggg atgttgtaga ggagacgccg ttaaccttca atttgtagct
     6841 gtcagtcttc gttccagttc ctggcttcca ggtgtattct atccaaaaat taccctgagt
     6901 aacgtcaaga ttcacagggg caggaggaac gggcaatggc gcgggatcta cagtgaccac
     6961 gatggtttcg gtgtcaactc cgccataatt atccatggtc ttgaagtacc aggtatattc
     7021 tctcgcatcg ccggaggccg tcttccagga atatgctccg gtatcggaat caagacttcc
     7081 ttttgttgca tcggtatcga aggtcagggt atcgccatca acgtcagtgg aatggacagt
     7141 aaattt
//
LOCUS       DATGBN010000158         6033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_244617, whole genome shotgun sequence.
ACCESSION   DATGBN010000158 DATGBN010000000
VERSION     DATGBN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244617"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VKL21_03670"
     CDS             <1..313
                     /locus_tag="VKL21_03670"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB44902.1"
                     /translation="IQDVFTDIIQSIIRNYTPQKIVLFGSYARGEAHEGSDIDLMVIK
                     ETSKRFIDRIADIIKLNNTLIPLEPLVYSPLEFETMKKEKRDFIMTIEEEGIEIYTNP
                     S"
     gene            318..2846
                     /gene="gyrA"
                     /locus_tag="VKL21_03675"
     CDS             318..2846
                     /gene="gyrA"
                     /locus_tag="VKL21_03675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMB44903.1"
                     /translation="MTDPEPKDPQQQLNIEKITPVNIEDEMKRSYIDYAMSVIVGRAL
                     PDVRDGLKPVHRRILYAMNEQGMTHDKPYKKSARIVGDVLGKYHPHGDIAVYDSLVRM
                     VQEFSLRYPLIDGQGNFGSIDGDSAAAMRYTEVRLGKIADEILGDIDKETVDFVPNYD
                     DSLKEPSVLPCKLPNLLINGSTGIAVGMATNMPPHNLGEVIDGITMVIDKPDVDTREL
                     NSIIKGPDFPTAGFIYGRQGIIDAYTTGRGSVKMRARAIIEEVKNKETIIVNEIPYQV
                     NKAKLIEDIAGLVRDKKVAGITDLRDESDKDGIRIVIEISRQSQANIILNQLYSHTQL
                     ETTFGVINLALVDGRPMVLPLKEIIVQFISHRKHVIIRRSQFELKKAQARAHILEGLI
                     IALDHIDEVIKLIRASKTPDEARDGLVAKFSLSVEQAKAILDMRLQRLTGLEREKIDT
                     EYGELQKTINWLKELLASEIKILALIKEELADIKARFSNKRRTEIIESTGDLVTEDLI
                     PKEDDVITITNSGYIKRIAVDAYKQQRRGGKGVMGMETKEEDFVSDLFIGNTHDYLLF
                     FTDKGKVYWLKVYGIPEAGRQARGTAIVNLLQIEQDEKINAYIPISKFDEIHYLLMVT
                     KKGTAKKTQLTDFSNPRKTGIIAISLDEGDKLVSVKLTDGTKEMVIGTKHGKAIRFIE
                     SNVRDMGRSAYGVRGVRLVGDDEVVSMAVVEEGATLLTVTENGFGKRTVFDEYRTSNR
                     GGQGVITIDVNVRNGNVVDIRTVREDEEIMITTSNGIIIRVPASGIRVQGRNTQGVKI
                     MNVGEKDKVVGVATLAKEEEAAEAVQEKLEEVEEAKSEEKLEKI"
     gene            3109..3255
                     /locus_tag="VKL21_03680"
     CDS             3109..3255
                     /locus_tag="VKL21_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44904.1"
                     /translation="MTRIDMSRIVRKYEKYLKFKTKKGEIPMAPGEWLWHNLDKLDLD
                     KITK"
     gene            3741..4373
                     /locus_tag="VKL21_03685"
                     /pseudo
     CDS             3741..4373
                     /locus_tag="VKL21_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            complement(4786..5160)
                     /locus_tag="VKL21_03690"
     CDS             complement(4786..5160)
                     /locus_tag="VKL21_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator protein"
                     /protein_id="HMB44905.1"
                     /translation="MKTIKMKQLDKKDEEIADALIILGMSKNVAMTLAYMQSTNSATS
                     IELERSARLRQPEVSIVMKQLKERDWINESEEKKAGKGRPYKIYSFKVGFKDIIAQLE
                     KQQRKAVDETQEKIEKLKGVGK"
BASE COUNT         1881 a         1159 c         1391 g         1602 t
ORIGIN      
        1 tattcaagat gtatttacag atatcatcca gagcataatc cgtaactata ctccccagaa
       61 gattgtgctt tttggttcat acgcaagagg agaagcgcat gaaggaagcg atattgacct
      121 tatggtaata aaagaaacct caaaaaggtt catcgataga attgcagata taataaaact
      181 aaataataca ctgattcccc ttgaacctct tgtatattca cccttagaat ttgaaacaat
      241 gaaaaaagag aaaagagatt ttataatgac aattgaagaa gaaggaattg agatttacac
      301 taatccgagc tgataccatg accgaccctg aaccaaaaga cccacaacag caacttaata
      361 tcgaaaagat cacccccgtg aacatcgagg acgaaatgaa acgctcgtac atcgattatg
      421 ccatgagcgt tatcgtgggg cgcgccctcc cggatgtcag ggacggcctg aagccggtgc
      481 accgccgcat cctgtatgcc atgaacgagc aggggatgac acacgacaaa ccctataaga
      541 aatcagcaag gatcgtggga gacgttcttg gtaagtacca cccgcatggc gatatcgctg
      601 tttatgattc cctggtaagg atggtacagg agttctcttt acgatatcct ttgatagatg
      661 gacaaggaaa ttttggaagt attgacgggg attcagccgc agccatgcga tatactgaag
      721 ttcgccttgg aaaaatagcc gatgagatcc tgggcgacat cgacaaagaa acagttgatt
      781 tcgttcccaa ctacgatgac tcattaaaag agccctcagt tcttccatgc aagctaccca
      841 accttctcat caatggttcg acaggtatcg ccgtgggcat ggccaccaat atgccgcccc
      901 ataatcttgg cgaggtgatc gacggaataa caatggtcat cgataaaccc gacgttgata
      961 ccagggagct gaatagcatc atcaagggtc ctgatttccc cacagcaggc tttatctatg
     1021 gccgccaggg gataatcgac gcatacacga caggccgtgg atcggtaaag atgcgcgccc
     1081 gggcaataat cgaggaagtg aagaataagg aaaccatcat cgtcaatgag atcccttacc
     1141 aggtgaataa ggcaaaattg atcgaagata tcgcagggct tgtcagggac aaaaaagttg
     1201 ccggaataac cgatcttcgc gatgaatcag ataaagacgg catcaggatc gtcattgaga
     1261 tatcgagaca gtcacaggcg aacattattt tgaaccagct ttattcgcac actcagcttg
     1321 aaaccacttt tggagttatt aacctggctc ttgtggatgg gcggccaatg gttcttccat
     1381 taaaagaaat aatcgtccag tttatttcac acaggaagca tgtcatcata cgaagaagcc
     1441 agttcgaact taagaaagcg caggcaagag cccatatact cgaaggtttg ataatcgcgc
     1501 ttgaccatat cgatgaagtg atcaagctta taagggcctc aaagacgcct gatgaagcgc
     1561 gcgatggtct tgttgcaaag ttcagtctca gcgttgagca ggcaaaagct atcctggaca
     1621 tgaggcttca aaggctcacg ggtcttgagc gcgagaagat cgatactgag tacggtgagc
     1681 tacagaagac aataaactgg ctcaaagaac tcctggcaag cgaaataaaa atacttgcgc
     1741 ttataaaaga agaacttgca gacatcaagg cccgcttttc gaataaaagg cggacagaga
     1801 taattgaaag tacaggcgat cttgtgacag aggaccttat tcccaaagaa gacgatgtca
     1861 ttacgataac caatagcggt tatataaaac gcatcgcagt ggatgcatat aaacagcagc
     1921 gccgcggcgg taagggtgtc atgggtatgg agacaaagga agaggatttc gtaagcgacc
     1981 tctttattgg caatactcac gattacctcc ttttcttcac ggacaaagga aaagtctact
     2041 ggctgaaagt atacggaata ccggaagcgg ggaggcaggc gcggggcact gccatagtca
     2101 accttttgca gattgagcag gatgaaaaga tcaacgctta cataccgata agtaagttcg
     2161 atgaaataca ctatcttttg atggtcacaa agaaggggac tgcaaagaag acacagctta
     2221 ctgatttctc aaatcccaga aaaacaggga ttattgccat ctctcttgac gagggggata
     2281 aactggtatc tgtaaagctc acggatggca caaaggaaat ggtaatcggc acaaagcacg
     2341 ggaaagccat ccggtttatc gagagcaatg tgcgcgacat gggccgcagc gcatatggtg
     2401 tacgcggcgt aaggcttgta ggtgatgatg aagtggttag catggcagtt gtggaagaag
     2461 gggcaacact tctcacagtg accgagaacg gattcggtaa acgcactgta tttgatgaat
     2521 accgaacaag caaccggggg ggacagggag tgataaccat cgatgtcaat gtcaggaatg
     2581 gcaacgtggt ggatatcagg accgtcaggg aggatgagga gataatgatc actacctcaa
     2641 acggtataat catccgcgtt cctgccagcg ggatccgtgt gcagggaagg aatacgcagg
     2701 gtgtaaagat aatgaatgtg ggggagaagg acaaggtcgt tggcgtggct acgcttgcga
     2761 aagaggagga ggctgcagag gcggtgcagg agaagctgga agaggtggaa gaagcaaaaa
     2821 gtgaggagaa gcttgaaaaa atttaatatt ttgggttata attatgctta tgtaaaaaaa
     2881 gataactttt ataggacttt aaaatttaat cttatagaga tgatttgatc cggcgcgccc
     2941 gatattgcgc gaaaatgaga aagcaacgtg cgatactttc gcaactgtct aatccttatt
     3001 atgtagtgcc taaaaacgag gctaacgata gctatttatc tagagaaatc aatataaatt
     3061 tgtcctcgaa cgagggaatt tcccggatga gataattctg agtgagttat gacgcgaatt
     3121 gatatgagta ggattgtacg gaaatacgaa aaatatctca aatttaagac taagaaaggt
     3181 gaaattccta tggcgcctgg ggaatggtta tggcataatc ttgataaact tgaccttgat
     3241 aaaattacga agtgaagctg aatggagatc agaaatatga caaaatgtgc attaggcggc
     3301 ggcgtgcgaa agtttcgggt agtggggcga tgatggcgca gcgttctggt ttagtacacc
     3361 ctgtatgaac tgaatgtatc cacgtatctc ccatgcaagc cggaaaatgc cgaaatcagc
     3421 gccgatgtga agcatagata tgactattta tcgtaaagaa tataataatt atacatgggc
     3481 gtgattcggc tcggctgttc tggatgggat tatagagatt gggctgacgt gttgtataag
     3541 accacggaag agtcaaaact ccgtgtatat tcacagattt ttaatactgc cgagattaat
     3601 tccacgttct acagttatcc atcacatggt attgtctttg gctggtcaaa gtacacgcct
     3661 cctgatttca agtttgcggt gaagttgaac cccataataa cacactaaaa gaggctggac
     3721 atttcaaagg ggtcggagat gatctaaatc gattcacgga actgatgaaa ccgcttcaag
     3781 atactgggaa gctcgcctgc atcctgatcc agcttcctcc gggtatgaag ttcagaaaag
     3841 accggataga agcgtttctg aatttactac cctctgaatg tcggtttgca cttgagtaca
     3901 ggaatgagac atggctcact aacgaggcgc atgaattgct ttctgattac aatgttaccg
     3961 ctgtgattgt ggatgaaccg ctacttccgc cagatgtcag gatgacctcg gattttgctt
     4021 atttacgctg gcatgggagg ggcgagaaaa tgtggtacaa ctatcgctat tcaagggagg
     4081 aacttgaaga atggataccg aaaatcaagg aattgtcgca gaaagctgag gtttatggct
     4141 acttcaacaa ccattaccac ggatatgtgc cagagaactg catagacgta ttgaatatgc
     4201 tggaagtagc cactcctgaa caaaaagagg cgaggctgca tatttcagat tattggaaag
     4261 gcaaaggaaa agggaaagtt gtggcaaaga cgctaaatga ttttcttgag ccggaaaaag
     4321 aggatgtaat gactttgctc tcagtacata tcgataacga gaattgccgc taatctatcc
     4381 agacaaagca tatcctggag tacgcagaat agccaattgg ttcaacggat atatgcaaac
     4441 tcttggtggg caaaaaatct ggaaatcctt aaacaattaa agtttcagga gcagaaaata
     4501 gagagatttg aataaattca atgatgtgct gcgagatgct agatatggat acaaccatag
     4561 gccgaattct gcatttggtt acaggaatcc tttagaagtt atggctacaa aaatatcaaa
     4621 tttaccccga caggtcgctg gacactaacg ctaaggattg aagaaaatct ctgtaatttt
     4681 cagccattct aatattttta tgttgtgcct catctaattg tcttttggct ctactgatta
     4741 ttttgtgttt aattttattc attatattat ttagtaagag tgaaactatt tccctacccc
     4801 cttcagtttc tcaatcttct cctgtgtctc atcaaccgct tttctttgtt gtttttcaag
     4861 ctgggcgatt atatccttaa acccgacttt aaatgagtat atcttatacg gtctaccctt
     4921 gccagctttc ttttcttcac tctcatttat ccagtcacgt tcttttaatt gcttcataac
     4981 tatgcttact tcgggttgac gtaatctggc tgaccgttca agttctatcg atgtagctga
     5041 attcgtactt tgcatatatg caagtgtcat tgcgacattc ttgctcattc ccaaaataat
     5101 aagtgcatct gcaatttctt cgtctttctt gtcaagttgt ttcattttaa tggttttcat
     5161 aatgatttat atattatatt aggcattata ttaatcttcc gatttatgct tacataatca
     5221 gaaaagttct tcatacgtgt tcggttaagg ggaagtccca gaacataata tcacataata
     5281 tacatagttt ttatgtattt tatatatttc ggttagaaaa ggtaaatatt tatgagttga
     5341 aaacatcatc tataggtggc gtctgagatc taaatgtccc aaaacatttt atcacactca
     5401 gacgcttatt taattagttg aaaaaggaag atttcgagaa aaaaaaactt atttaaagtc
     5461 accaacacta attgcaacgg tcacccagga cggtaggaac caccaattgg tgcacaccag
     5521 ccacctgaaa taagaggaag tgtttatgtt gtcagatctc ttgaggctgc actctgggct
     5581 ttctatagta gtgattcatt caaagacagt ttgcaggagc ttattatagc gataatggta
     5641 ttccagtgtt gtggcgctca aaactggatc aacgaaattt gatcgactta tatgctgaat
     5701 ggctttttga acacaggaaa agtggctgcg cttgcgaacc ggatataatc taagagggtt
     5761 acaattatat ataattttca agctgcagta tgtcatttaa cgacttctat tgtaatactg
     5821 ttgtgattat gattttactt ccaaaaatat ttaagctttt tcataggtga catggtacgg
     5881 ttttagaaat ctctttcata aaaatatctt aggttcacat acaacaagat cacgtcacat
     5941 catatataca gttcggtaca gagtcgtata attaaacgac gaaccaccat acatccatct
     6001 ccatttttgg cgttgcttgg atttcattca tta
//
LOCUS       DATGBN010000159         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_554989, whole genome shotgun sequence.
ACCESSION   DATGBN010000159 DATGBN010000000
VERSION     DATGBN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554989"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            773..2386
                     /locus_tag="VKL21_03695"
     CDS             773..2386
                     /locus_tag="VKL21_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMB44906.1"
                     /translation="MNKKHALASMNKLKPVLIALLIATVFTGITVVASSAENKNTVMI
                     NFQVDAENTGEHLFKVLGELEGRGYTTTVYVTGSFAENNGHIIQEIQDHGHDIAFHGW
                     ATGENLTTMNYSMQQKILINAKNSVENYSGQVEGFRPQYYSQNEDTYTILDSLNISYD
                     SGFISGLKYIPGYENYSIPYKVPNHSFYAVPVSSYRTPDKIVYLCDLSASIKFKLNAT
                     EWSSILNKKFDVSKKNNEPMVVVIHPWITGNETTGYWQAFTAFLDRIENKNIDIVKTS
                     ELLEFYIKKDDRHAFILEGFPSKITLLELYVEKDDHHAKLYSDVSKCSVPTKIVLVRH
                     GETSWNALGILQGNANVPLNENGSAQAQKLADSTYSKTVNAVYSSPLSRAHDTAQAIA
                     DVHQLPVKVRGNLREIGVGIYTGFKASQIPSEIRISWSTNPYFAMPSGFANTTNLLDP
                     SYVEGIYFEGESLDMAADRSWHSITNLAKQHCGENVVTVTHGGIIQMALTQVKGLNIT
                     EYKNISVPTASQTVLEFEPNDSVVLLPNW"
     gene            2760..3080
                     /locus_tag="VKL21_03700"
     CDS             2760..3080
                     /locus_tag="VKL21_03700"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HMB44907.1"
                     /translation="MVEQLGDLLALSVPIAGIVLTLCLTALAIYLDYKKKMAMIEKGL
                     VPEDEKPKPANRLGWGIAILGIGISFVAARLFNLDAITTGGLILTSIGMALLVSNKIS
                     KQQQ"
BASE COUNT          998 a          572 c          649 g          961 t
ORIGIN      
        1 cagtcgtgtt acaatggcag tctcctgtaa tgaggtggtt ttcattgcca gttccagcac
       61 gaaagaagga gttttgcttt tcatagttca aattgttgta tggatagttt tggtcttaag
      121 tcttggctga gcgatgcgaa agtattccgt attattgcaa aggggaaatt catctccacg
      181 actgaaggcg ggagctttct cccccctttg aacccatttc tgtaaattct ctttcatttt
      241 tttaaaataa tattgaaaat cttttattcc tggagaagtc gcccagcttt caatgtttca
      301 tcttcattga caacgaggtg aatttgtact tttaaaaaca gaaaaagaac taaaaaagca
      361 ttcagagaaa tttaaatgtt tttcaagatg tcaatagagg aagatgctgg attgttatta
      421 gatatagtgg agaaaatgat ccggtttttc tttttaatct ccgattcgat cctcaacact
      481 tcccatggct taagaagcct gtaaaattct gtttttttaa cttcagggac acaacaaaac
      541 ggctggtttt ttccaatatt caatcctcaa agttcttttt gaatatgtga tgtattatat
      601 cctatttgta aaagttaacg acaactaaag atatttgtat ttaatgagca tgtaaaacta
      661 tatatgtaag aaatttgtgt tttcattgag ataaatggtg gtttagaaaa tgagattcca
      721 gtcttgagac cacattctaa agatgaggga ttatggaaaa ggagatgaaa aaatgaataa
      781 aaaacatgca ttggcttcta tgaacaaatt aaaacctgtt ctcatagctc tattaattgc
      841 aactgtattt acaggaatta ccgttgttgc cagttctgct gaaaataaga atactgtaat
      901 gatcaacttt caggttgatg cagagaatac gggtgaacat ttgttcaaag ttttaggaga
      961 gcttgaagga agaggatata cgacaaccgt atatgtaaca ggttcttttg cagaaaataa
     1021 cgggcatata attcaagaaa ttcaagacca tggtcatgat atagcatttc acggctgggc
     1081 aacgggggaa aacctcacaa caatgaacta tagtatgcaa cagaaaatat taatcaatgc
     1141 aaaaaactca gtggaaaatt actccgggca ggttgaaggt tttagaccgc aatattacag
     1201 ccaaaatgaa gatacgtata ctattttaga ttctttaaac atatcctatg atagtggttt
     1261 tatttctgga ttgaaatata ttcctggata tgagaattat tctatacctt ataaggtgcc
     1321 gaaccacagc ttctatgcag ttccagttag ttcgtacagg acacctgata aaatcgtcta
     1381 cctatgtgac ctctcagcat caattaaatt caagttaaat gcaactgaat ggtcttctat
     1441 attaaataaa aaatttgatg taagtaaaaa aaataatgag cctatggttg tggtaataca
     1501 tccatggatc acaggaaatg aaaccactgg gtactggcag gccttcactg cgttcttaga
     1561 tcgcatagaa aataaaaaca tagatattgt caaaaccagt gagctcctag agttctatat
     1621 caagaaggat gatcgtcacg ctttcattct tgaaggattc ccatctaaaa taacactcct
     1681 ggagttgtat gtcgagaagg atgaccatca cgctaaactc tattctgatg tttcgaaatg
     1741 ttccgtacca acgaagatag ttcttgttcg tcatggtgaa acttcttgga atgcattggg
     1801 catcttgcaa ggcaatgcaa atgttcctct caatgagaat ggtagtgcac aagcgcagaa
     1861 gttagcggat agtacctata gcaagacagt taatgctgtc tactctagcc cactttctcg
     1921 tgctcatgat acggctcaag ctattgcgga tgtgcatcag cttcctgtaa aagtgcgtgg
     1981 aaatctcaga gaaattggag tcggcatata cacaggattt aaagcaagcc aaattccttc
     2041 agaaattcgg atatcatggt ctacgaaccc ttatttcgcc atgccttctg gctttgcgaa
     2101 tacaactaac ctgttggatc cttcttacgt cgagggcatc tacttcgagg gcgagtcctt
     2161 agacatggcg gcagaccgct catggcatag cattactaat cttgccaaac agcattgcgg
     2221 tgaaaacgtc gtgaccgtta ctcatggggg tatcattcag atggctttaa ctcaagtcaa
     2281 aggccttaat ataacggaat acaagaatat ctcggttcca acggcctctc agactgtgtt
     2341 ggagttcgag cctaacgact cagtcgtttt gctgccgaat tggtaaatcg tctgtaaaat
     2401 gcttcaatta tttatggtgc gaaactcggt aagccctata cactcccaat atcaggggta
     2461 ggagccccgt aaggagtcac gaaggtgcag agggtaaagg atatgataga aagcgaaggt
     2521 cggcctggta gcgcgtttgc aattcatggg atagctttct tgtcgcacta ataacactta
     2581 tatgcagttc tgacaaggac tttaccagaa aacaatatcc gtagacgatt tagccatttt
     2641 catttatatc tcatataatc gcttccatta ttgcgatatg agcgattgtt ttgtgaccag
     2701 gtggacacat tgtcagaagc tggtaagttt tattaactga taaaacaata agcaccccca
     2761 tggttgagca gttaggtgat ttacttgcat taagtgttcc tattgctggt atagttctta
     2821 ctttatgtct gacggcattg gcaatttatc ttgattataa aaagaaaatg gctatgatag
     2881 aaaaagggct tgtgccagaa gatgaaaaac ccaaaccagc aaatagactt gggtggggaa
     2941 tagctattct aggaatcggc atatcgtttg tagctgccag gttatttaat ctggatgcta
     3001 ttaccacagg cgggcttatc cttacttcta ttggtatggc attattggtt tcaaataaga
     3061 tttcaaagca acaacaataa atcctaaaaa ggtaggaccg ctcatagatt ggctcattca
     3121 tcgacaagta tcacaaattc aatttatcgc cggatggatg cagaaatagc tctatttata
//
LOCUS       DATGBN010000160        12741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_516663, whole genome shotgun sequence.
ACCESSION   DATGBN010000160 DATGBN010000000
VERSION     DATGBN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516663"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VKL21_03705"
     CDS             <1..400
                     /locus_tag="VKL21_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44908.1"
                     /translation="SIHAYPDTATPEIKHISKVVEDAKKRYKGQDAFIVLGDLNADCT
                     YFKNNRQSPLRSGDYYWVINKSLDTTTTKRTDCAYDRIIITTPALTDFTGESGVLRFD
                     TAYNLSYDSTIAVSDHYPVYANFWSNNDTD"
     gene            complement(423..1094)
                     /gene="tpiA"
                     /locus_tag="VKL21_03710"
     CDS             complement(423..1094)
                     /gene="tpiA"
                     /locus_tag="VKL21_03710"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMB44909.1"
                     /translation="MGKKPLIVLNFKTYSESMGEKAVLMAKYCQEVSIESGIDIISCP
                     QTPDIFRVASAVKIPVFAQHIDSVGAGSYTGHITADCVRSNGAGGTLINHSERRLLLS
                     EIDSAIQSARKAGLRTIVCTNNIAVTSAAAALFPDFVAVEPPELIGSGIPVSKANPEI
                     VSGSVGAVKKINPRVRVLCGAGISTGDDVASAIELGTVGVLLASGVIKAKDPRAALFD
                     LVKKI"
     gene            complement(1113..1883)
                     /locus_tag="VKL21_03715"
     CDS             complement(1113..1883)
                     /locus_tag="VKL21_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB44910.1"
                     /translation="MVDKKKTKIEKGDSKRVPTGIEGFDELCGGGLLKNRSYLLSGAA
                     GCGKTIFGLQYLYNGITKYGENGIYIATEERPDQVRENVWKFGWDFEALEDEGKLAII
                     DACSTKIGIPSHEKYVDVRPFDLRSMMDQIIAIQDTIDAKRCLVDGTTSIGFYLQDPS
                     KIRVELLKLSTTLEVLGLTSLMTCEIIDDNSTSRFGVETFVTEGTILMYNKRVDSVRI
                     RSIEVFKMRGSDHSKNIHPYEITPQGIVVHPGEEVYTP"
     gene            2041..4743
                     /locus_tag="VKL21_03720"
     CDS             2041..4743
                     /locus_tag="VKL21_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HMB44911.1"
                     /translation="MGSLNLDSIFNPKSIVLIGASDEEGSVGYILMKNLTEMDYKGLV
                     YPVNIHKQEISGKKAYTAVSQLPQTVDLAIIATPAATVPSLVEECGNAGIKGIIIISA
                     GFKEVGASGKILEEKILEIKKKYNMRILGPNCLGIMHPDIGLNATFISKKQEIGNIAF
                     LSQSGALGSAILDLAAHENIKFSNFVSVGSMIDVDFGDLIDYFGTDPKTRSILMYIEG
                     ITDARKFMSAARHFSRTKPIIVVKSGRSTESAKAAASHTGAITGEDMVYDAAFKRAGI
                     VRVKDIADLFNCAEAIGKHPLPRGPNIAIITNAGGPGVMASDAVITLGGKLAQLSNAT
                     IEKLNSILPPHWSKSNPIDLLGDAKAQRYEAAAEACFNDEKVDGLLIIYTPQGSSDPA
                     EIAEKILDLYKNKKFSKPYFASFMGYEEVEKANHILTDNGVPTYSTPEQAVATYMYMY
                     QYKRNLELLYETPVELPVDSIPPKQPLVSIIRNAVKEDRNILTETESKRFLEYYNIPI
                     VKTRVAKTVDEAVTCASQTGYPVALKILSPEISHKTDVGGVKLDINSETELRSAFEDI
                     IKQVKERKPDAHIQGITVQKMVKKKGIEIILGAKTDSLFGPVIMFGRGGVDVEIYKDV
                     AIGLLPLNQTLARRMMEETKVYQLLKGYRNLPPANIKLLEEIIVRFSRMMVDFPQLKE
                     FEINPLLINENEAFALDARAVINSERIFSRSHEHLVISPYPEKYKSLWKLRDGRTVIL
                     RPIKPEDEPMWLEMFHNFSEESIHFRLFQTIIEPVAHEFSARYCNIDYDRELAIVAEM
                     EDNGQRKMLGVVRLNIDPSEKTGEISFIVADQWQGQGLGSKMVDYVIEICKDKKLEKI
                     NAVMLPDNFRAIQLFREMGFSIESMDDGTQKAILNLDEELTIPS"
     gene            4756..6000
                     /locus_tag="VKL21_03725"
     CDS             4756..6000
                     /locus_tag="VKL21_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMB44912.1"
                     /translation="MKNMNLQKFSAIILVALLIGMVSVPASLADSNSEQTMIKSDGRN
                     ICSVSEKDYSSKTYEENIYLVKFCSDPAEYPPVGIHVLKKKEIKPKEFNILLPGTGFN
                     SSTNFFVNEKKSMAEFLADKGILVVGVDYPETNIEFDPTADYNYMATMGLSQHTDDLE
                     KIVKLVQKETGISKYSVVGHSLGGLIGLDFASKHSSDRNLLGLYVIDTAGTLNSSQEP
                     QLVQSQRDDYNATAALISDGKTVNFEILGLAILAVNAKTDPGGDSGVPNPTGVNWTNY
                     QYLLIASIYTGLIPGNEIFVQGFFAGDLFKGLYHTPIETVYSIASQGKIFPLKINEDM
                     FGIYAGVPGSYQINWKNIKVPVKWENFELGFGSRGEEAANLIKQGGNQNVRFWVVPGY
                     AHIDGVYSLKARQDVWNPLFAY"
     gene            complement(6120..7259)
                     /locus_tag="VKL21_03730"
     CDS             complement(6120..7259)
                     /locus_tag="VKL21_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44913.1"
                     /translation="MNTNFRFKILIVFFLFIIIFPSNTNASEFRSGDKLVITKDEVVN
                     DDIYFAGSSVIVEGTINGDLVAAGGEIRVTGTINGGIIAAGGSIIVNGKVTDDIRAAG
                     GEVRIGGDVGDNVLTFTGQFVLEKNARIARDLTLGAGNAVIDGTVNGNINGGATDLEM
                     SGSVKGNVTVEIDNNIKIFPDAIIGGNLEYKAPHQGEISGIVSGKTTYKETPVKKEDI
                     KSKIEGEILGYLWLLLIGIVSLLLAPELTQKNSDMVTVKPLRNLLFGLLFLIITPIVA
                     VLLLITIIGIPLSLIIFIIYVIILYISRIYVGFRAGQYVLKKLKIETRSRGLTMASGL
                     LILIIGMNLPIIGIFIHFAVLLLGLGAIALTEYDIYRKLREQKVI"
     gene            7411..10158
                     /locus_tag="VKL21_03735"
     CDS             7411..10158
                     /locus_tag="VKL21_03735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HMB44914.1"
                     /translation="MVNELGDIRIMLNPKTVALIGATEREGSIGRTVLENLLLSDKRK
                     IFPVNPGRKSILGIDCYPDITSIPEKIDLGIVVTPANTVPDVVESCGKAGVEGLIIIS
                     SGFKEFGEEGKELEERIKEIRKKYGLRIIGPNCLGVIIPAIGLNASFLKVNPQAGKIG
                     FISQSGALGTAILDWAVDNCIGFSMFASLGSMIDIDFGDLVDYLGDNRETRSIMLYME
                     GVGNAKKFMSASRGFARNKPIIIVKPGRFSESAKAALSHTGSMAGDDQVYEAAFKRAG
                     VIRVKEVADLFNAAEVLVSKNLPKGNHLAIITNAGGPGVLATDTLMELGGKLSVLSEK
                     SLSELNSFLPPYWSKSNPIDVLGDADIDRFTKSINVCLEDEEVNGILIIYTPQGKAEP
                     GELSRKIAEIGINSSKPIIIVWMGGRKVEEAKKISIQNGIPTYETPEEAVKTYFYMYR
                     YASNLKLLYETPADIPVDQAPPKSNLKALIRKAVKEGRTVLDEEESKRFLGNYGIPNT
                     KPIVARSVDEAVRIAVNMGYPVVLKIASPDITHKSDIRGVVIGISSEEKLRVEYEQLI
                     SRVKEKLPEARISGVAVQKMIEKVDYEILLGAKRDNDFGSVILFGRGGVDAEIIGDFS
                     IGIPPLNQTLARIMMEHTKVYKMLKGYRGRKPADMRQVEQIIINFSNLIVDFPEILEI
                     DINPIAISDGKAYALDSRIVVDKNSMEINTLYPHLVITPYPTKYVTPWKLSDGTDVIL
                     RPITPEDEPLEFEMLSSLSENSLRERFFRTVNKITHEMLIRFCNIDYDREMAIVAEMK
                     SPEEKKFIGILGAQILPQIQHFETPEGKRLIGIARVIMDSDYKKGEFAVVVHDDFQSK
                     GLGHKLIDMMIGVAQEKGLEKIYGMVLADNIKMIRTCEDMGFTVTNQSDGLSMVELPL
                     R"
     gene            complement(10159..10425)
                     /locus_tag="VKL21_03740"
     CDS             complement(10159..10425)
                     /locus_tag="VKL21_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44915.1"
                     /translation="MYDEDLNILENLKNCTFISVCDDLRNCKKCENKNLLKKFKTGRG
                     YYSFEEILHRGNRRLIKNLKEMPTLSKATKILLAEIEQKNSHLL"
     gene            complement(10510..11241)
                     /locus_tag="VKL21_03745"
     CDS             complement(10510..11241)
                     /locus_tag="VKL21_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44916.1"
                     /translation="MKQIQITLTPPESKRIIAMGVKKLTIIRNALENGRILITLGTTN
                     AYVAEELTGRKIDHSRYAAGYIGEKAEVVPNDRRLPSIALKNGKEVNADTILNEMTSD
                     DVVIKGANAIDPDGVAGVMMANPVGGTTGGFMGIVMAKGINLVIPVGLEKSIPFSVID
                     ISSRIGIQRCSRAMGLPVGMMPLVGEVITEVEALLLLGAEDAFAIGAGGVNGGEGSIT
                     LCVVGENVDEIFDLVQNVKDKKGEL"
     gene            11783..11956
                     /locus_tag="VKL21_03750"
     CDS             11783..11956
                     /locus_tag="VKL21_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44917.1"
                     /translation="MIFKLLFPNFTQTRAPLPLAAGVDLKAIDGVTVNIMELNVPGNR
                     EGTIYFDYLKGSY"
     gene            complement(12011..12295)
                     /locus_tag="VKL21_03755"
     CDS             complement(12011..12295)
                     /locus_tag="VKL21_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HMB44918.1"
                     /translation="MPLKEIRAKLEKIDAQIIRLIDQRTALAKDVLDAKKAEKKPIND
                     ADQNKVVLERVANMATERGLDGEEVKRIFEILIKMNIDRQHEMSGEGNLP"
     gene            complement(12286..>12741)
                     /locus_tag="VKL21_03760"
     CDS             complement(12286..>12741)
                     /locus_tag="VKL21_03760"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMB44919.1"
                     /translation="GAFDDACASMLGGFVITDNKKKELIKREERDWQVLILAPEKRVL
                     SSGTNVMRSRLIGKWVEMAYKEAVTGNYEKAMTLNGFLYCAALGFGTEPMMIALETGI
                     EGVSLSGTGPAYTALGSPELLDKLEPLWNRLGAKVIRTKVNNTGGKACR"
BASE COUNT         3821 a         2615 c         2604 g         3701 t
ORIGIN      
        1 atctatccat gcatacccgg atacagctac tcctgaaatt aaacacatct caaaggtggt
       61 cgaggatgct aaaaaaaggt ataagggtca ggatgctttc atagttttag gtgacttaaa
      121 cgctgattgc acttatttca aaaataacag acaatcaccg ctgcgaagtg gagattatta
      181 ttgggttatt aacaaaagcc ttgacactac taccacaaaa agaactgatt gcgcatacga
      241 caggatcata ataactaccc ctgcattaac tgactttaca ggggagtcag gcgtgttgag
      301 gtttgataca gcttataacc tgagttatga tagcacaata gcggtttctg accattatcc
      361 tgtttatgct aatttctgga gcaacaatga tactgattga tcgatttcat tcctgctact
      421 gttcatatct tcttcaccag gtcaaatagc gccgccctgg ggtcttttgc cttgataact
      481 cctgatgcaa gaagcacacc aactgttccc agttcaattg cggatgctac atcatctccc
      541 gtggaaatgc cggcgccaca aaggactctg accctggggt ttattttttt caccgcgccc
      601 accgaaccgc taacgatttc gggatttgct tttgaaacag gaatccccga accaataagt
      661 tcaggtggct caacagcgac aaaatcaggg aacaacgccg cagcagccga tgttactgca
      721 atattattcg tgcatacgat tgttcttaat cctgcttttc ttgcagactg aattgctgaa
      781 tcaatttcag ataacaggag acggcgctca gaatggttta taagtgttcc gcctgcccca
      841 tttgatcgga cacaatctgc tgtgatatga cctgtataac tccccgcgcc cacactgtcg
      901 atatgctggg cgaacacggg tatttttaca gcgctggcaa ccctgaaaat atcaggtgtt
      961 tgcgggcagg atataatatc gataccggat tcaatactga cctcctggca atatttcgcc
     1021 atcagcaccg ctttctcacc catgctttcc gaataggttt tgaaattcaa cactatcaaa
     1081 ggttttttac ccatggaatt caaaaaaaag aattaagggg tataaacttc ttcgcccgga
     1141 tgaacaacta taccctgtgg agtaatttca tatggatgga tgttcttgct gtggtccgaa
     1201 cctctcatct tgaaaacctc tatactccgg atcctcacgc tgtccacacg tttattatac
     1261 attaatattg taccttctgt tacaaaggtt tcaactccga acctgcttgt tgaattatca
     1321 tcaattattt cacaggtcat gagggaagtc aatcccaaaa cttcaagagt tgtgctcaat
     1381 tttaaaagct caactcttat cttggaagga tcctgcagat aaaaaccgat agatgtagtt
     1441 ccatcaacaa gacatctctt agcatcgatg gtatcctgta tcgcaattat ctggtccatc
     1501 atggagcgaa ggtcaaaagg tctcacatca acgtattttt catgagaagg tattcctatc
     1561 tttgtggaac acgcatcaat tattgcaagc tttccttcat cctcaagggc ttcaaaatcc
     1621 catccgaatt tccagacatt ttccctgacc tggtccgggc gctcctctgt tgctatgtaa
     1681 ataccatttt ctccatattt tgtgatgcca ttataaagat actgaagccc aaatatggtc
     1741 ttgccacaac ctgccgctcc tgacaacaga tatgatctgt tttttagtaa tccaccgcca
     1801 cacaattcgt caaaaccttc tattccggta ggaacacgct ttgagtcacc tttttctatc
     1861 tttgtctttt tcttatcaac catgaaattc aaccttgata taatattctt tcactatata
     1921 aatacttatt cgtcttatac ttataattat gagtcttcgg tcaatgtttt acttttatgc
     1981 aaatgatgca aagatttatt tgtccttatt cctattaatg atttaccggg gaggcgtatt
     2041 ttgggctcgt taaatcttga tagtattttc aatccgaaaa gtatcgtatt aataggcgca
     2101 agtgatgaag aaggctctgt tgggtatatc ttaatgaaaa acctcacaga aatggattat
     2161 aagggacttg tttatcctgt taacatccac aaacaggaaa tctcaggaaa aaaagcatat
     2221 actgctgttt ctcaacttcc tcaaactgtt gaccttgcaa taatcgccac tccggcagca
     2281 acagttccca gcctggtgga ggagtgcgga aatgccggaa tcaagggaat aatcattata
     2341 tccgccggat tcaaagaggt aggagcttcc ggaaagatcc tggaagaaaa aatccttgaa
     2401 ataaagaaaa aatataatat gaggatatta ggtccgaatt gcctgggaat aatgcatcct
     2461 gatatcggtc ttaatgcaac tttcatttca aaaaaacaag aaatagggaa tattgcattc
     2521 ttatcccaga gcggggctct ggggtcggca attcttgacc tggccgctca tgagaacatc
     2581 aaattcagta atttcgtgtc agtaggttcg atgatcgatg tggattttgg cgatctgata
     2641 gattattttg gcactgaccc caaaacaaga agtattctta tgtacatcga agggataact
     2701 gacgccagga aattcatgag tgcagcacgg cacttttcaa gaaccaaacc aataatcgta
     2761 gtgaaatcgg gaagatccac tgagagtgca aaggcagccg cttcacatac gggagctatt
     2821 acaggtgaag acatggtata tgatgccgca ttcaagcggg caggaattgt gcgggtaaag
     2881 gatattgccg atctattcaa ctgcgccgaa gccataggaa agcacccgct tcccagaggg
     2941 ccgaacattg caataattac aaatgcgggg ggccccggtg taatggcttc cgatgcggtc
     3001 ataacccttg gtggaaaact tgctcaatta agtaacgcaa cgatagagaa gctaaacagc
     3061 atcctgccgc cacactggag taagagcaac ccgatcgatc tacttgggga tgcaaaagcc
     3121 cagagatatg aagctgcggc agaagcatgc ttcaatgatg aaaaagttga tggcttattg
     3181 ataatctata ctccacaggg aagttcagat ccggctgaaa tagcagaaaa aattttagat
     3241 ctttacaaaa acaaaaagtt ttctaaacca tattttgctt cttttatggg ttatgaagaa
     3301 gtggaaaaag caaaccatat tcttacggat aatggcgtac cgacgtattc cacacctgaa
     3361 caggcagttg cgacatatat gtatatgtat caatataaac gcaaccttga gttgctttat
     3421 gaaaccccgg tagagttgcc agtggatagc attccgccaa aacagccatt ggtttccatt
     3481 attcgaaatg ctgtaaagga ggatcgaaac attctgactg aaaccgaatc aaaacgcttc
     3541 cttgaatatt ataatattcc tattgttaaa acaagagtgg caaaaaccgt agatgaagcc
     3601 gtaacttgcg cgtcccagac aggttatcct gtggcgctca agatattgtc gccggaaata
     3661 tctcataaaa cagatgtagg aggcgttaaa cttgatatca attcggaaac cgaacttcga
     3721 tcggcttttg aggatatcat aaaacaggta aaagagcgca aacctgatgc acatattcaa
     3781 gggatcactg tccagaagat ggtcaagaaa aaaggcattg aaataatcct tggcgccaag
     3841 actgattctc tttttggccc ggtaattatg ttcggaagag ggggagttga tgtggagata
     3901 tacaaagacg ttgctatcgg gcttttgccg ctaaaccaga cccttgcaag aagaatgatg
     3961 gaagaaacaa aagtctatca gcttctaaaa ggttacagaa atcttccgcc tgcaaatatc
     4021 aaacttctgg aagaaataat tgttcgcttt tccaggatga tggtggattt cccgcagtta
     4081 aaggaatttg agatcaatcc gctattaatt aatgaaaatg aggcttttgc ccttgatgca
     4141 cgcgcagtta ttaattccga acggatcttt tccagatccc atgaacatct tgtaattagc
     4201 ccctacccgg aaaaatataa gtccttatgg aagcttcgtg atgggcgaac agtgatcctg
     4261 aggccaataa aaccggaaga tgaacccatg tggcttgaga tgttccataa tttttcggaa
     4321 gaatccatac atttcaggtt atttcaaacc ataatagagc cagtggcaca cgaattcagt
     4381 gccaggtact gtaatatcga ctatgatagg gaacttgcaa tagtggcaga gatggaagat
     4441 aatgggcaac ggaaaatgct cggtgttgtc aggcttaaca tcgacccctc tgaaaaaacc
     4501 ggggagatat cttttattgt ggcagatcaa tggcagggcc agggtcttgg ttcaaaaatg
     4561 gtggattacg tgatcgagat atgcaaggat aagaaacttg agaaaatcaa tgcagttatg
     4621 ttaccggata atttccgggc aatacaacta tttcgggaaa tgggattttc tattgaatca
     4681 atggatgatg gcacacaaaa agcaattctt aacctggatg aggagttaac tatcccctca
     4741 taaatggtgg gaagtatgaa aaatatgaac cttcaaaaat tctctgcgat tatactagtc
     4801 gcattgctga taggcatggt ttcagttcca gcctcgttgg ctgattcgaa cagtgaacaa
     4861 acaatgataa agtcggatgg tagaaacatc tgttctgtat ctgaaaaaga ttatagcagt
     4921 aaaacatatg aagaaaatat ttatctggta aaattttgca gtgaccctgc agaatatccg
     4981 ccggttggaa ttcatgtttt gaaaaagaaa gaaataaaac cgaaagaatt taacattctt
     5041 ttgccgggaa ccggattcaa ttccagcacg aatttcttcg taaatgagaa aaaaagtatg
     5101 gcagaatttt tagcagataa aggcattctg gtagtcggtg tagattaccc tgaaactaat
     5161 atcgaatttg atcccacagc tgattataac tatatggcga caatgggttt atcgcaacat
     5221 acagatgatc ttgaaaaaat cgtaaagctt gtccagaaag aaacaggaat ttctaaatat
     5281 agcgtagtcg gtcacagttt aggcggttta ataggattag actttgcctc taaacattca
     5341 agcgacagaa atttactggg gctctatgtt atcgatacgg caggcacgct aaactcaagc
     5401 caagagccgc aattggttca atctcagcga gatgactata atgcgactgc tgctttgatc
     5461 agcgatggaa aaacagtaaa ttttgaaatt ttaggattgg ctatcctggc ggttaatgcc
     5521 aagactgatc ccggcggaga ttctggcgta ccaaatccaa cgggtgtcaa ttggacaaac
     5581 tatcaatatt tattaattgc ctcaatttat acaggtctga taccaggcaa cgagatattc
     5641 gtgcaaggat tttttgccgg agatctgttt aaaggtttat accatacgcc aatcgaaacg
     5701 gtttacagca ttgcctcgca aggtaaaatt tttcctttga agattaatga ggatatgttt
     5761 ggtatttacg caggagtgcc tggaagttat cagattaatt ggaagaacat taaagttccc
     5821 gtaaaatggg agaatttcga actcggattt gggtcaagag gagaagaggc cgcaaatctc
     5881 atcaagcagg gaggaaatca aaatgtccgt ttctgggttg tacctggtta tgcgcatata
     5941 gatggcgtgt attcgttgaa agccagacag gatgtttgga atccgctttt tgcatactaa
     6001 atagaattgg tgaaaggaaa tcttcaccat catgattatg aaccatgaaa aactctaaaa
     6061 ctgtcgtata agttatatga cattagtccg ataggttttc ttggagggat atgagaaaat
     6121 cagattactt tttgttctct taatttccgg tagatatcat actcagtcaa tgcgatcgct
     6181 ccgagtccaa gaagaagaac tgcaaaatga ataaatatcc cgataattgg caggttcatg
     6241 ccgataatca agatcagtaa acctgaagcc attgtcagtc ccctggacct tgtttctatt
     6301 ttcaacttct ttaatacata ctgtccagcc cggaatccaa cataaatcct gcttatatat
     6361 aaaataataa cataaataat gaatattatc agactcaagg gtatcccgat aatcgttatt
     6421 agaagcaata cggcaacaat gggtgtaatt atgaggaaca gaagcccaaa taataggttt
     6481 cttagaggct taaccgtaac catatcggaa tttttttgag ttaattcagg tgccagtagc
     6541 agagatacta taccgatcaa taaaagccag agatatccta aaatttcacc ctctatctta
     6601 gacttgatat cttccttttt taccggtgtt tccttgtagg tcgtcttccc tgatactatt
     6661 ccagagattt caccctggtg tggggcttta tattccaggt ttcctccaat tatcgcgtct
     6721 ggaaatatct tgatattatt atcgatttct acggtcacat ttcccttcac tgaaccactc
     6781 atttccagat ccgttgctcc tccattgatg tttccattca cagtgccatc aatgacagca
     6841 ttcccggcgc ccagggttaa atccctggct atcctcgcat ttttctccag aacgaattgt
     6901 cctgtaaagg tgaggacatt atctcccaca tccccgccaa tcctcacttc accgccggct
     6961 gcccttatat catctgtgac tttcccgtta acgattatgg aacctccagc cgcaatgatc
     7021 ccgccgttta tggttcctgt gacccttatc tcgcctccgg cagcaacaag gtctccattg
     7081 atcgtacctt ctacgataac tgaacttccc gcaaaataaa tatcatcatt taccacttca
     7141 tcttttgtga ttacgagttt gtctccggat ctgaattctg aagcattggt gttcgatgga
     7201 aaaataataa taaaaaggaa aaaaacgatc agtattttga agcgaaaatt tgtattcata
     7261 ccacccacta aggtatgtgt gtttgtcttt tcacataaat gttgcggcca gaagaactat
     7321 aatatatttt ataaataaat ataaatacat tcaaagaatt ataatcataa taagcttgta
     7381 tctcaataaa tataccttgt ttaagtcgaa gtggtgaatg aattgggcga tattagaata
     7441 atgttaaatc caaaaacagt tgcattgatc ggagctactg aaagagaagg ctccatcgga
     7501 agaacagttc tggaaaattt gcttttgtca gataagagga aaatatttcc tgtgaatcct
     7561 ggaagaaagt ccattcttgg cattgactgc tatccggata ttaccagtat tcctgaaaaa
     7621 atagatcttg gtatcgtggt gacgccggca aataccgtac ctgatgtagt tgaaagctgc
     7681 ggaaaagctg gagttgaagg attaatcatc atttcttcag gttttaagga attcggggaa
     7741 gagggaaaag aattggaaga aaggataaag gagatacgga aaaaatatgg attgcgtata
     7801 attgggccaa actgccttgg tgtcattata cctgctatcg gcttaaatgc ctctttttta
     7861 aaagtaaatc cacaagccgg aaaaattgga ttcatatcac aaagtggagc gcttggcact
     7921 gcaatacttg actgggcagt agataattgc attggattta gcatgttcgc atcactggga
     7981 tcaatgatcg atattgattt tggcgatctg gtggattatc tgggagataa tcgcgaaaca
     8041 agaagcatca tgctctatat ggaaggtgtg ggaaatgcca aaaaattcat gagcgcttca
     8101 agaggatttg ctcgcaacaa acctattatt atcgtaaaac ccgggaggtt ctcagaaagt
     8161 gcaaaagcgg cgctttcgca tacgggatca atggcaggag atgatcaggt ttatgaagcg
     8221 gccttcaaaa gagccggtgt tataagagtc aaagaagtgg ccgatctgtt caatgccgct
     8281 gaagttcttg tttctaagaa tctgcctaaa ggaaatcacc ttgcaattat tacaaatgcc
     8341 ggaggtccgg gagttcttgc taccgataca ttgatggaat taggcggaaa actttcagtt
     8401 ctctctgaaa aatctttatc tgaattgaat tcctttttgc cgccatactg gagcaaaagc
     8461 aatcccattg atgtgctggg tgatgcggat attgacagat ttacaaaatc catcaacgtt
     8521 tgtttagagg atgaagaggt aaatggcata ttgataattt atactcccca gggaaaagcc
     8581 gagccgggtg agctctcgcg taagatcgct gaaatcggga tcaattcatc aaaaccaata
     8641 atcatagtat ggatgggggg aagaaaagtc gaggaagcaa aaaagatttc catccagaat
     8701 ggcattccca catatgagac tcccgaagag gccgttaaaa catattttta tatgtacaga
     8761 tatgcctcga atctcaagct gttatatgag actccagcgg atatcccggt agaccaggct
     8821 ccgccaaaaa gcaacctcaa agcgcttatc aggaaggccg taaaagaagg acgaacagtt
     8881 cttgacgaag aagaatccaa gagattcctt ggaaattatg gtattcccaa taccaaacct
     8941 atagttgcaa ggagtgtaga tgaggccgtg agaattgcgg ttaatatggg ttatcccgta
     9001 gttctgaaga tcgcatcacc cgatataact cataaaagcg atatcagggg agttgtaatc
     9061 ggtatttctt ctgaagaaaa gcttcgtgtg gaatatgaac aattgatatc gagggtcaaa
     9121 gaaaagctcc cggaagcgag aatatcaggt gtcgccgttc agaagatgat cgaaaaggtc
     9181 gattacgaga tcttgcttgg cgcaaaacgc gataatgatt ttggctctgt gatactattt
     9241 ggcagagggg gtgtggatgc ggagatcata ggggatttct ctatagggat cccgcctctt
     9301 aaccagacac ttgcaaggat catgatggaa catacaaaag tttataaaat gctcaaggga
     9361 tacaggggga gaaaacctgc agatatgagg caggtcgaac agataattat taacttttca
     9421 aacctgatag tggattttcc tgaaatattg gaaattgata taaatcctat tgccatttca
     9481 gatggcaaag catatgccct tgattccagg atcgttgtag ataagaattc catggagatc
     9541 aatactcttt accctcatct tgtaattacg ccatatccta ccaaatatgt caccccatgg
     9601 aaattatcag atggtacgga tgtgatcctg cggcctatca ctcctgaaga tgaacctctt
     9661 gaatttgaaa tgctttcctc tctttctgaa aattcattaa gggagagatt cttccggaca
     9721 gtcaataaga tcactcacga gatgcttatc cggttctgta atattgatta tgatagggag
     9781 atggcgatcg ttgcagaaat gaaatccccg gaagagaaaa aatttatcgg cattctgggg
     9841 gctcaaatac ttccgcaaat acaacacttt gaaacccccg aagggaaaag attgatcggg
     9901 atcgcaagag tcataatgga ctctgattat aagaaagggg aattcgcagt cgttgtgcat
     9961 gatgattttc aatctaaggg cttaggacat aagcttatag atatgatgat cggggtcgcg
    10021 caagaaaaag gtcttgagaa aatctatgga atggttcttg ccgataacat taagatgatc
    10081 aggacatgcg aagatatggg atttacggtt acaaatcaat cagatggatt aagcatggtt
    10141 gagcttcccc tgaggtaatt atagaagatg tgaatttttc tgttcgattt ctgctaaaag
    10201 aatttttgta gctttgctta aagttggcat ttctttaaga tttttgatga gtcttctatt
    10261 tccacggtgc aaaatttctt caaaactata ataacctctt ccagttttaa attttttaag
    10321 taggttttta ttctcgcatt ttttacagtt cctgaggtca tcacagactg aaatgaatgt
    10381 gcagttttta aggttttcta aaatatttaa atcttcatca tacatgtttt cacttcacga
    10441 taagattcat ttaatacttt gaaatccatc atgttttctt gcttttttcg acatgattaa
    10501 ttttatttct tacagttctc cctttttatc ctttacattc tggacaaggt caaagatctc
    10561 atcgacattt tccccgacaa cgcacagggt gatggagcct tctcctccgt tcactccccc
    10621 cgcgccgatc gcaaaagcat cctctgcgcc caatagcagc aatgcttcta cctcagtaat
    10681 tacttccccg accagaggca tcatgccaac aggaagcccc attgccctgc tgcaccgctg
    10741 gatgccaatt ctacttgata tatctatcac ggaaaaaggt attgattttt caagccccac
    10801 cgggataaca agatttatgc cttttgccat aacgatcccc atgaatccgc ccgtagtgcc
    10861 gccaaccgga tttgccatca tcacgcctgc aacgccatcc gggtcaattg cattcgctcc
    10921 cttgataacg acatcatcgg atgtcatttc attcaggata gtatcggcat ttacttcttt
    10981 cccgttctta agggcaattg aaggaaggcg cctgtcatta gggacaactt ctgctttttc
    11041 cccgatgtat cctgctgcgt atctggaatg gtctattttc ctgcctgtta attcctcagc
    11101 cacataggca tttgttgtgc ccagcgttat caggatccgt ccattttcca gggcattccg
    11161 gataatggtg agttttttta cccccatagc tataattctt ttcgattccg gaggagttag
    11221 tgttatctgt atctgtttca tggattcatc acaaaacatt caatatacct gtgagtataa
    11281 aaatttgacc ggaatatagg aattaaaaaa aaactcgcag tattatgttt gtttattccg
    11341 gttactcaaa ttattatatt gttatatatc attttattcc ggtctgaccg cgaaaccatc
    11401 aaataaacgc acgccatcag acctattaaa gcagaaagta taagcaatac cgtggctgaa
    11461 tttttataga attcaattgc gatacttgtt cctattaaca tggctattgg cattaacaaa
    11521 cttaatatag taaaactttt gggaatcatt ttatcacctt tatcgaaata atttatcagt
    11581 gcaaaatttg cacattacta taattggatt ataaagtata taaatgtttc gcaacatttg
    11641 cacaataagc atgttttcgt aatcatgatt cgtcaaaaag tctttatatc ttcaatctaa
    11701 agattagatt ggcctttgca gaaaggggat accacccccc ttccatacca acattttttt
    11761 ttctgcaaag gcttcgaagg ttttgatatt taaattactt ttcccgaact ttacccaaac
    11821 ccgggctcct cttcctcttg cggcgggtgt tgacctgaaa gcgattgatg gcgttacagt
    11881 gaatataatg gaactgaatg ttccggggaa tcgggaaggt actatttatt ttgattacct
    11941 gaagggaagt tattgaaaaa ggggaggcac aaccacatat atcggagaga aatattatgt
    12001 tcctttcaat ctagggtaaa ttcccttccc cgctcatctc atgctggcga tcgatattca
    12061 tcttgatcaa aatctcaaat atcctcttta cttcttcccc atccaatccc ctttctgtgg
    12121 ccatgttcgc taccctttcc agaaccactt tattctgatc agcatcattg atgggttttt
    12181 tctctgcctt cttggcatca agcacatcct ttgccagagc cgtgcgctgg tcgataagtc
    12241 ttattatttg tgcatcgatt ttttctaatt ttgcccttat ctcttttaac ggcatgcttt
    12301 tccaccggta ttattgactt ttgtccttat cacttttgcg cccaaccgat tccataaagg
    12361 ttcaagctta tccagcagtt caggacttcc aagtgccgta taagcaggcc ctgtcccgga
    12421 aagacttacg ccctcgattc ccgtttcaag cgcgatcatc atgggctctg tgccaaaacc
    12481 cagggctgca cagtacagga agccgttcag ggtcatggct ttctcgtaat ttcctgtaac
    12541 tgcttccttg tatgccattt ccacccattt gcctataaga cgtgagcgca ttacatttgt
    12601 acccgaactt aacactcttt tttcgggcgc aagtatcagc acctgccaat ccctctcttc
    12661 tcttttgata agctctttct tcttgttatc ggttatcacg aatccgccca gcatagaagc
    12721 gcatgcatca tcaaaagccc c
//
LOCUS       DATGBN010000161         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_128581, whole genome shotgun sequence.
ACCESSION   DATGBN010000161 DATGBN010000000
VERSION     DATGBN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128581"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2385
                     /locus_tag="VKL21_03765"
     CDS             <1..2385
                     /locus_tag="VKL21_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMB44920.1"
                     /translation="LLIMVLASPYSLGISTLRDDAPKITVISDNTMSMDLFNKDLGKK
                     VFDALQPKTPTKFKEFAGIKSPIGDEVIGASENNNLVLVTDGNSNYGNKDLFDAISLV
                     SQTGTRVFAVEQKPLHNDISVEIASSKNIIIGNENVFNVVVRQAGNDSRYKLDVEIDG
                     KSVYSDSVTQNEREKSIPITGSFPTLGSHKITATITPATEDRFKLNNVFYRSVFVVPK
                     PKVLAVTSDTGSPLYTITTGLYDVTTAGSLPTDFSPYKAVIIDNKGATELNADILRNY
                     VGNGGGLMVVGGDMSYDKGQYNNSPIESMLPIISRAGEFKGGRNLIILIDSSGSTAFG
                     DAITGETFIGLIDANAISIVQNIGPENNVGVVAFGGLAATTDIMAMNSKANRAELEQF
                     IRQIGPKGGDNPTDLDVGLREAEKKLNSVSGTKAIIVISDGVMPPKGEGFEPIKNTVI
                     DLKNKGINISFTQVSTSYETNPIPFSSYSELAKVFDEQARVIDPSHKICNKDKCLESE
                     TPSEITPTPAPIKEIHEFPLVTIDTNHFITKYINITASVTGYNDVTPKLGSDWLLAAK
                     TPTGNKPILTTWGFGLGRVATFTTDNGNPSSDPTRPWASAVYSPGSSQLISSMINWAI
                     GDPRPKEGDVINAEDIWAGTAGKVIVTSDKQPPHVELDGAPLELARTGPTTYETSIDL
                     ETGDFHDLSGYGIAVNYPLEYREIGFNEKLKAVIESNGGRVYNEDEVEGLLFVDIKEK
                     AVRTVNEPKSEKEPYLLAALVLFLAEVIIRRLKDYRKERPVIEENQPRTAQVAE"
     gene            complement(2424..2897)
                     /locus_tag="VKL21_03770"
     CDS             complement(2424..2897)
                     /locus_tag="VKL21_03770"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB44921.1"
                     /translation="MHEKSLNLLKKKLIDLDFRNDWIPEFINHIEECIKKDAILILLF
                     GSRAKRDFHKDSDIDILIVSKHLSDDVRERASDFFSDSLPVQPFVMTPVELLERIDKL
                     DFLVFDAFEDGLILYSDMDLDSINGRLKASKERFSLKRIKNGWNFDAAEAEEAGL"
     gene            complement(2854..>3202)
                     /locus_tag="VKL21_03775"
     CDS             complement(2854..>3202)
                     /locus_tag="VKL21_03775"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMB44922.1"
                     /translation="DLCASEDSTSSKHYEWACFQAQQAAEKALKALIISKGSESRTHS
                     IKFLLSNLPENISVSDQIQNAARELDKHYIPTRYPDSFSTGIPKDFFTQDDAQRAISN
                     ARKIIEFVKEKIN"
BASE COUNT         1021 a          644 c          715 g          822 t
ORIGIN      
        1 ctcctgataa tggtcctggc ctcgccttat tccctgggta taagcacgct gcgtgatgat
       61 gccccaaaga taacagtaat ttcggataac actatgagca tggatctgtt caataaagac
      121 ctggggaaga aggtatttga tgcgttacag cctaaaaccc ccacgaaatt caaggaattc
      181 gcgggaataa aatcaccaat tggcgatgaa gtgataggtg catctgagaa taataatctg
      241 gtccttgtaa cagatgggaa ctccaattat ggaaacaaag accttttcga tgcaatttcg
      301 cttgtttccc agacaggaac aagggtgttc gccgtagaac agaaacctct ccataatgat
      361 ataagcgtgg agatagcaag ctcaaagaat ataataatag ggaatgagaa tgttttcaat
      421 gttgtcgtaa ggcaggcagg aaacgattca agatataagc ttgatgtaga aattgatgga
      481 aagtcagttt atagtgattc tgttacccag aatgaacgag aaaagtcaat tccaataact
      541 ggtagctttc caactcttgg ttcgcataaa attacggcaa cgataacacc agcaacagag
      601 gacaggttca agctaaacaa tgtattttac aggtcggtat ttgtggtgcc aaagccaaag
      661 gttctggcag taacgagtga tacaggctcg cctctttata ctatcacaac aggcctttat
      721 gatgtcacta ccgctggttc tctgccaact gacttttcac cctataaggc agtcataata
      781 gacaataagg gagcaactga gcttaatgcc gatattctgc ggaattatgt tggaaacggc
      841 ggcgggctta tggttgttgg aggcgacatg tcttatgaca aaggccaata taataattcg
      901 ccgattgaat cgatgctgcc cataatatca agagcgggag aatttaaagg aggaagaaac
      961 cttattatcc tgattgactc ttcaggtagc acggcttttg gagatgccat tactggagag
     1021 actttcatcg ggcttattga tgctaatgcc ataagtattg ttcaaaatat aggacccgaa
     1081 aacaatgtcg gtgttgtcgc tttcggaggg cttgcagcca caaccgatat aatggcaatg
     1141 aacagtaaag caaatagagc agaacttgaa cagttcataa ggcaaatcgg cccaaaaggc
     1201 ggggataatc cgacggacct tgatgtagga ctacgtgaag cagaaaaaaa attgaattca
     1261 gtgagtggca caaaagcgat tatcgtaata tcagatggag tgatgcctcc taaaggtgag
     1321 ggttttgaac ccataaaaaa tactgtaata gatttaaaaa acaaaggaat taatatcagt
     1381 ttcacgcaag taagtacatc atacgaaact aatccaatac cattttcatc gtatagtgaa
     1441 ctggcaaaag tttttgatga acaggcgaga gtcatagatc cttcacacaa aatatgcaac
     1501 aaagataaat gtctggaaag tgagacacca tcagaaataa cgcctacacc cgcgcccata
     1561 aaggaaattc atgaatttcc tctggtgaca attgatacga accatttcat cacaaaatac
     1621 atcaatatca cagcgtcagt aacaggttat aatgacgtca ctccaaaact cggttccgac
     1681 tggcttttgg cagccaaaac gcccacaggc aataagccta tccttacaac atggggtttc
     1741 gggcttggaa gggtggcaac gttcacgacg gataatggta atccatccag tgacccaacc
     1801 agaccctggg caagcgcggt ttacagccct gggagctcgc agcttatctc atcaatgata
     1861 aactgggcga tcggcgaccc tcgcccgaaa gaaggggatg ttataaacgc cgaggatatc
     1921 tgggccggaa cggctggaaa ggtcattgtc acatcagata agcagccgcc gcatgtggaa
     1981 cttgacggcg cgccacttga acttgcaaga acagggccga caacatacga aacctccatt
     2041 gatcttgaga ccggggattt ccatgacctc tcaggatacg ggatagctgt gaactatccc
     2101 ctcgaataca gggagatcgg ttttaatgaa aaattaaaag cggtcattga atcaaatggc
     2161 gggcgcgttt ataatgagga tgaagtcgaa ggattgctgt tcgtggatat caaggaaaag
     2221 gctgtgagga cggtaaatga accaaagagc gaaaaagagc cataccttct tgcggcactg
     2281 gttttattcc ttgctgaagt gataatcagg agactgaaag attacaggaa ggaacggcct
     2341 gtgatcgagg agaatcagcc aaggaccgcg caagtggcgg aatagaaaaa aaaataaccc
     2401 ggattgaagg aggatttttt tctctaaaga cctgcttctt cggcttcagc agcatcaaaa
     2461 ttccagccat ttttgatacg ttttaaagag aaacgttcct tgctcgcttt caaccgtcca
     2521 ttaatactat ccagatccat atctgaatat aatatcaagc catcttcgaa tgcatcgaat
     2581 acaagaaaat ccaatttatc tatcctctcc aatagttcaa caggggtcat aacgaatggc
     2641 tgcacaggca atgaatcgct gaaaaaatca gaagcccttt cgcgcacatc atcagacaga
     2701 tgtttggaaa caataaggat atcaatatcg ctatctttat gaaaatctcg ttttgctctt
     2761 gagccaaaaa gtagaataag aattgcatct tttttgatgc attcttcgat atggtttatg
     2821 aattcaggaa tccagtcatt tctgaaatcc agatcaatta attttttctt taacaaattc
     2881 aatgattttt cgtgcatttg atatcgctct ctgggcatca tcctgtgtga aaaagtcctt
     2941 tggtattcct gtagaaaaag agtcagggta tcttgtggga atataatgct tatcaagttc
     3001 tcgagcagca ttttgtatct gatcactgac tgaaatattc tccggcaaat tagaaagcaa
     3061 aaattttatg gaatgggtgc gggattcgct tccctttgaa ataataaggg ctttcaaggc
     3121 tttttctgct gcctgctgcg cctgaaaaca tgcccattca taatgttttg aactggtact
     3181 atcctctgag gcacagaggt ca
//
LOCUS       DATGBN010000162         4511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_283534, whole genome shotgun sequence.
ACCESSION   DATGBN010000162 DATGBN010000000
VERSION     DATGBN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283534"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..2300
                     /locus_tag="VKL21_03780"
     CDS             216..2300
                     /locus_tag="VKL21_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMB44923.1"
                     /translation="MITTLKSIPRIGEKTARRLVEHFGSEKQAIDAIMNRDIAAISEI
                     EGMTDKSAISLVLEAIAINDGAGVRDFLRTNEAFEFYDRLMELIKGFAHTDHARSKLS
                     LYFPYPSRKKEKIMQIQKDIRDIIEIADKLDEEELSSLLSRIKPLKTNNNIPVIRDRV
                     IIATNAQEFEIAKKFPVSVQLVSDVREIADVARGYSYLILDTHLAGLDFPEDIDVDFL
                     DIKKAQTWQIAPEKELAFFTKNLDSINDTIGIFNMIREHSPGFCEKIRQEDIERLSSG
                     LMRLSGEEDIKSGLDTEIDRCKSIIDRLSIAVTNSQVQANIEFGALIEKSSITVRGFD
                     LLTAMDGSINRLLEKEIREKYNTVTVNAIRGIVIELGLNPAESQYVNNFFNEEVSHPI
                     KVNIRGVEGLKNHLLRIIASRKIGLLRNNARSLSNLKETASGLVRDVLDFDIGYTIAC
                     FSKKFALNMPLLHSEMGIGIKGGTNLFLTNAVPIDYAVGSSSMNSGVEKLKDARVILL
                     SGVNSGGKTSTLDLLSQITILAHMGFPVPATECELGLVEEFYYFGKSKGTMDAGAFET
                     TIKDFSAVASKNTKIVLADEMEAITEPGASAKIISGLLEELDDNNGLGIFVSHLAESI
                     LKNTTHKIRVDGIEAKGLDEHLNLIVDRNPRYNYLARSTPELIVERLARKYEDIAFYG
                     KLLTKFRSSTSD"
     gene            2504..3007
                     /locus_tag="VKL21_03785"
     CDS             2504..3007
                     /locus_tag="VKL21_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44924.1"
                     /translation="MSDNRNESEFDLFRLFFRYLSRKLTFQHLLYFFVFVTFDIGDAV
                     TASMMMESRGISAEYNTFVQHIFVNYGLSGLIGAKLLMIIVPLIIASTAVKNSYWFIN
                     GVLVSLTLAGLMAIQANLQALASLPHMSPMEINLLYLKILFILAVAGMIMDSYFSDSA
                     EKGYSIK"
     gene            3057..3950
                     /locus_tag="VKL21_03790"
     CDS             3057..3950
                     /locus_tag="VKL21_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HMB44925.1"
                     /translation="MQLELSSQVDCLCDFTYDFYWQHQGTRLSPSAVIPRQIGTSYTY
                     QDLVLALKKGEDVNIKGNAGKRLGSSLGVDLKFFGGTGKSVPAGSIIVNGDVDTRMGI
                     SMVRGAIYVAGNVKQPIGNVVEVKSDRQGYRKFISITNMLDEGQIEKILHPNVMEDGC
                     MYLRDMIIRDTIAARLDADKKVIVEGDSGMSTGILMKRGFIHIEGDAGLNTAVLLRGG
                     TIITGNTGEFAGAYMKDGVLVIQGKARGYVGANIKGGTIFYKGEAMIPGFPVSEGDIR
                     MLVRLLDIGKMEAMMFKRFEI"
     gene            complement(3931..4227)
                     /locus_tag="VKL21_03795"
     CDS             complement(3931..4227)
                     /locus_tag="VKL21_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HMB44926.1"
                     /translation="MITHVVLFQLKDRSPESIEKARDILLGMKGKISQLRYLEVGTDV
                     LHSERSYDIALITKFDSMKELQAYQVHPVHVEVAKYIMSESQSAIAVDFISQIS"
     gene            complement(4243..>4511)
                     /locus_tag="VKL21_03800"
     CDS             complement(4243..>4511)
                     /locus_tag="VKL21_03800"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499514.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMB44927.1"
                     /translation="KIKGAFCVEGEIKDNPVLALFKYHITPRLPEIIINRPEKYGGNL
                     HYESYEVLESDFAAKTLHPMDLKVAAAQYMNEILEPVRKLMLEN"
BASE COUNT         1347 a          875 c         1003 g         1286 t
ORIGIN      
        1 ctcccaaagc tccttccgca ccgccaataa tgcccaccgc atacaatata taaccaactg
       61 ttttttcatt taattccata attccacctc atatacttta tatttgattt agagtataca
      121 ttatatttga ttgcaagtat attataaata gtttctcgtt aagtatttga gacctaacaa
      181 aacaactata gaatacaacc tgaaatggta ttccaatgat aaccactcta aaatccatcc
      241 cccgcatagg tgaaaaaacc gcgcgccgcc ttgttgagca tttcggatct gaaaaacagg
      301 caattgatgc cataatgaac cgggatatag cagccattag cgagatcgag ggaatgaccg
      361 acaaatccgc gatatccctt gtgcttgaag ctatcgcaat aaacgatggc gcaggtgtcc
      421 gggattttct aagaacaaat gaggcttttg agttttatga taggcttatg gaactcatca
      481 aagggtttgc ccatacggat catgccagga gcaaactgag cttatatttc ccttatcctt
      541 caagaaaaaa agaaaagata atgcagattc aaaaagatat ccgtgatatt atcgaaattg
      601 cggataagct cgatgaagaa gaactttctt ctctcttatc aagaataaaa cccctgaaga
      661 cgaataataa tattcctgtg atacgggata gggtcatcat tgccacaaat gcgcaagaat
      721 ttgagatcgc caaaaaattc ccggtatctg tccagcttgt ttccgatgtc agggaaatag
      781 cggatgtcgc ccggggttat tcgtatctga ttctggacac tcatcttgcc ggccttgatt
      841 ttccggagga tatagatgtt gattttcttg atataaaaaa agcccagaca tggcagatag
      901 cccctgaaaa agaacttgca ttttttacaa aaaaccttga ttccatcaat gacactatag
      961 ggatatttaa catgatccgc gaacattctc caggtttttg tgagaagata aggcaggagg
     1021 atatcgaacg tttgtcttct ggtttgatga gactttccgg tgaggaggat ataaaaagcg
     1081 gacttgatac tgaaatcgat aggtgcaaat ccattatcga taggctcagc attgcggtta
     1141 caaattcgca ggtgcaggca aatattgaat ttggggctct tatcgaaaag agttccataa
     1201 ccgtaagggg ttttgacctg ctcactgcaa tggatgggag tattaatcgg ctgcttgaaa
     1261 aggaaatcag ggagaaatac aatacagtaa cggttaatgc gattagagga attgttatag
     1321 aactgggcct gaatccggca gagtcgcagt atgtaaataa tttcttcaat gaggaagtat
     1381 cgcacccgat aaaagtaaac atacgcgggg ttgaaggatt aaaaaatcac ctgcttcgca
     1441 tcatcgcgtc aaggaagatc gggcttttaa gaaataacgc ccgttctctt tcaaacttaa
     1501 aggaaactgc ctccgggctt gttcgagatg tcctggattt tgatattgga tatacgatcg
     1561 cctgtttttc aaagaaattt gcccttaata tgccccttct ccatagtgag atggggatcg
     1621 gaataaaagg aggaaccaac ctttttttaa caaatgcagt cccgatagat tacgcggtgg
     1681 ggagttcaag catgaattcg ggcgtggaaa aactcaaaga tgcccgtgtc atcctgttga
     1741 gcggtgtgaa ttcaggggga aaaacatcaa cacttgatct gctctcccag atcaccatac
     1801 ttgcccatat gggttttccc gtccctgcaa ctgagtgcga actgggcctt gttgaagaat
     1861 tttattattt cggtaaatcc aaaggtacaa tggatgccgg ggcatttgaa actactataa
     1921 aggatttttc agcagttgca agtaaaaata ctaagatagt ccttgctgat gagatggaag
     1981 ccatcactga acctggcgct tccgcaaaga tcatttcagg gttacttgag gaactggacg
     2041 ataataatgg gctgggaata tttgtgagcc atcttgcaga atcaatacta aagaatacaa
     2101 ctcataaaat tcgcgttgac ggcattgaag caaagggtct tgatgaacac cttaacctga
     2161 tagttgacag gaatccccgg tataattatc tggcgcgaag tacgcctgag cttattgtgg
     2221 aaagacttgc gcgcaagtat gaggatattg ctttttatgg gaaattattg actaaattca
     2281 ggtcatcgac ttcggattag attagaatgt atttccttat ttcactttcc atggaattat
     2341 aataacattt ccccaggcat tccattttat tgaggagcat gaaaaatgct cattttaatt
     2401 cgataccggt tttatttggc ttgaaaataa tatagtatca atgataatta tcattatgat
     2461 aatgaaacca gtcgaagatt tcgttccttc aaggtgagat caaatgagtg ataaccggaa
     2521 cgaatcggaa tttgatttat ttagactttt cttcaggtat ctatcacgca aattgacttt
     2581 ccagcacctt ctttactttt ttgtttttgt gacttttgat attggagatg cggttacagc
     2641 ttccatgatg atggaatcaa gaggaataag cgctgaatat aatacatttg ttcagcacat
     2701 ttttgtgaat tatgggcttt caggtctcat aggtgccaaa ctattaatga tcatagtccc
     2761 cctcattatc gcttcaacgg ctgtaaaaaa tagctactgg ttcataaatg gcgtacttgt
     2821 ctctttgacc ttagctggct tgatggccat ccaggcaaat cttcaggcac tggcaagtct
     2881 tccgcacatg agtccaatgg aaataaacct cctgtatctt aagatcttat tcatacttgc
     2941 agtagccgga atgatcatgg acagctattt ttcagactcc gcagaaaaag gatattccat
     3001 aaaataacct gttccaaaaa aagaattaat ctactttgaa gtaaatctat ggagagatgc
     3061 aacttgaatt atccagccag gtagactgcc tgtgcgattt tacgtatgat ttttactggc
     3121 agcaccaggg cacaaggctt tccccgtccg ctgttattcc tcgccagatc ggcacctcct
     3181 atacatatca agaccttgtt cttgctttaa agaagggaga agatgtcaac ataaaaggca
     3241 atgcgggcaa aaggcttgga agcagcctgg gagtggattt gaaattcttt ggaggaacag
     3301 gtaaatccgt tcccgcagga tcgataatcg tgaatgggga tgttgataca cggatgggca
     3361 tcagtatggt cagaggagcg atctatgttg caggtaatgt taaacaacca attggaaatg
     3421 ttgtggaagt taaatctgat cgccagggtt atcgaaaatt catatctatt acaaatatgt
     3481 tagatgaagg acagattgaa aaaatccttc atcccaatgt tatggaagat ggctgcatgt
     3541 acttaagaga catgatcatc cgtgatacga ttgctgcccg ccttgatgct gataagaagg
     3601 tcattgtaga aggcgattcg gggatgagca cagggatcct gatgaagcgc ggatttatcc
     3661 atatagaagg ggatgccggc ttgaacacag ccgtacttct gcgcggcggg acgatcatca
     3721 cggggaatac gggagagttc gctggcgcgt atatgaagga tggggtcctt gtcatacagg
     3781 ggaaagccag aggttatgtg ggtgcaaata taaaaggtgg gacgatcttt tataaagggg
     3841 aggctatgat tcccggtttt ccagtgagtg aaggggatat caggatgctt gtcaggctac
     3901 tcgatatagg taagatggaa gctatgatgt ttaagagatt tgagatatga aatccacagc
     3961 aattgcagat tgcgattcgg acattatata ttttgccact tccacatgga cgggatgaac
     4021 ttgataggcc tgcagttcct tcattgaatc aaacttcgtt atcagggcga tatcatacga
     4081 acgttcggag tgaaggacat cggttccgac ttccaggtag cgaagttgtg agatcttgcc
     4141 tttcatacca agcaggatat ctctggcttt ttcaatgctc tcaggacttc tgtctttcaa
     4201 ttgaaaaaga actacatgtg tgatcataag actccaattt aactaattct ccaacatcag
     4261 tttccttaca ggttcaagta tctcattcat gtactgtgct gccgcaactt tcaagtccat
     4321 ggggtgcagc gtcttggccg caaaatcaga ctcaagcacc tcgtaactct cataatgcag
     4381 gttaccgccg tatttttcag ggcgatttat gataatttca ggcaaacgcg gcgtgatatg
     4441 atacttaaaa agggcaagta ccggattatc ctttatttca ccctcaacgc agaacgcgcc
     4501 ttttattttc t
//
LOCUS       DATGBN010000163         7226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_167843, whole genome shotgun sequence.
ACCESSION   DATGBN010000163 DATGBN010000000
VERSION     DATGBN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167843"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VKL21_03805"
     CDS             <1..247
                     /locus_tag="VKL21_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44928.1"
                     /translation="ATRIELKNKLAALLNSKPELIVIEHLDTIYGKQEMNATACLYQT
                     EEHLKQLAHKHLIARDAPRVVEGESAEAPAEPKPEAK"
     gene            263..412
                     /locus_tag="VKL21_03810"
     CDS             263..412
                     /locus_tag="VKL21_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HMB44929.1"
                     /translation="MAINKFYEISANKAKKIKPSCPRCGPGVFLGEHKNRTSCGKCGY
                     TEFKK"
     gene            505..804
                     /locus_tag="VKL21_03815"
     CDS             505..804
                     /locus_tag="VKL21_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44930.1"
                     /translation="MLTRRVQHELELLKRHLIILKKVIDSGHIGILKLSLETGIPDHL
                     VRYSLRVLEQQGLITPSTQGAVATKSAKEAYSEFKAELERIGEMTEDIKRIGFGD"
     gene            794..1252
                     /locus_tag="VKL21_03820"
     CDS             794..1252
                     /locus_tag="VKL21_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HMB44931.1"
                     /translation="METDGGGKKQKKYEYLYHIADAKFRAFGLTLEESFENAAIALFN
                     VMIDTSNMKGIQERKIELTSPDIKMLLVEWLSELLYLFEVDEIIFSEFRIDKIEKKGE
                     DFSLKGKASGVPIDLLYHKFDTQVKAVTFHELEVEQDNKGGFWVQVVLDT"
     gene            1311..2777
                     /locus_tag="VKL21_03825"
     CDS             1311..2777
                     /locus_tag="VKL21_03825"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HMB44932.1"
                     /translation="MADGSTKDVASILTKVNDYTWDIPMDYKPGMRVPGRIFVSSKLL
                     ESLEHDTLTQVSNVATLPGIQKFAMAMPDAHSGYGFPIGGVAAFDRDTGIISPGGVGY
                     DINCGVRIIKTNLTVDEVRPGMKELIELLFHEIPSGVGSKSKLRASDSELTDAFLHGA
                     KWAVESGYGVKEDVEHCEENGYMKIADPSKVGEKARKRGKPQFGTLGSGNHFLEVQYV
                     DEVYDPEVARVFGLHKGQVTVMIHCGSRGAGHQICDDYLKILDKATHKYGIQLPDRQL
                     ACAPAESTEGQNYYKAMAAGANYAWANRQVITHWVREAFTRFFRRDMDDLGMDLIYDV
                     AHNVAKLEEHTIDSGKKMVYVHRKGATRAFPPGHRDVPLIYRKVGQPVLIPGSMGTPS
                     FVLHGTQKAMELTFGSACHGSGRVSSRGAAKREFHGETIQKELAARGISVRATHPSII
                     AEEAPGVYKPSEEVVDVVHKLGIARKVVKFMPLGVAKG"
     gene            complement(2789..3085)
                     /locus_tag="VKL21_03830"
     CDS             complement(2789..3085)
                     /locus_tag="VKL21_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44933.1"
                     /translation="MKDENEPIPQESDACEIKTPDDHRMMFRALENPMRRNIVKSIGA
                     FGKTKNEIMKELKISESQLKFQLDFLIKECYAQVDGDKCRLNSKGLQELLANIK"
     gene            3208..3741
                     /locus_tag="VKL21_03835"
     CDS             3208..3741
                     /locus_tag="VKL21_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HMB44934.1"
                     /translation="MDTSLLHKENAPGIYKCAILTISTSRFEKYGKTDRPERAEDVSG
                     KLIWGMVQVKGSTIVNYELIPDNMEIIREAFKKDLYSEAEVIISSGGTGLSPNDVTIE
                     AVTPFIEKEMPGFGELFRLQSINQIGNSVMLTRAIAGVARGKIIFCLPGSPNAVKLAL
                     ELILPEMGHILKHLKGQ"
     gene            3742..5103
                     /locus_tag="VKL21_03840"
     CDS             3742..5103
                     /locus_tag="VKL21_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMB44935.1"
                     /translation="MEYKWKAMGVVWIGIFMATLDGSIVNIALPTLTKFFKTDVTTIE
                     WVVMAYLLTITSLLLSLGRISDMVGRKNIFAGGLVLFTIGSWLCASSTTEWQLIFFRV
                     FQGFGAAMVMATGVAIITHSFPPRERGKAMGLIGTVVSIGSLAGPVMGGFLIQNVGWQ
                     SIFYINIPVGIFGTVVALKVLQKEETINKDQTFDIPGGVTLFISLISLLLALSEGQEK
                     GWNSSFIISLFVSSIVFFILFVRIETTAKQPVLDLKHFRNRQFASANISALISFMAMF
                     SVILLMPFFLQDQLGYSTEKMGFVFMSVPLVMSVVSPLSGWLSDRTNSHVLSSIGIGV
                     AALSILSLGYLSLDSSFPDVALRLCFLGLGMGLFQAPNNSIIMGSLPKEQLGIAAGVM
                     GTMRNMGMVIGVAVSGAVFSNRFVFYGNEFLPAFRDTYIVSAIICGIAVLISLVRSRS
                     KIE"
     gene            5622..6653
                     /locus_tag="VKL21_03845"
     CDS             5622..6653
                     /locus_tag="VKL21_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44936.1"
                     /translation="MSVRKNISLEKGHLRKLEPLVLKHKGNFSAAMREIIDLIDTMTR
                     DPEAINNLIDGLKTDYNLTENVIFWLIKQAHGRLIDPEHLNSIIDPSKIVLLSDLEKY
                     MDEMTNGASWQTNIKIEDYDDNVNPSHATVILSGNSNYKMEFLGGLISSFLVLHKKQE
                     IISLKKMSNKIIVNFRKQMNANLARQSLINYFGDMEDLSSEINKKLDFWKCIVNLYKQ
                     TNYNMVTIPKNYYNELLIGKEAPSYLTAPIEAIHKIPIRDIPLDVLIPTMKSVYEYMG
                     IVERIDVNENTLYIYHGNTDKRAIRAIEKILLNVLNSNGIIFESRCSENLIVMSPVVT
                     KAEMVTITK"
     gene            6721..7062
                     /locus_tag="VKL21_03850"
     CDS             6721..7062
                     /locus_tag="VKL21_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44937.1"
                     /translation="MANMSEDALFKALLKNKPPQQPPKPAVVAQSVPRKEVPRPAAAP
                     PVERYQVEEPVIEPKVIPVVKPMNLEPVVDSINRLNSSISMIHGMMKTIVTPILILIL
                     IVLIGILIQTK"
BASE COUNT         2265 a         1362 c         1616 g         1983 t
ORIGIN      
        1 agctaccaga atagaattga agaacaaact tgcagctctg cttaattcca aaccggaatt
       61 gatcgtgata gagcaccttg atacgatata tgggaagcag gaaatgaacg caacagcgtg
      121 tctctatcag acagaagaac acctgaaaca acttgcccac aagcatctta ttgcaaggga
      181 tgcccccagg gtagtggaag gagaatctgc ggaagcgccg gctgaaccta aaccagaggc
      241 aaaataaagg aaaaggattt caatggcaat aaataaattc tacgaaatta gcgcaaataa
      301 agccaaaaag attaaaccct catgccccag atgcggaccg ggtgtatttc tgggtgaaca
      361 taagaatcgc acttcatgcg gaaagtgcgg ttataccgaa ttcaagaaat aatctttttt
      421 tcattttttt gattttatct cgcattgtat tggagataca tgctaaatta gatatgctca
      481 tagttacaag tctagatact gattatgtta acaagaagag tccagcacga actggaattg
      541 ttaaaaaggc acctcattat cctgaagaaa gtaatagata gcggacatat cggaatattg
      601 aaattatccc ttgaaacagg catacctgac caccttgtaa gatattccct tcgagttctt
      661 gaacagcagg gtttgatcac cccatctacg cagggcgctg ttgccacaaa atcggctaaa
      721 gaagcatatt ctgagttcaa ggcggagctg gaaagaatcg gggagatgac cgaagatatc
      781 aagaggatcg gatttggaga ctgatggcgg tggaaaaaaa cagaaaaaat atgaatatct
      841 gtaccatata gctgatgcca agttcagggc atttggtctt acacttgaag aatcttttga
      901 gaatgctgcc attgccctgt tcaatgtgat gatagataca tcaaacatga aaggtatcca
      961 ggaaaggaag atcgaattaa catcacccga tataaaaatg cttcttgtag agtggctttc
     1021 cgaattgcta tatttgtttg aagtggatga aatcatattt tctgaattca ggatcgataa
     1081 gatcgagaaa aaaggggagg atttttcatt gaaagggaaa gcatcgggcg tcccaattga
     1141 cctcctgtat cataagttcg atacacaggt taaagcagtg acttttcatg agcttgaagt
     1201 agagcaggat aataaagggg gattttgggt tcaggttgtt ttagatacat gaattgcgcc
     1261 agacttaata gactggagag catataatta acaagaaaag gaagaaaaac atggctgacg
     1321 gaagtacaaa agatgtggca agcatcctta ccaaagtaaa tgattacacc tgggatatac
     1381 ccatggatta taaacctgga atgagggtgc cagggcgcat ttttgtttca tcgaaattgc
     1441 tggaaagcct tgagcatgac actcttaccc aggtttccaa tgttgcaacc cttccgggaa
     1501 tccagaaatt cgccatggca atgcctgatg cccattcagg ttatggattc cccatcggag
     1561 gagttgccgc ttttgacagg gatacaggca taataagccc cggaggagtg ggatacgata
     1621 tcaattgcgg agtaaggatt atcaagacaa atctcacagt tgatgaagtg cgtcccggca
     1681 tgaaagaact catagagctt cttttccatg agatcccatc cggcgtgggc tcaaaaagca
     1741 agcttcgcgc ttcggacagt gaactgacag acgccttcct gcacggtgca aaatgggcag
     1801 tggaaagcgg atatggcgtg aaagaagatg tggagcattg tgaggagaat ggttacatga
     1861 aaatagccga tccctcaaaa gtgggtgaga aagcccgcaa acgcggcaaa ccgcaattcg
     1921 gtacgcttgg aagcggaaac catttcctgg aagtgcaata tgtggatgaa gtatatgatc
     1981 ctgaagttgc cagggttttc gggcttcata aaggccaggt aacggtcatg atccactgcg
     2041 gttcgcgggg tgcaggtcac cagatatgcg atgattatct caagatcctt gataaagcca
     2101 cacataaata tggcatccag cttccggacc ggcagcttgc ctgtgcgcct gccgaatcga
     2161 cggagggcca gaattattat aaagccatgg ctgcgggcgc taattacgca tgggccaaca
     2221 ggcaagtcat tactcattgg gtccgggaag catttacacg ttttttcagg cgggatatgg
     2281 atgatcttgg catggatctc atttacgatg tggcgcataa cgtggcaaaa ctggaagagc
     2341 ataccatcga cagtggaaaa aaaatggttt atgtccatcg caagggagct acacgggcat
     2401 ttcctcccgg acaccgggat gttcctctta tttacaggaa ggttgggcag cctgtcttaa
     2461 ttcccgggag tatgggcacc ccatcttttg tgctgcatgg tacccaaaaa gcgatggaac
     2521 ttacattcgg gagcgcatgc catggatccg gcagggtctc aagccgcggg gcggcgaaac
     2581 gtgaattcca tggtgagaca atacaaaaag agctggcagc aaggggaata tccgtcaggg
     2641 ccacgcatcc ttcaataatt gcggaagaag ctccgggcgt ctataagccc agtgaagaag
     2701 ttgttgacgt tgttcacaag cttggcatag ctagaaaagt cgtgaagttt atgcccttag
     2761 gagtggcgaa gggatagcta aagaatggtc actttatatt cgccagcaat tcctgcaatc
     2821 ctttagaatt aagcctgcat ttatccccat ctacctgtgc atagcactcc tttatcagaa
     2881 aatcaagctg gaattttaac tgtgattcgg atatcttaag ttctttcatt atttcatttt
     2941 ttgttttccc gaaagctcca atggatttta caatatttct cctcataggg ttctcaagcg
     3001 ccctgaacat catcctgtgg tcatcaggcg tttttatctc gcaagcatcg gattcctgtg
     3061 gaattggttc attctcgtct ttcataaatc gtgccccagt tattaatatg tcatcttacc
     3121 tattatagtt cttcgcttaa caattggaac gtggaaaagt tatccaaata gaatccttca
     3181 tatatcctta taaatatatg aaccctcatg gatacctcgc tgcttcataa ggaaaacgca
     3241 ccgggaattt acaaatgtgc gatattgacc ataagtacct cacgatttga aaaatatggg
     3301 aaaacagacc gccccgaacg cgctgaggat gtatcgggaa aactcatatg gggaatggtg
     3361 caggtcaagg gcagcactat tgttaattac gaattgatcc cggataacat ggaaataatt
     3421 agagaggcct tcaagaaaga cctatattca gaagcggaag tgataatatc aagcgggggc
     3481 acaggccttt cgccaaatga tgtcacaatt gaagccgtga cacccttcat tgagaaagag
     3541 atgcccggat ttggtgaact cttcaggctc cagagcataa atcagatcgg gaattccgta
     3601 atgctcacaa gagctatcgc cggcgttgcg cggggaaaaa taatattttg ccttccaggt
     3661 tcgccaaatg ccgtaaaact tgctcttgaa cttattcttc cggagatggg tcacatcctt
     3721 aaacatttaa agggacagta aatggaatac aaatggaagg cgatgggagt tgtctggata
     3781 ggcatcttta tggctacgct tgacgggagc atagtgaata tagctcttcc aacacttaca
     3841 aagtttttca aaactgatgt cacaaccatc gaatgggtgg tgatggctta tctattgaca
     3901 ataacaagcc ttcttcttag ccttggcagg atatcggaca tggtgggcag gaaaaatatt
     3961 ttcgcgggag gactggttct cttcaccata ggctcatggt tatgcgcctc atcaacaaca
     4021 gagtggcagc taatattttt cagggtattt cagggattcg gggcagcaat ggtgatggcg
     4081 acgggagtgg caataattac ccattcattt cctccgcgtg aaagaggaaa agcaatggga
     4141 ctaataggta cggtggtttc cataggctcg ctggcaggtc cggtaatggg tggattcctg
     4201 atccagaatg tagggtggca atcgattttc tacattaata tacctgttgg gattttcggg
     4261 actgtagtgg cgctaaaggt tcttcaaaaa gaagagacta ttaataaaga ccagacattt
     4321 gatattcctg gcggagttac attgtttatc agcttgatct cattgctcct tgccttaagc
     4381 gagggacagg aaaaaggttg gaactccagc ttcatcatat ccttatttgt atcgtctatt
     4441 gtatttttca ttttatttgt caggatcgaa acaacagcaa aacaacccgt tcttgatctt
     4501 aagcatttca ggaacaggca atttgcatca gcgaacatca gcgctcttat aagtttcatg
     4561 gcaatgttca gtgtcatact tttgatgcct ttttttctcc aggaccaact cggttattca
     4621 acagagaaaa tgggatttgt tttcatgtca gtcccgcttg taatgtctgt agtatccccg
     4681 ttgagcggct ggttatcaga caggacaaat tcgcacgtcc tgagttcaat tggcatcggg
     4741 gtcgcagcct tatccatcct gtcacttggt tatttatctt tggattcaag ttttcccgat
     4801 gtagctcttc gactttgttt tcttggtctt ggcatggggc ttttccaggc gccgaataat
     4861 agcataataa tgggttcgct gcctaaagaa cagcttggca ttgcagcagg ggtaatggga
     4921 acgatgagga atatgggtat ggtcataggt gtcgctgttt caggggcagt attctcaaac
     4981 aggtttgtat tttatgggaa tgagttttta ccggctttca gggatacata tatcgtttct
     5041 gcaattattt gcggtattgc ggttctaata tcacttgtgc gcagcagatc gaaaattgaa
     5101 tgattatctt acagacctgt gatgctgtga cctgaggatt aacacatata cacaaagcat
     5161 cagtccgccg atcgcaaata gaaaaagtaa tataaacgaa gaatattgat agaaataaat
     5221 tgcaatgctt gttcctatca ccattgctat tggaagcaaa aaactaagaa atgttacact
     5281 ttttgatacc atattcagtc tctaatatgg tagtaattgc ttctcaagga taaaatcaaa
     5341 ggtgtgaaaa atagtgtgat aataatttct taaatcatcc tgaaaacaaa gggtattttg
     5401 cgattatacg tattcttatt aattaaaata ccaaaaaaaa tcataatata tacattttaa
     5461 taattaaaag cataataatt tgtttttatt gaaatttatg ttgtcaaaaa ttttaaaata
     5521 ataatatatt cttcacacta aaaactattc acacatattt cacaactccg tttatattca
     5581 ggtgaaccat ttgaagacat agcgctattt aggacgtctt tatgtcggta agaaaaaaca
     5641 tttcattaga aaagggacat ttaaggaaac tggagccact tgtccttaaa cacaagggga
     5701 attttagcgc tgcgatgcgt gaaataatag atcttatcga tacaatgacc agggatcctg
     5761 aagctataaa taaccttata gatggattga aaacagatta taacttaact gaaaacgtga
     5821 ttttctggtt gataaaacag gcgcatggga ggcttattga tccggaacac ctgaacagta
     5881 ttattgaccc gtcaaagatc gttctgcttt ctgaccttga aaagtatatg gatgagatga
     5941 caaatggggc gagctggcag acaaatatta aaatagaaga ctacgatgat aatgtaaatc
     6001 catcacatgc aacggttata ctttcaggaa atagtaatta taaaatggaa tttctcggtg
     6061 gtttaatatc atcattctta gtattgcata agaaacagga gatcatttct ttaaagaaaa
     6121 tgtctaataa gatcattgtt aatttcagaa aacagatgaa tgcaaatctt gcaagacaat
     6181 cacttattaa ttattttgga gatatggaag acctgtcctc tgagattaat aaaaaactgg
     6241 atttctggaa atgcattgta aatctttaca aacagaccaa ttacaacatg gtcacaattc
     6301 ctaagaatta ctataatgaa ttgttgatcg gaaaagaggc gcccagttat cttactgctc
     6361 ctattgaagc aatacataag ataccgataa gagatatacc tctggatgta cttatcccga
     6421 cgatgaaatc ggtttatgag tacatgggga tcgtggaaag aattgacgtt aacgagaata
     6481 ctttgtatat ctatcacgga aataccgata aacgtgcgat aagagcaatt gaaaaaatac
     6541 ttctcaatgt ccttaattcg aatggtatta tatttgaatc acggtgttcg gaaaacctga
     6601 tagtgatgag ccctgtcgtg actaaagctg aaatggttac tatcacgaaa tgatttaagt
     6661 ataattaagt aattttatgt atgcttctgg agaaaaactg gaagcagaag ggtacaaaaa
     6721 atggcaaata tgagcgaaga tgcgttattt aaagcgctgt taaagaacaa accaccacag
     6781 cagccgccaa aacctgcggt tgttgcgcaa agcgttccaa gaaaagaggt cccaagacct
     6841 gcagcagcac cacctgtaga aagataccag gtggaagaac ctgtgatcga accaaaggta
     6901 attcctgtgg ttaaaccaat gaacctggag cctgttgtgg attcaataaa ccggttaaat
     6961 tccagtatta gtatgattca cgggatgatg aaaaccatcg ttaccccaat tctcatattg
     7021 atattgatcg tattaatcgg aatactgata cagactaaat gatacagact aaaataatat
     7081 atattaaata atgcatatta cataatagca gtgggattaa ttagaatatt gtgatgccat
     7141 catcagtgac aatttgatct gttcatttac attattcttt acaacatctc catgacccgg
     7201 gtacatgatg cccacatcaa ggagtg
//
LOCUS       DATGBN010000164         6605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_478126, whole genome shotgun sequence.
ACCESSION   DATGBN010000164 DATGBN010000000
VERSION     DATGBN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478126"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1390
                     /locus_tag="VKL21_03855"
     CDS             308..1390
                     /locus_tag="VKL21_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMB44938.1"
                     /translation="MNNPESKSLNDQELAEKINPFFSIYDIQHAQGNIFFFGTPKEDI
                     NILFQKLWPFFSENGFQFTMKYDLGEHILIASPFSPAKERRWINVVLAVATIFTTMIW
                     GSLLFGANPTASPVEILKGLPFTIAIMTVLGAHEAGHYLVARKHGMHTSLPYFIPFPN
                     MIGTMGAVIKHRGPIPNRKALFDVGVSGPLIGLIVSVIVTIIGLLQPPIEHTQQVLQI
                     ELGTPLLFELIKSVIPGSDGMIMHPIAFAGWVGMFVTALNLIPAGQLDGGHVLRAMLG
                     EKAKEVSRVMPFILISLGFYVTYILNRDGFIWVLWGIFLSFFAASGHPKPLDDDIPIG
                     KGRMALGIITFILGLLCFSLVPFQLQ"
     gene            complement(1393..2550)
                     /locus_tag="VKL21_03860"
     CDS             complement(1393..2550)
                     /locus_tag="VKL21_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44939.1"
                     /translation="MDILDTSSHTKEELVRFLSKELDENPILMLTGPQGGGKTTLAIK
                     LLSENVGAYFEGRGRAAQPVVLIRKDLVEKMKGELYERRKLPIYEDDGPVFVDVSVYD
                     QIRLLVENIFDVMELGELELVKQINLLTNKMGVVPKAIELVASDEELVKRRLARHLDA
                     KERLSTLIEKEKKFGIESNLWGIQGAKITDIINREGVTDYDEDQISRTIKDFDRIIPT
                     KDMTLADCLQNMIEISNNENNESGFLVEHSDDGDKIISDLVVGKGANSLIGVTLLEIY
                     VGFRQNRSLYRAYKHAKEGKLEVIHSYTPQLQEFEYNANPWNPPLTIEIPILSTADIA
                     ASERFDIMVSVIKPDGSVESSSKKRTEELEEIIEGIIWDEDSPNTEDIFED"
     gene            complement(2589..4244)
                     /gene="thsB"
                     /locus_tag="VKL21_03865"
     CDS             complement(2589..4244)
                     /gene="thsB"
                     /locus_tag="VKL21_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HMB44940.1"
                     /translation="MSGQLAGQPIFILREGSQRTKGREAQNNNIMAAKAVAAAVRTTL
                     GPKGMDKMLVDSMGDIVITNDGATILKEMDIEHPAAKMIVEVAKTQDDEVGDGTTTAA
                     VLAGEFLKNAEELLELGVHPTVIASGYRLASIKAKEILKSLSKPVTVDDRDLLLKIAA
                     TAITGKGAESSKDVFAKLAVDAVLAVVDTENGKKVVDVEDIKVEKKVGGSVEDSQLIE
                     GMVIDKERVHTNMPKKVTSAKILLLNEALEIKKTEVDAEISIKSPDQLQLFLDQEEQM
                     IHNMVNKVIDCGANVVFVQKGIDDIAQHYLAKAGIYAARRVKKSDMEKLARATGAKIL
                     TSLKEINDSDLGKAGLVEEKKIGDEAMTYVTECHNPKAVSIILRGGTEHVADEAERAL
                     HDALRVVGVAIEDETLVAGGGSPEIELALRLREYSSTLKGREQLAVAKFADALEVIPR
                     TLAENAGLDPINMLVEMRSQHEKGKKTAGLNVFTGKVVDMWAEGVVEPLRVKTQAISS
                     GTEAATMILRIDDVLSSKSSPGGGMPPGGMGGMGDMGGMGGMD"
     gene            4454..5011
                     /locus_tag="VKL21_03870"
     CDS             4454..5011
                     /locus_tag="VKL21_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMB44941.1"
                     /translation="MEEITFKTEDGVEIAGNYFKPNKEHSPVFLLLHMMPSTKESWNS
                     FASLLQDNGFAVLAIDLRGHGKSIDKNGMRLDYKEFKDPQHRESMKDIAAAKEFLAGK
                     KNVDISRLAIAWASIGANLALWQASLDKDVRLLILLSAGLNYRGIQADQLAPAFSGHV
                     YMLASEGDTFEAAPRFQRKKRFLTI"
     gene            complement(5045..5320)
                     /locus_tag="VKL21_03875"
     CDS             complement(5045..5320)
                     /locus_tag="VKL21_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HMB44942.1"
                     /translation="MIELIWDEKFKRLFKKWSQKHPDLMEQFRNKMELFVIDPFHPSL
                     KTHSLSGILKGSWAMRINYEQRLIFRFVDKEKKKVMLIDLGTHNEVY"
     gene            complement(5313..5519)
                     /locus_tag="VKL21_03880"
     CDS             complement(5313..5519)
                     /locus_tag="VKL21_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44943.1"
                     /translation="MVTLDKVLEETEHLEFDDREYLLNILSKRQIELRRVEISKRVKE
                     ALKAYKEGNVKSGKLDALWKDLND"
     gene            complement(5873..6223)
                     /locus_tag="VKL21_03885"
     CDS             complement(5873..6223)
                     /locus_tag="VKL21_03885"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HMB44944.1"
                     /translation="MKVKFLVDECTGKRLSNLLNAAGYDVIFVGDWKPSASDEEVLDK
                     ANKERRVLITDDKDFGELVFRLEKPSSGVILIRTSTTNSATRMHLLEILLKSTEVYKK
                     FIVLKDKVFKIRDT"
     gene            complement(6220..6450)
                     /locus_tag="VKL21_03890"
     CDS             complement(6220..6450)
                     /locus_tag="VKL21_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMB44945.1"
                     /translation="MEERIVVNPKIMVGKPIIKGTRIPVDAILKRLADGMSISDILEE
                     YPNITEEDIRAALTYVSEVIAGEQIIPMVETA"
BASE COUNT         1742 a         1490 c         1261 g         2112 t
ORIGIN      
        1 tgatcatgaa atataagtca aataggggct tgatagatat tctcctggga cttgtaaaag
       61 ctgtgggtaa acattataaa gaagatctta aggtcacaaa actttcgaat gataaattac
      121 aggttatttt tcctaactga atagcaaccg ggtgttttat ccttaatcag gatgaaaaca
      181 cttatttttt agatattcat tcatgttcgt agtgtatata cactacaaga ctaacttgac
      241 gctatggaaa gggaggagta attcccagtt agattcatta tagagaatgg agtatattag
      301 gtcatagatg aataatccag aatccaaatc cctgaacgac caagagcttg cagagaaaat
      361 aaatccgttt ttctccatct acgatataca gcatgctcaa gggaatatat tcttttttgg
      421 cacacctaag gaagatataa atatactctt ccagaaacta tggccgttct tttctgaaaa
      481 tggctttcag ttcacaatga aatacgatct tggggaacat attctcatag catcgccgtt
      541 ttcaccggcc aaggagcgtc gctggatcaa tgtggtgctt gcggttgcta caattttcac
      601 taccatgatc tggggctctc ttctttttgg cgctaatccg accgcatccc cggttgaaat
      661 tctaaaagga ttacccttta ctatcgcgat catgacagta cttggcgcac atgaggcagg
      721 acattacctg gtcgcaagaa aacatggtat gcatacgtct ttaccctatt ttataccatt
      781 tccgaacatg atcggcacaa tgggggctgt gatcaagcac cgtgggccca tacccaaccg
      841 gaaagctctt tttgacgtgg gtgtatcggg accacttata ggacttatcg tgtcggttat
      901 tgtaacaata atcggtcttc ttcagccccc gatagaacat acacagcagg ttttacagat
      961 agaacttggc actcctttgt tatttgaatt gataaaaagc gttatacccg gaagtgatgg
     1021 tatgataatg cacccgatag catttgcagg ctgggttgga atgtttgtga ccgctctaaa
     1081 ccttattccg gcaggacaac ttgacggagg tcatgttctt cgcgccatgc tgggtgagaa
     1141 agcaaaagaa gtgtcaagag ttatgccttt tatcctaatt tcccttggtt tctatgtcac
     1201 atatatcctg aaccgggatg gttttatatg ggtattgtgg ggtatattcc tttcgttttt
     1261 tgctgcttca ggacatccaa aacctcttga cgatgatatc cctatcggga aaggaaggat
     1321 ggcattaggg ataatcactt ttatcctggg gttgctgtgt ttttcgctgg ttcctttcca
     1381 gctgcaatga aatcagtcct caaaaatatc ttcagtgttg ggagaatcct cgtcccaaat
     1441 aatcccctcg ataatttctt ccagctcctc tgttctcttc ttgctcgaag attctactga
     1501 accatccggc ttgatcacag aaaccattat atcaaatctc tccgaagctg caatatcagc
     1561 cgtggacaat attgggattt caatagtcaa aggcggattc cagggatttg cattgtactc
     1621 gaattcctga agctggggcg tgtaggaatg aatgacttca agttttcctt cttttgcatg
     1681 tttataagcg cgataaaggg acctgttctg ccgaaaacca acatatattt caagaagcgt
     1741 tacaccgata agagaatttg caccttttcc caccacaagg tcgcttatta tcttatcacc
     1801 atcatccgaa tgctctacga gaaaaccgct ctcattattc tcattatttg atatttcgat
     1861 catattttga agacaatccg ccagagtcat atcttttgta ggaatgatcc ggtcaaaatc
     1921 cttgatcgtc cttgatatct ggtcttcatc ataatctgtc acaccttcac gattaatgat
     1981 atcagtgatc ttcgcgccct gtatacccca gaggtttgac tctataccaa actttttctc
     2041 tttttcaatg agtgttgaaa gacgttcctt tgcatcaaga tgccttgcaa gtcttctttt
     2101 gacgagttct tcatcgcttg ccacaagttc gatagccttt ggaacgacac ccattttgtt
     2161 tgtgagtaag ttgatctgct ttaccagttc cagttccccc agttccatta catcaaagat
     2221 attctcaaca agcaacctta tctggtcata gacggaaaca tcaacaaaaa caggtccatc
     2281 atcttcataa atgggcagct ttcttctttc ataaagctct cctttcattt tttcaacgag
     2341 atctttcctg ataagaacta caggctgggc cgcgcgcccc ctgccttcaa aataagcccc
     2401 gacattctcc gaaaggagtt ttatcgccag agtggtcttt cctcctccct gggggccggt
     2461 tagcattaaa ataggatttt catccagttc ttttgagaga aaccggacta attcttcttt
     2521 ggtatgtgaa gatgtatcaa gtatatccat caggatcact aaaaaaaaga agcgggaaaa
     2581 acccgccttt aatccattcc gcccatgccg cccatatcac ccataccgcc catacctccg
     2641 ggtggcatgc cacctcctgg cgatgatttg ctggacagta catcatcgat gcgaaggatc
     2701 attgtggcag cttcagtgcc cgaactgatt gcctgggtct ttactctgag aggttctaca
     2761 acgccttctg cccacatatc cacaactttg cctgtgaata cattaagacc tgctgtcttt
     2821 tttcctttct catgctggct tctcatttca acgagcatgt tgatggggtc aagacctgca
     2881 ttctctgcaa gtgttcttgg gattacttca agagcgtctg caaacttggc aacagccagc
     2941 tgttccctgc cttttaatgt gcttgagtac tctcgcaagc gcaatgccag ttcaatttca
     3001 ggtgagccgc cgcctgctac aagtgtctca tcttctattg caacgcccac aacgcgaagt
     3061 gcatcatgta atgcgcgttc tgcttcatcg gctacgtgtt ctgttccgcc gcgaaggatt
     3121 atcgaaactg ctttgggatt atgacattct gttacatatg tcattgcttc gtcgccaatc
     3181 ttcttttctt cgacaaggcc tgcttttcca aggtcggaat catttatctc cttgaggctt
     3241 gtcaggatct tggcgcctgt agcgcgtgcg agtttttcca tatcgctttt ctttacacgc
     3301 cttgctgcat atattcctgc tttggcaaga taatgctggg cgatatcatc aatgcctttt
     3361 tgcacaaaca cgacatttgc gccgcagtca attactttgt tgaccatgtt atggatcatt
     3421 tgttcttcct ggtcaaggaa taactggagc tggtcgggtg attttattga tatctctgca
     3481 tcaacttcgg tctttttgat ctcaagcgct tcattaagaa gaaggatctt cgcactggtg
     3541 actttctttg gcatattggt atggactctt tccttatcaa ttaccatgcc ctcgatcaac
     3601 tgtgaatctt caacacttcc accaactttc ttctccactt ttatgtcttc gacatccaca
     3661 acctttttgc cgttctcggt atcaactact gcaagtacag catctactgc aagtttcgcg
     3721 aaaacatctt tggatgattc agcgcccttt ccggttattg ctgttgctgc aattttcaat
     3781 aataggtctc tatcatctac tgttacgggc tttgaaagtg atttaaggat ctctttcgct
     3841 tttattgagg caagcctgta tccgcttgcg ataactgtgg ggtgtactcc cagttcaaga
     3901 agttcttctg cattctttaa gaattcgcct gcaagaactg ctgctgttgt tgtgccgtct
     3961 ccgacctcat catcctgggt ctttgcgact tcaacgatca tcttggctgc cggatgctcg
     4021 atatccattt ccttaagaat ggttgcgccg tcgttggtga taacgatatc tcccatagag
     4081 tcaacgagca ttttatccat gccctttggt cccagtgtag tccttactgc tgctgctacg
     4141 gctttagctg ccatgatgtt attgttctgt gcttctcttc cttttgttct ctgactgcct
     4201 tctcttaaaa taaagatggg ctgtcctgct aattgtcctg acataaatag cctccgttaa
     4261 aatttttatt tgataatctg tacaaataca aatgattgta cgagagattc tcgttgtttg
     4321 taattctata taagcgtttt gattagttat attatattat tgatacgata caattatatg
     4381 gtttttgaaa aaaaataatc gaaaataaat atacctacat cgaattatct gaagtataaa
     4441 tgggatgatg caaatggaag aaatcacttt taaaacagaa gacggagtgg aaatcgcagg
     4501 gaactatttc aaaccgaaca aagaacattc accggttttt ctattattac atatgatgcc
     4561 ctctacaaaa gagagctgga actcatttgc atctcttctc caggataacg gtttcgctgt
     4621 actggcgatc gatttgcgag ggcatggcaa gagtatagat aagaacggca tgagattgga
     4681 ttataaggaa tttaaagatc cccagcacag ggaatccatg aaagacattg cggccgctaa
     4741 ggaattcctg gcaggaaaga agaatgtgga tatatccagg ttggcgatcg catgggcaag
     4801 tattggcgcc aaccttgccc tgtggcaagc atcgcttgac aaagatgtca ggctcctgat
     4861 cctgttatcc gctggcctta attacagggg aatacaggcg gaccaacttg ctcctgcgtt
     4921 ttccggtcat gtctatatgc tggcaagtga aggagataca ttcgaggcgg cgccgaggtt
     4981 tcagcgaaaa aaaagatttt taacgatttg aaataattta atatggaagt gtgtccactt
     5041 aatgttagta tacttcatta tgagttccta aatctatcag catgaccttt tttttttctt
     5101 tatccacaaa tctaaatatc aacctttgtt cataatttat tcgcattgcc caggatcctt
     5161 ttaggatgcc gcttaagcta tgtgttttca atgatggatg gaaaggatcg atcacaaata
     5221 attccatttt attcctgaat tgctccatta aatcaggatg tttctggctc cactttttaa
     5281 ataatctttt aaacttttca tcccatataa gttcaatcat tcaggtcttt ccatagcgcg
     5341 tcaagcttcc cgctctttac atttccctct ttgtaagcct tcaatgcttc tttcacccgt
     5401 ttagatattt cgacccttct taactcaatc tgccttttgg aaagaatatt caatagatac
     5461 tcccgatcat caaattccaa atgttctgtt tcttcgagaa ctttatcaag ggtaaccatt
     5521 ttgttcatca ttttatcatg atatttcttg tgtgccttac tatttaaata aatccatttc
     5581 gcggacatgt ctattccatt acgcgccgga aatcaacaga ttcgaggcgc gccgatgctt
     5641 ccccgaaaat cgagatatct agcaatttga aaccgcggat gaacgctgat gaacgcagat
     5701 gtgaaatcac attccggcgc aaaattattg gaaaagacac ataccacatg caactacaca
     5761 tcccgatata agcaatactc gaaatggtta tgatcaaagc cttttatatt tatattaagg
     5821 agaaaatgga ggcaattccc aaaccaaaga tttccagaag aggcatctac attcaggtat
     5881 cccttatctt aaaaaccttg tctttcaaaa caataaattt cttatagact tctgtcgatt
     5941 tcaacaatat ttcaagcaaa tgcatccttg tagcactatt tgtggttgaa gttctgatca
     6001 atattactcc acttgatggt ttctccaatc tgaaaacaag ttcaccaaaa tccttatcat
     6061 cggttatcaa gactcttctt tctttgtttg ctttgtccag aacctcttca tcagatgcag
     6121 atggtttcca atcacccaca aaaataacat catatcctgc agcattcaat aaatttgaaa
     6181 gtctcttgcc tgtgcactca tctaccaaaa atttgacttt caagccgttt ccaccatcgg
     6241 tataatttgt tctccggcta tgacttcaga aacataagtt agtgctgccc tgatatcttc
     6301 ttctgtaata ttcggatatt cttccaggat atcgcttatg ctcatcccat ccgccagtct
     6361 tttaagtatg gcatctacag gaattctggt acccttaata ataggttttc caaccattat
     6421 tttggggttt actactattc tttcttccat ctatataata gttggattca aatctattat
     6481 agttttgtgt gtttatttcg acatggtttg aaataagctc ccatgcggcg cgcccctatg
     6541 tcctcatagc cgccccggga ttacgtccgc tatgacacgc cgggaatcaa gcgagtcgat
     6601 gcgcg
//
LOCUS       DATGBN010000165         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_90543, whole genome shotgun sequence.
ACCESSION   DATGBN010000165 DATGBN010000000
VERSION     DATGBN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90543"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..606)
                     /locus_tag="VKL21_03895"
     CDS             complement(409..606)
                     /locus_tag="VKL21_03895"
                     /inference="COORDINATES: protein motif:HMM:NF021634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB44946.1"
                     /translation="MKLVLDSKLDQGDFDALLLLLQKHVHLVTKEKYDKKMAIAEDIL
                     GNIDITDSPFLALALAFNCSI"
     gene            complement(606..821)
                     /locus_tag="VKL21_03900"
     CDS             complement(606..821)
                     /locus_tag="VKL21_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44947.1"
                     /translation="MVNIVLKFPDDLSWIIKNKEGLKWVEAAIINRLTDIKIGNLMAE
                     KSELKDEDIDEIDHKVKRALYKTIKAE"
     gene            1310..1501
                     /locus_tag="VKL21_03905"
     CDS             1310..1501
                     /locus_tag="VKL21_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMB44948.1"
                     /translation="MPKEIFKRIVCDPDILSGKPVIQGTRISVEFVMELLANNWTHEE
                     IMENYPQIKNGKRVEPNWG"
     gene            1607..>2022
                     /locus_tag="VKL21_03910"
     CDS             1607..>2022
                     /locus_tag="VKL21_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMB44949.1"
                     /translation="MVSHDIIIVGGGLAGLRAALSVRSADVAVISKVHPLRSHSIAAQ
                     GGINAVLGSNDNWEDHAFDTVKGSDYLADQDAVEVLCWEAPSRVIEMENWGTLFSRTE
                     DGKIAQRPFGGAGFPRTAYAQDRTGHHLLHTMYEQAL"
BASE COUNT          570 a          413 c          421 g          618 t
ORIGIN      
        1 ttttgatatc atatcaaatt ttcgaaggct tagtaccctt aattccttcc aagtttcata
       61 aatataaatc ccaagctcaa gaagcatcac ctgcacatcc tcatttaaat tcttcaaaag
      121 gcgtttcttc cctcagatgg cataccccgc tccctttctc ctcatgataa aaaaacgccc
      181 tacggatgta atgtcgagta ttgcccatat cgggatgtgt ggttgcatgt gtacatatcg
      241 gcgttcttta tatagtttga ttcccggcgc gtcacagcgg atgtaatccc ggggcagcta
      301 tgaggaaatg gggggcgctg catgagaatt ttaactgata tttttcatca ggtctttagt
      361 tgtatacacc acaaccttat tttgctgttg aaaatgtcca tcattgcttc agattgagca
      421 attaaaagcc aaagcaagag ctaagaacgg tgagtccgtt atatcaatat tccctaaaat
      481 atcctcagct atagccatct ttttgtcgta tttttctttt gtaacaaggt gaacatgctt
      541 ctgtaatagt aagagaagag catcaaaatc cccttggtct aatttggaat ccagtactaa
      601 tttcattatt cagcctttat tgttttataa agagcgcgtt ttactttatg atcaatttca
      661 tctatatctt catctttcaa ctcgcttttt tctgccatca gatttccaat ttttatatcg
      721 gtcaacctat taatgatagc cgcctctacc cattttagcc cctccttatt cttaattatc
      781 caacttaaat catcagggaa ttttagcaca atatttacca tttttcacct tgccaaggca
      841 ttttattttc gctgaaacct cggtgctcat cgaatcggtg gatttccggc gcgaaaaaat
      901 gcagcggatg taaaccctga acgggcatgg gggcgtgccg cctggggatt tcatgaaagc
      961 tgcggttcag attccataaa tcctgctatc agttcaaaat gaatctcttg ctctgatttt
     1021 gattcatgtc gattgcctcg atagcatgtt tgcgtctgaa atataatatt tcaaaagtaa
     1081 taaatatgtg gcgcttaacc tgaaaaacca gctatgagct ttgtgattgt acacggaacg
     1141 cacgaatatg tgaaaatccg tccaaccgtg ctatcagtgt tcaatgacgg gagccacata
     1201 ttgcgaagag tacaaatacc tcaaagctca ggctttttat cattcatgct tgattgatta
     1261 ctaacaagac ttttatataa gcttaattaa aatgtatgat tggattatca tgccgaaaga
     1321 aattttcaag agaatagtat gtgaccctga tattctcagt ggcaagcctg ttatccaggg
     1381 aaccaggatt tctgttgagt tcgtgatgga actgctcgca aacaattgga ctcatgaaga
     1441 aatcatggaa aactatccgc agataaaaaa tggtaaaaga gttgaaccaa attggggatg
     1501 aatatggaaa gaaaaatggg acttcttaag ccctgatatc gtttcgggaa tgtttttgct
     1561 gaatcaaaaa caataattat ttgttattgc taacctttgt acatctatgg tcagtcatga
     1621 tatcatcatc gttgggggcg gtcttgcagg attgcgcgct gctttatccg taagatccgc
     1681 tgatgtagcc gttatatcaa aagtccatcc cttgcgttcg cattctattg ccgcacaggg
     1741 gggaattaac gctgttcttg gaagcaatga caattgggaa gaccatgctt ttgataccgt
     1801 aaaggggagc gattatcttg ctgaccagga tgctgtggaa gtactatgct gggaagcgcc
     1861 ttcgcgggta atagagatgg aaaactgggg aacgcttttt tcaaggacag aagacggtaa
     1921 gatagcacag cgccccttcg ggggggcggg attcccgcgg actgcgtatg cacaggacag
     1981 gacaggtcac cacttgttgc atacaatgta tgaacaggcg ct
//
LOCUS       DATGBN010000166         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_90587, whole genome shotgun sequence.
ACCESSION   DATGBN010000166 DATGBN010000000
VERSION     DATGBN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90587"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..336
                     /locus_tag="VKL21_03915"
     CDS             43..336
                     /locus_tag="VKL21_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44950.1"
                     /translation="MSSADIPVPASILKKKSSIARIVDTTRHTMEKNYNDDGSSTNSI
                     VKRKIPLTKALVAVQVHPEKRFAYTGKVYEHDDNFADESERHYSGKKKVPLQK"
     gene            598..2142
                     /locus_tag="VKL21_03920"
     CDS             598..2142
                     /locus_tag="VKL21_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMB44951.1"
                     /translation="MTEKYLIYVRQNWKKIGFSIVILIFLISLAIFLLHIIVQTNSSL
                     ALSNKTVDAHVEINQTIQSYSSFKPSNTTVSVAGDGSGNFTCDGSDDQVEINQAFAYV
                     AENPQFTTVYLKGPNTYVISDSIFIGNNMTLEGDPTAVIKLIDNADWPVFKPLITQMD
                     ITGIHGVIIKGFEINGNHDNNLDKKKGFGYYDMTHFHNAKDIQVHDMYMHDGHGEGLV
                     VKIGSNIQFYNNTVYKTGHNALYVEECQNVDAWNNKITVRTDSGLKSKDSNHIKFHDN
                     VINSFYDWSAGGAGILISKATVIVDDVEVYNNNIQDTYGPGIWLIGSSKSYTKDEAKN
                     VHIYHNFLHNTGTNPSIDWVGGILTSGFYDTLIENNTIDGAYHGAIIHMYPIGGNIHI
                     YPTYNNTGHIDLSPKDTGYITIVRNNIIANTQERKKDPDRTGYGVINYLPKTHTFVLE
                     NNCLYNNYAGNYLNCTSTTDIYVYPLLLNQTIGAGYNTSSDSSIELPGNVNLTNIKSV
                     LTHSRP"
     gene            2422..2669
                     /locus_tag="VKL21_03925"
                     /pseudo
     CDS             2422..2669
                     /locus_tag="VKL21_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558061.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          922 a          478 c          508 g          761 t
ORIGIN      
        1 aaacagctac gctgttgaag ctatcgaagg atgtgtggct tcatgagctc agctgacatt
       61 ccagtacctg caagcatatt gaaaaagaaa agcagtatcg cccgcatagt cgatacaacc
      121 aggcacacaa tggagaagaa ctacaacgat gatggtagtt caactaacag cattgtgaaa
      181 cgaaaaattc ctctgactaa agctcttgta gctgttcagg ttcatccaga aaagagattt
      241 gcgtatactg gaaaagtcta cgaacatgat gataattttg cagatgaaag cgagcgtcac
      301 tattcaggta aaaaaaaggt tccgttgcaa aaataatttt gctgcctttt taggctataa
      361 ctattgcttt ttgtatcatt attgacctgt ttatggcatc taaatatccc atttgtgatg
      421 tttttcactg gttttgtaaa atagccagat tttttacaac ggaacccttt ttattgtcgt
      481 ctgatttttt gatttattgc agtcaataac caatggaatt aatgttagat tttgattatc
      541 cctgagtatg ttaaaaagtc tccaagatca attttatagc taaatcggag gaataaaatg
      601 accgagaaat atcttatcta cgtacggcaa aactggaaaa aaataggatt ttctatcgta
      661 attctaattt tcttgatttc actggcaata ttccttcttc atatcatagt gcaaaccaac
      721 tcttcccttg cattatcaaa taagactgtt gatgctcatg tagagataaa tcaaacaata
      781 cagtcctact cttcttttaa accgtcaaat acgactgtga gtgtagctgg tgatggaagt
      841 ggaaacttca catgtgatgg gagtgacgat caggtagaga taaatcaagc ttttgcatat
      901 gttgcagaaa acccgcagtt tacgacagtt tatttgaagg gtccgaatac atacgttatc
      961 tcagatagta ttttcatagg aaataatatg accctggaag gagatcccac agccgtaatt
     1021 aaactcatag acaacgcgga ctggccagta tttaagcccc tgataacaca gatggacatc
     1081 accggaatcc atggagtcat cataaaagga tttgaaatta acggaaatca tgacaataat
     1141 ctagataaaa agaaggggtt cggatattac gatatgaccc atttccacaa tgctaaggac
     1201 attcaagttc atgatatgta catgcatgat gggcatggag aaggattagt agtaaaaatt
     1261 ggttccaata ttcagtttta caataacacc gtatataaaa cggggcataa tgctctttat
     1321 gtcgaagaat gccaaaatgt agatgcatgg aacaataaaa taaccgtcag gactgacagc
     1381 ggtcttaaat caaaggactc gaaccacata aaattccatg ataatgtcat caactccttt
     1441 tacgactgga gtgcaggtgg cgctggaatc ctgatttcga aagcaacagt tatcgttgac
     1501 gacgtagaag tatataataa taatatacaa gacacttatg gacctggaat ctggttgata
     1561 ggtagttcta agtcttatac aaaggatgag gctaagaatg tccacattta tcataatttc
     1621 ttacataata ctggtacaaa ccctagtatt gactgggtag gaggtatatt gacaagtgga
     1681 ttttacgaca ccctcatcga aaacaatact attgacgggg catatcatgg cgcaattatt
     1741 cacatgtatc ccataggtgg taatattcat atttatccta cgtataataa tacaggtcac
     1801 attgaccttt cacctaaaga tacgggatat ataacaattg tccgcaataa tattatcgca
     1861 aatacccaag aacgtaagaa agatcctgat agaacaggat atggagtaat taattatctc
     1921 cctaaaacac atacctttgt gctggaaaat aactgtctat acaataacta tgcaggtaac
     1981 tacctaaact gcacatcaac tactgacatc tatgtatatc ctctcttatt aaaccagacc
     2041 attggtgccg gatataatac ttcatctgat tcttcaatag agcttccagg aaatgttaac
     2101 ttgaccaaca taaagtccgt ccttactcat agtcgcccat gacgcgcctg caactatatt
     2161 gatctgtaga ggcctgaata tcccgaagtc caaggacttc tggatccagg acacttcggc
     2221 tgcaaatgaa aatatgttta tggtgcttgt gatcaggagc ctggagcagt ctggatagga
     2281 gtttacccgg ctgaaacacc tgttaaagaa attcgaaaac taccgaattt ggtcgattta
     2341 ataaagaaca aaggatggtt ccgttgcaaa aaatatctgc taacgatata ataaaataaa
     2401 gaggaaatca aaggtagtga catgctatct aattccttta aatggaagca ctttgtaggt
     2461 gaaactatct tattaaatgt cagatggtac ttaaaatacc cattgagcta tcgaaattta
     2521 aaggaaatga tgatagaaag aggcattcaa gttgatcaca gtaataatga gatgggtgca
     2581 tcaatattct cctgaaatag aaaagaagat tagaaaacat ttaagaccaa caaacgattc
     2641 gtggagagtt gatgaaacct atatcaaag
//
LOCUS       DATGBN010000167         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_129765, whole genome shotgun sequence.
ACCESSION   DATGBN010000167 DATGBN010000000
VERSION     DATGBN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129765"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VKL21_03930"
     CDS             complement(<1..275)
                     /locus_tag="VKL21_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44952.1"
                     /translation="MIVNRVDVEKFKETVEKARKDPATGKKTMEIEGEWRIGKTGPQF
                     ESKIKTEKGGEITLQSDETLILGGGGTAPNPVQYCIYGLIACYAATFA"
     gene            complement(288..611)
                     /locus_tag="VKL21_03935"
     CDS             complement(288..611)
                     /locus_tag="VKL21_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMB44953.1"
                     /translation="MKIGIIINTNEPETVWNAFRFGVTSLLMEHEVKVFLLGKGVESE
                     SIQDKKFNVHEQIALFVEHKGQIFACGTCLKIRQMKGSEVCPISTMHDMLHIVEESDK
                     VLVFG"
     gene            complement(608..928)
                     /locus_tag="VKL21_03940"
     CDS             complement(608..928)
                     /locus_tag="VKL21_03940"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMB44954.1"
                     /translation="MNKKIYEMHAQICKVFTSPKRLEIISLLRDGEKTVNELAEQAGV
                     LQANISQHLTVLRQNNVVTTRRDGANVYYKIANPKILQAFDLMREVLIEKLAENEKLA
                     KRMS"
     gene            complement(955..1671)
                     /locus_tag="VKL21_03945"
     CDS             complement(955..1671)
                     /locus_tag="VKL21_03945"
                     /inference="COORDINATES: protein motif:HMM:NF040157.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HMB44955.1"
                     /translation="MENKKTENCMICGEELAYLTTAIPVTCTYCGKAESANIHCPSGH
                     YVCNECHASDSIKIITQFCLSSSSKNPMEMAKIIMRHPTMPMHGPEHHAMIAGVLVTA
                     YKNLTGKVADDEIKEAIRRGATVPGGYCGLYGADAAAIATGIAISTILKASPLTDYER
                     RTANMMTSRALAAIASNRGARCCKRSTFVALDSAIQYFREVLGTELEYIPVSKLKCEH
                     SFRNKTCSRADCRFYAGKAL"
     gene            complement(1688..2296)
                     /locus_tag="VKL21_03950"
     CDS             complement(1688..2296)
                     /locus_tag="VKL21_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMB44956.1"
                     /translation="MAWYGNWVAVIASILFFTLFAVGFAYAPKKRDWKTLGIYEAFMV
                     ALFTEMFGFPLTIFLLSSILGITIEPTGETGHLIAVALDRMGILGLEKGVFLVMVISS
                     VMILAGLALIILGWKKIHKAQGLVKDGIYRYSRHPQYLGILLITSGFIIQWPTILTVI
                     MWPVLVIMYIRLAKKEEKEMEEKFGDEFRKYKNETAMLFTIS"
     gene            complement(2299..2598)
                     /locus_tag="VKL21_03955"
     CDS             complement(2299..2598)
                     /locus_tag="VKL21_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44957.1"
                     /translation="MSNKDSTLWILIALLVVVVLLFSGGGMMGFGMGSVMGFGAIIMV
                     LFWGAIIWLVISLINAGTKKSEETSESALTILKKRYVSGEVTKEQYLEMETELTA"
     gene            complement(2676..3302)
                     /locus_tag="VKL21_03960"
     CDS             complement(2676..3302)
                     /locus_tag="VKL21_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB44958.1"
                     /translation="MTKNSAEVFDRNWEDYEDWFKRHKSIYFSELKALKKVIPEGLGL
                     EIGVGSGRFAQPLGAKIGIDPSRNMLKLAKKRNIQVILGTGENLPFKDLTFDFILVII
                     TLCFVKNPVGVMKEAGRILKRGGRLIVGEINKDSHLGQLYEVKREKSEFYKLAAFYSG
                     NEIIEMFNKVGIRYAESHQTLMESIDMEEEPKRGADKGGFVVLSGIKE"
     gene            complement(3325..>4305)
                     /locus_tag="VKL21_03965"
     CDS             complement(3325..>4305)
                     /locus_tag="VKL21_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMB44959.1"
                     /translation="KNGVLIKGGNYIEEMAKAKVFVFDKTGTLTEGRPVVTDVLEVNS
                     YSANEILEIAASIESLSEHPLARAVVAKAESGGMSLKSASEFKAIPGKGVKGNIDGKM
                     YYIGSPAMFSGLSIPFPEVKVRELEEEGKTAILLGNRECMGIIAIMDKIRDTAPETII
                     MLKKMGMRVVMLTGDNERIAKAIANKLGIDEYHAGLLPEDKVRIIEELDQKYGKVVMV
                     GDGVNDAPALAKSSVGIAMGAIGSDVALETADIALMHDDISKLPYLLKLSKKTLGIVK
                     ENIFTSIAIKGSFAILAFPGIVTLWMAVAFGDMGLSLLVIVNAMRLSVLK"
BASE COUNT         1118 a         1065 c          739 g         1383 t
ORIGIN      
        1 gcaaaagtag cagcataaca ggcaattaat ccatagatgc agtactgcac agggttaggg
       61 gcagtgcctc cccctcccag tatcagcgtt tcgtcagatt gaagggtgat ctctcctccc
      121 ttttctgttt ttatctttga ttcaaactgg gggccggttt tgcctatcct ccattcaccc
      181 tctatctcca tggttttctt acccgtggca gggtcttttc tcgctttttc aacagtttcc
      241 ttgaattttt ccacatctac tctgtttact atcattcttt cacctcttta accaaatact
      301 aagaccttgt cagattcctc aactatatgg agcatgtcat gcattgtaga aattggacag
      361 acctcgcttc ccttcatctg ccttattttc aagcatgttc cacatgcaaa tatctggcct
      421 ttatgttcaa caaaaagcgc aatctgttca tggacgttga attttttatc ctgaatactt
      481 tcgctctcca cacctttacc aaggagaaac acttttacct catgttccat aagtaaagat
      541 gttacaccga atctgaaggc gttccacacc gtttccggct catttgtgtt tattattatt
      601 cctattttca tgacatcctc tttgctaatt tttcattctc ggctaatttc tctataagta
      661 cttccctcat cagatcgaat gcctgtaaga ttttcgggtt tgcgattttg tagtaaacat
      721 ttgctccatc ccttcgtgta gtgacaacgt tattctgcct caaaaccgtg agatgctgtg
      781 agatgtttgc ctgcagcacg ccggcctgtt ctgcaagttc attaacggtt ttttctccat
      841 ccctgagaag gctgattatt tccagccgtt ttgggcttgt gaagaccttg caaatctggg
      901 cgtgcatttc ataaattttc ttattcatta tgtactcctg ccagattctt catattaaag
      961 cgcctttccg gcataaaatc tgcaatcagc tcgtgagcaa gtcttatttc taaaactatg
     1021 ctcgcatttg agttttgaaa ctggaatata ttcaagttca gttccaagta cttccctgaa
     1081 atactgaatt gccgaatcta acgcaacaaa agtgctccgt ttgcagcacc tggcccccct
     1141 gttactggct atggcagcaa gcgcacggga agtcatcata tttgcagttc gtctctcata
     1201 gtctgtcagg gggctggctt ttaatattgt gctaatagca atgcctgtgg ctatagctgc
     1261 tgcatctgca ccataaaggc cgcaataacc gcccggcacg gtggctccac gccttattgc
     1321 ttctttaatt tcatcgtctg ccactttgcc agtgagattt ttgtaggcag taaccagcac
     1381 tccagctatc atggcgtgat gttccggccc gtgcatgggc atggttggat gcctcataat
     1441 tatctttgcc atttccatgg gattttttga acttgaagac agacagaact gggtgattat
     1501 ctttattgaa tcgcttgcat ggcattcgtt gcatacataa tgaccagacg ggcaatgaat
     1561 gttagcagat tctgctttcc cgcaataggt gcacgtcaca gggatagccg ttgttaaata
     1621 tgcaagttct tcaccgcata tcatgcagtt ttcagttttt ttattttcca ttatttcctc
     1681 cttaaaatca agaaatcgta aataacattg cggtttcgtt tttatatttc cgaaactcat
     1741 ccccgaactt ctcttccatc tccttttcct ctttctttgc aaggcgaata tacatgatga
     1801 ccagaacagg ccacatgata acggtaagta tagtgggcca ctggataatg aatcctgaag
     1861 taataagtaa aatacccagg tactgcggat ggcgggagta acggtagatt ccatctttca
     1921 caagtccctg agctttatgt attttcttcc atcccagaat aatcagagca agtcccgcta
     1981 ggatcatgac tgaacttatt accatcacca ggaatacacc cttctccagc cccagtattc
     2041 ccatacggtc gagtgcaact gctatcaaat gaccggtttc gcctgtgggt tctatagtga
     2101 tgcccagaat cgaagataaa agaaaaatcg taaggggaaa gccgaacatc tcagtaaaca
     2161 gtgcgaccat gaaagcctca tatattccaa gcgtcttcca gtcgcgcttt ttcggagcgt
     2221 aagcaaatcc cacggcaaaa agtgtgaaga acaaaatgct tgctatcaca gctacccagt
     2281 ttccgtacca ggccatgttt acgccgtcag ttccgtttcc atctcaaggt actgctcttt
     2341 tgttacttcg ccactgacat atctcttttt caggattgta agagcggatt cagacgtttc
     2401 ttctgatttt tttgttccgg catttataag tgaaataact agccagatta ttgctcccca
     2461 gaataatacc attattatgg ctccaaagcc catgacagag cccattccaa agcccatcat
     2521 tccgcctccg gaaaacaaca ggactactac cacaagcagt gcgatgagta tccacaatgt
     2581 tgaatcttta ttgctcattt tttatacatc tcttcaattt tagattttaa aatcatattc
     2641 taagatatga atattcacac atatgaattt tacctttatt ctttaatgcc tgataatacg
     2701 acaaatccac ctttatccgc tcctcttttt ggctcctcct ccatatcgat agactccata
     2761 agagtttggt gtgattctgc atatcttatt cccactttgt taaacatttc aataatttcg
     2821 ttaccggaat aaaaagcagc cagtttgtaa aattcacttt tctctctttt aacctcatat
     2881 agttgcccta aatggctatc tttattgatc tcaccaacaa tcagtctgcc gcctcttttt
     2941 aaaatcctgc cagcttcttt catgacacct actggatttt ttacaaaaca aagagtgata
     3001 atcacaagaa taaaatcaaa tgtaaggtcc ttaaatggca gattttcacc ggtacctaaa
     3061 attacctgga tattgcgttt ttttgcaagc tttagcatgt ttcttgaggg atcaatacct
     3121 atttttgctc caagaggttg agcgaatctc ccgcttccaa caccgatctc cagacccaaa
     3181 ccctcaggaa taactttttt cagggcttta agttctgaga aataaatact tttatgtctc
     3241 ttaaaccaat cttcataatc ttcccaattt ctatcaaata cctcagcact atttttggtc
     3301 attctatttt ccaaataaac agtgtcattt taacaccgat agccgcatcg cgttaactat
     3361 cacaagaagt gacagaccca tgtctccaaa tgctacagcc atccagagag tcacgattcc
     3421 aggaaaagca agaatggcaa agcttccttt tattgcgatt gatgtaaaga tattctcctt
     3481 aactatccca agtgtttttt tactgagctt caataaataa ggcagtttgg atatatcatc
     3541 atgcattaac gctatatcag cggtctcaag tgcaacatcg ctgcctatag cccccatcgc
     3601 aatgccgaca ctcgacttcg ccagcgcagg tgcatcgttc actccatcac ctaccattac
     3661 aaccttacca tatttctgat ccagttcttc aataatcctg accttatctt caggaagaag
     3721 accggcatgg tattcatcga ttccaagttt gtttgcaatc gcctttgcaa ttcgttcatt
     3781 gtctcctgtc agcatcacca ccctcatccc catcttcttt agcatgatta tggtttcagg
     3841 cgcagtatcg cgtattttat ccatgattgc aataattccc atgcattccc gattccccaa
     3901 aagtatagct gtcttaccct cctcttcgag ttctctgact ttaacttctg ggaaagggat
     3961 tgatagcccg gaaaacatgg caggactgcc gatataatac atttttccat ctatgtttcc
     4021 ttttacaccc ttgcctggaa ttgccttaaa ttcgctggcg gattttaagc tcatgccacc
     4081 ggattcagct ttcgcaacaa ctgcccttgc aagaggatgc tcggataacg actctatgga
     4141 tgcagctatc tccaatattt cattagctga ataactatta acctccagta catctgtcac
     4201 caccggtctt ccttccgtca gggtgcctgt cttatcaaaa acaaaaacct ttgccttagc
     4261 catctcttcg atgtaatttc cgccttttat taagaccccg ttctt
//
LOCUS       DATGBN010000168         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_168411, whole genome shotgun sequence.
ACCESSION   DATGBN010000168 DATGBN010000000
VERSION     DATGBN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168411"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VKL21_03970"
     CDS             complement(<1..739)
                     /locus_tag="VKL21_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HMB44960.1"
                     /translation="MKLAFLTSFLILTFPILASSVLADTNLVLNSGLESGTTTPLNWT
                     FVNQGENTPIWDNVSHSGSRSIQISIPGTTNSISGYPQSDTIDAQPLTTYKASVWGKT
                     QNTGGANSPAAIVVELDANKNWIRQNNILPVFGRGTNDWTQRTIEFQTGADTRYLYVY
                     ANIWNGYGTFWMDDVIVSLKTSIPIPTPTPIETNLVLNPGLESGTTTPLNWTFENQGG
                     NTPILDSVSHSGSRSIKISIPGITNSIS"
     gene            944..1348
                     /locus_tag="VKL21_03975"
     CDS             944..1348
                     /locus_tag="VKL21_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMB44961.1"
                     /translation="MDCDIENCKSLCCHNCAVLTESEVSNLILNVRKKYSIELEPRKF
                     FKKVKGEIGIYYAIKMIKGQCIFLNKENRCRIYLCRPTLCELYPVIDIDKVDERCPIA
                     NMLPPEALVDLKRRYAEEIDDDIKAEQTFLFV"
     gene            1428..1676
                     /locus_tag="VKL21_03980"
     CDS             1428..1676
                     /locus_tag="VKL21_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HMB44962.1"
                     /translation="MAIELIIVILAIIGVIAAYYLLKTATKLIINAVLGLIILVAANA
                     IFNLDINYSAYVLLVCAFAGIPGAIIVILLHVFGIAFL"
     gene            1713..2414
                     /gene="pyrH"
                     /locus_tag="VKL21_03985"
     CDS             1713..2414
                     /gene="pyrH"
                     /locus_tag="VKL21_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMB44963.1"
                     /translation="MKIIVSIGGSVLAKELQPEKFKGYATVIKEIGKNHTLFIVTGGG
                     KAAREYIGVARDLGSDEATCDFIGIDITRLNARLLIAALGEAAYYEPPLNYKEARNAS
                     LTGKIVVMGGVTPGQTTDAVSAVLSEYVGADLLINATSIDGVYTSDPKKNKDARKFET
                     MTPHQLIEIVMKTEMVAGANSPIDLLAAKIIERSNIKTIVLNGENPQNILDAVSGKYR
                     GTVIEKAKPEKTKKE"
     gene            2433..3290
                     /locus_tag="VKL21_03990"
     CDS             2433..3290
                     /locus_tag="VKL21_03990"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HMB44964.1"
                     /translation="MFENIFKGFIFIKESFILIIKDADVIKPSFYSIFVGMFFSIFSV
                     IIMLFIESNLRLGNLFYLLAFLVFLGNYLISYFFSGMTAFLVYDYFKDGDATMSEAWD
                     ATKKNILTIFYLAILSSIVNIITGVLRGRARRDSGIAGGFSGMILGFIDGAWTVAAYF
                     MIPAIVIEDRDLKGAVERATQIIKKNLLPIGVGEIAVGLVTGILSVIGFIIAIVIGVS
                     LINVIGPVLALLMAAILILIVIALSLYVTTAYHTCLFLWARSVESTGIRGSAMSPPAP
                     IANALGLAG"
BASE COUNT          968 a          585 c          729 g         1012 t
ORIGIN      
        1 ctgatatact attcgttatt ccggggatgc ttatttttat tgatcttgat ccgctgtggg
       61 atacgctatc caagatcggt gtgtttccgc cttgattctc aaatgtccag ttaaggggag
      121 ttgttgttcc actttctaaa cccgggttta aaacgagatt tgtttctatt ggcgttgggg
      181 tgggtattgg aatcgaggtt tttaggctta cgattacatc atccatccag aaagttccat
      241 aaccattcca tatatttgca tagacgtaaa gatatcttgt atccgcacca gtctggaatt
      301 caattgttct ttgtgtccag tcatttgtgc ctctaccgaa tacaggaagg atgttattct
      361 ggcgaatcca gttcttgttt gcatcaagtt ccactacaat ggcagccggg ctattcgcgc
      421 cacctgtgtt ctgggttttt ccccatactg aggctttata tgttgtaagg ggctgtgcat
      481 ctattgtatc cgattgtgga tatcctgata tactattcgt tgttccgggg atgcttattt
      541 gtattgatct tgatccgctg tgggatacgt tatcccagat gggtgtgttt tcgccttgat
      601 tcacaaatgt ccagttaagg ggagttgttg ttccactttc taaacctgag ttcaatacaa
      661 ggtttgtatc tgcaaggact gaggatgcta aaataggaaa cgttaatatt aaaaaagaag
      721 ttagaaatgc aagtttcata acattcttag gtttaaaatt aattttttct aaatacatcc
      781 tcatggattg tattatgata atgctcatca taatgtagag aatgtaatgg acattacata
      841 aggtcatggc cataaaagat gagcatcaaa acaatataac attgtagatg tcacataaag
      901 gtttatggcc atacaagata agcatattaa tgaaatttga tatatggatt gtgatattga
      961 aaactgcaaa tcgctttgct gccataactg cgccgtactg accgaaagtg aagtctcgaa
     1021 tttgatcttg aacgtcagga agaaatattc tatcgaactt gaacccagga agtttttcaa
     1081 gaaggtaaag ggagaaatcg ggatatatta tgccattaaa atgataaaag gacagtgcat
     1141 ctttttgaat aaagagaacc gctgccgcat atatttatgc cgccccacct tatgtgaatt
     1201 atatcctgtc atcgatattg ataaggtgga tgagaggtgc ccgatagcaa acatgctgcc
     1261 accggaggcg cttgttgact tgaagcggcg gtatgcagaa gagatcgatg atgatataaa
     1321 agctgaacag acgttcttgt ttgtttgaac cttttctccg gaataaaata aaaaatctca
     1381 gagaactaat agaaatgttt aattaaaatg agagccatta tagtaaggtg gcaatagaat
     1441 taattatagt tatattagca ataattggag ttattgcagc atattatctc ctgaaaaccg
     1501 cgacaaagct cataataaat gctgtgctgg ggctcattat tctggttgca gccaatgcaa
     1561 ttttcaatct cgatatcaat tattctgcct atgtattact tgtttgtgca tttgcaggga
     1621 ttcccggtgc aatcatcgtt atactgcttc acgtgttcgg gatagctttc ctatgagaaa
     1681 gataataata ttaaagaaca atcagatatt ccatgaagat catagtttca attggcggat
     1741 cggttctcgc taaggaattg cagcctgaaa aatttaaagg ctatgcaact gttattaaag
     1801 agataggaaa aaatcataca ctatttattg taacaggtgg aggaaaagca gctcgtgaat
     1861 acataggagt ggcacgagat cttggttctg atgaagcgac ctgtgatttt attggtattg
     1921 atatcacgcg actcaatgcc agacttctga tagcagctct tggtgaagca gcttattatg
     1981 agcctccttt gaactataaa gaggcaagaa atgcaagctt gacaggcaaa atagtagtaa
     2041 tggggggcgt aactcccgga cagacaactg atgccgtttc tgctgtactt tcagaatatg
     2101 tcggcgcaga tctgctaata aacgcgactt ccattgatgg tgtttatacg agcgacccga
     2161 agaagaataa ggatgccaga aaatttgaaa ccatgacgcc ccatcaactt attgaaatcg
     2221 tgatgaagac tgaaatggtc gccggggcca attcacccat cgaccttctg gccgcaaaga
     2281 taatcgagcg aagtaatatt aaaaccattg ttcttaatgg ggaaaaccca caaaatatcc
     2341 ttgacgcggt aagtggaaaa tacaggggga cggtaattga gaaggccaaa ccggaaaaaa
     2401 ccaaaaaaga atgaattgga aaaggatgta ttatgtttga aaatatattc aaaggtttca
     2461 tctttataaa ggagagtttc atcctgatta taaaagatgc agatgtcatt aaaccctctt
     2521 tctattctat atttgtagga atgttttttt ctatcttttc agttattatc atgcttttta
     2581 ttgaatcaaa cctgcgtcta gggaatttat tttatctact ggcctttctt gtttttctgg
     2641 gaaattattt gatctcctat tttttctcag ggatgacagc atttcttgtt tacgattatt
     2701 ttaaagacgg cgacgcgaca atgtcagaag catgggatgc aacaaagaaa aatatattaa
     2761 caatattcta tcttgcaata ttgtcgtcga tcgttaatat cattacaggc gtcttaaggg
     2821 gccgcgcgag aagagactca ggcatagcag gtggtttttc cgggatgatc ctgggattca
     2881 ttgatggggc gtggactgtt gctgcttatt tcatgatccc cgcaatagtt attgaagaca
     2941 gggacttaaa aggggccgta gaaagagcaa cacagatcat aaaaaagaat cttcttccca
     3001 taggagtagg tgaaatagct gttggattgg ttaccgggat tctttcagtg atcggcttca
     3061 ttattgctat cgtcatcggc gtgtcattga tcaatgtgat aggccctgta ttggcattat
     3121 tgatggcggc gattttgatc ctcatcgtga tagcactttc tttgtatgtc accacagctt
     3181 accacacatg ccttttcctg tgggcaagga gtgtcgaaag cacaggcatt cgtggaagcg
     3241 ctatgagccc ccctgcgccg atcgcgaatg cgctggggct cgctggatga aata
//
LOCUS       DATGBN010000169         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_284501, whole genome shotgun sequence.
ACCESSION   DATGBN010000169 DATGBN010000000
VERSION     DATGBN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284501"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1123
                     /locus_tag="VKL21_03995"
     CDS             347..1123
                     /locus_tag="VKL21_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44965.1"
                     /translation="MVTDPRMYKEFKNNVSLFVGCRNDCVYCRNTFQKQAKRRPCALC
                     KAFEPHEHPERLEKRPNRRHMRLCGFGDISFVSDEYMKKVIEYAKKYPKTEFLVQSKN
                     PSIFLKYEWSDNVILCATLECDGSLVDYGKISKAEPPEKRIDVMKQLHHRKAITVEPV
                     LDFDAKIFPLLIQDIKPEWVWVGYDSRPKKHHLQEPALEKPIELIKTLEDAGITVRKK
                     LIRKAWYEDKAENNSMRKLGDTKERRKKKRQGDNKDNQGR"
     gene            1881..2252
                     /locus_tag="VKL21_04000"
     CDS             1881..2252
                     /locus_tag="VKL21_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44966.1"
                     /translation="MNKYDEILNTKIVKEQINEAMEKSKGGKEHGFYICLNGEDITAT
                     EIVDGNSNSITMENTCPGVKIGSFHVHPMSEIVYPSPKDFANINENDGEKFLCIGANV
                     NGGNGTRKVVQCFDREDSVIL"
     gene            complement(2547..>4412)
                     /locus_tag="VKL21_04005"
     CDS             complement(2547..>4412)
                     /locus_tag="VKL21_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HMB44967.1"
                     /translation="TEAWWRIFHYNNALSQEDSIRIGEYLKKKWVSFQEAIYHHIGEL
                     GLSKSGLETINNVKKRMDSLCGKKNLDVSDLILEVYDLSGLSRQFSHTDTKRSREALL
                     NLRKLHELTSNFEEFHGKDLSLFIDYLEILDEMDGNPPSAKITDEDAVNLMTIHAAKG
                     LEFNIVFLTNLAKDKFPLYRGGTEPLIPPELMDQYMDIFADGSIKDTEKAVRERKKEI
                     KKEEERRLAYVAFTRAKEHLFLTLAVNYGEDEREPSEFLQDLGYDNWRAGGNITVGDL
                     SYFRDTDTKVREMVKDSALEREKAMRKRLIIESLDSGDFNEIMKNTLLYQALKLGKAM
                     EFKEIFNSNWPKINPADDAEKILSKIKDNKNGLKFNPASMTLSYSSISTYEKCPRQYE
                     LKELLRMPTRDSEDSTGAMRRGNFVHSVLETAVSEKIFSKDKLYEIRDMVAKEPDFRG
                     VDIETATGALDVFWERNKDKITNNLMVEQHFTVPLGGFNFKGFIDRVDLISGTKNEIE
                     IIDYKTGKSEPRPDERSKQLLLYARGIEHVYPQYKVKRLTLELLNIPNPRSFELDGGK
                     YECAGSSRMEGLDENAIDGMIEIAKSIAYDYEHGFERTKDEKACAECGYRLYCAD"
BASE COUNT         1372 a          846 c          797 g         1397 t
ORIGIN      
        1 gacagggatt actttaaata aggaatctac tgaactcttg aaagctgaaa aggagaagat
       61 ggataaggat aactttgagt aaaatggctg ctatgcttaa taaatatatt tattaaaaaa
      121 tagtgcttat agaggctata ataatgtatt attatatgag attttcttat atattattta
      181 tttatatatt attattttta ccttaataat aagagaaaaa ggcatctata agcactatta
      241 ataagaattt gacaattgat cgcgatgaac atagccagat attattaaaa ggaattcccg
      301 catgaagaat caagattgat tgtcgttatg aaaaaggaga aatagaatgg ttacagatcc
      361 acggatgtat aaagaattca agaacaatgt tagtttattt gtcggatgca gaaatgactg
      421 cgtttactgc cggaacactt ttcaaaaaca ggcaaagaga cgcccatgtg cattgtgcaa
      481 agcatttgag cctcatgaac atccagagag gcttgaaaaa agaccaaacc gcaggcatat
      541 gagattgtgc ggttttggtg acatttcctt tgtaagtgat gaatacatga aaaaggtcat
      601 tgagtatgca aagaaatatc cgaagactga atttctggtg caaagtaaaa atccttcaat
      661 ttttctgaag tacgaatggt ctgataatgt aatcctctgt gccacacttg aatgtgacgg
      721 ctcgcttgtg gattacggaa aaatcagtaa agcagagccg cctgaaaagc gaattgatgt
      781 gatgaagcag ttgcatcaca gaaaagcaat aaccgtagag ccagttttag actttgatgc
      841 caaaatattc cctttattaa tacaggacat aaagccagaa tgggtatggg ttggatatga
      901 cagtcgaccc aaaaagcatc atcttcaaga gccagcactg gaaaaaccga ttgaattgat
      961 taagaccttg gaggatgctg gaattacagt acggaaaaaa ctcattagaa aagcatggta
     1021 tgaagataag gctgaaaaca acagcatgcg caaacttgga gataccaagg aacgtcgcaa
     1081 aaagaagcga cagggcgaca acaaggacaa tcaggggaga tgatagtata tatttgactt
     1141 cattcgcatg tccccacaaa atgaaaacgg agaatatttc aaaaataaca attggtggtg
     1201 gagaccagtg catcatcctt tcattatgtt cttaataata tcagccgatg tttcaaaatg
     1261 ccatcaacac aaatggtctg ccgtttttct cttttttata agtcattaca gtttgttttc
     1321 tgatcaaaac aagtggaagc actaagacat aaccgaaagt atgtggtttg catctcggct
     1381 caatgaggat gaactattca tttgactgga aacttaaaga aagtaatgac aagctccgat
     1441 agtctatggt cttcaaattt ggcacaacct gattatcatt tttctcgttc ttatattttt
     1501 caaaacactt aatcaaaaac tttcatatcc ttgaacaatt tattaccctt tgtcttcatt
     1561 cttaatatcc ataaacaaat ttcaaggcaa ttagcacagc tacgtcagag tgtgtaaaaa
     1621 ctcaaaattt tgcaaaatag aaagcttatt tttcaattta aaggcttaat aaggcttatt
     1681 ttttaagcaa acatgacttt ttactacttt tcacacagcc tgacgtaatt gtgataaaag
     1741 cagatctatg aaaaacatct actattgtct ttaattatcg caagtatctg ttcaataaaa
     1801 atttaaatag ttaggcgttg tcttatctat atagttcatt atataaaaag ataatgatga
     1861 attgtaaaga aggagaaata atgaataaat atgatgaaat attaaataca aaaattgtga
     1921 aagaacaaat taatgaggca atggaaaaat ccaaaggtgg caaagagcat ggattttata
     1981 tatgcttaaa tggagaagat attaccgcta cagaaattgt agatggaaat agtaattcaa
     2041 taacaatgga gaatacttgt ccgggggtga agatcggatc tttccatgtc catccaatga
     2101 gtgagatagt ttatccttct ccaaaagatt ttgcgaatat aaacgagaat gatggagaaa
     2161 aattcttgtg cattggcgct aatgtaaatg gtgggaatgg aactcgaaaa gtcgtacaat
     2221 gttttgatcg cgaagactca gtgattttgt gattccggtt tttttttgct gctgacttaa
     2281 agaccggcga gacaatctta tacaataatt tatatgattt aattttttta tttttttcat
     2341 ttctacataa ttttttattg aatctaacaa gtatcattta gttgagtcgc tttggatttt
     2401 tccgacaatt attgaacagc ttggaataat acaaaaccta ttagaaagac ctgcattatg
     2461 cggtctattt ttcaagtatt atgatatttc agtttttcgg tttttcaata atttttaacc
     2521 aaaaaatgtc ggttcttcaa aatatcctaa tccgcacaat acaacctgta tccgcactct
     2581 gcgcaagcct tttcatcctt cgtcctctca aacccatgtt catagtcata cgcgatcgac
     2641 tttgcgatct caatcatacc atcaatagca ttctcatcaa gcccctccat ccgtgagctt
     2701 ccagcgcact catactttcc accatcaagt tcaaatgatc tgggatttgg gatattaagc
     2761 aattccagtg tgagtctttt cactttatat tgaggataaa catgctcgat ccccctggca
     2821 taaagcaaaa gttgttttga acgctcatca ggtctgggtt cagattttcc ggtcttgtaa
     2881 tcgatgatct cgatttcgtt cttcgttccc gatatgagat ccactcgatc aataaatcct
     2941 ttgaaattaa atcctccaag cggtactgta aaatgctgct caaccatcag gttattcgta
     3001 attttatcct tattgcgctc ccagaataca tccaatgctc cagttgcagt ttcgatatca
     3061 acgcccctga aatcaggttc ttttgcgacc atgtccctga tctcatacag cttatctttt
     3121 gagaatattt tctcactcac cgcagtttcc agtacactat gaacaaaatt cccacgtctc
     3181 atggctccgg tagaatcttc tgaatcgcgt gtaggcatcc tgagaagttc cttgagctca
     3241 tactgccggg gacatttctc ataagtactt attgaggaat agctaagagt catgcttgca
     3301 ggattgaatt tcaacccgtt tttattatct tttattttgc tgagaatctt ttcagcgtca
     3361 tctgcaggat ttatttttgg ccagttggaa ttgaaaatct ccttaaactc catcgctttt
     3421 ccaagcttta aagcctgata cagcagcgta ttcttcatga tctcattaaa atctccggaa
     3481 tcaagcgatt cgatgataag cctcttacgc attgcttttt cccgctcaag cgcactatcc
     3541 tttaccattt ccctgacttt tgtatcagta tccctgaaat aactgagatc tccaaccgtg
     3601 atattcccac cagccctcca gttgtcatat ccaagatcct gaaggaactc tgatggctcc
     3661 ctttcatctt caccataatt cactgccagg gtaaggaaaa gatgttcttt ggctcttgtg
     3721 aacgccacat acgcaagccg tctttcttct tcctttttga tctctttttt gcgttctctg
     3781 actgcttttt cagtatcttt aatggaaccg tctgcaaaga tatccatgta ctggtccatc
     3841 aactcaggcg gaataagtgg ttctgtgcct ccccggtaca gtggaaattt atcttttgca
     3901 aggtttgtga gaaatacaat attgaactca agcccctttg ccgcatgtat ggtcatgaga
     3961 tttacggcat cctcatcagt tattttcgca gaaggcgggt ttccgtccat ctcgtcaagt
     4021 atctcaagat aatcaatgaa caaactcagg tcttttccgt ggaactcttc aaaattgctt
     4081 gtcaattcat gaagttttct gagattcaaa agcgcttctc tactgcgctt tgtgtctgta
     4141 tgagagaatt gccttgagag acctgataga tcatatactt caaggatcag gtctgataca
     4201 tccagattct tcttcccgca cagcgaatcc attctctttt tcacattgtt tatggtctca
     4261 agcccgctct ttgaaagtcc aagttctcca atatggtgat aaatggcttc ctggaacgag
     4321 acccattttt tcttgagata ttctcctatc cttatggaat cttcctggct cagggcatta
     4381 ttgtaatgga atatcctcca ccatgcctct gt
//
LOCUS       DATGBN010000170         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_51788, whole genome shotgun sequence.
ACCESSION   DATGBN010000170 DATGBN010000000
VERSION     DATGBN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51788"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VKL21_04010"
     CDS             complement(<1..394)
                     /locus_tag="VKL21_04010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMB44968.1"
                     /translation="MRKVLILILAAVILFSGCVEKTVKKGDNISVNYIGSFENGKVFD
                     TSIESVAKANDLFTPGAAYEPFNFSVGKTPSETIEGFDEGVIGMKVGQTKNLIIPPEK
                     AYPIIPNKIQVLPIIDNIPTITVIPRDLK"
     gene            488..1516
                     /locus_tag="VKL21_04015"
     CDS             488..1516
                     /locus_tag="VKL21_04015"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HMB44969.1"
                     /translation="MEIPTLLLPLLTLSASLIIAVAISVIIKILQKKAEKTNTKIDDI
                     ILHTIGRPLYILVIVAGIYYAIHQTPVLADLIDRYDQGYKYRHFIVTIFLTWIVASLA
                     KSIIKEYGYDLAAKTEGEMDGRIVALADMSVTYIIWVFGLMLALAGVGIEITPLITGM
                     GIAGLAIALAAQNLLSNVFGGVTITIDQLYKVGDRVEMGGVYGDVYEIKPRYTMIKTL
                     NNTIVTIPNSKVINEQIINYAVPDETIRVKIPVNVAYGTDPKRVEKILLEIADKISLI
                     LKEPQATVRFTEFASSSQNLELIVWVRHYDEKHPVIDMILREMFIKFKNEGIEIPFNQ
                     MDVHIKKD"
     gene            complement(1779..2111)
                     /locus_tag="VKL21_04020"
     CDS             complement(1779..2111)
                     /locus_tag="VKL21_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44970.1"
                     /translation="MKMKRQIVTILVVLMAIIGLSGMTAAQSVGSKKKCEDGNNGIAA
                     TLTVTGGHSDTYNHPGDGKCNDNKDGEKDHKKDHKKDDNKDDEKDHKKGHKKDHKKDD
                     NNEEGQPL"
     gene            2383..2796
                     /locus_tag="VKL21_04025"
     CDS             2383..2796
                     /locus_tag="VKL21_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44971.1"
                     /translation="MELTRKTLLSCGCALVLAFSLFILLVLLTALPISYFSILVQKLI
                     LILIFIFSLIIAVPVILRKLNRLPNEKQILGWTFIGLGVEFILFPFMLLIYIFNFSSA
                     ISLIIGVSIVMLSLIFGLSAGLISIVMGISLVKRK"
     gene            2808..3149
                     /locus_tag="VKL21_04030"
     CDS             2808..3149
                     /locus_tag="VKL21_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMB44972.1"
                     /translation="MVEDDELAQIKKKRLEELMKRQHEALNPKPQVVIEVFTSPTCPH
                     CPRALAMAQELASQMPGVQAFEVSTANPQGFAKAALYGVKAVPTVFINRKRAFVGAPP
                     SIEVLRQALKS"
     gene            complement(3146..3607)
                     /locus_tag="VKL21_04035"
     CDS             complement(3146..3607)
                     /locus_tag="VKL21_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44973.1"
                     /translation="MIPLNTGEMLLVRGKEGTLGIVKVGENKQFFLETDTEEIILALE
                     PEDLLVASGFGTDETIIKGLKCILFMIREVGSPFIALSKKHPASKRLKIVVSAGDRTR
                     VSCSITPGTHPEQDILCGSKEFDGLEISGVKDGVEFKNLKDGNFMKIPFDI"
     gene            complement(3604..3765)
                     /locus_tag="VKL21_04040"
     CDS             complement(3604..3765)
                     /locus_tag="VKL21_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44974.1"
                     /translation="MCSVGDAFNIEIQQLNAKFYKCSDCSNEFRGVGKKVICPSCQSG
                     KVSLMFKEE"
     gene            3903..>4966
                     /gene="dnaJ"
                     /locus_tag="VKL21_04045"
     CDS             3903..>4966
                     /gene="dnaJ"
                     /locus_tag="VKL21_04045"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMB44975.1"
                     /translation="MATKRDYYEILGVEKKASADEVKSAYRKLAMQFHPDKNKSPDAE
                     EKFKEISEAYAVLSDQGKRQQYDQFGHAGIDQRYSQEDIFRGADFEDLFKDIGMGGRT
                     RGGGSIFDIFFGGGGGRHEGPGRGSDLLYELAINLEDAASGKAAELEVPRTEACDVCH
                     GSGAKPGTSPKTCSTCRGSGQVNRTQNTPFGRFMTTSTCTTCRGTGTIIDSPCNACHG
                     AGTVQRRRKIEVKIPPGVDTGSRLRVTGEGEAGSKGGPPGDLYVEVNIRPHNIFTRNE
                     NDLIMEATISFTQAALGDEIIVPTLDGKAEMKIPSGTQNGQVFRMKGKGIPGLHMSGK
                     GDQLVKVKVSVPTRLTDRQK"
BASE COUNT         1460 a         1005 c         1035 g         1466 t
ORIGIN      
        1 cttttaggtc cctgggaatt accgttatgg tgggaatatt atctattatc ggcaagacct
       61 ggatcttgtt aggaattatc ggataagctt tttccggagg gatgattaaa ttctttgttt
      121 gtcccacttt catcccgata accccttcgt caaatccttc tatagtttca cttggtgttt
      181 ttccgacact gaaattaaag ggttcgtatg cggcgccggg agtaaataaa tcattagctt
      241 tagccacact ttcaatagaa gtgtcaaaaa cttttccgtt ctcgaaactg ccaatataat
      301 ttacggaaat attatcccct ttttttacag ttttttccac acagccactg aataaaatta
      361 cagctgctaa tataagaatc aatacttttc tcatgatatc actttatcca gagtgatgat
      421 aacatataag taatttgtga ttatttaatg aatgtatttt ggactattta ttcatcagga
      481 acaagacatg gaaattccca ccctactcct accattactc acattgagtg ccagtttgat
      541 catagctgtt gccataagcg tcattataaa gatactgcaa aaaaaagcgg aaaagacaaa
      601 tacaaagata gatgacatta ttctgcatac gattggaaga cccctatata ttcttgtgat
      661 agtggcaggg atctattatg caatacatca gaccccggtt cttgcagatt taatagatag
      721 atacgatcag ggatataagt acaggcactt cattgtcaca attttcctga catggatcgt
      781 tgcatccctg gcaaagagta ttataaagga atacgggtat gaccttgccg caaagacaga
      841 aggagaaatg gatggcagga ttgtggctct tgccgatatg tcggtaactt atattatctg
      901 ggtatttgga ctgatgcttg cattagccgg tgtagggatt gaaataacac ctcttatcac
      961 cggaatggga atagccggcc tggcgatcgc actggctgcc cagaacctgc tttcaaacgt
     1021 ttttggaggc gttaccatta ccattgacca gctttataaa gtaggggaca gggtagaaat
     1081 gggcggagtc tatggggatg tatacgaaat aaaacccagg tataccatga taaaaacatt
     1141 gaataatacc attgtcacga ttcccaattc caaggtaatt aacgagcaga tcataaatta
     1201 tgcagtgcct gatgaaacga taagagtaaa gatacccgtt aatgtagcgt atggcacaga
     1261 tccgaaacga gtggagaaaa tcctgcttga gatagcagat aagatatccc tgatcctgaa
     1321 agaacctcag gcgactgtgc gttttacaga atttgcttct tcctcgcaga acctggaact
     1381 gatcgtctgg gtgaggcatt atgatgaaaa gcatcctgtt attgacatga tattaaggga
     1441 gatgttcata aagttcaaaa atgaaggcat agagatacct tttaaccaga tggatgtcca
     1501 tattaagaag gattaatatt aatgtcgaag gcgggcaaag ccaatttcag tgcatatgat
     1561 atcagaggcc tgatcgctgg cagcggtcta tccgtgggac agaacgaaaa tgtgaaatga
     1621 taattataaa ttattttatt gtatgctaaa ataagaattt gcatagaggt ttactcgttg
     1681 agggaaaaat cccgataagg ggtctcgtcc tcaactcgca tttttcttaa cagaattgaa
     1741 aggttacctg gaacagttac ttattttttc caaactaatt acagtggctg cccttcttca
     1801 ttgttatcgt cttttttatg gtctttttta tggccttttt tatggtcttt ctcgtcgtct
     1861 ttgttatcgt ctttcttatg gtctttttta tggtctttct cgccgtcttt gttatcgttg
     1921 cacttgccgt cgcccgggtg gttataagtg tccgaatgac caccggtaac tgtaagtgtt
     1981 gctgctatac cattatttcc atcctcgcac ttttttttgc ttccaacact ttgtgctgcc
     2041 gtcattcctg ataaccctat tattgccatt aagactacca aaattgttac aatttgtctt
     2101 ttcattttca tatttcaaac tccttttcgt gtttctcaag tatatataat agttctctga
     2161 caattccaaa accaccaata cctgatgtta tccgcattat ctatttgcat ataaatcttt
     2221 ccaatgaaat ttatcattat gtaggattag tttcataatc ttatttgcta atatagtcac
     2281 atgatcgatg aaaaaatcca tatgtgatga atcggagctt gttgtcataa ttaaaacaac
     2341 tgcaaaaaat atgaacaaaa tcaaatatta aaaaagattg aaatggaatt aacacgaaaa
     2401 acattacttt cctgcggatg cgcacttgtt ctggcattca gccttttcat tcttcttgtt
     2461 cttctaacag ccctgccaat atcctatttt tcaattttgg ttcaaaagct tatcttgatc
     2521 ttgatcttta ttttctcatt aattattgca gtacctgtta ttttacgaaa actgaaccga
     2581 ctcccgaatg agaaacagat actgggctgg acttttatcg gacttggcgt ggaatttatc
     2641 ctatttccgt tcatgctatt gatctacatc tttaattttt catctgcaat cagcctgatc
     2701 attggagtat ctattgttat gctttctttg attttcgggt tatcagcggg tttaattagt
     2761 atcgtaatgg gaatttcatt ggttaaaaga aaataacgag gaataacatg gtagaagacg
     2821 atgagcttgc acagataaaa aagaagagac tcgaagaatt gatgaaacgc cagcatgaag
     2881 ctttaaatcc aaaaccccag gtcgtaatag aagtattcac ttccccaaca tgtccacact
     2941 gccccagggc gcttgcaatg gcgcaagaac tcgcttcgca gatgccaggc gtgcaggcgt
     3001 ttgaagtttc aacagctaat ccgcagggat ttgccaaagc tgcgttatat ggtgttaagg
     3061 ctgttccaac agtgttcatt aaccgcaaac gcgcttttgt cggtgcgccg ccatctatcg
     3121 aagtattgcg gcaggcattg aaaagttaaa tatcaaaagg gattttcata aaattcccat
     3181 ctttaagatt cttaaattcc actccgtctt ttacacctga tatctcaagg ccgtcaaact
     3241 cctttgatcc gcataagata tcctgttctg gatgcgttcc cggcgttatg ctgcagctta
     3301 ccctcgtccg gtcgcctgca gaaacaacga tcttgaggcg ttttgaagcg ggatgctttt
     3361 tcgaaagggc aataaaaggt gagccaactt cgcgtatcat gaaaagaatg catttcaaac
     3421 cttttattat ggtttcatca gtcccgaaac ccgatgctac aagcaaatcc tcaggttcaa
     3481 gggcaagtat tatttcctct gtatccgttt ccaggaagaa ttgtttattt tctcctactt
     3541 tgactattcc aagagttcct tcctttcccc gcaccagaag catttcgccg gtattaagcg
     3601 gaatcattct tccttgaaca taaggctcac tttgcctgac tggcatgaag ggcagatcac
     3661 ttttttcccg acacctctga attcattact gcaatcgctg cacttataga attttgcgtt
     3721 taactgctgt atctcaatat tgaatgcatc accgacactg cacatgcttt ttcacctcag
     3781 ttttgattgt gcatgattaa tcctattctt atattaactt tatgcaagat acaataaacc
     3841 acaatctata ttttaattga aagacattag cacaaaaaaa tagacgatgg ttcattaatt
     3901 ttatggctac taaacgtgac tattatgaaa ttcttggcgt ggagaaaaaa gcgtcagcag
     3961 acgaagtaaa atcagcatat cgtaaattag ctatgcaatt ccatccggac aagaacaaat
     4021 cacccgacgc cgaggagaaa ttcaaggaaa tttcagaagc ttatgcagtg ttatctgacc
     4081 agggtaaaag gcaacagtat gaccagttcg ggcacgctgg tattgatcag aggtattcac
     4141 aggaggatat tttcagggga gctgatttcg aagacctttt caaggatatc gggatgggtg
     4201 gacgcacaag gggtggggga agcatatttg acatcttttt cgggggcggg gggggaagac
     4261 atgaaggtcc aggcaggggt tcagatctac tttacgaatt agccataaat ctggaagatg
     4321 cggccagcgg gaaagcagca gaattagaag tcccaagaac agaagcatgt gatgtatgtc
     4381 atggaagcgg ggcaaaacca ggaacatctc ccaagacatg ttcaacctgc cgcggcagcg
     4441 gccaggtcaa ccggacgcag aatacgccat ttgggaggtt catgacaaca tcaacttgca
     4501 caacctgccg gggaactggc actattatcg attctccatg caatgcatgc catggcgcag
     4561 gtactgtcca gagaagacgt aagatagaag tgaaaatccc gccgggagtt gatacaggct
     4621 caaggctgcg cgttacgggt gagggcgaag caggctccaa aggcggaccg cccggagatc
     4681 tttatgtgga agttaatatc aggccccata atatttttac acgtaatgag aatgacctta
     4741 tcatggaagc aactatcagt ttcacccagg ctgccctggg tgatgaaata atagttccaa
     4801 ctctagacgg gaaggctgaa atgaagatcc catcagggac gcagaacggc caggtgttcc
     4861 gaatgaaagg caaaggaata cccggacttc acatgtcagg gaaaggcgac cagcttgtga
     4921 aagtcaaggt ttcagtcccc acaagattga ctgacaggca aaaaga
//
LOCUS       DATGBN010000171         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_323850, whole genome shotgun sequence.
ACCESSION   DATGBN010000171 DATGBN010000000
VERSION     DATGBN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323850"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..668
                     /gene="rnhB"
                     /locus_tag="VKL21_04050"
     CDS             9..668
                     /gene="rnhB"
                     /locus_tag="VKL21_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMB44976.1"
                     /translation="MISGIDEAGKGPVLGPMCIAGILLDGTKTDELLRIKVKDSKQLT
                     GKKREVLAGEIKKIVQEYFILEVSPSRIDELRKIMTMNEIMVICFTKVLEELRPDHAF
                     VDAADVIANRFGENIKERYSGEILITSEHKADVKYPIVSAASILAKVQRDSLIKQLED
                     ELGMEIGSGYPADPKTIRFLENWVKNNDSLPSFARYSWETSKRILEKYDRNQKTLFQY
                     E"
     gene            complement(706..1251)
                     /locus_tag="VKL21_04055"
     CDS             complement(706..1251)
                     /locus_tag="VKL21_04055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMB44977.1"
                     /translation="MKRIAAISIIILISITLGCVSDNNSVNIEKNQSITSGNNRIATI
                     VTEKGTIKLELYEKETPITTKNFIELAQKGFYDGLTFHRVEPGFVIQGGDPKGDGTGG
                     SGKTIPLEINQTLTHKKGAVAMARSSDPDSASSQFYITLEDAKQLDNSYAIFGQVIAG
                     QDVVEKIKKGDKMLKVTVGEE"
     gene            complement(1267..1509)
                     /locus_tag="VKL21_04060"
     CDS             complement(1267..1509)
                     /locus_tag="VKL21_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44978.1"
                     /translation="MSLVNNIIKMFIGVVVLIIGIVWYTGMYGYLGYWNNLIDLLKAT
                     IGFFLLFVGLIVAWMGYDDYKMDREMEKEEKKEEPK"
     gene            1602..1675
                     /locus_tag="VKL21_04065"
     tRNA            1602..1675
                     /locus_tag="VKL21_04065"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1636..1638,aa:Val,seq:tac)
     gene            1679..2350
                     /locus_tag="VKL21_04070"
     CDS             1679..2350
                     /locus_tag="VKL21_04070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB44979.1"
                     /translation="MIPTKCILCDNSSVLQLTFTKTYKKLGKRIYFRCPECDLIFVPE
                     RFHLSPGDEEARYMLHHNTISNEGYVKMFREKISLVRQYCPGISSVLDYGCGNEPVLT
                     ELLKREEYDCDRYDLYFHPEFPEGSYDLVISTEVFEHFRDVRSEINKISSLINPGGFL
                     AVMTSFHDPIDHFEEWWYHSDPTHICFFSLRTFDLISKQFGFEVIYTNRKNFIIFKKC
                     KNTNI"
BASE COUNT          769 a          481 c          469 g          766 t
ORIGIN      
        1 tggtttttat gatttccggg atcgatgaag caggaaaagg tccggtactt gggcccatgt
       61 gcattgcagg aattctgctt gatggaacaa aaacggatga gcttttaagg atcaaagtga
      121 aagactcgaa acaattgact ggaaaaaaac gtgaagtact ggccggggaa attaagaaaa
      181 tagttcaaga atattttatt cttgaagtta gcccatccag gatagatgaa ctccgaaaga
      241 taatgacaat gaatgaaata atggtcatct gctttacgaa agtccttgag gaattaaggc
      301 cggatcatgc ttttgtggat gcggcggatg taattgcgaa cagatttggc gagaatatca
      361 aggagagata ttcaggggag atattgatca cttcggaaca taaagcggat gtgaaatacc
      421 cgatcgtatc ggctgcttct atccttgcaa aagtacagag ggattcactt ataaaacaac
      481 tggaagatga actgggaatg gaaataggaa gcggttatcc agctgacccg aaaacaatca
      541 gatttcttga aaattgggta aagaacaatg attctctccc ttcttttgcc cggtattcct
      601 gggagacttc aaaacgaata ttggaaaaat atgatagaaa ccagaaaacg ttgtttcaat
      661 atgaatgaag agagaaatat ttcgataaaa gaggatagaa aagtcttatt cttccccgac
      721 cgtcactttt agcatcttat cccctttttt gattttttcc acaacatcct ggcctgctat
      781 gacctgtccg aatattgcat agctattatc aagttgcttt gcatcttcaa gagtaatata
      841 gaactgtgag ctggcgctat cagggtcact ggatcgggcc atggctactg cgcctttctt
      901 atgtgtcaat gtttgattta tttcaagagg aatggtcttt cccgaacctc cggtgccatc
      961 tcctttaggg tcgccgccct ggatcacaaa tcccggctca accctgtgga atgtcaaccc
     1021 gtcatagaat cctttttgtg ccagctcaat aaaatttttt gttgttatag gcgtttcttt
     1081 ttcatataac tctaacttga tcgtaccttt ctcggtaacg atagttgcta tcctgttatt
     1141 cccggatgtg atggattggt ttttttcaat attcactgaa ttattgtcac ttacgcatcc
     1201 caatgtaatt gatatcaaaa taatgataga tattgctgcg atacgtttca taccgggaat
     1261 tataagttac tttggctctt ctttcttctc ttctttttcc atttccctgt ccatcttgta
     1321 atcgtcatag cccatccatg ctacgatcaa gcccacaaag agaaggaaga aaccgatagt
     1381 tgctttcaat agatcaataa ggttattcca gtatccgaga tatccataca tacctgtata
     1441 ccacacaatc cctattatca gaacaacaac gccaataaac atctttataa tattatttac
     1501 aagagacata ttttcaccat gttaaattgt tttatcttat attaaagttt ttttatgaac
     1561 taaataacta tataccccaa acaacatctt agaatcccgc agggctcgtg gtctagtcgg
     1621 ctatgacatc gcctttacac ggcggggatc cggagttcga atctccgcgg gcccatttat
     1681 gattcctaca aaatgtatcc tgtgtgataa ttcctctgtt cttcagttaa catttacaaa
     1741 aacttacaaa aaactgggaa aaagaattta tttccgctgt cctgaatgtg acctgatttt
     1801 tgtgcctgaa agattccatc tttcacccgg ggatgaagaa gcacgatata tgcttcatca
     1861 caatactatt tcaaacgaag gatatgtaaa aatgttcagg gaaaaaatat cccttgttcg
     1921 ccaatattgc cccggaatca gctcggtact tgattacggg tgtgggaatg agcctgtttt
     1981 aacagaatta ctcaaaagag aagaatatga ttgtgacaga tatgacctct atttccaccc
     2041 cgaatttcct gaaggctcat atgaccttgt tatctcaaca gaagtgtttg agcatttcag
     2101 ggatgtaaga tctgaaatca acaagatcag ttcactgata aatccagggg gattccttgc
     2161 agtcatgaca tctttccatg atccaataga tcattttgaa gagtggtggt accatagtga
     2221 tcctacgcac atttgttttt tcagcttgag aacatttgat ttgatttcaa aacaatttgg
     2281 atttgaggtc atctatacaa atcgaaaaaa ttttataatt tttaagaaat gcaaaaatac
     2341 aaatatttaa attataaata tttaatttga aaatacaatc agcttaatat tcgttgtacc
     2401 attaataata atacgaatca tgctcttcga acacttgata tactccacgg caatcgcgat
     2461 catagcaggc atgatccact tcaaa
//
LOCUS       DATGBN010000172         5005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_207442, whole genome shotgun sequence.
ACCESSION   DATGBN010000172 DATGBN010000000
VERSION     DATGBN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207442"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1029..1280)
                     /locus_tag="VKL21_04075"
     CDS             complement(1029..1280)
                     /locus_tag="VKL21_04075"
                     /inference="COORDINATES: protein motif:HMM:NF022282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HMB44980.1"
                     /translation="MKKIDDGVKNRLTKFLLKDKIGIRKSLLRLFSEAKSCNTVEIYD
                     YLIKQGFNVNYRAVSSMVGQMHSRIGILRVSLANERQSV"
     gene            complement(1355..1507)
                     /locus_tag="VKL21_04080"
     CDS             complement(1355..1507)
                     /locus_tag="VKL21_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44981.1"
                     /translation="MCSVGDAFNIDIAEGIAPRKYKCKDCAKQFKGIGTNPKCPECHS
                     ANTETT"
     gene            complement(1655..2419)
                     /locus_tag="VKL21_04085"
     CDS             complement(1655..2419)
                     /locus_tag="VKL21_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1670 domain-containing protein"
                     /protein_id="HMB44982.1"
                     /translation="MTSNIAGKSIGSAIKSLLTSEYKFMGGDKVQEMFVTDVMKLFKE
                     YNRDAWNLQPGQTMWFAVCADEKQSYGKTLEKTRITPVILSVVHDDDRKTRENGYSHK
                     ELRKFKIARILREAYEQKGVLSQADVAEMLGISTGTVSRDIREYQAENQVVLPYRGTI
                     HDLGRAITHKKMIIGHFLKNVPTPDISRITDHTEEACDRYIKAYKNVRNLYDRMDQNE
                     IARILEMSESLVKEDVAIHEEFSKKEEKINDGSSKE"
     gene            complement(2724..2885)
                     /locus_tag="VKL21_04090"
     CDS             complement(2724..2885)
                     /locus_tag="VKL21_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44983.1"
                     /translation="MNKDIQKLKTEIFDSVKNNMEEKDVVAIIERSVNMTLKYVDTQV
                     STHKFFKDD"
     gene            complement(3173..3352)
                     /locus_tag="VKL21_04095"
     CDS             complement(3173..3352)
                     /locus_tag="VKL21_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44984.1"
                     /translation="MDIFDVLSAISKRRTTLMDRGITAHKALLKAERVISKEYHIPLP
                     DIQKLVGNEIKPRSL"
     gene            complement(3433..4866)
                     /locus_tag="VKL21_04100"
     CDS             complement(3433..4866)
                     /locus_tag="VKL21_04100"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HMB44985.1"
                     /translation="MSRIETDSIGNVEVPDNVLYGAFTTRASRNFKISGIKADPHFIR
                     SLAIIKKACAKANVKLNMLNETIGDVIIQACDEVIEGRFSDQFILDVFQAGAGTPFNM
                     NMNEVIANVAILKLGGDPGDYTKVHPNNHVNMAQSSNDVIPSTIRLSVLLSLLPLKKE
                     LEELIVAFQSKAKEYDHVIKVGRTHLEDAVPIRYGQVFNGYAASLEKSMERLKTSEKS
                     MLELGIGGTAVGTGINTHPNFKKMVISELNLLTGFEFHAGNSIMLSWSMTGFVDISNS
                     LRMLAIEMNKISNDLRLLNSGPKTGISEIVLPEVEPGSSIMPGKINPSIAEAVNMVCF
                     QIAGNDHAVAMAAEAGQLELNVMTPLIAFDLLWSIELLTNTIRMFREDCLSGIIVDEK
                     RCNEFIENSHTFATVLNPYIGYDMVAQLVKTALSENKSLKQALIEKDIIPSKYLNDIL
                     DPGKMTEPGIVDRKIIEDIILFGKKNR"
BASE COUNT         1454 a         1012 c          839 g         1700 t
ORIGIN      
        1 tgctttaact aattttttga tgtcaaaaag tggtatgtga tattcattag aaacatcaaa
       61 ttctgctttc atcaaggctt cattttcatt gataccgcta cgcataaatg tttttttctt
      121 ctttgagata gcagttaata catcgaatat gtccatttgt aatcgcctcg ttatcttgcc
      181 gtaattgaat tactaccctc acatctcctg tttttctaaa tgcaaaaaag gatgaaaatc
      241 gaataattca ttatgataat tgtgttgatt atgattaaat agttatcgca attgataatg
      301 atgacaagtt ttgttgaagc actaacatcg taatataaaa agctcataga tatttgccgg
      361 aaatttatgt attcagtatt caaaatatta aaatgcgggg aaaaaggtta actattgtaa
      421 gtatattatg attattagta ggagtataaa cagatgcatg ttagaataat gcactgcata
      481 aaatgtttgg ggtataaaaa tgcggacgag aaatcggcgt aggcaaagaa aaagaaaagg
      541 gcgtatgcct ctctttccct aatcacagaa gatattgctt tttggtcgca gggatgttat
      601 gtggtgaaaa tgaatttatt ataatactat atatttccat tggataaata aggttaattg
      661 atggaataga ataggcagtg atgcctacct tataaataaa acctaatttt tccctaattc
      721 cctcaatcgc tcaatacacg cctgcgcctc atcaaccgct tttctctgct gtttttcaag
      781 ttgctttcaa gttgctttca agttgatgca aacccattat agttgtttgc caatcacaga
      841 gagattgaat cctacttgaa aaaggcgtca aaattatctt tacaatgtca aaatagtctt
      901 ctttgagagt ataaacatta cgtcagagtg tgtaaaaagt cgaaaaaaat cagtgttgct
      961 tgaaaaataa gccatattaa gcctttaaat ctgaaaataa gctttccatt ttgcaaaatt
     1021 atgagttatt acacactctg acgttcatta gccaaagaga cccgtagaat cccaattctg
     1081 ctatgcatct gtccaaccat agaagagact gctctataat taacgttaaa accctgcttt
     1141 atgaggtaat cataaatttc aacggtatta cagctcttcg cctctgagaa taaccttaga
     1201 agggatttcc taatacctat cttatctttc aggagaaatt ttgttagtct atttttaacg
     1261 ccatcgtcta ttttcttcat tgaatcacct tttcgacata tcataattga gcagataaaa
     1321 actttaattg gataggagga gctaagaata ttcattatgt agtttcggta tttgcagaat
     1381 ggcactctgg acattttgga ttagtgccta tccccttaaa ttgtttcgca caatctttgc
     1441 acttatattt gcgtggcgct atgccttcgg caatatctat attaaaagcg tctccaactg
     1501 aacacataat ttcacctccg tatttatttt ttaactctta taatctatta attattttct
     1561 ttatcttaac tcgtgataaa gaacacccat atgatatatg taatttttct aatagaaaaa
     1621 aaattgtaaa agtacctaaa tgctattatc ccgattattc cttacttgaa ccgtcattaa
     1681 ttttttcctc cttcttgcta aattcttcat gtattgcaac atcttcctta accagtgatt
     1741 cactcatttc cagaattctg gcaatttcat tctgatccat cctatcatag aggtttctta
     1801 catttttata tgcttttata tatcgatcgc acgcttcctc agtatgatct gtaattcttg
     1861 aaatatcagg agtggggaca ttcttcagaa aatgcccaat gatcatcttt ttatgagtta
     1921 tagctctccc aagatcatga atcgttccgc gatagggcaa aacaacttga ttctctgcct
     1981 gatattctct gatatctctg ctgaccgttc cggtgctgat acccaacatt tctgccacat
     2041 ctgcctgtga tagaactccc ttctgttcat atgcttctct cagaattcga gcaatcttga
     2101 acttcctgag ttccttatga gaatagccgt tctctcttgt ttttctgtcg tcatcatgca
     2161 caaccgacaa aatcacagga gtaattctcg tcttttcaag cgtttttcca taactctgtt
     2221 tttcatcagc acatacagca aaccacattg tctgtcctgg ttgaagattc caggcatccc
     2281 ggttatattc cttgaaaagc ttcattacat cggtaacgaa catttcctga accttatctc
     2341 ctcccatgaa cttatattcc gatgtgagaa gtgatttgat ggcagagcct atgctttttc
     2401 ctgcaatatt tgaggtcacg ggatcatctt catattttat ttactctgct gtatccacat
     2461 acctttaaaa gcttcgatat tgaatccaac cgtgaaaaag gggggttgcg accatattaa
     2521 taacttcaca atccccacac gaatcgatgt ggtcgttatt atatcgtttt caggcggctc
     2581 ttcctgccct tgaataattt atccccaaca ggtattacaa tggtctgtgg caggtacata
     2641 actttgacct gcaaatgcaa ggcaatgtgt acaatattat gactatttga cttactttac
     2701 atcgccaaag cgggtattac aacttaatcg tccttgaaga atttatgtgt ggaaacttgc
     2761 gtatcaacat attttaaagt catatttacg cttctttcta taatagccac tacgtccttt
     2821 tcttccatat tgttcttcac actatcaaat atttctgttt ttagtttttg tatatcttta
     2881 ttcattttaa cctcttttct atcccctata atatagaatc gcttaacgtc ggctttgatg
     2941 atataatctc ccatcttgaa aggcaacaga gaaaagtggt agaagatgca catgctaaga
     3001 ttgaaaggtt gaaggaatta gagaaataaa tgatattttc cgaatgtgtc atttttctaa
     3061 atatgtatgg tctatacaca acgattttta agcaagatag tgctcacacg ttcaaaccat
     3121 ataattattc gcatcaaaga ttccccccgg atcttataat gccatgaaat ctctatagac
     3181 ttctaggttt gatttcattc ccgacaagtt tctgtatgtc aggcaatggt atgtgatatt
     3241 cttttgatat aaccctctct gctttaagta gggctttatg tgccgttata cctcgatcca
     3301 tcaaggtggt tcttcttttt gaaatggctg ataaaacgtc gaatatgtcc attagttcag
     3361 cctccttaaa acaatattct aattcaatgg acttaaagtt atttgaaata attaataatt
     3421 ataattaaag attcatcgat tcttttttcc gaataaaata atatcctcga taatttttct
     3481 atccacgatc ccgggctctg tcatttttcc gggatcaagg atatcattca aatatttcga
     3541 tgggataata tccttttcaa taagcgcctg cttcagtgat ttattttcag aaagagcagt
     3601 tttaactaat tgtgccacca tatcataacc gatatacgga ttcaacactg ttgcaaatgt
     3661 atggctattt tcaatgaact cattgcatct tttttcatcc acgataattc ctgataagca
     3721 atcttcacgg aacatcctta ttgtattcgt caggagctcg atactccaca agaggtcaaa
     3781 tgcgataagc ggtgtcatca cattaagctc aagctggcct gcttccgcgg ccatggcaac
     3841 agcatggtca ttacccgcga tctggaaaca aaccatattc accgcttcgg caattgaggg
     3901 attgatctta cctggcatta tggaagagcc cggttccacc tctggcagga caatttccga
     3961 aattccggtc ttaggcccgg aattaagaag acgcagatcg tttgagatct tattcatttc
     4021 aatagcaagc atcctcagtg aattggatat atccacaaaa cccgtcatac tccatgatag
     4081 catgatgctg ttgccggcat ggaattcaaa accagtaagc agattcaact ccgaaatgac
     4141 cattttctta aaattcggat gtgtattaat accggttccg actgctgtgc cgccaatacc
     4201 gagttcaagc atactttttt cggaagtttt cagtcgttcc atgctttttt caagagatgc
     4261 tgcatatcca ttgaatacct gcccgtatct aatgggcacg gcatcctcaa gatgagtcct
     4321 tccgactttg atcacatggt cgtattcctt tgcttttgac tgaaatgcaa ctattaattc
     4381 ttccagttcc ttcttaaggg gcaaaagcga aagaaggacg cttaacctta ttgtggaagg
     4441 tataacgtca tttgatgatt gcgccatgtt aacatggttg ttgggatgga cttttgtgta
     4501 atcgcccggg tcgccaccca atttcaatat cgccacattt gcgataactt cattcatatt
     4561 catattaaaa ggagtgcctg cgcctgcctg gaacacatca aggatgaact ggtcgctaaa
     4621 tctcccttcg atgacctcat cgcatgcctg gattatcaca tccccgatgg tttcatttag
     4681 catgttaagc ttgacattgg cttttgcgca tgctttcttg atgatggcga gggatcttat
     4741 aaaatgaggg tcggctttga taccgctgat cttgaaattc cttgaagcgc gcgtagtaaa
     4801 agcgccatat aacacattat cgggaacttc gacatttcct atactatccg tctctattct
     4861 gctcattttg actcatttag tgttgcactg tataattgtt gttcatttaa aatctattgt
     4921 atcaccataa ttctttagcc ttttgatgca atatgatacc attccattat gtccatagat
     4981 aaaatcataa taaaaggcgc acgcg
//
LOCUS       DATGBN010000173         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_440668, whole genome shotgun sequence.
ACCESSION   DATGBN010000173 DATGBN010000000
VERSION     DATGBN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440668"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1148)
                     /gene="cfbD"
                     /locus_tag="VKL21_04105"
     CDS             complement(27..1148)
                     /gene="cfbD"
                     /locus_tag="VKL21_04105"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HMB44986.1"
                     /translation="MPVKKEKEPLIMHPRPSSIVAALYTLRDLDTDVVILHGPPGCSF
                     KHARLLEEDGMRVVTTSLDESGFVFGGHDALVEVLEKVIERFNPRRIGIVGTCASMII
                     GEELHEAVTQVQPDVPVIEVEVHAGYRDNTKGVVMALESALACGIISEAEFERQKALL
                     KEATMVEKRHGAASKEYLEPNRGDLKYKVAGRLLELIKQGKKGLNILNAKKETAFMFA
                     DINLAVAEVAEKLKHVSNIVNMVNLDDNVGLPKNRRNAREIKDDLTEKGFKIHHIIGG
                     LDEYPVAGNTANRIISEKYKDYDFAVISGVPHALPMDNIKNMELFSITNGPRQVVPLK
                     EMGHTHVVVEIDLHPKTLGVCHIVESEFGATLRELAKDA"
     gene            1417..1650
                     /locus_tag="VKL21_04110"
     CDS             1417..1650
                     /locus_tag="VKL21_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44987.1"
                     /translation="MKIDGVHDKELTLWFLTKGCTLDEANCFADETIREIEFYKEYEA
                     YQDERLGNSRFGQKVEVSKRFIRATNEGAAIIS"
     gene            complement(1673..>2334)
                     /locus_tag="VKL21_04115"
     CDS             complement(1673..>2334)
                     /locus_tag="VKL21_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HMB44988.1"
                     /translation="VDNLFAARIDQKKALEEYKERIMEVVKDKDEDTRRSVEEDLNSP
                     CAEEMAAFEKFINYFELKGYDIIIFDTAPTGHTLRLLELPSDWKGFIDIGDLTKKTSD
                     ETKNKYANVIETMKSKEKSTFVFVMYPEYTPIIEAWRASEELKKQVGIETAMVAVNFL
                     LSQDYRKNRFFNNRRKQQEKYLFEIEQRFNTPILTVPLLEHEPEGLDKLRELGRKIFS
                     G"
BASE COUNT          599 a          541 c          433 g          761 t
ORIGIN      
        1 ctctttttcc gtaaatcgct atctgtttag gcatctttgg ccaactcccg cagtgtcgcc
       61 ccgaattcag attccactat gtgacatact cccagcgtct tgggatgcag gtctatttcc
      121 acaaccacat gggtatgacc catctccttt agaggcacta cctggcgcgg cccgttcgtt
      181 attgaaaata attccatatt ctttatgttg tccattggaa gcgcatgagg aacccctgat
      241 atgaccgcaa aatcataatc cttatacttt tcactgatta tccggtttgc agtatttcct
      301 gccacaggat attcatccaa tcctccgata atatgatgga tcttgaatcc tttttctgtt
      361 agatcgtctt tgatttcccg ggcatttctc ctgttcttgg gaagtcccac attatcatca
      421 agatttacca tattcacgat atttgaaaca tgtttgagtt tttcagcaac ttctgccact
      481 gcaaggttaa tatctgcgaa catgaatgca gtttctttct tggcattgag aatattgagt
      541 cctttcttac cctgctttat caattcgagc aatctccctg ctactttgta tttgagatcg
      601 ccccggttgg gctcaagata ttccttgctg gccgcgccgt ggcgcttctc caccattgtg
      661 gcttctttta agagggcttt ttgtctctcg aattccgctt cgctgataat cccgcaagca
      721 agcgcggatt caagcgccat gacaactcct tttgtgttat ccctgtatcc tgcatgcacc
      781 tccacttcaa taacaggcac atcaggctgt acctgtgtga ccgcctcatg caattcctct
      841 cctattatca tgcttgcaca tgtgcccacg atccctatgc gcctgggatt aaagcgctct
      901 atcacttttt caagcacctc cacaagggca tcgtgccccc cgaaaacaaa tcccgattca
      961 tcaagcgagg tcgttacaac tctcatgccg tcctcttcaa gaagccgggc atgtttgaag
     1021 ctgcaccctg gaggaccatg aagtataacg acatccgtat caagatccct aagcgtgtac
     1081 agggcggcta cgatcgaact tggccgcggg tgcattataa gtggttcttt ttcttttttt
     1141 acaggcatcc tttttctcca attgatttga gaacaaaatt atcttattca ttaaaaatct
     1201 tatctatagg atttccctat atagtcaatt attcaacctc aatatatgat acttattatg
     1261 aagttcttat tgctcacatc tgccagagca aatattggaa agatttcctg aaaatgatga
     1321 aacctgtcca ccgcttacga aagcattaaa tatttataaa cactaataat gatatgaggg
     1381 gtatttagtt agtatttgaa agggaggacg ataactatga aaatagatgg ggttcatgat
     1441 aaagaactga ctttgtggtt ccttacaaaa ggatgcactt tagatgaagc taattgtttc
     1501 gcagatgaaa cgattcgtga aattgaattt tacaaagaat atgaggcgta tcaggatgaa
     1561 cgattgggaa attcaagatt tggtcaaaaa gtagaggttt ccaaacgttt catcagagcc
     1621 acgaatgaag gggctgctat tataagctaa gattgcgtcc gatccaggca tatcagccac
     1681 taaagatctt tcttccaagc tccctgagtt tatccagacc ttcaggctca tgttcaagaa
     1741 gagggaccgt gagtattggg gtattgaatc tctgctctat ctcgaacaag tacttctctt
     1801 gctgttttct tctattatta aagaatctgt tttttctata atcctgtgaa agtaagaaat
     1861 taactgcgac catggcggtt tcaatgccaa cctgcttttt caattcttca gacgccctcc
     1921 aagcttctat tataggtgtg tattcagggt acatcacaaa aacgaatgtg cttttttcct
     1981 tgctcttcat tgtttctatg acattcgcgt acttattctt tgtttcgtca gaggttttct
     2041 ttgttaaatc ccctatgtcg ataaatcctt tccagtcaga gggaagttct aataatcgca
     2101 gggtatgtcc tgtgggagct gtatcaaaaa tgattatgtc atagcctttc aattcaaaat
     2161 aatttataaa cttctcaaat gctgccatct cttcggcaca gggagagttc aggtcttctt
     2221 ccacggacct ccttgtatct tcatccttat cctttactac ctccatgatc cgctctttat
     2281 attcttccag ggctttcttc tggtctattc ttgcagcaaa taaattatct acac
//
LOCUS       DATGBN010000174         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_168905, whole genome shotgun sequence.
ACCESSION   DATGBN010000174 DATGBN010000000
VERSION     DATGBN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168905"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VKL21_04120"
     CDS             complement(<1..223)
                     /locus_tag="VKL21_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44989.1"
                     /translation="MNKNEIIKATLKATKAKRKTQMCRVYETKIDLSHLNLITKEHLY
                     RLFLEAKWFYNYILSQHKIFGMDDKVHEVP"
     gene            397..753
                     /locus_tag="VKL21_04125"
     CDS             397..753
                     /locus_tag="VKL21_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44990.1"
                     /translation="MNAIHIAILIVGYIVLVGTSGKLLNYILTNFSARPVSQTLSKEA
                     IDTGFIIGKCENFLILTFMFLDAYTALALIFAAKSIVRREDMSKNSLFFLAGTMINVT
                     YSIMMGLAVKIVIGIS"
     gene            799..2547
                     /locus_tag="VKL21_04130"
     CDS             799..2547
                     /locus_tag="VKL21_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMB44991.1"
                     /translation="MIKNRLTWKIGGKAGEGIMATGMIFSQSCSCGGLHIYDINEYPS
                     LIKGGHNTLQVRVEDREIFSQVKGVNILIALNKETILLHKDELVDGGGIIYDSSEVID
                     DKEMRKNIRFYPIPLNDIIKEIGAQAIMKNNVALGASIALIDYDFGIVGEVIRDNFKR
                     KGDKIIDENTRSAKAGYDFIKKNFKNDFEYGLEKTGKPGRMLLTGNDAICMGAIRAGC
                     KFYSAYPMTPASSILHFMAAQERRFNIVVKHTEDEISAINMAIGAGFTGARAMCGTSG
                     GGFCLMSEGYGLAGMLEVPIVIVMSMRPGPSTGMPTWSEQGDLKFVLNASQGDFPRPV
                     LVPGDIEESFYLTGEAFNIAEKYQTPVIVLSDKNLAESHKTTEKFDEKKIAIDRGSFA
                     SPDDLAASSDTTFFKRYKYTENGISPRTIPGMKKGIYTGASDEHDEEGNIREDADIRI
                     KMMQKRGRKMMALSKELKQPELAGPNGADITIIAAGSTKGPIKEAMILLEKEGFKVNY
                     LQIVYLNPFPSEKVAKVIESAKKTVVVENNFSGQLADIIKEKTGRDVNKRVLKYDGRP
                     FYPEEIVTAIKEVAHG"
     gene            2540..3397
                     /locus_tag="VKL21_04135"
     CDS             2540..3397
                     /locus_tag="VKL21_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMB44992.1"
                     /translation="MVNMTVLNTIDKPNWCPGCGDFGILVALKGAVVELNIEQENIVL
                     VSGIGCSGKLPHWIRTYGLHGIHGRALPVASAVKLANNELTVFAVGGDGDGYGIGMCH
                     FIHAMRRNIDMTYIVHNNMIYGLTTGQTSPTSEKGMRTKSTPSGVIEVPVNPIALAIS
                     TGATFVARSFAGDVKHLQKTIVDAVKHRGFSLVDVFQQCVSFNHVNTYDWYKQRVYDL
                     STAGHDPADKLKAFTKSQEWGDHIPVGVFYKVLQPTYEDELPQIKDKPLVKQPIDNID
                     IGGLMDDFV"
     gene            complement(3398..>3993)
                     /locus_tag="VKL21_04140"
     CDS             complement(3398..>3993)
                     /locus_tag="VKL21_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HMB44993.1"
                     /translation="ARAAFGKKFEVAIGLETSNDLIRKYCINKGFSFSDFTLASQNAK
                     NNDVTVKAYLMQKPPFLSEGIAMNDMINSINDAAPFARTISMNLCNVQKGTLVDEMFE
                     RGDYRPPWLWSAVEALKAGKELSPGTVIMSDPVGAGSMRGPHNCGKCDKDVAEAIRIF
                     SVTQDTSVFKNLDCECKELWKKVIELEDLTFGAPLVR"
BASE COUNT         1168 a          795 c          943 g         1087 t
ORIGIN      
        1 caggaacttc atgaacttta tcatccattc caaaaatttt atgttgagat agaatataat
       61 tatagaacca ttttgcttca aggaataatc gatacaggtg ttcctttgtt atgagattga
      121 gatgagataa gtctattttg gtttcataaa ctctgcacat ttgcgtctta cgctttgctt
      181 tagtagcttt cagagtggct ttgataattt cattcttatt catgtgcaac ctttaacaag
      241 ttttatatgg ttttgatact atttattctt tgcctatctc caaaatgcag cctttaacat
      301 ctgtttttcg ggataggtat cacaattgca aagggtcgat tcatcccatc gctgaagctc
      361 tgggctttct cttacccttt gaacccatac cgtaaaatga acgcaatcca tatcgctatt
      421 ctaatcgttg gatatattgt acttgtggga acgagtggca aattgctgaa ttacattctc
      481 accaattttt cagcaaggcc tgttagccag actctaagta aagaggcaat agatactggt
      541 tttattatag gaaaatgtga gaactttctg atactcacct ttatgttcct ggatgcatat
      601 accgcactgg cactgatatt cgcagccaaa tccatagtac gcagggagga tatgagcaaa
      661 aactcgttgt tcttcctggc tggcacaatg atcaacgtta catattccat aatgatgggg
      721 cttgccgtga agatcgttat aggaatatca taaactatat tgcttatcga aaacaatctt
      781 ttctcaggag ctggtcggat gattaagaac aggttaacat ggaaaatcgg cggaaaagcc
      841 ggtgaaggaa taatggcgac tggtatgatc ttttcgcaat catgctcctg cggcggcctt
      901 catatatatg acataaacga atatccgtca cttataaagg gcgggcataa caccctgcag
      961 gttcgagtag aggaccggga aattttttcc caggtcaagg gcgtgaacat attaattgcc
     1021 ctgaataaag aaacgatatt attgcataag gatgaactgg ttgatggcgg gggtattatt
     1081 tatgacagct ccgaagtcat tgatgataag gaaatgagaa aaaacataag gttctatccc
     1141 atccctttaa atgatattat caaggagatc ggggcgcagg cgataatgaa gaacaacgtt
     1201 gctctcggag catcgatcgc tcttatcgat tatgattttg ggatcgttgg ggaagttata
     1261 agggataact tcaaaagaaa aggtgataag atcatagatg aaaatacaag atcagcaaaa
     1321 gccggttatg attttattaa aaagaatttc aagaatgatt ttgaatacgg gcttgaaaag
     1381 accgggaaac ccgggcgtat gcttctcaca ggcaacgatg ccatttgcat gggcgccatc
     1441 agggcaggat gcaaattcta ttccgcttat ccaatgaccc ccgcctcttc tatattgcat
     1501 ttcatggcag cgcaggagcg aagattcaat attgttgtca agcacaccga agatgagata
     1561 tcagccataa atatggcaat aggcgccgga tttacaggcg caagggcaat gtgcggcaca
     1621 tccggcggcg gattctgtct gatgtctgaa ggttatggtc ttgcaggcat gcttgaagtc
     1681 cctattgtaa tagtaatgtc tatgcgtccg ggtcccagca ctggcatgcc gacctggtcc
     1741 gaacagggcg acctgaaatt cgtgttgaat gcttcccagg gcgatttccc gcggcctgtg
     1801 cttgttcccg gagatatcga agaaagtttc tatttgaccg gagaagcatt caacattgca
     1861 gaaaaatatc agacccctgt tattgtcctg agtgacaaga accttgcaga aagccacaag
     1921 accacggaaa aatttgatga aaaaaagatt gccatagacc gcggaagctt cgcatcacca
     1981 gatgatctgg ctgcatcttc cgatacgact ttttttaaga gatataaata tacggaaaac
     2041 ggcatttctc caagaactat tccggggatg aaaaaaggaa tatatacagg tgcaagcgac
     2101 gaacatgatg aagaggggaa catcagggaa gacgctgata tcaggattaa aatgatgcaa
     2161 aaaagagggc ggaaaatgat ggcactttca aaagaattga agcagcctga attggcaggg
     2221 ccaaatggag ctgatatcac gataattgca gcaggttcca caaaaggccc gatcaaggaa
     2281 gctatgatat tgcttgaaaa ggagggtttc aaagtaaatt atctccagat agtatattta
     2341 aatccatttc cctctgaaaa ggtggcaaaa gtaattgaat ccgcaaaaaa gacggtggtc
     2401 gtggaaaaca atttttcggg acagcttgct gatattatta aagagaaaac aggcagggat
     2461 gtaaataaga gggtattgaa atatgacggg cgccccttct atccggaaga aatagttact
     2521 gcaataaagg aggtggctca tggttaatat gactgtcctg aatactattg ataagcccaa
     2581 ctggtgtccg gggtgcggcg atttcgggat cctggtagcg ctcaaagggg cagtagttga
     2641 acttaatatc gagcaggaaa atatcgtgtt ggtttcaggg atcggctgtt cgggaaaatt
     2701 gcctcactgg atacggacat atggcctcca tggcattcat ggaagggcat tgcctgttgc
     2761 cagtgctgtg aaactcgcga ataatgaatt gacagttttc gcagtgggcg gcgatggcga
     2821 tggatacggt atcggtatgt gtcatttcat tcatgcgatg cgaaggaaca tcgatatgac
     2881 atatatcgtg cacaataaca tgatctacgg gcttacaaca gggcagactt ctccaacaag
     2941 cgaaaaggga atgaggacaa aatccactcc atccggtgtg atcgaagtgc ccgtaaatcc
     3001 gatagctctt gcaatatcaa caggtgcgac gttcgttgcg cgaagttttg cgggtgatgt
     3061 aaagcatctg cagaaaacga tagtggatgc tgtcaagcac aggggtttct cccttgttga
     3121 cgttttccag caatgcgtga gttttaacca tgtgaatact tatgactggt ataagcaaag
     3181 ggtgtatgac ctatcaactg ccgggcatga tccggcggat aagttgaagg ctttcacgaa
     3241 gtcgcaggaa tggggcgatc atatacctgt aggggtattt tataaagtgc tacagccaac
     3301 ttatgaggat gaactgccgc agataaagga taagccgctt gtgaagcagc ccatagataa
     3361 tatcgatatt ggagggctta tggatgattt tgtgtgatta tctcacaagc ggcgcgccaa
     3421 atgtaagatc ttcaagttcg atcacttttt tccacaactc tttgcactca caatcaagat
     3481 tcttgaaaac cgaagtgtcc tgcgttaccg agaaaattcg gatcgcttct gccacgtcct
     3541 tatcgcattt tccacaatta tgggggccgc gcattgaacc ggcgcctaca gggtctgaca
     3601 ttatgaccgt tccaggggac aattctttgc ccgccttaag tgcttcaacc gcgctccata
     3661 gccacggcgg ccggtaatcg ccgcgctcga acatttcatc cacaagtgtt cctttctgga
     3721 cattgcacag gttcatggat attgtccggg caaaaggtgc ggcatcattt attgaattta
     3781 tcatatcgtt catggcgatg ccctctgaaa gaaacggcgg cttctgcata agataggctt
     3841 ttaccgttac atcattgttc tttgcgttct ggcttgcgag agtaaaatca gaaaaagaaa
     3901 atcctttatt gatgcaatac tttcgtatca ggtcattact tgtttcaaga ccaattgcta
     3961 cttcaaattt cttcccaaaa gctgcccttg cat
//
LOCUS       DATGBN010000175         6204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_401809, whole genome shotgun sequence.
ACCESSION   DATGBN010000175 DATGBN010000000
VERSION     DATGBN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401809"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /gene="gatE"
                     /locus_tag="VKL21_04145"
     CDS             <1..1075
                     /gene="gatE"
                     /locus_tag="VKL21_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HMB44994.1"
                     /translation="ERKAAVSDKIIDVTSVFANTTSKVIQKALKGGVVYAVNLTGFSG
                     LVGKEIQPGRRLGTELSDRARKSGVGGIFHTDELPNYGITKEEVEALRKAVSAREMDC
                     VVMVADSKERSQGAMEAVMIRAKEALEFIPEETRRALPDGNSAYMRPLPGAARMYPET
                     DVTPVVITEERIKGIKLPELIGERKARYIKQFSLNEDLANQMARSVNSIIFDRIMQQV
                     PEANPNSVVRVLETILYELEKEGVEVNKITDDHLMQLFRLQSEGKIPNEAISNILKTI
                     SLKPDLYVYESAESLGIGGVKEGELETAVDKLIEEKMDFVKEKGLNAVGPLMGLIMKD
                     FRGKVSGGEVNRLLKKKISKKFS"
     gene            complement(1092..1367)
                     /locus_tag="VKL21_04150"
     CDS             complement(1092..1367)
                     /locus_tag="VKL21_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB44995.1"
                     /translation="MSEDTEYLRRIYPENFPSATLIGWIIAVLVFSLIFLIPYFYARF
                     VEPVLVSFICDRMIPFLMVRLHLQKPIGFINHILNIRIFKPRNGEHA"
     gene            complement(1544..1984)
                     /locus_tag="VKL21_04155"
     CDS             complement(1544..1984)
                     /locus_tag="VKL21_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HMB44996.1"
                     /translation="MADFRVTVSDSKSKLAYQVAVTGPAANKLIGKNIGDVVSGDIAG
                     MAGYTLKLTGGTDKDGFAMRGDLPGSARRKILVAGGVGYIPKAEGVKRRKSMRGHEIS
                     SDISQINTVIVEYGEKPITEIFVKKEGEEGKKEERKTERPKSRR"
     gene            2246..3760
                     /locus_tag="VKL21_04160"
     CDS             2246..3760
                     /locus_tag="VKL21_04160"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB44997.1"
                     /translation="MSLIIIIVSVTSLGYLIDTIPYTHAMFGSLDILIELFFVFASLS
                     IFTMTWFAYSQNKNDHTLFIGATFLIIGVLNLFHVLSYPFMPDFITPNSIAKATIFRD
                     VTQVITAPLFLISAYLFKDTLRLLNRYILVICAVLLSILPFTATHYLGFVLNEYPKMY
                     YSSGEPSTVRISLIVLSILITLYASYLYAKRLKVNEEQEIIYLIYGFNIIVISDLLIN
                     VFDFSGILLKGAGFYFVYLALHQSYIELPYKKLIIAEESLKVAKEEADHSNQVKSEFL
                     SNMSHELRTPLNSIIGFSDLLKQKMMGELNGKQEHYIDNIIKSSKHLLDLINDILDLS
                     KIEAGKIEMTFEKVSLTALMNDTLELIRATATKHNVVIKKEFDPEIGFIEADKQKFKQ
                     ILFNLLSNAVKFSKTNGGTITITTKKNGEMVEFSVSDTGIGIKKEDLAKLFNKFQQID
                     SGISGKYGGTGLGLAISKQLVELQGGRIWVQSKYGEGTTFIFQLPLTTKKKVGN"
     gene            3762..4226
                     /locus_tag="VKL21_04165"
     CDS             3762..4226
                     /locus_tag="VKL21_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB44998.1"
                     /translation="MADMIDDKNSGEKTSESMIKPKILVVDDEPMNVEIFEGYLSKDY
                     DVVKAYNGDEALLKVGTTNPDLILLDVMMPGKNGFEVCKILKENPKTMAIPIIIVTAL
                     KEKEDRIKAIEAGADDFLSKPIDIIEVTARVKSLLRIKQYYDALMGDCHFLL"
     gene            4248..5240
                     /locus_tag="VKL21_04170"
     CDS             4248..5240
                     /locus_tag="VKL21_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMB44999.1"
                     /translation="MEKNLIRKFSIYSILIIAILGIVLNLVISSRIENMVIERSEITK
                     LQMTIAIIIFLGLFTLYFSLIWIVKDASETIMEQNLALIKSHEELKEMDKMKNEFINS
                     MSHELRTPLNAVIGFSDLMKQKNAGELNERQEHYIDNILKSGKHLLTIVNDILDMIVI
                     EAGKTELVLSTFSVPFAIDENIECVTSNTEKNNVIINKEIDPQLESIEADEQKFSQIL
                     VNILDNALKFSKKDGGVVNIIARKTGDNAQFSISDNGIGIKEENFKKLFMVFQQLDTG
                     ISRKYGGTGLGLSISKKLVELHGGKIWVESKYGEGTTFTFELPLAQKKEVKPND"
     gene            5233..5598
                     /locus_tag="VKL21_04175"
     CDS             5233..5598
                     /locus_tag="VKL21_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45000.1"
                     /translation="MTKVLVVEDNPLNMELVVEILNSLGFEVFGAINGEDAIKEVEKE
                     HYDLILMDIELPVMDGISALRIIKSKPEYHNVHTVALTALAMKGDKEKFLAAGFEDYI
                     SKPIDVPEFMKKMLIFKKS"
     gene            5638..>6204
                     /locus_tag="VKL21_04180"
     CDS             5638..>6204
                     /locus_tag="VKL21_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45001.1"
                     /translation="MENQLSRAKVLVVDDEPLNIELISGYLEKEFEIIPAYSGKEALE
                     KVRLANPDIVLLDIMMPEVKGFEVCEKIRTQDLTRFTPIVMITALTEIEDKIKAIESG
                     ADDFLTKPINRIELVTRIKSLLKTKYYHDQLVKSMEQIEVQNEFKTIMTDILPLIFQS
                     IPPGKMTEVIGQMSKRVEDVIWAKYIKEI"
BASE COUNT         2127 a         1074 c         1246 g         1757 t
ORIGIN      
        1 tgaacgcaaa gctgcagttt ctgataagat cattgacgtc acatccgtgt tcgcaaacac
       61 tacatcaaaa gtaattcaaa aagcccttaa aggcggcgta gtatatgccg tgaatctcac
      121 gggattttcc ggtttagtag gaaaagaaat acagccggga aggcgcctgg gcacggagtt
      181 atcagaccgc gccaggaaat ccggggtagg ggggatattc cataccgatg aattgccaaa
      241 ttacggcatt acaaaagagg aagtagaagc gctcaggaaa gctgtaagcg cacgggaaat
      301 ggattgtgtg gtcatggtcg ctgattcaaa agagagatca cagggggcaa tggaagctgt
      361 catgatccgc gcaaaagaag cgctggaatt catccctgaa gaaacaagac gcgcgctgcc
      421 tgacgggaat tcagcgtata tgagaccgct gcccggagct gcgcgcatgt atcctgagac
      481 cgatgtcacg ccggttgtga tcacagagga gagaatcaag ggaataaagc ttcctgaatt
      541 gatcggcgag cgaaaagcaa gatacataaa acaattcagc cttaatgaag atcttgcgaa
      601 ccagatggct cgctcggtaa actccattat ttttgacagg ataatgcagc aggtccctga
      661 agcaaatcca aattcggtcg tgcgtgtgct tgaaactatc ctgtatgaac ttgaaaaaga
      721 aggcgttgag gtcaataaaa ttaccgatga tcacttgatg cagcttttca ggctccagtc
      781 ggaaggaaag atccccaacg aagcaataag caatatcctg aagacaatat ccttaaagcc
      841 cgacctgtat gtgtatgaat cggcagaatc tcttggcata ggcggggtga aagaagggga
      901 actggagact gctgtcgata agctcattga ggaaaagatg gattttgtta aagaaaaggg
      961 cttgaatgcc gtagggccgt tgatgggctt gatcatgaag gatttcaggg gcaaagtaag
     1021 cgggggagag gtcaacaggc tgttgaagaa aaagatatca aagaaatttt cctgatctgc
     1081 aaatattatt ttcaggcatg ttctccattt ctgggtttga atatccggat attgagaata
     1141 tgattaataa aaccgatcgg tttttgaaga tgcaacctga ccatgagaaa aggaatcatc
     1201 ctgtcacaaa taaaagaaac cagtactggt tcaacaaacc ttgcataaaa atacgggatc
     1261 agaaaaataa gactgaaaac caaaacggct atgatccaac ctattaacgt agcagaggga
     1321 aaattttcag gataaattct tctcaggtat tctgtatctt cactcattaa tgtaatatat
     1381 gtttctatta taaatagtct ttgcgctcat atatatacta ttttcgcagg ataggctgag
     1441 aaggggggtt ataactcttt aaaaagatgt agaggaagat gagggggaaa aaagaaaatc
     1501 aggatagcgt ttgaaaaaat ttccagaacg ctattattta agattatctt ctggattttg
     1561 gtctttcagt cttacgttct tccttcttac cttcctcgcc ttctttctta acgaagattt
     1621 cggtaatagg tttctcgcca tattccacaa taacagtatt gatctgggag atatctgatg
     1681 atatctcatg tcctctcatt gattttcgtc tttttacacc ttctgccttt ggaatataac
     1741 caacgccgcc tgcaactaat atctttcgtc ttgccgatcc tggtaaatcc cctctcatcg
     1801 cgaagccgtc cttatcggtt ccgcctgtca gcttgagggt gtatcctgcc atcccggcaa
     1861 tatcgccact cacaacatcc ccgatatttt ttcctatcaa tttattagct gccgggccgg
     1921 ttacagctac ctggtatgcc aatttactct tactgtccga aacagttact ctgaaatctg
     1981 ccattcttta tattctcctt attaataata ataatctatc ccttaaatag gaatatatca
     2041 attcttgact ttacttacag ttaatatact taaacatatt gcatgttgca tgtgtgacac
     2101 cttcctcaca actgtactta agacattgtg gattcaaaat atcatttgaa atatcaagat
     2161 tattagaata gctaaaatat attgtaatta cgattcgata acaacaaatc aggaattaaa
     2221 aatgagagga actctgaaat atgtcttgag tttaataata ataattgtat cggttacttc
     2281 cctaggatat cttatcgata caattcccta cactcatgcc atgtttggtt ctcttgatat
     2341 cttaatagaa cttttttttg ttttcgcttc cctctcaatt tttacaatga cctggttcgc
     2401 atacagccag aataagaatg accataccct ttttatagga gccacttttt taataatagg
     2461 tgttttaaat ctatttcatg tactttctta tcctttcatg cctgatttta taactcccaa
     2521 ctccattgct aaagcaacaa ttttcaggga tgtgacccag gtaattaccg cacccttatt
     2581 tctaattagc gcatacctgt tcaaggatac tctcaggttg ctaaacaggt acattcttgt
     2641 aatttgcgca gttttacttt ctatattacc gtttaccgca acacattatc tcggattcgt
     2701 gttgaatgaa tatccaaaaa tgtattattc aagcggtgaa ccctctacag taaggatatc
     2761 gttgatcgtg ttaagcatat tgatcacttt atatgcaagt tacctgtatg caaaaagact
     2821 aaaggtaaat gaagaacaag aaatcatata tctcatatac ggattcaata tcatagtaat
     2881 tagcgacctt ttaataaatg tattcgactt ctcaggaatt ttactaaaag gcgctggttt
     2941 ctattttgtg tatcttgccc ttcatcaatc atatattgaa ctaccatata agaaattaat
     3001 aattgctgaa gagtcactta aagttgcaaa agaagaagct gatcattcaa accaggtcaa
     3061 atccgaattc ctgtcaaata tgagccatga acttcgaaca cctcttaatt ctatcatagg
     3121 cttttctgac ctgctaaagc aaaaaatgat gggagaatta aatgggaaac aagaacatta
     3181 tattgataat attatcaaaa gcagtaaaca tcttctcgac ctgataaatg atattcttga
     3241 cttaagcaag atagaagctg gaaaaatcga aatgaccttt gaaaaggtgt ctttaacagc
     3301 attaatgaac gataccctgg aactcataag agctaccgca acaaaacaca atgtagttat
     3361 taaaaaagaa tttgatcctg aaattgggtt catagaagcg gataaacaga aatttaaaca
     3421 gatacttttt aatcttctga gtaacgctgt caaattcagc aagacaaatg gaggaactat
     3481 aacaataact accaagaaaa atggtgaaat ggtagaattc tctgtatccg acacaggtat
     3541 tggcattaag aaagaagatc tggcaaaact tttcaataag ttccagcaga tcgattcggg
     3601 catttccgga aaatatggtg gcacaggtct tggccttgct atttcaaaac aattggtgga
     3661 gcttcaggga ggcaggatat gggtacagag caaatacggt gaaggaacca catttatctt
     3721 tcagttgcca ctaacaacga aaaagaaggt tgggaattaa tatggcggat atgattgatg
     3781 ataaaaatag cggtgagaaa acaagtgaaa gtatgattaa accaaaaata cttgttgtcg
     3841 atgatgaacc catgaatgtt gagatttttg aaggatattt atcaaaggat tacgatgttg
     3901 ttaaagcgta taatggagat gaagctctcc ttaaagtagg aacaacaaat cccgacctta
     3961 ttcttcttga tgtaatgatg ccaggaaaaa acgggtttga ggtatgcaaa atacttaaag
     4021 aaaatccaaa aacaatggca attccgatca ttatcgtgac cgcgctgaaa gaaaaagagg
     4081 ataggataaa ggccatagaa gcgggagctg atgatttcct gagtaaacct attgatatta
     4141 tagaagtgac cgcaagagtg aaatcactat taaggatcaa gcaatattat gacgctttaa
     4201 tgggagattg ccatttctta ctttaaaaca catagaacta tataattatg gaaaaaaatt
     4261 taatcagaaa attttcaatt tatagcatat taataattgc aattcttgga atagtgttga
     4321 acctggtgat atcctccagg atcgaaaaca tggtcattga aaggtcagaa atcaccaaat
     4381 tacagatgac aatagcgatt attatttttc tgggtttatt cacattatat ttttcattaa
     4441 tatggatagt aaaagatgct tctgaaacaa taatggaaca gaatttagcc ctcattaagt
     4501 cacatgaaga acttaaagaa atggataaga tgaaaaacga atttataaat tccatgagcc
     4561 atgaacttcg aactccattg aacgcagtca tcggattttc ggatctcatg aaacagaaga
     4621 atgcagggga acttaatgaa aggcaggagc attatattga taatattttg aaaagcggga
     4681 agcatctgct taccattgta aatgacattc tggatatgat cgtaatagaa gctggaaaaa
     4741 cagaattggt attaagtact ttctccgtgc cttttgctat agatgaaaat attgaatgtg
     4801 tgacatccaa cacagaaaaa aataacgtaa ttatcaataa agaaatagat cctcaattag
     4861 aatctattga ggcagacgag cagaaattct cccagatatt agttaatatt cttgataatg
     4921 cattaaaatt cagtaaaaag gatggaggag ttgtaaatat aattgcaaga aaaacaggag
     4981 ataatgcgca attttccata tcagacaacg gaatagggat caaggaagaa aactttaaga
     5041 aactattcat ggtattccag cagcttgata cgggtatttc caggaaatac gggggaacgg
     5101 gacttggact ttcaatttca aagaaactgg ttgaattaca cgggggcaaa atatgggtgg
     5161 aaagtaaata cggtgaagga actacattca catttgaatt gcctctggcc caaaagaagg
     5221 aggttaaacc aaatgactaa agttctcgtg gttgaagata atcccctcaa tatggaactt
     5281 gttgttgaaa ttctcaattc tttaggattt gaagtatttg gggcaattaa tggggaggat
     5341 gcgatcaaag aggtcgaaaa agaacattat gatttgatcc tgatggatat agaactaccg
     5401 gtgatggatg ggatatctgc tttaagaatt atcaagagca agccggaata ccataatgtg
     5461 cacactgtgg ctttgactgc tttagctatg aaaggggaca aagaaaaatt tttggctgcg
     5521 ggttttgagg attatatttc aaaaccaatt gatgtacccg aattcatgaa aaaaatgcta
     5581 atatttaaga aatcctgata tgaaacaaaa taccattctt ttttggggtt gcatttaatg
     5641 gaaaatcaat tatccagggc aaaagtcctt gtggttgatg atgaaccttt aaatatagag
     5701 ctcatttcag gatatctcga aaaggaattt gaaataatac cggcatacag tggaaaagaa
     5761 gcccttgaaa aagttcgcct ggctaatcct gatatcgttc tactggatat tatgatgcca
     5821 gaagtgaaag gatttgaagt atgtgaaaaa ataaggaccc aggatttaac acgattcacc
     5881 cccatagtta tgatcacggc attgactgaa atagaagata agatcaaggc catagaatcc
     5941 ggggcggatg atttcctcac taaacccatc aacaggatag aacttgtcac aaggataaaa
     6001 tcacttctga aaacaaaata ttatcatgat caactagtaa aaagcatgga acaaattgag
     6061 gttcaaaatg aattcaaaac tattatgacg gatatacttc ctcttatctt ccagagcatt
     6121 ccccctggaa aaatgacgga agtaataggc cagatgagta aacgggtaga agatgttatc
     6181 tgggctaaat atattaaaga gatt
//
LOCUS       DATGBN010000176         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_324231, whole genome shotgun sequence.
ACCESSION   DATGBN010000176 DATGBN010000000
VERSION     DATGBN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324231"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VKL21_04185"
     CDS             complement(<1..1080)
                     /locus_tag="VKL21_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HMB45002.1"
                     /translation="MMSANRKLLFVLATVIFALGIIASAGTISDAAKESGDVAKSKII
                     EEGIQEGSETEVLAYLYDKHINGGYTASGTGMRNRGWGTILIKDIPKGSTVDRAYLYW
                     SVIGPAVPGRIPRSYAQGKINGVSINGTYLGFAPSLCWGKGQIYGYRANVTSLVTANN
                     QYHLQDFASGYKWGDIEPSGKNYPLMEGASLVVVYTNAKYPLTTIQIKDGIRNVVNNS
                     TVFSGFPGYNLSLAKTTYIVSGGQNGTESVKFNGIEMPSYFLDGKDRQNGMNFKSGNL
                     QDTNTSYVKVTPRATMAKATIVKGRDCLAWVAQVFSISDGNLDTDGDALKDAWEVYGY
                     DYDRDGITDVNLPASGADPFHKDIFV"
     gene            complement(1241..2620)
                     /locus_tag="VKL21_04190"
     CDS             complement(1241..2620)
                     /locus_tag="VKL21_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMB45003.1"
                     /translation="MNEDINKKIVLLITTLSSFLAPFMGSSVFIGLPSIGKEFAMDAV
                     TLSWISTSYLLAAAMFLVPFGRIADIYGRKKIFKYGIIIDIIASLLLAFSFNETEFII
                     LRFLQGIGAAMIFGTGIAILTSVYPPGERGKALGITVAGVYCGLSLGPVIGGFLVQHF
                     GWRSIFLSYLPLELIIIVLTLWKVKGEWADARGEKLDITGSAVYCLSLVAMMYGFSVL
                     PDIFGYGLILLGIIGIAIFISWELKVKAPVLDMNLFRGNTVFTFSVLAALINYSATFA
                     VSFFLSLYLQYIKGITPQDAGLILVAQPIVMVVFSPLAGRLSDRIEPGIIASVGMAIT
                     AVGLFLFTFLDEKTTLVFIIFGLILVGFGFALFSSPNTNAVMSSVEKRFYGIASATLG
                     TMRLVGQMLNMGIAMIIFAVFIGRVEITPEYYPPFLKSVNLAFMIFGVLCFAGIFASL
                     ARGKLIRNN"
     gene            complement(2629..>4006)
                     /locus_tag="VKL21_04195"
     CDS             complement(2629..>4006)
                     /locus_tag="VKL21_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMB45004.1"
                     /translation="VGILNASTLIIALPTMMVKLNTDLFGIMWVLISYTLLLTILAPA
                     WGRLADIYGRKKLYVYGLAVFTVGSLLCGLAANINQLIAFRVLQAVGGSMLVANGTII
                     VTDAFKRNELGKAMGILSMVMAAAFVVGPILGGFLTLIDWRLNFFFNVPIGIVVTIWA
                     HLKLKEVAVLPKGETFDLKGMVLFTIAFLGFMIYLTAGFIVGLTSLPMLLSLVIAIVS
                     FTAFLRVERRTAYPLMDLNLFKIKIFSYGQLSNLLNSIARGAVMILLILFFQGPRGFD
                     PLWASILITPLAIGLAITGPIGGVLSDKYGSRIISTVGLVISLVGLVGLTTIHYNTPY
                     WIIAVWMFINSFGSGLFQPPNTSAIMSSVSPQRRGVASSMRAFFNSAGMVLSMGIAFP
                     LIMGTISIDEMMNMFVVGGANMPVVVQEAFTSGITGAFILSAIITVPAIIASALRGKE
                     NNSDNSIE"
BASE COUNT         1193 a          967 c          770 g         1076 t
ORIGIN      
        1 tacgaatata tctttgtgga atggatcagc tccggaagca ggtaaattaa catccgttat
       61 tccatcgcgg tcgtagtcat atccatagac ctcccaggca tccttcaatg catcgccgtc
      121 cgtatccaga tttccatctg atattgaaaa tacctgagct acccatgcaa ggcaatctct
      181 tccctttaca atagtagctt tggccatggt tgcacgagga gttaccttta cataagacgt
      241 atttgtatcc tgcaaatttc cagatttaaa gttcatccca ttctgcctat cctttccatc
      301 caggaaatat gaaggcatct cgatcccatt gaatttcact gactctgtac cattttgacc
      361 gcccgaaaca atataggtgg tctttgccaa tgagagatta tatcccggaa atcctgaaaa
      421 aaccgtacta ttatttacta cattccgaat tccgtctttt atctgaatag ttgtcagggg
      481 gtattttgca ttcgtatata ccacaacaag ggatgcccct tccattaagg gataattttt
      541 tcccgatggt tctatatctc cccacttata acctgaagca aaatcctgga ggtgatattg
      601 attgttcgca gtaactaaac ttgttacatt tgccctgtat ccatatattt gcccctttcc
      661 ccagcataaa gagggggcaa aacccaggta agtgccatta attgaaacgc catttatctt
      721 tccctgcgca taagaccgtg gaattcttcc cggtactgcc ggcccgatta cagaccaata
      781 tagatatgcc ctatcaacag tagagccttt tggaatatct ttaataagaa ttgttcccca
      841 acccctgttt ctcatacctg tcccacttgc agtatagccg ccattgatat gcttatcata
      901 caaatatgcg agtacttctg tttcagatcc ctcctgtatt ccttcttcaa taattttgct
      961 tttagcgaca tctccgcttt ctttggcagc gtccgaaatt gttcctgcac ttgcaatgat
     1021 acccaaagca aaaattacag tagctagaac gaataataat ttcctattag cactcatcat
     1081 tttctcctga tttcacactt tctctcaatt tttctatctc aatcccacaa ttgagacagg
     1141 gatgaagtca aaaataagag ttcagccacc atatactttg tggttaatga actcaatatt
     1201 tatagttggc accacaacct atatttaatt tgatattttt ctaattattt cttattaact
     1261 tacctcttgc aagtgatgcg aatatacctg caaaacataa aacaccaaaa atcatgaagg
     1321 ctaggttcac gcttttcaag aaaggcggat aatactcagg cgtaatttcc actctcccta
     1381 tgaatactgc gaatatgatc atagcaatgc ccatgttaag catctgtcct accaggcgca
     1441 tcgttccgag tgtggctgaa gctatgccgt agaatctctt ttcaactgaa ctcatgaccg
     1501 cattcgtatt gggagatgag aaaagcgcaa agccaaaacc gacaaggatc aaaccgaata
     1561 tgatgaaaac caaagtggtt ttttcatcca gaaaagtgaa aaggaaaagc cccaccgcag
     1621 taatagccat tcccactgaa gcaattattc ccggttcgat cctgtctgaa agcctccctg
     1681 caaggggtga aaaaacaacc atcacaattg gctgggctac aaggatcaat cccgcatcct
     1741 gcggagtgat acctttgata tactgcaggt aaaggcttaa aaagaatgat accgcaaatg
     1801 ttgcgctgta gtttataaga gccgccagga cagagaacgt gaagaccgta ttgcctctaa
     1861 aaaggttcat atcaagaaca ggagctttta ccttcagctc ccaactgata aatatcgcaa
     1921 ttcctatgat gcccagcagg atcagaccat acccgaatat atccggcagc accgaaaaac
     1981 catacatcat cgccacaagc gagaggcagt aaactgcgga accagttata tctaacttct
     2041 ctcccctggc atcagcccac tcacctttca ctttccagag agttaaaacg attattatca
     2101 gttcaagggg aaggtacgaa agaaaaatac ttctccaacc gaaatgctgt acaaggaacc
     2161 cgcctatcac gggtccgaga gaaagcccgc aataaacccc tgcaactgtt attcccaaag
     2221 cttttcctct ttcccctgga ggatatactg aggtcaggat agctattccc gtgccgaata
     2281 tcatcgctgc gccgataccc tgcaaaaacc gaagtataat aaactcagtt tcgttgaatg
     2341 aaaaagctag aaggagagag gctattatat caatgattat tccgtatttg aaaatcttct
     2401 tcctgccgta tatgtccgcg attctcccga acgggacgag gaacatggca gcggccaaaa
     2461 gatatgatgt agaaatccag cttaaagtga cagcatccat tgcaaattct ttgccgatgg
     2521 aaggaagacc gatgaacact gaggagccca taaagggcgc aagaaaagaa ctcaatgttg
     2581 tgatcaacaa cacaattttt ttgtttatat cctcgttcat atatcctctt attctattga
     2641 attatcacta ttattctctt tcccccttaa tgcagatgca ataattgcag gcacggtgat
     2701 tatcgcagac agaataaatg ccccggttat accgctggta aaggcttcct gcacaaccac
     2761 aggcatgttc gccccaccta ccacaaacat gttcatcatc tcatcaatag agatcgttcc
     2821 catgatcaat gggaaagcta ttcccatgct caggaccatg ccggcgctgt taaaaaatgc
     2881 cctcatggat gaggcgaccc cgcgccgctg cggggatact gatgacatga tcgcgctagt
     2941 gttcggtggc tggaacaaac ccgaaccaaa gctgtttatg aacatccata ctgcaattat
     3001 ccagtaaggc gtattgtaat gaattgttgt aagacccaca agaccgacga gggaaattac
     3061 caatcccacc gtgctgatga tccttgatcc atatttatct gaaagcacac ctcctatcgg
     3121 acctgttatt gcaagaccga tagcaagggg agttatgaga atacttgccc aaagcgggtc
     3181 aaagcctctt ggtccctgaa agaaaagaat aaggaggatc atcactgctc ccctggctat
     3241 ggaattcagg aggttactta gctgtccgta tgaaaaaatt tttattttga aaaggtttaa
     3301 gtccatcaac ggataggcgg ttcttctttc aactcgcagg aaggcggtaa aactcacgat
     3361 cgcgataaca agcgagagaa gcatcgggag tgatgtcagt ccgacgatga atcccgctgt
     3421 gaggtatatc atgaatccga gaaaagcgat agtaaataag accattcctt taaggtcgaa
     3481 tgtttcacct tttggcagca ctgcaacctc ttttagtttg agatgcgccc atattgtgac
     3541 aactatgcct atcgggacat taaaaaagaa gttcagccgc cagtctatca atgttagaaa
     3601 acctcccagt atcggaccca cgacaaaggc tgctgccatt accatgctaa gaattcccat
     3661 tgcttttcca agttcattcc gtttgaaggc atcagttact ataattgtgc cattggccac
     3721 gagcatcgac cctcctacag cctgcaatac ccgaaaagcg ataagctgat ttatgttagc
     3781 tgcaagaccg caaagcaatg aaccgaccgt gaacacagcc agcccataaa cgtataattt
     3841 ctttcgcccg tatatatccg caagccttcc ccatgccggt gcgagtattg tgaggagaag
     3901 agtataactt ataagtaccc acattatccc gaaaagatct gtgttcagtt taaccatcat
     3961 tgtgggaaga gcgattatca gtgtgctggc attgagtatc ccgaca
//
LOCUS       DATGBN010000177         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_285207, whole genome shotgun sequence.
ACCESSION   DATGBN010000177 DATGBN010000000
VERSION     DATGBN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285207"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VKL21_04200"
     CDS             <1..269
                     /locus_tag="VKL21_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HMB45005.1"
                     /translation="ARTMNIRIKPRLYTLSTCVHCSALKRFLVENGIEYDYVDVDKLH
                     GKEKQDILSEMMKNTGDMRFPTIVIGKKIIVGFYEDKVREALGL"
     gene            266..763
                     /locus_tag="VKL21_04205"
     CDS             266..763
                     /locus_tag="VKL21_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HMB45006.1"
                     /translation="MRTIEDMFDEMKKIAQEEGYRFNPNKVELQEILQGMWDNEHRYG
                     YPSCPCRLASGELADDMDMICPCNYRDADIAEYGSCLCTLYVNDEWISGRKPHGVVPE
                     RRPQEYYIKGYPGIKEQKGAGGGEMVEVYRCQVCGYLCAREEAPDLCPICRAKKERFE
                     KFKIR"
     gene            892..1458
                     /locus_tag="VKL21_04210"
     CDS             892..1458
                     /locus_tag="VKL21_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMB45007.1"
                     /translation="MVTSIMTIFYFLLLLVIIIAIYSFSIYNRLKSLRNAADATLSQA
                     RVAMKKRIDMIEQLVEAVKSYAKFEHDTFEKITSMRVNVGKAGAGELKKIDGESRGIL
                     GNIIAVAENYPALKTSDTVTSLMNSIKDIEDEIARHRYTYNNIIQEYNTMLDTIPSRF
                     IGRSVGMSKKEYLDFFDEELKRPVVNWG"
     gene            1455..>1865
                     /locus_tag="VKL21_04215"
     CDS             1455..>1865
                     /locus_tag="VKL21_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45008.1"
                     /translation="MNENKEIVILLIVVALIGLSGLYLTGGISGTENACGLGGCGDVY
                     VDSYSANLYLNGTLEENFVYKIKEPFKYRMLYRNWKVPLSYGISNDPTLKTAPHVELV
                     SILPPPGSFPYIKNFQGITKIISDADSQYTNEISS"
BASE COUNT          606 a          304 c          467 g          488 t
ORIGIN      
        1 atgcacggac catgaatata agaatcaaac caaggctgta cacactgagc acatgcgttc
       61 attgcagcgc attgaaacgt ttcctggtag agaacgggat agaatatgac tatgttgatg
      121 tagataaact gcatggaaag gaaaaacagg atatactttc cgaaatgatg aaaaacacag
      181 gggatatgag attcccgacc atagtaatag gtaaaaagat cattgtgggt ttctatgaag
      241 ataaagtaag agaggcgctt ggcctatgag aactattgaa gatatgttcg atgagatgaa
      301 aaagatcgca caggaggagg gttacaggtt caatcccaat aaggttgaac tccaggagat
      361 actccagggc atgtgggaca acgagcatcg ctacggatat ccatcctgcc cctgccgtct
      421 tgcaagcggt gaacttgccg atgatatgga tatgatctgc ccctgcaatt acagggatgc
      481 agatatcgca gagtatggaa gctgcctgtg tacgctgtat gtgaatgatg aatggatatc
      541 aggaaggaag ccgcatggag ttgtgcctga gcggcggccc caggaatatt atattaaggg
      601 ttatccgggc atcaaagaac agaaaggcgc agggggtgga gaaatggtag aagtttacag
      661 gtgccaggtt tgcggttatc tatgcgcaag ggaagaggcg ccagatctat gtcctatttg
      721 cagggccaag aaggagcggt tcgagaagtt caagatacgt taaaggctat ggggtctgaa
      781 taaatggaac cgcagattga atactttgtc cattaaaatt aaatgaaaaa gaacggggtg
      841 gttttaaggt aaatagaaat aaatatatag aattaacctt aaatcaatga tatggtaact
      901 tcaataatga caatttttta ttttctgtta ttgctggtaa ttatcattgc catttattct
      961 ttttcgatat acaatcggct aaagagcctg aggaacgccg cggatgcaac gctatcccag
     1021 gcaagagttg cgatgaagaa gcgcatcgat atgatcgaac agcttgtgga ggctgttaaa
     1081 agctatgcga agttcgagca tgatacattc gagaaaataa cgagcatgcg ggtaaatgtg
     1141 ggaaaagctg gcgccgggga actgaaaaaa attgacggtg aaagccgcgg gatccttgga
     1201 aatattatcg ctgtggctga aaattatcct gcattaaaga cctctgacac tgtaacttcg
     1261 ctcatgaact ctattaaaga tatagaagat gagatcgcaa ggcacaggta cacatataat
     1321 aatataatcc aggaatataa tactatgctc gatactattc cttcaagatt cataggacgt
     1381 tcggtgggaa tgagcaagaa ggaatatctg gatttctttg atgaggaatt gaaaaggcca
     1441 gtggtgaact ggggttgaat gaaaataagg aaatcgtaat tttattgatt gtcgtagcat
     1501 tgattggttt gtcggggctt tatttgactg gaggcatatc gggcactgag aatgcatgcg
     1561 gtttgggagg atgtggggat gtttacgttg atagctacag tgcgaatcta tatttgaatg
     1621 gaacacttga ggaaaatttt gtttataaaa ttaaagagcc atttaagtac aggatgcttt
     1681 atagaaactg gaaagtaccg ctttcgtatg gtatatcaaa tgacccgaca ttaaaaaccg
     1741 ctcctcatgt cgaacttgtc agtatcttgc cgccgcctgg atcatttcct tatataaaaa
     1801 attttcaagg aataactaaa ataatatctg atgcagattc ccaatataca aatgaaatat
     1861 catct
//
LOCUS       DATGBN010000178         6944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_324556, whole genome shotgun sequence.
ACCESSION   DATGBN010000178 DATGBN010000000
VERSION     DATGBN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324556"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..970
                     /locus_tag="VKL21_04220"
     CDS             80..970
                     /locus_tag="VKL21_04220"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HMB45009.1"
                     /translation="MFSLTLTGLKLKNPLMLAAGIMGTTGGSLKRIADNGAGAVVTKS
                     LGIEPAGGHTNPTMVEVDCGYLNAMGLPNPSYKNFQQEIDIARCDGAPVIASIFGGCA
                     LDFSEIAGKLDADAFELNVSCPHARGYGAQIGTDPALVEDITKAVKAATDAPVWVKLT
                     PNVTDITSIGLAAQGGGADAVVAINTVRGMAIDINSGYPILGNRFGGLSGRAIKPAAI
                     KCVYDLYDALKIPVIGVGGISDWKDAIEFIMAGARAIEVGSAVGNNINVFKDISSGME
                     AFLMEKEWTLDEIYGMAHQK"
     gene            967..1710
                     /locus_tag="VKL21_04225"
     CDS             967..1710
                     /locus_tag="VKL21_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HMB45010.1"
                     /translation="MKPVNVVITKVVEETPTIRTFFFDTSFEPEPGQFVMVWIRGVDE
                     IPMALSCDNAITVQKVGQATSSLFELGEGDSIGIRGPFGNGFRIKGDNILLVAGGVGA
                     APLLPLAEKASSKGLKVATLLGAKTKEELIFRERFEAVGKVMVATDDGTAGKHGYVTQ
                     LIDGKNNYDQIYCCGPEIMMKKVLDILPEKSQFSLHRYIKCGLGICGACCVDGLRVCK
                     DGPVFTGEVLKKSEFGLYRRNECGEKVKV"
     gene            complement(1769..4471)
                     /locus_tag="VKL21_04230"
     CDS             complement(1769..4471)
                     /locus_tag="VKL21_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB45011.1"
                     /translation="MENTEKEEFPLILSPNLGCPKLISIKEVFSWDEKDINIKEFLIQ
                     KFGFDWIRTAKIEKFNNGMTIMVSGGKKFISMGLNNEKTTVNVKIDDGRITKFISRSE
                     NGKLNIYERVSINIILAGQYGELTSPTKEMFTDAFKLVSSYPGDFSMEIPLSIEEEIT
                     EITGWGQLFDFSHPEDTQMLINSEVHYNILGEGTKYFLIKAVIKTDEKEPEYETFLNT
                     GVEGKSIPTLYDLVFTDEPSHKKINYHAVQFIETSGKRLNFIHLTDLHVAQRNDDILG
                     EVLKNGDKGMRSRKQITERFINFNDNLRKFIYVANEMAQRGELDFIVITGDIVDFSGL
                     GWNECNNPAENNWKVFINLVTGQSTETSRRFRLQIDGNEETINPGLKVAIFTTTGNHD
                     WRLHPYGLLPLRLFSKTLQPGKNGEYGLTEEEAKKYNYKSFDSSEYPGDERKILADAI
                     MLQSLNRLNLKGMNWQDKGYILMANVLKYLSDNLKINSIATGLVGLISAYKFLDYKDI
                     FLKLSELLYNLTDIYAFFKIVLILLAALFIVGALSAFPFALNWIAQRMLKPLIDLMVD
                     NPIHAEARSLHYYFKHINPYFDYAFSFGNNHFVLMDTGADVAALRAENLREKVDSKYL
                     KKLTIQDNFIGSSPDSMAFDDRQSCYNWEQIIWLDKVLSAVSRKKSMAGKGRTFVCLH
                     APPVNYDTHYKSKLEELRESKLIEKNESSKTFIEAEKEPGMTPEMIMRIIDYIPQKLG
                     IKKKNDERTLNLTYGTVNHYLSQFFFMCMGYRENPHRNKNSRPLDQGQEKVDLILSGH
                     AHMDVEFRLGIDETDPDENKIRIYHDVYSDFEKFSDFDKNKPVIVQSAACGPPGENDK
                     KKDSNDEFDMNKLPHWRTVQVDEQNHITSFKQESLDEFLKNQKK"
     gene            4598..5047
                     /locus_tag="VKL21_04235"
     CDS             4598..5047
                     /locus_tag="VKL21_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMB45012.1"
                     /translation="MSKLAIIYGTGSHNTEKMAEAIEEGARAEGIETILKNVSCAHKS
                     DLIDADAIAIGSPNYRDLMMPSVQKFLNELADVSLSGKIGLAFGSFGWGLEAIEAIKE
                     QLISYGVEMIKDLCIKRMPGEEELEICRSVGSFLACKINDKEVKCAA"
     gene            5035..5190
                     /locus_tag="VKL21_04240"
     CDS             5035..5190
                     /locus_tag="VKL21_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45013.1"
                     /translation="MCSVGDSFGVELEGGMEPHLYVCNHCSKTFKGIGTNLKCPKCFS
                     KDVKCIE"
     gene            complement(5597..>6944)
                     /locus_tag="VKL21_04245"
     CDS             complement(5597..>6944)
                     /locus_tag="VKL21_04245"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HMB45014.1"
                     /translation="HLNLKLADEHLPYNKVIIVVHDPQGYVSQLFEHSLMDVKKEGDT
                     WIVRGASRKDSLFEIEMLLDPAISTEMDGFPNSMSGVKEKTIQANSKYSFFSQLSFAL
                     KVLVFLFPVMLAFVYYRFGREKSFIVPKFLSFVPRNRKPWLVNLVFRKDPFDYDENGF
                     YATLLDLHKRDIIKIETGGEKKLKIKMLGFKVVEDEGLRITLMKGHVAGEDEYEIKVL
                     KFLEEYSSDNVFESSKFEDEIEELRNSITGGSSSAQDELSDIRKSMNNVMRVPEKKAA
                     EEFVKSGKKYTWMIFGLFLFVLLVTSFLFSYGIYTSLVLLAQTIPPLFVSSSLFGRWK
                     ENYYKEKLEWDAFKTFLTDFASIKKYAPDDLKIWQEWLVYATTLGVGDKVVEAMKELN
                     IQIPEARSVTYMPIYFRGAYGMTSPPSPPSSGSGGFSGGDGFGGGGGFGGGGGGAR"
BASE COUNT         2014 a         1410 c         1418 g         2102 t
ORIGIN      
        1 ttgttgccga tttgaatcaa gggtgtcccg ttcacctcat ataaaaaaag ctaagtacgg
       61 tctgaacagt gttagcctga tgttctcact aaccttaaca ggcctgaaat tgaaaaatcc
      121 gctcatgctt gcagcgggga tcatgggaac gacaggcggc tcactcaaga gaattgcaga
      181 caatggggca ggcgcagtcg ttacaaaatc ccttgggatc gaacccgcag gcggacatac
      241 taatccgacc atggttgaag tggattgcgg ttatctgaat gctatgggtc ttcccaatcc
      301 ttcttataag aatttccagc aggaaattga tattgcaaga tgtgatggcg cgcctgtgat
      361 cgcaagtatt ttcgggggat gcgctcttga tttttcagaa attgcaggga aactggatgc
      421 tgatgcattt gagctgaacg taagctgtcc ccatgcccgc ggttatggtg cacagatcgg
      481 tacggacccg gctcttgtcg aagatattac aaaggcagta aaagccgcga ctgatgcgcc
      541 tgtatgggta aaactcacac ccaatgtcac tgatataaca agtataggat tggctgccca
      601 aggcggtggt gccgatgcgg ttgttgcgat aaataccgtg cgtgggatgg caatagatat
      661 caatagtggt tatcccatat tgggaaacag gttcggaggg ctatcaggcc gcgcaatcaa
      721 acctgctgcg atcaaatgcg tttatgatct ctatgatgcc cttaagatac ctgtgatagg
      781 agtgggcgga atatccgatt ggaaagatgc aattgagttt atcatggcag gagccagggc
      841 gatcgaagtg ggctcagccg tcgggaacaa catcaacgtt tttaaggata tttcctctgg
      901 tatggaagct tttcttatgg aaaaagaatg gacacttgat gagatatacg ggatggcaca
      961 ccaaaaatga aaccagtgaa tgtcgtaata acaaaggtag tggaagaaac gccaactatc
     1021 aggacttttt tctttgatac ttcttttgaa ccggagcctg gacagttcgt tatggtctgg
     1081 atacgcgggg tggatgaaat ccccatggct ctatcctgtg ataatgcgat aacggtccag
     1141 aaggtggggc aggcaacatc ttcattgttc gaacttggtg aaggtgactc tataggcatc
     1201 cggggacctt tcggaaacgg tttccggatc aagggtgata atattttgct ggtagcaggg
     1261 ggtgtgggag cggcccctct tttgcctctt gctgaaaagg caagttcgaa gggattaaag
     1321 gtcgcaacac ttcttggcgc gaagacaaaa gaagaactga tattcaggga acgctttgaa
     1381 gcggttggaa aggtgatggt ggctacagat gacggcacgg ctggaaaaca cgggtatgtt
     1441 acccaattaa tagacgggaa gaataactac gaccagatat actgctgtgg tccggaaatt
     1501 atgatgaaaa aagttcttga tatcctgcct gaaaaatccc aattcagcct tcaccgctat
     1561 atcaaatgcg gcctcgggat ttgcggggca tgctgtgtcg atggcctgcg cgtatgtaag
     1621 gacgggcctg tgttcacagg tgaggtttta aagaaaagcg agttcggact ttacaggaga
     1681 aacgaatgcg gggagaaagt gaaggtatga aaatccagtg attgaagatt tgaaattaaa
     1741 tgaacggaaa tatcgaaaaa tttgaaactt attttttttg atttttaaga aactcatcca
     1801 gactttcttg cttgaaactt gtgatatgat tttgttcatc gacctgaact gttctccaat
     1861 gaggaagctt attcatgtca aattcgtcat ttgagtcctt ctttttatca ttttcaccgg
     1921 gaggtccgca ggccgcgctc tgcacgataa caggcttatt tttatcaaaa tcagaaaatt
     1981 tttcaaaatc actgtaaaca tcatgataga tccttatttt attttcatca ggatctgttt
     2041 catcaatacc aagcctgaat tctacatcca tgtgagcgtg tcccgatagg atcaggtcga
     2101 ccttctcctg accctgatcc agaggacgcg aatttttgtt tctatgagga ttttcacgat
     2161 aacccatgca catgaagaag aattgactca ggtaatgatt tactgtgcca taggtgaggt
     2221 tgagggttct ctcatcattt tttttcttaa ttcctaattt ttgtgggata taatcaataa
     2281 tcctcattat catttcaggc gtcattccgg gttctttttc tgcttctata aatgttttgg
     2341 aactttcatt tttttcaatc aatttacttt cacgcagttc ctccagttta cttttgtaat
     2401 gtgtatcgta gttcacaggg ggcgcatgaa ggcagacgaa ggttctgcct ttcccagcca
     2461 tactcttttt gcggctgacc gcagaaagca ccttatccag ccatattatc tgttcccaat
     2521 tatagcacga ctgcctatca tcgaacgcca tggaatcagg agaggaacct atgaaattat
     2581 cctggatggt gagtttcttc aaatatttcg agtctacttt ttccctgaga ttttcagccc
     2641 gcagggctgc tacatcggcg ccggtatcca tcaaaacgaa atgattattt ccaaaactga
     2701 aagcatagtc aaaatagggg tttatatgct tgaaataata atgcagtgat cttgcttcag
     2761 catgaatggg attatccacc atcagatcga tcaatggttt caacatacgc tgagctatcc
     2821 aattcagtgc aaaagggaat gcagataatg ccccaacaat aaataaagcc gccaatagaa
     2881 tgagaacgat cttgaaaaat gcataaatat cagtcagatt atataataac tccgacaact
     2941 tcaaaaatat atctttataa tccaaaaatt tatatgcgct tatcaagcca acaagaccgg
     3001 ttgcaatgga atttatcttt aaattatcac tcaagtattt caatacattc gccatcaaaa
     3061 tatacccttt atcctgccaa ttcattcctt tcagattcaa tctatttaaa gattgcagca
     3121 ttattgcatc cgccagtatc ttacgttcat caccaggata ttcagaagaa tcaaaggact
     3181 tgtaattgta cttctttgct tcttcttcag tcagaccata ttctccattt tttcccggct
     3241 ggagagtttt agaaaatagt cgcaatggaa gcaatccata tgggtgcagg cgccaatcat
     3301 ggtttccagt agttgtaaat attgctactt ttaatcctgg attaatagtc tcttcattac
     3361 catcaatttg cagccgaaaa cgtcttgatg tctctgtaga ttgcccggtc actaaattta
     3421 tgaatacttt ccagttgttc tctgcagggt tattgcattc attccagccc aatcctgaaa
     3481 aatctacaat atctcctgta ataacgataa aatccagctc acctctttgt gccatttcat
     3541 ttgcgacata aatgaacttg cgaagattat cattgaaatt gataaatctc tctgtgatct
     3601 gttttctgct gcgcattcct ttgtcaccgt tttttaacac ctctcccaaa atatcatcgt
     3661 tcctttgtgc aacatgaagg tcggtcaaat ggatgaaatt taagcgcttt ccagaggtct
     3721 caataaattg aactgcatga tagtttattt tcttatgcga gggttcatcg gtaaaaacaa
     3781 gatcgtagag ggttggtata gattttcctt ccactcctgt attcagaaag gtttcatatt
     3841 ccggttcttt ttcatctgtt ttaattactg ctttgataag aaaatatttt gtcccttctc
     3901 ccaaaatatt atagtgaact tcactattta ttaacatttg cgtatcttca ggatgggaaa
     3961 aatcaaagag ctggccccaa ccggttattt cagtgatctc ctcttcaatg ctcagaggga
     4021 tttccatgga gaaatctccc ggataggatg aaacaagttt aaaagcatcc gtaaacattt
     4081 ctttggtggg tgatgtgagc tctccatatt gacctgccag aattatattg atggaaactc
     4141 tttcatatat gttcagcttg ccattttcag accttgaaat aaatttggtg attcgaccat
     4201 catcgatttt tacatttaca gtggtttttt cattattaag tcccatggaa atgaactttt
     4261 ttccacccga taccattatg gtcataccat tattgaattt ctcgattttg gccgttctta
     4321 tccaatcgaa cccgaatttc tgtatcagga attctttgat atttatatct ttttcatccc
     4381 atgaaaatac ttcttttatc gaaataagct taggacagcc taaatttgga cttagtatta
     4441 atggaaattc ttctttttcc gtgttttcca cccatatggc aaatttacta tattgtattt
     4501 atttcttttt gtttggtttg caagatttta tattataggt ttttcatgcc tcaaaaagta
     4561 gaaataccat gaaaaataat tacaaatatg tgggaacatg tcaaaactcg caattatcta
     4621 tggtaccggg tctcataaca cagagaagat ggctgaagct atagaagaag gtgcaagagc
     4681 ggaaggcatt gaaactatat tgaaaaatgt ctcttgtgcc cataagagcg acctcattga
     4741 tgccgatgcc attgctattg gttcgccaaa ttaccgtgat ttgatgatgc catccgtgca
     4801 aaaattcctg aatgaactgg cagatgtaag cctctccggg aaaatcggcc ttgccttcgg
     4861 gtcttttgga tggggtcttg aagccataga ggcgatcaaa gaacagttga tatcatacgg
     4921 tgttgagatg ataaaggatc tttgtataaa aaggatgccc ggggaagagg agcttgagat
     4981 atgcaggagt gttggctcgt ttctggcctg taagatcaat gataaggagg taaaatgtgc
     5041 agcgtaggtg attcgttcgg agtagaactc gaaggcggaa tggaaccgca tttatatgtg
     5101 tgcaatcatt gcagcaagac attcaaaggc attggcacca atttgaaatg cccgaaatgc
     5161 ttttcgaaag atgtcaaatg tattgaatga aactcccttt cggaaggagc ttcccgcttc
     5221 attgacgtag ctggaccaca attaaagaaa tggccacgta ataattttac tacaacagat
     5281 tggggatggt atagaggagg tttaaaacct ttatcttact ataaaaacca atccgtagat
     5341 tatattatta taacgaatgc atatatatat acagatatgt attataaaga gcctggcaaa
     5401 tatgcgaaag aaatctcaaa ctataatgaa ttattaaatg gggccgaatt gattaaggta
     5461 tttgataata aagataatcc aggacctata atcgagatat atagatttag ttctatttgg
     5521 tgaaatttca aacacatgag aatatatagg agttagataa aatggcaaag gtacagagtt
     5581 tttttttaac caaacctcac cttgcgccac cgccgccacc tccaaatcca cctccgccac
     5641 cgaatccgtc acccccactg aatccgccgc ttccggatga tgggggagag ggcggagacg
     5701 tcataccata tgcacctctg aagtatatcg gcatgtaagt cactgatcgg gcttcaggaa
     5761 tctgtatgtt aagttctttc atggcctcaa caaccttatc tcctacaccg agcgttgttg
     5821 catagacaag ccattcctgc catatcttca ggtcatcagg tgcatatttc tttattgatg
     5881 caaaatccgt caggaatgtc ttgaaagcat cccactcgag tttttcttta tagtaattct
     5941 ctttccacct tccgaaaagc gaagatgaaa caaataacgg gggaatcgtt tgcgctaaaa
     6001 ggaccaggga ggtatatatc ccatacgaaa ataaaaagga agttacaagc aggacaaata
     6061 gaaaaagtcc gaagatcatc catgtatatt ttttcccgct cttgacaaat tcttctgcag
     6121 cctttttctc agggactctc attacattat tcatcgattt gcgaatatcg ctcagttcgt
     6181 cctgggcact cgaactccct cctgtgatcg aattcctcaa ttcttcgatc tcgtcttcaa
     6241 atttactgct ttcaaacaca ttatctgatg agtactcctc caggaacttg agcaccttta
     6301 tctcatattc gtcttctcca gcaacatgtc ctttcatgag agttatcctt aatccctcgt
     6361 cctccactac tttaaaaccc agcattttta tctttaattt cttttctccc ccggtttcaa
     6421 ttttgataat atctcgcttg tgcaggtcaa gaagcgttgc ataaaatcca ttctcatcat
     6481 aatcaaatgg atcttttctg aaaacaagat tcacaagcca tggtttcctg ttcctgggaa
     6541 caaaactcag gaattttggc acaatgaaac ttttctccct gccgaatctg taataaacaa
     6601 atgctagcat aaccgggaac aggaacacca ggactttcaa tgcaaaactt aattgtgaaa
     6661 aaaaagaata tttggaattt gcctgaatcg ttttttcttt gacccctgac attgaatttg
     6721 gaaaaccatc catttccgtt gaaattgcag gatcaagcag catctcgatc tcaaaaagtg
     6781 aatccttccg gcttgctcct cttacaatcc aggtatcacc ctccttcttt acatccatta
     6841 aagaatgttc gaaaagttga gaaacataac cctgagggtc atgaacgact attatgactt
     6901 tattgtaagg caggtgctca tctgcaagtt ttaaattcag atgg
//
LOCUS       DATGBN010000179         8077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_169393, whole genome shotgun sequence.
ACCESSION   DATGBN010000179 DATGBN010000000
VERSION     DATGBN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169393"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VKL21_04250"
     CDS             <1..449
                     /locus_tag="VKL21_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45015.1"
                     /translation="LHENINKDSVSKENQTRILYLITIFYGYRIKFITEDGGMILLYS
                     SKDEKEVDQAYRQVENYLQWEGDKTPRRVSYLQNIFMSNDKPGSPYFFTAFSDDLSND
                     QTLQELRDSLGQWLNKENVQNADNALQTFIRTFKSSIDKLYSAWET"
     gene            1335..2798
                     /locus_tag="VKL21_04255"
     CDS             1335..2798
                     /locus_tag="VKL21_04255"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMB45016.1"
                     /translation="MTLTKDINLTNNEKRVLLALSKKDKFSPEDLAAVSELNIEASMQ
                     SAFMLAEKNLCEVKETITTIYNLTKEGEQYAQISLPERQVMDSIKAPLLLSDLKKKFP
                     PQMVGIALGWLRKKNWVKIEGDRIIPLEKAEVGEDETILSKLKKGPLSDIGEAVRDLI
                     KRNLVEKTEKKSRIIIITAEGKALAAGSLTIEEEIVQLTPTIITSGSWKNAHMRPYDI
                     HTRIKPIYGAKLHPYRRLINEMRQIFLEMGFTEIKGDIVQSSFWNFDALFQPQEHPAR
                     EMQDTFHLSTECDLPEEYIKSVKEMHENGGGISRGWGGKWSEDVARKQVLRTHTTAVT
                     IKYLADHPDTPVKAFCIDRAYRREAIDPTHTPEFEQLEGVVMDKDVSFKNLLGCLTEF
                     YHRMGFDEVRFRPGYFPYTEPSVEPEVYIESRGKWVELGGAGVFRKEVTLPLGIKQPV
                     LAWGLGVSRVAMLRLGLKDLRELYQSDIDWLRKSTVI"
     gene            complement(2888..3547)
                     /locus_tag="VKL21_04260"
     CDS             complement(2888..3547)
                     /locus_tag="VKL21_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HMB45017.1"
                     /translation="MNNFEYIPPKNTPVSTEELINDLKKVASENNIDVLPQRLYAEKG
                     KYDVTNISRRFGTWRKALLKAGLKPGNINNYSNEELFENILNIWQYKGKQPTRRDLIC
                     KPSKISQYPYNRRFKSWSNALLSFVKYANEKDNIEFKFNSSQCSIQKTNRDPSLRLRF
                     KVLKRDNFTCVQCGASPAKVEGVELHVDHIKPCSKGGETIYENLQILCLECTLGKSNR
                     E"
     gene            complement(3552..3836)
                     /locus_tag="VKL21_04265"
     CDS             complement(3552..3836)
                     /locus_tag="VKL21_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45018.1"
                     /translation="MKITRKDVARKIADYLHHQITLQELVDWAENMMMEADFESHDLE
                     ALRDVVSRLGLADVKAFGMTWEDCEYLLSRLGYLARIVVTDGSAMAEVES"
     gene            complement(3833..4090)
                     /locus_tag="VKL21_04270"
     CDS             complement(3833..4090)
                     /locus_tag="VKL21_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45019.1"
                     /translation="MDELKRKINERKFGSWEDLIGGGRRYSYKVEGRHGWTACYVKEV
                     NQMEETLRFCQEIYDGNGNLVEIHEKYPDDNGHKKIIEDKP"
     gene            complement(4248..5039)
                     /locus_tag="VKL21_04275"
     CDS             complement(4248..5039)
                     /locus_tag="VKL21_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB45020.1"
                     /translation="MKVIPILNEPALVVNNTIKALVIADVHLGIEWELYHRGFSIPSQ
                     VEKRKKRICDYLEIVNPDRIVLLGDIKHNVPRTSWQEKKEVPDFLETIAAYAPVDIVP
                     GNHDGDIEELINEKVTLHGMRGFVLDGTGYFHGHTWPDTELLSAKNVVMSHNHPTVRL
                     TDSLGHAVSEEVWIRTRFIEDAIREHYGDNIDWKDPDVIIMPAFNELCGGIPFNESIR
                     EELLGPVFANHAIELEGAVAYLLDGTNLGTIGKIRKLGITKRRRG"
     gene            5169..5660
                     /locus_tag="VKL21_04280"
     CDS             5169..5660
                     /locus_tag="VKL21_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45021.1"
                     /translation="MKSNLFKFTILLAVIIVAGSGCIKTPVKMPPINNSQILAVEIGD
                     KNSKQDVNAEWEILEEEYPIYRGIDFYRNAGYGVTKVTFEYSPTSGMGTLFIKKNEDD
                     WGNKSKANYHISGMRLLFKKGSGEIYDTGMILLQKDIWTKSISVSKEKFSGFKVEEIE
                     ISP"
     gene            complement(5661..6632)
                     /locus_tag="VKL21_04285"
     CDS             complement(5661..6632)
                     /locus_tag="VKL21_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45022.1"
                     /translation="MEKIEKIMDREIAWRIFAHEFNMSNLFVSEGDERAPNYIITPTG
                     VKCNRLFIIGVITEVENIGKDNNLWRARIADPTGVFTIYAGQYQPEAAIFLSELLVPS
                     YVALVGKARKYEPEDGAVYLSVRPEEMNISDEKQRDRWVLEAAERTLERINIMEDALN
                     SGLSGNELHEFLLTKVSNTALANGAMHAIKHYENLEKTLLEIKKALAHAIETVTIDSG
                     IIISPQKPEEMKVQDQPEKNIDEFKTHERSFEKMKVQANPPELEPKEILASIIDELDT
                     GKGTSFSKVLEISQKAGLDAKTVELSLKELMDEGRCYEPKIGVLRKV"
     gene            complement(6634..7899)
                     /locus_tag="VKL21_04290"
     CDS             complement(6634..7899)
                     /locus_tag="VKL21_04290"
                     /inference="COORDINATES: protein motif:HMM:NF005554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Single-stranded DNA binding protein"
                     /protein_id="HMB45023.1"
                     /translation="MEIEFAPHVEEIKKALDNEIEENHIIADLKKLLEYRVPLSEAKR
                     SLIKKYGGSEKNTVRKLKDIRIGEHNIEIIGQVVEVIKKTIKIKNIEKILFSGVICDE
                     TSSRSFTAWSDFDLNPGDVISVTQAYVRNWQERPEVNFGPRSKVTKLTTKIQVPEDFE
                     LKKLAELRDGDINVHTQFTIINIEKIDINSKDGSKKILGGIVADEDTKLPITAWVLLP
                     ELAAGNAIEVKNAYVRSFRGVPTLNITESSMVSKLERKLEYSEKEGLKIGDVIIKDGA
                     YDIVVEGNILSIRPGSGLIARCPECSRVVQKGICRVHGKVDEKNDLRIKAIIDDGTGA
                     LTLVLDSRLTLKICEITIEEAQKITQTAMSPKAVEDEIKKKIIGRRFQARGNMSKGEF
                     GITFVAVDAWEPAGSIREQAQRLLGRIAD"
BASE COUNT         2328 a         1734 c         1481 g         2534 t
ORIGIN      
        1 ttttacatga aaacataaat aaggacagcg tgagcaaaga aaaccaaact cgaatactgt
       61 acctcatcac aatattttac ggttatagga taaaatttat aaccgaggat ggtggtatga
      121 tacttcttta ctctagcaaa gatgagaagg aagttgacca agcctatagg caagttgaga
      181 actatttgca gtgggagggt gacaaaacac ctcgaagagt ttcttatctc cagaatatat
      241 ttatgtcaaa tgataaacca ggtagtccat attttttcac agcattttca gatgacttat
      301 ctaatgacca aactctgcaa gagctaagag attcactcgg acaatggttg aataaggaaa
      361 atgtccaaaa tgctgataat gccttgcaga cgttcattag gacatttaaa tcgagtattg
      421 ataaactcta ttcagcgtgg gaaacatgat ctatgaacgt tcacaccaga cgtatcatct
      481 catttaaacc tgccaggcct ccttttcgtc agcagtttca ttttcccaag gcaaaggcgt
      541 aacatcaaaa acgagaggtt tcatcgcagc gcctttttaa tgtccctgct tataaattcc
      601 tcagacctga tccagacatc gcattggtaa gacaaaaaaa aatcggaaaa taaacgacct
      661 tacagcgttt gcagaacaat tgggtgtcac agcacgtgga cttagaaaac gataagaaca
      721 tcaacattgc ttcccccatg tttcttctcc ccgggcatag gaaccaaaga tccttttaac
      781 ttttgcttta tacttctact ggacttcgtt attagcctgt ttcaggattt ttataatatc
      841 ttcaattttc atatatgcac ctttattcaa tgatcaaaaa acaaacccca tttttcctga
      901 tcatcgctgt tggcagccat ccatttgtcg tcacctgcgt atttgtctga aaatatttta
      961 cgaataattc ctatatgttg aatcgtattt aaaaattcgc ttttgcatat ctgaaaaacg
     1021 atctaaccaa ccggagttcg taacagttcg tattaagttc gttgtttttt attaattcca
     1081 tcatatccct ctatccaaga taaacaacga actaaaacga actgaacaaa ctctttgatc
     1141 atagatttcc acttcaaacc agtattattt ctttttcaat ttaaatcgaa agctacagaa
     1201 gtatacatat atcctgcgaa ttgatacctt gctcttcaat tcatttcaag ggtagaaact
     1261 tacctgcttt acataatagt tgatttcttt tcaaaaatga ttaaatatcc tactgttcat
     1321 tctaatgcca tattatgacg cttacaaaag acatcaatct cacaaacaat gaaaagcggg
     1381 ttttgctggc gctttcaaaa aaggacaaat tttcacctga agaccttgct gcggtctcgg
     1441 aattaaatat tgaagcttcc atgcaatccg cattcatgct ggctgaaaaa aacctgtgtg
     1501 aagtaaaaga aactatcacg accatataca accttacaaa agaaggggaa cagtatgcgc
     1561 aaatatcact tcctgagcgg caggtcatgg attcgataaa agcgcctctc ttgctttcag
     1621 atctgaaaaa gaaattcccg ccgcaaatgg tggggatcgc ccttggctgg cttcgaaaga
     1681 aaaactgggt gaaaatagaa ggcgacagga tcatacccct ggagaaagca gaagtagggg
     1741 aagatgagac aatactttca aaactcaaga aaggtccgct atcagatatc ggggaggctg
     1801 tcagggacct gataaaaagg aacctggtcg aaaaaacaga gaaaaagagc cggatcataa
     1861 ttataacagc agaaggaaaa gcccttgcag cagggagttt gaccatcgag gaggaaatag
     1921 tgcagctaac gcccactata atcacaagcg gttcatggaa aaatgcgcac atgcgccctt
     1981 atgatatcca tacccggata aaaccgatat acggcgcaaa actccatccc tatcggcgct
     2041 taataaatga gatgcggcaa attttccttg agatgggctt taccgagata aaaggggaca
     2101 tcgtccagag ctcattctgg aattttgatg cccttttcca gccgcaggag catcccgcca
     2161 gggagatgca ggatactttt cacctgtcca ccgaatgcga tctgcctgaa gaatatatta
     2221 aatcggtgaa ggaaatgcat gaaaacggcg gcggcatttc ccgtggatgg ggaggcaaat
     2281 ggagtgagga tgtcgcccgc aaacaggtgc ttcggaccca cacgactgcg gtcaccataa
     2341 aatacctcgc agatcatccc gacacgcccg tcaaagcttt ctgcatcgac agggcgtaca
     2401 ggcgcgaagc cattgacccc acccataccc ctgagttcga gcagctggaa ggtgtggtga
     2461 tggataagga cgtgagcttc aagaaccttc ttggctgcct cacagagttc tatcacagga
     2521 tgggttttga cgaagttcgt ttcaggccgg gatattttcc gtacactgaa ccaagcgttg
     2581 agcctgaggt atatatcgaa agcagaggaa agtgggtcga acttggcggc gccggtgtat
     2641 tcaggaaaga agtcaccctt ccgcttggca taaaacaacc tgtgcttgca tgggggctgg
     2701 gagtaagccg cgttgcgatg ctgcgccttg gacttaagga tctgagggaa ttataccaga
     2761 gcgatatcga ctggctgagg aagagtactg tgatttgagt atgatgcact tgtcctgaga
     2821 taaggtagct taaccgacgg ttcactttaa ggaggaagtc caatattttt agataagatc
     2881 attaaattta ttccctatta cttttcccaa gggtgcattc caaacatagt atttgtaaat
     2941 tttcatagat tgtttcacca cctttgctac aaggctttat gtgatcaacg tgtagttcaa
     3001 caccttcaac tttggctgga cttgcaccgc attgaacaca agtgaaatta tcccttttta
     3061 gaactttaaa tctcaatctc agagaaggat ctctattagt tttctgaata gaacattgtg
     3121 atgaattgaa tttaaattca atattatcct tttcatttgc atattttaca aaagataata
     3181 gtgcattcga ccacgatttg aaacgtctat tgtatggata ttgagaaatt ttagaaggtt
     3241 tacaaattag atctcttcta gttggttgtt tacctttata ttgccagatg tttaatatgt
     3301 tttcaaataa ttcttcgttt gaataattat taatatttcc cggtttcaaa cctgctttta
     3361 ataatgcttt tctccatgtt ccaaatcgtc tcgaaatatt agtaacgtcg tattttcctt
     3421 tttctgcata aagccgctga ggtaaaacat caatattatt ttcagatgca acctttttca
     3481 aatcgtttat taattcttct gttgaaacag gtgtattttt gggtggtata tattcaaaat
     3541 tattcatatg tttagctttc tacttctgcc atcgctgaac catctgttac tactatcctt
     3601 gccagatatc caagtcgtga taataagtat tcacaatctt cccatgtcat tccaaatgct
     3661 ttcacatcag ccaggccaag tctgctaaca acatccctaa gagcttcaag atcatgactt
     3721 tcgaaatctg cttccatcat catattttct gcccaatcaa caagctcctg tagtgtgatt
     3781 tgatgatgca aataatctgc tatttttcga gctacatctt ttcgagttat tttcatggtt
     3841 tgtcctctat tatcttttta tgtccattat catcgggata tttttcatgg atttcaacca
     3901 ggttgccatt accatcatag atttcttggc aaaaccttaa agtttcctcc atctgattta
     3961 cttcctttac ataacaagct gtccatccgt gacgaccttc gactttatag gaataacgcc
     4021 gtccaccacc tattaaatcc tcccacgatc caaattttcg ttcatttatc ttgcgtttaa
     4081 gttcatccat gtttatttct tcaatcctga tattgaatca ttaaaattat atgtttcttc
     4141 atctcgatcc catttatatt gtttcataaa aacctgtaag tacttaccat tttatatttg
     4201 aaatagtaag taaaattatt tcagatggaa agtttcagca aaaaaagtta ccctctcctc
     4261 cttttcgtta ttcccagctt cctgattttt cctatcgttc caaggtttgt accatcaagc
     4321 aaataggcca ctgcaccttc aagctcgatg gcgtggttgg caaatacagg accaagaagt
     4381 tcttcacgta tggactcatt aaatggtatc cctccacaca attcattaaa cgcaggcatt
     4441 ataataacat caggatcctt ccaatcaata ttatccccat aatgctctct tattgcgtcc
     4501 tcaataaaac gcgttcttat ccatacctct tctgatactg catgcccgag ggaatccgtg
     4561 agccttaccg ttgggtggtt gtgggacatc accacattct ttgccgataa taattccgtg
     4621 tcaggccagg tatgaccgtg aaaataacct gtgccgtcaa gcacaaagcc ccgcattccg
     4681 tgcagcgtga ccttttcatt aataagctcc tcaatgtccc catcatgatt ccccggcaca
     4741 atatcaacag gcgcataagc tgcgattgtt tcaagaaaat cgggcacctc ttttttttcc
     4801 tgccatgatg tcctgggtac attgtgttta atatcaccaa ggagcacgat ccggtcggga
     4861 ttcacgattt caagataatc acatatacgc ttcttccttt tttcaacctg gctggggatg
     4921 gaaaaacccc tgtgataaag ctcccattcg ataccaaggt gaacatccgc gattacaagg
     4981 gcttttatag tgttattgac aacaagcgcg ggttcattca agatagggat tactttcatt
     5041 tatttacttt tacttcatga atagaaagta taaaagagta tcaatatcca tttgagattt
     5101 aatattaata gttttatcct attaatttat taaggttaaa gtaaatataa ttcacatgtg
     5161 attccgaaat gaaatctaat ttgtttaagt tcactattct tctggcagta attatcgtag
     5221 ctggcagcgg ttgtataaag acgccggtaa agatgccgcc aatcaataat tcgcagattt
     5281 tggcagtaga aatcggggat aaaaatagca aacaagatgt aaatgcagaa tgggaaattc
     5341 tggaagaaga atatcctata tacagaggga tcgattttta tagaaatgca ggatacgggg
     5401 taacaaaagt tacctttgaa tattcgccta cttcaggtat gggaacgtta tttattaaaa
     5461 aaaatgaaga tgattggggg aataaaagca aagctaacta tcatattagc ggtatgagac
     5521 ttctttttaa gaaaggatcc ggcgagatat atgataccgg gatgattctt cttcagaaag
     5581 acatctggac aaagagtatc tctgtgtcta aagagaaatt ttcaggattc aaagtggaag
     5641 agatagaaat ttcaccctga tcagaccttc ctgagcactc cgatctttgg ttcatagcat
     5701 ctcccttcat ccataagctc tttcagggat aattccactg ttttggcatc caaccctgct
     5761 ttctgtgaaa tttcaaggac tttggaaaag gatgtgccct ttccagtatc aagttcatct
     5821 atgatagatg caagtatctc ctttggctcc aattcaggcg gatttgcctg aaccttcatt
     5881 ttttcaaatg atcgttcatg ggttttgaat tcatctatat ttttttcggg ttggtcctga
     5941 actttcattt cttccggttt ttgaggtgaa attataattc cgctatcgat agtaaccgtc
     6001 tcaattgcat gggcaagtgc ttttttaatt tcgagtaagg ttttctccag attctcataa
     6061 tgctttattg catgcatcgc gccatttgca agagcagtgt tcgaaacttt cgttaacagg
     6121 aattcatgta attcgttccc tgatagccct gaattcaatg catcttccat aatattgatg
     6181 cgctctagag ttctttctgc tgcttccagt acccatcggt ccctttgctt ttcatcagaa
     6241 atattcattt cttccggcct tactgataaa tacaccgccc cgtcttcagg ttcatatttt
     6301 cttgctttgc ctacaagtgc aacataagaa gggaccagca attcagataa aaatatggca
     6361 gcttccggct ggtactgccc tgcatatatt gtaaataccc ctgtagggtc agctatgcgc
     6421 gcccgccaga ggttattatc ctttccgata ttttcaactt cagtgataac cccgataata
     6481 aaaagccggt tgcattttac gccagttggc gtaattatat aattgggggc tctttcatca
     6541 ccttcactga caaataagtt tgacatattg aattcatggg caaaaatacg ccatgctatt
     6601 tcacggtcca ttattttttc gatcttttcc atattaatcc gctatcctcc caagaagccg
     6661 ttgtgcctgc tctcgtatcg atccggcagg ttcccaggca tctactgcca caaatgtgat
     6721 cccgaattca ccttttgaca tatttcctcg cgcctggaac cttctcccga taattttctt
     6781 tttaatttca tcttcaactg ctttcgggga cattgctgtt tgtgtgattt tttgagcttc
     6841 ttcgatggta atttcacata tcttcagggt aagcctggaa tcaagaacta gcgtcagtgc
     6901 accggttcca tcatcaatta tagccttgat cctcagatca ttcttctcat cgacctttcc
     6961 atgcaccctg cagattcctt tctggacaac ccgtgaacat tcggggcacc tggctatcaa
     7021 tcccgaacca ggcctgattg aaaggatatt cccttctacc acaatatcat aagccccatc
     7081 tttaattata acatccccga tcttcaatcc ttccttttcg ctgtattcaa gcttcctttc
     7141 aagctttgaa accatacttg attcagtgat gttcaaggtg ggaacacccc tgaaggacct
     7201 tacataggca ttttttactt caatcgcatt acccgcggca agttcaggta acaataccca
     7261 tgctgttatc ggaagttttg tatcctcatc tgcaacaatt ccaccaagta tttttttgga
     7321 accatctttt gaatttatat caattttctc gatattgatg atagtgaact gtgtatgaac
     7381 attgatgtca ccatctctta attctgcaag cttcttaagt tcaaaatcct ctggcacctg
     7441 gatttttgta gtaagctttg tgacttttga gcggggccca aaatttacct caggccgttc
     7501 ctgccagttc ctcacataag cctgtgtgac tgaaattaca tccccgggat taagatcaaa
     7561 atctgaccat gcggtgaaac ttcttgatga ggtctcatca cagatcactc ctgaaaaaag
     7621 gatcttttcg atattcttga tcttgatggt tttttttatt acctcaacga cctgtccgat
     7681 tatctcgata ttgtgctcac ctatccggat gtctttaagt ttcctgactg tgtttttttc
     7741 agacccgccg tattttttta tcaggcttcg ttttgcttcg ctcaatggta ctctgtattc
     7801 gagcagtttt tttaaatccg caatgatgtg gttctcttct atttcgttat caagcgcctt
     7861 ttttatttct tcaacatgag gcgcaaattc aatctccata aagcctcctt ctgactaatg
     7921 ggatacgagt tataggataa taaaagataa aaaaccatcg gatcaaaatg caattatttg
     7981 ttctcgatac taaagtccaa aagattatgc gctgttttat tactgcaaga ggatcatgag
     8041 atatttttat aaaccataaa gagtattatg attataa
//
LOCUS       DATGBN010000180         6144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_441235, whole genome shotgun sequence.
ACCESSION   DATGBN010000180 DATGBN010000000
VERSION     DATGBN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441235"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..339
                     /locus_tag="VKL21_04295"
     CDS             142..339
                     /locus_tag="VKL21_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45024.1"
                     /translation="MKIKKCCYLDRDCIPACVAYSTAKELSENAKQMGLNDMHCMRLI
                     TELASLVNLTNSEHLENEEEF"
     gene            complement(336..1118)
                     /locus_tag="VKL21_04300"
     CDS             complement(336..1118)
                     /locus_tag="VKL21_04300"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMB45025.1"
                     /translation="MKKMSYPEIKDKIVAFISSRVCGSGAKGAVIGLSGGIDSALTAY
                     LTVLALGKENVLGLLLPEKGISSKADIDDAIEVANILGIEYKIIEISPIMASFSSIIP
                     VFDNESKTANGNLKARTRMCILYYHANLMGRMVIGTGNKTELLLGYFTKYGDGGVDIE
                     PIGDLYKTGVRGLSGYMGVPDRIIEKTPTAGLWPGQTDENELGVSYETADQVLTLLVD
                     EKKNISEIKRIFPPEIVDKLAARISSSGHKRMPPCAIEIGFS"
     gene            1249..2184
                     /locus_tag="VKL21_04305"
     CDS             1249..2184
                     /locus_tag="VKL21_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195338.1"
                     /note="Replication protein A protects and stabilize the
                     intermediate ssDNA that is generated by the unwinding
                     action of a DNA helicase at the replication fork. In
                     addition, SSBs prevent the formation of secondary
                     structures by single-stranded template DNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor A"
                     /protein_id="HMB45026.1"
                     /translation="MSEITKQIEARFLELGVKVETAEIDIMLDQLINKFKVPAEEARR
                     NVTNHFLKKHNIPKTEFFTPRAAPQVKASDIKEDGKWVTLRCKVVQLWETNHESLSQT
                     GLLGDESGTVAFKKWTKANLAQVEEGKNYIFKNVATSLWQGRFSVSLNKTSEIIPITE
                     DIKVGKTLVEFHGAIVDIQSGSGLIKRCPECNRALIKGACGEHGKVDGIYDLRVKAVM
                     DDGEKVQDILMNREVTEAFTGITLDKAKEMATEALDQAVVLDVIKSRLVGRYYVVIGA
                     RLDRFILVDTIKQDVKPIEEDIKRILASTAVAVEA"
     gene            2187..2762
                     /locus_tag="VKL21_04310"
     CDS             2187..2762
                     /locus_tag="VKL21_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMB45027.1"
                     /translation="MAESASGFVREIAHRIFAAELKESNLQAKEGQDQYSPQYLITPT
                     GAKANRVFIVGTLTEKEDIGTDSEFWRGRIVDPTGAFFVSAGQYQPEAAQVLAKSAPP
                     EFIAVIGKPTTFTTKEGNVLVSIRAESMHVVDGATRDRWVVETADLTTKRLSKLNGNE
                     PDAVKAKEHYSTNVEQYKAMVAMALESMKVR"
     gene            complement(3452..4144)
                     /locus_tag="VKL21_04315"
     CDS             complement(3452..4144)
                     /locus_tag="VKL21_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HMB45028.1"
                     /translation="MRGTLYTVFPLPFTSVVSEIASSPFEATIILNQSIVLGVNSSTL
                     SRKSSYANKKLVGNIYYIKIYVEVKEMNKQYRIIFAVSVIALFVVGVGVAIPNPASKY
                     CVEQGYNNTIRTNPDGSQTGYCIFPNGTECEEWSFFRGECKFGATNLTNNITDVNETN
                     ENETNATEIAIEAGNATGMPVNTTVVPATPSGTTSEPTIPVSTTKPISGFGIVMATVV
                     VISTIHIFGRRR"
     gene            4282..4695
                     /locus_tag="VKL21_04320"
     CDS             4282..4695
                     /locus_tag="VKL21_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMB45029.1"
                     /translation="MIKTIDHIGIMTKDLQQSVDFYIDVLGFSISAKIDMENIGFSAI
                     FVEKNGSKIELMGYRGEIPKRSESIEIKLGGGSIPINDHITFSVDDIGATVTELKEKG
                     VEFGLEPTQLEGGMKLASFKDPNGVLIELVEHPKK"
     gene            4814..5443
                     /locus_tag="VKL21_04325"
     CDS             4814..5443
                     /locus_tag="VKL21_04325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB45030.1"
                     /translation="MNISMKEHWDEIYEALDVDELTWYEEMPEPSIKLLSKSHITKDE
                     TILDVGAGASTFVDYLINQGFKNIIAADISKIALNKLKERLGKEKTSSVKWIVDDITQ
                     PIHIQNLRDIAVWHDRAVLHFLLEERQQQMYLSTLKKVIKKGGYVIIAAFSLKGAKKC
                     SGLDVKNYDQNMLAKFLGEDFRLLEYFYYTFYMPSGKMRPYIYALFQKS"
     gene            complement(5459..5755)
                     /locus_tag="VKL21_04330"
                     /pseudo
     CDS             complement(5459..5755)
                     /locus_tag="VKL21_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
     gene            complement(5841..>6144)
                     /locus_tag="VKL21_04335"
     CDS             complement(5841..>6144)
                     /locus_tag="VKL21_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMB45031.1"
                     /translation="IKLMNPIAEALTGWKQNEALGKPLANIFNIISENIDEKIEEPIT
                     KAIRNDRFYGLANNTVLVTKEGMKRYVDVIGSTIKIDGNKIIGFVFIFDDINWRIR"
BASE COUNT         1935 a         1204 c         1287 g         1718 t
ORIGIN      
        1 tgattaacgg gagcactaca cagaatgccc ccgatgcatg gtcaacagtg acaactttgc
       61 aatatgcagt agaatactca caaccaaata taccaaaaag aaagaaagga ttttatatgt
      121 cctgatatta ataaccggat aatgaagata aaaaaatgct gttatctgga tagggattgc
      181 ataccagcgt gtgttgcata tagtactgct aaagagttaa gcgaaaatgc aaaacaaatg
      241 ggtttgaacg atatgcattg catgcgtctt attactgagc ttgccagctt agtgaatttg
      301 actaactctg aacatctgga aaatgaagaa gaattttagg aaaatcctat ttcaatcgca
      361 caggggggca ttcttttgtg cccgcttgat gagatgcgcg ctgccagttt atcaactatt
      421 tcaggaggaa atatgcgttt tatttcggaa atattttttt tttcatcaac aagcaaggtc
      481 aatacctggt cagccgtctc atacgataca ccaagctcgt tttcatctgt ctgcccgggc
      541 cataatcctg ctgtaggtgt tttttctatg atcctgtcag gaactcccat gtatccggaa
      601 agtccgcgca cccccgtctt ataaagatcc ccgatcggct cgatgtcaac cccaccgtcg
      661 ccgtattttg tgaaatagcc cagaagcaat tcggtcttat ttcctgtccc gatcaccatg
      721 cgacccatga ggttggcatg atagtacaga atgcacatac gcgttctcgc tttcaggttg
      781 ccgttagcgg tctttgactc attgtcaaaa accggaataa tagatgaaaa tgaagccatg
      841 ataggagata tttcaattat tttatattca ataccaagta tatttgcaac ttcaatggca
      901 tcatcaatat cagctttcga tgatatgcct ttttccggga gcagcaaacc aaggacattt
      961 tccttgccca gggcaagaac cgtgaggtat gcggtcaatg cggaatctat acctccgctt
     1021 aaaccgatga cagccccttt tgcccctgat ccgcagaccc gggagcttat gaaagcaaca
     1081 attttatctt ttatttcagg ataactcatt tttttcatct ctgcttaatg aaattccagc
     1141 ttattaaaat attactttca caccgctccg ggaagcttta tatattcaca ggataaggta
     1201 aagttgcctc tcaaacacaa aaatcaaaca ggaggcttga ggaaatatat gagtgaaata
     1261 acaaagcaga tagaagccag gttcctggaa cttggtgtta aggtggagac agctgaaata
     1321 gatataatgc ttgatcaatt gatcaacaag ttcaaggttc cggctgaaga agcccgtcga
     1381 aacgtaacaa atcacttttt aaagaaacat aatattccga aaactgaatt tttcacgccg
     1441 cgggcagcgc cacaagtaaa agcgtcagac ataaaagagg atggtaagtg ggtaactctt
     1501 cgctgcaaag tagtccagtt gtgggagaca aatcatgaat ccctttccca gaccggattg
     1561 ctgggtgatg aatcaggaac agtagcattc aagaaatgga ctaaagcaaa ccttgcgcag
     1621 gtcgaggaag gaaaaaatta tatttttaag aatgtggcaa cttcgctatg gcaggggcgg
     1681 ttcagtgtca gcctgaacaa gaccagtgaa atcatcccga taaccgaaga tatcaaagta
     1741 gggaaaacgc ttgtggaatt ccatggcgca atagtggata tccagagcgg ctcaggtttg
     1801 ataaaacgat gcccggaatg taacagggct ctaataaaag gcgcatgcgg cgaacatggg
     1861 aaggtggatg ggatatatga cctgagggtc aaagccgtaa tggatgacgg ggaaaaagtg
     1921 caggatatat tgatgaacag ggaagtgacg gaagccttca cagggatcac ccttgataaa
     1981 gcaaaggaaa tggcaacaga agcgcttgac caggcggtgg ttcttgatgt aataaaaagc
     2041 aggctcgtgg gacgatatta tgtcgtgatc ggagcaaggc ttgacaggtt cattctcgtt
     2101 gataccatca agcaggatgt aaagcctatt gaagaagaca taaagaggat acttgcatca
     2161 actgcggttg cagtggaggc gtaaccatgg ctgaaagcgc atcgggattt gtcagggaaa
     2221 tagctcaccg catatttgcg gcagagttga aagaatcaaa cctccaggca aaggaaggac
     2281 aggaccagta ttcaccgcaa tatcttatca cacccacagg cgcaaaagca aatcgcgtct
     2341 tcatcgtggg cacgctcaca gaaaaagaag acatcgggac tgattccgaa ttctggcgcg
     2401 ggcgcatcgt tgaccccaca ggcgcattct tcgtgtcagc cgggcagtac cagcccgaag
     2461 cagcgcaggt gcttgcaaag tccgcacccc cggagttcat agccgtcata ggaaaaccca
     2521 cgaccttcac gacaaaggaa ggaaatgtcc ttgtatccat cagggctgaa tccatgcatg
     2581 tcgtggatgg cgccacgcgg gacagatggg tggttgagac cgcggacctg accacaaaga
     2641 gactttcgaa attaaatggt aatgaacctg atgcggtaaa ggcaaaggag cattattcta
     2701 cgaatgtgga gcagtataaa gcgatggtgg caatggcgct ggagtcgatg aaggtgcggt
     2761 agtgccttca tttttttaac tcttaatctg ttttttatat gaacgaaggt aatatatatt
     2821 tataaactct tttggattct aaatatagat aatgttcaac cctaacttca actagccaaa
     2881 atgggaaata tcactcagga gagacgcact tataagggca gcgcatgcat ctactgcaat
     2941 cgaaggaaat ccacttacat tagaagaggt aagccagctt gcgcagggca gaaaaatcat
     3001 ggccacaaga aaagcacagc aggaagtgct taattatcta agggttttag aggacataga
     3061 aaagtatcag gaacatgctg ctttcctccg tcctttccat taccctttga gggaataaat
     3121 ggaacagctc attctcaatt tttgagagtg cgaacttacg ataatactgc ttcccgacat
     3181 tgaattatca aaaaccaaat ttcacgtgaa gttaaggtgt aataattgca tttttactta
     3241 ttcccaacaa gatattccta aagccacctt gtttcactct tttatcccta ttctgactcc
     3301 ccaatatcct cgatatagaa cctctcagct ttcctataga ccgcaattat atccgccaga
     3361 ttgcaggcgc tttttcgaca taggtaaggt ttcaaatgaa tctctgaagt atggaaaacg
     3421 ggaatacaat attgagtgag aagattatct cttaccttct ccttccaaat atatggattg
     3481 tcgatattac gaccacagta gccattacta ttccaaatcc agatattggc ttagtagttg
     3541 atactggaat cgtaggttca ctagtcgtcc ctgaaggggt agcaggtaca acagtcgtgt
     3601 ttacaggcat acctgtagca tttcctgctt caattgcgat ttctgtggca tttgtttcgt
     3661 tctcatttgt ttcatttaca tctgtgatat tgttggtcaa atttgtggct ccaaatttgc
     3721 actctcctct aaagaatgac cactcttcac attctgttcc gttggggaaa atgcaatacc
     3781 ctgtctggga accatcagga tttgtccgaa ttgtattatt atatccttgt tcaacgcaat
     3841 acttagaagc gggattaggt attgctactc ctacccccac aacgaataaa gctatcacag
     3901 atactgcaaa tattattcta tattgtttat tcatctcttt aacctccacg tatattttta
     3961 tatagtatat attaccaaca agctttttat ttgcataaga acttttcctt gacagtgttg
     4021 acgaattcac ccctaaaaca atggactggt tcaagataat agttgcttca aatggcgaac
     4081 tggcaatctc agatacgact gatgtaaaag gtaaaggaaa tacagtatac aacgtgcctc
     4141 tcataattct tcttttggtc gataatagta atatggtagc gttagattta tcttaggcaa
     4201 acaatgaaag gtatagactc tgtttgcaag tcaatggaag aaagttaatg cttgaatttg
     4261 aaaaaacgga agaaaaaaac catgattaaa acaattgacc atatcgggat tatgacgaaa
     4321 gacttacagc agtcagtgga tttctatatt gatgtgcttg gcttttccat ttccgccaag
     4381 attgacatgg aaaatatagg tttttctgca atctttgtcg aaaaaaacgg tagcaagata
     4441 gaactcatgg gatatagagg cgaaattcca aagcgttcag agagtattga aattaaattg
     4501 ggtgggggtt cgattcccat aaacgaccac attacttttt ccgtagatga tatcggagcc
     4561 acggtaactg agctcaaaga gaagggagtc gagtttggct tggagcctac acaattggaa
     4621 ggcggaatga aacttgcctc cttcaaagac cctaatggcg tactgattga actggtggag
     4681 catcctaaaa aataaaacaa atgcatataa gatataaaag ctgatgttct ggcagcatac
     4741 cagaaaaaaa gatattttta cgctcaggat tgatatatcg attgttcagc tgaatgcaga
     4801 gggaggataa aaaatgaata tctcaatgaa agaacactgg gatgaaatat atgaggcatt
     4861 agatgtagat gagctgactt ggtacgaaga aatgcctgag ccatctataa aattactatc
     4921 taaatctcat attactaaag atgaaactat attggatgtt ggagcgggtg catcaacatt
     4981 tgttgattat ctcattaatc aaggatttaa aaacatcatt gctgccgaca taagtaaaat
     5041 tgccttaaac aagctaaaag aaagattggg taaagaaaaa acctcatcgg taaaatggat
     5101 tgtagatgac attacccaac caattcatat acagaattta agggatattg cggtgtggca
     5161 tgatagagca gttttacatt tcttattaga agaaagacag cagcaaatgt atttatctac
     5221 gctaaagaaa gtgattaaaa aaggaggcta tgtcattatc gctgcctttt cattaaaggg
     5281 agcaaaaaaa tgtagcggtt tggatgttaa gaattatgac caaaacatgc tggcaaaatt
     5341 tttaggagag gattttcgcc tgcttgaata tttttattat acattttata tgccttcggg
     5401 taagatgaga ccatacatat atgctttatt tcaaaaaagt taagttagcg atgcgtgctc
     5461 atctaaacct ttcatctgct ttcatatcaa gtgctttatc tgctttattt gaaagccaac
     5521 ctatccgctc gacgtttcgg acatcaaatt ctggaacata tggttttata tccaaaagag
     5581 gtgttccatc tgcaatatcc acatcttcga tatggagagt ggctcctttt actcttacaa
     5641 gcctgactac aggtaatcct ataggatttg ggcgtctggg cgctcttgta gaaaataccc
     5701 catgcagtct gtcatccata aatggtataa cctccaaaga ataaccttta gatagatgaa
     5761 aatggtaatc attctaacag gcgactgaaa tataaagatt caacattatt tttcagtctt
     5821 aacaattttc agcatgtatt ctacctgatg cgccagttaa tatcatcaaa gataaacaca
     5881 aaaccaatta ttttatttcc atcaatttta atggtcgaac caataacgtc aacatatctt
     5941 ttcatacctt cttttgttac caatacagta ttgtttgcaa gaccgtagaa cctgtcattt
     6001 cgaattgcct ttgttatcgg ttcttctatt ttttcatcga tattttcact aataatattg
     6061 aaaatgttcg ccagaggctt ccctaaagct tcattctgct tccagccagt cagcgcctct
     6121 gcgatagggt tcataagttt tatt
//
LOCUS       DATGBN010000181         6618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_363738, whole genome shotgun sequence.
ACCESSION   DATGBN010000181 DATGBN010000000
VERSION     DATGBN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363738"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="nifS"
                     /locus_tag="VKL21_04340"
     CDS             complement(<1..1104)
                     /gene="nifS"
                     /locus_tag="VKL21_04340"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HMB45032.1"
                     /translation="MKQVYMDHSATTATDPAVTEAMLPYFSEKYGNPSSLYTIGRQAR
                     KAIEESRQKVADLIGAKKEEIIFTGSGTESDNLAIKGSAFRNRKKGDHIITSSIEHHA
                     VLHTCKYLEKNGFKVTYLPVSKEGIIDPVDVAKAITPQTILISIMHANNEIGTIQPID
                     EIGKIAKEKNITFHTDAVQTTGKIPINVDDLGVSMLSISAHKIYGPKGVGALFMRKGT
                     VIEPQLHGGGHERNIRSSTENVPGIVGFGKACELAKERLPQELNLAALRDSLIKRVLE
                     IKDSYLNGHPIKRLPNNANFRFSYIEGESMILNLDMKGVAASTGSACSSTSLEPSHVL
                     LAIGLRPEESHGSLRFTLGRENTQEDVDHVISIL"
     gene            complement(1294..1650)
                     /locus_tag="VKL21_04345"
     CDS             complement(1294..1650)
                     /locus_tag="VKL21_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMB45033.1"
                     /translation="MHEIRKLIEYAPKNRDILEVAAYYLKNIILLQAFPDANHRTALT
                     AIEMFLEINGRNLDYTPVEAFDFRKELYNSRLMVYKTYEEMSTRVLKEDDNQTENSVF
                     TLCLKFIKAHVKILQF"
     gene            1849..2127
                     /locus_tag="VKL21_04350"
     CDS             1849..2127
                     /locus_tag="VKL21_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMB45034.1"
                     /translation="MNATIDMVTISSKGQIVIPKKILKVLGLKEQDKLILFSKGENIL
                     LKKVKPEMFEKNLRDMLAPIRKEAEAKGLTQREVEIEIRAHRAQKIPK"
     gene            2124..2534
                     /locus_tag="VKL21_04355"
     CDS             2124..2534
                     /locus_tag="VKL21_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMB45035.1"
                     /translation="MIRLVIDTNVFVSALISTRSIPALLLDEAGRKYSLYISKEILDE
                     VEDVISRKKFGFSIQEISSAMEAVLSFSEIINPEIKVDVVKSDPDDNKILECAIACKA
                     SYIVSGDSHLLELREYGSIKIINPKTALELLNNS"
     gene            complement(2706..2779)
                     /locus_tag="VKL21_04360"
     tRNA            complement(2706..2779)
                     /locus_tag="VKL21_04360"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2743..2745),aa:Phe,seq:gaa)
     gene            2939..3817
                     /locus_tag="VKL21_04365"
     CDS             2939..3817
                     /locus_tag="VKL21_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45036.1"
                     /translation="MDELMEKIQKLQSDYFKRRRLLLILDLISIFAIFYAIFIILSAG
                     FFLDKFFNDPRIPTNIILPIIALIIAIIGAFLLHKNDHKIKFNFLVEGKYPELKEKLR
                     TAYDNISESNVIVDSLKSLVLDGLAIVSSSRLIQGSVIVTKIILVIIFISTATFISLN
                     SDKYSIPPDTIANNFKNLTGIGNTDTNGTIDITGSLQNMENVAQNGSGNIFGKPKIAS
                     IEGKNIDLTLYSGQDTGFEVRDTSQASNQFIKSAAFPVDVLGSNVSDGGYSMLMKKTE
                     SEKQLINKYAVERSKI"
     gene            3831..4778
                     /locus_tag="VKL21_04370"
     CDS             3831..4778
                     /locus_tag="VKL21_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMB45037.1"
                     /translation="MSNETYASSTRIFKELLDEIGKVIVGQHEAVEQMMIAILCNSHA
                     LVESNPGLAKTLTISTISKALDLKFNRIQCTPDLMPSDITGTYIIEDIGGKKQFRFEP
                     GPVFANIVLADEINRASPKTQSALLEAMQEKQVTVGNKTYPLDKPFFILATQNPIEME
                     DTYPLPEAQLDRFLLKILMDYPTFEDENEIVNRYTRNIIPTVKSVVSKTEVLELQKLT
                     RDVPIAEDIKIRAIKIVGATRGKSENIEYGASPRASIGLILAAKARALMKGRNYVSKE
                     DIDAMAYPVLRHRVILTFESERKGMTTDQVIADILKNAK"
     gene            4948..5238
                     /locus_tag="VKL21_04375"
     CDS             4948..5238
                     /locus_tag="VKL21_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45038.1"
                     /translation="MTRIARIFTDSCASTVAPVDVAQPSRQLTPCTRMPVAYVDASSR
                     GATLDVWLAPYMEIRVPMHCICVYLGSSAVNTFNELNWKSDLNDRHLLLQRA"
     gene            5207..6067
                     /locus_tag="VKL21_04380"
     CDS             5207..6067
                     /locus_tag="VKL21_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMB45039.1"
                     /translation="MIDTSFFKELDRFSFMVRKRVSTAYSGSRRSILKGRGMEPVSYR
                     EYTQGDDFKTIDWKVYGRTEKLYVKEFEEEKSLTTHILLDTSKSMDFRNKFEYSAMLA
                     LGFAYLVTKDNEKFAIATFGDEIIITKPKRGRRYLSLTIDLLNSAQLNGKTNFDYCLE
                     KYASVIRSRSLVFIISDFMADVDAIRNSIFRLGNNELVLIQVLDPLEKSLDLGGEAKL
                     IDLETDAKMDIYTSPRLREQYQRKLNDHISKIKETCMTVGADFHTVTTDKPVFDAIFE
                     VVSRREVKIK"
     gene            6077..>6618
                     /locus_tag="VKL21_04385"
     CDS             6077..>6618
                     /locus_tag="VKL21_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HMB45040.1"
                     /translation="MALLDSFYANPLQFANEAGLYALLSIIPLIIIYLLRPRAKKIKI
                     PSIMFLLDIEKKKRLNIFRKFLKDPLFLIQLLALIIISLAIAAPFIMANEEAGGGSTV
                     IILDGSASMQADGRFEKAVVEANKFLSAKNTLILAQSLPLMILKEAPASSAKDVLSKI
                     KAKATVVDLSGAIILGRRMLP"
BASE COUNT         2005 a         1334 c         1428 g         1851 t
ORIGIN      
        1 taagatcgaa ataacatgat cgacatcttc ctgtgtattt tcacgtccca gtgtaaacct
       61 gagtgatccg tgtgactcct ctggtctaag cccgatagcc aggagaacat gtgatggctc
      121 aagggatgtg gatgaacagg cggaaccggt tgatgcagcc acacctttca tgtcaaggtt
      181 caatatcatg gattcacctt caatatagct gaacctgaaa tttgcattat ttggaagccg
      241 ttttatggga tggccattaa gatatgaatc cttgatctca agaactcgtt taattagtga
      301 atccctcaac gccgctaaat ttaattcctg ggggagacgc tcctttgcaa gttcacaggc
      361 ttttccgaaa ccaactattc cgggtacatt ttcggtgctg gagcggatgt ttctctcatg
      421 cccgccccca tgcagttgtg gttcgatgac tgtgcctttc ctcataaaaa gagctccaac
      481 accttttggc ccgtatattt tatgtgcgct gatcgataac attgaaacgc caagatcatc
      541 aacatttatg gggatttttc ctgttgtctg aacggcatcg gtatgaaagg taatattttt
      601 ttcttttgct atcttgccaa tttcatctat gggctggata gttccgattt cattatttgc
      661 atgcattatt gagatcagta ttgtttgcgg agttatggct ttagcaacat caaccggatc
      721 tattattcct tctttgctta cagggagata tgtaactttg aaaccatttt tttccaggta
      781 tttgcaggtg tgcaggaccg catgatgttc aatcgagctt gtaatgatgt gatcgccttt
      841 tttccggttc ctgaaggcac ttccttttat cgcaaggtta tcggattctg tgccgctgcc
      901 tgtgaatatg atctcttctt tttttgcacc gatgagatcg gcgacttttt gtcttgattc
      961 ctcaatagct tttcttgcct gtcgtcctat cgtatagagg cttgagggat tgccatattt
     1021 ttcagaaaaa taaggaagca tggcttccgt gaccgcaggg tcagtagcag ttgtggcact
     1081 gtggtccata taaacttgtt tcatagtgat tcatggtatg gacttaaatt ggaatatact
     1141 taacgatatg caggttaaaa gatatataaa atagatttta aaacaaaatg cttatatata
     1201 taccagggtt gtatgtcata tccaactgac agaatcgtgg ggttaagggg tgagcgcaag
     1261 cccccggctt cagctgtggg gatagcgaac cccttagaat tgtaatatct tgacatgtgc
     1321 ctttataaat ttcaagcata atgtaaatac actattctct gtctggttat catcttcttt
     1381 taagactctt gtggacattt cttcatatgt cttatagacc attaatctac tgttatagag
     1441 ttcttttcta aaatcaaaag cttctacagg cgtataatcc aaatttcgac cattgatttc
     1501 cagaaacatc tctattgcag tcaatgctgt tctatggttt gcatcgggga acgcctgaag
     1561 aagtattata ttctttagat aatacgccgc cacctcaagg atatctcgat ttttcggtgc
     1621 atattcgatt agttttctaa tttcatgcat cttgatcgga taatcttcac ttccagaata
     1681 ttctatcagt ggatcttttg aggcacggac agcgagaagc tgatggaggc ggttgtgggg
     1741 aggatgctgg cgggggaaaa aattataatt aaattattag gcatctctca gatgtactcg
     1801 aattaccgct aaaaaggttt atgtcttata agtaagatat aacttactat gaatgctact
     1861 attgatatgg taactatatc ctctaaaggt cagatagtaa ttcccaagaa gatacttaaa
     1921 gtccttggat tgaaagagca ggacaagtta atattattca gtaaaggcga aaacattctt
     1981 ttgaaaaaag ttaaacctga gatgtttgag aaaaatttaa gggacatgtt ggcgccaatt
     2041 cgtaaggaag cagaagcaaa gggattgacg caaagagagg tagaaattga gataagagca
     2101 catagagctc aaaaaatccc gaaatgatac gattagtaat tgatacgaat gtctttgttt
     2161 ctgctttgat atcaaccaga agtattcctg ctctgcttct tgatgaagca gggagaaagt
     2221 actcacttta tatatcaaaa gaaatacttg acgaagttga agacgttatt tcaagaaaga
     2281 aattcggatt ctctatacaa gaaataagtt cagctatgga agcggtcctg tcgttctctg
     2341 aaatcataaa tccagagatc aaagtagatg ttgtaaaatc agaccctgat gataataaaa
     2401 tactggaatg tgcgattgca tgcaaggcgt catatattgt ttcaggtgat tctcacttgc
     2461 ttgaactgag agaatatggt agtatcaaaa taataaatcc aaagacagca cttgaattat
     2521 tgaataactc ataaaaataa accgggtcat atccctctac gataatcttg aagccacaca
     2581 catgcgccca caggcggaca gcgagaagtc gatggaggcg gtgcaggatg taaacattgg
     2641 aaacctcttg atagttctct ctttaatgaa attaagaaac aggccagtga gcgcttttta
     2701 tagagtgccc agacccggac ttgaaccggg gacatctgcg tcttcagcgc agcgctctcc
     2761 caggctgagc tatctgggca catcacgtgc taatgccgac attgtatatc taacctttgc
     2821 cagcgacgct cacaatatgg ttaaccatag aaaaaggttt cgattattgg catgatacaa
     2881 aaaaacatcc ccaagactta atataattaa aaccaataat gcgggtgaag gtgactgaat
     2941 ggacgaactt atggagaaaa ttcaaaaact ccagtctgat tattttaaac gacgacggtt
     3001 acttctaatt ctggatttga tctccatttt tgccattttt tatgcgattt ttattatctt
     3061 atcagctgga tttttcctgg ataaattttt taatgatcca cgaattccca caaatattat
     3121 cctgccaatt attgccctta ttattgccat aataggggct tttttactcc ataagaacga
     3181 tcataagatc aaattcaatt ttttggttga aggtaaatat cctgaactca aagaaaaact
     3241 caggaccgcc tatgacaata tctctgagtc caatgttatt gtagattctt taaaaagtct
     3301 tgtcctggat ggactggcaa tcgtttcatc ctcaaggcta atacagggaa gtgtaatagt
     3361 cacaaagata atattagtta taatattcat ttccacagca acattcatat cactaaattc
     3421 tgataaatac agcattccgc cggataccat cgccaataat ttcaagaatt taacaggaat
     3481 tgggaatacg gacacgaatg gaacgattga tatcacaggc agccttcaaa atatggaaaa
     3541 tgttgcacaa aacggaagcg ggaacatttt tgggaagcca aagatcgcat ccattgaagg
     3601 gaaaaatatt gaccttaccc tgtattcagg acaggatacc ggttttgaag taagggatac
     3661 aagccaggcc agtaaccagt tcatcaagtc agcggcattc ccggtggatg tccttggttc
     3721 caatgtttct gatggcggtt acagtatgct tatgaaaaag actgaaagtg aaaagcagct
     3781 tatcaataaa tacgcagtgg aaaggagtaa gatatgaaag aggttaaaat atgagtaatg
     3841 agacgtatgc aagctcgacc aggatcttca aagagcttct tgatgagata gggaaagtga
     3901 tcgtgggcca gcacgaggcg gtggaacaga tgatgatagc cattctctgc aacagccatg
     3961 cgcttgttga aagcaacccc gggcttgcaa aaacacttac catcagcaca atttcaaaag
     4021 cgcttgacct taaattcaac aggatacagt gtactcccga ccttatgcct tctgatatta
     4081 caggtacgta tatcatcgag gacatcggcg ggaagaagca gttcaggttt gaacccggac
     4141 ctgtatttgc caatatcgtt cttgcagatg agataaatag agcatcgcca aagacacaaa
     4201 gtgcccttct tgaggcaatg caggaaaaac aggtaacagt tggcaacaaa acttatccgc
     4261 ttgataagcc cttctttatc cttgccaccc agaaccccat cgaaatggaa gatacatatc
     4321 ccctgccgga agcgcagctt gacaggttcc tcctcaagat cttaatggac tatcctacat
     4381 ttgaggatga gaatgagatc gttaacaggt acaccaggaa tattattcca accgtaaaga
     4441 gcgtagtctc aaaaacggaa gtccttgaac tccagaaact cacgcgggat gttcccatag
     4501 ctgaagatat aaagatcagg gcaataaaga tcgtgggcgc cacaaggggg aaaagtgaaa
     4561 atatcgaata cggagcatcg ccaagggctt caattggctt gatcctcgca gcaaaagcgc
     4621 gggcgcttat gaagggcagg aattatgtaa gcaaggaaga tatcgatgcc atggcatatc
     4681 ctgtactgcg ccaccgtgtc atactgacat tcgaatccga gcgcaagggc atgaccaccg
     4741 accaggtgat tgcggatatt ttaaaaaatg cgaaatagat gagctttcga atacctggaa
     4801 tttgtaatta tagcctttgg caaatctatg aaaaaaaaac ctcagattaa caaagatgaa
     4861 cgcagatttg taactattaa tacgcaaagc tcagccatct ctgtgactat gtatgaaaag
     4921 gtgccgccat cacaacgtag aacacggatg acgcggattg cgcggatctt cacggattcg
     4981 tgcgcatcaa ccgttgcgcc agttgacgtc gcccaaccgt cacgtcagtt gacgccgtgc
     5041 acacgtatgc cagtggcata cgtggacgct tcctcaaggg gcgcaaccct tgacgtatgg
     5101 ctcgcgccat acatggagat ccgtgttcca atgcactgca tctgcgttta tctgggttca
     5161 tctgcggtta atactttcaa tgaactcaac tggaaaagtg acctgaatga tagacacctc
     5221 cttcttcaaa gagcttgacc gcttctcatt catggtaaga aagcgcgttt ccaccgcata
     5281 tagcggaagc cggcgctcca tccttaaagg caggggcatg gagcctgtga gctatcgtga
     5341 gtacacacag ggagacgatt ttaagacaat tgactggaaa gtgtacgggc gcactgaaaa
     5401 actttatgtc aaagaattcg aggaagagaa aagcttaacg actcatatat tgctagatac
     5461 gagcaagagc atggatttcc ggaataaatt tgagtattct gcaatgctgg cgcttggatt
     5521 tgcatatctg gtaactaaag acaatgagaa atttgcgatc gccacattcg gtgatgagat
     5581 cattatcacg aaacccaaac gaggaagaag atatctttcc ctgacaatag atcttcttaa
     5641 tagcgcacag cttaatggaa aaacaaactt cgattattgt ttggaaaaat atgcaagtgt
     5701 tattagatca aggtcactgg tctttataat ttctgatttt atggcggatg tcgatgccat
     5761 caggaattca atattcaggc tcggaaataa tgaactcgtg ctaatccagg tgcttgaccc
     5821 cctggaaaaa agccttgacc ttggagggga agcaaaactg atcgatcttg agaccgatgc
     5881 aaaaatggac atctatacaa gccccaggct tcgtgagcaa taccagcgca aactaaatga
     5941 ccatatatct aaaataaagg aaacatgtat gacggtaggg gcggattttc atactgttac
     6001 cacggataag ccggtctttg atgccatttt tgaagttgtg agcagaaggg aagtgaagat
     6061 aaaatagggg gatataatgg cacttcttga ttcattttat gctaacccat tgcagttcgc
     6121 taatgaggct gggctctatg cgcttttgtc aattattccg cttatcataa tttatcttct
     6181 caggccaagg gcaaaaaaaa taaagatccc ttcaatcatg ttccttcttg atattgaaaa
     6241 gaaaaaaagg cttaatattt tcaggaagtt cttgaaagac cccctctttc tcattcaact
     6301 cctggcgctt attatcatct cacttgccat tgcagctcct tttatcatgg cgaacgaaga
     6361 agctggcggc ggttctaccg ttatcatcct cgatggttcc gcgagtatgc aggccgatgg
     6421 caggttcgaa aaagctgttg ttgaagcaaa taagttcttg agcgccaaaa acaccctcat
     6481 ccttgcccag agcctccccc tgatgatact aaaagaagcc ccggcaagca gcgcaaagga
     6541 tgttctttcc aagatcaagg caaaagccac agttgttgac ctgtcaggag caattatact
     6601 tggacgaagg atgcttcc
//
LOCUS       DATGBN010000182         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_402430, whole genome shotgun sequence.
ACCESSION   DATGBN010000182 DATGBN010000000
VERSION     DATGBN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402430"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VKL21_04390"
     CDS             complement(<1..207)
                     /locus_tag="VKL21_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB45041.1"
                     /translation="MKTNMTDHPKVKNYMTTEVDTVSPENSVKDVIELIRRTGHDGFP
                     VVEEGKVTGYVSASGLLEKSLDEKI"
     gene            392..898
                     /locus_tag="VKL21_04395"
     CDS             392..898
                     /locus_tag="VKL21_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMB45042.1"
                     /translation="MNEHDRELLDILKEAISIEIYGREYYSIFGELVEDEDAKSIFRG
                     LSRDEGEHRELLEKQFLMVSGKSFDIGEVDEKNREKAKRIFPESLEPLGIEETKDILK
                     LGIRTEERSIDLYSKSIDKTNNKSSKDLFSRLVHFEGEHKKILEEALYYLDQEGSWYG
                     YSPPTIEG"
     gene            899..1732
                     /locus_tag="VKL21_04400"
     CDS             899..1732
                     /locus_tag="VKL21_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HMB45043.1"
                     /translation="MEGLFFGTAGTPVSTIGTDSLSGIRRVHELGLGCMELEFVRGVK
                     MGEETARKVSVLAKKLDIKLSVHAPYYINLNGEDETRLKSRERILNSARIGQISGAKG
                     VVVHAGFIQKDSRLAAYEKIKQELTGIREQMNNEGLTIALRIETMGRNSQFGSLDEVL
                     DITEVEGVLPCIDFSHLHAVDGKNNSHEEFADILNNIENKLGRDGLDNMHIHVSGIEY
                     SPKGEKKHLEFEESDFNYKELAQVFCEFNIKGNVISESPNLEIDALKLKKECDGSTVR
                     K"
     gene            complement(1715..1855)
                     /locus_tag="VKL21_04405"
     CDS             complement(1715..1855)
                     /locus_tag="VKL21_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45044.1"
                     /translation="MKRCPDCIRPVCQRVLTLCGCKEKPIEEITGVPLNPYMMHDLFT
                     NG"
     gene            complement(1871..2053)
                     /locus_tag="VKL21_04410"
     CDS             complement(1871..2053)
                     /locus_tag="VKL21_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45045.1"
                     /translation="MSESNEVVLWMNKCAPERLASNKVMLCTRNIFLLENMRDLFMDE
                     YVAILKGSGNAGIIKF"
     gene            2230..2403
                     /locus_tag="VKL21_04415"
     CDS             2230..2403
                     /locus_tag="VKL21_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45046.1"
                     /translation="MKDYDSGKSSQKSAAKALGITSRRFRQIHAREQAMENRRSFTIF
                     TRTTGEDLKVWIK"
BASE COUNT          784 a          456 c          538 g          693 t
ORIGIN      
        1 gatcttttca tcaagcgatt tttcaagcaa acccgatgct gatacgtatc ctgtaacctt
       61 tccctcctcg actaccggaa atccgtcatg ccccgtcctc ctgattagtt ctatgacatc
      121 tttgaccgaa ttttcagggg atacggtatc aacttccgtt gtcatgtagt ttttcacttt
      181 tgggtggtct gtcatgttag ttttcaagta aatggttttt cctcttttta taacgtgggg
      241 tagagaagac ctcctgtcat gtggatgatc ctactcctaa tatgaatatt caagtcaatc
      301 taaatattaa tgtgcttacc ttcagacttt catttgtttt ttgtaccaat ctttattacc
      361 aattgaaatc aacctctaca tggtgaatga tatgaacgaa catgacaggg agcttcttga
      421 tatcctgaag gaagcaatat ccatagagat ttatggcagg gaatattact cgatatttgg
      481 cgaactcgtc gaagatgagg atgcaaagtc gatcttcaga ggactttcaa gggatgaagg
      541 agaacacagg gaacttcttg aaaaacaatt cctgatggtc tcaggaaagt cttttgatat
      601 tggcgaggtg gatgaaaaga acagggaaaa agcaaagcgc atattcccgg aatccctcga
      661 acccctgggt atcgaagaaa caaaggatat ccttaaactt ggtatcagga ccgaagaaag
      721 gtcaattgac ctttattcca aaagtataga caaaaccaat aacaaatcaa gtaaagacct
      781 gttctcaagg cttgtgcatt ttgaaggaga gcataagaaa atacttgaag aagcattata
      841 ttatcttgac caggaagggt catggtatgg atattcccct cccactattg aaggttaaat
      901 ggaaggattg ttttttggta ctgcaggaac acccgtaagc acaatcggaa cagatagttt
      961 gtcagggatc cggcgggttc atgaacttgg tcttggctgt atggagcttg agtttgtccg
     1021 cggtgtaaag atgggtgaag agaccgcgcg aaaggtaagt gtacttgcaa agaagcttga
     1081 tataaaatta agcgtgcatg ccccgtatta cataaatctt aatggtgaag atgaaacccg
     1141 acttaaaagc agggaacgaa tactgaattc tgcgcggatc ggacaaataa gcggtgcaaa
     1201 aggagtcgta gttcatgcag gttttataca gaaagattca cgccttgcag cttacgaaaa
     1261 gataaaacag gaactgaccg gcatccggga acaaatgaat aacgaaggtt tgaccatcgc
     1321 gcttcgtatt gagacaatgg ggcgaaattc gcagttcgga agcctggatg aagttctaga
     1381 tattacagag gtggaaggtg ttttgccatg tattgatttc tcacaccttc atgcagtgga
     1441 tggaaaaaat aattcgcacg aggaatttgc agatatactt aataacattg agaataagct
     1501 tgggcgggat ggtctggaca atatgcacat acatgtttca ggaatagaat attcccctaa
     1561 aggtgaaaaa aaacatcttg aatttgaaga gtccgatttc aattataaag agctggctca
     1621 agtgttttgc gaattcaata ttaagggcaa tgtaataagt gaaagcccaa atctggagat
     1681 cgatgctttg aaattgaaaa aagaatgtga tggatcaacc gttcgtaaat agatcgtgca
     1741 tcatataagg attcagggga acccctgtta tctcttcaat cggtttttcc ttgcaaccgc
     1801 ataatgtcag gacgcgctgg cacacagggc gaatgcagtc cggacagcgt ttcatattct
     1861 tcataggaaa ttagaatttt atgattcctg cattgccact gcctttcaat attgcaacat
     1921 attcatccat gaacaggtcg cgcatattct caagcaggaa aatgttcctt gtgcataaca
     1981 taactttatt tgaagcaagc cgctcaggtg cgcatttatt catccacaaa actacttcgt
     2041 tagattcgct cattactctt catctcctta taagttaatc atcataatga ttattatgac
     2101 tatttatatt aatagtataa atagtttgct atgggcaggt tctgttatct tttcccaaaa
     2161 aaatatcctc attctaaaca aatgtagata tgtggtaaaa atcaataaca agcgaagctc
     2221 aaatattatt tgaaggatta tgattcagga aagtcttctc agaaatcggc ggccaaggct
     2281 cttggcatta cgtcaagacg tttcaggcag atccatgcaa gagagcaagc aatggaaaac
     2341 aggagaagtt tcacgatttt tacaagaacc acaggggaag atttgaaagt ctggataaaa
     2401 tgatcgagtg gtacaataat cgccctcacg gatctttgaa tttaagacgg gcagaaacgc
     2461 caaaccaggc a
//
LOCUS       DATGBN010000183         5763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_441403, whole genome shotgun sequence.
ACCESSION   DATGBN010000183 DATGBN010000000
VERSION     DATGBN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441403"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VKL21_04420"
     CDS             <1..323
                     /locus_tag="VKL21_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMB45047.1"
                     /translation="VSSHVNSFGGAAASSLVSIGADVAFVAATRDDIVKVSGRARREI
                     QNAGINLAILMEDISAKFNGTGGGHEGAAGMDASGEIETILRECVAYAKASITKKDIS
                     PLSH"
     gene            complement(306..1835)
                     /locus_tag="VKL21_04425"
     CDS             complement(306..1835)
                     /locus_tag="VKL21_04425"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMB45048.1"
                     /translation="MRGIVFYNIESLRNKKISIFDTTLRDGEQTPGVSLTYDEKLLIA
                     RQLDKLGVDIIEAGFPMSSDGEKASVKKIMDAGLKLQVCGLARVIKSDIDSCLDCGVD
                     MVHTFVSTSDVQRISTIKKSRDEVYSMAIDAVEYIKDHGAKCMFSAMDATRTDIDYLI
                     KIYKGVESAHCDIINVPDTVGVIFPSAMNKLVGEIARNIKIPIDVHCHNDFGLAVANS
                     LAAVEAGASQVQVTINGLGERAGNADLAETVMGLHSLYGAKTNINTQYIVETSHLVER
                     LTEVRMPPTMPIVGENAFAHESGIHTHGVLVRSDTFEPGIMTPEMVGHRRRIVLGKHA
                     GKHAVKQSLEFAGLHPDDEQLNEVMKRIKDIGDKGKRVTDADLYSITESIMGSVSKQE
                     QAIVLKEVSVMTGNIITPTATVKAIVNGKERLGAHIGVGPVDAALNTVTSILGEYKFK
                     LRDFRIEAISGGSDALAEVLIGVEDEKGRIVSARATNEDIVMASVEALVAAINQLMRK
                     R"
     gene            1982..3166
                     /locus_tag="VKL21_04430"
     CDS             1982..3166
                     /locus_tag="VKL21_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMB45049.1"
                     /translation="MKVIRDPIHDYIELDDLALSLIETPQVQRLRRIRQLGFSNLVYP
                     GANHTRFEHSLGVYHLAKHLVKQVDEQQRNELLAAALLHDIGHGPFSHATESVISHYT
                     RKSHEDIEELLRKGEISEVLKKYSLSPSAIARHIRGETNPGQIIHSEIDVDRMDYLVR
                     DAHYTGVAFGLIDHVRLIHELKFNENKLVLNIAGLQAAESLLVSRFLMHPTVYFHHVS
                     RIAESMCHHAAQYMIENGLDPKLLKRMDDRSFMVEMKNAGGYASEIVKRLDERRLFKR
                     ALYTDIHSVNIDVVKLRKSTPRIENEIAQRAGIDPGYVLVDIPETPQIAEMKAQVIIN
                     GKMISIDKASNLVASLEKAQLDNWRLGVFTPAEYRVKVGNIARELFEVKKDIKQFKLT
                     GI"
     gene            complement(3147..3908)
                     /locus_tag="VKL21_04435"
     CDS             complement(3147..3908)
                     /locus_tag="VKL21_04435"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HMB45050.1"
                     /translation="MNAAVFAVENIPLIKKGDDIARILIQRAQLREHDIFVISSTVVS
                     KSEGRIISLEIVNPTERAKLISMKNNVDPRFVQAVLDESMEILLDFPFLLVRIKNGSI
                     CVNAGIDHSNVEGCDNILLLPEDPDKSALDLKRSLFNYTGKNVSVIITDTNGRAFRTG
                     QTGAAIGCAGIKPTRDWRGTKDLFGKVLEVKNEGIADEIAGFANIMMGEGNGGMPMVI
                     IRGLDLYSESNGIKELYRPESEDVICKAILKSRSI"
     gene            complement(4202..>5763)
                     /locus_tag="VKL21_04440"
     CDS             complement(4202..>5763)
                     /locus_tag="VKL21_04440"
                     /inference="COORDINATES: protein motif:HMM:NF036704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HMB45051.1"
                     /translation="LLIYVAMPPIFDTLKNSLTFFSRSGGGLSIAEASPYPFTFENFR
                     NDFGINGVLAFIGLAFLAYGVFKKERQEKTVFLVWTIMMLWALFQQNRFAYYFAVNVA
                     ILSAYFGIAVADKILEFGDWNELAGKKMTNRANIPKRKDIRKLSKTHITQSQDTQGFD
                     FKKIKVSHIISLLIALFLIAYIVYPLIDPAINYGKNGRGMRTYWYESLTWMRYNTPEP
                     GIDFYGKYQEPTVGVNYSYPDTAYGVMSWWDYGHVITAWAHRIPNANPFQSGIGGGST
                     HVPGASTFLSAQSEDEANAVLDAIGANGKPGARYVITDDYINFAAFGAVLSWADITSY
                     DGYYTKVQTNKGSQTVPYTRRYNAMMMRLHLFDGDGLKTYRLVHESVPAPTGDDEAGM
                     GEAGFKDVYNKLFGGNLTVEYTGHVKIFEHVKGAHITGKAPQNATVSISLNVKTNQGR
                     DYAYSQKTVSNGEFELIVPYSTEGPISGETNFDTKPIGKYILIAGNISREVTVNERMV
                     IDGGTEKVDLV"
BASE COUNT         1593 a         1304 c         1196 g         1670 t
ORIGIN      
        1 ttgtttcatc acacgtgaat tcttttggag gggccgcagc gtcaagtctg gtttcaatag
       61 gggcggatgt tgcttttgtt gccgcgacaa gggatgatat tgtaaaagtg agcggccggg
      121 caaggcggga aatacagaat gcagggataa accttgcaat attgatggaa gatattagcg
      181 ccaaattcaa tgggaccggc ggcggccatg aaggcgcggc cggaatggac gcatcagggg
      241 aaattgagac cattctcagg gaatgtgtgg cgtacgctaa agcttcaatt acaaaaaaag
      301 atatttcacc tctttcgcat tagctggtta atggcagcca ccagcgcttc aaccgatgcc
      361 attacaatat cctcattcgt cgcgcgtgct gaaacgatcc tgcctttttc atcctcaaca
      421 ccgattagaa cttctgcaag agcgtcagaa cctcctgaaa tagcttctat cctgaaatcg
      481 cgaagtttaa atttatactc accgaggatg cttgttaccg tatttagcgc tgcatcaaca
      541 ggcccaacgc cgatatgagc cccaagacgt tcttttccgt tcacaatagc ttttaccgtt
      601 gcagtgggcg ttataatatt ccctgtcata actgagactt ctttcagaac gatagcctgc
      661 tcctgctttg atacactgcc cattattgat tctgttatag aatacaggtc agcatccgta
      721 acacgtttgc ccttatcgcc aatatcctta attcgcttca ttacctcatt tagctgctca
      781 tcatccggat gaagtcctgc gaattcaagt gattgtttta ctgcatgttt tcctgcatgc
      841 tttccgagga cgatgcgcct tctgtggccc accatttccg gggtcatgat ccctggctca
      901 aatgtatcgg accgcaccag gacgccatgc gtgtgtatgc ccgattcgtg ggcgaaagca
      961 ttttccccga caataggcat tgtgggcggc atcctcactt cggtcagcct ttccacaagg
     1021 tgcgaagtct caacaatata ctgtgtattg atattggtct ttgcgccata tagtgaatgc
     1081 aaccccatga cagtctccgc aaggtctgca tttcctgccc gctctccaag accattgata
     1141 gtgacctgta cctggcttgc tcctgcttcc acagccgcga gactgtttgc tacggcaagc
     1201 ccgaaatcat tatggcagtg cacatcgata gggattttga tatttctggc tatctcccct
     1261 accaatttat tcatggctga aggaaagatc acgcccacag tatcaggcac gtttattatg
     1321 tcgcaatggg cagactccac acctttataa attttgatca aataatcaat atcggtgcgg
     1381 gtggcatcca tagcagagaa catacatttt gccccatggt cttttatata ttccacagca
     1441 tcaattgcca tactataaac ttcgtccctg cttttcttta tggttgatat cctctggaca
     1501 tcagaagtag aaacaaaagt atgaaccata tctacgccgc agtcaaggca ggaatcgata
     1561 tcggatttta tgacccttgc cagtccgcat acctggagtt tcaatccggc gtccataatt
     1621 ttcttcacag aggctttttc accatccgat gacatgggaa aaccagcctc aataatgtcc
     1681 acaccgagtt tatcaagctg ccgggctatg agaagtttct catcataggt aagcgatacg
     1741 cccggggtct gttccccgtc ccgtagcgtt gtatcaaaaa tacttatttt cttgttcctt
     1801 agcgattcaa tgttgtaaaa gacgatccct cgcattaatc atggttatca agcctcaata
     1861 tcgttttatt gtataaaggc tttttctatt attattatca atctgaaaaa atagttacaa
     1921 aatttggaag ttcaaatgaa taaaattata cggtaaaccg acatatagaa aataaaaact
     1981 tatgaaagtc atacgcgatc cgatacatga ttatatcgaa cttgacgacc ttgcgctttc
     2041 tttgatcgaa accccgcagg tgcagaggct tcgccggata agacagttgg gtttttccaa
     2101 tcttgtttat ccgggcgcaa atcatactcg ttttgaacat tcactgggtg tctatcacct
     2161 tgcaaaacat cttgtcaaac aggtggatga gcagcagcgt aatgaacttc tggccgcagc
     2221 gctgcttcat gacatcggtc atgggccttt ttcccatgcc actgaaagtg tgatttcgca
     2281 ttatacacga aaaagccatg aggatataga agaattatta cggaaaggag aaatttccga
     2341 ggtactcaaa aagtattctt tatccccatc ggcaattgcc cggcatatca ggggagaaac
     2401 aaatccagga cagataatcc acagtgaaat tgatgtggac aggatggatt accttgtaag
     2461 ggatgcccat tataccggtg tggcttttgg gcttattgat catgtcaggt tgatccatga
     2521 actgaaattc aatgaaaata aactcgtact taatatcgca ggattgcagg ctgctgagtc
     2581 cctccttgta tcaaggttcc ttatgcatcc caccgtatat ttccatcatg tcagcaggat
     2641 agcagaatcc atgtgccatc atgctgcaca atacatgata gagaatggac ttgacccgaa
     2701 gttattaaag agaatggatg atcgttcttt tatggtggaa atgaaaaatg caggaggcta
     2761 cgcatccgag atagtaaaaa gacttgatga aaggcggctt ttcaagaggg ccttatatac
     2821 tgatattcat tctgtgaaca ttgatgttgt aaaacttcga aaaagcacgc ccaggatcga
     2881 aaatgaaata gcgcaacgcg cgggcattga tccaggatat gtgcttgttg acattcctga
     2941 aacaccccag atcgccgaaa tgaaagccca ggttattatt aacggtaaaa tgatatccat
     3001 tgataaagcc tcaaatcttg tggcatcgct tgaaaaagcg cagcttgata attggaggct
     3061 tggtgttttc actcctgcag aatacagggt taaggtagga aatatcgcaa gagaattgtt
     3121 tgaagtcaaa aaagatatta aacagttcaa attgaccggg atttgagaat cgccttacat
     3181 attacatctt cgctttcagg tctgtataat tcctttatcc catttgattc actataaagg
     3241 tcaagacccc ttattatgac catcggcatt cctccattgc cttcccccat cattatgttg
     3301 gcaaaacctg caatttcatc tgcaatgcct tcattcttta cctcgaggac ctttccgaac
     3361 aggtcttttg taccacgcca gtcccttgtt ggtttaattc ccgcgcatcc tattgcagct
     3421 ccagtttgtc ctgtacggaa agcccttcca ttggtatctg tgatgatcac actgacattc
     3481 ttgccggtgt aattgaataa tgatctttta aggtcaagtg cactcttatc gggatcttca
     3541 ggtagcagca ggatattgtc gcagccttct acattggagt ggtcaattcc tgcattaacg
     3601 cagatacttc cattctttat acgaacgaga agaaagggga agtcgagcaa tatttccatg
     3661 ctttcgtcaa gcacagcctg gacaaatcgc gggtcaacgt tattcttcat agatataagc
     3721 tttgctcttt ctgtagggtt cacgatttcc agggaaatta tccttccttc agacttggaa
     3781 acaacggtag atgaaataac aaagatatca tgttcacgaa gttgggctct ttgtattaaa
     3841 atgcgggcta tatcatcgcc tttcttgatg agtggaatat tttctaccgc aaatactgct
     3901 gcattcaata aaattcctca ccccccttaa atgcggttgg aataataatt gtttccaatg
     3961 gcagtaataa acatcaacaa aaaatttagt aatagcgcaa agtattaatg tgaaaaagta
     4021 cgactataat ttgtaataca gtagatttgt gggaaaagag ggaattattt caacacgttt
     4081 gtccttatcg tcgttctaaa attatataaa agtaaaaaga tggtcttgag ttattcaaat
     4141 tgtgacatgt gtcaaccgag caccccggaa ctcagggtgc acctcctatt tatagatata
     4201 ttcacacaag gtcaactttt tccgtgccgc catcgatcac cattctctca ttcacagtaa
     4261 cctcgcggga aatattgccg gcaatcagta tatatttccc aattggtttt gtgtcaaagt
     4321 ttgtttcacc ggatattggt ccttcggtgg agtaaggcac gatcaattca aattcgccat
     4381 ttgaaactgt cttctgggaa taggcataat cccttccctg gtttgtcttt acgttcaggg
     4441 aaatggaaac cgtcgcgttc tggggggctt ttccggtgat gtgagcgcct tttacatgtt
     4501 cgaatatctt cacatggcct gtatattcaa cagtaaggtt cccgccaaat aatttattat
     4561 atacatcttt aaatcccgct tcgcccatcc ccgcctcatc atcgcccgtc ggcgcaggga
     4621 cggattcatg caccagcctg tatgtcttca agccatcacc gtcgaacagg tgaaggcgca
     4681 tcatcatcgc attgtacctc ctggtgtatg ggacggtttg agacccttta ttggtctgca
     4741 cctttgtata ataaccatca taagaagtga tatccgccca ggacagaaca gccccgaaag
     4801 ctgcgaaatt tatgtaatcg tctgtgatca cataacgagc gccgggtttc ccgttcgccc
     4861 caattgcatc aagcacagcg tttgcttcgt cttcagattg ggctgataaa aaggtggaag
     4921 ctcccgggac atgtgtcgat cctccgccga ttcctgactg gaaaggattt gcatttggta
     4981 tcctgtgcgc ccatgctgtt atcacatggc cataatccca ccagctcata actccataag
     5041 cagtgtcggg ataagaataa tttaccccaa cggttggttc ctggtacttc ccatagaaat
     5101 caatacccgg ttcgggtgtg ttataccgca tccatgtaag ggattcatac cagtaggttc
     5161 tcatacctct tccgtttttt ccataattta ttgccggatc gataagtgga tacacaatat
     5221 atgctatcag gaataaggct atcaagagtg atataatatg ggaaactttg atctttttga
     5281 aatcaaaacc ttgcgtatcc tgagattgtg ttatgtgcgt ttttgatagt ttcctaatat
     5341 ctttcctttt agggatattg gctctgttag tcatcttttt cccggccaat tcattccaat
     5401 caccgaattc aaggatttta tctgcgactg ctattccaaa ataagcagaa agtatagcca
     5461 catttactgc gaagtaataa gcaaacctgt tctgctggaa taatgcccac agcatcatta
     5521 ttgtccatac aagaaaaacc gttttttcct gtctctcttt cttgaaaact ccataggcta
     5581 aaaatgccaa tccgatgaat gcaagaacac cgttgatccc gaaatcattc ctgaaattct
     5641 caaaagtaaa aggataagga gacgcttctg cgatagatag gcctccgcca ctgcgggaga
     5701 aaaaagtgag ggaattctta agggtatcaa agatcggagg cattgcaaca tagatcaaaa
     5761 gaa
//
LOCUS       DATGBN010000184         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_131018, whole genome shotgun sequence.
ACCESSION   DATGBN010000184 DATGBN010000000
VERSION     DATGBN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131018"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VKL21_04445"
     CDS             <1..306
                     /locus_tag="VKL21_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45052.1"
                     /translation="VGSDEGWSTVFVRTQTGEKYLNKINDIEWTDKPINLDIVKKLTD
                     MKHKHNDWDWRAFMKDIWNRDTPVRPWGAEKLANIPPPPPPSAEKPTGEKPAAKPKE"
     gene            308..820
                     /locus_tag="VKL21_04450"
     CDS             308..820
                     /locus_tag="VKL21_04450"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HMB45053.1"
                     /translation="MRRGIYLGRFQPYHLGHHAVLKQIVNEVDEIIVGIGSAQKSHEI
                     ENPFTAGERVLMVSSALMEFDIKHYVIPIEDIQRNSLWVSHVKSMVPPFDIAYTNNQL
                     VIELLREAGIHVKQSPLFKRNSYSGTEIRRRMLKGEKWEHFVPANVVEIIKEINGVKR
                     IRTVMQSDQE"
     gene            896..2176
                     /locus_tag="VKL21_04455"
     CDS             896..2176
                     /locus_tag="VKL21_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMB45054.1"
                     /translation="MEQKNNSLIECLNDLGYPSNFIQEISEELNKLFKEIDFESIKLR
                     ASQAEQVRDTEGFIKSIKDLMFLLEGKSIYRPDAPIHLIKQLVNGLNLKNQDIFHIIS
                     RSRISMEEKLIEMEFLASCAAITQLGYILLKCIVPDVKAASSGEHVFLVIESFSHDSN
                     IFVDFSIDSVKEIDINIYDRNEKNWKLKKDQDFSTVETETVQYLTEYYSFFHLTRGIG
                     LNHNIHNNLGLAYDKLGRYEEAIDELHAALRLDPDYIEVHNNLAVTYHKMGMTIEAEK
                     ELMEAIRQNPGYLEAHCNLGNIYSTQGRLEEALFELNVALGINPDHAGAHNNLGDIYA
                     IQKRNPEALNEFREALRIDPNYASPHTNIGNLFLESGMYEEAIKEYKEALDIDPLFPE
                     AHYGTGLVHYELNNYEKASQAFIKAVYTSPELMG"
     gene            complement(2166..>2605)
                     /locus_tag="VKL21_04460"
     CDS             complement(2166..>2605)
                     /locus_tag="VKL21_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMB45055.1"
                     /translation="PEEKFDDVRKEFDSAMLNVKMKYPQIELKYSFDEIYAPMLTDMN
                     HPWVKEVQNIASATAGRTVSLAGAQGSLDVAYAVKVTGLPACCFGIGRRTQSNAHADD
                     ENIRIDDLVMFTKFLGKLICGQVKPILKCPALLSELIYQVFTP"
BASE COUNT          831 a          510 c          560 g          704 t
ORIGIN      
        1 gtaggttcgg atgagggttg gagtactgta tttgtgagaa cacagaccgg tgagaaatat
       61 cttaataaaa taaatgatat agaatggact gataaaccta ttaacctgga tattgttaaa
      121 aagctcaccg atatgaaaca taaacataat gattgggact ggcgcgcgtt catgaaagat
      181 atatggaacc gcgacacacc tgtcagacca tggggcgcag aaaaactcgc taatattcct
      241 cccccaccgc cgccatctgc tgaaaaaccg acaggagaaa aacctgcagc caaacccaaa
      301 gaataatatg cgacgtggaa tttatctggg aagattccag ccctatcatc tggggcatca
      361 tgctgtctta aaacagatcg taaatgaagt agatgaaatt atcgtgggca tcggcagcgc
      421 ccagaaaagc catgagatag agaatccttt cacagcaggc gaacgagtgc tcatggtatc
      481 aagtgcactg atggaatttg atatcaagca ttatgtaata ccaatagagg atatccagcg
      541 aaattccctc tgggtttcac atgtgaaatc catggtgccg ccttttgata tagcatatac
      601 caacaatcaa cttgtgatag agctgttgcg ggaagcaggg atccatgtaa agcagtcccc
      661 tctttttaag cgaaacagtt attccgggac agaaataaga cgcaggatgt taaaaggtga
      721 aaaatgggag cattttgtac ctgcgaatgt tgtggagatc ataaaagaga tcaatggtgt
      781 caagaggatc aggacagtga tgcaatcgga ccaggaatga aacatatatt gatctcgtga
      841 actaccccca cctttggagg ggacttttct atccctgatc ccacgagaat aaatcatgga
      901 acaaaagaat aattccttaa tagaatgcct gaatgatctt ggctatcctt cgaatttcat
      961 acaggaaata agtgaagagc ttaataaatt atttaaagag atagattttg aaagtataaa
     1021 actaagagca agccaggcag aacaagttcg agatactgag ggattcatca aatccattaa
     1081 ggatctcatg ttccttcttg aaggaaaaag tatttaccgt cccgatgcgc ccatacactt
     1141 gataaagcag cttgtcaatg ggcttaacct gaagaaccag gatatcttcc atattatctc
     1201 aagatcaaga atatccatgg aagaaaaact aatagaaatg gaatttcttg catcatgtgc
     1261 ggctataact cagcttggat atattctgtt gaaatgtatt gttccggatg tgaaagctgc
     1321 aagttccgga gagcatgttt tcctggtaat tgagagtttt tcgcatgatt caaatatatt
     1381 tgttgatttc agtattgatt cggtcaaaga aatagatata aacatttatg acaggaatga
     1441 aaagaattgg aagttgaaaa aagaccagga tttttcgact gtggaaacag aaaccgtgca
     1501 atatttgaca gagtattact catttttcca cctgacccgg ggaattggtt taaaccataa
     1561 tatccataac aaccttggtc ttgcctatga taagctggga agatatgaag aagcaataga
     1621 cgagcttcat gctgctttgc gtcttgatcc ggattatatt gaagtccaca acaaccttgc
     1681 agtaacttat cataaaatgg gaatgacaat tgaagcggaa aaggaactga tggaagctat
     1741 cagacaaaat cctggatatc ttgaggcgca ctgcaacctt ggtaatatct attcaacaca
     1801 gggcagattg gaagaagcgc tttttgaact taatgtggct ctggggataa atccggacca
     1861 tgcgggcgcc cataataatc tcggagatat ttatgccatt caaaaaagaa atccggaagc
     1921 actaaacgag ttcagggagg cgttgcgtat cgacccaaat tatgcctctc ctcacactaa
     1981 tatcgggaac cttttcctgg agtcgggcat gtatgaagag gcgataaaag aatataagga
     2041 ggctctggac atcgatcctc tttttccgga agctcattat ggaacagggc ttgtgcatta
     2101 tgaattaaat aattacgaga aagcatcgca ggcttttatt aaagcggtat atacttctcc
     2161 tgaactcatg gggtgaatac ctgataaatt aattctgaaa gtaaagcagg gcatttcaag
     2221 attggtttaa cctgcccgca aattaacttt cccaaaaact ttgtgaacat cacaagatca
     2281 tctattctga tattttcatc atccgcgtgt gcattactct gtgttcttct tcctatcccg
     2341 aagcagcagg ctggcaatcc tgtgaccttg acagcatagg ccacatccag gcttccctgc
     2401 gcccctgcaa ggctaaccgt tcttcctgcc gttgcactgg caatattctg gacttccttc
     2461 acccatggat ggttcatatc agtaagcatt ggagcatata tctcatcgaa gctatatttt
     2521 agttctattt ggggatattt cattttcaca tttaacatgg cgctgtcgaa ctctttgcgg
     2581 acatcatcaa atttttcttc aggaa
//
LOCUS       DATGBN010000185         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_208471, whole genome shotgun sequence.
ACCESSION   DATGBN010000185 DATGBN010000000
VERSION     DATGBN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208471"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /locus_tag="VKL21_04465"
     CDS             complement(<1..1931)
                     /locus_tag="VKL21_04465"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HMB45056.1"
                     /translation="MNKKMVILILAMINIAIISGCVQTYKTFDGGNVTIPESTQELKK
                     FSSAYELREYLKASAGNTGAFNVFGGGRDLSGPESKMVATGTAPAMPQPAQAIQAPAA
                     DYSKTNIQVEGVDEADFVKNDGKYIYVLAQDKLVILDAFPASEANILSSTMIEGRPKD
                     IFVNGDRLIVFLEDTDTVYSIQEYDYMPRPRGTMKTVAMVYDISNRKKPEEIANYSLN
                     GNYFQSRMIGDYVYLIAKDYVYYTGDLVDMPSIRKGPVKIMAPDVYYFDNPEQNYVFH
                     TIASINIKKTDSINAKTFMMGYSDNLYVSGNNIYITYRKNLPGRYYEVQRQEQFYTVI
                     LPLLPQDARDKINEINNSNLDMHEKWDKISSILEDTFNRMTEKEKQKYQDDVQKGLEE
                     YEMKLAQEREKTVIQKIGINKGSIEYRTKGEVPGSLLNQFSMDESGDYFRVATTSQFW
                     TSNSNVQYNNVYVMDNDLKIAGRLEKIAQDERIYSTRFIGNRLYMVTFKRMDPLFVID
                     LSNPNKPEILGQLKIPGFSDYLHPYDEKHIIGVGKETAENEWGGVSIKGVKVSLFDVS
                     DVNKPQQIDTYEIGKAGTDSEALQDHKAFLFDKNKNLLVIPVREVTGKEQYDSRYGYN
                     VQKVWQGAYVFNITLDGFKL"
     gene            2089..>3044
                     /locus_tag="VKL21_04470"
     CDS             2089..>3044
                     /locus_tag="VKL21_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45057.1"
                     /translation="MKVSHIFIYIFLILLFSAPVSADFIIENTSTANIPDKDVWSSDI
                     SPDGRTIAYVSYDNSGNQQIFTIKIDGSNRKQLTTDPNKKWGVEWLTNEISYISFDTD
                     EIEKIFIVSLDGSERRKLINETIRQGREPTGSNRFWGAISWDPENKKILFTSLGKNGD
                     EKIFIVNIDGTGTKQLINDNSRQWNPSWSPDGNSFVFISQDNMSTDQLYMASVDGTGM
                     NQVTEDGFKKSDIDWGKEGILFVSTETQIASSKKMYIVNPDGSGQRRLVDQGFNQKNP
                     RWSRDGYTILYEDIDITGSKLIKILSLRKPEATASPVISAVT"
BASE COUNT          864 a          627 c          571 g          982 t
ORIGIN      
        1 agtttgaacc catcaagagt tatgttgaag acataagcgc cctgccatac tttctgcaca
       61 ttataaccgt acctgctgtc gtattgctct tttcccgtga cttcccttac aggaataaca
      121 agcaagttct tatttttatc aaagaggaat gccttatgat cttgaagcgc ctcggaatct
      181 gttcccgctt ttcctatttc gtatgtatcg atctgttgtg gcttattgac atcggacaca
      241 tcaaagagag acactttcac gccttttatt gacacgcctc cccattcatt ctcagccgtt
      301 tccttgccca cgccgattat gtgtttttca tcatatggat gcaggtaatc cgagaatccc
      361 gggatcttga gctggccaag gatttccggc ttatttggat tggaaagatc tatcacgaac
      421 agaggatcca ttctcttgaa tgttaccatg tagagcctgt tgccgatgaa tcgtgttgag
      481 tatatccttt catcctgcgc tatcttttcc agccttccgg ctatcttaag gtcgttatcc
      541 atgacataca cattattgta ctggacatta ctgttacttg tccagaactg tgatgttgta
      601 gccaccctga aataatcccc ggattcatcc attgagaact ggttcagaag acttcccgga
      661 acctcgcctt ttgtcctata ttcgatactg cccttattga tgcctatttt ctggatgaca
      721 gtcttttccc tttcctgcgc aagtttcatt tcatattctt caagcccttt ttggacatca
      781 tcctgatatt tttgtttttc tttttcagtc atccggttga aagtatcttc aagaatagat
      841 gatatcttgt cccacttctc atgcatgtca aggttactgt tattgatctc attaatttta
      901 tccctggcgt cctgtggaag cagtggcaga atcacagtat agaattgctc ctgcctctgt
      961 acttcataat atcttcctgg caggttcttc ctgtacgtta tatatatatt attacccgat
     1021 acgtagaggt tgtcggaata acccatcatg aaagtttttg cgttgatgct atctgttttc
     1081 ttgatattga tagacgcgat cgtatgaaat acgtaatttt gttcgggatt atcaaaataa
     1141 tacacgtcag gagccatgat cttcacaggc cctttcctga tcgaaggcat atccacaaga
     1201 tcaccggtat aatatacata atcttttgcg atcaaataaa cataatctcc gatcatcctt
     1261 gactggaaat aattgccgtt caaactgtaa ttcgctatct cttcaggttt ctttctgttt
     1321 gagatatcat ataccatggc gactgttttc atagtgcccc ttggtcttgg catataatca
     1381 tattcctgga tactatacac cgtgtcagta tcttctaaaa agactatgag cctgtcaccg
     1441 ttcacgaata tgtcctttgg tcgtccttct atcattgtgg aagaaaggat atttgcttcg
     1501 gatgctggaa atgcatcaag gatcactaat ttgtcctgcg caaggacata tatgtatttt
     1561 ccatcgttct ttacaaaatc agcttcatcc acgccttcaa cctgaatatt cgtttttgaa
     1621 taatccgctg caggcgcctg gatagcctgc gctggttggg gcattgccgg cgctgtccct
     1681 gtggccacca tttttgattc tggcccggaa agatctctac ccccaccaaa aacattaaat
     1741 gcgcctgtat ttcctgcgct tgctttaaga tattctcgca attcataagc tgaagagaat
     1801 ttctttaatt cctgtgttga ttcggggatt gtaacattcc ccccgtcaaa tgttttgtag
     1861 gtctgtacgc acccgctaat aatcgcaatg tttatcatgg ctaatatcaa aattaccatt
     1921 ttcttgttca attagtatca cctttatgat actatcaggc ggtatgaaga taaatatatc
     1981 tttaacttcg aattgaattg gagttatggt ttaatcccta atccaaagtc tctaaaataa
     2041 caacgcaaca tttatcatgt taaatagata tataacgaga taggttttat gaaagtatca
     2101 catatattca tatatatttt tttaatattg ttattttcag cgccggtttc agctgacttt
     2161 attattgaaa acacttctac tgccaatatt cctgataaag atgtgtggag ttccgatata
     2221 agtccggatg gcagaactat cgcatatgta tcttatgata attcagggaa tcagcagatt
     2281 ttcacgatca agatagatgg ttcaaataga aaacagttaa ccactgaccc aaataagaaa
     2341 tggggagtag aatggttaac aaacgaaatc tcttatatat cttttgacac ggatgaaata
     2401 gaaaaaatat tcattgtttc attggatgga tcagaaagaa gaaaactgat caatgaaact
     2461 atccgccagg gaagagaacc tacagggagt aaccgtttct ggggtgcaat ttcgtgggat
     2521 cccgaaaata aaaagatatt atttacatca ctgggcaaaa atggtgatga aaaaattttt
     2581 atagtgaaca tagatggaac tggaacaaaa caattaatca atgacaattc tcgccagtgg
     2641 aatccaagtt ggagccctga tggtaattcc tttgttttca tctcccagga caatatgtcc
     2701 accgatcaat tgtatatggc cagcgttgat ggcacgggta tgaaccaggt cacagaagat
     2761 ggattcaaaa aatctgatat tgattggggc aaagaaggga ttctttttgt atcgactgaa
     2821 acccagatag caagcagtaa aaaaatgtat atcgtaaatc ctgatggctc cggacaaaga
     2881 cgactcgtag atcaaggttt taaccagaaa aatccgagat ggagcaggga tggttatacc
     2941 attctttatg aagatataga cattacaggt agtaaattaa ttaagatatt aagtcttaga
     3001 aaacctgaag ctacggcatc acctgtaatt tccgccgtta caat
//
LOCUS       DATGBN010000186         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_519297, whole genome shotgun sequence.
ACCESSION   DATGBN010000186 DATGBN010000000
VERSION     DATGBN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519297"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VKL21_04475"
     CDS             complement(<1..1165)
                     /locus_tag="VKL21_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45058.1"
                     /translation="MNNFRCVFICILAFAMLTGLAASSEVVIENPIPVGVPDQVPAPN
                     FTKLEITPQYGNFMLQPGNSKEMTVTIKNKENKAVSVKPTTVLMPYSQYNVDPEWISV
                     KPGSAEIPAGGSQKFTIKVNVPSDATTGNSGVQIAFTNETMPMPYPMPVPSYVHVFQL
                     SIDIWTTPKIQIAPQYIYDSLQAGEEYDYEIKLKNIGKESINIDPKLSNDNPYGQMPA
                     AFPDDAITITAPSKVPAGATEVVKVHVNVPADSKGAYNGGINLNIDDPSIRDYEGRVQ
                     LNFNVWRQPTEAFVKSFSMKEAAPLTIEISSGFSNPYPYGMGTNKKQPSFETKLVGPD
                     GNAALKVTKTVIKGGVSMGGEMPPWELDSKGIYQDSGIQFIETYKANGSKGEWK"
     gene            complement(1305..>2173)
                     /gene="sppA"
                     /locus_tag="VKL21_04480"
     CDS             complement(1305..>2173)
                     /gene="sppA"
                     /locus_tag="VKL21_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMB45059.1"
                     /translation="TVLFLLFLITGAFYLILGGKDLGISNNRVEVIYVQGIMLTGSVP
                     TGFGIATSEEITKSLKDADEDEDIKAIVMRINSPGGSPASAEEIVSTMKKIDKPIVIS
                     MGDVAASAAYYISAPADKILANPDTMTGSIGVIWEFQNRSQFYKKEGTSFYIAKSGEL
                     KDMGGDWKGLSDEEKQYADQVISEAYGRFVKEVADDRNLSLSQVKDIADGRIYTGTKA
                     KELGLVDDLGSLDDAIEIAAKLGGIVGKPEVGYANKPSLSRLLFGSEKTDISVFTSYF
                     FESPFGWLLSIS"
BASE COUNT          544 a          517 c          419 g          693 t
ORIGIN      
        1 gcttccattc acctttcgaa ccatttgcct tataggtttc aataaactgg ataccgctgt
       61 cctggtatat gcccttgctg tctagttccc atggcggcat ttcaccaccc atgcttacgc
      121 ctccttttat cactgtcttt gtgactttga gggcagcatt tccatcagga ccaacaagct
      181 tcgtttcgaa tgagggctgt ttcttatttg tccccatacc atacggatat ggattagaga
      241 aaccggagct tatctcaata gtcaagggtg cagcctcttt catactgaaa cttttcacga
      301 aggcttcagt aggttgtctc cagacattga agtttagttg tactcttccc tcataatccc
      361 tgatagaagg gtcatcgata ttcaggttta ttcctccgtt gtaggcacct ttcgaatccg
      421 caggcacgtt gacatgcact tttacaactt ctgtggctcc tgcaggaact ttcgaaggag
      481 ctgtaattgt tattgcatca tccgggaaag ctgcaggcat ctgcccatat ggattatcgt
      541 tgctcaattt cgggtcgata tttattgatt ctttcccgat gttcttgagt tttatctcat
      601 aatcatattc ttcaccggct tgaagactat cataaatata ttgtggcgct atctgtattt
      661 ttggcgtagt ccagatatca atggaaagct ggaaaacatg gacataactg ggaacaggca
      721 ttggatatgg cattggcatc gtttcattag tgaatgctat ctgtacgccg gaattgcctg
      781 ttgtggcgtc agagggcacg ttaactttaa tagtgaattt ctggcttcct cctgcaggta
      841 tttccgcact tcccggcttg acagatatcc actccggatc cacattatat tgactatatg
      901 gcatcaatac cgtagtgggt ttgacactca cggctttatt ctctttgttc ttgattgtga
      961 ccgtcatttc tttactattt cccggctgca acatgaaatt gccatactgg ggagtaattt
     1021 ccagttttgt aaagttgggt gcaggcacct ggtcgggcac acccacagga attggatttt
     1081 caatcacaac ctcgctgctt gccgccaaac ccgtaagcat ggcaaatgca agtatacata
     1141 taaaaacaca tctaaagtta ttcattgttt aacctcctga ctctatatat tttcaagttc
     1201 gtctaaaaaa agacaaaccc actcttaatt gcaaatacat aataataaat cttgcggcag
     1261 atatttcaat cttttaatat aaagacctct tccattgaga tgcattatga tatcgataaa
     1321 agccagccga acggactctc gaaaaaatag cttgtaaata ccgaaatatc tgttttctca
     1381 ctgccgaata ataaacggga gagggatggt ttgttggcgt atcctacttc gggttttcca
     1441 acaatacccc cgagttttgc agcaatttca atagcatcat caaggcttcc cagatcatcc
     1501 acaagaccaa gttcttttgc ttttgttccc gtgtatatcc tgccgtccgc aatatccttt
     1561 acctggctca atgagaggtt tctgtcatct gccacttctt taacgaaacg gccgtaagct
     1621 tccgagatta cctggtctgc atactgtttt tcctcatcgg ataacccttt ccagtccccg
     1681 cccatgtctt ttaattcgcc tgattttgct atataaaaag atgtaccttc cttcttgtaa
     1741 aattgcgacc tgttctggaa ttcccatatc acacctatac ttccggtcat tgtatcgggg
     1801 ttggcaagta tcttatcggc gggagcgctt atataatatg ccgcgctagc agcaacatcg
     1861 cccattgaga tgactatcgg cttatcaatt tttttcatcg tggatacaat ttcttcagca
     1921 gatgcaggag aacctcccgg gctgtttata cgcataacga tagctttaat atcttcatct
     1981 tcatctgcgt ctttcaagct ttttgttatt tcctccgatg ttgctatccc gaacccggtg
     2041 gggacactgc ccgtcagcat tattccctgg acatatatta cttcgaccct gttatttgaa
     2101 atgcccaggt ctttgccccc caggatcaaa taaaaggcgc ctgttatcag gaacaacaag
     2161 aaaagtacag tga
//
LOCUS       DATGBN010000187         4424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_402823, whole genome shotgun sequence.
ACCESSION   DATGBN010000187 DATGBN010000000
VERSION     DATGBN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402823"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VKL21_04485"
     CDS             complement(<1..455)
                     /locus_tag="VKL21_04485"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HMB45060.1"
                     /translation="MRYQIMEKDKIWTYISDILVILSDAVGKNMTGSARYLSILAIVG
                     FTALVFSASPALAATAPTLGTAQSYGVLGHTTVTNTGPTVVNGDLGLSPGTAVTGFPP
                     GIATGTIHTADAAALQAQNDVTTAYNNLAGQTCDFDPFGPTDLAGQTLVP"
     gene            833..1546
                     /locus_tag="VKL21_04490"
     CDS             833..1546
                     /locus_tag="VKL21_04490"
                     /inference="COORDINATES: protein motif:HMM:NF003140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMB45061.1"
                     /translation="MAKKEETKEVKETKKEAPKKEAPKQEALPEPKKEKKQDKKHEGK
                     GEGKEHEKKDRKGAPKEGKKDAPKAKETGPEIKHFVRIANTDLEGKKSVQYGLIGIKG
                     ISRRTAKILTVNSNVDPKATLGYLADADIERLQLSVDSISSILPVWMVNKQNEIMSGE
                     DRHLIGTDVLLGLNEDLNTMKKMRSYKGLRHDRGLRVRGQRTRSSGRRGRTVGVSRTA
                     LMAKPKEAEKKEETKGAGK"
     gene            1546..2148
                     /locus_tag="VKL21_04495"
     CDS             1546..2148
                     /locus_tag="VKL21_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMB45062.1"
                     /translation="MGYPGKNKKSYNTPKHPWQAARIEPEVELVKRYGLRNKREVWKA
                     HSELKKYRELARKLLAESIKGKLEGHIKTDGENILNRLKRYGLLKTDAVLDDILSLDV
                     NNFLERRLQTQVHKQGLANTHKQARQFIVHGHIAVAGRKITVPGYLVPANEELSLGYY
                     TGSELSKESHPSRPGQVVKNIVQDTTPSNETRNVPGREVK"
     gene            2148..2528
                     /locus_tag="VKL21_04500"
     CDS             2148..2528
                     /locus_tag="VKL21_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMB45063.1"
                     /translation="MATEKWAVAHIYSSFNNTLITVTDLTGAETIAKISGGMVTKADR
                     EESSPYTAMQMANQLADKIRDKGIIGIHIKVRAPGGNMQRSPGGGAQAAIRAFARAGI
                     KIGRIEDVTPIPHDGTKPKKGRRV"
     gene            2529..3314
                     /locus_tag="VKL21_04505"
     CDS             2529..3314
                     /locus_tag="VKL21_04505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HMB45064.1"
                     /translation="MDIDIIELSERKARFVISDVSASFANALRRSILAEVPVMAIDDV
                     NIYENTSVLFDEQLALRMGLIPIKTDNKSYVLPEECNCKGAGCTLCKVSLTLSAEGPK
                     VVYSGDLVSTDPMVEPADKTVPIVELKEKHKVVVEAIARLGTGRKHSKWQAGVAAGFK
                     NMPVVTIGECDYCGNCVEVCPRNILKMEGNKVKVVNVIECSMCRLCEDKCEMDSIKVT
                     GKPESFVMTFETTGAMTAKEIALEAAGSIKNRAEQLSEIVDAL"
     gene            3384..3468
                     /locus_tag="VKL21_04510"
     tRNA            3384..3468
                     /locus_tag="VKL21_04510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3420..3422,aa:Leu,seq:gag)
     gene            3614..3847
                     /locus_tag="VKL21_04515"
     CDS             3614..3847
                     /locus_tag="VKL21_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45065.1"
                     /translation="MKRKELSEASVRRNYAFSQRTIENFNWLIDHKIYRNETEAIDLA
                     IERMRTIYEMKEEESKASNPEKPDDIKTSIKVN"
     gene            complement(4039..4386)
                     /locus_tag="VKL21_04520"
     CDS             complement(4039..4386)
                     /locus_tag="VKL21_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMB45066.1"
                     /translation="MVGHTKFLKKRLYLFPLIGSIADKPYNTNQLAEALNIDYQITSH
                     HLDILANNGIISTECDNYCRTYFLSKNMEGNFKNFIQHHGWAVPARGFERLAKTIRQA
                     PGIRINSWEAEHL"
BASE COUNT         1435 a          849 c         1028 g         1112 t
ORIGIN      
        1 ggaacgagcg tttgtccagc cagatccgtc ggaccaaagg ggtcgaaatc gcaagtctgg
       61 cctgctagat tgttgtacgc ggtggtgaca tcattctgag cctgaagcgc ggccgcatca
      121 gctgtgtgta tagttccggt ggcgatcccg ggaggaaagc cagtgactgc ggtacccggg
      181 ctgagaccaa gatccccgtt gactactgtc gggcctgtat tggtcacagt cgtatggccc
      241 agtaccccat agctttgcgc cgttcccagt gttggcgcgg tcgcggccaa tgccggggag
      301 gcgctgaaaa caagcgcggt gaatcccacg attgccagaa tactcaagta cctggcactt
      361 cctgtcatat ttttaccaac agcatcagat aaaattacta atatatctga tatatatgtc
      421 catatcttat ccttttccat aatttgatat ctcatatttc cctcccaaag attttatagc
      481 gggggagatc atatttatta acgtattccc gcacacactt tgagcatcat tttatgtgag
      541 tttcagggat ataaaaacat cattgagaaa tatgctcatc tatatggatg cctgttcgta
      601 caggttaatg tcaaaataac atatcacagg cggttaccca ctttttttct ggaccaccaa
      661 ctcatcaaca ttataatgat actatattag attttctgtt tgatttatga gaatttatgg
      721 aaaaattctc tcgtttgata cataaaatat aaatcctatg caaatgtatg agggctgccc
      781 aatgtgaggg ctgcgccgga taattccggg gctaaagcca caggaggaaa acatggctaa
      841 aaaagaagaa acaaaagaag taaaagaaac caagaaagaa gctcctaaaa aagaggctcc
      901 caagcaggaa gctctccctg agccaaagaa agagaagaag caagacaaga aacatgaggg
      961 gaaaggggaa ggaaaagaac acgaaaagaa agatcgtaaa ggcgctccca aagaaggaaa
     1021 gaaagacgct cctaaagcca aggagaccgg gccggaaatc aaacattttg tgcgtatcgc
     1081 aaatactgac cttgagggca agaaaagcgt ccagtatggc ctgataggaa taaaaggcat
     1141 aagcaggcga acggcaaaaa tattgacagt gaattcaaat gttgacccta aagcaacatt
     1201 gggttatctt gccgatgcgg atattgagag attacaatta tcagtagata gcatatcatc
     1261 tattctcccg gtgtggatgg ttaataaaca aaacgagatc atgtcgggtg aagacaggca
     1321 tcttatcggt acggatgtat tactgggtct taatgaagac ttgaacacta tgaaaaagat
     1381 gcgttcatat aaaggattaa gacacgaccg cggtctaaga gtacgcggcc agcgcaccag
     1441 atcctcaggc agaagaggaa ggaccgttgg tgtctcacgc accgccctta tggcaaaacc
     1501 caaagaagcg gaaaagaagg aagaaacaaa aggagctggt aaataatggg ctacccggga
     1561 aaaaacaaga aatcatataa cacgcccaag cacccatggc aggccgcaag gatcgaacct
     1621 gaggtcgaac ttgtgaaaag atatggtctc aggaacaaga gagaagtctg gaaagctcac
     1681 agcgaactta agaaatacag ggaacttgcc agaaaactgc ttgctgaaag tataaaaggt
     1741 aaacttgaag gacatattaa aacagatgga gaaaatattc ttaaccgtct gaaaaggtat
     1801 gggttattga agactgatgc cgtgcttgat gacatattgt ctcttgatgt aaataatttc
     1861 cttgagagaa gacttcagac acaagtgcat aagcagggac ttgcgaatac ccataaacag
     1921 gcccggcaat ttattgtcca tggacacatt gccgttgcag gacgaaagat caccgtccct
     1981 ggttatcttg ttccagccaa tgaggaatta tcattaggtt attatacagg ttccgaatta
     2041 tcgaaagaat cgcatccttc aagaccggga caggtagtca agaacattgt gcaggataca
     2101 actccatcaa atgaaacaag aaacgtaccc gggagggagg ttaaataatg gcaactgaga
     2161 aatgggccgt agctcatata tattcctcgt tcaacaatac cctcataaca gtaactgatt
     2221 taacaggggc tgaaacgata gccaaaataa gcggcggtat ggttacaaag gctgacaggg
     2281 aagagagttc cccttatact gccatgcaga tggctaacca gcttgctgat aagatacggg
     2341 ataaaggaat aataggcata catatcaaag taagagcccc cggcggcaac atgcagagaa
     2401 gtcctggcgg aggggcgcag gctgccatca gggcgttcgc gcgtgccgga ataaagatcg
     2461 gcaggatcga ggacgtgacc ccgatacctc atgatggaac aaaacctaag aagggcaggc
     2521 gtgtgtaaat ggatattgac ataatcgaat tatccgagcg caaggcgaga ttcgttatat
     2581 ccgatgtttc tgcatcgttt gcaaatgctt taagaagaag cattcttgca gaagttccgg
     2641 taatggctat tgatgatgtt aatatttatg agaatacttc cgtattgttt gatgaacagc
     2701 ttgcattaag gatgggtttg atcccaataa agacagacaa taaatcctat gtgcttcctg
     2761 aagaatgtaa ctgcaagggt gcaggatgta ctttgtgtaa ggtatcattg acccttagcg
     2821 cggaaggtcc gaaagttgta tattccggag atctggtttc aaccgatccc atggtggaac
     2881 cggcagataa aacggtacca attgtggaac ttaaggaaaa gcataaagtg gtagtagaag
     2941 ccatagcaag gctcggaaca ggcagaaaac acagtaaatg gcaggctggt gtcgcagcag
     3001 gatttaagaa tatgccagtt gtgaccatcg gagaatgcga ttattgtgga aactgcgtgg
     3061 aagtatgtcc aaggaacatc ctgaaaatgg aagggaacaa ggtcaaggtc gttaacgtaa
     3121 ttgaatgttc catgtgccgg ttatgcgaag ataaatgcga aatggattca attaaagtga
     3181 caggtaaacc ggaatccttt gttatgacct tcgaaacaac aggcgctatg accgctaagg
     3241 aaattgctct tgaagcggca ggtagtatta agaatagagc ggaacaattg agtgaaatag
     3301 ttgatgccct ctaataagaa gagaatatga tctcttttat aatataaata tttcataatg
     3361 ctttgaaatc aaagcattca tttgcggagg ttgcccagac aggtcaaagg cgccagcttg
     3421 aggggctggt ttcgaaggaa ttcgtgggtt caaatcccat cctccgcata aatattcaat
     3481 gaattttttt aactctctca atagtaacat atttttttta tcttagtgtt ttattttttt
     3541 tctgctttac ctatccattt gatcatttca gtccaaaggt ataaatatgt atacgtacta
     3601 taatacgtac ataatgaaac gaaaagaatt gagtgaagca agtgtgcgaa gaaactatgc
     3661 attcagtcag cgcacaattg aaaattttaa ttggctcata gaccacaaga tatacaggaa
     3721 tgaaactgaa gcaattgatc ttgcaattga acgcatgcgg actatctatg aaatgaagga
     3781 agaagaatca aaagcgtcaa atccagaaaa acccgatgac atcaaaacgt caataaaagt
     3841 taactgaaag gattaggcct aattaggaaa tatacgataa aattaaagca atccaggatc
     3901 gctatgaaga tttgatttca ttctcagacc tttccacggt tcgtttcgca gtctctgtgg
     3961 tgaacatctc aacagctctt gtcaactatc tgaacactct caggcctgat gctggtatac
     4021 gagcctccat ttttcggttt atagatgttc ggcttcccaa ctattgatac gaatgccggg
     4081 tgcttgtctg atagtcttgg caagacgttc gaaccctctg gctggtactg cccagccatg
     4141 atgttgaatg aaatttttaa aatttccctc catatttttg gaaagaaaat atgttctaca
     4201 ataattatcg cattctgttg agattattcc gttattggca aggatatcga gatggtgact
     4261 ggtaatttga taatcaatat tcaaagcttc agctaattga tttgtattat acggtttgtc
     4321 ggcaatcgac cctatcaggg gaaagagata caatcttttt ttcaggaact ttgtatgccc
     4381 aaccactgtg gctatttata tacaaagtgc tttcgttaaa gtct
//
LOCUS       DATGBN010000188         5094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_208613, whole genome shotgun sequence.
ACCESSION   DATGBN010000188 DATGBN010000000
VERSION     DATGBN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208613"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /locus_tag="VKL21_04525"
     CDS             <1..1949
                     /locus_tag="VKL21_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB45067.1"
                     /translation="VVYFGDVEPSSSSDVKELTKDLNQVITLSPTGKVDAVFFMGDMT
                     KDSGTFQLTLNALSASTLKNTPAYFVIGNHEYNSKSTTYPLIKNKYSSSVIPLNYFPA
                     DTQKTTFSMNVGNIHVIDMNEYWDNSGGAVSNPLYNWMSTDLSGTTNYKIVIGHDPLY
                     PLKKHVGDSLDANTANRDRLQSLFVSKNVGVFLGGHTHYSSVQNKNGVLHVATGVIGS
                     GTSQGEDKFATITYTYTTPENLILTIKQENPTWSTPKIITYTIGGTTPTPTITATPTV
                     TATPKVTVTPTVTATPKVTVTPTVTATPPAPSGSCYITPGGVPSYMTQYVGKTVCANC
                     GHHNPISSAWKPCTTPTPTVTATPTVTVTPTVTATPTPASGSTIAIFGDAHTTESNDR
                     VPQLTSDMNNYASLSPTGKIDAMFTTGDMEPVTKVDQAHQTSTLKNIPLYYTIGNHDT
                     GDVSKIKALQPTNLKINPGPTGNEKSSFSVEVGNIHVVILDAYWDGKTNEGWMGGGSS
                     GGEIGTTLTNWLKDDLQSATTKYKIVMDHEPMYPIKRHVGNSLDYDTKSRDALQAMLD
                     SQGVDAFFAGHTHYATGELHGGVLQVNDGVPGTKAGTDADSFRSVWFVNIATNGDLVI
                     TWKHNANSGSSWSNPIVKTWTLKQ"
     gene            complement(2238..2594)
                     /locus_tag="VKL21_04530"
     CDS             complement(2238..2594)
                     /locus_tag="VKL21_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45068.1"
                     /translation="MTKGMAGFEVDIFKKCVEVDLHKYSISTALIAAREKIKEAYGHG
                     FRYIRLIHGAANITSKNERKSIKFSLRIKLKSGEFDKWVDKKGSVFRDESLILEIRKN
                     SAPLNREWNEMPLEEY"
     gene            complement(2587..4491)
                     /locus_tag="VKL21_04535"
     CDS             complement(2587..4491)
                     /locus_tag="VKL21_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45069.1"
                     /translation="MSIIPVIKKDFLVYVRHRKTLILIFLAPILIMILIGSVFSGASD
                     QGLKEVKLGVGGGSEMGDDIIEELNNSKMFIIVKENTNDPSVIEDGVMKGKYSAGIFI
                     PENQTQSMKLFIDNSRVQIAPVITAVFLTTTEKMSYELTLGFISKLWENLGEMESELG
                     PLKKGVFSINEDIGKLNNDTQNVLVRLDEINVTRMNESINMMKNTLDQMKIDLSQTSV
                     DINTTRNDILELDKNVSSIYNDSATLRDDLKLVVDNIDSTDAALLAIRTDLQGTYNST
                     CAIPSTPQCISIANTIRQIQDTRVLLIEKTDRIRSLYNGLANVAQKGSELHEKLERTD
                     IKLQNMQELMGNYTFEISNIRGNISNIETATVILDQVKVRSANVSVQMNNLSTEMTNS
                     TQDLVSEIDRTKSILGEVRIKSPAAIAAPIKLERTPVFREMSYLDFLMPAIVSIVLMF
                     ISFLLSSITIVQERTKKTLLRTLLTPLSLGEFIFAKTSALILIAMLQGIILVVVAFLF
                     YGVLISASQLGLLFLVILVYSAAFIGIGMAVATFAESENTAMLSSLVLSIPMLFLCGV
                     FFPFETMPQLMVKVGSALPITMGIRALDSVLIYREGFGMLARYLLPLLLYGITGIGLA
                     YILLRKEISD"
     gene            complement(4488..>5094)
                     /locus_tag="VKL21_04540"
     CDS             complement(4488..>5094)
                     /locus_tag="VKL21_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB45070.1"
                     /translation="IEIKKRSHKILTLLKLDEKSNIRANNLSGGMKTRLNIACALIHK
                     PEVLILDEPSVGLDPVSRKALWETIQVVNDEGTTILLTTHYMEEADLLCDRILIMNRG
                     KIVIEGKQDELKKKVGEKMILITSSPGNYSVLKDNVLSLGNISSCSVSDNGLKITHTG
                     KVQLHKIFDVFESLEEKIITVESSTTSLEDVFIHASGEEWH"
BASE COUNT         1630 a         1039 c          926 g         1499 t
ORIGIN      
        1 cagtagttta ttttggtgac gtagaaccat caagttcatc tgatgtaaaa gaattaacta
       61 aagacctgaa tcaagtaatt actttatcac caactggtaa agtcgatgca gtattcttta
      121 tgggtgatat gacaaaagac agtggtactt tccaattaac attaaatgct ttatcagcat
      181 ctactttgaa aaatacaccg gcttatttcg tgataggaaa tcacgaatat aattcaaaaa
      241 gtactacata tcctttaata aagaataaat attcatcttc agtaattcca ttaaattatt
      301 tccctgctga tacgcaaaaa actacgtttt caatgaatgt tggaaatata catgttatag
      361 atatgaacga atattgggat aatagtggcg gagccgtttc aaacccgttg tataattgga
      421 tgagtactga tttgtccggt acaactaatt ataaaatagt tattggccat gacccattat
      481 atcctttaaa gaagcatgta ggcgattcat tagatgcaaa tacagcaaat cgtgatagac
      541 tccagtcgtt atttgtttcg aagaatgttg gagtattcct gggtggccat acacattatt
      601 cgagtgttca aaataagaat ggagtattac atgttgctac tggagtaatt ggttcaggaa
      661 caagtcaggg tgaagataag tttgcaacaa ttacatacac ttatactaca ccggaaaatt
      721 taatcttgac aataaaacaa gaaaatccaa cctggagtac accaaaaatc ataacatata
      781 caattggtgg aacaacacca acacccacca taacggcaac tcctactgta acagcaacac
      841 ccaaagtaac agtaacacct actgtgacag caacacccaa agtgacagta acacccaccg
      901 taacggcaac accccctgct ccctccggat cgtgctatat caccccaggt ggcgttccat
      961 catatatgac acaatatgtg ggaaaaacag tttgtgcaaa ttgcggccac cacaacccaa
     1021 tatcttcagc atggaaacca tgcacaacac caacgcctac tgtgacagca acacctacag
     1081 taacagtaac acctactgta acagcaacgc caactccagc atcaggttca acaatagcaa
     1141 tttttggcga tgctcataca acggagagta atgacagagt tccacaacta acatcagata
     1201 tgaataatta cgcatcttta agtcctactg gaaaaataga tgccatgttt acaactggtg
     1261 atatggaacc agttacaaaa gtagaccaag cacatcaaac atctacatta aaaaatattc
     1321 cgctatatta tacaataggt aatcatgata caggagatgt ttcaaaaata aaagcattac
     1381 aacccacaaa tcttaaaatc aatcctggcc cgactggaaa tgaaaaatca tcgttttctg
     1441 tagaagtagg aaatatacat gtagtaatac ttgatgcata ttgggatggt aaaacaaacg
     1501 aaggatggat gggaggcgga agcagtggcg gagaaatagg aacgacatta actaattggt
     1561 taaaagatga ccttcaatct gcaacaacaa aatataaaat tgtaatggat cacgaaccaa
     1621 tgtatccaat aaaaagacat gtaggaaatt ctttagatta cgatacaaaa tcgagggatg
     1681 cattacaagc tatgcttgat agtcaaggcg ttgatgcatt ttttgctgga catacacatt
     1741 atgctactgg tgaattacat ggaggagtac ttcaagtaaa cgatggagta ccgggaacaa
     1801 aagcaggaac agatgcggat tcatttcgtt ctgtttggtt tgtaaatatt gcgacaaacg
     1861 gtgatttagt aataacatgg aaacataatg caaatagtgg aagttcatgg agtaatccga
     1921 tagttaaaac atggacactt aaacaataat aaggagaaac aatatgataa aaaaaaatat
     1981 taattctctt tatttttttt atatatatta attcaacaat ggcggctaca aatataatat
     2041 caaatccttg gtttgaatct ggaacaacag taccaactga tttgaagttt gaaacaaaaa
     2101 atggaaatag accaaaaatc gcaacatata caattagtaa ataaagaaac agaactgcaa
     2161 ttaaacaaat acccccgctt agaaagcggg ggcacctctc gtttgatcag atttcctttt
     2221 ttcaaaaaca attcgagtta atattcctcc aaaggcatct cattccattc ccgatttaat
     2281 ggagctgaat tcttcctgat ttcaagaatt aaggattcat cccgaaaaac cgagcctttc
     2341 ttgtctaccc atttatcaaa ttccccactt tttaacttta tgcgcaatga gaactttata
     2401 ctcttgcgct cattcttact cgtaatattt gccgctccat gaattaatct tatatacctg
     2461 aacccatgcc cataagcctc tttgatcttt tctcgggctg caattagtgc agtgcttatt
     2521 gagtatttat gcaggtcaac ctctacacat ttcttgaaga tatctacttc gaacccggcc
     2581 atacctttag tcacttattt cctttcttaa caaaatatat gccagcccga ttcccgtaat
     2641 gccatataga agaagcggca gaagatatct tgcgagcata ccaaaccctt cccggtagat
     2701 caatacagaa tcaagagccc ttatgcccat agtgatcgga agggcgctcc cgacttttac
     2761 cattaattgc ggcatggtct caaacgggaa aaaaacaccg caaaggaata acatgggaat
     2821 actaagaaca agggaggata acatggctgt gttttccgat tctgcaaatg tcgctacagc
     2881 cataccgata ccaatgaaag ctgctgaata tacaaggata acaaggaaca atagtcccaa
     2941 ctggctggcc gaaatcaaga ccccataaaa aaggaaagca acaaccacca ggataatgcc
     3001 ctgcaacatg gctataagaa taagggcgga tgtttttgcg aaaatgaact ctcccaggga
     3061 taatggtgtt aaaagtgttc tcaagagggt ctttttagtt ctctcttgca cgatagtaat
     3121 tgaggacagc aggaatgaaa tgaacatcaa gacgatagat acgatcgcag gcatcaggaa
     3181 atcaaggtaa gacatctccc tgaatacagg agtcctttca agtttgatgg gagcggcaat
     3241 agctgcaggt gactttattc gtacttcgcc aagaatactt tttgtcctgt caatttctga
     3301 aaccaggtct tgagtgctat ttgtcatttc tgtggagagg ttgttcatct ggacggaaac
     3361 attggctgat cggactttga cctgatccag gattacggta gcagtttcga tatttgaaat
     3421 atttccccga atatttgaga tctcaaacgt gtaattgccc atcaattctt gcatgttctg
     3481 caatttgata tcggttcgtt caagtttttc atgcaactca gagccctttt gggcaacatt
     3541 agcaaggcca ttataaagtg atcttatacg gtcggtcttt tcaattaaaa gcacccttgt
     3601 atcctgtatt tgtcggatag tgtttgcgat ggatatgcat tgtggagtgg aaggaatggc
     3661 acatgttgaa ttatatgttc cctgaagatc agtccggatc gccaataatg cggcatcggt
     3721 agaatcaata ttatcgacca caagcttgag gtcatccctc aatgtcgcag aatcattata
     3781 gatcgaactt acattcttat caagttccaa aatgtcattt ctcgtagtat ttatatccac
     3841 agacgtttgg gaaaggtcga tcttcatctg gtcaagtgtg tttttcatca tattaattga
     3901 ttcattcatc ctggtcacat tgatctcatc cagtcggaca agtacatttt gtgtatcatt
     3961 gttcagtttg ccgatgtctt catttattga aaagacacct ttttttaatg gaccaagttc
     4021 cgattccatc tctcccaggt tttcccagag cttactgata aaacccagag taagttcata
     4081 tgacattttt tcagtagtag taaggaagac cgcagttata accggagcta tctgtactct
     4141 tgaattatca ataaacaatt tcatagactg tgtctgattt tcgggaatga aaatccccgc
     4201 gctgtatttt cctttcatga cgccatcttc gataactgac gggtcattag tgttctcttt
     4261 aacgattatg aacatcttgc tgttatttaa ttcttcgatt atgtcatccc ccatttcaga
     4321 cccaccccca acacccagtt ttacctcttt cagaccctgg tcagaggctc cggaaaacac
     4381 tgaaccgatc aatatcatta taagaatagg agcaagaaat atcaatatta atgttttcct
     4441 gtggcggaca tataccagga aatctttctt tatgacagga atgatgctca atgccactct
     4501 tctcccgaag catgaatgaa tacatcttcc agactggtag tgcttgattc tacagttatg
     4561 atcttttctt caagtgattc aaaaacatca aatattttat gaagctgtac cttccccgta
     4621 tgggttattt taagcccgtt atcgctaacg ctgcatgatg atatatttcc aagcgataac
     4681 acgttatctt tcagcacact gtagttcccg ggtgagcttg ttatcaggat catcttttcc
     4741 cccacctttt ttttcagttc gtcctgtttt ccttcgataa ctattttacc cctgttcatt
     4801 atcagtatcc tgtcgcacaa taagtccgct tcttccatat aatgtgtagt gagaaggatg
     4861 gtagtccctt catcgttaac aacctgaatg gtttcccaca gggcttttct tgatacaggg
     4921 tcaagcccta cgcttggttc atcaaggatc aatacctccg gtttatgtat gagcgcgcag
     4981 gcgatattta gccttgtttt cattccccct gacaggttat tggccctgat attacttttt
     5041 tcatcaagtt taagtaatgt aaggatttta tggcttcttt tcttaatttc aatt
//
LOCUS       DATGBN010000189         6054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_92267, whole genome shotgun sequence.
ACCESSION   DATGBN010000189 DATGBN010000000
VERSION     DATGBN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92267"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VKL21_04545"
     CDS             <1..194
                     /locus_tag="VKL21_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45071.1"
                     /translation="IILNRFNLTLLTFNYQLPRGELFYWKEVLVVISVIIIQILVYRW
                     IVNRMPVLRDHPDYPNDSH"
     gene            complement(445..783)
                     /locus_tag="VKL21_04550"
     CDS             complement(445..783)
                     /locus_tag="VKL21_04550"
                     /inference="COORDINATES: protein motif:HMM:NF021634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB45072.1"
                     /translation="MNSNIEFIAPEYTLTEILKHSDIIKKKSKLTSKDLQYVMDMLFS
                     RITIYPKDEYADYYTQAKEIMKDIDPDDAPFLALAMKTKVDGIWSEDKGFQQQNNVKI
                     YTTKELFEFI"
     gene            complement(859..1083)
                     /locus_tag="VKL21_04555"
     CDS             complement(859..1083)
                     /locus_tag="VKL21_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45073.1"
                     /translation="MPTMTLSVPDDLYKVIKQHDEVKWSVIARNAMWEYARKIQLVDS
                     ILEKSELTEKDAAEIGKLIKKSIREQHEIE"
     gene            complement(1254..1802)
                     /gene="trmY"
                     /locus_tag="VKL21_04560"
     CDS             complement(1254..1802)
                     /gene="trmY"
                     /locus_tag="VKL21_04560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HMB45074.1"
                     /translation="MREFILYSRTGRTDPRFPNLREGGRLDVVYQSILMSLFRSHAIR
                     RDVTFHALLGGPPNPPLELIVEGEKLRDARTDERTWEDILRRVLSGREHPGITLRKNS
                     LQRLVKEKTNIFVLEESGESINTIDLGSDPVFVLGDQVGLPKKDEDFVLRYGKKVSIG
                     EKAYLASACICIINYILDRREG"
     gene            complement(2040..2885)
                     /locus_tag="VKL21_04565"
     CDS             complement(2040..2885)
                     /locus_tag="VKL21_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMB45075.1"
                     /translation="MIERLKKLGLTSYESQAYVALIKLGDAEADEIALNAKIPMGRIY
                     NVLSSLEEAHLVSAQDTRPRRYACVDPATALTRLSNAKQEELMQASQEIQALAVDLTN
                     ELSGVVSRKTEKSFWTVAIGNESHELIREIISGSRKELLFFMASRMTSERIKKELLKD
                     DHVEIMTALYDAIKMGIEVKVILNNDVDFNRLEDLPIVEKMMGHIGKEFNCRLATIPA
                     TPFDIIDGENVLLQMLNPLNPEELFAVVNIRDAKLAEELRNKFFTIWDKAEVYCGMKN
                     RNSYI"
     gene            3005..4186
                     /locus_tag="VKL21_04570"
     CDS             3005..4186
                     /locus_tag="VKL21_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMB45076.1"
                     /translation="MADFLQIYLTRLAQIQKKHTKILAIAVILITIFLGIGLRDLTIN
                     SDFRKEMPKELPIFKLNDRITDEFGGQDSVVIAIELDDTVDSKTAVRDIRDPRVIQAL
                     ILLDEDLRGETSVTSVSSPASFFRGQKGVSPENITQTLRSNPMTDGFFSKNYKMALMF
                     VAADIGTGEEQIQNFDLLIQEHIKNTPKPTGVKFGITGAPIIRMTIFDLLKRDAILTL
                     IVSATIILLLLFVMEGSYTHGLLVFAPLSLGLIWTMGTLGWLGIPLSVATVGLSSMIL
                     GLGVEYGVFVLSRYKEERAKNINQLDSMKTTVYSIGTAVIGSGLTTIVGFGVLSFATV
                     PMIQHLGQTLALGIAYCLLAALFVNPVFILLEEDYEYWNTNRKFEKLSIRKEVHSQRV
                     R"
     gene            4188..5372
                     /locus_tag="VKL21_04575"
     CDS             4188..5372
                     /locus_tag="VKL21_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMB45077.1"
                     /translation="MIKPLEKYSEFITRHPFTVVVIMLLVSAYAIQMIGTIETKKSDM
                     KSMLPKDVASIATLNSIENEFGSTNFALFAVEIDPSYNGSDEVRDVREGRVLLYLNQL
                     TELAAHTDNVIDVTSATTVLKSINQGHLPRSEREVQELTVKNGLLDNYISKDYTLALV
                     KIQTTDDVDLKSLESELDKIINELSRPPGIRTTVGGAAMEGVVMEKNIGPDMAKTSMY
                     ALIGILIIILVLFRSIKYGFTPMTTIIFGTIWAMGFVGFIGMGMSSQTSGVLSMIMGI
                     GIDFGIQVVTRYRFELENIPGLKERHPNLDIGPRDAMAVTLNNVIIPMLTTTLAALIG
                     FQAMSLGKLTFLGDMGTIMSYGVAASMVAAITILPALVIIIDTTDIRKHMKFIKRFEV
                     RK"
     gene            5369..>6054
                     /locus_tag="VKL21_04580"
     CDS             5369..>6054
                     /locus_tag="VKL21_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45078.1"
                     /translation="MNRILKQIAITGIVFWLLLIIVNPVSSAPFTGAQGSRIQANFMN
                     QDPDPADAGKDVELRWQITNTIPGTIEVLKFHLDADYPFLFEAGDSPDKDLGASVGTN
                     DNKLYYVLHYKLRVADNALKGTYNITLNWNTGDGWTQKEFPIYIDPKRADFVVGALVT
                     SPEKIIADTKEAKLSVNIDNIGKGNAENVKVKILLPTGFKPTYSYSDEESPGIIQMGT
                     SKTTNFYVDV"
BASE COUNT         1700 a         1250 c         1279 g         1825 t
ORIGIN      
        1 ggataatcct taaccgcttc aatctgacac tgttaacctt taactaccag ctccctcgcg
       61 gggaactttt ctactggaag gaagtgctgg tagtcatatc ggttatcata atccagatac
      121 tggtatacag atggatcgtt aacaggatgc ctgtcctgcg ggatcatccc gattacccga
      181 acgatagtca ctaggtctat aggagaaagg gactggataa agccgtgtca taacggttct
      241 atcctgattt ttttttatca gttacctcgg aggcatccct acgaggatgc tcagggtttt
      301 gtacaccttt tgctatcatg gattctgatt ctcaaatctg gcgcataatg taatcttatg
      361 cgaatgctcg ataatttgtt tttcttttgg ttttttagat ttcaggatgc acgtcgaggc
      421 gcaggtttat ggtaaaaatt attcctaaat aaactcaaaa agttcctttg tcgtatatat
      481 ttttacatta ttctgttgct gaaatccttt atcttcgctc catatcccat caaccttagt
      541 tttcattgca agtgccaaga agggtgcatc atctggatct atatctttca tgatttcttt
      601 agcttgagtg tagtaatcag catattcatc ttttggatag attgtgattc gtgaaaataa
      661 catatccatc acgtattgaa ggtccttaga tgtgagtttt gacttctttt tgataatatc
      721 ggaatgcttt aagatttcag taagagtgta ttccggtgct ataaattcga tatttgaatt
      781 cattataacg tcgtgtggag ttagatatga gggaggatat aataatatta gtatctatta
      841 ccagtttcat ttcccatctc actcaatttc gtgctgttct cgtatggact tttttatgag
      901 tttccctatt tcagcagcat ctttttctgt caattcactt ttttccagta tgctgtccac
      961 taactgaatt tttcgggcat attcccacat tgcatttcgc gcaatgacac tccattttac
     1021 ttcatcatgt tgtttgatga ctttgtagag atcatccgga accgataaag tcattgttgg
     1081 cataggtata atatatgtgc aaacacatat ttatgtttat ccatgtacct gaatttcagc
     1141 atattaattg tttcttggat tctattgcca ttcgagagca tgataacgag cgcggagagc
     1201 agtcaccggt ataccccgga caaatagtac aaggcctgaa gctgttgatt tcatcaaccc
     1261 tctcttctat caagaatgta attgataatg caaatacagg ctgaggcaag atacgctttt
     1321 tccccgatcg agactttttt tccatacctc agcacgaaat cttcatcttt ttttggcaat
     1381 cccacctggt cacccaggac aaaaacaggg tcgcttccaa ggtctatggt gtttatactc
     1441 tctccggatt cttcaagcac aaatatgttg gttttctctt ttaccaggcg ctgaagacta
     1501 tttttcctga gggttatgcc ggggtgttcc ctgcctgaaa ggactcttcg taaaatgtcc
     1561 tcccatgttc tttcatctgt ccgggcatcc ctgagttttt ctccttcaac gatcagttca
     1621 agcggcgggt tgggcggccc tcccagcaat gcatggaatg tgacatctct tcgtatcgca
     1681 tgggagcgaa acagtgacat caaaatcgac tggtaaacca catcgagccg acctccctcc
     1741 ctcaggtttg ggaatcgtgg gtcggtcctt ccggttcttg aatataatat gaattcgcgc
     1801 attctcagat gtcttcccgg ggaatttgtg ccctctattg gtgttgatat ctcttctgat
     1861 ttaaaatact atatgttttt gctggtcatt caaattaaag accttttaat ttattttgga
     1921 gaacatgaaa aaaaatatcg agaaatatct agataaccag ttacgacgga caggttgggg
     1981 agtgggggtt agtgtatcta aaaaatgacc tgcccatcgc aaccctaaca atatcatggt
     2041 catatataag agttacgatt cttcattcca caatacactt ctgccttatc ccatattgta
     2101 aagaatttat tcctcaactc ttctgcaagt tttgcatccc ttatattaac caccgcaaaa
     2161 agttcctccg ggttaagtgg attcagcatc tgaagcaata cattttcccc gtcgattatg
     2221 tcaaaaggcg ttgcgggaat agttgcaagc ctgcaattga attcctttcc gatgtgtccc
     2281 atcattttct caactattgg taaatcttca agcctattga aatctacgtc attgttaaga
     2341 attactttaa cctcgatccc catttttata gcgtcataaa gagccgtcat aatttcaaca
     2401 tgatcatctt tcaatagctc tttttttatt ctctcggagg tcatcctcga agccatgaag
     2461 aacagtaatt cttttcgaga cccggatatt atctccctga taagttcatg ggactcattt
     2521 ccaatggcca ctgtccagaa gcttttttct gtttttctcg aaacaactcc tgagagttca
     2581 tttgtaaggt caacggcaag agcctggatt tcctgtgacg cctgcattaa ttcttcctgt
     2641 tttgcatttg aaagacgcgt tagcgcagta gcaggatcaa cgcatgcata ccgccgcggt
     2701 cttgtatcct gcgcactgac aagatgagct tcttcgaggc ttgaaagcac attatatatg
     2761 cggcccattg gtattttcgc atttagcgct atttcgtcag cctcggcatc tccgagttta
     2821 ataagggcaa cataagcctg tgattcataa cttgtaagtc cgagtttctt taatctttca
     2881 atcacagggg attatttatc atatagatat ttaaatgttt tcaatttaaa caactgatga
     2941 gttgtttaaa acacaatatt ttaatacctg tatttacaaa tagacgtaaa attcaggtga
     3001 ctaaatggct gatttccttc aaatctattt aacaagactt gcgcaaatac aaaaaaagca
     3061 caccaaaatt ttggcaatag cagtcattct tataacaata ttcctgggaa tcggccttcg
     3121 ggacctgacc ataaattcgg atttcagaaa ggaaatgcca aaagaacttc ccatcttcaa
     3181 acttaacgac cgcataactg atgaattcgg gggacaggat tctgtggtca ttgcgataga
     3241 gctggatgat acagtggatt caaaaactgc cgtaagggat attcgcgatc caagggtgat
     3301 acaggccctg atccttctgg atgaagattt aaggggagag acaagtgtaa caagcgtcag
     3361 ttcgcctgcg agttttttca gggggcaaaa aggggtttca cctgaaaata ttacacaaac
     3421 ccttcgcagc aatcccatga cagacggatt cttcagtaag aattataaga tggcgctaat
     3481 gtttgttgcc gccgatatag gaactgggga agagcagata cagaattttg atttgcttat
     3541 ccaggagcac atcaaaaaca ctccaaaacc cacaggtgtg aaatttggca taacgggggc
     3601 gccaattatc cggatgacca tttttgatct attaaaaaga gatgctatcc tgacacttat
     3661 tgtctctgct accataattc tgctcctgct atttgtaatg gagggctcat atacgcatgg
     3721 attgctggta tttgcacccc tctccctggg attgatatgg acaatgggaa cacttggctg
     3781 gctgggtata ccgctttctg tagcgacagt tggtctttcc tccatgatcc tgggattggg
     3841 agtagaatat ggggtattcg tgttgagcag gtataaagaa gaaagggcaa agaacataaa
     3901 ccagcttgat tcaatgaaga ccactgttta ttcgatcggt actgctgtca tcggttcagg
     3961 attgacaacc atcgttggct ttggggttct ttcatttgcg accgttccca tgatccagca
     4021 tcttggacag actcttgccc tgggtatagc ttattgtctc cttgccgctc tttttgtcaa
     4081 tcctgtattc attcttctgg aagaggacta tgaatactgg aatacaaaca ggaagtttga
     4141 gaaactttcc ataaggaaag aagtacattc gcagagggtg aggtagaatg ataaaacctc
     4201 ttgaaaaata ttcagagttc attaccagac atcccttcac tgttgttgtg atcatgcttc
     4261 tggtatccgc ttatgccatc cagatgatag gcacgatcga aacaaaaaaa tccgatatga
     4321 aatcaatgct tcccaaggat gtggcttcaa tagccactct gaacagcatt gaaaatgaat
     4381 tcggaagcac caacttcgct ttattcgctg tcgagatcga tccttcttat aatggatctg
     4441 atgaagtgcg cgatgtacgt gaagggcggg ttttgctcta tttgaaccag ttaacagagc
     4501 ttgcggcgca tactgataac gttattgatg taactagcgc tacaactgtc ctcaaaagca
     4561 taaatcaagg gcatctcccc cggtccgaaa gggaagtaca ggaacttaca gtcaagaacg
     4621 gtctgcttga caattatatc agcaaggatt ataccctggc tcttgtaaag atacaaacaa
     4681 ccgatgatgt agatctcaaa tcactggaaa gcgagcttga taagataata aatgaactat
     4741 cccggcctcc aggtataaga acaactgtcg ggggtgcggc aatggaagga gttgtcatgg
     4801 agaagaatat cgggcctgat atggcaaaga cctccatgta tgctcttata ggaatattga
     4861 taattattct ggtgctcttc cgttccataa aatatggatt caccccgatg accacgataa
     4921 tctttgggac gatctgggca atgggctttg tgggatttat cggtatgggc atgagttccc
     4981 agacatcggg ggtcttatct atgatcatgg gtattggaat tgatttcggg atacaggttg
     5041 taacaaggta caggtttgag ctggaaaata tcccgggttt gaaggaacgc cacccgaatc
     5101 tggatattgg accgagggat gccatggcag tgaccctgaa caacgtaata atccccatgt
     5161 taacaactac acttgccgca ctgataggtt tccaggcaat gtctcttgga aaacttacct
     5221 tccttggcga tatgggaacg atcatgagct acggtgtggc cgcgagtatg gttgcagcca
     5281 taacaatact ccccgcgctt gtgatcataa tagatacaac tgatattagg aaacatatga
     5341 aatttattaa aagatttgag gtacgaaaat gaatagaatt ttaaagcaga ttgcaataac
     5401 cggaatagtt ttttggctat tgctgataat agtaaaccct gtttcctctg ctccgtttac
     5461 aggcgcgcag ggaagcagga tacaggcaaa tttcatgaac caggatcctg atcctgcgga
     5521 tgcgggaaaa gatgtggaat taagatggca gataacaaac accatacctg gaaccattga
     5581 ggtcttgaaa ttccacctgg atgccgatta ccccttctta tttgaagccg gggattctcc
     5641 tgataaggat ctcggggcat ctgtgggaac aaatgataac aaattatatt atgtcctgca
     5701 ttataaatta agagttgcag ataacgcact caaaggaacg tataatatta cacttaactg
     5761 gaatacggga gacggttgga cgcagaaaga gttccccatc tatatcgacc ccaagagagc
     5821 tgatttcgtg gttggggcgc ttgtaacctc tccggaaaag atcatcgcgg ataccaagga
     5881 agcaaaatta tcagtaaata ttgataatat cggaaaaggc aatgcagaga acgttaaggt
     5941 caagattttg ttaccaacgg gtttcaagcc cacatacagt tattcggatg aggaaagccc
     6001 cggaattatc cagatgggca caagcaagac cacgaatttc tatgttgatg ttga
//
LOCUS       DATGBN010000190         1845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_441927, whole genome shotgun sequence.
ACCESSION   DATGBN010000190 DATGBN010000000
VERSION     DATGBN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441927"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VKL21_04585"
     CDS             complement(<1..976)
                     /locus_tag="VKL21_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HMB45079.1"
                     /translation="MTDKIDIPRILIAGDRSSAGKTTISIGIMSVLKDMGYTVQPYKV
                     GLDFIDPSYHTEVTGRYSRNLDGYLMSEDAVCEVYSHAASGADITVIEGVRGLFEGLE
                     ATSDIGSTAQIAKILKCPVILAINARSITRSTAALVSGYKSFDPEVNIVGVILNNIGS
                     QRHAQKAKTAIETYTDTPVIGVIPRNDSMKISMRHLGLIPAMEGRRRVADFDANLGRI
                     KEIIKEGIDIDRFISIANSAPQLEKPKENIFRTQPRENTIKIGVALDEAFNFYYRDNL
                     ELLELSGAELVYFSPVKDSTLPNVDGLYIGGGYPELFTNELEDNASMRE"
     gene            complement(1073..>1845)
                     /gene="cfbC"
                     /locus_tag="VKL21_04590"
     CDS             complement(1073..>1845)
                     /gene="cfbC"
                     /locus_tag="VKL21_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HMB45080.1"
                     /translation="YGKGGIGKSSTASNVAAACADEGYRVTIIGCDPKSDSSITLLRG
                     RRIPTIMDLMREGIDIKEEDIVFNGYKGVKCVEIGGPEPGIGCAGRGIIVAISLLKKI
                     SRAIEDTDLVIYDVPGDIVCGGFVAPVKKGLVNEAYVLTSGEYMPLYAANNICKGLSR
                     LEMTLNGVICNSRGALNEEKIVSEFSKEIGSQLLAFIPKDVLVQTCERDGFSVIENAP
                     DSEIAAVYRKLARSIMGKRDGKIPVPLDDKRLRELTRI"
BASE COUNT          472 a          470 c          351 g          552 t
ORIGIN      
        1 cttcccgcat ggaagcatta tcttcaagtt catttgtaaa taattcaggg taacctccac
       61 cgatatataa accatctaca ttaggcaggg tactatcttt cacaggactg aaataaacaa
      121 gttcggctcc tgaaagttca agaagttcaa ggttatcacg ataatagaaa ttaaatgcct
      181 catcaagcgc cacaccaatt tttatcgtgt tttcccgggg ttgggtccta aaaatattct
      241 ctttcggttt ttcaagctgt ggagcagaat tggcaatcga aatgaacctg tcgatatcga
      301 tgccttcttt tataatttct tttattcttc ccaggtttgc atcaaaatcg gccactcttc
      361 gtcgtccttc catcgcagga ataagcccga gatgcctcat cgagattttc atggagtcat
      421 tgcgaggtat caccccgata acaggagtat ccgtatatgt ctcaatcgca gtctttgctt
      481 tctgtgcatg tcgctggctt ccgatattat tcaggatgac gccaactatg ttcacttcag
      541 ggtcaaacga tttatatcct gaaacaagcg ctgccgtact tctggtaatg cttcttgcat
      601 tgatcgcaag aataacaggg cattttaata tttttgctat ctgggcagtg cttccgatat
      661 cacttgttgc ttcaagccct tcaaaaagac cccgtacgcc ttctataacg gtaatatcag
      721 ctccacttgc agcatgtgaa tatacttcac atacggcatc ttctgacatc agataaccat
      781 caaggttcct tgaatacctg cctgtaactt ccgtatgata actgggatca atgaaatcaa
      841 gccctacctt ataaggctgc acagtatacc ccatatcttt gagtactgac attatcccga
      901 tggatatggt ggtcttcccg gctgaagaac ggtcgcctgc aattagaatg cgggggatgt
      961 caatcttgtc ggtcatgtct tatccaccca cacaaagtgt tcaataccag attaacaaat
     1021 ctgcgttcat ctgcgtttat ctgcggtttc atttatctat tctcccacaa tcctaaatcc
     1081 gcgtcaattc cctcaacctt ttatcatcaa gcggcacagg tattttcccg tcccgttttc
     1141 ccataatgct cctggcaagc ttacggtaga ccgctgcaat ttccgaatcg ggagcattct
     1201 caataactga aaaaccatct ctttcacatg tctgcacaag aacgtctttt ggaataaaag
     1261 caagaagctg gctgccaatt tcttttgaga attcgctgac gattttttcc tcattcaaag
     1321 cgccgcgtga attgcatatt acgccgttaa gtgtcatttc cagacgcgaa aggcctttgc
     1381 agatgttgtt agctgcatac agcggcatgt attcgccgga agtgagtacg tatgcctcat
     1441 tcacaagccc tttttttaca ggggcaacaa aacccccgca tacgatatca ccgggcacat
     1501 cgtatattac aaggtctgta tcctcaatgg cgcgtgagat cttcttgagg aggcttattg
     1561 ccacgataat tcctctgcct gcacacccaa taccgggttc gggcccccct atctcaacac
     1621 atttgacccc tttgtatcca ttaaatacta tgtcttcttc cttgatatct ataccttccc
     1681 tcataaggtc cattattgtt ggaatgcgcc ttccccggag cagggtgatc gaagaatcac
     1741 tcttgggatc gcatcctatg atcgtaaccc ggtagccttc atcagcacat gctgccgcaa
     1801 cattggaagc agtgcttgat ttgccaatgc ccccttttcc gtaaa
//
LOCUS       DATGBN010000191         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_403012, whole genome shotgun sequence.
ACCESSION   DATGBN010000191 DATGBN010000000
VERSION     DATGBN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403012"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VKL21_04595"
     CDS             complement(<1..936)
                     /locus_tag="VKL21_04595"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMB45081.1"
                     /translation="MNDIFDNARKALEFAKKEGADEVEIYCVKGRSISIDIHRDVIDL
                     AKESLISGIGIRAIVKGAVGFSSTNDVFRIKEASVLAVKSARVRGSDPEWSGLPEKKK
                     QPAVKGIFDKKIADIEIESCIDHTCEMINGAKSIPTIFPTSGHFACGNSTKLILNSNG
                     IEIEEEDTIVSASIDAITEDAPLSTASEFDMSRKLDVDFYKLGERASTLALRSQHGIS
                     TQTRDCSVLLEPMAIADLLENTIVTSVNADNVQKGRSSLINKMGTSIASDQLSIIDDG
                     IFAGGLGTSSCDEEGTPSKRTPIIKEGILSSYIYDC"
     gene            complement(1206..>1596)
                     /locus_tag="VKL21_04600"
     CDS             complement(1206..>1596)
                     /locus_tag="VKL21_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45082.1"
                     /translation="LIIIGFFISIPFTPLVIIEAFLMLLLISIAMVSIGLVIGANLKS
                     PEGFSLVINFVMWPMFFFSGALFPVSNLAPWLSAITFINPLTYGVDALRGIILGINDF
                     PIMLDAAVLMLFAAIMIGIGVASFKRM"
BASE COUNT          484 a          336 c          319 g          457 t
ORIGIN      
        1 gcagtcataa atgtaagatg ataagatgcc ctctttaatg atgggtgttc tcttagatgg
       61 agtcccttct tcatcacatg aggaggtacc aagaccaccg gcaaaaattc catcatcgat
      121 aattgaaagt tgatctgagg ctatcgaagt acccattttg tttatcaaag atgatcttcc
      181 tttctggaca ttatccgcat ttaccgaggt cacgatagta ttttcaagaa gatctgcaat
      241 agccattggt tcaagaagca cactgcaatc ccgcgtttgc gtgcttatgc cgtgttgcga
      301 ccgcaaggcg agggtggagg ctctctcccc cagcttatag aaatccacat ccagctttcg
      361 cgacatgtca aattcagaag cggttgaaag aggcgcatcc tctgtaatag catctattga
      421 ggccgaaacg atagtatctt cttcctctat ctcgattccg tttgaattca agatgagttt
      481 tgttgaatta ccgcatgcaa aatgtcctga tgtggggaaa attgtgggga tagactttgc
      541 tccattgatc atttcgcaag tatgatcaat gcatgactca atctcgatat ccgcaatttt
      601 cttgtcaaaa atacctttaa ctgcgggttg tttctttttt tcaggaagcc cggaccattc
      661 agggtcactg cctctcactc gtgcggattt tacagcaaga acagaagctt ctttgatgcg
      721 aaatacatcg ttagtgctgg aaaaacccac cgccccttta acgatcgccc ttataccgat
      781 tcctgaaata agactttctt ttgcaagatc gatgacatcc ctgtgaatat ctattgaaat
      841 agacctgcct tttacacaat aaatctcaac ttcatccgct ccttcctttt ttgcaaactc
      901 aagagccttg cgggcattat caaaaatatc attcatggaa atcactgatt taaactaaag
      961 tatgggaata ctaaataata tgaaattgca ataattataa ttcatatttt gactcattta
     1021 attacggttt tatatcttat atagataagg ggtttgaaaa taaatataag aattataatt
     1081 attatgtgga aaatccattt ttctatccca aaggaacatc acagacctgc atatgcgggt
     1141 gtaaagtacc aaaagacctg tcagtgagga tacacgattg tccgtcgtgt gggttggtac
     1201 tcacttcaca ttcgcttaaa gctcgcaacg cctataccta tcatgatggc ggcgaaaagc
     1261 atcaaaacag ccgcatctaa catgatggga aaatcgttta tacccagaat gataccgcgc
     1321 aatgcgtcaa ccccatatgt caggggatta ataaatgtga tagcagagag ccagggtgca
     1381 agattgctga cagggaaaag tgcgccgctg aagaaaaaca ttggccacat tacaaaattt
     1441 atcacaaggc tgaatccttc ggggctcttg aggttcgcac caatgacgag tccaatactc
     1501 accattgcta ttgagataag gagaagcatc aggaacgctt ctattatcac aagaggagtg
     1561 aatgggatgc ttatgaaaaa tcctataatt ataaga
//
LOCUS       DATGBN010000192         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_325383, whole genome shotgun sequence.
ACCESSION   DATGBN010000192 DATGBN010000000
VERSION     DATGBN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325383"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VKL21_04605"
     CDS             complement(<1..257)
                     /locus_tag="VKL21_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HMB45083.1"
                     /translation="MVIYDEKQQEIAEKINPKSRISNWKDWKWQLKNSIKDISTFEKL
                     TGIRFEKNERNLLEKTIEKFPLSITPYYLSLIDAADYKNDP"
     gene            complement(258..1355)
                     /locus_tag="VKL21_04610"
     CDS             complement(258..1355)
                     /locus_tag="VKL21_04610"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMB45084.1"
                     /translation="MTLISEILNQNLFGVSIIKLGTFFIIIFITFIVKSIFFYIVDKK
                     IAILLRRTKPELHDLLVNAIKNPTGYLILLQGFYLAILSLQLPEKIGQFQIASIVRPI
                     FKLSIAFILLYFIFKIIDIIAYYLYKEAKKSESALDDQLVPLLVRSLRILVTTVGILF
                     ILQNFGYNVTSLIAGLGLGGLAFALAAQNTVSNLFGSITIFSDKPFQLGDWIQVGDIE
                     GTVEDVGFRTTRVRRFDQALVTVPNSQFINTGVINYSAMKKRRISFNLALTYGTSAGK
                     INEVVAGIKKIIEENEKFDHSYYIVRFTEFGAYSLNIFIYCFASTTDWAESLRIKEEF
                     NLKIMQLLEELGVEIAFPPQTLYLNSIKGGN"
     gene            complement(1463..>1913)
                     /locus_tag="VKL21_04615"
     CDS             complement(1463..>1913)
                     /locus_tag="VKL21_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB45085.1"
                     /translation="IELSGKVVFHCIFHDITEQKQAVEAVRESERRSREMLQNIQLIG
                     IMLDTNDNIIFCNDFLLKFTGWHREEVSGQNWFKMFVPQERQEESRLVFHQLLARGAP
                     FQFETEILTKRGDLHLISFNNVVLRNLQGSITGIASIGEDITLRRQA"
BASE COUNT          565 a          371 c          356 g          621 t
ORIGIN      
        1 aatggatcgt tcttgtaatc cgcagcatcg atcaaagata aataataagg cgttatggat
       61 agaggaaact tttcaatagt tttttcaaga aggttccttt catttttttc aaatctgata
      121 ccagttagtt tctcaaaagt ggaaatgtcc ttaatagaat tctttaattg ccacttccaa
      181 tctttccaat ttgatatcct ggatttcgga tttatctttt ctgctatctc ctgttgtttt
      241 tcatcataaa taaccattta atttccccct tttattgaat ttaaatataa tgtctgtggc
      301 gggaaggcga tttcaacacc cagctcttca aggagttgca tgatctttag attgaattct
      361 tcctttatcc tgagggactc agcccaatcc gtggtactgg caaagcagta aataaaaatg
      421 tttaacgaat atgcgccgaa ttccgtgaac cttactatgt aatatgaatg atcaaatttt
      481 tcattttctt caatgatctt ttttatgcct gctaccactt catttatctt gcctgcagaa
      541 gtcccatatg ttaaggcgag attgaaagat atcctgcgtt ttttcatcgc cgaataattg
      601 ataactcctg tattgatgaa ctggctatta gggaccgtaa ccagggcctg gtcaaacctt
      661 ctgacccttg tggttctgaa tcccacgtct tcgaccgtgc cctcaatgtc cccgacctgt
      721 atccaatccc caagctggaa aggtttatca gagaatatag taatggaacc aaaaaggttg
      781 cttacggtat tctgcgcggc aagtgcaaag gcaagtcccc ctagaccaag tcctgcgatc
      841 agggatgtta cgttgtaacc gaaattctgg aggatgaaca atattcctac tgttgtgacc
      901 agtatacgaa ggcttcttac aagcaaaggg acaagctgat catcaagagc gctttcactt
      961 tttttcgctt ctttatacag gtaatacgct atgatatcta taattttaaa gataaaatat
     1021 agtaatataa aggctattga aagtttgaaa atagggcgaa caattgatgc aatttggaat
     1081 tgcccgatct tttcaggtaa ttgtagagaa agtatggcta aatagaaacc ctggagcaat
     1141 atcaggtaac ctgttggatt ttttattgca tttaccagca ggtcgtgaag ttcgggtttt
     1201 gttcttctta aaagaatggc tattttctta tctacaatat aaaaaaaaat agattttact
     1261 ataaaagtaa taaatattat aataaaaaat gttccaagtt ttataataga tacaccaaat
     1321 aaattctggt taagtatttc tgaaattaat gtcatatgta tttatttgtt aagttacaga
     1381 tttaagtata aaagaaggat aagtattatc tattataata ctttcgggtt atccctttat
     1441 aagcctccgt gctactgtaa gattaagcct gtctgcgcaa tgtgatatcc tctccaatgc
     1501 tggctatgcc tgtaatgctg ccctggagat tgcgcaagac cacgttgttg aacgagatca
     1561 aatgcaggtc tcctctcttt gtcaaaattt cggtttcgaa ttggaaaggc gcaccccggg
     1621 caagcagctg gtggaatacc aacctgcttt cctcctgacg ttcctgtgga acaaacattt
     1681 taaaccagtt ctgtccagag acttcttcac ggtgccagcc agtgaatttg agcagaaaat
     1741 cattacaaaa tattatattg tcatttgtat ccagcataat accgataagc tggatattct
     1801 gtaacatctc gcgggatcgg cgctcagatt cacgaaccgc ttccacagcc tgtttttgct
     1861 cggtgatatc atggaatatg cagtggaaaa ccactttacc ggataactcg att
//
LOCUS       DATGBN010000193         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_364491, whole genome shotgun sequence.
ACCESSION   DATGBN010000193 DATGBN010000000
VERSION     DATGBN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364491"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VKL21_04620"
     CDS             <1..914
                     /locus_tag="VKL21_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMB45086.1"
                     /translation="APCFVNAHTHLGDSVIKDPPFLPLAQLVQPPHGLKHRILRKTSH
                     VELVSSMKASLNDMIKTGTCAFCDFREGGLAGVQALHEALGADPRIKSLVMGRPDKDM
                     DYLDSCDGTGLSSMNDLELELVRDIVRQTRGKRKRFAIHAGERDLTDISSALELQPDF
                     LIHLTNADEKHIKDIADSNIPVAVCPRSNYITRSGIPPIGKMLDAGILVSVGTDNVML
                     NSVNMFSEMEFLAKTNLYDDRQVFKLCTLNGAKLLGLDKESGSIKKGKKARLMILNKK
                     SDNMQGIKNPLSSLVRRARPDDIINII"
     gene            934..1380
                     /locus_tag="VKL21_04625"
     CDS             934..1380
                     /locus_tag="VKL21_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMB45087.1"
                     /translation="MASKFYEKILIATDGSEYTKNAVDYGIDIAKSTGAKLFAIYVVD
                     TAAFALIPMDAAWESMYELLKQEGDLAVKYVAERAEAEGLKVEGNLIEGHPADEIIKY
                     SEKNSISLIVMGTLGKSGLDRFLLGSVAEKVVRNSKIPILVVRGKK"
     gene            1399..2259
                     /locus_tag="VKL21_04630"
     CDS             1399..2259
                     /locus_tag="VKL21_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB45088.1"
                     /translation="MPNTPQGIKVDDVMARDVASAKLPGSRDEVLEILKSKHISGIPI
                     VKGGELVGIVTRTDLLKHPEEEQIAILMTRNPITITPEKSIVDAARLILENNIRRLPV
                     VEDGFLIGLVTIADIVGAIGRLNITSHISDYIEDGVFSVWSETPLCVVGSIMELADVK
                     AVPILDSTLTLVGVAADKDLISASIIEDKTEMSNMSAGSDDDAWTWESMRDTMSLYYS
                     VSKIQLPNSPVKEIVKLTGEPETASLSLSVSECARKMRRQRIDQLPIINSSKKLLGLV
                     RDKDLLKAIV"
     gene            2263..>3079
                     /locus_tag="VKL21_04635"
     CDS             2263..>3079
                     /locus_tag="VKL21_04635"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMB45089.1"
                     /translation="MRSDNIKKGLERAPHRSLLKAVGLTDEEMSLPFIGVVNSWNEII
                     PGHIHLDKLAEAVKAGIRMAGGVPFEFNTIGICDGIAMGHTGMKNSLPSREIIADSIE
                     LMVEAHQFDGMVMIPTCDKIVPGHLMAAGRLDIPTIVVTGGPMMPGIVGDEPRDLISL
                     FEAVGARQNGNISDMELKNLEDCSCCGAGSCAGLFTANTMACVTEALGLSLPGCGTAH
                     AVDAKKVRIAKHSGMKILELVEKGITARSIVTEKSLDNAIRIDMAIGGSTNTAL"
BASE COUNT          950 a          566 c          777 g          786 t
ORIGIN      
        1 tagccccatg ctttgtgaat gcgcatactc accttggaga ttcggtaata aaggatcccc
       61 catttttacc cttggcgcaa ctcgtgcaac cccctcatgg tttaaaacac aggattttga
      121 ggaaaacctc gcatgttgaa cttgtttctt ccatgaaggc aagtcttaat gatatgataa
      181 aaacaggaac ttgcgctttt tgcgatttcc gtgaaggagg gcttgccgga gtacaggcgc
      241 tccatgaggc attgggggcc gatccccgga tcaaatctct ggttatgggg cgcccggata
      301 aggatatgga ttatcttgac tcttgcgatg ggacaggact tagcagcatg aacgacctgg
      361 aacttgaatt ggtcagggat attgttagac aaacccgggg gaaaagaaag agatttgcca
      421 tccatgcagg tgaaagggac ttaactgata tttcaagcgc tcttgaatta caacctgatt
      481 tcctgataca tcttacaaat gccgatgaaa aacatattaa ggatattgcg gattcaaata
      541 ttcctgtggc ggtctgccca cgctcgaatt atatcacaag atcaggaatc ccgcctatcg
      601 ggaaaatgct tgatgcagga attttggttt ctgttggcac tgataatgta atgcttaatt
      661 ccgtcaatat gttttctgag atggaatttc ttgcaaaaac aaacctatat gatgacagac
      721 aagtatttaa actgtgcacg ctaaatggag caaaactgtt aggcttagat aaagaatcag
      781 gttccataaa aaagggtaaa aaagcaagat tgatgatctt gaataaaaaa tccgataata
      841 tgcagggaat aaaaaatcca ttgagcagtt tagtcaggcg ggctagaccg gatgatatta
      901 taaatataat ttaaagtgtg aaggaggaaa attatggcca gtaaattcta tgaaaaaata
      961 ttaattgcaa cagatggttc ggaatacaca aaaaatgcag ttgattacgg tatcgacatt
     1021 gcaaagagca cgggagcgaa gctgtttgca atttatgtag ttgacactgc tgcttttgct
     1081 ttgatcccca tggatgctgc gtgggagagc atgtatgaac ttctaaaaca ggaaggagac
     1141 ctggctgtaa aatatgttgc ggaaagagcg gaagcagaag ggctaaaagt tgaagggaat
     1201 ttaattgaag ggcatcctgc tgatgaaata ataaaatatt ccgaaaaaaa ctcaatttct
     1261 ctcatagtaa tgggaaccct gggaaagagc gggcttgaca gattccttct gggaagcgtt
     1321 gctgagaaag tagtccgtaa ttctaaaata cctatactag tagtccgtgg taaaaagtaa
     1381 taagagagga gatataaaat gccgaatacg ccgcagggca taaaagtgga tgatgtaatg
     1441 gccagggacg ttgccagcgc caaattaccc ggatcaaggg atgaagttct tgaaatattg
     1501 aagtcaaagc atatttcggg aattccgatt gtaaaaggtg gagaactggt aggcatcgta
     1561 acaaggacag atttattgaa acatcccgaa gaggagcaga tcgcgatcct gatgacgaga
     1621 aatcccatca cgatcacgcc tgaaaaatcg attgtggatg ctgcaagatt gatcctggaa
     1681 aataatatcc ggcgtttacc agtggtggaa gatggtttcc ttatcgggct tgttacaata
     1741 gcagatattg tgggggcaat cggaaggctg aatatcactt ctcatatcag tgattatatt
     1801 gaagatggcg tgttttcagt ctggagtgaa acacctcttt gcgtggttgg ctcgatcatg
     1861 gagcttgccg atgttaaagc agttccgata ttggattcga cacttacact tgtaggtgta
     1921 gctgctgata aggatcttat aagcgcaagc ataattgagg ataaaacaga gatgtcaaat
     1981 atgtctgctg gttctgatga cgatgcatgg acatgggaat ccatgcgtga cactatgagt
     2041 ctttattaca gcgtttccaa gatccagctc cctaattcgc ctgtgaaaga aatagtaaag
     2101 ctaacaggtg aacctgagac tgcatcattg agcttgtcgg taagtgaatg cgcgcgtaaa
     2161 atgagacgtc agagaattga tcagctgccg attatcaaca gctcaaaaaa actactgggt
     2221 ttggtacggg ataaagacct tttgaaggcg attgtttaat tcatgagaag cgataatata
     2281 aaaaaaggac ttgaacgggc gccacacagg tctttattga aggcagtcgg gctgacagat
     2341 gaggaaatgt cccttccttt tatcggagtt gtgaattcct ggaatgagat aatacccgga
     2401 catatccatc ttgataagct tgctgaggct gtaaaagccg gaattcgcat ggctggcggc
     2461 gttccgtttg aatttaacac tatcgggata tgcgatggca tagcaatggg tcataccggt
     2521 atgaagaatt ccctcccaag cagggaaata atagcggata gcatagaact gatggtggaa
     2581 gcccaccagt tcgatggaat ggtaatgata ccgacatgcg ataagatcgt gccgggacat
     2641 ctcatggctg caggccgtct tgatatacct accatagttg taacaggcgg gcctatgatg
     2701 cctgggatcg tgggtgatga gccccgtgat ttaatatcat tgttcgaggc cgtaggggcg
     2761 cgccagaacg gaaatatctc agacatggaa cttaagaatc ttgaagactg ttcctgttgc
     2821 ggagcaggtt catgcgcagg tctgttcact gccaatacca tggcatgtgt tactgaagcg
     2881 cttggcctga gccttccagg atgcgggaca gcgcatgctg tagatgcaaa aaaagtaagg
     2941 atcgctaagc actccggtat gaagatactt gaacttgttg aaaaaggaat aacagcaaga
     3001 tcaatagtca ctgagaagtc acttgacaat gcaatacgaa ttgacatggc aataggggga
     3061 agcacaaaca cagctctgc
//
LOCUS       DATGBN010000194         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_442028, whole genome shotgun sequence.
ACCESSION   DATGBN010000194 DATGBN010000000
VERSION     DATGBN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442028"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..211)
                     /locus_tag="VKL21_04640"
     CDS             complement(71..211)
                     /locus_tag="VKL21_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45090.1"
                     /translation="MWQIIREQKQREARTNQMLWEFNLYKEQERIKVKQRENEAMNIM
                     YA"
     gene            complement(305..2122)
                     /locus_tag="VKL21_04645"
     CDS             complement(305..2122)
                     /locus_tag="VKL21_04645"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HMB45091.1"
                     /translation="MVMQLLSHSKKNAKGKTYTYYSIAEPYWENNKNKKRDLFYLGSL
                     TPSQAQQIRNILKATQSEDTFTATFEDLLFENHYRYLDIAFLNHLWDQEWKGLSSIFP
                     SPEETSNTRQKEISTADIAKLLTFYRCLDPGSYLSAVEWFETTTCNILLDMEASQINE
                     SRFYRELTAIENQKEKIEQWLYQTLKNRNEKSMRIVFYDLTDSYFEGRKCPIAKPGRT
                     KANGFREKRIVLSLLVNSEGYPFSWKILEDYTADVSTLTKNTDLWQKQFNFSKIILVF
                     DRGMVSDENLLNLENKQSYLYITALDKPQIAGVEKVNLDRFKDFTNETTEKEILSKGL
                     TKYDDITFYEDIGIDSASRRHILIFNSDLLKDQRKTREKLIDIAIMELEEEKKLLLNA
                     KKSRNLKPTEYKINEILRRRGVQSFIDPKLESINISGDRGSSINSFNLTYEINVEAIK
                     KTMLVDGIWMLVTNITETLESEEDRLKPEEIINAYRDKNRIEEAFRNVKSFIKFQPTF
                     VYTDDHVRAHYTICILSYLLDVTVTNRLRQKPIEEIGSVEKVHRILRRCEVGKLSVKG
                     SDRTGLKLMPITGEQKSVLKLFDCNYIVKGEFLKSLGIK"
     gene            complement(2156..>3309)
                     /locus_tag="VKL21_04650"
     CDS             complement(2156..>3309)
                     /locus_tag="VKL21_04650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HMB45092.1"
                     /translation="KLKADPNNIAANLNNYINGFSSNAREIIEYFNFTEHIARLDDVN
                     LLFMIVKRVGEIDLHPDKVSNLEMGYIFEELIRRFSELSNETAGEHFTPREVIRLMVN
                     LLFVNDREILTKEGIVRTLYDPVSGTGGMLSISEEYLRELNPDARLEVFGQEVNPESY
                     AICKSDMLIKGQNTSNIKFGNSFTNDGLVGETFDYMLSNPPFGVEWKKVQKQIDDERE
                     KRGFAGRFGAGLPRINDGSFLFLQHMISKMKPANGGSRIGIVFNGSPLFTGGTGSGES
                     DIRKWIIENDMLEAIVAMPDQLFYNKGIFTYIWIVTNRKARERKGKIQLVNAVSFYNK
                     THLLQISVKSESFLKVNNTPSNPFLLVLHTYYNMLLYILRTDFLTIKYL"
BASE COUNT          878 a          649 c          563 g         1219 t
ORIGIN      
        1 ccccttctat ttggggtaat cttaccggga tgtgcttcaa cagcaatggg agttcttaaa
       61 ttgtttatat tcatgcatac ataatattca tagcttcgtt ttccctttgt ttaactttga
      121 tacgttcttg ttccttgtac agattgaatt cccatagcat ctggtttgtt ctggcttctc
      181 tttgtttttg ttctcttatt atttgccaca tcttttcttg tttccttctt atcacccatc
      241 ttcgtcagat tcaaaccaat atccatatcg gtagagcacc ctatgtttaa aattctgttc
      301 ttaatcactt aatgcctagt gattttaaaa attccccctt cacaatataa ttacaatcaa
      361 atagctttaa cacactcttt tgctcacccg ttataggcat gagtttcagc ccagtgcggt
      421 cagatccttt cacactcaat tttccaactt cacatctcct cagaatcctg tgaacttttt
      481 ccacgcttcc tatttcttca atcggttttt gtctgagcct atttgtaact gtcacatcca
      541 gcaagtatga caagatgcaa atcgtataat gtgcacgaac atggtcatct gtatatacaa
      601 atgtcggctg aaattttatg aacgatttta cattccgaaa agcttcctca atcctgtttt
      661 tatctcgata agcatttatg atctcttccg gtttaagtct gtcctcttca gattccaggg
      721 tttctgtaat gtttgttacc agcatccaaa taccatctac cagcattgtt ttttttatag
      781 cttcaacatt tatttcatat gttaaattaa agctgttgat tgatgatcca cgatctccag
      841 atatattgat actttcaagt ttggggtcaa tgaaagattg aacccctcgt ctcctaagta
      901 tttcgttaat tttatattct gtaggcttaa gatttctgct ttttttcgca ttcagtagca
      961 attttttctc ttcttccaat tccattattg ctatgtcgat tagcttttct cttgtttttc
     1021 gctgatcctt gaggagatcc gaattgaaga taaggatatg tcgtctactg gcactatcga
     1081 ttccaatatc ttcatagaaa gttatatcat catattttgt caatcctttt gataaaatct
     1141 ctttttcagt agtctcattt gtgaaatcct taaacctgtc aagatttacc ttttcaaccc
     1201 ctgcgatctg tggtttatca agagctgtga tataaaggta gctctgttta ttctccagat
     1261 ttaggagatt ttcatccgag accataccac ggtcaaaaac taatattatt tttgaaaagt
     1321 tgaattgctt ttgccataaa tcagtatttt tcgtgagtgt tgagacatcg gcagtgtagt
     1381 cttcaagtat tttccaggaa aaggggtaac cttctgaatt aacaagcaga gaaagaacga
     1441 ttcttttttc tcggaagcca ttggctttag ttcttccggg ttttgcgatt ggacattttc
     1501 gtccttcgaa atatgaatcc gtgagatcat aaaaaacaat tcgcatgctt ttctcatttc
     1561 ggtttttgag tgtttggtaa agccattgct cgatcttttc cttttggttt tcgattgctg
     1621 tgagttcacg gtaaaatctg gattcattga tctgactggc ctccatatca agaagaatgt
     1681 tacaggtggt tgtttcaaac cattctacgg ctgataaata acttcctggg tctaagcatc
     1741 gatagaaagt aaggagtttt gcgatgtctg ccgttgatat ttctttttgt ctggtattgc
     1801 tggtttcttc aggtgatgga aaaatgcttg aaagtccttt ccattcttga tcccacagat
     1861 ggttcagaaa tgcaatgtca agataacgat agtggttttc gaatagcagg tcttcaaaag
     1921 ttgcagtgaa ggtgtcctca gattgagtgg ctttcaggat attacggatt tgttgtgctt
     1981 gtgagggtgt gaggcttccc agatagaata aatctctttt tttgtttttg ttgttttccc
     2041 aataaggttc ggcaatgctg taatatgtgt aggttttacc ttttgcgttc ttttttgaat
     2101 ggcttaaaag ttgcattacc ataatctgtt cttaatatac tatattaaac gccatctata
     2161 aatattttat tgttaaaaaa tctgttctta atatgtataa taacatatta taatatgtgt
     2221 gcaagaccaa taggaatgga tttgatggag tattatttac ttttaaaaat gattcggatt
     2281 taacggagat ctggagaaga tgggttttat tgtaaaagct gaccgcattt actaattgga
     2341 ttttcccttt tctctctctg gcttttctgt tggttactat ccagatataa gtgaaaatac
     2401 ccttattata aaataattgg tcaggcattg caacaatggc ttcaagcata tcattttcaa
     2461 tgatccattt tcggatgtcg ctttccccgc ttccagtacc gcctgtaaac aaaggcgacc
     2521 cattaaaaac aattcctatt cttgagccgc catttgcagg cttcatttta gaaatcatat
     2581 gctgcaaaaa taagaaagag ccatcgttta ttcttggtaa tccagcgccg aaacggccag
     2641 caaaacctct tttctcgcgc tcgtcatcga tttgtttttg aactttcttc cactcaacgc
     2701 caaatggtgg attcgaaagc atataatcaa aagtttcacc caccaaacca tcattagtaa
     2761 atgagtttcc aaattttatg tttgaagtgt tttgcccttt gatcagcata tccgatttac
     2821 agattgcgta agactcagga ttgacttcct gcccgaatac ttcaagcctt gcatcaggat
     2881 taagttcccg caagtattct tctgatattg atagcatacc tcctgtccca ctcacggggt
     2941 catataatgt tcttacaata ccctctttgg tcaggatttc tctatcattc acgaaaagca
     3001 gattaaccat cagtcggatt acttctctcg gtgtaaagtg ttctcctgct gtttcattgg
     3061 ataattcaga aaatcttctt atgagttctt caaagatgta tcccatctcc aaattgctta
     3121 ctttatcagg atgcaggtca atttccccaa cacgcttaac aatcataaaa agcaaattta
     3181 catcatccaa tcttgcaata tgttcggtga agttaaaata ttcaataatt tccctggcat
     3241 ttgaagagaa accgttaatg taattgttga gattggctgc aatattattt gggtcagctt
     3301 ttagtttct
//
LOCUS       DATGBN010000195         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_325470, whole genome shotgun sequence.
ACCESSION   DATGBN010000195 DATGBN010000000
VERSION     DATGBN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325470"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..362
                     /locus_tag="VKL21_04655"
     CDS             147..362
                     /locus_tag="VKL21_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HMB45093.1"
                     /translation="MFGQGDYTCTVADFTGTMKGPMKGPGGKIIPPTNKKFRVEFCTV
                     AHWKNGEIIEEKLFYDLVGLMRQIGLG"
     gene            376..960
                     /locus_tag="VKL21_04660"
     CDS             376..960
                     /locus_tag="VKL21_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMB45094.1"
                     /translation="MNKNKITTRIYNGLLFPIAGEYAIDPVHSFTCFNAQHLMVGQIW
                     GCFDSLKGKIIISEDPLLSSLDITIDTASLSTHNKKRDEDLSGAKYLDVKNFPTMTYH
                     STGAKAEPGGHLTVEGKLTIRNVTRPVSLDVVFKGIVDDPWGNMRIAFIGNAKISRKD
                     FGLMTDLMRETGGLLVGKDIIINIAIEVLPFSNA"
     gene            1000..>1556
                     /locus_tag="VKL21_04665"
     CDS             1000..>1556
                     /locus_tag="VKL21_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMB45095.1"
                     /translation="MTKEPQTEKPTRPETPEKTSSAPSTENLAARHDRARALLERGAQ
                     AAKHALTLDLLEKVHDPVGEYPYRGWETLHREQWKYDSFGRTTHSINCTGSCTWKVYV
                     RNNIAFKEEQYADYPDINSTLPTYNPRGCQKGANYKEYVYGPQRVKYPLIRTGERGEG
                     KWRKASWEEALNYIAEKIVSAISDHG"
BASE COUNT          473 a          327 c          360 g          396 t
ORIGIN      
        1 tgaagtctat tttttcaata ttcaggcttt taatctcatc agggattgat gtcacaataa
       61 atatataatt atgtaatagc acttaatttt tttggtcggt ttaattatat tttagattcc
      121 cacttgatat tgagcaaata cagattatgt ttggtcaggg ggactacacc tgtactgtag
      181 ctgattttac aggcacaatg aaaggaccca tgaaggggcc tggaggcaaa ataattccac
      241 ccacgaataa aaaattccgt gtagagtttt gcacggtcgc ccactggaag aatggggaaa
      301 taattgagga gaagctcttc tatgatctgg tgggattgat gaggcagatt ggcctgggct
      361 gaacgagaga tccatatgaa caaaaataaa ataacaacaa ggatttataa tggattactt
      421 tttccgatag ctggggagta tgctattgac ccggttcaca gcttcacctg ctttaatgcc
      481 cagcatctga tggtcgggca gatatgggga tgctttgatt cgctcaaagg caagatcatt
      541 atatcagaag atccattact atcatcactt gatatcacta ttgataccgc aagtcttagc
      601 acccacaaca agaaacgcga tgaagatctc agtggtgcaa aatacctcga tgtgaaaaat
      661 tttccaacaa tgacctacca tagcaccggt gctaaagccg aacccggagg ccatttgaca
      721 gttgaaggta aactcaccat tcgcaatgtc acaaggcccg tatcccttga tgtggttttt
      781 aagggcatcg ttgacgatcc ctggggaaac atgcgaattg ctttcatcgg gaatgcaaag
      841 atcagtcgta aagactttgg cctgatgacc gatttgatgc gggaaaccgg tggattgtta
      901 gttggaaaag atattattat aaacattgcc attgaagtcc tgcccttttc gaacgcatga
      961 tgtccggata catatttgat catattgtca cataaagtta tgacaaaaga accccaaact
     1021 gagaaaccga cccgccccga aacaccagaa aagacatcat cagcgccatc gacagaaaac
     1081 ctggctgcaa ggcatgaccg ggcacgcgcg ctccttgaac gtggcgcaca ggcggcaaag
     1141 catgctctga cgcttgacct tctggaaaag gtacatgacc ctgttggtga atacccctac
     1201 aggggatggg aaacgctgca ccgtgagcag tggaaatatg attcttttgg aaggactaca
     1261 cattcgatca attgcacagg ttcctgcaca tggaaggtct atgtcaggaa caacattgca
     1321 ttcaaggagg agcaatatgc tgattatcct gatatcaact caacgcttcc cacctacaac
     1381 ccgagaggat gccagaaagg cgcaaattat aaagaatatg tttatggccc gcagcgtgtg
     1441 aagtatcccc ttattcgaac cggtgagcgt ggtgaaggaa aatggcgaaa ggcctcatgg
     1501 gaagaagctc ttaattatat tgcggaaaag atcgtttctg ccatatccga tcacgg
//
LOCUS       DATGBN010000196         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_209000, whole genome shotgun sequence.
ACCESSION   DATGBN010000196 DATGBN010000000
VERSION     DATGBN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209000"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..642
                     /locus_tag="VKL21_04670"
     CDS             22..642
                     /locus_tag="VKL21_04670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HMB45096.1"
                     /translation="MIKKCHNHGFFRSDTCACGEGGTLMLNDEQTERLGRFISGALRH
                     FPDDLGLAMNQHGWVGVDVLVDAMMTRYKWSNKEKLFSIIESDEKGRYEIKGTKIRAR
                     YGHSVDVDLDYDENILPELYYGASREEVDILLEKGIRPMKQRYVHLSTSAQKAREVAK
                     IHTEDPVLLIVNAEVAQDEGVTMLFATENIVLAEEIPPEYLSVMQE"
     gene            complement(819..1358)
                     /gene="thpR"
                     /locus_tag="VKL21_04675"
     CDS             complement(819..1358)
                     /gene="thpR"
                     /locus_tag="VKL21_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HMB45097.1"
                     /translation="MIRTFIVVDLPDRFIPEIEKIGSTLNTPGIKLVEPKLVHITLKF
                     LGDIKESDIEPIVLALSQVNCRPFEAMIKGVGVFPKPAYIKVVWLGAEGNFDVLHNEV
                     ERVLSPFKFEKDHHFSPHATLARVKQLRNKASLLENLEKLEHIDLGTMKVGSISLKKS
                     TLRPQGPLYEKLAEIKLKE"
     gene            1455..1637
                     /locus_tag="VKL21_04680"
     CDS             1455..1637
                     /locus_tag="VKL21_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HMB45098.1"
                     /translation="MKRDLMDILCCPMCKGDLVLEVTEENEKEIVKGTLFCGKCTEYY
                     PIDDGIPNMLPPELRE"
     gene            complement(1929..>2251)
                     /locus_tag="VKL21_04685"
     CDS             complement(1929..>2251)
                     /locus_tag="VKL21_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45099.1"
                     /translation="AEQLCGCIAFIKKGEIIANAAPGELKRQMKLGEKIIIDYEGKFD
                     ISSLKDIPLILDIKSGNERVMIVAENIEPILDNVLKKFCDVHIKNIEISQPDLEDVFL
                     QLAN"
BASE COUNT          680 a          431 c          475 g          665 t
ORIGIN      
        1 tgattcctat aaacactaga aatgattaag aaatgtcata atcacgggtt ttttagaagc
       61 gacacatgcg catgcggtga agggggaacg ctcatgctta acgacgaaca gacagaaagg
      121 ctcggacgtt tcatctcagg agcattgcgg catttcccgg atgatctggg gcttgccatg
      181 aaccagcacg gctgggtggg cgttgatgtg cttgtggatg ccatgatgac ccggtataaa
      241 tggtcaaaca aggaaaagct tttttcgatc attgagtccg atgagaaagg cagatacgaa
      301 ataaaaggga ccaagatcag ggcgcgatat ggccattctg tggatgttga tcttgattat
      361 gatgaaaata tacttccgga actatattat ggggcaagca gggaagaagt ggatattttg
      421 cttgagaaag ggataaggcc aatgaagcag aggtacgttc atcttagcac atcagcgcaa
      481 aaagcaagag aagtggcgaa aatccatacg gaagacccgg tcttacttat cgtaaatgcc
      541 gaagtggcac aggatgaagg tgtcactatg ctttttgcta ccgaaaatat tgtacttgca
      601 gaggagattc cgcctgagta cctgagtgtg atgcaggagt aacaattaga attgatgaat
      661 gctttttggc caaataataa aatagtatcc acaatatgtt tgtccggtca gattcccttt
      721 cttaccacct aaaggcgtta atggcattgc gattaaagag cattaaaaac catgagcatc
      781 ctcaatgatt cctcggtttt ttcgaatccg atgtcggatt actctttcag ctttatttcc
      841 gccagcttct catataacgg cccctggggt cttagcgtac ttttcttgag ggaaatggat
      901 ccaactttca tagtacccag gtcgatatgt tccagttttt ccagattctc aagcagtgag
      961 gccttattcc tcagctgctt cacacgggca agggttgcat gaggagagaa atggtggtct
     1021 ttttcgaatt taaatggcga aaggacacgt tcaacttcat tatgcagcac atcaaaatta
     1081 ccttctgcgc caagccagac aaccttgata taagcaggct tcgggaacac acccacgcct
     1141 ttgatcattg cttcaaaagg tctgcaattc acctgtgata aagcaagaac aatgggttca
     1201 atatcacttt cttttatatc tcccaggaat ttaagggtta tatggaccaa ttttggttca
     1261 accagtttta ttcccggcgt attaagggtt gatccgattt tttcaatttc aggaataaac
     1321 ctgtctggca aatcaacaac tatgaatgtc cttatcatat tgatcaatgt gaatactcct
     1381 ttaaccaata aaaattatct gattcgataa cttgattata ataaaggtgt tttagggata
     1441 aaggtgtaat caaaatgaaa agagacctga tggatattct gtgctgcccg atgtgtaaag
     1501 gcgatctggt acttgaagtt acggaagaaa atgaaaaaga gatcgttaaa ggtacgcttt
     1561 tttgcggaaa atgtactgag tattatccga tagacgatgg tattccgaac atgctgcccc
     1621 cggaattaag ggaatagcag ataattatag gaaacagaat taaagaagca aaaacagggc
     1681 ttcaaaattt ttttatgttt ttaacagagt ttcctcaata gtaaaagcct ctcttccaca
     1741 atccgtgcat gatacaatcg caattccttt gatgaaatca gcgtttctta gctgtattaa
     1801 atgcttgcaa ttagggcatt tcttcataac aaattctgat tgaattaaag tctccatcat
     1861 aattatcaat tcttaatgat tattcattga tttaagactt gtcataatga tccgggtatg
     1921 aaagcctatc aattcgcaag ctgcaggaaa acatcttcaa gatcgggctg ggaaatctcg
     1981 atatttttaa tatgaacatc gcagaacttc tttaagacat tatcaagtat tggctcaata
     2041 ttttctgcaa cgatcattac cctttcattt ccggatttga tatcaaggat tagcgggatg
     2101 tccttcaatg aagaaatatc aaacttgcct tcgtaatcga taattatttt ctcccccagt
     2161 ttcatctgtc tcttaagttc accgggcgcg gcattggcga ttatttcacc cttctttata
     2221 aatgcaatgc atccgcatag ctgttcagct t
//
LOCUS       DATGBN010000197         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_480702, whole genome shotgun sequence.
ACCESSION   DATGBN010000197 DATGBN010000000
VERSION     DATGBN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480702"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /gene="larB"
                     /locus_tag="VKL21_04690"
     CDS             <1..872
                     /gene="larB"
                     /locus_tag="VKL21_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HMB45100.1"
                     /translation="YFLYLKLLVLSLLFRKNLSIDKNKVYASVILHMEISDILLKLKN
                     NEISVKDAQLQLRLNNYESVLDIAKVDIHRMKRVGIPEAIIADCKTCEEVASIAHVHL
                     EKEGRVIITRVREENYATLKSLAEETKSKIRWEKRARIIIIGETLTRSGGKIGVISAG
                     TADIPVAEEAKVIAEEMGCAVSVIYDVGVAGIHRLFPELTKLVKAGVDAIVVAAGREG
                     TLPTIVSGLVDVPVIGVPVSTGYGAGGKGEAALLSMLQSCSVLTVVNIDAGFTAGAFA
                     ARIANLAAKNRTL"
     gene            991..1401
                     /locus_tag="VKL21_04695"
     CDS             991..1401
                     /locus_tag="VKL21_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1699 family protein"
                     /protein_id="HMB45101.1"
                     /translation="MKIRIVSSKEEINYLNQNEKIIHLAFRPSNKDIFALVHTCPGVK
                     AIHIPSSYIKTISNSTKMFLQMQDVALLEGDVWGHRKDINEYSEINPEVFERMEELKA
                     DGLSESAVLDRLVRETRLSKDLLKFLSKDKSKKK"
     gene            complement(1413..1709)
                     /gene="tatA"
                     /locus_tag="VKL21_04700"
     CDS             complement(1413..1709)
                     /gene="tatA"
                     /locus_tag="VKL21_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMB45102.1"
                     /translation="MVFGAPEIILIVIIVILLFGANKIPELAKSLGKATGEFKKGKHE
                     IEAELSDIGKPVKEGKSLENTSSKIKKMAQDLGIVTEGKNDEQLLDEIQKKNKK"
     gene            complement(1712..2020)
                     /locus_tag="VKL21_04705"
     CDS             complement(1712..2020)
                     /locus_tag="VKL21_04705"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMB45103.1"
                     /translation="MGLIGIPELILILLVALFLFGPDKIPEIARSLGKAAGEFKKAQT
                     ETEHEIKRLDEPLNEKDTKIHNLAIEMGLDVKNKNSEQLVEEIRSKIRTDGINQSTAA
                     "
BASE COUNT          685 a          403 c          441 g          684 t
ORIGIN      
        1 gttattttct atatttaaaa cttttggttt tatcgcttct ttttagaaaa aacctttcaa
       61 tagataagaa taaagtctat gcaagcgtaa tcctgcacat ggaaatatct gatatattgc
      121 tgaaattgaa aaacaacgaa ataagtgtta aagacgcgca attacagctt cgcctcaaca
      181 actatgaatc ggtattagat attgcaaaag tggacataca tcgcatgaaa agagtgggta
      241 tccctgaagc aattatcgct gactgcaaga catgtgaaga agttgcatct atagcgcatg
      301 tacatctgga aaaagaaggg cgtgtaataa taacacgagt acgagaagag aattatgcaa
      361 cattgaaatc attagccgaa gaaacaaagt ccaaaataag atgggaaaag cgtgcaagga
      421 tcatcataat aggagaaaca ttaacacggt ctggaggaaa aattggggtt atatcagcag
      481 gaacagctga tatccctgtc gcagaagaag caaaagtgat agcagaagag atgggttgtg
      541 ctgtttctgt aatatatgat gtgggagttg cgggtatcca caggctattt ccagaattaa
      601 caaagcttgt aaaagcagga gtggatgcta ttgtggttgc cgccggccgg gaagggacac
      661 ttcctacaat tgtatctggg ttggtggatg ttccagtcat tggagtgccg gtctctacag
      721 gatatggcgc aggcgggaaa ggtgaagctg ctcttttatc catgcttcaa tcatgttctg
      781 ttttgactgt tgtaaatatt gacgcaggtt tcacggccgg ggcatttgct gcaaggatag
      841 ctaacctggc tgcaaaaaat cgcactttgt aacatattct aaatatacat ttgaaaacga
      901 agattattta tatggtataa atgaatatag ttatcgtgtg ttgaattaat ttttattgtg
      961 aaagagaaat taattaagag gtaatcagat atgaaaattc gaattgtaag ttccaaggaa
     1021 gagatcaatt atcttaacca gaatgaaaag atcattcatc ttgcttttag accatcaaat
     1081 aaggatattt tcgcattagt gcatacatgt cctggcgtga aagcaataca tattccgagt
     1141 tcctatataa agacaatctc aaactctaca aaaatgttcc tgcaaatgca ggatgtagcc
     1201 ctgcttgaag gagatgtttg gggacacagg aaagatatca atgagtattc tgaaataaat
     1261 ccagaggttt tcgagaggat ggaagagctt aaagctgatg gattatcgga gtctgcagtt
     1321 ctggacaggc ttgtaagaga gacacgcctg agcaaagact tattgaaatt cctttcaaaa
     1381 gacaaatcaa aaaagaaata aattttattt tcttatttct tatttttttt ctggatctca
     1441 tccagcaatt gctcatcgtt tttgccctca gtcacaatac cgagatcctg agccatcttt
     1501 tttattttgg aagatgtatt ttccagggat ttaccttctt tgacaggttt cccaatatca
     1561 cttaattccg cctctatttc atgttttccc ttcttaaatt cccctgtggc ttttcccagt
     1621 gacttggcaa gttcagggat cttgttcgct ccaaaaagca ggatgacaat tattacaatt
     1681 aatattattt ctggagcacc aaataccata attacgccgc cgtactttga ttaataccat
     1741 ctgttcttat ttttgaacga atctcttcta caagttgttc actgttcttg tttttcacat
     1801 ctaaccccat ttcaatcgct agattgtgga tcttagtatc tttctcattc aaaggttcgt
     1861 ccagtctttt gatctcatgc tcggtttcag tttgagcttt cttgaattca ccggcggctt
     1921 ttcccagcga ccgtgcaatt tccgggatct tatccggccc gaataagaat aaagcaacca
     1981 acaatatcaa tattaattca ggaattccta ttaggcccat atgtataatg ttattatttc
     2041 attacttaaa tctttgcttt cttaactttg caccagctat ctgccctgct atatctgata
     2101 agataagcaa aggaatgacc gtatagttca gccctgaaaa tatcctcatc aagaaaacga
     2161 atggcacagg gatgtgtatt gccattatcc atttcctgga gaacctttgc gcg
//
LOCUS       DATGBN010000198         5396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_14636, whole genome shotgun sequence.
ACCESSION   DATGBN010000198 DATGBN010000000
VERSION     DATGBN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14636"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2419)
                     /locus_tag="VKL21_04710"
     CDS             complement(<1..2419)
                     /locus_tag="VKL21_04710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HMB45104.1"
                     /translation="MVIATPKRIKQIRFGLISPKEFRKMSVTRIITADTYDDDGFPIE
                     MGLMDTRLGVIDPGLRCKTCGGRAGECPGHFGHIELVAPVMHVGFAKLIRKILRATCR
                     NCGALLLDPTQKKETVEKIRVLKKMGQSIEDVVNDVFKDARKAGKCQYCNEEQREIKF
                     EKPTTFIEDGHKLMPTDIRSRLEKMTDEDIEVIGMDSKNARPEWIILTVLAVPPVTMR
                     PSITLESGQRSEDDLTHKLVDIIRINQRFQENREAGAPQLIIEDLWELLQYHITTFID
                     NAVSGVPPARHRSGRPLKTLSQRLKGKEGRFRGSLSGKRVNFSARTVISPDPNLGMHE
                     VGVPLAIAKELTITLYVTPRNINEVKEYVRRGPDNHPGANYIVRADGRRLRITDKNSN
                     ELGDLVELGWKVDRQMKDGDIILFNRQPSLHRMSIMAHEVKVLPHKTFRLNPAVCPPY
                     NADFDGDEMNMHVPQTEDARAEAKILMHVRENIISPRFGGPIIGGIHDHISGLFLLTN
                     SKDKINKDDALELLAKSEIWELPEPAGKENGKPYWNGKQIFSQILPKGLNLEFKAEIC
                     EQCDTCKGVDCEHDAYVVIKDGVLERGSIDEKAVGAFKSVILDKLIKEFNTALACKFI
                     DDATRLSIRGIMRSGFSFGINDEYVPVDAQNQINETLNNATDKVEKLILAYEAGELEQ
                     LPGRTIRETLEMEIMKELSRARDATGDIAGRHLGMDNSAVIMAKSGARGSMLNLTQMA
                     ACVGQQAVRGERIQRGYAGRTLSHFDKGDLGAQAHGFVKASYKGGLSPTEYFFHTIGG
                     REGLVDTA"
     gene            complement(2429..4243)
                     /gene="rpoB"
                     /locus_tag="VKL21_04715"
     CDS             complement(2429..4243)
                     /gene="rpoB"
                     /locus_tag="VKL21_04715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HMB45105.1"
                     /translation="MSKARVFLNGELIGTIANPKELVHNLRKKRRMGEIKKQVNVIFY
                     EDTNEIIINSDQGRARRPAIIVENGKPLVTPEHIERLKKKEITFDSLTDAGLIEYLDA
                     EEEENAFIAIDEKNLNSKHTHLEMDPSLILGICTGMVPFPEHNASPRNTMGAGMVKQC
                     LGMSTPNMKLRPDTRAHVLHYPQKALTSTQTAEAIGFDTRPAGQNFVVAVLSYEGYNI
                     EDALVINKGAIERGLGRSHFFRTNEEEKRKYPGGQEDKFEIPDTEVRGARTAEVYNHL
                     DGDGLVNPEIPVGPNDVLIGKTSPPRFLEEPTELGLSPVQRRESSVTMRSNEKGIVDT
                     VILTESENGSRLAKVRTRDYRIPEIGDKFASRHGQKGVIGLIVPYEDMPFTENGLVPD
                     LIINPHAIPSRMTVGHVLEMIGGKVGGLEGRRIDGTAFSGESEEDLTSSLAKFGFSNT
                     GKEVFYDGITGNKIQADIFVGVILYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVLIGHGAAMALKERLLDESDRVMEYVCSHCGMIATLDKRRNTTICHD
                     CGAETDIHPVEMSYAFKLLLDEIISLGVAPRLELEDAV"
     gene            complement(4236..>5396)
                     /locus_tag="VKL21_04720"
     CDS             complement(4236..>5396)
                     /locus_tag="VKL21_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HMB45106.1"
                     /translation="FFIVNGTERVITTLEDLAPNKILVEMEERYGENIEVAKVFSQRR
                     GYRALVVVERNKKSILEVSFPSISGRINFITLMRALGIETDQDIVNAVSDDPEIIKFM
                     LENLEEAEAANKVEAMEKIGLRVASGQSRDYQVKRANYVIDRYLLPHLGNDEIHRIIK
                     AQFIGRMAQACFELALGKRASDDKDHYANKRLKLSGELIEDLFRVSFNRLTRDIKYQL
                     ERASMRNRDLNVTTIVRSDVLTERLVHALATGNWVGGRTGVSQLLDRTDYIATLSHLR
                     RVISPLSRAQPHFEARDLHPTQWGRVCPSETPEGPNCGLVKNFAQLVEISTAAGNEAQ
                     LKKMLLEMGTAPIIPQLLGMEEVPTTYATELEALEEAEETKIMPEEEAESFE"
BASE COUNT         1299 a         1358 c         1044 g         1695 t
ORIGIN      
        1 cagctgtatc cacaagacct tctcgccctc ctatggtgtg gaagaaatac tccgtgggcg
       61 ataatccacc cttataactt gctttcacaa atccgtgggc ttgcgctcca agatcacctt
      121 tatcaaagtg ggataaagtc cgtcctgcat accctctctg gattctctca cctcgaacgg
      181 cctgctgtcc tacacatgcc gccatctggg tcaggttaag catcgaacct cttgctcctg
      241 actttgccat tatcactgcc gaattatcca ttccaagatg gcgtcctgca atgtcgcctg
      301 ttgcatctct tgcccggctt aattccttca ttatctccat ttcaagggtt tccctgatgg
      361 ttcgcccggg cagctgttca agctcgcctg cttcgtaagc aagtatcaat ttttccactt
      421 tatcagtagc attgttcaag gtctcattta tctggttctg agcatcaacg ggaacgtatt
      481 cgtcattgat cccgaaactg aagccacttc gcatgatccc ccttatggaa agacgagtgg
      541 catcatcgat aaatttacac gctaaagcgg tattgaattc ttttatcagt ttgtcaagta
      601 tcacactctt gaaagcgcca actgcttttt catcaattga ccccctctca aggaccccgt
      661 ctttgataac tacatatgca tcatgttcgc aatcaacgcc tttacatgta tcgcattgtt
      721 cacatatttc cgccttaaat tccaggttaa ggccttttgg aagtatctgg ctgaatatct
      781 gttttccatt ccagtatggt tttccgttct cttttcctgc cggttccggc aattcccata
      841 tctctgattt tgcgaggagt tccagtgcat catctttatt aattttatcc ttactgtttg
      901 taaggaggaa aagaccggaa atgtggtcat ggataccgcc aatgatagga cctccaaatc
      961 tgggggatat gatattctcc cgaacatgca tcaatatttt tgcttccgca cgtgcatctt
     1021 cggtttgtgg tacatgcatg ttcatttcat caccgtcaaa atcagcgtta tatggcgggc
     1081 aaactgccgg gtttagcctg aaagtcttat gcggcagcac ttttacttca tgcgccatta
     1141 tactcatcct gtgaagcgat ggctgcctgt tgaataaaat gatatcgcca tctttcattt
     1201 gcctgtcaac tttccatccc aattcaacaa ggtctccaag ttcattggaa ttcttatcag
     1261 tgatccttaa ccttcttcca tctgccctga cgatataatt ggcccccggg tggttatccg
     1321 gaccgcgccg aacgtattcc tttacttcat taatattcct gggtgttaca tacaatgtta
     1381 tagtgagttc cttggctatc gccaggggaa ctcccacttc atgcatacca agattagggt
     1441 caggtgagat cacagttctt gcagagaaat taacacgttt cccggataaa cttcccctga
     1501 accgtccttc tttacctttt aatctctggg atagggtctt gagcggcctt ccggacctgt
     1561 ggcgggccgg aggtactcct gaaactgcat tatcaataaa tgtagttata tggtactgca
     1621 ataattccca tagatcttca ataattaatt gtggcgcccc agcttcacgg ttttcctgga
     1681 acctctggtt aatgcggatt atatccacaa gtttgtgagt aaggtcatct tcgctccttt
     1741 gacccgattc aagagtaatc gatggcctca tcgttacagg aggaacagca aggactgtaa
     1801 ggattatcca ttccggacgc gcattcttag aatccatacc gatcacttca atatcctcgt
     1861 cagtcatttt ttcaagacgt gaacgaatgt cagtaggcat cagtttatga ccgtcttcta
     1921 tgaatgttgt gggtttttca aatttgatct ctctttgctc ttcattgcaa tactggcact
     1981 ttccagcctt tcgtgcgtcc ttaaaaacgt cattcacaac atcttcaatg gattgtccca
     2041 tttttttaag aactcgtatc ttttcaacag tctctttttt ctgggtggga tcaagaagca
     2101 aagctccaca atttctgcag gttgctctta agatcttgcg tatcagcttt gcaaaaccta
     2161 catgcattac aggcgcaaca agttcaatat gcccgaaatg acccgggcac tcgcctgctc
     2221 ttccgccgca cgtcttacat ctcagaccgg ggtcaatgac cccaagtctt gtatccatta
     2281 atcccatttc aataggaaaa ccgtcatcat catatgtatc ggcagttatg attctagtga
     2341 cactcatttt cctgaattct tttggtgaaa taagtccgaa tctaatctgt tttattcttt
     2401 ttggggttgc aattaccata atttacacct atacagcatc ttccaattcc agcctcggtg
     2461 ctacacccag tgaaattatt tcatcgagga gcaatttaaa ggcatagctc atctcgacag
     2521 ggtgtatatc cgtttccgcg ccacagtcat ggcaaattgt agtattcctc cgtttatcaa
     2581 gtgtggctat catgccacag tgagaacaaa catattccat gacccgatca gattcgtcaa
     2641 gcaatctttc cttgagggcc attgcagcgc catgtccgat aagaacatct cgttccattt
     2701 ctccaaacct caagcctcct tctcttgccc gaccttcagt tggctgtctg gtcaatacct
     2761 gtacaggtcc acgtgacctg gcatgcattt ttgaagatac catatggtac agtttctggt
     2821 acagaatgac tccaacaaag atatccgcct gtatcttatt gccagttatg ccatcataaa
     2881 aaacttcttt tccagtgttt gaaaatccaa atttcgctag tgaggatgtc aaatcctctt
     2941 cggattcacc tgagaaagct gtaccgtcga tcctgcgtcc ttcaagcccg ccaactttac
     3001 ctcctatcat ctcaagaacg tgacctacag tcattcttga aggtatagca tgtggattaa
     3061 taattagatc aggaaccagg ccattctcgg taaaaggcat atcctcataa ggcactataa
     3121 gaccgattac acctttctgg ccatgccgtg aagcgaattt atccccaatc tcgggtatcc
     3181 tgtaatcacg cgtccttact tttgcaagcc ttgaaccgtt ctcggattcg gtaagtatta
     3241 ctgtatctac aatacccttc tcatttgaac gcatcgtgac agaactctct ctgcgctgca
     3301 cggggctcaa tcccaattct gtgggctctt ccaggaacct tggggggctg gtctttccga
     3361 tcaggacatc gtttggaccg actggtattt cgggattaac aagaccatcg ccatccaggt
     3421 ggttataaac ttccgcagtt ctggcgcccc ttacttccgt atcggggatc tcaaatttgt
     3481 cttcctggcc tcccgggtat tttctttttt cttcttcgtt tgtcctgaag aaatgacttc
     3541 ttccaagacc gcgttcgatc gcacctttat taatgacaag ggcatcttca atattatacc
     3601 cttcataaga taatacagca acaacgaaat tctgtcctgc cggacgggta tcaaaaccga
     3661 tcgcttctgc ggtctgggtt gaagtgagtg ctttctgggg ataatgcaat acatgggctc
     3721 ttgtatccgg tcttaatttc atattcggtg ttgacatgcc aagacactgt ttaaccatgc
     3781 ctgcacccat tgtattcctt ggtgatgcat tgtgttccgg gaatggtacc ataccagtac
     3841 aaattcccag gatcaaagac ggatccatct caagatgagt atgttttgaa ttcaaatttt
     3901 tttcatctat tgcaatgaac gcattttctt cttcttcagc atccagatac tcgatcaaac
     3961 ctgcatctgt aagactatca aaagtgatct ccttcttttt caggcgttca atatgttcgg
     4021 gtgttaccag tggtttgcca ttttcaacaa ttattgccgg acgtcttgct cttccctgat
     4081 ccgagtttat aataatctca tttgtatcct catagaatat tacatttacc tgttttttga
     4141 tctcacccat tcttcgtttt tttctcaaat tatggacaag ttcttttgga ttggcaatgg
     4201 tgccaataag ttcaccgttt aaaaaaactc ttgccttact caaatgattc agcctcctct
     4261 tccggcatta tttttgtttc ttcagcttct tcaagcgctt ccaattccgt ggcatatgtt
     4321 gttgggactt cttccattcc aagaagctgg ggtatgatcg gcgccgtacc catttccagg
     4381 agcattttct taagttgcgc ttcattaccg gcagccgttg atatctcaac cagctgcgca
     4441 aagtttttca ccagtccgca attggggcct tcaggagttt ctgatgggca gactcttccc
     4501 cactgggtgg gatgcaagtc ccttgcctca aaatgcggtt gtgctcttga caaaggtgaa
     4561 atgacccttc tcaaatggga cagtgttgca atataatctg tacggtcaag taattgcgac
     4621 acacctgtcc tgcctcctac ccagttacct gtagccaggg catgtacaag acgttcagta
     4681 agcacatcgg atcggacaat agtggtcaca ttcaggtccc tgttcctcat gctggctctt
     4741 tccagctggt atttgatatc acgagtgagc ctgttgaaag ataccctgaa caggtcttcg
     4801 atcaattcgc cggacaattt taacctctta ttcgcatagt ggtctttatc atcactggct
     4861 ctttttccca gcgccagttc aaaacatgcc tgagccatgc ggccaatgaa ctgtgcttta
     4921 atgatcctgt gaatttcatc gttcccaaga tgtggcaata aatacctgtc aataacataa
     4981 ttcgctcttt tgacctgata atccctggat tgtccggaag ccactctcaa cccgattttt
     5041 tccattgctt cgaccttgtt ggcagcctcg gcttcttcaa ggttttcaag catgaatttg
     5101 attatctcag gatcatcgga aacagcattg acaatgtcct gatcagtctc aatacccaga
     5161 gccctcataa gagtgataaa atttatcctg cccgatatgg atggaaatga aacttcaagg
     5221 atcgactttt tgtttctctc gactacgacg agggcgcggt agcccctgcg ttgtgaaaat
     5281 actttggcaa cttcgatatt ttcaccatat cgttcttcca tttccacgag gatcttattc
     5341 ggcgcaaggt cttcaagcgt agttataacg cgttcggtac cgttaactat aaaaaa
//
LOCUS       DATGBN010000199         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_325777, whole genome shotgun sequence.
ACCESSION   DATGBN010000199 DATGBN010000000
VERSION     DATGBN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325777"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VKL21_04725"
     CDS             complement(<1..298)
                     /locus_tag="VKL21_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HMB45107.1"
                     /translation="MKSKKKECEALIVSMNLIADHIKKVASKLDTDSVTLLVNGIIDS
                     KIIFLMGAGRSGLAAKAFAMRLMHLGFDVYVVGETTTPAVQPDDLVIAVSGSGET"
     gene            complement(295..660)
                     /locus_tag="VKL21_04730"
     CDS             complement(295..660)
                     /locus_tag="VKL21_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45108.1"
                     /translation="MIHDVIFGISSIDSSVTLEQEILCEFKNISKYENVGYVLTSYAK
                     TRGGYRVIFNIPFHKKIAMAHFVRSIVEQLREKDIRKTLLWNGSPEKINFIYNKLKNL
                     EGWEIKRIEYKDKEEKVLL"
     gene            complement(847..1716)
                     /gene="mtnP"
                     /locus_tag="VKL21_04735"
     CDS             complement(847..1716)
                     /gene="mtnP"
                     /locus_tag="VKL21_04735"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HMB45109.1"
                     /translation="MKGRRNMQVDIAVIGGTGVYDTAMLDNVQEVEIDTPFGKPSDAI
                     TIGSFGEINVAFLPRHGKGHRISPSELNSRANILALKKLGVKRIISASAVGSLKLEHA
                     PLDIVIPDQIYDRTRMRDSTFFEDGIVAHIGFADPFCPEMSSMIAGITRKLGYSVHEK
                     GTYVCMEGPQFSTRAESRVYQGLGFDIIGMTALPEAKLAREAQICYSMIATVTDYDVW
                     HETDVNIETVISNAMKNEDAVRKIIKEIIPRISFERNCVCSNALAGAIVTAPDKIPVE
                     TKRKLADLIGKYI"
     gene            complement(1703..2407)
                     /locus_tag="VKL21_04740"
     CDS             complement(1703..2407)
                     /locus_tag="VKL21_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HMB45110.1"
                     /translation="MVLPDKFKCVITNWDYIYDLCRHVANDVKRSGYEPDTIIALARG
                     GWFAGRVLCDFLGLNDLTSLKIEHYVGTANAGSGPSIRYPLADNAVAGKRVLIVDDIT
                     DTGKSIAHSKEYVSKQNPKEVRTAVLQYLYTSEIKPDYCGEVVSEWGWIVFPWNFIED
                     MIDIISRLMVKEKQNEWNIEKIRAGLLKYHNIDPISFEIAQPGRMEEILDEMVRRRLI
                     KSKNNSGKKTWTYEGT"
BASE COUNT          695 a          637 c          465 g          864 t
ORIGIN      
        1 gagtttcccc ggaacctgat actgcaatta caagatcgtc cggctgaact gccggggtgg
       61 ttgtttcacc tacgacataa acatcaaaac caaggtgcat aagtctcatc gcaaaagcct
      121 ttgccgcaag cccggatctt ccggctccca tcaggaatat tattttagaa tctattatgc
      181 cattaacaag caatgtcact gagtctgtat caagttttga tgccaccttt tttatatgat
      241 cagcgataag gttcatcgac acaattaatg cttcacattc tttcttcttg gatttcatag
      301 taatactttt tcctctttgt ctttatattc gattcttttt atctcccatc cctcaaggtt
      361 tttcaattta ttgtaaataa agttgatctt ctcagggctt ccattccaga gaagcgtttt
      421 ccttatgtcc ttttcccgga gttgttctac gatcgatcta acgaaatgtg ccattgctat
      481 ctttttgtgg aaaggtatat taaaaataac cctataacct cctctggttt ttgcgtatga
      541 tgtcaaaaca tatcccacat tctcatattt gctaatgttc ttaaattcgc ataaaatttc
      601 ctgttcaaga gtaacggatg aatcaatact ggatattcca aaaatcacat cgtgaatcaa
      661 cgattcaccc gcccgcctgc caaaccataa tattatttag cctgtggaaa gggaaacgct
      721 gatatcatcg cgcccgagtt caacaatatt tacttcctta ctatctttta gaaaaatgga
      781 ttttccggaa ccctcctttt tagcgctcat ttatctacca tgatataaat attacttatt
      841 aaataattat atatatttac cgatcaaatc tgccaatttc cgctttgtct cgacaggtat
      901 cttatcaggt gcagtgacga tcgcccctgc caatgcattt gagcatacgc aatttctctc
      961 aaatgaaatc cttggaatga tttctttaat aattttacgt actgcatcct catttttcat
     1021 tgcattagaa ataaccgttt caatattgac atcagtttca tgccagacat cataatcagt
     1081 aactgttgcg atcatgctgt agcagatctg ggcttcccgg gcaagttttg cttcaggaag
     1141 agcggtcatc ccgatgatat caaacccgag cccctggtaa actcttgatt ctgcgcgtgt
     1201 ggaaaattgc ggcccttcca tacagacata cgttcctttt tcatgaacag aataccccag
     1261 tttccttgtt atccctgcga tcattgaaga catctcagga cagaaaggat cggcaaaacc
     1321 gatatgggca actattccgt cttcaaagaa agtgctatct ctcattctag tcctgtcata
     1381 tatctggtca ggtattacga tgtcaagggg tgcatgttca agtttaaggc ttcccaccgc
     1441 tgatgccgag ataatccttt tcacgccgag ttttttgagc gccaggatat ttgccctgga
     1501 attcaattca ctcggtgaga tcctgtgccc tttcccgtgc cttgggagaa atgcaacgtt
     1561 aatttcacca aaagacccaa ttgtgatagc gtctgatggt tttccgaaag gcgtatctat
     1621 ttctacttcc tgtacattat ccagcattgc cgtatcgtaa acgccggtcc ctccaattac
     1681 tgcaatatct acctgcatat ttctacgtcc cttcataagt ccatgttttt tttcctgaat
     1741 tgttcttcga cttgatcagt cttcttctta ccatctcgtc aagtatctct tccatacggc
     1801 caggctgtgc gatctcaaag cttatcgggt cgatgttgtg atactttaat aatcctgccc
     1861 tgatcttttc aatattccat tcattttgct tttccttgac cataagtctg gaaatgatat
     1921 ctatcatgtc ttcgataaaa ttccagggga atactatcca gccccattcc gaaacgactt
     1981 caccgcaata atcgggtttt atctccgatg tgtacaagta ttggagcact gcagtcctta
     2041 cctctttggg attctgtttg ctcacgtatt cttttgagtg cgcgatactt ttacctgtgt
     2101 cggtaatatc atcaacgata aggactcttt ttcccgccac tgcattatct gcaagcggat
     2161 acctgatgct gggcccactt cccgcgtttg cggtacccac atagtgctcg atcttaaggc
     2221 ttgtcaggtc attgagcccc agaaaatcgc aaagcaccct tccggcgaac catccgccgc
     2281 gcgccagcgc tattatcgta tcgggttcat accccgatct tttcacatca ttcgctacat
     2341 gcctgcacag gtcgtaaata tagtcccagt tagttatgac acatttgaat ttatccggaa
     2401 gtaccattat gtcgccaccg caaacgaaac ggtattacag gttaatatat tttttggaaa
     2461 atattcgaac tctacagaaa ataatcttaa aaatccggaa cataccctga tatttattaa
     2521 aataagcttt ctattagctt ggcgagcaga tgtttttcgt aggcttcttt actacttgcg
     2581 aattgctgcc acaacccgtc aacagcagat ggtaatatgc atctcttgaa tacagtgagt
     2641 gtcacataat tgatatccat a
//
LOCUS       DATGBN010000200         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_364969, whole genome shotgun sequence.
ACCESSION   DATGBN010000200 DATGBN010000000
VERSION     DATGBN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364969"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2647)
                     /gene="clpB"
                     /locus_tag="VKL21_04745"
     CDS             complement(53..2647)
                     /gene="clpB"
                     /locus_tag="VKL21_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HMB45111.1"
                     /translation="MSIRFDRFTIKAQEAFQEAQTIALDRGQQQLEVEHLLLALINQK
                     EGLTLQLLQKLGANTGGIISKLEEEIDKLPHVEGAGAGQIYMSTRLNNAAEVASIEMK
                     NLRDEYLSSEHLLLAIADEKNGISARILKENGVSKDAIMKSLKEVRGSSRVTDQNPED
                     KYEALKKYGRDLTELAAIGKLDPVIGRDNEIRRVLQVLSRRTKNNPVLIGEPGVGKTA
                     IAEGLALRIFAGDVPETIKDKKVVALDMGALVAGAKFRGEFEERLKAVIKEVQDSQGA
                     VILFIDELHTLVGAGAAEGAMDASNLLKPALARGELHVIGATTLDEYRKHIEKDAALE
                     RRFQPVLVDEPSVDDTISILRGLKEKYEVHHGVRIQDAALVRAAILSHRYITDRFLPD
                     KAIDLIDEAASKLRIEIDSMPSELDDIERKIRQLEIEKQAVKKEKDEYSKERLEKIDK
                     ELSDLKEKGSQMKAHWQLEKEAIGKIRKTKGDIEKTRMDSEKAEVEGNLERASELRYG
                     TLINLQKQLDGENKKLVKLQKDQKMLKEEVDEEDIAEVVSKWTHIPVSKMLEGEMQKL
                     VHMEERLRQRVIGQEEAVVAVANAVRRGRAGISDPKRPIGSFMFLGPTGVGKTELGRA
                     LAEFMFDDEAAMIRLDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAIRRRPYAVIL
                     LDEIEKAHPDVFNVLLQILDDGRLTDGHGRTVDFKNTVIIMTSNIGSQLIFELQEKET
                     QMRKKVMDVVRSHFKPEFLNRLDEIIIFHSLKLPEIVKIVDIQFNILKKRLTERKIEI
                     ILTESARNLIARIGYDPSYGARPLKRTIQHKILDPLAMKILDKEFGQGDTIEVDADGE
                     EIMFRKVD"
     gene            2786..3514
                     /locus_tag="VKL21_04750"
     CDS             2786..3514
                     /locus_tag="VKL21_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45112.1"
                     /translation="MPKTNRTAPLYDMLFSEGKFNGTLLIGPDPLGATFDYGVFLEVR
                     RRLRLIGLTLTESRRGFEEYEKAFNFNNRIIRARIRLFLGRNFGGDLQEIWRQTLTNE
                     DLIYLKTHAGYGKHLSLSDDVSYFADAMKEGFDRPGRKPYQLYYLDCCKSEMYYKDVF
                     RKYVGGVDLILHKWFCDYRIIGPVVVLIRELMAGSDFETIILKMNEEYGIPHFDVEDD
                     PADMRLDRKMVTYSLGDSVRRNST"
     gene            3604..3879
                     /locus_tag="VKL21_04755"
     CDS             3604..3879
                     /locus_tag="VKL21_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45113.1"
                     /translation="MYKLLKNDGTFYLSDVVFSFPIEEYDYKIDNWIDTMKEKVGKNF
                     AEEAQMHIKEEYSTFDWILEEMLPKSGFRFDIRHKDDFSAVYYCKKK"
     gene            complement(3901..>4264)
                     /locus_tag="VKL21_04760"
     CDS             complement(3901..>4264)
                     /locus_tag="VKL21_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45114.1"
                     /translation="LQIQNMVQNHHLAKSIHDASWSNLINLTKYKAEYAGKVVELVNP
                     KGTSQTCICGCRVPKDLSVRIHNCPLCGLVLGRDHMSAILIKNKSISTVGTTGLQARP
                     SNLNREPMKREARLFKGE"
BASE COUNT         1028 a         1010 c          942 g         1284 t
ORIGIN      
        1 acatcagtcc aatgaatgtc atttacccgg aaaaaaatca aactgtctcc ttctaatcaa
       61 ctttcctgaa catgatctct tcaccatccg catccacttc tatcgtatca ccctggccga
      121 attccttatc cagtatcttc attgcaagag ggtcaaggat cttatgctgg atagtcctct
      181 ttaatggccg cgcgccgtat gaggggtcat aacctattct tgcaatgagg tttcttgcag
      241 attcggtaag aataatttcg atcttccttt ccgtcaggcg ttttttcagg atattgaact
      301 ggatatcaac gatttttact atttccggaa gtttaagcga gtggaaaatt atgatctcat
      361 caagccggtt caggaattca ggtttgaagt ggctcctgac aacatccatc actttctttc
      421 tcatttgcgt ttccttttcc tggagctcaa aaatcaactg gctgcctata ttggaagtca
      481 taattattac tgtatttttg aaatctacgg tacgcccatg gccgtccgtg agccgcccgt
      541 catcaagtat ctgaagcagc acgttgaaga catcggggtg tgctttttct atttcatcca
      601 gtaaaattac cgcatatggc cggcgcctga tggcttctgt aagctgtccg ccttcttcat
      661 agcccacata tcccggcggc gcgccgatga gccttgcaac cgtgtgcttt tccatatact
      721 cgctcatatc aagccgtatc attgcggcct cgtcatcgaa catgaattca gcaagcgccc
      781 ttccaagttc agtctttccc acgcctgtgg ggccaaggaa catgaaggaa ccgataggac
      841 gctttggatc tgaaattccg gcacgtccac gcctcacggc attggcaact gcgacaacag
      901 cttcctcctg cccgatcaca cgctggcgga ggcgctcttc catgtgcaca agtttttgca
      961 tctccccttc aagcatttta ctgaccggga tgtgcgtcca tttcgagacc acttctgcga
     1021 tatcttcttc atcaacctct tcttttagca tcttctggtc tttctggagt ttgacaagtt
     1081 tcttattttc cccatcaagt tgtttttgca gatttatcaa ggtcccgtaa cggagttcgg
     1141 acgcccgttc aaggttgcct tcaacttccg ctttctcaga atccatgcgg gtcttctcga
     1201 tgtctccctt ggtctttcgt atcttcccga tggcttcctt ttcaagctgc cagtgagctt
     1261 tcatctgact gcctttttcc ttcaggtctg aaagttcttt atctattttt tcaagccgtt
     1321 cctttgaata ttcgtccttt tcttttttga ccgcctgctt ttctatctca agctgccgta
     1381 tcttcctctc gatatcatca agctcggaag gcatactatc gatctctatc cgaagctttg
     1441 aagccgcttc atcaataagg tcaatagcct tatcgggaag gaacctgtct gttatatatc
     1501 tatgcgaaag gatcgctgcc ctcacaagcg cagcatcctg tatcctcacg ccgtggtgca
     1561 cttcatattt ctccttcagg ccgcgaagga tggatattgt atcatccacc gatggctcat
     1621 ccactagcac cggctggaaa cgccgctcaa gagccgcatc cttttctatg tgcttgcgat
     1681 attcatccag cgtggtagcg ccgatgacat gtagctcccc gcgcgcaagc gccggcttga
     1741 gaagatttga ggcatccatc gccccttccg cagccccggc gccgacgagt gtgtgcagtt
     1801 cgtcgataaa taggattacc gcaccctggg aatcctgcac ttccttgatc acggctttca
     1861 gtctttcttc aaattctccc ctgaatttcg cacccgccac aagcgcgccc atatcaagcg
     1921 ccacgacctt cttgtctttt attgtctccg gtacatcccc ggcgaatatc cgaagcgcca
     1981 gcccctctgc aatggctgtt ttccccacac ctggctctcc tataaggacg ggattgttct
     2041 ttgtgcgccg cgagagtacc tggagcactc ttcgtatctc attgtcccgg cctatgacag
     2101 gatcgagttt tccaatagct gcaagttcag tgaggtcgcg tccatatttt ttgagggcct
     2161 cgtatttatc ctcaggattc tggtcagtga cgcgggatga gccccgcact tccttgaggg
     2221 atttcatgat agcgtccttt gatacgccgt tctctttcag gatgcgcgca gagatgccat
     2281 ttttctcatc agcgatagca agaagcagat gttcactgct caggtattca tcgcgaaggt
     2341 tcttcatttc aatggatgca acttcagctg cgttgttcaa tcgcgtggac atgtatatct
     2401 ggccggcgcc agcgccttcc acatgcggaa gcttgtcgat ctcctcttca agctttgaga
     2461 ttatcccccc ggtatttgcg ccgagttttt gcaggagctg gagcgttaag ccttctttct
     2521 ggttaatgag ggcaagtaaa aggtgctcta cttcaagctg ctgctggccc ctgtcaagcg
     2581 ctatggtctg tgcttcctgg aatgcttcct gtgctttgat agtgaatcgg tcgaatcgaa
     2641 ttgacatgtg tgttctctcc ctgagtgttt attggcggct gggggattaa aaggttgctt
     2701 ccggggattt tggagccgcc agggctgcat cattattttt gaaaacaaag agttaaattt
     2761 caatacaatc aaagaatgat cacacatgcc aaaaacaaac aggaccgccc cgctatacga
     2821 catgcttttt agcgaaggca aattcaacgg aaccctcctt atcggacctg acccacttgg
     2881 cgcaactttt gactacgggg tctttcttga agtcaggcga agactgagat tgatcgggct
     2941 cacgctgact gaaagcagac gtggatttga ggaatatgaa aaagcattca attttaataa
     3001 taggatcata cgtgcaagaa ttcgtctttt cctgggtcgc aattttggcg gggatctgca
     3061 ggaaatatgg cggcaaaccc tgacgaatga agatctcata tatctgaaaa cgcacgccgg
     3121 gtatggtaaa cacctgagcc tgagcgacga tgtgagttac tttgctgatg ccatgaaaga
     3181 aggattcgat cgtccaggga gaaaaccata ccagttgtac tatcttgatt gctgtaagag
     3241 tgaaatgtat tataaagatg tcttcaggaa atatgttgga ggcgtagacc ttattttgca
     3301 taaatggttt tgtgattaca ggataatcgg accggtagtt gtgctgataa gggaactgat
     3361 ggcaggttcg gattttgaga ccattatttt gaaaatgaat gaagaatatg gaattccaca
     3421 ctttgatgtg gaggatgatc ctgcggatat gagattggat agaaagatgg ttacatattc
     3481 tttaggtgat tctgttagac gaaattcgac ttaactttaa gaagatggca aagatgaaat
     3541 caaatgcgaa atggctctat aatgaaactg ttcaagtagg tacagactat caggatattt
     3601 caaatgtata aattgcttaa gaatgacggg actttttatt taagtgatgt ggttttttca
     3661 tttccaattg aagaatatga ctataaaatc gacaattgga ttgatactat gaaagaaaaa
     3721 gtgggaaaga attttgcaga ggaagcgcaa atgcatatta aggaagaata ttcgaccttc
     3781 gattggattt tagaggaaat gctccctaaa tcaggattca gatttgatat tcgtcataag
     3841 gatgattttt ccgctgtata ttactgcaag aagaagtgaa ttgttctgca atatcgtgaa
     3901 ctactcccct ttgaaaaggc gagcttcccg cttcattggt tctctattga gattacttgg
     3961 ccgggcttga agtcccgtag ttcctacggt actgattgat ttattcttga ttaatatcgc
     4021 tgacatgtga tctctgccaa gtaccaaccc acacaacgga caattatgta tcctcactga
     4081 caggtctttt ggtactctac acccacatat gcaggtctgc gaagttcctt tgggattaac
     4141 caattccact accttaccag cgtattctgc tttgtatttc gttaaattga ttaaattcga
     4201 ccatgatgca tcgtgtatgg actttgcaag atgatggttt tgaaccatgt tttgaatttg
     4261 cagg
//
LOCUS       DATGBN010000201         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_14790, whole genome shotgun sequence.
ACCESSION   DATGBN010000201 DATGBN010000000
VERSION     DATGBN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14790"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..641)
                     /gene="bfr"
                     /locus_tag="VKL21_04765"
     CDS             complement(162..641)
                     /gene="bfr"
                     /locus_tag="VKL21_04765"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HMB45115.1"
                     /translation="MKGSKKLLLVLNSLLADELTVVNQYMVHSEMCENWGYNKLHMAI
                     RKQAMDEMHHAEWLIERIIFFEGAPTVSKLNPIKIGKTVSEMISNDHDDELKVVHDYN
                     EAIRLAREVDDEGSVDLLTKILKMEEGHVDWGEIQRAQIEQMGMENYLVNQTESPAS"
     gene            complement(862..1116)
                     /locus_tag="VKL21_04770"
     CDS             complement(862..1116)
                     /locus_tag="VKL21_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45116.1"
                     /translation="MPPKLHVWQMFIKPDEIKSLLHENHLDWKEHCGIKPNISYLKML
                     RYLRKRAAGKLTYEEFGKKFLMVKSRSTRIMYMGYAVKQN"
     gene            complement(1158..>1585)
                     /locus_tag="VKL21_04775"
     CDS             complement(1158..>1585)
                     /locus_tag="VKL21_04775"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HMB45117.1"
                     /translation="CQMRQILITGATGNIGTEVIRSLTILGTNNQIIAGVRNILKAKQ
                     LFFDFKNLDYRDFDFENPQTFDKSLDNIDCIFLLRPPHISDVDKYFKPLIQKISEKGI
                     RQIIFLSVQGAEKSKIIPHNKIERLIRDSGLDFIFLRPK"
BASE COUNT          454 a          301 c          259 g          571 t
ORIGIN      
        1 atttttagtt tttactatcg aaagaaaaaa tctcgtgttc aagaaatgtg aggcccttat
       61 ttgattaatg ttgcactaat tttagtttac ttataggtag tcatcttatt gttagtcaga
      121 gcaagagcct gaagggatta tttcgattaa aagatgatgc atcagcttgc cggactttca
      181 gtctggttaa ccagataatt ctccatgccc atttgctcaa tctgtgcacg ttgtatttct
      241 ccccaatcga catgaccttc ttccatttta aggatctttg tcagaaggtc aacactgcct
      301 tcatcatcaa cctcacgggc gagtctgatc gcctcattat aatcatggac aactttaagt
      361 tcatcgtcat ggtcgttact aatcatttct gaaacagttt taccaatctt aataggatta
      421 agttttgata ccgttggtgc accttcaaaa aaaatgatac gttcaataag ccactcagca
      481 tggtgcattt catccattgc ctgttttctt attgccatgt gaagtttatt gtaaccccag
      541 ttttcgcaca tttcagaatg caccatatac tggttcacaa ctgtaagttc atctgccaga
      601 agtgaattca agactaacag taatttttta ctccctttca tagtttttct tttttaattt
      661 atatgcattt aagaattttt agaaaactaa gttaaacaca tgtgaccaaa taagtcttac
      721 ataacttctg aagaaagtta tataattcac atattttctg atgtgatgct cctttatgat
      781 tgaagtcttt ttataaagac cgattttcgg atatcagact cttgtgcttt ttggttggcc
      841 cgataggaaa aacagtaaat tttaattttg tttaaccgca tatcccatat acataatccg
      901 tgtactgcgg cttttaacca ttagaaattt tttaccaaat tcttcataag tcaattttcc
      961 tgcagccctt ttgcggagat aacgaagcat cttcagatag gatatatttg gtttaatacc
     1021 acagtgttct ttccaatcca ggtggttttc atgcaataat gattttatct catctggttt
     1081 tataaacatt tgccatacat gaagcttagg aggcattata gaccaccgct tccactcctg
     1141 caaaatcttg atagcactta ttttggtctc aggaagataa aatccagtcc tgaatctcgg
     1201 atgagtcttt caatcttgtt atgcgggata atcttgcttt tctctgctcc ctggactgag
     1261 agaaatatta tctgtctaat acccttttca gatattttct gaatcagagg tttaaaatat
     1321 ttgtcaacat ctgaaatatg aggtggtcta agcagaaata tgcagtcgat attatcaagt
     1381 gatttatcaa atgtctgagg attctcaaag tcaaagtccc gatagtcaag atttttaaaa
     1441 tcaaagaata attgttttgc ttttaaaata tttcgtactc cggcaattat ttgattattt
     1501 gtcccaagga ttgtgagact ccttattact tctgtcccta tgtttccagt cgccccggtg
     1561 atcaagattt gtctcatttg gcaaa
//
LOCUS       DATGBN010000202         6142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_365038, whole genome shotgun sequence.
ACCESSION   DATGBN010000202 DATGBN010000000
VERSION     DATGBN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365038"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..676
                     /locus_tag="VKL21_04780"
     CDS             167..676
                     /locus_tag="VKL21_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMB45118.1"
                     /translation="MEVLEALRTRRSIRKYTKEEIPEGDIRKIIEAAMSGPSAVNSQP
                     WHFIIVKSRSILNEIPKASPYAQMTKDAAIAIVVCGDPKLDKIPQFWPQDCSIAAQNI
                     LLAAHSLGYGAVWTASYPMEDRVKNLQKLLGIPSSIIPLAVIPIGKPAEIKEGDKPYL
                     EERIHLDKW"
     gene            complement(752..1825)
                     /locus_tag="VKL21_04785"
     CDS             complement(752..1825)
                     /locus_tag="VKL21_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HMB45119.1"
                     /translation="MNILSQYKLGDLELSNRMVMAPMTRCRAIEGNVPNPLAAIYYTQ
                     RASAGLIITEGSQVSKYGVGYVNTPGIHSPLQVAGWKKVTEAVHKAGGIIFLQLWHVG
                     RVSHPDFFRGELPVAPSALPVEGEVHTPQGKKKFVIPRALELDEIPGIIEQFRKGAQN
                     AKAAGFDGVEIHGANGYLLDQFLRDGSNRRTDKYGGSLENRLRLPLEVTEAVVGIWGV
                     DRVGYRISPHFSLYSMSDANPRQTFSHFAKELNDIGLAYIHIVEPVGGRMGATAPSAK
                     LAPIIHDIFEGTLILNGGYDSSSGNEDIEKGDADLISFGVLFLANPDLPERFRKNTQL
                     NPPDIATFYAGEEKGYIDYPKIG"
     gene            complement(1858..2505)
                     /locus_tag="VKL21_04790"
     CDS             complement(1858..2505)
                     /locus_tag="VKL21_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMB45120.1"
                     /translation="MKITWSIVLCLFVFILISGCLSSQDIGNETEATEYMGIKLTPIS
                     DQGNNAIKGTQFIDKDTYRLKIDGNVERPANFTYAQITNYTPVSKVVNLNCVEGWSFT
                     AKWTGVPVKTLLDRTGIKENATNVIFYSADGYSTSHDLDYLMDNNIMLAYRINDITLP
                     PDRGFPLQLVAEGKYGYKWAKWIDHIELVNSSYKGYWESRGYNNKGDFGGPPYEQ"
     gene            complement(2546..3433)
                     /locus_tag="VKL21_04795"
     CDS             complement(2546..3433)
                     /locus_tag="VKL21_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HMB45121.1"
                     /translation="MDNIVQVGVVRKKDIIQSVKTSVDLAGGMKIKKNSTVLIRPNAN
                     TADFPPGSTNPEVLKGAIRAVKEYNPAKIIVAEKSMTTLDTIKVMKHLGLYQAAESEG
                     VDEIVSFDDGKWKKVQHPYANSWHHGFSVPVILDTIDYMVALPIVKTHWTALFTIGLK
                     SQISVTSDLDRRQLPHGQKNDELFGTMIAESNLVHKPDFYISDATKCFVTDGPNVGTL
                     REPGIIIATSDVIANDVLGLALLKTLGTIDKIQNNSVWEQPQIKRAVELGLGVKNRNE
                     IQIKSDGVKEIENIIVNIL"
     gene            complement(3481..4026)
                     /gene="msrA"
                     /locus_tag="VKL21_04800"
     CDS             complement(3481..4026)
                     /gene="msrA"
                     /locus_tag="VKL21_04800"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMB45122.1"
                     /translation="MHQSHEIATLGGGCFWCLEAIFSELRGVMNVISGYSGGNVPNPT
                     YQNVCTGATGYAEVIQVTFDPVIISFKELLEVFFSIHDPTTRNRQGADMGTQYRSVIF
                     YHTIEQEKAAREIIEQIDEAKIWGAQIVTELTPFTEFYPAEEYHQEYLKRNLDKMYCQ
                     AVIAPKLSKFHKQYFMKLKKG"
     gene            4350..4802
                     /locus_tag="VKL21_04805"
     CDS             4350..4802
                     /locus_tag="VKL21_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMB45123.1"
                     /translation="MIATDGSEHTEKAIKHAIELAKLTGAQLHAVYVISIISPPENLD
                     IKTDYETELSVSVDASMEGLKKILELDGDKAIRSIEEQAAIKGVNVRKWILEGQSAKE
                     ILRLAEEQSFDLIVMGTLGRSGIEKFLLGSVADKVIRGSNIPVLTVRK"
     gene            4905..5564
                     /locus_tag="VKL21_04810"
     CDS             4905..5564
                     /locus_tag="VKL21_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45124.1"
                     /translation="MIDKTTARKLSRYVHEVQISIDGIRSHDMLRGKGTYTRYMKGIS
                     NLNESGIPVSIATMVHKFNMDEFDEMEKLFSKMGAISWSVDVPCIIGRLCENMDFVLD
                     LKEAAKFLKYGFGAGGHESTGDHTCGSHLCSVASDGSVSKCGFFENEPAGNVNDLGSA
                     WENICKSSLRRLDELECKDCAVLHECKGGCRFRAKQFKGILAPDPLMCHANLPGGYFH
                     G"
     gene            5557..>6142
                     /locus_tag="VKL21_04815"
     CDS             5557..>6142
                     /locus_tag="VKL21_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMB45125.1"
                     /translation="MDNVDVIIIGAGVGGLTAAANLARTGRKVIVLEQDSHIGGTAHV
                     FWRDGFTFPTGPQSVTMPAYIADSLCELGVQRRLSFMRDHFQVIRGSMNIIISEPLHH
                     VSGQLLDHYPKECKGIHLVIKVLEEVITALDVLQPYDLIEQGSGCITDSNACAVLERW
                     GSIPARDLLDCHLKDQSLKDLLGSQGTGETEMSVV"
BASE COUNT         1755 a         1281 c         1340 g         1766 t
ORIGIN      
        1 tttctaatta cgggatcgaa ttataccagg ccgcagatga cggactaaac aaatgggtca
       61 tggctgcaga tgaaggaaaa gtgggacgcg caaggttgat cgggaaaaag atgtgaggag
      121 gcaaaaaaaa accataaaca ggatttatta aatggaggta tgaaaaatgg aagtcttaga
      181 agcattgcga acaagaagat cgataagaaa atatacaaaa gaagaaatcc ccgaaggcga
      241 tattagaaaa atcattgaag ctgccatgag cggtccatcg gcagttaact cccaaccctg
      301 gcatttcata attgtaaaaa gtagatccat tcttaatgaa ataccaaaag caagccctta
      361 tgcacaaatg acaaaagatg ctgctatcgc catagtagta tgtggagatc caaaactcga
      421 taaaattccc caattctggc cgcaggattg cagtatcgcc gctcaaaata ttcttttagc
      481 agctcactct cttggctatg gggcggtatg gaccgcatct tatccaatgg aggatcgtgt
      541 taagaacctg cagaaactcc tgggtatccc atcatccatc attccgcttg ccgttattcc
      601 aataggtaaa cctgctgaaa taaaggaagg cgataaacca tatctggaag agaggattca
      661 tctggataaa tggtagataa atggattatc acaagaaatt gccggatatt tttgtggagt
      721 gtgatggtag tgttttgtgg tattttctta attatccaat tttcggatag tcgatgtaac
      781 ctttctcttc gcctgcataa aatgtcgcaa tatcaggtgg atttagttgt gtatttttcc
      841 tgaagcgttc cggaagatca ggatttgcca gaaacaaaac tccgaatgag atcaggtcag
      901 catccccttt ttcgatatct tcatttccac tgctggaatc gtagcctcca tttagaatca
      961 atgtcccttc gaatatatca tgaatgatcg gagccaattt cgcagacggc gctgtagccc
     1021 ccatccttcc tcctacaggc tccactatgt gaatatacgc aagtccaata tcgtttagtt
     1081 cctttgcaaa atgtgaaaag gtttggcgcg gattggcatc agacattgaa tataatgaaa
     1141 agtggggcga aatcctgtaa ccgactctat caacacccca tatgcccacg accgcttctg
     1201 tgacttcaag gggaagacgt aagcgattct caagacttcc accatattta tcagttcgcc
     1261 ggtttgagcc gtctctcagg aactgatcca atagatatcc attggcgccg tggatctcaa
     1321 cgccatcaaa gcctgcagct tttgcgttct gtgcaccttt tcggaattgc tcaattatgc
     1381 caggaatctc gtccagttcg agagctcttg gaatcacaaa tttctttttg ccctgcggtg
     1441 tatgtacttc gccctccaca ggaagcgccg aaggtgcgac aggcagttcc cctctgaaga
     1501 agtctggatg tgatactcgt cctacatgcc agagttgaag gaaaattatt cctcctgcct
     1561 tatggactgc ttcggtaact ttcttccagc ctgcaacctg aagaggtgag tggattcccg
     1621 gtgtattgac atagcccact ccatacttgc tcacctgcga tccttccgtg attatcaaac
     1681 ctgccgatgc tctttgtgtg taatagatag cagctaacgg atttgggaca ttgccctcga
     1741 ttgcgcgaca acgggtcata ggcgccataa ccatgcggtt tgagagttca agatcgccca
     1801 atttatattg agataaaata ttcatatcta ctccttctac ttatgattat atgtctgtta
     1861 ttgttcgtat ggaggccctc caaaatcacc cttattgttg tatcctctgc tctcccaata
     1921 acctttgtag gatgaattta caagttcaat atgatctatc cattttgccc acttatatcc
     1981 atatttgcct tcagccacaa gctgcagagg aaatcctctg tccggaggaa gagttatatc
     2041 atttatcctg taagccaaca tgatgttatt atccattaga taatcaagat catgggatgt
     2101 tgaatagcca tctgcactgt agaaaataac attggtggcg ttttctttta tcccggttct
     2161 atcaagcagt gttttcacgg ggacacctgt ccactttgcg gtaaaactcc atccctctac
     2221 gcaattcagg tttacgactt tagaaactgg agtataattc gtgatctggg catacgtgaa
     2281 gtttgcgggt ctttctacat tgccatcgat tttcagtctg taggtatctt tgtcgataaa
     2341 ttgggtgcct ttaattgcat tattaccctg atcgcttatg ggtgtgagct ttatacccat
     2401 gtattctgta gcttcggtct catttccaat gtcctgactt gaaaggcacc ccgaaatcag
     2461 gataaataca aagaggcaca gtacgatcga ccaggttatt ttcatgaaac ttcttttctc
     2521 ctgagtaatt aagaaaaatt taaatctata aaatattgac aataatgttt tcgatttctt
     2581 tcaccccatc acttttgatc tgtatctcat tcctgttttt tactccaaga ccaagttcta
     2641 cagccctttt tatctgaggt tgctcccata cggaattgtt ctggatttta tcgattgtac
     2701 ctagtgtctt caaaagtgcc aggcccaaca catcattcgc gatcacatca cttgtagcaa
     2761 tgataatacc aggttcccgc aaagttccga cattgggtcc atctgtcaca aaacatttcg
     2821 tggcatctga tatgtaaaaa tcgggtttat gtacaagatt tgactctgcg atcatagtac
     2881 caaagagttc atcgtttttc tgtccatgcg gaagctgtct cctatcaaga tcggaggtaa
     2941 cgctgatctg ggattttaac ccaatagtaa acaatgcagt ccagtgtgtt tttacaatgg
     3001 gaagtgcaac catataatcg atcgtatcta ggatcacagg tactgaaaaa ccgtgatgcc
     3061 atgaatttgc gtatggatgc tgcactttct tccatttccc gtcgtcaaaa gatactatct
     3121 cgtccacacc ttctgattca gccgcctgat atagacccag atgtttcatc accttgatgg
     3181 tgtccagtgt ggtcattgat ttctcggcta cgataatctt tgcagggttg tattctttga
     3241 ctgcacgaat agcaccttta aggacttcag gattggttga tccaggcggg aaatcagctg
     3301 tgttcgcatt cggtctgatc agaacagtag aatttttctt aattttcatg cctccagcaa
     3361 ggtcaacaga ggtttttaca gattggatta tatccttttt cctgacaaca cctacctgga
     3421 ctatattatc catttttctt ttcatctcct taattataat ttcataatgg tcaagaggag
     3481 tcatcccttc ttgagtttca taaaatattg tttgtgaaac ttagacaact ttggcgcaat
     3541 cactgcctgg cagtacatct tatcaagatt ccgcttgagg tattcctggt gatactcctc
     3601 ggccggatag aactcggtga aaggtgttag ctcggttacg atctgagcac cccagatttt
     3661 cgcttcatct atttgctcta ttatttctct tgcagctttt tcttgttcta ttgtgtgata
     3721 gaagatcact gatcgatatt gagtgcccat atctgctccc tgtcgattgc gtgtcgttgg
     3781 gtcatgaatg ctgaagaata cctccaggag ttctttgaat gagatgatca ccgggtcaaa
     3841 cgtgacctga ataacctcag catagccagt tgcacctgtg cagacatttt gataggtcgg
     3901 gttgggaaca ttgcctcctg aatagccgga aattacattc ataacaccac gcagttcgct
     3961 aaaaattgcc tccagacacc agaaacaccc tccaccaagt gttgcgattt catgactttg
     4021 atgcatagat tgcagtcctc gtgcagttat ttcagcttaa tggaaaactc aaaatttgat
     4081 attgatccac taaccaaaat tttaatacaa ttaaataatc aatcaataag ttttctgtat
     4141 cttataggta tctggcgcag gtcactgtcg ctgtacccaa aatcaaaaaa gcaggattta
     4201 cgccaaatca tgatgaatcc agggtcaaaa aagaatgagg agtttattta ttcaagaatt
     4261 cacatggaaa aacatataag caaaaatgaa catagaaatt ttgcacataa tcagggggaa
     4321 aaattacgag cgatctttat aaaaaaatcc tgatagcaac agatggatca gagcatactg
     4381 aaaaggcgat aaaacatgca atcgaattag caaagctcac gggggcacag cttcatgcgg
     4441 tttacgtgat aagtattatt tccccgccag aaaatctgga tattaagacc gactatgaaa
     4501 ctgaattatc agtttctgtc gatgcatcca tggaaggatt aaagaagatc cttgaattgg
     4561 atggcgataa agcaataaga tccatagagg aacaggcagc gataaaaggc gtaaatgtca
     4621 ggaaatggat acttgaaggg cagtcagcga aggagatact gaggcttgcc gaagagcagt
     4681 catttgatct gattgttatg ggcacgctgg gcagaagcgg gatcgaaaaa ttcctgcttg
     4741 gaagcgtggc agataaagta attaggggtt caaatatccc tgtgttaacg gtgagaaaat
     4801 agatattgga ggtgaaaaaa atgaagatca taaagataaa aaagattgcc agaggaacct
     4861 gtaaatgcca ttcttcctgc acataagccc tctcaaacgg aactctgata gacaagacca
     4921 cggctagaaa attatccagg tatgttcatg aagtgcagat cagtatcgac ggtatccgtt
     4981 ctcatgacat gttgcgagga aaaggtacgt ataccaggta catgaaaggt atatccaact
     5041 taaatgaatc cggaataccc gtttcgatcg ccaccatggt acataaattc aatatggatg
     5101 agtttgatga gatggaaaag cttttttcta aaatgggagc gatctcatgg agcgttgatg
     5161 ttccatgcat cataggcagg ctttgtgaaa acatggattt tgttcttgat ttgaaagaag
     5221 ccgccaaatt cttaaaatac gggtttgggg ccggcggaca tgaaagcaca ggagatcata
     5281 cctgtggttc tcacctttgt tcagtagcgt ccgatggttc agtcagtaaa tgcggatttt
     5341 ttgaaaatga accagcagga aatgttaatg atcttggatc tgcctgggag aacatctgta
     5401 aatccagctt acggagactt gatgaacttg aatgcaaaga ttgtgcagtg cttcacgaat
     5461 gtaaaggtgg atgcaggttc agggctaagc agtttaaagg aatacttgcg cctgatcctt
     5521 tgatgtgcca tgctaattta ccaggtgggt actttcatgg ataacgtgga tgtgatcatt
     5581 attggggcag gagttggtgg tctgacagcc gcagctaatc ttgcccggac aggacgcaaa
     5641 gtgattgttc ttgagcagga cagtcacatt gggggcactg ctcatgtctt ttggcgcgat
     5701 ggtttcacct ttccgaccgg cccacaatca gtaaccatgc ccgcatatat cgctgattcc
     5761 ctttgcgaat tgggggtgca aagacgtttg agttttatgc gggaccactt tcaggtaatt
     5821 aggggttcaa tgaacataat tatctctgag ccccttcatc atgtttcggg gcaattattg
     5881 gatcattatc ccaaagagtg caagggaata catttagtca taaaagttct tgaagaggtg
     5941 ataacagccc ttgatgtgct gcagccatat gacctcatcg aacaggggtc aggatgcatt
     6001 acagatagta acgcctgcgc ggtgcttgaa agatggggaa gcattccagc ccgggatctt
     6061 ttagactgcc atctgaaaga ccagagcctg aaggatctcc tgggaagcca gggaaccggt
     6121 gaaaccgaaa tgtcggtagt gc
//
LOCUS       DATGBN010000203         9032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_365460, whole genome shotgun sequence.
ACCESSION   DATGBN010000203 DATGBN010000000
VERSION     DATGBN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365460"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VKL21_04820"
     CDS             <1..1145
                     /locus_tag="VKL21_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45126.1"
                     /translation="IAIVSIGNGMQQALNARLSGLGGDIVTISPGVERGGGMFGMRGG
                     GGGGYGDSSGSQATTKAIVLGRSDLQALKGIPDISLIDTNIRGNVNMSYLGKTGKVSV
                     TGVDQKVWSQITTIAIKDGRMLDSADQNVIVIEGGLASSYFSQPVGINKMVMINGSAF
                     RVVGILDDQGNSVYMPIQMAYQVIPDKTNDIYDTLVVKIKNQDQLDGVITKIQDKLMT
                     ARHVTQKNMDFSVTSRKEMQQARADTMNSMYTFLLAIAAVSLIVGSIGIANTMFTSVL
                     EKTKEIGIMKAIGARKHDILSIFLFNAAFIGLVGGILGIILGTILSGYMPVLMGGLPM
                     ARGTAIVTFNSIAMALSVSVTVGILAGIIPAYQASKLKPVDALRYE"
     gene            1243..2124
                     /locus_tag="VKL21_04825"
     CDS             1243..2124
                     /locus_tag="VKL21_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HMB45127.1"
                     /translation="MYEIGVGDIHTHTMYSGFTKYKFLTFPDSVTTPRTSIRVAEKLG
                     LNALCITDHNTIKGALVAQKFNKDLVVVGEEISSKEGEILGLFLQEKVNPGLSAEETI
                     EQIHSQDGIAIAPHPYSGHCSCVTDKINTLRFDGIEVFNSLHRDGYSNAIALEKCNGH
                     AKFGGSDAHASFMIGNGYTLFNGSSQEDFRSSIKDRKTIYGGNVASIMDFVYYGARVA
                     YESSKTILKLNGVDCPMSARISRVSDSRKMSYFLGCLAFAFTPLPIVCTLLGDRVFKN
                     KGLRMWREIHNLPLRKE"
     gene            complement(2321..2842)
                     /locus_tag="VKL21_04830"
     CDS             complement(2321..2842)
                     /locus_tag="VKL21_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45128.1"
                     /translation="MVKKSEERKDIHIEDINTENKDIFKTWENSYTAILKMWEDSYLN
                     LYKPWLGSRGELFDKAVELSKEAFENFVRNASVDLSLTKSWIAILEKLSQKARELSKQ
                     NADTETYKEFNNLWAKTYEKAFENFFENIPTVSPFKEIFESVKNAAKIYAETFTSIFS
                     NRMKSYSISASSV"
     gene            complement(2900..3082)
                     /locus_tag="VKL21_04835"
     CDS             complement(2900..3082)
                     /locus_tag="VKL21_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45129.1"
                     /translation="MDIFDVLTAISKRKMAFMQENMNENKALIKAEVEISKEYHISLV
                     DIQKLLGVRFIPTGLV"
     gene            complement(3379..3735)
                     /locus_tag="VKL21_04840"
     CDS             complement(3379..3735)
                     /locus_tag="VKL21_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMB45130.1"
                     /translation="MSNEELLFELLANKYSRKILSLASIKECSATLLNQELGIPLATV
                     YRKVKLLEDAGFLQHVKTIINLQGNEEKYYRCAIREAIVHINGGEFSIDIKKEDRSDK
                     IIRLWRRLAHPDSKDI"
     gene            complement(3749..3928)
                     /locus_tag="VKL21_04845"
     CDS             complement(3749..3928)
                     /locus_tag="VKL21_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45131.1"
                     /translation="MKSKATAMLYLTFGFTLMTIGDLASAIYYLEDLHMYKVVSQTFD
                     ILGLAALIIAVEKVT"
     gene            complement(4255..4893)
                     /locus_tag="VKL21_04850"
     CDS             complement(4255..4893)
                     /locus_tag="VKL21_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45132.1"
                     /translation="MKLIPSGISWAYFVNLKQGTGIEESYMPDADFYGIQIIGMLNSE
                     YSENSWVELQDVGKSVASVPSRAGNDTDNILSTRPFIFSTKDRTSSVEALIKESGNAT
                     AYNTYSFLLEKVDDERAGYAKINTSAPSFANASYIGLISSGNDITGEIAFSIKDKAVV
                     PLETYNNLKNSSSGRGFRSYDIMLENNTLIIRMTSNLNNVLSEATQNYGIDI"
     gene            complement(4944..5153)
                     /locus_tag="VKL21_04855"
     CDS             complement(4944..5153)
                     /locus_tag="VKL21_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45133.1"
                     /translation="MKCNESDHEFGGNAKFCPENKYLKLMNKNRTIISIVAVTLFIVT
                     LGCIGSTQDKIAISPLPTPQKRFRR"
     gene            complement(5230..5928)
                     /locus_tag="VKL21_04860"
     CDS             complement(5230..5928)
                     /locus_tag="VKL21_04860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB45134.1"
                     /translation="MISVIIPTYNEEANIIACLQSLCNQTIRRDEYEIIVVDGNSKDC
                     TCELAREYADEVITQTSKKVGGARNDGVLRARGDIVATTDADCIIPPEWLEIIKNDFK
                     SHDIVQLYGTVYPIEGRLKHKIYLMTGNTFSRIGYYTGGLYYTLGCNTAFDKKAFIEA
                     GMYQCIDAGDDLEIALRMRKLGRVMLDGRMKVGFSMRRFQQFGMIKSVYEWLYIVAKG
                     GEASKYSYSKRNYR"
     gene            complement(5925..6902)
                     /locus_tag="VKL21_04865"
     CDS             complement(5925..6902)
                     /locus_tag="VKL21_04865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB45135.1"
                     /translation="MRINIFAEDFMNLKYLGCGTAAKTLYRQLSGMPDMDVSWNSKGR
                     DFDIVHYHTFGPVSLLSLRYSQGIKILTVHVTPRLNEGGLAFSKYITRYYPYSYRKFD
                     HIITLVDGCRSEVREIAPDVPTTTIPNGVDRNYFKMDEGKRKSFREQYKISDSEKVVL
                     AVGQQTPRKGIYDFIELAKLHPEIRWVWVGGFPAGPFSKDRLKIEWMKKKCANNVIFT
                     GFVPDIAQAYSGSDVFFMPSYSEMFVLVILEALSCGLPVVARGIPEFKEIFGGAVQFF
                     NNIEEAGERISDEVLLKHYSNGSRDFTEQYDIKKVANMHCDLYRKLIES"
     gene            complement(6899..8212)
                     /locus_tag="VKL21_04870"
     CDS             complement(6899..8212)
                     /locus_tag="VKL21_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMB45136.1"
                     /translation="MKTAILGGGITGITLARLLAQKGVEVTVLEREPVFGGLCRSISD
                     SGFTFDRGGSHIIFSRDAEVLTFINNILQENSQRNIRNTKIFYKERYIKYPFENGLNQ
                     LPKEDLFFCINEFIKTLIAVERGKLAPPGNFREWIIHTFGKGIADLYLIPYNEKIWKF
                     PTEQMSLHWVEGRIPRPPVEDIIKSAIGIETEGYTHQSVFSYPRKGGIEALVRSIASP
                     VEKSIRTDFDVSSIKKEGEKYLISDGDNIIIADRLISTIPLQRLLKSLENVPEGVRKS
                     CNELKYNSIACVFIGVRGKIQDISWMYVPDKDMGLFNRVSFPSNYSPDVAPAGHSAIL
                     AEITYHEGDDVSKMSDQKIIDHVIKALKNMNIVKDREDIVYTALERQTYAYVIYDLDY
                     KKNIATVKDFCAKLGIGLVGRFSEFEYLNMDGCIRNAMNFVRDHK"
     gene            8325..8891
                     /locus_tag="VKL21_04875"
     CDS             8325..8891
                     /locus_tag="VKL21_04875"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMB45137.1"
                     /translation="MKKSLGAKTLVYPLPVWVVGTYDKESKPNVATIAWGGICCSQPP
                     CVAISLRKATYSYGNIVERKAFTVNVPSEKYVTEADYFGSVSGRNVDKFTESGLTSVK
                     SEFVDAPYVKEFPLVLECKLIHTIEIGLHTQFIGEIMDVKADELVLGEKGITDIEKLK
                     PVIFAADNGGYYGIGKSIGKAYSIGKKR"
BASE COUNT         2589 a         1866 c         1749 g         2828 t
ORIGIN      
        1 taattgcgat agtttcaata ggcaatggaa tgcagcaggc attgaacgct cgtcttagcg
       61 gtcttggcgg agatattgta acgatctcac caggcgttga acggggcggg ggcatgttcg
      121 gtatgagagg agggggtgga ggaggttatg gagattcctc cggttctcaa gcaacgacca
      181 aagcaatagt actgggcagg agtgatctac aggcattgaa gggtattcct gatatttcgc
      241 tgatagacac aaatatcagg gggaatgtga acatgtcgta tcttggtaaa acaggtaaag
      301 tatccgtaac aggtgttgat cagaaagtat ggtctcaaat tacaacaatt gcaataaaag
      361 atggccggat gcttgattct gccgatcaga atgtcatagt tatcgaagga gggcttgcat
      421 cctcttattt ttcccaacct gtgggtataa ataagatggt catgataaat ggcagcgctt
      481 ttagggtcgt gggaattctg gatgatcagg gcaatagtgt ctatatgccc atccagatgg
      541 cataccaggt tattccagac aaaacgaatg atatttatga cacattagtg gtaaagatca
      601 agaaccagga ccagctggat ggagtgatca cgaaaatcca ggacaagctt atgactgcaa
      661 gacatgttac tcagaaaaac atggactttt cagttacatc aaggaaggaa atgcaacagg
      721 caagggcaga tacgatgaat tcgatgtata catttcttct tgctattgca gcggtttcat
      781 tgatcgtagg ttctatcggg atagcaaaca ccatgttcac ttctgtcctt gaaaagacca
      841 aagaaattgg gatcatgaaa gctattggtg cgcgaaagca tgatattctc tccatattcc
      901 tgtttaatgc tgcttttatc ggactcgtgg gagggatcct gggcataata ctgggaacaa
      961 ttctttcggg ctacatgcct gtccttatgg gaggtcttcc tatggcaaga ggtactgcaa
     1021 tagtcacatt taattcgata gctatggctt tatctgtgtc agtaacagtt gggatactcg
     1081 caggaataat tccagcatat caggcatcaa aattaaagcc agtggatgca ttgaggtatg
     1141 aataatgttt gacgtttgaa agttctaaac atgtgaaaaa cttgaagcag ttcccttaaa
     1201 atttttatac aataagacta ataagataag tatgggggaa atatgtacga aataggagtc
     1261 ggggatattc atacacacac catgtattca ggcttcacga aatacaaatt tttaacattc
     1321 cctgacagtg taaccactcc caggacatct atcagggttg ctgaaaaact ggggctcaat
     1381 gctctttgca taactgatca caacacgatt aaaggtgcgc ttgttgctca aaagttcaat
     1441 aaggacctgg tggtggtcgg tgaagagata tcttctaaag agggcgaaat actgggatta
     1501 ttcctccaag agaaagtcaa tcctggctta agcgccgaag aaacgatcga acaaatacac
     1561 agtcaggatg ggatcgctat tgctccacat ccttatagtg gacattgttc atgtgtgacg
     1621 gacaaaataa acacattaag gtttgatggg atcgaagtat tcaattcgtt gcacagggat
     1681 gggtattcaa atgcaattgc acttgaaaag tgtaacggtc atgcaaagtt tggcggaagt
     1741 gatgcgcatg caagtttcat gatagggaat ggttatacat tatttaatgg cagttctcag
     1801 gaagattttc ggtcttccat aaaggataga aagacaattt acgggggaaa tgtcgcatct
     1861 attatggatt ttgtctatta tggagcaagg gtagcatatg aatcttccaa aacaatcctg
     1921 aagttaaatg gagtagattg ccccatgtct gccaggatat ccagggtaag cgattcgcgt
     1981 aaaatgtcat attttctggg gtgtctcgca tttgcattta caccattgcc cattgtttgc
     2041 actcttctgg gtgatagggt tttcaaaaac aaaggactta gaatgtggag agaaatacac
     2101 aatcttcctt tgagaaagga gtgattttaa ttggcacagg tttgtgaatc cataattatg
     2161 gaatttagtt ctgggattgg atacaattcg ccaaatgtat tctgttattt tttaatccat
     2221 tcattataga attgttttct tgggagatat tggaatattt ataattccct tttcccacgg
     2281 tgcaaaaaaa ataacattct ctgccaattt cctggatgga ttaaaccgaa cttgcagata
     2341 tggaatacga cttcatccta ttactgaata tgctggtaaa tgtttctgca tatatctttg
     2401 ctgcattctt tacagactca aagatctctt taaacgggct tacagtggga atgttctcaa
     2461 agaagttttc aaacgccttt tcatatgttt ttgcccagag attattaaat tctttatatg
     2521 tctctgtatc agcattttgt tttgatagtt ccctggcctt ctgtgacagt ttttcaagga
     2581 tggctatcca ggacttggtg agactcaaat caacgctggc gtttcgcacg aaattctcaa
     2641 atgcttcttt tgagagttcg accgctttat caaacagctc tcctctggat cctaaccagg
     2701 gcttgtatag atttaagtag gaatcctccc acatttttaa aatagccgta tagctgtttt
     2761 cccatgtctt gaaaatatcc ttattttctg tattgatatc ttctatgtgg atatcttttc
     2821 tttcttctga tttcttaacc atatttattc acttcttttt atagtccccg tatttaaata
     2881 cgggatcttt ctttatcttc taaaccagtc cagtgggaat gaatctcact cccaacaact
     2941 tctggatatc aaccaatgat atgtgatatt ctttcgagat ctcaacttca gccttgatca
     3001 aggctttatt ttcgttcata ttttcttgca tgaatgccat tttcctttta gaaattgctg
     3061 tcaaaacatc aaaaatatcc atgtgtcacc aacctccggc tcagcatttc ttctaacata
     3121 tcgtctacat ttagcaagta acatgctttt tccatatcta tctcactaag gatatcatat
     3181 gcttccattt ctctcgaaat tttccgttta tagttcattt ctgtcacctc ttgattaagc
     3241 attcttacta tctttctgga ttataaatgg tataatccac cctcagaaga tgagaattat
     3301 gatatgacca ttcccggaat atgagaaccg caggtgcatt gaccttccca ccagtagccg
     3361 aattcactta tagtgttttc agatatcctt gctatccggg tgggcaagcc ttctccatag
     3421 tctgataatt ttgtcgctgc gatcttcctt cttaatatcg atagaaaact ccccaccatt
     3481 gatatgcact atagcctcac ggattgcgca gcgataatac ttctcttcat taccctggag
     3541 atttattatc gtcttgacgt gctgtaaaaa ccccgcatcc tctaaaagct tcaccttcct
     3601 gtaaacagtc gcaaggggta taccaagctc ttgattcaac agggtagctg agcattcctt
     3661 tatagaagcc aaagacaata tctttcttga gtacttattt gccaaaagct caaaaagtag
     3721 ctcttcatta ctcatgtttg atatcggtct aagtaacttt ttccaccgct attattagtg
     3781 cagctaaccc aagtatatca aaggtctgag aaacaacctt gtacatgtgc aggtcttcta
     3841 aataataaat agcagaggct aaatctccta tggtcattaa ggtaaagccg aaagtgagat
     3901 aaagcatggc agtggctttt gatttcaaaa acgctcggta tgataagatc attagaataa
     3961 aacctgcaag aaaacgaaca gcagtgaatg agtgatcgat taagtatgtt ggtatcatat
     4021 ttttcacgga atccttggtt tccgattctt tttatatgac tatagcatta atctaaagat
     4081 aatgatttgt tctattctca aagtttgaga gtgggctatt ccatttatct tctaaaaaag
     4141 taagtaaata gatgaggagc ctttttagct atcctatcac ccaaaaaaaa attccttttt
     4201 tggctcctct aacaccctgg ttctaaatcc ccttgcaaga tttaaaaatt atccttaaat
     4261 atcaattcca taattttgtg tagcttctga aagaacatta ttcagattcg aggtcattct
     4321 aataattaat gtattattct ccaacataat atcgtatgac ctgaagccac gacctgaact
     4381 tgagttctta aggttgttgt aagtttccag aggtaccact gctttgtcct taatgctgaa
     4441 tgctatttct ccagtaatat cgttcccaga acttataaga ccgatgtaac ttgcatttgc
     4501 aaaagaaggc gcactggtat ttattttcgc atatcctgct ctctcgtcat caactttttc
     4561 aaggaggaag ctatatgtgt tgtatgcagt tgcgttgcct gattctttaa ttagcgcctc
     4621 cacgctgctt gttctgtctt tcgtgctgaa tatgaatggt cttgtagaaa gtatgttatc
     4681 ggtgtcattc cctgctcttg acggaacact ggcaaccgat tttccaacat cctgaagttc
     4741 aacccacgag ttttctgaat attctgagtt caacattcct attatttgaa ttccgtaaaa
     4801 atccgcatcc ggcatataac tctcctcaat tccagttccc tgtttaaggt tgacaaaata
     4861 agcccatgag atgccagatg gaattaactt caaaccatcg cttaaggact tgaagttatc
     4921 aggtagtgag agggatgaca ggtctatctg cgaaaccgtt tctgaggtgt gggcaaagga
     4981 gatatagcta ttttatcctg agttgaacct atgcatccca gagtaacgat gaataatgtc
     5041 actgcaacta tcgaaattat agtcctgttc ttgttcatta atttcaaata cttattttca
     5101 ggacagaatt ttgcattgcc accaaattca tgatcggact cattacattt cataaattat
     5161 ctttagttat accctgaata aagttatcga tttgttcagt accattcaat tctttacaca
     5221 tggatggatt tatctataat ttcttttcga gtatgagtat ttcgaagctt cacccccttt
     5281 ggcaacaatg tatagccatt cataaaccga ttttatcatc ccaaattgct ggaatctgcg
     5341 cattgagaat ccaaccttca tacgaccatc aagcattaca cgcccaagtt tacgcatcct
     5401 cagtgcaatc tcaaggtcat cacctgcatc gatgcactgg tacatgccag cctcaatgaa
     5461 cgctttttta tcaaatgcag tgttgcatcc aagcgtataa tacaggccgc cggtataata
     5521 cccgatcctt gagaaggtat ttccagtcat aaggtagatt ttgtgtttta atctcccttc
     5581 aattggataa acggtcccat aaagttgaac aatatcatgg gacttgaaat cattctttat
     5641 tatctcaagc cattcgggag ggataatgca atctgcatcg gttgtagcaa cgatatctcc
     5701 tctggcgcgc aatacaccgt cattcctggc cccacctacc tttttgcttg tctgggtaat
     5761 aacttcatcg gcatattccc gtgccagttc acatgtgcag tccttagaat ttccatccac
     5821 tacgatgatt tcgtattcat ccctcctaat tgtctggtta cagagggatt gcaggcaagc
     5881 tataatgtta gcttcttcat tgtatgtggg gataattacc gatatcatga ttcaatcagt
     5941 ttcctgtaaa gatcacaatg catatttgca acttttttaa tgtcatactg ctctgtgaag
     6001 tcacgcgacc cattagaata atgttttaat aatacctcat ctgaaatccg ctcccctgct
     6061 tcctctatat tattaaagaa ttgcacagca cctccaaaaa tttctttgaa ttccggtata
     6121 cctcgggcaa ctacaggcag gccgcatgat agagcttcca gtatcaccag cacaaacatc
     6181 tcagaatacg aaggcatgaa aaatacatct gagccactgt aagcttgcgc gatgtcaggg
     6241 acgaatcccg tgaatattac attatttgcg catttctttt tcatccactc tatcttgagg
     6301 cgatcttttg aaaagggccc ggcaggaaat cctccaaccc aaacccagcg tatttcagga
     6361 tgcagttttg ccagttcgat aaaatcgtat attcctttgc gtggtgtctg ttgacctact
     6421 gcaagcacta ccttttcgct gtcggatatt ttatactgtt ccctgaagct tttccgcttg
     6481 ccttcatcca tcttgaaata atttctatcc accccatttg ggatcgttgt ggtaggaaca
     6541 tcaggggcaa tttcccgaac ttcgctccgg cacccgtcca ccagtgttat tatatgatca
     6601 aacttcctgt agctgtatgg gtaataacga gtaatatatt tgctaaatgc cagcccccct
     6661 tcattaaggc gtggagtaac atgcacagtc agtatcttta tgccttgtga atatcgcagg
     6721 ctcaagagcg acacaggtcc aaatgtatga taatggacta tgtcaaaatc acggcctttt
     6781 gaattccatg atacgtccat atccggcata ccggacaact gcctgtaaag tgtttttgct
     6841 gcagttccac aaccgagata ctttaaattc ataaaatctt ctgcaaaaat attgatcctc
     6901 atttatgatc cctcacaaaa ttcatagcat ttctgataca gccatccata ttaagatatt
     6961 caaattccga aaaacgtccc acgagtccta tgccaagttt tgcgcaaaaa tcttttactg
     7021 ttgcgatatt ttttttataa tcgaggtcat agataacata cgcgtaagtt tgccgctcca
     7081 gggcagtata tactatatcc tctctatcct ttacgatatt catattttta agtgctttaa
     7141 tcacatggtc aataatcttt tgatcagaca ttttggatac atcatcccct tcatgataag
     7201 ttatctctgc aagtatcgcc gaatgtcccg caggcgccac gtccgggctg taatttgacg
     7261 gaaatgaaac cctgttaaat aaccccatat ctttatcagg aacatacatc cacgagatat
     7321 cctgaatctt tcctcgcacc ccgataaaaa cacaggctat cgagttatac ttaagttcat
     7381 tgcaggattt tcgaacacct tccggaacat tttcgagact tttcaagagc ctctgtaaag
     7441 gaatcgtaga tattaagcgg tctgcaataa taatattatc gccatcactt attaaatact
     7501 tttctccttc tttcttgatt gaagatacgt caaagtctgt gcgaatgctc ttttcaactg
     7561 gtgatgcaat tgatctcacc agcgcttcaa tcccgccctt tcgggggtag ctgaaaacgg
     7621 actggtgcgt atatccttcg gtttctattc cgatagcaga ttttataata tcctccactg
     7681 gcgggcgggg aatgcgccct tctacccagt gcaatgacat ttgctctgtt gggaatttcc
     7741 agatcttttc attatatggg attaagtaca ggtcagcaat acctttcccg aatgtatgga
     7801 tgatccactc acgaaaattc cccgggggcg caagtttacc tctttcaaca gcgatcaggg
     7861 ttttaataaa ttcattgata cagaaaaaga gatcctcctt tggaagctgg ttcaacccat
     7921 tttcaaatgg atatttgata taccgttcct tatagaaaat ttttgtattg cgaatattgc
     7981 gctggctgtt ctcctggaga atattattta tgaatgtgag gacttccgca tcacgactga
     8041 aaatgatatg tgagcctccc ctatcaaaag tgaaccctga atcgctaatg gagcgacata
     8101 atcccccaaa aaccggttcc cgttcaagaa ctgtcacctc aacacctttt tgcgcaagta
     8161 agcgggcaag agtgataccg gtaatacccc cacctaatat tgctgttttc acatcgaata
     8221 attcgctatc aatccctata ttgttatcta tttgacaaaa acccaccgaa gtgttaaaat
     8281 atctataaac tagaacttac tattacttac ataaggagga atttatgaaa aagtctttag
     8341 gtgcaaaaac tcttgtttat cctctcccgg tctgggttgt gggaacttat gacaaagaaa
     8401 gtaaacctaa cgtggcgact atagcatggg gtgggatatg ttgttcccaa ccgccatgtg
     8461 ttgcgatctc tcttcggaaa gcgacatatt cgtacggaaa tatcgtggag cgaaaagctt
     8521 tcactgtaaa tgttccatcc gaaaagtacg tcacggaagc ggattatttc ggttctgtct
     8581 caggcaggaa cgtggacaag ttcaccgaat cgggacttac cagcgtgaag agcgagtttg
     8641 tcgatgctcc atatgtgaaa gaattccctc ttgtcctgga gtgcaaactg atccacacta
     8701 ttgaaatagg gctgcacacg cagtttatcg gtgagatcat ggatgtcaag gcagatgagc
     8761 ttgtacttgg ggaaaaaggg attactgata tcgaaaaatt aaaaccggtc atatttgctg
     8821 ctgataatgg gggttattat ggaatcggta aatctattgg aaaggcatat tctattggga
     8881 aaaagagata gcgggctgcc agagcaaata cccaatatta catatccttt caaatcataa
     8941 tattatatct aataatggca ttattagata tatttgtata ttatggcatg ttcggtcttt
     9001 ttatcgtcag ttttttgtcg tccatcatac ca
//
LOCUS       DATGBN010000204         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_93571, whole genome shotgun sequence.
ACCESSION   DATGBN010000204 DATGBN010000000
VERSION     DATGBN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93571"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VKL21_04880"
     CDS             complement(<1..327)
                     /locus_tag="VKL21_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HMB45138.1"
                     /translation="MTTDLKGIIEKIKYPMVVISILAAFLLALYIRAVLPHDSVFLGN
                     GVISISEDYGVYQMRIVEWIVHNFPDFLWFDPYTLYPHGQNMPWAPFFTYSLAIIALI
                     AGLGSPT"
     gene            complement(492..3053)
                     /locus_tag="VKL21_04885"
     CDS             complement(492..3053)
                     /locus_tag="VKL21_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HMB45139.1"
                     /translation="MTTDLKDIIEKIKYPMVIISILCAFILSLYIRTVLPHDSVFLGN
                     GVISISEDDGVYQMRIVEWIVHNFPHFLWFDPYTLYPHGQTMPWAPFFTYSLAIIALI
                     AGMGSPTLDTIYTVGAYYPAILGALVVIPTYYIAKNVFDDRRIGILSAFIIALLPYQF
                     LDRSILGFTDHHIAETLLSTVTMLYLILSVKVARKNSFTFSDIKSLKWRALKPSIYYF
                     ILTGLFLSLYSLTWKGALLFAFIIGIYIMVQHIVDHMHNKPTEYLGVLGTIIFLIPLI
                     IVIIVPDIGGEKNLYYVGLTAGIILFPLLSGLSILLNKKGYEKYFYPLSLAILIIGGL
                     LFIYAAMPPIFDTLKNSLTFFSRSGGGLTIGEAWPYPFTFEAFKDDFGINGVLAFIGL
                     AFLAYGVFKKERHEKTVFLVWTIMMLWALYQQNRFAYYYAVNVAVLSAYFGIAVADKI
                     LEFGDWNELLGKKGIKRADTPKKKDIKKASKTHISKPQDPNGFDFKKIKVTHIISLLS
                     ALFLVAYLVYPLIEPATIRGKQGTPMRTYWYESLTWMRYNTPDPGIDFYGKYQEPTVG
                     VNYSYPDSAYGVMSWWDYGHVITAWAHRIPNANPFQSGIGGGSTHAPGASTFLSAQSE
                     EEANAVLDALGVNGKPGARYVITDDYINFYVFGAVLEWAKIPGDGYYSQVRTNKGYQT
                     VPYTKRYNAMMMRLHLFDGDGLKTYRLVHESISASHYPDREVGMDEEGFKDVYNKLYG
                     GNLPVEYTGHVKIFEHVKGAYITGKAPANATVSISLKVKTNQGREYSYSQKTVSNGEF
                     ELIVPYSTEGPISGETNFDTKPIGKYILTAGNISREVSVNERTVIDGGTEKVDLV"
     gene            3165..3890
                     /locus_tag="VKL21_04890"
     CDS             3165..3890
                     /locus_tag="VKL21_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB45140.1"
                     /translation="MELLKNISDQIQTNLTSNFHIRARYLHDLYRPLLEKVPNKNNYM
                     DLGCGFGVNSAVFGEGFNIVYCSDLSMTELGICRKNMREKKNYHYIAADAQSLPFKNE
                     YFDLVTAFSLIEHVPCQEKMLREAFRILKNQGQLVLQFPNKHFFMELHTGIPFFCLVP
                     DFMKTWILEKKSYDGFLNIPTPEQVKDTIHVIDPSVNVRVVNVIYPDDLVPVNFKKVY
                     SLLKKTGIFRKIPFGWLVICEKP"
     gene            complement(3918..4753)
                     /locus_tag="VKL21_04895"
                     /pseudo
     CDS             complement(3918..4753)
                     /locus_tag="VKL21_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612368.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         1449 a          976 c          923 g         1405 t
ORIGIN      
        1 tgtgggggag cccagaccgg ctatcagcgc tattatggca agactgtatg tgaaaaacgg
       61 cgcccagggc atattctgtc catggggata taatgtgtag gggtcaaacc agaggaaatc
      121 cggaaaatta tggactatcc attccactat cctcatctgg taaacaccgt aatcttcgga
      181 aatagagatc acgccatttc caaggaaaac actatcatgg ggtaaaaccg ctcgtatata
      241 aagagcgagc aggaatgccg caaggatcga tataactacc atcgggtatt tgattttttc
      301 aattatgcct tttaagtctg tggtcatgat ttgcctttag atattgaatt tctaataata
      361 ctgattccat tggatataag tattctgaga taaattaaaa gggtgattac caacgcctgc
      421 ctaaaaccat tggtaactta tttcatacta ttgtaacaaa ttacaatgaa tctatgaatt
      481 ggtaaccacc tttacacaag gtcaactttt tccgtgccgc catctatcac cgttctttca
      541 ttcacagaaa cctcgcgtga aatattgccg gcagtcagta tatatttccc aattggtttt
      601 gtgtcaaaat ttgtctcacc ggatattggc ccttcggtgg agtaaggcac gatcaattca
      661 aattcgccat ttgaaactgt cttctgggaa taggaatact cacgtccctg gtttgtcttt
      721 actttcaggg aaatagaaac cgtcgcgttt gccggggctt ttccggtgat ataggcgcct
      781 tttacatgtt cgaatatctt cacatggccg gtatattcaa ccggaaggtt gccgccatat
      841 aatttattat atacatcttt aaatccctct tcgtccatcc caacttccct gtctggataa
      901 tgagaagcag aaatagattc atgcaccagc ctgtacgtct tcaagccatc accgtcgaac
      961 agatgaaggc gcatcatcat tgcattgtat ctctttgtgt atgggacagt ttgataccct
     1021 ttatttgtcc gcacctgtga ataatatcca tcaccaggga ttttcgccca ctccagaaca
     1081 gcgccaaaaa cgtaaaaatt aatataatcg tccgtgatca cataacgggc gcctggttta
     1141 ccattcactc ccagcgcatc aagcacagcg ttcgcttctt cttcagattg ggctgataaa
     1201 aaggtggaag cgcccggcgc atgtgtcgat cctccgccga ttcctgactg gaaaggattt
     1261 gcatttggta tccggtgagc ccatgctgtt atcacatgac cataatccca ccagctcatg
     1321 accccataag cagagtcagg ataagaataa tttaccccaa cggttggttc ctggtacttc
     1381 ccgtagaaat caatgccggg gtctggtgtg ttataccgca tccaagtaag ggattcatac
     1441 cagtaggttc tcatgggtgt tccttgtttt cctctaatcg ttgccggttc gataagtgga
     1501 tatacaagat atgcgaccag gaataaggcg gacaagagcg atataatatg ggtgactttg
     1561 atctttttga agtcaaaacc attcggatcc tgcggtttgg atatatgcgt ttttgatgct
     1621 ttcttaatat ctttcttttt aggggtatca gctctcttaa tacccttttt tccgagcaat
     1681 tcattccaat caccgaattc aaggatttta tctgcgactg ctattccaaa ataggcggaa
     1741 aggacagcca catttactgc gtagtaataa gcaaacctgt tctgctggta taatgcccaa
     1801 agcatcatta ttgtccatac aagaaaaacc gtcttttcat gtctctcttt cttgaaaacc
     1861 ccataagcta aaaaagccaa tccgatgaat gcaagaacgc cgtttatccc gaaatcatcc
     1921 ttgaaagcct caaaagtaaa aggataaggc catgcctctc cgatcgtcag gccgccgccg
     1981 gaacgtgaga aaaaagtgag ggaatttttc agggtatcaa agatcggagg cattgcggca
     2041 taaatgaaaa gaagtccgcc aataataaga atcgccaaag atagaggata aaaatatttc
     2101 tcatatccct ttttatttaa taagattgat aatccgctca acagtggaaa gaggataatc
     2161 ccggcagtca aacctacata ataaagattc ttttcccccc cgatatcagg aactattatc
     2221 acaattatca gggggatcag gaatataata gtacccagaa cccccagata ttctgttggt
     2281 ttattatgca tatgatccac aatatgctga accattatgt atatcccgat aataaaggca
     2341 aatagcagcg ctcctttcca tgtgagtgaa taaaggctca ggaaaagacc tgttaatatg
     2401 aaataatata ttgaaggttt caaggctctc catttaaggg atttaatatc agaaaaagta
     2461 aagctgttct ttcgcgcgac tttaaccgat aggataagat acagcatcgt gacagtactg
     2521 agcaaggtct ctgcaatatg atgatctgtg aaaccgagaa tggatctatc taagaactga
     2581 tatggtagga gagcaatgat aaatgcagaa agaatgccaa tccgcctgtc atcaaaaaca
     2641 ttctttgcaa tataatatgt tgggataacc acaagagcac ccagaattgc cgggtaataa
     2701 gcgccgaccg tatatattgt atcaagtgtg ggagagccca taccggctat cagtgctatt
     2761 atggcaagac tgtatgtgaa aaacggcgcc cagggcatag tctgcccatg gggatataat
     2821 gtatatggat caaaccagag gaaatgcggg aaattatgga ctatccattc cactatcctc
     2881 atctggtaaa caccgtcatc ttcggaaata gagatcacgc catttccaag gaaaacacta
     2941 tcgtgtggta aaaccgttcg tatatacagg gagaggatga atgcacaaag gatcgatatg
     3001 attaccatcg ggtatttgat tttttcaatt atgtctttta agtctgtagt catgatttgc
     3061 cttttagata ttgaatttct aatattacta attccattgg atataagtat tttgagataa
     3121 attaaaaggg atatatctaa aatatctaga aataagttga tccaatggaa ttattaaaaa
     3181 acatatcaga tcagatccag acaaatctta cttcaaattt tcatatacgg gcaagatatc
     3241 ttcacgatct atatcgacca ttattggaga aagtaccaaa taaaaacaat tatatggatt
     3301 tagggtgtgg attcggggta aattcggcag tttttggtga aggatttaat attgtctatt
     3361 gttctgatct tagcatgact gaacttggaa tatgcaggaa aaatatgcgg gagaaaaaga
     3421 attaccatta catagctgca gatgcacagt cccttccatt caagaatgag tactttgatc
     3481 ttgtgacagc gttctcgctg atagaacatg tgccttgcca ggaaaaaatg ctcagggaag
     3541 ctttcaggat attgaaaaat cagggacagc ttgttctgca attcccgaac aaacattttt
     3601 ttatggaact gcatacaggg atcccttttt tctgtcttgt ccctgatttt atgaaaacat
     3661 ggatactgga aaaaaaatca tatgacggtt ttctaaatat accaacgcct gaacaggtca
     3721 aggatactat tcacgtaatt gacccttctg tgaatgtcag agtagttaat gtaatatatc
     3781 cggatgacct tgtgcctgta aatttcaaaa aagtgtattc attgctcaaa aagaccggaa
     3841 ttttcagaaa aatacctttt ggctggcttg tcatatgcga aaagccatga atatgaaaac
     3901 gcttcatagg tgttcgctta agctatccat gaatccatca atctctttct attaatgttc
     3961 ggatcgcgcg cctgcattct cagtaaacac ttttgcccat agtaaatgcg tataccgata
     4021 agcattttca ggattcactt tttttatcaa tctgagtatt tggcgagaac atataagagt
     4081 caggatagct acccagataa ggcatttcac tacattcgga tttgcactct cgatctgatc
     4141 aatgtgatat acatctttta attctttgaa aagaagttca acctcccatc ttgcactata
     4201 aaggtcagca atatcctcac caattaacac atcgattggt atatttgtca aataaacatg
     4261 atatttttca gtctcctcgt taagaacaca taccattcgg aaggttctgg tcatttttgt
     4321 agttttgctc ttatattttc gtcttttgat ctctacctca acagttacat caagcatttg
     4381 tcttttcaaa agcggaagta cattttttag ctttttccct acaacatcaa ttgagttccc
     4441 cctgcatatt tggttcactc taacaattgt tggattggca tttcctttca atctgctcac
     4501 aaaaaagcca ccatatcgtt cgattttatc aaaggaatta taatcaaaga aaccaaggtc
     4561 ggtcagaaga atgcggtctt tgagccatga acctaatctg agagtcttca cttcagctgt
     4621 cctttcagag tagagcctta cagttttaac gccatctgcc actgcactaa ctatacaaga
     4681 tatacaagaa agtttcagtc cagcagctat ctttttagag cgggcagcag gtcatagctt
     4741 cgcaagtgac tca
//
LOCUS       DATGBN010000205         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_171346, whole genome shotgun sequence.
ACCESSION   DATGBN010000205 DATGBN010000000
VERSION     DATGBN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171346"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VKL21_04900"
     CDS             complement(<1..364)
                     /locus_tag="VKL21_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMB45141.1"
                     /translation="MTVIDANNLILGRMASTIAQRILNGEEINVINAEKAIISGRRDA
                     TFDRYKAYAARGSREFGPRFPRRPDQIIARTVRGMIPHTKTTGREAYSRLRVYIGVPP
                     ELSKEQAITINDASITRLS"
     gene            complement(369..731)
                     /locus_tag="VKL21_04905"
     CDS             complement(369..731)
                     /locus_tag="VKL21_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HMB45142.1"
                     /translation="MKNNYKTNARLLDLISGLKEQSRDKKVPLWRDIALRLERPTRNA
                     AEVNLSRINRYTKEKDLILVPGKVLGAGELNHQLTVAAVSFSEGARNKIIEAGGSCLS
                     IEELMNKNPEGSRVRIIG"
     gene            complement(1121..2275)
                     /locus_tag="VKL21_04910"
     CDS             complement(1121..2275)
                     /locus_tag="VKL21_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45143.1"
                     /translation="MNTILKLIALVFALIMLTFPVLGEMMGTGNEMMRGKAKEMYEKG
                     SQMRENEGVRGMGFMHSQGNSFGNYVTFSVDNKTGDVMNYSILGTPIFDSIKVSGSNF
                     KETATGGAVTWITNNDGSIVIQLHDNPAAVINIMAKAKASIIFDLAEGANAAKEDNIV
                     KISSDNLTAYIASGNATSVNIDGANVSIGIDKGFTIFRASPVNMPMSEMHRGFMGEIN
                     RNRAGAEISVGDFDESSIVNYSKDMNVMIKSMEKNRMRMMVNSSDHSGKFIMMNIDNS
                     SLMWNEGQKLRLYLDNKPMRQVMGEQELYNATESSFWLTMMGRNRMEAIMYVANFSER
                     QVDIVVEDEATPTPEVTRTTTATTTPGTPGFEVALGMLCVAAAYGLRRRG"
     gene            complement(2558..2631)
                     /locus_tag="VKL21_04915"
     tRNA            complement(2558..2631)
                     /locus_tag="VKL21_04915"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2595..2597),aa:Ile,seq:gat)
     gene            2758..2829
                     /locus_tag="VKL21_04920"
     tRNA            2758..2829
                     /locus_tag="VKL21_04920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2790..2792,aa:Ala,seq:ggc)
     gene            3347..4096
                     /locus_tag="VKL21_04925"
     CDS             3347..4096
                     /locus_tag="VKL21_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HMB45144.1"
                     /translation="MSKNVLLDDGRNDIRKAESAHERGDSKLAMELYLKAITKFRNAA
                     EITDDFSEISIIRSLISYYMNRASLLESEPGHIEVKKPRAKENARNDITELLRGTGVN
                     ETVFEAVIEIALEISTEGREGRSIGTAFILGDSVNVLAKSRQLIFNPFQGYKKEERLI
                     TNPDMLDNIKEFAQLDGAFVITGDGAVEAAGRYITIDTGMVKLPKGLGTRHNSIATIT
                     RWTDSIGIVVSQSGGVIRIFRNGLVAATVRP"
BASE COUNT         1191 a          935 c          834 g         1314 t
ORIGIN      
        1 tgctaagacg tgtaatactt gcatcattta tagtgatcgc ttgttcttta ctaagttcag
       61 gaggaacccc aatataaact cttaatctgc tataggcttc tcttcctgtg gtttttgtat
      121 gcgggatcat acccctgacg gtccttgcta ttatctggtc gggtcttctc ggaaatcgag
      181 gtccgaactc acgtgaacca cgggcagcat aagccttgta ccgatcgaat gtggcatctc
      241 ttcggcctga gatgatcgct ttttcagcgt taatgacatt gatctcctca ccatttaata
      301 ttctctgtgc tattgtgctt gccattcttc ccagaataag attatttgca tctatcactg
      361 tcatgttatc acccgattat ccttaccctg gagccttccg ggtttttgtt catcagttct
      421 tctattgaga ggcatgaacc tccagcttct attattttgt tccttgcgcc ttcgctgaaa
      481 cttacagctg caacagtcaa ttgatgattc aattcacctg cgcccaggac tttgcccggt
      541 acaaggatca ggtctttctc ttttgtgtac ctgttgatcc tgctgaggtt gacttctgca
      601 gcatttctcg ttggacgttc aagccttaac gcaatatcac gccatagtgg gacctttttg
      661 tccctggatt gttctttaag acctgatata aggtctagca atcttgcatt tgttttgtaa
      721 ttatttttca ttcttttcct ccgtaagacc atttatggtc cgactcatgc aatcctttaa
      781 ggttttactg cattcggatt gtttcaatcc atacgccgca ggcttttgcc tgctacggtt
      841 aatattgaac cacaaaaatt tgattcagat tgccttaatt cctttttaaa tacataaact
      901 taccgtagtg attcaggaga ttttttaatt tattccggag aaatggttca tcacaaagaa
      961 aaacaaccac agagtccacc aagagcacag agcatagacg cttttctctg tatgctctgg
     1021 tgtgaaaaaa ttaacaacga actcgtacga actccataag aattcacatt gtcggagtta
     1081 gttttagttc gttgttttcc agatgtttct cgataccgga ttatcctctt ctcctcaatc
     1141 cataggcagc ggcaacacac agcattccca gcgccacctc aaatccgggt gttcctggcg
     1201 ttgtagttgc cgttgttgtt cttgtcactt caggagtcgg agtagcttca tcttcgacca
     1261 ctatatccac ctggcgctcg gagaaatttg cgacatacat aatcgcttcc atgcggtttc
     1321 tccccatcat tgtaagccag aatgacgact cggttgcatt atataactcc tgctcgccca
     1381 tgacctgcct cattggttta ttatcaagat ataagcgtag cttctgccct tcgttccaca
     1441 tcagtgagct gttgtcaata ttcatcatta tgaatttacc ggaatgatca gacgaattta
     1501 ccatcattct catgcggttc ttttccatgg actttatcat gacattcatg tcttttgaat
     1561 agttgacgat cgaggattca tcaaaatccc ctacggaaat ctcagccccc gccctgttcc
     1621 tatttatttc tcccatgaat ccccggtgca tttcactcat gggcatattt acgggcgaag
     1681 cccgaaagat cgtgaatccc ttatcgatcc ctatactgac attggccccg tcaatattta
     1741 ccgatgttgc gtttccggaa gcgatatatg ccgttagatt atctgatgag atctttacaa
     1801 tgttatcttc tttggcggca ttggcccctt cggccagatc aaaaattata ctggcttttg
     1861 cctttgccat gatattgatc acggcggctg gattatcatg aagctggata acgatcgaac
     1921 catcattatt cgtgatccat gtcactgccc cccctgttgc tgtttccttg aaattggatc
     1981 ccgatacttt tatggaatca aagatgggtg tgcccaggat gctgtaattc attacatctc
     2041 ctgttttatt atcaaccgag aaggtcacat agttgccgaa gctatttccc tggctgtgca
     2101 tgaaccccat tcccctcacg ccctcatttt ctcgcatctg gctgcctttt tcatacattt
     2161 cttttgcttt tccacgcatc atttcattgc ccgtacccat catttcgccc aggaccggga
     2221 aagttagcat tatgagtgca aacactaacg ctattagttt caatattgta ttcatatatt
     2281 atcccataac tccaaaacaa atatgtgact ttcaggtaat agacttttcg gattataatt
     2341 ttgcgcataa taatcgataa attataaata ttgatgaata aatacagcgg tattttttca
     2401 tttattttgc attagagaag aaccgcagat aaacgcggat gaacgcagat ttaatgcttc
     2461 gtatcaagaa aatatgggta gattaaataa taataataaa aaattgtaat tatctgtggt
     2521 tttcataagt ttagtggaag actattgacc aaatgcatgg gcccgttgcg atttgaacgc
     2581 aagacctctc ggttatcagc cgagcgctcc acctggctaa gctacgggcc cgagaatacc
     2641 gcccatatta cgccatccga tttaaacctt atctttgtaa aggtaatatc cagccgaatg
     2701 aactcgaact aatcgatacc tttatctaag aataagcact atgcaactca ccctttaggg
     2761 ccggtagctc aggggtagat cgctcccttg gcatgggaga ggcctcgggt tcaaatcccg
     2821 accggtccat aaatcatgta ttcattttaa ggttttttcg atctcagatt tcaagatata
     2881 aaaaaatccg gtagtataat gagatcaaag aagatattgc atttgggtga ttttttcaat
     2941 ctttcaagac cgatgttacg actttttaga gacttgattt atgcctttaa attaaaaaat
     3001 cttaatatag atatcacaat tggatttaat gatcctgctt ataccggcat tttaacagga
     3061 ttcctgcatt caatccctgt tcaggcggga cattccatca ggtggactgc cgacttcaca
     3121 aagtcggtcc tggaatggaa tctgaaagca gaagctgcca ttacaccaat ccatatatta
     3181 ctgcatatgg caagattttt aacgagcaga caagttttga aattatacaa atctttttta
     3241 tgctaattta attaaaattt ttggattgtt cctttgacct ttatccttat aatattagaa
     3301 cgcttattat attgagacta acgaatggaa actgaagtga tctgaattga gcaaaaacgt
     3361 tcttctggat gatgggcgaa atgatattag aaaggctgaa agtgcgcatg aaagaggcga
     3421 ctctaagctg gctatggaac tctacctcaa ggcaattaca aaattcagga atgcagccga
     3481 aattacagat gacttcagtg aaataagtat tatcaggagc cttataagtt attacatgaa
     3541 ccgtgcatcc ttacttgaaa gtgaacctgg ccacattgaa gtcaaaaagc cccgggcaaa
     3601 agaaaacgcc cggaatgata taacagagtt gttaagagga acgggggtaa atgagacagt
     3661 ctttgaagct gtgatagaaa tagcgcttga aataagtacc gaaggaagag aaggccgctc
     3721 tattggcaca gcttttatat tgggagattc tgtaaatgtc ctggcaaaat ccaggcagct
     3781 tatatttaat cctttccagg gatataaaaa ggaggaaaga ttgataacaa acccggatat
     3841 gctcgataat atcaaagaat ttgcgcagct ggacggcgcg ttcgtaataa caggcgatgg
     3901 cgcggtagaa gctgccgggc gttatattac aatagatacg ggaatggtaa aactgccaaa
     3961 aggcttgggc acgcgtcaca attccatagc aactataacc aggtggactg attcgatagg
     4021 aatagtagtt tcacaaagcg gcggtgtaat acggatattc aggaatggtc ttgtagccgc
     4081 aactgtaaga ccgtgacatc ctccctcacc tttcgggagg gaacttctat acccatagac
     4141 ctcacgaggt taaaaaaaaa cccgatttca aactctcgaa acattcaagc tctatattga
     4201 cctttgagta ccgaaaaaaa aatattgaaa tcctgaggat actaaccaat gttagaaatt
     4261 cgacctttca aagc
//
LOCUS       DATGBN010000206         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_248837, whole genome shotgun sequence.
ACCESSION   DATGBN010000206 DATGBN010000000
VERSION     DATGBN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248837"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="proS"
                     /locus_tag="VKL21_04930"
     CDS             complement(<1..1021)
                     /gene="proS"
                     /locus_tag="VKL21_04930"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMB45145.1"
                     /translation="MAETKEKATQDKKPVLPSKSNFSEWYNELLLMAQIMDVRYPVKG
                     LYVWFPFGFDVRKRTYSIIRELLDKDHQEALFPLLIPENEFMKEAEHIKGFENEVYWV
                     THGGKNELDVKLALRPTSETAIYPMYKVWVRSHADLPIKIYQIVNTFRYETKHTRPLI
                     RLREITSFKEAHTVHATWDEAAQQVKEATMIYQEFYRRLAIPTIVSKRPDWDKFPGAD
                     YTMAVDTLMPDNKTLQIGTAHHLGSNFAKTFDIKYEDINGDQVLAHQTCYGISERSIA
                     ALISVHGDDKGLVLPPEIAPIQVVIIPILFGKSDETLNACHKVEERLKARNMRVALDE
                     TDERPG"
     gene            complement(1084..1590)
                     /locus_tag="VKL21_04935"
     CDS             complement(1084..1590)
                     /locus_tag="VKL21_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HMB45146.1"
                     /translation="MFSLHFYHADQCDPKKCTGKKLARFNLAKLHKNPRTLPRQAIFL
                     DPFAIQALSPADDAKNGIVVLDCSWEEVERVFPILQRLMMRHRALPYLLAANAVNFGK
                     PFKLSSVEAFAAALYILGEKEQAALILNKFKWGPIFLELNQEPLERYSEARDSTEVVK
                     IQADYLPS"
     gene            complement(1605..2705)
                     /locus_tag="VKL21_04940"
     CDS             complement(1605..2705)
                     /locus_tag="VKL21_04940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMB45147.1"
                     /translation="MFSQRVKSIDISGIRRMFEGAGPNSINLGLGQPDFDTPLHIKDA
                     AIEAINKGLTGYTMNLGFPELRSALSRKFESENHFMVSQDEVIVTSGASEALHLALMA
                     LVDKGDEVLIPDPGFVSYSALTKISGGKIVGVPLGEKLTMTPEAVNELITPGTRVIIV
                     NSPSNPTGTVQTRKEIKALAQIADDNDITIISDEVYEHFIYEGEHVSPAQFTDNVITI
                     NAVSKTYAMTGWRIGYAAARKEYVEQMLKVHQYIQACANSIAQKAAQAAIEGPQDCVK
                     QMRDSFRNRRDIIMGGLDGMGIKCVKPEGAFYAFPEVGDDDAPQKLLKNGVIVVPGSA
                     FGENGKGHIRLSYATSEDKLRRALAVMETVLG"
     gene            complement(2710..>2978)
                     /locus_tag="VKL21_04945"
     CDS             complement(2710..>2978)
                     /locus_tag="VKL21_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HMB45148.1"
                     /translation="KLSVITSNRDSTLGRISDIVLIVPGRPKEDVVYEDYHERRMIGY
                     PQLAPLGTIFEISALVFLDAVISELMVRTGASEAELKKRHTVFE"
BASE COUNT          738 a          721 c          633 g          886 t
ORIGIN      
        1 ctcccgggcg ctcatcagtt tcatcaagag cgaccctcat atttcttgcc tttaatcgct
       61 cttcgacctt gtggcatgca tttaatgttt catcgctctt cccgaatagt attggaatta
      121 tgacaacctg gataggggct atctcaggcg gcaataccaa tcctttgtca tccccgtgta
      181 ctgatatcaa cgctgcgata cttcgttccg aaatgccata gcatgtctgg tgcgcaagta
      241 cctggtcacc attaatatcc tcatatttaa tatcaaatgt ctttgcgaaa ttgcttccaa
      301 ggtgatgcgc cgtccctatc tgcagggtct tattatcggg catcagggta tccactgcca
      361 ttgtgtaatc cgcacccggg aatttatccc agtcagggcg ttttgaaaca attgtgggaa
      421 ttgccagtct cctgtagaat tcctggtaga tcattgtagc ttcctttacc tgttgcgcgg
      481 cttcatccca tgtcgcatga acggtgtgcg cttctttaaa ggatgtgatc tcacgaaggc
      541 gtatgagagg acgtgtgtgt tttgtttcgt acctgaaggt gttcactatc tggtaaatct
      601 taataggcag gtcggcatga gaacggaccc atactttata catgggataa attgccgttt
      661 cgcttgtagg acgaagtgca agtttgacat caagttcatt ttttccgccg tgcgtgaccc
      721 aataaacttc attttcaaaa ccttttatgt gttctgcttc cttcataaat tcattttcag
      781 ggataaggag gggaaaaagc gcttcctgat ggtctttatc aagaagctca cggattattg
      841 aatatgttcg ttttctcaca tcgaacccga aggggaacca gacatatagc cctttgacag
      901 gataccttac atccattatt tgggccatta aaagaagttc attgtaccat tcgctgaaat
      961 ttgattttga gggaaggaca ggttttttgt cctgagttgc tttttctttt gtttctgcca
     1021 tggatgtcac cggtctggat gtgctagtat agttttatgg gctataaatt tttttaatgc
     1081 gaattatgaa ggcagataat ccgcctggat cttcacaacc tcagtgctat ccctggcttc
     1141 tgaatacctt tccagaggtt cctgattcaa ttcaaggaat atgggccccc atttgaattt
     1201 attgagaata agcgctgcct gttctttttc cccgagtatg tagagcgccg cggcaaaagc
     1261 ctctactgag ctcaatttga aaggcttacc gaaatttacg gcatttgccg caagaaggta
     1321 tggaagggcg cggtgcctca tcataagtct ttgaaggatc ggaaatacgc gttcaacttc
     1381 ttcccaggag cagtcaagga ctacgatacc gttctttgca tcatcggcag gagagagcgc
     1441 ctgtatggca aaaggatcaa ggaatatcgc ctgccttgga agtgtcctgg ggtttttatg
     1501 gagctttgca agattgaacc ttgcaagctt tttaccggtg cacttcttgg ggtcacactg
     1561 gtctgcatgg taaaaatgaa gagagaacat attattttaa aaatctatcc caatacggtc
     1621 tccatcacag caagcgctct ccttaattta tcctcggagg tcgcatacga taacctgata
     1681 tgtcccttcc cattctcacc aaaagccgaa cccggaacca caatgacgcc atttttcagc
     1741 aatttttgcg gcgcatcatc atcaccaact tcggggaatg catagaacgc cccttccggc
     1801 tttacgcatt taatccccat cccatccaga cctcccataa taatatctct cctattcctg
     1861 aaactatccc gcatttgctt cacgcaatcc tgcggccctt caatggctgc ctgcgctgcc
     1921 ttctgggcga tcgaatttgc gcatgcctgg atatactggt ggacttttag catctgctcc
     1981 acatattcct tccgcgcagc tgcatatccg atgcgccagc cggtcatggc atatgtcttt
     2041 gagaccgcgt ttattgtaat cacattatct gtgaactgcg cggggcttac atgctcacct
     2101 tcataaatga aatgttcata cacttcatca gaaattatcg taatatcatt atcatctgca
     2161 atttgcgcga gcgctttaat ttcttttctt gtctgtaccg tacccgtagg atttgagggt
     2221 gagttaacta tgatcacacg cgtccctggc gtgatcaatt catttaccgc ctcaggggtc
     2281 atggtcaatt tttcgccaag agggactcca acgatttttc ccccggatat ctttgtcaat
     2341 gcgctataag aaacgaatcc cgggtccggt atcaaaacct catccccttt atcgacaagc
     2401 gccataagcg caagatggag ggcctccgaa gctccggaag taactatgac ctcatcctgc
     2461 gataccataa aatgattctc agattcgaac ttccgggaaa gggcggaccg caattccgga
     2521 aagccaagat tcattgtata accggttaag cctttattga tagcttcgat ggccgcatct
     2581 ttgatgtgca gcggtgtatc aaaatcaggc tgtccaagac caaggttgat cgaatttgga
     2641 cctgctcctt cgaacatcct gcgtattcct gagatgtcaa tgctttttac gcgttgtgag
     2701 aacatgattt tactcaaaaa ccgtatgcct tttcttcaat tcggcttcgc ttgctcccgt
     2761 cctgaccatc agctctgaga tcacagcgtc aagaaaaaca agcgcagata tctcaaaaat
     2821 cgttccaagg ggagctaact ggggataacc tatcatcctt cgttcgtgat aatcttcata
     2881 aacaacatcc tctttgggtc ttcccggaac tatcaaaaca atatctgaaa ttctacccag
     2941 ggtagaatcc ctgtttgatg taatgacgga aagttttg
//
LOCUS       DATGBN010000207         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_132994, whole genome shotgun sequence.
ACCESSION   DATGBN010000207 DATGBN010000000
VERSION     DATGBN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132994"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VKL21_04950"
     CDS             complement(<1..727)
                     /locus_tag="VKL21_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45149.1"
                     /translation="MKPGYILTIILASVLILSFAIVQLNKDVLQKEPSYPSYLSIREV
                     DVKPVEVTSARIEVNVTAYINHNGGKTRDAVMLIRAINSETRLLESQVSAPIPETDSR
                     LDKTLSVSTNINVERNGGYELNILLFDNGSIRDSGTVNILGLNALIPGSKRSGVSLTN
                     IDFTVGGVSAGKVSIKSDIYLENKGPDVSDNLKMIVKAREATSNLIADKKNAETGTII
                     NESTVVRTVELEVPEGYNYMVVVE"
     gene            complement(803..952)
                     /locus_tag="VKL21_04955"
     CDS             complement(803..952)
                     /locus_tag="VKL21_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45150.1"
                     /translation="MLETSQERIELLKAGFTGNMVEKLYVECNNLKIERTPIIVELLE
                     LDISE"
     gene            complement(1095..1580)
                     /locus_tag="VKL21_04960"
     CDS             complement(1095..1580)
                     /locus_tag="VKL21_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45151.1"
                     /translation="MTTNSHEIVNSITNHGIPVFHTPEIHIKPFLCRKSVCNLADIIA
                     VFKQSERFYIVGIEIKEWNALVNSKLALSYLETYRDTCEYFYLAAKRFSRATFEIKDL
                     GLFDLNTMKVIKDPAYLFPDPDFRANLMKRIKKHYKVLEGVVEDPFQRTLLEFQIHNG
                     L"
     gene            1739..1882
                     /locus_tag="VKL21_04965"
     CDS             1739..1882
                     /locus_tag="VKL21_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45152.1"
                     /translation="MKNMDMLWSGLARRASEYTRIERKSQFSGVHEIAFKSTKVQDQS
                     KDI"
BASE COUNT          596 a          430 c          444 g          729 t
ORIGIN      
        1 gctctactac taccatataa ttatatcctt caggaacttc gagttcaaca gttcttacca
       61 ctgtagactc attaatgatc gtgccggttt cggcattttt tttatctgct atcaggttgg
      121 atgtagcctc tcttgccttg acaatcatct tgaggttatc ggaaacatca ggtcctttat
      181 tttcaagata tatgtctgat tttattgata ccttgccagc cgatacgccg cccactgtga
      241 aatcaatatt agtcaggctg accccgcttc gctttgaccc cggtatcagt gcgttcaacc
      301 caaggatatt gaccgtaccg ctgtcccgga tgcttccatt gtcaaaaagc aggatattca
      361 actcgtatcc gccatttctc tccacattga tgttcgttga aacagaaagt gttttgtcta
      421 atcttgaatc tgtttccggt attggagctg aaacctggct ctcaagaagc cttgtttcgc
      481 tgtttatagc ccttattagc attactgcat cccgggtctt gcctccatta tgatttatat
      541 aggctgtgac atttacttct atccgtgcgc ttgtcacctc gaccggcttg acgtcaactt
      601 cccggatgga aaggtaagag ggatatgacg gttctttctg aagaacatct ttatttagct
      661 gtacaatggc aaatgataag atcaaaacgg atgccaatat gattgtaagt atataccccg
      721 gtttcataat tccattttat atactacctc atataatata aaccttatta ttcagcgttc
      781 ctggcagtta attcaggatt tattattcgg agatatcaag ctcaagcaat tcaacaataa
      841 tgggagttcg ttctattttg agattattac attcaacata tagtttttct accatattgc
      901 cagtaaaacc tgcttttaat aattctattc tctcttgcga tgtttctaac atcttaaaca
      961 cctatccatt tatcctatgg tgatcaaatt gtattattaa atatatatag ataccttaaa
     1021 attcttatgc tcagtataat tgtaatgata ataatcataa aaattatgtg tggtattaat
     1081 cctggttgat ggatttagag accattatga atttgaaatt caagaagcgt tctctggaaa
     1141 ggatcttcaa ccacaccttc cagtacttta taatgttttt ttattcgctt catcagattc
     1201 gccctgaaat caggatccgg aaagagatac gcaggatcct taatgacttt catggtattg
     1261 aggtcaaata gtcccagatc ttttatttca aatgttgctc ttgagaagcg tttcgcagcg
     1321 agataaaaat attcacatgt atcacggtat gtttcaaggt atgaaagggc aagtttggaa
     1381 ttgacgaggg cattccattc tttgatttca attcctacaa tatagaatct ttcggattgt
     1441 ttgaagactg ctattatatc tgcaaggttg catacgctct tgcggcaaag gaaaggcttg
     1501 atgtgtatct caggtgtgtg aaagaccggg attccgtgat ttgtaatgga attgactatc
     1561 tcatgagagt tagttgtcat gccaaagtgc gagaacatca tctttttcat gcatggggtt
     1621 ctaaaataaa aacattgtgt gaggggtgtg aaagtgttga ccgtattgat cgatagggat
     1681 tactttcact ccgctcttgg atcgggaata tatattaaac cgttaatctt ccggtgaggt
     1741 gaaaaacatg gatatgttat ggagcggcct tgcaaggcgt gcatctgaat atacaaggat
     1801 tgaaaggaaa agccagttca gtggagtaca tgaaattgcc tttaaatcca cgaaagtgca
     1861 ggatcaaagt aaagatatat agaaaaaaag gtttgatgcc gcaggcatca agggagtgca
     1921 agcgcatgat gcgccgccct cctccttccc aaaaaccttt ttgatctggc tccccttttc
     1981 ttgaacttta ccggctatct tccgtctttg ccttctgttc ccgggtggga gcttcttgct
     2041 gaggttctcg gtgattttct tgatatttct ttattccttc ctttttcgcc acattagcag
     2101 gagcccaaaa atacctgttg atatcagtaa caatgtggga agctcggaaa ctggcgatgg
     2161 tggtacaggt attggagtat gtgttacagt gggttttgg
//
LOCUS       DATGBN010000208         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_443351, whole genome shotgun sequence.
ACCESSION   DATGBN010000208 DATGBN010000000
VERSION     DATGBN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443351"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..530)
                     /gene="cas8a1"
                     /locus_tag="VKL21_04970"
     CDS             complement(63..530)
                     /gene="cas8a1"
                     /locus_tag="VKL21_04970"
                     /inference="COORDINATES: protein motif:HMM:NF021241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HMB45153.1"
                     /translation="MKLDNSIFYQFTGNPFVDAGIWAICGWVDKVKPEELNKEDLKRI
                     AKDITPLYLTSAWSKSIYSIFPNNPITNPSVKYKESRLKEFLNNLIEELEPINDSGNC
                     ISCGRRDVKVIRTKTEIPLIGSGGLINYFPFGQPGADYCPSCTLALVSTDLMG"
     gene            complement(561..785)
                     /locus_tag="VKL21_04975"
     CDS             complement(561..785)
                     /locus_tag="VKL21_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45154.1"
                     /translation="MMVEKLGGKTKDTGMVKFSFSICSRDIGNDNCEALAKMFFLQGA
                     SSKPGLLQISDSRIEHNNTNIKTKKRISKA"
     gene            complement(1124..1438)
                     /locus_tag="VKL21_04980"
     CDS             complement(1124..1438)
                     /locus_tag="VKL21_04980"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB45155.1"
                     /translation="MDKEKIEIVGKLRDFIKKHTVEKAILFGSFARGEQREDSDIDLI
                     LVSKQFEGKSALKRPVPFYIDWHLGYPVDFLCYTPKEFNDLRKQVSIVSQALKEGIEI
                     TG"
     gene            complement(1689..2042)
                     /locus_tag="VKL21_04985"
     CDS             complement(1689..2042)
                     /locus_tag="VKL21_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HMB45156.1"
                     /translation="MPLTKVSHKFQVVIPKEVRESLGISQGDKLEVYEKNHERFSEFY
                     DKAPIDHFDVIETANIKPKRIKIAEGYRRCTLMSLRIQASPELLKFAYEAGLGEKNAM
                     GFGCVDVVGPNQSIR"
     gene            2136..3557
                     /locus_tag="VKL21_04990"
     CDS             2136..3557
                     /locus_tag="VKL21_04990"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMB45157.1"
                     /translation="MKEYDLIVIGTGSGMNYVGSILEKNPDFKVAVVDKDEPGGICLT
                     RGCIPSKLLLYPAELIRTIGTAGKFGIEVTLKNIDFKYIMDRMRSDISKDVDSIRKGL
                     SSDPNLDYYHDTAKFISPYTMKVGNETITSKMIFLCTGSRTIIPKVKGLEETGYLTSD
                     TVLELSMLPPAIAIIGGGYIAAEYGHFFSSMGSRVTIIGRNNLFIPEVEPEISRLAKR
                     EMSAFMDIFTGHEVVEVQKSGDGKKKVIAISEGQKVDFIVDEILVASGRGPTNDILHP
                     EKSGIKTDPKGWFEVNEYLETSKPNVWAFGDCNGKYLFKHVANYESKIVFNNAIMKQK
                     IKVDYHAVPYAVFSYPEIAGVGMGEKEALEKYGAENILIGFHKYEDTAKGHAMEVEDY
                     FVKIIFENNTHKILGAHIIGPYASILIQEIVNLMYTKEESSEPIIQGMHIHPALSEVV
                     QRAFYSLVPQEHYHHLLGHMGLE"
BASE COUNT         1084 a          734 c          699 g         1121 t
ORIGIN      
        1 tatacactca aggtcataat taattattct taatattcaa ttaatctcca cttattaaaa
       61 cattacccca tgaggtcagt ggacactaac gctagggtac aggaaggaca ataatcagcc
      121 cccggttgac caaaagggaa ataattaatt aatccaccag aaccaattaa tggaatttca
      181 gtttttgtcc taatgacttt tacatcgcgc ctgccgcaac taatacagtt tccggaatca
      241 ttaatgggtt caagttcttc tatgagatta tttaggaatt cttttagtcg gctttctttg
      301 tattttactg atgggtttgt tattggattg ttcggaaata ttgagtatat gcttttggac
      361 caagctgagg ttaaatataa cggagttata tctttagcta ttctctttaa atcttcttta
      421 ttcagttctt caggttttac tttgtccacc catccgcata tagcccatat tccagcgtca
      481 acaaatggat ttccagtgaa ctggtaaaag atactattat ctaatttcat tttatctatt
      541 attaattctc agttgataat tcacgcttta gatatccttt ttttagtttt aatatttgta
      601 ttattgtgtt caattctact atcgcttatc tgaagaagcc ccggcttcga gcttgcaccc
      661 tgcaaaaaga acattttcgc aagtgcctcg caattgtcat tccctatgtc tctgctgcat
      721 atagaaaatg aaaatttcac cattccagtg tcttttgttt ttccacctag tttctccacc
      781 atcatagact gtttaaatta gctctgagat tataaacctt tttagagcgg agtgaaacta
      841 ttttttttcg aggatgtcgt tgagcaaaaa gagatttttt aattttttct ggagaaatgg
      901 ttaatcgcaa gtaaaatcaa ccacagagtc cacagagaac acatagttcg ttgacgcttt
      961 tctatgtgga ctctgtgctc tttgtggttt atgcattcag atttcatgca accgatgatc
     1021 ttacagaaaa ctccgccatc ggcggcgcgt ccccgggggt atggggtcgc accccagcgc
     1081 cgtaaagctg aaaaagacaa aactctttaa tggtacgctc atttcaacct gtgatctcta
     1141 ttccttcctt caacgcctgg cttacgatgc tgacctgctt cctgagatca ttgaattctt
     1201 ttggagtata gcacaaaaaa tcaaccggat agcccagatg ccaatctata tagaacggca
     1261 caggtctttt caatgcactt ttcccttcga attgtttgct aaccagaatt aagtcaatat
     1321 cgctatcctc ccgctgttct cctcgtgcaa aagaaccaaa gaggattgcc ttctcaactg
     1381 tatgtttctt tatgaaatct ctaagttttc ctacaatttc aattttttct ttatccattc
     1441 cagtatctcc atcgaagatt tcaaaatttc ttccacttca cctttggcaa aatccgatgc
     1501 cacgtcagga tagcgcgtgg atgtataggc tggatttatc agggcgcata attctgttat
     1561 tttcgaaggc gctttagatc tctattagcc tgtttccacc aattttcagt ttcctttttc
     1621 atcacatctt ctcttttctt taaagaacct tcatctttat aaagataatt agagcaaggc
     1681 gaaactgttc atcttatcga ctggtttggg cctactacat ccacacaccc gaaccccatc
     1741 gcgttcttct caccaagacc agcctcataa gcgaacttca aaagctcagg acttgcctgg
     1801 attcgcaggc tcatgagagt gcatcgcctg tagccttctg ctattttgat gcgctttggc
     1861 ttgatattgg cagtttcaat tacatcgaaa tgatcaatgg gtgctttatc ataaaattcg
     1921 ctgaaacgtt catgattttt ttcataaact tccaatttat caccttgaga gatacccaga
     1981 ctctctctga cctccttcgg aattacgacc tggaatttat gactaacttt ggttaaaggc
     2041 atagttgtac ttcatagttg tactgatatt tctatttaat ctggaaaaag catttataca
     2101 aataaagcaa gtatttccaa taatggggtg tctaattgaa agagtatgac ctgatagtaa
     2161 ttggcacagg ttcgggaatg aactatgttg gctcgatcct tgaaaagaat cctgatttta
     2221 aggtagctgt agtcgataag gacgagcctg gcggaatatg ccttacgcgc ggatgcatac
     2281 cctccaaact tctgctctat cctgcagagc tgatcagaac gatcggaaca gcaggaaaat
     2341 tcgggatcga agtaacattg aaaaatatcg atttcaaata tattatggac aggatgagat
     2401 cagatatttc aaaagatgtt gattccatac gcaaaggact gtccagtgac ccgaatcttg
     2461 actattatca cgatactgcc aaattcatat ccccatatac tatgaaagtt ggcaatgaga
     2521 ccattacttc aaaaatgatc tttctctgta cgggctcaag aactataata cccaaagtta
     2581 agggattgga ggaaacaggt tatctcacaa gcgatacagt actggaatta tccatgcttc
     2641 cgcctgctat tgcaattatc ggtggtggat acatagcggc tgaatatggc catttctttt
     2701 catcaatggg ttccagggta acaattatcg gaaggaataa cctgttcatc cccgaagtgg
     2761 agcctgagat ttccagactc gcaaaaagag agatgtccgc attcatggat attttcacag
     2821 gccatgaagt agtcgaagtc caaaaaagtg gagatggaaa aaagaaggtg attgccataa
     2881 gtgaagggca aaaagttgat tttatcgttg atgagatact ggtcgcctcc gggcgcggac
     2941 ccaccaatga catccttcat cctgagaaaa gcggaataaa aaccgatccg aaaggatggt
     3001 ttgaagtaaa tgagtatctt gaaacttcga agccaaacgt atgggcattt ggtgattgta
     3061 atggtaaata tctcttcaag catgtggcaa attatgaatc aaagatcgtt ttcaataatg
     3121 cgatcatgaa acagaagatc aaagttgatt atcatgcagt cccttatgct gtattttcat
     3181 atcctgaaat agcaggagtt ggcatggggg aaaaagaagc acttgaaaaa tacggggcgg
     3241 aaaacatatt aataggtttt cataaatatg aagatacggc aaaaggccat gccatggagg
     3301 ttgaagatta ttttgtaaag atcatatttg aaaacaacac ccataagata ttaggcgccc
     3361 atatcatagg tccgtatgca tcaatcctta tacaggaaat cgtgaacctg atgtatacga
     3421 aagaagaaag ctcagaacct attattcagg gaatgcatat ccatcccgca ttgagcgaag
     3481 ttgtccagag ggcgttttat tcattggtgc cgcaagaaca ttatcatcat ctgcttgggc
     3541 atatgggact ggaataagat cgatagcgat gattataatt gaaaactaaa gaatatatct
     3601 cctgctcgga gatatcacag aacaacgaac ggatgcga
//
LOCUS       DATGBN010000209         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_443558, whole genome shotgun sequence.
ACCESSION   DATGBN010000209 DATGBN010000000
VERSION     DATGBN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443558"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VKL21_04995"
     CDS             <1..1518
                     /locus_tag="VKL21_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMB45158.1"
                     /translation="LSIVAIVLLLPMLGGTSGFSAVSVLLNVFWALAIVGLVIAVSRF
                     VVPLMLNNIVQTHSRELFLISIVLICIGTAWLTSLVGLSLALGAFLAGLVISESAYGH
                     QAFSEILPLKDIFVILFFVSIGMLLDINSVIRNPFFVLLAVLTILVIKFAAGMLVPIV
                     LGYPIRIGVLVGVALAQIGEFSFVLSKEGIDLGLLTADDYQVFLASAIITMVFTPFMI
                     QGSGRFSEYFERLPVSKALKFGRFHEEIIKKTELKKHVIIVGFGLNGRNVSRVLSASG
                     IPFIVLELNPETVRVEKKRGVPILFGDASKESVLEHANVTVALSVVISISDAAATRRI
                     VELARRLAPSVYIIARTRYTNEVNPLYSLGANDVIPEEFETSIEIISRVLHRYFIPAD
                     EIEKHIREVRKGGYEIFRNVQQKYSLFPELKQHLPDIEIETIRIKETSPLVGKTLAQV
                     GFRNKYGVTVLAIQRDKETITNPSGDTGLMALDIVILIGTRDNISSVSCAVLSTS"
     gene            complement(1547..2029)
                     /locus_tag="VKL21_05000"
     CDS             complement(1547..2029)
                     /locus_tag="VKL21_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMB45159.1"
                     /translation="MRIRKATQEDFLRVHQFTAGCPPMENYPEHVYKIILRYFGDYCF
                     IGEENEQIIGFVLGIVPQGFQGTYFLWEIGVTPTYRGQGIGGKLVREVEKELGKMGFK
                     RIELTIDPVNLPSQKLFKKMGYENISERVGKTIEVQGNRAVQDYYKPGRHFMVFEKLI
                     "
     gene            complement(2026..>3134)
                     /gene="purD"
                     /locus_tag="VKL21_05005"
     CDS             complement(2026..>3134)
                     /gene="purD"
                     /locus_tag="VKL21_05005"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMB45160.1"
                     /translation="SPLSGGLVDELQANCILAFGPVRQAAQLEANKEFSRRLLQKYMP
                     FAIPQFQVFDDPQKAIDHAEELDWQVAVKPIGLTEGLGVRVFGEQLKDENEVKKYIQK
                     IINNRIGGSSKIIIEEKIEGEEFTVQCFVHGEQILPTPAVQDFKKLLPGEKGPNTASM
                     GSYSQKGHLLPFMKEKDYETALEIIRITLKGFQKETGSTCTGFLYGQFMMTSKGVKLI
                     EYNFRPGDPEWINTLIILKNNLLDVILNLFQGEKQALYFENQATVCKYIVPEGYPEKL
                     YQNLDVDLDKEAIQKIEGVRYYYSCGLDNKGKLNVGTERGIAFIAKASTIEEANDKVE
                     KAISLVKGKFYYRKDIGTKDLIDSKIDHVKRLSR"
BASE COUNT          808 a          681 c          629 g         1016 t
ORIGIN      
        1 cttagcattg tggcgatagt tttacttctg ccaatgctgg gcggtacgtc tggtttttct
       61 gcggtcagtg ttttactgaa tgttttctgg gcccttgcta ttgtgggact tgtgattgcg
      121 gtatcccggt ttgttgttcc attaatgctg aacaatattg ttcagacaca cagccgcgag
      181 ttgtttttaa ttagcattgt actgatctgc atcggaaccg catggttaac atcccttgtg
      241 ggtttatcgc tggcacttgg cgcttttcta gcgggtcttg tgatctctga atccgcgtac
      301 gggcaccagg ccttcagtga gatacttcct cttaaggata ttttcgttat tctttttttt
      361 gtgtcaattg gcatgctgct ggatataaat tccgtaattc gcaatccgtt ttttgtactc
      421 ctggcagtgt tgacaattct tgtgatcaaa tttgccgccg ggatgttggt ccccattgta
      481 ctgggttacc ccatcaggat cggcgtactg gttggagtag cgcttgccca gatcggagaa
      541 ttctcatttg tactgtcaaa ggaaggaata gatcttggtt tactgacagc tgatgattat
      601 caggtttttc ttgcttctgc tatcattacg atggtgttca ccccattcat gatccaggga
      661 agtggtaggt tttcagagta tttcgaaaga ttaccggtat ctaaggctct taagttcggg
      721 cgctttcatg aggaaattat caagaaaaca gagcttaaga aacatgtgat tattgttggt
      781 tttggcctga acggcagaaa tgtttcaaga gtactgtctg catccggtat tcctttcata
      841 gttcttgaat tgaatcctga aaccgtacgc gttgagaaaa aacgaggcgt tcccatatta
      901 tttggtgatg cttcaaaaga atcagtgcta gaacatgcga atgtaaccgt ggcgctttcg
      961 gtggtcattt ccatatctga tgcggctgcc acccgaagga tcgtagaact ggctcgaagg
     1021 cttgctcctt cggtttacat tatcgcccgg acccggtata cgaatgaagt caatccgctt
     1081 tattcacttg gcgctaatga tgtaatacct gaagagtttg aaacttcaat tgagatcatt
     1141 tcaagggtat tgcaccgata ttttattcct gctgatgaga tagaaaaaca tatccgtgaa
     1201 gtcagaaagg gaggttatga gatatttcgc aatgtgcagc aaaagtattc tttgtttccg
     1261 gaattaaaac aacatttgcc ggatattgag atcgagacga tcagaattaa ggaaacttcg
     1321 cccctggtgg ggaagaccct ggcccaggtt ggcttcagga ataagtatgg ggtcaccgtg
     1381 ttggcgatcc agcgggacaa agagacaatt accaatcctt ccggagatac cgggttgatg
     1441 gcgctggata ttgttatttt aattggaacc agggataata tttcaagtgt gtcttgcgcg
     1501 gttttgtcaa cttcataaaa gtggactcaa aaaaaataat tcatagtcag ataagtttct
     1561 caaacaccat gaaatgcctt cctggtttgt agtaatcctg cacagccctg ttcccttgaa
     1621 cctctatggt tttccccact ctctcactga tattttcata acccattttc ttaaagagct
     1681 tttgagaagg aaggttaaca gggtctatgg tcaattcaat tcttttaaat cccattttcc
     1741 ctaattcttt ctcaacttcc ctgaccagtt ttcctcctat accctgtccc cgataagtgg
     1801 gagttacacc aatttcccat aaaaaatatg tgccctggaa accctgtgga acgattccca
     1861 gaacaaaccc tattatttgc tcgttttctt cgccaataaa gcaataatcc ccgaagtaac
     1921 gcaaaatgat tttataaaca tgctccggat agttctccat gggcggacat cccgcagtaa
     1981 attgatgtac tcttaagaag tcctcctggg ttgcttttct tatcctcatc ggctcaacct
     2041 tttaacatgg tctatctttg aatcgattaa atccttcgtt ccgatgtctt tcctgtaata
     2101 aaattttccc ttcaccaggg aaatggcttt ttccacttta tcattagctt cttcaattgt
     2161 ggacgcttta gcgataaaag caattcccct ctcggtcccc acattcaatt tacctttatt
     2221 atctaagcca cagctgtagt aataccgaac accttctatc ttttgaatgg cttccttatc
     2281 taaatccaca tccaggttct ggtagagttt ttcaggataa ccttcaggca ctatgtattt
     2341 acagactgtt gcctgattct caaaataaag cgcctgtttt tccccttgaa atagattaag
     2401 aatgacatcc agaagattat ttttcaatat gattagggta tttatccatt caggatctcc
     2461 gggtctaaaa ttatattcaa ttaatttgac cccctttgaa gtcatcatga actgaccata
     2521 taaaaaacct gtacaggtac ttccggtctc tttctgaaaa ccttttaaag taatgcgaat
     2581 aatttccaga gccgtttcat agtctttctc tttcatgaaa ggaagaagat gtcccttttg
     2641 agaatatgag cccatacttg ccgtattcgg tcctttttcc ccgggcaata acttcttgaa
     2701 atcctggaca gcaggagtag ggagaatttg ttccccatgg acaaaacatt gaactgtgaa
     2761 ttcctccccc tcaatttttt cttcaataat tatctttgaa cttcctccaa ttctattgtt
     2821 aattatcttc tgaatatatt tcttgacttc gttctcatct tttagctgct ctccaaaaac
     2881 cctgacccca agtccttctg ttagcccgat cggttttacc gcaacctgcc agtccagttc
     2941 ttcagcatga tcaatagctt tttgtggatc atcaaaaacc tggaactgag gaatagcaaa
     3001 aggcatatat ttttgaagaa gtcttctgga aaattcctta tttgcttcca actgggcagc
     3061 ctggcgaaca ggtccgaagg ctagaataca atttgcctgt aattcatcaa caagacctcc
     3121 ggaaagaggg gaag
//
LOCUS       DATGBN010000210         8106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_521208, whole genome shotgun sequence.
ACCESSION   DATGBN010000210 DATGBN010000000
VERSION     DATGBN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521208"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VKL21_05010"
     CDS             <1..755
                     /locus_tag="VKL21_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMB45161.1"
                     /translation="EAFRRNTKLAFFESPANPTMKLTDIKAVSDMSHDRGAAVMVDNT
                     FMTPYFQKPLLLGADVVVHSATKYLNGHGDTLGGIIVGSSDYIKSARHTAKNIGGSIT
                     PFSAWLIIRGIKTLSIRMDRHNENAMAVAEFLQGHDKVETVIYPGLPGHPQYGLGKKQ
                     SSGAGGIISFLLKDAKDVPVFLDSLKLCTLAVSLGETATLIQHPATMTHAVVPREDRV
                     KYGIMDELIRLSVGLEDLSDIIDDLAQGLDNI"
     gene            complement(765..902)
                     /locus_tag="VKL21_05015"
     CDS             complement(765..902)
                     /locus_tag="VKL21_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45162.1"
                     /translation="MNILEVTEDKIFQTLGKGRDPKYKPIDKTKLIKTNEPFDYTSLV
                     K"
     gene            1025..1852
                     /locus_tag="VKL21_05020"
     CDS             1025..1852
                     /locus_tag="VKL21_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HMB45163.1"
                     /translation="MVVKVGFIKLGNLGTSQVIDLLLDEIAAREGIAVRAFGTGAKMG
                     KDEAAETASFKNWGPDFVVMISPNSSAPGPTAARDVWKGTPTIVVSDGPTKKEDREKL
                     EQAGFGYMILPVDPLIGAKREFLDCVEMASFNTDALKVLSVCGAIRLVQTEIDKVIDQ
                     VAAGTKPLVLPKILAKPDKCVESAGFNNPYAKAKALAALHIATKVAEIDFPACFVLKE
                     ADQVATTAAAGHEAMRAASRLADEAREMEKANDSVLRKPHAKDGRLLNKTKLLEKPQ"
     gene            complement(1994..2293)
                     /locus_tag="VKL21_05025"
     CDS             complement(1994..2293)
                     /locus_tag="VKL21_05025"
                     /inference="COORDINATES: protein motif:HMM:NF036315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5371 family protein"
                     /protein_id="HMB45164.1"
                     /translation="MKLITAIKMIEEREKRQRKTEKEMRRNGIFLKNNSTDKENSSSN
                     LKEEQKEQKNLVHVQSIFPQDDIVALKVKTREAHVREAISKAVYFYLKSANEDRT"
     gene            complement(2501..2809)
                     /locus_tag="VKL21_05030"
     CDS             complement(2501..2809)
                     /locus_tag="VKL21_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HMB45165.1"
                     /translation="MSSEMCAVCGLPKELCICEEVAKEQQRIVVKIDTRKYKKEVTVI
                     DGLNPNEINLQELTSYLKSKFACGGTIKDNVIELQGNHKSRMKEVLIKKGFLPEQIKI
                     "
     gene            2866..3222
                     /locus_tag="VKL21_05035"
     CDS             2866..3222
                     /locus_tag="VKL21_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HMB45166.1"
                     /translation="MLPVLKYNGVVIAKNIEFARTMLTQSLGLMFRKSMLHNYSMIFI
                     LKKPYRVNIHMLFVFFRIDVIFLNEDKKVMGFSQLKPWVGYKGMEDIKYILEMKAGTI
                     DMFDLKVGGQMEFDES"
     gene            3249..4304
                     /gene="endA"
                     /locus_tag="VKL21_05040"
     CDS             3249..4304
                     /gene="endA"
                     /locus_tag="VKL21_05040"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HMB45167.1"
                     /translation="MALKGKLSIDSVLLEKQAIEELHNKSYYGRPKGDILEISLPESA
                     FLIYMEKIRVEFEGKEIGFEDFFLKASSILKNFELLYIVYKDLRERGYYVQPGVTGFR
                     VYPRGGHPGKTPAEFFVFVTSERIPLLLSQLRLHLETVGNLKKRLVVGIVDEDSDITY
                     YEVKKTSPSGTYEPGLKKGLSKASLLEDRVMVWDKDASLVLQRDGFFGKPMDEGHLQL
                     SLIESCYLLNKEILEIENRNHMLLDFRMFTKTASNIESDFMIKYAVYEKLRNNGLVPK
                     TGFKFGTHFRVYKKIDDMKKLPHSDYLVHAVEQGHIFSLQQLSRAVRLANSVRKEMIY
                     GTVDSQVDFFIIGRMRL"
     gene            complement(4350..4556)
                     /locus_tag="VKL21_05045"
     CDS             complement(4350..4556)
                     /locus_tag="VKL21_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5371 family protein"
                     /protein_id="HMB45168.1"
                     /translation="MAKIMHAQTVLTEEDIETLKLKTGETSTKDAIAKAVSHYLECEY
                     TKVEDMWAKKLEKVVKRKQKEEEE"
     gene            complement(4669..5673)
                     /locus_tag="VKL21_05050"
     CDS             complement(4669..5673)
                     /locus_tag="VKL21_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45169.1"
                     /translation="MKKIIVCFIIVLLMVFPASAGEVSLNKWVLNVTIGDDGMVEETI
                     QTEFENTGTTSLDGFSFVVPASRVVISSDNIMSIPSTGTEVKQQTIPGGIKISINFDR
                     PVEAGKKWNGRLSLTAENWAVKEGPDYSITIPIDAPKAIMAGKEVEFSIPADPEIRSQ
                     VFLPKAVNVTAVEVKSRDKKSYKKLLQFDKMVITWFQLSFGDAFSIKGSYSDILKLII
                     ETDEKSRQIKDKIKKARDAGQDVKEAESHLLNAEEYNNNQALQSFWINDFNVVQEYVG
                     YANDELSLAEKSLSEPKETKVIPEETKESNRIPGFGGAGLIIIISSYCMILRRKRTKS
                     "
     gene            complement(5705..7618)
                     /locus_tag="VKL21_05055"
     CDS             complement(5705..7618)
                     /locus_tag="VKL21_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HMB45170.1"
                     /translation="MRKEFRELVSRDKAKKIIDSLDIKSHPEEVDIENARDHVLAADV
                     ISQVDVPPFTRASMDGYAVHASDTYNAREDRPVRLKLAGSILVGINPGIFLERGQAAE
                     IATGAVMPEGADSVVMVEYTHIDNDIIVHRPVSINENVMQAGSDIMVGERVLRRGTCL
                     GAREIGVLCAIGKSRIRVLGLTVGIISTGNELKEPGCQLDEGEIYDVNSFSLSCAVKE
                     CGGTPIRYGNVNDNENEIKKALIELSKCDLILTSGSTSAGSGDMMYRIIEENGTVLAH
                     GIDIKPGKPAIIGQVYGKPIFGLPGYPASSLTIFNEFVAPLIRKMTGKTSTNVKTHAR
                     TAIRIRSDGRSQLLPVGVVRGMAYPIEKGSGAITTLSEADGFIEIPSSIEMIEAGEDV
                     EVTLYGELYPVDLFFVGSQCLGLDTIADMTGIKMRIINVGSSGGLSAVKNGIADIAGV
                     HLLDESGVYNIPFLSRFGLYDAVLVKGYLRDQGLIIPKDSKIKEFADLVNYRFINRNT
                     GSGTRVMTDLMLKKIANEREISFEEFVNGIKGYHTEAKTHSAVAAAVKLGKADVGIGI
                     RTVAELNGLKFIKIADEEYDFVIPERLMEKDEILKFQDVLMSREFGDNLPPGLRVYGR
                     TGEVVHLKEGETL"
     gene            7735..>8106
                     /locus_tag="VKL21_05060"
     CDS             7735..>8106
                     /locus_tag="VKL21_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB45171.1"
                     /translation="MLYAIETSGITKRFEEVTAVNNISISVRPGELFGLLGPNGAGKT
                     TLINMLSTMIEPSGGSALVWDHDVVKEADLVRQNIGVVFQDTTLDDRLTGRENLDLHG
                     RLYGLDKKTRKDRIQDVLSLVE"
BASE COUNT         2316 a         1730 c         1553 g         2507 t
ORIGIN      
        1 aggaagcttt ccgcaggaac acaaaacttg cattctttga atccccggcc aatccaacca
       61 tgaaactcac agatataaaa gctgtaagcg atatgtcgca tgacagagga gcagctgtaa
      121 tggttgataa taccttcatg accccatatt tccagaagcc tcttttactt ggagctgatg
      181 tggtagtgca cagcgccacg aaatacctta acggccatgg ggatactctt ggggggataa
      241 ttgtaggttc ttcggattac ataaaatcgg caaggcacac ggcaaagaac atcggcggct
      301 ccatcacacc gttctcagca tggctcatta taaggggaat aaagaccctt tctatccgta
      361 tggacaggca taatgagaat gcaatggcgg ttgcagaatt cctgcagggg catgacaaag
      421 ttgaaactgt aatttatccg ggccttcccg gccatccaca gtatgggctt gggaaaaaac
      481 agagcagcgg cgccgggggt ataatctcct tcctcctgaa agacgctaaa gatgtgcctg
      541 tatttcttga ttccctgaag ttgtgtactc ttgctgtcag ccttggtgag actgcaacgc
      601 ttatccagca tcctgcaact atgacccatg ccgttgtccc gcgggaagac agggtaaaat
      661 acgggattat ggatgagctt atcaggcttt ctgtgggact tgaggatcta agtgatatta
      721 tcgatgatct ggcacagggg cttgataata tttaagaaag gattttattt taccagggaa
      781 gtataatcaa aaggttcatt ggtttttatt agtttggttt tgtctatggg tttatatttc
      841 ggatctctcc ctttacctaa tgtttggaat attttatctt ccgtaacttc aagaatattc
      901 atcagttcct ccgcccactt caataacctt attatttata aaattatgct ttattaattt
      961 agaattccag aacaatttca aaacccttat aaacgaaccc agcgaaattc tactgtgatc
     1021 aaatatggtc gttaaagtag gttttattaa acttggaaat cttggaacct cacaggtaat
     1081 agatttatta cttgatgaga tagcagcgag agagggaata gcagtccgtg cattcggaac
     1141 aggggctaag atgggaaagg atgaggccgc agaaacggca tccttcaaga attgggggcc
     1201 ggactttgtt gttatgataa gccctaattc cagtgcgccc ggcccgacag cagcacgtga
     1261 cgtctggaaa ggtactccaa ctatagtagt atcagacggc cccacaaaga aagaggaccg
     1321 ggaaaaattg gaacaggcag gttttggtta tatgatcctt cctgttgacc cgttgatcgg
     1381 cgcaaaaagg gaattccttg actgtgttga gatggcgtcc ttcaatacgg atgcattaaa
     1441 ggtattatcc gtatgcggag ccataaggct tgtccaaact gaaattgata aggtaatcga
     1501 tcaggtcgca gcaggtacaa aaccacttgt actccccaag atccttgcaa aacctgataa
     1561 atgcgtggag agcgcaggat tcaataatcc ttacgcaaag gcaaaggccc ttgcagcatt
     1621 gcacatagcc accaaggtcg cagagataga cttccccgca tgctttgtat taaaagaggc
     1681 cgatcaggtc gctacaacag cagcagcagg tcacgaagct atgcgcgcag catcaagact
     1741 tgccgatgaa gctcgcgaaa tggagaaagc aaatgactca gtcctcagga agccgcatgc
     1801 taaggatgga aggttgttga ataagactaa actcctggag aaaccacagt aaagtaatcc
     1861 ttcaaggaat cttattatcg tcaagaacat aattctgcaa agatggtttg atttgtctgg
     1921 ttcttgatga taacttcctc tttatctttt tttttcaaac ataaaaaata aatccccggc
     1981 acaaaaaccg ggattaagtt ctatcctcat tggcgctttt caaatagaag tatactgctt
     2041 ttgaaatcgc ttcccttaca tgcgcttctc ttgttttcac tttcaatgca acaatatcat
     2101 cctggggaaa aatggattgt acatgaacca ggtttttttg ctctttttgt tcttctttta
     2161 aattgctgct tgagttttct ttatcagtgg aattattttt caaaaaaatt ccatttcttc
     2221 gcatctcttt ttctgtcttt ctttgtcgtt tctcacgttc ttcaatcatt tttattgcgg
     2281 ttatgagctt catatttttc aactcaattt gttaatatca ttatgattag tataataatg
     2341 tattaacgta aaaagatttc cctcaatttc aagaccaaag taaaattatc ctaaagctgc
     2401 caaatttagg taataaaatt agataatcac tgcttaaaac aactggagtt acggtttaaa
     2461 ataagagaga tactattaaa cccaccccac catgaacaat ttatatcttg atttgctcag
     2521 gtaaaaatcc tttcttaata agaacttcct tcattctgct tttatgattt ccctgaagtt
     2581 ctataacatt atcttttata gtaccaccgc atgcgaattt tgatttaaga taagatgtca
     2641 attcctgcaa atttatctcg tttggattaa gaccatcgat cactgttact tctttcttat
     2701 atttgcgggt gtctatcttt actactattc tctgttgttc ttttgctact tcttcgcata
     2761 tgcaaagctc ttttggcaaa ccgcatacag cacacatttc tgaactcatt tattgggtta
     2821 atacctccat tcttatatta cacaagattg ttatatagct acttgatgct tcccgtatta
     2881 aaatataatg gtgtggtgat agcgaaaaac attgagtttg ctcggacaat gttgacacaa
     2941 tcgctgggtt tgatgttcag gaagagtatg cttcacaatt attcgatgat atttattttg
     3001 aaaaaaccat accgggtcaa tattcacatg ctttttgtat tttttcggat cgatgtaata
     3061 tttttgaatg aagacaagaa agtaatgggt ttttcccaat taaaaccctg ggttggatat
     3121 aaggggatgg aggacatcaa gtacatcctt gaaatgaaag caggaactat cgatatgttc
     3181 gatcttaaag tcggggggca aatggaattt gatgaaagct aaaataaatg gaacactaat
     3241 ataaaattat ggcactcaaa ggaaaattat caatagattc ggtattactt gaaaaacagg
     3301 cgattgagga attgcataat aaaagttatt atggtcgccc taaaggcgac atcctcgaaa
     3361 tttcccttcc tgaaagcgcc tttcttattt acatggaaaa aatacgagtt gaatttgaag
     3421 gaaaagagat tggttttgaa gacttcttcc ttaaggcttc atccatatta aaaaattttg
     3481 agttattata tatcgtatat aaagatctaa gagaacgtgg atattacgtt cagccaggag
     3541 tcactggatt tcgcgtttat cccaggggtg gacatcccgg aaaaacgcct gctgaatttt
     3601 ttgtatttgt tacatcagaa cggataccac tattgctttc ccaattgaga ctgcatcttg
     3661 aaacagtcgg gaacctcaaa aaacgtttag ttgttgggat agttgacgaa gatagtgata
     3721 ttacatatta tgaagtgaaa aaaacctctc catccggtac ttacgaacca ggattaaaga
     3781 aagggttatc aaaggcaagc ctgcttgaag acagggttat ggtctgggac aaggatgctt
     3841 ctttagtact ccagagagac ggattttttg gtaaaccaat ggatgaagga catttacaat
     3901 tatcccttat agaatcatgt tatctcttga acaaagaaat actggaaatt gaaaacagga
     3961 accacatgtt gctggatttt agaatgttta caaaaacagc ctcaaatata gaatccgatt
     4021 tcatgataaa atatgctgtt tatgaaaaat tgagaaataa cgggcttgtt ccaaagaccg
     4081 gattcaaatt cgggacgcac tttcgggttt ataagaaaat agatgatatg aaaaaactgc
     4141 cgcactccga ttatcttgtc catgcggtgg aacagggcca tatcttttcg ctgcaacagc
     4201 tttcgcgcgc cgtaagactt gcaaacagcg taagaaaaga aatgatatat ggcacagtcg
     4261 attcgcaggt tgattttttt atcataggac gaatgcgact gtgaaaaagg catgaggctt
     4321 tccctacccc ttgcccccgc gtttcgaaat tattcttctt cttccttctg ttttctttta
     4381 acaactttct cgagtttctt agcccacata tcttctactt ttgtatattc acactcaaga
     4441 taatgagaaa ctgcctttgc gattgcatcc tttgtgcttg tttcgcccgt ttttagctta
     4501 agggtttcga tatcttcttc tgtaagaact gtttgtgcgt gcattatttt tgccatttta
     4561 ttactccttg aagaaaaagc aataccacaa catatacggc atacttttca ttactacctc
     4621 atcatcatag gaaagtattt aaacattact gagataaatt aaaaaatact aggatttagt
     4681 cctttttctt cttaatatca tgcaataact acttattatt atgatcaggc ctgctcctcc
     4741 aaaaccggga attctattac tttctttggt ctcttcagga atgactttag tttctttcgg
     4801 ctcagaaaga cttttttccg caaggctcaa ttcgtcattt gcatatccaa catattcctg
     4861 cacaacattg aaatcattta tccagaaaga ttgaagcgcc tgattattgt tatattcttc
     4921 ggcattcaag aggtgagatt ctgcttcttt cacatcctgc cccgcgtccc tggccttttt
     4981 tatcttatcc tttatttgtc ttgatttttc atctgtttcg attattaact tgagaatatc
     5041 tgaatagctt ccttttatac tgaacgcatc accgaaactc aactggaacc atgtaattac
     5101 cattttatcg aattgtaata actttttata tgatttcttg tccctggact ttacttcgac
     5161 agcagtcaca ttaacagctt tgggaaggaa tacctggctt cttatttcag gatcggcagg
     5221 tattgaaaat tcaacttcct tcccggccat aattgctttc ggggcatcta tcggtattgt
     5281 tatggaataa tccggacctt ccttcacagc ccaattttcc gcagtaaggc ttaaacgccc
     5341 attccatttc tttcctgctt caaccggtct gtcaaaattg atactgatct taattccgcc
     5401 cggtattgtt tgttgtttaa cttcagttcc tgttgaagga atgctcatta tattatcgct
     5461 actgattaca accctggatg cagggactac aaaagaaaaa ccatcaagag aggttgtacc
     5521 cgtattttca aattcagtct gtatagtttc ttctaccata ccatcatccc caattgttac
     5581 atttaatacc catttgttga gcgaaacttc acctgcagaa gctggaaaaa ccataagcag
     5641 gacaataatg aaacaaacaa ttattttttt cataaaactc cggagttatc tggactttta
     5701 aaacttataa agtttccccc tctttgaggt gcacgacttc tccggtcctt ccgtataccc
     5761 gaagccctgg aggcagattg tccccgaatt ctcttgacat gagcacatcc tggaatttca
     5821 atatttcatc tttttccata agtctttcag ggattacaaa atcatattcc tcatctgcta
     5881 ttttaatgaa ttttaaccca ttcagttctg caactgtgcg aattcctatt cccacatccg
     5941 ctttccccag ctttactgcg gcagcgactg cactatgggt ttttgcttcg gtatgatatc
     6001 ccttaatgcc attgacgaat tcttcgaaag aaatttcacg ttcatttgct atcttcttta
     6061 acatcagatc ggtcataact cttgttcccg aacctgtatt ccggttaatg aacctgtaat
     6121 tgaccagatc cgcaaattcc tttattttac tatcttttgg tattatgagg ccctggtctc
     6181 tcaagtatcc ctttacaaga acagcatcat aaagcccgaa ccttgaaaga aacggtatat
     6241 tgtacacacc tgattcatca aggagatgta ctccggcaat atcggcaatg ccatttttga
     6301 cagcgcttaa cccgcctgat gaacccacgt ttattattcg cattttgatg cctgtcatgt
     6361 cagcgatcgt atcaagaccc agacactggc tcccaacaaa gaacagatcc accggataaa
     6421 gttccccata caatgtaact tcaacatctt cccctgcttc tatcatctca attgatgatg
     6481 ggatttcaat aaaaccgtct gcctcggaaa gagttgttat ggctcccgat cccttctcta
     6541 tgggataagc catgcccctg acgaccccaa caggaagaag ctggcttctg ccatcggaac
     6601 gtattcttat agctgttctg gcatgtgttt taacatttgt gcttgtcttt cctgtcattt
     6661 tccttatcaa tggagccaca aattcattga aaatcgtaag agatgatgcc ggatatcccg
     6721 gaagaccaaa aattggtttt ccataaacct gcccgataat tgcaggtttt cctggtttta
     6781 tatcgatccc atgggcaagg acagtgccgt tctcttcaat tatcctgtac atcatatcgc
     6841 cagaaccagc tgaagtgctt cctgaagtta gaatgagatc gcattttgag agttcgatca
     6901 atgctttttt tatctcgttc tcattatcat ttacatttcc gtaacggatg ggagtgcccc
     6961 cgcactcctt tactgcgcat gagagagaaa aagaattcac gtcatagatt tcaccctcat
     7021 caagctggca gcctggctcc tttagctcat ttccggttga gattataccc acggtaagac
     7081 ccaggactct tattctggat ttaccgatcg cgcaaaggac acctatctcc cgcgcgccaa
     7141 gacatgtccc tcttcttaaa accctctctc cgaccattat atccgagcct gcttgcatta
     7201 cattttcatt aatggaaacg ggacgatgga caataatatc attatcaata tgcgtatatt
     7261 caaccatcac tacagaatcc gccccttccg gcattaccgc ccctgttgcg atttctgcgg
     7321 cctgtcctct ttccaggaat atccccggat ttattccgac caggatcgat ccggcaagtt
     7381 tgagcctgac cggcctgtcc tctcgtgcat tgtaagtatc agaagcatga acggcataac
     7441 catccatcga cgccctggta aaaggaggaa cgtctacctg tgaaataaca tccgctgcaa
     7501 gaacatgatc ccttgcgttt tcaatatcaa cctcctcagg atgggatttg atgtccaggg
     7561 aatcgataat tttttttgcc ttgtccctgg aaacaagttc cctgaattcc tttctcatgt
     7621 attcttcatg aattcatctg gttatgaatt tatcttatca acagaaagct tttgtatccc
     7681 tggacccagg gatatatagc aaattctatt atcattcaaa ttaatcatac cgctatgctc
     7741 tatgccattg aaacatcagg aataacaaag cgctttgaag aggtgactgc tgtaaataat
     7801 atcagcatct ctgtaagacc gggggaatta ttcgggctcc ttggtcccaa cggtgccggg
     7861 aaaaccacat tgataaatat gctttcaacg atgatagaac catcaggggg aagcgccctg
     7921 gtatgggatc acgatgtagt aaaagaagca gacctggtga ggcagaatat cggggtggtt
     7981 ttccaggata ccactcttga tgacaggctt accggaaggg agaaccttga cctgcatgga
     8041 aggctctatg gtcttgataa aaagacaagg aaggaccgaa ttcaggatgt gctctctctt
     8101 gtagag
//
LOCUS       DATGBN010000211         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_133292, whole genome shotgun sequence.
ACCESSION   DATGBN010000211 DATGBN010000000
VERSION     DATGBN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133292"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /gene="terL"
                     /locus_tag="VKL21_05065"
     CDS             <1..1450
                     /gene="terL"
                     /locus_tag="VKL21_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HMB45172.1"
                     /translation="QNQKDARVHFIPDLKDRVFVTLRIPEVIKRLIVSCPPRFGKSEV
                     VSRRLPAYVLGKNPDASIIACSYSADLAARMNRDVQRIIDSPEYADLFPESRLNESNI
                     RTVSQGTYLRNSDLFEIVGHSGVYRSCGIGGGITGMGFNSIGIIDDPIKNREEAESPT
                     IRNKIWEWYTDTFYTRRENDAPILVTQTRWHEDDIIGRLLKSSKNEDADQWEVVSLPA
                     MSEEERPAYDIRTGPNQALWPGRYDVERLNQIRATVPTYTWLSMYQQRPSSASGNLFK
                     RENFQYFTDAPLTYDLHKPEGIDKIPKVHCIIFQTCDPAGTAKTSSDFFVLSTWALTK
                     KGELLLLEVFKTKLEGPDHVDFVKAQYQKWHPVAIGFESVSIGKTTFQNLVRERLPVI
                     DLEPKGDKFSRALSAAIRLGNKTTYFLHGAHWLNDFETELLHFPNVKHDDQVDTLSQA
                     DYMLTDIKIQNRFACSVDNFHLSSIATTRFRG"
     gene            1611..2492
                     /locus_tag="VKL21_05070"
     CDS             1611..2492
                     /locus_tag="VKL21_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45173.1"
                     /translation="MPLTITVIGMAHMIISSMFIEPGGKDCYGHYCQPLYHYKMKCDN
                     CGTEFETFNYRKKYCCENCTNQAGIKNRKEKKSQARKRKCQVCGELFIPPRNDAKFCS
                     DKCKQSAYRERKKESKPFLLEFYRLSPELCPDETGKTGFFNSSLYKNEIGLERLIEEW
                     KESSEINNNVDIKLYQNGENSSKQSLYKEYKLETRIDLEYPFLVLLENDKIVYIRELD
                     PHDGLTRNLAFTIICESIEYRDKAKKFGLLGVEYSEALDQFARWKEEKEKAEKKKASI
                     EKAKVTREANILKKQMI"
BASE COUNT          905 a          461 c          535 g          726 t
ORIGIN      
        1 acaaaaccaa aaagatgcga gggttcactt catccccgac ctaaaggaca gggtattcgt
       61 gaccctccgc attcccgaag taataaaaag actaattgta tcctgcccgc ctagatttgg
      121 taaatctgaa gttgtatcaa gacggttgcc tgcttatgtt cttggtaaaa atccagatgc
      181 ctcaattata gcatgttctt atagcgcaga tcttgccgca agaatgaatc gtgatgtaca
      241 acgtatcatc gatagtccag aatacgcgga cctattccct gaatcacgct taaatgaatc
      301 aaatatcagg acagtctctc aaggaacgta tttgcgaaat tcagatcttt ttgaaatcgt
      361 tggacattcc ggtgtttatc gctcctgtgg aattgggggc ggtataactg ggatgggttt
      421 taattcgatt ggtattatcg acgatcccat caaaaaccgt gaagaagcag agagtccgac
      481 gatcagaaac aagatctggg aatggtatac tgacacgttc tacacaaggc gtgagaacga
      541 tgcacctata cttgtgacac aaactcgatg gcacgaagac gacataattg gtaggcttct
      601 taagagctct aaaaatgaag atgctgatca atgggaagta gtgagcttgc cggctatgtc
      661 agaagaagaa aggcccgctt atgacatccg aacaggaccc aatcaagcac tatggccagg
      721 taggtatgat gtggaaaggt taaatcaaat aagggctact gtacctacat acacctggct
      781 ttctatgtat caacagagac cttcatcagc atccggtaat cttttcaaaa gagagaattt
      841 tcagtacttc acagatgccc cgttaactta tgatttacat aagcccgaag gaattgataa
      901 gattcctaaa gtccactgca taatctttca aacatgtgat ccggcaggca ctgcgaaaac
      961 aagttcagat ttctttgtgc tgtcaacctg ggcactgaca aaaaaaggtg aattgcttct
     1021 acttgaagtg tttaaaacca aacttgaagg acctgaccac gtcgattttg taaaagcaca
     1081 ataccagaaa tggcatcctg tagcaattgg ttttgaatct gtttcaattg gcaaaactac
     1141 gtttcagaat ttagtgcgtg aacgattacc tgtgattgac ttggaaccga aaggcgataa
     1201 gttttcgcgg gcattgtcag ccgcaatcag attaggaaac aagacgactt actttctaca
     1261 tggggctcat tggctcaatg attttgaaac cgaacttttg cactttccaa acgtcaaaca
     1321 tgacgatcag gtcgacacac tttcgcaagc agattatatg ttgactgaca taaaaataca
     1381 aaacaggttt gcgtgttcgg tcgacaactt ccatctgtca agcatagcca caactcgatt
     1441 tagaggttaa gagtttgtat taccagttta aaaaagcgct tagaatggct tagattatga
     1501 gtttgtaaag tgttacggat atttcaatag ctcgtagtga gtcctcttat tatccgcaac
     1561 acatatatac agacatcacg tggtgttacg tatattaaga ggtataccag ttgcctctta
     1621 caataaccgt aataggaatg gcacatatga taattagctc aatgtttatt gaacctgggg
     1681 gaaaagattg ttatggacat tattgtcagc cgttgtatca ttataaaatg aaatgtgata
     1741 actgcggtac tgaatttgag actttcaatt atagaaagaa gtattgctgc gaaaactgca
     1801 ctaatcaggc agggataaag aatagaaaag agaaaaagtc acaggcaaga aaaagaaaat
     1861 gtcaggtatg cggtgaatta ttcattcctc ccagaaatga tgcgaagttc tgttccgata
     1921 aatgtaaaca atcagcgtat agggaaagaa aaaaagaatc aaagcctttt ctcttagagt
     1981 tttacagatt atcacctgaa ttatgtccag atgaaactgg taaaacagga ttctttaact
     2041 cttctttata caagaatgag ataggtttgg agcgattaat agaagagtgg aaagaatcat
     2101 ctgaaattaa taacaatgtt gatataaaat tataccagaa tggtgaaaac tcctctaaac
     2161 aatctttata taaagaatat aaattggaaa cccgaataga tttagaatat ccgtttctcg
     2221 tgcttctcga aaacgataaa attgtttata ttagagaact tgaccctcat gatggcctaa
     2281 cgagaaatct tgcttttaca atcatttgtg aaagtataga atatagagac aaagctaaaa
     2341 aatttggttt attgggagta gagtatagcg aagcgttaga tcaatttgct agatggaaag
     2401 aggaaaaaga aaaagcagag aagaaaaaag cttcaatcga aaaggctaag gttacaagag
     2461 aggcaaacat attgaaaaag caaatgattt aattttttat attattgttt agtaccatta
     2521 atacttattt tcaagcctaa tatattgtaa aagaaaccgt atggattcaa tccatacata
     2581 catacacttc taagaaaatc aatgaagggg atagtgcatt taactca
//
LOCUS       DATGBN010000212         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_287826, whole genome shotgun sequence.
ACCESSION   DATGBN010000212 DATGBN010000000
VERSION     DATGBN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287826"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..616)
                     /locus_tag="VKL21_05075"
                     /pseudo
     CDS             complement(1..616)
                     /locus_tag="VKL21_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(754..2892)
                     /gene="acnA"
                     /locus_tag="VKL21_05080"
     CDS             complement(754..2892)
                     /gene="acnA"
                     /locus_tag="VKL21_05080"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HMB45174.1"
                     /translation="MHKSSNTGCTTMINGMGVLGWGVGGIEAEAVMLGQPYHMPVPDV
                     VGFKLSGEIKEKVTSTDLVLTITQILREHNVVGKFVEFYGPGLSKLSLPDRATIANMA
                     PEYGANAGFFPVGAETIKYLLGTGRKKEDVDLIKKYTQEQGLFHTDESPEPLFSETLE
                     LDMSTVEPSLAGPKRPQDRIPLDKLEESFHKAMEETFRRKKKDVEADHEYHRWLGEGG
                     SFSDEHKRIGLMQVEEKYEMKVPIKSGSIVIAAITSCTNTSNPTVMIEAGLLAKKAVE
                     RGLKVKPYVKTSLAPGSLVVTEYLKKSGLMPYLEALGFHLVGYGCTTCIGNSGPIQDM
                     VAKEVKEKNLIVAAVLSGNRNFEGRINPLVKANYLVSPPLVVAYAISGTIDINLKTEP
                     IGFDPNNNPVYLKDIWPDSEDIRHIIENTIDPMLYEKQYSQISHGSTLWEELIVPTGK
                     LYEWEPTSTYIQEPPFFMDFPVVPPEKGDISGARVLAYLGNSITTDHISPAGEFSEDS
                     LAGQYLISKGLSPENFNSYGSRRGNHEVMMRGTFANINLKNMLIPEKEGGWTRYFPKG
                     EEMTIYDAAMLYKQKQTPLIIIAGKEYGTGSSRDWAAKGTYLLGVIAVIAESFERIHR
                     SNLLGMGVLPLQFEKGENADTLGLKGDEVFDILGISNIFPGEELHVVAKADGKETKFK
                     VISRLDAPIEVEFYRNGGILHTFLRNILKK"
     gene            complement(2856..3236)
                     /locus_tag="VKL21_05085"
     CDS             complement(2856..3236)
                     /locus_tag="VKL21_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HMB45175.1"
                     /translation="MSIDFSKIKDVIDTSKGPVVIYRLNRLEEMGFENVSRLPYSIKI
                     LLESLLRQMDGKLITEEDVKSCAQYYKKKRNEHEIPFIPARVLLQDFTGVPLVADLAA
                     MRSEVLRLGGDPACINPVIPAVPR"
     gene            complement(3247..3420)
                     /locus_tag="VKL21_05090"
     CDS             complement(3247..3420)
                     /locus_tag="VKL21_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45176.1"
                     /translation="MEIIDISSILFAYIGVAAVPGTSFYHSEPGKHKLRFTFSKKDET
                     LQEAARRLEKLNK"
     gene            complement(3728..>4134)
                     /locus_tag="VKL21_05095"
     CDS             complement(3728..>4134)
                     /locus_tag="VKL21_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45177.1"
                     /translation="ARKAVYAAHEAGLSTGAFFILCYPGDTDDTVLETLRFAVSLPLD
                     YLSFTMPYPLPGTALYERVNGRISKEWEVTASLISDHVLIFDADFSENKMKFAILKGE
                     AQFEMKKRFGNYSFLVMKPFDLLTNFMFRLMK"
BASE COUNT         1085 a          997 c          765 g         1287 t
ORIGIN      
        1 ttcaacatca gcatcttcaa gtacgatgaa aggatcactt ccaccaagtt cgagaacgaa
       61 ctttttcatg ttctgtccgc aaatttctgc caccttttct cctgcagaaa cgctccctgt
      121 caatgatact gcatctatct cctttctctc gataagcgac gatgctgttg gcccatcaat
      181 aagcagcgtc tggaaaaccc cttcgggaaa tccagcatct gaaaatactt tttcaatttc
      241 aagtgcacat aatgggacgt agcttgaatg tttaagaaga acaacatttc ccccggaaag
      301 agcaggagca gcaaaacgtt gcaaaatact caagagccca ggcacatttt tcaatctctg
      361 atattgattc ttttatgggt ttacccattt ccattgtgat gattttgcca agttcctgct
      421 ttcttcttcg taatactttt gcggcgttaa tgagatgttt tcctctctta gatatgtcaa
      481 gccttttgca gtccgaaaat gcatcttttg atctcttaat cgattcattt atcctttctt
      541 gcgaataaag ctcaaattcc ctattcacat ccccggtagc aggattaata gatacaatct
      601 ttttcataga tttcatcttc ttttgttatc ttgccaatca aatgatcaat ttttcctttt
      661 caacttcccc cctaaaaatt gctttttcgg tgagaatatt caccgcccta tctctcaagc
      721 agatttgatg agtgggaacc cgaactatcc tgcttacttt tttaatatgt ttctcaggaa
      781 cgtatgcaga attccgccat ttctataaaa ttccacttct atgggggcat ccaaccttga
      841 tatgaccttg aatttcgttt cttttccatc agctttcgca accacatgca gttcttctcc
      901 cgggaaaata ttactgatgc caagaatgtc aaatacctca tcacctttta gtcccagagt
      961 atctgcattc tctcctttct caaactgcaa cgggagaaca cccattccca gaagattgct
     1021 cctgtggata cgctcaaaag attcagctat cactgctatg accccgagaa gataagttcc
     1081 tttggcagcc caatcgcggg aacttcctgt gccatattct tttcccgcaa tgatgataag
     1141 tggtgtctgt ttctgtttgt aaagcatcgc cgcatcatag atggtcattt cctcaccttt
     1201 tgggaaatag cgcgtccagc ccccttcctt ctctggaata agcatgttct tgagattaat
     1261 atttgcaaat gttccccgca tcatgacttc atgatttccc cttcttgatc catacgaatt
     1321 gaaattttcc ggtgacaatc cctttgagat caaatactgc cctgcaaggc tatcttccga
     1381 aaattcacca gctggagaaa tgtgatctgt tgtgatacta tttcccaggt atgcaagtac
     1441 cctggcacct gatatgtctc ctttttcagg aggtacaacc ggaaaatcca tgaaaaaagg
     1501 gggttcctgg atatatgttg atgtcggctc ccattcatag agcttacctg ttggtacgat
     1561 aagttcctcc cagagtgttg aaccatgcga tatttgcgaa tactgttttt catagagcat
     1621 cgggtcaata gtattctcta ttatgtgtct gatatcttcg ctatccggcc agatatcctt
     1681 taaatatact ggattattat tgggatcgaa tcctataggt tctgttttga gatttatatc
     1741 tattgtacct gatatggcat aagcgaccac gagaggaggc gagaccaggt aatttgcttt
     1801 aacaagcggg tttatcctcc cttcaaaatt acgatttcca cttaaaaccg ccgcaactat
     1861 caggtttttc tccttgactt cttttgctac catatcctgt atggggccac tgtttcctat
     1921 gcaagtcgtg cacccgtaac ccacaagatg gaatcccaga gcctcaagat atggcatgag
     1981 ccctgacttt ttcaggtact ctgtgacaac aagtgaacca ggagcaagac tcgttttcac
     2041 gtatggtttg accttgagcc cgcgctccac agctttcttg gctaaaagac ccgcctctat
     2101 catcactgtg gggttcgaag tgttggtgca tgaggttatt gccgcaatta cgatcgagcc
     2161 gctttttatt gggactttca tttcatactt ttcttcaact tgcataagac caatgcgttt
     2221 atgctcatca ctgaaacttc ctccctcccc aagccagcga tgatattcat gatctgcttc
     2281 cacatctttt ttctttctcc tgaaagtctc ttccatggcc ttatggaaac tttcttccaa
     2341 tttgtcaaga ggaatacgat cctggggtcg ttttggcccc gcgaggcttg gttcaaccgt
     2401 actcatgtca agttccaatg tctcgctgaa caggggttcg ggactttcat cggtatggaa
     2461 aagaccctgt tcttgagtgt actttttaat taggtctaca tcttcttttt tcctcccagt
     2521 cccaaggaga tactttattg tttctgcacc aacgggaaag aatcctgcgt ttgcaccata
     2581 ttcaggagcc atatttgcaa tggtggccct gtcgggtagg ctcaatttgc tcaatcccgg
     2641 cccgtaaaat tcaacgaatt ttcccaccac attatgttcc cttaatatct gggtgatcgt
     2701 gagaacaagg tcagtagatg ttactttctc cttaatctca cctgatagtt taaaacccac
     2761 cacatccggt acaggcatat ggtatggctg accgagcata accgcttctg cttctatccc
     2821 tccaacgccc caccccagaa ctcccatgcc attgatcatc gtggtacagc cggtattact
     2881 ggatttatgc atgcagggtc acctcctaac ctcaaaactt ccgacctcat tgcggccaga
     2941 tccgcaacca gaggaacacc ggtgaagtcc tgaaggagaa ctctggccgg tataaagggg
     3001 atctcgtgtt catttctctt tttcttgtag tattgggcac atgatttaac gtcctcttca
     3061 gtaataagtt tcccatccat ttgccgcagg agtgattcaa gcaatatctt tatggaatag
     3121 ggaagacggg agacgttttc aaaacccatc tcttcaagcc tgttgagtcg atatatgaca
     3181 acaggaccct ttgacgtatc aataacatct ttgatcttag aaaaatctat actcataaca
     3241 ttttattcac ttattcaatt tttcaagcct tctggcagct tcctgtaatg tctcatcttt
     3301 cttagagaac gtaaagcgca gcttatgttt tcccggttcg ctgtgataga aactggttcc
     3361 agggacggct gcaaccccaa tatacgcaaa aaggattgac gatatatcta tgatttccat
     3421 attgaatcaa cccacttccc acctataaac attttgtatt tttttatcat agattcatct
     3481 caaatccaaa tggcagctcc atgatattcc gaataattct cacaagttta tctaaatgat
     3541 actatcttta gaggaacttt ttataagtct aagagaacat tccaacccta atattgctgc
     3601 cgcaaacagc acattgatct aatgaaaaga ttgtggttta tttgataaat agctttctga
     3661 ttatcacatc aacctttttg aaactctgct ttgagatgaa ttccagagct tgactcagtg
     3721 ccacagtcta tttcattagc ctgaacatga aatttgtcaa tagatcgaag ggtttcataa
     3781 ccaggaatga ataattccca aacctcttct tcatctcaaa ctgcgcctcc ccttttaaaa
     3841 tcgcgaactt catcttgttc tcagagaagt ctgcatcaaa gataaggaca tgatcagaga
     3901 taaggctagc tgtgacttcc cattcttttg agatccttcc attcaccctc tcataaaggg
     3961 cagtcccggg caaaggataa ggcatggtaa aggagaggta atcaagagga agagagacgg
     4021 caaaacgaag agtttcaagt acggtatcat cagtatctcc cggatagcag aggatgaaaa
     4081 acgctccggt cgatagaccg gcttcatggg ctgcataaac tgccttgcgc gctc
//
LOCUS       DATGBN010000213         7402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_133496, whole genome shotgun sequence.
ACCESSION   DATGBN010000213 DATGBN010000000
VERSION     DATGBN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133496"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VKL21_05100"
     CDS             <1..367
                     /locus_tag="VKL21_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMB45178.1"
                     /translation="KNDPGMAGVVNNSLKIVFSKTLRNVEEVPNWKNIRLFHEIKREE
                     IIKLKEQEGEDFTILGSGTIVQQFANLGLIDEYQFVIVPVILGAGKSIFKDVKKMNLK
                     LLEAKTFKNGIVLLKYRSA"
     gene            425..646
                     /locus_tag="VKL21_05105"
     CDS             425..646
                     /locus_tag="VKL21_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45179.1"
                     /translation="MSTKQMTIWFSTISIILVLWGIVFAFFGLDILPVKNRDILLPWE
                     SALYGAIMMGWGELFSLLAGLPLAEMTRN"
     gene            646..810
                     /locus_tag="VKL21_05110"
     CDS             646..810
                     /locus_tag="VKL21_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45180.1"
                     /translation="MKAMLYGIVLWLIVEGLFSAYLGVWFNVGVDIAVLVLFSFPIIK
                     ALRSPKEKNL"
     gene            827..1336
                     /locus_tag="VKL21_05115"
     CDS             827..1336
                     /locus_tag="VKL21_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMB45181.1"
                     /translation="MTKNNPIKKQDLVVTRIIDAPVELVWKAWTDPEYVMRWWGPKDY
                     TSPSCKIDFREGGKYLFCMRAPKDQGGQDMYTAGVYKKIVPMERLEFTQSMADKDGNR
                     IDPAELGMPPDFPKEMRMVIVFKSKKCDMTELSITHYDWTVSDGGQMYVYALAGWHQS
                     IDKLAESLK"
     gene            1791..2222
                     /locus_tag="VKL21_05120"
     CDS             1791..2222
                     /locus_tag="VKL21_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMB45182.1"
                     /translation="MDEFIEAAIQEAKKSSAQGGIPIGSVLVKDGKIIGRGHNQRVQK
                     DDPMAHAEIDCLRNAGRIGSYKDTVLYSTLMPCYLCGGAVVQFGIKKVIIGESRTFEG
                     SEEFMKQHGVEVINLDNNECITMMQDFIEKNHDLWFEDIGK"
     gene            complement(2176..2742)
                     /locus_tag="VKL21_05125"
     CDS             complement(2176..2742)
                     /locus_tag="VKL21_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMB45183.1"
                     /translation="MEFIEFQLHECYKFIRSNFKCERCGRCCVCINPIEITQIEAQEI
                     SKKEKKPVSEFVNGLSIKKEKYEPCPFLEGKDCSIYDLRPASCKTYPFLQITKRSKEY
                     SSKYHKVIIGGVPPKCPAILKVLSLFEKNFMAKIIFSIEEVQMKEILREYSGILVLVA
                     FEDIECTKRLENMVIYRYLQTTGHGSSQ"
     gene            complement(2871..4190)
                     /locus_tag="VKL21_05130"
     CDS             complement(2871..4190)
                     /locus_tag="VKL21_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HMB45184.1"
                     /translation="MLNLVKIIDSVNELTLNRFNISQKIAYGFIVVDILWLIVGYIGY
                     YDQNIVTFIDLDKTIFIFISFALFSSILMYIGLTRSVMKPLDELNIAANRISEGDLTL
                     DIEVSSGDELGTLAGYFKKMIYSLRSLTGKMKDTSFKVASKAHELSASSQCMRVSIDQ
                     ITNNTQGIAEGSSLQSVKMEKINQTVNEMSLIMQQVTVGSQRTAQATKEANTTAKELR
                     QKSHDLMDKITDIQDSVNKSALVIKNLDTNSEKIGEIVGVITNISDQTNLLALNAAIE
                     AARAGEHGRGFAVVANEVRKLADESRNSARKITELIKEIQQETKKAVGNMEQGTRTVM
                     DGSRMIEDTVLSIESIEQATENAANMFTEIIEIGKMQDDSMKNVKSSFDDISTVNRRS
                     AMANEEAVAGIQEQAASMNMLADISRELEELSVELQKDIAVFKQKEH"
     gene            complement(4345..5076)
                     /gene="hisA"
                     /locus_tag="VKL21_05135"
     CDS             complement(4345..5076)
                     /gene="hisA"
                     /locus_tag="VKL21_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HMB45185.1"
                     /translation="MFRVIPAIDIKNSKCVSLVQGIPGTERVSISNPLAVALRWVGGG
                     ARIIHLIDLDGAIEGHRSNSAIIESIIRNSRVETQVGGGIRSKKDAQDLLSLGADRII
                     LGTAAIKQPSLVKELAEEFGGNRIMVALDSKNGRVTTHGWAEKSAFTAVELGRMFEEL
                     GAGSILFTDINTEGLLEGVNPLPTQELVDALGIPVIASGGITTLSDIELIKETGAKGI
                     VIGAALYVGNFTLNEAMEFEGELPS"
     gene            5510..6142
                     /locus_tag="VKL21_05140"
     CDS             5510..6142
                     /locus_tag="VKL21_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMB45186.1"
                     /translation="MESAQLLDILGNENRRKILHLLASRPCYMSEIAERLDVGAKAIL
                     GHLELLVQAGMVEANVDEQRRKYFHITDNLRLEVFVSPYSFDVEITTLALSAPREHAP
                     AAQDVIKGLKSHYGQIRELLDKRHNVMQEYQHIQGNITEAMGHCMDAIEDVAQDNIEA
                     EILYALIKSPMNARVLSMQLGIPEYVIEGYIEEMEHRKILKRDENNYIIG"
     gene            6185..6679
                     /gene="hsp20"
                     /locus_tag="VKL21_05145"
     CDS             6185..6679
                     /gene="hsp20"
                     /locus_tag="VKL21_05145"
                     /inference="COORDINATES: protein motif:HMM:NF041800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HMB45187.1"
                     /translation="MERDWRKTEKGFFTEFENEIEQMNDLVNRMMQSMGKEPQVYGFS
                     IQVGPDGIPNVERFGNVIPVGKDRDVREPFTSSLVNEKKNELNITAEMPGINKEDIEL
                     NATEKEIIIKADSNARKYYKFIKTPDSVDPDSAAAKYNNGVLEITFKFKDMKKPEGKP
                     IKIE"
     gene            6718..>7402
                     /locus_tag="VKL21_05150"
     CDS             6718..>7402
                     /locus_tag="VKL21_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HMB45188.1"
                     /translation="MDKKDKALTLKVIEAKSYDAGRGIARIDPEVAYDLGLQTGDVIG
                     IEGTKRTAALVWPGYPEDSKSGLIRIDGTVRRNAGVSIDDKVQIRKIHTAPAEKILFS
                     PTQPLKIQGGEAYLAHNLEGRVITRGDVVELNIMGRRVDLVVVSIKPVADSVIIGGRT
                     VIEISDKPAKEMPSIPRVSYEDIGGLGDEVRKVREMIELPLRHPELFERLGVEAPKGV
                     LLHGPPGTGK"
BASE COUNT         2274 a         1467 c         1554 g         2107 t
ORIGIN      
        1 caagaatgac ccgggtatgg ctggggttgt gaacaatagc ctgaagatag ttttttcaaa
       61 aacgctgcgg aatgtggaag aagtacccaa ttggaaaaat atcaggctct ttcatgaaat
      121 aaagcgggaa gaaattatca agctgaaaga gcaggagggc gaagatttca caatattggg
      181 tagcggtacc atcgtgcaac agtttgcaaa cctcggtctc atcgatgaat accaatttgt
      241 gatcgtcccc gttattttag gcgctggcaa atccatattc aaagatgtta aaaagatgaa
      301 cctgaagctt ctcgaagcaa aaacatttaa aaatggaatc gtcttgctta aatacagatc
      361 cgcctgaaag cgtggagggt ttagcatgga ttgagaattt gccacatttg ccaggtaaca
      421 aaatatgagc acaaaacaaa tgacaatttg gttcagtacc atatccatta ttcttgtact
      481 ctgggggata gtgtttgctt tctttggtct tgatattctt ccagtaaaga acagggatat
      541 tttgttacca tgggaaagcg cactttacgg cgcaattatg atgggctggg gagaactctt
      601 ttcattgttg gcaggattgc ctttagcaga aatgacacgg aactgatgaa agccatgctg
      661 tatgggatag ttctgtggct catcgttgaa ggattgtttt cggcttatct tggcgtctgg
      721 ttcaatgtgg gagtagatat tgctgtatta gtattgttca gcttccctat catcaaagcg
      781 cttcgctctc ctaaagaaaa aaacttatga aaagaaggtg gaacctatga caaaaaataa
      841 tccgataaaa aagcaagacc tcgtcgtgac ccgcattatc gacgcaccag tcgaactcgt
      901 atggaaagcc tggacggatc ctgagtacgt gatgcgctgg tggggaccga aagactacac
      961 ctccccctca tgcaaaatag attttcgtga gggcggcaaa tatctatttt gtatgcgcgc
     1021 gccgaaagat caaggtggtc aagatatgta cacagctggt gtctacaaga agatcgtacc
     1081 aatggaacgc cttgaattta cccagagcat ggctgacaaa gacggcaaca gaattgatcc
     1141 cgctgagttg ggcatgcctc ccgacttccc aaaagagatg cgcatggtaa tcgtctttaa
     1201 atccaaaaaa tgcgatatga ctgaactttc catcacgcat tatgattgga cggtgagcga
     1261 tggtggtcaa atgtacgtct atgccctggc aggttggcac cagtcaatag acaagctcgc
     1321 tgaaagccta aaataattag aacgtatgaa taagaacact ttgaaaatca taatgctggt
     1381 gtaatatcgt ttgataatcc tgaccagacc gctttaagac attcaatagt tttaggtgat
     1441 gtaagtttcc acaatgtctc cgatgaaata gaaaacgaac ccgaaaatga atagatacca
     1501 tcttgatgca ggtgaaaaag gcaacatcat ggtgaccgtt acaattagtg ttggtgaata
     1561 aaagcgaact tgactggtgg tgtacaacaa atcgtgacct ttgacaggat ttacaggatc
     1621 gacaggatac ggttagtgtg ggaaatcctg taaatcctgt cgatcctgtc aaaaaaaata
     1681 atgatgcaaa aaattatacc aacatgtaat gtaacggtca cacatgatgc aacatgatat
     1741 atgcatatat aataaaatta tggataattt ccctatttgg acggtaatat atggacgaat
     1801 ttatagaggc agcaatccaa gaagctaaaa aaagttccgc tcagggagga ataccgattg
     1861 gttcagttct tgtgaaagac ggtaaaataa tagggcgagg tcacaaccag agagtacaga
     1921 aggacgaccc catggctcat gcagagattg attgtttgag aaatgcaggc aggattggga
     1981 gttataagga tacagttctg tattccacgt taatgccatg ttacctttgc ggtggtgctg
     2041 ttgtccaatt cgggattaag aaagtgatta tcggtgaatc cagaacattt gaaggctctg
     2101 aagaatttat gaaacagcac ggggttgaag taataaacct tgacaataac gaatgcatca
     2161 ccatgatgca ggactttatt gagaagaacc atgacctgtg gtttgaagat atcggtaaat
     2221 aaccatgttt tctaatcgtt tagtacattc aatatcttcg aatgcaacta agactaaaat
     2281 gcctgaatat tctcttaata tctctttcat ttgcacctct tcaattgaaa atataatttt
     2341 tgccataaaa ttcttttcaa acagactgag aactttcaat atagcaggac attttggtgg
     2401 aaccccacca ataatcacct tatgatattt cgatgaatat tccttacttc gctttgttat
     2461 ttgtaaaaag ggatatgttt tacaggatgc gggtcttaaa tcatagatgg aacaatcctt
     2521 accttccaaa aatgggcacg gttcatattt ctcctttttt atagatagtc catttacgaa
     2581 ttcagaaact ggttttttct ctttcttaga aatctcttga gcttctattt gtgttatttc
     2641 tatcggattt atgcaaacgc aacaccgtcc acatctttca catttaaaat tgcttcgtat
     2701 aaatttataa cactcatgta actgaaattc tataaattcc ataatttatt tgattcattg
     2761 attataagcc tatgcatact gaatcttgaa tgatgggtct gagttgcgcg aagttcgcac
     2821 atcatgtcga ggactttcaa taaaaaattt atactcccgt tgtgattctt ttagtgttct
     2881 ttttgcttga ataccgcgat atctttctgt aattctaccg acaattcctc taattcccgt
     2941 gagatatccg caagcatatt catagatgct gcctgttcct ggattcctgc tactgcttcc
     3001 tcatttgcca tggcagaccg tctgtttacg gtagaaatat catcaaacga ggattttaca
     3061 tttttcatag aatcatcctg catttttcct atttcaatta tttcagtgaa catatttgct
     3121 gcattttcgg ttgcctgctc tatactctcg attgataata ctgtatcctc tatcatcctg
     3181 gaaccatcca ttaccgtcct tgtaccctgc tccatgttcc caaccgcttt tttggtttcc
     3241 tgttgtatct cttttataag ttccgtgatc tttcttgccg aattccttga ttcatctgca
     3301 agttttctga cttcatttgc caccactgca aatcctcttc catgctctcc ggcccgggct
     3361 gcttcaattg cagcgttaag ggcaagaagg tttgtctggt ctgagatatt cgttattaca
     3421 cctactattt cacctatctt ttcagaatta gtatcaagat tctttataac aagagcagac
     3481 ttatttactg agtcctgaat atccgttatc ttatccatca ggtcatgtga tttctggcgc
     3541 aattcttttg ctgtagtatt agcttctttg gtagcctgtg ccgtcctttg cgatccaact
     3601 gtaacctgct gcattatgag tgacatctca ttaacagtct gatttatttt ttccatttta
     3661 acagactgca ggcttgatcc ttctgctatc ccctgtgtat tatttgttat ctggtcaata
     3721 gaaactctca tacactggct tgatgcagaa agctcatgtg ctttagatgc tactttaaat
     3781 gaagtatcct tcatttttcc agttaagctt ctgagtgaat aaatcatttt cttgaaatat
     3841 cctgccagag ttccgagttc atccccggat gaaacctcta tatctaatgt aagatctcct
     3901 tcagaaattc tatttgcagc gatatttaat tcatctaatg gtttcatgac agagcgtgtc
     3961 aatcctatat acatgagtat ggatgagaaa agagcaaagg aaatgaaaat gaagatggtc
     4021 ttgtcaaggt cgataaatgt gacgatattc tgatcataat acccaatata tcccacgatc
     4081 aaccataata tatcaactac tatgaaacca taagcaattt tctggctaat attgaacctg
     4141 ttaagggtca gttcatttac gctatctatt atcttcacta gattcaacat aaatcactca
     4201 caaataagtt ataatattat tttaaataat aaagaatcga ttctctcctt aatatatgaa
     4261 tttgtgataa aagtgtgaaa tatgcatttt gctttccatg gtttggattt aaatttgttt
     4321 tgatattcag ttccacaaat acgcctaaga tggtagttca ccttcgaatt ccatagcttc
     4381 attcaaagta aaattaccta cataaagggc agccccaata acaatgcctt ttgctcctgt
     4441 ttctttgatt aactcgatat ctgataacgt ggtaattcca cccgaggcta taacagggat
     4501 tcccaatgca tccacaagtt cctgcgttgg caacggattt actccttcca gcagtccttc
     4561 agtattaata tctgtaaaga gtatggatcc agctccgagt tcctcgaaca tcctgcccag
     4621 ttcaacagct gtaaaagcgg atttttcagc ccagccatgt gttgtgaccc tgccattttt
     4681 tgaatcgagg gcaaccatga tcctattgcc tccgaattct tctgcaagtt ctttaaccag
     4741 agaaggttgc tttattgcag cagtacccag aattatcctg tcagcgccta atgataggag
     4801 atcctgtgca tcctttttag accttattcc tccgcctacc tgcgtttcca ctctggaatt
     4861 cctgataatt gattcaatta tcgcactatt ggaacggtgt ccttcaatgg cgccgtcaag
     4921 gtcgatgaga tgaattattc tagcgccacc ccccacccag cgcaaagcaa cagctaaagg
     4981 gttggaaatg gatacccttt cagtacctgg aataccctgg acaagagaaa cgcatttact
     5041 attcttaata tcgatcgcgg ggattactct gaacatatta ccacaatttt atacgaccat
     5101 agggcgcatt gtcagcaacc tcacgctcaa agctgtgagg cttgtcattc cagttttcgt
     5161 taaggggttt gtaccatatg acgcttgcaa aattgcatag aactctactg aatcaaagaa
     5221 tgtactggag tatattaaca tgaatgaata taaagataac gaatcaaata agaagcgggg
     5281 tacgcttcct cccgtcgcta aagctcaggg cttccgctac ccctgcaccc caagggattg
     5341 tcaaagctct gtgctctttc gaactctgtg gttgattttc cttgcgacga aacattcttc
     5401 cggaagaaat taaaaaatct ccggtaaaca cgagggtaaa gtatatatac catacacact
     5461 aacttaaggt tagtaacctc aagtcactaa ctcggggtga agcacaaaaa tggaatcagc
     5521 acaactgtta gatatacttg gaaatgagaa ccgaagaaag atcctgcacc ttctagcaag
     5581 ccgcccatgc tatatgagcg agatagcaga aaggcttgat gttggcgcaa aggcaatact
     5641 cgggcatctt gagcttcttg tgcaggcggg tatggtcgaa gctaacgttg atgagcagcg
     5701 gcgcaaatac ttccatataa cagataacct ccgtcttgag gtatttgtgt cgccatattc
     5761 atttgatgta gagataacca ctcttgccct atccgcacca agagagcatg ccccggcagc
     5821 ccaggatgta atcaagggct taaaatccca ttacgggcag atacgggagc ttctggataa
     5881 gaggcacaat gtaatgcagg agtaccagca catccaggga aacataaccg aagcgatggg
     5941 gcattgtatg gatgccatag aggatgttgc gcaggataat attgaagcgg aaatactata
     6001 tgccctcata aaaagcccga tgaatgcgcg tgtgttgtcc atgcagcttg gaattccgga
     6061 atatgtgatc gagggatata ttgaagaaat ggaacataga aagattctaa aacgagacga
     6121 aaataattat ataataggct aaagaatata aaatatacaa aaaattagaa aaagaggaaa
     6181 aaatatggag agagactgga gaaagactga aaagggattt ttcactgaat ttgaaaacga
     6241 gatagaacag atgaacgacc ttgttaaccg catgatgcaa tccatgggaa aagagccaca
     6301 ggtttatggt tttagcattc aggtgggacc tgacggcata cccaatgtgg aacgtttcgg
     6361 gaatgtcata cctgtcggta aagaccggga tgtcagagaa ccttttacaa gctcattagt
     6421 taatgaaaaa aagaatgaat tgaatattac agcggaaatg ccgggaataa acaaagaaga
     6481 tatcgagctc aatgcaaccg agaaagaaat tatcatcaaa gcagatagca atgcaaggaa
     6541 atattacaag ttcataaaaa caccagactc ggttgaccct gacagcgcgg cagcaaagta
     6601 caacaatggg gtgcttgaaa taactttcaa attcaaagat atgaaaaaac cggaaggaaa
     6661 gccaataaag atagagtaaa tcaaagatag aacttagata tttatataaa gggaaatatg
     6721 gataaaaaag ataaagcttt gactctaaaa gtcattgaag caaagtccta cgacgctgga
     6781 cgcggtattg ccaggattga tcctgaagtc gcgtatgacc tggggttgca aacaggagat
     6841 gtcataggca tagagggcac caagaggact gctgcccttg tatggcccgg ttatcctgaa
     6901 gacagcaagt cgggattgat acggattgac ggtacggtaa gaagaaatgc aggagtgagt
     6961 atcgatgata aggtacagat ccgaaaaatt catactgcgc ctgctgaaaa aatattgttt
     7021 tcaccaacgc agcccctgaa gatccagggc ggagaagcat atcttgctca taaccttgaa
     7081 ggacgcgtaa ttacccgcgg ggatgtagtg gaattaaata taatgggccg ccgcgttgac
     7141 cttgtggtag tatcaataaa gcctgttgcc gattcggtga ttatcggagg acgaaccgta
     7201 attgagatca gtgataaacc tgcaaaagag atgccttcta tccccagggt atcatatgaa
     7261 gatataggcg gactcgggga tgaagtaagg aaggtaaggg aaatgataga gcttcctttg
     7321 aggcatcctg agcttttcga acgtctcggg gtagaggcgc caaaaggggt tctccttcac
     7381 gggcctcctg gcacagggaa aa
//
LOCUS       DATGBN010000214         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_560039, whole genome shotgun sequence.
ACCESSION   DATGBN010000214 DATGBN010000000
VERSION     DATGBN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560039"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VKL21_05155"
     CDS             complement(<1..452)
                     /locus_tag="VKL21_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HMB45189.1"
                     /translation="MDLIAKRVKTITWIIKAFAILGLFLIYPVLAAYPDMNLVANPGF
                     ENGTTTLLNWTFVSTNENTPVWSNISHSGSKSIEINIPGTTDLQSGYPESDRIIVQPF
                     TTYTFSAWGKTQGADGANLPMITLGELDADKNLLNLTNLPLFSRGTND"
     gene            770..2056
                     /locus_tag="VKL21_05160"
     CDS             770..2056
                     /locus_tag="VKL21_05160"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMB45190.1"
                     /translation="MVLEISKDTEFADAFTWISKGNTHVESGMYTEAIQCYDRALEIN
                     PGMADVWNNKGLAMARISKYEDAVSCYDKAIEFKPDDEEVIYNKGIALAKLGRTTEAI
                     ACYDKILEKDPMDAGAWCIKGDILFESGNFEVALRAYDRSIEIDPKDETVWNNRGLTL
                     AKLDRLSEAIESYDKALEINPKVEKIWSNKGLAIAKMGQAGEKIDLQKIASAMPEEEK
                     ALEELPACIQPEQIVSQEVTGAAKSLSEPLIIKPLETVIEIPTKEPENVVVIKVENQE
                     NLISEIKVNKKEIARHEINEESKDYLIKGNSFFSKRKYDQATDYFDISLRVDAQNSIA
                     WNNKGLALARMGKIDESLICYEKALEITPEDHVFLINMGNILYKKGNNREAFKAFESA
                     FELAPESSNAIKGMELCLKSLKKSLKKKKLYNGRNQ"
     gene            2040..2903
                     /locus_tag="VKL21_05165"
     CDS             2040..2903
                     /locus_tag="VKL21_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMB45191.1"
                     /translation="MEEINKNLKIAIILTSLIFFLEVAGGIISNSLALLSDAAHVFMD
                     VVALLLTFGAIRISGRPSNGSVTFGYHRFEIFAALINGLTIIGIAVIICYEAYERILD
                     PPIVKGFEVLLIATTGLIANAWVAIKLHGHHDLNIKGAYLHVMGDALASIAVIAGAVV
                     IIFTGNNIIDPILSIVIGLMLLYGAFKLVFGSIRILLEFAPKHVDADTLTKVMMEIEG
                     IKGVHDIHIWSICSNIHALSAHVLVDRIHVEQTEVLIIQINRTVRERFRIIHTTLQFE
                     CAECVPVELWH"
     gene            complement(2900..3388)
                     /locus_tag="VKL21_05170"
     CDS             complement(2900..3388)
                     /locus_tag="VKL21_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HMB45192.1"
                     /translation="MDPNPEKIIIGIAELVVVHNPAILITIGLGSCVAISIRDPVARF
                     GGLSHILLPSISESNNKSNRMKFADSAIEMLVDMLLQKGCLKSRLEAKIAGGASMFNI
                     SGNTINMGERNTEAVKKKLKELKIPLLASDTGANYGRTIEFNISSGIMYVKSAFHGIK
                     EI"
     gene            3695..>4250
                     /locus_tag="VKL21_05175"
     CDS             3695..>4250
                     /locus_tag="VKL21_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HMB45193.1"
                     /translation="MVNTTEPKKTIKIENVVASTAIGAKLDLIQVISVFEGAEYNKER
                     FPGVVYRTKSPKTAALIFSSGKIVCTGAKSIADVGIGLSNVFDKLREMGVKILDKPEI
                     KIQNIVASADLGRVLNLNAVAIGLGLENIEYEPEQFPGLVYRMSSPKVVMLLFGSGKT
                     VITGGKKPEDAEVAVEKIVIELDGL"
BASE COUNT         1370 a          770 c          854 g         1256 t
ORIGIN      
        1 tagtcatttg tacctctgct aaataatgga aggttagtca gattcaacaa attcttatcc
       61 gcgtccagct ctcctaatgt gatcattggt aaattcgcgc catctgcccc ctgggttttc
      121 ccccatgcag agaatgtata agttgtaaat ggttgcacaa ttattctgtc cgattccgga
      181 tatccggatt gaagatcggt tgttccaggg atgtttattt ctatagattt tgaaccgctg
      241 tgggagatat tggaccatac cggtgtattt tcatttgtgg acacaaatgt ccagttcagg
      301 agagttgttg ttccgttttc aaagcccgga tttgcaacaa gattcatatc cggatatgct
      361 gctaaaactg ggtaaattag aaagagacct aaaattgcaa atgcttttat gatccaggta
      421 atagttttaa ctcttttcgc tattaaatcc atgtattatc tccgtttttt acagaaattt
      481 tctaatatcc tatccaatta tccaaattat tattaatcgt atatacacga acaagatatt
      541 tatagtttat gattggcgta aacccaatat cataattaat tatacaatct cttaactatt
      601 caaataacat tcaatgatta aaatgtatcg atatttttca aggttctttc agtttttgtg
      661 ctcacaaatt tttcttggat ttatataata tttgaatcca acggagttaa ataggataag
      721 caatattaat taattcatta tatatataag actgatatat ggagtttcaa tggtactgga
      781 gatatccaaa gatacagaat tcgcagatgc ttttacatgg atcagcaaag gcaatactca
      841 tgtggaaagc ggtatgtata ctgaagccat ccaatgttat gatagagctc ttgaaataaa
      901 tcccggaatg gctgatgtat ggaataataa aggccttgcc atggcaagaa tttcaaaata
      961 tgaagacgct gtatcatgtt atgataaagc aatagaattt aaaccggatg atgaagaagt
     1021 tatttacaat aaaggcatcg ctctggctaa gcttggaaga acaacagaag ctatcgcctg
     1081 ttatgataaa atacttgaaa aagatccaat ggatgctggc gcatggtgta taaagggaga
     1141 tatattattt gaaagcggga actttgaagt ggctcttcgc gcttatgacc ggtccataga
     1201 aatagatcca aaagatgaaa ctgtctggaa taacagggga ttgactcttg caaaactcga
     1261 tcgcttatca gaagcaatag aatcttatga taaagctctt gaaatcaacc ctaaggttga
     1321 gaaaatatgg agtaataaag ggcttgcgat cgcaaaaatg ggacaggctg gtgaaaagat
     1381 cgatctgcaa aagatagctt ctgcaatgcc agaagaagag aaagcgctag aagaactacc
     1441 cgcttgcatc caaccggaac aaattgtgtc gcaagaggtt actggggccg ctaaatccct
     1501 atccgaacct ctaatcataa aaccacttga aactgtaatt gaaatcccca caaaggaacc
     1561 agaaaatgtg gttgtaataa aggtagaaaa ccaggaaaac ctcatatctg aaattaaagt
     1621 caataaaaag gagatcgcaa ggcatgaaat caatgaagaa tcaaaggatt acctgattaa
     1681 aggaaattca ttcttctcaa agcgaaaata cgaccaggcc acagattatt ttgacatatc
     1741 gctcagggta gacgcgcaaa atagcatcgc atggaataac aaaggacttg cgcttgcaag
     1801 gatgggaaaa attgatgaat cacttatctg ctatgagaaa gcgcttgaaa taacccccga
     1861 agaccatgtt ttcttaataa atatgggtaa tatcctttat aaaaaaggga ataacagaga
     1921 agcatttaaa gcttttgaat cagcttttga actggctccg gaaagcagta atgcaataaa
     1981 agggatggaa ctttgtctta aatcattaaa aaaatcctta aagaaaaaga aattgtataa
     2041 tggaagaaat caataaaaat ttgaaaattg caattatcct gacttcactc attttttttc
     2101 ttgaagttgc aggcgggata atttccaata gtcttgctct cctctcagat gcagcacatg
     2161 ttttcatgga tgttgtagcc ttattgctca cctttggggc gatcaggata tcagggcgcc
     2221 catccaacgg aagtgttacc ttcggttatc acagatttga gattttcgcc gcccttatca
     2281 acggattgac aattatcggg atcgcagtta tcatctgtta tgaggcatat gaaaggattt
     2341 tagatccccc tattgtaaag ggctttgaag tgctattgat cgcgactaca ggtcttattg
     2401 caaatgcatg ggttgctata aaattgcacg gacatcatga cctcaatatt aaaggagcat
     2461 acctgcatgt tatgggtgat gcccttgcat caatagctgt gatagctggc gcagtggtca
     2521 tcattttcac cgggaataat ataatagacc ccattcttag cattgtaata ggtttgatgc
     2581 tcctttatgg ggcattcaaa ctcgtttttg gttcgatccg gatacttctt gaatttgctc
     2641 cgaaacatgt ggatgctgat acccttacaa aagtgatgat ggaaattgaa ggaataaaag
     2701 gagtacatga tatccatatt tggagtatat gttcaaatat tcatgccctg agcgcgcatg
     2761 tgcttgttga caggatccat gtagagcaga cagaggtctt aataatacaa atcaaccgga
     2821 ctgtcaggga aagattccgt attattcata caacattaca attcgaatgt gccgaatgtg
     2881 ttccggtgga attatggcac taaatttcct taattccatg aaaagcgctc tttacataca
     2941 tgatccctga agaaatgttg aattcgatcg tccttccgta attcgcaccc gtgtcactgg
     3001 caagaagcgg gattttcaat tctttgagtt tcttcttaac cgcttcagta tttctttctc
     3061 ccatattgat agtattgccg ctgatgttga acatgctcgc tccccctgca atttttgctt
     3121 ccagcctgct tttcaggcat cctttttgca gcagcatatc tactaacatt tcaatggctg
     3181 aatcggcaaa tttcatgcga ttacttttat tatttgattc cgatatactt gggagaagta
     3241 tatgggaaag tccgccaaac cttgcaaccg gatcacgaat tgagatcgcc acgcatgacc
     3301 caagaccaat agtaataagt atggcagggt tatggacaac cacaagttca gcaattccaa
     3361 taattatctt ctcagggttt ggatccataa cattagctat tcttttatct tgagatataa
     3421 ccaaacccta taataatgct catcattatc attgttttcc tgcttgattt atcatcaaat
     3481 tggcaaggat ttgggtataa tcatggaaaa ttgatttaaa tgcaggatag atagtaagta
     3541 gtagagggac cttctatcct atccataaac tatttttcac ggttcctcac ctatccataa
     3601 actattttga tgggcatata tccttaacaa gcaagaatcc ggaaggttca tcccaaccta
     3661 taaagcttta aataccctat ataaatatga aactatggta aatactacgg aaccaaagaa
     3721 gactataaaa atagaaaatg ttgttgcatc tacggctatt ggagcaaaac ttgacctgat
     3781 tcaagtcatc agtgtttttg agggagcaga gtataacaaa gaaagattcc ccggtgttgt
     3841 ttacaggaca aaatcaccga agaccgcagc gcttattttc agtagtggca agatcgtttg
     3901 tacaggagca aaaagtattg ccgatgtagg cataggtctt tcaaatgtgt ttgataaatt
     3961 aagggaaatg ggtgtcaaga tcctggataa gcctgaaatt aagatacaga atatcgttgc
     4021 atcagcagat cttggaagag tgcttaacct caatgcagtt gctatcggtc ttggtcttga
     4081 gaatatagaa tatgaacctg agcaattccc cggtcttgtt tacaggatgt ccagccccaa
     4141 agtggtcatg ctattgttcg gctctggcaa aactgtgata acaggcggca agaaaccaga
     4201 ggatgcagaa gtagcagtcg agaagatagt tatagaactt gatggattag
//
LOCUS       DATGBN010000215         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_366420, whole genome shotgun sequence.
ACCESSION   DATGBN010000215 DATGBN010000000
VERSION     DATGBN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366420"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1606
                     /locus_tag="VKL21_05180"
     CDS             284..1606
                     /locus_tag="VKL21_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMB45194.1"
                     /translation="MELIKIMDIVNEQSLNRFRIGMKIAYGFIVVDMLMLIVGYMGLY
                     SESIRAYIDPGKAIIVCILFAILSSILMCIGLTRSIMKPLKEFMNAADRIADGDLTTE
                     MYISSNDELGQLAEYFRKMTSNLRHLTGKVHNVSLKVLNTSHGLSASSEEMKTSSDQI
                     SSTTHDIAEGAGQQASKMAEVNRAMKEMSESVQQVALNSQKASEGASSASTTAQNAGK
                     MSVEVAQKMTDIRSTVDNSATVIKQLDSKSQQIGEIIGVITNIADQTNLLALNAAIEA
                     ARAGEHGRGFAVVADEVRKLAEESRGAANQITGLIKEIQQGTKQAVAAMEQGTKTVVD
                     GAKTMEDTVLAINKIVKAAADVAAMIHEIAAAAEEQSASVEEVTATVEDVSAICEQSA
                     AGTQETSAAAQEQAASMDQLFNAAQELARLSNELQTEISKFNIGESVR"
     gene            1603..2397
                     /locus_tag="VKL21_05185"
     CDS             1603..2397
                     /locus_tag="VKL21_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMB45195.1"
                     /translation="MKTKLLDILTFSEKRENLLILLREGPKSLEEIRSSLNVTSSGMI
                     PQIRKLEERNLVHRIGKNYELTDIGMIVTNAFFPLIKTMEFIEKHENFLKEHDIKAIP
                     VHLLTRIFEFENSSLTESKISELYEPHKEFMKYLRDSRKFLGIALVFHHSYPPLFLKL
                     AESGRQVSIILTKEVFEKVKKEYEVELSKFIGLENGQLFISNEIIRLASAVTDNFMSL
                     SLFFKSGGYDSQRELVSSDKTAIKWGEELFYHFIAHSKKVKNLKAL"
     gene            2412..2552
                     /locus_tag="VKL21_05190"
     CDS             2412..2552
                     /locus_tag="VKL21_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45196.1"
                     /translation="MNSPLTRVLRSDFLIQSPFSQFGLVQQMVLIIPANEDISSIAIS
                     GM"
     gene            2672..>3253
                     /locus_tag="VKL21_05195"
     CDS             2672..>3253
                     /locus_tag="VKL21_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMB45197.1"
                     /translation="MTSKENEFRDKVHQIEPLTTGLLENAQTDKAAMIAQSDSNNSNA
                     RNSVVILSIIAVLTCITIGIIISRFLRKFAGKVQDAVLKVGATAEELSASSQEMKAFS
                     EQISVTTQDIATGAGQQSMKMADVSRTMREMSMSVQQVAGNATKAADSADKAHITAKE
                     IGQKSQEVVKQMTEIRSAVDNSSIVIKQLDGKSQ"
BASE COUNT         1096 a          601 c          745 g          811 t
ORIGIN      
        1 acatcagcgg ccgcagaaga gcagtctgct tcgatggatc agcttgtgaa tgcagcccag
       61 gaaatggcaa aattgtccga agaattgcag gctgaagtga ataagttcaa tatcggtgag
      121 tcctgaaaaa atcagatgac atattagcaa aaggccgaat aaggccatct gcttttttta
      181 tgcttcacac gcttttcaca aaatctttta tatgtacgaa aatgcaattt tcatttaaat
      241 ataattcctg atcctgcagg aaaacaattc ttagaggtct aatatggaat taattaaaat
      301 aatggatatt gtcaatgaac agagtctaaa taggtttaga ataggtatga aaatcgccta
      361 tggcttcatc gtggttgata tgttgatgtt gatagtggga tatatgggtt tatatagtga
      421 aagcatcaga gcttatattg atccagggaa ggcaattatc gtttgtattc tattcgctat
      481 tctttcatcc atactaatgt gtataggtct tacccgttca atcatgaagc ctttgaaaga
      541 atttatgaac gccgcagaca ggattgcaga tggggattta acaacggaaa tgtatatttc
      601 ttcaaatgac gagctgggac agcttgcaga atatttcagg aaaatgactt caaacctccg
      661 tcatctgact ggaaaagtgc ataatgtctc attgaaagtt ttaaatacat cgcatggact
      721 ttcggcatca agtgaggaaa tgaaaacttc ctcagaccag atttcaagta ccacccatga
      781 tattgccgaa ggagcaggcc agcaggcatc gaagatggct gaggtcaatc gggccatgaa
      841 ggagatgtca gaaagcgttc agcaggtcgc ccttaactcc cagaaagctt cagaaggtgc
      901 aagttcagcc agcacaactg cccagaatgc aggcaagatg tcagttgaag tagcgcaaaa
      961 gatgacggat atccggtcaa ctgtggataa ttcagcaaca gtaataaaac agcttgacag
     1021 taaatcccag cagatcggcg aaataatagg cgtgataacg aatattgcag accagacaaa
     1081 cctccttgcc ctgaacgcgg ccatagaagc ggcgcgtgca ggagagcatg gaagaggctt
     1141 tgcggtggtt gctgatgagg tcaggaaact tgcagaggaa tcccgtggcg ctgcaaatca
     1201 gataacagga ttaataaaag agatacagca gggtacaaag caggctgttg cagccatgga
     1261 acagggcaca aagaccgttg ttgatggtgc aaagaccatg gaagatactg tcctggcgat
     1321 caacaagata gtcaaagcgg cagcagacgt ggccgcgatg atccatgaaa tagcggcagc
     1381 cgcagaggaa caatctgctt ccgttgaaga ggtgacagcc actgtagagg atgtgtcggc
     1441 aatatgtgaa caatcagcgg caggcacaca ggaaacatca gcagcggccc aggagcaggc
     1501 agcttcaatg gatcagctct ttaatgcagc ccaggaactt gccagattat caaatgaatt
     1561 gcagacagaa atctcaaagt tcaatatagg tgaaagtgta agatgaagac aaaattactg
     1621 gatatcctta cattttcaga aaaaagagag aatttgctca tattattgag agaagggcca
     1681 aaatcacttg aggaaataag atcatctctt aatgttacat cttccggaat gataccccag
     1741 ataaggaaac tcgaagaacg gaaccttgtt catagaattg ggaaaaatta cgagcttacc
     1801 gatataggga tgattgttac caatgctttt ttccctctta taaagaccat ggaatttata
     1861 gaaaaacatg agaatttctt gaaggaacat gatataaaag caattcctgt ccacctttta
     1921 acgagaatat tcgagtttga aaattcaagc cttaccgaaa gcaagataag tgaattatat
     1981 gaaccgcata aggaatttat gaaatatctt cgggattcca gaaaattcct gggaatagcg
     2041 ctggtattcc accattcata tcccccatta ttccttaagc tggcagaaag cgggaggcag
     2101 gtttcgatca ttctcacaaa agaggtgttt gagaaagtaa aaaaagaata tgaggttgag
     2161 ctttcaaaat tcattggact ggaaaacgga caattattca tatccaatga aataataaga
     2221 ttggcaagtg cggtcaccga taattttatg tctttgtcac ttttctttaa gagtggtggc
     2281 tacgattccc agagggagct tgtaagctcg gataaaacag caataaaatg gggtgaggaa
     2341 ttgttttatc attttattgc acattcaaaa aaagttaaaa atctgaaagc tctttaagaa
     2401 caactgcaag catgaattct ccattgacaa gggtgttgcg aagcgatttc ctgatccagt
     2461 caccattttc gcaattcggt cttgtccaac aaatggtatt aataataccc gcgaacgaag
     2521 atataagtag tatagctatt tccgggatgt aactgtattt aaaaatgata tttctgccag
     2581 cgagttatcc cagaatgtaa aagatgatat caacgctaaa ctccttgttt attcttcagc
     2641 ttttgataag atagaagcga tcaataagga gatgacgtca aaagaaaatg agttcaggga
     2701 taaagtgcat caaatagagc cattgacaac tggattattg gaaaatgcgc aaaccgataa
     2761 agccgccatg atcgcgcaat cagattcaaa taattccaat gcaagaaaca gtgttgtaat
     2821 actttccata atagcggtgt taacctgtat tactatcggc atcattattt cccgtttctt
     2881 aaggaagttc gcaggaaaag ttcaggatgc tgtgttaaag gtaggcgcaa cggctgaaga
     2941 actctcagca tcgagccagg aaatgaaagc cttctccgaa cagatatccg ttacaacaca
     3001 ggacattgca accggggctg gacaacaatc aatgaagatg gccgatgtca gcaggaccat
     3061 gagggagatg tccatgagcg ttcaacaggt tgcaggaaat gcaacaaagg ctgctgatag
     3121 tgcggataaa gctcatataa ctgcaaaaga gattgggcaa aaatcccagg aggttgtcaa
     3181 gcagatgacc gagatcagaa gtgcggtcga taattcatca atagtgataa aacagcttga
     3241 cggcaaatca cag
//
LOCUS       DATGBN010000216         4668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_482440, whole genome shotgun sequence.
ACCESSION   DATGBN010000216 DATGBN010000000
VERSION     DATGBN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482440"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VKL21_05200"
     CDS             complement(<1..556)
                     /locus_tag="VKL21_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMB45198.1"
                     /translation="MRTEKSRKLFEEAKKILPGGVSSPVRAIKPYPFYTKKANGSKIT
                     DIDGNEYIDYVMGYGPLLLGHHHPSIKEAVTRQLDDGWLYGTPTQLEVALAKEIIELY
                     ASIDMVRFVSTGTEATMGALRAARGFTGKNKIIKIEGGFHGAHDAVLVKAGSGATTLG
                     TPDSAGVPKDFTKNTLQVPYNDIDA"
     gene            complement(599..1300)
                     /gene="ubiE"
                     /locus_tag="VKL21_05205"
     CDS             complement(599..1300)
                     /gene="ubiE"
                     /locus_tag="VKL21_05205"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HMB45199.1"
                     /translation="MSQLQKKEYVRNMFAGISKRYDFLNHLLSLEKDKKWRRYAVSKL
                     PSGYIIDVCSGTGDVAIEAAKKNRVIASDFCFEMLKLCKPKLEKKNLKNVSCIRNDAE
                     NLSFKDGTFDGAIVAFGIRNVADIKKAISEMNRVVRIGGRVVILEFSLPENEVFRSMY
                     YFYFKKILPLIGAVISEKNGPYEYLPASVMAFPKRNEFVEIMKDAGISHVEYFDLSFG
                     IVTVYVGDRIDGIIV"
     gene            1545..2174
                     /locus_tag="VKL21_05210"
     CDS             1545..2174
                     /locus_tag="VKL21_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HMB45200.1"
                     /translation="MTIVENSPDKKPEVMRNILNISKKLAKENQGALFIIADRKQIEG
                     NYRLHYPQIQFAGNLLSKGMNAVVEKLATLDGAIIFTPDGEMVAYGSRILKSETLLGF
                     GTKHAAARGITLYDDTITAILVSEESGWIKVFQKGEIVLETDSVDIEPSTLDKVSRFL
                     TNQDTALLASAGIAAAALGPQIVLIVGGAYMMVRTAGQMISSALKKEKK"
     gene            2175..2987
                     /gene="dapF"
                     /locus_tag="VKL21_05215"
     CDS             2175..2987
                     /gene="dapF"
                     /locus_tag="VKL21_05215"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMB45201.1"
                     /translation="MVHFTKLHGNGNDFILIDEYNEQIIDDEKKAEFTLKYCDRRFGI
                     GADGVLFLGRSNIAHIRMRIYNSDGTQAEMCGNGVRCLVKYALDKGYIKEKGSVETLA
                     GVLPISSKKGDKTWICVNMGKPQFQRTKIPAKGTGEFLNVAMHGYVVSAINTGVPHAV
                     VFVDSLDDPELMQEAPKIRNDGIFPKGTNVNFVKVNSGDEITIRTYERGVEGETLSCG
                     TGSVACAAIAHRLGMTGNNVKVNTKGGELRITLTDDAAYMEGQAQRVFEGNI"
     gene            3328..3597
                     /locus_tag="VKL21_05220"
     CDS             3328..3597
                     /locus_tag="VKL21_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45202.1"
                     /translation="MIKDKILMKILSGVNDANIRFQDLRNFILRYDFSERVRGDHYIF
                     SKENVFEIINLQPLKDGKAKPYQVKQIRKLFLKYKFHLNEENYER"
     gene            3587..3805
                     /locus_tag="VKL21_05225"
     CDS             3587..3805
                     /locus_tag="VKL21_05225"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HMB45203.1"
                     /translation="MKDEIKYEMVIYWSEEDDAYIAEVPELPGCMADGRTYEGAIKNT
                     LTVVKEWIETANELGREIPKPKGRLIYA"
BASE COUNT         1476 a          841 c          949 g         1402 t
ORIGIN      
        1 tggcatcaat atcgttatac gggacctgga gtgtgttttt tgtgaaatcc ttcggaacac
       61 ccgccgaatc aggcgttccc agggtcgttg cgccggaacc cgcttttaca agcacggcat
      121 catgtgctcc atggaagcct ccttcgatct tgatgatctt gttcttgcct gtgaaacctc
      181 ttgcagcgcg aagcgctccc attgttgctt ctgttcctgt ggatacaaat ctcaccatgt
      241 cgatactggc ataaagttca attatttctt ttgccagagc gacttcaagt tgggtcgggg
      301 tcccataaag ccaaccgtca tcaagctgcc tggtcacagc ttcctttata gaaggatgat
      361 gatggccaag aagcaaaggc ccataaccca taacgtaatc gatatattca tttccgtcta
      421 tatcggttat ctttgagccg ttggcttttt ttgtgtaaaa cggatatggt tttatagctc
      481 ggaccgggct gctcacgccc cccggaagga ttttctttgc ctcttcaaat aattttcttg
      541 atttttctgt tctcatggat tactgaaatt aggggataaa gcaatataat ttttggcttt
      601 acacgataat cccatcaata cgatctccaa cataaactgt aacgatcccg aaagacaggt
      661 caaaatattc aacatgcgag ataccagcat ctttcataat ttcaacaaac tcattccttt
      721 tgggaaacgc catcactgat gcgggaagat attcgtaagg tccgttcttt tcggatatga
      781 ccgctccaat taacggtaag atctttttga aatagaaata atacatggac ctgaaaacct
      841 cattctcagg cagggaaaat tcaagaatta ccaccctgcc tcctatcctg accacccggt
      901 tcatctcgga tatggctttt tttatatctg cgacatttcg tattccaaaa gctacaatag
      961 ctccatcaaa agtaccatcc ttaaaagaaa gattctcggc atcgttccgg atgcaactta
     1021 catttttcaa attctttttc tctaacttgg gtttgcataa tttaagcatc tcaaaacaaa
     1081 aatcagaagc tatgacccga ttcttttttg cggcttctat tgcgacatcc cccgtgcccg
     1141 agcagacgtc aataatatat cctgatggca gttttgatac tgcataacga cgccattttt
     1201 tatccttttc aaggctcaac aggtgattca gaaaatcata tctttttgaa atacctgcga
     1261 acatattccg gacatattct tttttctgca attgagacat tgttatctgc tgttccttaa
     1321 ggaaggggat gaatcttact tcctccaatt gtaatatcta tcccaggaaa acagttgtag
     1381 aggctgtatt ttagggatgg tcataagaat agtatcaaaa tgatatatag tttgtcgaag
     1441 tttcatgaag aaagcattaa cgaaaactgg aacgacaagc tcctgccctt taggggaagt
     1501 tgttgaccaa aacttataac ttatcctgat aataagtagg ttctatgacc attgtagaga
     1561 atagccctga taagaaacct gaagtaatgc gtaatatcct taatatttca aagaagcttg
     1621 ctaaagaaaa tcagggagca cttttcatta ttgcagacag gaaacaaatt gaaggaaatt
     1681 accgccttca ttatcctcag atacaatttg caggaaattt gctcagcaag ggcatgaatg
     1741 ctgtggtgga gaaacttgct acgcttgatg gcgctattat atttaccccc gatggcgaaa
     1801 tggttgcata cggttcgagg atattgaaat ctgaaacact ccttggtttt ggaactaaac
     1861 acgcagctgc cagaggtata acattatatg acgacactat aactgctatc cttgtctctg
     1921 aagaaagtgg atggataaaa gtattccaga aaggagagat cgtccttgag actgattcgg
     1981 tggatatcga accttccaca cttgataaag tttcccgttt ccttacgaac caggatacgg
     2041 cgcttctcgc aagcgccggt attgctgcag ctgctttagg tcctcaaata gtattgatag
     2101 ttggaggagc atatatgatg gtaaggactg cagggcagat gatatcctct gcattaaaga
     2161 aagagaagaa gtaaatggtg cattttacga aacttcacgg taatggaaat gatttcattc
     2221 tcatagatga atataacgaa caaatcattg atgacgaaaa aaaggctgaa tttacattaa
     2281 agtattgtga ccgacgcttc ggaataggag cagacggtgt gcttttcctt ggaagatcaa
     2341 atatagccca catcaggatg agaatatata attccgatgg cacacaggcc gagatgtgcg
     2401 gaaatggtgt gcgatgtctt gtaaagtatg cactcgataa aggatatata aaggagaagg
     2461 gaagtgtgga aacgcttgca ggcgttctcc ccataagttc gaagaagggc gataaaacct
     2521 ggatttgcgt aaatatggga aaaccgcaat tccagaggac aaaaatccct gcaaaaggaa
     2581 caggggaatt tctaaatgta gctatgcatg gatatgtagt atcggcaata aacacaggtg
     2641 ttcctcatgc agtggtcttt gtagattcac ttgatgaccc ggaattaatg caggaagcgc
     2701 caaagatacg aaatgatggg atttttccaa agggaacgaa tgtgaatttc gtgaaggtga
     2761 attccgggga tgagataacg atccgcactt atgagcgggg tgtggaaggt gaaacattaa
     2821 gctgcggaac tggttcggta gcctgcgctg caattgctca ccggctcgga atgaccggaa
     2881 ataatgttaa agtgaataca aaaggtggcg aattaagaat tacacttacc gatgatgccg
     2941 catatatgga aggacaggct caacgggtat ttgaagggaa tatttagaac gtggggtaga
     3001 gaaccccacc acattgggtg tttaagaatt tcatactctt tggcataggt tattgtatgc
     3061 atctggtcct caaggaaagt tttgactctg gtatcttcaa gtattagttt tttggttata
     3121 aaaggggtag atgagtggat aaaagcacaa atttaataga aaatgtattt aatcaatcaa
     3181 atgtatcatg attaaatctg gtggctactt tggaaaaaaa ggatattgat tttttacaga
     3241 aaatcataaa ttctggggcg caggataacg atgttggggg aatatacaac ggatgaaaat
     3301 tgtgaatcat taagaggttt ctgaagcatg atcaaagata aaattttgat gaaaattcta
     3361 tcgggtgtta atgatgcgaa tattagattt caggatttaa gaaattttat attacgttat
     3421 gatttttccg agagggtacg aggtgatcac tatatatttt cgaaagaaaa tgtttttgaa
     3481 ataataaatt tacagccact taaggatgga aaagcaaaac catatcaggt taaacagata
     3541 agaaaattat ttcttaaata taaattccat ttaaatgagg aaaactatga aagatgagat
     3601 aaaatatgaa atggtaatat actggagtga agaagacgat gcatatattg cggaagtgcc
     3661 agaacttccg ggatgcatgg cagacggcag gacttacgag ggggcaatta aaaatactct
     3721 aactgtggta aaagagtgga tagaaactgc aaatgaactt ggcagagaga taccaaaacc
     3781 aaaaggaagg ttgatttacg cttaattgtt gaaaaacaaa tgctaaccaa tatagaatca
     3841 ccgtctcggg atattgcaat attattccat tgcaacatta tgatggttta aattatatcc
     3901 catttttttt aatcttttat aagtttcaaa tccttttttg ccatatgaaa cgaaccaatc
     3961 tccatctttc attaaattcg tactgaatcc actttcataa aaaattattc caccattaga
     4021 ttcatatgct cgatcaaata attgtctcat caaaggagaa ttttcaatct cttttttttc
     4081 gcctttcgtg tcgttctcgt taaagacaaa ttataggatg tttaacaaaa aatttgggtt
     4141 gaatctgaat tggttttact ggtttaatat aatgcaaatc tggattccat acccttatta
     4201 tattaatatt agtgggatgc tctgatgtga gacttaaaac aatattccac agatcgtttt
     4261 ctactaagaa cttatgcgac ttctccagtt gacggtctat cgttcttaaa atcatatccg
     4321 cagcagatat gtagcaatta caattatcac cttttgctac ggtatttact ttattgtatg
     4381 taacaagctc atgccatgct cgtgattctt ctccttcaaa gaaatccact aagaaatatt
     4441 gccctttcat ttttgttata tctgtgagtt tccatgcgca gactattgga taataatttt
     4501 ctatcctttg aatccattac atggaatcaa atatttaaat ccaaatattt gagttaacta
     4561 tatctcattt caagacgtaa caatgaagtt taaatatgac agatgagaaa tttttgataa
     4621 cagaactggt attgacagcg catctgtata atgagtcaga taagctta
//
LOCUS       DATGBN010000217         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_55523, whole genome shotgun sequence.
ACCESSION   DATGBN010000217 DATGBN010000000
VERSION     DATGBN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55523"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /gene="trpA"
                     /locus_tag="VKL21_05230"
     CDS             complement(<1..754)
                     /gene="trpA"
                     /locus_tag="VKL21_05230"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HMB45204.1"
                     /translation="MRISEKFRQLKENNEGALIAYICAGDPTPAATKEYVTALVRGGA
                     DIIELGLPFSDPVADGPTIQAGIQRALDGGMTPDIYFRTIASLKLHIPLVVMTYYNLI
                     FKRGLTKFTKDCATSGISGIIVPDLPPEESDELLQACKENDIDLIYLVAPTTTQERME
                     WILAEGTGFIYLVARLGVTGARSDVAGSTGELIKRVKTATPKAVGFGISNGKQAARII
                     RAGADGVIVGSAFVDIIAKGEDVTAKLEALARE"
     gene            complement(751..1938)
                     /gene="trpB"
                     /locus_tag="VKL21_05235"
     CDS             complement(751..1938)
                     /gene="trpB"
                     /locus_tag="VKL21_05235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HMB45205.1"
                     /translation="MKNKLESTKFGKYGGQFVPEVLMPALEELTEYYEKFRDDAVFKE
                     ELDYYLRDFAGRPSPLYFAKRLSKEYGVKIYLKREDLVHGGAHKLNNTLGQGLLAKYM
                     GKKRIIAETGAGQHGTACAMAGATFGINTEVYMGAKDTVRQRMNVYRMELMGTKVIPV
                     KSGSQTLKDAINEAMRDWVANVETTHYMIGSVVGPHPFPTMVRDFQSVIGKEVRSQIM
                     EKEGRFPDSIVACTGGGSNAMGIFYPFIEEDVDLFAVEAGGSGMKTTDKEALHSATLC
                     AGEDGVLHGMRTKVLQNKYGQILESSSIAAGLDYAGVGPELAYLADIGRLKPCNSMDS
                     EALLAFHDLCRLEGIIPALESSHAVAYVKKAAATGELGEIVVINISGRGDKDLETVMG
                     MRK"
     gene            complement(1962..2732)
                     /locus_tag="VKL21_05240"
     CDS             complement(1962..2732)
                     /locus_tag="VKL21_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HMB45206.1"
                     /translation="MHQIIDEIIRSTKDRIPSASKIEKRKITSRHFKQSIEDKKQKDL
                     IPVISEVKPSSPTRFIRNVTPHNASEIAIQMESAGAAAISVLTEPFYFKGSMQNLISV
                     REAVKIPVLRKDFIINKNQIHESDSDLILLIAGILGDELDDFVNEAISSGREPLVEVH
                     DSKELENALLSRTNIIGINNRNLTTMKVDITTTENLAPLISNRIIISESGIFSPLDAV
                     RMIDAGADAILAGTSIMLGNVYEKTYELVHALNAKKGN"
     gene            2873..>3414
                     /locus_tag="VKL21_05245"
     CDS             2873..>3414
                     /locus_tag="VKL21_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB45207.1"
                     /translation="MNGKNNIPVSQPLVSIIIPCMNEEKTIGVCIKKALSTLEREGYE
                     GEIIISDNSTDTSRDIAMKLGVKVVIPYNKGYGNAFLEGFRHAKGKYVLLADADDTYD
                     LTEIRKFLEPLMADKADFVMGTRLRGHIEKGSMPWLHRYIGNPIITSTLNDLFKTHLS
                     DSQCGMRSIVRKEYDKLDLK"
BASE COUNT          880 a          805 c          703 g         1026 t
ORIGIN      
        1 attcccgcgc aagcgcctca agtttcgcag ttacatcttc acctttggca ataatatcca
       61 cgaaggccga cccaacgatc actccatccg ctcccgcccg gattatccgg gcagcctgct
      121 tgccgtttga tatcccgaat cccacggctt tgggtgtggc agtctttact cttttaataa
      181 gctcacccgt cgaacctgct acatctgagc gcgcgcctgt aacacccagc cgcgccacaa
      241 gatagataaa gcctgttcct tctgccagta tccactccat tctctcttgc gtggttgtgg
      301 gagccacaag ataaataaga tctatatcgt tctctttgca tgcctgcagg agttcatcgg
      361 attcctcagg gggaaggtcc ggaactatta taccggatat tccggatgta gcgcagtctt
      421 ttgtaaattt tgtaagtcca cgtttaaata tgagattata atatgtcatc acgaccagag
      481 gaatatgcag tttaagcgaa gcgattgtcc tgaaataaat atcaggcgtc atccccccgt
      541 caagcgccct ttgtatacct gcctggatag tgggaccgtc agcaaccggg tctgagaatg
      601 gaagaccaag ttcgataata tcggcgcccc ctcgtacaag cgccgtaaca tactcttttg
      661 tagcagcagg cgtggggtcg cccgcacaaa tataagctat cagcgcgccc tcgttatttt
      721 ccttaagctg cctgaatttt tcgcttatcc tcattttctc atccccataa cggtctcaag
      781 gtctttgtcg cctcttcccg aaatgttgat aaccacaatt tctccaagct cacctgtcgc
      841 tgccgctttt ttgacataag ccaccgcatg cgaagattca agcgctggga ttatgccttc
      901 cagcctgcaa aggtcatgga acgcgagaag tgcctcgctg tccattgaat tacatggttt
      961 gagacgcccg atgtcagcca gatatgcaag ttccgggccc acgcctgcgt aatcaagacc
     1021 agcagcaatt gaacttgatt caagtatctg cccgtactta ttctggagca cttttgttcg
     1081 catgccgtga agcactccgt cttcacctgc gcaaagggtt gctgaatgaa gcgcttcttt
     1141 atcagtagtt ttcatgccgc tacctcctgc ttctactgcg aacaggtcaa catcctcttc
     1201 tatgaacgga taaaaaatac ccatagcatt gctgcctccg cctgtgcatg cgactatgga
     1261 atcaggaaaa cgtccttcct tttccattat ctggcttcgt acttcttttc cgatcacgct
     1321 ctggaaatca cgtaccatgg tcgggaaagg atgcggcccg actaccgagc ctatcatata
     1381 atgagttgtc tcgacatttg ccacccagtc acgcattgct tcgttaatgg cgtcctttag
     1441 tgtctgagac ccgcttttta cagggatgac ctttgttccc atcaattcca tcctgtaaac
     1501 gttcatgcgc tggcgcactg tgtcctttgc gcccatataa acctcggtat tgatcccaaa
     1561 agttgcgcca gccatggcac atgccgtacc atgctggccc gcgcctgtct cagcgataat
     1621 tcttttcttt cccatatatt ttgccaggag cccctggcca agcgtattat tcaacttatg
     1681 ggcgccgccg tggacaagat cttcacgttt taagtagatc ttaacaccgt attccttgct
     1741 caatcttttt gcaaaatata atggggaagg gcgccccgca aaatcccgca gataataatc
     1801 cagttcctct ttgaaaactg cgtcatccct gaatttttca taatattcag taagctcttc
     1861 aagggccggc atcaatactt caggtacgaa ctgaccccca tatttgccga attttgtgga
     1921 ctcaagctta tttttcatat ttgtaatcct tttagtgtta tttaatttcc ttttttagcg
     1981 ttcaatgcat gtactaattc gtaggtcttt tcgtaaacgt ttccaagcat tattgatgtt
     2041 cctgcaagta tcgcatctgc ccctgcatct atcattcgta cagcatccag aggtgaaaat
     2101 attccgcttt cacttattat tatccggttg ctgataagag gggccagatt ttcggtagtg
     2161 gtaatatcaa ccttcatggt agtaaggttc ctgttattta tcccgataat atttgtcctg
     2221 gacaagaggg cattttcaag ttccttacta tcatgcacct caaccagagg ctcccttccg
     2281 gatgagatgg cttcatttac aaaatcatcc agttcatcac cgagaattcc tgcgatcaga
     2341 aggatcaaat cgctatcgct ttcatgaatc tggttcttat taataatgaa atcctttcga
     2401 aggacgggta tttttaccgc ctccctcaca gatatcaggt tctgcatact tcctttaaaa
     2461 taaaatggtt ccgtaagcac agatattgct gccgcacctg ctgattccat ctgaattgca
     2521 atttcagagg cgttatgggg tgtgacattt ctgataaatc gtgtaggtga ggatggtttg
     2581 acttctgata tcacaggaat aagatctttc tgtttcttat cttcgatgct ctgtttgaaa
     2641 tgccgtgagg taattttcct tttttctatt tttgaggcag aagggatcct gtcttttgtt
     2701 gatcttatta tttcgtcgat tatctggtgc ataagtatcc atcatattgt acagtttagt
     2761 acattgatgt acaattatgt tcatcatatt taaagatttt ggattgttta gatacatata
     2821 gagatatact agcgatagtt ttacgttcaa ataatatctt ttacaatacc agatgaatgg
     2881 caaaaataat atcccggtga gccaacccct tgtatcaatc ataatcccat gcatgaatga
     2941 ggaaaagaca ataggagtgt gcataaaaaa agcattgtca acattggaac gggaaggtta
     3001 cgaaggggaa ataataatat ctgataattc tactgacact tcaagggata tcgccatgaa
     3061 actcggggta aaagttgtaa taccgtataa taaaggatat ggcaatgctt tcctggaagg
     3121 attccggcat gcaaaaggaa aatatgtttt gctggcagat gcagatgata cttacgatct
     3181 tacggaaatt agaaagtttt tggaaccttt gatggctgac aaggcggatt ttgtaatggg
     3241 tacaaggtta aggggccata tcgaaaaggg ttcaatgcca tggcttcaca ggtacattgg
     3301 aaatccaata ataacaagca ctcttaatga tcttttcaag acccatctta gcgattccca
     3361 gtgcggtatg agatcgattg tcaggaaaga gtatgataag cttgatctta aaag
//
LOCUS       DATGBN010000218         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_249854, whole genome shotgun sequence.
ACCESSION   DATGBN010000218 DATGBN010000000
VERSION     DATGBN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249854"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VKL21_05250"
     CDS             <1..529
                     /locus_tag="VKL21_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45208.1"
                     /translation="IIRLKDPARANGVQEELMQQYPQYTISTNQDYLNKVIKQNALPL
                     ACAGSIVILAVIMGTMLAVNTMLLSLNEKKKEIAILQVIGLSRWSIFKSIGTEGLLIS
                     LLGGAIGVLISIPIVPVLNDLIHAYIGFDKLVILQNDYLYMGFAIAVIIGLLTSFLAS
                     MKTSRMNTAGLLRGV"
     gene            complement(703..870)
                     /locus_tag="VKL21_05255"
     CDS             complement(703..870)
                     /locus_tag="VKL21_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45209.1"
                     /translation="MCSTYSGFSMMPDDLEEMKKGSSMFWCVSCKKTFMKKGATECPN
                     CGSNKIKVRAP"
     gene            complement(1097..1357)
                     /locus_tag="VKL21_05260"
     CDS             complement(1097..1357)
                     /locus_tag="VKL21_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HMB45210.1"
                     /translation="MAETDNSQFNNRDYQEIAILALDLLQICMVYIYTLLVQKVLSEK
                     EWLDKMQPEDFRVLTPLFYGHITPYGEFLLDMNKRINIKGTI"
     gene            1579..2499
                     /locus_tag="VKL21_05265"
     CDS             1579..2499
                     /locus_tag="VKL21_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45211.1"
                     /translation="MKKVLGVLFILILLTAVISYAEENKGAKDANAVHTEKGNQVTAF
                     ENLYQSFMKSSLPHSLDKFFKTQQPEVPGNEWLGEMFTLSGTFEGIATNLQDGDRVNA
                     TLSYNAFAKEYKNVYKNVSKKVPEWEGYFDIKTVDKLGKDLKSNNDDASFQDIGIIGN
                     TCSRCHADRQPQVWAKYYWRDFNTVNVTTDTGNLSWTPAMFALAGAFDGIGVNAAKGN
                     QTATNKSFIQFKALYTDIKTACSNCHDTPRYYYVSDDVFAMINQTGDFIQAGDLKNAS
                     INQQILGIQCYKCHVLHMPAQDMKAKMETK"
BASE COUNT          848 a          521 c          531 g          833 t
ORIGIN      
        1 aattatccgt ctcaaagatc cagccagagc aaacggggtt caagaagagc tgatgcaaca
       61 gtacccccag tacaccatca gcacgaacca ggattacctg aataaagtta taaaacaaaa
      121 cgctcttccc ttggcctgcg caggatctat cgttattcta gctgtcataa tgggaactat
      181 gttggcagta aacaccatgc tactttcact aaacgagaaa aaaaaggaga tagcgatcct
      241 tcaggttata ggcctgagtc gctggtctat atttaagagt ataggaacag aaggcctcct
      301 gataagcctt ctcggtggcg ctataggggt gcttatcagc atacccatag ttccggtgct
      361 taatgacctg atccatgcat acatcgggtt tgacaaattg gtgatattac agaatgatta
      421 cctttacatg ggatttgcca ttgcggtcat catagggctt ctgacaagtt ttcttgcctc
      481 gatgaagaca agcaggatga acactgctgg attattgaga ggggtatgac accttcgccc
      541 taagggtatc accacgattc gtccgtaaaa tgtccataag acagtaatta aataattacc
      601 taccaaaata tttattaatt cggtatacac tcagggatat gtcatgggga tgaaatacat
      661 tacggttata attcccggca atatttattt taattattta aactatggcg ctcttacctt
      721 gatcttattt gagccgcagt tcgggcattc tgttgctcct ttcttcatga aagtcttctt
      781 gcatgaaacg caccagaaca tggagcttcc tttcttcatc tcttcaaggt catcaggcat
      841 cattgaaaat cctgaatatg tactgcacat aacaacctcc atttactatt cttgtattat
      901 ggcaatttat ggtttgttgc tttcgatatt aaaggttttg gttgaggttt tatctgtttt
      961 ttcgggatat ccctttatac tttcacttgg ctctctttga tatcactatc tgataatttt
     1021 gaaacctgag ctaaactgtt cctgtgtaaa aggggtagta ccagcgccgc tgcaaccaag
     1081 tgccactttt gttgatttag attgttcctt taatatttat tcgtttattc atatctaaca
     1141 ggaactctcc atatggcgtg atgtgtccat aaaaaagcgg tgtcaagacc cgaaaatctt
     1201 ccggctgcat tttgtccaac cactctttct cagataacac tttttgcacc aaaagtgtat
     1261 aaatatagac catacaaatt tgcaacaggt ccaaagctaa aatagctatt tcctgatagt
     1321 ctctgttgtt aaactgcgaa ttgtccgtct ctgccatacg attgaagaag ttgaaaattc
     1381 atagaatggg tttgcatggc acttggttgc agcggcgctg gtactacccc agaaagacca
     1441 aattcgtcta atattcgcta atattagcga gattattata tttaccatta tcacaacttg
     1501 ataatggtaa tataactatt aatagtgtag agaataccat taaatctggg tgttttatcc
     1561 catctttgga ggtatgaaat gaaaaaagta ttaggtgtat tatttatttt gatattgcta
     1621 acagcagtaa tatcatatgc agaagaaaat aaaggagcta aagacgcaaa tgctgtacat
     1681 acagaaaaag gtaatcaggt tactgcgttt gaaaatctgt atcagtcgtt tatgaaatcg
     1741 tctcttccac attccttgga taaattcttt aaaacacaac aacctgaagt tcccgggaac
     1801 gagtggctag gtgaaatgtt cacattgtca ggtacatttg aaggaattgc aacaaatctt
     1861 caagatggcg atagagttaa tgctacttta tcttacaatg cgttcgcaaa agaatataag
     1921 aacgtatata agaacgtatc aaagaaggtt cctgaatggg agggatattt tgatataaaa
     1981 acagttgata aacttggaaa agacctcaaa tctaataatg atgatgcttc attccaagat
     2041 ataggaatta ttggtaatac ctgttcaaga tgtcatgcag atagacagcc tcaagtatgg
     2101 gcaaaatatt actggagaga ctttaataca gttaatgtca ctacagatac cggaaatctt
     2161 tcttggacac ctgcaatgtt cgctctggca ggagcatttg atggcatagg agtaaatgct
     2221 gccaaaggca accagactgc gacaaacaaa agcttcatcc agttcaaggc tttatatact
     2281 gatatcaaga ccgcatgtag caattgtcat gataccccaa gatactatta tgttagcgat
     2341 gatgtatttg ccatgataaa tcagacaggg gatttcatac aggcggggga tttaaaaaat
     2401 gcttcgataa atcagcagat acttggaata cagtgctata agtgccatgt tttacacatg
     2461 ccagcccagg acatgaaagc taagatggag acgaaataaa cctctccatt tcaatttttt
     2521 gtgttaaatg taggacgtat atcccgggtg gaggtacgaa agaaccgtct ttcttggtga
     2581 atatatcatt gcgaccatcc ccccatacta ttgtaatgtt tccatgatct tatgcatgac
     2641 actgttccaa aatccagtga ttttgacaag catcacatat gcatttttta tggatttaca
     2701 ccgatgctat tttaaattcg tgcaaatcaa tgt
//
LOCUS       DATGBN010000219         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_560298, whole genome shotgun sequence.
ACCESSION   DATGBN010000219 DATGBN010000000
VERSION     DATGBN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560298"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VKL21_05270"
     CDS             <1..366
                     /locus_tag="VKL21_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HMB45212.1"
                     /translation="TLFILYENRLAGYRRERDSDSGWLTYLWKLDLSNLNRQLELEAK
                     KLLKNLKTKLEYEEDKVFYICKNLDGRFLFEIAAEMEFKCPFCGNELIFEENEPVIEA
                     LKQRIVQLEDLTSSSGAVA"
     gene            363..890
                     /locus_tag="VKL21_05275"
     CDS             363..890
                     /locus_tag="VKL21_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00295 family protein"
                     /protein_id="HMB45213.1"
                     /translation="MNEKDAIAFLISSGCSPDLIQHCKAVAEYAKEIAINIRENAFLK
                     GDPIHIDVDAVFVGALLHDIGRSKTHGLSHAIEGARIAMDNSLDDKIVKIIERHIGAG
                     IPKEEASVLGLPEKDYLPVTIEEKIVAHADNLVFGKEIGTMDELISNLRRKKLDETTI
                     LRFIELNNEISSMMA"
     gene            complement(877..1197)
                     /locus_tag="VKL21_05280"
     CDS             complement(877..1197)
                     /locus_tag="VKL21_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HMB45214.1"
                     /translation="MDSLKVRIKRRLEVYLELDIDGIRKFIIDILLRLKKVTVDQLFN
                     ELNKKFKISFSAVASTLGYIHSKLGILHAYKESYKTPIVYSLKEEYVDIVENALSKSA
                     AKPS"
     gene            1502..2785
                     /locus_tag="VKL21_05285"
     CDS             1502..2785
                     /locus_tag="VKL21_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45215.1"
                     /translation="MAYQFVDREKELSFLKEKYSSKTAELIIIYGRRRVGKTELIKQS
                     ISKSPLKSLYLLGELQKENQLSSIYSNIAGISLDDDFLKNSPLNTWQAFFDYLTRYID
                     KNGMVLVIDELPYIHKTNPNFISILQFFWDEKWKNMNLKIILCGSSISMMQKIALSYS
                     SPIYGRRTGQIDLQPLTYLDFRSLFNEWDEEDILGAYAVLGGIPRYAEEFDKKKSLPE
                     NICRAFLDKDAFLYKEAKFLLMEELQDFANYFSILKAVAFGKNTFNEISNFSGVSSNK
                     LFVYISKLIELNIIRRDIPVTLSKEKSTRVGNYVLKDYFFRFWFRYIYPNSSLIEIGK
                     PEIVLDIIKRDFNNYLDTIFEDISTELIRNLSLNGKLPLFTKWGKWWRMDYEIDIVAT
                     NEVTGDILFCECKWEDKQTDRPVLEELMEKSEKVE"
     gene            complement(2925..>3557)
                     /locus_tag="VKL21_05290"
     CDS             complement(2925..>3557)
                     /locus_tag="VKL21_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMB45216.1"
                     /translation="IIKGEKPEREERDVATFMNAMASKPDKVVEEAVDHCLDRKKNAK
                     KVLDLGGGPGKYAKAFVNRGLNAVLYDLPDTIDYVSARFGLGNIKNLTLKKGDFTNEF
                     ADEFAGESFDIIFMGNICHIYSEKENRMLIKRAGNILKSGGMIAIEDMVRGRSPMAEM
                     FAVNMLANTENGSTYTEPQFREWLTDAGFAKIEIADIAQKNNQLITAFLD"
BASE COUNT         1079 a          696 c          711 g         1071 t
ORIGIN      
        1 actcttttca tattatatga gaacaggctt gctggttatc gaagagaaag ggattcagac
       61 agcggttggt tgacatatct ctggaaactt gaccttagta atttgaatcg ccagcttgag
      121 cttgaagcca agaagctctt aaagaacctc aaaacaaaac tcgaatatga agaagataaa
      181 gtattttata tatgtaaaaa cctggatggc aggtttttat ttgaaatcgc ggctgaaatg
      241 gaattcaaat gcccattttg cggtaacgaa ttaatattcg aggaaaatga gcccgtaata
      301 gaagccttaa aacaaagaat cgtccaactt gaagatttga catcctcttc aggtgcagtt
      361 gcttgaatga aaaagatgcg atcgctttcc tgatatcttc gggatgctct cctgatttga
      421 tccaacattg taaagccgtt gcggaatatg caaaggagat cgcaataaat attcgcgaga
      481 atgcattcct gaaaggagat ccgatacata tcgatgttga tgcggttttt gtgggagctt
      541 tgctccatga tatcggacgg tcaaagactc atggcttaag ccatgcaata gaaggcgcca
      601 ggatagcaat ggataatagt cttgatgata agatcgtaaa gatcatcgaa cggcatatag
      661 gtgcaggaat cccaaaagaa gaagcttccg tcctcggcct tcctgaaaag gattacctgc
      721 ctgtaactat tgaagagaaa attgttgcac atgccgacaa tcttgtcttt ggcaaagaga
      781 tcgggactat ggatgaatta atctcaaatc ttagacgcaa aaagctggat gaaacaacga
      841 tcctgcggtt tattgaatta aacaatgaga tatcatctat gatggcttag ctgcactttt
      901 gcttaatgcg ttctccacaa tatccacgta ctcttccttt aatgaataga ctatgggggt
      961 tttatatgat tccttgtaag catggagtat ccctagtttt gagtgaatat atcccaaagt
     1021 tgatgcaacg gcgctgaatg atatcttgaa ttttttgttc agttcgttaa acaattggtc
     1081 tacagtcact ttctttaatc gtaacaggat atcgattatg aatttcctga taccatctat
     1141 atcaagctca agatatacct caagcctcct ttttattctt acctttagtg agtccatcct
     1201 ttaatccctg gcctatacta aaaaacttct tttataatat atatacttta ctacttattt
     1261 gtagatatgt ccctcattaa ttacctttca acacataaaa tagaaagacg tgcgtccaat
     1321 atggatagga agagttattg aacgcacacc catgccaatt ctgtcatgct catgtaaata
     1381 tttttctatt tgctccggat agtgcgatag atccacatgt attaaatata ataattcaaa
     1441 ttatcataat ttactttatg ttaaacagaa gctttaatat atcacggatc aaatacagac
     1501 tatggcttat caatttgttg accgcgaaaa agaattgtct ttcctcaaag aaaaatactc
     1561 ttcaaagaca gctgaactga taataatcta cggaaggagg cgtgtaggta agaccgaatt
     1621 gataaaacaa tcaatatcaa aaagcccgtt aaaatccctg tacctgcttg gggaacttca
     1681 gaaagagaac cagctgtctt ccatatattc aaatattgcc ggaatttcgc ttgatgatga
     1741 tttcctgaag aacagccctt tgaatacctg gcaggcgttc tttgattatc ttacaagata
     1801 catcgataaa aatggaatgg tgcttgtgat cgatgagctt ccgtatatcc ataagacaaa
     1861 tccgaatttt atttcaatac tccaattttt ctgggatgaa aaatggaaga atatgaacct
     1921 caagataatc ctgtgcggct caagcatttc tatgatgcag aagatagcgc tttcctattc
     1981 gagcccgatc tatggcagac gcacaggaca gatagacctt caacccctaa cgtatcttga
     2041 tttcagaagc ctatttaatg aatgggatga agaggatatt ttgggcgctt atgcagtact
     2101 gggaggaata ccgcgttacg cagaagaatt cgataaaaag aaaagtttgc ccgaaaatat
     2161 ttgcagggca tttcttgata aggatgcttt cctgtataaa gaagccaagt tccttcttat
     2221 ggaggaactg caggattttg caaattattt ttccattctc aaggctgtag ctttcgggaa
     2281 aaatactttc aatgagatat ctaatttctc aggggtctcc tctaacaaac tatttgttta
     2341 catctccaaa ctgatcgaat tgaatattat ccggagggat atacctgtga cattgtcaaa
     2401 agaaaaatcc acacgggttg gcaattatgt cctcaaggat tatttcttca ggttctggtt
     2461 caggtatatt tatccaaact cctcccttat agagattggc aaacccgaaa ttgttcttga
     2521 tattataaaa agggatttca ataattacct ggataccatc tttgaggata tttcaaccga
     2581 gcttatcagg aatttatccc tgaatggaaa attgcctctt ttcacgaaat ggggtaaatg
     2641 gtggcgtatg gattatgaaa tagatatagt tgccacgaat gaggtgacag gcgacatact
     2701 tttttgcgag tgcaaatggg aggataaaca gacggatagg cctgttcttg aagaactgat
     2761 ggaaaagtct gaaaaagtag aatagagaga tggaaaacga aataaccatt atgctgtggt
     2821 gtcaaggaaa gggttcacgc aagatgcgaa aggatttgcg gataagaaaa agatacattt
     2881 attcacgctt ggggatattg ttcctcttta atgtcaaaaa aacgctaatc caaaaaagct
     2941 gtgataagct ggttattctt ctgcgcaatg tcagcaattt cgatcttcgc aaatccggca
     3001 tctgtgagcc attccctgaa ttggggttcg gtgtacgtgc ttccattttc agtgtttgcc
     3061 agcatattca cggcgaacat ttctgccatg gggcttcgtc cccggaccat atcttcgatg
     3121 gcgatcatcc cgccgctttt caatatatta ccggcgcgct ttatgagcat cctgttctct
     3181 ttttctgaat agatatggca gatattgccc atgaaaataa tatcaaatga ttcccctgcg
     3241 aattcatctg cgaactcatt cgtaaaatcc cctttcttca gggtcaggtt cttgatattc
     3301 ccgaggccga accgggcgct gacataatcg attgtatccg gcagatcata aagaaccgca
     3361 ttcaagcccc tgttgacaaa ggcctttgcg tacttgccgg gtccgcctcc caggtcaagc
     3421 actttctttg cgttcttctt tctatcaagg cagtgatcta cagcttcctc cacgacttta
     3481 tcaggctttg aagccattgc gttcataaaa gttgccacat ctcgctcttc cctttcaggt
     3541 ttttcccctt tgattat
//
LOCUS       DATGBN010000220         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_560350, whole genome shotgun sequence.
ACCESSION   DATGBN010000220 DATGBN010000000
VERSION     DATGBN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560350"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VKL21_05295"
     CDS             <1..200
                     /locus_tag="VKL21_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMB45217.1"
                     /translation="VLMVEDVATTGGSVLKAIELLRDEGLVVEHVIVVVDREEGALSA
                     LSKAGVELIPLAKVSELIPTK"
     gene            complement(1478..1804)
                     /locus_tag="VKL21_05300"
     CDS             complement(1478..1804)
                     /locus_tag="VKL21_05300"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HMB45218.1"
                     /translation="MTEDCIFCKIIKGEVPANFVYKDEDMAAFHNIKPSAPVHVLIIP
                     VLHIESVNALEERHSAVISKMMIKAKEIAQLLGIRQSGYKLIINVEKGAGQVIFHLHI
                     HLIGGW"
     gene            complement(1844..2143)
                     /locus_tag="VKL21_05305"
     CDS             complement(1844..2143)
                     /locus_tag="VKL21_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45219.1"
                     /translation="MKIITIDPCFTKGDEDLFSGEAELGAIKITQPAIEELKKQFDSV
                     QVTPDNEMIILTNPPVEKIIVFDTGRAIIRRIASKDLAISRFEMLENLLCFGKVL"
     gene            complement(2143..3321)
                     /gene="coaBC"
                     /locus_tag="VKL21_05310"
     CDS             complement(2143..3321)
                     /gene="coaBC"
                     /locus_tag="VKL21_05310"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMB45220.1"
                     /translation="MTSKSLEEKTIVLGITGSIASVRCVELARELRRRGADVHAVMTD
                     AARRIIHPEAMRYATGNAVITEITGNVEHVDFCGFGGRASLLLIAPCTANTIGKIANG
                     IDDTSVTTFTATAFGSGIPIVIVPAMHESMYDHPIVIENIGKLTGLGVEFVNPVTEEG
                     AAKIAGNEEIVLRVERALGKNTLAGKKILITGGATAEAIDPIRILTNRASGRTGVELA
                     LAAFRRGADVTLVHRGCIGIQGICEFYVESAADMTGTVIEELGKGYDLLISAAAISDF
                     TVSPSKDKIKSAKELTLRLKPAPKLIKEARRKYPGLNIIGFKAETGITEDELIKRARK
                     SMDSSHLCMVVANDVSSGGMGTEENDVYLIDGSVKPVSGTKRQIAIEIMDKVEGILGN
                     "
BASE COUNT          925 a          654 c          630 g         1173 t
ORIGIN      
        1 gggtactcat ggtagaagat gtggctacga ccggaggttc tgttttgaaa gctatagaat
       61 tattaagaga tgagggactg gtcgttgagc atgttatcgt tgtggtagat cgtgaagaag
      121 gtgcactttc tgctctttcg aaagctggtg tggaactcat accgcttgcg aaggtaagtg
      181 aactgatccc aacgaaatga atgtaatttc ttaagtagaa ttcaaatcac aatcaagctt
      241 cttccgattt gttgggtaaa cattataagg gttttaagat attatgtgca atatttaatg
      301 agatcggaat ctgagactta tattaagaat caaaacaata aggcatggat taatgaattg
      361 gatttcttga gagcttttgc ggttctggcc gtcataatga tacatacggc tgcaaagttc
      421 aaagataata actctctaaa ttcattagta ttcgtgaatg tttttattga tgttgtttca
      481 cattttgcag ttcctgtgtt tatattgatc tcaggaatgg ttctctcata taattattat
      541 ggacatataa attataaatc atactatatt aaaagattct tttcaataat tcctcaatat
      601 cttttttttt ccttgtttta tctgggtttt tctttcgtta tgggtcatag gaaccctttg
      661 ttttccaaac caatagattt aattctttcc ggagaagcaa gttatcattt atggtttttt
      721 atattgttga ttcaatttta tattatatat ccgtttttaa taaagttgta tttcatatgt
      781 ttatctaaac ataaattaag tttatttata ataatgccat ttattataca gatattattt
      841 tattttctat ctgtttttct tcctgcccca tataataaag atttatcaag tctaattgat
      901 tattatattc ataatgtgtt catttctcat attttttatt ttgtattagg cattcatttc
      961 ggaagaaacc ttcaatcaat gaagaattat atatataatg taaatgataa aatgttatat
     1021 tttttgttgg ttttatcaaa tatatctata tatattataa agataaaagg aatggaattt
     1081 gaaggatctt tagagatcaa tacattgttt ttgctgccta taaagatcat tgagccgctg
     1141 caatatattt taagttttat tttattatgg aaaattgcct caaagttaat agagagaaat
     1201 aatttcgtta ttagattatt gaaagatatc ggaagatttt cttttggtat ttatttggtg
     1261 cacgtttttt ttatcatagt gttaacaaaa attctagagc catataatat tacgttcact
     1321 gactggatat attatccgat aatatttttc tgtgtttcga tattaagtta tttaacgtgc
     1381 aaaataattt cttatacgcc tttcagttat tacattattg ggatcaaatc cccaaaaaag
     1441 aaaaaaaagc aaatagtagc ttaaccgatg gttgttttca ccagccacca atgagatgta
     1501 tatggagatg gaagattacc tgccctgcgc ctttttccac atttattatc aatttataac
     1561 cgctttgccg tatccccagt aattgtgcaa tttcctttgc tttgatcatc attttcgaaa
     1621 taacagcgct gtgtctctct tcaagggcat tgacgctttc gatatgcaaa accgggatta
     1681 taaggacatg aacgggtgct gagggcttta tattgtggaa tgctgccatg tcctcatctt
     1741 tatacacgaa attcgcagga acttctcctt tgatgatttt acagaatatg cagtcctcgg
     1801 tcatgatgtg ttggttgcta tggaatctga agatatcatt aatttacaat acttttccga
     1861 agcaaagtag attctcaagc atctcaaacc tgcttatggc aagatccttg cttgcgatcc
     1921 tccttattat cgcgcgcccg gtatcgaaaa ctattatctt ttcaaccggg ggattagtta
     1981 gtattatcat ttcgttatcc ggtgtcacct gcacgctgtc aaattgtttt ttaagttctt
     2041 cgatcgccgg ttgagttatt tttatagcgc caagctcagc ctcgccgctg aaaaggtctt
     2101 catcgccttt tgtaaagcat gggtcgatag tgatgatttt cattaattcc ccagaatccc
     2161 ttccacctta tccatgatct ctatcgcaat ctggcgcttt gtgcctgaaa caggtttaac
     2221 ggaaccatcg atcaaataaa catcattttc ttctgttccc atcccgcccg atgagacatc
     2281 gtttgcgacg accatgcata gatgagatga atccatggac ttcctggcgc gttttataag
     2341 ctcatcttcg gttatacctg tctctgcctt aaatccgatg atattcaggc caggatattt
     2401 ccggcgcgct tcttttataa gtttaggcgc cggtttcaat ctcagtgtca gctcttttgc
     2461 ggattttatt ttatcctttg aaggagaaac cgtgaaatca gagatagcag cagcgcttat
     2521 gagcagatca tatcctttgc caagttcttc gataactgtt ccggtcatat ctgcggcgct
     2581 ttctacatag aactcgcata tcccctgaat tccaatacag cccctgtgaa caagagttac
     2641 atccgcgcct ctcctgaatg cggcaagcgc cagctctacc cccgttcgtc cggaggcgcg
     2701 gttcgtcaat atccggatcg ggtcaatggc ctcagcggtc gcgccgcctg ttatgagaat
     2761 cttttttcct gcaagggtat tttttcccag ggccctctcc actctcagca ctatttcttc
     2821 atttcccgca atttttgcgg cgccctcttc ggttacagga tttacgaact caactccaag
     2881 tcctgtcagt tttccgatat tttcaataac aatgggatga tcatacatgg attcatgcat
     2941 tgcaggaacg atcacaatcg gaataccgga accaaaagcg gtcgcagtga aggtcgtgac
     3001 ggatgtgtca tcgataccat tagcgatctt tcctattgta tttgctgtgc acggggcgat
     3061 caaaagcaaa gatgccctgc ctccaaatcc gcaaaaatcc acatgttcca cgtttcctgt
     3121 tatttcggtt atcacggcat taccggtagc atagcgcata gcctcaggat gaatgatcct
     3181 gcgtgcggca tcggtcatga cggcatgaac atcagcacca cgccttctta attcccttgc
     3241 aagctcaacg cagcgcacag aagctatgct gcctgtgatc ccaagaacga tggtcttttc
     3301 ttcaagggat tttgaagtca ttattatatg ggttcccatc acattcttat agagtgtaaa
     3361 aagataagaa ggttttgatt aa
//
LOCUS       DATGBN010000221         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_134006, whole genome shotgun sequence.
ACCESSION   DATGBN010000221 DATGBN010000000
VERSION     DATGBN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134006"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..479
                     /locus_tag="VKL21_05315"
     CDS             204..479
                     /locus_tag="VKL21_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45221.1"
                     /translation="MTIPIQIFKVYGILFTLMDAVANLDDLKLELKRELRQEILTEVL
                     DIIREEFYPPEEKIRKEFIKKIDEAERRVKEGKFSKYALGEFEKRFL"
     gene            482..748
                     /locus_tag="VKL21_05320"
     CDS             482..748
                     /locus_tag="VKL21_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015313856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMB45222.1"
                     /translation="MPYIIQLDNTLETTLDKLKKKDISLYLRVIHKIIEISKNPELGK
                     PLRKVLKGKRRVHIGTFVLVYSIDKKNEIVTFLEFEHHDKAYRS"
     gene            complement(999..1517)
                     /locus_tag="VKL21_05325"
     CDS             complement(999..1517)
                     /locus_tag="VKL21_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45223.1"
                     /translation="MVSKTEKSYGCVILQSISIIITLLYLYVINLYPIYSKNEKDIIS
                     YFLLFFVGFFILATLLKSSIGYSMYNRGLFTRFSAVFMILFGLFFLSTLLGFNAAEDL
                     IQEKGKLFEIQLSMKDKMENQLQNKTFIFVMQYNNNYYVMEKNQTTFKTPIIYIIPIE
                     QIEYAKVYRVEK"
     gene            2319..4508
                     /locus_tag="VKL21_05330"
     CDS             2319..4508
                     /locus_tag="VKL21_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HMB45224.1"
                     /translation="MGRRKKMVEHVTELMDKPEFIRNIGTVAHIDHGKTTFSDNLLAG
                     AGMISSELAGEQLFMDFDEEEQRRGITIDSATVSMVHHFEGQEYLINLIDTPGHVDFG
                     GDVTRAMRAVDGAVVLVDAVEGPMPQTETVLRQALRENVKPILFINKVDRLINELKLT
                     PADMQMRLGLVIDKVNKIVKGLKPDDYDRLKLDPVIGKVAFGSALNNWAISIPYMKKT
                     GIGFKDIIELCQAEKQNVLAEKCPLHVVMNDMIITFLPSPVEAQRERIKVIWHGDKES
                     EIGKSMINVDRNGKIALMITDITTDPHAGEVASGRLFSGTLERGKEIFISGMPNANRI
                     QSVGLFMGPERIECDKIPAGNIVAVTGLSDAIVGSTASSDKNMTPFESIRHVSEPVMT
                     VAVEAKHMKDLPKLVEVLRQVAKEDPTLKVMINQETGEHLLAGMGELHLEVVAGRIRR
                     DKHVEITTSKPLVVYRETIAGHAGPVEGKSPNRHNRFYVEVEPLSPTIIELIKNNEVS
                     MRQGEVERRDILMKAGMSKDEAKSIAHIHETNMYIDMTKGIQYLNETMELILEGFEEV
                     MKAGPLSREPVMGVKVKLMDAKLHEDAVHRGPAQVIPASRQAIQAAMLIAGATLLEPF
                     QKVFIHVPHDQMGGAMREMQGRRGSILDMQSEGDMTIIESKAPVAQLFGFSGDIRSAT
                     EGRAMWSTEFVGFEPIPGNMLNEIVMAIRQRKGLKLELPKPSDFISP"
BASE COUNT         1602 a          831 c          950 g         1302 t
ORIGIN      
        1 caaaagcagc acgctaaatc gcatagctac ggttgtgtga ttactttttt aattttttct
       61 gttattagtg tagagtaaaa cggagatttt ctggccccta atcaaactgc acggaaggat
      121 ttcccgtatg caactttcgt ctggggcacc ctggcgcagt tgcatgaggg ttttagtcgt
      181 tacattttgc tatatgaccc cctttgacaa taccaataca aatctttaaa gtctatggaa
      241 ttctatttac tcttatggat gctgttgcaa atttggatga tttaaaatta gaattgaaaa
      301 gggagctgcg acaggagatt ctgacagagg ttctggatat aattcgagag gagttttatc
      361 ctcctgaaga aaaaattcgc aaagaattta taaaaaaaat tgacgaagct gagcgaagag
      421 tgaaagaagg taaattttca aagtacgcac taggagagtt tgagaaaagg tttctttgat
      481 catgccttat ataatccaac ttgataatac tctggagaca acgctggaca aactcaaaaa
      541 gaaggatata tctctttatc tgagagtaat acacaaaatt atcgagatta gcaaaaatcc
      601 cgaattgggc aaaccactcc gaaaagtgtt aaaaggtaaa cggagagttc atattggtac
      661 atttgttctt gtttattcta ttgataagaa aaacgaaatt gtgacttttc tcgagttcga
      721 acatcacgat aaagcatatc gatcctgact caccatcaac ttaatcatcg gataacgcta
      781 tcttaataac ttcaacatca tttttgaacg cttccttaaa atcctcataa caaaagagat
      841 atcgagaaga agactataac ccgataactg tttatccaga ataattaaca gaaaccatat
      901 attattttat aatctcattt tctattgact aaacattgat ttacgttttt ggcttaatat
      961 ttaaataata tttaaaagta atataaaaag gtgggacttc atttttccac ccgatatact
     1021 tttgcatatt caatttgttc aatcgggata atataaataa tcggagtttt aaaagtggtt
     1081 tggttttttt ccattacata ataattatta ttatattgca ttacaaaaat gaaggtttta
     1141 ttctgtaatt gattttccat tttatccttc attgataatt gaatttcaaa taattttcct
     1201 ttttcttgga tcaaatcttc tgcagcatta aatcctaaaa gtgtagataa aaaaaacaac
     1261 ccgaataata tcataaatac agcagaaaat ctagtaaata aacctctatt gtacattgaa
     1321 taccctatag atgacttaag aagagttgct aaaataaaaa atccaacaaa aaataaaagg
     1381 aaataagata tgatatcttt ttcattttta gaatatatag gatataaatt aataacatat
     1441 aaatataata aagtaattat aatactaatt gattgcaata taacgcatcc atacgatttc
     1501 tcggtttttg agaccaaaat ttaaaaaaaa ttgctatgaa aggagataaa attaaaatag
     1561 ggaaaaaacc cattacgaaa taattcgttt gttcaatatt tataaaccga gtggggattg
     1621 ataatttagt aaagtatgaa gaataatatc caatccctgt aaaatagagt aatgcggtta
     1681 taaatataat aaataaaggt aattcgatta tataattaat tttgttagga aaattcttca
     1741 gtgagtcttg attttctgta tctgtcatat tttcatattc tatattattt taattatctt
     1801 ttcttaaata aagctatgta ttcatttaga tttactctat ttaataatct atacaagatt
     1861 ttttagaata aattaatcgc attaattccc atgataagga ataacatctc atagaacatc
     1921 ccgaagggct attcctgcgg gtaggcgcac attcgctttt gcgtgctccc tttagggcgg
     1981 ggggctatct tcatgagttc taagagtgtc catgacgata cgtggccttg cggagcgttc
     2041 atactaaaag taaatgatga gatctttcat aaccgatcat ctggaaagcc ttgttatccg
     2101 aaaaattata atccaatact gccctaactg atttacaggc taataagggt ttaataagat
     2161 tccatagttt attttggata cattttcaaa accacaactt atttcttaat taaaaactac
     2221 ttcacggaaa atatactaaa aattgactaa cattccttag tcttatatag gatagaataa
     2281 tagagcaata aaaattttaa atataagagg taaatcaaat gggcagacga aaaaaaatgg
     2341 ttgaacatgt cactgaactg atggataaac ctgagtttat ccgcaatatc ggtacagttg
     2401 cacacattga ccatggcaaa acaacatttt cagataatct acttgccgga gcagggatga
     2461 tttcatcaga actggctggc gagcaattat ttatggactt cgatgaagaa gaacaaagac
     2521 gcggaattac cattgactct gcaacggttt caatggtcca tcattttgaa gggcaggaat
     2581 atcttatcaa ccttatcgat acaccgggac acgttgactt cggcggcgat gtaacgcgag
     2641 ccatgagggc tgtggatgga gcggttgtac tggttgatgc tgtcgaagga ccgatgcccc
     2701 agacagaaac agtgctcagg caagcgcttc gtgaaaatgt aaagcctatt cttttcataa
     2761 acaaggttga ccgacttatt aatgagttaa aacttactcc agccgacatg cagatgcgcc
     2821 ttggacttgt cattgataaa gtcaataaga tcgtcaaagg cctgaaacct gacgattatg
     2881 atagactgaa gcttgaccct gtaataggta aagttgcttt cggatcagcc ttaaataact
     2941 gggctataag cattccctat atgaaaaaga caggtatagg atttaaggat attatcgaat
     3001 tatgccaggc tgaaaagcag aacgtacttg ctgaaaaatg cccgcttcat gtggtaatga
     3061 acgatatgat cataacattc cttccaagcc ctgtcgaggc gcaaagagag agaataaaag
     3121 tcatatggca tggtgataag gagagcgaga tcgggaaatc catgattaat gtggacagga
     3181 acggcaagat agccttgatg ataacagaca ttaccacgga cccgcatgcc ggtgaagtgg
     3241 caagcggaag gcttttctcc ggtacccttg aaaggggtaa ggaaatattc atttcgggaa
     3301 tgcctaatgc gaacaggata cagtcagtag gcttattcat gggtcctgag cgaattgagt
     3361 gcgataagat ccccgccgga aatatagtag ctgtaaccgg tttgagtgat gcgatcgtgg
     3421 gttcgaccgc ctcatcagat aaaaacatga cgccatttga gagcataagg catgtcagcg
     3481 agcctgtaat gactgttgcc gttgaagcta aacacatgaa agacctgcca aaactggttg
     3541 aagtattgag gcaggtcgca aaagaagacc ccacactgaa ggtaatgata aaccaggaaa
     3601 ccggagaaca tctgcttgca ggaatgggag aactccatct ggaggtagta gcagggagga
     3661 tccggcgaga caaacatgtg gagatcacta catccaagcc gctggtagta tacagggaaa
     3721 cgatcgccgg tcatgccggt cctgttgaag ggaaatcccc caaccgccac aacaggttct
     3781 atgttgaagt tgaaccgtta tccccaacta taatcgaact tataaagaac aatgaagttt
     3841 caatgaggca gggagaagtt gagcgcaggg acatcctgat gaaagcggga atgagcaaag
     3901 acgaagctaa aagcattgcc catattcatg aaacaaatat gtatatagat atgaccaaag
     3961 gtatccagta cctgaatgaa acaatggaac tcatcctgga aggatttgag gaagtcatga
     4021 aggctggacc tttatcccgt gaacctgtga tgggagttaa agtgaaattg atggatgcaa
     4081 aactccatga agacgctgtc cacaggggac cggcgcaggt cattcctgct tcaaggcagg
     4141 caatccaggc agcaatgctc attgccgggg ctactcttct tgaacctttc cagaaagtgt
     4201 tcatccatgt cccgcatgac cagatggggg gcgcaatgcg tgagatgcag ggacggcgtg
     4261 gctctattct tgacatgcaa tcagaaggcg acatgaccat tatcgagtcc aaagcacccg
     4321 ttgcacaatt attcgggttc tccggggata tccgctccgc cactgaaggc agagccatgt
     4381 ggagtacgga attcgtagga tttgaaccaa ttccggggaa catgcttaat gagatcgtaa
     4441 tggctatcag acagaggaaa ggacttaaac tcgaattgcc aaaaccgagt gatttcataa
     4501 gcccatgagg acattacaga ccccgaattt aattacggga cataaaggac tcttaacgga
     4561 aggtttaaaa acaaaaacaa caatacggac acaaaacaat cttcaataaa tatacaggag
     4621 aaatattatg gcagaaaaac cacatttaaa tttagcagtt atcgggcata tcgaccacgg
     4681 gaaat
//
LOCUS       DATGBN010000222         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_327902, whole genome shotgun sequence.
ACCESSION   DATGBN010000222 DATGBN010000000
VERSION     DATGBN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327902"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1845)
                     /locus_tag="VKL21_05335"
     CDS             complement(193..1845)
                     /locus_tag="VKL21_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45225.1"
                     /translation="MNPSDISKILHSLNINPDEISDKKESQTIRILLHIIEELNEEVQ
                     ALKAELQKTRDELNQLKGEKGKPKFSVPKQNKDVSSENERRTPIPRIERKSKEKLSKI
                     KINVTEECRVDPSLLPGDAEFKYYDTVVVQDIIITTKNTAYRKEVWYSPSQHRTYRGE
                     LPHGIEGEFGNGVKSLIITLKHASNVSEPKIHEFLENIGIFISPATISRILTKNLETF
                     HQEKADIFKAGLESTRYQQLDDTGAKVNGQNQFVEIFCNPFYTAYFTIPSKDRLSILD
                     ILQGNKPRSYFFNEEAFTLLESFGLSKIIISKMRDNALDKLLDEKQLQNLINGIFYDP
                     NKGRNIRTRIMEAGAIAAYHNRKDIPVVKVILSDDAPQFKKLAEEQALCWIHDARNYK
                     KLEPIVPLHKKELEDFLNCYWNYYRRLLQFRENPTQENAEQLSTEFDGLVSTKTNYEA
                     LKKRIEITKENRSELLLTLKYPEIPLHNNAAELGARSQVRKRDVSLHTMTQEGTKASD
                     TFMTIVQTAKKLGVSVFDYIGDRVSKSFKLPSLAELIGIKSV"
BASE COUNT          598 a          391 c          363 g          781 t
ORIGIN      
        1 tgcagattcc cacatacaat atctgtacat tttcgtatga ttcttattac ttttcctgaa
       61 aattcagtaa gtttggtctg gaaaattgat aaaatacgag ttttgatatt tcttgctttt
      121 tctaatggtt ttcgtatcct ctccaaaagg agtggtggga ttctgactaa tcaaccaact
      181 ttaattcaaa tttcaaacac tttttatccc tatcaattct gcaagggatg gcaacttaaa
      241 actcttactg actctatctc cgatatagtc aaaaacactt acccctaact tttttgctgt
      301 ctgaacaatg gtcataaaag tatcactggc ttttgtacct tcttgcgtca ttgtatgtag
      361 actcacgtct cgttttctaa cctgagatct tgctccaagt tcggctgcat tattatgtaa
      421 aggaatttca ggatatttca atgtaagaag aagttctgac ctattttctt tggttatttc
      481 aatccgcttt ttcaatgcct cataattggt ttttgtggaa actaacccat caaactcggt
      541 ggataattgt tcagcatttt cttgagtcgg attttctctg aattgcagaa gccgccgata
      601 ataattccaa taacaattta aaaaatcttc aagttctttt ttgtgcaaag gaactattgg
      661 ttctaacttt ttgtagtttc ttgcatcatg tatccaacaa agagcttgtt cctctgcaag
      721 cttcttgaat tggggagcat catcgcttaa tattaccttc accacaggga tgtccttcct
      781 attatggtag gctgctattg ctccagcttc cataattctt gttcgtatat ttctaccttt
      841 attaggatca taaaatatcc catttataag attctgcaat tgcttttcat ctaataattt
      901 atcaagagca ttatctcgca ttttggaaat tattattttc gacaatccaa aagattccag
      961 caatgtgaag gcttcctcat tgaaaaaata gcttcttggt ttgttacctt gcaggatatc
     1021 caaaatactc agacgatctt tacttggaat tgtgaaataa gcagtataaa aaggattgca
     1081 gaatatttcg acaaattggt tctgcccatt tactttggca cctgtgtcat ctaattgttg
     1141 atatcttgta gattccaatc cagccttgaa aatatcagct ttctcttgat ggaaagtttc
     1201 gagatttttc gtgagaatac gagaaatagt ggctggagat atgaatattc caatattctc
     1261 aagaaactca tgtatctttg gctcggatac atttgaagca tgcttgagtg tgatgatcaa
     1321 ggacttcact ccattgccaa attctccctc tattccatgt ggtaactctc cgcggtatgt
     1381 ccgatgttgt gagggagaat accaaacttc tttacgatat gctgtgtttt tcgtggttat
     1441 gataatatcc tgaactacca cagtatcgta atatttgaac tctgcatctc ctggtaagag
     1501 acttgggtcc actctgcact cttcagtaac gttgattttt atcttactca gtttttcctt
     1561 tgatttcctt tcaatgcgtg gaataggagt tcttctttca ttttcggagg agacgtcttt
     1621 gttctgtttc ggaacagaaa atttcggttt acctttttct ccttttaact ggttcagttc
     1681 atctcgtgtt ttctgcaatt ctgcttttaa tgcttgaact tcctcattta attcctcgat
     1741 tatgtgaagg aggatacgga tggtttgtga ttcctttttg tccgaaattt catcaggatt
     1801 gatgtttaat gagtgtaata tttttgatat atcagatgga ttcatgaagg aacactgtaa
     1861 taattcctta gtatttgtat atttctattg catgacggct attgcttggt aataatcaaa
     1921 gaaaaaatga tctaatacca aagattccat aaattatatt tttattttta ccatttaatt
     1981 tgaagaggat accagaattt tatgaagaat gggtgaaaac ataccagaac agctttggta
     2041 aattttattc aattccggcg ccagaaacca ataaagagat attggagaac ctcctgagca
     2101 acgcagagga atcaaataaa atctacaggt cat
//
LOCUS       DATGBN010000223         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_366952, whole genome shotgun sequence.
ACCESSION   DATGBN010000223 DATGBN010000000
VERSION     DATGBN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366952"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            680..1183
                     /locus_tag="VKL21_05340"
     CDS             680..1183
                     /locus_tag="VKL21_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45226.1"
                     /translation="MWDDIRTQQLANNIKQDLDLLKGYEDELRFATEPRLIAKYNHEI
                     ERQRDSLALYQREYDEIIKNAQDVIGSHPVPEKELQDTLNAVQLILSEIRQGKAGQYS
                     PQLVSEAENVSGIVNDPKLDSNHKLKYSIPIIPLILSYEGEVELKGGLDLKNAWHGLK
                     SWLQGKK"
     gene            1180..3216
                     /gene="fxsT"
                     /locus_tag="VKL21_05345"
     CDS             1180..3216
                     /gene="fxsT"
                     /locus_tag="VKL21_05345"
                     /inference="COORDINATES: protein motif:HMM:NF040586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /protein_id="HMB45227.1"
                     /translation="MTDETGKRLKDVEDNIKLDLDVLKDYEDALRYEMDPRRIMKYNR
                     EIQRQRESLARYSKEYEELKRQAAPAQIHKVADLLQQQETKLGMIQELVNKKLLPKIW
                     NVQHLRNNNFTGREDIISELRGALTSGENAAWKQTLTGLGGVGKSQLAVEYIYRHLAD
                     YSIVWWIRSEEATTMATDYASLAQDLELKEKDLKDQPEIVRAVKRWLEHSDGWLLVFD
                     NAKGPEEIRNYLPQGGAGHVIITSRNPNWGNVSKVLPIKVFKRSESIDFLCKRTGNDD
                     TKTAHDLADELGDLPLALEQAGAYIETTVITLKEYLELFLERRKELWKDEFPPLDYPE
                     PVGATWSLAMDRVCKESPEGADILNLCSFLAPDDIPIELILQGTKHIPEPFANAAVDR
                     LAMNRGIRILRQYSLIEAGEDKLSVHRLVQAVVRDRLGQDETKKWAESALRILRNAFP
                     YKSDDIQTWPLSRNLLPHAMAAADHAEELTVAPEETGDILNLTGLYLNEHAESSEAKA
                     NFERALTFYEKVFGHKHSSVANCLNNIGLAWNNLGDSKKAFSYLEQALVINKEVYGER
                     HPDVATSLNNIGAAWDKLGDSNKAISYLEQALVIDKEIYGERHPDVARDLINIGAAWN
                     NLGDSNKAISYLEQALAIDIEIYGERHPNVARDLNRSSQASFLYAWVLLHKLGQ"
BASE COUNT         1081 a          675 c          830 g          900 t
ORIGIN      
        1 ttcatagaat atcgtcttta acttggcctt gccgtagtaa accggaaaaa aacaacgatc
       61 ttaggtcgct caacaaaggt gagacgatag atgcacttag cagcttcctg tttttgccag
      121 taaggagaaa tttgacaaag cctcaatttg ggtattttta agcgacccta aaattatttc
      181 aagatactcg ctcaatcgtt atacaatttt taacttcaaa tagcagagcc ataatttcaa
      241 taactatttg taacctgctc tgtgtcacag atgtaaaagc gttgtttttt tcccatttac
      301 tacgggaagg cctaatagaa agaagtgaaa aaatatggat tggagtaatt ttacgttaga
      361 ttcacagaga tcttccatgg actgcatata gctacgacat tcttatttac tgcatataag
      421 ggttattaaa cgcaggatag tttatttatt gctttacaat gaaggctttc cgtggggaga
      481 aagaccatca aatgcgttgt caccaaatat aatttcggat atatttgaca tatcaatgca
      541 atgaggaagc gttcggagtt tgtggagaag tggtgaatga tattggttaa atagataaca
      601 ttcagcaacg taaccactct aaaaacacat gaacatctaa ttgttcagag tgtaagagtg
      661 gtataactgg tgattagaaa tgtgggatga tataagaaca caacagcttg caaataatat
      721 caagcaggat ctcgatctac tcaagggtta cgaagatgaa ctgcgatttg caacagaacc
      781 gcgcttgatt gcgaagtaca accatgagat tgaacggcaa cgagattccc tcgcacttta
      841 tcagcgtgaa tacgatgaga taataaaaaa tgcacaggac gtaattgggt cccatcctgt
      901 tccagaaaag gagttgcagg acactttgaa tgctgtacag ctgatccttt ctgaaattag
      961 acagggtaaa gcagggcagt acagcccgca gttggtgagt gaagccgaga atgtttctgg
     1021 gattgtcaat gatcccaaac tcgactcaaa tcataaactc aaatattcaa ttcccatcat
     1081 cccgcttatt ctatcctacg aaggagaggt agaactgaaa ggaggattag acttgaaaaa
     1141 tgcctggcat ggcttaaaat catggctgca gggtaagaaa tgacagatga aactggcaag
     1201 cgtctgaaag atgttgaaga caatatcaaa ctggatcttg atgtgctcaa ggattatgag
     1261 gatgcactga gatacgagat ggacccacgc cggataatga agtacaatag ggagattcaa
     1321 cgacagcgtg aatctcttgc acgatactcg aaggaatatg aggagctgaa aagacaggct
     1381 gcacctgcac agatacacaa ggtagctgac ctcttgcagc aacaggaaac aaaactgggt
     1441 atgattcagg agctggtgaa taagaagctg ctcccgaaga tatggaacgt tcaacattta
     1501 cgcaacaaca atttcacagg ccgggaggat attatttcag agctgcgggg agcactcact
     1561 tcaggagaga atgctgcatg gaagcagacc ctaacaggcc ttggaggtgt gggaaaatcg
     1621 cagcttgcag ttgagtacat ttaccgccat ctggcagatt acagcatagt ctggtggatc
     1681 cgatctgagg aggcgacgac tatggctact gattatgcca gtctcgccca ggatctggaa
     1741 ttgaaggaaa aagatttgaa agatcaacct gaaatcgtca gagctgtaaa acgctggctg
     1801 gaacacagtg atggctggct tctcgttttt gacaatgcaa aaggtcctga agagatccga
     1861 aattatcttc cccagggagg cgccggtcat gtgatcatca cttctcgcaa tcccaactgg
     1921 ggtaatgtat caaaagtgct gcctattaag gtatttaaaa gatctgaatc catcgacttt
     1981 ctctgcaagc gtactggaaa tgatgatact aagaccgctc atgatcttgc agatgagctt
     2041 ggggatctgc cccttgctct tgagcaggca ggtgcttata tagaaacgac agttattacg
     2101 ttgaaagaat atctagagct gttcctagaa cggcgaaaag agctatggaa ggatgaattt
     2161 cctcctcttg actatcctga acccgtgggg gccacctggt ccctagcaat ggatcgtgtc
     2221 tgtaaagagt caccggaagg tgcagatatt ttgaacctct gctcttttct ggcccctgat
     2281 gacatcccta tagaacttat acttcaagga acgaagcata tccctgaacc gtttgcaaat
     2341 gcagcagttg acagacttgc catgaacagg ggcataagga tcctgcggca gtattcccta
     2401 atagaagcag gcgaggataa attgtcagtt cataggctgg tccaggcagt ggtacgtgac
     2461 cggctgggtc aagatgaaac taaaaaatgg gctgaatcag ctctgcggat tttgagaaat
     2521 gcttttccct ataagagcga cgatattcag acctggcctc tatccagaaa tcttttgcct
     2581 catgctatgg ctgcggctga tcacgctgaa gaactcacgg ttgccccgga agaaacggga
     2641 gacattttaa atctaactgg attgtatttg aatgagcacg ctgaatcttc tgaggctaag
     2701 gctaactttg aaagagcact gactttttat gaaaaagtat tcggtcataa acattccagc
     2761 gtggctaatt gtttgaacaa tatcggcctg gcatggaata accttggaga ttcgaaaaaa
     2821 gccttttcat atttagagca ggcgctggta attaataagg aagtctatgg agagaggcat
     2881 ccggacgttg caacctccct caataatatc ggcgcggcat gggataaact tggagattcg
     2941 aataaagcca tttcatattt agagcaggcg ctggtaattg ataaggaaat ctatggagag
     3001 aggcatccgg acgttgcaag agacctcatt aatatcggcg cggcatggaa taaccttgga
     3061 gattcgaata aagccatttc atatttagag caggcgctgg caattgatat agaaatctat
     3121 ggagagaggc atccgaacgt tgcaagagac ctcaataggt cgtcccaggc aagttttctg
     3181 tatgcttggg tgttgttgca caaactggga caatgagcca tgtgatgaaa gcgtttttat
     3241 cgaccccaca acctctttct ttggaataaa gtttcccatt tttaccccca tctgcaagtc
     3301 caagattttg ggaagggaaa aggagaaaga attacgtccc gatgactcaa acgaaaataa
     3361 tgatagtatc ctggtgtgag gctgggttca ggggaattag agaggtgaca tagaattata
     3421 gcatattcaa agatagcaat gccttaattg aactgagatc gtttcttata tcgcatccta
     3481 atgaat
//
LOCUS       DATGBN010000224         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_483031, whole genome shotgun sequence.
ACCESSION   DATGBN010000224 DATGBN010000000
VERSION     DATGBN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483031"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VKL21_05350"
     CDS             complement(<1..223)
                     /locus_tag="VKL21_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMB45228.1"
                     /translation="MGTITTKDGTQIYYKDWGTGQPVVFSHGWPLSADSWEAQMLFLA
                     SNGYRCIAHDRRGHGRSSQPWSGNEMDTYA"
     gene            654..1007
                     /locus_tag="VKL21_05355"
     CDS             654..1007
                     /locus_tag="VKL21_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45229.1"
                     /translation="MNIDISPNLEKDFKKFVLEKHGKLRGKLGEEMEKALSFYLTHQE
                     KTDTQDATTNAQLEKQLESDMQVISSAYQTNNDIQNTGVDVEKANGVAAKIVVDVERA
                     QVDAEKVWEQQNTGT"
     gene            1289..1657
                     /locus_tag="VKL21_05360"
     CDS             1289..1657
                     /locus_tag="VKL21_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45230.1"
                     /translation="MQEEQEEIKEIKRTLSDLTSKFEDKFDELSKEVKEVEEQQENDE
                     EKINENAAQEGRIASLESTRAGGHAAAQAGLLLKRMDSLEKHAGATTSPDPRVDALEP
                     QLEGMETRIATLEHKKQSDK"
     gene            2015..3613
                     /gene="atwA"
                     /locus_tag="VKL21_05365"
     CDS             2015..3613
                     /gene="atwA"
                     /locus_tag="VKL21_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HMB45231.1"
                     /translation="MTLFIEIKNLRVGFDGIDVLKDINLDIAEGEIIGILGKSGGGKS
                     IFMLVLRGVDAFDDISGSVIYHLSRCNKCGLIERPSNVGTACPRCNSSLTKLEADFVK
                     LKIYDPLRRDITRKIAIMLQRTFALYGDEKVIVNVMNALQEIGEIGPNAINKAAELID
                     QVNLSNRIMHIARELSGGEKQRVVLARQLVKDPILLLADEPTGTLDLKTAELVNKTIL
                     DATRNYRISLIITSHWPRVIEELSHRAIWLDDGMIKQIGASRDIAASFMKEVGEIGGH
                     ENVEIGQPIIRVCNLKKTYFSLDRGIVRAVNDICFEVNEGEIFGLIGVSGAGKTITSK
                     IVSGLLRPTSGTVEVRIGDDWIDMTQLGPDKKGRATKYIGVLHQEYSLYPHRTVLDNL
                     TESIRLELPPKLGKRKAIQTLMTAGFTEKKANEILTKMPTELSVGEKHRVAMTQVLIK
                     EPRILVLDEPTGTMDPVTKIEVAKSILKARKEFGETFIIVSHDMDFVAQICDRVALMG
                     LGEIVDIGDTDAVLSRISKKEASL"
BASE COUNT         1240 a          715 c          860 g          988 t
ORIGIN      
        1 cggcataggt gtccatctcg ttgccactcc agggctggct cgaccggcca tggccgcgac
       61 ggtcatgggc aatgcagcga tagccgttgg aggccaggaa cagcatctga gcttcccaac
      121 tatccgcact gaggggccag ccgtggctga aaaccacagg ctgtcctgtg ccccagtcct
      181 tgtaatatat ctgcgtacca tctttcgttg ttatcgtacc cattatttgc ctcctttttt
      241 aacagccaac cgagacaacg ctaaactctc aaagcttcca gccctggata gtttacactc
      301 tgtctttttg ccaaggtgaa cctcctctta agtttcataa ttattgagat ttatacctat
      361 cggtcaaaat taaagcgttc attagctttt tcatccacac tcaaccccat caaagggaag
      421 tgatacaatc tttttttcag ggactttgta tgcccacaaa ctatatacat tttcctccag
      481 gattcgatat gagaggcgat cgtgacacgg gaaggtaagc aaggattacc ttatatttta
      541 taaattttgt tcataatcgc tattcagcga ggttataaaa taagcatctt atactcaaat
      601 taactaccta cttgaatagt taaatattta atattagaaa atcaagtatt ctcatgaaca
      661 ttgatatatc gccaaatttg gaaaaggatt ttaaaaaatt tgttctggaa aagcacggaa
      721 aactacgtgg caaattaggc gaagagatgg aaaaggctct ttcattctat cttactcatc
      781 aagaaaagac cgatactcag gatgcaacaa ctaatgcaca actcgaaaaa caacttgaga
      841 gcgatatgca ggtaataagt tcagcatacc agaccaataa tgatattcag aacaccgggg
      901 tcgatgtaga aaaggcaaat ggcgttgcag caaagatagt tgtcgatgta gaaagggcac
      961 aggtcgatgc agaaaaagtg tgggaacaac aaaataccgg aacatgagga gttttcgaga
     1021 cccacttaca tactcattac gatgacgtgc gcctcaaact catgtttctc cttttttctt
     1081 tttccttcca attggtttca tggtttcatt atgatcgcta attatatttt ggagaaatat
     1141 catttaattg aattaaaagt aaatgaacga tcagatattt ataaatacga tttaaaccta
     1201 tagtatatat attgtttgcc acaagtgaaa aaaatgtggg ggaatttacc tatacaaatt
     1261 tttttcgtta cattgtgggg gctaatttat gcaagaagaa caagaagaaa ttaaagagat
     1321 aaagcgtaca ctcagtgact tgacctccaa atttgaagat aaatttgatg aactatctaa
     1381 agaagttaaa gaagtcgaag agcaacaaga aaatgatgaa gaaaagataa atgaaaacgc
     1441 ggcgcaggag ggacgtatcg cctcgctgga gagcacgcgt gccggtgggc atgccgcagc
     1501 tcaagcgggt ttgctgttga aacgtatgga ctcgctggag aagcatgctg gagcgaccac
     1561 ctctccggac ccacgcgtcg atgctctgga gccgcaactc gaaggcatgg agacacgcat
     1621 cgccactctg gagcacaaaa aacagtcaga taaataagaa attgaaggaa tactaagaaa
     1681 agcagcacaa gagatcaggt tgaaagcaaa gttcataaaa taaagggtta aatcaaggaa
     1741 actgccgggg agctcctcga taatcccaaa ctcgaagtag aaggcacagc agaaaaaagt
     1801 atcggcaaag ttcaagaaat tactgaatat ttcaatgaat tattcagttt atacgggatt
     1861 attgatagaa aagatagaac ttattgaaga tttttataat ttggagtttt gggacagccg
     1921 gggaacctgg gaagatagat ttaaaatcat ttgagtacaa ttacttataa gataaattct
     1981 ttaattgggg gatataacac agggagaata aaatatgacg ctctttattg aaattaaaaa
     2041 ccttcgcgtc ggttttgatg ggattgacgt gttaaaggat ataaaccttg atattgcaga
     2101 gggagaaatc atcggcatac tgggaaagag cggagggggt aagtccattt tcatgctcgt
     2161 gcttagagga gtggacgcct ttgatgatat ttcaggaagt gtaatctacc atctctccag
     2221 atgcaataaa tgtggattaa tagagcgccc gagtaacgtt ggtacagctt gcccaagatg
     2281 caatagttca cttacgaaac ttgaggcaga tttcgttaaa ctaaagatat acgatccgct
     2341 caggagagac attacaagaa aaatcgcgat catgctccag aggacattcg ctttgtatgg
     2401 tgatgaaaaa gtaatagtca acgtaatgaa tgcgcttcag gagataggcg aaattgggcc
     2461 taacgcaatt aataaagcgg ctgaactgat tgatcaggtg aacctctcta acagaataat
     2521 gcatatcgca agagagcttt ctggcggtga gaagcagcgc gttgtacttg cgagacagct
     2581 tgtgaaagat ccaatactgt tattagcgga cgaacccaca ggaacgcttg atctcaagac
     2641 agctgaactt gtcaataaaa ccatcttaga tgcaactcgt aattacagga tttcacttat
     2701 tatcacttct cactggccaa gagttatcga ggagctttca catcgtgcaa tctggcttga
     2761 cgatggcatg attaaacaaa tcggtgcttc tcgggatatt gccgcatcgt ttatgaaaga
     2821 agttggcgaa ataggcggtc atgaaaatgt ggaaatcgga caacccatca ttcgtgtatg
     2881 taacctgaaa aagacatatt tctccctcga taggggcatc gtgagagcag tcaatgacat
     2941 ctgtttcgag gtaaatgaag gcgagatatt tggtttaatc ggtgtcagtg gggcaggcaa
     3001 aactataact tcgaagatag tatcaggatt gcttcgccca acatcaggta ctgttgaggt
     3061 caggattggc gacgattgga ttgatatgac gcaactgggg ccagataaga aagggagagc
     3121 taccaaatat attggtgtac tacaccagga atacagtctc tatccacaca gaaccgtgct
     3181 tgataacttg acagaatcca ttaggcttga attgcctcct aagcttggta agagaaaggc
     3241 aatccagaca ttgatgaccg caggtttcac agaaaagaaa gcaaacgaga ttcttaccaa
     3301 gatgcctact gaactgagcg ttggagaaaa gcacagggtt gcgatgaccc aggtacttat
     3361 aaaagagccg aggatattgg ttttggatga accaacggga acaatggatc ctgtaacgaa
     3421 gatagaagtt gcaaaatcca ttctcaaagc cagaaaagag tttggagaga ccttcattat
     3481 agtatcgcat gatatggact ttgttgcaca gatatgcgac agggtagctc tgatggggct
     3541 tggtgagatt gtggatattg gggatacaga tgctgttttg tcaagaatta gtaaaaagga
     3601 agcttcatta taagcttgct aatgcccaag atcagctatg ctttcatact tcatcgatta
     3661 agccctaact ccatatgatt ttacagaaat ggggttcaaa gggggaagaa agctcctgac
     3721 ttcagtcgtg gagatgaatt tcccctttgc aataaatacg gaatactttc gcatcgctca
     3781 gccaagactt aagaccaaaa cta
//
LOCUS       DATGBN010000225         5480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_95252, whole genome shotgun sequence.
ACCESSION   DATGBN010000225 DATGBN010000000
VERSION     DATGBN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95252"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..981
                     /locus_tag="VKL21_05370"
     CDS             316..981
                     /locus_tag="VKL21_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 domain-containing protein"
                     /protein_id="HMB45232.1"
                     /translation="MISIGVITRGKYGLRLIENIRKHSGFKVSSLELPETLPDFIEDP
                     SGFVNGLDKSFFSNDLIITYIMHPDITPEIVRLAGQEGAKAVIIAGGLARAGGKDELS
                     DISKKYGIPIEIHEICCDIGSSGNNVVKEFISIFGRPQIKVTTKDGLISNIEVIRGAP
                     CGSTWHMAKNLIGSKIHDAPAKGGLLMQQYPCRAIRGTRSGIHKAAKLHKEAVEKALI
                     EGC"
     gene            982..2208
                     /locus_tag="VKL21_05375"
     CDS             982..2208
                     /locus_tag="VKL21_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMB45233.1"
                     /translation="MEGSIYERSLKFHEAHQGKIALKSKVDIKTTDDLCLAYTPGVAQ
                     ACMRIHSNRDDIYRYTSKGNFVAVISDGTSVLGLGDLGSYAALPVMEGKAILFKIFAG
                     VDAFPICLDTRDTEEVINIVKNIAPVFGGINLEDIGAPRCFEIETRLSALLDIPVFHD
                     DQHGAALVMLAGLMNSLKIVGKNFNEIKVVISGAGAAATASAKLLVNKGVRDMIMCDS
                     TGIIYEGRAGMNPYKEELARLTNKEQMAGSLADALKGADVFIGLSVADIVSEEMVRSM
                     SDDAIVLPLANPTPEIMPEKAKRAGARIVGSGRSDCPNQINNALGFPGIFRGALDTRA
                     RGINEEMKLAAANTLASLVFEPSEEKIIPSIFDAEVAPAVASAVARAAMESKVARKRV
                     SPEEVARHTKELVKNL"
     gene            2205..2813
                     /locus_tag="VKL21_05380"
     CDS             2205..2813
                     /locus_tag="VKL21_05380"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMB45234.1"
                     /translation="MKFTNFLIISCISLSLYFWGTKENLAFSEYALFHGAYYTLLTGL
                     FVHANAVHLFGNMVFLYVFGNSLEDEVGPFNTAIVFFTGGFLSFIISIPFYPGSNMVG
                     ASAAIFSLMAALLLARQPGYSVQFLSPIGPLALLYLFFNIIAVKNDTGGNVANISHII
                     GFMIGMFFGAQWNKKWRESLFLTLALLVGYIVSYNYLKEWVF"
     gene            2882..4165
                     /locus_tag="VKL21_05385"
     CDS             2882..4165
                     /locus_tag="VKL21_05385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HMB45235.1"
                     /translation="MKISVIGTGYVGTVSAACFAELGHKVVCVDIDSSKIEKINAGIP
                     PIYEEGLTDLMKKHAGKKLSATSDYRFAVMNSDVSFICVGTPSDADGNIDLGIVKAAS
                     SSLGEALKDKKDYHVIVVKSTVVPETTEKVILPIIEKHSKKRSEDFGIAMNPEFLREG
                     KAIYDFMHPDKIVVGSMDKRSGDLVASLYNGLNCEVTRTNPRTAEMIKYVNNAFLATK
                     ISFSNEIGNICKLLDINTYEVMKAVGKDSRIGPHFLNSGAGFGGSCFPKDVKALIGKA
                     KDTGYEPLILESVIKVNEKQPGRMIVLLKKELGNLKGKKIALLGLAFKNDTDDIRESR
                     AIPVIKELLDNGAQVSAYDPMANEFMSRLFGDIKYYSNAADALRDSDGCLIMTEWDEF
                     KSLDKEFDLMRNKLIIDGRHMLAGRKGIKYMGLCW"
     gene            4179..5195
                     /locus_tag="VKL21_05390"
     CDS             4179..5195
                     /locus_tag="VKL21_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMB45236.1"
                     /translation="MKNKIKVVIPAAGAGKRLFPHTFTKPKPMVYIAGKPIIGHILDR
                     MKDVKPEEIIIIVGYKKEQIISYVNANYSKEFNIRYIDQNDQLGLGHSIYMARDVIGD
                     SDIMIALGDMIFKAGYIEFYNHYLKNGDTSASIGVREVEDPTKYGIVELEGNYIKSLE
                     EKPARPKSNMGIVGVYFIKETRILISIIEEMILNNKRNRGEYQLTDALQEMINRGYHL
                     KVFPVSSWYDCGHSESLLETNRVLLDEKEDVDHIHQIKDSVVIQPVAIGDNVRIINSV
                     VGPHASIASDSHIENSIISDSVIGSRSHISKVNLQRSIVGDDASVHGKHNSLNIGDSS
                     SIEF"
     gene            5217..>5480
                     /locus_tag="VKL21_05395"
     CDS             5217..>5480
                     /locus_tag="VKL21_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMB45237.1"
                     /translation="MKVLVTGGAGFIGSHLCDLLLARGHEVLCVDNLATGNKKNIIHI
                     ENDNFSFIRHNVTKPLYLEEKIEWIFHLASPASPIDYLELPIPT"
BASE COUNT         1702 a         1053 c         1296 g         1429 t
ORIGIN      
        1 tatcgataga taaatagata tcatgtcatc ggtcaagaga aaatccatga tatagaacac
       61 ggatctccac gtatgtcaag agctatacgt caagggttgc gccccttgag gacgcgtcca
      121 cgtatgccac tggcatacgt gtgcacggcg tcaactgacg tgacggttgg gcgacgtcaa
      181 cttgcgcaac ggttgacgcg gatgcgcacg gattatccgt gtcatccgcg ttctatttgt
      241 attaaattgg agatttaata gaaatcatat ggcagcagaa aaaaattccc agctaacggc
      301 tattaagaca caaaaatgat ttccatcgga gtaataacgc gaggaaaata cggtttgcgc
      361 ctgatcgaga atatcaggaa gcattccgga tttaaagtct catcattgga gcttccggaa
      421 acacttcctg atttcataga agatccatca ggatttgtaa atggattgga caaaagcttt
      481 ttttcaaatg acctgataat aacttacatc atgcatccgg atattacgcc cgaaattgtc
      541 cgccttgcgg gccaggaagg ggcaaaggca gtaattatcg caggaggcct ggcaagagca
      601 ggcggtaaag atgagctttc ggacatttcc aaaaaatatg gcatccccat cgagatacac
      661 gagatttgct gtgatatcgg atcatcaggg aataatgtgg taaaggaatt tatatccatt
      721 ttcggaagac cacagatcaa agttaccaca aaagacggtc taatatcgaa cattgaagtc
      781 attcggggag ccccatgcgg aagcacatgg catatggcaa aaaatcttat cgggtcaaaa
      841 atccatgatg ctcctgcaaa aggcggcctt cttatgcagc agtacccatg cagggcaatt
      901 cgaggcacca ggagcggaat acataaagct gcgaagctgc ataaggaagc ggtagaaaaa
      961 gcattaattg agggttgtta aatggaagga tcaatttacg aacgctcact caaattccat
     1021 gaagcgcatc agggcaagat cgctttgaaa agcaaggtgg atataaagac aacagatgac
     1081 ctctgtcttg cttacacgcc aggtgtggcg caggcatgca tgaggataca ttcgaacagg
     1141 gatgatattt accggtatac ttcaaaaggc aattttgttg ccgtaataag tgatggcaca
     1201 tcggtgctgg gattgggaga ccttggaagt tatgccgccc tgccagtgat ggaaggaaaa
     1261 gccatacttt ttaaaatttt tgctggcgtg gatgctttcc ccatatgcct tgataccagg
     1321 gatactgaag aagtcatcaa tatagtaaag aacatagccc ctgtttttgg gggaataaat
     1381 cttgaggata taggcgctcc gcgctgtttt gagatcgaga cccgactttc ggcattactt
     1441 gatattcccg ttttccacga tgaccagcat ggcgcagcgc tagtcatgct cgccgggctg
     1501 atgaattcct taaagatcgt gggaaaaaat ttcaacgaga taaaagttgt tataagcggc
     1561 gcgggagcgg cagccacagc gagcgcaaaa ctactggtaa acaaaggagt gagggatatg
     1621 atcatgtgcg attccaccgg cattatttat gaaggcaggg ctggaatgaa tccctataaa
     1681 gaagaactcg ccaggcttac caataaggaa cagatggcgg gttcccttgc cgatgcattg
     1741 aaaggagcgg atgttttcat aggcttatcc gtggctgata ttgtttctga ggaaatggtg
     1801 cgatccatgt ctgatgatgc aatagtcctg ccgctggcca atcccacccc tgagatcatg
     1861 ccagagaagg caaaaagagc cggagccagg atagttggtt cagggcgctc ggattgcccc
     1921 aaccagatca ataatgccct tggattcccc ggcatattca ggggggctct tgatacaaga
     1981 gcaaggggca tcaatgaaga aatgaaactt gcagcggcaa atacgctggc atcgctcgtc
     2041 tttgaacctt cggaggaaaa aataatccca agtatttttg atgcggaagt tgccccggca
     2101 gttgccagtg ctgttgccag ggccgcgatg gaaagcaaag ttgcaagaaa acgcgtatct
     2161 cctgaagaag tggcaagaca tacaaaagaa ttagtcaaga atctatgaaa ttcacaaatt
     2221 tcctgataat atcgtgcata tctttaagcc tatatttttg gggcacaaaa gagaatcttg
     2281 cattcagtga atatgccctt tttcacggcg catattatac tttgctgaca gggctttttg
     2341 ttcatgcaaa tgcagtacat cttttcggga atatggtttt cctttatgtt ttcggcaaca
     2401 gtcttgaaga tgaagttggg cccttcaata ccgcaatagt tttttttacg gggggctttc
     2461 tatctttcat tatcagtata ccattttatc cgggctcgaa catggtgggg gcatcagcgg
     2521 ccatattttc attgatggca gcgcttctgc ttgcccgcca acccgggtat tccgtccaat
     2581 tcctctcacc gataggtccg cttgcacttt tgtatttgtt tttcaatata attgcagtca
     2641 aaaacgatac aggaggaaat gttgcgaaca tttcccatat cattggtttc atgatcggaa
     2701 tgtttttcgg ggcgcaatgg aataagaagt ggagagaaag cctgtttctg acgcttgcat
     2761 tacttgtcgg gtatatagtt tcatataatt acctgaagga atgggttttt taaatatgga
     2821 tgatcaattt gataaatatg caaaagatta atataaaaat aaaatgtaat aggaataatt
     2881 tatgaaaata tcagtaatag gcacaggtta tgttggaacg gtcagtgcag catgttttgc
     2941 tgaacttgga cacaaagttg tttgcgtgga tattgacagc tcaaagatcg agaagataaa
     3001 tgcaggcatt cctccgatat atgaagaagg actgaccgat cttatgaaaa aacatgccgg
     3061 caagaaattg agcgcaactt ctgactacag gttcgcagtt atgaattcag atgtttcatt
     3121 catatgtgtg ggtacgccat ctgatgctga tgggaatatc gatctgggta tcgttaaagc
     3181 cgcaagttcc agccttggtg aagcgttaaa ggataaaaaa gactaccatg tcattgtcgt
     3241 aaaaagcacg gtggtaccag agacaactga aaaagtaata ctgccaatta ttgaaaaaca
     3301 ctcaaaaaaa cgttcagaag atttcggtat agccatgaat cccgaattcc tgcgcgaagg
     3361 aaaggcaatt tatgatttca tgcacccgga caaaattgtt gtgggttcaa tggataaacg
     3421 atccggggat ctggtggcat ccctttacaa tggtcttaat tgtgaagtaa caaggacaaa
     3481 cccaagaact gctgaaatga taaaatatgt aaataatgcg ttcctggcca caaaaatatc
     3541 tttttcaaat gaaataggaa atatctgtaa actattggat atcaatacat atgaagtaat
     3601 gaaagctgta ggaaaggatt ccaggatcgg accgcatttc cttaactccg gcgcgggttt
     3661 tggagggtca tgtttcccga aggacgtaaa agcgcttatc gggaaggcaa aagacacggg
     3721 atatgaacct ttgatccttg aatcggttat caaggtaaac gaaaagcaac ccggccggat
     3781 gatagtattg cttaagaaag aacttgggaa tctgaaaggc aagaagatcg cactactggg
     3841 attggctttt aagaacgata cggatgatat cagggaatca agagctattc cggtaattaa
     3901 agaattgctt gataatgggg cccaggttag tgcttatgat ccgatggcaa atgagttcat
     3961 gagccggctt tttggggata tcaaatacta ttcgaatgcg gcggatgcct tgagagattc
     4021 agacggctgc ctcataatga ctgaatggga tgaattcaaa tcgcttgata aggaatttga
     4081 cctgatgaga aacaaactga tcatcgacgg ccgccatatg cttgcgggac gaaaaggcat
     4141 taaatatatg ggcctttgct ggtaataagg agaaattgat gaaaaataaa atcaaagtag
     4201 ttattcctgc ggccggagca ggcaaaaggc ttttccccca tacgttcaca aaacccaagc
     4261 ccatggtata tatcgcagga aaacccataa ttggtcatat acttgacagg atgaaagatg
     4321 taaaacctga agaaataatt attattgtag gctacaagaa agaacagatt atatcttatg
     4381 tgaatgctaa ttattcgaag gaattcaata tccgttatat tgaccagaat gatcagcttg
     4441 gccttggtca ttccatttat atggcaaggg atgtcatcgg ggattccgat attatgatcg
     4501 cgcttggcga tatgatattt aaggcagggt atattgaatt ttacaatcat tatctgaaaa
     4561 acggggatac atcagcttca attggagtaa gggaagttga agatcccaca aaatacggaa
     4621 tagtcgaact tgaaggaaat tacataaaaa gtcttgaaga aaaacccgcc cggccaaaat
     4681 ccaatatggg tatcgtgggt gtatatttta ttaaagaaac ccggattctt atatcaataa
     4741 ttgaggaaat gatccttaat aacaaacgaa acagaggtga ataccagctc actgacgcac
     4801 tgcaggaaat gataaacaga gggtatcatc tcaaggtatt ccctgtatca agctggtatg
     4861 attgcggaca ttccgagtcc cttcttgaaa caaaccgcgt gcttcttgat gaaaaagaag
     4921 atgtggatca tatccatcaa ataaaggatt cagttgtaat tcaaccggtc gcgatcgggg
     4981 ataatgtcag gatcatcaat tcggtcgtag ggcctcatgc ttcgatcgca agtgattccc
     5041 atattgagaa cagtataatc tcagacagcg taataggttc ccggtcccat atatcaaaag
     5101 tcaatctcca gaggtccata gtgggagatg acgccagcgt acacggcaag cataattccc
     5161 tgaatattgg tgattcatcg tctattgaat tctaggcata ttattacgag gcatatatga
     5221 aagtattagt cacaggtggg gcgggattta tcggatcgca tctatgcgac ctattgcttg
     5281 caagaggcca tgaagtccta tgtgttgata acctggccac gggaaataaa aagaacatta
     5341 ttcatattga aaatgataat ttttcattca tcaggcataa tgtgacaaaa cctctatatc
     5401 ttgaagaaaa gatcgaatgg atttttcatc ttgcaagtcc ggccagtccc atagattacc
     5461 tggagctgcc catccccacc
//
LOCUS       DATGBN010000226        15507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_522336, whole genome shotgun sequence.
ACCESSION   DATGBN010000226 DATGBN010000000
VERSION     DATGBN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15507
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522336"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VKL21_05400"
     CDS             complement(<1..1164)
                     /locus_tag="VKL21_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45238.1"
                     /translation="MNPTEMVFEEWKTYAGKKALKKRALDLSGLISNSRLTIIGITGI
                     RRSGKSSVLIMLQQELVKKGKNTAYINLEDSRIKDNISVLDDVLKWFGDTGFLVLDEI
                     TGVHDWEGWLARNHELLKGRLHLIVSSSRKKLVIPSRPLRGRMLHYEMYPLSFREFMS
                     FKGHKVELTVAGIGRIEQALDEYLIFGGFPEVVLTDDKTDKIQILNSYFRDIIGLDLA
                     EMSDNSITTVELFGKYIIESPYFSASKSLNFFKGVGHKIGKESLLDLEKYSQEGYLFF
                     FVSIFSHTIKDRSQYPRKAYLGDMGFMYSMSGRIDYGRLFENAVYLELKRDMPQQYDI
                     HYWKNTQGFEVDFVIREGLAIKEIIQVTYELNREKTKYREVRALAAFAREFGLD"
     gene            1472..3928
                     /locus_tag="VKL21_05405"
     CDS             1472..3928
                     /locus_tag="VKL21_05405"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006690.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HMB45239.1"
                     /translation="MTIEILESYQNHIAERQKQGIPPLPLNAEQVKALIPLFFDPPSG
                     SEDLLLELLKNHIPPGVDPAALEKAKFLRQIALREKRSKLLLPHEAVSLLGLMKGGYN
                     IETLIELLKDKDLADQAVNALSNTILIFGFFDRIKELSATNPGAKKVLESWAKADWHK
                     GEIPQTKKVTIFKIPGEVNTDDFSPAKRAGTRADIPLHALSMFENRYPGAIDEIMALK
                     NKGYPIAFAADVVGTGSSRKSAANSLIWWIGDDIPGIPNKRKGGIILGSQIAPIFFNT
                     ARDSGAIPIKCDVCALTSGDVVTLDFKQGKVLGENKEEIASFSLEPQTLPDEYGAGGR
                     LSLIIGKELTKKAQEALGVNYDLFIKPGEPATSDRGYTLAQKIVGKACGYPGVRPGQS
                     CLPCITTVGSQDTTGPMTVDEIKELVCLGFQAPLVMQSFCHTAAYPTTADTLMHKELS
                     TFFKERGGVVLKPGDGIIHSWLNRMILPDTVGTGGDSHTRFPIGISFPAGSGLVAFAA
                     SLGVMPLDMPQSVLVRFKGKLKKGLYLRDVVNAVPYYALKQGKLTLEKKNKKNIFNGR
                     IMEMEGLPDITVEQAFELTDASAERSVEAAVIALGKEQVAIFLETNIQILRSLIKEGY
                     ASNTLEMRIKAMQEWLDAPFLMEADPDAEYADILQIDLSDINEPFIACPNDPDFVKPL
                     SEVQGVKIDEVFIGSCMIGIEQLDRAAKILEKAGSIKAKLWVAPPTRLIEKTMKEKGN
                     YSRLTGLGARMEIPGCSLCMGNQARVADNAIVFSTSTRNFDNRMGKNAQVYLGSAELA
                     AITAVLGRIPTLSEYHKLLG"
     gene            complement(4185..4883)
                     /locus_tag="VKL21_05410"
     CDS             complement(4185..4883)
                     /locus_tag="VKL21_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KaiC domain-containing protein"
                     /protein_id="HMB45240.1"
                     /translation="MDSLNTGVTGLDEMLCGGIPKEHIVAVLGSPGTGKSTFALQFIY
                     AGLLKDENCIYLSLEESEETIVKTASIFGWDLKPYITNKKLVLIRLSAMNIKITANRV
                     ENDLPRLFESFGVKRLVIDPITLYEMLYENESERREHLFNFAEKIREHGITVVMTSET
                     HHNNAYNSKFGLIEYIADGVIVLRQVRQTDLRAVTTVIEVTKMRRIDHSRDIKPYVIS
                     KNGIIVHSEAEVFS"
     gene            complement(4883..5701)
                     /locus_tag="VKL21_05415"
     CDS             complement(4883..5701)
                     /locus_tag="VKL21_05415"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB45241.1"
                     /translation="MDLKKNPTGIDSLDPVLQGGFPSGSLVLLLGEIGAGEFEFAITS
                     IARLLNRPKAGNLFPGKVCYVSFTRGKDDVMKEVAFSFPEFYNMIRDRLEFKDFSEAF
                     FARSFIPINWRSSSQTELSFDSLKWDKEEENLVVALIEYLDKNAKGNIVIIDSLTVLA
                     QHCLERMEWKDLILFLRGLQKASKKWDGLVYTMLSEGIFERNKQIEIQDCMDGVIIFE
                     WDKQGISQRQRIMYMKKFRGLLPALDQDNIVNFETQISSQKGFEVSNIKRVRGR"
     gene            5727..7439
                     /locus_tag="VKL21_05420"
     CDS             5727..7439
                     /locus_tag="VKL21_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HMB45242.1"
                     /translation="MHIINEAKSILNYRDNNDNVIIDNVIIDNKIIDNEMESKKTLTE
                     KIEEEFADLKSYLHILTREKLKLEPYSHEKFGDIVDFGRLEGFVEKDRYWVNEPYAFI
                     SILYNEEKNEYQYQAVEPQLTAYEGMVLETVYENILDILTVYETSSQSKEEIIENKTI
                     ELIDSYSIEIDTKAIYKILYYIKRDYIGYERLNTLMKDPHIEDISCDGSDIPIFVYHR
                     KYHNLKTNISFEGKKLDSFVVKLTQRCGKQISIGEPLVNSTLPDGSRLNASLGKEVTF
                     RGSSFTIRKYREHAFTPADLINNGTYSPEILAYLWLVVENGKSMIFAGATAAGKTSSL
                     NSVSLFIPPVAKIVSIEDTHELMLHHANWIGSVTRESFAKTDTVCDIDMYELLRQALR
                     QRPEYIIVGEIRGKEGMTLFQAINTGHTTYSTMHADSVDTVISRLEGDPINIPRVMIQ
                     ALDILCIQKMIIRGDKRVRRLDSIVEFIGIDSKTQDLRFNELYTWDPKNDIFKNSGKS
                     FVLEDIMVSRNWDEKQLNTELENRKKVLEYMVEKEMDEYEIVSLIRSYYADAGKTMKL
                     IRNI"
     gene            7436..9286
                     /locus_tag="VKL21_05425"
     CDS             7436..9286
                     /locus_tag="VKL21_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMB45243.1"
                     /translation="MNRSNKLAFAIFGSFVRRNKERYFSLRSSIKQGHIAMPWDIYVS
                     SAYLYSIITGFIGVSFCLLLAPLWDILFSDFLITALIIVFISALVAMISYYLIIAYPG
                     LVAKVRKSKIDLTLPHGVAYMHAMSKGGLSLISIFRSLSQHSNIYGEFAEEIEYIVID
                     TEIHGNDIITSLKNAATNTQSEKFRNFLDNLINVAETSGDLDTFLGNMVSHYQSAAET
                     DQNMYLEMLGMLAETYITVFVAGPLFLITIVIVMGLMGPGNLLILKVLIYAVIPLSAA
                     AFCILLSVVSLGGDARLVKIYSVSRKIRHYDDVRTIFSNDDERLIRKLLRSLRWTGVI
                     ETLKDPLKIFFTDPVKAFYITIPAAFVYFVLSIYTQKITVDILDDSVIISVLILFVPF
                     LLFYEKQTRHIKAIEDSMPVFLRRMAAINDVGMPLSDAIRSIAKINLGVLSTEIKLMY
                     KDIVWSNSVLDALTKFERRVKTISISRIITLITKASESTGNIKETLRVAANEATLADK
                     LKTQKFTVLFSYLIVVYMSFAVFLLVLYVFATMFLPKIPDVSSIEGSGMLMINARKVE
                     YTLLFMHASVIQGFFSGIIVGQMMGESVYDGLKHSMIMMTIAYLFFVLFV"
     gene            9295..10656
                     /locus_tag="VKL21_05430"
     CDS             9295..10656
                     /locus_tag="VKL21_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45244.1"
                     /translation="MRFFKDEKAVSEVVGAMMILLILVLYLGIMQARQVPQWNEELEK
                     QQLDIVYDDFVTMRSNLEDVSQKNIPKTSIFHMGVKYPQRFMLSNPGPGASGILYTYP
                     LRINISYETTKGTKWKNYTSMGIIYEQNGISDSPRFIYENGLIMKEFADGFVFVVDDS
                     QFLTSKDNIFIPILNGPMNSGSSMEIESLNIAPILNDGFSQVKFSSMNATIETRYPKI
                     WNNISKESRPSGSNFTIENGTKCSYGNSSGYCLKLTFIPGYNVKKLNLPGNENQPSQK
                     LMYTGMVTFDNSFDNSLMNSRGPTGPDGQDMFENGQGRLDIPASNKSTQFIIQDITMA
                     RSSYSGHHDEYDSHENYIILRFNVTDITNLWMVEIKFSMLNDNTIKVISVEQKSPHIA
                     DNATYNIKYGIYKEFNNMDITNTREVNLTKYYRFFSKITSPNALKIDEIDQQILYANF
                     VIN"
     gene            10706..11389
                     /locus_tag="VKL21_05435"
     CDS             10706..11389
                     /locus_tag="VKL21_05435"
                     /inference="COORDINATES: protein motif:HMM:NF039237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HMB45245.1"
                     /translation="MKFVLVLVLLLVFLQGCISPAVKTGDPSRYELPGLANTTIFYLN
                     LSSVQVINNVVDRASVDYRIEDSIQTFRYPVAVDYSGNNVSVNVSMISIMARNYAHFN
                     FSSNFSGFVAFTRPGGQDFTHFPADNGTIRVVLPENFTAGTIFLGYVQPKPDNITKDS
                     SGREVLIWNNPQNKKIRVRYHQNDTPVMLMYLFGSLLICALIVRGYYYYSISTLKKKR
                     EMLEKFPRK"
     gene            complement(11397..12278)
                     /locus_tag="VKL21_05440"
     CDS             complement(11397..12278)
                     /locus_tag="VKL21_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB45246.1"
                     /translation="MNEIEVTGLRKEYGNFTAVESLDLNVRKGEIFGIVGPNGAGKTT
                     TLKMLSGLIIPTSGIIKIQGLDFGKNSRAIKQKLGFLPEESPLYEGMTANDYLMFFAE
                     IYGITKETASCRIRELLCALKLDAEDKKIGEMSKGMQRKVAIARALINNPEYLILDEM
                     TSGLDPTTSKYLSDFVLDLKKQGKTIIFSAHNLYQVESICDRVLMMNRGKEVSIGSMP
                     EIRKMCGAIEYSIEFRAKNDFDFAAVKNNGNFLAKTNNIDEFNRITGWVAKHNGQIIN
                     LKTVETSLEDIFLKLMN"
     gene            12330..14096
                     /locus_tag="VKL21_05445"
     CDS             12330..14096
                     /locus_tag="VKL21_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45247.1"
                     /translation="MRRILTIARWELRRTKSSFSLKTRLLSFIILILIGMTSFFAAQS
                     GLHINDNIYRVVVTDPSIARVLQSDDKFEVHVANEENARSLFENEGFDILINGAKIYY
                     HDTDKSISALDAIDKAVKKYDETRLLSYNDLNNTFPVWITIKNIVREQAFQAPSINAL
                     PQIEKSQEKKSSVASPAAQEQGIEKIPEKTTGNTPSIKEISEINKVSLISGQSLATPS
                     HFNPPVPFKSVVLSFIFIFPIYFIAQFFSSSIMDERIKRKGELLLVSPVNSYQIVLGK
                     LLPYLLGSMALVAIMTFKMGGSPWIILILLPVSLMFLSTSFLGAIIARSFKELSFVLI
                     FLSVFLSGYIFFPAMFANIHAISIISPITLVVRLLENETVSVSDYMFSTTPLYLLSIL
                     IFIFGIFIYREEDLFTQKSIKEKLLDSIQEFIKKVPSPLFSLSIALLPLVFSIQLMLI
                     VLMFNFPIRIGIFFFIIVAAFIEELVKSVGIYTVFSRKMADVTTGNALKFAILSGLGF
                     FAGEKLLLLVVIASIAGSAFGSVMGIGLLVFPLLLHVTSAAAASLSIRYLGHREYLAC
                     VVIASMVHASYNLYLVRSVVFG"
     gene            14089..15150
                     /locus_tag="VKL21_05450"
     CDS             14089..15150
                     /locus_tag="VKL21_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45248.1"
                     /translation="MVNTKVIVKKEIGSLLNEKTLLLAIIVQLLIASFSSLIVIGLAS
                     FFDPSSLSKYDSPRADVGIVGQGELARFIKDSQVNPHYYTDIVSARSDFDNNKIDAII
                     VIPKEDPGGNEIINLIIYLPKSDLRGTFVTLQLKKPLEEYEGYVRDIRGARIGFEPIR
                     LYVDNIPEKTSTYFEFIYGILIPLLVFTPVFISGGLIIDMLTEEYERKTMDLLLVSPV
                     SFSQIVNGKMITAIIIVPAQAFLWLLLLTLNGVVVHNIGFILLLCAIIAAIVVILGAM
                     IAVKYKKRMISQYLYSLVLILMFLMGYIFTDSPFNLVTRLSADSIGVQVLVYCGGYAA
                     AAIVLYAGMIRFQISGSDN"
BASE COUNT         4512 a         3217 c         3165 g         4613 t
ORIGIN      
        1 atctaaacca aattccctgg caaatgctgc aagggctcga acttcacggt attttgtttt
       61 ttcgcggttc aattcatatg tgacctgaat tatttctttg atcgcgagcc cctcacgtat
      121 cacgaaatcc acctcaaaac cttgtgtatt tttccagtaa tggatatcgt actgctgcgg
      181 catatctcgt tttaattcca gatagaccgc gttctcaaac aacctgccat agtctatcct
      241 gccgctcatg gagtacataa accccatatc gcccagatac gcttttctgg ggtattgcga
      301 gcggtctttt attgtatggg agaatatgct gacaaaaaag aaaagatacc cttcctgcga
      361 gtatttttca agatcaagca gactttcttt tccgatcttg tgacccactc ccttaaagaa
      421 gttcaagctt tttgatgctg aaaaataggg cgattcgatg atatattttc caaagagctc
      481 tactgttgtt atgctgttgt ccgacatttc tgctaagtca agtcctatta tgtccctgaa
      541 gtatgagttc aggatttgta tcttatcggt tttatcgtct gtcagcacaa cttcgggaaa
      601 tccgccaaat atgagatatt catcaagcgc ctgttctatt ctgcctattc ccgcaacggt
      661 gagttccact ttgtgccctt tgaaactcat gaattccctg aaagatagcg ggtacatttc
      721 ataatgaagc atccgtcccc gcagcggtct ggatgggatt acaagctttt tgcgggatga
      781 gctaacgatc aggtgcagcc ttccttttaa taattcatgg ttccttgcaa gccatccctc
      841 ccaatcatga accccagtga tctcatcaag caccaggaat cctgtgtctc caaaccattt
      901 gagcacatca tcaagcactg atatattatc cttgatcctg ctgtcctcaa ggttgatata
      961 cgctgtattt ttaccttttt tgacaagctc ctgttggagc attatcagaa cacttgattt
     1021 tcctgacctt cgtattcctg taatgccaat gatcgtgagg cgggaattcg atatcaaacc
     1081 actaagatca agcgctctct tcttcagcgc ctttttcccg gcgtatgttt tccattcttc
     1141 aaataccatt tctgttgggt tcattgaaat tccaatattg tttttgtaca gaacaatata
     1201 tatagatttc tgtttttatt cagaactatt tggtcactaa aagtttctgt ttggtcacag
     1261 caccggtata ccccgtacga tgtacgaagc ctgtatgtgg tggttttcag gattgtttct
     1321 catatcaatt taaggtgcag ggcttcagtg tggggcgact ataaaggtgc gctgcaagcc
     1381 ttaccgtgtc cctcattgca tttggatatt ttggaggcga ggattgattg tatcgcaaaa
     1441 ttaatagata gtaccaccat ttagacccca gatgaccata gaaatcctgg aatcatatca
     1501 gaatcatatc gcagaaaggc aaaaacaggg aattcccccc cttcccctga atgccgaaca
     1561 ggtaaaagcg ctaatcccat tgttttttga tcctccttcc ggctcggaag atcttctcct
     1621 ggaactcctt aagaatcata ttccacccgg tgttgaccct gctgcccttg aaaaagcaaa
     1681 gttcttaaga caaatcgccc tgcgtgagaa aagatcaaaa ctgctcctgc cgcatgaggc
     1741 ggtctccctg ttaggcttga tgaaaggcgg gtataatatc gagacgctca ttgaattgct
     1801 caaagataag gatcttgcgg accaggcagt taatgccctt tcaaatacta ttttgatatt
     1861 tggtttcttt gacaggataa aagagctttc cgccacaaat cccggggcaa aaaaggttct
     1921 tgaaagctgg gcaaaagctg attggcacaa aggcgaaatt ccccaaacga agaaagtgac
     1981 aatattcaaa atcccgggag aagtaaatac cgatgatttt tccccggcaa aacgtgccgg
     2041 aacgcgcgcc gatattccgc tacatgctct ctccatgttt gagaaccgat atccaggggc
     2101 gatcgatgaa ataatggccc tgaaaaataa agggtatccg atagcattcg ccgcagacgt
     2161 tgtggggaca gggagcagca ggaaatcagc ggcaaattct ctaatatggt ggattggaga
     2221 tgacattcca ggcatcccga ataaacgaaa aggtggaata attcttggaa gccagatagc
     2281 gccaattttt ttcaatacag cgcgtgacag cggtgcgatc ccgatcaaat gcgacgtttg
     2341 cgcgctcaca tcgggcgatg tagttacact ggatttcaaa cagggaaagg tgcttggtga
     2401 gaacaaagaa gagatcgctt cattttctct tgaaccccaa acgcttcctg atgaatacgg
     2461 ggccggagga aggctttcac tcataatagg aaaagaactc acaaaaaaag cacaggaagc
     2521 gcttggagta aattatgatc tattcatcaa gcccggggaa ccggcaactt cagatcgggg
     2581 atacactctc gctcaaaaga tagttggaaa agcatgcggg tatcctggtg tgcgccccgg
     2641 gcagtcatgc cttccctgta tcaccacagt gggttcgcag gatacgaccg ggccgatgac
     2701 cgttgatgaa ataaaagaac ttgtatgcct tggattccag gcgcctcttg ttatgcaatc
     2761 cttttgccat acagcggctt atccaacgac tgcggacacc ttaatgcata aagaactctc
     2821 tacattcttc aaggaaagag gtggagtagt tttaaaaccc ggcgacggaa taatccattc
     2881 atggctaaac cgcatgatcc tgcccgacac cgtgggtacg ggaggggata gccatacacg
     2941 cttcccgata ggcatctcat ttcccgccgg aagcggtctt gttgcatttg cggcctctct
     3001 tggtgtaatg ccacttgata tgccacaaag cgtgcttgta agatttaagg gcaaacttaa
     3061 aaaaggatta tacttacgtg atgttgtcaa tgcagttccg tattatgcgt tgaaacaggg
     3121 caagttaact cttgagaaaa agaataaaaa gaatatcttt aatggcagga taatggagat
     3181 ggaaggtctt cccgacatta ctgtagaaca ggcatttgaa ctcacggatg caagcgctga
     3241 gaggtcggta gaagctgccg tgatagccct gggaaaggag caggtagcaa tatttcttga
     3301 aaccaacata caaattttaa gatcgctcat taaggaagga tatgcatcaa atacacttga
     3361 aatgcggata aaggcaatgc aggaatggct tgatgctcct tttttgatgg aagccgatcc
     3421 tgatgcggaa tacgcagata tcctccagat cgatctatcc gatatcaatg aaccattcat
     3481 tgcctgcccc aatgatcccg attttgttaa accgctatct gaagtacaag gagtaaaaat
     3541 tgatgaagta tttatcggct cctgcatgat agggattgaa cagctggaca gggcggcaaa
     3601 aatacttgaa aaagcaggca gcatcaaagc gaaattgtgg gtggctcccc ccacaaggct
     3661 tatcgaaaaa accatgaaag aaaaaggaaa ttattcaagg ctcacaggac ttggggcaag
     3721 aatggaaatc cctgggtgca gcctttgcat gggcaaccag gcaagagtgg ctgataatgc
     3781 cattgtattc tcaacttcta cgcgcaattt cgataaccgc atgggtaaaa atgctcaggt
     3841 ctatcttggc tcggccgaac ttgctgcgat cacggctgtt cttggaagga tcccgacact
     3901 ttcggaatat cataagctat tgggatagca acgatgatta tttctaattt tattactttc
     3961 aagatgccct aaatgtgcaa actaattgga acgaggatct ccacgtatgg cgagagccat
     4021 atgtgtaggc gacgtcaact ggcgcaacgg ttgacgcgga tgcgtgcgga taaaattatc
     4081 aatgtccaat taaaaaggag atacaaaatg ttgtatccgg aattatcaga aatagaaacc
     4141 tcaaatccgt gtgcatctgc gtaatccgtg tcatccgcat tcctttaaga aaagacttca
     4201 gcctcagagt gtactattat cccatttttt gaaattacat aaggtttgat atccctggaa
     4261 tggtcaatgc gcctcatttt tgtgacttcg atgactgttg tcacggcccg aaggtcggtc
     4321 tgtcttacct gtcgtaatac gatgacccca tcagctatgt attctataag cccgaatttt
     4381 gaattatatg cattgttgtg gtgagtttct gaagtcatga ctaccgttat cccgtgttct
     4441 cttattttct ccgcgaaatt aaagaggtgt tcccggcgct ccgattcatt ttcatacagc
     4501 atttcataca atgtgatcgg gtcaataaca agccttttca cgccgaacga ctcaaaaagt
     4561 cttggcaggt cattttcaac ccgattggcc gtaattttaa tgttcatggc acttaaccgg
     4621 atcaatacta attttttatt ggtaatgtat ggcttaaggt cccagccgaa aatcgaagcg
     4681 gttttaacga tcgtctcctc gctttcctca aggctaaggt atatgcaatt ctcatctttc
     4741 aggagtcctg catatatgaa ttgcagggcg aaagtggatt tgcctgtacc cggtgatcca
     4801 agaactgcaa caatatgctc tttcgggatc cccccgcaaa gcatttcatc aagtccagtt
     4861 actcctgtat taagtgaatc cattatcttc ctcttactct tttaatattt gatacctcga
     4921 aacctttttg cgaacttatc tgtgtctcaa aatttacaat attatcctga tccaatgccg
     4981 ggagaagtcc cctgaatttt ttcatataca taatacgctg cctttgtgaa atcccctgtt
     5041 tatcccattc aaaaataatc accccgtcca tacaatcctg gatctcgatc tgtttgttcc
     5101 tctcaaatat cccttcgctt aacattgtat acacaaggcc atcccatttt tttgaagcct
     5161 tttgaagccc gcgcaaaaac aggatcaggt ctttccattc catcctttcc aggcaatgct
     5221 gggcaagtac tgttaaagaa tctattatta caatgttgcc tttagcattt ttatcaagat
     5281 attcgatgag tgcgacaacg aggttttctt cttccttatc ccacttcagg gaatcaaacg
     5341 aaagttcggt ctgggaagat gaacgccagt taataggaat aaaagatctt gcaaaaaaag
     5401 cctccgaaaa atctttgaat tcaaggcgat ccctgatcat attgtagaat tccggaaacg
     5461 agaatgcaac ctctttcata acatcatctt ttcctcgtgt aaatgaaaca taacacactt
     5521 tcccgggaaa taaattaccg gcctttggcc tgttaagcag ccgtgcgata gacgtgattg
     5581 caaattcgaa ctctcctgcg cctatctcac ccagcaataa cacgagtgaa cccgaaggaa
     5641 aaccgccctg gagaacaggg tcaagtgaat caataccggt gggatttttc tttaagtcca
     5701 tgaaatttgc atcctcaata ttttaaatgc atattatcaa tgaagcgaaa agtatattaa
     5761 attatcgtga taataatgat aatgtaataa ttgataatgt aataattgat aataaaataa
     5821 ttgataatga aatggaatcc aaaaaaaccc ttacagaaaa aattgaagaa gaatttgctg
     5881 acttgaagag ttatctccat attctcacca gggaaaaact aaagcttgaa ccttattccc
     5941 atgaaaaatt cggagatatt gttgattttg ggagattgga aggttttgtc gaaaaagacc
     6001 gatactgggt taatgaacct tatgcattta tttcaattct ttataatgaa gagaaaaatg
     6061 aataccaata ccaggcagta gagccgcaac ttactgctta tgaagggatg gtgcttgaga
     6121 cggtctatga aaacattctt gatattctta ctgtttatga aacctcaagc cagtcaaaag
     6181 aggagatcat agaaaacaaa acgattgagc tgatcgacag ttattcaata gaaatagata
     6241 caaaagccat ctataagatc ctctactata tcaaaaggga ttatatcggt tatgagaggc
     6301 tgaacaccct catgaaagat ccgcacatag aagatatatc atgtgacggc tcggatatcc
     6361 cgatatttgt gtatcacagg aaataccata atcttaagac caatatatcc ttcgaaggaa
     6421 aaaagcttga ttcgttcgtc gtcaagctta cacaaagatg cggcaagcag atatcaattg
     6481 gagaacccct ggtaaacagc acactccctg atggctctcg actgaatgct tctcttggaa
     6541 aagaagttac cttcagggga agctcattta ccatcaggaa ataccgtgaa catgcgttta
     6601 cacctgctga tcttatcaac aatggcactt attctccaga aatacttgct tatctgtggc
     6661 ttgttgttga aaatggcaag agcatgatat ttgcaggtgc gacagcggcc gggaaaacat
     6721 catcgttgaa ttctgtttcc ctgtttattc ctcctgttgc caaaatcgtc agcatcgaag
     6781 atacgcatga actcatgctg caccatgcca actggatcgg aagcgtcaca cgtgaatcat
     6841 ttgcaaagac agatactgtc tgcgatattg atatgtatga actcttgcgc caggcgcttc
     6901 gacagcgtcc cgaatatata attgtggggg agatcagggg aaaagaaggc atgacactgt
     6961 tccaggctat aaatactggc cataccacat attcgacaat gcatgcagat tctgtagata
     7021 cggttatcag ccggcttgaa ggcgacccga taaatatccc gcgcgtaatg atccaggcgc
     7081 ttgatatcct ttgtatccag aagatgataa ttcgtggtga caagagagta cggcgccttg
     7141 acagcatagt agaattcata ggaattgatt ccaaaaccca ggacctcagg ttcaatgaac
     7201 tgtatacctg ggatccgaaa aatgatattt tcaagaatag cggaaaatcg ttcgttcttg
     7261 aagatatcat ggttagccgg aactgggatg aaaaacagtt gaacacggaa cttgagaaca
     7321 ggaagaaagt ccttgaatat atggttgaaa aggaaatgga tgagtacgaa atcgtttctc
     7381 ttatccgcag ctattatgca gatgccggga aaaccatgaa attaatcagg aatatatgaa
     7441 tagatcaaat aaactggcat ttgccatttt cggaagtttt gtacgaagaa ataaagagag
     7501 gtatttttcc ctcaggtcat cgatcaaaca ggggcatatc gcaatgccct gggacatata
     7561 tgtgagcagt gcatatcttt attccataat aacaggtttc attggcgttt cattctgtct
     7621 attacttgca cctttgtggg atattttatt ttcagatttt ttgatcactg cattgataat
     7681 tgtattcatt tcagcgttgg tggcaatgat ctcatattat cttattattg catatccggg
     7741 actggtggca aaagtgagaa aaagcaagat agaccttacg ctcccccatg gcgtcgccta
     7801 tatgcatgca atgagcaaag gcggtttaag tcttatttca atatttagat ctttgagcca
     7861 gcatagcaat atatatggcg aatttgcaga agagatcgaa tacattgtta tagataccga
     7921 aatacacggc aatgatatta ttacttcact gaaaaatgct gccaccaata cccaatctga
     7981 gaagttcagg aatttcctgg ataatctcat taatgtggca gaaacaagcg gtgaccttga
     8041 tacatttctc ggtaatatgg tgtcccatta ccagagtgcg gcagagacgg accagaacat
     8101 gtatcttgag atgcttggta tgcttgccga gacatatatc acggttttcg ttgccggccc
     8161 ccttttcttg ataaccattg tcatagtcat ggggcttatg ggtccgggaa atctgctaat
     8221 actcaaggtt cttatatatg ctgtaattcc attaagcgct gctgcatttt gtattcttct
     8281 aagcgtagtt tccctgggcg gcgatgccag gcttgttaaa atatattctg tttccagaaa
     8341 gataaggcat tacgacgatg tcagaacaat cttttcaaat gatgacgaga gactgatacg
     8401 caaacttctg aggtctttga ggtggacagg cgtgatagag acattaaaag atcctttaaa
     8461 aatatttttt accgatccgg taaaagcgtt ctatatcaca attcctgcgg catttgttta
     8521 ttttgtctta tccatataca cacaaaagat aacggttgat atcctggatg actccgtgat
     8581 aataagcgtg ttgatactgt ttgtaccgtt cctgttattt tatgaaaaac agacgcgaca
     8641 tattaaagca attgaagact caatgcctgt tttcttaagg cggatggctg ctattaacga
     8701 tgtcggtatg ccactttctg atgccataag atccattgcc aaaataaacc tgggtgtttt
     8761 gagcactgaa ataaaactca tgtacaaaga tattgtctgg agcaacagcg ttcttgacgc
     8821 gctcacaaaa tttgaacgaa gggtcaaaac aatttctatt tcaaggatca tcaccctgat
     8881 cacaaaggca agtgagtcca caggtaatat caaggaaaca cttcgggttg cagcaaatga
     8941 agcaacactt gctgataaac tcaagacgca gaaattcacc gtcctgttct cttaccttat
     9001 tgttgtatat atgtcgtttg cggtattcct tctggtatta tatgtatttg caacgatgtt
     9061 ccttccaaag atacctgatg tgagttccat agaaggttcg ggaatgctca tgataaatgc
     9121 ccgcaaggtt gaatatactc ttttattcat gcacgcatct gtgatacagg gtttcttttc
     9181 aggcatcatc gtgggccaga tgatgggaga aagcgtatac gatggtctta aacattcgat
     9241 gatcatgatg actatagcct atcttttttt tgtcctgttc gtgtagagga aaatatgagg
     9301 ttttttaaag atgaaaaagc ggtttcggaa gtggttggcg cgatgatgat acttttgatc
     9361 ctggtgcttt atcttgggat catgcaggca cgccaggtcc cccagtggaa tgaagaattg
     9421 gaaaaacagc agttggatat tgtatatgat gattttgtta ccatgcgctc aaacctggaa
     9481 gatgtttcgc aaaagaatat tcccaaaaca agtatttttc atatgggggt taagtatccg
     9541 cagcgcttta tgctttcaaa tccgggaccc ggggcaagcg ggatactcta cacatatcca
     9601 ttgaggatca atatcagtta tgaaactacg aaaggcacta agtggaaaaa ttacacctca
     9661 atgggaatta tctatgaaca gaatggtatt tcagattcgc ccaggtttat atatgaaaat
     9721 ggtttaatta tgaaagagtt tgcagatgga tttgtttttg tagtagatga tagccaattt
     9781 ttaacctcaa aagataacat atttatccct attttaaatg gacctatgaa ctccggttca
     9841 tctatggaaa ttgaatcact gaatattgcg ccaatattaa atgatggttt ttcacaggta
     9901 aaattttcat cgatgaatgc aactatagaa acacgatacc cgaaaatctg gaataacata
     9961 tctaaagaat caagaccatc cggctccaat tttacaatag agaacggaac aaagtgctct
    10021 tatggaaatt cttccggtta ttgtttaaaa ttaacattca tccccgggta taatgtgaaa
    10081 aaactcaatt taccgggtaa tgaaaaccaa ccttcacaga aactgatgta tacgggaatg
    10141 gtcacttttg ataactcttt tgataactca ttaatgaatt caagagggcc cacaggacct
    10201 gacggacagg atatgttcga gaatgggcag ggacggcttg atattccggc aagtaataaa
    10261 tctacccaat tcattatcca ggatattaca atggcacgtt cgagttatag cgggcatcat
    10321 gatgaatatg attcgcatga aaattatata atattgagat tcaatgtaac agatataaca
    10381 aatctatgga tggttgagat aaaattttcc atgttaaatg ataatacaat aaaagtaata
    10441 tccgtagaac aaaaatctcc tcacattgca gacaatgcaa catataatat taaatatgga
    10501 atatataaag aattcaataa tatggatatt acaaatacaa gagaggttaa cctgacgaag
    10561 tactaccggt ttttctcaaa gatcacttct cctaatgctt tgaagattga tgaaatagat
    10621 caacaaattt tgtatgccaa tttcgtcatt aactgaattt ccaaaggata atctttaagc
    10681 acaatatcga tatagttctg catcaatgaa attcgttctg gtcttggtct tgcttttggt
    10741 ttttttgcaa ggctgcattt cccctgctgt taaaacaggc gatccttccc gttatgaact
    10801 acccggtctt gccaatacca ctatttttta cctgaatctc tcttctgttc aagtaataaa
    10861 caatgttgtt gacagggcat ctgttgatta cagaatagaa gacagcatcc agactttcag
    10921 gtatcctgtt gcagtagatt attcaggcaa taatgtcagc gtgaatgttt caatgatttc
    10981 aattatggca agaaattatg cacattttaa tttcagctcg aatttttcag gatttgtggc
    11041 ttttaccagg ccaggaggac aggattttac acattttccg gcagacaatg gcacaattcg
    11101 cgtggtcttg ccggaaaatt tcacagcagg aacaatattc cttggatatg tccagccaaa
    11161 acccgataat attacaaagg actcatcagg aagagaagtt cttatctgga ataacccaca
    11221 aaataaaaag atcagggtaa gatatcacca aaatgatacc ccggtaatgt taatgtattt
    11281 attcggatca ctattgatct gtgccctgat tgtccgggga tattattact acagtatctc
    11341 tacccttaag aagaaacgcg aaatgctgga aaaattccct cgaaagtgaa tttttttcaa
    11401 ttcatcagtt taaggaaaat atcttcaagt gaagtctcca ctgtttttaa atttattatt
    11461 tgtccgttgt gctttgccac ccaacctgta atcctgttaa attcatcaat attatttgtt
    11521 tttgccagga aattcccatt atttttcact gcagcaaaat caaagtcatt ctttgcacga
    11581 aactcgatcg aatattcaat agctccgcac attttcctta tttcgggcat gctgccgata
    11641 gaaacttctt ttccccggtt catcatgaga acacggtcac atatgctttc aacctggtac
    11701 aggttatgcg cgctaaatat tatggttttt ccctgttttt tgaggtctaa tacaaaatcc
    11761 gaaagatatt tggatgttgt gggatcaaga cctgaggtca tctcatccag gatgaggtat
    11821 tcggggttat ttatcaatgc ccttgctatt gccacctttc gttgcattcc ctttgacatc
    11881 tcgccaattt ttttgtcctc cgcatcaagt ttaagggcac aaaggagttc cctgatccgg
    11941 caagatgcag tttcttttgt tataccgtat atttctgcga aaaacatgag gtaatcattt
    12001 gcagtcatcc cttcatataa cggactttcc tcaggcaaga aaccaagttt ctgttttatt
    12061 gcccgggaat ttttcccaaa atccaatccc tgtattttta ttatcccgct ggtgggaata
    12121 attaatccgc tcaacatttt aagagtagtg gtctttcctg ctccgtttgg tcccacgata
    12181 ccgaaaatct cgccttttcg gacattaagg tccagggact cgacagctgt gaagttgcca
    12241 tattctttcc tgagtcctgt tacttcaatt tcattcatag aaatacttat ttatcttatg
    12301 agtataataa acattatgat ggaagaaata tgaggcgaat attaacaata gccagatggg
    12361 aattaaggag gaccaaatca agtttcagcc ttaagacccg tttactttct ttcataatat
    12421 tgatcttgat cggtatgact tctttttttg ccgcccagtc cggccttcat ataaatgata
    12481 atatttacag agtagtagta acagatccat ccattgccag ggttttgcaa agcgatgata
    12541 aattcgaagt tcacgtggca aatgaagaaa atgcaagatc tctttttgaa aacgaaggat
    12601 ttgatattct cataaacggg gcaaagatct actaccatga cactgataaa tcgatatccg
    12661 ccctggatgc catagacaaa gcagtaaaaa aatacgatga aacgaggctt ttatcctata
    12721 atgacctgaa taatactttt ccagtgtgga tcactattaa gaatattgtc cgggaacagg
    12781 cttttcaggc gccgtcgatc aatgcactac cacagatcga aaaatcacag gaaaaaaaat
    12841 catccgtggc atcaccagcc gcgcaggaac agggaatcga aaaaatccct gaaaaaacaa
    12901 ctggaaatac gccttcaatt aaagagattt ctgaaataaa taaagtctca ctcatcagcg
    12961 ggcaatcact tgcaactccg tctcatttta atccgcccgt tccttttaaa tcggttgttc
    13021 tcagtttcat atttatcttt cctatttatt ttatcgcgca gtttttttct tcaagcataa
    13081 tggatgagag aataaagaga aaaggtgaac ttttgctcgt atcaccggtc aattcttacc
    13141 agatcgtcct tggcaaactt ttgccgtatc ttctgggttc aatggcgctt gtggcaataa
    13201 tgacatttaa aatgggagga agcccctgga taatcctgat cttactgcct gtttccctga
    13261 tgttcctctc aacgtctttc ctgggggcaa taattgcgcg aagtttcaaa gaactcagtt
    13321 ttgtgctgat cttcctttcg gttttcctat caggctatat cttctttcct gcgatgttcg
    13381 caaatatcca tgccataagc atcatttccc cgataaccct tgttgtcagg ctgcttgaga
    13441 atgagaccgt ttctgtaagc gattatatgt tctccaccac tccattgtac ctgttatcta
    13501 tcctgatatt catattcggg attttcattt acagggaaga agacctgttc acgcaaaagt
    13561 ccatcaaaga aaaactcctg gattcgatcc aggaattcat taaaaaagtt ccatccccgc
    13621 ttttctccct gagtatcgca cttttgcctc ttgtattttc aattcagtta atgcttatcg
    13681 tcctgatgtt caattttcct atccgcatcg ggatattctt ttttattatt gtcgctgcgt
    13741 tcattgaaga acttgtaaaa tcagtgggga tatatacggt cttttcacga aaaatggctg
    13801 atgttacaac cggcaatgcc ctgaaattcg caatattatc aggccttggt ttttttgcgg
    13861 gtgaaaagct tctgcttctt gtggtcattg ccagtatcgc cggctcggca ttcgggtcag
    13921 tgatggggat agggcttctt gtttttccat tgctcctgca tgtaacttca gcagcagcgg
    13981 cctctttgag catccgatac ctggggcatc gggagtatct ggcttgcgtt gtcatagcat
    14041 cgatggtcca tgcgagctat aatctttacc tggtaaggag tgttgttttt ggttaataca
    14101 aaagtcatag taaaaaagga gataggatcc ctcctgaatg agaaaacact tttgcttgca
    14161 attatagtcc aattgttaat cgcttccttc tcatctttga tcgtaatagg gcttgcttcc
    14221 ttttttgatc cctcctctct tagcaaatat gacagtccca gggcagatgt gggaattgtg
    14281 ggacagggag aactggcacg attcatcaag gatagccagg taaatccgca ttattatacg
    14341 gacattgttt ctgcacgttc tgattttgac aataataaaa ttgatgcgat aatcgtcatc
    14401 ccgaaagagg acccgggagg aaatgaaatc atcaatctga tcatctatct tccaaaatcg
    14461 gatttaaggg gcactttcgt aacgctgcaa ctgaaaaaac ccctggagga atatgaaggc
    14521 tatgtccgcg atataagggg agcaagaatt ggttttgaac ccataagact ctatgttgat
    14581 aatattcctg aaaaaacctc cacctatttt gaatttatat acgggatact tatcccgctt
    14641 ctcgttttca cgccggtttt catttccggc ggtttgataa tcgatatgct caccgaggaa
    14701 tacgaacgta agaccatgga cttgctgctg gtttccccgg tgtcattttc acaaatagtg
    14761 aacggaaaga tgatcacggc cataattata gtccctgcgc aggcattttt atggcttctt
    14821 cttttgacgt taaatggagt tgtggttcat aacatcggat tcatcctgct cctgtgcgca
    14881 attatagcag cgatcgttgt tattcttgga gcaatgatag cagtcaaata taaaaagagg
    14941 atgatctcac agtatttgta ttcactcgtg cttatattga tgttcctcat gggttatatt
    15001 ttcacggatt ctccttttaa cctggtaacc aggctcagcg ccgattctat tggggtgcag
    15061 gtgcttgttt attgcggagg gtatgcggcg gctgcaattg tgctgtatgc gggtatgatt
    15121 agatttcaaa tttccgggtc ggataactga agaaaaaaag aacaacgaac tggtacgaac
    15181 tccggtcgtc gcgagttcgt tgtaaatttc ttcaatattt ataacataca cattaattat
    15241 ctactgatct ttaagaatct gcttctgtgc gctcatggca agcatggagt taataagctt
    15301 catcccttcc tctatatcat ttaccctgag cgttactttt ccatcagcaa aaagtcaagt
    15361 tttatccttg caccgtcgcc tcctgcaccc tgcggcacag gttcggtgta tcattggctt
    15421 gaccttcatt gctactatgg gttcgtccga cttctcataa cgcattgctt cggttttccc
    15481 atcaggttat tcctatgcta cctgttt
//
LOCUS       DATGBN010000227         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_367341, whole genome shotgun sequence.
ACCESSION   DATGBN010000227 DATGBN010000000
VERSION     DATGBN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367341"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..462)
                     /locus_tag="VKL21_05455"
     CDS             complement(199..462)
                     /locus_tag="VKL21_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45249.1"
                     /translation="MEIEPIEFKEFLKGSFEKIEEIGGEVSLGYVKNGVWNEYQKEQN
                     WNTVKAVFPGKEPKFRSIESFLNEYNEYVEKFYKRQESIASGF"
     gene            complement(1021..1266)
                     /locus_tag="VKL21_05460"
     CDS             complement(1021..1266)
                     /locus_tag="VKL21_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45250.1"
                     /translation="MRMTMIEALEKLAELNDYNEILVQNNAGELKAIDFAIDDIRNYA
                     DDEDEIYDLEVTEETIREFDEFGYIKSGEPLYKVVKE"
     gene            complement(1263..1499)
                     /locus_tag="VKL21_05465"
     CDS             complement(1263..1499)
                     /locus_tag="VKL21_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45251.1"
                     /translation="MTEPKRRQGQKKPNGRIAIPKPICPECGEMMIRTYTRGNAERKR
                     AYVPAGWSCPSSTCYYIEKDYVELEDTEEGEAEI"
BASE COUNT          482 a          398 c          288 g          690 t
ORIGIN      
        1 aaattgacca aacctcataa atttcttttt gtcagtagca aaagcagtct taagatattc
       61 cttttcacat ttcgggcaaa cagttttagg gactgctccg ccctgccctt gtcttcgatt
      121 tttttcagtt atgctttaac ctcttttcgg ttcaattagg agcgcaacta atttggagta
      181 tttagagaat aaaggggatt agaaccccga tgcaattgat tcttgtcttt tgtagaactt
      241 ttctacatat tcattgtatt cattcaagaa tgattcaata cttctgaatt tcggttcttt
      301 acccggaaat actgctttta cggtgttcca gttctgttct ttttggtatt cgttccagac
      361 accgtttttc acataaccaa gtgagacttc cccgccaatt tcttcaattt tctcgaaaga
      421 ccctttgaga aattccttaa attcgatagg ttctatttcc atgattctgt ttctccaaga
      481 ttcagatcct ttctgtagtc ttcctacttt tgtgcaagca tttgatttca atattctgta
      541 tctctgtaac tcattttgtt gcgcttctat tattttgcta tattctatag tttccagtta
      601 tgcatatata tcctttttct tcattctata ggacgtagta aaataataat aaaaccgctg
      661 tcaaaagaac gaacgattcc aagccctttt gtgagccttt taaaaaagca caggagatat
      721 gaacctatca atgagttcgc aattgtcaag caggacgccg gaaaacagtt taatctctaa
      781 tcagtagcaa aaaataccga aagttttata tcacgattaa tcatttaata tattgggtaa
      841 aataagtggc caaagcttca tattcagggc agtttaacct cctttctgcc ctgatatctc
      901 ttttaaatca attatcctta ttaggataag ccgcctacga ctgattaata cttgattaac
      961 agctcatccg gcagctgtca ggctttatta ttaacacaaa aaagaaaaag taaattaaaa
     1021 ctactctttt acgactttat ataacggttc accgcttttt atataaccaa attcatcaaa
     1081 ctctctgatg gtctcttccg tgacttcgag atcataaatt tcatcctcgt cgtctgcgta
     1141 gttcctaatg tcatcaatag caaaatcaat tgcttttagt tctcctgcgt tgttctgcac
     1201 aaggatttcg ttgtaatcat taagttctgc aagtttttca agagcttcaa tcattgtcat
     1261 tctcatattt ccgcttctcc ttcctctgta tcttccaatt ccacatagtc tttttcaata
     1321 tagtaacagg tagaagaggg acagctccac cccgccggca cataagcccg ctttctctct
     1381 gcgtttcctc ttgtgtatgt tcgtatcatc atttctccac attcaggaca aatcggttta
     1441 gggattgcta tccgtccatt gggttttttc tgaccttgtc ttctttttgg ttcagtcatt
     1501 gtttcacctt cgtctttaat tcggagcgca acatatctat ttttcgaaaa atgattaagg
     1561 gtaaaatttt gagagatatt cctcttgata ttccctttca aagtcctcca gagttctcag
     1621 cattgggact tgttcaggcc attggatccg gaccaagcca ggacatagga aagtaaattc
     1681 tgcccctttt ttctcgcact ttctcatgca tatcaggaac catttatcaa attccactgg
     1741 atttgttttt tgtacttccc gtttgttcca ttgtctgtgt tgaacactga tgttttgttt
     1801 ctatgctgtt cctattttca ttttgttgcg ctcctttatc ttactgtaat atactata
//
LOCUS       DATGBN010000228         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_561036, whole genome shotgun sequence.
ACCESSION   DATGBN010000228 DATGBN010000000
VERSION     DATGBN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561036"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VKL21_05470"
     CDS             complement(<1..343)
                     /locus_tag="VKL21_05470"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HMB45252.1"
                     /translation="MEEKKLGNVTEKKKTSMGLDENVAGALCYVLGFLTGIVFFVMEK
                     DSKFVKFHAVQSIATFLPLMILGWIISSLFWSFTMMWLVGILSTMLFIIELVLWIILI
                     MKAYQGEMYKLP"
     gene            complement(371..748)
                     /locus_tag="VKL21_05475"
     CDS             complement(371..748)
                     /locus_tag="VKL21_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45253.1"
                     /translation="MKSNKSTKILIILSIIGLIAFAGCTGKQDTITTPDGDVKISEGG
                     SGPDWCKTGTKMTTTGADGKQGYFEIKGSTNYEGKEVCESQWSSDDGSVTQYFNEDSS
                     YLVIITKDKSGKEINKVVNSQPK"
     gene            1000..1806
                     /locus_tag="VKL21_05480"
     CDS             1000..1806
                     /locus_tag="VKL21_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HMB45254.1"
                     /translation="MIIGASSFAGPLPELESEVESIELYIPKLEVYEGTKLIRSRISK
                     LKDTLSSYDLSTSIHAPYFSDAPNYPHELIVDTARLTDKTRRLLKESIGIAEGLGSGI
                     VVIHPGRITGVRERCIESMISGLSRLSKFAEDANVTLGLENKEGTDQNNLCISASDLI
                     KSIEEIGSPNLRATFDIGHANLTCEGNQGKLREYAKELKNHIAHIHIHDNPGFLTEKF
                     WGDLHGAPGTGVIDFSILKDLRFKGVHNLEVFSLEDVRSGKKMLLGLNDE"
     gene            complement(1814..2101)
                     /locus_tag="VKL21_05485"
     CDS             complement(1814..2101)
                     /locus_tag="VKL21_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45255.1"
                     /translation="MDINCIMAIGLGDIYDKLKELITIVDKVRVNTEEIKELKNSQIE
                     IKKNQEEMQGNILKLMEGQKHILERLEYKEELSSLKIKLAEHIAACEKAKA"
     gene            2204..>3944
                     /locus_tag="VKL21_05490"
     CDS             2204..>3944
                     /locus_tag="VKL21_05490"
                     /inference="COORDINATES: protein motif:HMM:NF010368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HMB45256.1"
                     /translation="MPYYLGELLLYWCHSCNLPVLGKTCACGLATKKIEITPPGDIRP
                     AFPYDIDLINRTTEKQFGLKLIPEGHLAVLNKAPYEDRMDEVIFDGAIMGSIRFELEK
                     MDWVFIPRLEGARRLVNGTKCIIIDKGAVNYIIKGASVLAPGVNDANLGIVEDDEVII
                     LTPDNEVIATGRARMNASRMLTHEKGMAVKTRWHDKPHVMEQSRDQEKTWDDAVQANI
                     QILRTKEEKAHSFIKNVVETSKRPVTVSYSGGKDSLVTLLLVRDTLTGFDILFADTGL
                     EFPETIDNVKQTANCLSLPLKIHSSGDSFWQSIDNFGPPTVEARWCCKVCKLGAITQV
                     IEQNYENGCLTFIGQRKYESEIRANSEHIWKNPSVGNQIGATPIQNWTALHVWLYLFW
                     KKAKYNPLYEEGFDRIGCWLCPSASMADFSRLSQIHPELSEKLEKYLNDYASRNGLSR
                     EWVKYGFWRWQVLPKSIGMIAQKLGINIIPKSEKKPVQFTLTVGYRPCKAGGITAEGS
                     FGVPLNLSKIEESGFMGMIGETNSMEGVILASRNENNVHVYASGTVVARGGNEDEARE
                     LIQLAQMTIRRAVL"
BASE COUNT         1174 a          781 c          826 g         1163 t
ORIGIN      
        1 ttggcaattt gtacatctct ccctgatatg ctttcattat taaaatgatc catagaacca
       61 gctctatgat aaagagcatt gtcgataaaa ttcctactaa ccacatcatt gtaaaggacc
      121 agaataatga tgaaataatc catccaagta tcatcaaggg cagaaatgtt gctatagatt
      181 gtactgcgtg gaatttcacg aatttgctat ctttttccat tacaaagaaa acgattccag
      241 ttaaaaatcc aagaacgtaa cacaatgcac ctgcaacgtt ctcgtccaat cccattgaag
      301 tcttcttctt ttcagttaca ttacccagtt ttttttcttc cataatattt tctccttata
      361 ttgattttgt ttactttggt tgtgaattaa caaccttatt gatttctttt ccggatttat
      421 ctttagtgat aattacaagg taactggagt cttcattgaa gtattgcgtc acagaaccat
      481 catcactgct ccactgggat tcgcagactt ctttgccttc ataattggta cttcctttta
      541 tctcaaaata tccctgtttt ccatcagcgc ctgtggtggt catttttgtt cctgttttac
      601 accaatcggg tcctgatcca ccttctgata tctttacatc tccatctgga gttgtgattg
      661 tatcttgttt tcctgtacat ccagcaaatg ctatcagtcc tattattgat agaattataa
      721 ggattttcgt gcttttgtta gatttcatgt ttttcaccta ttttatgtca tccacatgga
      781 attgcatgat ttttcgttat aaaccgtgtt aatatttcaa tatatggtat ttaaatatat
      841 ctacaaagaa tttattcgaa taactacgaa cataaattga aattataggg tacaaacagg
      901 aaaaacatga cctgcatata ttttatatgg gttaaattta atactttttg ctggaatgtt
      961 aagctattct taaagaaaac aaaaatattc ttgtgacata tgatcatcgg agcatcgtct
     1021 tttgcagggc cacttcctga attggagtct gaagttgaat cgatagagct gtatatacca
     1081 aaactggaag tctatgaagg aacaaaatta ataagatcgc ggataagcaa gctaaaagac
     1141 acgctttcat catacgattt atcaacttca atccatgctc cttatttcag tgatgcgcca
     1201 aattatccgc atgaacttat tgtggatact gccaggttga ctgataaaac ccggaggctt
     1261 ttgaaggaaa gtataggaat agcagaaggt cttgggtcag ggatcgtggt cattcatccg
     1321 ggaaggataa ccggggttag agagagatgc attgaaagca tgatatccgg attgtcccgc
     1381 ctttcgaaat ttgccgaaga tgcaaatgtt acgctcgggc ttgaaaataa ggaagggaca
     1441 gaccaaaaca acctgtgcat aagcgcttct gatcttataa aatctattga agaaataggc
     1501 tcccctaatt tgagagccac atttgatatt ggacatgcaa atctgacctg cgaggggaac
     1561 cagggtaaac ttcgggaata tgcaaaggaa ctaaaaaatc atatagcaca tattcatatc
     1621 cacgataatc ccggtttcct gaccgaaaag ttctggggcg accttcatgg cgctccgggg
     1681 actggtgtca ttgatttttc catcctgaaa gatctcaggt tcaagggggt tcataacctt
     1741 gaggtgttct cattagaaga tgtgagatcg ggcaagaaga tgcttttggg tttgaatgat
     1801 gaatgaagta agattatgct tttgcctttt cacacgctgc aatatgttca gcgagtttaa
     1861 tttttaatga ggatagttct tctttatatt caagtctttc taatatatgc ttctggcctt
     1921 ccatgagttt gagaatgttc ccctgcatct cttcctggtt cttttttatt tctatctgac
     1981 tatttttaag ttccttgatt tcttcagtat tcactctgac cttatctaca atagtaatta
     2041 gttctttaag tttatcgtaa atatcgccaa gtccgatagc cattatgcaa ttaatatcca
     2101 tttttatact taaaactttt tgggtatcaa agcatcaaga aattccttga atgatttaca
     2161 ggtgtcacca tacatatata cttccatcac taagtcagga accatgcctt attatctcgg
     2221 cgaactcctg ttatactggt gtcactcctg caatcttccc gttcttggaa aaacctgcgc
     2281 ctgcggtctg gccacaaaga agatcgaaat aacacctcct ggtgatataa ggcccgcatt
     2341 cccttatgat atagacctta tcaacaggac aacagagaaa caattcggcc tcaaactcat
     2401 ccctgaaggt catcttgcgg tactgaacaa ggccccttat gaagacagga tggatgaagt
     2461 gatatttgac ggcgccatca tgggttctat ccgctttgag cttgagaaaa tggattgggt
     2521 attcatcccc aggcttgagg gagcaaggcg ccttgtaaat gggacaaaat gcataattat
     2581 cgacaaaggc gctgtcaatt atattattaa aggcgcttct gtgcttgcac ccggtgtcaa
     2641 tgatgcaaat ctgggtattg ttgaggatga cgaagtaatt atcctgactc ctgataatga
     2701 agtgatcgca accggaaggg caaggatgaa cgcatccagg atgctcaccc atgaaaaggg
     2761 catggccgtg aaaacacgct ggcatgataa accgcatgtt atggagcaat ccagggatca
     2821 ggaaaaaaca tgggacgatg cagtacaggc gaacattcaa attctgcgga caaaggaaga
     2881 aaaagctcat agtttcataa aaaatgtagt ggaaacttca aaaagacctg tgaccgtttc
     2941 gtattccgga ggaaaggaca gtcttgtaac cctgcttctg gtgcgtgaca ccttaacagg
     3001 atttgatatc ctgttcgctg atacgggtct ggaattcccc gaaaccatag ataatgtgaa
     3061 acaaactgca aattgccttt ctttgccatt aaaaattcat tcatcgggtg attccttctg
     3121 gcaatccata gataatttcg gccctccgac cgtggaagca agatggtgct gcaaagtctg
     3181 taagctcggg gcgataacac aggtcatcga acaaaactat gagaatggat gcctcacatt
     3241 cattgggcag cgaaaatacg aaagcgagat acgggcgaac agtgaacaca tctggaaaaa
     3301 tccttctgtg ggaaaccaga taggagccac tcctatccag aattggaccg cgcttcatgt
     3361 ctggctgtac cttttctgga aaaaagcaaa atacaatcct ctctatgaag aaggatttga
     3421 caggatagga tgctggcttt gcccttcagc aagcatggct gatttctcgc gtctgtctca
     3481 aatacatcct gaattgagtg aaaagcttga aaaatacctg aacgattatg cttcccgtaa
     3541 tggtttatcc agggaatggg taaaatatgg cttctggcgc tggcaggtac ttccaaaatc
     3601 aatagggatg atcgcgcaaa agctgggaat aaacattatc ccgaagagtg aaaaaaaacc
     3661 ggtccaattc acattgactg tgggataccg tccctgcaaa gcaggaggca tcacggcaga
     3721 aggaagtttc ggggtcccat tgaatctttc aaagatcgag gaaagcggtt ttatgggcat
     3781 gatcggtgaa acaaattcga tggagggtgt gatccttgct tcgcgaaatg aaaataatgt
     3841 ccatgtttac gcaagcggga cagtggttgc aagaggaggg aatgaggatg aggccaggga
     3901 attgatacag ctagcgcaaa tgacgatacg tcgggcagta ttat
//
LOCUS       DATGBN010000229         5911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_367499, whole genome shotgun sequence.
ACCESSION   DATGBN010000229 DATGBN010000000
VERSION     DATGBN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367499"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VKL21_05495"
     CDS             complement(<1..314)
                     /locus_tag="VKL21_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HMB45257.1"
                     /translation="MSKKTAVIKGDGVGPELVNSMLRVAKAAGTKVEFILCEGGEQWW
                     KENGGDSLIPQETWDILGKTDAGFKGPTTTPGGAGSPKSVAVSIRQKFNLYANVRPIK
                     SFP"
     gene            complement(323..1105)
                     /locus_tag="VKL21_05500"
     CDS             complement(323..1105)
                     /locus_tag="VKL21_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45258.1"
                     /translation="MDYEKMELQTHRSNSGYIMEADINVIQAAASRGLAWLQEQEPQT
                     VKDISRSIQALSLWDESASILIEKLISMKKDGYWETQTPINDTARACIALLGCEKVHV
                     EIIRWIQEQQRGENWNNNEIDTSYALMALGDREIKNIPGCEWLIRNYGPKWEHAGTTS
                     LIITALIKQDREEYRDFIKERASWLISKREDSGWTYIATSNLVIQALILAGTKEEDIE
                     PSIKWLLGKQQENGSWKDIISTTLSLISLKMYLDKLNSISDE"
     gene            1257..1814
                     /locus_tag="VKL21_05505"
     CDS             1257..1814
                     /locus_tag="VKL21_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HMB45259.1"
                     /translation="MAQSVFRKLEGIHELQASDQQTLLSILKKELKAYSVFDLMKIQA
                     HLEKDFRDLPPEYKKRLQQKLSEHLFVNFKSILHNDNYQNSQLDMEQYREFLEWFKSR
                     LDDEMPGMTVLYYLSALYNIFITKTPPHPEGTPFPGGFFVEKVEDKYYCPVKDKQKDN
                     EEAICKFCIAKQMDMDTFNSNPMNY"
     gene            1825..2040
                     /locus_tag="VKL21_05510"
     tRNA            join(1825..1862,2005..2040)
                     /locus_tag="VKL21_05510"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1859..1861,aa:Trp,seq:cca)
     gene            complement(2043..3239)
                     /locus_tag="VKL21_05515"
     CDS             complement(2043..3239)
                     /locus_tag="VKL21_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HMB45260.1"
                     /translation="MNNEEVRALRKAFDSVKERQAQNLLESFDDRTKRLISSRDLCVG
                     NEGLLEHAIVNLRKNGMKVYLAREKQEALEIILKEIGKEKIIVKSKSNVTKELELTKF
                     LEKNGLTVVETDIGDRILQILDAKPSHPTGPVTHLSAKEIAKGISKYYDLPVTQTPED
                     IVKIVKADIVSNLEKAKIGITGANAITSEEGSILMTHNEGNIHEVMRKEKHIVVTSID
                     KIYPDIEAAINMMKILSFNATGSIIPSFVDIISGVSKTADVEKKFVKGVHNPSEIVLV
                     LVDNRRSDLARKGFKELLKCIGCGNCLLHCPMYNTIGNEYAIDSYLGGKGLAYYSLYT
                     NEPHEKLELCLRCGKCRENCPLELDIPEIIKNLRSTGVSSELYYFLKSHMVWVYYQSL
                     LRLDLK"
     gene            complement(3232..4500)
                     /locus_tag="VKL21_05520"
     CDS             complement(3232..4500)
                     /locus_tag="VKL21_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45261.1"
                     /translation="MVSINPNYPFRITGKNIRPGINKWFAIRRNQYLAIALALFASFI
                     IRIYLSQIEGYGYDIWTFKTWSRWVYYTGFAHFYNSIRSDYPPFYIYFLWAIGTFYKS
                     FLSFSFDIDSPVFTFLIKMPAMIADIFTAFLIFLIIRRYGSFRLAFLSMISYSFNPAI
                     IYDSAIWGQVDSVYTLFFMLALMLFVSDKPMHSGVCLALAILTKPQSLVLLPLFALFV
                     IRKHSIFTSAKVAAVSCAAFVVVALPFYLDTSIFDLFRLYSSSYIQYPYTSLNAFNIW
                     AFTGMFQPDETKFLLMTYRMWGYLLFGMIFIYVVYFTLKNKDDMSIYISSAILFFAFF
                     MFFTRMHERYLFPFFAPLAVAMTMDRRLCYVYVLATITFLFNLHFVLEETKSGFGLPN
                     GKLLIPVAAGINLILLIFTIYCYSKRKHHE"
     gene            4593..4880
                     /locus_tag="VKL21_05525"
     CDS             4593..4880
                     /locus_tag="VKL21_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HMB45262.1"
                     /translation="MLIEQINLEKGSALGFKMDMEYAPLLVIRAGCGFVMCGYLNMDI
                     ANKLGDVAVRVTGVKSFEDVLNAKASDVSEAAKKRGILAGITAKEALNMMF"
     gene            4990..5571
                     /locus_tag="VKL21_05530"
     CDS             4990..5571
                     /locus_tag="VKL21_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HMB45263.1"
                     /translation="MAVAKTEGWKAKQWYNLVAPDMFGKSNIGETIADEPEKLIGRNI
                     EVTLGELTNDLSKQNTKLILKIDRVGGDSAYTKYMGHQLTQDYLRSLVKRETSSVETN
                     VSVKTRDGYTIRVKPSCFTIKRARENQVKAIRQIMNTVIEAKAREMDMEQFVQDVVTG
                     KLSASIYHDVKPIYPLRRVEIRKTEIEAEPKAS"
     gene            complement(5596..>5911)
                     /locus_tag="VKL21_05535"
     CDS             complement(5596..>5911)
                     /locus_tag="VKL21_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HMB45264.1"
                     /translation="SYLMIRQAVEEPEKRARLSAVFGIIGFISVPISFLSVRFYSKMH
                     PCVVPPCPSGGGGGIGGEVLSYLLLNFAAYFLLAASLIMLRMDNERLKEKANELKRKN
                     NL"
BASE COUNT         1762 a         1228 c         1198 g         1723 t
ORIGIN      
        1 ggaaaggatt tgatgggcct gacgttggca tacaggttga acttctggcg gatcgacact
       61 gctacgctct ttggagagcc tgcaccgcca ggtgttgtgg tggggccctt aaaacctgca
      121 tcagtcttgc cgagaatgtc ccatgtttcc tggggtatca gagaatctcc tccattttct
      181 ttccaccatt gctctcctcc ttcgcacaat atgaactcaa cttttgtacc ggccgctttt
      241 gctaccctga gcatgctgtt tactagttca ggtcctacgc cgtcgccttt tatgactgct
      301 gtttttttgc tcatgtttcc cttcattcat ccgaaatgct attaagttta tccaggtaca
      361 ttttcaatga gatcaatgac agagttgtcg agattatatc tttccagctc ccgttttcct
      421 gctgttttcc gagcagccat ttaatggatg gctcgatatc ttcttccttt gtccctgcaa
      481 ggatcaaagc ctggataaca aggttgctcg ttgcgatata ggtccagccc gagtcttctc
      541 tttttgaaat cagccagctt gcccgttctt ttataaaatc gcgatattct tccctgtcct
      601 gcttgataag ggcagtgatt atcagcgatg ttgtccctgc atgctcccat tttggcccgt
      661 aattgcgaat cagccactcg catccgggta tgttctttat ctcgcgatca cccagggcca
      721 tgagggcata cgaagtgtcg atctcattgt tgttccagtt ttcaccgcgc tgctgttcct
      781 gtatccagcg tattatttca acgtgtacct tttcgcatcc caaaagcgcg atgcatgctc
      841 ttgccgtatc attgatgggt gtctgggttt cccagtatcc atcctttttc attgatatca
      901 atttctcgat gagaatcgat gcggattcat cccacaaaga tagcgcctgg atggagcggg
      961 agatatcctt gacggtttgt ggttcttgtt cttgtaacca ggccagtccg cgcgatgctg
     1021 cagcttgtat aacattaata tctgcttcca ttatataccc ggaatttgat ctgtgtgttt
     1081 gaagttccat tttttcataa tccataattt tgtaaatttt tgcaatttca tttctaaaaa
     1141 accaattgcc tattgaagtt atagtatcat attatcttat gcgtcctcat gatcaattga
     1201 tgttattccc gcgaatacca taattgttaa tatcacaccc cttcattcag atatttgtgg
     1261 ctcaatcagt attcaggaaa ttggaaggta tccatgaact ccaggccagt gaccagcaaa
     1321 cacttttatc tatcctaaaa aaagaactta aagcatatag tgtttttgac ctgatgaaaa
     1381 tacaggctca cctggagaaa gacttcaggg atctgccgcc tgaatataaa aaaagattac
     1441 agcaaaaatt atcagaacat ctgtttgtta atttcaaaag catccttcac aatgataact
     1501 accagaacag tcagctggat atggaacaat atcgggaatt tcttgaatgg ttcaaatcca
     1561 ggctggatga tgaaatgcca gggatgacgg tactatatta tctaagtgcc ctgtataata
     1621 tttttattac gaagacacca ccccatcctg aaggaacacc atttccaggg gggttctttg
     1681 tggaaaaagt tgaagataag tattattgcc ctgtaaagga caagcaaaaa gataatgagg
     1741 aggctatctg caagttctgt atagccaaac agatggatat ggataccttt aatagtaatc
     1801 ccatgaatta ttagcgattc ataaggggct gtggcctagt ccggcatggc gacgggctcc
     1861 agcggaaaac cggagtaatc cgggtgtgat gatgagtaaa tggtctaccg agtgaaccgg
     1921 acgcggtgag caatgaggaa ccgttggagc actgaacgtg ctgctcaaag attaaattgc
     1981 caataaactg gcattttcgg agagacccgt cgatcgtgag ttcaaatctc accagcccca
     2041 cattatttaa gatccaatct caaaagagac tggtaatata cccataccat gtgggatttc
     2101 aggaaataat acaactctga agatactcca gtacttctca gattcttgat tatctcagga
     2161 atgtcaagtt caagggggca gttttcacgg catttcccgc acctcaggca cagctccagt
     2221 ttctcatggg gttcatttgt atatagtgaa taataagcta atcccttccc ccccaggtaa
     2281 ctatctatcg cgtactcatt tccgatagta ttatacattg ggcaatgcag caaacaattc
     2341 ccgcatccta tgcatttgag aagttcctta aatcctttcc tggcaaggtc actccttcta
     2401 ttatcgacaa gcacaagaac tatttcagaa ggattatgaa cccccttaac gaatttcttt
     2461 tccacgtccg ctgtcttact gaccccactt attatatcta caaatgaagg gattatcgaa
     2521 cctgtagcat taaaagacag gatcttcatc atgttgatag cagcttctat atccggatat
     2581 atcttgtcaa ttgaggttac cacaatatgt ttttctttcc tcatcacctc gtggatattc
     2641 ccttcattat gggtcatgag aattgagcct tcctctgagg tgatggcatt agcccccgta
     2701 attccgattt ttgccttttc caggttggaa acgatatcag cttttactat tttcacgata
     2761 tcttcaggtg tttgcgtaac tggcagatca taatattttg aaatcccttt tgctatttct
     2821 tttgcggaaa gatgtgtcac agggccggtt ggatgtgaag gctttgcatc cagtatctgt
     2881 aatatcctgt cccctatatc agtttctaca acagtcaatc catttttttc gagaaatttt
     2941 gttaattcca gttccttggt aacattggat ttggatttta ctataatttt ttccttccct
     3001 atctctttca atattatttc aagagcttcc tgcttttccc tggcaagata gaccttcatt
     3061 ccgtttttcc tgagatttac tatcgcatgt tcaagaagtc cttcatttcc cacgcaaaga
     3121 tctcttgatg aaataagtct ctttgtccta tcgtcaaaac tttccagcag attttgagcc
     3181 tgcctttctt tcacggaatc aaatgcttta cgaagggctc ttacttcctc attattcatg
     3241 gtgtttcctt tttgaataac aataaatcgt aaatattaat aagataaggt taatccctgc
     3301 cgcgacaggt attaataatt taccgttggg taaaccgaaa ccactttttg tttcttcaag
     3361 cacaaaatgc aggttgaaca aaaaggtaat tgttgccaat acatacacat aacaaagacg
     3421 cctgtccatg gtcattgcaa ctgccagcgg agcgaaaaac ggaaaaagat acctttcatg
     3481 cattcgagta aaaaacatga aaaaagcgaa aaataatatt gcagaagata tatatattga
     3541 catatcatct ttgtttttca gggtaaagta cacgacataa atgaaaatca ttccgaacag
     3601 aagataaccc cacattcgat aggtcatgag taaaaatttg gtttcatcag gctggaacat
     3661 acctgtgaaa gcccagatat taaaagcatt taaagaagta tagggatatt ggatatatga
     3721 tgaagaatac agcctgaaaa ggtcaaagat tgacgtatcc agataaaaag gcaatgctac
     3781 aaccacaaat gcagcacatg atactgctgc tacttttgca gatgtgaata ttgaatgttt
     3841 tcttattacg aataaagcaa aaagaggcaa aagtacaagg ctctggggtt ttgtcaatat
     3901 ggccaatgcc aggcagacgc ctgaatgcat cggcttatcc gatacaaaaa gcatcagggc
     3961 gagcatgaaa aacagggtgt aaacagaatc cacctgtccc catattgcag agtcatatat
     4021 tattgccgga ttgaaagaat atgagatcat tgataggaaa gccaatctaa aactgccata
     4081 tcttcttatg atcaggaata tcaaaaaagc cgtgaaaatg tctgctatca tcgcaggcat
     4141 tttaattaag aaagtaaaaa caggggaatc aatgtcaaaa gaaaatgata gaaatgattt
     4201 ataaaatgtt cctattgccc acaggaaata gatataaaag ggaggataat cagacctgat
     4261 actattatag aaatgagcaa aaccggtata atagacccaa cgactccagg tcttaaaagt
     4321 ccagatatca tatccgtatc cttcaatttg tgaaagatat attcttatta tgaatgaagc
     4381 aaataacgca agagcaatgg ctaagtattg atttctcctg atggcaaacc atttattgat
     4441 gcctggtcta atgttttttc cggtaatcct gaaagggtaa ttgggattta tggataccat
     4501 cccctggcac ttagatgcga aaccaatatt tatagaaacg ggttcaaagg gacgaaataa
     4561 aatatttcta tcggtatata caatgtatga ctatgttgat cgaacagata aatcttgaaa
     4621 aagggagtgc gcttggattt aagatggata tggaatatgc cccgctgctt gtaattcgcg
     4681 ccggctgcgg ctttgtcatg tgcgggtatc tgaacatgga tattgccaac aagctggggg
     4741 atgtggccgt gagggttacc ggggtcaaga gctttgagga cgtgttgaat gcgaaggctt
     4801 cggatgtctc ggaagccgca aagaaacggg ggatactggc tggaataaca gccaaagaag
     4861 cgttgaacat gatgttttag caaaacaaaa aaaaatgtcc aaaagggtta agtatccatt
     4921 gctctattaa acgcatgatt ttgtgaactt atttcacatt ttaatcatat tatacctgga
     4981 ggaaattaat tggcagtagc aaaaacagaa ggctggaaag ctaaacaatg gtacaacctt
     5041 gttgcaccgg atatgttcgg aaaatcaaat atcggagaaa caattgctga tgaaccggaa
     5101 aagttgatag gaaggaacat cgaagtcaca ctcggggagt tgacaaatga tctctcaaaa
     5161 cagaatacca aacttatcct gaaaattgac cgcgtaggcg gcgattccgc ttatacaaaa
     5221 tacatgggcc atcaactgac acaggattac ctgcgctccc tcgttaagag agaaacatca
     5281 tccgtagaga caaatgtttc ggtaaaaacc agggacggct ataccataag ggttaaacca
     5341 tcgtgtttca ctataaaaag ggcacgtgag aaccaggtca aagctattcg ccagatcatg
     5401 aacactgtta ttgaggcaaa agccagggaa atggatatgg agcagttcgt acaggatgtt
     5461 gtaacaggaa aactgtctgc cagcatatac catgatgtaa aacccatcta tcctctccgc
     5521 agggtagaga tcaggaaaac agaaattgag gccgaaccca aagcctcata atctttcctt
     5581 ttttttttaa aatttttaga gattgttttt tcgcttcaat tcattggctt tttctttcaa
     5641 acgttcatta tccattctca gcattatgag tgacgcagcc agaaggaaat atgctgcaaa
     5701 attaagaagg agataagata agacttctcc tcctatacct ccaccgccgc cgctgggaca
     5761 tggcgggaca acgcacggat gcatctttga atagaaccgt accgaaagaa aacttatagg
     5821 gacgcttata aaaccgatta taccgaaaac cgccgaaaga cgggcccgtt tttcgggttc
     5881 ttccactgcc tggcggatca tcaggtatga c
//
LOCUS       DATGBN010000230         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_95663, whole genome shotgun sequence.
ACCESSION   DATGBN010000230 DATGBN010000000
VERSION     DATGBN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95663"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VKL21_05540"
     CDS             complement(<1..177)
                     /locus_tag="VKL21_05540"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMB45265.1"
                     /translation="MTIIKQKITPNLWFDRQAEEAAKSYTSIFKNSRVGRITRASKAG
                     FEIHGLPEGTVMTIE"
     gene            complement(199..336)
                     /locus_tag="VKL21_05545"
     CDS             complement(199..336)
                     /locus_tag="VKL21_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45266.1"
                     /translation="MRKRFYVIIATYEPVGKLKTGSFALDNPIKAEIERRKRLADESK
                     D"
     gene            complement(590..751)
                     /locus_tag="VKL21_05550"
     CDS             complement(590..751)
                     /locus_tag="VKL21_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMB45267.1"
                     /translation="MFAKQIQNGLDLTDEAKNITAIYDTSLEPGQGIPLHFHPDIEEI
                     YYILSRYAA"
     gene            complement(854..1471)
                     /locus_tag="VKL21_05555"
     CDS             complement(854..1471)
                     /locus_tag="VKL21_05555"
                     /inference="COORDINATES: protein motif:HMM:NF021418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2119 family protein"
                     /protein_id="HMB45268.1"
                     /translation="MFFKVLGSSNPTRLFVAGIHGDEETITRPIFEMMIKDIKITSGR
                     LIVVCLSRDCPYISTLNEAYYDSTNGKKLLELIREYRPGIYVELHTYKKDNHSNLIDP
                     DRKRKIGVPAFTELEEKVLIGSVSPFIRTSEFRREDFCFTLEMPDPYSEKALQIALHI
                     LRSISLSSSRDEILEKLRVQYPLQIQEAEKNFREFFKDMRNIFFF"
     gene            complement(1473..2507)
                     /locus_tag="VKL21_05560"
     CDS             complement(1473..2507)
                     /locus_tag="VKL21_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HMB45269.1"
                     /translation="MFNTASSNECIIKVSKNGPYIVSGKIPIFEYAINCDSKGIPINW
                     ILGKEYPVREKYELCRCGQSKNKPFCDGTHADINFNGTETADLEHSQAKEINGPELIL
                     KDVEKLCASARFCHRSGEIWEHIPKSDNPEIKKIAIRNSCDCPSGRLLLRDKKTGKSI
                     EPELGRSIALVADPFMGVNGPIWVRGAIPIQSSDGKIYERRNRVTLCRCGKSLNKPFC
                     DSSHYPEQSKEEITMEKSKSELKDIKGDLEDILKTKKRAFVLFYASWCSFCRTFLPIY
                     DRYFEKQPETFLRVMIDDREELMDRYSVEIVPTVIFFDNGRIKKRCDGEAGVGLSEKR
                     LIDIIKSSQE"
     gene            2748..4013
                     /locus_tag="VKL21_05565"
     CDS             2748..4013
                     /locus_tag="VKL21_05565"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMB45270.1"
                     /translation="MFTIITGAQFGDEGKGKIVDMMASKYDIVARFQGGDNAGHTVVA
                     EGKTYKLHLIPSGVLFDARLLIGPGTVMNPGILMEELAMLSGNGVEVPPSKLGIDAKT
                     SIIMPYHIALDGLRESKRAVKIGTTNRGIGFAYVDKVGREEIQMADIADKKRFMRKIE
                     EIAPQKEAAIKELGGDPGIARSEIEAYLEIGKKLRPYITDVSKEINEALKEGKTVLAE
                     GAQGTHLDIIHGTQKFVTSSSTIAGSACAALGVDPTKVNEVIGIIKAYITRVGEGPLP
                     TEQKNQVGEHLREKGGEFGTTTGRPRRCGWFDLLLARKSINLNGYTSMALTKLDVLSG
                     LDPLKLCIEYEMEGNIMKYMPELSDDVARCKPVYRDIPGWHEDISDTGSFVELPENAR
                     LYVELIEKLMDVEINYISVGPGRKQTFMK"
     gene            4039..>5203
                     /locus_tag="VKL21_05570"
     CDS             4039..>5203
                     /locus_tag="VKL21_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB45271.1"
                     /translation="MEKSIRLKVTEADQRDVGKGIVRIDEKFRNMIGVNIFDVVEIRV
                     EQHSTSAVVGRAYPQDEGLDVIRMDGLIRTNARTSLGEFVDVFKAEWKEAKKVTLAPV
                     IKNIHIYAPSESLRAIFHNRTVSKGDIISTTSVRRPRESFGTDVMFEQVFPEFFGGSF
                     GLGEIKLHVFSTSPGGIVKITDVTEVELLPEAVELPAELPEVMYEDLGGIKPALSKIR
                     EIIELPLKHPELFNRLGIDAPKGVLLHGPPGTGKTMLAKAVANESEAYFITINGPEIM
                     SKYYGESEHKLREAFDVAEKNAPAIIFIDEIDSIAPKRAEVTGEVERRVVAQLLSLMD
                     GLKSRKNVIVIGATNRPEALDMALRRPGRFDREIELRAPDREGRREIFQIHTRG"
BASE COUNT         1491 a         1061 c         1167 g         1484 t
ORIGIN      
        1 ctcaatggtc attaccgtgc cctccggcag gccgtgaatt tcaaaacctg ctttgctggc
       61 acgagttatc ctgccgaccc tggaattttt gaagatggaa gtataagatt ttgccgcctc
      121 ttcagcctgg cggtcaaacc atagattcgg tgtgatcttt tgtttgatga ttgtcataat
      181 tttattccta taagttctct aatctttgct ctcatcggca agtcgtttgc gtctttcaat
      241 ttccgcttta atcggattat caagcgcaaa cgatccggtc ttgagttttc cgacgggttc
      301 ataggtggcg atgatgacgt agaagcgttt tctcatcgcg ttcggtggtg gtgaccagaa
      361 catactcacc catctcttta ccaggctgtt cagtctgcta gccgaacaca gtcttataaa
      421 actttctcat acggacctta tctttcgccg acatttcaaa atgtacgaca tgattcattt
      481 tcatcagatt cccactaata aaataattat gtgttcgatg atataaggat ttttcgcagt
      541 ttcatagatt tattcattct gctgggcttg cgcattgacc ggtgtactct caagctgcgt
      601 atctggacag gatataataa atttcctcga tatcaggatg aaaatgcaac ggaataccct
      661 gccctggctc aagtgaggta tcatatatcg ctgtaatgtt ttttgcctca tcagtcagat
      721 caagtccgtt ctgtatctgt tttgcgaaca tagaattaca attaaaatga gtgaaataaa
      781 taacttttgt cctgtcaagg ctaaaacttc aatttatacc gtaatttgtt gcttattgac
      841 acttggcaag acactagaag aagaatatat ttctcatatc cttgaagaat tcacgaaaat
      901 tcttctcagc ttcctggatt tgcaggggat attgtacccg gagtttttca agtatctcat
      961 ctctgctgga agataacgat attgacctca ggatatgaag cgcaatctgt aacgcctttt
     1021 cagaataagg atctggcatt tcaagggtaa agcaaaaatc ttctctcctg aattcggaag
     1081 ttcgtatgaa aggtgaaaca gagccaatga gaactttttc ttcaagctcg gtaaaagccg
     1141 ggacaccaat ctttcttttc ctgtcggggt cgattaaatt cgaatgatta tcctttttat
     1201 aggtatgcag ctcaacatag attcctggtc tatattcccg aatgagttcc agaagtttct
     1261 tgccatttgt cgaatcataa taggcttcat tgagggtgct gatataggga caatctctgg
     1321 agagacatac cacgataagc ctgccacttg tgatcttgat atctttgatc atcatttcaa
     1381 aaataggtct ggttatcgtt tcttcatcac catgaatacc tgccacgaaa agtctggtgg
     1441 gatttgagct gcctaatact ttgaaaaaca tttcattcct ggcttgattt gataatatct
     1501 atcaaacgtt tttcgctcaa cccaacgcca gcttctccat cgcatctttt ttttattctg
     1561 ccattatcaa aaaaaatcac tgtaggtact atctccaccg aatatcggtc cattaactcc
     1621 tctctgtcat ctatcatgac gcgcaggaaa gtctctggct gtttctcaaa gtatctatca
     1681 tatattggaa gaaatgtccg gcagaatgaa caccaggacg catagaaaag gacaaatgcc
     1741 cgtttcttag ttttcagaat atcctccagg tctcctttga tgtcttttaa ctcacttttg
     1801 cttttttcca tcgttatttc ctctttagat tgttccgggt agtgactgct gtcacagaac
     1861 ggtttattga gagacttacc acatcgacag agggtgacgc gatttcttct ctcataaatc
     1921 ttaccatcgg aagattgaat tggaatcgcc cctctcaccc agattggacc atttaccccc
     1981 ataaaagggt ctgcaaccaa tgcgattgaa cgtccaagtt cgggctcaat actcttccct
     2041 gttttcttat cccggagtaa aagacggcct gaaggacaat cgcatgaatt cctgatggca
     2101 atttttttta tttctggatt gtcggatttg ggaatatgtt cccagatttc tcctgaccta
     2161 tgacagaaac gggcagatgc acagagtttt tcaacatctt tcaatatcaa ttctggaccg
     2221 tttatctctt tagcctgact gtgttcaaga tccgctgtct ctgttccatt gaaatttata
     2281 tcggcatggg tcccatcgca gaagggtttg ttctttgatt ggccacaacg gcacaattca
     2341 tatttttccc taactggata ttcctttccc aatatccaat taattggaat gcctttgctg
     2401 tcgcaattta tcgcatattc gaatattgga attttcccgg aaactatgta aggcccattt
     2461 ttgctaacct tgataatgca ttcatttgaa cttgccgtat taaacaccaa ttcgtttatt
     2521 caattaaaca ttttgaattt tgcgtttaca aacagtatct ttgaagataa aaatgttttt
     2581 caataaggat ggaaattggc agatcgattt catctttaat atattcttga tatcatagca
     2641 aataaatcct cataaagatt aaacccccgc tccacacgtt ttacatatca attatatttt
     2701 tttcaggtaa acttatcata tttcccgcca ggatagagaa ggtaaatatg ttcacaataa
     2761 tcacaggcgc acagttcggg gacgaaggta aaggaaagat agttgatatg atggcttcaa
     2821 aatatgatat tgtcgcacgg ttccagggcg gcgataatgc ggggcatacc gttgtagctg
     2881 aaggtaaaac gtataaactg catcttattc catcgggtgt gctttttgat gcgcgccttc
     2941 ttatagggcc tggcactgtg atgaatccag ggatattgat ggaagagctc gccatgcttt
     3001 ccggaaatgg cgtggaagtg ccgccatcaa aacttggcat cgatgcaaag acaagtatta
     3061 taatgccata tcatatcgct cttgacggcc ttcgtgaatc aaagcgcgct gtgaaaattg
     3121 ggaccacgaa cagggggatc ggttttgctt atgtggataa agtcgggcgg gaagagatac
     3181 agatggcgga tattgctgat aagaaacgtt tcatgaggaa aatcgaagaa atagcccccc
     3241 agaaagaagc agcgataaaa gaattgggcg gagaccccgg gattgcacgg tctgaaattg
     3301 aagcctatct tgaaatcggc aagaaattaa gaccttatat caccgatgtt tcaaaagaga
     3361 taaatgaggc attaaaggaa ggtaaaacag tcctcgccga aggcgcgcag ggtacgcatc
     3421 tggatataat ccacgggaca caaaaattcg ttacatcttc aagcaccata gcaggctcgg
     3481 cgtgtgcggc gctgggagtg gacccaacaa aagtgaatga agtgatcgga ataataaagg
     3541 cctatatcac acgcgtaggg gaagggccgc tgcccaccga acagaaaaac caggtaggtg
     3601 agcatcttcg ggaaaaaggc ggcgaatttg gaactactac gggaagacca agaagatgcg
     3661 gctggtttga cctgctcctg gcgcgaaaat ccattaacct gaacggatat acatccatgg
     3721 ctcttaccaa actcgatgtg ctttcagggc ttgatccatt gaagttgtgc atcgaatatg
     3781 aaatggaagg aaatataatg aaatatatgc ccgaactttc cgatgatgtt gcaagatgca
     3841 aaccggtata tagggatatt ccgggatggc atgaagatat ttcggatacc gggagtttcg
     3901 ttgagcttcc tgaaaatgcc aggctgtatg ttgaattgat tgaaaaattg atggatgtgg
     3961 aaattaatta catttccgtg gggccgggca gaaagcaaac ttttatgaaa taaacaacag
     4021 atattaatac ggggtaacat ggaaaaatca attagattga aagtaaccga ggcagaccag
     4081 agggatgtgg gaaagggaat cgtccggata gatgaaaaat tccggaacat gataggcgtc
     4141 aatatctttg atgtggttga gataagagta gagcagcatt ccacatctgc agtggttgga
     4201 agggcatacc ctcaggatga aggacttgat gtcataagaa tggatgggct tatacggaca
     4261 aatgcaagga ctagcctggg agaatttgtt gatgttttta aggcagagtg gaaagaagca
     4321 aaaaaagtaa cacttgctcc tgttataaag aatatccaca tttatgcgcc aagtgaaagc
     4381 ctgcgtgcaa tttttcataa caggacggtc tcaaaaggag atatcatatc cacgaccagc
     4441 gtgaggcgac cgagagaatc attcgggacg gatgtcatgt tcgagcaggt attcccggaa
     4501 ttcttcgggg gttcttttgg ccttggcgag ataaaattgc atgttttttc aacatcaccg
     4561 ggtgggatag ttaagataac cgatgtcact gaggtagaac tccttcctga ggctgttgag
     4621 cttcctgctg aactacccga ggtaatgtat gaagacctgg gtggaataaa acctgccctg
     4681 agtaagatcc gtgaaatcat tgaattgcct cttaaacatc cggaactatt caacaggctt
     4741 ggtatcgatg cgccaaaagg agtgcttctt cacgggccgc cgggaacagg aaaaaccatg
     4801 cttgctaagg ccgtagcgaa cgaaagcgag gcgtatttta tcactataaa tggccctgaa
     4861 ataatgtcaa aatattacgg ggaaagtgaa cataaacttc gtgaggcatt tgatgtggcc
     4921 gagaaaaatg ctcctgcgat catctttatt gatgaaatag acagcattgc tccaaaacgc
     4981 gctgaggtga caggtgaagt agagcgccgt gtcgtggccc agcttctttc acttatggat
     5041 ggcctgaaat caagaaaaaa cgtgatagtc attggcgcta cgaaccgtcc agaagctctt
     5101 gatatggcgc ttcgaaggcc gggcaggttt gaccgtgaga tcgagcttcg agcgccagac
     5161 cgtgaaggga ggcgtgagat attccagata cacacccgcg gta
//
LOCUS       DATGBN010000231         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_134851, whole genome shotgun sequence.
ACCESSION   DATGBN010000231 DATGBN010000000
VERSION     DATGBN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134851"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1230
                     /gene="tdh"
                     /locus_tag="VKL21_05575"
     CDS             205..1230
                     /gene="tdh"
                     /locus_tag="VKL21_05575"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HMB45272.1"
                     /translation="MKAVRKTKEGPGLELVDIPVPVPEDHEVLVKVRACSMCGTDVHI
                     YNWEHPWSQGRITPPRTIGHEVCGEVIKIGDDVTLFREGDVVSAESHIFDGKCMQCRR
                     GNMHICENLKFFGVDVDGFFAEYAVIPETNAWKNPPDMPIEIATLQESIGNSVYTVFS
                     NNVTAKSVAVFGGGPTALFAIGLLKAAGATCIISVAGSEQHLEIARKMGADIVINRHE
                     KDPVKEIMDKTGGTDVFLEMSGSANAINQGLKALRPGGRASILGLPTGDVSIDMSRDF
                     VMKDITVRGVYGRKIWDTWITTSRLLSTGKFDPTPVITHRIRLDEFEQGFEAMKEGKA
                     GKVVMFP"
     gene            complement(1264..1926)
                     /locus_tag="VKL21_05580"
     CDS             complement(1264..1926)
                     /locus_tag="VKL21_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMB45273.1"
                     /translation="MAKKKQKIPEKKSGLKTIHFILALLVILALAIYFLNAGEKSPEK
                     TIAGDYSKLNNPSTSEPGKVKIMEFLNFKCSHCYDLQKSMPQLLQKYGDKVEITYIPI
                     NFTGQSTKSVEAYIIAKQMGKGDEMYEALFTAEFEKNMDIMESTIAIETVASGIGLGP
                     DFNRKFESGEAAKEALDNFKLANKYRIEGTPTVIINGNLMVVPTIANLDKVIGSILEQ
                     GS"
     gene            complement(1988..>2184)
                     /locus_tag="VKL21_05585"
     CDS             complement(1988..>2184)
                     /locus_tag="VKL21_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45274.1"
                     /translation="LMHASGVIGTLSAPLIVALIALDKGASGIAQNLFYLFLLLIVFE
                     LLTSTKIGDLMKAKREYGYR"
BASE COUNT          632 a          428 c          490 g          634 t
ORIGIN      
        1 aataataata cctatcccga aaaaggaatt gttcagatac catttaaagg ataggcatag
       61 tgatatatag tatcaaagac atatatagat tgttcagcta acctgagaaa gtcatttcaa
      121 tatagaatat atcccactaa aaaacaagaa gctccgtacc tttaggtcgg agtcgttcac
      181 taatatagtt ctcaggggga aatcatgaaa gccgtcagga aaacaaaaga aggaccggga
      241 cttgaacttg tggatatacc tgttcctgta cccgaagatc acgaagtgct tgtgaaagta
      301 agagcatgtt ccatgtgcgg taccgacgtc cacatttaca attgggagca tccctggtcg
      361 caggggagga tcacgcctcc ccggaccatt ggccacgaag tttgcggcga ggtgatcaag
      421 atcggagatg atgtcacact tttcagggaa ggggacgtgg tatcggccga atcgcacatt
      481 tttgatggca aatgcatgca atgcaggaga gggaatatgc atatttgcga aaatctcaag
      541 ttcttcggag tggatgttga tggttttttt gcagagtatg cagtaattcc tgaaaccaat
      601 gcatggaaga atcctccgga tatgccaatt gagatagcga ctctccagga atctatcggg
      661 aacagcgtgt acactgtatt cagtaataat gtaactgcaa agtcagtcgc agtgttcgga
      721 ggcggcccga ctgcgctatt tgccattggt cttcttaaag ccgctggggc tacatgtatt
      781 atttctgtgg cgggctcgga acagcacctt gaaatcgcaa gaaagatggg tgctgatatt
      841 gttatcaaca ggcatgagaa agaccctgtg aaggagataa tggataagac cggaggaact
      901 gatgttttcc ttgaaatgtc aggaagcgca aatgcaataa atcagggttt aaaagcattg
      961 cggcccgggg gcagggcatc catactggga ttgccaactg gagatgtgag cattgatatg
     1021 tccagggatt ttgtcatgaa ggacataaca gtgcgcggag tttatggaag gaagatatgg
     1081 gatacatgga tcaccacatc aaggcttcta tcaacgggta aattcgatcc cactcctgtg
     1141 ataactcaca ggataagact ggatgaattc gagcaggggt ttgaggctat gaaagaaggt
     1201 aaggctggaa aggtcgtgat gtttccttga gatgaagcat gaataaccca ccttgagtcg
     1261 tggttaagaa ccttgttcca gaatcgaccc gataacctta tcaagatttg caattgtggg
     1321 cacgaccata aggttcccat tgataataac agtaggagtc ccctcgattc tatatttatt
     1381 cgccagtttg aaattatcta aagcttcttt tgctgcttca ccgctttcaa atttccggtt
     1441 aaaatccggt ccaagcccta tgcctgacgc aacagtttca atcgcaattg tgctttccat
     1501 tatatccata ttcttttcga actcggccgt gaataatgct tcatacattt catctccttt
     1561 tcccatctgt tttgcaataa tatatgcttc aacgctcttt gtggattgtc cggtaaaatt
     1621 gatgggaatg tatgtgattt caactttgtc accatatttt tgcaaaagct ggggcatgct
     1681 tttttgaaga tcataacaat gactgcattt gaaattcaag aattccatga tcttaacttt
     1741 acctggttct gatgtggagg gattattcaa tttggaataa tcgcccgcta ttgttttttc
     1801 aggactcttt tccccggcat ttaaaaaata gatcgccagg gctaaaatta caagcagtgc
     1861 cagtataaaa tgaattgttt taagacctga ttttttttca ggtatttttt gtttcttttt
     1921 tgccattaaa ttggtttaaa tatggttttc tcttataaat attgcgtttt gagattatgc
     1981 ctgattttca cctgtatcca tactccctct ttgctttcat caaatcccct atctttgtag
     2041 aagtcagaag ttcaaaaact ataagcaata aaaacaggta aaaaagattt tgtgcgatcc
     2101 ccgaagctcc cttatccagc gctattaatg ccactatcaa aggagccgaa agtgtcccga
     2161 taacacctga agcgtgcatg agcg
//
LOCUS       DATGBN010000232         4927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_483673, whole genome shotgun sequence.
ACCESSION   DATGBN010000232 DATGBN010000000
VERSION     DATGBN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483673"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VKL21_05590"
     CDS             complement(<1..416)
                     /locus_tag="VKL21_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45275.1"
                     /translation="MTAELKQNAATGALASHFKPYPVYKDLGVEWLGKVPEHWEIWKI
                     KHSTYVKGRIGWKGLTSNDFLDQGAYLVTGTDFADGKVNWGTCYHVSYERFDEDPYIQ
                     LKEKDILITKDGTIGKIAIAENLPDKATLNSGIFLI"
     gene            957..1865
                     /locus_tag="VKL21_05595"
     CDS             957..1865
                     /locus_tag="VKL21_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45276.1"
                     /translation="MIIFLIFSSGIFTTQEKKEKPKPSIPSISIGCNRVLWENAQEVL
                     EATVNNISKPSFEWKMNGTPISTNQKLLQKFGIGEHLISLNVTFNNRTLSAKKSIQVI
                     DSVDGITMSQYPISKNQWGFQTMFKGKNTGVKEVMVYVDSLPPLQVNDCGAASTKALF
                     SGKHKWRAQYRDVNIASGTFDVKETSELKIDRIEIAPSYTAGSIVKAKLILVNTGSMA
                     ITEFTTNTLALNNDYAWMGDRAKREYSDQYNADIKPGETYEIPIQMTIPEEVSGIRPS
                     GKYTITVQVLLKGVKVDTKIVNTVVK"
     gene            complement(1923..2138)
                     /locus_tag="VKL21_05600"
     CDS             complement(1923..2138)
                     /locus_tag="VKL21_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45277.1"
                     /translation="MECQICEESTSNEKYCDRCGRIMEKVIREVGPDIWEKIDDCSYI
                     YPMVKRVAEGDLRTQDVVNEILKGEAD"
     gene            2289..3638
                     /locus_tag="VKL21_05605"
     CDS             2289..3638
                     /locus_tag="VKL21_05605"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HMB45278.1"
                     /translation="MYNDIITKIKRAKSASIPLASVKGVIKNSALAAMAHALDSNRKK
                     IISSNKKDVTAAEKLAEAGKLSRALVKRLKVDDTKIDEMIAGVRDVLKFEDPVGKTLS
                     ALELDSGLELYQVSCPIGLIGVIFESRPDVVPQIMSLCLKSGNATIFKGGSEAGNSNR
                     AIFDVLVNAIENTQGMPHGAFALMETREEVNEMLKLDEYINLIIPRGSNEFVKYIQDN
                     TRIPVLGHSSGICHVYVDKEADLSKAYDVCYDSKVQYAAVCNAMETLLVHRDIAGTFL
                     PEMAKKYDASGVELRCDEQSFDLLKEMGFLKAIMHATREDWKTEYNDLILSIKIVDSM
                     EEAIEHINKYGSHHTDAIITKNKTSASKFIDLVDSSSVMWNASTRFADGFRYGKGAEV
                     GISTNKIHARGPVGMEGLLIYKYALLGNGHKVSDYVGKDARKYTHKKLNAEFSEKMG"
     gene            3648..4781
                     /gene="proB"
                     /locus_tag="VKL21_05610"
     CDS             3648..4781
                     /gene="proB"
                     /locus_tag="VKL21_05610"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HMB45279.1"
                     /translation="MDPNVDRKELFSGLKKIVIKIGTSSLSNEDGSFNRNLTEDIASQ
                     VAHLRKIGITVIIVSSGAIGIGVIELKMPSHPREIPLRQAAAAVGQNILMQEWMTAFN
                     KHDLKVAQIFLTYEAFSNRMTYLNLRNSISALLDAGVVPIINENDPICVHEIEATFGD
                     NDKLSAMVASKVEAELLILLSDIDGLYNKNPKKNEDARLIGIVEKITPEIESYGGSPT
                     SAKGVGGMRTKIDAAKITSIAGCHMVIVNSAIDEVVIKVINGENIGTLFLARNGKFKN
                     RTRWIILSKTSGKLIVDKGAKDAIKKSMGLLPSGIIEVAGMFDRGDIVDIENEGEMFA
                     RGITDYTSSELARIKGKHSDMIEKILGYKNYDEAVRKSNIGLL"
BASE COUNT         1587 a         1007 c         1038 g         1295 t
ORIGIN      
        1 cttattaaaa aaatgccact gttcaaagtg gctttatctg gcagattttc agctatagca
       61 attttaccaa ttgttccgtc ttttgtgatc agtatatctt tctctttcaa ttggatatat
      121 ggatcttcat caaatcgttc gtaacttaca tgataacagg ttccccaatt tactttacca
      181 tcagcaaaat cagttccagt aaccaaataa gcaccttggt ccaagaaatc attggaagtc
      241 aaacctttcc aaccgattct accttttaca taagttgaat gttttatttt ccagatttcc
      301 caatgctctg gcaccttccc caaccattcc actcccaaat ccttatacac aggataaggc
      361 ttaaaatggc tggcgagcgc gcctgtggct gcattctgtt tgagttctgc ggtcattgcg
      421 cggcctcttg ttctgttgtt gaagaaaaat gccacttata tctgattttt tcgtctgcat
      481 tataaacgat agaacgcgga ttgcgcagat gacgcggatg gcgcgtggat tggattatcc
      541 gtgcaaatcc gcgtaatcag cgtcatccgc gttctattta gactaaattg aagatttaat
      601 agaaatcact gattttcttt tttcccatat tgaaatattt gttttaagtt tttctttatc
      661 tcctattcta tttattatat ctatgttagc atcattatca aaatactacc caactccagc
      721 catagaatgt tagaaccatc atcaagtgtg gcatcagccc attctacatc atttttccaa
      781 tagaagctac attcatcgtc tacagaattc gcatatacaa cataaatatt aataagacat
      841 atactaaaat tagttcaatc agagcataat tctaccataa ggagaaatat aatattttca
      901 aaataactaa gcaaaaacta aaaaagaaat atttcttatt gatactggga gcaataatga
      961 ttatttttct aatcttcagt tccggtattt tcacaaccca ggaaaagaaa gaaaaaccaa
     1021 agccttccat accttccata tccattggct gtaacagggt cttatgggaa aacgcacagg
     1081 aagtcctgga agcaacagta aataacataa gtaagccgtc atttgaatgg aaaatgaatg
     1141 gcaccccaat cagcacgaac cagaagctct tacaaaaatt tgggatcgga gagcatttga
     1201 tatcgctcaa tgtcactttc aataaccgga cgctttctgc aaaaaaatca atccaggtca
     1261 ttgattcagt cgatggcata acgatgagcc aatatccaat ttcaaaaaac cagtggggat
     1321 tccagaccat gttcaaggga aagaatacag gcgtaaaaga agtgatggtc tatgttgatt
     1381 cattaccccc tttgcaggta aatgactgcg gtgcagcttc aactaaagcc cttttttcgg
     1441 gaaaacacaa atggagggcc caatatcgtg atgtaaacat tgcatccggc actttcgatg
     1501 tcaaggaaac aagcgaattg aaaatcgaca ggatcgaaat cgcgccaagt tatacggcgg
     1561 gaagcattgt taaggcaaaa ctcattcttg taaatacagg gtcaatggcg atcacagaat
     1621 tcacgacaaa tacccttgcg ctaaataatg attatgcatg gatgggagac agggcgaaaa
     1681 gagaatattc agatcaatat aatgcggata taaaaccggg tgagacctat gaaatcccga
     1741 tacagatgac gatccctgaa gaagttagcg ggataagacc ttccggaaaa tatactataa
     1801 cagtacaagt attgttaaaa ggtgtaaaag tagatacaaa aattgtaaac acggtagtaa
     1861 agtgaaacta aaaaataata gaaatccccc ttcttcgcgc cgggaaatat atctgttctt
     1921 tttcagtcgg cttcaccctt gaggatttcg ttcaccacat cctgtgtcct gaggtcacct
     1981 tcagcaaccc ttttcaccat cggataaata tagctgcaat catcgatctt ctcccagata
     2041 tcgggcccaa cctctctgat tactttttcc ataatccttc cgcatctgtc gcaatatttc
     2101 tcatttgagg ttgattcttc gcaaatctga cattccatat ttttcactcc ctgccaaata
     2161 gattcatgct tataagataa atagatattg cagggttttc atatattaca ttgctgcaaa
     2221 aaagaccata tctataaaaa ctaaaatgat gtaaggaatc ccaattaaaa tacaggtttt
     2281 tatagatcat gtacaatgac atcataacaa aaattaaacg tgcaaagagc gcctccatcc
     2341 ccctggcaag cgtaaagggc gtaattaaga acagcgcgct tgccgcaatg gcgcatgcgc
     2401 ttgattcgaa ccggaagaaa ataatctctt caaataaaaa agacgtcaca gcagcagaaa
     2461 agcttgctga ggcaggaaag ctctcaagag ctcttgtcaa gcggcttaaa gttgatgata
     2521 caaaaataga cgagatgata gcgggagtga gggatgtcct gaaatttgaa gacccggtcg
     2581 ggaaaacact ttcggccctg gagctggaca gcggtcttga attgtatcag gtaagctgtc
     2641 ccatcggatt gatcggggtt atttttgaat cccgtccaga tgttgttcca cagataatgt
     2701 cactttgcct gaagagcgga aatgccacca ttttcaaggg cggcagcgag gcaggtaatt
     2761 ccaacagggc aatatttgat gtacttgtaa atgcaatcga aaatacgcag ggaatgccgc
     2821 atggtgcatt tgcacttatg gaaacaaggg aagaagtaaa cgagatgcta aaacttgatg
     2881 aatacatcaa cctcataatc ccgcgcggct caaacgagtt cgtaaaatac atccaggata
     2941 acacgcgtat cccggtgctt ggccattcat caggcatctg tcatgtgtat gtggacaaag
     3001 aagctgacct ctctaaagca tatgatgttt gttatgattc caaggtccag tatgctgcag
     3061 tctgcaatgc aatggaaaca ctacttgttc acagggatat tgcaggaaca tttcttccgg
     3121 aaatggcaaa aaaatacgat gcatcaggcg tagaactcag gtgcgatgaa caatcattcg
     3181 atctgctaaa agaaatggga ttcttaaaag cgataatgca tgcaacccgg gaggattgga
     3241 aaacagaata taatgacctt atcctttcca tcaaaatagt agattcaatg gaagaagcga
     3301 tcgaacatat caacaaatac ggttcccatc atacggatgc catcatcacc aaaaacaaaa
     3361 ccagtgccag taaattcatt gatctagtgg attcctccag cgtgatgtgg aatgcgtcta
     3421 cccgctttgc ggacggattc cgttatggaa aaggagctga ggttgggatc agcactaaca
     3481 aaatacatgc ccgcgggcct gtaggaatgg aaggtctgtt gatttacaag tatgcgcttc
     3541 tgggtaatgg tcataaggta tctgattatg tgggaaagga cgccaggaaa tatactcaca
     3601 agaaattaaa tgcagaattt tctgaaaaaa tgggataagg agttttgatg gatccaaatg
     3661 ttgaccgcaa ggagcttttt tccggcctga aaaaaattgt cataaaaata ggtacatctt
     3721 ccttatccaa cgaagacgga agctttaacc gcaatttgac agaagacata gccagccagg
     3781 tggcgcatct tcgcaaaatc gggataaccg taataatcgt aagctccggc gccatcggta
     3841 taggcgtcat cgagcttaag atgccatcgc atccacgtga aattccactt cgccaggctg
     3901 cagcggctgt tggtcagaat atccttatgc aggaatggat gaccgcgttc aataagcatg
     3961 acctcaaagt tgcacagata tttctgacat acgaagcctt ctccaacagg atgacatatc
     4021 ttaacctgcg aaacagcatc tcggcgctcc ttgatgccgg cgttgttcct atcatcaatg
     4081 aaaacgaccc catttgcgtg catgagatag aagcgacctt cggtgataat gataaacttt
     4141 cggcgatggt cgcaagcaaa gttgaggccg aacttcttat actcttgtcg gatatcgatg
     4201 gattatacaa taagaatcca aaaaagaatg aagacgccag gctcataggt atcgtggaaa
     4261 aaatcacgcc tgagattgaa agctatggag ggagcccaac aagcgccaaa ggtgtgggtg
     4321 ggatgaggac aaaaatagat gcggccaaaa ttacctctat cgcaggatgc cacatggtaa
     4381 tcgttaacag cgccatcgat gaggttgtca taaaagtcat aaatggggag aatatcggaa
     4441 cgcttttcct ggcacgcaat gggaaattca agaacaggac acgctggata atcctctcaa
     4501 agacttcagg taagctcatc gtggataaag gcgcaaagga tgccattaaa aagagtatgg
     4561 ggcttttgcc ctcagggatc attgaggtcg caggcatgtt tgaccgcggg gatattgtcg
     4621 atatagagaa cgagggggag atgtttgcca gagggataac ggattataca tcttccgagc
     4681 ttgccaggat caagggcaaa cattctgata tgatcgaaaa gatactcgga tacaagaatt
     4741 atgatgaggc tgtaaggaag agtaatatcg ggttactgtg attataaagg aaacaatggc
     4801 aaatcctgta agaattatcg gacagcctct ttagcgtgag gtagttcact tccgactgcg
     4861 ccttaaaatg aaaagatatg aaagtattat agatatcaat ccatagatta ttcccggttc
     4921 ggcaagt
//
LOCUS       DATGBN010000233         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_328804, whole genome shotgun sequence.
ACCESSION   DATGBN010000233 DATGBN010000000
VERSION     DATGBN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328804"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1006)
                     /locus_tag="VKL21_05615"
     CDS             complement(125..1006)
                     /locus_tag="VKL21_05615"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HMB45280.1"
                     /translation="MNASENSCVDCHKTLTPFIEEQKRFNQIRIQHLERNAACSLECH
                     EDRVRQLATANFQQWSESVHALKGVTCDACHGGDPKQATKEKAHINFKNITDPESPVY
                     YTNVPKTCGKCHSKELDNFENSTHYKKLESLQLAPTCTTCHIPHTFTVMNPEDFRNSC
                     GNCHSVYKKVAPYDIPLKAEDMLTKVNKLKFNIDNARQDIFWAQKNGTDVGQAGQYTD
                     NALAILGNLAPMWHEFNLTHFEDQINAANLEVKKAEDIVKPAPVPTETAAAGAPGFIG
                     VTGLISLMAVFYILRKK"
     gene            1245..1925
                     /gene="rpiA"
                     /locus_tag="VKL21_05620"
     CDS             1245..1925
                     /gene="rpiA"
                     /locus_tag="VKL21_05620"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HMB45281.1"
                     /translation="MKKSTQNPKQVAGEQAARLVQNNMVVGLGTGSTTAYAIKELGRQ
                     IANGLKIQGVPTSYQSAFLAVENGIPLTTLDEHPVLDIGIDGADQFANFIAIKGGGAA
                     HLREKIVAESAKKFVVILDESKMADVLNHSVPLEVIPCAYRLVERNVAKLGGKTKVRM
                     GVNKDGPVISDNGNFIMDADFGDIDDPVSLNKELSYCTGIVEHGIFTNVNVVYIGKKD
                     GTVEILES"
     gene            complement(1937..>2914)
                     /locus_tag="VKL21_05625"
     CDS             complement(1937..>2914)
                     /locus_tag="VKL21_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HMB45282.1"
                     /translation="GSMYGVHIEYVHEKEKLGTAGGVKNAEELFEGEPFMVVGGDHVM
                     DLELREFVRFHDKNDSLVTIGLLPIDDPHDYGIADLDVNNRIHRFFEKPGPGEIFSNL
                     ASTGIYVCDPEIMNWIPRKKYDFAKDLFPDIMKKGKNINGFLVHGKWTDIGNPQAYRE
                     ACRWMLEQMPGTKISGRLNIRDARVTGPIDIGHDVSLGSNSTIVGPIVIGENTTIGDK
                     VLIGPYTSIGSNCKIGNNSRILSSYIFNNVEIGSNTAVSSAIIDNGTKVKDSCILETG
                     TVIGPRVIIGEEVTVNSMVRIWPEVRVKNASKVIENLGNEDFGNNIKGS"
BASE COUNT          798 a          631 c          553 g          932 t
ORIGIN      
        1 ctaaggacct tacaaaaata aaagcaatta ttatgagagc taatatagtg aaagctctct
       61 ttaaatgtgg ctgcattact tatcaccttt ccatgacagg atatagtttc atcattgttg
      121 gcctttattt ctttctcaga atatagaaca cagccattag cgaaattaat ccggtaacac
      181 ctataaatcc tggtgctcct gctgcggcgg tttcagttgg cacaggagcg ggtttcacga
      241 tatcttcagc ctttttcact tccagattcg ctgcatttat ttgatcctcg aaatgggtca
      301 gattaaattc atgccacatt ggcgcaagat ttccaagtat cgcaagggca ttatccgtgt
      361 attgccctgc ctgtcccaca tcagtcccat tcttctgtgc ccagaaaata tcctgtcttg
      421 cattatctat attgaatttc aatttattta cttttgttag catatcttct gcttttaatg
      481 gtatgtcata aggcgctact ttcttataga cagaatgaca gttcccgcag gaattcctga
      541 aatcttcagg attcatgacc gtaaatgtat gaggaatatg gcatgttgtg catgttgggg
      601 cgagctgcaa tgattcaagc ttcttgtaat gcgtactatt ttcaaaatta tccagttctt
      661 ttgaatggca tttaccgcat gtctttggaa cattggtata ataaacaggg ctttcagggt
      721 cggtaatgtt cttgaagttg atatgtgctt tttctttggt agcctgtttc gggtctcccc
      781 catggcacgc atcgcatgtg actcctttca gggcatgaac cgattctgac cactgctgga
      841 aattagcggt cgcaagctgt cttaccctgt cttcatggca ttcaagagaa catgcggcat
      901 tcctttcaag atgctggatc cttatctggt taaaccgttt ttgttcttca ataaatggcg
      961 tcagtgtctt atgacaatcc acacatgaat tttcagacgc attcacatca ggaagaaata
     1021 caaaaattcc tgctaaaatt aataatacaa ttaagatttt attaaatgat accaatcgca
     1081 ttttattcaa accttccgtt ttaaacactt acgttttgct ttatatcgtt ttaaacttat
     1141 cgttttttct aatttcactt aacccgttat tttcttattt atatcttttg gtttcttttt
     1201 ttccaaatca tttatttaaa tataagacat taaagttgct catcatgaaa aaatctaccc
     1261 agaatcccaa gcaggtcgct ggagaacagg cagcccggct tgtacaaaat aatatggtcg
     1321 tggggcttgg cacaggttcc acaacggctt atgctataaa agaattggga cgacaaatag
     1381 ccaatggcct gaaaatccag ggtgttccaa catcatacca gtcagcattt ctggctgttg
     1441 agaacggcat cccacttact acacttgacg agcatcctgt ccttgatatt ggcatcgacg
     1501 gcgcagacca gttcgcgaat ttcatagcaa taaaaggcgg tggagcggct catttacggg
     1561 aaaagatcgt agcggagtca gcaaaaaaat ttgttgtaat attagatgaa tcaaaaatgg
     1621 ctgatgtttt gaaccattca gttccccttg aagtcatacc atgcgcttat agacttgtgg
     1681 aaagaaatgt cgccaaactc ggtggaaaaa caaaagtgag aatgggtgtt aataaggatg
     1741 gacctgtcat ctcggataat ggaaatttca ttatggatgc cgatttcggg gatatcgatg
     1801 atcctgtatc attaaataaa gaactttcgt attgcacagg cattgtggaa catgggatat
     1861 tcaccaatgt aaatgtggta tatatcggga aaaaggacgg aactgtagaa atattggaat
     1921 cttaagaaca tgaatcttaa gatcctttaa tattattccc aaaatcctca ttcccaaggt
     1981 tctcaattac tttacttgca ttttttaccc tgacttcagg ccatattctt accattgaat
     2041 ttacagtcac ttcttccccg attataactc tcggaccgat gactgtgccc gtttccagta
     2101 tgcatgaatc ttttacttta gtgccattat ctataatggc gcttgaaacg gccgtatttg
     2161 agccgatctc aacattattg aatatatatg aagaaaggat ccttgagtta ttgccaatct
     2221 tacaatttga gccgatcgat gtgtaaggac ctatcagaac tttgtcccct atagtcgtgt
     2281 tttccccaat tacaataggg cctactatag tggaatttga accaagggac acatcatgcc
     2341 cgatatcgat ggggcccgtt actcttgcat ccctgatatt caggcgccct gatatttttg
     2401 tacccggcat ctgttcaagc atccatctgc atgcttccct gtaagcctgc gggttcccta
     2461 tatctgtcca ctttccatgc acaaggaaac cattgatgtt tttgcctttt ttcataatat
     2521 ccggaaaaag gtcttttgca aaatcatatt tctttcgggg gatccagttc attatctccg
     2581 gatcgcaaac atatatcccg gtgcttgcaa ggttactgaa tatttccccg ggcccgggtt
     2641 tctcaaagaa ccgatggatc ctgttattta catcaagatc cgctatacca taatcgtgcg
     2701 ggtcatcgat ggggagaaga cctatggtta caagtgaatc attcttatca tggaaacgga
     2761 caaattcacg aagttcaagg tccatcacat ggtctcctcc cacaaccatg aaaggttcac
     2821 cttcaaatag ctcttcggcg ttctttactc cccctgctgt tcccagcttt tccttttcat
     2881 gcacgtattc tatatgcacg ccatacatac ttcc
//
LOCUS       DATGBN010000234         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_17595, whole genome shotgun sequence.
ACCESSION   DATGBN010000234 DATGBN010000000
VERSION     DATGBN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17595"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VKL21_05630"
     CDS             <1..381
                     /locus_tag="VKL21_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMB45283.1"
                     /translation="CTGGKIYSNSCSVIQALTEKGISVFIASGDSMRNLQPLAKNVCV
                     PLECVYEISTPKKKEGIVKILKEQYDKVVMVGDGINDILAFRAADLSVLSIQQTGKCP
                     PLLCEEVDIVINDIKEIIGIVENL"
     gene            complement(751..1932)
                     /locus_tag="VKL21_05635"
     CDS             complement(751..1932)
                     /locus_tag="VKL21_05635"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HMB45284.1"
                     /translation="MFLVGEALIGEAPELAHIDLIIGEKSGPVGQAFANGFTQLSMGH
                     TPLLSVVRPNLLAKPATLIVPKVTVKNMAQAAQIFGPAQTAVARAVADSVEEGVIPKD
                     QIEDLSIVVSVFIHPEAKDYNKIYRYNYGATKLAIERAMQGFPDVKTLMYEKDRTMHP
                     IMGFKVIRLWNPPYLQVAFDIPDIDAVKNILKQIPQSDHLIIEAGTPLIKRYGVNVVH
                     SIREARPNSFVVADLKTLDTGNLEARMVADATADAIVISGLAPISTIENAIKEARKTG
                     IYSIIDMLNVDKPMSILKALTVKPNVVELHRAIDTEGKSEHAWGDIQAMKKLSERMLV
                     AVAGGIRVDSVKEALKSGADIIVVGRAITKAKDVRSMTEQFIEEMKQPEIDQYRIMTD
                     F"
     gene            2027..2185
                     /locus_tag="VKL21_05640"
     CDS             2027..2185
                     /locus_tag="VKL21_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45285.1"
                     /translation="MKVYICEKSCCPAVEMIGDEVLIGEDTNIVKLKKNEWNKLVEKI
                     QSGELRSL"
     gene            complement(2182..>2723)
                     /locus_tag="VKL21_05645"
     CDS             complement(2182..>2723)
                     /locus_tag="VKL21_05645"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMB45286.1"
                     /translation="FVPERDQENAYGDYPLPIGCDQTISAPHMVAIMCDLLDIRDGMK
                     VLEIGAGSGYHAAVMAALAGSGHVYTVERIESLASLARDNLKKAGIKNVTVIVEDGSL
                     GFPGFAPYDRISVAAASPEILETLTDQLNYGGKLIIPVGKHYQELYLVTKTDGLKKEA
                     KGGVVFVPLVGKKGYSDWI"
BASE COUNT          757 a          602 c          550 g          814 t
ORIGIN      
        1 tgcacaggcg gcaaaatata ttccaatagc tgttctgtca tacaggcgct tacggagaag
       61 ggcattagcg tatttatcgc atctggcgat agtatgcgaa accttcagcc gcttgccaaa
      121 aacgtttgtg ttccacttga atgcgtatat gagatttcca caccaaagaa aaaagaaggg
      181 atcgtgaaga tattgaaaga acaatacgat aaggtagtaa tggtgggtga cgggatcaat
      241 gatatacttg ctttcagggc ggctgattta agtgttctct ccatacagca gaccggaaag
      301 tgcccgcctt tgctttgtga ggaagttgat attgttataa atgatattaa ggagataatc
      361 gggatcgtgg aaaatctttg aaaaacgtag acgagcgggc ggggagtggg agtggtgggt
      421 tatttaaaaa aatccgaccg ctcatcatat ataattatga ttatcatcat acttatactt
      481 tttcatgata caaaatcgat ggaatgacaa aataactcat aattatatcg aaaactttaa
      541 aactttataa gtgattcccc gaattttgcc cccagtgtag gtggcaggta atattttttt
      601 ctaaccccca ctccccaacc tgtcactgta cactaagatt atttttttat ggaaattttg
      661 aaaaaaatat tcgagggaag ggttttgaac cctcacatct ctgaaattaa ttttctattt
      721 tttctattat tagtattctt aactcaaata ctaaaagtcc gtcattattc tgtactggtc
      781 tatctcaggc tgcttcattt cttcaatgaa ttgctctgtc atggaacgca catcttttgc
      841 ctttgtgata gctcgcccaa caacgattat atcagcgcct gatttcagcg cttccttgac
      901 gctgtcaact cgaatgcctc ctgcaactgc cacaagcatc ctctcggata atttcttcat
      961 tgcttgaatg tctccccatg catgttctga ttttccttca gtgtcaatcg ccctgtgaag
     1021 ctccacaaca ttaggtttca ctgtaagggc ttttaaaatt gacatgggtt tatcgacgtt
     1081 aagcatatcg atgatcgaat aaataccggt cttacgcgct tcttttatgg cgttttcgat
     1141 agtggagata ggcgcaagtc ctgagatgac aatagcatca gcagtcgcat ccgcgaccat
     1201 gcgcgcctca agattaccgg tatccagtgt cttaagatcc gctaccacga aagaattcgg
     1261 acgcgcctcc ctgatagagt gcacaacgtt cacaccatat cttttgataa gaggtgttcc
     1321 cgcttctatg ataagatgat cgctctgggg tatctgtttc agtatgttct tgacagcatc
     1381 gatatccgga atatcaaatg caacttgcag ataaggcgga ttccataacc tgataacctt
     1441 gaatcccatt atcgggtgca tggttctgtc tttctcatac atcagggtct ttacatcagg
     1501 gaatccctgc atcgcgcgct cgattgcaag ctttgtggcg ccataattgt agcggtatat
     1561 cttattataa tcctttgctt caggatgaat aaaaacgctg accactatac tcaggtcttc
     1621 aatctggtct tttggtatta caccttcctc aacggaatct gccacagctc ttgccaccgc
     1681 ggtctgtgca ggcccgaata tctgggcagc ctgtgccata ttcttgactg tgacctttgg
     1741 cacaataagg gttgcaggtt ttgcaagaag gtttggcctc acaactgaaa gaaggggggt
     1801 gtgccccata gaaagctgcg taaaaccatt cgcaaatgcc tgtccgacag gtcctgattt
     1861 ttctccaatg atcaaatcaa tatgagcaag ttccggtgct tcaccgatta aagcttcacc
     1921 tactaaaaac attatcgtac ctcaggcctt aaataggtac tttggtattt atgtattttg
     1981 tagaaaacat taatatcttt gcatgccaac tgtacatggt gattaaatga aagtttatat
     2041 ttgtgaaaaa agctgctgtc cggccgttga aatgatcgga gatgaagtat tgatcggtga
     2101 agatacaaat atcgttaaat taaaaaaaaa tgaatggaat aagctggttg agaaaatcca
     2161 atccggtgaa cttcgttcac tctaaatcca atcactataa cctttttttc cgacaagggg
     2221 cacaaataca acgccccctt ttgcttcctt tttcaaacca tcggtctttg ttacaagata
     2281 gagttcctgg taatgttttc ctactggtat gatcaatttt cccccataat tgagctgatc
     2341 ggtcagggtc tcaagtattt caggagatgc tgcagcgaca cttatcctat catatggtgc
     2401 aaatccagga aacccaagag aaccatcctc tacgatcacg gtcacattct tgattcctgc
     2461 tttttttaag ttatcccggg ccaatgatgc aagggattcg attctctcta cagtatagac
     2521 atgcccgctg ccagcaaggg ccgccattac cgcagcatga tatcctgagc ctgcgcctat
     2581 ttcaaggacc ttcatcccat cccgtatatc gagaagatca cacattatcg caaccatatg
     2641 cggggctgat attgtttgat cgcaacctat cggcaaagga taatcgccat atgcattttc
     2701 ctgatccctt tccggaacaa aaa
//
LOCUS       DATGBN010000235         5865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_17645, whole genome shotgun sequence.
ACCESSION   DATGBN010000235 DATGBN010000000
VERSION     DATGBN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17645"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..281)
                     /locus_tag="VKL21_05650"
     CDS             complement(90..281)
                     /locus_tag="VKL21_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45287.1"
                     /translation="MEPVLSDAISRAKQTAIGMNYPASFGFNKRIRSQSGSLVNPATI
                     ISEAMDVVIRPDESHRELI"
     gene            complement(329..1525)
                     /locus_tag="VKL21_05655"
     CDS             complement(329..1525)
                     /locus_tag="VKL21_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45288.1"
                     /translation="MRTETTPSFITELPLKTTSADESTTLVRLDTGRQLYNACLGESL
                     NRLALIRQSKEFQEIQKLPKTIDGKQNKERTEAFKQLNKKHGFTDYDLQHYAVGIRNS
                     WIKEHIGVHIAQKIATRAFKAVQKKAFGIAKNVRFKGKNQFDSLEGKNNKTGLIFRNN
                     ILYWSGLEIPCIVDKDNDLISYGLKHRVKYCRIVRRKINARNRFYLQLILEGTPFQNP
                     ESKIGSEEIGLDVGVSTIGIVGDTKAELKQFCSELIPDQKKKRKLQRKLDRSLRASNP
                     DNYNKNGTVKKGSKNWIKSNRYKSLSNELSETDRILAAYRKSLHGRDINEILATGTRI
                     KTEKVPYKGWQKIFGRSMQVRAPSMFMSSLKRKAENAGGYLFEFPTQTTKLSQICHNF
                     WQNVLI"
     gene            complement(1522..1911)
                     /gene="tnpA"
                     /locus_tag="VKL21_05660"
     CDS             complement(1522..1911)
                     /gene="tnpA"
                     /locus_tag="VKL21_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HMB45289.1"
                     /translation="MQYKHSRNKVFLINYHLIWCPKRRKKVLVGEIKTRLEQIINEVA
                     KEKNTEILALEVMPDHLHLFVSSHPNILVHNLIKAFKARSSNLLRKEYPELLKLPSLW
                     THSYFISTERNVSSKTIKKYIEAQNTR"
     gene            2185..2652
                     /locus_tag="VKL21_05665"
     CDS             2185..2652
                     /locus_tag="VKL21_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45290.1"
                     /translation="MLEKSRDRVELLRKGIDGKKIEQLYLSSNNFVILQKNDPSQKVN
                     CWEFMNCGRELGGEKADKLGVCPVATDISADGLNRGKVGGRICWAISRKSGGKRKEGK
                     LAKKLLFCESCSFFKLVKEEEGGERFVSAKPENLKLTQEHFQKSQDPYQAHSS"
     gene            complement(2591..3871)
                     /locus_tag="VKL21_05670"
     CDS             complement(2591..3871)
                     /locus_tag="VKL21_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMB45291.1"
                     /translation="MIKKGIRSGEIISRDHKVSSPSLTRSYDLVVDHAEGSTIFDVEG
                     KSYIDFAASVAVMNIGYNCAKVKQAVCAQMEKMVHCGYSDFYAEEPVKLCEKLCEMTG
                     YEKVFLSNSGTETVEAAMKLAFWHTRKTSMIAFYRAFHGRTLGALSLTSSRIKHKEHF
                     PSLRVLHTDYAYCYRCPLNLEYPGCGISCAKEIERTIFKRELSPKDTAAIVVEPIQGE
                     GGFIVPPPEFHKEIRRICDENDVLMIADEIQSGGFRTGKFMALENFDVRADIVCMSKS
                     IGGGIPLGAMLSSGKIMNWPPGTHANTFGGNLLAAAGGLATLDFMESARLGEKALEKG
                     NYLIGRLNELKNKYPIIGDVRGIGLMTGVELVEENKDPAIEKRDIIIKRSLDGGLILL
                     PAGDSVIRFVPPLIIKKSELDKGLEIFENALGSI"
     gene            complement(3881..5353)
                     /locus_tag="VKL21_05675"
     CDS             complement(3881..5353)
                     /locus_tag="VKL21_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMB45292.1"
                     /translation="MQEYKLFINGEWTGSSSGEMFDDINPATLELIGKLHKANKEDVK
                     NAVDAAQDSFDAWSSTPAPQRARILFRAARMLEERKEELSRLMTMEMGKVLKEARGDV
                     QEAIDIAYYAAGEGRRLTGETTPSELPDKFCMTIRRPIGVVGLITPWNFPIAIPAWKI
                     MPALVAGNTIIFKPASDTPILSIELVNILKEAGLPKGVLNLVTGEGKTVGSEIVHNRK
                     IRAVSFTGSLETGKWIMGEASRDMKKVSLELGGKNPIIVMDDADLNLAIDGIVWGAFG
                     TTGQRCTAASRVIVQEKIYPHLLERLIGRTKKLKVGNGLHENIDMGPVINDSQLRKIA
                     KYVEIGKNEGAKLALGGNIIKPLPGYFFEATIFTDVSPGMRIAQEEIFGPVVSLIRAQ
                     DFDEAIHIANSVEYGLSSSIYSANMRHAFKAIEKIEAGITYINASTIGAEIHLPFGGV
                     KSTGNGTREGGTTAIEEFTELKTVYFDYSGKLQKAQIDIE"
     gene            complement(5474..>5865)
                     /locus_tag="VKL21_05680"
     CDS             complement(5474..>5865)
                     /locus_tag="VKL21_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45293.1"
                     /translation="KETLFIAAGDALTNKMLEEERKTAMKMQIIIIYVSFFIFLYVSH
                     SLVSGFLPQIPDVAAGSEVTGIIGEGITFSGIDKPMYERIFFHATILVGFFSGLVAGQ
                     IGEGDPRNGLKHSILMVLIGLGVFLQM"
BASE COUNT         1485 a         1305 c         1184 g         1891 t
ORIGIN      
        1 ttcaccataa cctttgagta aaggaggttc gtaggatgat cgtatttttg aaccctcttc
       61 cacggctgaa gccgggagat tcccagtgtt catataagct ctctatggct ctcatccggg
      121 cggattacaa catccatagc ctcacttata atcgttgctg gattaacgag cgaaccgctc
      181 tggctccgga ttcttttatt gaatccaaag ctggcaggat aattcatgcc gatcgcagtt
      241 tgtttcgccc ttgaaatcgc atcgctaagg actggttcca taccttgcca gagtagtttt
      301 gctctggcta tatcaagggt gcttgtttct atatcaacac attttgccag aaattatggc
      361 atatctgaga tagttttgtg gtctgtgttg gaaattcaaa taaatagcca ccagcatttt
      421 cagcttttcg tttgaggcta ctcatgaaca ttgagggggc gcgaacttgc atggatcttc
      481 cgaatatctt ttgccagcct ttgtatggta ctttttcggt tttgattcgt gttcccgttg
      541 cgaggatttc atttatatcc cgaccatgaa gggattttcg atatgcagct aggattctat
      601 cagtttcgga taattcgttg cttaatgact tatatcgatt tgattttatc cagttcttgc
      661 tacctttctt aactgtcccg ttcttgttat aattatcagg attgcttgca cgcaatgacc
      721 gatccagttt ccgttgcagt tttcgtttct ttttctgatc aggaattaat tcactgcaaa
      781 actgttttag ttcggctttt gtgtccccga cgattccgat agtacttaca ccaacatcca
      841 atccgatctc ttcgctgccg atcttacttt cggggttctg aaatggtgtt ccttcaagga
      901 ttaattgaag gtagaatcgg tttcttgcat tgatctttct tcggacaatt ctgcaatact
      961 tgacccgatg ttttaatcca tatgaaatta aatcgttatc cttatcgacg atacatggga
     1021 tttccagacc tgaccaatat aaaatattgt tcctgaatat caagcctgtt ttgttgtttt
     1081 taccttccag ggaatcgaac tggttttttc ctttgaatct cacgttcttt gctattccaa
     1141 aggctttttt ctggactgct ttgaatgctc tcgttgctat tttctgagcg atatgaacgc
     1201 caatgtgttc ttttatccag ctattcctta ttccaacagc ataatgttga agatcgtaat
     1261 cagtgaaccc atgtttttta ttaagttgtt tgaacgcttc tgttctttcc ttgttctgtt
     1321 taccgtctat ggttttcgga agtttctgta tttcttgaaa ctcttttgat tgcctgataa
     1381 gtgcaagacg gttcagactt tccccaagac aagcattata caactgcctt cccgtatcca
     1441 gacgtacaag cgtggtagat tcgtctgctg acgtggtttt taacgggagt tctgtgatga
     1501 acgatggagt agtttcggtt ctcatcttgt attctgagcc tcaatatatt tcttgatcgt
     1561 tttgctgcta acattccttt cggttgaaat gaaataagaa tgtgtccata gagaggggag
     1621 ttttagaagc tctggatact cttttctcaa gagatttgag cttctggctt tgaatgcttt
     1681 gatcagatta tgaaccagta tattcggatg agaggataca aataaatgaa gatgatccgg
     1741 cataacttca agggctaaaa tctctgtgtt cttctctttg gctacctcat ttatgatctg
     1801 ctctaatctg gtttttatct ctccgactaa tactttcttc cttcttttag ggcaccaaat
     1861 aagatggtag tttatgagaa ataccttgtt ccttgaatgt ttatattgca tgtttcatct
     1921 tcaataatgc ggttgctatt tatgtagtta tcgaccaaac taaggggttc gctatcctca
     1981 cggttgaaac cggaggcttg cgctcacccc ttaacctcta cggttttgtc agtattaatt
     2041 ttttttcatg agcaggatta gatatattaa tcagaatgat attctttatt atagatcatt
     2101 attttatttc attcaatgga taggaaaaat aatagggaga aaagagcggg gtaatctata
     2161 tatataaata ttggaagtgt atttatgtta gaaaaatcga gggacagggt agaactatta
     2221 agaaaaggta tagatggtaa gaaaattgaa caactttatt tatcaagtaa taattttgtt
     2281 atattacaaa aaaatgatcc atcacaaaaa gtaaattgtt gggagtttat gaattgtggt
     2341 agagaattag gtggagaaaa agcagacaaa ttgggcgtat gccctgtggc aaccgatatt
     2401 tctgctgatg gactaaatag gggaaaagtg ggaggaagaa tatgctgggc tatttcaaga
     2461 aaatctggcg ggaaaagaaa agagggaaaa cttgctaaaa agctattgtt ttgcgagtct
     2521 tgctcttttt ttaagttggt caaagaagaa gagggaggag aacgtttcgt ttcagcaaag
     2581 cccgaaaacc tcaaattgac ccaagagcat tttcaaaaat ctcaagaccc ttatcaagct
     2641 cactcttctt gattatcaaa ggcggcacga accttattac cgaatcacct gccggcagta
     2701 atatcagtcc cccatcaagt gatcttttga taataatatc tcttttttca atggctgggt
     2761 ccttattttc ctccacaagt tcaacgcctg tcataaggcc aataccgcgc acatcaccga
     2821 ttatcggata tttatttttc aattcgttca atcgtccaat gagataattt cctttctcaa
     2881 gggccttttc acccagtcgc gcgctctcca tgaaatcaag cgtcgcaagc cctccggcgg
     2941 cggcaaggag atttcctccg aatgtgtttg cgtgtgttcc gggcggccag ttcattatct
     3001 tgccacttga tagcatcgct cccagcggta taccgccgcc gattgattta gacatgcaga
     3061 cgatatccgc ccttacatca aaattctcaa gcgccatgaa ttttccggtt cgaaagcccc
     3121 cgctctggat ctcatccgct atcatcaata catcgttctc atcgcatatt cttctgatct
     3181 ccttatggaa ctcaggcggc gggacgataa acccgccttc tccctgtatt ggttcaacaa
     3241 cgatagcagc cgtatccttc ggggaaagtt cccttttgaa aatagtcctt tcgatctctt
     3301 ttgcgcagga aatgccacat cccggatatt caaggttaag aggacagcgg taacagtaag
     3361 cataatcagt atgtaagacc ctcaaagatg gaaaatgctc tttgtgcttg atcctggaac
     3421 tggtaagaga tagggcgcca agagttcttc catggaaagc ccggtaaaaa gctatcatgc
     3481 tggtttttct cgtgtgccag aaagcaagtt tcatggcagc ttcgactgtt tctgttcccg
     3541 aattagaaag aaatactttt tcgtaaccgg tcatttcaca tagtttttca catagtttta
     3601 cgggttcctc cgcataaaaa tccgaatagc cgcaatgaac cattttttcc atctgggcac
     3661 agacagcctg tttcactttc gcgcagttat atccgatatt cattaccgca acgcttgcag
     3721 caaaatcgat atagctcttt ccttccacat cgaatattgt cgaaccttct gcatggtcaa
     3781 ccaccagatc ataagagcgt gtaagggatg gcgatgaaac tttatggtca cggcttatga
     3841 tttctccgga tcttatgcct ttttttatca tgtttttttc ctattcaatg tctatctgcg
     3901 ctttctgaag ttttccgcta taatcaaaat aaacagtttt tagctctgtg aactcctcta
     3961 ttgccgtggt tccaccttca cgggtcccgt tccccgtgga tttgacaccg ccaaaaggca
     4021 aatgtatctc tgcaccgatg gtagaagcat tgatatatgt aatgcccgct tcaatttttt
     4081 caatagcctt gaatgcatgt ctcatatttg cgctataaat ggaagaagaa agaccatatt
     4141 ccaccgagtt ggcaatatgg atggcctcat caaaatcctg cgcccggatc aaggaaacaa
     4201 caggcccgaa tatctcttcc tgcgctatcc tcatgcctgg tgagacatct gtgaatattg
     4261 tagcttcaaa aaagtagcct ggcagcggtt ttatgatatt cccgcccagt gcaagttttg
     4321 cgccttcgtt ttttccgatt tccacatatt ttgcgatctt acgaagctgt gaatcattaa
     4381 taacaggccc catgtcgata ttttcatgaa ggccattgcc gacttttagt tttttggttc
     4441 tcccgataag tctttccaga aggtggggat agattttctc ttgcactatc accctgcttg
     4501 ctgcagtgca tcgctgcccg gtggttccga aagcacccca gactatcccg tcaatagcaa
     4561 ggttaaggtc cgcatcatcc ataacgatta tgggattttt tcctcccagt tccagcgaca
     4621 cttttttcat atccctggaa gcttccccca ttatccattt tccggtctca agagaacctg
     4681 tgaatgaaac cgcccggatt ttcctgttat gtacaatctc actgccaact gttttccctt
     4741 cacccgtcac aagatttaag acccctttgg gaagacctgc ttctttaagt atgtttacaa
     4801 gttcgatcga aaggattggt gtatcgcttg ccggtttgaa tataatcgta tttcctgcta
     4861 ccagggcggg cattatcttc catgcaggga tcgctatcgg gaaattccag ggtgttataa
     4921 gtccaaccac acctatgggg cgacgtatgg tcatgcagaa tttatccggg agttctgatg
     4981 gcgttgtttc acctgtcagg cgccttcctt ctcccgcggc ataatacgct atatcgatgg
     5041 cctcctggac atcccctcgc gcttctttga ggacttttcc catttccatg gtcatgagac
     5101 gtgaaagctc ttctttccgt tcttcaagca tccttgccgc cctgaataat atcctggcac
     5161 gctggggggc gggtgtgctg ctccatgcat caaatgaatc ctgggcggca tcaaccgcat
     5221 ttttcacatc ttctttattt gccttgtgca gtttcccgat caattcaaga gttgcagggt
     5281 ttatgtcatc aaacatctcg ccactacttg atcctgtcca ttctccattg atgaaaagtt
     5341 tgtattcttg catactcctc actcaattaa taagttgtag gctaatgtat tttaaccttg
     5401 gggcagagaa gtccccttct gaaagggagg gggataaaat actgaaaaag taaaaattgt
     5461 tgtaatcctt gtgctacatc tgcaggaaaa cccctaatcc tatgagcacc atcagaatgc
     5521 tatgttttaa accatttcgt gggtctcctt cgcctatctg cccagcaacc aggcctgaga
     5581 agaatcccac gaggattgtg gcgtgaaaaa atatcctctc gtacatgggc ttatctatcc
     5641 ctgagaaggt aatcccctcc cctattatcc ccgtcacctc cgacccggcg gcaacatcag
     5701 gtatctgggg aaggaacccg gatacaaggg aatgactaac atacagaaat atgaaaaatg
     5761 atacgtatat tattattatc tgcattttca tggcagtttt tctttcctct tccagcattt
     5821 tgtttgtgag agcatcccct gcggctataa aaagtgtttc cttga
//
LOCUS       DATGBN010000236         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_251197, whole genome shotgun sequence.
ACCESSION   DATGBN010000236 DATGBN010000000
VERSION     DATGBN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251197"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..328)
                     /locus_tag="VKL21_05685"
     CDS             complement(101..328)
                     /locus_tag="VKL21_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45294.1"
                     /translation="MNTVRVNITLPLEIAQMLRNVKNKSGFIAEAIRERIEREEEANL
                     IRELSEGYKARKKEDNELALEWSTTSGDGID"
     gene            complement(446..1777)
                     /locus_tag="VKL21_05690"
     CDS             complement(446..1777)
                     /locus_tag="VKL21_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HMB45295.1"
                     /translation="MLNKKTKALLIAGTASGVGKTTVAAGLMAALGRKYKVAPFKVGP
                     DFIDPGHHTQICGRSSRNLDSYIMGEKGVLETFERASKGADFCIIEGVMGLFDGLDST
                     EIASSAHIAKILDVPVMLVINAHGASRSIAAVVKGFSEFDSKVRIQGIILNNAGSQRH
                     IQLIKDSLHSTGIPVIGALPVNKEISIPSRHLGLYMAGEKNPKIAELANFIEQHIDMD
                     SLKTIAGNFEAHDIDSNVQNEQFNVKIGVANDSAFCFYYQDAFDALKMSGAELVFFSP
                     MSDSLPDVDGLYLGGGYPELFAKELEASDTRDEIKKAAQNGMPIYGECGALLYLGASL
                     ETDRTYRMAGVFGANSRMTAKLQALGYTEAQSIIDSPVAKKGAMIRGHEFHYSVTECD
                     RDARFVYRLKRGKGIFEGRDGLMEYNTLASYMHTHPASNSFGEFLTQCRKY"
BASE COUNT          456 a          456 c          330 g          577 t
ORIGIN      
        1 ttcattatta atattatttg agatcacgac cgctggtctt gtttttccta tctctgatcc
       61 ttcggtcgga tcgagtttga cccaataaac ttcgcctctt ctaatcaatt ccatcccccg
      121 atgtcgtact ccactcaaga gctaattcat tatcctcctt ctttcttgct ttataccctt
      181 cactcaattc tctgataagg ttggcttctt cttctctctc gatacgttcc ctgatcgctt
      241 cggcaataaa gccggattta tttttcacat ttcttaacat ctgtgcgatc tctaaaggta
      301 atgttatatt tactctgact gtattcatta tgcacctata ttaatgtatc aatggtgtgt
      361 atatatggta tgtatgatgc gcctgataaa tatttcggta atctgatcac ttgacccatt
      421 tttcagaaaa tatcctctat cttgatcagt attttctgca ttgtgtcagg aattcaccaa
      481 acgaattcga agccggatga gtatgcatat aacttgccag tgtgttatat tccatcaagc
      541 catcacgtcc ttcaaagatc ccttttcccc tttttaatcg atatacaaag cgcgcatccc
      601 tgtcgcattc cgttacagaa taatggaact catgccctct tatcatcgct ccttttttcg
      661 cgaccggtga atcgataatg ctttgtgcct cagtatatcc gagagcctgg agctttgcgg
      721 tcatccgaga atttgcgcca aacactcctg ccatcctgta agtcctgtca gtttcaagac
      781 tggcgcccag atacagaagc gctccgcatt cgccatatat gggcatcccg ttttgtgctg
      841 ctttttttat ctcatcgcgt gtgtctgatg cttcaagttc tttcgcaaaa agttcaggat
      901 atcctcctcc aaggtagagt ccatcgacat ccggcaaaga atcgctcatc ggactgaaaa
      961 atacaagctc tgcccctgac attttcaggg catcaaatgc atcctggtaa tagaaacaga
     1021 atgcgctatc attagcgacc cctattttaa cattgaactg ctcgttctgg acatttgaat
     1081 caatatcatg cgcttcaaaa ttccctgcaa ttgttttcag tgaatccata tcgatatgct
     1141 gttcaatgaa atttgccagt tctgcgattt tgggattctt ttcacccgcc atataaagac
     1201 caagatgacg tgacggaatt gaaatttctt tatttaccgg aagcgcccct atgacaggta
     1261 tcccggtgct gtgcagcgaa tccttgatta gttgaatatg tctctggctt cctgcattat
     1321 taagtattat cccctgaatc ctgactttgc tgtcaaattc agaaaaacct ttaacaacgg
     1381 cggctatact tcgcgatgcc ccatgcgcgt taataacaag catgacaggt acatccagga
     1441 tttttgcgat atgggctgaa cttgcgatct ccgtggaatc aagcccgtca aacaagccca
     1501 tgacaccctc aattatacaa aaatcagcgc cttttgaggc gcgttcgaat gtttccagca
     1561 cgcctttttc tcccattatg taactatcaa gattccttga ggagcggccg caaatctgcg
     1621 tatgatgtcc gggatcgata aaatcaggac ctactttgaa gggcgctact ttgtatttcc
     1681 tgccaagggc agccataaga cctgctgcaa ccgtggtttt tcccacaccg ctagcagtcc
     1741 ctgctatcaa aagcgccttt gtttttttat tgagcataat aaagacaggc aatcctccag
     1801 agtcccattc cctataata
//
LOCUS       DATGBN010000237         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_484023, whole genome shotgun sequence.
ACCESSION   DATGBN010000237 DATGBN010000000
VERSION     DATGBN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484023"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..457
                     /locus_tag="VKL21_05695"
     CDS             254..457
                     /locus_tag="VKL21_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45296.1"
                     /translation="MKTTVQTLKSRIDTLEAEIIAMLRVLGATMEKKDSKAWDRLHKI
                     GKQISLEWKSDKTSWQLISEARR"
     gene            499..885
                     /locus_tag="VKL21_05700"
     CDS             499..885
                     /locus_tag="VKL21_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMB45297.1"
                     /translation="MKSEKLHEYSEKLMEKIREKGITILVPEIVLPEISSAIARGSNN
                     SEKALEFVKELKLIPNIVFVPIDRELADEAARFAAEYRLRGCDAIYVAVASMFSVKLI
                     SLDEQQLVRAKDCIDASTPQDELKNI"
     gene            1368..2138
                     /locus_tag="VKL21_05705"
     CDS             1368..2138
                     /locus_tag="VKL21_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMB45298.1"
                     /translation="MNIFCITNNKGGVAKTTSAVNLAYALANNGNKILMIDTDPQCNS
                     TYSLLGNLDQSNTLYEVLLDNLPIEQTIIPTKHKNLFVVPCSINLSAADLMLASAHGR
                     ERKLERAIRPIKDSYDFIIIDTPPHLGVLTINALIACTDVLIPFSLTTYSLIGVGILE
                     KTIQELRDNLDVKLPILGVFACLDDHTNINKNVLHAIKDMFKEKVFRTVIPRNVALEE
                     AHNLTISIFDYQPASKGARAYEDLTKEILERVNLTYGK"
     gene            2128..2436
                     /locus_tag="VKL21_05710"
     CDS             2128..2436
                     /locus_tag="VKL21_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45299.1"
                     /translation="MGNKKTFINPLTRSSEEDLNIPPTPTPSVTPTPTSNSPTPNEYP
                     SLRKRGNKAFEKTHERFTGWMDKQLKKQFVNLAQNKGVSKASLLNEAIADLLRKHMQK
                     "
BASE COUNT          954 a          538 c          601 g          773 t
ORIGIN      
        1 tttatggtgt atatcgtctg ccataaaaaa atagaatgat gaaaaaatgg tattattttg
       61 atagatcctg ccgagattct ccggggagat ccaccgtagg cctcaaagtg agaggttgga
      121 ggtctggggg aaggggagag gaagaagaga taaatgaatt aaaaatctca tattttcaac
      181 tgaaagtatt tttcagctta agtgaatatt tatattgtat caatcaataa atgtttatga
      241 attcggagaa acaatgaaaa ctactgtgca gactcttaaa agccggatag acaccctcga
      301 agcagagatt atcgctatgc taagggtgct tggtgcaaca atggagaaga aagattcaaa
      361 agcctgggac agattacaca agattggcaa acaaataagc cttgaatgga agagcgataa
      421 aacaagctgg cagttaattt cagaagccag gcgatagtgt gtattgcata gatgcaagcg
      481 tactaataaa ctctgagatt gaaaagcgag aaattacatg aatatagcga aaagctcatg
      541 gaaaaaatca gagaaaaagg gatcactatc ctcgtccctg aaattgtcct gccagaaata
      601 tcttctgcaa tcgctagagg ctcgaataac tctgaaaagg cactggagtt tgtaaaagag
      661 ttgaaactaa tccccaatat tgtgtttgtt ccaatagaca gagaattagc agatgaagcc
      721 gcgagatttg ccgcagaata cagactaaga ggttgtgatg caatttatgt tgctgttgca
      781 tccatgttca gtgtaaaatt aataagcctt gatgaacagc aacttgtgag ggcaaaggat
      841 tgtatagatg catcaacacc acaagatgaa ttaaaaaata tctgaaattt ggtctcttga
      901 tattcatatc aatcgatagt ggcctctaat tattatatga aaatggcttt gtatgcccaa
      961 gggaacctgg ataagaattc gaaacaaatc cggtaggact aatcttttgt taggcttaca
     1021 gtaatgctgt catcagatgg tgaaaatgaa gatatctttg aatgatgggt ctaaagttgc
     1081 gctgagttcc actctacatc ttatgcacat atcatgtcga gtggaaaacc aagacagctt
     1141 cactttaagg ggtaatagtc gagcttctgc atcctggggc cacttcacag caacgcttaa
     1201 aaaagatact gctgaagcgg aagcaattga gcggattaag ataccagctc gatttgtccg
     1261 taaagtacct acaagggtat ttttgagcaa aaccatgaaa aggtgaagct tagtatatat
     1321 agtatgtagc cgtctacgta tacgtagtat aaagggatta taaaattatg aatattttct
     1381 gtatcacaaa taataaaggc ggtgttgcta aaactaccag tgcagtaaac cttgcctacg
     1441 ccctggcaaa taacggcaat aaaatcttaa tgattgatac tgatccacaa tgtaattcta
     1501 cttactcctt acttggaaat ctagaccagt caaatacact ttatgaagtc cttctggata
     1561 atcttcccat tgaacagaca attattccga caaaacacaa aaacctgttt gttgtcccat
     1621 gctcaattaa tttaagcgcg gcggatttaa tgttggcttc agcacatgga cgggaacgaa
     1681 aattggaacg agcaatcagg ccgataaaag attcatatga ttttattatt attgatactc
     1741 ctccacacct gggagttttg acaatcaatg cgctcatcgc ctgtactgat gtcttaattc
     1801 ctttctcgct gactacctac tcacttatcg gggtcggtat tctagaaaag accattcaag
     1861 aacttagaga taatcttgat gtgaaattgc cgattttggg agttttcgct tgtctggacg
     1921 atcacacaaa tatcaacaaa aacgttctgc atgccatcaa ggacatgttt aaagaaaaag
     1981 tattcaggac tgttattccg agaaatgttg cgcttgaaga ggcacataat ctaactattt
     2041 ctatttttga ttatcaacca gcgagtaaag gcgccagagc atacgaagat ttaaccaaag
     2101 aaatattaga gcgggtgaac ctgacctatg ggaaataaaa aaacatttat caatccactc
     2161 acaagatcaa gcgaagaaga tctgaatatt ccaccaacgc caacaccttc agtaacaccc
     2221 acaccaacaa gcaactcccc taccccgaat gagtatccat cactccggaa acgcggtaat
     2281 aaagcctttg aaaaaacgca tgagcgcttt accggttgga tggataaaca attaaagaaa
     2341 caatttgtaa accttgctca aaacaaaggc gtttcgaaag cttctctctt aaatgaagcg
     2401 attgcagacc tgctaagaaa acacatgcag aagtaagagt tttcttctac gtttgcgtat
     2461 atgtggacgt agacgtatac gttgatgttt cataaccaga ttagttccct ctcggtgaga
     2521 ggggttgggg tgcggggaag ggagagagga gatgataaaa gcaaggatct tatccacatg
     2581 ctttcaggag tgctcgattt tttctcgtgc ccaaagatag agttccaagc tcatcctaaa
     2641 gtctcccgaa gataatttat taattgtctc taaagcgagg tcccttgtta ttttttgaga
     2701 tccagcagca gcaacaatga gtccaagtaa acctgtgaac tttaatcctt ttgactcggc
     2761 aaaccttctc gcatttgcct catcaagcac aagtcgatca ttggcttctg ctgcaaggaa
     2821 cagacttggt tcctactttg caatgaaggc attccaggga aggggg
//
LOCUS       DATGBN010000238         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_329144, whole genome shotgun sequence.
ACCESSION   DATGBN010000238 DATGBN010000000
VERSION     DATGBN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329144"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..610)
                     /locus_tag="VKL21_05715"
     CDS             complement(176..610)
                     /locus_tag="VKL21_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45300.1"
                     /translation="MFIDDLSAAIPIVGLILVIIIGIIFIFKLKKMVFLFSPSFYAII
                     AGALIGAWINLLTGLVFSPRDQNIIKILFMAVCIFMSSITIIYISFDLEKLNRRVSTE
                     VSEKENSIRLDELIPEQNRIIYLLAISWIFLIIGMFQIYYIL"
     gene            complement(944..1846)
                     /locus_tag="VKL21_05720"
     CDS             complement(944..1846)
                     /locus_tag="VKL21_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HMB45301.1"
                     /translation="MVDTIIEGSNQKRTYVTLLVSDGYLPGILVLAYSLRVVGSRYPF
                     LVIATPNISQTVFAALDKQDVKYKIVEPITYTFLVRPLSLRDSSLGRVPKIDSRFLMT
                     YTKLRIWELVEFDKMVFIDGDMLVCSNIDDLFDKQVWSAVNAGGELLAFRHWVDLNSG
                     LMVIEPDKALFSDMMGKINMLHFKDYGDQGFLHTYFPEWPKRKELHLPHGYNMAVGHI
                     DSYVKELGYGWPYSSASEEKKVKALHYWGKDKPWVIPLDKMNLQLANEKPYYFESMML
                     WYQHYCELMSNFGSIPRMLRHYHS"
     gene            2164..>3035
                     /locus_tag="VKL21_05725"
     CDS             2164..>3035
                     /locus_tag="VKL21_05725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HMB45302.1"
                     /translation="MFSVLYQTFQDFEIIVVEGSSNDNSADVVRNFDDSRIRLIQQEN
                     PGVSVARNLGIDEAKAELIAFLDADDEWLPTFLETILRLRKKYPEAGAYATEYKNILI
                     KEKEVKFYGLPKGQSEGLLPSYFKSAVICKNIVRTSFVAIPKKIFTEVGKFAIGVWWG
                     EDVDMWGRIALKYPVAFCSDCKGIYHTDASNRACNRTEPVRRQIFVTSALKALHAGEV
                     PLELKEDLLEYIASKEIQTACRNLKAGRPDLARSNLENLNTKYFKKSFYWILFWTYVP
                     SKIFISLRNLKLKL"
BASE COUNT         1022 a          551 c          535 g          927 t
ORIGIN      
        1 tactatataa agaatcataa agttgctgtc tgctgttttt tgaaccttca atgattaaaa
       61 acttcttttc cttaattttt tcgacatatt taagatattc ttgattgatt tctgaattct
      121 ccaacgattg ttcaatttta taattttgaa attttttccc catagaatca cctttttata
      181 aaatataata tatttgaaac attcctataa taagaaatat ccaactaata gctaataaat
      241 aaataattct attttgttct ggtatcaatt catctaaacg tatagaattt tctttttcag
      301 aaacctcggt agatactctt ctattcaatt tctctaaatc aaaacttatg taaataattg
      361 ttattgacga cataaaaata caaacagcca taaatagaat ttttattata ttttgatctc
      421 tagggctaaa aaccaaaccg gtcaataaat taatccatgc accaatcaaa gctcctgcaa
      481 ttatcgcata gaaagatggc gaaaacaaaa aaaccatttt ttttaattta aaaataaata
      541 ttatacctat aataattact aaaatcagtc ctactatagg aattgctgca ctcaaatcat
      601 ctatgaacaa taatatccgc ctttatggaa ttattcttga tttcgtcagt tctcatatta
      661 aaagagatat tcggtgattt cttgcccata tattttgaag gaactaaacc gaaattgtat
      721 gctttagaat actcaaagga tggtgcacct atggaaaaaa aatcacattt ggaatcaatc
      781 tgatgtcaat aacattctga cagcattgac tgacattaaa tgagtattca atcatcttcc
      841 aaaccacctt ctgatgatac attttttcaa ttagcctcca gtatattgaa tatagataca
      901 atataatgac atttaaaacc tacgataccg ccctgcattc tcactacgaa tgataatgtc
      961 ggagcattct aggtattgac ccgaagttac tcatcagttc gcaataatgc tgataccaaa
     1021 gcatcatgct ctcgaagtaa tacggcttct catttgctaa ctgcagattc attttatcta
     1081 ggggaataac ccatggttta tccttccccc aatagtgtag agctttgacc ttcttttcct
     1141 cggaagcgct cgaataaggc caaccgtatc ccaattcctt cacataagaa tcgatatgac
     1201 cgactgccat gttgtaccca tgaggaagat gcaactcctt gcgctttggc cattccggaa
     1261 agtatgtgtg aagaaaacct tggtctccat aatctttgaa gtgcaacata ttgatcttgc
     1321 ccatcatgtc ggagaaaagc gctttgtcgg gttcaataac cattaaccca gaatttaggt
     1381 cgacccagtg gcgaaaagcg agaagctcgc caccagcatt cacagcagac cagacctgct
     1441 tatcaaataa gtcatcaatg ttgctacaaa caagcatgtc cccgtcgatg aagaccatct
     1501 tatcgaactc cacgagctcc catatgcgca gcttagtata tgtcatcaga aaacgggagt
     1561 cgatttttgg cacgcgcccg agtgaactat ccctcaatga cagaggcctt acaagaaacg
     1621 tgtaggtaat aggctcgaca atcttgtatt taacatcctg tttgtcaagt gcagcaaaaa
     1681 cagtctgaga gatattaggg gttgcaatga caagaaacgg gtagcgcgag ccaacgactc
     1741 gaagagaata ggctaaaacc agtataccag gaagatagcc atcgctaact aaaagggtga
     1801 cgtaagttcg tttttgattc gatccttcaa ttattgtatc gaccacgata ctaatatgta
     1861 taatatcctt aatatttttt ctaaataatt ggtcgtgtag tgaactattt aaaaaaataa
     1921 tttaggtgca atgcttagca ctagaaagat tatataatct tcaaaacaat agatgttgct
     1981 tataaagtct tttaagaatg aatcatatgt caaaacacat gaaaatatat ccaaatcttt
     2041 ttcaaatatt acacaaagga tgtttgcatt attaattccg aaaatcgtaa tggactaaag
     2101 agaatttgta gcgtctcggt aattatccca ctttacaaca aaggccccta tatagagaga
     2161 gctctgtttt ctgttcttta tcagacgttt caggattttg agataattgt agtggaaggc
     2221 agctcgaatg ataatagtgc tgatgttgtc aggaattttg atgattcgag gattcgattg
     2281 atccagcaag agaatcctgg ggtttctgtt gcgaggaacc ttgggataga tgaggcaaaa
     2341 gcggaattaa ttgctttttt ggatgcagac gatgaatggt tgcctacatt tctggaaaca
     2401 attcttcgtt tgaggaagaa atatccagaa gcaggagctt atgcgacaga atacaaaaat
     2461 atactcatta aagaaaaaga ggtaaaattt tatggtcttc caaaaggaca atccgaaggc
     2521 ttacttcctt catatttcaa gtctgcagtc atttgcaaaa atattgttcg gacatcattt
     2581 gtcgcaatac caaagaaaat atttactgaa gtaggtaagt tcgccatagg agtatggtgg
     2641 ggtgaagatg tagatatgtg gggtagaatc gcattgaaat accctgtggc attttgcagt
     2701 gattgtaaag gaatatacca cacagatgcc tcaaacaggg catgcaatag gacagaaccg
     2761 gtaagaagac aaatatttgt aacttcagcc ttgaaagcat tgcatgctgg tgaggtgccg
     2821 ttggaattaa aggaggatct tctggagtac atcgcgtcaa aagagatcca gacagcttgt
     2881 cgaaatctaa aggcaggtag gcctgattta gcaagatcta atctagaaaa tttgaataca
     2941 aaatatttca agaaatcttt ttattggatc ttattctgga catatgtccc ttcaaagata
     3001 tttatatccc taagaaatct gaaactaaaa ttgta
//
LOCUS       DATGBN010000239         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_18075, whole genome shotgun sequence.
ACCESSION   DATGBN010000239 DATGBN010000000
VERSION     DATGBN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18075"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..965
                     /gene="porB"
                     /locus_tag="VKL21_05730"
     CDS             93..965
                     /gene="porB"
                     /locus_tag="VKL21_05730"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HMB45303.1"
                     /translation="MSLLKPGHRGCAGCCDALAGKFMLDALGEDVIVVSPTGCLEVFT
                     TPYPESAWGVPWVHSLFENAAAVASGVEAALKALGKKNNTKVVAIGGDGATLDIGLQA
                     ISGAFERGHDITYICVDNEAYMNTGIQRSSATPLYASTTTSPAGKVSIGNPQHKKNMP
                     AIIAAHGSPYVATASIAYAPDMMKKVKKAAQIKGPTYVHVHAPCCTGWKFEGSKTIEV
                     GRLAVETAMWPLYEMENGEITGVRKIKNRKPVEEYLKVQGRFKHLFTMEGRTEDIKKI
                     QAIADWNTKHFGLE"
     gene            1012..1320
                     /locus_tag="VKL21_05735"
     CDS             1012..1320
                     /locus_tag="VKL21_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMB45304.1"
                     /translation="MTESIILKDHIEYNTGSVVSKTLKQNPAGSITLFAFDAGQGLSE
                     HSAPFDAVVQVIDGEGQFIIGGDEHVLKEGELIIMPANVPHAVNARQKFKMMLIMLKA
                     "
     gene            complement(1574..2074)
                     /locus_tag="VKL21_05740"
     CDS             complement(1574..2074)
                     /locus_tag="VKL21_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45305.1"
                     /translation="MNKKTVTVLLLLALMIFIGVPTSFAIPQYRANLTEVYGAGSCET
                     CHNNGSADGPRTSYGMLFENQTNHVANASAALIAIGAPPKSTAATPTAAITTTPIKVT
                     PEITAETTEDTQEDTQGNTQEDTESQEETFPVTTEEAKGSPGFGFMASLVGLFAWALL
                     TKRNNK"
     gene            2343..>3849
                     /locus_tag="VKL21_05745"
     CDS             2343..>3849
                     /locus_tag="VKL21_05745"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HMB45306.1"
                     /translation="MRILIVSNRLPFTVVEKEGTFRYQESVGGMVSGLSGYIDSMKTS
                     SFVELEYIWIGWPGITIEDKKQQEELKSKALSDFHAYPIFLSRKTMDKFYYGFCNKTL
                     WPLFHYFSSYTVYEEDSWRYYRHVNEKFCEALMEIIKPDDIVWVHDYHLMLLPGLLKK
                     KFPDNPVGFFLHIPFPSFEIFRLLPKKWQKELLEGMLGADLVGFHTHEYTQYFLRCVL
                     RIMGYENNMGKIITQNKIVKADTFPMGIDFRKFHDHTHSVQVQKEEEKFDRMLANYKI
                     ILSIDRLDYTKGLINRLLSYEIFLERYKQWHKKLILLMIVVPSRIGVEYYQQNKKEID
                     ELVGRINGRFGSIDWTPISYQYKFLPFSSLVALYKRSDIALITPLRDGMNLISKEYIA
                     ARTDKTGVLILSEMAGASKELGEAIIINPNNTEEITEALKEALEMPKEEQIKRNHIMQ
                     ERLKCYDIVRWAHDFIEGLLSVGDEQKRFDSRLLNHKVKEEMKVDFNKARKR"
BASE COUNT         1191 a          720 c          843 g         1095 t
ORIGIN      
        1 cacatgatca agaaaatcat caacaaggca aaattagttg aaaaaggcat ctttgtggaa
       61 agcgaatatg ccgacctgag ggaggacctg gcatgagcct gctaaaaccc ggtcatcggg
      121 gctgtgcagg ttgctgcgac gctcttgcag gaaaattcat gcttgatgcg cttggtgaag
      181 atgttatagt tgtcagcccg acaggatgtc ttgaagtatt taccacacct tatcctgaat
      241 ctgcatgggg tgtgccatgg gtgcattcac tttttgagaa tgcagccgct gttgcttcag
      301 gcgttgaggc cgcattaaaa gcgcttggca aaaagaataa cacaaaagtg gttgcaatag
      361 gcggagacgg cgctaccctt gatatcggct tacaggcaat ttcaggcgca tttgagcgcg
      421 ggcatgatat aacctatatc tgtgtggata acgaggccta tatgaatact ggtatccagc
      481 gaagcagcgc aacaccgtta tatgcaagca caaccacaag ccctgccggc aaagtctcta
      541 tcggaaatcc ccagcataag aagaatatgc ctgcgataat agcggctcac gggtcgccat
      601 atgtggcaac ggcatccatc gcatatgcgc ctgatatgat gaagaaagta aaaaaggcag
      661 cccagataaa aggcccaact tatgttcatg tccatgcgcc atgctgcaca ggatggaaat
      721 tcgagggctc aaagacaata gaggtgggca ggttagcagt tgagactgca atgtggcctc
      781 tgtatgagat ggaaaatggc gagatcacag gagtaaggaa gatcaaaaac aggaaacctg
      841 tagaagaata cctgaaagta caggggcgct tcaagcatct tttcaccatg gaaggcagaa
      901 ccgaggatat aaagaagata caggcgatag ccgactggaa tacgaaacat ttcggcctgg
      961 aataagtata ttcagcatca ttttaatacc tgaatcctca ttgttattct catgactgaa
     1021 tcgatcatat taaaggacca tatcgaatat aataccggat cagtggtcag taagaccttg
     1081 aagcagaacc ctgcaggttc cataacctta tttgcttttg atgcaggaca gggattaagc
     1141 gagcattcag ctccttttga tgcagtggtc caggttattg atggggaggg acagttcata
     1201 atagggggag atgaacatgt tttgaaggaa ggagagttaa taataatgcc ggcaaatgtg
     1261 cctcatgccg ttaatgcaag gcagaagttc aagatgatgc ttataatgtt gaaggcatga
     1321 atctgaatac ttatttcaaa cgtgacctac tcccacgatt taaatcgtgg gcatctgctt
     1381 atgtacttgc atggtttttc tctccatgtt catctacact ttcagccagt catctcggca
     1441 tcgaaggaaa gtattttcct tcacaggatg cgctttttaa gcaacacggt tgcaagagcg
     1501 aatccacgca gatttttatt ttgatttttt tttccactta tgtccaaatc tgccaatcga
     1561 atattcaact gaattattta ttatttctct ttgtcagaag tgcccatgcg aacaatccca
     1621 caagggatgc cataaatcca aatcctgggg atccttttgc ttcctcggtt gtaactggaa
     1681 atgtttcttc ctgtgattca gtatcttctt gagtatttcc ttgagtatct tcttgagtat
     1741 cttctgtagt ttctgcagtt atctcaggtg tcacttttat tggtgttgta gtaattgcag
     1801 cagttggcgt tgctgcagtt gatttaggcg gcgcacctat agctatcagg gccgcactgg
     1861 cattagcaac gtggttagtc tggttctcaa ataacattcc gtaagaggta cgcggcccat
     1921 ccgcagaccc attgttatgg caggtttcgc atgaaccggc gccatacact tcagtaagat
     1981 ttgcgcgata ctggggtata gcaaaagatg tcggtacccc tataaagatc attaatgcta
     2041 acaaaagtaa cactgttact gttttcttat tcatatattg ttcctccttt ttatctccca
     2101 ttactattat tttatgaagt agggagtaag aaatgaatgg ttttgaatcc tatataatct
     2161 ttgctaattc tgctgatttt tatgggtgct ttcactatta aattgtagaa ccagataggt
     2221 atattaaaca tcggatatta tattaggata aattgaatat gattattaag tatatattta
     2281 tttatgaata tactcacaat ttcaagaacc ggacttattg agctttatat aggatgcaat
     2341 tcatgagaat tctcatagtt tcaaatcgac tgcctttcac tgttgttgaa aaggaaggta
     2401 cgttcagata tcaggaaagc gtaggcggca tggtgtcagg tctttcagga tatattgatt
     2461 caatgaaaac atcttcattt gtagagttag aatatatctg gattggatgg ccggggataa
     2521 ctattgaaga taaaaaacag caagaagaat taaaatcaaa agcgctttct gattttcatg
     2581 catatcccat atttctttcc aggaaaacaa tggataaatt ctactatggt ttttgcaaca
     2641 aaaccctctg gccgctattt cattattttt cgtcttatac tgtatatgaa gaggactcat
     2701 ggagatatta caggcatgta aacgaaaaat tctgcgaagc tttaatggaa ataataaaac
     2761 ctgatgatat cgtctgggtc catgattatc atctcatgct tcttccaggg cttctgaaaa
     2821 aaaagtttcc tgataatccg gttggatttt ttcttcatat cccttttcct tcgtttgaaa
     2881 ttttcaggtt gctccccaaa aagtggcaga aggaattact ggaagggatg ctgggagctg
     2941 acctggtggg attccatacg catgaataca ctcaatattt cctgagatgt gttctgcgca
     3001 taatgggtta cgagaacaat atgggcaaga ttataacaca gaataagatc gtaaaagctg
     3061 acacatttcc catgggtatt gatttccgga aattccatga tcatacccat agtgtgcaag
     3121 ttcagaaaga ggaagaaaaa tttgacagga tgcttgcaaa ctacaaaata attctttcta
     3181 tcgacagatt ggattatact aaaggtctta tcaaccgtct tctcagctat gagatcttcc
     3241 ttgagagata taagcaatgg cataaaaaat taatacttct gatgatagtt gttccttccc
     3301 gcattggggt agaatactat cagcagaata aaaaagaaat agatgaactt gttggcagaa
     3361 tcaatggaag gtttggcagc atcgactgga caccaatatc ctaccagtac aagttcctgc
     3421 cattcagttc tcttgtggct ctttataaaa gaagcgacat tgcattgatc acgcctttaa
     3481 gagatggtat gaatctcata tcaaaagaat atattgcagc aagaacggat aaaacaggtg
     3541 ttctgatact cagcgaaatg gcgggggctt caaaagaact cggagaagct atcatcataa
     3601 atcccaataa tacagaagaa atcactgaag cattgaaaga agcactagaa atgccaaagg
     3661 aagagcagat caagagaaac catatcatgc aggaaaggct caaatgttat gatattgtaa
     3721 gatgggcaca tgatttcatt gaaggtctgc tttctgtagg cgatgaacaa aaaagatttg
     3781 actcaagact attgaatcac aaagtcaagg aagagatgaa agtagatttc aataaagcca
     3841 gaaaaaggc
//
LOCUS       DATGBN010000240         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_212722, whole genome shotgun sequence.
ACCESSION   DATGBN010000240 DATGBN010000000
VERSION     DATGBN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212722"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VKL21_05750"
     CDS             <1..288
                     /locus_tag="VKL21_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobN"
                     /protein_id="HMB45307.1"
                     /translation="VVEPGARSIETAGRIAELGRQIGISKFGVVINKAAEEVLEIENK
                     LKGYDLPVIGSIPFDPDMVKADLDNISPIERGGAAISSIIKIFERLSNKME"
     gene            285..977
                     /gene="radB"
                     /locus_tag="VKL21_05755"
     CDS             285..977
                     /gene="radB"
                     /locus_tag="VKL21_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HMB45308.1"
                     /translation="MKSIKKPERIPSGCISLDEMLGGGFETGIVTQIYGGSGTGKTNI
                     CIQLTVECVNSGKKVIFIDTEGFSGERFKQIAGEDAKKIAGEIIIYEPASFEQQYSAI
                     TDIEKLMNEKIGLIILDSAALFYRLGLTQDETGEENVATRRELVNQIGILHGIARKYG
                     VAVVITNQVYKDVATGELCPVGGNAFEHLSKTIILLDKTGPSKRRATLRKHRSKGEGA
                     FCEFMLIDKGAK"
     gene            complement(989..1300)
                     /locus_tag="VKL21_05760"
     CDS             complement(989..1300)
                     /locus_tag="VKL21_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HMB45309.1"
                     /translation="MKTRLLKFLVRDKIGIRKCLLNLFLQTRNYTTCEVYDYLKKQGF
                     EVNYRGVSAMVGQMHTRLGILRIYLKREHRCYSLKEDRRDIVKMILTTPFSGKSLHSI
                     P"
     gene            complement(1395..1766)
                     /locus_tag="VKL21_05765"
     CDS             complement(1395..1766)
                     /locus_tag="VKL21_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45310.1"
                     /translation="MILIPLFLVFILPDEATGFFSIIQFSYSGIQIFNLHILLLVWSA
                     FIGIISYAEVLSWYLLREPEPAPVIKEAEPSAIKEGAPPAIKEAAPVAGDKPKTLMFK
                     VNDFSQKLYKILKGEKIKLKP"
     gene            complement(1859..3499)
                     /locus_tag="VKL21_05770"
     CDS             complement(1859..3499)
                     /locus_tag="VKL21_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45311.1"
                     /translation="MMNIKIIICFILIICFFPLASFGATEKVWSAKFSGIIKSNQSIV
                     FENYLVKAKALDNTKSSIIVYKDNLQLEKKDFNINEFKKYDAVGITLLGINGEYSWIA
                     ISKLEDRDIWISSGQKTLKWGETYSFEDVSIGIESLGKDSVNLTVSRNNTVTTDFFTI
                     NGFKNYDNMRIVVKDINRTGLIDIELFKFRIPTFEANINTDKEEYFPDEKISVSINIT
                     GDELLNIASISLDGSNSLEFQPDVFTAIDLDGTRSFHSQIAELPPNSTLTINGKIELR
                     DYHNNVYFANVSKEVSVAPNISIIKRVQEETDDETVLVELSIYNSGSKKTFVYIHDDI
                     TDGKNTSQKDWNIELGPKKSTNVSYFISPQKPGIYQLASAIAKWNGEISTSKKVKTTV
                     HMPYLSIVKTSLNNESMTDVELEIKNVGDRPAIVKVDDKIPDGFLLESGSPTWSGYID
                     SGKSANLRYSLKGNPVSLPPANATYRDIRGTVRQAQSNAIENNNIQGTKKIDTTHINA
                     SGYEIMVFMMSSFLVISGIIGSITFTAYLITKNRTRSK"
     gene            complement(3562..>4703)
                     /locus_tag="VKL21_05775"
     CDS             complement(3562..>4703)
                     /locus_tag="VKL21_05775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMB45312.1"
                     /translation="PPSYPSNGLRPKTMIISHGHLDHCGLAPNLMDLGLEVYCTSLTS
                     KLSALLARDTLKIARKKGHNIPYYNEDIQEFERKARSTEYRKEFNTNGYSVCFYDAGH
                     IPGSSMVHLEKDDQSIFYTGDMNTINTELQNGADTDLPDSDILLIESTYFGKHHTDRK
                     ILEERFIGSIRETIESGGRAIIPAFGIGRTQEIMLILKKHGLRAYVDGMGVDVFNLIK
                     RSPEYVPDIKKLENAFTSANIIERQDRAAIIEEPSIIVTSAGMLNGGPVLYYINEISN
                     DPKSKIHLTGYQARDTNGRKALESGYIENGKRTIRLNCQLELYDFSAHCGDAQLKDVV
                     KTFCDRGTETVILVHGENTQGFAQWVQEALGVKSFAPENKETIEI"
BASE COUNT         1361 a          901 c          961 g         1480 t
ORIGIN      
        1 gtcgttgagc ctggcgcaag gtcgatcgaa actgcgggaa ggatagcaga actggggcgc
       61 cagatcggga tcagcaaatt cggcgtggta attaataagg ccgcagagga agttctggag
      121 atcgaaaata agcttaaggg gtatgattta cctgtaattg gctcgatacc cttcgatcct
      181 gacatggtca aggcagacct tgataacatt tcaccgatcg agagaggggg ggcggcaatt
      241 tcttcgataa taaagatttt tgaaagatta tctaataaaa tggagtgaag agcataaaaa
      301 aacctgaacg gatacccagc gggtgcatat cgcttgacga aatgctgggc ggcggatttg
      361 aaaccgggat tgtcacgcaa atatatgggg gatcgggaac aggaaagacc aatatctgca
      421 tacagcttac agtggaatgc gtaaatagtg ggaaaaaagt gattttcata gacacggaag
      481 gtttctcagg ggagaggttc aaacagatag cgggcgagga cgcaaaaaaa attgcgggag
      541 aaataataat ctatgaacct gccagctttg agcagcagta ttcggcaatt actgatattg
      601 agaagcttat gaatgagaaa atcggtttga taattcttga ctctgcggcg cttttttaca
      661 ggctgggtct cacgcaggac gaaacaggtg aagagaacgt ggcaacgagg cgtgaacttg
      721 tcaaccagat cgggatcctt cacgggattg caagaaaata tggggtagca gtagtgatca
      781 caaaccaggt ttataaagat gttgctacag gtgaactatg cccggtggga gggaatgcat
      841 ttgagcacct ctcaaagacg attatattat tagataagac cgggccttca aaaagaagag
      901 caacattgcg aaaacacagg tcaaaaggcg aaggcgcttt ctgtgaattt atgctgattg
      961 ataaaggcgc taaatagtca aaaaaaaatt aagggataga atgcagtgat tttccggaaa
     1021 atggagtggt caaaatcatc tttacaatat ccctgcgatc ttccttgaga gaataacaac
     1081 gatgctctct cttcaaataa atacgaagga taccgagcct tgtatgcatt tgcccaacca
     1141 tggcggagac accccggtaa ttaacttcaa aaccctgctt cttaaggtaa tcataaactt
     1201 cacaggtggt ataattcctt gtctgcagaa acaggtttag aagacatttc cttatgccta
     1261 tcttatccct tacgagaaat ttcaataacc tggttttcac gccttcttca tcgttatttg
     1321 ttataaaatc acctggaata gttatgtacg aattgtttat taagcttgtt ggtttaattc
     1381 cggaaaatac atgattatgg ttttaatttt attttttcac ctttgagtat tttatagagc
     1441 ttttgtgaaa aatcatttac tttgaacatc aatgtttttg gcttgtcccc ggcaacaggc
     1501 gcagcttctt taatcgctgg gggtgcacct tctttaatcg ctgatggctc agcttcttta
     1561 atcactggtg cgggttcagg ttctcttaaa agataccatg acaggacttc ggcatatgag
     1621 atgataccga taaaagcaga ccatacaaga agcaggatgt gaagattaaa tatctgtatc
     1681 cctgaatacg agaattgaat tattgaaaag aatcccgtag cttcatccgg aaggatgaaa
     1741 accagaaaca aaggaataag tatcatgatt agagctgaca acaaaggcga tacatatacc
     1801 agaattccaa gaataatcag tgagtatata aaaaaaagca atattgaaaa aatttccatt
     1861 atttcgacct cgttctattt tttgttatta aataagctgt aaatgttatg cttccgatta
     1921 ttccggagat aacaagaaat gacgacatca taaataccat tatttcgtac ccgcttgcat
     1981 ttatatgtgt tgtatcgatt ttctttgttc cctggatgtt gttgttttct atagcgtttg
     2041 attgcgcctg cctgacagtc ccccggatat cacgatacgt tgcattcgcg ggggggagag
     2101 aaacaggatt tcctttcaat gaataccgca aatttgcgct ctttccggaa tctatataac
     2161 cagaccatgt tggtgagccg ctttccaata gaaagccgtc gggtatttta tcatcgacct
     2221 tcacaatggc cggcctgtca ccaacatttt ttatttccag ttccacatca gtcatgcttt
     2281 cgttgttcaa agatgtcttg actatgctta aatagggcat atgaacggta gtttttactt
     2341 tctttgatgt acttatctct ccattccatt tggctatggc gctggcaagt tgatatatcc
     2401 cgggtttttg gggggatatg aaatatgata catttgtaga ttttttgggt cccagttcga
     2461 tattccaatc cttttgagat gtgttttttc catcggttat atcatcatgg atatatacga
     2521 aggttttctt tgatccggaa ttatatatgg acaattcaac cagaaccgtt tcatcgtcag
     2581 tttcttcctg gacgcgtttt ataattgata tattcggagc aacggatact tccttgctta
     2641 cattggcaaa gtagacatta ttatgataat cgcgcaattc tatttttcca ttaatagtaa
     2701 gagttgaatt gggaggcagt tcagctattt gagaatgaaa ggatcttgtg ccatcgagat
     2761 cgattgcagt aaaaacatcc ggctggaatt caagggaatt gctgccatca agacttatgc
     2821 tggcaatgtt cagaagttca tcacctgtaa tgtttattga tacggaaatt ttttcatcgg
     2881 gaaaatattc ctctttgtct gtgttgatat tggcttcgaa agttgggatc ctgaatttga
     2941 ataattcaat atctataaga cccgttctat ttatatcctt tacaactatt cgcatattat
     3001 cgtaattttt gaaaccatta attgtgaaaa aatcggtcgt aacggtatta ttccttgata
     3061 cggtcagatt tactgaatct ttaccaagag attcaattcc tattgaaaca tcttcaaatg
     3121 aataggtttc tccccatttt aaggtttttt gtcctgaaga tatccagata tccctatctt
     3181 ccagtttact gatggcgatc catgaatact ccccatttat ccccaaaagg gttataccaa
     3241 ctgcatcata ttttttaaat tcatttatat taaagtcctt cttttccaat tgcaaattgt
     3301 ctttgtaaac aattattgaa gatttcgtat tatccagagc tttagctttc acgagataat
     3361 tttcaaatac tatggattga tttgatttaa taataccgga gaattttgcg ctccagactt
     3421 tttcggttgc accaaacgat gctaaaggaa aaaaacatat tattaatatg aaacatatta
     3481 taatttttat gttcatcata ctataaattg aattcaattg atttaaactt ttctgatttt
     3541 caaggattaa aatatatata tttatatctc gatcgtttcc ttattttcag gagcaaaaga
     3601 tttcacgcca agagcttcct gcacccattg agcaaaaccc tgcgtatttt caccatgtac
     3661 caggataacg gtttcagtgc cccggtcaca gaaagtctta acaacatctt ttagttgagc
     3721 atccccgcaa tgagctgaaa aatcatatag ctcaagctgg caatttagcc tgatagtacg
     3781 ttttccgttc tcgatatacc cgctttcaag cgcctttctc ccattcgtat ccctggcctg
     3841 gtaaccggta agatggatct tggatttggg atcattggaa atttcattta tataatataa
     3901 gacggggccg ccgtttaaca ttcccgcgct tgttactatt attgaaggtt cttcgattat
     3961 agctgccctg tcctgccgtt caattatatt tgcgctagtg aatgcatttt caagtttctt
     4021 tatatctggt acatattcag gcgaccgttt gatcaggtta aagacatcta cccccatacc
     4081 atcaacataa gcacgaagcc cgtgtttttt cagtattaac atgatctcct gcgtccttcc
     4141 tatgccaaat gccgggatta ttgctcttcc tccgctttca attgtttccc ttatggatcc
     4201 gatgaaccgt tcctcaagta tttttctgtc cgtatggtgc ttcccgaaat atgtgctttc
     4261 aataagtaga atatcgctat ctggcaggtc agtatcagcg ccattctgga gttcggtatt
     4321 gatagtgttc atgtccccgg tataaaaaat ggattgatca tccttttcaa gatgcaccat
     4381 tgaagaaccc ggaatatgcc ctgcatcgta aaaacatacg gaataaccat tggtattgaa
     4441 ttctttcctg tattctgttg acctggcctt tctttcgaat tcctggatat cttcattata
     4501 ataagggata ttgtggcctt ttttacgtgc gattttcagt gtatcccttg ccagcagcgc
     4561 tgacaatttt gacgtcaggg atgtgcaata tacctcaagt ccaaggtcca tgagattcgg
     4621 agccagcccg caatggtcaa ggtgtccatg ggaaatgatc atggtttttg gacgaagacc
     4681 atttgaaggg taagacggcg gat
//
LOCUS       DATGBN010000241         5801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_252071, whole genome shotgun sequence.
ACCESSION   DATGBN010000241 DATGBN010000000
VERSION     DATGBN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252071"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VKL21_05780"
     CDS             <1..342
                     /locus_tag="VKL21_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45313.1"
                     /translation="CEGSMCKVMVSRKQEQEKIAFHKLGEIMEAQNKELHKKIDTPLE
                     RAPQPPLPVIEYTNIYVLKKIPDFVGFDEKVYSLKEGSTVPIPTVNANALIKKGAVDL
                     REPVPAQVPRN"
     gene            complement(529..1257)
                     /locus_tag="VKL21_05785"
     CDS             complement(529..1257)
                     /locus_tag="VKL21_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45314.1"
                     /translation="MKTKNYIVLCDGSGNGGIAATLSIDGSLVDMMLDKIPQRIPEDF
                     TKHSRLSNELRAEIYALALGVQLISKNVIEGHIHPLAIVFLHTDSDAVMRYLYAPYEI
                     KSEHAEMIIKKIIAEKSRLLKIGIDIDISRKQHIQPTSFQSCSVHDLAAEASRGENTE
                     TIKQKIIDEHINFLNRFRYPVFDIKHLTDSIHCDMTISESLFFDMIDSGISYAAAGKL
                     TGRDPETVRTTVTRAKKKVAFPKD"
     gene            1651..2508
                     /locus_tag="VKL21_05790"
     CDS             1651..2508
                     /locus_tag="VKL21_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HMB45315.1"
                     /translation="MSYTRDLQIAAIIIVLTILFVLIPPLNNSPIRTILGIMMVLFLP
                     GYALIAALFPRKNDLDSIERLALSFGLSIAVVPLIGLGLNFTSFGLRLVPILISLSVF
                     TLAMLMIAHYRRSKLSEDERFEVPFSHIYSPIKNEIFNSKQGIDKILSIILIISILVS
                     VITLIYVIVTPKQGEKFTEFYILGDNGKAQGYPTLMEAGKNSSVIVGIANHEYMLTNY
                     TLGISLENETIKKMHVKLKHNSTWEKKVFFTPEKTGKKLKLEFMLYKEDNFTAPYRDL
                     HLWVNVSST"
     gene            complement(2456..3637)
                     /locus_tag="VKL21_05795"
     CDS             complement(2456..3637)
                     /locus_tag="VKL21_05795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMB45316.1"
                     /translation="MKILQIYDLDPIHRIGGIEVAIYELSRHLALLGHDVTVVSGADG
                     VVGEEILEGVRFINIDRSGIVRATRSLPGLSLARQAVFLPVLISMQKEKYDIYHGHVY
                     TSGIAANILARMNHGIAINTIHGSYYPAWNQLADPLTAAFYRMGEKTLAPYLAQISDL
                     QIHACHYFAEQVIKWGAQPDKIRVILNGVDTNRFDPVKIRIEKKIPFPIILTARRLVK
                     KNGVDSLVRAMPYILDQIECKLLIIGDGPEKSGLIHLVEKLELKNNVNFLGAIPHDNI
                     ARYIALADITVVPSIVEASSIFMLESMAMGKPVVATGAWGLAEIINGKNGVLTDAMHL
                     GEAIAVLLCNGQMQRELGENARQYVIENHNWENIAKRVESEYIRLMRHSPRDEDPCKV
                     P"
     gene            complement(3609..4412)
                     /locus_tag="VKL21_05800"
     CDS             complement(3609..4412)
                     /locus_tag="VKL21_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMB45317.1"
                     /translation="MNARNLPALAGERFNMGLRVDVDSIADALAVPELLLLLEKYDSK
                     ATFFITTGPDQTLMNVSHYAGKNLLNIPLKRYMPGFFHSIIRRNVESHPSLGSLMNSG
                     HEVGLHGYRHYEWMNFLHKKNMVEISNMIETGCKLFEKEFGFPPRSFSSPGFATSEEY
                     LFALDNFGFDYSSDFYGFHPFYPQVGDKTFDTVQLPVRIPSPCELQGNEEKLLGTIKE
                     FCEKDHFILYIHPSCELLYRRELLKKILGSAGKTLTLREIYENTADIRS"
     gene            complement(4409..>5801)
                     /locus_tag="VKL21_05805"
     CDS             complement(4409..>5801)
                     /locus_tag="VKL21_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMB45318.1"
                     /translation="EALERLGLIQQSKEYQEIQKLPKTINGKQNPERTKAFNELNRKY
                     GFTDYDIQHYATGIRNSWMEEHIHAHIAQKLATRAFKAVQKKAFGMAKNVRFKGKNQM
                     DSLEGKNNETGLRFKDNTLYWSDLEIPCIIDSDNDLIQYGLTHKVKYCRIVRRKINGK
                     NRFYLQLILEGTSYQNPKNKHGTEEIGLDVGTGTIATVADTKATLKQFCNELIPDQKK
                     KRKLQRKLDRSLRATNPDNYNPNGTVKKGKKFKKSNRYKAVSQDLAETDRILAAHRKS
                     LHGKDINEIIAMGTRIKTENVSYKSWQKRYGRSIGMRAPSMFMNSLKIKAENAGGYLF
                     EFPTQTTKLSQTCHICEQQVKKRLSKRWHICCGIQMQRDLYSAFLAKCVEQETSALDL
                     VRAKQLWKGMESILSDAISRAKQTAIGKVCPASFGFKGIQSQSGSLVNSVRIISEAMD
                     AVILPDESHRELI"
BASE COUNT         1596 a         1203 c         1164 g         1838 t
ORIGIN      
        1 tgtgaaggat cgatgtgcaa agtaatggtg agtaggaaac aggaacagga aaaaatagca
       61 ttccataaac tcggtgaaat aatggaggca caaaataaag aactccacaa gaagatagac
      121 acaccattag aacgggcacc acagcccccg ctgccagtaa ttgaatatac caatatctat
      181 gttctaaaaa aaattccaga ttttgtaggt ttcgatgaaa aagtatattc actcaaggaa
      241 ggcagcacag tccctatccc cacagtcaac gcaaatgctc taatcaaaaa aggtgctgtt
      301 gatttacgtg aaccagttcc ggcacaggtt cccagaaact aataaaagtt tcattatttc
      361 ttcaaaaaat gattcctgta ataccaacca agaaacccac gcactccaac gggcacagcg
      421 taggattcca cccagaccca tggatgaaga ataaagctcc attctgacag gatgtgcggt
      481 tcgtgccatg atggaatagg cagtgatgtc taccaacacc aataagacct aatctttcgg
      541 gaatgcaacc tttttctttg cgcgtgtaac tgttgttctt acagtttcag ggtcacggcc
      601 tgttagcttt cctgcggctg cgtaagaaat gccagaatcg atcatatcaa aaaaaaggct
      661 ttcggaaatt gtcatatcac aatggatcga atctgtaagg tgtttgatat cgaagactgg
      721 atatctaaat ctattaagga aattaatatg ctcatctatt attttttgtt tgattgtttc
      781 tgtattttcc ccccgacttg cttctgcggc aaggtcgtgg acactacaac tttggaagga
      841 tgtgggttga atgtgttgtt ttcggcttat atcaatatca atccctattt ttaacaggcg
      901 gctcttttca gcgatgattt ttttgatgat catttccgca tgttctgact ttatttcgta
      961 aggtgcataa aggtatctca tcacggcatc actatcagta tgtaagaaaa caatcgcaag
     1021 gggatggata tggccttcta taacattttt ggatatgagt tgaactccaa gagcaagcgc
     1081 gtagatttca gctctaagct catttgacag gcgactgtgt ttagtgaaat cttccggaat
     1141 tcgttgggga atcttatcaa gcatcatatc gaccagtgaa ccatcaatac tcaatgtggc
     1201 agcaataccg ccgtttccag aaccatcgca gagtacaatg taattttttg ttttcatttt
     1261 ttcagcctga caatgtaagg aaatgaaaca atataaacgt tacattgtca ggaaattttt
     1321 tgggtgcacc ctacaatgta aggggaataa gccgtcatac atcatatcaa tagagaatgg
     1381 atactttaat atcccatctg aacatctaga aatactttat attacaatct attagtgatt
     1441 ttcttgcgga tttgaagaca ggtgcagaat ccttgccggg ctactatccc cagttttata
     1501 gtctgttcat ttttacaaaa ccagtggaac caagcaactt acgcacttaa agatgaatta
     1561 ttttaacgac atcataatta tgtataaatt gtgtcagttc agtatatccg gaacagaaag
     1621 ctaaattagt ttgaaattat attaacgtat atgtcttata ccagagacct tcaaattgct
     1681 gcgattatta ttgttttgac aatattattc gtattgatcc ctcccctcaa taacagccct
     1741 atcagaacaa ttctgggtat tatgatggta ttattcctac ccggttatgc actgatcgca
     1801 gccctatttc ccaggaaaaa tgatctggat agcatagaaa gacttgcatt gagttttggg
     1861 ttgagcattg cagttgttcc attgatcggt cttggattga actttacatc atttggacta
     1921 agacttgtcc cgatccttat atccctttca gttttcactc tggcaatgct aatgatagcg
     1981 cattacagaa gatcaaagct atctgaggat gagagatttg aggttccatt ctcccacata
     2041 tattctccaa taaaaaatga aatattcaat tcaaaacaag gaatagataa gattctctcg
     2101 ataattttga ttatatcaat attggtttcg gtaattactt taatatatgt aattgtaaca
     2161 ccaaaacagg gtgagaaatt cactgaattc tatatcctgg gagataatgg gaaggcacag
     2221 ggctatccga ccttgatgga agctggaaag aatagtagcg taatagttgg catagcaaat
     2281 cacgaatata tgctgacaaa ctatacgctt ggcatatcac ttgaaaacga aactattaaa
     2341 aaaatgcatg tcaaacttaa gcataattct acatgggaga aaaaagtatt tttcactcct
     2401 gaaaaaaccg gaaaaaaact caaacttgaa ttcatgcttt ataaagagga taattttacg
     2461 gcaccttaca gggatcttca tctctgggtg aatgtctcat caacctgatg tactctgact
     2521 ctacacgctt tgcgatattt tcccagttat ggttttcaat cacgtattgg cgcgcgtttt
     2581 ccccgagttc cctttgcatt tgcccattgc aaagcaatac tgctatcgct tctccaaggt
     2641 gcatagcatc tgtcagcacc ccgttctttc catttatgat ctctgcaagt ccccatgcgc
     2701 ctgtggcgac cacgggttta cccattgcca tggattcaag cataaagatg cttgatgctt
     2761 ctacaatgct tgggaccaca gtgatgtctg caagcgctat gtatcttgca atattatcat
     2821 gggggatagc gcccaggaaa ttcacattat tttttagttc caatttttca acaagatgaa
     2881 tgagaccgga tttttcggga ccatctccta taataagaag tttgcattca atttgatcca
     2941 gtatataggg catggctcgc accagactat ccacaccatt tttttttaca agccttctcg
     3001 ctgtgagaat gatggggaat gggatttttt tttctattct gatctttacc ggatcaaatc
     3061 tattggtatc aacaccgttc aagatcaccc tgatcttatc gggttgggcg ccccatttaa
     3121 ttacctgctc tgcaaaataa tgacaggcat gtatttgaag atcggaaatt tgtgcaagat
     3181 atggggcaag agtcttctcg cccatcctgt agaatgcggc ggttaaggga tctgcaagct
     3241 ggttccatgc aggataatat gatccatgta tggtgttaat ggcaattcca tgattcattc
     3301 ttgcaaggat atttgcggct attccacttg tgtagacgtg tccatgataa atgtcatatt
     3361 tctctttttg catggaaata aggactggaa gaaaaactgc ctgcctggca agggacaatc
     3421 cgggaagtga tcgcgtcgca cgcacgatac cagagcgatc gatgtttatg aacctgacgc
     3481 cttccaggat ctcttcgcca accacaccgt cagcgcctga aactactgtt acatcatgtc
     3541 ccagcagggc aagatggcgg gagagttcat atatcgctac ctctatacca ccaatgcgat
     3601 ggatggggtc aagatcgtat atctgcagta ttttcatata tctccctgag cgttagcgtt
     3661 tttcccgcgc tccccaggat tttcttcaga agttctctcc tgtagaggag ttcgcaggat
     3721 ggatggatgt ataatatgaa atgatctttc tcgcagaatt cctttattgt tccaagtaat
     3781 ttttcttcgt taccttgaag ttcgcaaggt gatggaatcc ttaccggcag ctgtaccgta
     3841 tcgaatgtct tatccccgac ttgcgggtaa aaaggatgaa aaccatagaa gtcacttgaa
     3901 taatcgaacc cgaaattatc aagcgcaaaa agatattctt cgcttgttgc gaagccgggc
     3961 gaagaaaaag aacggggggg aaaaccaaat tctttttcga acaatttaca tccggtttcg
     4021 atcatattgg agatctccac catatttttt ttatgaagga aattcatcca ttcgtaatgc
     4081 ctgtatccat gaagcccgac ctcatgacct gaattcatca aagatccaag agaaggatgt
     4141 gattccacat tgcggcggat aatgctatgg aaaaatcccg gcatgtatct tttcagcgga
     4201 atatttagca ggttttttcc cgcatagtga gaaacattca tcaatgtctg gtcaggacct
     4261 gtggttatga aaaatgtagc ttttgaatca tatttctcaa ggagaagaag aagttcaggt
     4321 actgccaggg catcagcgat gctgtcaaca tcaactctta gccccatgtt gaacctctcc
     4381 ccggctaaag ccgggagatt ccgcgcgttc atataagctc tctatggctc tcatccggga
     4441 ggattacagc atccatagcc tcacttataa ttcgtacaga attaacgagc gaaccgctct
     4501 ggctctggat tcccttgaat ccaaagctgg caggacaaac cttacctatc gcagtttgtt
     4561 tcgcccttga aatcgcatcg ctaaggatag attccatacc tttccagagt tgtttcgctc
     4621 tgactaaatc aagggcgctt gtttcttgtt ccacacattt tgccagaaat gcagaataca
     4681 agtccctctg catctggata ccacaacaaa tatgccatct cttgctaaga cgtttcttta
     4741 cctgctgttc gcagatatgg catgtttgag atagtttcgt tgtctgtgtt ggaaattcaa
     4801 ataagtagcc accagcattt tcagctttaa ttttgaggct attcatgaac atggatgggg
     4861 cacgcatccc gattgacctg ccgtatcgct tttgccagct tttataagat acgttttcag
     4921 tcttgattcg tgtgcccatt gctattattt cgtttatgtc ttttccatga agggattttc
     4981 gatgtgcagc taagattcta tcggtttcgg ctaagtcttg gcttactgct ttatatcgat
     5041 ttgatttctt gaacttcttc cccttcttga ctgttccgtt tggattataa ttatcaggat
     5101 tggttgccct taatgaccta tcgagttttc gttgcagttt tcgtttcttt ttctgatccg
     5161 ggattaactc attgcaaaac tgtttcaggg tagcttttgt atcggctact gttgctattg
     5221 tacctgttcc tacatccagt ccgatctctt ctgtcccatg cttgtttttt gggttctggt
     5281 atgatgttcc ttcaagaatt aattgaagat agaatctgtt cttgccattg atctttcttc
     5341 gcacgatccg gcagtatttg actttgtggg ttaagccgta ttggattaaa tcgttatcag
     5401 agtcgattat gcatggtatt tcaaggtctg accagtagag ggtattatct ttgaatctta
     5461 atccagtttc gttgtttttt ccttccagag aatccatctg gttttttcct ttaaatctta
     5521 cattctttgc cattccaaat gcttttttct ggacggcttt gaaggctctt gttgctaatt
     5581 tctgggcgat atgagcgtga atgtgttctt ccatccagct attccgtatt ccagttgcat
     5641 aatgctgaat atcataatcg gtgaacccat atttcctgtt gagttcgttg aatgcttttg
     5701 ttcgttctgg attctgttta ccgttaatgg ttttcgggag tttctggatt tcctgatact
     5761 cttttgattg ttggataagt ccaagtcgct ccaaggcttc c
//
LOCUS       DATGBN010000242         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_18823, whole genome shotgun sequence.
ACCESSION   DATGBN010000242 DATGBN010000000
VERSION     DATGBN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18823"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VKL21_05810"
     CDS             <1..453
                     /locus_tag="VKL21_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45319.1"
                     /translation="VLSDVDFYVSKAKETAVKLNKEISGELERLKPAYGKGISEMLKE
                     AERYASERQIYGMHVYIENAINYAAKAGIDISGKLPKFMHSYEEGLKEALQEAEKHEA
                     EGFLGMSYQYIHRADNYAAKLEKNIENILKSLPWYEKWTLTDIHMKLR"
     gene            460..627
                     /locus_tag="VKL21_05815"
     CDS             460..627
                     /locus_tag="VKL21_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45320.1"
                     /translation="MCMVGDVPEFNMENIEMDLKEYICLDCENKFKGLGKSVICPACK
                     SSNIEMVSAEK"
     gene            624..911
                     /locus_tag="VKL21_05820"
     CDS             624..911
                     /locus_tag="VKL21_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45321.1"
                     /translation="MKSCCCEISDAAAYYQGETKSGEKWTPAFIKYVDKEKCNGCGLC
                     VKVCSRGVYEIQELNGRKISVPINASNCVGDGSCHMVCKQNAMVCVPRRIK"
     gene            complement(926..>2036)
                     /locus_tag="VKL21_05825"
     CDS             complement(926..>2036)
                     /locus_tag="VKL21_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45322.1"
                     /translation="QAFYKKVDDIFCLKRKFPARIRNLSLAAMLFLVITWADFQFNLV
                     NSPLLTAYFIVALLGLVVIISIIFERRYFCRYACPITGLIGLYSMFAPVELRAKDKET
                     CKACTEKYCISGNEKGYPCPVFEYPGTMEKNTHCILCTECVKTCHRNNISFNLRSFAG
                     DFLNLKKTRMDESLFILMLLGVTIFQTLIMIRPWAVITHDLMTYTGASYDTVRFVLFI
                     TSAASPILIYSIVISVSKLLNPKTSFKDLFAGYAYSIIPMGLLMHLSHNLRHLLEEGT
                     GIIPVISDPFGFGWDLFGTSGYMPAPLLYNNTILLIQWLLMFIGLGYSISIGKKISRR
                     KFRGNNSAYFPVLVYVLLFFVFNLWILGQPIMHKH"
BASE COUNT          692 a          348 c          457 g          539 t
ORIGIN      
        1 gttttgtccg atgttgattt ttatgtatca aaggcaaagg agactgccgt aaaactcaac
       61 aaggaaatca gcggcgaact tgaaaggcta aagccagcat atggaaaagg aatctctgaa
      121 atgcttaaag aagcagaaag atatgcatca gaaagacaaa tctatggaat gcatgtgtat
      181 atagaaaatg caataaatta tgcagctaag gcaggtatcg atatttcagg gaagcttccg
      241 aaatttatgc attcatacga agaaggtctc aaagaggctt tacaggaagc agagaagcat
      301 gaagctgaag gtttccttgg catgtcgtat caatatattc atagagcgga taattatgct
      361 gcaaagcttg aaaaaaatat agagaatatc ttaaaatcat taccgtggta tgagaaatgg
      421 acactgacag atattcacat gaaattgagg tgaaaaaata tgtgcatggt cggagatgtg
      481 cctgaattca atatggaaaa catagaaatg gacttaaaag aatacatttg cctggattgt
      541 gaaaacaaat ttaaaggact tggaaaaagc gttatctgtc ctgcctgcaa atccagcaac
      601 attgaaatgg tgagtgcaga gaaatgaaga gttgctgttg tgaaatcagc gatgctgctg
      661 catattatca aggagaaaca aaatcaggcg agaaatggac tccagccttt attaaatacg
      721 ttgataagga aaaatgcaat ggctgtgggt tgtgcgtgaa ggtctgctca aggggagttt
      781 acgagatcca ggaattaaat ggcagaaaga tttcagttcc aataaatgct agcaactgcg
      841 ttggagatgg gagctgccac atggtttgca aacagaatgc aatggtttgc gtaccaagga
      901 gaataaaata attggcattt gaagtttagt gtttatgcat tatcggctgc cccagtatcc
      961 atagattgaa cacgaaaaat aaaagaacat atactagtac tggaaaatat gctgaattat
     1021 ttccgcgaaa cttccgtctt gaaatctttt tgccgattga aattgagtac cccaatccaa
     1081 tgaacataag cagccactgg atcaagagga tggtgttatt gtagagaagc ggtgcgggca
     1141 tatatcctga agtcccgaat aaatcccatc caaaaccaaa aggatccgaa attacaggaa
     1201 tgattccggt cccttcttcc agtaaatggc gcagattatg ggatagatgc atcagcaacc
     1261 ccattggaat gatagagtac gcatagccgg cgaataggtc tttaaatgat gtttttggat
     1321 tgagcaattt tgaaacagag atgactattg aataaatcag gattggagat gccgccgaag
     1381 taatgaataa aacgaatcga accgtatcgt aactggcgcc ggtataggtc attaaatcat
     1441 gtgtaataac agcccacggc cgtatcatga tcaatgtctg gaatatagtc actcccagga
     1501 gcatcaatat gaatagtgat tcatccattc gggtcttttt caaattcaaa aaatctcctg
     1561 caaaggaacg aagattgaaa gagatgttgt tccggtggca ggtttttacg cattctgtac
     1621 agagaataca atgagtgttc ttctccatgg ttcccggata ctcgaacacc gggcagggat
     1681 aacctttttc gttgcctgaa atacaatatt tttccgtgca ggctttgcag gtttctttat
     1741 cttttgctct taattcaaca ggtgcaaaca ttgaatataa tcctattaat ccagtaatgg
     1801 ggcatgcgta cctgcaaaag taacggcgct cgaatattat tgatattatt acaacaagac
     1861 cgagtaaagc aacaataaaa tatgcagtca aaagcgggct gttcacaagg ttgaactgga
     1921 aatcagccca tgtgatcaca agaaatagca tcgctgccag ggaaagattt ctgattcttg
     1981 cgggaaattt cctctttaga cagaatatgt catcaacttt tttataaaat gcctgg
//
LOCUS       DATGBN010000243         1975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_213657, whole genome shotgun sequence.
ACCESSION   DATGBN010000243 DATGBN010000000
VERSION     DATGBN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213657"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..973
                     /locus_tag="VKL21_05830"
     CDS             341..973
                     /locus_tag="VKL21_05830"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45323.1"
                     /translation="MGRPVTNTPEVMFRSAIIQSLYDYSDREMESEARYNIMVKWFIG
                     LNLDDSSYDHSALGRFRDLLGEERWKEIFFSLLKQIENAGFVKGKEYVDATHVIANIA
                     IPGTIDLLRQGIKDVMKAIQETDPSLYEELGGEKTANDDKKVYKLSPEERESKLVELV
                     KKAREINQKSERIGGKVGEKTRQMRRILNENIEENENKIAKKKIRLKTDS"
     gene            970..1416
                     /locus_tag="VKL21_05835"
     CDS             970..1416
                     /locus_tag="VKL21_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45324.1"
                     /translation="MSIVDKDARHGAKSDERKFTGYKVNSIMSEDGFIKNINATPGNS
                     YDGNILIPLIDEMIEQGFTACEVVGDTAYGEVYNRNKLMLKGITIIAPFKKETNKNGL
                     FPQSKFSIEKHAVVCPEDAGRWYGITIRRLKKSRSFLIKRIVETVN"
     gene            1413..1694
                     /locus_tag="VKL21_05840"
     CDS             1413..1694
                     /locus_tag="VKL21_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45325.1"
                     /translation="MKQKCTVQKRRTIAISEHYELLKKAKEYNKTDDFKEDMKQRAHI
                     EPKQGEMKRYHGLQRAKYWGLPKMNIQATISAIVVNVKRLVTLNSCGYR"
BASE COUNT          765 a          287 c          402 g          521 t
ORIGIN      
        1 atgaatagta aaaaaaagtt tttatctgcg tttttaatta ttactgagag cgtcgtaaaa
       61 gtacagaact gttaaattta taatagtatg ttgcactttt cgtttctttt tttatcccaa
      121 atcaaatcac ttatatacgg ttaacacttt catcacataa aatacatact ttttgagtga
      181 taatatgttt tctcccataa aaggaaagca gaccacgttc gtttatggcc ctgagattta
      241 tgagaaactc gtatctagag atcatcttct atacaagatc aatgagtcaa tgaatttcac
      301 tttcatcaat ggagaatgtc gtaatcttta ctcagaaaat atggggagac ctgtaacgaa
      361 cactcctgaa gtcatgttta gatcagctat tattcagtct ctctatgact actcagatcg
      421 agaaatggaa tccgaagcac gctataatat aatggtaaaa tggttcattg gtttgaacct
      481 tgacgattct tcctatgatc atagtgcact tggacgtttc agggatcttc tgggggaaga
      541 aagatggaaa gaaatattct tcagcctatt aaagcaaatc gaaaatgctg gattcgttaa
      601 aggtaaagag tacgtggatg ctactcatgt aatagcaaac attgcaattc caggtacaat
      661 agacctgtta agacagggaa ttaaggatgt aatgaaagct attcaggaga cagatccaag
      721 cctatatgaa gaacttggtg gagaaaaaac tgctaacgat gacaaaaagg tatacaagtt
      781 aagccctgaa gaaagagaaa gcaagctagt tgaactggtt aaaaaagcca gagagataaa
      841 tcagaaatcg gaacgtattg gaggaaaagt aggtgaaaaa acaaggcaaa tgaggcggat
      901 tcttaatgaa aacatagaag aaaatgaaaa taaaatcgca aaaaaaaaga taagactaaa
      961 gacagactcg tgagcattgt tgacaaagat gcaagacatg gtgctaaatc agatgaaaga
     1021 aagttcacgg gatacaaagt gaactcaata atgtctgaag atggattcat aaaaaacatc
     1081 aatgcaactc caggtaattc ctatgatggg aatatactta tccctctaat tgatgaaatg
     1141 attgaacagg gatttactgc atgtgaagtg gtcggagata ctgcttatgg agaagtttac
     1201 aacaggaata aactgatgtt gaaagggata actataatag ctccctttaa aaaggaaact
     1261 aacaaaaatg ggctctttcc acaaagtaaa ttcagtatcg agaagcatgc agtagtatgt
     1321 cctgaggacg caggacgatg gtatggaatc acaataagaa gactgaaaaa atcacgttct
     1381 tttttaataa aaaggattgt ggaaactgta aattgaaaca aaagtgtact gttcagaaaa
     1441 gaagaacaat tgccatcagt gaacattatg agctactaaa aaaagcaaaa gaatataata
     1501 aaactgacga tttcaaggaa gatatgaaac aaagagctca tatcgaacca aagcaaggag
     1561 aaatgaaaag atatcatggg ttgcagaggg caaaatattg gggacttccg aaaatgaata
     1621 tacaagcaac aataagtgca attgtggtca atgtaaaaag acttgttaca ttgaatagct
     1681 gtggctatcg atgacgctca taaaaatatg taaaaagaat gaaaaattga aagaaaagga
     1741 tgaaaagaat gaatatattt gatgaaaata cctctaaaaa ttggaggatt tttatgaagt
     1801 gcaaaatttt gcgctgaaat ctttactatg ggtaaccatt agctactttt acgactctat
     1861 ctaaacaatg gcaagaaacg tgccaaactc gtgcattcct tgtgaacctg caaatttggt
     1921 cttaataatc aagctgtgga taaatattat gggagcggtc acgtgcgcga ttgga
//
LOCUS       DATGBN010000244         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_330630, whole genome shotgun sequence.
ACCESSION   DATGBN010000244 DATGBN010000000
VERSION     DATGBN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330630"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VKL21_05845"
     CDS             <1..289
                     /locus_tag="VKL21_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB45326.1"
                     /translation="LKKLKVNGKLANNSAKPEKNERDIILFANNSILMSIDEAAAIRY
                     AKVRYDLKKKGNPIPENDIWITALCLGQNVPLLSNDGHFDKIKGLEVINWQ"
     gene            complement(336..503)
                     /locus_tag="VKL21_05850"
     CDS             complement(336..503)
                     /locus_tag="VKL21_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45327.1"
                     /translation="MITLPIWIERALPIMKNGIISAGETFERWGIALKFITDRLSQDI
                     SRLLDKYVFGS"
     gene            588..734
                     /locus_tag="VKL21_05855"
     CDS             588..734
                     /locus_tag="VKL21_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45328.1"
                     /translation="METMDSASELQTNKQFLKAIKDADREFKEGKTISFDELVEELEL
                     KNEF"
     gene            complement(796..1215)
                     /locus_tag="VKL21_05860"
     CDS             complement(796..1215)
                     /locus_tag="VKL21_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB45329.1"
                     /translation="MNRILIDTMYIEEIFSCEEALYTNFADAIDDGKIIGISSVVSLT
                     ELVKNLGMKDMNRMHTTVRELKSSPIILINVDQKIAERAGELRLRYDIPTVDSMIAAT
                     GIVEGISHVLTRDILHFGSIKNLIKIIDLKTALKLAR"
     gene            complement(1212..1481)
                     /locus_tag="VKL21_05865"
     CDS             complement(1212..1481)
                     /locus_tag="VKL21_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMB45330.1"
                     /translation="MQQIRMSRKGLVVIPVNIRKKLNLQYGETLFIEEDEGTIKIKPK
                     VKLRSLCGTWPELNIETITKEILEDRELDEKADKEREKLIGRRIK"
     gene            1542..>1824
                     /locus_tag="VKL21_05870"
     CDS             1542..>1824
                     /locus_tag="VKL21_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45331.1"
                     /translation="MVKNTHPDKYEEFKKNFDEIKKTAVIKESTTRTEVTGPEPPKIV
                     EKEKPPEPPKEPPKEPEKKKEGDGASKHILIRQPAELMLSSSMGRALSIQ"
BASE COUNT          583 a          355 c          320 g          566 t
ORIGIN      
        1 tttgaaaaaa ttgaaggtca atggtaagct ggcaaacaat agcgcaaaac ctgaaaaaaa
       61 tgagcgtgac ataatcctgt ttgccaataa ttctattcta atgtcaattg atgaagctgc
      121 tgcaatacgc tatgctaaag tacgatatga tctgaaaaag aaaggaaatc ctattcctga
      181 aaatgacata tggataacag cattatgttt aggacagaat gttccgctac tgagcaatga
      241 tggtcatttt gataaaatca aaggattaga agtcattaac tggcaatgaa tcccttttgt
      301 gcccctttga acccatgctg taaaatattg tttattcaac ttccaaacac atacttatcc
      361 agcagcctgc tgatatcctg cgaaagcctg tcggtaatga acttaagggc aatcccccat
      421 ctttcaaaag tttcacctgc tgaaataata ccatttttca ttattggaag cgccctctct
      481 atccagatgg ggagtgtgat cactaataat gcaagaatga gtaatatgaa gataaaaaag
      541 atttttttgt acgaaaagct aaatagtact gaagacatat caaaattatg gaaaccatgg
      601 attccgcttc tgaactccaa accaacaagc aatttctaaa ggctatcaag gatgcagacc
      661 gagagttcaa agaaggcaaa accatatcct ttgatgaatt ggtagaggaa cttgagttaa
      721 agaatgaatt ttgaagttgt atacatcttt atagcttcaa aaacataaaa aggaagctgt
      781 aatatccttc ttttcttacc tggcgagttt taaagccgtt ttgaggtcaa ttattttaat
      841 cagattttta atagacccga aatgaagtat atctcttgta agaacatgac tgataccttc
      901 gacaataccg gttgccgcaa tcattgaatc aacggtgggg atatcatatc tcaatcttaa
      961 ttcaccagca cgttctgcaa ttttttgatc cacatttatg agaattattg gtgaggattt
     1021 taattcccgg acagttgtat gcattctatt catatccttc atgcccagat tttttaccaa
     1081 ttccgtaagg gaaacgactg aagatattcc aattattttc ccatcatcta tagcatctgc
     1141 aaaattggta taaagtgcct cttcgcaact gaatatttct tcgatataca tagtatctat
     1201 tagaatccga ttcatttaat cctccttcct attagtttct ccctctcttt atcggctttt
     1261 tcatcaagct ctctatcttc gagtatttct tttgttatgg tttcgatatt aagttctggc
     1321 caggtgccgc agagggatct cagtttaacc ttgggtttta ttttaattgt gccttcatct
     1381 tcctctataa acaatgtctc accatattga agatttaatt tttttctgat atttacaggt
     1441 atgactacca atccctttct gctcattctt atttgttgca ttctttcacc tttatatcta
     1501 actatgttta actatgttaa acttagtggg atattaatat tttggtcaaa aacacgcatc
     1561 ctgacaagta tgaggaattc aagaagaact ttgacgaaat caagaaaacc gctgttatta
     1621 aagagagcac aaccaggaca gaagtcacgg gcccggagcc tccaaagata gtggaaaagg
     1681 aaaaacctcc ggagccgccc aaagaacctc caaaggagcc agagaagaaa aaggaaggtg
     1741 atggtgcttc caaacacata cttatccggc agcctgctga attgatgctc tcttccagta
     1801 tgggaagagc cctatctatc caga
//
LOCUS       DATGBN010000245         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_58530, whole genome shotgun sequence.
ACCESSION   DATGBN010000245 DATGBN010000000
VERSION     DATGBN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58530"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VKL21_05875"
     CDS             complement(<1..416)
                     /locus_tag="VKL21_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HMB45332.1"
                     /translation="MMNTKAINRLIEPEQKGETKFIFFSGKGGVGKSTMSCATAVWLA
                     KNGYKTLLVTTDPAPNLSDIFGQQIGHKITAINNVENLFSIEINPDTASEEYKERIIA
                     PMRELLDEKNIQVIKEQLNSPCVEEVTAFDKFIEFM"
     gene            complement(419..745)
                     /locus_tag="VKL21_05880"
     CDS             complement(419..745)
                     /locus_tag="VKL21_05880"
                     /inference="COORDINATES: protein motif:HMM:NF018637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HMB45333.1"
                     /translation="MENINLVIYEGAMCCSTGVCGPEPNKELKELNEALKRLQKEFKE
                     LKAVRASISFNLNLFLENQDISQLVKENGPGVLPITTINGKIIARKKYLTYSELKKEI
                     ENTRVT"
     gene            complement(746..1039)
                     /locus_tag="VKL21_05885"
     CDS             complement(746..1039)
                     /locus_tag="VKL21_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMB45334.1"
                     /translation="MSWFNEKTPDAAKAFAELRNSVYKDGALDLRTKELISIAASVLM
                     RCERCTEIHAQRAKKLGATDEMIAEAVNCAMFVAAGSQLSWSDVYEKIMEKKE"
     gene            complement(1043..2182)
                     /gene="arsB"
                     /locus_tag="VKL21_05890"
     CDS             complement(1043..2182)
                     /gene="arsB"
                     /locus_tag="VKL21_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HMB45335.1"
                     /translation="MKERTGCVGGTNSGGLGVISKYLTLWILLAMAGGIILGAVYPQV
                     ATILDAVRIEQVSLPIAIGLIWMMYPPLAGVKYEELYKMKQQGKALSASIIQNWIIGP
                     VLMFALAWIFLPDLPEYRIGLILIGLARCIAMVLVWNQLAGGDNELCAVLVAVNSIFQ
                     VALYSFLAYLFITVLSAWIGGAGAGAVVDISIWQVAKAVFIYLGIPFIAGIITRSVLV
                     KKRGKDWYDNVFMKKLGPTALIGLLFTIIVMFSLKGEFIVKLPMDVFRIAVPLLVYFI
                     IMFGISFFMSYRLGFSYDQSTTLAFTAASNNFELAIAVSVAVFGIGSGVAFAAVVGPL
                     IEVPVMLGLVHVARRASVMWFDQNGLPINRRITANVTGNTNKKEE"
     gene            complement(2304..2678)
                     /locus_tag="VKL21_05895"
     CDS             complement(2304..2678)
                     /locus_tag="VKL21_05895"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMB45336.1"
                     /translation="MNDQCQCESKTAELCCPGDENIRKEWLMGLEKEHNPVRHTSKEI
                     VKKLKLLSNSHRIEILLMLEQREHCMDEIARKLKAKKSAVSYHLGLLKKHGMICKIKH
                     SRFAFYSLSQEGIRKIIIFRKL"
BASE COUNT          724 a          621 c          505 g          851 t
ORIGIN      
        1 tccataaatt ctatgaattt gtcaaatgct gtaacttcct caacgcaggg actgttcagt
       61 tgttctttta ttacctgtat gttcttttca tcaaggagtt ctctcatggg ggcaatgatt
      121 ctttctttat actcctcgga agcagtatct gggtttatct cgattgaaaa caggttttca
      181 acattgttaa ttgcagtgat tttatgtcct atctgttgac caaatatatc cgaaagattt
      241 ggagccgggt ctgtggtaac gagcaatgtt ttataaccat ttttcgcaag ccacacagca
      301 gtggcacagc tcattgtgct tttcccaact ccgccctttc cgctgaaaaa tatgaatttt
      361 gtctctcctt tttgttctgg ctctattaat cggtttatgg ctttagtatt catcattatc
      421 aagttactct ggtattttcg atctcttttt tcagttcaga gtatgtcaga tatttttttc
      481 tggcaattat cttgccgttg atagtggtaa tggggagtac gcctggtcca ttttccttta
      541 ccagctgtga gatatcctga ttctcaagga aaaggttcag gttgaaagat atgcttgccc
      601 ttactgcttt caattctttg aattcttttt gcaatctttt taatgcctca ttaagctctt
      661 tcagttcctt gttcggctcc ggaccgcaaa caccagtgga gcagcacatc gcaccttcat
      721 atatgaccaa atttatattt tccatctatt cctttttctc catgatcttt tcatatacat
      781 cgctccatga taactgggaa ccagcagcca caaacatggc acagtttaca gcttcagcga
      841 tcatctcatc agtcgcgcca agttttttcg cgcgctgggc atggatttcc gtgcagcgct
      901 cacacctcat aagcactgaa gcggctatag agatgagttc ttttgtgcga agatcaagcg
      961 caccatcttt atataccgaa tttctcaatt cagcgaatgc ttttgcagca tccggtgttt
     1021 tctcgttgaa ccatgacaaa ttttattcct cctttttatt cgtatttcct gtaacattag
     1081 cagtaattct tctatttatc ggcagaccat tttgatcaaa ccacatgaca ctcgctctcc
     1141 ttgcgacgtg tacaagtccc agcataacag gcacttctat aagtggccct accacagcag
     1201 caaaagctac acctgagcct atcccgaaca ctgcaaccga tactgcaatt gcaagttcaa
     1261 aattattgct tgccgcggtg aatgccaggg ttgtggattg gtcataagaa aaacccagtc
     1321 tgtacgacat gaaaaaagaa ataccgaaca ttataatgaa atagacaagc agcggaactg
     1381 ctatcctgaa tacatccatg gggagtttta caatgaactc tccctttaag gagaacatga
     1441 cgataatggt aaagagcagt ccgatcaacg cggtgggacc aagctttttc ataaatacat
     1501 tatcgtacca gtcctttcct ctctttttta caagaacaga ccgtgttatt atgcctgcga
     1561 taaatggaat tcccagatag atgaatactg ctttagctac ctgccatatt gatatatcca
     1621 caacagcgcc tgctcctgca cctcctatcc atgctgacaa tactgttatg aataaatagg
     1681 cgaggaaaga ataaagcgct acctggaaga tagagttcac agccacaagg accgcgcaca
     1741 gttcattatc cccacctgcc agctggttcc agacaagaac catggcgatg catcgcgcaa
     1801 gaccgatcag gatcaaacct atgcggtact ctggaaggtc tggaaggaat atccaggcaa
     1861 gggcgaacat gagaacagga ccgataatcc agttctggat aatggaagca ctcagggctt
     1921 ttccctgctg cttcattttg tagagttctt cgtatttcac acctgcaagc ggcgggtaca
     1981 tcatccagat aagacctatc gctattggaa gtgagacctg ttcgatcctg acagcatcaa
     2041 gaattgtggc tacctgcgga taaacagcgc caaggattat ccctccagcc atggcgagaa
     2101 ggatccagag ggtcaggtat ttgcttatta cgccaagacc gcctgaattt gttcctccaa
     2161 cgcaacctgt tctttctttc aagagatcac ctttctgttg tttgcattct tcattccaat
     2221 acctgccttc cagttatcat tctaacactt tataagtgca tacgattaat aaacccctac
     2281 aaggaatttc aaaaaaattt gaactatagt tttcgaaaaa ttataatctt tcttatccct
     2341 tcttgagaaa gtgaataaaa tgcaaaccga gagtgtttta ttttgcatat cataccgtgt
     2401 ttttttaata gccccagatg ataggataca gcagactttt tagccttaag ctttcttgcg
     2461 atctcatcca tacagtgttc tcgttgctca agcatcaaaa ggatctcaat gcgatgggaa
     2521 tttgagagaa gctttaattt cttgacaatc tcttttgagg tatgccttac aggattatgt
     2581 tctttttcca gacccataag ccattctttg cgaatgtttt catcacccgg acagcataat
     2641 tctgctgttt tggattcaca ctgacattga tcattcataa ttcatgcatt ttgtttcttt
     2701 t
//
LOCUS       DATGBN010000246         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_253150, whole genome shotgun sequence.
ACCESSION   DATGBN010000246 DATGBN010000000
VERSION     DATGBN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253150"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VKL21_05900"
     CDS             complement(<1..878)
                     /locus_tag="VKL21_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMB45337.1"
                     /translation="MARNIRVEKITQTPIEKQEIEIVERKGVGHPDSMADGFAEAVSR
                     ALCNEYIRKCGTILHHNTDQVEVVAGRSNPEYKGGELISPIYVLLVGRATKEFKDIKI
                     ATDTTAVRAAKQYLKTTLPDIDPEHDVIIDCKLGTGSTDLQSVFKRGKAPMANDTSFG
                     VGHAPFSEVEQLVYNTERNMVLNFKKVLPAIGTDIKVMGMRKNEKITLTVACAMIGKY
                     VDDQSHYFNIKEELRNKICDLALKYTDREVEVFVNTGDDEKTGCVYLTVTGTSAEMGD
                     DGSVGRGNRCNGLITP"
     gene            969..1922
                     /gene="twy1"
                     /locus_tag="VKL21_05905"
     CDS             969..1922
                     /gene="twy1"
                     /locus_tag="VKL21_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HMB45338.1"
                     /translation="MLDSEFDTMKEIASLSETTRSLLKKHGYHLAGTHSAVKTCLWLN
                     KSIRNSGACYKSKFYGMNSHRCIQMTPTLVCNHRCLHCWRAVEVPVEIPTVWDSPDRI
                     VNDVLLEHKRLISGYGGSEFMDKTRWKEAFSPKHVAISLSGEPTLYPYLPELIEEFHK
                     REMTTFVVTNGTNPEMIEKIRPTQLYISLNAPCEEIYEKACSPLGNSWENIRRSLEIL
                     KGSCTRTAIRITLTKGVNIIDPEGYARLIELAQPDYVELKAYMHLGFSRKRLSRENMP
                     SYDEVLDFSRRVAKVLDYCIADDSMGSRVVLLSKDGGKTVI"
     gene            1964..2656
                     /locus_tag="VKL21_05910"
     CDS             1964..2656
                     /locus_tag="VKL21_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HMB45339.1"
                     /translation="MTEGEAKVIPGENILEEPIEILVNLAKNGEIDPWNINIVELTDK
                     FLKRVDDLEKMDLRVSGRTLLYASVLLRMKSNALVEVEEPPEEIDIESDNFEISDYPV
                     PSMPLRRYSKRPVTLEELLTELKRAEAFEKKKLNRVENKKDEIRATLEDVLSVAHDED
                     IESRIGKMRCILNELLDKQKSIKFSEISSTLDRTGILMAYLAILFLATKKEIWIEQDE
                     FFGELFISRTAS"
     gene            2653..3747
                     /gene="scpB"
                     /locus_tag="VKL21_05915"
     CDS             2653..3747
                     /gene="scpB"
                     /locus_tag="VKL21_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMB45340.1"
                     /translation="MSGDKEILEAALFASGEPLDIGQLKNLVRGKNVREMLQELVDEY
                     VARDSAIEIKEMDGRFIMQVKPEYAQKVRSIAPKELRSPVLRTLAMIAYHQPITVAEL
                     VNRRGAATYDHVRELEESGFISALQQGRTRLLQTTPRFAEYFNLESGDPEAIKLKIIE
                     LAREQKMGLDKWLGKQGIGVSPMYESLMELCGIAEYKVINPYAPGEDEQDRIQELGVL
                     VISKGYKERVSKYFDGRIIEIQATTFEDLINSVNILAEYGIRRKTKESIEFISELRDE
                     YIEKANFITAKVMPQTEMVSRMINEMGLGISGDGVKIAPDYGTSSEGKEIGIGADILI
                     PTHKNAQMDIVNRICQRYDAVIEGLENRNK"
BASE COUNT         1240 a          781 c          894 g         1098 t
ORIGIN      
        1 ttgggagtga tcaggccgtt gcacctgttc ccgcgcccca ctgagccgtc atcacccatt
       61 tcagctgagg ttcccgtaac tgtcaggtaa acacagcctg ttttttcatc atcgcccgta
      121 ttgacgaaaa cttctacttc ccggtcagta tattttaaag caaggtcaca tattttgttt
      181 cgaagttctt cctttatatt aaaataatgt gactggtcat caacatattt gcctatcatg
      241 gcgcatgcaa ccgtgagggt tattttttcg ttcttcctca tacccattac tttgatatct
      301 gtcccgatgg ctggaagaac cttcttgaaa ttcaagacca tatttcgttc cgtattgtaa
      361 actaactgct caacttcaga aaagggagca tgacccacac caaaagaagt atcattagcc
      421 attggcgctt ttccccgctt aaaaacactc tggagatcag ttgagcctgt acccaattta
      481 cagtctatga taacatcatg ttcgggatca atgtcaggaa gtgtagtttt caggtattgc
      541 tttgcagctc ttacggccgt agtatcagtg gctattttta tatccttaaa ttctttcgtt
      601 gcccttccaa caagcaagac ataaatgggt gatatcaatt ctcccccttt atattccgga
      661 ttggagcggc cggctacgac ttctacctgg tctgtgttat gatgaaggat cgttccgcat
      721 tttcttatat attcattgca cagagcccgg cttacagctt ctgcaaagcc gtcagccatg
      781 ctgtcggggt gtcctacccc ttttctttcg acgatttcaa tctcctgttt ctcgataggt
      841 gtttgagtaa ttttttccac acgtatattt cttgccattt aattacctct aaaggttttc
      901 ttcaattgat gctatttacc atataataac tttttgttta tgtaaatcta tttattcagg
      961 ctaatttaat gctggatagt gaatttgaca caatgaaaga aatagcttcg ttatcagaaa
     1021 ccacacgatc actcctgaaa aaacacggtt atcacctcgc tggaacccat agcgccgtga
     1081 agacatgcct ctggctcaac aagagcatcc gtaattccgg ggcatgttat aaatcaaagt
     1141 tctacggaat gaattctcac cggtgcatac agatgactcc cacgcttgtc tgtaatcaca
     1201 ggtgccttca ctgctggagg gctgtggaag tacctgtaga gatccccact gtatgggatt
     1261 caccagaccg gattgtgaat gatgtccttt tggaacacaa acggcttatt tcaggttatg
     1321 gcggttcgga attcatggat aaaaccaggt ggaaagaggc attttcccct aaacatgtgg
     1381 caatctcgct atcaggtgag cccacattat atccctatct tcctgaactg atagaggagt
     1441 tccacaaaag ggaaatgacc acttttgttg tcacaaacgg cacaaatccc gaaatgatag
     1501 aaaaaataag gccaacacag ctttacataa gcctgaatgc accctgtgaa gagatatatg
     1561 aaaaagcatg ttcgccactt gggaatagct gggaaaacat cagacgatca ctggaaattt
     1621 tgaaaggatc gtgtacaagg accgctatca ggataacact gaccaaaggt gtgaatatta
     1681 tagatccgga aggttatgcg cggcttatag aacttgcaca acccgattat gttgaactga
     1741 aagcgtatat gcatcttggc ttttcaagaa agcgcctttc cagggaaaac atgccctcat
     1801 atgatgaggt tcttgatttt tcaagaagag tagcaaaagt gcttgattat tgcatcgcag
     1861 acgattccat gggaagccgt gttgtattgt tgtcgaagga tggcggaaaa acagtaatct
     1921 aacaaaatgc ttatgtgctt tatatgctta ttatcatcag attatgactg aaggagaggc
     1981 gaaggtaatt ccgggagaaa atatactgga agagcccatt gaaattctcg taaatcttgc
     2041 caaaaacggg gaaattgacc catggaatat caatatcgtt gaacttaccg ataaattctt
     2101 aaagagagtt gatgaccttg agaaaatgga tctgcgcgtg tcagggcgaa ctctccttta
     2161 tgcttcggta ttgctgcgca tgaaatcgaa tgctcttgtt gaagttgaag aaccgcctga
     2221 ggagatagat attgagtcgg ataatttcga aataagcgat tatccggtgc cttcaatgcc
     2281 tcttcgccgc tattcaaaaa gaccggtgac acttgaagaa ttattgaccg aattaaagag
     2341 agcggaagca tttgaaaaaa aaaagctcaa tcgggtcgaa aataaaaagg atgaaatcag
     2401 ggccacactt gaggacgttc tttcagtcgc ccatgatgaa gatattgaaa gcaggatcgg
     2461 aaagatgagg tgtatcttga atgaattatt ggataaacaa aaatcgatta aattttcaga
     2521 aatttcttct acccttgaca ggacaggaat tttaatggct tatcttgcga ttttattcct
     2581 tgccacaaaa aaggagatat ggatcgaaca ggacgaattc tttggtgaac ttttcataag
     2641 caggacagca tcatgagcgg ggataaagag atccttgaag ccgcgctttt tgcatcaggt
     2701 gagcctcttg atattggaca gcttaagaac cttgtaagag gaaagaacgt cagggaaatg
     2761 ctccaggaat tagtggatga atacgtagct cgcgatagcg ctattgagat aaaggaaatg
     2821 gatggccggt tcataatgca ggttaaaccc gaatatgcgc aaaaggttcg gtcaatagct
     2881 cctaaagagc ttagatcccc ggtgcttcgg acacttgcaa tgatagccta tcaccagccc
     2941 ataactgtag cagagcttgt taacaggaga ggcgccgcaa catatgacca tgtcagggag
     3001 cttgaagaaa gcggattcat atcggcattg cagcagggaa gaacgcggct tcttcaaacc
     3061 acgcccaggt tcgccgagta tttcaatctt gaatccggcg atcctgaagc tataaaacta
     3121 aagatcatag aacttgccag ggagcagaaa atgggacttg acaagtggct cggaaagcag
     3181 ggaataggtg taagtcccat gtatgaatct ttgatggagc tttgtggtat agcagaatac
     3241 aaagttatta acccgtacgc accgggtgaa gatgagcaag atcgcataca ggaactgggc
     3301 gttcttgtaa tttcaaaagg atataaggaa agagtcagca agtattttga tggaagaata
     3361 attgaaatcc aggcaacgac ttttgaagat cttatcaact ccgtcaatat cctcgctgaa
     3421 tatggcatca ggagaaaaac aaaagagagc atcgaattta tttcggaatt aagagatgaa
     3481 tatatcgaaa aagctaactt cataactgca aaagtcatgc cccaaacaga aatggtatcg
     3541 cgtatgatca atgaaatggg actggggata tctggagatg gtgtaaagat agcccctgat
     3601 tatggcacat cgagtgaggg gaaggagata gggattggag ctgatattct gatacccact
     3661 cataagaatg cacagatgga tattgtgaat aggatttgcc agaggtatga tgcggtgata
     3721 gagggattgg aaaatcggaa taagtagagc tatgatatca tagacccatt gttttgattt
     3781 caaaaccatt ttaaaccgca gatggactat atacgtatgt aggtcctttt aagtaaaaca
     3841 acaactatta aagtaagata agtatgcaac aacaagatta catttttagg attgatgaaa
     3901 aaagcataaa aaacttctcg aaaagcatag ccaatgaggt ggcagataga atctattatg
     3961 aatttctgct tgcgagatat atgccagaag tccttgacat agaaaaaggt agt
//
LOCUS       DATGBN010000247         5736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_330987, whole genome shotgun sequence.
ACCESSION   DATGBN010000247 DATGBN010000000
VERSION     DATGBN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330987"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VKL21_05920"
     CDS             <1..310
                     /locus_tag="VKL21_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HMB45341.1"
                     /translation="EGFNYISNVVEQRSRAHYYPGGSKIRIKLIAEMNTGRILGAEMV
                     GREGVSKRIDVFATAIAAKMTVEDIGDLDLSYAPPFAPVWDPILIAANDLKNKINEAK
                     "
     gene            489..1634
                     /gene="dnaJ"
                     /locus_tag="VKL21_05925"
     CDS             489..1634
                     /gene="dnaJ"
                     /locus_tag="VKL21_05925"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMB45342.1"
                     /translation="MAIKRDYYEILGVDKKASADEIKTAYRKLAMQYHPDRNKAPDAE
                     EKFKEISEAYAVLEDQNKRQQYDQDGHAGIDMRYSQEDIFKDAAPDIEDILRDFGVGG
                     SGKGGSIFDIFSGGGERREGPRRGSDILYELPIDFIEAASGKTVDIEIPRIETCNICH
                     GSGARPGTSPKTCPSCSGTGQVSKAQNTPVGRFMTTSTCGTCHGSGKIIDAPCTTCHG
                     SGTVQGRRKLEVKIPPGVDTGSRLRIPGEGEAGKKGGPPGDLYVKMNVRPHDFFSRRD
                     NDLLMEATITFTQAALGDEIIVPTLDGKVEMKITPGTQNGNVFRLRGKGFSSLHISGK
                     GDQLVKIKIEVPTKLNDRQKQLLRDFAEAGGEKARSEGIFGKVREHI"
     gene            1802..1948
                     /locus_tag="VKL21_05930"
     CDS             1802..1948
                     /locus_tag="VKL21_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45343.1"
                     /translation="MVRLENTIEETEIAEKTKMPKVDKEETERLRKFNEELRSRKAPQ
                     PKAK"
     gene            2500..3201
                     /locus_tag="VKL21_05935"
     CDS             2500..3201
                     /locus_tag="VKL21_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45344.1"
                     /translation="MYLFSWDNVPGKENGKLIEYLEQKYGVDWVRTAKIEKIDNNDIR
                     IATEKNFLSLRLNNENTKVTLTINKVATDEFIVKTENSKLNIYKDTEQEKKDIRQGVK
                     VKDISYPYSLLGGLVLAGLIVVFLMGYYGTQSAGIFSPREAFFVIGASILILTAASVY
                     LFLTLRDTISVLKIHEEELQAARDAAQERRLASLEDTRSGATTSLGPRVTALEPQLAT
                     LETRITALERERSPR"
     gene            3290..3511
                     /locus_tag="VKL21_05940"
     CDS             3290..3511
                     /locus_tag="VKL21_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45345.1"
                     /translation="MEEEESKEINKTLNDLSVKIDNKIEELTKSIEKTLGEKQEYAEG
                     KISENPLAYMAGEFVGGVIIGYMMGKGKS"
     gene            3511..3798
                     /locus_tag="VKL21_05945"
     CDS             3511..3798
                     /locus_tag="VKL21_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45346.1"
                     /translation="MHQSILDFLSGLSKSIIQGISEVQGEIGNSIEERIEKFREKLFI
                     TGVAISIAAIGFFLTLWGIASAIDTMFAMRGLGFVLIGLLAVLTGVLAYKK"
     gene            3846..4118
                     /locus_tag="VKL21_05950"
     CDS             3846..4118
                     /locus_tag="VKL21_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45347.1"
                     /translation="MAYTLEILIILIGIIYGYMRPGKEDRSTLLKKGVIIGIILGAIM
                     VVLGVIGGREILLLGSLVGAVVFIEVIILAVLFIIGTYIGDMLERK"
     gene            4158..4349
                     /locus_tag="VKL21_05955"
     CDS             4158..4349
                     /locus_tag="VKL21_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HMB45348.1"
                     /translation="MKSSTQDQAEGKIHKVKGEIKEISGKHPDLGREGKDVKIVGKVQ
                     EKVGQIKKTFFSTWCFAAS"
     gene            4480..5064
                     /locus_tag="VKL21_05960"
     CDS             4480..5064
                     /locus_tag="VKL21_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45349.1"
                     /translation="MHWREKELLNVLKYGKLTTTEIVGRVNMSKATALKYLDSMKEKN
                     LVDGEEIGTAKIWFLKTGEEVVDNKIKVLVADDDKNIINIIRYSIDSDQFEVLEAANG
                     KEVLGMVFARSPDILILDVMMPEMDGYMVCEELKEHDSTKNLPIIILSAKVSLDDKVK
                     AMGLGIDDYMIKPFDPRELEARIRMRLKLTASKN"
     gene            5476..5598
                     /locus_tag="VKL21_05965"
     CDS             5476..5598
                     /locus_tag="VKL21_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45350.1"
                     /translation="MEEESKQINKTPLAYMAGAIVGGVIVGYVMDRGKSQCCKV"
BASE COUNT         1890 a         1003 c         1361 g         1482 t
ORIGIN      
        1 agaaggtttc aattatattt ccaatgtggt tgagcagaga tcaagagctc attattatcc
       61 tggtggcagt aagatacgga tcaagctaat tgcggaaatg aacactggcc gaattctcgg
      121 agccgaaatg gtcggcaggg agggtgtttc gaagcgaatc gatgtttttg caactgctat
      181 tgcagctaaa atgactgtgg aagacatagg ggacctcgat ttgagttatg cgcctccatt
      241 tgcacctgta tgggatccaa ttttgattgc tgcaaatgac cttaaaaata aaataaatga
      301 agcgaaatag ttggaaagaa tttgtaggaa tgatcgatga actggatgat attccgatta
      361 caaatgttga aaagtgtatt taattttgaa caaaagaaaa cagacaggga agaaaaacaa
      421 atcaaagaag aagaaattaa tctggaacct gaagaaaaag accggtatca ttcaaggtgg
      481 tcattatcat ggctattaaa cgcgactact acgaaattct cggcgtggat aaaaaagcat
      541 cagctgatga gataaagaca gcgtaccgca aacttgcgat gcagtaccat ccggacagga
      601 acaaagcccc tgacgctgag gagaaattca aggaaatatc agaagcctat gccgtactgg
      661 aagaccagaa caagcgtcag caatacgacc aggatggtca tgctggcata gatatgaggt
      721 actcgcagga agatattttc aaggatgcag ctccagacat tgaagatatt ttaagggatt
      781 tcggagttgg cggttctgga aagggcggga gcatttttga tatcttttcc ggtggcggtg
      841 aaaggcgtga aggaccgagg cgggggtctg atatccttta tgaattgccc atagatttta
      901 tagaagccgc ctccgggaaa accgtggata ttgaaatccc aagaattgag acatgcaata
      961 tatgccatgg aagcggggca agacccggaa catcacccaa aacatgtcct tcgtgcagtg
     1021 gcaccgggca ggtgagcaag gcacagaaca cgcctgttgg cagattcatg accacatcta
     1081 catgcgggac atgccatgga agcggaaaga taatcgatgc tccatgcacg acctgtcatg
     1141 gttccggaac cgtacaggga cgccgcaaac tggaagtgaa aatcccgccc ggcgttgata
     1201 caggttcaag actccggatt cccggagaag gcgaagcagg gaaaaaaggc ggacctccag
     1261 gtgacctgta tgtaaaaatg aatgtcagac cgcatgattt tttctcacgt cgtgacaatg
     1321 atctattaat ggaagcgacc ataacgttca ctcaggctgc gctgggcgat gagataatcg
     1381 taccaacgct tgacggcaag gtagaaatga aaatcacgcc gggcacacag aacgggaatg
     1441 tttttcgact gcgaggtaaa ggattttcga gccttcatat atctggtaaa ggggaccagc
     1501 ttgttaaaat taagatcgag gttccgacaa agttaaatga caggcaaaaa cagttgcttc
     1561 gtgattttgc cgaggcaggt ggtgagaagg caagaagcga gggaattttt ggaaaggtga
     1621 gagagcatat ctgaaagcac gcactcccag taaatcgggc ttgctgtttt catattctgg
     1681 gaatggtcat gccatcattc tcatcgtatg agggtggctt ataccattat aatattatga
     1741 aggcattcat tgtattgttt accagcgagt gaacgagaac accaataagg aggtgtgtaa
     1801 aatggttcgt cttgaaaata caatagaaga gactgaaatt gctgaaaaaa ctaaaatgcc
     1861 aaaagttgat aaagaagaaa ctgagaggct aagaaagttt aatgaagaat taagaagccg
     1921 caaagcacca caaccgaaag cgaaatagat aatttgtttt gatttaaaac attttattta
     1981 tccttttttt tcaaagcttg gttatgaact ttgaggcgca ggtgaactac tccctttaaa
     2041 aagaggagct tctcgcttca ttggttctct attgattctg gtttagatat tagaaatgtt
     2101 atttttagct gaaccctaag gtcgagccag ctataacggc ctgcgccctt cgatgattct
     2161 gagcagtacc aagatgatag caatgaccag caatatattg attaagccac caactgaacc
     2221 gagcgatccc aatagccata ggatcaacag aatcacaact aatgtcgtta ttatatccat
     2281 ttttctaacc tccttataga tgctatttga accaaatgtt aataatcttt tcgcttgctt
     2341 ttggctctca gttaacacat atgcatcggc atccaatagg tttatatgtg ataatgcatc
     2401 atttagaaat ggggttacaa ttgttttctg atatatctgt atgctattgg aaatgattgt
     2461 tctctgtaat taattgctat atagcaagga gtggaatata tgtatttatt tagctgggac
     2521 aacgttccgg gaaaagagaa tggaaaactc atagagtatc tggaacagaa atatggtgtc
     2581 gattgggtaa gaacagcaaa aattgaaaaa atcgataaca atgatatacg aatagcgaca
     2641 gaaaagaact ttctttcgct aagacttaac aatgaaaata ctaaggtaac cttaacaatc
     2701 aacaaggttg caactgatga attcattgtg aagactgaaa atagtaaact aaacatatac
     2761 aaagatactg agcaggaaaa gaaagatatc aggcagggag tcaaagtgaa agatatcagc
     2821 tacccctact cattgctagg cgggctggtc ctcgcgggcc tgatcgtcgt gttcttgatg
     2881 ggctactatg gcacccaaag cgccggcatt ttctccccgc gcgaggcttt cttcgtgatc
     2941 ggcgcttcca tactaatcct gactgccgca tcggtgtacc ttttcttgac gctgcgggac
     3001 actatcagcg tcctcaagat acacgaggaa gaactgcaag cagctcgtga cgcagcgcag
     3061 gaaaggcgtc tcgcctcgct tgaggacacg cgctctggcg ctaccacctc gctggggcca
     3121 cgcgtcacag ccctggagcc gcagcttgcg accctggaaa cacgcatcac cgccctggag
     3181 cgtgagcggt ctccccgctg aggccgcaaa gccggactga aaagtatgta tatgtgtgtg
     3241 tatatataca catatatgca cacataaatt atattataga ggttatttta tggaagaaga
     3301 agaatcaaaa gagataaata aaacactcaa tgacctgagc gtaaaaatag ataacaaaat
     3361 agaagaactg actaaatcaa ttgagaagac gcttggggaa aaacaagaat atgcagaagg
     3421 gaagataagc gaaaatcctc ttgcttatat ggcaggagaa ttcgtcggcg gagtgataat
     3481 tggctatatg atgggcaagg gaaaaagcta gtgcaccaaa gtatattaga ttttcttagc
     3541 ggcctttcaa aatcgataat tcagggtatt agcgaagtgc aaggtgaaat tggcaattcc
     3601 atagaagaac gtatagagaa attcagagag aaattattta ttaccggtgt tgcgatctca
     3661 atcgcagcca tcgggttttt cttgaccctc tggggaattg catccgccat tgatactatg
     3721 ttcgctatgc gaggcctggg atttgtactg ataggtttac ttgcagtact gaccggggtg
     3781 cttgcatata agaaatagaa ctggctggaa cagaaataga ataaaattta aatcaggagg
     3841 ataatatggc atatacactg gaaatattga taattttaat cggaattatt tatggctaca
     3901 tgaggccagg caaggaagac cgttccacat tattgaaaaa aggagtgata attggaataa
     3961 ttctgggcgc tataatggta gtattgggag ttatcggagg tagggaaatt ctactgcttg
     4021 gcagtttggt tggagcagtc gtatttattg aggtcataat ccttgcagta ctattcatta
     4081 ttggcacata cataggagat atgttggagc gaaaatagat aaaaaattgt gggattaata
     4141 ataaacggag gaataccatg aaaagcagta cacaggatca ggcagaaggc aagatccaca
     4201 aagtgaaggg tgagatcaag gaaatctcgg ggaaacatcc cgacctggga agggaaggca
     4261 aagacgtaaa gatagtcggc aaagttcaag aaaaggtcgg tcagatcaag aagacctttt
     4321 tttcaacatg gtgctttgca gcttcttaaa tattcattaa acttttttag cctttcaact
     4381 ataacatttc ctgcatattt tatacttcca ccttgcaaag attaaatagc tataaatcaa
     4441 tataggtcat ttaaatttaa acaaacataa agggttaata tgcactggcg agaaaaagaa
     4501 ttgcttaatg tattgaaata tggaaaattg acgaccaccg agatagttgg aagggtaaat
     4561 atgagcaaag caaccgccct gaaatatctg gatagcatga aagaaaaaaa cctggtggat
     4621 ggggaagaaa taggcactgc aaagatctgg ttcttaaaaa ctggagagga agtagtcgat
     4681 aacaaaataa aagtactggt tgcggatgat gataagaata ttataaacat tatccgatat
     4741 tctatagata gcgatcaatt cgaggtttta gaggccgcaa atggaaaaga agttttgggg
     4801 atggtctttg ccagatcgcc tgatatcctg atcctggatg tcatgatgcc tgaaatggat
     4861 ggctatatgg tctgcgaaga attgaaagaa cacgattcta caaagaattt gcctataata
     4921 atcctgtctg ccaaggtcag cttagatgat aaggttaaag caatgggttt aggcatagat
     4981 gattatatga taaaaccttt tgatccgagg gaactggaag caagaataag aatgagattg
     5041 aagctgactg ccagtaagaa ttaagatggt tcttttggat tcagatcctg cctataaatt
     5101 ttaattaata tgattatgcc tgcctcatga ataatattgt tatgataggt taggtggctt
     5161 ctatgtaggc aggcacggta aattcccagt cccatggttc acccgctggc aaacaatata
     5221 acaaacaact ccatactatt taaaatgttc taatcgttca tttattttta aacaataaga
     5281 tacaattgtt gacggaaaat aagatattat ctattccaat gtcattcttg atgtgtcatg
     5341 agagtggagc caatttggga gtctcgtggt tcatcgccag taaggctcaa aagtgttgat
     5401 tcatggtggc cacaatgccg gactgtaaag tatatatatg caaaaataca taaattacat
     5461 tgtggaggtt actttatgga agaagaatca aagcaaataa ataaaactcc tcttgcttat
     5521 atggcaggag caatcgttgg cggagtgatc gtgggttacg tgatggaccg gggaaaaagc
     5581 cagtgctgca aagtataatg gattttctta gcggcctttc aaaatcgata attcagggta
     5641 ttagcgaagt gcaaggtgaa attggcaatt ccatagaaga acgtatagag aaattcagag
     5701 agaaattatt tattaccggt gttgcgatct caatcg
//
LOCUS       DATGBN010000248         9731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_485859, whole genome shotgun sequence.
ACCESSION   DATGBN010000248 DATGBN010000000
VERSION     DATGBN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485859"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VKL21_05970"
     CDS             <1..1122
                     /locus_tag="VKL21_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HMB45351.1"
                     /translation="KISSLLGIPEHSITGALKRKTLQYVYGKQELFRFDKPVSSVEGG
                     TAVFLEPFKIIRGFPKISRTLMLYPALLKHFSSCQKVAVEEKMNGYNVRVALIGDKLV
                     ALTRGGFICPYTTEKVNYLIGRDFFQDHPDLVLCGEMVGPDSPYVPKTFYNIESLDFF
                     AFDVRKINSGYPMSVMERRKLMDEYGIKSVRLYGEFEVAGAHEAITDIIKDLGNTLRE
                     GVVIKDPQMILHPVKYTSSLSNCADLKYAFEFYNDLGRDFFFGRVCREAFQSVEWDEN
                     EESMRKRCQELGESILLPMISTITKKKAGERIAENVQIRVKNPKTIAEFEDYLRLLGV
                     DAVFEMPEKIGDEYLVRIRKIYQSTNDKTETILGGQMWS"
     gene            complement(1146..3113)
                     /locus_tag="VKL21_05975"
     CDS             complement(1146..3113)
                     /locus_tag="VKL21_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HMB45352.1"
                     /translation="MRLIFSILAVMIVVLFTVESAIADDCETIENQEICWEDIASTTL
                     GWTYPQVTQGGYIIEVRDFDWLGSISMRVSKNGIVKEVVLTEGESYLFDFSNDSAFEG
                     IEIIADQVSNINSFPSNIGTFPKDPQAKISFKLSIPDGKKKPTLELEVSTERKIDNDF
                     VLTAYINTQNSGESDLVDTQVWILFDGLEIINEFDFEKGSMNELTSSDYEIKWENISS
                     YKLTPTNPGIIKNGYIINVINFLNEKARINISYNGYIKSDELVEGGSVVFGFTRENEY
                     TGIRILGTHISNDTAELILQYPKKNSVKQRYPIIIAGSSESIKLGFQIPLSSRKTYTI
                     SAISTAKDSQGNNYTQSVSTKISLKDTFKINKITSDSILGKSLYSKYSRAGDIATIKN
                     RTYVMIRVDNLANYPVHNVKLTDTILPGFEEDGNRTSMSWNFDMNARDHKEFTYTIIA
                     KRQGVYNLPRAELTWNEFQEDLSLESNSPRTIVSGPHIVMERSFNKSNINIGDTLLAS
                     LSITNNGDMPINIIVNDSVPQNTTFLSGTLSFSGLLRPSESARIVYAITANDTVIEFQ
                     APEMRSNHQGFEWYEPLPSKKISGYSPVPAATPTIIPAETAKMPEQGMVRMVDEGFPW
                     LEGAISIITLVSGIFLLMMLNKKKYFRTNEK"
     gene            3316..3388
                     /locus_tag="VKL21_05980"
     tRNA            3316..3388
                     /locus_tag="VKL21_05980"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3349..3351,aa:Asn,seq:gtt)
     gene            3434..3508
                     /locus_tag="VKL21_05985"
     tRNA            3434..3508
                     /locus_tag="VKL21_05985"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3469..3471,aa:Ile,seq:cat)
     gene            3639..4616
                     /gene="ftsZ"
                     /locus_tag="VKL21_05990"
     CDS             3639..4616
                     /gene="ftsZ"
                     /locus_tag="VKL21_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB45353.1"
                     /translation="MTLDSRIIELAKAAKPAVAIIGLGGAGCNIVSWIAQKEIAGSKI
                     IASDTDAAHLLEVKADVKILMGEEAYHGKGCGGYAERGTGAAKESIKEIKKELLGSNL
                     IFIIAGMGGGTGTGSATVVAEATHEVGILTIGCVMMPFGYELLKRKKAILGIKSLAEK
                     CDSLVVIDNSRLRAIVGTLPLREGFAIANEFVGSFIKNISETITHASLVNLDYSDLRS
                     VVERGGISSIGIGEAQGENRIQKAVELALASPLLDVSDISGTYGVLIHITGGEDMTLG
                     EVTQAGEIILQKAPNTGRIIWGAKVDESIEGTVKVIAVLTGVLDPKKLV"
     gene            4669..5637
                     /locus_tag="VKL21_05995"
     CDS             4669..5637
                     /locus_tag="VKL21_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HMB45354.1"
                     /translation="MFLSLKESIDNLCDYTYNFTWQNNTIRPGNIIPSQTGTNYTFSD
                     LVNELKKGKDVHISGNAGSGLGYSMGVNLKHFGGTGAIEKAGKLYIDGDVSSEMGMGM
                     VSGTIYVKGNVEQPHGNVIEVISDDPGFRKFRSITDILCHGTGKDVLIKNNYDVRKKH
                     LLLIDGIPRGTIAARCDCEGFVSIEGDVYNGTGLLMQKGKIHVSGDAGMNTGAHLDGG
                     LIIVEGETGEFAGAYMKKGIIILKDARGYAGANMKNGLILSKNKVKVSPPVEELERTQ
                     EDANMIIKHLGIGHVEAMSYHKYGLVKERLIRMRDGSVVVRRDDED"
     gene            complement(5634..5963)
                     /locus_tag="VKL21_06000"
     CDS             complement(5634..5963)
                     /locus_tag="VKL21_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45355.1"
                     /translation="MGVNESVLDEISQERAQYLVNTDDIMYEMYCEPIFDKRTGILKE
                     DPYYSSNIERVNKIKVVDQLFTSKEYQTFIQLTRPEDISKQKFQPIPPKKLKNEELQD
                     FDTTIED"
     gene            6257..7348
                     /locus_tag="VKL21_06005"
     CDS             6257..7348
                     /locus_tag="VKL21_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45356.1"
                     /translation="MVKKSEEKTSNKGTTEVKNAINLWVNNSNEFLKLWGDSNLKLYK
                     PWIEFMGEKSMKINEMASSTSPLKYKEFYEDWMKTYRSTFGDISHAEISSPEEALEGF
                     VKCANESNKLYMSWIQEFGENSKKTAEVLNNGRDPAKYRECCDSWMNTYEKVIDDINE
                     HPAIKYQRDIFGSYTGMPDLYSESIAKMAKQIKEVYTRLYVPTEESINKLSDKMENLS
                     KGDVNPETYKEFYDMWLNTYKENFSTLFDPQTMKPSKELLDNLKESTEININMFKSWT
                     EALEKLSAKMEDQSKFINDPEAFKEFHHLWAKMFEKTSEDIFEGVPLTSPLKEMMEPV
                     KSACKIYTDTSIKMSQMWMDSFSIIAPKV"
     gene            7351..7890
                     /locus_tag="VKL21_06010"
     CDS             7351..7890
                     /locus_tag="VKL21_06010"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HMB45357.1"
                     /translation="MEDTESESTREFLDFWVKTYDTAFGKISEIPALGPMREKSEKMS
                     KGLPLFFSLYSTWMDTVSDFQAISIEAMKRMNTKTVSMEGKTGIENYKELYNVWMESY
                     SETFKEFLKSGHFSSDMGKFMSNFLDVQKYNRDMLEENYLKPSNLPTKSDIDELNKEL
                     YSLKKQVKELSSKINTARE"
     gene            7912..9069
                     /gene="phaC"
                     /locus_tag="VKL21_06015"
     CDS             7912..9069
                     /gene="phaC"
                     /locus_tag="VKL21_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HMB45358.1"
                     /translation="MYELNSIEKEYTRLTSGLEIFMKMPEISVGTTPHEIVYTEDKMK
                     LLHFFPTVEKTHPVPILMVYALVNRYYILDLQPDKSVIKKLLDDGFDVYIIDWGYPSG
                     TDRYLTLNDYVNGYVNNAVDLIRKRSGLDKITLLGVCQGGTFSIMYSSLHPEKVKNLV
                     TLVAPVNFDSKKGLLHIWAKSLDVDKIVDYYGIIPGDFLNSGFLLTDPFRLMIDKYVG
                     MFDRIECEPGDQACLIRNEEIVNNFMRMEKWIFDSPDQAGETFRQFMKDCYQKNLLIQ
                     NKLVINGKKINLKNITMPLLNIMAEFDHLVPNEASIPLNDAVSSKEKEMVVFPTGHIG
                     IFVGSKSQKEVCPTISKWLTPRSGLDEKGPHDPEIKNHKRKSKEALHEGKR"
     gene            9053..>9731
                     /gene="fabG"
                     /locus_tag="VKL21_06020"
     CDS             9053..>9731
                     /gene="fabG"
                     /locus_tag="VKL21_06020"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMB45359.1"
                     /translation="MRVKDKVVIITGGGSGIGKETGLLFAKEGAKVVVADVNEKAGAE
                     TVDTIKNAGGEAVFAKLDVSNREQTKQVVKDTIAKYGRIDVLINNAGIIQDALVVKMT
                     EEQWDKVININLKGPFNCIQAVAEQMIAQGSGVILNISSIVALYGNVGQTNYAATKAG
                     LVGMTKTLAKELGKKGIRVNAIAPGFIYTPMTATVPEKILDMMKEKTPLKRLGTPGDV
                     ANALLFLS"
BASE COUNT         3085 a         1742 c         2140 g         2764 t
ORIGIN      
        1 aaaatatcca gcctcctggg tattccggaa catagtatta caggggcgct gaagcgaaag
       61 acgttgcagt atgtatatgg gaaacaggag ttattcaggt ttgataagcc tgtttcttcc
      121 gtggaaggag gaactgcggt attccttgaa ccatttaaga taatacgcgg ttttcccaaa
      181 atatcacgta ccttgatgct gtatccggcg ctgcttaagc atttttcttc ctgccagaaa
      241 gtggcggtcg aagagaaaat gaacggctac aacgtcaggg tagcgcttat cggcgataag
      301 cttgtagcgc tcacacgtgg cggttttata tgcccttata ccaccgagaa agtaaattat
      361 ctgatcgggc gtgatttttt ccaggatcat cccgaccttg tgctttgcgg tgagatggta
      421 ggaccggata gcccttatgt acccaagaca ttttacaata tagagtctct tgattttttt
      481 gcatttgatg tgaggaagat aaattctggt tacccaatga gtgtcatgga aagacgaaaa
      541 cttatggatg aatacgggat caagtcagtg cgtctttacg gagaatttga ggttgcagga
      601 gctcatgaag caataacaga tatcataaag gatttaggga atactttgcg agaaggcgtt
      661 gtcatcaaag accctcaaat gatcctgcat cctgtcaaat acaccagctc gctaagtaat
      721 tgcgctgacc tcaaatatgc ttttgaattc tacaatgatt tagggcgcga ctttttcttc
      781 ggaagggtat gcagggaagc attccaatct gtagaatggg atgagaacga ggaaagcatg
      841 agaaaaagat gccaggaact cggtgaaagc attctcttgc ctatgatttc taccattaca
      901 aagaagaaag ccggtgaacg cattgctgaa aatgtgcaga taagggtgaa aaacccaaaa
      961 acaatcgcgg aatttgaaga ttacctgagg ctgctggggg tagatgctgt ttttgagatg
     1021 cctgaaaaaa taggggacga atatcttgtc aggataagga aaatatacca gagcaccaac
     1081 gacaagacag aaactatact tggcggccag atgtggagtt aacccaagaa agatcaggaa
     1141 atatttcatt tttcatttgt tctgaagtat tttttcttat tcaacatcat aagaaggaaa
     1201 attccggaaa caagcgtaat tattgaaatc gccccttcta accaggggaa cccctcatct
     1261 accattcgga ccattccctg ttcaggcatt ttggcagtct cagcagggat tattgtcggg
     1321 gtggcggccg ggacaggaga atatcctgat attttttttg atggaagagg ctcataccat
     1381 tcgaatcctt gatggttcga tctcatttcc ggcgcctgga attcaattac agtatcatta
     1441 gcagtgatgg catagacgat ccgggcgctc tcagagggtc ttaacaaacc ggaaaaagat
     1501 agcgtgcctg aaagaaatgt tgtattttga ggcacactat cattaactat gatatttatt
     1561 ggcatatcac cattgttggt tattgaaaga gaagccaaaa gtgtatcccc aatgttaata
     1621 ttacttttgt tgaaactcct ttccattact atatgcgggc ctgatacaat tgttcttgga
     1681 ctatttgatt ccaaactaag atcttcctgg aattcgttcc aggtaagctc tgctcttgga
     1741 agattataaa ctccctgcct ttttgctatg atcgtataag taaattcttt atgatcgcgg
     1801 gcattcatat caaaattcca ggacatagaa gtcctgtttc cgtcctcttc gaaaccaggc
     1861 agaatagtat ccgtgagttt tacattatgc acaggataat tcgccagatt atctacacgg
     1921 atcataacat aagttctatt tttaattgtt gcaatgtcac ctgccctgga atatttagaa
     1981 tataggctct tcccgaggat agaatcggaa gtgattttat ttattttaaa ggtatccttt
     2041 agggagattt tcgttgagac actttgtgtg taattatttc cttgactgtc ttttgcggtg
     2101 gaaatagctg atattgtata ggtttttctt gaggataacg gtatttgaaa tcctaatttg
     2161 attgattcac tgcttcctgc aatgataatt gggtatcttt gtttcacgct atttttctta
     2221 ggatactgca atatcaattc tgctgtatca tttgaaatat gtgtaccaag tattcttatc
     2281 ccggtgtatt cgttctccct ggtgaaacca aatacaacag aaccgccttc cacgagttca
     2341 tcacttttta tatatccatt gtaactgata tttattctgg ctttttcgtt caaaaaattt
     2401 attacgttta taatataccc attcttgata atgccggggt ttgttggtgt aagtttatat
     2461 gaacttatat tttcccattt gatttcataa tctgatgaag ttaattcgtt cattgaacct
     2521 ttttcaaaat cgaattcatt aataatttca aggccatcaa atagtatcca gacttgcgta
     2581 tctacaaggt ctgattcccc tgaattttgc gtattaatat aagcagttaa tacaaaatcg
     2641 ttatctattt ttctttctgt ggagacttct aattcaagag tgggcttttt ttttccatcg
     2701 gggatagata gtttgaatga aatttttgcc tggggatctt ttggaaatgt tccaatattt
     2761 gagggaaatg aattaatatt tgaaacctgg tctgcaatta tttcaattcc ttcgaaagca
     2821 gaatcattgg aaaaatcgaa taaatatgat tccccttctg ttaatactac ttcttttact
     2881 atcccgtttt tgcttactct catagaaatt gagcccaacc agtcaaaatc cctgacctct
     2941 attatatatc cgccttgtgt tacctgtggg tatgtccagc ccagggttgt acttgcaatg
     3001 tcttcccagc aaatctcttg attttcaata gtctcacaat catcggcaat tgctgattct
     3061 acagtaaata agacaacaat cattacggcc aggatagaaa atatcagtct catgttttta
     3121 tttgtctaag aaatttagca gcaaatcgtt ttgacatact atcgttccgt caggtatggt
     3181 aggttgtgcc caatcggaaa tgatgcgtat catcataatg atattatggt cttcttatat
     3241 aaaaaagcta tccattaact tgacattatt aacaaaaagc ttaaatcaaa gtatgccttt
     3301 tagggttcgc tttatgccgc cataactcag ctggtagagt gcctggctgt taaccaggat
     3361 gtcacaggtt cgagccctgt tggcggcgct ttggtattga atggtttaac taaatttccg
     3421 atgcgattct aaagggcctg tagcttagtc aggttagagc gcccggctca taaccgggcg
     3481 gccaccggtt caaatccggt caggcccatt atttattttt tgatagtcta aagaaaagga
     3541 ctataattga aacatgttag ttaattatat atcttaatac agagataaaa agactattaa
     3601 ctggagtaca aggaaaactt tttcacagga agatttttat gacactcgat tcaaggatca
     3661 tagagcttgc aaaagcagca aaacccgcag tggcgatcat cggtctaggc ggagcaggat
     3721 gcaatatagt ctcatggata gcgcaaaaag agatagcagg aagtaagatt atcgcatcgg
     3781 ataccgatgc ggctcattta ttggaagtga aagccgatgt gaagatcctg atgggggaag
     3841 aggcctatca tggaaaaggg tgcggcggtt atgcagaaag aggcacaggg gcggcaaaag
     3901 agagtatcaa agagataaaa aaggaactgt tgggctcgaa tctgatattc attattgcag
     3961 gaatgggagg aggcactggt acaggctctg ccacagtagt tgcagaggcg acacatgaag
     4021 tcggaatatt aacaataggc tgcgtcatga tgccattcgg ctatgaactc ttaaagcgaa
     4081 aaaaagctat cctgggaatt aaatcccttg ccgaaaaatg tgattccctg gtcgtgatcg
     4141 ataattcaag gctgagagcc attgtaggga ccctgccgct acgtgaaggc tttgcaatcg
     4201 cgaatgagtt cgtgggatca ttcatcaaga atatctctga aactattacc catgcaagtc
     4261 ttgtgaacct tgattattcc gatcttcgaa gcgtagttga acgtggagga atatcttcga
     4321 tcggaatagg cgaagcgcag ggtgaaaatc gcatccagaa agcagttgaa ctggctcttg
     4381 cttcaccgct tcttgatgta tctgacattt caggcaccta cggggtgctt attcatatta
     4441 ccggcggcga agatatgacc cttggtgaag taacgcaagc cggtgagatc attctccaga
     4501 aagctccaaa taccggccgc atcatatggg gagcaaaggt tgatgaatcc attgaaggaa
     4561 cagtaaaagt tatagctgtc ctgacaggag ttctggatcc aaagaaatta gtataagcat
     4621 aaacctgtga catatattat ttaaactcga attcagggat tataccgcat gttcctatcg
     4681 ttgaaagaaa gcattgataa cctgtgtgat tatacataca atttcacatg gcaaaacaat
     4741 acaatccgcc ccggtaatat catcccttcc cagactggca ctaattacac tttttccgac
     4801 cttgtgaatg aattgaaaaa gggaaaggat gtgcatattt cggggaatgc aggctcagga
     4861 ttgggttaca gcatgggagt gaacctcaaa catttcggag ggacaggggc tattgaaaag
     4921 gctggaaaac tttacatcga tggcgatgta tcatccgaaa tggggatggg tatggtttcc
     4981 ggaacaatat atgttaaagg aaatgtagag cagccacatg gaaatgtgat cgaggtgata
     5041 tctgatgatc cgggttttcg gaagtttcgt tccataacgg atatcctgtg ccatgggacc
     5101 gggaaagatg tgcttataaa gaataattac gatgtgcgaa aaaaacattt acttctcatt
     5161 gacgggatcc cgcgtgggac aatagcggca agatgcgatt gcgagggttt cgtttcaata
     5221 gaaggagatg tttacaacgg gacagggctt ttgatgcaga agggaaagat ccatgtctca
     5281 ggagatgcag gtatgaacac tggagcgcac cttgatggag gcctcataat agttgagggt
     5341 gaaacaggtg aatttgcagg tgcttatatg aagaaaggaa taatcattct aaaagatgcc
     5401 agaggatatg ccggagccaa tatgaagaat gggctgattt tgtcaaaaaa caaagtcaag
     5461 gtctcgccgc ccgtagaaga acttgagagg acgcaggaag atgccaacat gataatcaaa
     5521 cacctgggga ttggtcatgt cgaagctatg agctatcata aatatgggct agtgaaagag
     5581 aggcttatca ggatgagaga tggatcggta gttgtgagaa gagatgatga agattaatct
     5641 tcaattgtag tatcaaagtc ctgcaattct tcatttttta atttcttagg gggaattggc
     5701 tggaattttt gtttagaaat atcttcaggg cgtgtcaatt gaataaatgt ctgatattcc
     5761 ttgcttgtga aaagctggtc aacaactttg atcttattga ccctctcaat attactggaa
     5821 tagtaagggt cttctttcaa aatcccggtt ctcttgtcga aaattggttc acaatacatt
     5881 tcatacataa tatcgtctgt attaacaaga tactgggctc tctcttggga tatttcatct
     5941 aatacacttt cattaactcc catattctca cctataaagt ataggctttc actatttata
     6001 tagttttcgt ttgtgagttt atccgtacgg tcttaattat tatagttgac ttgaattcgg
     6061 ggctggtatt caatattatt ttagaacata acataattgc atatggtttc aattccacac
     6121 gattaatatg aataaataaa ctttatatat cattataatc atgttattga tataaaaaat
     6181 gggggttatt acaaccgtgt atatacccga attttattgc catcgaaata aaataaataa
     6241 aaggagtgaa atagatatgg ttaagaaaag cgaggagaaa acatcaaaca aaggaacgac
     6301 tgaagtgaag aatgcgatta acctgtgggt taataattcg aacgaatttt taaaattgtg
     6361 gggggattca aatctgaaac tctacaaacc atggatagag ttcatgggag agaagtctat
     6421 gaaaattaac gaaatggcca gttccacttc gccactgaaa tataaagaat tttatgaaga
     6481 ttggatgaaa acatacagga gtacatttgg tgatatctcc catgctgaga tttcatctcc
     6541 tgaggaagcg cttgaaggtt ttgttaaatg tgcgaatgaa tcaaacaaat tgtacatgtc
     6601 ctggatacaa gagttcgggg aaaattcaaa gaaaaccgcc gaagtcctga acaatgggcg
     6661 tgacccggca aaatacaggg aatgctgtga cagctggatg aatacttatg aaaaagttat
     6721 cgacgatatc aacgaacacc cggcgatcaa ataccagaga gatattttcg gaagctatac
     6781 tggtatgcct gatctctatt cagagtcaat agcaaagatg gccaaacaga tcaaagaagt
     6841 atataccaga ttgtatgtcc caactgaaga gtccatcaat aaactttcag ataaaatgga
     6901 aaatttatcc aaaggcgatg tgaaccctga aacatataaa gaattttatg atatgtggct
     6961 aaatacttat aaggaaaact tttcaacgtt gttcgaccct cagacgatga aaccttcaaa
     7021 agaactgctg gataatctta aggagagcac tgaaataaat ataaacatgt tcaaatcctg
     7081 gacagaagcc cttgaaaagc tatctgcgaa aatggaagac cagtcgaaat ttatcaatga
     7141 tcctgaagct tttaaagaat ttcatcatct ctgggcaaag atgtttgaaa agacatctga
     7201 agatatcttt gagggagttc ccctgaccag cccattaaaa gagatgatgg aacctgtaaa
     7261 gagcgcatgt aagatctata cggacacatc tatcaagatg tcacaaatgt ggatggattc
     7321 gttcagcatt atcgccccaa aggtatgaat atggaagata ctgaatctga atccacacgt
     7381 gaatttcttg acttttgggt aaaaacctat gatacagcat ttggcaagat ttccgaaatt
     7441 cccgctttag ggccaatgcg ggagaaatcc gaaaaaatgt ctaaagggct tcctcttttc
     7501 tttagccttt attcaacatg gatggataca gtctcggatt tccaggctat ttccatagaa
     7561 gcaatgaaac gaatgaatac caaaacggta tcgatggaag gcaagacagg cattgaaaat
     7621 tataaggaac tttataatgt ctggatggaa tcctatagtg aaacgttcaa agaattcctt
     7681 aaatccggcc atttctcttc agatatggga aaattcatgt caaattttct ggatgttcaa
     7741 aaatacaacc gggatatgct tgaagagaat tatctgaaac catccaatct tccaacaaaa
     7801 tctgatatcg atgagttaaa taaggaatta tattccttaa agaaacaggt aaaggaatta
     7861 tccagcaaaa taaacactgc ccgggaataa acggttctca aggggaacat tatgtatgaa
     7921 cttaattcaa tagaaaaaga atatacaaga ttaacgagtg gattagagat ttttatgaaa
     7981 atgccagaaa tatcggttgg cacaacccca catgaaatag tatataccga agataagatg
     8041 aagcttcttc attttttccc caccgttgag aaaacgcatc cagtaccgat attaatggta
     8101 tatgcgcttg tgaaccgcta ttatatcctt gacctccagc ctgataaaag tgtgataaaa
     8161 aagctattgg atgatggatt tgatgtgtac atcatcgatt ggggatatcc ttccggaacg
     8221 gatagatatc ttacattaaa tgactatgtc aatggatatg taaataatgc agtggactta
     8281 ataagaaagc gttcaggatt ggataagatc acacttctcg gggtttgcca gggtgggact
     8341 ttttctatca tgtattcttc attacatcct gaaaaagtca agaatcttgt aacactcgtg
     8401 gctcctgtca attttgattc gaaaaaagga cttcttcata tctgggcaaa gagtcttgat
     8461 gttgataaga tagtagatta ttatggaata atacccgggg atttcctgaa ttccggtttc
     8521 ctgttgacag acccttttcg gcttatgatc gacaagtacg taggcatgtt cgacagaata
     8581 gaatgcgaac ctggtgatca agcctgtctt atccgaaacg aggaaatagt caataatttc
     8641 atgagaatgg aaaaatggat atttgacagt cctgaccagg ccggtgagac attcaggcaa
     8701 ttcatgaaag attgttatca aaagaatctg ttgatacaga acaaactggt tattaacgga
     8761 aaaaagataa acctgaaaaa tatcaccatg ccccttctta acatcatggc ggaatttgac
     8821 caccttgtgc ctaatgaggc aagtataccg ctcaatgacg cagtatcaag caaagagaaa
     8881 gagatggtgg tttttcccac cgggcatata ggcatattcg taggatcaaa atcccagaaa
     8941 gaagtttgcc caacgatttc aaaatggttg acgccaaggt caggtcttga tgaaaaagga
     9001 cctcatgacc ccgaaatcaa gaatcataaa cgaaaatcaa aggaggcatt acatgagggt
     9061 aaaagataaa gtcgtaatta tcactggagg tggaagcggt atcggaaaag agacaggtct
     9121 cctctttgca aaagaaggag ctaaagtcgt ggtcgcagat gtaaatgaaa aagcgggggc
     9181 agagacggtc gatactataa aaaatgcagg aggcgaggcc gtatttgcaa aactcgatgt
     9241 ttccaatagg gaacaaacaa agcaggtcgt gaaagatact attgcaaaat atggcaggat
     9301 cgatgtactt atcaataatg cgggtatcat ccaggatgct cttgtagtga aaatgaccga
     9361 agaacagtgg gataaagtca tcaatatcaa cctgaaaggg ccttttaatt gtatccaggc
     9421 agttgctgaa caaatgatag cccagggaag cggtgtgatc ttgaacattt catcaatagt
     9481 tgccctttat ggcaatgtgg gacagacgaa ttatgctgca accaaagctg gtttagtagg
     9541 aatgacaaag acccttgcaa aggaactggg gaaaaaaggc ataagggtca acgcgattgc
     9601 acccggattc atatataccc cgatgacagc gacagtgccg gaaaagatcc tggatatgat
     9661 gaaggaaaag acgcctttaa agaggctggg cactccgggg gatgtagcca atgcattgtt
     9721 attcctgtct t
//
LOCUS       DATGBN010000249         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_331111, whole genome shotgun sequence.
ACCESSION   DATGBN010000249 DATGBN010000000
VERSION     DATGBN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331111"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VKL21_06025"
     CDS             <1..270
                     /locus_tag="VKL21_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HMB45360.1"
                     /translation="YMVFLSINETHIHISVLIISYTISMISGWLPLFLPGGLGIVDGT
                     MAILFIYGGVPVEMAVLATLLYRLSSYWFNTLLGAFYFWNELKSG"
     gene            complement(256..1152)
                     /locus_tag="VKL21_06030"
     CDS             complement(256..1152)
                     /locus_tag="VKL21_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMB45361.1"
                     /translation="MKILLAEYAVGAGIEEFMLEGKAMLHTLLRSFVTCGHEVIYPSS
                     GIKLEAGKAVMTTGFEETLRKLSRKCDAALVIAPDELLGDLTEIIENNTVNLGCPPDS
                     VRLAADKLKCTRILSREKIPVPETIGHGKYDGDFVIKPRFGCASEGISRSRKGTLKKN
                     FIATGFIEGEHLSISLITGKTQLPLTVNRQLIETGENFSYKGGIVPYHCGRNEEIIDV
                     AKKTARILGCRGYAGVDIVLGDRPYVIDVNPRPTSSIIGISRVMKTQIAELILKSRFG
                     DLPDHVKINGNFTFKKEDLSGF"
     gene            complement(1179..1718)
                     /locus_tag="VKL21_06035"
     CDS             complement(1179..1718)
                     /locus_tag="VKL21_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HMB45362.1"
                     /translation="MKILTHVCCAPCFTYPHKRMLEEGHDVTAFFYNPNIHPYQEYKN
                     RLVSVEKYVELKGTRVIYDNDYDIENYLRGALVAEERCEFCYKERLEKTARIAGQLDF
                     DAFTTTLLISPYQKHETIVTVGKKIAGESGIEFYYEDFREGYQESRDISKTLGLYMQK
                     YCGCIFSEKERYFRKKPQF"
     gene            1983..3137
                     /locus_tag="VKL21_06040"
     CDS             1983..3137
                     /locus_tag="VKL21_06040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMB45363.1"
                     /translation="MRSIKDEKFESPSEIQEKSIPLIIAGKDIIAGSATGSGKTLAFA
                     SGILKNTEKGKGIQALVLTPTRELAEQVAQALAKFSKYKPLEIIAVYGGVGINPQIRG
                     LKTADVVVGTPGRLLDHIARNTLRLGKVKTLVLDEADRMFDMGFKDDVEKIIRKCPEE
                     RQTLLFSATITKEVVLLSKRYMQKPLQVSVDSFVDPKKLVQTYYDVADEQKFSLLLHL
                     LKNESSDLVMVFCNTKRNTDKVTKNLIFSGIDALAIHGGLTQYKRNDVMQRFHTGKFC
                     VLVCTDVAARGLDIRGVSHVYNYDIPRESKQYIHRIGRTARAGAEGKAINILSRTDYD
                     NFGRVLKENDVEITEEILPAFEKAAIRKPERRATKPIIRKAQPHNRNRRR"
BASE COUNT         1045 a          677 c          674 g         1066 t
ORIGIN      
        1 tatatggtgt ttctttccat aaatgaaact catattcata tcagcgtatt gatcatttca
       61 tatactatat ctatgataag tggatggctc cctctttttc tgccaggggg acttgggatt
      121 gttgacggta ctatggcaat acttttcata tacggcggtg tgcccgtcga aatggccgtt
      181 cttgcaacac ttctttacag gctttcatcc tattggttca atactttatt gggagcgttc
      241 tatttctgga atgaattaaa atccggatag atcttctttt ttaaaagtga aattaccatt
      301 tattttcaca tgatcgggaa gatcaccgaa tcttgacttt aaaatcagct cagctatttg
      361 agtcttcatc actcttgata tccctattat tgatgatgtt ggtctgggat tgacatctat
      421 tacatacggc ctatcaccaa gcacaatgtc cacgcctgcg taaccccggc acccaaggat
      481 ccttgcagtt ttttttgcca catcgattat ctcttcattc cttccacaat gatagggaac
      541 aattcctcct ttataagaaa aattctctcc agtttcgatc aactgcctgt taactgtaag
      601 tggaagctgt gttttcccgg ttattaaact tatacttaaa tgctcccctt caataaatcc
      661 agttgctatg aaattttttt ttaaggttcc ttttctactc ctgcttattc cctcggatgc
      721 gcagccaaac ctgggtttta tcacaaaatc cccgtcatat tttccgtgtc cgatggtttc
      781 cgggacaggg attttttcac gggaaagaat acgggtacat tttaatttat ctgccgccag
      841 gcgcactgaa tccggcgggc atccaagatt tactgtattg ttctctatta tctcggtcag
      901 gtccccgaga agttcatcag gcgcaatgac taaagcagcg tcgcatttcc gtgacaattt
      961 cctgagcgtc tcctcaaatc cggttgtcat gacagccttc cctgcttcaa gttttatacc
     1021 actggaagga tatattacct catggccgca tgttacaaaa cttctcaaaa gagtatgaag
     1081 cattgctttt ccctcaagca tgaattcctc gatgcccgca cccacagcat actctgccaa
     1141 aagtattttc atagatgaat gtaatggatt gataaatatt aaaactgcgg tttcttcctg
     1201 aaataccgct ctttctcact gaagatgcat ccgcaatatt tttgcatata aagtccgaga
     1261 gtctttgata tatcacggga ttcctgatat ccttccctga aatcctcata atagaactct
     1321 atcccggatt cacctgctat ttttttccct accgtgacaa ttgtctcatg tttctggtat
     1381 ggagaaatga gaagtgtggt cgtaaaagca tcaaaatcca gctgtcctgc aattcttgca
     1441 gtcttttcaa gtctctcttt gtagcagaat tcgcatcgtt cttctgcgac gagcgctcct
     1501 cgcagatagt tttctatatc gtaatcatta tcgtaaatta ctcttgttcc ttttaattcc
     1561 acatattttt caacagagac caggcgattt ttatattcct ggtagggatg gatattagga
     1621 ttataaaaaa aagcggttac atcatgccct tcctccagca ttcttttgtg cgggtatgtg
     1681 aaacatgggg cgcagcaaac atgggtgaga attttcatgt atctattaca tggtttcctt
     1741 gttctttatt ttaatgccat atcaaacttc ttcccaaaac aggcaaaact atatattgga
     1801 gttcaccgtt aacagcgtta gttaactctt aagtagaaaa ttaatcttga cttttataaa
     1861 gttgtgtttg tagctcttaa ttgttttaaa tataaattaa agaggttaga aagaaagagc
     1921 aattacgctt tttcataaaa attatggaaa gttttaaaat tctgggtata tgtgagcctg
     1981 ttttgagatc aataaaagat gagaagtttg aaagtcctag cgaaatccag gaaaaatcga
     2041 ttcctttgat cattgcaggg aaagatatta ttgccgggtc tgcaacggga tcgggaaaga
     2101 cgctagcatt tgcgtccggt atattgaaaa ataccgagaa aggaaaaggt attcaggccc
     2161 tggtcctgac tcctacaaga gaacttgctg agcaggtcgc ccaggcttta gcaaaatttt
     2221 caaagtataa acccctggaa attattgcgg tctatggcgg cgtgggaatt aatccccaga
     2281 tcaggggatt gaagactgca gacgttgttg taggaacacc aggcaggtta cttgaccata
     2341 tcgcaagaaa tactcttaga ttaggtaaag tgaaaacgct cgttcttgat gaagcagacc
     2401 ggatgttcga tatgggattc aaggatgatg tggaaaaaat aatcagaaaa tgccctgagg
     2461 aaaggcagac acttttattc tcagcaacca tcacaaaaga agttgtcctg ctttcaaagc
     2521 gttacatgca aaaaccgctt caggtttccg tagattcttt tgttgatcca aaaaaacttg
     2581 tacagacata ttatgatgtc gcagatgagc aaaaattctc tttgttattg catctgttaa
     2641 agaatgagag ctcagatctg gttatggtat tctgcaatac caagagaaat acggacaaag
     2701 taacaaaaaa cctgatattt tcaggtatag atgcccttgc tattcatggc ggtttgaccc
     2761 agtacaaacg aaatgatgta atgcagcgat tccataccgg aaaattctgt gtcctggtat
     2821 gcacagatgt ggctgcaaga gggcttgata tccgcggtgt ttcccacgtc tataattacg
     2881 atattcccag ggagagcaag cagtatatcc acaggatcgg aaggactgca agagcaggag
     2941 cagaaggaaa agccataaac atcctttcaa gaactgatta tgacaatttt ggaagggtat
     3001 tgaaagaaaa tgatgtggag ataacagaag agattttgcc tgcttttgag aaagctgcga
     3061 taagaaagcc tgaaagaagg gcaaccaagc ctattattag aaaagcacag ccacataaca
     3121 ggaacaggag gcgttaggaa atttccttca aatatttttc atggttcaat ccccattttt
     3181 agccctgtag ccgttcaaac cccatatatc aggccatata taaaaaaaga atcagtttga
     3241 tgctaaaaaa cgaaataaaa caatcatgaa tcttttttaa ctcaaaaaac tcaataaatc
     3301 gtcgatatat ttaaattatg aaatgatatt tggatgattt atgggtcgca aaattatagg
     3361 aaaaaatcca gagcgatgga tgaaaaaaaa tgctcatgat gatagtaata atatatatct
     3421 aaatgtataa ttaaagatgt atttatatgc taatgcattg ta
//
LOCUS       DATGBN010000250         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_408612, whole genome shotgun sequence.
ACCESSION   DATGBN010000250 DATGBN010000000
VERSION     DATGBN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408612"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="sepS"
                     /locus_tag="VKL21_06045"
     CDS             complement(<1..1403)
                     /gene="sepS"
                     /locus_tag="VKL21_06045"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HMB45364.1"
                     /translation="MKFNPDEIKEATKRDFDAAWNDGKKYVPNPGINEKYPRTSLKFG
                     KPHPVFDTIQRLREAYMRMGFEEFMNPIIVEDRDIHKQFGYEALAVLERCFYIGGLPR
                     PNVGISDERIVEIKNILGDIGDEGVEKIRLILHSYKKGEIEGDDLVPEMAAGLKVPDS
                     RVAVMIDHVFPEFKALVPISSQKTLRSHMTSGWFISLGEMGDYRKVPLKLFSVDRVFR
                     REQAEDATRLMAYYSASCVMMDENVSVEDGKAVASGLLSQFGFSNFTFRPDEKRSKYY
                     VPDTQTEVFAYHPRLVGSKTKYKDGWVEVATFGIYSPTALSQYNIPYPVMNLGMGVER
                     LAMILHDSTDVRAMTYPQFQYRTGWVMSDSEIAAMVYVEDTPLTETGKEIQTAIVRTC
                     EQYGNTTSPCEFTAWEGELFGRNILVMVVEPEENTRLCGPAAMNEVISYRNDILGLPR
                     ISKWDEAFKNGVSTGIR"
     gene            complement(1393..2505)
                     /gene="aroC"
                     /locus_tag="VKL21_06050"
     CDS             complement(1393..2505)
                     /gene="aroC"
                     /locus_tag="VKL21_06050"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMB45365.1"
                     /translation="MSGNTFGTLFRITTWGESHGPAVGVIVDGCPAGLLLDGSEIQKE
                     LNRRRPGQSDITTPRNEEDRAEILSGVFSGKTTGAPISILVKNNDVDSSKYEALRNTP
                     RPGHADMTYVLKYGFCDWRGGGRSSARETIGRVAAGAIAKKILGMNGINILGHVIELG
                     GIRANCMDIEQIHNNTEKNPVRCADPDAALQMEAMIHAARSQGDSVGGIVEVICTGVP
                     AGVGEPVFDKLNAELAKGLMSIGAVKGVEIGLGFKSSIIKGSQMNDPLEIRDGKPKPL
                     TNNAGGILGGISNGEPIVCRIAVKPTPSISKEQRTVDLSSMTDTQIKIIGRHDPSIPP
                     RIVPVAEAMVALVLIDMMMRGGFLKTSIMEKTIHEI"
     gene            2547..2753
                     /locus_tag="VKL21_06055"
     CDS             2547..2753
                     /locus_tag="VKL21_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45366.1"
                     /translation="MIDIFIDAEKCTGCGECIRVCPKGPRIYRLVKKNGKLVSEVLDK
                     SFCIGCTTCVASCRPKAIRLERGW"
     gene            complement(2775..3272)
                     /locus_tag="VKL21_06060"
     CDS             complement(2775..3272)
                     /locus_tag="VKL21_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HMB45367.1"
                     /translation="MKSKKEKIETHKQENIPIITSQMMEKTAIEIAQRKAGKKRSPIV
                     GVKHIDCPSCGTASMSFLDDLVFEVILAGERMVIPNLTGLKCTKCNEQAFDSKSTKII
                     DKYTSNKPLGGYECNISTVGGGKIGIYFPRDIIRVMKIKKNEKAVLTPLSSHKMIVEL
                     VELNA"
     gene            complement(3320..3637)
                     /locus_tag="VKL21_06065"
     CDS             complement(3320..3637)
                     /locus_tag="VKL21_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45368.1"
                     /translation="MRCQKNSSKRSYSDLVELKTVVMELVLNEKYVILWNHIKQYHVN
                     ITETEILNGLYYGLFKPDKNIESRFVSWSKFTISKRLIRIVFEVHEASDNDFVLVITA
                     FDE"
BASE COUNT          931 a          909 c          722 g         1180 t
ORIGIN      
        1 tatcttatcc cggtgctcac accattcttg aatgcttcat cccattttga gatcctgggc
       61 aatccaagga tatcgttcct gtaagaaatc acctcattca tcgcagccgg tccgcaaagc
      121 ctggtattct cctcaggctc aactaccatg accaggatat tccttccaaa caactctcct
      181 tcccatgccg taaactcaca tgggcttgtt gtattcccgt actgctcgca agtcctgact
      241 atcgctgttt gtatctcttt tccggtttct gtcaacgggg tgtcttcaac atataccatt
      301 gctgctattt cgctatcgga cattacccag ccggttctgt actggaactg gggatatgtc
      361 atggcgcgca catctgtaga atcgtgcagt atcattgcga gacgttccac acccattcca
      421 aggttcatca caggatatgg gatattgtac tgtgagagag cagttggcga ataaatcccg
      481 aaagttgcca cttcaaccca gccgtccttg tattttgtct ttgaacccac aagcctgggg
      541 tgataggcaa aaacctcggt ctgggtatca ggcacataat atttgcttct cttttcatca
      601 ggcctgaaag tgaaatttga aaacccgaat tgggaaagaa gaccggaagc cacagccttt
      661 ccatcttcca cgcttacatt ctcatccatc ataacgcatg atgcggaata ataagccata
      721 aggcgcgtgg catcttcagc ctgctcgcgc ctgaataccc tgtctacgga aaatagctta
      781 agaggcactt tgcggtaatc gcccatctca cccaggctta tgaaccagcc tgaggtcata
      841 tgactgcgga gtgttttctg cgaggaaatt ggaacaagtg ccttgaattc agggaacaca
      901 tggtctatca tcactgcaac cctggaatcc ggaactttta atcctgctgc catttcagga
      961 acgaggtcat ctccttcaat ctcacctttt ttgtatgagt gaagaatgag tcttatcttt
     1021 tccacacctt catcaccaat atctcccagg atatttttta tctcaacgat cctttcatcc
     1081 gatatcccca cattgggtct tggaagcccg cctatgtaaa aacacctttc aagcactgca
     1141 agggcttcat atccgaactg cttatggata tccctgtctt ccactattat cgggttcatg
     1201 aattcttcaa atcccattct catataggct tcacgcaggc gctgtatggt atcaaagaca
     1261 ggatggggtt taccgaattt tagcgaggta cgcggatatt tttcattgat gcccgggttt
     1321 ggaacatatt tttttccatc gttccatgcg gcatcaaaat ctctttttgt tgcttctttt
     1381 atctcatcag gattaaattt catgaatagt tttctccatg attgaggttt tcaagaaccc
     1441 gcctctcatc atcatatcta tcagcacgag cgccaccatt gcttcagcca ccggaactat
     1501 tcgcggcggg atggatggat catgccggcc aattatcttt atttgtgtat ctgtcattga
     1561 gctaaggtca actgtgcgct gttctttaga tattgacggc gtgggtttaa ctgcgatcct
     1621 gcaaacgata ggttcaccgt tgctgatgcc tccaagaatt cctccagcat tatttgtgag
     1681 aggctttggc ttgccgtccc ttatctccag aggatcattc atctggctgc cttttattat
     1741 tgaactctta aaccccaggc ctatctccac gcctttcacc gcgccgatgc tcattaatcc
     1801 ctttgcaagt tccgcgttaa gcttatcaaa aaccggctca cctaccccgg cagggactcc
     1861 ggtgcatata acttcgacta ttcctcccac actatctccc tggcttcttg cagcatggat
     1921 cattgcttcc atttgcaggg ctgcatccgg atcagcacac ctcactggat ttttttccgt
     1981 gttattgtgg atctgttcga tatccatgca atttgccctg atacctccga gttcaattac
     2041 atgacccaat atatttatgc cattcattcc aaggatcttc tttgcaattg cacctgctgc
     2101 gacacgtcct attgtttcac gcgcggatga cctgccccct ccgcgccagt cacagaaccc
     2161 atattttaat acatacgtca tatccgcatg tccggggcgg ggagtgttgc ggagagcttc
     2221 gtatttgctc gaatcaacat cattattttt cacaagtatt gagatgggcg cacctgtggt
     2281 ctttcctgaa aatacccctg acaatatctc tgctctatcc tcttcgttac gcggtgttgt
     2341 tatatcgctt tgtccgggac gcctgcggtt caattctttc tggatttcag atccgtcaag
     2401 caaaagaccg gcagggcagc cgtcaacgat tacaccaacg gcagggccgt ggctctcacc
     2461 ccaggtcgtt attcgaaaaa gtgtgccaaa ggtatttcca gacatgcgct ttaatttagt
     2521 atgattgctt atataactgc ttggacatga tagatatatt cattgatgct gaaaaatgca
     2581 ccggatgcgg ggaatgcata agggtttgcc cgaaagggcc aagaatatac aggctcgtaa
     2641 agaaaaatgg aaagctggta tccgaagtcc tggataaaag tttctgcatc ggatgcacaa
     2701 catgtgtagc atcgtgcagg ccaaaggcta taagactaga gcgtgggtgg taaaaatttt
     2761 cacggtcccg tttttcatgc attcaactca accaattcaa cgatcatttt atgactggat
     2821 aacggtgtca agacagcttt ttcgttcttt ttgatcttca taacacgaat aatatccctt
     2881 ggaaaatata ttccgatctt cccaccacct acagtagaaa tattgcattc gtacccaccc
     2941 aacggcttat ttgatgtata tttatcgatg atctttgtgg attttgaatc aaaagcctgt
     3001 tcattgcatt tcgtgcattt aaggcccgtg agatttggta tgaccatcct ctcgccagca
     3061 aggataactt caaagaccaa gtcgtctaag aagctcattg aagctgtccc gcatgaagga
     3121 caatcgatat gttttacacc aacaattggc gacctttttt tccctgcttt tcgttgagca
     3181 atctcgattg cggttttttc catcatttgc gaagttataa tcggtatatt ttcctgttta
     3241 tgagtttcga ttttttcttt tttactcttc atctggattc tccttaattt atgtgaaacg
     3301 gtcaaacatt atggattttt cattcatcaa atgctgttat cacaagtaca aaatcattat
     3361 ctgaagcttc atggacttcg aatacaattc gtataagccg tttggatata gtgaatttag
     3421 accatgatac aaatcttgat tcgatattct tgtcaggctt gaataatccg taatatagtc
     3481 catttagaat ctccgtctca gtgatattca catgatactg cttaatatga ttccataata
     3541 tgacatattt ttcattcaat accagctcca tgacaactgt tttcaattct acaagatcgc
     3601 tgtatgatct tttcgatgaa tttttttgac atcgcattca ggtctgctcc atatacagaa
     3661 tctattttca tgttgttcgt atcccgtatc atttttatgt atttacgtat aatatactta
     3721 tattttacgt ttactccaga ta
//
LOCUS       DATGBN010000251        10698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_485988, whole genome shotgun sequence.
ACCESSION   DATGBN010000251 DATGBN010000000
VERSION     DATGBN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485988"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2441)
                     /locus_tag="VKL21_06070"
     CDS             complement(<1..2441)
                     /locus_tag="VKL21_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HMB45369.1"
                     /translation="MWEKKGLYIIINKVLPGLSRRLPVLVICLLFIQFMCVSALAQKV
                     EYHDGYNLISYDDGSKDIINGDLNFLRWDGVWRPNDELNISNGSWPYLYSGNATTADF
                     RVDDTTLTIPRANTQFKLKPNSISYNIVYSKSELLGKETSINLTDAFIYFPYNLKSRT
                     LKNGYKDKYNIKYGNFNFKAGMEHIAVHDDTPRNYIEDGKVFQDVTYLFPPDYEFKID
                     NGEIRLVFNKNSLNKLKGNVIIEIRTWNIIDTNHWGGNVTFSQTTDVKATGNVELKQM
                     VDDYTLYTRFDEGNGTIIHNENTLNRPLGDLLGVVGSGTYTTGKYGQAIHFNGIDNKV
                     LFNDHPDFRLPGDFTISYYIKLTSDVSNVDSDITRKGSTATANPKSWWKIELTNNLMH
                     GSITKDGATAVESYDTVDRRDGLWHFVAYNRGGTTCSLIVDGVTIKANTCTTNAVNTA
                     LLSIGAKDTYVQTTGLDFTHGTIDEVRIYNRKLSASELTSIRNNAHYTAGTVTRNLVS
                     IIQAGEEIKELGCYGTWDSSITKVDIMASADNSIWNTIKSNATPNVNYAVNSGNNYKY
                     SRCSLSTTDSSKSPIIQSIRANIGLKSASFNGTNAPILSSVSISSNNANSSKAKVGNV
                     VNVTFTANETLLTTPIATIAGQSATVTSTGGNSYNASFTMTDSQVNGAVAFTIDFSDA
                     AGNPGTQVTAVTDSSSVTFDRSAQMLSSVSISSNNANSSKAKVGNVVNVTFTANETLL
                     TIPTVTIAGHSVTVASTGGNSYYATYTMVSNDMEGAVSFTINFTDAAGNDGTQVTLTT
                     DESSVTFYKTSPAVI"
     gene            complement(2585..3247)
                     /locus_tag="VKL21_06075"
     CDS             complement(2585..3247)
                     /locus_tag="VKL21_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45370.1"
                     /translation="MNLDECRAICTSPQIPTRKKLFFRIIYEPQLRPFEALNLLIENW
                     DREQKLVTAVRVKQKTAPVKGDRHRKMRLPSKPKTAFITENTNLMLKTVVSNCKKRSI
                     FVNANGDKLSIAWFKDRINHYLKLLGIQKDVKYYDTGTGEVHPRDLITCMALRETGEQ
                     HHDNAGGPGNYRRLLQGTLCSLIKMKKKATTLIFKSNDVFQKSYTIPWLGNKRLRFDW
                     IS"
     gene            complement(3362..4375)
                     /locus_tag="VKL21_06080"
     CDS             complement(3362..4375)
                     /locus_tag="VKL21_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMB45371.1"
                     /translation="MMLKKSNKSALLILILVLLLLFLITFIYLKNSTVIDKFPNVTGK
                     QPEPVTTTSQELKSIPSQITSSTIITSDYETIKVGAFNIKMFGTTKAGKPEVMEVLSK
                     IIRNYDIIAIQEIRGDSQTALPALIYTVNSAGNPQYNFIVSESLGETKHTARSKERYA
                     YFYNTQTIQLTGSSYKYPNSNALFQREPYVSEFKAKNGNFDFVLITIHIDPDNATQEI
                     NEMPKVVEDARNRYQGESDFIIIGDLNADCSYFNENSPSSLQTSDYYWIINNSVDTTT
                     IEPINCTYDRIIITTSSLTDFTGESGVFRFDTAYNLSYISTMAVSDHYPVYANFWSNR
                     DTD"
     gene            complement(4410..4712)
                     /locus_tag="VKL21_06085"
     CDS             complement(4410..4712)
                     /locus_tag="VKL21_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45372.1"
                     /translation="MGSSLFGGYDREISICGYWVLRRLPGTDMPYISGRVPDHLELIL
                     QSTTNKDILNITAYYIIKDAKINKTELYIKPLKEFVLKYETRTIHFDAGQNHQIKF"
     gene            complement(5024..6613)
                     /locus_tag="VKL21_06090"
     CDS             complement(5024..6613)
                     /locus_tag="VKL21_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HMB45373.1"
                     /translation="MSKISILGIEGTAWNLSAAIVDEKDVIAETTSTYRPTAGGIHPR
                     EAAQHHANNITKVVSETIKKAGRKVDAVAFSQGPGLGPCLRTVATAARALALSLDVPL
                     IGVNHCIAHIEVGRWKTPAKDPVTLYVSGANSQVLAYRKGHYRVFGETLDIGIGNALD
                     KFARSVGLAHPGGPKVEELAKQATQYIPLPYTVKGMDFSFSGLTTAAQESATKFALHD
                     VCHSFQETAFAMLLEVTERAVAHTGKGEVLLAGGVGANSRLCEMLAIMCEDRGIDFYV
                     PEKRFMGDNGAMIAYLGLLMYKSGYRTSIPESKVNPNFRADEAVVRWNPEEGRMFKEE
                     MPGTHGKIASGAEAVITLSGNTITKTRISKRYRLKEMDETLRKERTRIEARLISEARR
                     LGVPTPIIRDVSDFELKMEYIMGIALKKVITPSLSEHTGELVGRLHTHGIIHGDLTTS
                     NILFKDGKLYLIDFGLSYLDKTVEARGVDVHVLFQTFESTHENHEELIETFKKGYALT
                     FAGAEEVLGREREIEARGRYA"
     gene            6745..7062
                     /gene="eif1A"
                     /locus_tag="VKL21_06095"
     CDS             6745..7062
                     /gene="eif1A"
                     /locus_tag="VKL21_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HMB45374.1"
                     /translation="MLNRRTNNSQQPEEFTRVRLPQKKAGEVLGTVESLLGANKLRVR
                     CMDGVVRLTRIPGKMKKSTWIREGDVVIVVPWSFQTEKADIIWKYSGPQVNWLERKGF
                     LKS"
     gene            7072..7872
                     /locus_tag="VKL21_06100"
     CDS             7072..7872
                     /locus_tag="VKL21_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HMB45375.1"
                     /translation="MASDKKLSRMDEKVDEFRMRLKDSDQKQVLNDVFDTATLLALYD
                     LSKKGYIDALGGCFSTGKEANLFHAVANKDDISEVAVKIYLISTANFNSMKDYILGDP
                     RFQGIRHARKDIILAWTKKEFKNLKRAEEAGVRVPKPYITQRNILLMEFIGKDGIPMP
                     QLKDVNLSTKEASHIFDLLVDYMNRLYSRAKLVHADLSEYNILVDMNKMEPVIIDMGQ
                     SVLTSHFNAETYLRRDVANIARFFGKLNVEANEDNMISIIKKNKEEEQ"
     gene            7869..8405
                     /locus_tag="VKL21_06105"
     CDS             7869..8405
                     /locus_tag="VKL21_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HMB45376.1"
                     /translation="MTEHIKIPLERIAVLVGPKGQTKGLIEEKSTAILNIDSESGNVE
                     IANPTDALMGMRAKEVVQAIARGFSPEKALKLFDHDLLMFETIDLSNIARTEKDLMRI
                     RGRLIGKDGKTREIFETLTNAYISVYGKTVSLIGYPEQNTVARTGINMLLEGSAHGPV
                     YSFLEKKKSDLKRAELGL"
     gene            8509..8883
                     /locus_tag="VKL21_06110"
     CDS             8509..8883
                     /locus_tag="VKL21_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator protein"
                     /protein_id="HMB45377.1"
                     /translation="MKALKIKQLDEKDEEIADILISLGMSRNVAMSLTYLQNMNSATS
                     VDLERVARLRQPEVSIAMRQLKERDWIDEREEKTPGKGRPYKVYSLKVGFKDIVSQLE
                     KQQRKVVDEAKANVERLKELGK"
     gene            complement(8912..9754)
                     /locus_tag="VKL21_06115"
     CDS             complement(8912..9754)
                     /locus_tag="VKL21_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45378.1"
                     /translation="MRNIIRKIENGSAGESNISSLCELLEDHPDMIQESIGVLNAILI
                     KGDIKAYNSAISALNKLAEKYVGFEHFSVDVIVNCMQERKNDLHENGLLKILEILSKI
                     TQKSPERMKIAVPELFICLENTNPKSREIAHFILSIIAAAHHEFFKGRSKEVIRVLNG
                     LYVDERIYACRLLKQLAQNDQTRVDDTHDLLEDLRLNHPDCNLRSEAGFAMDKLKEAI
                     RKNSSGVDRPIKLIKPEPSALVKDRKVISDDFSGLSELIAPNKKDIIDLLEGMNLRHM
                     IINR"
     gene            complement(9874..10233)
                     /locus_tag="VKL21_06120"
     CDS             complement(9874..10233)
                     /locus_tag="VKL21_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HMB45379.1"
                     /translation="MRKAGDVMKKIEAIIRSEKLGDIKNALAIAGFIGLTTYEVKGRG
                     RQKGVVLQYRTQEYRVDMLAKTKIELVVNDTDVEKVIEIICESGRTGNIGDGKIFILP
                     IEEIIRVRTGERGREAI"
     gene            complement(10249..>10698)
                     /locus_tag="VKL21_06125"
     CDS             complement(10249..>10698)
                     /locus_tag="VKL21_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMB45380.1"
                     /translation="ALGIVTGGVAGLAAITPASGYVGPLWAIIIGGAAGVICYFVMHL
                     KNKKTMIDDSLDVFACHGVGSAWGVIATGIFASVAINPAGVNGLVFGGYALIKSQFIA
                     VVVTGIYSFVMTVIILKILNATMGLRVRDDEEIEGLDITQHGEKAYV"
BASE COUNT         2980 a         2215 c         2112 g         3391 t
ORIGIN      
        1 tttatgactg caggtgaagt cttgtagaag gtgactgaac tttcatcagt tgtcaaagtt
       61 acctgcgttc cgtcattgcc cgctgcatca gtgaagttta tcgtgaacga tactgctcct
      121 tccatatcat tgctgaccat agtgtatgtt gcatagtatg agtttcctcc tgtgctggct
      181 actgtgacgg aatgaccggc aatagtcacg gttggaatgg tcaatagggt ttcgttcgcg
      241 gtgaaagtta cattgacgac gttacctact ttggcttttg atgagtttgc attgttcgat
      301 gagatcgata ctgatgagag catctgagca ctcctatcaa aagtgactga acttgaatca
      361 gttactgcag tcacctgtgt tcctggattg cctgctgcat cactgaagtc gatagtgaat
      421 gcgacggctc cattcacctg ggaatcggtc atggtgaaac tggcattgta tgagttacct
      481 cctgtgctgg ttactgtggc tgactgcccg gcaatggttg ctattggagt ggtcaataac
      541 gtttcgttcg ctgtgaaggt tacattgacg acgttaccta ctttggcttt cgatgagttt
      601 gcgttgttcg atgagatcga tactgatgag agtatcggag catttgtacc gttaaaactt
      661 gcagatttta gacctatatt tgctctgata ctttgaatta tcggggactt tgaagagtca
      721 gttgtactca gcgaacatct tgaatattta taattgttac ctgaattaac agcataatta
      781 acatttggag ttgcatttga ttttatcgta ttccaaatgc tattatcggc acttgccata
      841 atatccactt tcgttattga actatcccat gttccatagc atcctaactc ttttatttcc
      901 tcccctgcct ggataataga aacaaggtta cgagtgactg tacctgctgt ataatgtgca
      961 ttattccgaa tagatgtaag ttccgaagct gataatttac gattatatat tcttacctca
     1021 tctatggttc catgggtgaa atccaatcct gtggtttgca cataggtatc ctttgcccct
     1081 atgctcaata aagcagtgtt tacggcattt gtcgtgcatg tatttgcttt tatcgtaacc
     1141 ccgtctacta tcaaactgca tgtggtccct cctctgttat atgcaacaaa atgccacaat
     1201 ccatccctgc gatccactgt atcgtacgac tcaactgctg tagcaccatc ttttgttata
     1261 gagccatgca ttaaattatt tgttagctca attttccacc atgatttagg attcgcagtg
     1321 gcagtgctac cttttcttgt tatatctgaa tcaacattgc tcacatctga agttaattta
     1381 atgtaatatg agatcgtaaa atcacccggc aaccggaagt ccgggtgatc attgaacaaa
     1441 actttattat ctatcccgtt aaaatgaata gcttgcccat attttccagt tgtataggtt
     1501 ccagatccca caacacctaa aagatcaccc aggggtctgt ttaaggtatt ttcattatgt
     1561 attattgtcc catttccttc atcgaatcta gtgtataatg tgtaatcatc aaccatctgt
     1621 ttgagttcca cattaccagt agctttaacg tctgtagttt gagaaaatgt tacatttccg
     1681 ccccaatgat tagtgtcaat tatgttccat gttcttatct caataatgac atttcccttc
     1741 aatttattta gcgagttttt gttaaaaacc aatcgaattt caccattatc tatcttaaat
     1801 tcgtaatcag gtgggaaaag atatgttacg tcctggaata cttttccatc ctctatataa
     1861 ttcctgggcg tatcgtcatg taccgctata tgttccatcc ctgccttaaa attaaaattg
     1921 ccgtatttaa tattatattt atctttatat ccatttttaa gcgtcctgga tttcaaatta
     1981 taaggaaaat atataaatgc gtccgttaga ttgatggatg tttctttccc caacaattct
     2041 gattttgaat atactatatt ataactgatc gaatttggtt tcaatttaaa ttgtgtattt
     2101 gctcttggaa tagtcagagt ggtatcatct accctgaaat cagcagtcgt tgcatttcca
     2161 gaatataaat aaggccagct tccattggaa atatttaatt catcgtttgg tctccataca
     2221 ccatcccatc taagaaaatt caaatctcca tttattatat cctttgaccc atcgtcgtaa
     2281 gagattaaat tatatccatc gtgatattcc actttttgcg cgagggcaga tacacacatg
     2341 aattgaataa ataaaagaca tattacaagt acaggtaatc gccttgaaag accaggcaat
     2401 actttattga taatgatata cagacctttt ttttcccaca taatcatacc aatttttatt
     2461 tagattgcca atagagcaca ttattattta tatgtttgta tggttttgtt attataatta
     2521 ttgctattat tatcattaat caccgatttt cgtcaagtgt ttatgtctca taattgaacc
     2581 ctggtcagga tatccaatca aagcgaagtc tcttgttgcc taaccagggt atggtatacg
     2641 atttctgaaa aacatcattg gatttgaaaa taagtgttgt tgctttcttc ttcattttga
     2701 ttaacgagca tagtgtgccc tgcagcagcc gccgataatt tccgggacca cctgcattgt
     2761 catgatgctg ttccccagtc tccctgagag ccatacaggt aatgagatcc ctgggatgta
     2821 cttctccagt cccggtatcg taatacttca catctttctg tattccaagc agcttcaaat
     2881 aatggtttat cctgtccttg aaccatgcta ttgaaagctt atcgccgtta gcatttacga
     2941 agatggatct tttcttgcag ttgctaacaa ccgttttcag catgaggttg gtattctcgg
     3001 taataaaagc tgtcttgggc tttgaaggaa ggcgcatctt cctatgcctg tcacccttaa
     3061 caggcgcagt cttttgcttc accctgaccg ccgtaactaa tttctgttca cggtcccagt
     3121 tctctatcag aaggttcagg gcctcaaaag gccgcagctg cggttcatag atgatcctga
     3181 agaacagctt ttttctggta ggtatctgag gtgaggtgca gattgccctg cactcatcaa
     3241 ggttcatccc tctgtgggta tttttgggcg acgcacccaa aaggtaaaat aagtgctgtc
     3301 tggtgacacg aacgtttgta ctaccccaag atgaagaggg ctttaatgtt tttatgcata
     3361 ttcaatctgt atccctgttg ctccagaaat tagcataaac aggataatgg tcagaaaccg
     3421 ccattgtgct aatataactc aagttataag ctgtatcaaa cctgaacact cctgactccc
     3481 ctgtaaagtc ggttaatgaa gaggttgtta ttataattct gtcgtatgtg cagttaatcg
     3541 gttctattgt ggttgtgtca acgctattgt taataatcca ataataatca ctggtttgga
     3601 gtgaagacgg actattctca ttgaaataac tgcaatcggc attcaaatcc cctattataa
     3661 taaaatcact ttctccctga tacctgttcc tggcatcctc aaccacctta ggcatttcgt
     3721 taatttcttg agtggcatta tcggggtcta tgtggatagt tatcagcacg aagtcaaagt
     3781 tgccattctt ggctttgaat tctgagacat agggttcgcg ttggaaaagg gcattcgagt
     3841 ttggatattt ataagaagat cctgttagct ggattgtctg tgtattgtag aaataggcat
     3901 aacgctcctt gcttctagct gtatgcttag tctcccccag ggattcactt acaataaaat
     3961 tatactgagg gtttcctgct gaatttaccg tgtatatgag agcaggcaag gctgtttgtg
     4021 aatcgccacg aatctcctgt attgcaataa tgtcatagtt cctgatgatt ttggaaagga
     4081 cttccattac ttcaggttta cctgccttag ttgtgccaaa catttttata ttgaaggcac
     4141 ctactttgat tgtttcataa tcactggtta tgattgttga tgatgttatt tgtgatggta
     4201 ttgattttag ttcttgagac gttgttgtta ctggttcagg ctgtttacct gttacatttg
     4261 gaaatttatc gattactgtc gagtttttaa ggtagatgaa tgtgattaaa aatagtaaca
     4321 agagtactag aataagaatc aataaagctg atttattaga tttcttcaac atcataatat
     4381 tcaagtctga catcatagat tactggattt taaaatttaa tctggtgatt ttgtccagcg
     4441 tcaaagtgta ttgttcttgt ttcatacttc aggacaaact ccttcagcgg ctttatgtat
     4501 aattctgtct tatttatttt agcgtcttta ataatataat atgccgttat gttgagtata
     4561 tctttattgg ttgtgctctg caatatcagc tccaggtgat ccgggactct tccgcttata
     4621 tagggcatat ctgtaccagg cagtcgcctg agtacccaat atccacagat acttatttct
     4681 cgatcatatc ctccaaataa agaactgccc aaagtccccc caacgttctc tacgtttttt
     4741 tcctgaaaaa aattggtacg catttactta cggaggagct cgattcatag atcttcaggt
     4801 tgaaaatgag aaatatatgg cactttgtat ttttacatag ctcaatatac tctacaatta
     4861 aattataaat cccaaacctt tatattaaga tgcaacttat cttaagtcga aggcttttgc
     4921 tgtggattat agtagccctc caggggctct tgtcattatt atgttctatt tgtcccaaca
     4981 caaagtaatc ccatgaaaat ctaagatgag catataaccc tcttcacgca taccttcccc
     5041 tcgcctcaat ttctctttct ctccccagga cttcctcagc gcctgcaaat gtcaaagcat
     5101 atcctttctt gaaagtctca ataagctctt catggttctc atgcgtactc tcgaatgtct
     5161 ggaaaagcac atgcacatca acccctctcg cttccactgt cttatcaaga tatgaaagcc
     5221 cgaaatcgat caggtagagt ttgccatcct tgaataagat attcgaagtg gtaagatccc
     5281 catgaattat tccatgcgta tgcaagcgac ctaccagttc tcccgtgtgt tcactcagcg
     5341 aaggagtgat cacttttttc agggctatcc ccattatgta ttccattttt aattcaaaat
     5401 cagaaacatc cctgatgatt ggcgtaggaa cacccaagcg gcgcgcttct gaaatcagtc
     5461 tggcttcaat tcgtgtcctt tctttacgta aggtttcatc catttctttg aggcggtacc
     5521 gtttcgatat cctcgttttg gtgatcgtat ttccggaaag agttatcacg gcttctgctc
     5581 cgcttgcgat ttttccatga gtgccgggca tttcttcctt gaacatcctt ccttcttcag
     5641 gattccatct aactactgct tcatccgctc tgaagttggg attgaccttt gactcgggaa
     5701 tcgaagtcct gtatcccgat ttatacatga ggaggccaag ataagcgatc attgcgccgt
     5761 tatcccccat gaaacgcttt tcaggcacat agaaatcaat gcccctgtcc tcacacatga
     5821 tagcaagcat ctcacacagg cgtgaattag ctccaactcc gcctgccagc aggacttcgc
     5881 cttttcctgt atgcgcaacg gcgcgttccg ttacttccag aagcattgca aaagcggttt
     5941 cctggaaaga atggcataca tcgtgtaaag cgaattttgt tgccgattcc tgcgctgctg
     6001 tggtcaatcc tgagaatgaa aaatccatgc cttttacagt atagggcagc gggatgtatt
     6061 gtgtagcctg ttttgctagt tcttctactt tcggtcctcc gggatgcgct aatcccaccg
     6121 accgtgcgaa tttgtcaagg gcgttgccga taccgatatc aagcgtctct ccgaataccc
     6181 tgtaatggcc tttcctgtat gccaggacct gcgagttcgc accgcttaca tataaagtca
     6241 cagggtcttt tgccggtgtt ttccatcttc ccacttcaat atgcgcaatg cagtggttca
     6301 cgccgatgag aggaacatca agtgataatg caagagccct cgcggccgta gcaaccgtac
     6361 gaagacacgg accaagcccc gggccctggg aaaatgccac tgcatccacc tttcgccccg
     6421 ctttttttat ggtttcactt acaacttttg ttatattatt ggcatgatgc tgcgctgcct
     6481 cacggggatg gatgccgcct gctgtggggc ggtaagtaga tgttgtttct gcaataacat
     6541 ccttttcatc aactattgct gcgctcagat tccatgcagt tccctcaatt ccaagaatag
     6601 agattttaga catgatttta cattggcttc attacatttg gttctaaaat aagtatcgga
     6661 taattgtatt tattttttat ataaataatt gtcagtaacc tttacatata gtgtggacta
     6721 tattgatata aatcatgagg tgttatactg aataggcgaa ccaataatag ccaacagccg
     6781 gaagaattta caagagttcg cctgccgcag aaaaaggcgg gggaagtgct ggggactgtt
     6841 gaaagtcttt tgggggcaaa taaattgcga gtacggtgca tggacggcgt agtgagacta
     6901 actcgcattc cgggaaaaat gaaaaaaagt acatggatcc gggaagggga tgttgttata
     6961 gttgtaccat ggagtttcca gacagaaaaa gccgatatta tctggaaata cagcggccca
     7021 caggtgaact ggctggagag aaaaggattt ttaaaaagtt aaattacgtg tatggccagt
     7081 gataagaaac tttcccgcat ggatgagaaa gtcgatgaat tccgcatgcg tttaaaggat
     7141 tccgatcaga agcaagttct taatgatgta tttgacacag ctacattgtt ggctctttat
     7201 gacctttcaa aaaaaggtta tatcgatgcg ctggggggtt gttttagtac aggtaaagaa
     7261 gcaaatcttt tccacgctgt agcaaataaa gacgatatat ccgaagttgc tgtgaagata
     7321 taccttatat caacagcaaa tttcaattcg atgaaggatt acattctggg ggacccgcgt
     7381 ttccagggca taaggcatgc aagaaaagat attattcttg catggacaaa aaaggaattc
     7441 aagaatttaa aacgcgcaga agaagcagga gtaagggtgc caaaacccta tatcacgcaa
     7501 aggaacatat tgttaatgga gtttatagga aaagacggca tcccgatgcc tcaactaaag
     7561 gatgtaaacc tttccactaa ggaagcatca catatttttg acttgctggt cgattatatg
     7621 aaccggctat attccagggc aaaacttgtt catgcggatc taagtgaata taatatattg
     7681 gtcgacatga ataaaatgga acctgtaata attgatatgg gtcagtcggt cctgaccagt
     7741 catttcaatg cagagactta tcttcgtcgc gatgttgcca atatcgcacg atttttcggg
     7801 aaattaaatg tcgaggcaaa tgaagataat atgatttcga tcataaaaaa aaataaagag
     7861 gaagaacaat gacagagcat attaaaattc cgcttgaaag aatagcggta ctggtaggcc
     7921 ctaaaggcca aacaaaagga ctcattgagg aaaaatcaac tgcaatttta aatattgata
     7981 gcgagagcgg gaatgtggaa atagcaaacc cgacggatgc gcttatggga atgcgggcaa
     8041 aggaagtcgt acaggcaata gcaaggggtt ttagccctga aaaagccctg aaattgtttg
     8101 accatgacct tcttatgttt gaaacgatag acctttcaaa tatcgcacgg acagaaaaag
     8161 accttatgag gataagggga agactcatcg gaaaggatgg caagaccagg gagatatttg
     8221 aaacccttac aaacgcatat atctcggttt atggaaaaac ggtaagcttg atagggtatc
     8281 ctgagcagaa tacagtagcc aggacgggta tcaatatgct tcttgaaggt agcgcccacg
     8341 gccctgttta ttcgttcctt gagaagaaga agagcgattt gaagagagca gagttgggat
     8401 tgtaggattt cataagtcct ttgatatttt tctttttgca gagtccggat atttttctta
     8461 tcgaaagatt aaaataatac aagggcataa tatatttctg gagtcaatat gaaagcgctt
     8521 aagataaaac agctcgatga aaaagatgaa gaaattgcgg atatacttat ttccctggga
     8581 atgagcagaa atgtggcaat gagtcttacc taccttcaga atatgaattc agctacctca
     8641 gtggatcttg aaagagtggc aaggttacgc cagcccgaag tcagtattgc catgaggcag
     8701 ctaaaagaac gggattggat agatgaacgg gaagagaaga cgccagggaa aggaaggcca
     8761 tataaggtat actctctcaa agttggattc aaggatatcg tttcacagct agaaaaacag
     8821 caaagaaaag tagttgatga ggcaaaagca aatgttgagc gtttgaagga attagggaaa
     8881 tagtgttctt ttcctcaatt ttatgatatg gttatcgatt gataatcata tgcctgagat
     8941 tcattccttc aagaaggtca attatatctt ttttatttgg cgcaatgagt tcagataatc
     9001 cggaaaaatc atcagaaatt actttcctat ctttaacaag tgcgctgggc tccggtttta
     9061 ttaactttat tggccgatcc actccggagg aattctttct gatagcttct tttagcttgt
     9121 ccatcgcgaa tccagcctct gaacgtaaat tacaatccgg atggttaagt cgcaagtctt
     9181 ctaatagatc gtgcgtatca tcgactcgtg tttgatcatt ttgtgcaagt tgttttaata
     9241 acctgcatgc atatattctt tcatcaacgt acagaccatt tagaacccga atgacctcct
     9301 tcgagcgacc tttgaaaaac tcatgatggg cggctgcaat gatgctaagt atgaaatgtg
     9361 ctatctctct acttttcgga ttcgtatttt caagacagat aaacaattca ggtactgcaa
     9421 ttttcatcct ttccggagat ttttgagtga tttttgaaag aatctcgagt atctttagca
     9481 aaccgttttc atgaaggtca tttttccttt cctgcatgca gtttacaata acatctactg
     9541 aaaaatgttc aaacccgaca tatttttcag ccaatttatt caaggctgaa attgccgaat
     9601 tatatgcttt tatgtcacct ttgatcaata ttgcgttaag cacccctatg gattcctgta
     9661 tcatatccgg atgatcctca agcaattcac aaagagatga aatattggac tcacccgcgc
     9721 ttccattctc gattttccta attatatttc gcactttttt ttgaaaatta ttatagaaaa
     9781 acatgttata cctccaaatg tttcgaattt cacattttcc tacaatttaa tatttcaatg
     9841 aacatttata taaattctat ggacacaaaa tttctatatg gcttctcttc ctctttctcc
     9901 tgtccttacc cgaatgattt cttctatggg caggataaaa atcttgccgt cgccaatatt
     9961 acctgtcctt cccgattcac agattatttc tatgaccttt tcgacatccg tatcattcac
    10021 aacaagctca attttggtct tggcaagcat atccaccctg tattcctggg tccggtattg
    10081 cagcacaacc cctttttgcc ttccgcgtcc ttttacttcg tatgtggtta gaccgataaa
    10141 acctgctatt gccagcgcat tcttgatgtc tcccagcttt tccgacctga ttatcgcttc
    10201 aatttttttc atgacatcac cagcctttcg cataagtcat cctgcttttt aaacatatgc
    10261 tttctctcca tgctgggtaa tatcaagacc ttctatctct tcatcatccc ggacacgcag
    10321 acccatagtt gcatttagta ttttcagaat aatgacggtc atcacaaatg aataaatacc
    10381 ggtcacgaca actgcgataa attgggattt tattaaagca taaccgccga ataccaggcc
    10441 atttactccc gcaggattaa tcgcaactga agcaaagatc cctgttgcaa tgacacccca
    10501 ggcacttcca acaccgtggc aggcaaatac gtcaagtgaa tcgtctatca tggtcttctt
    10561 gttcttcaag tgcattacaa aatagcatat tactccggct gcgcctccta ttattatagc
    10621 ccaaagcggc cccacatatc ctgatgccgg tgtaattgcg gcaagtcctg cgactccccc
    10681 tgtgacaatt ccaagagc
//
LOCUS       DATGBN010000252         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_485989, whole genome shotgun sequence.
ACCESSION   DATGBN010000252 DATGBN010000000
VERSION     DATGBN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485989"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..779)
                     /gene="ilvN"
                     /locus_tag="VKL21_06130"
     CDS             complement(279..779)
                     /gene="ilvN"
                     /locus_tag="VKL21_06130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HMB45381.1"
                     /translation="MKHTLAILVENKPGVLTRVAGLFSRRGFNIESLAVGVTENLDTS
                     RITIVVTGDDNILEQVEKQLNKLIDVIRVSDIPAQDSVNRELALIKVGVDSTTRAEVM
                     QIVDIFRAKIVDVGIKSLVIEVTGDESKINAIEQLLRQFGIREMVRTGKIAMNRGAKV
                     VQSEKR"
     gene            complement(784..>1508)
                     /locus_tag="VKL21_06135"
     CDS             complement(784..>1508)
                     /locus_tag="VKL21_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMB45382.1"
                     /translation="VGDAKNVLKSLIKYVKIEQAKTDAWIKKINAWKKEYPLTYKKDN
                     LLRPQYVVEQISEICPDAIIVTEVGQNQMWAAQFFNYKNPRTFISSGGLGTMGYGFPA
                     SIGAKVGKPECTVIDISGDGSFQMNSQELATVVQNDIPVIVAILNNGFLGMVRQWQEL
                     FFNRRYSATCLKNSVDFVKLAEAYGALGLRAAKKSEVKDVIEEAVRSERPTVIDFVVE
                     REENVAPMVPAGAAINEILDLE"
BASE COUNT          404 a          352 c          262 g          490 t
ORIGIN      
        1 ctatcgttaa attgttatta aaataattca tatctttagg tagtgaatta ttaattccta
       61 tatctatcct gctttctgtt ttctatcctt ttatataata ataattaaaa tgtattcaaa
      121 ggtatatatg aatttccaaa catatttatg ctcagtataa tagttatcat tatgtttaaa
      181 tgaatcaatg ctggaaatcc tcagagagaa ttaaactgtt aaaagcaggc ggcaaagagg
      241 tataaaagac aggaaaaatt tgggattaat tcaaatccct accgtttctc actctggaca
      301 actttcgcgc cgcggttcat ggcgatcttt cccgtcctta ccatctctct tataccgaac
      361 tgtcgaagca attgctcaat agcattgatc ttactctcat caccggtcac ttcaattaca
      421 agtgatttta ttccaacgtc cacgattttg gccctgaaga tatctactat ctgcataacc
      481 tcggcgcgag ttgttgaatc cactccaact ttaatgagtg caagttccct gtttaccgaa
      541 tcttgtgcag ggatatcact cactcttatt acatctatga gcttattgag ttgtttttcc
      601 acctgctcaa ggatattgtc gtctcctgtc actacgatag taatgcgtga agtatccagg
      661 ttttcagtaa caccaactgc caggctttca atattgaaac ctcttcttga gaataatcct
      721 gcgacccttg ttaaaacccc gggcttattt tccacgagta tggcaagcgt atgtttcatc
      781 taatcactcc aggtccagta tctcattgat ggcagctcca gccggaacca taggcgccac
      841 attttcttcc ctctcgacca caaaatcaat aactgtgggg cgctctgatc tcacagcttc
      901 ttcgattaca tccttcactt cgcttttctt ggcagcgcgt aacccaagag caccataagc
      961 ctctgcaagc ttcacaaaat caacgctgtt cttaaggcat gttgccgaat aacgcctgtt
     1021 gaagaacaat tcctgccact gcctcaccat tccaaggaat ccgttgttca atattgcaac
     1081 tatcacggga atgtcattct ggacaactgt ggcaagctcc tgtgaattca tctgaaaaga
     1141 accgtcacct gagatatcaa taacagtaca ttcgggtttt ccaacctttg cgcctatgga
     1201 ggccgggaaa ccataaccca tggtgccaag tcctccgctt gatatgaatg tcctgggatt
     1261 cttgtagtta aagaactggg cggcccacat ctggttctgt cccacttcgg taactattat
     1321 ggcatcaggg cagatttcgc ttatttgttc aactacatat tgggggcgaa gtaaattatc
     1381 ttttttatat gtgagaggat attctttttt ccatgcattt atcttcttga tccatgcatc
     1441 tgtctttgcc tgttcaattt tcacatattt gataaggctt ttcaaaacat tttttgcatc
     1501 tccgacta
//
LOCUS       DATGBN010000253         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_253342, whole genome shotgun sequence.
ACCESSION   DATGBN010000253 DATGBN010000000
VERSION     DATGBN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253342"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..831
                     /locus_tag="VKL21_06140"
     CDS             274..831
                     /locus_tag="VKL21_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45383.1"
                     /translation="MKYKCGAVTAAILVFLVALQAAAAAMPEMQGKLNSHGSQEGLKI
                     QADDIGIASASGLNHAFIWDKTTGMKDLGTLGGSYSYPYAINDMGQVVGYSFNASGEI
                     HAFIWNRMTGMKDLGTLGGSQSLVYAINDMGQVVGQSKDASGFDHAFIWNKKTGMKDL
                     GTLGGSFSAASAINDMGQVAGSSTN"
     gene            1237..>1653
                     /locus_tag="VKL21_06145"
     CDS             1237..>1653
                     /locus_tag="VKL21_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45384.1"
                     /translation="MGDEERINQEGPLSREGWIMFLSGQITNLMNEGDKKSSVFFPII
                     SLFVVAGAFLTNSAFSTASANIQLVDKVKIIQILEIAINLLSIFFIIFIIAIVYENIK
                     FYTITKNNAERLKDIRNDIINERLTDTNEIRERWRNV"
BASE COUNT          551 a          305 c          350 g          447 t
ORIGIN      
        1 acgtttttcg agcgtggtga tcgaaaagga atttgctcaa gctaccagac gtgcaggaaa
       61 gctcagtcag gcgccttata tagtgcaggt gagccttaaa gggcacagtt ccattttcca
      121 gtgattttaa gacaccgatt aacattgatt tgcacgaatt taaaatagca tcggtgtaaa
      181 tccataaaaa atgcatatgt gatgcttgtc aaaatcactg gattttggaa cagtgtcgcc
      241 ttaaagaaga aaagtataca aaaggaggaa ataatgaaat acaaatgtgg ggctgttaca
      301 gcagccatac tggtatttct ggtcgcgctg caggcagcag cggcagcgat gccagaaatg
      361 caaggaaaat tgaacagtca tggttctcag gagggcttga aaattcaagc tgacgatatc
      421 ggaattgcgt ccgcctcagg cttaaatcac gccttcatct gggataagac gacaggaatg
      481 aaagacctgg ggacgctcgg cggaagctac agctatccat atgccatcaa cgacatggga
      541 caggttgtag gctacagctt taacgcttcg ggcgaaattc acgccttcat ctggaatagg
      601 atgaccggaa tgaaagacct ggggacgctc ggcggaagcc aaagccttgt atatgccatc
      661 aacgacatgg gacaggttgt aggccaaagc aaggacgctt caggctttga tcacgccttc
      721 atctggaata agaagacagg aatgaaagac ctggggacgc tcggcggaag cttcagcgct
      781 gcatctgcca tcaacgacat gggacaggtt gctggctcta gcacaaactg acaacagcat
      841 ctcggaagag agggttaatt ccttctcttt ttttataacc caactttgat aacaggatgt
      901 gtactataac aagcactttt ttagcctatc atcgacagca ttcatagtat aaaccctcaa
      961 tattattatc acataaataa tatttaaact caccactcac cgcagagatg ggagattctt
     1021 ggctccataa aagaaaaaat catcaatcct gctgaagaaa aagctcatga tttatcacct
     1081 tctatttctt atcgataaaa tctgatttga atattttatc cctgcataat tgccaatacg
     1141 aatggaaacg gccaccattt tataggagag tataaataca ggtcatctca ttaaataatt
     1201 tatatttaat aataattaat taaaaaaggc aaacctatgg gcgacgagga aagaataaat
     1261 caagaaggtc cactttcacg tgaaggttgg ataatgtttt tgagtggtca aattactaat
     1321 ttaatgaatg agggagacaa aaagtccagt gttttctttc caataatttc actgtttgta
     1381 gtagcgggag catttcttac aaattcggca ttttcaacag cttcagcaaa tattcaacta
     1441 gttgataaag ttaagatcat tcagatttta gaaattgcta ttaatcttct ctcgatattt
     1501 tttattattt tcatcatagc tattgtttac gaaaatataa agttctacac gataaccaaa
     1561 aataatgcag agaggttaaa agatattaga aatgatatta ttaatgaaag actaacggat
     1621 actaatgaaa ttcgtgaaag atggaggaat gtg
//
LOCUS       DATGBN010000254         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_331221, whole genome shotgun sequence.
ACCESSION   DATGBN010000254 DATGBN010000000
VERSION     DATGBN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331221"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VKL21_06150"
     CDS             complement(<1..270)
                     /locus_tag="VKL21_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45385.1"
                     /translation="MIKEILVKTALSPSKLPGLDYALNPYRGCEHACIYCFAPSVIHW
                     DKGKWGELVEAKVNLPRILSKELRVKKKGVVGLSTVTDPYHPAEKK"
     gene            complement(284..1537)
                     /locus_tag="VKL21_06155"
     CDS             complement(284..1537)
                     /locus_tag="VKL21_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nre family DNA repair protein"
                     /protein_id="HMB45386.1"
                     /translation="MESPDSLFGGAGDGLCILCRGSKLLCGKQRCPVLVKFYSNVKVK
                     PLTDSLNIDGSSPPGVFVGRIGYPYVSIGPLIPPIHGDTTLLDTPEMWIGKSIDDIVD
                     FRSQLVRGKHLVHIRDLDSSRIVEATREMALCASPIDVEAEFLKKPHARLVLDDEVQP
                     FGPTAPLKKIDVGNTKFHQGMEKAYNDTDLKAKDAVLELYKKETIISSIQKAFSVGAF
                     GEGKARRFVPTRWSITAVDSMIGANLMDKMKDFSLINEYLVFESWQLDNRFVVIMMPR
                     AWSYELIEAWYPNTVWNPFGKDVAIMSSSEGYEGRTTYAEIGGCYYAARLAVNEYLVK
                     ERKQARVVILREAHPGYIMPVGVWNVRENVRAALRAPPGKFSSLDEAFRYISTRLVIP
                     KARWIKESTILKNTLYQRGLEDFFT"
     gene            1685..2167
                     /locus_tag="VKL21_06160"
     CDS             1685..2167
                     /locus_tag="VKL21_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMB45387.1"
                     /translation="MFIEIDGWMAKLRFSACHFIPNHPKCGCLHGHTYAVSVRIEGDQ
                     QGEFIIDFETVKGIVNRICDRLDHRVLIAEKDPRLLITKNDSVSIEIIESKKKYVLPL
                     EDIMFLPTKSVSAEDLCKYFTLNLAKALELCKADNITKIHVRVDEGIGQGAGCEHEMK
                     "
     gene            complement(2180..2626)
                     /locus_tag="VKL21_06165"
     CDS             complement(2180..2626)
                     /locus_tag="VKL21_06165"
                     /inference="COORDINATES: protein motif:HMM:NF036315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5371 family protein"
                     /protein_id="HMB45388.1"
                     /translation="MKYVYVQSVLPQEDVITLKEKSGKSSIKDAIARAVRYYLKSDLD
                     NNNDQENAEALISLGMNKNAAMALIYLQNVNSVTSVDLERGAMLRQPEVSVVMKLLKE
                     RDWINEREEKKAGKGRPNKIYSLKVRFSDIITQLEKQQKKMLMKQG"
     gene            2930..>3280
                     /locus_tag="VKL21_06170"
     CDS             2930..>3280
                     /locus_tag="VKL21_06170"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMB45389.1"
                     /translation="MARRIEVGFKNGMKDALGEGIKKRIIEDLHINVETVRTIDVYTI
                     DASISSEQVIALGEKLFADPVIQVFSDKPLAQDFSWLIEVGFKPGVTDNVGGTARKAS
                     EEILGTPVQGVYFSR"
BASE COUNT          957 a          726 c          664 g          933 t
ORIGIN      
        1 tttcttttct gcgggatgat atgggtctgt caccgttgag agccctacaa cgcctttttt
       61 cttaaccctc agttcctttg aaagaattcg tggcagattc acttttgcct ctacaagctc
      121 accccatttc cccttatccc agtgaattac cgatggggca aaacaataga tacaggcatg
      181 ttcacacccc ctgtagggat tgagggcata gtccagtccc ggaagttttg agggcgaaag
      241 agcggttttt actaatattt cttttatcat tcactgtcct gtcttatgtg aagaaatcct
      301 caagtcctct ctggtataat gtatttttca agatcgtact ctccttgatc caccgcgctt
      361 tcgggataac aagccttgta gaaatatatc gaaatgcctc atcaagggaa gagaacttcc
      421 cggggggtgc cctgagggct gcccttacat tctcccttac attccacacg cctaccggca
      481 tgatgtaccc gggatgcgct tccctgagta tcacaacccg cgcctgtttc ctctccttca
      541 ccagatattc attcaccgca agccgcgccg cgtaataaca ccccccaatt tcagcatacg
      601 tggtccttcc ttcataaccc tcggaggaag acattatcgc cacatctttc ccgaacggat
      661 tccatactgt atttggatac catgcctcta taagctcata actccaggcg cggggcatca
      721 ttattaccac gaacctgtta tcaagctgcc atgactcaaa aacaagatat tcgtttataa
      781 gggaaaaatc tttcatctta tccataagat ttgcgccgat catgctgtca acagcggtta
      841 tgctccacct tgtgggaaca aaacggcgcg cttttccttc cccgaacgcc ccaacggaaa
      901 atgctttctg gatgcttgat ataattgttt cttttttata aagctcaagt acagcatctt
      961 ttgccttgag gtccgtatca ttatatgctt tctccatccc ctggtggaat ttagtgttcc
     1021 cgacatctat ctttttgaga ggggctgtgg ggccaaaagg ctggacttca tcatcaagga
     1081 caagccttgc atgcggtttt tttaagaatt cagcctccac atcgataggg gatgcgcaaa
     1141 gcgccatctc ccgtgtagct tctactatgc ggctgctatc aaggtctctt atatgaacaa
     1201 gatgttttcc ccgcacaagc tgtgagcgaa aatccacgat atcatcgata ctctttccaa
     1261 tccacatttc cggagtatca agaagggttg tatctccgtg tattggcgga atgagaggcc
     1321 ctatggaaac atatgggtag ccgatccttc ccacaaaaac accaggcgga gacgaaccat
     1381 caatattaag gctgtctgtg aggggtttta cctttacatt tgaatagaat ttgaccagaa
     1441 ccggacatct ctgtttgccg cataataatt ttgaaccgcg acacaggatg caaagaccat
     1501 cacctgcccc gccaaatagc gaatccggac tttccatatc ataactgatg ataacaatgc
     1561 atataaagtt agagcaagca tgatgataat gaacagctgc ctgtgtggca cgcataagaa
     1621 agtataaaca tgaaagtaat cacctacaaa aaggttatac taacaagagt aattaagaaa
     1681 acacatgttc attgaaatag atggatggat ggcaaaacta cgattttctg cgtgccattt
     1741 catcccgaat catcctaaat gcggatgcct tcacggtcat acatatgcag tcagtgtacg
     1801 gatcgaggga gaccagcagg gtgaattcat tattgatttc gagacggtaa aagggatagt
     1861 aaacaggatc tgcgacaggc tggaccacag ggtcctgata gccgaaaaag acccacgcct
     1921 tctcataact aaaaatgaca gcgtgtcaat agagatcatt gaaagcaaaa agaaatatgt
     1981 cctgccgtta gaagatatta tgttcctgcc cacaaaatca gtaagcgcag aagacctgtg
     2041 taaatatttc acattaaatt tggcaaaggc cctggagtta tgcaaagccg ataacatcac
     2101 gaaaattcac gtccgtgtgg atgaaggaat aggacagggc gcaggctgcg agcatgagat
     2161 gaaatgaatc actcagcatt tatccttgct tcatcaacat cttcttttgc tgtttttcaa
     2221 gctgggtgat aatatcactg aacctgactt taagcgaata tatcttattt ggtcttcctt
     2281 tccctgcctt cttctcttcc ctttcattta tccagtcacg ttcctttaaa agcttcatga
     2341 caacactaac ttcaggttga cgtagcatgg cgcctctttc aaggtcaact gaggtaacag
     2401 aattcacatt ttgaagataa atcaaagcca ttgcagcatt cttgttcatt cccagggaaa
     2461 taagcgcttc tgcattttcc tggtcgttgt tgttatccag atcgcttttt aaataatagc
     2521 gcacagctct ggcaatggca tctttgattg aagactttcc ggacttttct ttcaaagtga
     2581 ttacgtcttc ttgagggaga actgattgaa catacacata tttcatagtt atatcctggc
     2641 ataaagataa tatatataat attatttatg ctgatataac atcgttcata tatcttataa
     2701 atatatctct cttaataaat aataataatt attctcattg ttagcatgta atataattaa
     2761 taaagtagag tccaaatatt tgtctacccc ttaagaattt aatatctatc ccaagaaaaa
     2821 cagttgtcga gtctgcattt taggggatag acaaataata aatagaaaca aaacaaactt
     2881 gtcgagtcgg catgaaaatg atatttgtgt ttttgaaaat tggagaataa tggccagaag
     2941 aattgaagta ggattcaaaa atggaatgaa ggatgcactg ggcgaaggga tcaagaagag
     3001 aataatagaa gaccttcata taaatgtcga aactgtcagg actattgatg tttacacgat
     3061 cgatgccagt atttccagcg aacaggtcat tgcccttggc gaaaaactgt ttgctgaccc
     3121 tgtgatacag gttttttccg ataaacccct tgcgcaggat ttttcatggt tgatagaagt
     3181 gggcttcaag cccggggtta ccgataatgt gggcgggaca gcaagaaagg catcagaaga
     3241 aatattgggg acccctgtac agggagtcta tttttcaaga
//
LOCUS       DATGBN010000255         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_98215, whole genome shotgun sequence.
ACCESSION   DATGBN010000255 DATGBN010000000
VERSION     DATGBN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98215"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VKL21_06175"
     CDS             complement(<1..478)
                     /locus_tag="VKL21_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45390.1"
                     /translation="MRWTRSRRAVFASLVMPAAWLIFVGLTLPIRFTNNYLDFITPGI
                     LVMTMLFASLQGGTLLIFDKILGFLNKFLALPVPRESILFGKILFIAFRGLLQSTVIL
                     GMAILLGVKINYFATIPLTYFVLFIFGILFSAIAITIALLVDDYDSYASINSMASMP"
     gene            complement(511..1464)
                     /locus_tag="VKL21_06180"
     CDS             complement(511..1464)
                     /locus_tag="VKL21_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB45391.1"
                     /translation="MSVKTYELTKYFGKLCAVDHINLDVGNEIFGLLGPNGAGKTTAV
                     LILTTLLKPSTGEAYVCGYDVVKEAEKVRNVISYVPQDMAVDIKLTGKENVLLFAKLY
                     GVANRKKRVDEILELMGLSERADDRVSGYSGGMRRRLELAQALVHEPQVLFLDEPTLG
                     LDVSARRKMWEHISSLKKKGVTIFITTHYMDEADKFCDRVAIIDRGKIVAMDSPAKLK
                     SGIGKDVITANVSGEFKGLNIEGIRFLGREVDIIKFMAENGSEALPLLSEALSRQGLK
                     VHSLSVREPSLDDVFLQKVGTEEEHGSFDDHKFRNLLRRRK"
     gene            complement(1535..2902)
                     /gene="cobJ"
                     /locus_tag="VKL21_06185"
     CDS             complement(1535..2902)
                     /gene="cobJ"
                     /locus_tag="VKL21_06185"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: protein motif:HMM:TIGR01466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HMB45392.1"
                     /translation="MLIGIGLGPGNPDLLTLAAVKALKESKKVYVPGKMAAELVAPYS
                     EAEILDFPMIKDEAELSKLWENNARIVANEARECIVSFAVIGDPNFFSTFSHLRRTIE
                     ENHPDINITTIPGVSSITAQAARTNISIEGSFIVSDGSPIRTEIILKTKNPEKTRKEL
                     TKEGFDEFIYAERLFMENEKVTCDIPQKGDYFSMLVAKRSKSMSSSKLRGKLYIVGIG
                     PGATEHLTRRAENALLESEYVIGNGTYLDQVSEIIKNSKIIRSGMGSEIERAKKAVEL
                     GKDHVVSIISGGDANVYGMAGLVLEVAEKAGSIDIEVIPGITALSAAASLLGAPLVSD
                     FAVISLSDLLTPIKIIENRLNSAAEADFTIAIYNPKSRNRAQNFGTAIEIIQKYRSKD
                     IPVGIVKNATREGETVIATTLGRIMEYNDLIDMSTIVLIGNSESRLWDNRIITPRGYQ
                     RKYDY"
     gene            complement(2902..3462)
                     /locus_tag="VKL21_06190"
     CDS             complement(2902..3462)
                     /locus_tag="VKL21_06190"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HMB45393.1"
                     /translation="MKWVLLIIKSTNVSSRDRMFIWRNIKNTGAVSLSHSVYILHDTE
                     DNRATAIDIARIAHERKGEVLQFFADTLNEEQEQKLNKLIAEEIATEIKEFSKECEDF
                     IDDITKRISNKKIKIFELEELSDDIHKLDRWRIKLIQKHGLDAPKIEILNKKIGKCKE
                     KLNEFEEKILQKNEILKGSERVRGDK"
     gene            3574..3951
                     /locus_tag="VKL21_06195"
     CDS             3574..3951
                     /locus_tag="VKL21_06195"
                     /inference="COORDINATES: protein motif:HMM:NF020334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HMB45394.1"
                     /translation="MVVISISLSGTELKEFDNITNKMGFSSRSDAVREALHRFVAQNK
                     WIEHIDSGTPFIATIVYDDKREQPVHNIIHDFKNIVKSATHTHFGDRCAEWVILQGDN
                     DSIKQFVERISAIKDVMVCRCSV"
     gene            4018..>4822
                     /locus_tag="VKL21_06200"
     CDS             4018..>4822
                     /locus_tag="VKL21_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HMB45395.1"
                     /translation="MVELKDFELKIKGMDCPSCAMNVENAVRKLNGIGEINVNFITGK
                     AAIKYDPSLTNTSEIREAIEKAGYTALEDRKDDHEGSCSSCSTDIFEEKLPLWKQWKI
                     RIIALSSVLLVLGLSIEYVTGTTALSHILFLLAGVISGYSIAKKGISSLLKKRLDMNF
                     LMTIAAVGAFSIGYGEEGASVLYLFFIAEFLEDYAGERARKSIGALVKLAPETAIVIR
                     DGKEVNMHVHDVNINDITVVRPGEKIPLDGIVINGESSVNQAAITGESMP"
BASE COUNT         1351 a         1085 c          856 g         1530 t
ORIGIN      
        1 gtggcatact tgccatgctg tttatcgatg catagctatc gtaatcatct acaagcaaag
       61 ctatcgttat ggcgattgcg gagaacaata ttccgaatat gaacaaaaca aagtaggtaa
      121 gaggaatagt cgcgaaataa ttaatcttca ctcccagcag tatcgccatt ccaagaatta
      181 cagttgactg taacaggcct ctaaaagcaa taaaaaggat tttgccaaaa agtatgctct
      241 ctcttgggac tggcaaagct aaaaatttat ttaaaaaacc aagtatttta tcaaaaataa
      301 gcagcgtccc accctgcagt gaggcaaaaa gcattgtcat gacaagaatt cccggtgtaa
      361 taaaatcaag ataattgttt gtgaatctga ttggtaaagt aagacccacg aatataagcc
      421 aggctgccgg catcacaagg gatgcaaaca cagctctcct ggacctggtc catcgcatca
      481 aatctctttc gaaataatac tgaactcccc tcattttctt ctccgcagca gatttctgaa
      541 tttatgatca tcaaaacttc catgctcttc ctccgtacca accttttgca ggaaaacatc
      601 atcaagtgat ggttctctta ctgataaaga atgaactttt aatccctgcc tcgataatgc
      661 ttcagataac agaggcaaag cctcgcttcc gttctcagcc atgaatttaa taatatcaac
      721 ctctctccca agaaatctta ttccttcaat attcaatccc ttaaattcac ctgacacgtt
      781 tgcggttatg acatcttttc ctatgccact ttttaatttt gcaggcgaat ccattgccac
      841 gattttgccg cgatctatta ttgctactct atcgcaaaat ttgtcggcct cgtccatgta
      901 gtgtgttgtt ataaaaatgg tcacaccttt tttcttcagg gaagatatgt gttcccacat
      961 ttttcttctt gctgatacat ccagaccaag agtgggttcg tcaagaaaca acacttgtgg
     1021 ctcatgtacc agagcctgag caagctcaag cctccttcgc attcctcctg aataaccact
     1081 tactctatcg tcagccctct cgctcaagcc catcaattca agaatttcat ctactctctt
     1141 tttcctattt gctacaccgt ataactttgc aaacagcaat acgttttctt ttcctgttaa
     1201 tttaatatcc accgccatat cttgaggcac atagcttatg acgttacgaa ctttctcagc
     1261 ctctttcacg acatcataac cgcaaacata agcctctcca gtgcttggtt ttaacagagt
     1321 ggttagaata agcacagctg tggttttgcc tgcaccgttc ggacctaata atccaaaaat
     1381 ttcatttcct acatccaggt ttatgtgatc aactgcacac agcttaccaa aatacttggt
     1441 aagctcatat gttttaacgc tcatagaatc gtcctcccgc caatatcaca tattcgatgc
     1501 aatacgtttg gtgaagagtc gagaagatat tttactaata atcatatttt ctctggtatc
     1561 ctcgcggtgt aattatcctg ttatcccaca gtctcgactc gctgttgcca attagcacaa
     1621 ttgtgctcat atcaatcaaa tcattgtact ccattattct accaagagtt gtagcaataa
     1681 ccgtctcccc ttcgcgtgtt gcatttttca caattcccac tggaatatcc ttgctccggt
     1741 atttttgtat aatctctatc gctgtcccaa aattctgcgc gcggttcctg cttttcggat
     1801 tatagatagc aatcgtaaaa tcagcttctg cagcgctgtt gagccgattc tctattatct
     1861 ttatgggtgt cagtaagtca ctcaggctta taaccgcaaa gtcggatact aaaggcgcac
     1921 caagaagaga agcagccgcg ctcagagcag taatccctgg aataacctcg atatctatac
     1981 tgcccgcctt ctctgccacc tcaagtacca gccctgccat tccgtacaca tttgcatccc
     2041 ctccgctgat gattgaaaca acatggtctt ttccgagttc aaccgctttc ttagcacgct
     2101 ctatctcgct tcccattccg cttcttatta ttttcgaatt ttttataatt tcgctgacct
     2161 ggtcaagata cgtgccatta ccaatgacat attcagattc aagtaatgca ttttcagctc
     2221 tccgggtaag atgttcagta gcgcctggtc ctatacctac aatgtaaagt ttaccccgca
     2281 gtttcgatga tgacattgat ttgctgcgct ttgctaccag catgctgaaa taatcgccct
     2341 tttgcggtat gtcacaggtt accttttcat tttccatgaa cagccgctct gcatagataa
     2401 attcgtcaaa accttctttt gtcagttctt ttcttgtttt ttctgggttt ttcgttttca
     2461 ggataatctc tgttctgata ggtgagccgt cactcacaat gaaagagcct tcgatgctta
     2521 tatttgtcct tgctgcctgt gcagttatcg aactcacccc gggaatcgtg gtgatattga
     2581 tatcagggtg attttcttct attgtcctgc gaaggtgtga aaaagtggag aagaaattcg
     2641 gatctccaat tactgcaaaa gagacaatac attcccttgc ttcatttgcc actatccttg
     2701 cattgttttc ccataatttt gatagctctg cttcatcctt aatcatagga aaatccagaa
     2761 tctctgcttc tgaataaggc gcaacaagtt cagcagccat ttttcccggc acatatactt
     2821 tcttgctctc tttcaatgct tttacagcag ccagtgtgag aaggtcagga tttccgggac
     2881 caagacctat tccgattaac atcacttatc gcctctcaca cgctcactcc cttttaaaat
     2941 ttcgttcttt tggagaattt tctcttcaaa ttcattcaat ttttctttac atttcccgat
     3001 ttttttattc aaaatctcta ttttaggtgc gtcaagacca tgtttctgaa ttagctttat
     3061 tctccacctg tcaagtttat gaatgtcgtc actcaattcc tctaattcaa aaatcttgat
     3121 tttcttattt gaaattctct tagttatatc gtcaataaaa tcttcgcact ctttggagaa
     3181 ttctttaatt tctgtagcaa tttcttctgc aatcagttta tttaacttct gctcctgctc
     3241 ttcattaaga gtatcggcaa aaaattgtaa aacttcacct tttctttcat gtgctattct
     3301 ggcaatatct attgcagtag cccggttatc ttcagtatca tgcaatatat agactgaatg
     3361 agataacgaa actgcgccgg tgttttttat atttcgccag atgaacatcc tatctcttga
     3421 tgaaacgttt gtcgatttaa taattagtaa tacccatttc atatatcctt gaacaagtat
     3481 atgatattta aatgttatgt ttataacaaa tttaaatgca actttttaca catttacaaa
     3541 agtataagtt gatcaggcat catatagtat ttcatggtag ttataagcat atcactctca
     3601 ggcacagaac taaaggaatt cgataatatc acgaataaaa tgggcttttc cagccgttct
     3661 gatgcagtcc gtgaggccct tcatagattc gttgcccaga ataaatggat agagcatatt
     3721 gatagtggta ctccattcat cgcgactatt gtttatgacg ataaaagaga acagccagta
     3781 cacaacatca tccatgattt taaaaatatt gtaaaatctg caacccacac tcattttggc
     3841 gacaggtgtg ctgaatgggt aatccttcag ggagataatg acagtatcaa acagtttgta
     3901 gaacgcatat ctgcaataaa ggatgttatg gtctgccgct gttccgtcta atttttatta
     3961 actttcagcc agtttcttag cacaatctac ttttaaaagc gtgtaataca ggtctgcatg
     4021 gttgagctaa aggattttga attaaaaatc aaaggcatgg actgcccttc ctgcgccatg
     4081 aacgtggaaa atgcggtcag gaaactgaac ggtatcggtg agataaacgt gaatttcatt
     4141 actggtaaag cagcgataaa gtatgaccca tccctcacta acacatcaga aatcagagag
     4201 gcgattgaaa aagccggata taccgctctt gaagatagga aggatgatca cgaaggaagc
     4261 tgttcctcat gttcaaccga tatttttgaa gaaaaacttc ctctctggaa acaatggaaa
     4321 atacgtatta tagccctttc atccgtgtta ctggttcttg ggctttccat agaatacgta
     4381 acgggaacaa cagctttatc tcacattctc ttcctgctcg caggagtaat ttcaggatac
     4441 agcatagcta aaaagggcat atcttctctc ctgaaaaaac gccttgatat gaatttcctg
     4501 atgacaattg ctgctgttgg tgctttctcc atcggctatg gggaagaggg agcatctgtg
     4561 ctttatctgt tcttcattgc agagttttta gaagattatg caggcgaaag ggcgaggaaa
     4621 tccatcgggg cgcttgttaa gcttgcgcct gagactgcca ttgtaataag ggacggaaaa
     4681 gaggtcaaca tgcatgtcca tgatgtgaat atcaatgata ttacagttgt tagaccgggt
     4741 gagaagatac cccttgatgg tattgtgatt aacggagaat caagcgttaa tcaggccgct
     4801 atcaccggag aatcaatgcc tg
//
LOCUS       DATGBN010000256         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_370105, whole genome shotgun sequence.
ACCESSION   DATGBN010000256 DATGBN010000000
VERSION     DATGBN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370105"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VKL21_06205"
     CDS             complement(<1..787)
                     /locus_tag="VKL21_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HMB45396.1"
                     /translation="MPFKILKKQELVPTIHLMDVSAPRIARKAQPGQFVIIRINETGE
                     RIPLTIADFDRKKGTITMIFQAMGKTTTQLSTLGTGDELMDFIGPLGNPAHIENEGTV
                     ILVGGGVGVAPVFPQARAFKEAGNKVLSIIGARSANLLLWEDRMRDVSDELYITTDDG
                     TKGHHGFVTDIVKKILESPTKVDRVIAIGPPVMMRAVAGVTKPFNVKTIVSLNSIMVD
                     GTGMCGACRVLVGNETKFACVDGPEFDAHLVDFTLLMNRLAIYQ"
     gene            complement(893..1852)
                     /gene="thiL"
                     /locus_tag="VKL21_06210"
     CDS             complement(893..1852)
                     /gene="thiL"
                     /locus_tag="VKL21_06210"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMB45397.1"
                     /translation="MKISDLNERALIEHIEGFLARPGKKTIVGAGEDDCAVLDIGGDD
                     YLLSTTDMLHRKTDFPPQMSGWQIGWMSAAVNLSDLASKGAKPLGILMAVGIPPDTEL
                     DFLDEIIKGMDDCVHSCDTQVIGGDMDSHEELTITGTALGLVNKDLLIRRSGATPGDI
                     VCVTGNLGTAGAALISLQKKIAVNKKILKALFEPAPRINEGMALAKTRAITSMMDISD
                     GLALSLHDIAKASSVGFKIYEDKLPVLWDVKKLLKGDELLEAVVFTGGDFELLFTVRA
                     DKINEARNACRFSVIGEVIKEGIVIERSDGIEELKCRGYEHFN"
     gene            2044..2316
                     /locus_tag="VKL21_06215"
     CDS             2044..2316
                     /locus_tag="VKL21_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45398.1"
                     /translation="MFETSEKRVKLLKKGFTQKQIEELYIKGNNFKIVNLPLLIELVE
                     IKDRKNKKMCVNCLTAVGYAQSFCEEMVNISCISHFSEELSKSRTM"
     gene            complement(2297..>2845)
                     /locus_tag="VKL21_06220"
     CDS             complement(2297..>2845)
                     /locus_tag="VKL21_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMB45399.1"
                     /translation="LSKEIKKINNDVEWGLPVNESLIRFANRYRTHFISRSVNLLTKA
                     NESSGDIGEVLNVAARDVAAEQELKNERKVNMFIYVVIIYISFMVFIGIIYIISTTFL
                     AEMVKAGEKMAAGGSGSVPLSLSREKLNMYNLIFFHGALIQGLSSGLIAGVMGEGNAL
                     SGLKHSVIMMTIGYLLFTLFVI"
BASE COUNT          810 a          647 c          527 g          861 t
ORIGIN      
        1 tctggtaaat agcaagccta ttcatcagca gggtaaaatc cacaagatgc gcgtcaaatt
       61 caggaccatc cacgcaagcg aactttgttt cattgccaac aagcaccctg caggcgccgc
      121 acataccggt tccgtcaacc attattgaat taaggcttac tatggtcttt acattaaatg
      181 gctttgtaac gcctgcgacc gcccgcatca ttacaggagg accaatagcg atcaccctgt
      241 ccacttttgt tgggctttcc aggatttttt tgacaatatc ggtcacaaaa ccatggtggc
      301 ctttcgtgcc gtcatccgtg gtaatataca gctcatcgct gacatctctc atcctgtctt
      361 cccatagcag gaggtttgca ctgcgcgctc caatgatgga aagcacttta tttccggctt
      421 ccttgaaagc tctggcctgg gggaaaacag gcgccacccc gacacctcct cccacaagga
      481 tcacagtgcc ttcattttca atgtgggccg gattcccaag cggtcctatg aaatccatga
      541 gttcatcgcc ggttccaaga gtggataatt gtgttgttgt ctttcccatt gcctggaaga
      601 tcatcgtaat agttcctttt ttacgatcaa aatcagcgat ggtgaggggg atcctttccc
      661 cggtttcatt tatcctgatg atgacgaact gtccgggctg tgcttttcgt gcaatacgcg
      721 gggcgctcac atccatcaaa tggatcgtag gaacaagttc ctgttttttt aagatcttaa
      781 atggcatttt ttcatctctt caaaatagct ctaagtagct tttgaatata aaaattatct
      841 gtaccccggt tacatgaact ctggaatctc ccggcttcag ccgtggagag tctcaattga
      901 agtgttcata tccccggcat ttaagttctt ctattccatc cgatctttca attacaatgc
      961 cttcttttat gacttcccca attacggaaa aacgacaggc atttctggct tcattgatct
     1021 tatctgcacg cacggtgaat aataactcaa aatccccgcc cgtaaaaaca accgcttcta
     1081 gcaattcatc gccttttaat agttttttga catcccagag gacaggaagc ttatcttcgt
     1141 atattttaaa gccaacagag cttgcttttg ctatatcgtg aagggataag gccagcccgt
     1201 cgcttatatc catcatagat gttattgcgc gtgtttttgc aagcgccata ccttcattta
     1261 ttcttggcgc gggttcaaat aaggctttta atatcttttt atttactgca attttttttt
     1321 gtaatgaaat aagcgccgcc ccggcagttc caagatttcc tgttacgcaa acgatgtctc
     1381 ctggagttgc cccgctcctc cttatgagca agtccttatt tacaagtcca agcgcagttc
     1441 ccgtaatcgt cagttcctca tgtgaatcca tatcgccacc gattacctgt gtatcgcagc
     1501 tatgtacgca gtcatccata ccttttatga tctcatccaa gaaatcaagt tctgtatcgg
     1561 gtggaatgcc aacagccatt agaattccca gaggctttgc accttttgaa gcaaggtcgc
     1621 taaggttcac agccgctgac atccacccta tctgccagcc tgacatctga ggcggaaaat
     1681 ctgtctttcg atgaagcata tccgtagttg aaagaagata atcatctcct cctatatcaa
     1741 gaacagcaca atcgtcttct ccagcgccaa cgatcgtttt ttttccgggc ctggcaagga
     1801 atccttcaat atgttcaatt aaggcccgct cgttaagatc actgattttc atttgataac
     1861 taattcaatt tatacacatt ataatttttc tataattttc aaaaaaagca taatttataa
     1921 ccaggaaaaa ttaaaaagtc tcttatatat atagttaccg aagacttata tatgatgatg
     1981 ctcatcatat aatacaggat aataatgtaa ttctaaaaaa tgaatgtgtg tagaggaata
     2041 ataatgtttg aaacgtctga aaaaagagta aaattattaa aaaaaggttt tacacaaaaa
     2101 caaatagagg aactgtatat taaaggaaat aatttcaaaa tagtaaatct gcccttactt
     2161 attgaacttg ttgaaattaa ggataggaaa aataagaaga tgtgcgtgaa ttgcttaact
     2221 gcagttgggt atgcccaatc gttctgtgag gaaatggtaa atattagctg tatatcacat
     2281 ttttctgaag agttatctaa atcacgaaca atgtgaagag cagataacca atagtcatca
     2341 ttatgaccga atgtttcaat cctgaaaggg catttccttc acccatgacg ccggctatca
     2401 aacccgatga aagaccctgt atcagggcgc catggaagaa tattagatta tacatattca
     2461 gcttctcgcg gctaagactg agaggaacag acccgctgcc tcctgctgcc atcttttctc
     2521 cggcttttac catctcggca agaaaagtgg tagaaattat gtatataatt ccaatgaaca
     2581 ccatgaagga tatgtaaata atcacaacat aaatgaacat atttactttg cgttcattct
     2641 tcaattcctg ttccgctgca acatctcttg ctgcaacatt caatacttcc cctatatctc
     2701 cgcttgattc atttgctttt gttaataaat ttacggaccg ggagataaaa tgggtcctgt
     2761 aacggttggc gaaccgtata agggattcat taacaggaag tccccattcg acatcattat
     2821 ttatcttctt aatttccttg ctcaa
//
LOCUS       DATGBN010000257         6216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_447653, whole genome shotgun sequence.
ACCESSION   DATGBN010000257 DATGBN010000000
VERSION     DATGBN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447653"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VKL21_06225"
     CDS             complement(<1..271)
                     /locus_tag="VKL21_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45400.1"
                     /translation="MGLNLFDIAVWWLVLEITGFVTLPISFYVCRNLSDHGYSVSKPL
                     GLLLVTYLSWIISIVLGYSYISILLSFVVIAAFSLFIYKKKGIAFF"
     gene            complement(307..1875)
                     /locus_tag="VKL21_06230"
     CDS             complement(307..1875)
                     /locus_tag="VKL21_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45401.1"
                     /translation="MIGIVNNSDEDGFTFISQERLPAGMFACYHEADRKILCRVKNAE
                     PLKQYPQEFLMDMELSPDDVCAFYGFDPQDFKYYSYSASVVGYFDDAMGEFINPRTNP
                     ASGVKIHRAGEDVLKDISKVKPGDSGSAFIGNVLGEKTGIALSVRDIVSQHVSVIAST
                     GAGKSYTVGVLVEELLRPYNMAPVLIFDPHGEYSSLGDISNTAEFVTTSYRPKVRTVK
                     PKDIKIRINDLLVGDFISIMDDGTMSDKMKMLFRTAYHGLKNDKKKQFTRNELKLRIE
                     SLRDSNNESTIDGINWRFGKLNAAIFDDFQTIPLADYFKVGQLTIMDVSGIEEGFQQL
                     IASVMLRRLFDAREGTENNRYYEMHIDKFLPYPVFVIIEEAHRFAPQSGEAKSKSILK
                     TILSEGRKFGIGVCLVSQRPSKLDADSLSQCMTQITMRIINPTDQQQIAQSIESASRE
                     LISELPSLAKGQAIISGVAINTPALVKIRKRLTSDTKGRSKDAPALWAAQRKYEDKHR
                     APEVKADEDMDVGV"
     gene            complement(1872..2387)
                     /locus_tag="VKL21_06235"
     CDS             complement(1872..2387)
                     /locus_tag="VKL21_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45402.1"
                     /translation="MVIGDIIRVEDASTYVAKIPPDAKKERIGSFIKIQKDDGLIVGV
                     IKNITNSIREDLIPYISSELQPKYAPFNEDFRNSYYFIHGLGTIRNGIVEYNIDSPPD
                     VRDNVEILNPEELKFFHTIDGKQSIAYFHSNSEALPQNVLIAMIDQVDEKCPECKAMS
                     KLVRKYIRRDL"
     gene            complement(2392..3540)
                     /locus_tag="VKL21_06240"
     CDS             complement(2392..3540)
                     /locus_tag="VKL21_06240"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HMB45403.1"
                     /translation="MLDLKNISIQFDEKLSTIKSYDAERSELTSEYRSELEKLKIINP
                     ELNKNLPSYSGAKFLEPGRLIHPFQFNWKDRKEAMEWVDSVLCGVPVGAVDGSQIYSD
                     KNFEIPLAVIQVSRIFNRHSQNMDYKQETDASIITPDEFEAASVYSFGREYVDARRFY
                     MECEGVVHLMNEYEGIFVLLDGALILSHINMLNKNTREIYVKAIMKLLGASQKTRNPV
                     IGFIDTTMPRDITLMMHFLFGLRKSKLPDTQLFSNILWGERTAAFLCDRDDRRGDEAR
                     SVLDNYGIFREELAFFYMRASNGLPVRVEFPSWVHKDGMVDKIADIIRAECIIRSNYP
                     DILLRAHDAAVIRTNEHELFYGMLENFCKVHGIKIHRSAKDFHKKLNR"
     gene            complement(3528..>6216)
                     /locus_tag="VKL21_06245"
     CDS             complement(3528..>6216)
                     /locus_tag="VKL21_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45404.1"
                     /translation="DVIDELASSEKEFIKETEKLKNLNIFRTERDKLKEKYTTIELEI
                     SRLNEKYERSKTILLENSEIEERKSLLIPKIEAARRLEESIQLLQKELKDPMTDLISR
                     LSNLREKAQLVENMKQEIEKQNQKISTLLPLKNKQIELENRITELKEIEISLKKLSLE
                     ISALKEKEVLAGKLRNEIERLESRKKELFWVMERQKNLSDEIKKLSTLLESLTAINFQ
                     FRIAREKEERVKTIKAEITKLENEINSLIPLAEKQATLEKVQQELNWNHEAIKSSITQ
                     IKKNMKLAGTQGFCPILNSVKCSSISDFNKYFNDEIDQKSRELEDMETSLKNNKKELA
                     KLDDPQKKTQEKLVLIASRKKDLESYKGIEKELEVIQEKLDSLSSLYTETLTGSDEEL
                     KEISKNQSSRKKEFEELARSITEFQNITALAGSRNKDLEELHDIPAVLIGLQEKLAAI
                     NEKFNLDIEINSINNHLDTVVSEVKSSEKDLKYLNNPVHQIAIIENLIVSKRSDIDSR
                     KEVPQLIDGCLKQLDELNQRFHLKESFKGEKGEIELVDNLMDDKKKELKSLASPDKQF
                     DTLNENIKKNLKELKTLEKVPELLDSNNKEREALMQNLSKFEGLGENILSLENTINKL
                     EPLHNKYLQALPLASKFDEYTQETLEFENTIAATGASLKELITKHESLSKEFKESDLN
                     ETTMKLKELGKTASSMTEGVKQKNKNLQKIIKELSQMESILIKIKENQRNLEKERDFL
                     SFSNFIRDTIKSSSEFVINEFIGEISSEANNIYCEIMDDHSASLKWANDYDIKIESGG
                     EICTFKQLSGGERMSAALSVRLALLKILSNCDFVFLDEPTQNMDEIRRENLSEQITKI
                     KGFKQVFVISHDDTFNEKYAHVIKVQKNNGESRVESCST"
BASE COUNT         1592 a         1348 c         1167 g         2109 t
ORIGIN      
        1 caaaaaaagc tattcctttt tttttatata tgaagagtga aaaagcagct ataacaacaa
       61 aggaaagcag tatggaaata taagaataac caagaacaat agaaataatc cagctaagat
      121 atgtaacgag tagcaatcca agtggctttg aaacagagta accatgatcc gataaatttc
      181 tgcacacata aaacgagatt ggaagtgtta caaaacctgt gatctctaag acaagccacc
      241 agactgcaat atcaaacagg ttcaatccca taagaactta gcccctgtag ggaagtttac
      301 caaatattaa acccccacat ccatatcttc atccgccttc acttcaggcg ccctgtgctt
      361 atcttcatac ttcctctgtg ctgcccacag cgccggagca tccttgctcc tcccttttgt
      421 atcgcttgtg agtctctttc gtatttttac cagcgccgga gtatttatcg caacgcctga
      481 aatgatcgcc tggcctttcg caagagatgg cagctctgaa ataagttccc tgcttgcgga
      541 ttcaatgctc tgggctatct gctgctggtc agtcgggttg attattcgca tcgtgatctg
      601 ggtcatacat tgcgaaaggg aatccgcatc cagtttgctg gggcgctgtg acacaaggca
      661 caccccaatc ccgaatttcc tgccttctga gagaatggtc ttaaggatgc tctttgattt
      721 tgcctcgccg ctttgcggag cgaacctgtg cgcctcctcg atgatcacaa aaacaggata
      781 aggaaggaac ttatcgatat gcatttcata atacctgttg ttctcggttc cctcacgtgc
      841 atcaaagagg cgtctgagca tgactgatgc tatcaactgc tggaatcctt cttcaattcc
      901 tgagacatcc attatagtga gctgccctac tttgaaataa tcggcaagag gaatggtctg
      961 gaaatcatcg aagatcgctg catttaattt cccgaatctc caatttatac catcgatcgt
     1021 agattcatta tttgaatccc gaagagattc tatacggagt ttcaattcat ttctggtaaa
     1081 ctgttttttc ttgtcatttt tcaggccgtg ataggcagtt cgaaatagca tcttcatctt
     1141 atcagacatt gtgccatcat ccataatgct gataaaatcg ccaacaagaa ggtcatttat
     1201 tcttattttt atatcctttg gcttaactgt cctgacttta ggacggtaag aagtagttac
     1261 aaattcagcc gtgtttgaga tatcaccaag tgaagagtat tccccatggg ggtcaaaaat
     1321 aagaacgggg gccatgttat aaggtcttaa taactcttca accagaacgc cgaccgtgta
     1381 tgattttccc gcgcctgtgg atgcgatcac agacacatgc tggctcacga tatctcgtac
     1441 ggataacgcg atcccggtct tttcgccaag cacatttccg ataaatgcag agccggaatc
     1501 acccggtttt actttgctta tatctttcag gacatcctcg ccggcacgat ggattttcac
     1561 accgctggca ggatttgtcc ttggatttat gaattcaccc atagcatcat caaaataccc
     1621 cacgactgat gctgaatatg aatagtattt aaaatcctgg gggtcaaatc cgtaaaatgc
     1681 gcaaacgtca tcaggggata gctccatatc cataaggaat tcctgaggat attgtttgag
     1741 cggttctgcg ttctttaccc ggcacaggat tttcctgtct gcctcgtggt aacatgcgaa
     1801 catcccggca ggaagccgct cttgtgatat gaatgtaaat ccatcttcat ccgaattatt
     1861 gacgatgcct atcataaatc cctccggata tattttcgaa caagctttga catagctttg
     1921 cattcaggac atttctcatc tacctgatcg atcattgcaa taagaacatt ttgaggaaga
     1981 gcttcactgt tggagtgaaa ataagcgata ctttgcttcc cgtcaattgt atggaaaaac
     2041 tttaattctt cagggtttaa aatctcaaca ttgtcccgca catcaggtgg tgaatcgata
     2101 ttgtattcta caattccgtt acgaattgtt ccaagtccat ggataaaata atagctgttc
     2161 ctgaaatcct cgttaaaagg tgcatatttg ggctgtaact cagaacttat atatggaatc
     2221 aggtcttccc gaattgagtt tgtgatgttc tttattacac ctactatcaa tccgtcatct
     2281 ttttgtattt ttatgaaact gccgatgcgt tccttttttg catcgggcgg tattttcgcc
     2341 acatacgtgc tcgcatcttc aaccctgatt atgtctccta ttaccatcga atcacctgtt
     2401 taatttttta tggaaatctt ttgcacttct atgtatcttt atcccatgaa ctttgcagaa
     2461 attctcaagc atcccgtaaa aaagctcatg ttcattcgtt cggatcacag ctgcatcgtg
     2521 agcccgcagc aatatatcgg gataattgct tcgaatgatg cattctgccc tgataatatc
     2581 tgcgatctta tcaaccatgc catctttatg aacccatgat ggaaattcca cacgcacggg
     2641 aagaccattg cttgccctca tgtaaaaaaa agccaattct tccctgaata tgccatagtt
     2701 atcaagaacg gatctcgctt catcaccgcg cctgtcatcc ctgtcgcaga gaaacgctgc
     2761 tgtgcgctca ccccatagta tatttgaaaa aagctgcgta tccgggagtt tacttttccg
     2821 taatccgaaa aggaaatgca tcatcagagt aatgtccctg ggcatagttg tatcgataaa
     2881 accgatcacc ggattccttg ttttttgtga tgcaccaagt agtttcatta tcgcttttac
     2941 atatatttcc cttgtgttct tgttcagcat attgatatga gaaagaataa gcgcgccgtc
     3001 aagcaagaca aatatccctt cgtattcatt cattaagtga acaacacctt cgcattccat
     3061 gtaaaaacgg cgcgcatcga catattctct gccgaaggaa taaacgcttg ccgcctcgaa
     3121 ctcatctggc gtgattatcg atgcgtctgt ttcctgctta taatccatgt tttgcgaatg
     3181 cctgttaaaa atgcgcgata cctggataac agccagaggt atctcaaaat tcttatccga
     3241 atatatctgg ctaccatcaa ctgcccccac aggaacaccg caaaggacag aatccaccca
     3301 ttccatcgcc tctttgcggt ccttccagtt aaattggaat ggatgaatga gtcttcccgg
     3361 ttcgagaaat ttcgctccac tgtaggaggg aaggttctta tttagttccg gattgattat
     3421 tttcagtttt tcaagctctg acctgtattc agatgttaat tcagagcgct cggcgtcata
     3481 ggattttatt gtggaaagct tctcatcgaa ctggatagaa atgtttttca agtcgagcat
     3541 gattcaaccc tgctttcgcc attgttcttc tggactttta tgacgtgcgc gtatttttcg
     3601 ttgaaagtat catcatggga tatgacaaaa acctgcttga aacctttgat cttggttatc
     3661 tgctcggata ggttctcccg ccgtatttca tccatgttct gggtgggctc atccaggaag
     3721 acgaaatcgc agttagaaag gatttttaaa agcgcaagcc gcacagaaag cgcagcgctc
     3781 atgcgttctc cgccgcttaa ctgcttgaag gtgcaaattt ccccccctga ttcgatcttg
     3841 atatcataat catttgccca tttaagggat gcgctatggt catccatgat ctcgcaataa
     3901 atgttattgg cttcactgct tatttccccg atgaattcat ttatcacgaa ttccgaagag
     3961 gattttatgg tatcacggat gaaattcgag aaggataaga aatccctttc cttttcaaga
     4021 tttctttgat tttcctttat tttaattagg atggattcca tttgtgaaag ttcctttata
     4081 attttctgaa ggttcttgtt cttctgtttt actccttcag tcatactgct tgctgttttt
     4141 ccaagttcct taagtttcat ggtagtttca tttaggtcag attccttgaa ttcttttgaa
     4201 agcgattcat gtttagtgat caattctttt aaagaagctc ccgttgcagc gatggtattt
     4261 tcaaattcca gcgtttcctg tgtatattca tcgaatttgg acgcaagcgg gagggcctgc
     4321 aaatatttat tatgcagcgg ttcaagttta tttattgtgt tctcaagcga taaaatgttt
     4381 tcaccaagcc cttcgaattt cgagagattt tgcatcaatg cctctcgttc tttattgttt
     4441 gaatccagaa gctccggtac tttttcaagt gttttcagtt ccttaaggtt tttcttgatg
     4501 ttttcattca aggtgtcaaa ttgcttatcc ggggatgcaa gtgatttcag ctctttcttc
     4561 ttgtcgtcca tcaaattgtc aacaagttcg atctcccctt tttcgccctt aaacgactct
     4621 ttcaaatgga acctttgatt gagttcatca agttgtttca aacaaccatc gatcaactgc
     4681 ggcacttctt tgcgggaatc aatatctgat ctctttgaaa caatgaggtt ttcaattatg
     4741 gcaatttgat gcacggggtt attgagatat ttcaggtctt tttcactgga tttgacctct
     4801 gaaacaacag tatcaaggtg gttattgatt gaattgatct caatatcaag gttgaatttt
     4861 tcgtttattg cggctaattt ttcctgcaat ccaataagta cggcaggaat atcatgtaac
     4921 tcttcaagat ctttgtttct tgatcctgcc aatgctgtaa tattttgaaa ttctgtgata
     4981 gatcttgcca gttcttcaaa ttcttttttc ctggagctct gattttttga tatctctttg
     5041 agttcctcgt cgcttcctgt taatgtttcg gtatataatg aggataggga atccaatttt
     5101 tcttgaatta cttcaagctc tttttctatt cctttatatg attcaagatc cttctttctt
     5161 gatgcgatca gcacaagttt ttcctgtgtc tttttttgtg gatcatcgag tttagcaagt
     5221 tccttcttat tgtttttcaa acttgtttcc atatcttcta attcacggct cttctgatcg
     5281 atctcatcat tgaaatattt attaaaatca cttattgagg aacatttgac actattcaat
     5341 attggacaga atccctgcgt tcctgcaagc ttcatatttt tctttatctg cgtaattgac
     5401 gattttatag cctcgtgatt ccaattaagt tcctgctgca ctttctcaag tgtggcctgt
     5461 ttttcagcaa ggggaataag agaattgatc tcattttcaa gctttgtaat ctctgctttg
     5521 atggttttta cacgctcttc tttttcacgc gctatcctaa attgaaaatt aatagcagta
     5581 agtgattcaa gcagggttga caattttttt atttcatcag aaaggttttt ttgcctttcc
     5641 atgacccaga aaagctcttt ttttcgtgat tcaaggcgct ctatttcgtt acgtagtttt
     5701 ccggctagaa cttccttttc tttcaaagca gatatttcaa gagagagctt ctttaatgat
     5761 atttctattt ccttcaattc tgtaattcta ttctcaagct cgatttgttt gttttttaat
     5821 ggcaaaagtg tcgatatctt ctggttttgc ttctcgatct cttgtttcat attctccaca
     5881 agctgcgctt tttcgcgaag gtttgataat cgcgaaatga ggtcagtcat cggatctttc
     5941 agttcttttt gaaggagttg gatcgattct tcaagcctgc gtgcagcttc tatttttggg
     6001 ataaggaggc tctttcgttc ctctatttcc gaattttcca ataaaatcgt ctttgagcgt
     6061 tcatattttt cattaagacg gcttatttca agctcgatcg ttgtgtattt ttcttttaat
     6121 ttatctcgtt ccgtacggaa gatattgagg ttttttaact tctccgtctc tttaatgaac
     6181 tccttttcag aagacgcaag ttcatcaatg acatcc
//
LOCUS       DATGBN010000258         6590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_137542, whole genome shotgun sequence.
ACCESSION   DATGBN010000258 DATGBN010000000
VERSION     DATGBN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137542"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VKL21_06250"
     CDS             complement(<1..465)
                     /locus_tag="VKL21_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HMB45405.1"
                     /translation="MKIKYKLILGFLIISMLAGIIAYYGMESNNSMKRAYGMTNDQTI
                     PVIKALEDIKFSSSRIEAKTYEIINSIPGNPNKLNESQEKYNELESEIESYNNSFNNY
                     RLLINDFFPEEKGVLEKIGNSGEKFIKESFNLIELRKQYVDESEFEKKKEEYE"
     gene            complement(462..1964)
                     /locus_tag="VKL21_06255"
     CDS             complement(462..1964)
                     /locus_tag="VKL21_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45406.1"
                     /translation="MVHENILVVEDEAIVAEDIKNVLLMEGYFVCATAPSGDAAIKKV
                     HEYHPDLILMDINLKGNMDGIETSGKIREQFDIPIIYLTAFSEKNTIDRAKLTNPMGY
                     IVKPINRRDLCIAIEITIYKHRADKKINEEKTWLNTTLKSISDAVVATDPQGRVKFMN
                     PVARSFSKCATAQFEGKSITELFDIRIKNPGTGSTGREAVETELVINNNLKIPIDIKS
                     ANIVDEIGNKKGIVMVFRDISEQKKNEMELKKINNELMKANEIKKQFLSVISHELRTP
                     LTPMKAQIQMILAGYLGEITDKQRPSFEMLNRNTLRLDRLIGDVLDISKLEAGVMKLN
                     MSKVDLYEIVKNVVETMKPQVEAKNIKLTLRGDRIPEIYADSDRFTQVVMNFIDNAFK
                     FTDNGGCIEVEISKDNDNAFIKVKDNGIGIKKEDQDNIFDPFVQIDSSYSRKHEGTGL
                     GLAICRGIVKFHGGKIWLESEFGKGCTFYISIPFEQCQDKNTELKILGGK"
     gene            complement(1969..2718)
                     /locus_tag="VKL21_06260"
     CDS             complement(1969..2718)
                     /locus_tag="VKL21_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45407.1"
                     /translation="MTTILVVEDEAIVAEDIKNSLQMAGYTVPATASSGEDAIKKVKE
                     FNPDIVLMDIVIKGEIDGIETAKRIRSGFDIPVVYLSAFSDEKTIERAKITEPFGYII
                     KPFNKRELSIAVEMALYKHKIEEDLKTSRKFFESILQSIVFGIWVTDKDDIIRYANQG
                     VGKVIGINPEKIIGMRIFNDFNEFIRPYYQKAKEEKQPFYFEAVPFNTSGCQSYKSGW
                     LIPRIINKNFDGMICTIESIPRYSIMDETRA"
     gene            complement(2715..5486)
                     /locus_tag="VKL21_06265"
     CDS             complement(2715..5486)
                     /locus_tag="VKL21_06265"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB45408.1"
                     /translation="MKKESVTNINHILKILLIVITIFFIGYFLESLRGISSMIGDYFL
                     ISTEILPLVLSFAIFIVTWYSYDKSKDNHWIFLGLACLVIGLIDLFHLLSYPFMPDFI
                     SANSTQKTTVFWGEARLVSALLFLASVYVTKNNFPDVINKTALFLSAIIISSIFFVIP
                     LLFPDNLPAMYRADGYPSGASIILVAVTILITLYAGSIYVDRTRQKDTEIIVFLKYGL
                     ITMVVSYPVYFFYGYSEHLLRAAGFYFLYLAIYKSSIEQPYEKLALADEKLLQETREK
                     YDNLFNNPHDAIITCHPDEIIVSWNKSAEKMFGWIADEAIGKGLPELIMLPENRDKID
                     RKINEVVSGRVVTGIDSTYIRKDGTKLDVNITISPIQGIDTKTIVLSYIIRDVTERKN
                     AENTLQASEKKYSTLVEKGNDGIVIIQDGNLKFVNSKIVQITGFTIEEGLGKPFVDFI
                     SEKFKETVIEIYRQRISGEVPKKNYEIEIISKTGKNIPVEINASLIEYEGRPAVMAIL
                     RDITERRQAQEALSWEIEENVAISELSSTLLSQATIEDISVLVLGHAKRITGSNYGYA
                     GYIDTKTGYLVCPSMSHDTMDVCKMKDSNNILKEFNGLFGWVLKNEKPLLTNSPSSDP
                     RSSGTPNGHIPIFRFISAPAVLKENLIGQISAGNPIGNKIHSIRYYDKGKLVGQVALA
                     NSDRDYTDRDLAFVERLTDIYAIAINRYLAQEKIKKSLKEKEVLLREIHHRVKNNMQI
                     ISSLLSLQSNSMPEEKYKGIFKESQNRIISMALIHEKLYHSRDIEKIDFKEYIEDLAK
                     SLFQSYEVYGNIELRIDVKDVFLGIDFAIPCGLILNELVTNSIKYGFPDGRKGMISIF
                     IKSNETGYVELEVNDNGVGIPEGFDISKTSSLGLHLVTILSEQLNGEISWDGNDGTKF
                     KIKFKEVIK"
     gene            complement(5642..6154)
                     /locus_tag="VKL21_06270"
     CDS             complement(5642..6154)
                     /locus_tag="VKL21_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45409.1"
                     /translation="MSQREELEKLAKACEECWGKDIASLDEHLERCPVCQEYKIKSEK
                     INQMMEAVHMFASKPEDERRKILGARMEQFSTMPEEKRIMAIGDMLDSISELSEEDRI
                     KIVRTRTDIITSLSEQKKEILMGSLKKVLAGWPEERKMMERQAVIAATQDYFILKRMI
                     VRKMFKKMLE"
     gene            6305..>6590
                     /locus_tag="VKL21_06275"
     CDS             6305..>6590
                     /locus_tag="VKL21_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMB45410.1"
                     /translation="MQVIKINNSPDDANAYLVNGTILIDVGMDCNDIIPELGKHIDLH
                     DLELIILTHCHYDHSGGAGGVAAATGARIAVHKNDAPLLLNPDASASRLFG"
BASE COUNT         1800 a         1385 c         1112 g         2293 t
ORIGIN      
        1 ttcatattcc tctttttttt tctcgaattc agattcatcg acatattgtt ttcttagctc
       61 gatcaaatta aagctttctt ttatgaattt ttcccctgaa tttcctattt tttcaagaac
      121 tcctttttct tccgggaaaa aatcgtttat tagaagcctg taattattaa atgaattatt
      181 ataagattca atttctgatt caagttcatt atatttttcc tgagattcat ttaatttatt
      241 tggattacct ggaattgaat ttataatttc atatgttttg gcttcaattc tcgaactgga
      301 aaatttgata tcttcaaggg ctttgataac aggaatagtc tgatcatttg tcatgccata
      361 tgcccttttc attgaattat ttgattccat cccgtaataa gcaatgatcc cggcaagcat
      421 agagattatc aggaagccta atatcaattt atatttaatt ttcatttacc acctaatatt
      481 tttaattcag tatttttatc ctggcattgt tcaaacggga tggagatata aaatgtgcaa
      541 ccttttccga actcgctttc aagccatatt ttcccgccgt ggaatttcac tataccccta
      601 caaatcgcca ggccaagccc cgttccctca tgttttctgg agtagcttga atctatctgg
      661 acaaaaggat cgaaaatatt atcctggtct tcctttttta ttccgattcc attatctttt
      721 acttttataa atgcgttgtc gttatctttt gaaatttcaa cttctataca gccgccatta
      781 tcagtgaact tgaaggcatt gtctatgaaa ttcatgacca cctgggtgaa cctgtcgctg
      841 tccgcatata tttcagggat cctgtctccc cttaatgtaa gcttaatatt ttttgcctca
      901 acctgaggct tcatggtttc aaccacattt ttcacaattt cataaaggtc aacttttgac
      961 atattgagtt tcatcacgcc agcttccagt tttgaaatgt caagtacatc ccctataagc
     1021 cggtccagcc tcagggtatt tcggtttagc atttcaaaac ttggtctttg tttatcggtg
     1081 atctcgccaa gataccccgc aaggatcatc tgtatctgcg ctttcattgg agtaagagga
     1141 gtccgcagct catgtgaaat aacgcttaag aattgcttct tgatttcatt tgctttcatg
     1201 agctcattat tgattttctt aagttccatc tcgtttttct tttgttctga gatatcccgg
     1261 aataccatta ctattccttt tttattccca atttcatcta cgatattggc gctctttata
     1321 tcgataggga ttttcagatt attatttatg accagttcag tttccacagc ttctctaccc
     1381 gtagaacctg ttccaggatt ttttatcctt atatcaaaaa gctcagtaat ggattttcct
     1441 tcaaattgcg cggttgcgca ttttgaaaaa gatcttgcga ccggattcat gaatttcacg
     1501 cggccctggg gatcagtagc gacaacggca tcgcttatac ttttaagggt ggtattaagc
     1561 caggtttttt cctcgtttat ttttttatct gccctgtgtt tataaatcgt tatctcgatc
     1621 gcgatgcaca gatcccttcg attgataggc ttaactatat aacccattgg attagtcagt
     1681 tttgccctat caatagtatt cttttctgaa aatgcagtaa ggtatatgat gggaatatca
     1741 aattgttccc gtattttccc tgatgtttcg atcccatcca tgttcccttt caggttaata
     1801 tccataagga taagatccgg atgatattca tgtactttct tgatcgctgc atcccctgaa
     1861 ggtgcagttg cgcatacaaa atatccttcc atcaggagaa cgttcttaat atcttcagct
     1921 acaatggcct catcttcgac aacaaggata ttttcatgaa ccattattct acgcccgggt
     1981 ttcatccata atgctatatc gggggatact ctcgatcgtg catatcatac cgtcaaaatt
     2041 tttatttatt atcctgggta ttaaccagcc tgatttatag ctctggcatc cgcttgtatt
     2101 aaaagggaca gcttcaaaat agaaaggctg tttttcttct ttggcttttt gataataggg
     2161 ccttatgaat tcattgaaat cattgaatat ccgcattcca atgatctttt ccggatttat
     2221 gccaataact ttgccaactc cctggttggc atacctgata atatcatcct tatccgttac
     2281 ccatatacca aaaacaatgc tctggagaat gctttcaaag aatttcctgc ttgttttcaa
     2341 atcctcctct atcttatgtt tataaagcgc catttcgact gcaatgctca attccctttt
     2401 attgaatggt ttgatgatgt atccgaaagg ctctgtaatt tttgcccttt caattgtttt
     2461 ttcatctgaa aatgcactca ggtacacaac agggatatca aaaccggaac gtatccgttt
     2521 cgcagtttca atgccatcta tctccccttt gatcacgata tccattaaca ctatatctgg
     2581 attgaattct ttcactttct tgatggcatc ttccccggaa gatgcagttg caggaacagt
     2641 atatcctgcc atctgcaagc tgttctttat atcttctgct acaatagctt catcttcaac
     2701 aaccagtatt gtcgtcattt tattacctct ttgaatttga tcttaaattt agtaccgtca
     2761 ttcccatccc agctgatctc cccattaagc tgctcagaaa gtattgtgac aaggtgcagg
     2821 cccagcgatg aggtcttgct gatatcaaaa ccctcaggaa tgccaacgcc attgtcattg
     2881 acctcaagtt caacataacc agtctcattt gatttaatga atatgctgat catacctttt
     2941 cttccgtccg gaaaaccata ttttattgaa tttgtaacaa gctcgtttaa tatcagtcca
     3001 catgggatcg cgaaatctat gcccagaaaa acatccttta catcgattct cagttcgatg
     3061 ttgccatata cctcatatga ctggaaaaga ctctttgcaa ggtcttcaat atattcctta
     3121 aaatcaatct tttcaatatc cctggaatga tacagtttct catgaataag agccattgag
     3181 attatccggt tctggctctc tttgaatatt cccttatatt tttcttcagg catggaattt
     3241 gactggaggc tcagcaggct tgagatgatc tgcatgttat tctttaccct gtgatggatc
     3301 tcccttagaa ggacttcttt ttctttcaat gatttcttta tcttctcctg ggctaagtag
     3361 cgattaatgg caattgcata aatgtcggtg aggcgttcaa cgaatgcaag gtcgcggtca
     3421 gtgtaatccc gatctgaatt agccaatgcc acctgaccca cgagttttcc tttatcataa
     3481 tatcttatcg aatggatctt gttcccgatt ggatttccgg cggatatctg gccaataaga
     3541 ttttctttaa gcacagcggg agctgaaata aaacgaaaaa tgggaatgtg gccattcggg
     3601 gttcctgatg accgtgggtc gctggaagga gaattggtaa ggagcggctt ttcatttttt
     3661 aatacccagc cgaaaagtcc gttgaattct ttcaggatgt tattactatc cttcattttg
     3721 cagacatcca tagtatcgtg ggacatgctg ggacaaacaa gatagcccgt tttcgtatca
     3781 atatatcccg cataaccgta gttgcttcct gttattcgtt ttgcgtgacc aagcaccaga
     3841 acagaaatat cttcgatagt agcctgggat aaaagcgtgc tggataactc tgaaattgct
     3901 acgttttctt ctatttccca tgacagagct tcctgtgcct gccgcctttc tgttatatcc
     3961 cgcagtatcg ccataacggc tggcctgcct tcatattcta tcaacgaggc atttatttca
     4021 accgggatat tttttcctgt ctttgaaatg atctctattt cataattttt tttagggact
     4081 tctcctgata tcctctgtct gtatatttct attactgttt ccttgaattt ctctgatatg
     4141 aaatcaacaa atggttttcc gagtccttcc tcaatagtga atccggtaat ctggactatc
     4201 tttgagttca cgaatttcag attgccatcc tgaattatga caattccatc atttcctttc
     4261 tcaacaagtg tggagtattt tttctcactt gcctgcaatg tattttctgc gttcttacgt
     4321 tctgtaacat ccctaattat atatgaaagt acaattgtct ttgtatctat gccctgaatc
     4381 ggggaaattg ttatgtttac atcaagtttt gtaccatctt ttcggatgta tgtcgaatct
     4441 attccagtaa caactcttcc ggatacaact tcattgattt tcctgtctat tttgtccctg
     4501 ttctcaggca gcatgattaa ttcggggagc ccttttccga ttgcttcatc tgcaatccat
     4561 ccgaacatct tttctgcgct tttattccat gaaacaatga tttcatccgg atgacatgtg
     4621 atgatagcat catgtggatt attgaagaga ttatcatatt tctccctggt ttcctggagg
     4681 agtttttcat ccgccagcgc cagtttttca tatggctgtt cgatagatga tttataaatg
     4741 gcaagataca ggaaataaaa acctgccgct cttaacagat gttcagaata cccataaaag
     4801 aaataaacag ggtaactgac taccatcgta attaaaccat atttcaaaaa aacaatgatt
     4861 tcagtatctt tttgccttgt tctgtctaca taaattgaac cggcatataa tgttatcaat
     4921 atagttactg caactaaaat aatacttgcg ccggacggat acccatcagc cctatacata
     4981 gctggaagat tatctggaaa taataaaggg ataacgaaaa atatacttga tattattatt
     5041 gcagacaaaa atagcgctgt tttgttgata acatcaggaa aattgttctt ggtaacatat
     5101 acgcttgcga ggaaaaggag ggcagaaaca agacgcgcct caccccagaa gaccgtggtc
     5161 ttttgagtgg aatttgcaga aatgaaatcc ggcatgaaag ggtatgaaag caaatggaac
     5221 aggtcaatta atccgatgac aagacatgcc aaaccaagaa aaatccagtg attatcttta
     5281 cttttgtcat atgaatacca tgtaactatg aagatcgcaa aagaaagaac aagcggcaga
     5341 atttcggttg atattaaaaa ataatcgcct atcattgaag aaatgcccct caatgattcc
     5401 aggaaatatc caatgaaaaa aatcgtgatt actattaata aaatttttaa aatatgatta
     5461 atatttgtca cactttcttt tttcattatt atcatagtga taatgatggt gtaaatagct
     5521 tttcatataa tactttctta tagagtcaaa atgcatggtt gcgagtgagt ttattgaaaa
     5581 ggagatgtca aaatgaaagc attaaagata ggtcaagagc tattccttcc agaacatggg
     5641 attattccag catctttttg aacatcttcc tgacaatcat ccgtttcagt atgaaataat
     5701 cctgggtggc cgcaattaca gcctgtcttt ccatcatctt tctttcttct ggccatcccg
     5761 ccaatacttt ctttaagctc cccatcagga tttccttttt ttgttccgat aaacttgtga
     5821 taatatctgt tcttgttctt actatcttta tcctgtcttc ttcagagagt tctgatattg
     5881 aatcgagcat atcccctatt gccataattc ttttctcttc aggcatggtt gagaactgct
     5941 ccattcgggc acccaatatc tttcgccttt catcctcagg ctttgacgca aacatatgta
     6001 cagcttccat catctgattg atcttttcag attttatctt atattcctgg catacaggac
     6061 acctttcgag atgttcatcc agtgatgcta tatctttacc ccaacactct tcacatgctt
     6121 ttgccaactt ttccaactct tctctctggc tcataaaaaa aaccacctgt taaaaataat
     6181 aggcattttc ttagttaaat agttacttga gattttcctg agacttttta ttatcttctg
     6241 gttattatgg cagctttcct ttattgcaaa gttataaatg aattcacaat atatgtgctc
     6301 taccatgcaa gttattaaaa taaataattc tcccgatgat gccaatgcat acctcgtaaa
     6361 tggaacaata ctcatagatg ttggaatgga ttgcaacgat attatccctg agcttggaaa
     6421 acacatagac ctgcacgatc tggaactaat catacttaca cattgccact atgatcattc
     6481 aggaggagca ggaggtgttg ctgcagcaac aggcgcaaga atagctgttc ataaaaatga
     6541 tgctccactt cttttgaatc cagatgcgag tgcttcacgc ttattcggta
//
LOCUS       DATGBN010000259         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_292222, whole genome shotgun sequence.
ACCESSION   DATGBN010000259 DATGBN010000000
VERSION     DATGBN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292222"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VKL21_06280"
     CDS             <1..258
                     /locus_tag="VKL21_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45411.1"
                     /translation="TIIEKYHDRIVSIHMKDKTGPKTDPANTNQVWGQGEAPVADILL
                     LIKQKGWPIHCDIELEYDVKPWSNARKEVKTCVQYARQILM"
     gene            complement(291..893)
                     /locus_tag="VKL21_06285"
     CDS             complement(291..893)
                     /locus_tag="VKL21_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HMB45412.1"
                     /translation="MNTKNPIKTRSSAKDFPVVCVGGSAGGLDSYTRLLLNLPSDMGV
                     AIVIVNHLRTVATRLHEILPKYTDMPVELITERLDIKPNHVFIIPEKRDLHVLNDEFR
                     LKPISKPRGWPDVITVFLRSLTQNWDGKLIAVIVSGYDGDGAEALCGIKAVGGITIAQ
                     KPETAEMPDMPNSAIASGCIDFILSPEDIAKQIVRIAHSE"
     gene            complement(933..>1652)
                     /locus_tag="VKL21_06290"
     CDS             complement(933..>1652)
                     /locus_tag="VKL21_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMB45413.1"
                     /translation="NVNKELAALSHDLQSPLAGIIELAEILKENFETFENSTVKEMLE
                     LIYKSSKDELIMLDYFVEMARIKYASEAFSPAKTDLNQYVEKVFNTLTENAVAKNIHL
                     YNEIEENTNVFADGKMLLSILQNIISNSIKHTRSGGKIAITAKRKEDKIIIEIKDNGI
                     VTSEEKTKPLFTPQMISLSNIKKENNGAGIGLLFAKIFLEKNGGEIWVESIKGEGSSI
                     YLTLPLKQTINKKDNQTKFNS"
BASE COUNT          480 a          326 c          296 g          550 t
ORIGIN      
        1 acgattatag aaaaatatca cgatcgtatt gtcagtatcc atatgaagga taagaccggt
       61 ccaaagactg atccggccaa tacaaatcag gtctggggtc agggtgaagc accggttgcc
      121 gatattcttc tgctgataaa acagaaagga tggccgatcc attgcgatat tgagctggaa
      181 tatgatgtta aaccatggtc aaatgccaga aaagaggtta agacctgtgt tcagtatgca
      241 agacagatcc ttatgtagac tttgatcatc ttaagtaagt tccttctgct ttactcagaa
      301 tgcgcaattc gaacaatttg tttagcgata tcttcgggtg aaagaataaa atctatacac
      361 ccgcttgcta ttgcgctatt tggcatatcc ggcatttcgg ctgtctccgg tttctgtgcg
      421 atggtaattc cccccacagc ttttataccg catagtgctt ctgccccatc cccatcatag
      481 ccagaaacaa taacagcgat tagttttccg tcccagtttt gtgtcagaga acgaagaaaa
      541 acagtaatca catcgggcca tcctctgggt tttgatatcg gtttcagccg gaactcatca
      601 ttaagaacgt gcaaatcgcg cttttcagga atgataaaca catgattagg tttgatatcc
      661 agtctttcag tgataagttc aacaggcata tcagtatatt tcgggagtat ttcgtgaagc
      721 cgcgtggcca cagttctcag gtgattcacg atgacaatag caacccccat atcgcttggc
      781 aaattcagta aaagacgggt ataagagtcg agtccacctg ccgaaccgcc cacgcaaact
      841 accggaaagt cttttgcaga actacgggtt ttaataggat tcttagtatt cattgataat
      901 caaacttaac aaggtcataa ttaaaaagct ttttacgaat taaattttgt ctggttatcc
      961 tttttattta ttgtttgttt caaaggtaat gtcaaataaa tggatgagcc ctcaccttta
     1021 atactttcta cccaaatttc accgccattt ttttccaaaa atatttttgc aaacagtaat
     1081 ccaataccgg ctccattatt ttctttctta atatttgaaa gagaaatcat ttgtggagta
     1141 aaaagtggct tcgttttttc ttcggacgtt acaatgccat tatcttttat ctcaataatt
     1201 attttatcct cttttctttt cgctgtaatt gctattttcc ctccggaccg agtatgtttt
     1261 attgaatttg aaattatgtt ttgaagtata gaaagcaaca tttttccatc tgcaaaaaca
     1321 tttgtattct cttcaatttc gttataaaga tgtatgtttt ttgcgacagc attttcagtt
     1381 aaagtattaa aaactttctc aacatattga ttaaggtcag tttttgcagg tgaaaatgct
     1441 tccgaagcgt attttatcct tgccatttcc acaaagtaat caagcataat taactcatct
     1501 tttgatgatt tatatatgag ctcgagcatt tcttttacgg tactgttctc aaatgtttca
     1561 aaattttctt tcaatatttc agccaattca ataatgccgg ccaaaggact ttgcaaatcg
     1621 tgtgagagtg cggcaagctc tttatttaca tt
//
LOCUS       DATGBN010000260         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_331681, whole genome shotgun sequence.
ACCESSION   DATGBN010000260 DATGBN010000000
VERSION     DATGBN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331681"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VKL21_06295"
     CDS             <1..239
                     /locus_tag="VKL21_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMB45414.1"
                     /translation="LPSIMTGLRIGFGVGWMAIVAAEMVIRSPVGLGYFIWNMGDLGR
                     YSEMVAGMIVIGVIGYIMNISILLLQRRFIKWGD"
     gene            227..985
                     /locus_tag="VKL21_06300"
     CDS             227..985
                     /locus_tag="VKL21_06300"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB45415.1"
                     /translation="MGRLVINSVSKKFNDFLALENVNFEVADGEFVCLLGPSGCGKTT
                     VLRLAAGLEEPDDGFLCLDGKKISGVNKECGFVFQEYALFPWRTVKENIEFGPQVKGK
                     TSLECESISRHYIDLVGLGGFENHYPNELSGGMKQRVGIARAYANDPKLLLMDEPFGA
                     LDAQTRNLMQSELLRIWEKEHISVLFVTHSVDEAVYLGDKVVIMSAHPGKIKETFEVK
                     LTRPRIRTGPEANIIRDRVLKSLVSEIKIASKPG"
     gene            complement(1013..1342)
                     /locus_tag="VKL21_06305"
     CDS             complement(1013..1342)
                     /locus_tag="VKL21_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45416.1"
                     /translation="MRKVKADDLKEHDEICRKKEESKMGIWAWIQPIDRIWKVISDTE
                     KGTLYVYNEKNELILEQKGMTKEEVSFIEDNFLQIVAKNLNEEKIVPNHIVNVERPVH
                     DFNYMYA"
     gene            complement(1477..1983)
                     /locus_tag="VKL21_06310"
     CDS             complement(1477..1983)
                     /locus_tag="VKL21_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45417.1"
                     /translation="MKLEEIIKQIANVSKAEIKKIAPEDCNEIKDLILESEPGSLYEK
                     MVLGYLTSICAECMYPEIFHLSRNDLDYIGFELEKGTIEVEIAGNMPGACMKGGKIEI
                     KKAGDETGSSMNGGEIIADEIKSIGNTIKGKIIVRQVGKISKNQGSQIFIDGVKFKRN
                     FFDRLLGK"
     gene            complement(1986..2384)
                     /locus_tag="VKL21_06315"
     CDS             complement(1986..2384)
                     /locus_tag="VKL21_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HMB45418.1"
                     /translation="MKIARETLQFILEVCKSSAPLEFAGMLSARGDVITEVIVVPGTE
                     SSDESAVMQLFMLPNIHTIGSVHSHPSGNITPSGVDLELFSKKGMVHIIVGAPFDSRS
                     WRCYDKDGVPVKLEVVDYEFKEENDFVSDE"
     gene            2444..2517
                     /locus_tag="VKL21_06320"
     tRNA            2444..2517
                     /locus_tag="VKL21_06320"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2478..2480,aa:Lys,seq:ctt)
     gene            2737..3717
                     /locus_tag="VKL21_06325"
     CDS             2737..3717
                     /locus_tag="VKL21_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45419.1"
                     /translation="MINEETINQAIFDYADKKYGKRSKEMLSGYINEFPEKDWDLPDE
                     LWSNNFLAWLFFEKVFPETGMTIAEEFAKDSHDLSPEMKDNVLQMKNMIRSKFIVISK
                     KDLFLKMKDMKTNKVYKVKLPAPSPVSPNNMITGRIHPFGDHYCFSSVFLITTSPLIL
                     DPDVLMSAYENEGLKKIESITLRKGSSLQSIMNKYPAHWIDWMCKHYGLKERLKTEKV
                     RGIENKIVTDLPQIVSKLPEKSREALAFCIKEGGFVKYGQLKDFDDDMDFFWKEGKTF
                     STIGTLRQKGLMVVGKMVFGDRQFKVAFVPVEIRDGLKSILSAKISEMEK"
     gene            3734..4267
                     /locus_tag="VKL21_06330"
     CDS             3734..4267
                     /locus_tag="VKL21_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMB45420.1"
                     /translation="METDINKIRKLSIEKEDEKWEFRSSLKGCDISEERIDLIVHELY
                     KKVSSEIDCRTCANCCREVQPVLDQEDIERLSKCLGISVAQFKDQHLVKDEDPGKFSF
                     NKKPCPFLKDNLCSHYTYRPKDCKSYPHLHKCGFIFRLINVIGNCSICPVVFNVYEYL
                     KDEIQNDDDSNNFNGFD"
     gene            4396..4536
                     /locus_tag="VKL21_06335"
     CDS             4396..4536
                     /locus_tag="VKL21_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45421.1"
                     /translation="MDSIHALAKVYDSLSKGTSTLDFPPSDITGDCAAQVQEIVRKRK
                     PK"
BASE COUNT         1392 a          893 c          979 g         1408 t
ORIGIN      
        1 ctcttccatc aattatgacc ggcttgagga ttgggtttgg tgtggggtgg atggcgatcg
       61 ttgcagccga gatggttatc aggagtcctg tgggcctggg ctattttatc tggaacatgg
      121 gggacctggg gcgctattcg gaaatggtgg caggtatgat tgtcataggc gtgatcggat
      181 atattatgaa cattagcatc cttcttttgc agaggaggtt cataaaatgg ggagattagt
      241 cataaattcg gtgtccaaga aattcaatga ttttcttgcg cttgaaaatg taaattttga
      301 agttgcagat ggggagttcg tgtgccttct tggccccagc gggtgcggaa aaacaacagt
      361 ccttcgcctt gccgcagggc tggaagagcc ggatgatggc tttttatgcc tggatggcaa
      421 gaaaatatcc ggggtcaata aagaatgtgg ttttgtcttc caggaatatg ctctgttccc
      481 atggaggacc gtgaaagaaa atatcgagtt cggacctcag gtaaagggaa agacaagttt
      541 ggaatgtgaa agtatctcca gacactatat agacctcgtg ggtctgggcg gatttgagaa
      601 ccattacccg aatgaattat cagggggtat gaaacagagg gtcgggatcg ccagggctta
      661 tgcgaacgac ccgaagctgc tattgatgga tgaacctttc ggggcgcttg atgcccagac
      721 aagaaatctc atgcagagtg aattgctaag aatctgggag aaagaacaca taagtgtgct
      781 ttttgtaact cacagcgtgg acgaagctgt ttaccttgga gataaggttg ttatcatgtc
      841 agcccatccg ggtaagatca aagagacatt tgaagtcaaa ttgactcgcc caaggatacg
      901 cacgggccct gaagccaata taataaggga cagggttttg aaatcacttg tcagtgagat
      961 aaaaatagca tcaaaacccg gataatacaa tcgtttttac caggaaaaac actcacgcat
     1021 acatgtaatt gaagtcatgc actggccttt ccacattaac gatatggttg gggactattt
     1081 tttcctcgtt gagatttttc gcgactattt gaaggaaatt atcctctatg aaagagactt
     1141 cttcttttgt cattcctttt tgttccagga tcaattcatt cttttcatta tatacataaa
     1201 gggttccttt ttcagtatca gaaattacct tccatatcct atcaatcggt tgaatccatg
     1261 cccatattcc catttttgat tcctcctttt ttctgcatat ttcatcatgt tccttgaggt
     1321 cgtccgcctt tactttcctc aattcatccg tgagcccgat aggtcacgag tttgacacac
     1381 atatcttagt ttagttatat tcttatttaa atttatctat taaaatttta ataattatga
     1441 taataataat tagtctaact ttttagaggg gatggtttat ttccccaata gcctgtcaaa
     1501 aaaattccgc ttgaatttaa ccccatctat aaaaatctgt gagccctgat tttttgagat
     1561 cttaccgacc tgtcttacaa ttatttttcc ttttatggta ttcccgatgc tcttgatttc
     1621 atccgcgatt atctctccgc cgttcatgga gcttccggtt tcatccccgg ctttctttat
     1681 ttcgatcttt ccccctttca tacaggcccc aggcatgttt ccagcgatct caacttcaat
     1741 cgtgcccttt tcgagttcaa aaccgatata atccaggtca ttgcgagaga ggtgaaatat
     1801 ctccgggtac atgcattctg cacatatcga tgtaaggtag ccgagtacca ttttctcata
     1861 caaactgccc ggttctgatt ccagtatcag gtctttgatc tcattacaat cttcgggcgc
     1921 gatctttttt atctcagctt tactgacatt agcaatttgt ttgatgattt cttcaagttt
     1981 cataattatt catccgatac aaagtcattt tcttctttga actcataatc cacgacttcg
     2041 agtttgaccg gtaccccgtc tttatcatag catctccagc ttctggaatc aaaaggtgcg
     2101 cctacgataa tatggaccat tcccttctta ctgaagagtt caagatcaac gccagatgga
     2161 gtaatattcc cggatggatg gctgtgcact gaccctatag tatgtatgtt gggaagcatg
     2221 aataactgca taacagcgct ttcatcgctt gactccgtgc ccgggaccac tataacttca
     2281 gttatgacat ccccccgggc agaaagcatt cctgcgaatt caaggggagc ggatgatttg
     2341 catacttcaa gtatgaattg taaagtttct cgtgcgatct tcatgttata cctgattggt
     2401 gcgaaatgta tatattaact tagcattatt agaaggaagt cttggactgg taacttagac
     2461 aggcagagcg tcggactctt aatccgacgg ccgtgggttc aaatccctcc cagcccgtat
     2521 aaatataaat taaaatcgat catttttttg gatctttctt aaacggcaag aagagattac
     2581 tattagcgta ctattaaaac tttcgttcaa ccttagcttt atagcgctgg ggttgcgacc
     2641 ccagacccca acggttgcgc cgctgctggc ggggtttagt atctgcattc ctggatgagt
     2701 tgaagaacta tgaaaaaagg gagcaatatg atttagatga tcaatgaaga gaccattaac
     2761 caggcaattt ttgactatgc agataaaaaa tatggcaagc gatcaaaaga aatgttatcg
     2821 gggtatatta atgaatttcc cgaaaaagac tgggatctgc ctgatgaact ttggagcaac
     2881 aattttcttg catggctttt ttttgaaaaa gtgtttccag aaacaggcat gacaattgca
     2941 gaagaatttg caaaagactc tcatgatttg agtcctgaaa tgaaagacaa tgttctacaa
     3001 atgaagaaca tgatccgatc caagtttatc gttatttcaa aaaaggattt gttcttaaag
     3061 atgaaggata tgaagacaaa caaagtatat aaagtaaaat tacctgctcc aagccccgta
     3121 tctccaaaca atatgataac tggcaggatc catccatttg gcgatcatta ctgtttctca
     3181 agcgtatttc tgattacgac atcgccactt atattggatc cggatgttct tatgagtgca
     3241 tatgaaaatg aagggcttaa gaagatcgag agcattacat tacgcaaggg ttcttccctg
     3301 cagtcaataa tgaataaata tccagctcac tggatagatt ggatgtgcaa gcattatggt
     3361 ttgaaggaga gacttaaaac tgaaaaagtc agggggatcg agaataaaat tgtaactgat
     3421 ctaccccaga tcgtttcaaa attacctgaa aagtccaggg aagcgcttgc tttttgtatc
     3481 aaagagggcg ggtttgtgaa atatggccag ctcaaagact tcgatgatga tatggatttc
     3541 ttctggaagg agggaaaaac cttttccaca attggcacgc tgaggcagaa aggactgatg
     3601 gttgttggaa aaatggtctt tggtgacaga caatttaagg tcgcgtttgt acctgttgaa
     3661 atccgtgatg gtttaaaatc tatactatcc gctaaaattt cggaaatgga aaaatgatga
     3721 acaaataatc tatatggaaa ctgatataaa taaaattagg aaattatcaa tagaaaaaga
     3781 agatgagaag tgggaattcc gatcttcctt aaaaggctgt gatatttcag aagagagaat
     3841 agatttaatt gtacatgaat tatataagaa agtttcttca gaaatagatt gtagaacttg
     3901 tgctaattgt tgcagggaag tgcaaccagt tttagatcaa gaagatattg agagactctc
     3961 caaatgttta ggcatctctg ttgctcaatt caaagaccaa catttggtta aggacgaaga
     4021 tccgggaaaa tttagtttta acaagaagcc atgtccattt ctaaaagata atttatgttc
     4081 acattacact tatcgtccta aagattgtaa atcttatcca catctacata agtgtggttt
     4141 catttttaga ttgatcaatg taataggaaa ttgttccatt tgccctgtcg tatttaatgt
     4201 ctatgaatat ctgaaggatg aaattcagaa tgatgatgac tctaataatt ttaacggttt
     4261 tgattgagct tatggaaaat aacaggctat ttaacaaaaa tcagcgaaac gattgaaata
     4321 tcaaagccat gaacctcaca aataacatct tttaaaaaaa aattagatat ctctatactc
     4381 ctatactcaa tcataatgga ctccatccac gcccttgcaa aagtctatga ttccctctca
     4441 aaaggcacat ccacgcttga tttcccgcct tctgacatca caggagactg cgccgcgcag
     4501 gttcaggaga tcgtgaggaa gaggaagcca aaataagaga aaacgacatg catgatcctg
     4561 attatcacag cggcaaaacc gaaataaagc gctaggctcc acgcctaaag gtatatattt
     4621 tgtgtcgcat gcgagatttt catggttgat ttagtttctt accccacctt ct
//
LOCUS       DATGBN010000261         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_448067, whole genome shotgun sequence.
ACCESSION   DATGBN010000261 DATGBN010000000
VERSION     DATGBN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448067"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VKL21_06340"
     CDS             complement(<1..1092)
                     /locus_tag="VKL21_06340"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HMB45422.1"
                     /translation="MKILIIGGTGETGRWFARFYKKHGFDVVIWGINKKKEIAEELGV
                     AFANDIDSEIRSSDIVMISVPINKTEKTISDIAPKMSSGSLIMDITSIKTGPMETMVK
                     YAPEDVEVLGSHPMFGPSIPDMRGQIVIFTPVRGSKWFPIIKSLYEDNGAHIEILEAL
                     EHDRMMAVVQGLTHFAYITIGTVFRELEFDVAKSRRFMSPMYDIMLDLVGRILAQNPY
                     LYAMIQMNPEVAKVHKAYIDQCNLMADIVKRKDIEGFAGLMKKAAAQFGDTESALRRS
                     DKLIGTKIAEHEELVRSIGNQRALKHIYSGVVHIGIVKKVTPRTVILERGGKNIEFLI
                     ENVRPLNEKELFSWKSSNMKNVTRDVSVYI"
     gene            complement(1089..1904)
                     /gene="aroE"
                     /locus_tag="VKL21_06345"
     CDS             complement(1089..1904)
                     /gene="aroE"
                     /locus_tag="VKL21_06345"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMB45423.1"
                     /translation="MKLFGVLGDPVSHSLSPAMHNAAFKALGMDCEYHAFRVSADSLH
                     DAIHGACALGFGGLNLTIPLKEKALEIVKPTDLAKQIGAVNTIDFKNGIVGHNTDGLG
                     ARMALTESDVEIKGKNVLLLGAGGAARAIAFTLATEGAIVTIANRTPERAFSLAKEVG
                     RAGKISASGLEDLKNLVKNSDILINSTAIGMFPGVSETLVTSDMMHRDLVVFDIVYNP
                     ISTRLLQEAKIAGVKTIDGVMMLVYQGAGAFKIWTGKTPPVDIMEKAVRERLL"
     gene            complement(1957..2127)
                     /locus_tag="VKL21_06350"
     CDS             complement(1957..2127)
                     /locus_tag="VKL21_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HMB45424.1"
                     /translation="MTKKKEGSGLMSSAGLMRYYDSEETAVLIDPKTVVTVAILVGLA
                     VLISNATYGIWP"
     gene            2244..2765
                     /locus_tag="VKL21_06355"
     CDS             2244..2765
                     /locus_tag="VKL21_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45425.1"
                     /translation="MTIKNFIVDDYAAEGLPMRLVVTVILLSVIIGLSAKAVSLFLND
                     ANEKKLKGELDLIDKRAGMIYMQGGARDIDDPGDLSGTMEKIHVEIPDNAAFVVFGGM
                     PSRDGNPWAVGNVHTDNVFYYVLNDGRVQTRSSIARFFSNSPGQPFVLHPGEYDLTME
                     LVKNKNGTYVKIE"
     gene            2749..>3153
                     /locus_tag="VKL21_06360"
     CDS             2749..>3153
                     /locus_tag="VKL21_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45426.1"
                     /translation="MSRSNKTATFTQNESGWADFFMTRIGLIMFAAILLLTAFEIYPM
                     FQERESRLYLDIVASDIASKIEAVDSMTIPGYKYNYAFEENNRDIKIEISTEYVIAHT
                     NLGSSLWGERELIHPEPVITHVFPPNSNWSNTS"
BASE COUNT          884 a          737 c          591 g          941 t
ORIGIN      
        1 gatataaaca gagacatcgc gcgtcacatt cttcatattt gatgatttcc atgaaaataa
       61 ctccttttca tttaagggcc tgacattctc gataaggaat tcgatatttt tccctccccg
      121 ctccagaata acagttcgag gggtgacttt tttgacaatc ccaatatgga caacccctga
      181 atatatatgc ttgagtgcac gctgattacc tatggaacgc acaagttcct catgttctgc
      241 gattttggtg ccaatgagct tgtccgaacg acgaagtgct gattccgtat cgccaaattg
      301 tgctgccgct tttttcataa gacccgcaaa accttctata tccttccttt ttactatatc
      361 cgccatcagg ttgcactggt cgatgtaagc cttatgcact tttgccactt cgggattcat
      421 ctggatcata gcatacaggt acggattctg cgccaatatc ctacctacta agtcaagcat
      481 gatatcatac attgggctca tgaaccttcg tgattttgca acgtcgaatt caagctccct
      541 gaaaaccgtt cctattgtaa tataagcaaa atgtgtaaga ccctggacaa ccgccatcat
      601 cctgtcatgt tcaagcgcct ccaatatctc tatatgcgcg ccattatcct catacaggct
      661 ttttattatc gggaaccatt ttgaaccccg aacaggtgtg aaaattacta tctgccctct
      721 catatctgga attgaaggac caaacatggg gtgtgacccc agtacctcaa catcctctgg
      781 cgcatacttt accatcgttt ccatcgggcc tgtttttatt gaagtgatat ccataataag
      841 actccccgag gacattttgg gagcaatatc cgatatcgtt ttttcagtct tattaatagg
      901 aactgaaatc attacaatat cacttgatct gatttcactg tcaatgtcat tcgcaaaagc
      961 tacgcccaat tcttctgcaa tttctttttt cttatttatg ccccatatga ccacatcaaa
     1021 cccatgtttt ttataaaatc tggcaaacca tctgcctgtc tctcccgttc ccccgattat
     1081 taatattttc ataaaagtct ctccctgaca gctttttcca ttatatcaac aggaggggtc
     1141 tttcctgtcc atatcttaaa cgccccagct ccctggtaaa ccagcatcat cactccatct
     1201 attgtcttta ctcctgcaat ttttgcttcc tgaagcagtc gcgtactgat cggattatag
     1261 actatatcaa atactacaag gtccctatgc atcatatctg aggtaacaag ggtttcggat
     1321 actccgggaa acattccaat agcagttgaa ttaataagaa tatcactgtt cttgacaaga
     1381 ttcttaagat cctccagacc tgatgcactt attttccccg ctctccccac ctcttttgca
     1441 agagaaaaag ccctctctgg tgtcctgtta gcaatggtca caattgctcc ttcagtagca
     1501 agagtgaatg cgatcgctct tgctgcgcca cctgcgccaa gtagcaatac attctttcct
     1561 tttatctcca catcactttc agtgagcgcc atccttgccc ccagtccatc agtgttatga
     1621 ccaacaattc catttttgaa atcaatagta tttacggcgc ctatctgttt tgcaaggtct
     1681 gtgggcttga caatttcaag ggctttttcc tttagcggaa ttgtaagatt taagccgcca
     1741 aacccaagtg cacaggcgcc atggatcgca tcgtgaagag aatctgcgct gaccctgaac
     1801 gcatgatatt cgcagtccat gccaagtgcc ttaaatgccg cattatgcat tgcgggagaa
     1861 agactgtgcg atacaggatc gccgagaacg ccgaatagct tcatgctatc ctaattaaaa
     1921 gtaatggtta aaaaatatta tgattgataa tcagaattat ggccagatac catatgttgc
     1981 attagatata aggactgcca gtcctactaa tatcgccact gtaacaacag ttttcggatc
     2041 gatcaataca gcagtttctt cagaatcata gtatctcatt aagccggctg aagacatcaa
     2101 tccgctgcct tctttctttt ttgtcatgat aagtactaat gtttactata tgattttaat
     2161 gtatcgtaag aaaaacaaaa attccttata gtactatgct attttggcgg ctaaatcatt
     2221 aaaaaaaatg atagaaatcc cttatgacca ttaaaaattt cattgttgat gactatgccg
     2281 ccgaaggcct gccaatgcga cttgtagtta ctgttattct tttatccgtc attatcggcc
     2341 tgtcagcaaa ggctgtatct ctttttttaa acgatgcaaa tgaaaaaaag ctcaaaggcg
     2401 agcttgatct tatcgataag cgcgctggta tgatatatat gcagggagga gcgcgggata
     2461 tcgatgatcc tggggattta tcgggaacaa tggaaaaaat ccatgtggaa attccggata
     2521 atgccgcttt tgtggtcttc ggtggaatgc catcccggga tggtaatccc tgggctgtgg
     2581 gaaatgttca caccgataac gtattttatt atgttctaaa tgacggccgc gtgcaaacca
     2641 gatcatctat cgcgcgtttt ttttcaaaca gcccgggaca acccttcgtg cttcatcccg
     2701 gggagtatga cctaactatg gaacttgtca aaaacaaaaa tggtacctat gtcaagatcg
     2761 aataaaaccg ccacattcac acaaaatgaa agcggatggg ctgatttttt tatgaccagg
     2821 atcggattga tcatgttcgc agcgatatta cttcttacag cttttgaaat atatccgatg
     2881 ttccaggagc gggagagcag gttatatctc gatatcgtgg cctcggatat cgcatcaaaa
     2941 atagaagcgg ttgacagtat gaccatacca ggatacaaat ataactatgc tttcgaagag
     3001 aataacaggg atataaaaat agaaatctcc actgaatacg tcattgccca tacgaatctt
     3061 ggctcatccc tgtggggaga acgggaatta attcatcccg aacccgtgat aacgcatgtt
     3121 tttcccccaa acagcaattg gagtaataca tcc
//
LOCUS       DATGBN010000262         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_331817, whole genome shotgun sequence.
ACCESSION   DATGBN010000262 DATGBN010000000
VERSION     DATGBN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331817"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /gene="rtcA"
                     /locus_tag="VKL21_06365"
     CDS             complement(<1..834)
                     /gene="rtcA"
                     /locus_tag="VKL21_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HMB45427.1"
                     /translation="MKIDGSYGEGGGQILRTCVALSAVSGKPVEIENIRKGRPKPGLS
                     AQHVKAVEGLARICEASVSGCHLGSTRLKFTPGKIHGGNYEIDIGTAGSISLFLQCLM
                     PALMNAPGTVRIKITGGTDVRWSPSIDYMRFVTLFALRMMGYECELRLIRRGYYPRGG
                     GCVEAIINPSNLKKINFDSNKRSQVEGISHSSGLPSHVSQRQAASAQKLLLEHGYTSR
                     ISTEVNDYPSTGSGITLWCGTIGGTALGKPGLEAEKVGDIAARALLDDLGSGVDEFLQ
                     DQ"
     gene            complement(1012..1644)
                     /locus_tag="VKL21_06370"
     CDS             complement(1012..1644)
                     /locus_tag="VKL21_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HMB45428.1"
                     /translation="MNIEFGARTREAKEINEKSWSIVQSIVKGNTPEDRIRQRCVIAT
                     GDTAFAEIMRFDNDPVNAGINAIRNGRSIFTDIRMAQVGITRRGHKCDVRCVLDEGDD
                     IARKTGVTRTSAGFMALETELDGAIIVIGNAPSAALTVCRMIDEGLRPALLVATPVGF
                     VNAAESKEKVRTLLVPSITCVGTRGGTPVAVAVVNELVEIQFKNAKIGAK"
     gene            complement(1634..2401)
                     /gene="cobJ"
                     /locus_tag="VKL21_06375"
     CDS             complement(1634..2401)
                     /gene="cobJ"
                     /locus_tag="VKL21_06375"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HMB45429.1"
                     /translation="MEMSQSRSRGKLYIVGIGPGAIEHLTKKAESVLLESEYIIGNGT
                     YLDQIAEVIKNKKIIRSGMGGEVQRAKKAVELGFDHIVSIISGGDANVYGMAGLVLEV
                     AEKSGDIDLEVIPGVTAISAAASLLGAPLVNDFAVISLSDLLTPVGMIERRLESAAKA
                     DFVIAIYNPKSRNRRQNFSGAVEIIRKYRSENTPVGIVKNATRNGETVIATTLGRIME
                     YNDIIDMSTIILIGNSESRSWKNRIITPRGYQRKYEY"
     gene            complement(2370..>3028)
                     /locus_tag="VKL21_06380"
     CDS             complement(2370..>3028)
                     /locus_tag="VKL21_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HMB45430.1"
                     /translation="VVVDSGLNFAIPILGGHHGGNELAKKLALIDIFPVITTAMEVME
                     KESVEGIASRLGCRIINRESTKKLNMVLLDSDVEVLEIKGPKIVIVADDVSVLKKNGL
                     VVGIGANRGVSKSEVVEAINAAISESNVRIEDVNYFASAMIKENESGIIDAAAVFGKE
                     LRFVPHEIINSMHAPTPSKAKSLGLNGVCEPAALALSEEKRLLLKKRIYGNVTIAIAR
                     "
BASE COUNT          740 a          791 c          572 g          925 t
ORIGIN      
        1 ctgatcctgg aggaattcat cgacacctga tcccaaatca tcaagtaacg ccctggctgc
       61 aatgtcgcct actttttctg cttccagtcc tggttttccc agagctgttc cgccaattgt
      121 accgcaccac agggtaattc cgctgccggt tgaaggataa tcattaacct cagtcgagat
      181 tcttgaagtg taaccatgtt caagcagaag cttttgggct gatgcagcct gtctttgtga
      241 aacatgggaa ggtagaccgg atgaatggga tattccttct acctgagagc gcttgttgga
      301 atcaaaattt attttcttga ggttcgatgg attgataata gcttctacac agcctccacc
      361 cctgggataa tagcctctcc tgataagcct gagttcgcat tcatatccca tcatcctcaa
      421 ggcgaaaagt gttacaaacc tcatataatc aatagaagga gaccaccgga catcagttcc
      481 acctgttatt tttatcctga cggttcccgg cgcattcata agtgcgggca taaggcactg
      541 gagaaaaaga gaaatacttc ccgcagtccc gatatctatc tcataatttc cgccatgtat
      601 ctttccgggc gtaaacttaa gtcgtgtgga tccgaggtga catccggaaa ccgaagcttc
      661 gcatatcctg gccagccctt ccaccgcctt cacatgctgt gctgatagtc ccggttttgg
      721 cctgcctttt ctaatatttt ctatttccac aggttttccg gaaaccgcag aaagagccac
      781 acaggttcgt aatatctggc cgcccccttc tccgtaagac ccgtcaatct tcataaatta
      841 ctattgtttg cgataatatt gaagtttgct gaatataata taaaaagaat gacgagcagg
      901 ttggggagtg ggggttagta aaaaaatgtt cccgctcgtc aacttcatct atgtttaaga
      961 tatatttaaa tcttatccag aattttgacc cttacaatta ttatcatgca tttatttggc
     1021 tcctattttt gcgttcttga attggatttc cacaagttca ttgacaactg cgaccgccac
     1081 tggagttccg cctcttgtcc caacgcatgt gatagatggc actaaaagtg ttcggacttt
     1141 ttctttcgat tcggccgcat tcacaaaacc cacaggtgtc gctacaagaa gcgccgggcg
     1201 aagaccttca tctatcatcc tgcaaacagt gagcgcagct gaaggtgcat ttccaataac
     1261 gattattgcg ccatccagtt ccgtctcaag agccatgaag cccgcacttg ttcttgtcac
     1321 tcctgttttc cgggcaatat cgtctccttc atcaagaacg catctgacat cacatttatg
     1381 tccccgcctg gtgataccca cctgcgccat cctgatatcc gtaaagatag atcgcccatt
     1441 cctgatagca tttattcctg catttaccgg gtcattgtca aacctcatga tttctgcaaa
     1501 tgcagtgtca cctgttgcaa taacgcagcg ctgtctgatc ctgtcttcag gcgtatttcc
     1561 tttcacaata ctttgtacta ttgaccagct cttttcattg atctcttttg cttcccgtgt
     1621 gcgtgcgccg aactcaatat tcatatttcc tctggtatcc tcttggtgtt attatcctgt
     1681 ttttccatga tcttgactcg ctgttaccga taagaataat cgtactcata tcgataatat
     1741 cattgtattc cattatcctg ccaagggttg tcgcaattac cgtctcacca ttgcgagtgg
     1801 cattttttac gatcccgaca ggtgtatttt cactcctgta ttttctgatg atctcaaccg
     1861 cccccgaaaa attctgcctg cgattcctgc ttttcggatt ataaatggcg atcacaaaat
     1921 cggcttttgc agcgctttcc aagcggcgtt cgatcatccc gacgggcgtg agaaggtcgc
     1981 taaggctaat tactgcaaaa tcattaacaa gcggagcacc gagaagcgaa gctgcagcgc
     2041 ttattgcagt tactcccggg atgacttcca gatcgatatc tccggatttt tcggccactt
     2101 ccaacacaag accggccatc ccatacacat ttgcatcccc gccgctgata atggacacaa
     2161 tatggtcaaa tcccagttca accgccttct ttgcgcgctg aacctcaccg cccatcccgc
     2221 ttcgtattat ctttttgttt tttattacct ctgctatctg gtcaagataa gtgccattcc
     2281 cgataatgta ttcggactca agcaggacac tttcagcctt tttcgtaaga tgctctattg
     2341 ctcccggccc tatgcctacg atgtataatt taccgcgcga tcgcgattgt gacatttcca
     2401 taaatcctct ttttaagtaa tagtcttttt tcctcagaaa gtgcaagtgc agccggttcg
     2461 catacaccat tcaacccaag gctttttgct tttgatgggg tgggtgcatg catggaattt
     2521 atgatctcat gcggaacaaa tctcagttct tttccaaaca cagctgctgc atcaataatt
     2581 cctgattcat tctccttaat cattgcagat gcaaaataat tcacatcctc gatcctcaca
     2641 tttgattccg aaatcgctgc atttattgct tccacaactt ctgacttgct tacaccccga
     2701 tttgcgccta ttcccacaac cagtccgttt tttttaagga cactgacatc atctgcgaca
     2761 attacgatct ttggaccttt tatttccaga acttcaacat cgctatccag caataccatg
     2821 ttgagctttt tggttgattc cctgtttatt atcctgcatc caagacgaga tgcgatccct
     2881 tccacagatt ctttttccat aacttccatt gctgtcgtta tcacaggaaa gatatcaatg
     2941 agggcaagtt tttttgccag ttcattcccc ccatggtggc ctcccaggat aggaattgca
     3001 aaattcaagc ctgaatcaac gaccacaa
//
LOCUS       DATGBN010000263         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_253984, whole genome shotgun sequence.
ACCESSION   DATGBN010000263 DATGBN010000000
VERSION     DATGBN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253984"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1455)
                     /locus_tag="VKL21_06385"
     CDS             complement(118..1455)
                     /locus_tag="VKL21_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HMB45431.1"
                     /translation="MKKTRKSNSKKPSFESDVIEKDLCTLFTKEWTKNAAKETGLIKR
                     ERKIEAFVMLWTLVFSFGAHLPRNLANMKRKYEKASKKEIAESSWYMRFTPELVEFLK
                     VCVTHAIEQLALEQNKVLSEKLAKFKDVLIQDSTIIRLHKSLAKKWPAARSKTVAAGV
                     KVGMLVSAVANSPKSIGIYAETVNELKTLRIGPWIKDRILLIDLGFYKHQLFAKIKEN
                     EGYLVSRLKGNADPLIIDLYNTCPGNSIDVKGKYLSEIISKLKRQVLDVEVEISFKKP
                     NYNGKSKTKMDTERFRLVAVYNIDEEKYHVYLTNISNDDLSPEDVSKLYGARWDIELV
                     FKELKSRYDLDVVNTKNPQIVEAYIWIAILTLFISRRIYNIVRKHNPKEKLIRYTQLR
                     WSTIFTENASDQLTLILRYYGVERTFETVMNVYSSQALDPHVNRERFRDEFWS"
     gene            2087..>2527
                     /locus_tag="VKL21_06390"
     CDS             2087..>2527
                     /locus_tag="VKL21_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HMB45432.1"
                     /translation="MNKNVYTVLEDKTTNNIKIHKSSKTYGSTSIGCDTIILENVILG
                     YPEHTILMEIIKQNKDIEYCDFPGCIIGSNSIIRPNSTIFSNVKTGDHFKTGHNVLIR
                     EKTTIGNNVLIGTNVIIDGNVIIGNNVSIQGNVYIPTNVAIEDNV"
BASE COUNT          786 a          502 c          430 g          809 t
ORIGIN      
        1 agaggaatcc atggagatat cagaacgaaa aatgcactat atttacgaaa atccttatct
       61 gccttttctt gcttttcctt atttttacga ggctagataa gtttcattgg tcatcgttta
      121 agaccaaaat tcatccctaa atcgctctct gttgacatga ggatcaagtg cctgactcga
      181 ataaacattc atgactgtct caaatgttct ctcaactcca tagtatcgaa gtataagagt
      241 cagctgatcg cttgcatttt cagtgaaaat agtactccat cttagctgag tgtaccgaat
      301 caacttctct tttggattat gctttcgaac aatattatat attcgtcgac ttataaaaag
      361 tgttaaaatt gcaatccaga tataagcttc gacaatctgt ggatttttgg tattaacaac
      421 atcgagatcg tacctgcttt tcagttcttt aaataccaat tcaatatccc atctcgcccc
      481 atagagtttg gatacatctt caggacttaa atcatcattt gaaatattag tgagataaac
      541 atgatacttt tcttcatcta tattgtaaac cgctaccaat cggaaccttt cagtatccat
      601 tttcgtctta ctttttccat tataattcgg tttcttgaaa gaaatctcaa cttcaacatc
      661 aagcacttgt ctctttaact ttgatataat ttcactgaga tatttgcctt ttacatcaat
      721 gctgttccct ggacatgtgt tatacaaatc aatgatgagg ggatcggcat tccctttcaa
      781 cctggataca aggtatccct cattttcttt gatctttgca aatagctgat gtttataaaa
      841 accaagatca ataaggagaa ttctatcttt tatccatgga cctatccgca atgtcttcaa
      901 ttcatttaca gtctcagcat aaatcccgat agatttgggg ctatttgcga ctgcacttac
      961 gagcatacca actttgacgc ctgctgctac tgttttggaa cgagctgctg gccatttctt
     1021 agcaagggac ttgtgcaacc tgattattgt actgtcctgt atcaacacat ccttgaattt
     1081 agcaagtttt tcactgagaa ctttattctg ttcaagtgcg agttgttcaa tggcatgagt
     1141 gacacatact tttaggaatt ccacaagttc aggcgtaaat cgcatatacc agctactttc
     1201 tgctatttcc ttcttggatg ctttctcgta tttccgcttc atatttgcaa gatttcgagg
     1261 aagatgagca ccgaaactaa agacaagagt ccagagcatt acgaaggctt ctattttcct
     1321 ctcccttttt attaagccag tttcctttgc tgcatttttt gtccattctt tggtgaataa
     1381 tgtgcaaaga tctttttcga ttacatctga ttcaaaagat ggtttttttg aattcgattt
     1441 tcttgtcttt ttcatgctct taatcctctt tctaatttta acactataaa attagaattt
     1501 ttaagtaata tacgcatgca taggacttaa aggcttcgat acataacgat tatttccatt
     1561 ggagaatgaa atttttgata aaaaatgaag atttataaaa attagaagtt attcataaaa
     1621 cgatattggt agaaacgatt attgttctta accgatgaca gatgtagcat atacatacac
     1681 cgttccggcg cacgatagga aaccggggca atcttcacgc agaacggaac gggtgcattc
     1741 aaaaatcgaa cacagatggc acggatatgg cggatgcaca cggattttta tcctacctca
     1801 tggcgcctgt ccgaactcca tgcaacctca caatcacaaa tatgcatcca caacttttaa
     1861 atcaatgaca gccctcttaa ataaaaaata agtaaagata tcataatcct taaggaacca
     1921 tgatgcagaa ccaccccgag aaccaggagc aaccacattt gaacacagtc cgaaggatcg
     1981 ccaaacaacc tcattttatt catcgcccag atcatcagca tggggctagg ttttttcatt
     2041 cgaaaagagg ctggcgcagg gttcggtata aggggggcgg gaattattga ataagaatgt
     2101 ttacacagtc ctggaagata agactacaaa caacattaaa atccataagt catctaaaac
     2161 atatggttct acgtctattg gttgcgatac aataatctta gaaaacgtaa ttttaggtta
     2221 tccagaacat acaatcctga tggaaatcat taaacaaaat aaggatatcg aatactgtga
     2281 tttccctggc tgcatcattg gttccaattc aatcattcgg cccaattcga caatattcag
     2341 taacgtgaaa accggtgatc atttcaagac aggtcataat gttttgatac gcgaaaaaac
     2401 aacaattggt aacaatgttc ttatcggaac taatgtaata atcgacggga atgttataat
     2461 agggaataat gttagcattc agggtaatgt ttatattcca acaaatgtag cgatcgaaga
     2521 taatgtg
//
LOCUS       DATGBN010000264         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_98815, whole genome shotgun sequence.
ACCESSION   DATGBN010000264 DATGBN010000000
VERSION     DATGBN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98815"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VKL21_06395"
     CDS             <1..533
                     /locus_tag="VKL21_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45433.1"
                     /translation="NKESLLESKVYEVLTEMPRFDLLVMGRPEGSGCYCFANNLLRGI
                     MDRILKNYDITIIDTAAGLEHLSRRIIRDVDELLVVTDGSRRGLQTAERIRELAKSLN
                     LNVKQMHVIANKVTPQNQVQIEEYARELKMDLVGIVPFDEILAGFDLEGRPLAELPED
                     SRALIATGEIARKMGL"
     gene            630..1088
                     /locus_tag="VKL21_06400"
     CDS             630..1088
                     /locus_tag="VKL21_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45434.1"
                     /translation="MILNFQLTNVESRVYSTSEDIRKYKNININYDVNLKNPSLVAQN
                     TPFGEKSVLRVEYTFAINYLSPNIGHIRFEGVTDYYSDEDLKSLKEKWDSGNAPGDIQ
                     NELANTMVANLAPLALLLSKSLGLPPSMPIPVINFQQAQKKKEEPTFYHG"
     gene            complement(1091..1405)
                     /locus_tag="VKL21_06405"
     CDS             complement(1091..1405)
                     /locus_tag="VKL21_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HMB45435.1"
                     /translation="MVSLTGKENDALKTRLTKYLMRDETGIRKSLLKLFLDEKPYTTQ
                     NVFDLLKIQGFDVNYRGISAMVGLMNTRLGILRIDVTGDHNVYSLKDEYKNSVKDTME
                     NY"
     gene            complement(1450..2475)
                     /locus_tag="VKL21_06410"
     CDS             complement(1450..2475)
                     /locus_tag="VKL21_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45436.1"
                     /translation="MEKDNSGSFFNFLPEGCRLCYEGAKMVLFVTGLCGKNCFYCPVS
                     NERREKDIIFANERPVKRDQDLIEEAVSMDALGTGITGGEPLLRLERVIRYIKLLKNK
                     FGRMHHIHLYTALAPNRGILESLKRAGLDEIRFHPPQNLLPNIGRTYYNVSIKEAKKL
                     GICAGIEIPAISHDLNGVISLLKEVDGFLNLNELEFSETNFAELKKQGFVPVNDTSCA
                     ALGSKEVAVSLDYGKLHFCSSRFKDAVQLRERFKRMAKKSARYFDEITEDGTLVYGVI
                     EGDCVDLLKEAGTQEEMFAYKDGIVDTAWWIARDLAGELKKIGCKVSVIERYPMKNGI
                     IVEMTPF"
     gene            complement(2479..3393)
                     /locus_tag="VKL21_06415"
     CDS             complement(2479..3393)
                     /locus_tag="VKL21_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMB45437.1"
                     /translation="MDIEELLAQRASLVDGEMPNLLMDIKPPELAAAVSYAILSKGKR
                     IRPALVTLASEAVGGTADKAYFAAASIELIHSSSLVLDDVIDKSEKRRGRSTVHTIWG
                     TDMALLTVQILAALSLRIAARDPRLIHAIADSLFYMGEGEAMELVEFAKDKKSYLDLA
                     FRKTGSLFSSAAEVGALVGGGDKTQINNLKEFGNHIGLAFQIRDDILDCISREDDLGK
                     PVKKDLFMGRPSIVLLDAMNSGISIERMMKCNNGELMHLVSDSIENAKNVAIEEFDLA
                     KSHIENLDDSYAKTKLNELGEYIITRSK"
     gene            complement(3529..4680)
                     /locus_tag="VKL21_06420"
     CDS             complement(3529..4680)
                     /locus_tag="VKL21_06420"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HMB45438.1"
                     /translation="MYADRINSLPPYLFSAIDKAKARIIKKGVDVIDLGIGDPDMPTP
                     PHIVEAMKISLDNPDRHKYPSYEGMLSFRKAAADWYRRTMNVNLDPDDEVLTLIGSKE
                     GIAHIPLAFLNPGDVSLVPDPGYPVYNIGSILASARPFKMPLLTHNEFLPDLDAIPKD
                     IARKAKLMFLNYPNNPTSATATIKFFEEVVDYASENDIIVVHDNAYSEMTYDGYKAPS
                     FLKVRGAKEVGIEMHSLSKTYNMTGWRLGFAAGNSEILEGLGKVKTNVDSGAFEAVQE
                     AGIAALSGSQDCVRDMNKIYMERRDALLIGLMELGLEVKPPKATFYVWAHVNGPASDF
                     SKMLLEKAGIVATPGIGFGEYGEGYVRFALTQSVERINEAVERMRKLDI"
     gene            4758..>5845
                     /locus_tag="VKL21_06425"
     CDS             4758..>5845
                     /locus_tag="VKL21_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HMB45439.1"
                     /translation="MYEGNSFVDPSVNINVNDFSIGSESYVGPFTSFTGEYTHMGSYS
                     DFQDGVSNSGKITIKDDAVIAHGAHLEGEVEIGSFAFIGFNSIIKDSRIGDGAYIGIG
                     GKINGVDIPPGKSVPQASMIDSIDDIEKLGPVTKEQEEFVEEVIGVNRVLAIGYTRLF
                     EKNGASDFGSIGPNGDGDILIDGKDILARSGNNVPGIGAFSVIGSTRIIGDVFLGENS
                     IVENGTSIRADEGIPIKIGKNAQIGMNNTFHSLNDQEISIGDDLKVGSDSVLHGPLVL
                     GEKTNIGSRAVVFKSTIGNNVEIGDDAIVVGVKIPDGTVIPSGSVVETKKDVRLLNPG
                     AVAPGTKWASIMDLFLVSMIPIILGLVTS"
BASE COUNT         1605 a         1301 c         1244 g         1695 t
ORIGIN      
        1 ccaacaagga gagcctgctt gaatcgaaag tttatgaagt cctcacagag atgccgcgtt
       61 tcgaccttct cgtgatgggg cggcctgaag gttcgggctg ctactgcttt gcaaataatc
      121 ttcttcgcgg gataatggac aggatcctca aaaattatga catcacgata atcgatactg
      181 cggcagggct tgaacaccta tcgcgacgga ttatcaggga tgtggacgaa cttcttgtag
      241 tcactgatgg ctcgcgccgc ggccttcaga ctgcagaaag gatacgagag cttgcaaagt
      301 cacttaacct taacgttaaa caaatgcatg tgatcgcgaa taaggttacc ccgcaaaacc
      361 aggtgcagat agaggaatat gcccgtgaat tgaagatgga ccttgttggt attgtgccat
      421 ttgatgagat tcttgccgga tttgaccttg aaggaagacc gcttgctgaa ttgccggagg
      481 attccagagc gctaatagca acaggtgaga ttgcacggaa gatggggtta tagaagaaca
      541 atttgaaaag taaatggtag atggaaaatt ttccatgtcc cataggaata atttaataac
      601 tcatgaaagt ataataacca tgagaaaaca tgattttaaa tttccagctt acaaatgtgg
      661 aatccagggt gtattctact tcagaggata ttcgaaaata taagaatatc aatataaatt
      721 atgatgtaaa tcttaagaat ccatcattgg ttgcccaaaa cactcctttt ggagaaaaat
      781 cagtattaag ggttgagtac acttttgcca tcaattatct cagtcccaat atcggtcaca
      841 tcaggtttga aggcgtgacg gattattaca gcgacgaaga tctcaagtcc ctgaaagaaa
      901 aatgggactc aggaaatgcg cccggtgata tccagaatga acttgcaaac acaatggtgg
      961 caaatcttgc tcctcttgca ctactgctct caaagtccct gggcctgcca ccttcgatgc
     1021 ctattcccgt cattaatttc cagcaggcac agaagaaaaa agaagaaccc actttttacc
     1081 atgggtgaac ctaataattt tccatggtat ccttcacact attcttatac tcatctttga
     1141 gcgaatagac attatggtcg cctgtcacat ctattctcag gataccaagc cttgtattca
     1201 taagaccgac catggccgaa attcccctgt aatttacatc aaacccctgt atcttcagga
     1261 ggtcaaatac attctgtgtg gtatatggtt tctcatcgag aaacaatttc aatagtgact
     1321 tcctgatacc ggtttcatct ctcatcaggt attttgtaag tcgtgtcttt aatgcatcat
     1381 tctcttttcc ggtcagtgaa accaccaaac cccaatattc gatttttaga gtttatacct
     1441 ttccatggat taaaacggtg tcatttccac tattatcccg tttttcatgg gatatctctc
     1501 tatgactgac accttgcatc ctattttttt caattcgcct gcaaggtccc gggctatcca
     1561 ccaggctgta tcgacaatac catctttata tgcaaacatt tcttcctgtg ttcctgcctc
     1621 tttcaagaga tccacgcagt ctccttcaat aacaccatag acaagtgtcc catcttccgt
     1681 gatctcgtca aaatacctgg cggatttttt ggccattcgc ttaaatcttt cccgaagctg
     1741 cactgcatcc ttgaatcttg aagaacaaaa atgcagtttc ccataatcca gagaaactgc
     1801 tacttctttg cttccaagag ccgcacagga ggtatcattg acaggcacaa atccctgttt
     1861 cttaagttcg gcaaaatttg tttccgaaaa ttcaagttca ttcaggttca aaaagccatc
     1921 gacttccttc aataatgata ttactccatt aagatcatgg gaaattgccg gtatctcaat
     1981 tcctgcgcaa atacccagtt tcttcgcttc cttgatcgaa acgttataat atgtccggcc
     2041 gatatttgga agcaggtttt ggggaggatg gaaccttatt tcatcaaggc ccgccctctt
     2101 taaggattca agtatccccc tgttcggggc aagagctgta tataagtgaa tatgatgcat
     2161 tctcccaaat ttatttttca ggagctttat gtaacgaatc acccgctcaa gtcttaaaag
     2221 cggttcaccc cccgtaatac ctgtaccaag agcatccatc gaaacggctt cttctatcag
     2281 gtcctggtcc ctcttcacag gtctttcatt tgcaaatatt atatccttct ctcgcctttc
     2341 atttgaaacc ggacagtaaa agcaattctt cccgcagagt cctgtaacga aaagaaccat
     2401 tttggcccct tcataacaca agcggcatcc ttctggaagg aaattgaaaa aggaaccgct
     2461 gttatctttt tccatgtgtt atttacttcg ggttattata tattcaccca attcattaag
     2521 ctttgttttt gcatagctgt catcaagatt ctcgatgtgg ctttttgcaa gatcgaactc
     2581 ttcaatcgcc acatttttcg cattttctat agaatcagaa acaagatgca tgagttctcc
     2641 attattgcat ttcatcattc tttctattga gatcccggaa ttcatggcat cgagaagtac
     2701 aatggatggc cgtcccataa aaagatcttt ctttacgggc ttacccaggt catcttctct
     2761 tgaaatgcaa tccagtatgt catcgcgtat ctgaaaagca agacctatgt gatttccgaa
     2821 ttccttaaga ttatttatct gggtcttatc ccccccgcca acaagagcgc ccacttcggc
     2881 agccgaactg aaaagtgaac ctgttttcct gaaagcaagg tcaagatagc tttttttatc
     2941 ttttgcgaat tctacaagtt ccattgcttc accttcaccc atatagaaca gtgaatccgc
     3001 aatggcatgg atgagtctgg ggtcacgggc tgcgatcctg agtgagagcg ctgcgaggat
     3061 ctgtacagtc agtaaagcca tatcagtgcc ccatatggta tgtaccgtgg atctgccgcg
     3121 ccttttttcc gatttgtcga tcacatcatc cagcacgagc gacgaggaat gtatcaattc
     3181 aatagacgcc gctgcaaagt aggctttatc agctgtcccg ccaacggcct cactggcaag
     3241 cgtcacaagg gccggcctga tgcgttttcc ttttgataat atggcataac tgacagctgc
     3301 ggcgagttcc ggaggtttga tatccataag caggtttggc atttccccgt ccaccaaact
     3361 tgcacgctgt gccaaaagtt cctctatgtc cattattcac accataacct gaatacttga
     3421 aaaggatttc tatttaatag catatttatt cacaatttac actaatcata aatagatttt
     3481 atcttttttt tctatgaatc ttaattgatc tataactata attcttcatc atatatccaa
     3541 cttgcgcatt ctttcaacgg cttcgttgat cctttccacc gactgagtaa gagcgaatcg
     3601 tacataccct tcaccatatt ctccgaatcc gatgccagga gtggccacaa ttcctgcttt
     3661 ttcaagaagc atctttgaaa agtccgaagc tgggccgttc acatgcgccc atacatagaa
     3721 agtagctttg ggcggtttta cttcaagccc cagttccata aggccgataa gaagagcatc
     3781 gcgcctttcc atatatattt tattcatatc acgaacacaa tcctgggacc cggaaagcgc
     3841 tgcgattccc gcttcctgaa cggcttcaaa tgcgccggag tccacattcg tttttacttt
     3901 tccaagacct tccagtatct cactgttacc ggctgcaaaa ccaagtctcc agccggtcat
     3961 gttatatgtt tttgagaggg aatgcatctc gatgcctacc tcttttgccc ccctgacttt
     4021 caggaaactg ggcgccttgt agccatcgta ggtcatttcc gaataggcgt tatcatgtac
     4081 aacgatgata tcattttcac tggcataatc cacaacttcc tcaaagaatt taatggttgc
     4141 agttgctgat gtgggattat tgggataatt taagaacatg agctttgcct tcctggcgat
     4201 atcctttggt attgcatcca ggtcgggaag gaattcgtta tgggtgagga gcggcatctt
     4261 aaaaggcctg gcgcttgcaa ggattgaccc aatattatag acaggatacc ccggatcggg
     4321 aaccaaagac acatcccccg gattcaggaa cgcaagcggt atgtgggcaa tcccttcctt
     4381 tgaaccaata agggtaagaa cttcgtcatc gggatccagg ttaacattca ttgttctcct
     4441 gtaccagtca gctgctgcct tcctgaaaga cagcatgcct tcataagatg gatatttgtg
     4501 cctgtccggg ttatccaggg atattttcat cgcttccaca atatggggcg gggtaggcat
     4561 atccgggtcg cctattccca ggtcaataac atcaacgccc ttcttaatga tccgggcttt
     4621 ggctttatct atagcagaaa ataaataagg cggcaatgaa ttgatcctgt cagcgtacac
     4681 tatcataggg cgttttgatg ttttttcttg tatataagat atgagttttg attgttgggg
     4741 catccaacaa tacagttttg tatgagggga atagtttcgt tgatccttcg gtcaacataa
     4801 atgtgaatga tttctctatt ggttctgaaa gttatgttgg tccgttcact tcgttcacag
     4861 gtgaatatac ccatatggga agttacagcg atttccagga cggtgtcagt aattccggga
     4921 agataactat aaaagatgat gctgtgatcg ctcatggagc ccatcttgaa ggagaagtgg
     4981 agatcggaag tttcgcattc attggattta actcgattat caaggattca agaatcggtg
     5041 atggcgctta tataggaatt ggtggcaaga tcaatggcgt tgatataccc cccggcaaat
     5101 cggtgccgca ggcatcaatg atcgacagca tagatgacat tgaaaagctt ggaccggtta
     5161 caaaagaaca ggaagagttc gtggaagagg tcataggcgt caatagagtc cttgcaatag
     5221 gatacacccg gttgtttgag aaaaatgggg catccgattt tggtagtatc ggtcctaacg
     5281 gagatgggga tatattaata gatggcaagg atattctggc acgaagcgga aataatgtac
     5341 ctggtatcgg agcattttct gttattggat caacccgaat aatcggggat gttttcctgg
     5401 gtgagaatag cattgtagag aatggaacat ccataagggc ggatgagggg atcccgataa
     5461 aaatcggaaa aaatgcacaa attggcatga acaatacatt ccattccctg aatgatcaag
     5521 agatcagtat tggggatgat ctcaaagtgg gaagtgattc ggtattgcat ggaccgctgg
     5581 ttcttggtga gaagacaaat ataggtagca gggccgtggt ctttaaatcc acgatcggaa
     5641 ataacgtgga aatcggtgac gatgccatag ttgtaggtgt gaaaatccct gatggaaccg
     5701 tgataccatc gggttctgtt gtcgagacga agaaagatgt gagactgttg aatcccggcg
     5761 ctgttgctcc aggtacaaaa tgggcgtcaa ttatggacct attccttgta tctatgatcc
     5821 cgatcattct tggccttgtg acgtc
//
LOCUS       DATGBN010000265        10530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_98864, whole genome shotgun sequence.
ACCESSION   DATGBN010000265 DATGBN010000000
VERSION     DATGBN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10530
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98864"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VKL21_06430"
     CDS             complement(<1..440)
                     /locus_tag="VKL21_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMB45440.1"
                     /translation="MELEKIEKILEKEPNEAAEELLKEVEKAYGEVPYILNFMRRSPE
                     LLVTKILYDNAIIREFKRLDAKTIELISIGVSAALRCDHCLKMHIRVAQRIGVTKEEI
                     FDAILIAGTLSNASVLAEGTRAMDSENLNVSDKEKCKVCGIPKE"
     gene            complement(531..1295)
                     /locus_tag="VKL21_06435"
     CDS             complement(531..1295)
                     /locus_tag="VKL21_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase"
                     /protein_id="HMB45441.1"
                     /translation="MPRDRRDFFYKKAKEEGFRSRAAFKLKQINEKFKMIEKGDTVVD
                     LGAAPGGWLQVAKELSGGKVVGVDILAIEEIIGVDTIKGDIRLDRTIEKIRGMIKKDG
                     ADVVLCDAAPNLSGSWSYDHARSIDLATCAFECARKLLKNGGSFAVKVFQGDMFPDYL
                     NKVRECFGKVQAFSPVASRKQSAEIYIIGKELLTDAVKINGEYAVKIEDIGANGDGIA
                     KINDFVVFVKYARKGEELNIRIRFIKPNFAFGDRID"
     gene            complement(1297..2226)
                     /locus_tag="VKL21_06440"
     CDS             complement(1297..2226)
                     /locus_tag="VKL21_06440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB45442.1"
                     /translation="MLIHKILGLSDNENICFVEESYASLNNNILTLQYLENVRKDPDA
                     RISGIKDENGNIIGLVLYNVESEESESFLAIFSNIISSGPNPNNSSRIFSEIAKYLKL
                     EHLYNTTSQKFIEAVIEYFSIELVNRHLCENCHIKKEPYNMVYIESRNARLNGILEKY
                     KLKGKILEICCGNGMSTLPLINNGYNPLSVDYDKCQICQGLEHSALDPKRTIVIDATK
                     LSEFFKENTFDTIVGFMLGTIYPFNKGIWERMMAEAVKILKPEGMILLTVNKKEEIEI
                     LKNALEIKGIHGELIDNTDSKGIYDQWIYIGNK"
     gene            complement(2234..2833)
                     /locus_tag="VKL21_06445"
     CDS             complement(2234..2833)
                     /locus_tag="VKL21_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HMB45443.1"
                     /translation="MQVQTKIIPFYTEERWQNWIIQVKESGFKIDDQQKGAIFVNMED
                     DVVLACLKIIAKLDKNSFSKEEALGQISEIKEIVLKQIEPITEDIDMMIESTQLSLMG
                     VFASCECYIEKAFEKTKSLKPLIKKAIEAEKEENMGAVLGNIAEIGANILAGGKIKDK
                     DLEDIPDGLVAEWLDGIDSIRAAMIGDTSYRDDEPDEGK"
     gene            complement(2863..3276)
                     /locus_tag="VKL21_06450"
     CDS             complement(2863..3276)
                     /locus_tag="VKL21_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45444.1"
                     /translation="MMCPDKSIKKVLVVDDEPDTLELVKLVLESAGFEAILANNGMEA
                     LAQIADVDPDLVLLDIMMPDMDGWEVFRKIKEKDSSIPIAILTAKAQNIDKLLGLHVL
                     KADDYITKPFGKNELIGKVKKLTGSTDKKQITTIN"
     gene            complement(3288..5936)
                     /locus_tag="VKL21_06455"
     CDS             complement(3288..5936)
                     /locus_tag="VKL21_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45445.1"
                     /translation="MSSLRGRIIVSMLAIILLILSGSYFVIQDIEAGIIEGEFRNEGF
                     LLANNLASEITNNFLLNDIVEVRKSLDNAKKSYPEIEYIFVTDSQGIVLTHTFENGFP
                     KALLNFTKPENASKEEILETGNGIIHEFDAVLFKNIGYVHIGLSENKVRAKILDASRK
                     ILFLAISAIILGAIFIYFIGKRLTEPILKLNEGAKRINNGILDQKIEVSSNDEMGELA
                     KTFNDMSSNLDQKIKELLASKEKTEDAENYLETLFNNIEDGIIVINTNHEIIKFNSSF
                     LKMMGLSEKEVMGKTCHELIFRSLPAQQQKEKCQVNILLKSRKPIRFLHEAQFDDKTK
                     ILDINSSLFLDKKGNANIIMVIRDVTHHNVLENEIIVRNHELTVLNEISKNISEAFDI
                     DILLSGSLENILKLTNMECGGAYLIDDKTGQFTMINHQGPAGCSDTFELKKTNEVLIV
                     EGVKNNDPSMDNYISFAIIPLKSKDHVLGIISINSKESHIFSERDKELFSAIGNQIGV
                     AIENINFYNNIKHLKEFNDEILNNVNLALHVVDRDLRILSVNDELIKLGKGMIKRDDL
                     LNKNLLDVYPFLTEKRIDLEYDHVIKTGEIFQSEEKTQYYDEIIYTSTSKIPIKDKNG
                     YVEKIITIIKDVSNQKKLEEELRDSYAELKLTYSKLQELYKIKDNFLSNISHELRTPL
                     TSVIGYTELMLDENLTDQQRHKTEIIFRNSKRLFRLIRALLDTQMIESSNLQITKETV
                     MINELIYAVVEDMKNISASKNIPISINIQDNLVVDGDNERLMQVFSNIVENAIKFTIT
                     GEIIIKGEIDKGKVHITISDTGIGIPEERLKRIFDRFYQVDSSNKRKFQGAGLGLWIS
                     KKIIEAHGGRIWAESKNRGSTFHILL"
     gene            complement(5933..6844)
                     /gene="phnD"
                     /locus_tag="VKL21_06460"
     CDS             complement(5933..6844)
                     /gene="phnD"
                     /locus_tag="VKL21_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HMB45446.1"
                     /translation="MKYEFLLFCIFILLISGCIDKTEKTDLNAVQEPETIINDSSVKV
                     AIASVVSPKESYEYYEDMIRYISRSLGGNVKIIQRRSYKEINDLIKKNQIDFAFVCSG
                     AYIDGNSEFGMKILVAPVVSGNTTYHSYIIVRNNSNYTGLSDLRGKKFAFVDPLSNSG
                     KLFPTYRLYLMNETPGSFFGIDEQGKNNSFYTYSHDNSIIAVAQKIAEGAAVDSLVYE
                     NMKETHPETIAKTRIIETSPPFGNPPIVVSKDIDPFLEERLRNIFLNMDEDEDGKKVL
                     DKVKIEKFVNLSNNAYDSIREMRKRVQ"
     gene            complement(7149..7430)
                     /locus_tag="VKL21_06465"
     CDS             complement(7149..7430)
                     /locus_tag="VKL21_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45447.1"
                     /translation="MDLDFFLRFFNIYSWAVASIIMIFMAAIARFYQKKFEIQTYYYL
                     YFIPAIVFFIVLLQIFPFFDIEAELIEFFGSAISVMAGYFLYMKMVGVK"
     gene            complement(7438..8880)
                     /locus_tag="VKL21_06470"
     CDS             complement(7438..8880)
                     /locus_tag="VKL21_06470"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB45448.1"
                     /translation="MKQVKSGINGLDKVLEGGFLRPSIVLIAGTAGTGKTTMVMQSLF
                     NSARNEEICMYITALSEPIAMINNFMSKFSFYNISLLGKGNVKYVPIGIDIIHKGSEA
                     IIKEMERNIETIKPDRIVIDPINVFALGEDEESQRQFFYDFFTSMKGWNSLVLLTGEF
                     TQDALINSTLGYMVDGMIYISYEPFYENNIRYLNVLKMRGQEFNGGKHSCKINKDGFN
                     VYPRIRATEMTAISNERISTGIKGLDKMIGGGFIKSSSILISGSSGTGKTILGTQFIM
                     DGLSKNEPGIIVSFEEDVEQIRANSNMFNWNLQEFENKNLLRIISPLEYDAYELALQI
                     EETIDAIKAKRLLLDGTGRLQRMLPQYAQLPEYMGTISRILKNKKITAVYTNETSNLT
                     GATQITGTGISPFLDTVILLRYVEIKSEMRKAISVLKMRGSDHDKEIREIVINKNGAE
                     IKLPFSEYSGLLTGNPIKLATQAFVEAFKK"
     gene            complement(8956..9321)
                     /locus_tag="VKL21_06475"
     CDS             complement(8956..9321)
                     /locus_tag="VKL21_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMB45449.1"
                     /translation="MTTIEMLEKILNDLNNIGGVEASAVASRDGLLICSTISKKQPAE
                     TFVAMSATMMGAAETATTELGKGIPERIIVESKTGRIIGTGAGAKALLLVMTKPDAGL
                     GLVLIEMTKASGKIKNILG"
     gene            complement(9370..>10530)
                     /locus_tag="VKL21_06480"
     CDS             complement(9370..>10530)
                     /locus_tag="VKL21_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMB45450.1"
                     /translation="NSIRNDFLYWYPVDLRTSGNDLISNHLLFFLFHHVAIFPEIHWP
                     HAIAINGFVSLEGQKMSKSKGPILTLKSAVKEYGADITRLYILSTAEHVQDADWRGAD
                     IESTRKQFERFYKMAFDCLTVGQNTPNPELKFIDKWMLSKLQYRIKEAADALNKMEIR
                     RAVQNSFYHLMNDMKWYERRGGIAVKKEVIDVWIRLMAPFTPHTCEEIQERSGKNFIS
                     LDKFPLADLSLIDRGAELAEEITGNTLRDIEEIIRLIRIKPKRVILYTAASWKKKVLN
                     IAIMMKKDNVLDMKTLMNTVMSDPAMRQFAKEIPKFAQKTITDVQGMELEFMNSLIEV
                     DFNETKALQESVDFLSAEIGCNIEIYSADAPIHDPQGKSRQAIPMRPAIFIE"
BASE COUNT         2900 a         2252 c         1684 g         3694 t
ORIGIN      
        1 ttttcttttg gtatcccaca aaccttacat ttttctttat ctgatacgtt caggttctca
       61 gaatccatgg cacgagttcc ctcagcaagg accgatgcat tggacaatgt ccctgctatc
      121 aatatcgcat caaatatctc ttctttggtc acaccaatcc tctgtgcgac tcttatatgc
      181 attttcaggc agtgatcgca ccgcaaagcc gctgaaacac caattgatat tagctctatt
      241 gtttttgcgt ccagccgttt aaattcccgg attattgcat tatcatacag tattttagtg
      301 accagcaact ctggcgatcg cctcataaaa ttgagaatat aaggcacttc accgtatgct
      361 ttctcaacct ctttcaataa ttcttcagct gcttcattcg gttccttttc aaggatcttc
      421 tcgatttttt caagttccat gggggttcta ataaaacgat taaaatccta aactaaataa
      481 acatttgtat tttggcagaa taacacaatt cttcatatca aactatgctt tcaatctatt
      541 ctatccccaa aagcaaagtt aggttttatg aaccttatcc ggatattaag ttcttccccc
      601 tttcttgcat atttcacgaa cacaacgaaa tcattgatct tggcgattcc atccccattt
      661 gcacctatgt cctctatctt tacagcatat tctccgttaa tttttactgc gtcagtcaat
      721 aattccttcc cgataatata tatctcagcg ctttgcttcc gggatgcaac aggtgaaaaa
      781 gcctggacct ttccaaaaca ttcacgcacc ttattgagat aatccgggaa catatctccc
      841 tgaaatacct ttactgcaaa acttcctccg tttttgagaa gctttcttgc gcactcaaaa
      901 gcgcaggtcg caaggtcgat agaacgggca tggtcataac tccagcttcc cgaaagattt
      961 ggcgcagcat cgcaaagaac cacatctgcc ccatctttct tgatcatgcc ccttatcttt
     1021 tcaatagtcc tatcaaggcg tatatcccct tttattgtat ccacaccaat gatctcctca
     1081 atagccagta tatccacacc tacaactttt ccacctgaga gttcttttgc aacctgtagc
     1141 caacctccgg gcgccgcgcc aagatcgact acagtatccc ccttttcgat catcttaaac
     1201 ttttcattta tctgtttaag tttgaatgct gcgcgggagc gaaatccttc ctcttttgct
     1261 tttttgtaaa aaaaatccct tctatcacgc ggcattttat ttatttccta tataaatcca
     1321 ctggtcataa atgcctttac tatccgtatt atcaatcaat tccccatgta tcccctttat
     1381 ctcaagtgca tttttcagta tctcgatctc ttctttttta tttactgtca aaaggatcat
     1441 tccttcaggt ttcaggattt tcactgcttc tgccatcatt ctttcccaga tgcctttatt
     1501 gaagggatat atagtgccaa gcatgaagcc aactattgta tcaaaagtat tctccttgaa
     1561 gaattcggaa agttttgtgg catcaatgac tattgtcctc ttcggatcaa gtgcactatg
     1621 ttcaagtccc tggcatatct ggcatttatc ataatcgact gaaagtggat tatatccatt
     1681 attgataagc ggcagtgtgg acataccgtt accacagcat atttccagta tcttgccctt
     1741 taatttatat ttttcaagaa ttccatttaa cctggcattt ctactttcaa tataaaccat
     1801 attatagggt tcttttttta tgtggcaatt ttcacataaa tgcctgttta caagttcaat
     1861 tgaaaaatat tcgattactg cttcaataaa tttttgggat gtagtattat ataaatgctc
     1921 taacttaaga tatttggcaa tttctgaaaa tatcctgctg ctattatttg gatttggtcc
     1981 ggatgaaatt atgttagaaa agattgccag gaatgattct gactcttcgg attcgacatt
     2041 ataaagaact aaaccgataa tgttaccatt ttcatcttta atccccgata tcctggcatc
     2101 cggatctttc ctgacatttt ccagatactg cagtgtgagt atattgttat ttaaagacgc
     2161 atagctttct tcaacaaaac agatattctc attatcggaa aggcccagta ttttgtggat
     2221 cagcatctat gtttcatttg ccttcatctg gttcatcgtc acgataactt gtgtccccta
     2281 tcatggcagc tctgatcgaa tctatcccgt caagccattc agcgacaagt ccgtcaggaa
     2341 tatcctcaag gtccttatct tttattttcc caccggcaag tatattagct ccgatctcgg
     2401 caatatttcc caatactgcc cccatattct cttctttctc tgcttctatt gcttttttga
     2461 tcaaaggttt aagcgatttt gttttttcaa aggctttctc tatgtaacat tcacatgatg
     2521 caaaaacacc cattagcgat aattgtgtgg attcgatcat catatcaata tcctcagtaa
     2581 tgggctcaat ttgtttcagg actatttcct tgatttccga aatctgtcca agtgcttctt
     2641 cttttgagaa tgaattctta tctaattttg ctattatttt aaggcatgca aggaccacat
     2701 catcttccat gttaacaaaa atcgctcctt tttgctggtc atcgatctta aacccgcttt
     2761 cctttacctg aattatccag ttctgccatc tctcttcggt ataaaaggga attatttttg
     2821 tttgtacttg catgatatct ctctattgtt ttgaataata ttttaattta tggtcgttat
     2881 ttgtttttta tctgttgatc ctgtcaactt ctttactttg ccgatcaatt catttttccc
     2941 gaatggtttt gttatataat catcagcctt taagacatgc agcccaagca atttgtcgat
     3001 attctgcgct tttgcggtca aaatggcgat aggaatactg gaatcttttt cttttatttt
     3061 cctgaacact tcccatccgt ccatatccgg catcatgatg tcaagaagca caagatcagg
     3121 atctacatct gcgatttgag ccagagcttc catcccgtta tttgccagga tggcttcaaa
     3181 acccgcgctc tcaagtacca atttgacaag ttcgagtgta tcgggttcat catccactac
     3241 cagaactttt tttattgatt tatcaggaca catcatttca ccagttatta caataaaata
     3301 tgaaatgtac tccccctatt tttactttcc gcccatattc tgccaccgtg ggcttcgata
     3361 attttctttg atatccataa acccaatcct gcaccctgga attttcgttt gtttgatgaa
     3421 tccacctggt agaacctgtc aaaaatcctt ttaagccttt cttcaggaat accgatcccg
     3481 gtatcactga tcgtaatatg aacttttcct ttgtctattt ctccttttat aattatctca
     3541 cctgtaattg taaatttaat ggcattttct acaatatttg aaaatacctg catcaaacgt
     3601 tcattatccc catcaaccac gagattatcc tgaatattaa tggagatagg aatattctta
     3661 ctggctgaga tgttcttcat atcttccaca acagcatata tcagttcatt tatcattacc
     3721 gtttcttttg tgatttggag attacttgat tcgatcatct gggtatcaag aagcgccctt
     3781 atcaacctga ataaacgttt tgaattcctg aatatgatct cggttttatg acgttgctga
     3841 tcagtcagat tctcatcaag cattaattca gtgtatccga taaccgatgt aagcggagtt
     3901 ctgagttcat gggatatatt tgaaaggaaa ttatccttta ttttatagag ttcctgaagt
     3961 ttagaatatg ttaatttgag ttctgcataa gagtccctga gttcctcttc cagttttttc
     4021 tggttggata catccttaat gatggttata attttttcaa catatccatt cttatccttg
     4081 ataggtattt tgcttgtact tgtatagatt atttcatcat aatattgtgt tttttcctcg
     4141 ctttgaaaaa tctctcctgt cttgataaca tgatcatatt ccagatcgat acgtttttca
     4201 gtaaggaagg gatacacatc aagaagattc ttattcaaaa gatcatctct tttaatcata
     4261 cctttcccaa gttttattaa ttcatcattt acagaaagga ttctgaggtc tctatctaca
     4321 acatgtaatg caaggttcac attgttcaag atttcatcgt taaattcttt aaggtgtttt
     4381 atattattgt aaaaattgat attctctata gctacaccga tctggtttcc tatggcagaa
     4441 aagagttctt tgtctctctc tgaaaaaata tgactttcct tgctgttgat tgatattatc
     4501 cctaaaacat ggtcctttga tttaagagga ataattgcaa aagaaatata attatccatt
     4561 gaggggtcat tatttttcac accttctacg atcaggactt cgtttgtctt ctttagttca
     4621 aaagtatcgc tacatcctgc cggaccctga tgatttatca tggtaaattg gcccgtttta
     4681 tcgtcgatca gatatgctcc cccacattcc atatttgtta atttcagtat attttcaagt
     4741 gatccggaca ataaaatatc aatatcgaat gcttcgctga tattctttga tatttcattg
     4801 agcacggtaa gttcgtggtt tcgaacaata atttcatttt caagaacgtt atggtgtgtt
     4861 acatccctta ttaccattat aatatttgca ttaccctttt tgtcaaggaa cagcgatgaa
     4921 tttatatcca ggatctttgt tttgtcatcg aattgggcct cgtgaagaaa tcgtatcggc
     4981 tttcttgatt tcaataatat attgacctgg cacttttctt tttgttgttg tgcgggtaaa
     5041 gaccggaaaa tcaattcatg gcatgttttt cccatcactt ctttttcgct caatcccatc
     5101 atcttcaaaa aagagctgtt aaatttgata atttcatggt tagtgtttat gacaataata
     5161 ccatcttcaa tattattgaa caatgtttca agataattct cggcatcctc tgttttttct
     5221 tttgacgcca ggagttcttt gatcttctga tccaggttcg aagacatatc attaaatgtc
     5281 ttcgcaagtt cacccatttc gtcatttgaa cttacttcga tcttctggtc aagaatgccg
     5341 ttatttattc tttttgcccc ttcattaagt tttaagatgg gctcagtcaa tcgttttcct
     5401 atgaaataaa taaatattgc gccaagtatt atagccgaaa ttgccaaaaa caggatttta
     5461 cgcgatgcat caagtatttt tgctctcact ttgttctcgg acagcccgat atgaacataa
     5521 ccaatattct taaaaagcac agcatcaaac tcatgtataa ttccatttcc ggtctccaat
     5581 atttcttcct tactggcatt ttcaggtttt gtaaaattta acagcgcttt tgggaacccg
     5641 ttctcaaacg tatgtgtaag cacgattccc tgagaatcag taacaaaaat atattcgatc
     5701 tcagggtagc ttttctttgc attatccaat gatttcctta cttcaacaat gtcatttaga
     5761 agaaaattat ttgttatttc cgaggcaaga ttatttgcca ataaaaatcc ttcattcctg
     5821 aactctccct cgattattcc agcttctatg tcctgaatta caaaatatga acctgacagt
     5881 atcaaaagta tgattgccag catagagaca ataatccgac cgcgcaagct gctcattgta
     5941 ctcgtttcct catttccctg atagaatcat atgcattgtt actgagattt acgaattttt
     6001 caatctttac cttatcaagt actttctttc catcctcatc ttcatccata ttcaggaaaa
     6061 tattcctgag tctttcttcc aggaaagggt cgatatcttt agaaacgaca atcgggggat
     6121 taccaaaagg cggtgaggtt tcaataatcc tcgtttttgc tattgtttca ggatgcgttt
     6181 ccttcatatt ctcatatacc aggctgtcaa ctgccgcgcc ttctgcaatt ttctgggcca
     6241 ctgcgataat ggaattatca tgactatatg tataaaatga attgttcttt ccctgttcat
     6301 cgatcccaaa gaatgatcca ggcgtctcgt tcatgagata aagcctgtat gtcggaaaaa
     6361 gttttcctga atttgacaat gggtctacaa aagcaaattt ttttccgcga agatctgata
     6421 aaccggtata attgctatta ttccggacta tgatgtatga atggtatgtc gtattaccac
     6481 tcactaccgg cgcaacaagg atcttcatcc cgaattctga gtttccatcg atgtatgcac
     6541 cgctacaaac aaaagcaaag tcaatttgat tttttttgat aagatcattt atttccttat
     6601 aacttcttct ctgtattatt ttaacattac cacccagcga tcgcgaaata tatcggatca
     6661 tatcttcata atattcatat gattcttttg gggaaacaac agatgcaatt gccactttaa
     6721 ccgaagaatc gttaattatg gtttcaggct cctgaactgc attaaggtct gttttttctg
     6781 ttttatcgat acatcccgat atcaataaaa taaatataca aaataataga aattcatatt
     6841 tcattacaat tatcctctaa aagattctat tattaaccag ccccagtatc tgattgaggc
     6901 gatgaggcca agagtcattc cgattgaaaa aaaaacatac cccaataatt ttgtttcctg
     6961 caatatatca aaagagagag acataattat tgaaccaaat aaagtaaaaa aaattcccag
     7021 ataataaaga taataatact tccttagtcc catgacctgt cctaatcgtt tgctgatcac
     7081 gcttaagaaa aaaagtatta aagaaaaaaa tagaaaagat acagaatcca ataaccctgc
     7141 atatatgttc attttacacc taccattttc atgtatagaa aatagcctgc cataactgaa
     7201 atcgctgagc cgaagaattc tattaattct gcttcgatat caaaaaaagg aaatatttgt
     7261 agaagtacaa taaaaaatac aattgcaggt ataaaatata aataataata tgtttgtatt
     7321 tcgaatttct tttgataaaa tctggctatt gcagccataa agatcatgat tatactcgct
     7381 actgcccatg aatagatatt aaaaaatctc aggaaaaaat ctaaatccat aattcattta
     7441 ttttttgaac gcttccacaa aagcctgtgt tgcaagctta ataggattgc ctgttaataa
     7501 acctgagtat tcactgaaag gcagcttgat ttcagcccca ttcttgttga taactatttc
     7561 ccttatctcc ttatcatgat cgcttcctct catcttaagt actgatatgg cttttcgcat
     7621 ttcgcttttt atttcaacat accgcagaag tatgaccgtg tctaaaaaag gcgatatgcc
     7681 cgttccggtg atctgcgttg cccctgtcag gtttgaggtc tcattagtat agactgctgt
     7741 tattttttta ttttttagaa ttctgctgat agtccccata tattcaggta attgcgcata
     7801 ttggggcaac atcctctgga gcctgcctgt tccatccagc agtaaacgtt ttgcttttat
     7861 tgcatcaatt gtctcttcga tctggagagc cagttcatac gcatcatatt ccagagggga
     7921 tattattctc aggagatttt tattttcaaa ttcctgcagg ttccagttga acatattcga
     7981 atttgcacgg atctgctcca catcctcttc aaaagaaact attatgccgg gttcattttt
     8041 tgacaggcca tccatgatga attgcgtccc aagtattgtt tttcctgttc cgcttgagcc
     8101 tgagatcaat attgaagagc tctttatgaa gcccccccct atcattttat ccagcccttt
     8161 tattccggtt gatatgcgtt cattggaaat ggcagtcatc tctgttgccc ggatcctggg
     8221 ataaacattg aatccatctt tgtttatctt gcaggaatgc ttgccgccat tgaattcctg
     8281 ccccctcatt tttagaacat tcaggtaccg gatattattt tcataaaatg gttcatagct
     8341 gatatagatc ataccgtcga ccatatatcc aagtgtacta ttgataagtg catcctgggt
     8401 aaattctccg gtcaatagaa caagagaatt ccaacctttc atacttgtaa aaaaatcata
     8461 gaaaaattga cgttgtgatt cctcatcctc accgagagca aatacattta ttgggtcaat
     8521 gacgatcctg tccggtttga tggtctcaat atttctttcc atttctttta tgatggcttc
     8581 ggaacccttg tggatgatat cgatgccgat tggcacatac ttaacatttc cttttccaag
     8641 aagcgaaata ttatagaaag agaattttga catgaaatta tttatcatgg caattggttc
     8701 ggaaagtgcg gtaatgtaca tgcagatttc ctcatttctt gcggaattaa agagcgattg
     8761 cattaccatg gttgtttttc cagtgcctgc agtcccggca atcaatacaa tagagggtct
     8821 taaaaaacct ccttcaagca ctttgtcaag accatttatt cctgatttaa cctgcttcat
     8881 actacctcat tttataattt cagactataa caacctttct atatggttcc tattgaaata
     8941 ccagaaagga agccatcatc ctagaatatt ttttatcttc cctgatgctt ttgtcatctc
     9001 aattaagacc aaaccaagac ctgcgtctgg ttttgtcatg accaggagca atgcttttgc
     9061 ccccgcccct gttcctatta ttctccccgt ctttgattca acgattattc tttccgggat
     9121 cccttttcca agctctgtag ttgctgtttc cgccgccccc atcatagtag ccgacatagc
     9181 aacaaaagtc tcagcgggct gttttttgct tatggtggaa cagatcagca aaccatccct
     9241 gcttgcaacc gctgatgctt ctacaccccc gatattattg agatcgttta agatcttctc
     9301 tagcatttct attgttgtca tattttttta ataaatttta tttattttgt taataattcc
     9361 attttattct tattcaataa atattgctgg tctcatcggg attgcttgcc tgcttttccc
     9421 ctgcggatca tgtattggcg catcagcgct gtaaatttcg atattacatc ctatctcggc
     9481 gcttaagaaa tccactgatt cctggagagc ttttgtttcg ttaaaatcca cttcaatcaa
     9541 ggaattcata aattccaatt ccatcccctg gacatcagtg attgtttttt gcgcaaattt
     9601 cgggatttcc tttgcaaatt gcctcatagc aggatcgctc atgactgtat tcatgagggt
     9661 tttcatatca agaacattgt cttttttcat cattatggca atattcagga cttttttctt
     9721 ccaggacgct gcagtatata gaattaccct ttttggctta attctaatga gccggattat
     9781 ttcttctata tccctgagag tattaccggt gatttcttct gcaagttccg cacccctgtc
     9841 aatgagtgaa agatctgcta gcgggaactt atccagggaa ataaaattct ttcctgatcg
     9901 ttcctggatt tcttcacaag tgtgcggtgt aaatggcgcc attaacctga tccagacatc
     9961 tatgacctct tttttgacag ctattcctcc gcgccgttcg taccatttca tatcgttcat
    10021 caaatgataa aaagaattct ggaccgctct tcttatttcc attttattca gggcgtcagc
    10081 agcttctttt atcctgtatt gtaatttact cagcatccat ttatcaatga acttcaattc
    10141 gggatttggg gtattttgtc caacagtcag gcaatcaaaa gccattttat agaatcgttc
    10201 aaactgtttt cgcgtggatt caatatctgc tccacgccaa tcagcgtcct ggacatgctc
    10261 tgctgtgcta aggatataaa gacgcgttat atccgcgcca tattccttga cagcggattt
    10321 caatgtgaga atgggtcctt tggatttact catcttttgc ccttcaagcg aaacaaaacc
    10381 attgatggca atagcatgcg gccagtggat ttccggaaat atggctacat gatggaataa
    10441 gaagaacaaa aggtggttgg agatcaaatc attccctgaa gtgcgaaggt caacgggata
    10501 ccagtaaagg aaatcgttcc tgatagaatt
//
LOCUS       DATGBN010000266         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_292564, whole genome shotgun sequence.
ACCESSION   DATGBN010000266 DATGBN010000000
VERSION     DATGBN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292564"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VKL21_06485"
     CDS             complement(<1..291)
                     /locus_tag="VKL21_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HMB45451.1"
                     /translation="MLVKQIISEDILTLSEVRNLLEEVKEERSKEDKELGYELRKAIS
                     HAEMFAMLEPQKSREFMDELIKLEKMKPEIAVRIVDILPLTNDEVRSIYAKER"
     gene            complement(303..593)
                     /locus_tag="VKL21_06490"
     CDS             complement(303..593)
                     /locus_tag="VKL21_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HMB45452.1"
                     /translation="MAKTHGTRRKSRYKLKKTVREKGLSPISRAIQEFHEGDIVNIDL
                     DSSIQNGMPHPKFQGRTGKVMSQRGRAYIVEVRDGGLMKEVIILPEHLTPQK"
     gene            complement(622..1860)
                     /locus_tag="VKL21_06495"
     CDS             complement(622..1860)
                     /locus_tag="VKL21_06495"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HMB45453.1"
                     /translation="MIILDTARKIMHEGPICDHCLGRQFAKLSTGLSNDMRGASLKLV
                     LAMHAGAEKDEELKKKLARNSGEMKCWVCNDLFRNIDSWVTKSVQALSDYEYNNFLVG
                     TKVTGLLAENEEILWAESGTTFAEPLKTELNREVGKRIEKLTGKKANLKKPQVVALLD
                     LENDNVEVEINSLYIYGRYRKLVRGIPQTRWPCRECGGKGCQRCDNTGKMYQESVDEL
                     IKPHLLAAARCADTAFHGAGREDIDALMLGDGRPFVVEAKRPHIRSLDLYELEAQIKR
                     GAQGKIEVLGLKFVESETVEQLKSMKVDKEYRLKITHNTTEEKLKSSINVISKTLIKQ
                     KTPTRVLHRRADLERIRKVHSVELESYDENTAVLIINCEGGLYVKELVSGDGGRTIPS
                     VASLTGSEAKVIELEVIKVG"
     gene            complement(1866..2471)
                     /gene="trmY"
                     /locus_tag="VKL21_06500"
     CDS             complement(1866..2471)
                     /gene="trmY"
                     /locus_tag="VKL21_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HMB45454.1"
                     /translation="MKDFIIIGHKAVTETFSLNDLPGVAGRMDILCRCVNAALFLSHD
                     LRRDVRVFLVHKGGSYPPKLVRFEGNAVRYLSPDERSAASLIKKALEKNIQDFWTEST
                     PGVSVRKGDMEGLLKEMDKKIIYLREDGEDIRKKKIDEDFDPLFVLGDHLGLTVEEER
                     IVLGYDPEIMSIGPLSLHADHCIVLLHNEMDRNIFRTPRIM"
BASE COUNT          614 a          652 c          499 g          878 t
ORIGIN      
        1 tcgttctttc gcatagatcg accttacttc atcatttgtc aatggaagta tatctacgat
       61 cctcacagct atctcaggtt tcattttttc aagttttatt aactcatcca tgaattcccg
      121 ggatttctgt ggttccagca tcgcaaacat ttcagcatgg gagattgcct ttcgcaactc
      181 atatccaagt tctttgtctt ctttggatct ctcttctttg acctcctcaa gaaggttcct
      241 tacttcacta agggtcaaaa tatcttcact gattatttgc tttactaaca ttatttcacc
      301 gtctatttct gtggggtcaa atgctcgggc agaatgataa cttccttcat tagtccgcca
      361 tccctgactt caacgatata ggcgcggccg cgctggctca taacttttcc ggtccttccc
      421 tggaacttgg ggtgcggcat tccattctgg atgcttgagt caagatcgat gttcacaatg
      481 tccccctcgt ggaactcctg gatcgccctg ctgatggggg ataatccttt ctctctgact
      541 gttttcttta atttatatcg tgattttctt cttgttccgt gggtttttgc cataatattt
      601 ctccgaacta tgattgttat tttatcctac tttaattact tcaagctcaa taacttttgc
      661 ctcacttcct gtcaatgaag ctacactggg tatcgtacgc ccgccatcgc cagaaaccag
      721 ttccttgaca tagagtccgc cttcacaatt aatgatgagg acagcagtat tttcatcata
      781 cgattcaagt tcaactgagt gaaccttgcg tattcgttcc aggtcggcgc gtctgtgaag
      841 cactctggtg ggtgttttct gtttgatgag agttttgctt attacgttaa tcgatgattt
      901 aagtttttcc tctgttgtat tgtgtgtgat cttgagacga tattctttat ccactttcat
      961 tgattttagc tgttcaacag tttcactctc aacgaatttg agtccaagaa cttctatctt
     1021 gccctgtgcg ccccttttaa tttgcgcttc aagctcataa aggtctaagc tccttatgtg
     1081 aggtcttttt gcctcaacta caaatggcct gccatctcca agcataaggg catcaatatc
     1141 ctcacgcccc gccccgtgga aagcagtatc cgcacacctg gctgctgcta atagatgcgg
     1201 ttttatcagt tcatccactg attcctggta catctttccg gtattgtcgc acctctggca
     1261 cccttttcca ccgcattccc tgcatggcca tctggtctgg ggtattccgc gaacgagttt
     1321 ccgatacctt ccatagatat aaagggaatt tatttcaacc tcaacattat cattttcaag
     1381 atcaagaagc gccacaacct gcggtttctt gaggttggcc ttcttgcctg taagtttttc
     1441 tatccttttc cccacctccc ggttcaattc tgtcttcaaa ggttcggcaa atgttgtccc
     1501 gctttctgcc cacaggatct cctcattttc ggcaaggagt cctgtaacct ttgttccaac
     1561 caggaaattg ttatattcat aatccgatag cgcctgaact gactttgtta cccatgaatc
     1621 aatatttctg aaaagatcgt tgcaaaccca gcatttcatt tcaccgctgt tcctggcaag
     1681 ctttttcttc aattcctcat ctttttctgc tcccgcgtgc atcgcaagga caagtttcag
     1741 ggaggcgccg cgcatgtcat tggaaagtcc cgtggaaagt tttgcaaatt gccggcccag
     1801 gcagtgatcg catatcggac cttcatgcat tatcttacgg gctgtatcaa ggattatcat
     1861 ctggctcaca ttatgcgcgg tgttctaaat atgtttctgt ccatttcatt atgcaataac
     1921 acaatgcagt ggtcagcgtg caaagataga ggccctatac tcattatttc aggatcatat
     1981 cccaggacta ttctctcctc ctccacggtc aaacccagat ggtccccaag cacaaataaa
     2041 ggatcaaaat cttcatcgat tttttttttg cggatatctt ccccgtcttc ccgtaaatag
     2101 atgatcttct tatccatttc ctttaaaagg ccctccatat ccccttttct tacactgacc
     2161 ccgggtgttg actctgtcca gaaatcctgt atgttctttt cgagcgcctt ttttatcaat
     2221 gatgctgcgc ttcgttcatc agggctcagg tatctgactg cattgccttc aaaacggacc
     2281 aattttggag ggtatgaccc acctttgtgt acaagaaata cacgaacatc ccttcttaag
     2341 tcgtgcgaaa gaaatagcgc agcattaaca cacctgcaaa gaatgtccat acgtccggca
     2401 actcccggaa ggtcattgag ggaaaatgtt tcagtgactg ctttatgtcc tatgattata
     2461 aaatctttca tgagacgttg aaatgcatca gaaaaactta taactttcat tttccattta
     2521 gatgaggtga tattttctgc ccaagaaagc tttaataagc gtttctgata aaaatggtat
     2581 aattgagttt gcccaggggc ttgccaggtt tggatttgag attatttcga cgggcaggac
     2641 tta
//
LOCUS       DATGBN010000267         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_486806, whole genome shotgun sequence.
ACCESSION   DATGBN010000267 DATGBN010000000
VERSION     DATGBN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486806"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..934
                     /gene="cbiT"
                     /locus_tag="VKL21_06505"
     CDS             413..934
                     /gene="cbiT"
                     /locus_tag="VKL21_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HMB45455.1"
                     /translation="MLYPTGTPTQPEIIAIVLSKLDIKSTEVFVDIGCGSGSGSITAA
                     KLAKKVFSIDNREDAIMATSANIKECDINNISVLKGDASTLLPDIEMDCAFIGGSKNI
                     KQVIEILIRKPHVRFIVSAVKIETVAIALETMKKNNVFKELLQIQLSRGNELAGGTMF
                     KPENPIFLIVGGP"
     gene            934..2268
                     /locus_tag="VKL21_06510"
     CDS             934..2268
                     /locus_tag="VKL21_06510"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: protein motif:HMM:TIGR01465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HMB45456.1"
                     /translation="MLIGIGLGPGNPDLLTLAAIKALKESRRVFVPGKLAEKLVLPYA
                     KAQILDFPMIQDKKELSKLWDKNAAIVANEAKKGIVSFAVLGDPNVFSTFSHLKRTMQ
                     AKYPEIEISTIPGVSSVTAFAARTNTSIESSFIVSDGSPVKTKIILKSKHPEEAKIAL
                     MEEGFNEFIFAQRLFMHNEMVTDQIPQTGDYFSLMVANRGKNQPNNNVNKKIIYFVGA
                     GPGNPKYITVRGRELLESADFVMYAGSLVNPVVLNYVNGEKLDSFGMKLEDIADVLSK
                     KVEEGKTVVRLHSGDPSLYGAIIEQMDKLKKQGIEIEIIPGVTSLFAAAAALKTQLTI
                     NGITETLIITRPAGTTLEKDSIRELSRHNATMAIYLGTDKLRNIMKEVEYPEETPAAV
                     VYHASWEDELVILGTVGDIADRVEAEGIARSAMIIIGNVLTPLDFKRSHLYG"
BASE COUNT          762 a          458 c          479 g          656 t
ORIGIN      
        1 tttatctacc atatctatac ctcgtctata tttttgtttc atatttctac ctcgacttat
       61 tttattcaat ttatatgtat tttattctta tttttattta aattccgatt gagtcatctc
      121 ccactcaacc tttagaaaaa ataaaaaata cccaattcat ttactttgtc atctcccaat
      181 tatctatcac atcacataga ttcccggcaa tttatttttt gcggatatcg gcttttaata
      241 atatcattcc atctccctat tttcaaaaat acccaaattc cctaatataa agttcaatcg
      301 aaaatatata tatcttttca tgctcatgta tgattgatta gacagttttc cggataatct
      361 gcttacataa aagtttttat gctaaaactg ctgtttttag aagatgatta ttatgctcta
      421 tccaactgga actcctacac agcccgaaat aatcgcgatt gtattatcaa agcttgatat
      481 caaatctaca gaagtattcg tggatattgg ctgcgggagc ggttctggtt cgataactgc
      541 ggcaaaattg gcaaaaaaag tattttccat tgacaacagg gaagatgcca ttatggccac
      601 atctgccaat ataaaagagt gtgatataaa taatatctcg gtgttaaaag gagatgccag
      661 cacactgctt cccgatatag agatggactg cgcgttcatc ggaggaagca aaaatatcaa
      721 gcaggttatt gaaatattga taagaaaacc acatgttagg tttattgtaa gcgctgttaa
      781 gatcgaaacc gttgccatcg ccctggaaac catgaaaaaa aataatgttt tcaaggaatt
      841 gctacagata caattatcaa ggggaaatga gcttgcaggc ggtactatgt tcaaaccaga
      901 aaatccgatt ttccttatag ttggtggccc ataatgttga tcggcatcgg tcttggtccc
      961 gggaaccccg accttcttac cctggcagca attaaagccc tgaaagaaag caggagggtt
     1021 tttgtgcccg gaaaactcgc agaaaagctt gttttgcctt atgctaaagc ccaaatcctt
     1081 gattttccca tgatacagga taagaaggag ttgtcaaaat tatgggacaa aaatgcagcg
     1141 atagtggcaa atgaggcaaa aaaaggaatt gtttcatttg ctgtcctggg cgaccccaat
     1201 gttttctcaa cattctcgca cctaaaaagg acaatgcagg caaaatatcc tgaaatagaa
     1261 atttccacaa ttcctggtgt gagttcggtc acagcctttg cagcccgtac aaataccagc
     1321 atcgaaagct ccttcatagt aagtgatggg tcgcctgtaa aaacaaaaat aattctcaaa
     1381 tcaaagcacc ctgaagaagc aaaaatagca ttgatggaag aggggttcaa tgaatttatt
     1441 tttgcccagc gcctgtttat gcataatgaa atggttacag accagatccc tcaaaccggc
     1501 gactatttta gcctgatggt agcaaatcgg ggaaaaaacc aaccaaataa taatgttaat
     1561 aaaaagatca tttattttgt gggagcagga cccgggaatc caaaatatat caccgtcagg
     1621 gggcgcgaac ttcttgaatc cgcagacttt gtcatgtatg caggttcgct tgtaaatcct
     1681 gtggtcttaa attatgtaaa tggcgagaaa cttgatagct tcgggatgaa acttgaagac
     1741 atcgctgatg ttctttcaaa gaaagtggaa gaaggaaaaa cagtggtcag gctgcatagc
     1801 ggtgaccctt cattatacgg cgctatcatc gaacagatgg acaaactgaa aaagcaggga
     1861 atagagatag agataattcc cggcgtgacc tcactgttcg cggctgccgc tgcattaaaa
     1921 acacagctta cgataaatgg cattacagag actcttatta tcacaagacc ggcaggaaca
     1981 acgcttgaaa aagattcgat ccgggaacta tcgcgccata atgccacaat ggcaatatat
     2041 cttggaacgg ataaactcag gaatatcatg aaagaggtgg aatatcctga ggaaacaccg
     2101 gccgcagttg tgtatcatgc ctcatgggag gatgaattgg tgatccttgg gacagtgggc
     2161 gatattgcag atagggttga agcagaaggg attgccagat cagcaatgat aattatcgga
     2221 aatgtcctga cacccctgga tttcaagagg tctcatcttt atgggtgaac ctgcaataat
     2281 cactttttca aggaatattg acctggcaaa aaaaataaag gatgttactg gcggagatat
     2341 attaccatac tcagg
//
LOCUS       DATGBN010000268         4378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_292606, whole genome shotgun sequence.
ACCESSION   DATGBN010000268 DATGBN010000000
VERSION     DATGBN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292606"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VKL21_06515"
     CDS             <1..702
                     /locus_tag="VKL21_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HMB45457.1"
                     /translation="VHKLGFIEDVHKLGFKEEEKKSKIIEVKTLKENTDPVKKSLFHK
                     IVVIGSSTGGPRALQQVIPMLPSNLHAPVLVVQHMPPGFTKSLAERLNSQSVIKVREA
                     EEGDILQSGTVYIAPGDYHMIIKQQKINGDLKEVIALTKGERVQGVRPSVDVLLNSVA
                     PIFGQNSLGVILTGMGSDGTDGIRKLKLAGGKVMAEDESTCVVYGMPRSVIEQKLADF
                     ILPIGRIAEKIAEIT"
     gene            714..2663
                     /locus_tag="VKL21_06520"
     CDS             714..2663
                     /locus_tag="VKL21_06520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HMB45458.1"
                     /translation="MEDMSEYKEMFAVESAEHLQSMNEALLHLEKDPHNSDTINIMFR
                     AAHTLKGMSATMGYSNIKELTHNMENLMDRVRKNEIQLESSSIDILFKCLDTLEMMVE
                     TPEKSSEIDISSLIEKISLLSKNDSAPNREKDLSTENAVHDMAKNDTPNIAPRIPEIN
                     GCNIFEINVTLHESCLLKSARSTVVMRNLSELGEIIETVPPIKDLEDEKFEHEFKVII
                     STKEDAKKLEDAAKKVSEIVKVEVKSHQGSNTKAKTEEKNSSNEGGKNSIKSVQSVRV
                     SIERLDSLMNLVGELIINKIRLMQLASVYKLDALEEALASLNRLTNDLQEEIMASRMV
                     PIDQIFNRFPRMIRDLAKKEEKEIDLILEGGDIELDRTVLDEIGDPLVHILRNCVDHG
                     IELPEVRKQNGKDPKGTVKLIARREKNHVVIEAIDDGKGMDPQKMRESAVTKGIMTQE
                     EASKLSDNEAINLSFLPGFSTAEKVTDISGRGVGMDVVRTKIGGMGGSIKLESVLGKG
                     TTITLKLPLTVAIIHSLMVKVGTDIYAIPIANVIRDLSIKKEDIKTIKGEEVILIRGE
                     VLPLIRLHKLFDIKTNGSEELLVVVVERAGSNVGLIVDQVIGQQEVIIKNLDNNILKG
                     VKGFAGATILGDGNVALILDVVTLL"
     gene            2660..3502
                     /locus_tag="VKL21_06525"
     CDS             2660..3502
                     /locus_tag="VKL21_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HMB45459.1"
                     /translation="MTNLTPPSTIHEDDREFTELKAIIKRKIGFNCEDYKQPHLKRRL
                     AVRLRATQTTSYKDYIKVLLKNADEEQQLKETLTVNVTELFRNPETYESVRTNVLPEL
                     IKYKGNNKIIKVWSAGCSNGEEPYSIAILLNEYLGISAKRYNISILATDIDEDSLGKA
                     ENALFQSKQLEKMGKERIGRFFVMKDNNYQVIDEFKKLVKVKRHDLISGPKLSGFDII
                     FCRNVTIYFEQKLQEILYMNFYNALNEGGYFVMGKTETLVGPASQLFKRFDLKERIYK
                     KEKL"
     gene            3499..4116
                     /locus_tag="VKL21_06530"
     CDS             3499..4116
                     /locus_tag="VKL21_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HMB45460.1"
                     /translation="MTDLRTLTDFQADALKEVGNIGIGHATTSLSQMVNKKVGISLPE
                     LKLLPLIKVPILVKNEEPVIGIILELKGDAKGFMLLLLSKKTAKYLIKLVIGETDETK
                     GFDEMETSVLKELGNIMGGTYISSLSNFLSISIGLSTPSDIYDMSDAIINQVVCLMSP
                     DVEDVLFLKTEFDINSEKIDGKILIFTDSASLTKMLDAINKMVGK"
BASE COUNT         1486 a          761 c          989 g         1142 t
ORIGIN      
        1 gtccataagc ttgggtttat tgaagatgtc cataagcttg ggtttaaaga agaagagaaa
       61 aaatccaaaa tcattgaagt caaaactctg aaagagaata ccgaccccgt caagaagagc
      121 ctatttcata aaattgtagt tatcggatca tctactggcg gcccaagggc attgcagcag
      181 gtaattccca tgttaccctc caacctgcat gctccagtcc ttgttgtaca gcatatgccc
      241 ccaggattta ccaaatccct ggcagaacgt ttgaattccc aatccgtgat caaagtaaga
      301 gaggccgaag aaggtgatat tctgcaatcc ggaactgtat atatcgctcc cggagattat
      361 catatgatca taaaacagca gaaaatcaat ggcgatctta aagaggtaat tgccctgaca
      421 aagggtgaaa gagtccaggg tgtgcggcct tctgttgatg ttcttctcaa ttcggtagcg
      481 ccgatcttcg gacagaactc actcggcgta atacttacgg gaatgggttc ggatggaact
      541 gatggcatca ggaaacttaa acttgcaggc ggcaaggtga tggcggagga tgagtcaacg
      601 tgcgtggttt atggcatgcc aagatcggta attgaacaaa aactggctga tttcatttta
      661 ccaattggca gaatagcgga aaaaatagcg gaaattactt agaggaaata tttatggaag
      721 acatgtctga atataaagaa atgttcgcgg ttgaatctgc ggaacatctt caatcgatga
      781 atgaggcgct tttgcatctt gaaaaagatc cgcataacag tgacacaata aatataatgt
      841 tcagggcagc ccataccctc aaaggaatgt ctgcaaccat gggttattcc aatattaaag
      901 aactcaccca taatatggaa aacctgatgg acagggttcg caaaaatgaa atacagcttg
      961 aatcttcttc aatagatatc ctgtttaaat gtcttgatac ccttgaaatg atggtggaga
     1021 caccggaaaa atcctctgaa atcgatattt catctcttat tgaaaaaatt tcattgttgt
     1081 caaaaaatga ttctgcgcca aatagggaaa aagacctttc aactgaaaat gcggtgcatg
     1141 acatggcgaa aaatgatacc cccaacattg cacccagaat cccggaaata aacgggtgta
     1201 acatttttga aataaatgtc acacttcatg aatcatgttt gttgaaatct gcccgctcaa
     1261 ccgtcgtaat gagaaattta tcagagcttg gcgagatcat tgaaactgtt cctcctataa
     1321 aggatctgga agatgaaaaa ttcgagcatg aattcaaggt tatcatatca acaaaagaag
     1381 atgctaaaaa attagaggat gctgcaaaaa aagtatctga gatagtaaaa gttgaagtta
     1441 aatcccacca gggttcaaac acgaaggcaa aaaccgaaga aaaaaattct tcaaatgaag
     1501 gcggcaaaaa ctctattaaa agcgtacaga gtgttcgggt aagcatagaa cgcctggatt
     1561 cccttatgaa ccttgtaggt gaactcatca taaacaaaat tcgattgatg cagcttgcca
     1621 gcgtgtataa attagacgct cttgaagagg ctcttgccag tcttaatcgg cttacgaacg
     1681 atctacagga agaaataatg gcatcaagaa tggtgcctat tgatcagatt ttcaaccggt
     1741 tcccgagaat gatccgggat cttgcgaaaa aagaagagaa agaaatcgat ttgatattgg
     1801 aaggggggga tattgaactt gatcgaactg tactggatga gatcggagat cctcttgtac
     1861 atatcctgag gaattgcgtg gaccatggaa ttgaattacc cgaggtaaga aaacagaatg
     1921 gaaaggatcc gaaaggtacg gtcaaattaa tcgccaggcg ggaaaaaaat catgttgtta
     1981 ttgaagcgat tgatgatggt aaagggatgg acccccaaaa aatgagggag tctgctgtaa
     2041 ctaagggaat aatgactcag gaagaggcat caaagctttc ggataatgaa gctatcaacc
     2101 tgtcattttt gcctggattc agtactgctg agaaagttac ggatatctca ggaagaggag
     2161 ttggaatgga tgtcgtgagg acaaagatag gcggcatggg gggttcgata aaacttgaat
     2221 ccgttttagg gaaggggaca acgataacac tgaagcttcc attgacggtc gctataatac
     2281 attccctgat ggtaaaagta ggaacggata tctatgcaat accaatcgca aacgtgataa
     2341 gggacctgtc aattaagaaa gaggatataa agacgatcaa aggtgaagaa gtaattttga
     2401 tcaggggaga ggtactgcct ttaattcgat tacataaact cttcgatatc aaaactaatg
     2461 gttcggagga actccttgta gttgtagttg aacgcgccgg cagtaatgtg ggacttatag
     2521 tggaccaggt catcggccag caggaagtta taatcaagaa tcttgataac aatattctta
     2581 aaggggtcaa aggttttgca ggcgccacga tccttgggga tggcaatgtt gcattgatac
     2641 ttgatgtggt gacattacta tgacgaattt aaccccgccc agtactatac atgaagatga
     2701 cagggaattt acggaattaa aggccatcat caaacgaaaa atcggattta actgtgaaga
     2761 ctataaacag cctcacctga agagaaggct tgctgtaagg cttcgggcga ctcagacaac
     2821 atcctataag gattatatca aggtgctttt aaaaaatgca gacgaagaac aacaactaaa
     2881 ggaaacatta acagtaaatg tcacggaatt attcaggaac cctgaaacat atgaatcagt
     2941 ccggaccaat gttcttcctg aattgatcaa gtacaaagga aataacaaga tcatcaaagt
     3001 ctggagcgca ggatgctcaa atggtgaaga accttattcg atcgcaattc ttttgaatga
     3061 atacctggga atttccgcca aaagatataa tatttcgatc ctggctacgg atatcgatga
     3121 ggactccctt ggaaaagcag aaaatgcctt attccaatca aagcagctcg aaaaaatggg
     3181 aaaggaaagg atcggccggt tttttgttat gaaggataac aattaccagg tcattgatga
     3241 atttaagaag ctggtgaaag tgaaacgcca tgatctgata tccgggccga aattatcggg
     3301 attcgatata atattttgca ggaacgttac aatatatttt gaacaaaaat tacaggagat
     3361 attgtatatg aatttttata atgctctgaa tgaaggggga tattttgtta tgggaaagac
     3421 agaaacactt gtggggccgg caagccaatt gtttaaacgt tttgatttga aagaaagaat
     3481 atataagaag gaaaaattat gactgattta agaacactca cagattttca ggcagacgcg
     3541 ctcaaggaag ttgggaatat tggaataggg catgcaacaa cttctctttc ccagatggtc
     3601 aataaaaagg tagggatatc gttaccggaa cttaaactgt tgcctttgat caaggtaccg
     3661 atacttgtga aaaatgaaga gcctgttatc gggataattc ttgaacttaa aggcgatgca
     3721 aaaggcttta tgctcctttt attgtctaag aaaacggcaa agtatttgat aaaacttgta
     3781 attggggaaa cagatgaaac aaaaggattc gatgaaatgg aaacttctgt tctaaaggaa
     3841 ttaggaaata ttatgggggg tacatatatt agctctcttt caaatttcct ttccatatcg
     3901 atagggctgt ccactccatc agatatatac gatatgtcag acgcaataat caaccaggtt
     3961 gtatgcctta tgagcccgga tgttgaagac gttttgttcc tcaagaccga atttgacata
     4021 aattctgaga aaattgacgg gaaaatattg atatttacgg attccgcttc ccttacaaag
     4081 atgctggacg ctattaacaa gatggttggg aaataaggga ttttcctttt cattttattt
     4141 ctttatttaa tttccgacca ggttagtttc tcacttggtc tttagaaaaa ataaaaatat
     4201 ggaaaattta tctggcgatt attacaataa cgggttcaaa ggggcaagaa agctcctggc
     4261 tttcgccgtg gagatgaatt gcccctttgc atgaacacga tgctcaccca aaaagcattt
     4321 gcgcgaacgg atttcagggt gagcaaaata tatatatgaa ctcacagcat attaatat
//
LOCUS       DATGBN010000269         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_448415, whole genome shotgun sequence.
ACCESSION   DATGBN010000269 DATGBN010000000
VERSION     DATGBN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448415"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VKL21_06535"
     CDS             complement(<1..931)
                     /locus_tag="VKL21_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HMB45461.1"
                     /translation="MALDLGITIGLFIIFLLVLIGPFKIKIIEQNLEVFLFICGVLAL
                     TISHFVTLAGMETGWRWSIIEEALVAPLYISNSLHLPIGIVQVVLVVGLIIHKWHKPI
                     HGAIRTMAEKLSLKVMAFILVAVLGLFSSVISAILAAIILVEMVNALPLSRKSKVDLT
                     VISCFSIGLGAALTPLGEPLSTIAISKLSGPPYYATFTYLFDQLAIYVIPGIIAFGFV
                     GMFFLGKVNPNDEGLKAEDYNETVKDVIMRAVKVYVFIMALVFLGDGFKPIIFEYIAK
                     LPAEALFWVNMVSAILDNATLTAAEIGPTFIQIQ"
     gene            1114..1440
                     /locus_tag="VKL21_06540"
     CDS             1114..1440
                     /locus_tag="VKL21_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HMB45462.1"
                     /translation="MIAELLSDYITQFISSIGYLAVLIAGLIFHYGRASLKGLHINAA
                     LMGLLIAGVMLIPGNIPNIISANKLGITSKEWAKLGLPLGAVIMVVYYIILFGFYSQF
                     SISMNL"
     gene            complement(1413..2063)
                     /locus_tag="VKL21_06545"
     CDS             complement(1413..2063)
                     /locus_tag="VKL21_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HMB45463.1"
                     /translation="MSIEIEPLFTIIGEVVVYLAAFLLILAIVGSFLIGYSFKTEKFI
                     FPGFMLFSITLLETLVKALFRFIKMDDSIVDNVGITLKNKISLRKFRDTPINERLIFF
                     PQCLRALTCPSKLSSEGMQCISCGACEIGRAKKTAEMLGYKVYIVPGSSFIRRLVLKH
                     KPSAILGVGCMTEIKAGLEMCEKIQLFGVGMVLEKAGCVSTVLDWDKFYKFIEMEN"
     gene            complement(2114..3097)
                     /locus_tag="VKL21_06550"
     CDS             complement(2114..3097)
                     /locus_tag="VKL21_06550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMB45464.1"
                     /translation="MKPLFDLGVLPIRHKTSKGFKPHISLVFNAGKRLTFSVDTTNSR
                     SMQPDAYLITHAHSDHHGRSAMLSKNAICSHETAKALEILYGKKYAGRTFKIGDSIDI
                     AGVDVKTYPTHHTIGSCAFYWKNDVGARIMVTGDVKDSKDLPNCDCLVTEANYGDPDD
                     PKCHFEDDLGAFRESLEETHNDIAFGAYSFGKAQRAVRLIRESGYSGQIGMDAVSHAL
                     TDGLMDDAGQLVDLDSDCGIRITTPGEIPDLSSARKFILTGRRDIKFPTINISDHMDV
                     NGLIGMTKQCAPSAVITYHPGGNRPLKLAAYLRKNGLYAKALEEINTILEI"
     gene            complement(3108..3380)
                     /locus_tag="VKL21_06555"
     CDS             complement(3108..3380)
                     /locus_tag="VKL21_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMB45465.1"
                     /translation="MSKVVLMDFFAEWCGPCKMQDPIMEELKKIFGDKVEFKKVDVDN
                     NNELAAKFMVHAVPTLVIEKDGAVFKRYTGVTRANVLEADLNAALK"
     gene            complement(3452..3922)
                     /locus_tag="VKL21_06560"
     CDS             complement(3452..3922)
                     /locus_tag="VKL21_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMB45466.1"
                     /translation="MIIVKIGDTAKDFTLSDQDGNEFHLYSLKGKRVLLSFHPLAWTK
                     VCAEQMKSLEKNRERFEKLNTVAVGMSVDTVPSKKAWAKELGIKNIRLISDFWPHGKI
                     ADLYGIFREKEGTSERANIIVDENARVVFVRVYPISQLPDIEEIIKVLEDLKPK"
BASE COUNT         1234 a          899 c          747 g         1280 t
ORIGIN      
        1 tctgtatttg tataaaagtt ggccctattt cagccgcggt cagcgtggcg ttatcaagta
       61 tagcagagac catattaacc cagaagagtg cctctgcagg caatttggcg atatattcga
      121 agataatggg tttgaaacca tcaccaagga aaacaagtgc cattataaat acatacactt
      181 ttacagctct cattattaca tcttttacag tttcattata atcctcagct ttcaagcctt
      241 catcgtttgg atttactttt cccaggaaga acatgccaac aaaaccaaaa gcaataattc
      301 cggggataac gtaaattgca agttgatcaa acaggtatgt aaaagttgca taataaggag
      361 ggcctgataa cttggaaata gctattgtag aaaggggctc accaagaggc gtaagcgcag
      421 cgccaagacc gatcgaaaaa catgatatga ccgtaaggtc caccttggat ttccttgaaa
      481 gcggcaaggc attgaccatc tccacaagta tgatcgcagc aagaatagcg gatattacac
      541 ttgagaacag gccaagtact gcgactagta tgaaagccat gactttcaat gaaagttttt
      601 cggccattgt ccttatcgct ccatggatag gtttatgcca tttatgaatg atcaatccca
      661 cgaccaatac aacctgaacg atccctatgg gtaaatgcag actgttggaa atatataaag
      721 gtgcaaccaa tgcttcttct attattgacc agcgccagcc tgtttccatg cctgccaatg
      781 taacaaaatg tgaaatagtc agtgcaagta ccccgcaaat aaacagaaat acctcaagat
      841 tttgctcaat gatcttaatt ttaaaaggac ctatcaatac taacaaaaat attataaaaa
      901 gccctattgt gattcccagg tcgagtgcca ttttattatt ttcctcctat aacatcctaa
      961 aacaaatcca gccaaactgc tgaatttttg tgtatatttt tcacaggttc ttttatagtt
     1021 tgttatttga tttgattaat aatagtgaaa aatcgcgtgc atgctaattt caagaagcgc
     1081 aatgtttaaa atttcttatt ccataaggct attatgatcg cagaattgct atctgattat
     1141 atcacacagt tcatttcatc aatcggatat ctggctgttc ttatagccgg actgattttt
     1201 cactatggtc gcgctagtct gaaaggctta catatcaatg cggctctcat gggtcttttg
     1261 atcgcaggag ttatgttgat cccggggaac attcccaata ttatctccgc aaataaactt
     1321 ggtattacaa gcaaagaatg ggcaaaactc ggtttgcctc tcggggctgt aataatggta
     1381 gtttattaca taatcctgtt cggattttat tctcaatttt ccatttcaat gaatttgtag
     1441 aacttatccc agtccagtac agtggaaaca catcctgcct tctcaagaac catacccaca
     1501 ccaaaaagct ggattttctc gcacatttca agccctgctt ttatttctgt catacatccc
     1561 acacccaata tcgcagatgg cttatgtttt agcacaagcc gccggataaa acttgagcct
     1621 ggtacaatat agaccttata accaagcatt tccgcggttt tcttggctct tcctatctcg
     1681 catgcaccgc aagatatgca ctgcatgcct tcagagctca gtttggatgg gcaggtgagg
     1741 gctcttaagc attgtgggaa aaaaatcaat cgttcattga taggagtatc cctgaatttc
     1801 ctgagagaga tcttgttttt tagcgtaata cccacattat caacaatgga atcatccatc
     1861 ttgataaacc tgaataatgc ctttaccagg gtttcaagaa gtgtgatcga aaaaagcata
     1921 aatcccggaa aaatgaattt ctcggtcttg aatgaatatc ctataagaaa tgagccaacg
     1981 atcgctaata ttaataaaaa agctgctaaa tatactacaa cttcgccgat tattgtgaat
     2041 aaaggttcaa tttctattga catttttgta atatgaatat tttccccggc taaaaggtta
     2101 tctgaaggat ttgtcaaatt tcaagaatag tatttatttc ttcaagcgct tttgcgtata
     2161 aaccattttt ccttaaatat gcagcaagct taagagggcg gttgcctccc gggtggtatg
     2221 taatgactgc tgacggggcg cattgctttg tcattccgat aagaccattc acatccatat
     2281 ggtcactgat attgatggtg ggaaatttaa tatcccttcg tcctgtcaat atgaatttcc
     2341 ttgctgacga aagatccggt atttcacccg gtgttgtgat cctgatcccg caatcgctat
     2401 caagatcaac aagctgcccg gcatcatcca ttaatccatc agtgagcgca tgagataccg
     2461 catccatccc gatctgtccc gaataacccg attcacgaat aagtctgaca gcgcgctggg
     2521 cttttccgaa agaataagcc ccaaaggcaa tatcattgtg tgtttcttcc agggattccc
     2581 tgaatgcgcc aagatcatcc tcgaaatggc atttagggtc atcaggatca ccataatttg
     2641 cctccgtgac aagacaatcg cagtttggca agtccttaga atccttgaca tcacctgtaa
     2701 ccatgatcct tgcgcctaca tcatttttcc agtagaaagc gcatgagcct attgtatgat
     2761 gggtcggata tgtttttacg tcaacacctg cgatatcgat actgtcaccg atcttaaatg
     2821 ttcttcccgc atattttttc ccataaagga tctcaagcgc ttttgcggtc tcatgagaac
     2881 atatggcatt tttcgagagc attgcagatc tgccatgatg gtccgaatgt gcatgggtta
     2941 taagatatgc atcaggctgc atagaacggg aattcgtggt atccacggaa aaagtcaacc
     3001 gtttgcccgc attaaaaaca agtgaaatat ggggcttgaa tcccttactg gtcttatgtc
     3061 tgatgggcag gacaccaagg tcaaataaag gtttcaatca aaccacttta tttcagggcc
     3121 gcgtttaaat ccgcttcaag cacattcgcg cgagttacgc ctgtatatcg cttaaaaaca
     3181 gcgccatctt tttcaattac caatgttgga acagcatgta ccatgaattt agccgctaat
     3241 tcattattgt tatcaacatc aactttctta aattcaactt tatcaccgaa tattttctta
     3301 agctcttcca tgatcgggtc ctgcatcttg cagggaccgc accattctgc aaaaaaatcc
     3361 attagaacga ctttgctcat ttaatttcct cctctggtat atacttgcta ggatacctaa
     3421 taataccctt atgatttaaa attattgtcc attattttgg cttcaaatct tcaagaactt
     3481 ttatgatctc ctcaatatca ggcagctgtg aaatgggata aaccctgaca aataccaccc
     3541 ttgcgttttc atcaacaatg atatttgccc gttcggaggt cccttctttt tccctgaata
     3601 tcccatagag gtccgcaatt ttaccatgag gccagaagtc cgaaataagc cggatatttt
     3661 ttattccaag ttcttttgcc catgcttttt ttgaagggac cgtatccacg ctcataccca
     3721 ctgctaccgt attcaatttc tcaaatcttt cccggttttt ttcaagcgac ttcatctgct
     3781 cggcgcagac ttttgtccag gcaagcggat ggaaagataa caatactctt tttcctttca
     3841 gggagtatag atgaaattca ttgccatcct gatcgctaag agtaaaatct ttagccgtat
     3901 ctccaatttt cactatgatc attctttaac ctccttatta ttttacaaaa atacactccg
     3961 acagcaatta gtaatatttg atcttcttat attaaatatt gtcaatcaga cagatttaaa
     4021 tagtaaactt caggttttcg acaaatttat tttctggcac tcaaattata tattataaaa
     4081 ttaaaagcag aatagctatc atgatctcaa aagcgaaaat acaagataat ttatggaaca
     4141 atttatgggt tttttatctt
//
LOCUS       DATGBN010000270         7005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_525855, whole genome shotgun sequence.
ACCESSION   DATGBN010000270 DATGBN010000000
VERSION     DATGBN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525855"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VKL21_06565"
     CDS             <1..837
                     /locus_tag="VKL21_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMB45467.1"
                     /translation="IDAPVFHYYRTETYNKAICYLVRKYKPEVLLMGATGLGRDLAGA
                     VATKLNTGLTADCTGLDIDEKGYLLQTRPAFGGNIMATIFTERTRPQMSTVRPHVMLL
                     PQKDTSRKGEIVIESMMIKEEDVATKVLDVVKDQSAECDLGAAEVIVSGGRGMSSSEN
                     FTLLQQLADELGGVVGCSRAVVEAGWMPAERQVGQTGKTVRPKIYIACGISGAIQHLV
                     GMQSSDVIIAINQDKYAPIFEVATYGIVGDVFQVVPAIINNIRELRAQKSGSSDKFDR
                     KE"
     gene            841..1116
                     /locus_tag="VKL21_06570"
     CDS             841..1116
                     /locus_tag="VKL21_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45468.1"
                     /translation="MSQFMSLNISTIVFAAAIALFCWSCYRRFRLLLLGKAEDRFKDA
                     GRRIWGMLYYAFGQRCTVSHGYRFGWNHLVLFWSFMILLIANTEFLL"
     gene            1156..2844
                     /locus_tag="VKL21_06575"
     CDS             1156..2844
                     /locus_tag="VKL21_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HMB45469.1"
                     /translation="MDIYHPLAVLIEIASVLALLAVCIAIIRRLAFPPSYIEARSRDA
                     FIILGLIALLMLAFFGLHASEIAQGSEEAASYMPVSEFLASAFFSNVSPGSLNGYIDA
                     FWWLHAVVLLAFLNYLPYSKHMHILAGIPNVFFRSLTKVNTQPREEFKKGNAYGVGKI
                     DQFTWKDLIDSYSCTECGRCSDNCPATATGKLLNPRLVIHDMKVNLLKNGPLITKKKE
                     TVLPLIGTGNEGSIAEEVLWACTTCGACMEVCPVFIEHVPKIVDMRRNLVEMSAKVPD
                     ELLSLFENTEQRSNPWGIVPAERAKWAAQADVKTFVAGETEYLFYVGCFGAYDARSRK
                     VSIAISRILNSSGISWGILGKDELCCGDSLRRLGNEFVFDRMVQENIKIFKEKGIKKI
                     ITQCPHCYNTLKNDYRQYGVDLEVIHHTEFINDMIKSGKLKLNGAKDIGNLVFHDSCY
                     LGRYNGIYDAPRSVIASVTGKAPIEMERRNDRSFCCGAGGGRMWMEEGTGKRINVERV
                     EEALKKNPGTICVCCPYCMTMFEDGLKGLKADDRVQVLDLAEIVSEALKSATSR"
     gene            3079..4020
                     /locus_tag="VKL21_06580"
     CDS             3079..4020
                     /locus_tag="VKL21_06580"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMB45470.1"
                     /translation="MRIGRIIILLIFMSVAFSTAAYAAEANFSASLLTANDVNCKKCH
                     ADPPHIIHANKPVECVDCHGPKLNVSTPQCTKCHNGPIHQVHAGKVATQTCAYCHKDI
                     AGVHNNLISDAVYSHCHKDLIDVHGKDAACVKCHKSPPEIVKPIKSEGMVLICQDCHD
                     ASSVATIHAPADEKTGCYECHKGTSKANGSEIPHQIHASKVDCKSCHQENQQVVVPKC
                     TRCHDIDQLHAFNKIGKLTSQSGLKCSACHTDETKLSGPAPTQPEKTVVSPAVTVVKE
                     NTTRTPEAPAAKIPGFEAISATGLLIAVYMTRKRGLN"
     gene            4208..5857
                     /locus_tag="VKL21_06585"
     CDS             4208..5857
                     /locus_tag="VKL21_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45471.1"
                     /translation="MNSSNVKRDIFGRIALVCSATTAVLGVLGMIGWLSGFRDVTGFG
                     SYYSYRSPFMALEFVLLGCVWFFYVFNPSQRFRKVFALVLAFLILLIALFNLIQFFNS
                     AQIGIENKLLVLEVDSATLMSPISDAFFLLDCSAMILLLLGHPRRVAGLSGGLSFIVI
                     SVNLVILLGYLYGAPLLYGGEIRPVELPVGVAFIVLSTGLIATSGEDYSLLKLILGPS
                     VRALLLRYFLLVILIVALTDGVFYNILSQFKLKITISELQGLSALIYVIAVSAVVFQL
                     SHIIGNALYRSETARKQAEDLNELLAREISNRERAEDELKQRAIELARSNAELEQYAY
                     IASHDLQEPLRMITSFTQLLEKRYKGRLDNDADEFIGHIIDGATRMQMMINGILAYSR
                     AGLSVKPFELTDCETVINQALDNLKIAIKESGAIVIHDRLPSVMADSSQMTTLIQNLL
                     TNAIKFRKETPRIHVCAKQKRGEWIFSVQDNGIGISPEFIDRIFVLFYRLHSRDAYPG
                     SGIGLATCKKIVERHGGRIWVESKLGKGSTFYFTIPTKRGE"
     gene            5862..6305
                     /locus_tag="VKL21_06590"
     CDS             5862..6305
                     /locus_tag="VKL21_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45472.1"
                     /translation="MEKMKKRVEILLVEDNPGDIRLTVEALKENTMHNKLNVVNDGAE
                     ALTFLYKRSKYTDAPRPDLILLDLNLPKKNGLEVLEEIKKDPSLKRIPVVIFTASSAQ
                     RDILNSYNHHANCYITKPVHLEQFIAVVKSIENFWLTVVKLPQGD"
     gene            6307..>7005
                     /locus_tag="VKL21_06595"
     CDS             6307..>7005
                     /locus_tag="VKL21_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45473.1"
                     /translation="MEKIKVLLIEDNPGDARLIKEMLTEARSVSFDLEWKDNLSKGLK
                     LLTEKGIDVVLLDLMLPDSAGAETFAKTHACAPHAPILVLTGLDDETVAIGAVKEGAQ
                     DYLIKGHIDSNLLIHAVRYAIERMRIKEQLQESEMRYRTLFEQSPDGVLIINPLTASA
                     IEFNEAAHRQLGYTREEFKNFRIFDYEAKEKFDETKYHIEKLIRDGKDDFETLHRTKD
                     GKFRNVMVTVQKIEL"
BASE COUNT         1984 a         1453 c         1697 g         1871 t
ORIGIN      
        1 atagacgctc ccgtttttca ctactatcga actgagactt ataacaaagc catctgttac
       61 ctggtaagaa agtacaaacc ggaggtgctt ttaatgggcg caacgggtct gggaagagac
      121 ctggcaggag cagttgccac aaaactcaat acaggcctga ctgcagattg caccggtctt
      181 gatattgatg agaagggtta tttgctgcag acgcgccctg cctttggcgg gaatatcatg
      241 gcgaccattt ttactgagcg cacaaggcct caaatgtcta ctgtcaggcc acacgtaatg
      301 cttttgccac agaaggatac ctcgcgaaag ggagagatcg tcatcgagtc aatgatgatc
      361 aaagaagaag atgttgccac caaagttttg gatgtagtaa aagaccaaag tgcagaatgc
      421 gatctgggag cagccgaggt aatagtttcc gggggacggg gcatgagttc aagcgagaat
      481 ttcactttat tgcagcagct tgctgatgag ctgggaggcg ttgttggttg ttcgcgagct
      541 gtagtggaag caggatggat gcccgcagag cggcaggtgg gacaaaccgg caagacagta
      601 agacctaaga tatatatcgc atgcggcatc agcggcgcta ttcagcacct ggtaggtatg
      661 caaagttcag atgtgatcat cgctatcaac caggataaat atgccccgat ttttgaggtt
      721 gctacttatg ggatcgtggg agatgtattt caagttgttc cggccattat caataatatc
      781 agggaattac gcgcccagaa aagtggatca tcggataaat tcgacaggaa ggaatgattt
      841 atgtcacaat ttatgtcgct taacatcagc acaatagttt ttgcagcagc tatagcgctt
      901 ttttgctgga gttgttacag gcgctttcgt ctcctgttgc taggaaaggc cgaagatcgt
      961 tttaaggatg caggcaggcg tatatggggc atgttgtatt atgcatttgg gcagagatgc
     1021 actgtttccc acggatatcg ttttggctgg aaccatctgg ttcttttctg gtctttcatg
     1081 atactattga tagccaacac ggaattcctg ctttaggggc tgtttcccga ttacataagc
     1141 ttctctctgt tacctgtgga catataccac ccattggccg tcttgatcga gatcgcatca
     1201 gttcttgccc ttctggcggt gtgcattgcg atcatccgcc gtcttgcttt cccgccttca
     1261 tacattgaag caagaagcag ggatgcattt attattctgg gccttattgc tttgcttatg
     1321 ctggcatttt tcggtttaca tgccagcgag atagcgcagg gtagtgaaga agccgcttct
     1381 tacatgcctg tatcggaatt cctggcttcg gctttctttt cgaatgtttc accgggaagc
     1441 ctgaatggat atatcgatgc tttctggtgg ctgcatgcgg ttgtgctttt ggctttcctg
     1501 aattacctgc cttatagtaa gcacatgcat atcctggcag gcatcccgaa cgtctttttc
     1561 agaagcctca ccaaggtcaa tacccagccc agggaggaat ttaaaaaggg caatgcatac
     1621 ggtgtcggga aaattgacca gtttacctgg aaagatttga tcgactctta ttcatgtacc
     1681 gagtgcggcc gctgctcgga taattgtccg gctactgcaa caggtaagct attgaatccc
     1741 cgccttgtta tccatgatat gaaagtcaac ctgctgaaaa atggtcccct gatcactaaa
     1801 aagaaggaaa cggtattgcc attgatcggc acaggtaatg aaggtagtat cgcagaagag
     1861 gttctctggg catgcacgac ctgcggggca tgcatggaag tatgtccggt gttcattgaa
     1921 catgtcccga agatcgttga tatgcgccgg aatctagtgg agatgagcgc aaaagtacct
     1981 gatgaattac tatccctgtt tgaaaatacg gaacagcgca gtaatccctg gggcattgtg
     2041 ccggcagaac gcgcgaaatg ggctgcgcag gcggatgtaa agacttttgt ggctggtgag
     2101 actgaatatt tgttctatgt gggatgtttt ggagcatatg atgcgcgcag cagaaaagtt
     2161 tctattgcta tttccaggat actgaattca agcggcattt catggggtat cctgggaaaa
     2221 gatgagttat gttgcggtga tagcctgcgt cgcctgggca atgaattcgt ttttgatcgc
     2281 atggtacagg aaaacattaa gatattcaag gaaaaaggta ttaagaaaat cattacccag
     2341 tgcccgcatt gctataatac cctgaagaac gattatcgcc agtatggggt ggatctcgaa
     2401 gttatccatc acactgagtt tataaatgat atgataaaat cgggtaaatt gaagctgaat
     2461 ggggcaaaag acattggcaa tcttgtattt catgattctt gctatctggg gcgctataat
     2521 gggatatatg atgccccgcg atccgttatt gcttcggtta ccggtaaggc accgatcgaa
     2581 atggagcgcc gcaatgatag gtctttttgc tgtggcgcgg gaggtggaag aatgtggatg
     2641 gaggaaggca ccggtaaacg catcaatgtg gagcgggtcg aagaagccct taagaaaaat
     2701 cccggaacca tttgcgtatg ttgcccctat tgcatgacca tgtttgaaga tggattaaaa
     2761 ggcttaaaag cggacgacag ggtgcaggta cttgatctgg ccgagatagt aagcgaggca
     2821 ttgaagtcag ctacctcacg ctaaaagctg tgaggcttgt cattccagtt ttcgctaaag
     2881 agtttgttct ccatgaggcg catgatacaa atatcaagaa cggacgacaa taggctggtt
     2941 gacatcagga tatccgaccc ctactgagca gattatacca tgttactgtc ggagcaaccc
     3001 gataaaaaca aatcgaaaga ataatataca atgcacaaca cgaatgatta aaaacgggtt
     3061 atcggtggat gatttgatat gaggattgga agaattatta ttttattgat tttcatgtct
     3121 gtcgcatttt cgacagcagc atatgcagcg gaagcaaatt tttcagcatc tttactgaca
     3181 gcaaatgatg tcaattgtaa gaaatgccat gcagacccac cgcatattat ccatgccaat
     3241 aaaccggtgg aatgtgttga ctgccatggc cccaagctta atgtatcaac accccaatgc
     3301 acaaaatgcc ataatgggcc gatccaccag gtacatgccg gaaaagtggc cacgcagaca
     3361 tgcgcatatt gtcataagga catagcaggg gtacataata atctgataag cgatgctgta
     3421 tactctcatt gccacaagga cctgatagat gtacacggaa aggatgccgc ctgtgtaaaa
     3481 tgtcacaagt ccccgccaga aatcgtaaaa cccattaaat cagaaggaat ggtccttata
     3541 tgccaggact gccatgacgc ttccagtgtg gcgaccattc atgcacctgc ggatgaaaag
     3601 acaggatgtt atgaatgcca caaaggtact tcaaaagcaa atggcagcga gataccacat
     3661 cagatccatg cttcaaaggt ggattgtaaa agttgccatc aggagaacca gcaagttgtg
     3721 gtacccaaat gcacaagatg ccatgatata gaccagctgc acgccttcaa caaaatagga
     3781 aaattaacat ctcagtcggg attgaaatgt tctgcctgcc acacggatga gaccaaactg
     3841 tcagggccag cacctacaca accggaaaaa acagtggttt cacccgcggt aacggttgta
     3901 aaggagaata caacaaggac cccggaagct cctgcagcaa aaatacccgg atttgaagcc
     3961 atatctgcga caggactttt gatcgctgtt tatatgacaa gaaagcgggg cctgaattaa
     4021 caaaatcaat tgaaataaat cacttatctc attattagat tttgattctg gaatttttta
     4081 catcaaaatt cccgtctgta accttactgt aaattcttac tctcaattct tgagaatggc
     4141 ttattccatt tatctcctgc aagagtagta gatacgaggt tactaattat ttaagggcgg
     4201 ttggaaaatg aattcatcga atgtgaaaag ggatattttc ggccgcatag ccctggtatg
     4261 cagcgcaacc acagccgttc ttggggtact gggaatgatc ggttggctga gtggttttcg
     4321 ggatgttacc ggcttcggtt catattatag ttatagatca ccattcatgg ccctggagtt
     4381 tgtccttttg ggatgtgtgt ggtttttcta tgttttcaat ccaagtcaaa gattcagaaa
     4441 ggtgtttgca ctggttttag cgtttcttat cttgctcata gcactattta acctcattca
     4501 atttttcaac agcgctcaaa taggcattga aaataagctt ctggtgctgg aagtagatag
     4561 tgccacttta atgtctccca tttcagatgc cttttttctt cttgattgct ctgcaatgat
     4621 tctgttgttg ctggggcacc cgcgtcgtgt ggcaggatta tcgggaggcc tctcatttat
     4681 cgtgatttca gtgaatttag taattttact gggatatttg tatggagctc cactactcta
     4741 cggaggagag ataagaccgg ttgagcttcc tgttggagtg gcatttatcg ttctgagcac
     4801 gggactcatt gccacatccg gtgaagatta ctccctcttg aaattgatat tgggtccgtc
     4861 agtacgtgca cttttactgc gttattttct tcttgtaata cttattgttg ccctgacaga
     4921 tggtgtcttt tataacatac tatcacaatt taaactaaaa ataacaattt ccgaacttca
     4981 aggtctttca gcgttaattt atgtcatagc tgttagcgca gttgttttcc agctatcgca
     5041 tatcatcggg aacgctttat atcgttcaga gaccgcccgc aagcaggctg aagacttaaa
     5101 tgaactactt gcgcgtgaaa tctccaatcg cgaaagagcg gaggatgaac tgaagcagcg
     5161 ggcaatagaa ctcgcccgtt ccaatgcaga acttgaacaa tacgcctata ttgcttctca
     5221 cgaccttcag gaaccgctgc gcatgataac gagcttcacg cagcttctgg aaaagcgcta
     5281 caaaggcagg ctggataatg acgccgatga gttcataggt catatcattg atggggcaac
     5341 ccggatgcag atgatgatta atggtattct ggcatactct cgggcaggtt tgtcagttaa
     5401 acccttcgaa ctgacagact gcgagactgt gatcaaccag gcattggata atcttaagat
     5461 agctatcaaa gaaagtggcg ctattgtgat ccatgaccgc ctgccttccg tgatggctga
     5521 ttcttcacaa atgactacac ttatccagaa cctgctaact aacgctatca aattccgcaa
     5581 agaaactccc cgtatccatg tctgtgccaa acagaaaaga ggcgaatgga ttttttctgt
     5641 gcaagacaat ggtataggca tatctcccga attcatcgat cgtatatttg tattattcta
     5701 tcgcctgcat agcagggacg cttaccctgg ctcaggaatc gggcttgcaa cctgcaagaa
     5761 aatcgttgaa cgccatggtg ggcgcatctg ggttgaatct aagttgggaa aaggttcgac
     5821 attctatttc acaataccaa caaagagagg tgaataaaaa tatggaaaaa atgaaaaaac
     5881 gtgttgagat tttattagtg gaggataatc ccggggatat ccgcctgaca gttgaggctc
     5941 tgaaagaaaa tacgatgcac aataaactga acgtagtaaa tgatggagcg gaagcactta
     6001 ccttcctata taaaaggagc aaatacactg atgcacccag gcctgatctt atcttgctgg
     6061 atttgaacct gccgaagaag aatggtctgg aagtgctgga agagatcaaa aaagatcctt
     6121 cgctgaaaag aatccctgta gtgatattta ccgcatcaag tgcccaacgg gacatactca
     6181 acagctataa ccaccacgct aactgctaca tcaccaaacc ggttcatctg gaacagttca
     6241 tagcagtggt aaagtctata gaaaacttct ggcttactgt cgtgaaactt ccgcagggtg
     6301 attaaaatgg aaaaaatcaa agtcctgtta atagaagata atccggggga tgccaggttg
     6361 attaaggaaa tgctgactga ggccaggagc gtttcgtttg atctggaatg gaaagataat
     6421 ctctcaaaag gattgaaact cttaactgag aaagggattg atgtggtttt gcttgatctt
     6481 atgctacctg acagcgccgg ggctgaaact tttgccaaaa cacatgcctg tgcaccacat
     6541 gcaccgatat tggtgctgac aggtcttgat gatgaaacag ttgcaatcgg agctgtaaag
     6601 gagggagcgc aggattatct tatcaaaggg catatcgata gcaatctgct catacatgcc
     6661 gttcgctatg ctattgagcg catgcggatt aaagagcaac tgcaggagtc tgaaatgcgc
     6721 tatcgcactc tttttgagca atctccggat ggtgtattga tcataaaccc tctgactgca
     6781 agcgccatcg aattcaatga agccgcacac cgccagcttg gctacacgcg ggaagagttc
     6841 aagaactttc ggattttcga ttatgaagca aaggaaaaat ttgacgaaac caaatatcat
     6901 atcgaaaaat taattcgcga tggaaaggat gactttgaga cccttcaccg caccaaggac
     6961 ggcaaattca ggaatgttat ggtgactgtt caaaaaatcg agtta
//
LOCUS       DATGBN010000271         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_486964, whole genome shotgun sequence.
ACCESSION   DATGBN010000271 DATGBN010000000
VERSION     DATGBN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486964"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VKL21_06600"
     CDS             <1..731
                     /locus_tag="VKL21_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HMB45474.1"
                     /translation="SISIDGANKETHDVFRGEPGAFEGALRAIDILKKEGLSFQINTT
                     ITKRNLAEIPRIYDLAIELKASALHIFLLVPTGRGEEIESEEIPPDEYERVLNWFYDR
                     SKDKRIQLKATCAPHYFRIMRQRAKAEGIRITPQTHGMEAMTKGCLGGSAFCFVSSKG
                     DVYPCGYLPALAGNIKQKPFKMIWEKSKVFNDLRDPGMLKGKCGSCEYKNVCGGCRAR
                     AYAGTGDYLEEEPYCIHVPTSRGK"
     gene            736..1203
                     /gene="ahbA"
                     /locus_tag="VKL21_06605"
     CDS             736..1203
                     /gene="ahbA"
                     /locus_tag="VKL21_06605"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit alpha"
                     /protein_id="HMB45475.1"
                     /translation="MTLSKIFLDDIDKKILNSIQIDFPLVHKPFEMLGKSLGLEEKEV
                     INRIKRLQEEGAIRRIGPIISTKKTGGMSTLIAMKVPSGRVDEVALIINGYEEVSHNY
                     LRPSNFNIWFTVSAESNERLDEILKEITMKTNCELRNLPTKRLFKIGVKFNIK"
     gene            1246..>1552
                     /locus_tag="VKL21_06610"
     CDS             1246..>1552
                     /locus_tag="VKL21_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of
                     tetrahydropteroyl-L-glutamate and methionine from
                     L-homocysteine and
                     5-methyltetrahydropteroyltri-L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methonine synthase"
                     /protein_id="HMB45476.1"
                     /translation="MILFDDIGSFPLPKGINREWVEQAKKNNDPKLPTILKEAMQKKI
                     DAGVDVPTYPQFRDMNRMFLDIINDESSQEEPLVVRRDRARIMELSAIEDIGAQYFNK
                     "
BASE COUNT          513 a          298 c          386 g          355 t
ORIGIN      
        1 tcagcattag cattgacggg gcgaataagg aaacgcatga tgttttcagg ggagagcccg
       61 gcgcatttga aggagcgctt cgcgcaattg atatcctgaa aaaagaaggt ttaagtttcc
      121 agataaatac cactatcaca aagagaaatc ttgcagaaat cccgcgaata tacgatcttg
      181 ccattgaact caaagcaagc gctctccata ttttccttct tgtcccaaca ggaagaggtg
      241 aggaaataga atccgaagaa atcccgcctg atgaatatga acgggtgctc aactggttct
      301 atgaccggag caaagataag agaatacagt taaaagccac atgcgcccca cattatttca
      361 ggataatgag acagcgcgca aaggcagaag ggataaggat aacaccccaa acccatggca
      421 tggaagccat gacaaaagga tgccttggtg gttcagcatt ttgttttgtt tcaagtaaag
      481 gtgatgtata tccctgcgga tatctccccg cgcttgcagg taatattaag cagaaacctt
      541 tcaagatgat atgggagaaa tcaaaggtat ttaacgacct tcgcgacccg ggaatgctaa
      601 aaggcaaatg cggttcatgc gaatataaaa atgtatgcgg cgggtgccgg gcccgcgcgt
      661 atgcaggcac aggagattac ctggaggagg agccgtattg catacatgtg cccacatcaa
      721 gagggaagtg agattgtgac tttatccaaa atttttctag acgatatcga caaaaagata
      781 ttgaattcga tccagatcga ttttcctctt gttcacaaac cttttgaaat gttggggaaa
      841 agcctgggcc tggaagagaa agaagtgata aatcgaatta agaggctcca ggaggaaggc
      901 gcaataaggc gcatcggccc cataataagt acgaagaaaa cgggtggcat gagcacactc
      961 atagcaatga aggtgccttc agggagagtt gatgaagttg ccttgatcat caatggatat
     1021 gaggaagttt cgcataacta tcttcggcct tccaatttta acatatggtt cacggtttcg
     1081 gcagagagca atgagaggct ggatgagatc ctgaaagaaa ttaccatgaa gacaaattgc
     1141 gagctaagga atctgcctac gaaaaggttg tttaagatcg gtgtgaagtt caatataaaa
     1201 taggggttca ataactataa actgatagaa agaaatcagt agtctatgat actttttgat
     1261 gatataggaa gcttcccgct gccaaaaggc ataaacaggg aatgggtaga gcaggcaaag
     1321 aaaaacaatg acccgaaatt gccgactatt ctgaaggaag caatgcagaa gaaaatcgat
     1381 gccggcgtgg atgttcccac atatccccag ttccgggaca tgaaccggat gttccttgat
     1441 atcatcaatg atgaatcatc acaggaagaa cccctcgttg tccggaggga cagggcaagg
     1501 ataatggaac tttcagcaat agaagatatt ggcgcacaat attttaataa ga
//
LOCUS       DATGBN010000272         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_20693, whole genome shotgun sequence.
ACCESSION   DATGBN010000272 DATGBN010000000
VERSION     DATGBN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20693"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VKL21_06615"
     CDS             <1..233
                     /locus_tag="VKL21_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HMB45477.1"
                     /translation="FYRKLAPGQKPQTTSMDILVGKGGVVVKKVYPDSISGKVNIDNQ
                     IWSATGESEIEEGKKVLVISSEGVHIKVKEEI"
     gene            235..1335
                     /locus_tag="VKL21_06620"
     CDS             235..1335
                     /locus_tag="VKL21_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMB45478.1"
                     /translation="MAIEMFIAIFIAVAILILFISGVAIIQPYEQALWIVLGQYRKRL
                     NPGFNWVYPLISDVIRLDLRTQVLDVPRQEVITKDNSPTNVDAIVYIRVIDPGKAYFE
                     VVNYHVAVLSLAQTTLRSVVGDMELDEILYNRDFINTKLRDILDHSTDAWGVKVEAVE
                     IREVDPVGTVKGAMEEQTSAERKRRAAILIADGQKKAAILEAEGAKQSKILNAEGARQ
                     SKILEAEGDRVSQILRAQGEAQGLRILSVGAVPLDKKALTVLTMDMMKSVANGQATKI
                     IFPMEISRMIEQAAKYLGASEKIQEIGSPMDTEKIVGTADEVLGRIPRPDELRQELES
                     IEKEMARETEESLKQAEKIKSSQVPELPKKKI"
     gene            1349..1777
                     /locus_tag="VKL21_06625"
     CDS             1349..1777
                     /locus_tag="VKL21_06625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMB45479.1"
                     /translation="MDIEIRKATHADLKDLKTILSYYYLDTENVEENLSEFIIAQMNK
                     KIVGCACLDLGDVMELRSIAVLPSYRNKGVGSKLVDAILRRAHELTDIVYLRTTSPVF
                     FEKKGFKRLQNSEKKVVWKDCMQCDKYDICRQTMMKIEMT"
     gene            2131..>3050
                     /locus_tag="VKL21_06630"
     CDS             2131..>3050
                     /locus_tag="VKL21_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45480.1"
                     /translation="MVDKHASQRKYTSLGLLIMTLAALIVVPGALAVSNFPSVFNQKY
                     DTADTRIDASSCTMCHAGAPVLNDYGKALNKAGVTPSSNDTKLINGLTAIEQNDSDGD
                     GFSNIDEINARFFPGNKSDHPATTPNATATPNVTATPKVTVTPKVTVTPNVTATPNVT
                     VTPKVTATPTAKKIKEISVNDQIIIIFEDVYNYFMKLSLPKSLDNSYQNSKPGTPTSE
                     WVGEMFIMAGAFEGMVTNIQDGDMANATVSHKVFANEYNNISKKVPSWKGYFNTSAVK
                     KLGKDINMSANESTLSKDTAKVVATCEKCMG"
BASE COUNT         1062 a          518 c          713 g          757 t
ORIGIN      
        1 tcttttatcg gaagttagcc ccgggacaga aacctcaaac aacaagtatg gatattctcg
       61 ttggcaaggg aggggtggtt gtgaaaaaag tatatcccga ttcaatttca ggaaaagtca
      121 atatcgataa ccagatatgg agcgccacag gggaaagcga aatagaagaa ggaaagaaag
      181 ttttggtcat aagctcagaa ggagttcaca taaaagtaaa agaggagata taagatggca
      241 attgaaatgt tcatagcgat atttatcgca gtagcaatat tgatcctgtt tatttcaggg
      301 gtagcgatca tccagcctta tgaacaggct ttatggattg ttctcggaca gtacaggaaa
      361 cgactgaatc ctggttttaa ctgggtgtat ccactgataa gcgatgtcat aaggcttgac
      421 ctcaggaccc aggtccttga tgtcccgcgc caggaagtaa ttaccaaaga taactctccc
      481 acaaacgttg acgctattgt ttatatcagg gtcatcgatc cgggaaaagc gtattttgaa
      541 gtggttaatt atcacgtcgc tgtattatcc cttgcgcaga ctacacttcg aagcgtagtt
      601 ggcgacatgg aacttgatga gatcctttat aatcgtgatt ttataaatac aaaactgcgc
      661 gacatccttg accattccac tgacgcctgg ggtgtaaaag tcgaagccgt tgagatacgg
      721 gaagttgatc ctgttggaac agtaaaaggg gcaatggaag aacagacctc agcagaaagg
      781 aagcgaagag ctgcaatatt gatcgcggat ggtcagaaga aagctgcgat ccttgaggca
      841 gaaggcgcca agcagtcaaa gatattgaat gcagaaggcg cgaggcagtc aaagatcctc
      901 gaggcagaag gtgacagggt ctcccagata ttaagggcgc agggcgaagc ccagggactc
      961 aggatccttt cagttggcgc agtgcctctt gataagaaag cgctgaccgt tctgactatg
     1021 gacatgatga aaagtgtggc aaacggccag gcaaccaaga taatattccc gatggagata
     1081 tcaaggatga tagaacaggc tgcgaagtat ctcggagctt ccgaaaagat acaggaaata
     1141 ggttcgccca tggacacgga gaagatcgtt ggaacagcgg atgaagtcct tggaagaatt
     1201 ccaaggccgg atgaactcag gcaagaactt gaaagcattg agaaagagat ggccagggag
     1261 actgaagaga gcctgaaaca ggccgagaaa ataaaaagtt cgcaagtgcc ggagcttcca
     1321 aagaagaaaa tatgagaatc aaaactgaat ggatattgaa atcagaaagg cgacacatgc
     1381 ggatttgaag gatttaaaaa cgatcctctc ttattattat cttgatacgg aaaatgtaga
     1441 ggaaaacctg tctgaattca taattgcgca aatgaataaa aagatcgttg gttgcgcctg
     1501 tcttgacctg ggggatgtga tggaattgcg ttcaatagca gtgcttccat cctatcgcaa
     1561 taagggcgtg ggttcaaagc ttgtggacgc tattttaaga cgtgcgcatg aattgactga
     1621 tattgtttat cttcgcacca cgtcaccagt tttctttgag aagaaaggat ttaagaggct
     1681 tcaaaatagc gagaaaaaag ttgtctggaa agattgcatg caatgcgata agtatgatat
     1741 ttgcaggcaa acaatgatga aaattgaaat gacgtaagca gattttccag ataactgtct
     1801 gaacaattgt atatgagcat gaatagttat atatattttt tgttgatatt acattataga
     1861 aaaagggttt tgataattca tagaaactgt gggaggtgga atgatattat taaaagccga
     1921 tattcacgga aaataaattg ccaggaataa tgtgatgtgg caaataaatg ggaggttaaa
     1981 aagtagatga attggggatt ttttattttt tctaaaggtt gggtgggaga caactcaacc
     2041 ggaatttaag taaaaataaa cacataaaat gtgtgcagtg aaataaaccg aggtaaatat
     2101 atgaaacaaa aatatagacg aggtatagat atggtagata aacatgcaag ccaaaggaaa
     2161 tatacaagtt tgggattact aataatgaca ttagcggctc ttattgtagt gcccggagca
     2221 ttagcagtaa gtaatttccc atcggtcttc aatcaaaaat atgataccgc agacacgaga
     2281 attgacgcca gttcatgcac catgtgccat gcaggtgcgc ctgtattgaa tgattatgga
     2341 aaagctctaa ataaagcagg tgttacacca agttctaatg acaccaaatt aataaacggt
     2401 ttgacagcta ttgaacaaaa tgattccgat ggagatggct ttagcaatat tgatgaaatc
     2461 aatgctagat ttttccctgg caacaaatca gaccatccgg caacaacacc aaatgctaca
     2521 gcgacaccaa atgttacagc aacaccaaaa gttacagtaa caccaaaagt tacagtaaca
     2581 ccaaatgtta cagcaacacc aaatgttaca gtaacaccta aagttacagc aacacctact
     2641 gctaaaaaaa ttaaagaaat atcagtaaat gaccagatta ttataatatt cgaggatgtt
     2701 tataattact ttatgaaact gtctcttcct aagtctttgg ataattctta tcaaaactcg
     2761 aagcctggaa ctccaacaag tgaatgggtt ggagaaatgt tcatcatggc aggagcattt
     2821 gaaggaatgg taactaacat acaagatggt gacatggcca atgcaaccgt atcgcataag
     2881 gtatttgcta atgaatataa taatatctca aagaaagtac cgtcatggaa gggatatttc
     2941 aacacttctg cggtgaaaaa acttggaaaa gatataaata tgagtgctaa tgagtctaca
     3001 ctatctaaag atacggctaa agttgtagct acttgtgaaa aatgcatggg
//
LOCUS       DATGBN010000273         5699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_487173, whole genome shotgun sequence.
ACCESSION   DATGBN010000273 DATGBN010000000
VERSION     DATGBN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487173"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VKL21_06635"
     CDS             <1..612
                     /locus_tag="VKL21_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HMB45481.1"
                     /translation="VIDLPGHEKIKIRSFDLRQAIEGRRSIRKYLQEPLTIEELSYLL
                     WVTQGVVQVTHGATFRNVPSAGARHALETYLLINNVNDVPNGIYKFLAVGHKLVEINL
                     QPGIADRVAEGCLGQDFILKSAVTFIWTADTYRMKWRYGDRGYRYLHLDAGHVCQNLY
                     LGAGSLDCGVCAIAAFSDDHMNDLLELDGVEQFVIYIATVGKK"
     gene            679..1464
                     /locus_tag="VKL21_06640"
     CDS             679..1464
                     /locus_tag="VKL21_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45482.1"
                     /translation="MDVAAGIPDAVERSRKYKNIVGLLSQEAVRSNDQQHIEEALKIA
                     GMVTDDPSKAYVEIIRAIAGMKRKDKNRFDEAVKISESIDNNLDLSVALHEIVVGFGR
                     YSIENKDEIIYADSFDLAQKIPLNTYRAMAYRNLSKVMAGIDPKKSLDLLDISIEILE
                     NSIGIKTIYQILALCDTGSILARLNDNRSYGFLKKAIAMADSIMDDLEKSAVSLKILE
                     TEIEIGIKLKDKKLFDEAAVISKGIKKEYYKTLASNALKRYKM"
     gene            complement(1452..3080)
                     /locus_tag="VKL21_06645"
     CDS             complement(1452..3080)
                     /locus_tag="VKL21_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45483.1"
                     /translation="MRVLIIDGYVDEPACLGVPPYMSPYPRYIAGALIEKGLQKEDIT
                     YRTIDCIRSHREPLRFDLIVIIAGMTVPGKYLRSAPITLKEIRELSHLEGTKIIGGPI
                     RLGYGEEGGSSAKDLQIPGITLAKKDIEAFVYDLMEHKDPSCVQHRMRTTTEIGRWAN
                     KGAFIIIQHPDYPYLMCELETYRGCPRHSHCSFCTEPFYGKPDFREIKDVEREVSALY
                     ENGARYFRLGRQPDLFMYRSKKGVPDPGAIEELYRSIRKVAPDLKVLHMDNANPVTLS
                     KYPDESRHIAEIIVKYHTSGDVAALGMESADPDVINANDLKATPDEVFEAIKLLNKAG
                     AQRGASGLPELLPGINFVHGLKGETKKTFELNFEFLKKVLNNGLMVRRVNIRQVMAFA
                     GTPMQGHDETVRKNKEMFLRYKEKVRNEFDLPMLCRLIPAGTILKDVRTEIFDKITFA
                     RQLGTYPLLVGIPLKLDHGLSFDILVTDHGRRSITGIPVPIDINRLPIKLLNTLPGMD
                     NKQVAEVMRQRPFKDKKDIMNRTSIRGKILDHIL"
     gene            3282..3713
                     /locus_tag="VKL21_06650"
     CDS             3282..3713
                     /locus_tag="VKL21_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMB45484.1"
                     /translation="MTSKPLLYTQKWLLSFQKEIAGALFLSHDTGKVICEYRKRYGMS
                     QEEIGELMDLRRESISRIETGSVTPTFDFAKKFINAMSLIEAIRVERAQHKEIDAYFL
                     DNLAKELGFDRDKLTYMLKVAVESYDKKLVKIQKSLKEIKI"
     gene            3710..>5699
                     /gene="ppsA"
                     /locus_tag="VKL21_06655"
     CDS             3710..>5699
                     /gene="ppsA"
                     /locus_tag="VKL21_06655"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HMB45485.1"
                     /translation="MTEDSVVWLEEVGKEDIAIVGGKGASLGEMLRAELPVPTGFAVT
                     AQAFRRFIDENSINDELFGSLEIDVDNADILRNAEKKAKKIIMDAKMPEDIDNSIRLK
                     YRDLCKKEGGEVYVAVRSSATAEDLPDASFAGQQDTFLNMKGEQNVIDAVKKCWASLY
                     GARAIYYRVKQGFDHSKVNLCAVVQMMVEAEKAGVMFSSHPSTGEAQTIIEGAWGLGE
                     TVVSGSVSPDYYMVDRNSRSIKERRIATKNIMHTKDPKTGKTLDIPVPSNKKNARVLD
                     DDEILKLVEFGELLEDLYGIPQDIEWAIKNKEIFILQSRPITTIKKKEPKEKVATTAI
                     LEGLGASPGIAYGEAKLVVDASELGKVKDGDILVAVMTTPDMVPAMKRAAAIVTDEGG
                     LTCHAAIVSRELGCPAVVGTRKATELLTDGMKITVDGEKGLVFEGRKEIAAPAAQTEK
                     SAVSFTKSKPITATEVKVNVSIPEATARAIETQADGVGLLRIEHMILGLPKHPKQYIK
                     EGKADEYVAELVSRIQTVVDAFYPRPVWVRTLDAPTDELRAMEGGEGEPVESNPMLGF
                     RGIRRDLIDTEHFELEVRAFKELIKRGYNNMGIMIPLVQHPTELRRAKEFMRKNGLDI
                     DKIDIGIMVEIPAAALIIDQFLAEGLNFVSFGTNDLTQY"
BASE COUNT         1722 a         1117 c         1345 g         1515 t
ORIGIN      
        1 gtaatagatc ttccaggtca tgaaaaaatc aaaataagaa gttttgactt gaggcaggcc
       61 attgaaggtc gaagaagtat acgcaagtat ttacaggaac cattaacaat tgaagaactg
      121 tcatatttat tgtgggttac acagggagtg gttcaggtca cccatggtgc aactttcagg
      181 aatgtgcctt ctgcaggtgc aaggcacgct cttgaaacgt atctgctcat caataatgtc
      241 aatgatgttc caaatggtat ttacaagttt ttggctgtcg gtcacaaact ggtggaaata
      301 aacttgcagc ccggcatagc tgatagggtc gctgaaggct gtttaggaca ggactttatc
      361 ctgaaaagcg ctgttacttt tatctggaca gcagatactt acagaatgaa atggcggtat
      421 ggtgacagag gatacaggta tctccacctt gatgcaggac atgtctgcca gaacctgtac
      481 ctgggcgcag gctctttaga ttgcggtgta tgcgccattg cagctttttc agatgatcat
      541 atgaacgatc tgctggagct tgatggagtg gaacaatttg taatatatat tgcaactgtg
      601 gggaaaaaat aaataacaaa agagcctata aattcttgaa tattacaagg tgataatata
      661 accagaataa aagagctgat ggatgtcgct gccggtattc ctgacgcggt tgaacgttca
      721 cgaaaataca aaaatattgt ggggctgcta tcacaggaag cggtgcgttc aaacgatcaa
      781 cagcatattg aagaagcatt gaaaatagcg ggaatggtga ctgatgaccc atccaaagct
      841 tatgttgaga tcatccgcgc gattgcagga atgaaacgaa aagataaaaa taggttcgat
      901 gaagctgtaa aaatttcgga gagtatcgat aacaaccttg atctttccgt ggctctgcat
      961 gaaatcgtgg ttgggtttgg aagatacagc attgaaaaca aagatgaaat tatctatgca
     1021 gactcatttg accttgcgca aaagatacca ttgaatacat atcgtgcgat ggcttaccgg
     1081 aatctttcaa aagtaatggc aggtattgat ccgaaaaaat cactggattt gctcgatata
     1141 tctatcgaga tactcgaaaa tagcataggt atcaaaacta tatatcagat cctggcattg
     1201 tgcgatacag gttcgattct tgccaggctt aacgataata gaagttatgg gttcttaaag
     1261 aaagctatag caatggctga tagcattatg gatgatcttg aaaaatcggc agtttcactt
     1321 aagattttgg aaactgaaat tgaaatcggg ataaaattaa aagataaaaa actatttgat
     1381 gaagccgcag tgatctcaaa agggatcaaa aaagaatatt ataaaaccct ggcttcaaat
     1441 gctttaaaac gttataaaat gtgatccagt atttttcccc ggatcgaagt ccgattcata
     1501 atatcctttt tgtctttgaa aggtctttga cgcatcactt cagcaacctg tttattatcc
     1561 attcccggaa gcgtattcag taatttaatt ggaagtcggt taatgtcaat tgggacaggg
     1621 atcccggtta tcgacctgcg cccgtgatct gtcaccagta tatcaaagga gaggccatga
     1681 tcaagtttca ggggaatccc gacaagtaaa ggataggtgc caagctgccg tgcgaatgtg
     1741 atcttatcaa aaatctcagt cctgacatct ttaagtatcg tcccggccgg gatcaaacgg
     1801 catagcattg gaagatcgaa ctcattgcgt accttttctt tatatctgag gaacatttcc
     1861 ttgttctttc ttacggtttc atcatgcccc tgcatcggag tgcccgcaaa ggccatcacc
     1921 tgcctgatat tcactctgcg aaccatgagg ccattattta atactttttt caggaattca
     1981 aagttaagtt caaatgtctt ttttgtttca ccttttagac catgaacaaa atttattcct
     2041 ggcaatagtt caggaagacc gcttgctcca cgctgcgctc ctgctttgtt caagagtttt
     2101 atggcttcaa atacttcatc gggagttgcc ttcaggtcat ttgcatttat cacatcaggg
     2161 tctgcgcttt ccatccccag tgcagcgaca tctcccgatg tatgatattt cacgattatt
     2221 tctgcaatat gccttgattc atcgggatac tttgacagcg tcacaggatt ggcattatcc
     2281 atatgaagca ccttaaggtc tggcgcaact ttccttatgc tcctgtatag ttcttcaata
     2341 gctcccgggt caggaacacc tttttttgac cggtacataa atagatcagg ttgtcttcct
     2401 agcctgaaat accgggcgcc attctcatag agagccgata cttctctttc aacatcctta
     2461 atttcacgaa aatccggctt cccataaaaa ggttcggtac agaaagaaca gtgtgaatga
     2521 cgcggacatc cccgataggt ctcaagttcg cacatcaaat acggataatc aggatgctgg
     2581 attataatga aagcgccttt atttgcccat cttcctatct ctgtggtggt ccgcatcctg
     2641 tgttggacgc aagaggggtc tttgtgttcc ataagatcat aaacgaatgc ctcgatgtct
     2701 ttctttgcaa gcgttattcc agggatttgg aggtcttttg cagatgaacc gccttcctcg
     2761 ccatatccaa gcctgatagg gcccccgatt atctttgtac cctcaaggtg tgacagttct
     2821 cttatctctt tcagggtgat aggcgctgac cttagatatt tcccgggaac tgtcattccc
     2881 gctatgatga caataagatc aaatcgcaga ggctctctgt gcgaacggat gcagtctatg
     2941 gttcgatagg ttatatcctc cttttgcaat cctttttcaa tgagagcgcc tgctatatac
     3001 cgcggataag gagacatata cggtggaacg ccaaggcatg cgggttcatc cacatacccg
     3061 tcaattataa gaacgcgcat atgttatcat tatctgcttc atattttggc ctttcgttta
     3121 tttttacgat attccataaa aagaaatgca aataatgtgg catgataatc atgttatttt
     3181 tcattgtttg ttgaaaaagt attaatatca gcaattcaaa atagcaaaca aaattgaatg
     3241 tgacatttaa gttaacatcg attcaggaga aatactttaa attgacatca aaacccttgc
     3301 tctatactca gaaatggctt ttatcatttc aaaaagaaat agctggagca ttatttttat
     3361 ctcacgatac cgggaaagta atttgtgaat acaggaagag atatggcatg tcacaggaag
     3421 aaatcgggga gttgatggat cttcgccgtg aatccatctc gcgcattgaa acaggaagcg
     3481 tgactccaac atttgatttt gcaaagaaat tcattaacgc tatgtcacta atcgaagcga
     3541 tccgggtgga gagggcgcag cataaggaaa tcgatgcgta tttccttgat aatctggcaa
     3601 aagaactggg gtttgacagg gataaactca catacatgct caaagtggcg gtagaaagtt
     3661 atgataaaaa actcgtaaag attcaaaaat cattaaagga gataaaaata tgacagaaga
     3721 ttcggttgta tggcttgaag aggttggaaa agaagatatc gcgatcgttg gtggaaaagg
     3781 agcaagtctt ggtgagatgt tgcgggcaga actgcctgta cctacgggtt ttgcggtgac
     3841 cgcacaggct ttccggagat ttattgatga aaatagtata aatgatgagc ttttcggctc
     3901 acttgaaatt gatgttgata atgcggatat cttgaggaat gcggagaaaa aagcaaaaaa
     3961 gataataatg gatgcaaaaa tgcctgaaga tatcgataac agcatcaggt taaaatatcg
     4021 ggatctgtgt aagaaagagg gaggagaggt ttatgtggcg gtccgctcaa gtgcgaccgc
     4081 cgaagatctg ccggacgcca gtttcgcagg acaacaggat acatttctta atatgaaagg
     4141 agaacaaaac gtgatcgatg ctgtgaagaa atgctgggca tctctttatg gggcgagggc
     4201 catctattac cgcgtgaagc agggttttga tcacagcaag gtaaatcttt gcgcggttgt
     4261 ccagatgatg gtagaagctg aaaaagcagg cgtcatgttc tcgtcacatc cttcaaccgg
     4321 agaggcgcaa actataattg aaggcgcatg gggattgggg gagactgtgg tatcaggctc
     4381 ggtttcaccg gattattata tggtagatag aaattccagg agtataaaag aacgaagaat
     4441 tgccaccaag aatatcatgc atacaaagga tccaaaaaca gggaaaaccc tcgatattcc
     4501 ggtgccatcc aataagaaaa atgccagggt tctggatgat gatgaaatat tgaaactcgt
     4561 tgagttcggg gaattgctgg aagacctgta tggtataccc caggatatcg aatgggcgat
     4621 aaaaaacaaa gagatattca tcctccaatc aagacccatc acaactatca aaaagaaaga
     4681 acctaaagaa aaagtcgcaa ctaccgccat tcttgagggg ctgggagctt caccgggtat
     4741 tgcatacggt gaggcaaagc tggtcgtgga tgcaagcgaa cttggtaaag tcaaagacgg
     4801 tgatattctt gtagcggtca tgacaactcc ggatatggtc cctgcaatga aacgggctgc
     4861 agctatcgta actgatgaag gcgggcttac atgccatgca gccatcgtgt caagagaact
     4921 tggctgtccg gctgttgtgg ggacaaggaa ggcaacagag ttattgacag atggcatgaa
     4981 aataacagta gatggtgaaa agggacttgt atttgagggc aggaaagaaa ttgcagcccc
     5041 tgccgcacaa acggaaaaat cagccgtatc atttacaaaa tcaaaaccta taacagcaac
     5101 cgaagtgaaa gtgaacgttt ccattcccga agcaacagcc cgggctattg aaacacaggc
     5161 ggacggtgtg ggccttttgc gtatcgagca catgatcctt ggtcttccca agcatcccaa
     5221 acagtacata aaagaaggaa aggcggatga gtatgttgca gagcttgttt cgcggatcca
     5281 gaccgttgtt gatgctttct atcccaggcc agtatgggta agaacacttg atgcccccac
     5341 ggatgagtta agggcgatgg aaggcggcga gggcgaaccc gttgagtcca atccgatgct
     5401 tggattccgc ggtatcaggc gcgacctcat agacactgaa cactttgaac ttgaagtgag
     5461 ggcattcaag gaattgataa agcgcggtta caataatatg ggtataatga tccctcttgt
     5521 ccagcatcca acggagctga ggcgggcaaa agagttcatg cgaaagaacg ggcttgacat
     5581 tgataagatc gatatcggga taatggttga gataccagcg gcagccctta ttatcgacca
     5641 gttcctcgcc gaagggctga atttcgtgag cttcggcacg aacgacctca cgcagtaca
//
LOCUS       DATGBN010000274         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_332422, whole genome shotgun sequence.
ACCESSION   DATGBN010000274 DATGBN010000000
VERSION     DATGBN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332422"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VKL21_06660"
     CDS             <1..204
                     /locus_tag="VKL21_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HMB45486.1"
                     /translation="NGKQEKFHDFYKNHRGRFESLDKMIEWYNNRPHGSLNLRRAETP
                     NQAFIRKMHPEVWLGFAFKMFGW"
     gene            272..412
                     /locus_tag="VKL21_06665"
                     /pseudo
     CDS             272..412
                     /locus_tag="VKL21_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917481.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            399..1613
                     /locus_tag="VKL21_06670"
     CDS             399..1613
                     /locus_tag="VKL21_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45487.1"
                     /translation="MNLIRTEQIQVKGTKELSALCHLSKNLYNQANYVVKQEFITTGN
                     WIKGFDLIGKCKGFENYKLLNIQSAQQTIRLVDKNWTSYFASIKEWKKHHDKFLGIPK
                     MPHYKPKDGEAILVFTNQQAHIRNHNLTFTKKLPLKIKTRLSGKINQVRFIPKGTGYT
                     CEVIYEKQSEEGEINKRLYAKRVNSKRIIGIDLGLRNVVTIANNIGEKPIVIKGGILK
                     STNQFYNKEKAQLQSIYDKQGIKFGTRMSQLNSKRNNKIKDTMHKISRYTIDYCLENN
                     IGTIVIGKNDNWKQETNMGKKNNQNFVQIPHFKLIQMLQYKAEAEGITVKLQQESHTS
                     KCSFLDSEPVCHQDTYKGVRFTRGLFRSANGTILNSDVNGAYNIIRKAIPDAFAKGIE
                     GVGLHPIRSNTL"
     gene            1610..2500
                     /locus_tag="VKL21_06675"
     CDS             1610..2500
                     /locus_tag="VKL21_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HMB45488.1"
                     /translation="MNGNINTVNSFHSLIHLGKIAFANCDFPYYAMEHGNLRTDDIDI
                     IEAHPVELARRLQRGKLDISPISSIMYAKADDLLILPGLSITSNDFTKSVLICSNDIE
                     MSGFEGKTLCIPETTASSATLVKIIFWLKGVNVKIRQCNGSDIEKMLQQGDAALLIGD
                     SAIHAIGKYRIIADLGNEWKKLTGKKMVYALWVARGDFARKFPEKVKYVHDLLLESKD
                     FAYKNINDIASHIGRQKNIDCNFMREYLHTLNYDFDKESVESLKLFFKYARECGIIGD
                     VKLRFFDNLILDEYKGNINE"
     gene            complement(2618..3049)
                     /locus_tag="VKL21_06680"
     CDS             complement(2618..3049)
                     /locus_tag="VKL21_06680"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMB45489.1"
                     /translation="MEQNKWLYHNTLKWKGEKKADLKFIESKQEIEVATPPEFGGHED
                     IISPEDLFVSSANVCYMTTILGTMKNMSVELVSYESRAEGILETVDKMKIFTKIIIRP
                     RIKAKDTEAKIRMILNHAEKRCLVANSMKTQVIIEPEIETA"
BASE COUNT         1112 a          501 c          590 g          930 t
ORIGIN      
        1 aatggaaaac aggagaagtt tcacgatttt tacaagaacc acaggggaag atttgaaagt
       61 ctggataaaa tgatcgagtg gtacaataat cgccctcacg gatctttgaa tttaagacgg
      121 gcagaaacgc caaaccaggc attcattcgg aagatgcatc ctgaggtctg gctcggtttt
      181 gcttttaaga tgtttggatg gtgaatttta tgaaaactgt acaaaaggta aaagaagtta
      241 agactgagga aattattcca ggaaaagata gtcagaaata gggaatacta aacttgattc
      301 cgaagaaata tttgaagaaa taaggtcttt acttcgttgt tattcgatga agctttatag
      361 taaacgaaat aatattaaac acatcgaggt ttccacagat gaacttgatt agaacagaac
      421 agattcaagt aaaaggtact aaagaattat ctgctttatg tcatttatct aagaatttgt
      481 ataatcaagc taattatgtg gtaaaacaag aattcattac cacaggaaat tggattaaag
      541 gatttgattt aattggtaaa tgtaaaggat ttgagaatta taaacttctt aatattcaat
      601 cagcacaaca aacaataaga cttgttgata aaaactggac ttcatatttt gcatcaataa
      661 aagaatggaa aaaacatcat gacaagtttt taggaatacc caaaatgcca cactataaac
      721 caaaagatgg tgaagcaatc ttagtattca caaaccaaca agcacatatt agaaatcaca
      781 acttaacatt cacaaaaaaa cttcctttaa aaattaaaac aagactttca ggaaaaataa
      841 atcaagttcg tttcataccg aaaggaacag gttatacttg tgaagtaatc tatgaaaaac
      901 aatcagaaga aggtgaaata aacaaacgct tgtatgcgaa aagagtaaat tcaaaaagaa
      961 ttataggaat tgatttagga ttaagaaatg tcgtaactat tgcaaataac attggtgaga
     1021 aaccaattgt cattaaaggc ggtattttaa agagcacgaa tcaattttat aataaggaaa
     1081 aagcacaact gcaatcgatt tatgataaac aagggattaa atttggaaca agaatgtctc
     1141 aacttaattc taagcgaaat aataagatta aagacacaat gcataagatt tccagatata
     1201 ccatagatta ttgtttggag aataatattg gaacgattgt aatcggtaaa aacgataatt
     1261 ggaaacaaga aaccaatatg ggaaagaaga ataaccaaaa ctttgtccag attccacact
     1321 ttaaattaat tcaaatgctt caatataaag cagaagcaga aggaataacg gtaaaactgc
     1381 aacaagaatc ccatacttca aagtgttcat tccttgattc agaaccagtt tgccatcaag
     1441 atacttacaa aggtgtaagg ttcacaagag gtctttttag atcagcaaat ggaacaatct
     1501 taaattcaga tgtaaatggt gcttacaaca ttataagaaa agcaattcct gatgcatttg
     1561 caaaaggaat agagggtgta gggctacacc caataaggag taatacctta tgaatgggaa
     1621 cattaatact gttaatagtt ttcatagcct gattcatctt ggtaaaattg cctttgctaa
     1681 ttgtgatttt ccctattacg caatggaaca tggaaatcta agaacagatg atattgacat
     1741 tattgaagcg catcccgttg aacttgcacg aaggttgcaa cgcggtaaac ttgatataag
     1801 ccccatatca tcgatcatgt atgcaaaagc ggatgacctt ttaatattac ctggactttc
     1861 cattacttca aatgatttta ccaagagtgt cctgatatgt tcaaatgata tcgaaatgtc
     1921 cggttttgag gggaaaaccc tatgcatccc tgagactact gcaagttcgg caacacttgt
     1981 aaaaataatt ttttggctaa agggtgttaa tgtaaaaatc aggcaatgca atggatcaga
     2041 tatcgaaaag atgctgcagc agggcgatgc tgctcttttg atcggagata gcgcaattca
     2101 tgcgattggg aaataccgta taattgccga tcttgggaat gaatggaaaa agcttacggg
     2161 caagaaaatg gtatacgcat tgtgggtcgc aagaggggat tttgccagga aatttcctga
     2221 aaaagtaaaa tatgtccatg accttctcct tgaatccaag gattttgcgt ataaaaacat
     2281 taacgatata gccagtcaca taggacggca gaaaaatatc gattgtaatt tcatgaggga
     2341 atatcttcac actttaaatt atgattttga caaagaaagt gtggaaagcc tgaagctctt
     2401 ttttaaatat gccagggaat gcgggatcat aggggatgtg aagcttcgat ttttcgataa
     2461 tttgatactt gatgaatata aaggaaatat caatgaatag ccgatggata ccttgtttta
     2521 gtggcagttt tgatttcggg tcgtgctgat aagaatacag atgccaggtg ccaaaggaaa
     2581 aaaatattag tcaaataata ggaatgcgct tcctaattca ggctgtttca atctccggct
     2641 ctattatgac ctgcgtcttc atggagttcg caacaaggca tcgcttctcg gcgtgattta
     2701 agatcatcct gattttggct tccgtatctt ttgcttttat cctgggtctt attattattt
     2761 ttgtgaatat tttcatctta tcaacagttt caagtattcc ttcggccctg gattcatagg
     2821 ataccaattc aacactcata ttcttcatcg tgccaaggat tgtcgtcata tagcaaacgt
     2881 ttgctgatga cacgaaaagg tcttcagggc tgataatatc ctcatgtccg ccgaattcag
     2941 gaggcgttgc tacttctatt tcctgtttgc tttcaatgaa tttgaggtct gcctttttct
     3001 cccctttcca tttcagtgtg ttatgatata accatttatt ctgttccatc gatatcatcc
     3061 ttttatcttt taaaataaaa catctttccg gatattaaac tgttcggatt aaatacattc
     3121 ttctatatct ata
//
LOCUS       DATGBN010000275         5125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_99384, whole genome shotgun sequence.
ACCESSION   DATGBN010000275 DATGBN010000000
VERSION     DATGBN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99384"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VKL21_06685"
     CDS             complement(<1..285)
                     /locus_tag="VKL21_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194343.1"
                     /note="catalyzes the formation of orotidine monophosphate
                     from 5-phosphoribosyl-1-pyrophosphate and orotate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMB45490.1"
                     /translation="MTSLKDSLKECEAIRFGDFTLASGKKSSYYIDIKKASAVPRILK
                     QIAKEISEKIKLRSIEADYIGCVALGGVPIAVAVSLETDLPLIIIRKEAKE"
     gene            complement(285..836)
                     /locus_tag="VKL21_06690"
     CDS             complement(285..836)
                     /locus_tag="VKL21_06690"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HMB45491.1"
                     /translation="MFEIIATITGLIIKASWLMLPAYLANPAAVVFGGGTPIDLRKNW
                     SDGRRIFGDGKTFRGLIGGTASGVATGLIQMQITSLQYLGTFTLISIFMLSFGALLGD
                     MTKSFFKRRLGYERGAKFPLVDQLDFVAGAWVLTYAFDPVWFSDNFLASPWIMVTVVL
                     LTPLLHRLTNIIGYYAGLKKEPW"
     gene            complement(842..1288)
                     /locus_tag="VKL21_06695"
     CDS             complement(842..1288)
                     /locus_tag="VKL21_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HMB45492.1"
                     /translation="MRPTLDEYFMEIATIVAKRSTCLRNQVGAVIVKDKRILSTGYNG
                     APRNLEHCLDIGCVRQQNNIASGTRHELCRAVHAEQNAIIQAALHGVSIEKATVYCTH
                     QPCILCAKMLINSRIEKVVFGTVYPDKESLDFFDKAGVRVERFTLR"
     gene            1348..2034
                     /gene="queC"
                     /locus_tag="VKL21_06700"
     CDS             1348..2034
                     /gene="queC"
                     /locus_tag="VKL21_06700"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HMB45493.1"
                     /translation="MKSIVLLSSGLDSTVAFKEAYNISDEILCVTFDYGQKAREKEIL
                     YSKRICERYKAGHIVIELPWYRTFRGALTGKSALPEISGNELDNMEIARRSAQMVWVP
                     ARNVVFLSIGAALAENYKYDTIVTGFDAEEAATFPDNTIEFVECFNEMLKFGTLTKTW
                     VWAPLISMNKYDIVRRGYDIDAPLEWSWSCYEGTEKPCGICESCLRRKRAFEITCTKD
                     PLLERLESEA"
     gene            complement(2031..2747)
                     /locus_tag="VKL21_06705"
     CDS             complement(2031..2747)
                     /locus_tag="VKL21_06705"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB45494.1"
                     /translation="MDRIKTGINELDKLIGGFPVGKTVLVTGDAGSGKTILGLQFANI
                     CCMEGKKTVYISTEECADDLRQQGKSFGWDFGSHGKKSFLKFIELAGNRAMEIETSLD
                     ISLDTTKGNFNELLDQIPGDTKILIIDSLGNRTANLTPHEFRDRFDLLVYNLNKREIT
                     ALVILDSATSREFHDLALFSVYGAIQLLKRENPYTDRRERVMDIVKMRNTRTPIQLLA
                     YEISAEGIVISSPIDSSGID"
     gene            complement(2804..3775)
                     /locus_tag="VKL21_06710"
     CDS             complement(2804..3775)
                     /locus_tag="VKL21_06710"
                     /inference="COORDINATES: protein motif:HMM:NF037269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HMB45495.1"
                     /translation="MSNKGSRIKAQRHDPEDQYRLLFESNPHPMWVYDLDTLEFLTVN
                     EAAIHRYGYSRDEFIGMTIKDIRPPEDIQALLENVSRVTEGLDDAGIWRHKKKDGSII
                     FVEIVSHTINFNGRRAEMVLANDITKRKQAEDALKLSEEKYRGANKLQREKTIAELYG
                     FIVSALPVFASNVPSQVRHNLIRNFAERFDKNIRPRFEEEIKQLPCGLSVEKSINEGV
                     PEKLDIFMLWLERMFENINIRTNISFEKTDQEGIRANLEFLNCSWEGEAKVNPIFCFI
                     CRTIVLRSFTWTKQKGSAQQKSSIANGSKTCRFEIQVKSPVDCAGII"
     gene            complement(3903..4121)
                     /locus_tag="VKL21_06715"
     CDS             complement(3903..4121)
                     /locus_tag="VKL21_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45496.1"
                     /translation="MTDGKKKLAKPASVNILECGFIPRMAFKKPKFFSSLYNYSTIFH
                     VWIGKNVITKGVDKPGTVGECLIKFELE"
     gene            complement(4333..4677)
                     /locus_tag="VKL21_06720"
     CDS             complement(4333..4677)
                     /locus_tag="VKL21_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMB45497.1"
                     /translation="MSKLTNYGPTIKIAPLLIVVFLDKDHMYNYVKDVQSIGACMQNM
                     LLAIHSMGLGGVWLGEILKNKEIVNKVLETPESFELMGVIALGYPVHKKMVSERKELD
                     QLVFREKFGKNR"
     gene            complement(4738..>5125)
                     /locus_tag="VKL21_06725"
     CDS             complement(4738..>5125)
                     /locus_tag="VKL21_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45498.1"
                     /translation="VCAVRPRMSKCMNGNESMKDMIISLSKKNRKNTFLKNIIELKCI
                     CGFSEKISYYDFLSGGEFEIGQTILTVSPYISESIYDETIRVTPLNLSRNCPSCREKI
                     AQMFPISLENLILMLQMQPPDPLMYG"
BASE COUNT         1419 a         1135 c          961 g         1610 t
ORIGIN      
        1 ttccttggct tctttcctga ttattatcag cggcaggtcc gtttccagcg atacggcgac
       61 ggctatcggg acgccgccaa gcgcgacaca tcctatgtaa tcagcttcaa tggagcgcag
      121 ctttattttt tcagaaatct ctttagctat ctgttttaag atccggggaa ctgcactggc
      181 ttttttaata tcgatataat aactgctctt ctttccggat gcaagggtaa aatccccgaa
      241 cctgatagct tcgcattctt taagggaatc tttaagtgag gtcattacca gggctccttt
      301 tttaatccgg cataatatcc tataatattg gtcagccgat gtaaaagcgg cgtgagaagc
      361 actacagtta ccattatcca gggcgatgcc agaaaattat ccgagaacca gaccgggtca
      421 aaagcgtatg tcaggaccca ggcgcctgct acaaaatcca gctggtcaac aagagggaat
      481 tttgcacccc gttcataccc tagccttctc ttaaaaaaac tttttgtcat atctccaagg
      541 agagcgccaa aagacagcat gaatatagat atcagggtga aagtcccaag atattgaagt
      601 gatgtgatct gcatttgtat gagacctgtg gcgacaccac ttgctgtacc tccgataaga
      661 ccgcggaatg tcttgccgtc accgaatatt cttctcccgt cactccagtt cttcctgaga
      721 tcaatgggtg tcccgccccc gaaaacaacc gcggccggat ttgccagata agcaggaagc
      781 atcaaccatg atgcttttat tattaatcca gtaatagttg cgattatctc aaacattagc
      841 ctcacctgag ggtaaatcgc tctaccctga cccctgcttt atcaaaaaaa tccagagatt
      901 ccttatccgg atatactgtc ccgaatacga ctttttcaat tcttgaattg atcagcatct
      961 ttgcacacag gatacacggc tggtgcgtgc aataaaccgt tgctttctct atacttacac
     1021 cgtgaagcgc agcctgtata atagcatttt gctctgcgtg aacggcacgg cacagttcat
     1081 gccttgtccc ggatgcgata ttgttttgct gcctgacgca tccgatatcc agacagtgtt
     1141 caaggttcct tggcgcgcca ttataacctg tggaaagtat cctcttgtcc ttaactatca
     1201 ctgccccgac ctggttcctc aggcatgtag agcgttttgc aactatggtt gctatttcca
     1261 tgaaatattc atccagtgtg ggtcgcatcg gcgataaata accaccaaac gataaatatt
     1321 tactgcttaa agattcaatt gtttgatatg aaatcaattg ttttactttc atcaggtctt
     1381 gattctacag ttgcattcaa agaagcatat aacataagtg atgaaatcct gtgcgttact
     1441 tttgactacg ggcagaaagc aagagaaaaa gagatcctgt attcaaagag gatatgtgag
     1501 cgatataagg cgggccacat tgtcatagag cttccatggt acaggacatt tcgaggcgct
     1561 ctgacaggaa aaagcgcttt gcctgaaata tcaggaaatg aacttgataa tatggaaatt
     1621 gcgcgaagat cagcacaaat ggtatgggtg ccggcgcgca atgttgtttt tctctcgatt
     1681 ggcgcagccc ttgccgagaa ttataaatat gatactattg tcacgggttt tgatgcggag
     1741 gaagctgcca cttttcctga taatacaata gaatttgttg aatgtttcaa tgagatgcta
     1801 aaatttggaa ccctcaccaa aacatgggtc tgggcgcctt tgatctccat gaataaatat
     1861 gatatcgtaa ggcggggcta cgatatagat gctccccttg aatggtcatg gtcatgttac
     1921 gagggcacag aaaaaccctg tggtatatgt gaatcatgtc tcaggagaaa acgcgctttt
     1981 gaaattacat gcacaaaaga cccgttgctt gaaagattgg aatccgaagc ctaatcaatt
     2041 cctgaagaat caatgggcga tgatatcaca ataccttcag cgcttatttc gtaggcaagc
     2101 agctggatag gagttctggt attcctcatt ttcactatat ccattaccct ttccctgcgg
     2161 tctgtatacg gattctccct tttcagaagt tgtattgcgc catataccga aaaaagggcc
     2221 aggtcatgga actcccttga agtcgcgctg tcaaggataa caagggctgt aatttccctt
     2281 ttattcaagt tatatacaag aagatcgaac ctgtctctga attcatgggg ggtcaggtta
     2341 gccgtgcgat tccccagact atcaatgata aggatctttg tatccccggg gatttgatcc
     2401 agaagttcat tgaaattgcc ttttgtggta tcaagactga tatccaatga tgtctcaatt
     2461 tccatagccc tgttgcctgc caattcgatg aattttagaa aacttttttt tccatgcgat
     2521 ccaaaatccc atccgaatga cttcccctgc tgcctgagat cgtctgcaca ttcttccgtt
     2581 gaaatataga cagtcttttt accttccata caacatatat ttgcaaactg cagcccaaga
     2641 attgtctttc cactacctgc atcacctgta acaagaacag ttttaccgac cggaaaacct
     2701 cctatcagct tgtccagttc gttaatacct gttttaattc tatccatggc ttattaacat
     2761 ctcttactct cttatatcat atattattat gctttcatat atattaaata attcctgcac
     2821 aatcaacagg ggatttaact tgaatttcaa atctgcaggt ctttgagcca ttcgctatgc
     2881 ttgatttctg ctgcgcgctt cctttctgtt ttgtccatgt gaaacttcgc aacactatag
     2941 tcctgcatat gaagcaaaat atcggattta cctttgcttc cccttcccag gaacagttca
     3001 aaaattccag attagctctt attccttcct gatctgtttt ttcaaatgat atatttgtcc
     3061 ggatattaat attttcgaac atcctttcca gccataacat gaaaatgtca agtttttcag
     3121 gaacaccttc attgatgctt ttctccacac ttaagccgca tggcagttgt tttatttctt
     3181 cctcaaacct gggtcttatg ttcttatcaa aacgttctgc aaagttcctt atcaggttgt
     3241 gtctcacctg tgaaggaaca ttggatgcaa aaacaggcaa tgcgctcact atgaaaccat
     3301 atagttcagc aatcgttttt tctctttgca gcttatttgc acccctgtat ttttcctctg
     3361 ataatttcag ggcgtcttct gcttgctttc tcttcgttat gtcatttgca aggaccatct
     3421 cggcgcgtct gccattgaaa tttattgtgt gtgaaacgat ttccacaaaa ataatacttc
     3481 catccttttt cttgtgcctc catatacctg catcatcaag cccttctgta actctggata
     3541 cattttcaag aagggcctgt atatcttccg gaggacggat gtcttttatc gtcatcccga
     3601 taaattcgtc cctggaatat ccatatcgat ggatagctgc ctcgttaacc gtgagaaatt
     3661 caagtgtgtc aaggtcataa acccacattg gatgagggtt gctttcaaac aatagcctgt
     3721 actgatcttc aggatcatgc ctctgtgcct taattctgct tcctttgttg ctcaaaggaa
     3781 attacccctg agaattccgg ggttgaagaa aatgtaatct atatcgttat tccgacaaat
     3841 ctccattaat atatattaat aatccaaata gttcttaaat gtttgcttcg tatatagaca
     3901 aactattcca attcaaactt gatgaggcac tcgcccacgg ttccaggttt atccacacct
     3961 tttgttatta cattttttcc gatccataca tggaaaattg tgctgtagtt gtataacgaa
     4021 gaaaaaaatt tcggtttctt aaaagccatc cttggaataa aaccgcattc caaaatgttc
     4081 acactggccg gcttcgcaag cttcttcttg ccgtctgtca caatgcgaaa gagttaagag
     4141 tcgtattgcc atatcgtgtt tttcgcagta cggtgatact atcattttat tcctcctttt
     4201 atgttaacga atctttgaat taatcgttaa caatatgtaa ataaaccaca taatatataa
     4261 tattaattgt taattatatt attctaataa ttatcattac catacaatga tgattgaaaa
     4321 aaacgatgag cattacctgt tttttccgaa tttttcccgg aacacaagtt gatccagttc
     4381 tttcctttct gaaaccattt tcttatgaac aggatacccc agggcaatta cacccattaa
     4441 ttcaaaagat tcaggagttt caaggacttt attgacaatt tctttgttct tcaggatctc
     4501 accaagccat actcctccaa gccccatgga atgaatagca aggagcatat tctgcatgca
     4561 tgcacctatc gactgtacgt cctttacata attatacata tgatccttat ccagaaaaac
     4621 aactatcagt aatggcgcga tcttgatggt ggggccataa ttggtaagct ttgacaatgc
     4681 gatttttgta tcaggatttc gtactatgat tttattgctt cgatcatatc cattatatca
     4741 cccatacata agaggatcag gaggctgcat ttgtagcatc aggatgaggt tttcaagtga
     4801 aatcgggaac atctgggcaa ttttttccct gcaagaagga caattccgtg ataaattaag
     4861 tggcgttacc cggatcgttt catcataaat ggattcggaa atgtatgggc tgactgtcag
     4921 gatggtctgc ccgatttcga attctccacc ggaaagaaaa tcataataag aaatcttctc
     4981 ggaaaatcca caaatacatt tcaattcgat tatatttttc aggaatgtat ttttcctgtt
     5041 ttttttggac aatgaaataa tcatatcttt cattgattca tttccattca tacatttcga
     5101 catgcggggt ctcacagcgc agaca
//
LOCUS       DATGBN010000276         1792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_487333, whole genome shotgun sequence.
ACCESSION   DATGBN010000276 DATGBN010000000
VERSION     DATGBN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487333"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VKL21_06730"
     CDS             complement(<1..393)
                     /locus_tag="VKL21_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase regulatory subunit PriL"
                     /protein_id="HMB45499.1"
                     /translation="MDVFKFAYFPFVNAAAKYVEALDFKLDELFSERVFEQVRERGKN
                     RVLESIGEGIIRNVSPDRVSAEKELLSYPVARILVSCLNDGYLIKRYALSEAKSAFET
                     VRELREDELKEFALDFNINAVPGEKGVII"
     gene            complement(396..1133)
                     /locus_tag="VKL21_06735"
     CDS             complement(396..1133)
                     /locus_tag="VKL21_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HMB45500.1"
                     /translation="MFKALIGAEKLKDSIESISTLVDEARFRITPEGLSVKAVDPANV
                     AMVSFELSKDAFDSFEATEGELGIDLTKLTGVMEMADKSDIIELELDEASHKLIVRMR
                     GLAYTMSLLDPSSIRKEPKVPNLDLPVHIVIRGEDIKRAVKAAEKVSDYMSMGVRGDI
                     FFMEAEGDTDKVLVELGKEQLIDLQATDAKSLFSLDYLSDMSKILGKSGEVTIDLGKD
                     FPLKIRIKIAEGHGEVSYMLAPRVESE"
     gene            1247..1732
                     /locus_tag="VKL21_06740"
     CDS             1247..1732
                     /locus_tag="VKL21_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45501.1"
                     /translation="MTGRSIDIALTQAEAGEKTETVYENVCTLSTEPELCFNYATTDD
                     LKKWGFSEGCFSCLLKDEKDTIIQVITTYQDNEGAKNAYEADAKFLKNNGYGKPVTAK
                     TIGESSIMLKKKDTDGITYNLLFLKNNVFGAVSAKYKKEMPDNVDHLTGLAEKIEGKI
                     K"
BASE COUNT          521 a          413 c          333 g          525 t
ORIGIN      
        1 aataataact cctttctcgc caggaacagc gtttatatta aaatcaagcg caaattcctt
       61 aagttcatct tccctgagtt ctcttacggt ctcaaaagct gattttgcct cagaaagagc
      121 ataccgcttt atcaggtatc cgtcattgag gcacgagaca aggattcttg caacaggata
      181 tgagagaagt tccttttctg cgctcaccct gtcaggagac acatttcgga tgatgccctc
      241 cccgattgat tccagcaccc ggttctttcc acgttcccga acctgttcaa ataccctttc
      301 ggaaaaaagt tcatcaagtt tgaaatccag cgcttctaca tattttgcgg cagcattgac
      361 aaaaggaaaa taagcaaact tgaaaacatc catgcttatt ctgattcaac tcttggagcc
      421 agcatataac ttacttcgcc gtggccttca gcgatcttta tccttatctt caggggaaaa
      481 tcctttccaa ggtctatcgt gacttcacct gatttcccaa ggatcttact catgtccgag
      541 agataatcaa gggagaataa tgattttgca tcggtcgcct ggaggtctat caactgctct
      601 tttcccaatt ccacaaggac tttatccgta tctccttccg cttccatgaa gaaaatatct
      661 cctctaactc ccatggacat gtagtcgctc actttttcag cggcttttac ggcccttttt
      721 atatcctcgc ctcgtataac gatatgcaca ggaaggtcaa ggttaggaac tttaggttct
      781 ttccttatgg atgaaggatc aagcagggac attgtgtaag caagccctcg catcctgaca
      841 atgagcttat ggctggcttc atcaagctct agctcaatga tatcgctctt atctgccatc
      901 tccattaccc cggtcaattt tgtgagatcg atgccaagtt ccccttctgt tgcctcaaat
      961 gaatcaaaag catcttttga aagctcaaaa ctcaccatgg cgacattggc cgggtcgact
     1021 gcctttactg ataaaccctc aggcgtaatt ctgaatctcg cttcatctac tagtgtggat
     1081 atcgattcta tggaatcttt cagtttttcc gctcctatta atgccttaaa cattttattc
     1141 ttcctctttt aaactgtgtt gggcattaat aggtaattct ctctatttta ttctatctat
     1201 taaaagtcga tactttcata tccatcctct gcatttactg caaaacatga caggacgttc
     1261 tatcgatatc gccctgaccc aggccgaggc aggagaaaaa acggaaacag tctatgaaaa
     1321 tgtatgcacg ctttcaacag aaccagaact atgctttaac tatgcaacaa cggatgacct
     1381 taagaaatgg ggtttttcgg aaggatgttt tagttgtttg ctaaaagacg agaaggatac
     1441 gatcatacag gtgattacaa catatcagga taatgaaggg gcaaaaaatg cctatgaagc
     1501 cgacgctaaa tttttaaaaa ataacggata tgggaaaccc gtaacagcaa aaaccatcgg
     1561 tgaatcgtct atcatgctaa agaaaaaaga tactgatggg atcacctata atctcctgtt
     1621 tttgaaaaat aatgtatttg gcgcggtcag cgccaaatac aagaaagaaa tgcccgataa
     1681 cgttgaccat cttacagggc ttgcggaaaa aatagagggg aagataaaat aattgagtgt
     1741 taaaataata ttcaattcac ccgaacattc ccgctacttc ttctcttgtc tc
//
LOCUS       DATGBN010000277         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_99600, whole genome shotgun sequence.
ACCESSION   DATGBN010000277 DATGBN010000000
VERSION     DATGBN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99600"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VKL21_06745"
     CDS             <1..1086
                     /locus_tag="VKL21_06745"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMB45502.1"
                     /translation="ISLIEELGLANRLEWLKGTTGYYFDGKIYPMNTPIEILAALPLM
                     DVIRLTWLVLKAKTIKDRTPFDNITAKEWIIDTAGESVYNNFFLPLLSSKFGDNKEKV
                     SAAWLLGRVQIRSNRGTKGERLGYMRGGFELLLEKMVENIRNRGGIIKQGNVSHIEMS
                     GNSIKGVVVDGKLIECDRVISTVAPHVLEKIMDTKLLGLNMDISYQGTACALFGLTEK
                     IMDDIYWLNIKEDVPFGAVIEHTNFIPGSDYGERLMYVTTYFQDQESVLWKSNDEEVI
                     ELYMKGLEKLFPGFRKKMKWWRLRRDIDTAPVYEVGYGKKILPFETRIKGLFLAGMFS
                     DSNYPERSMNGSILAGYKCSEAVLKHS"
     gene            complement(1094..2242)
                     /locus_tag="VKL21_06750"
     CDS             complement(1094..2242)
                     /locus_tag="VKL21_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HMB45503.1"
                     /translation="MSNYRKILGLWDLVSFGVGGTIGSGIFVVPGIAAGIAGPGSLLA
                     WMLVAISATCVMLSLGWASSKYPSTGAFYSIYSSVFGKHLSTTIVFFYLISAVFGIAT
                     IAAGIGQYVFYLGYQDVLWIEIGVIVFFGIVNMIGTRPSGNIEMVLTILKTIPLVILA
                     FLLLPHMKHENFTPFFTGNTGDFLRTVIIVYWCFTGFEISAIPADETRNKNDIFKSLV
                     IVMLVVTFVYLFLNISLIGSLGSQLIASSSAPMAKAASIVFQASGNIMAYIGIIAMMS
                     ALNAYLLATSRVLQNISLKYHLPLVPDIGKNGVPYAAIMISTIAGASLLLFSNHFEQL
                     ATISVITTLLPYLFICASALRMFDSFKIRIISTLGLLSTMVILAASLL"
     gene            2321..2905
                     /locus_tag="VKL21_06755"
     CDS             2321..2905
                     /locus_tag="VKL21_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HMB45504.1"
                     /translation="MSFEAKNFESLLGTKGLSDQLLKNHFTLYQGYVTNTNKVADALG
                     AMAKEGKTASPEYAELKRRFGWEWNGMRLHEYYFGNLIKDGKALDKNSNLFRKIVEDF
                     GSYENWEKDFKATGAMRGIGWVILYYDGSRLFNTWINEHDVGHLSGASPLLVMDVFEH
                     AYMLDYGLKKADYIEAFFRAIDWTIDPKMDSWIK"
     gene            2944..>3215
                     /locus_tag="VKL21_06760"
     CDS             2944..>3215
                     /locus_tag="VKL21_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45505.1"
                     /translation="MAKMWTSNEEFDFETARDHASKLVLLGSAHRAGIFEALLEEKDI
                     PSLKLVLNADERALFIVLEALCTLGYVHKRQDRYSITEKARVHFIE"
BASE COUNT         1037 a          579 c          753 g          846 t
ORIGIN      
        1 atttctttga tagaagaact gggtcttgcg aatcggcttg aatggttaaa aggcacaaca
       61 ggatattatt ttgatggtaa aatatacccc atgaatactc caattgagat ccttgcagct
      121 ttaccgctca tggatgttat acggttgact tggcttgtgc taaaagcaaa aaccataaaa
      181 gacagaaccc cttttgacaa tatcactgcg aaagaatgga taatagatac agcaggtgaa
      241 tctgtttata ataatttctt ccttccactg ctgtcaagta aatttgggga caataaagaa
      301 aaagtgtcgg ctgcatggct tctcgggcgg gtgcaaattc gctcgaaccg gggaacaaag
      361 ggagagcgat tgggttacat gcgcggcgga tttgagttgc tccttgagaa aatggtggaa
      421 aatatccgaa acaggggagg aattataaaa cagggcaatg tatcgcatat tgagatgtcc
      481 ggtaattcaa taaagggagt tgttgttgat gggaaactta tcgaatgcga cagggtgatt
      541 tcaaccgttg cccctcatgt tcttgagaaa ataatggata caaagctcct gggacttaat
      601 atggatatca gctaccaggg cactgcgtgc gcgcttttcg gattgaccga aaagatcatg
      661 gatgatatct actggctcaa tatcaaagag gacgttccct ttggcgctgt catagaacat
      721 actaacttta ttcctggatc ggattatgga gaacgtctca tgtatgttac aacatatttc
      781 caggatcagg aaagcgttct gtggaaatca aacgatgaag aggtcatcga gctgtatatg
      841 aagggacttg aaaaactttt cccgggattt cgcaaaaaaa tgaaatggtg gcgccttcgc
      901 agggatattg atacggcgcc agtttatgag gttggatatg ggaaaaaaat cctgcctttt
      961 gaaaccagga taaaagggct tttccttgcc ggtatgttct cggattcaaa ttatcctgaa
     1021 aggagcatga atggctcgat actggcagga tataaatgtt ctgaagctgt tttaaagcat
     1081 tcttaggagt agttcacaaa agggatgctg caagaataac cattgttgaa agaagcccca
     1141 gagttgagat tatccggatc ttgaaactat caaacatcct taaagcagaa gcacagatga
     1201 ataaataagg caaaagagtg gttatgacag aaatagtagc aagctgctca aagtggttgc
     1261 tgaaaagcag aagtgacgct cctgcaatgg tgctgatcat tatcgctgca taaggaacgc
     1321 cattttttcc gatatcagga acaagaggaa gatgatactt aagtgaaata ttttgcagca
     1381 cccttgaagt ggcaaggaga taagcattaa gcgctgacat catggcaatt atgccaatat
     1441 aagccattat gttgcctgaa gcctggaata caatagaagc cgcctttgcc attggggcgg
     1501 aggatgatgc aattaactgg ctgccaaggc tgcctatcag ggaaatatta aggaataaat
     1561 atacaaatgt gacaaccagc attacaatta ccagggattt gaaaatatca ttcttatttc
     1621 ttgtttcatc cgcaggaatt gcgcttatct caaaaccggt aaagcaccag taaacaatga
     1681 tcacagtacg aaggaaatca ccggtatttc cggtaaaaaa aggagtgaaa ttctcatgct
     1741 tcatatgtgg aagcaataaa aaagccagta tgactagcgg aattgttttc agaatggtca
     1801 gtaccatctc gatattcccc gatggtcttg taccgatcat attaactatt ccgaaaaaaa
     1861 caattacacc tatctcgatc catagtacat cctgataacc cagataaaaa acatattgac
     1921 ctatacctgc tgctatagtg gcaattccaa aaacagcaga gatcaaatag aaaaaaacta
     1981 ttgttgttga aagatgtttt ccgaatacgc tcgaataaat ggaatagaaa gcgcctgtag
     2041 aaggatattt tgaggaagcc catccaagcg atagcatgac gcaagttgca gaaattgcca
     2101 caagcatcca tgcaaggagg gaaccaggac cggctattcc tgccgctatt cccggaacta
     2161 cgaatatacc tgaaccaatg gttccaccga ccccgaaact gaccagatcc caaagaccca
     2221 ggatttttct ataatttgac atctatacaa agaaacttac aggataataa agtataagaa
     2281 tgttcgttca tatatgaata acttgaagtg gtgttagaat atgtcatttg aagcaaagaa
     2341 ttttgaaagc cttttaggca cgaaaggttt gagcgatcag ttgttgaaaa accattttac
     2401 tttataccag ggatatgtta caaatacaaa taaagtggca gatgcccttg gtgcgatggc
     2461 aaaagaagga aagacagctt cgccggaata tgctgagtta aagcgcaggt ttggctggga
     2521 atggaatgga atgaggttgc atgaatatta tttcgggaat ttgatcaaag acgggaaagc
     2581 gctcgataag aattcgaatc tattcaggaa aatagttgag gatttcggtt catacgagaa
     2641 ctgggagaag gatttcaagg caacaggcgc catgagaggc ataggatggg taatattata
     2701 ttatgacgga agtcggctct tcaatacatg gatcaatgaa catgatgtgg gccaccttag
     2761 cggggcatcg cctcttctgg tcatggatgt gtttgaacat gcttatatgc ttgactacgg
     2821 attgaaaaaa gcagattata ttgaggcatt tttcagggcg atcgactgga cgatcgatcc
     2881 gaaaatggat tcctggatca aatagaatga gtcgataatc attttttttt actttgttat
     2941 tgcatggcaa aaatgtggac gtctaatgaa gagtttgatt ttgaaactgc aagagaccat
     3001 gcaagcaaac ttgttttgct tggctctgcg cacagagccg ggatatttga agctctcctt
     3061 gaggagaaag atatcccatc actgaagctt gtgctcaatg ccgatgagag agccctgttc
     3121 atcgtgcttg aggcgttgtg tacccttgga tatgttcata agagacagga caggtattca
     3181 attactgaga aagcccgggt ccacttcatc gaaga
//
LOCUS       DATGBN010000278         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_21249, whole genome shotgun sequence.
ACCESSION   DATGBN010000278 DATGBN010000000
VERSION     DATGBN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21249"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VKL21_06765"
     CDS             <1..255
                     /locus_tag="VKL21_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45506.1"
                     /translation="GYLLEAVPVRYRNEAITVALNDRDVATSVRNSGDPSVKRILPGT
                     SQKFYVSKTLLSVTWDGISALVDPDERKVVQVWKESETKK"
     gene            283..819
                     /locus_tag="VKL21_06770"
     CDS             283..819
                     /locus_tag="VKL21_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HMB45507.1"
                     /translation="MSFVERVKGIQSNKIIYIFEGLDNMPSSTQSLMTVITIVGALIV
                     PFITWIIVTGVIHLLSMAAGGEGKFYPQMMTIVGFSMLPLIFNGIISIGLFSMIEPQT
                     INVADPTAMKELYSNSYFLVSMLVSIIIEVWSTIILYFGIMSAHKLSSNSSAIIAAIP
                     LVFSVISLIWSFRGIGIL"
     gene            828..1490
                     /locus_tag="VKL21_06775"
     CDS             828..1490
                     /locus_tag="VKL21_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB45508.1"
                     /translation="MDIENVSKSYKLGKTNVPVLNDINLTINEGEFAAIMGPSGSGKS
                     TLMNLIGCLDRPTSGKILITGMDVSKLSDIELARIRGKRIGFVFQTFNLISRLSAQKN
                     VEMPMVYQDVPGKTREKRAKVLLEMLGLSERARHKPPELSGGQRQRVAIARALANEPE
                     IILADEPTGNLDSKTGQEIMQIFDKLHVDGRTILMVTHDAELARNCDRIIRLKDGMIE
                     NG"
     gene            1483..>1850
                     /locus_tag="VKL21_06780"
     CDS             1483..>1850
                     /locus_tag="VKL21_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB45509.1"
                     /translation="MDDIIEKTIKVSRLSKSFNGQNVVRDISFEIKKGEVFGLIGPNG
                     AGKTTIIRMLLDIMRPDSGEIRILNDGLSEEIKNRIGYLPEERELYKKLTVYSTTDSR
                     NYLFFNGLFGICKRNGRRWD"
BASE COUNT          571 a          345 c          436 g          498 t
ORIGIN      
        1 ggttatcttc ttgaagcggt acctgtgagg tacagaaatg aagctatcac ggttgcactt
       61 aatgatcgcg atgtagcaac atcggttcga aattccggcg acccttccgt gaaaaggata
      121 ttacccggga catcccaaaa gttttatgtg tctaaaacgc ttttaagtgt gacgtgggat
      181 gggatttcag ccctcgttga ccctgatgaa agaaaggtag ttcaagtatg gaaagaaagc
      241 gaaactaaaa aatgatgcta atacctgaaa atttggagac taatgagctt tgttgaaaga
      301 gtaaaaggga ttcaatcaaa taagatcata tatattttcg aagggcttga taatatgcct
      361 tcttcaacgc aatccctgat gaccgtaata actatagttg gcgctttgat agtaccgttt
      421 attacctgga ttattgttac aggagtaatt caccttttgt ctatggctgc aggaggtgaa
      481 ggcaagttct acccacagat gatgacgatt gtaggattca gtatgcttcc cttgattttt
      541 aatggaatta tcagcatagg gcttttttcc atgatcgagc cccagacgat aaatgtcgct
      601 gatccaacgg caatgaaaga attatattca aattcatatt tcttggtttc aatgcttgtt
      661 agtataataa ttgaagtatg gtccacaatt atattatatt tcggaataat gagcgcgcat
      721 aagctttcat ccaattcatc tgcaataatc gcagcaattc ccctggtctt cagtgtcatt
      781 tcattaatat ggagtttcag gggtatcggc atcctatgag gacggtattg gatattgaaa
      841 atgtttccaa atcatacaaa ttggggaaaa ccaatgttcc agtattgaac gatatcaatc
      901 tcaccatcaa tgaaggtgag tttgcagcaa taatggggcc atccggtagc ggaaagagca
      961 cgcttatgaa tctaatcggc tgccttgata ggcccacatc ggggaaaata cttattacgg
     1021 gaatggacgt ttcaaagtta tccgacattg aacttgccag gatacgcggg aaaaggatag
     1081 gttttgtttt ccagactttc aatctgatat cgcgcctcag cgcccaaaaa aacgtggaga
     1141 tgcctatggt ttaccaggat gttccaggga agaccaggga aaaaagagct aaagtgcttc
     1201 ttgaaatgct gggtcttagt gaaagggcac gccacaaacc ccctgaactc tcaggcggcc
     1261 agcgccagag agtcgcaata gcacgggcgc ttgcaaatga gcctgagatt atcctcgctg
     1321 atgagcctac aggaaatctt gattcgaaaa caggacagga aataatgcaa atatttgaca
     1381 aactccatgt tgatggaaga acgattctca tggtcaccca tgatgccgag cttgcccgga
     1441 attgcgacag gattatcagg ttaaaggatg ggatgatcga gaatggatga tataatagag
     1501 aagacgataa aagtctcccg actatcaaaa tccttcaatg gacaaaacgt ggtcagagat
     1561 atctcatttg agatcaaaaa aggcgaggtc ttcggactta tcggtcccaa cggggcggga
     1621 aagaccacga taatacggat gcttcttgat ataatgagac cggattcagg agaaataagg
     1681 atcctgaatg acggtcttag tgaagaaata aagaaccgga tcggttattt gccggaagaa
     1741 cgggagctct ataaaaaact cacggtttat tccactactg atagccggaa ttatttattt
     1801 tttaatgggc tatttggtat ttgcaagcgt aatggcaggc gttgggacag
//
LOCUS       DATGBN010000279         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_21257, whole genome shotgun sequence.
ACCESSION   DATGBN010000279 DATGBN010000000
VERSION     DATGBN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21257"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..661
                     /locus_tag="VKL21_06785"
     CDS             362..661
                     /locus_tag="VKL21_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45510.1"
                     /translation="MNKNGESIFFNASVIGLIVGNFAGGKDKEKGGSGLISPFGIDLA
                     IMLGWTFGIFKTFGFTLTPAIAIGRMGFIVISFGIHFKQRVGFGIVIPIFPIWGY"
     gene            826..1416
                     /locus_tag="VKL21_06790"
     CDS             826..1416
                     /locus_tag="VKL21_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HMB45511.1"
                     /translation="MAYEVKNYDKLLGTKGFSDQLLKNHFTLYQGYVTNTNKVADALS
                     AMAREGKTATPEYAELKRRFGWEFNGMRLHEFYFGNIIKGGKEIDKNTNLYKKIVADF
                     GSYDNWEKDFKATGAMRGIGWVILSYDGMEGRLFNTWINEHDGGHLCGAKPLIVMDVF
                     EHAYMIDYQLKKADYIEAFFKAIDWTIDPEMDSWIE"
BASE COUNT          522 a          246 c          329 g          458 t
ORIGIN      
        1 tgaaattcat aattttttgt ttatgcaagg tttaccatta cttttgaaga ggatactaaa
       61 aatctgtcta aaaagttctt tgagtggatg gagcaagaat atatatataa gttattgcaa
      121 ttaaattaat aagatcggga tagtgattat atgggaaact tcaacaacaa aaaaagtact
      181 gaaccgaaca agtgtgaaca acccccacca aatcctaagc ctatccctgt acctgaacca
      241 atccctgaac cgactcctga acctaatctg gcccactgcc aaatattctt tgactatgag
      301 ggatcaatag tcgtttataa taaacagact taataaccta taatcttaag attaatctaa
      361 tatgaataaa aatggtgaat ctatattctt taatgcttct gttatcggtt tgatagtagg
      421 caattttgca ggtggaaaag ataaagaaaa gggcggtagt ggacttattt ccccattcgg
      481 tatcgacttg gcaataatgc ttggatggac atttggaatc ttcaaaacat tcggatttac
      541 tttgactcct gcaatagcaa tcggtagaat gggttttatc gtaatctctt tcggaattca
      601 ctttaaacaa cgtgtcggat ttggaattgt aattccaatc ttccctattt ggggttattg
      661 aaaaagtaaa tctgactatc ggttctttct gtaaatttca ccatggactt agaagattgg
      721 tgatttgatg ataaagtccg ctaaaaattc tacaaaacat gaaattcaca ttgataaaaa
      781 gttatttaaa tcattgcagt tatcttaata attggggttt tttacatggc atatgaagtt
      841 aaaaattatg acaaactcct gggaacaaaa ggtttcagcg accagttatt gaaaaatcat
      901 ttcactttgt accagggata tgtgacgaat acgaataaag tagcagacgc attgagcgcc
      961 atggccagag aaggaaagac agctactcct gaatatgctg aattaaagcg gaggttcgga
     1021 tgggagttca atgggatgag gctacacgaa ttttattttg ggaacatcat taaaggcggg
     1081 aaagaaatcg ataagaatac gaatttatat aaaaaaattg tagcagattt cggttcatat
     1141 gataactggg aaaaggattt taaagcaaca ggcgccatga gaggtattgg ttgggtgatc
     1201 ttatcctatg atgggatgga gggccgtctg ttcaatacgt ggatcaatga acatgatggt
     1261 ggacaccttt gcggggcaaa acccctcata gtcatggatg tctttgaaca tgcttacatg
     1321 atcgattacc agttgaagaa agcggattat atcgaggctt ttttcaaagc cattgactgg
     1381 actatagatc ctgaaatgga ttcatggatc gaataaattg aaaaactgtc cttgataagt
     1441 ggaaaagaat atgagtatag accctgattt catgaaaaag ccagttgttg gtgaacacaa
     1501 tgggcacaag attagagggg aagtcaaccc tcccgggaaa cttggtgtgc atggc
//
LOCUS       DATGBN010000280         1842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_138498, whole genome shotgun sequence.
ACCESSION   DATGBN010000280 DATGBN010000000
VERSION     DATGBN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138498"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1122
                     /locus_tag="VKL21_06795"
     CDS             124..1122
                     /locus_tag="VKL21_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB45512.1"
                     /translation="MSKYPAGKGSSVAGLTVAVVKDELSGSGYTAQAGAFVLADRGLM
                     VLDEVDKLEKEDFQALNTALEESFIEVHKGGINQKFNTRCPVIALCNPKNSRFEPEEP
                     FTKQISIPGDTLSRFDLVFKILDIPDKDKDREIAQHQARQWARYGGSDQEQPEERSLS
                     QEKLSKYLQYAKMIKPRTTPEADQILLDYYLTLRKPDDSGTIATTARQFNGLYRLTKA
                     IAKLRLSDTCSKEDAEKTIQIHKASLEALRDPTTGKIDNDILSGMGKSQRDRYRQIRD
                     IIQDLQGNNGSSAHYNDIIAKAEEKHIGRDQAKSDFDYLKHTGEIIEVSNGFYRVI"
     gene            1280..1405
                     /locus_tag="VKL21_06800"
     CDS             1280..1405
                     /locus_tag="VKL21_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45513.1"
                     /translation="MKSNVITIHVQGFKQEYPSWKHHKDIEKVGRKTLLRALEEK"
     gene            1625..>1842
                     /locus_tag="VKL21_06805"
     CDS             1625..>1842
                     /locus_tag="VKL21_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45514.1"
                     /translation="MKRILQEHKYRINMAEFSIRKTYLSQLITELNNQSLSGDLTPSE
                     RNKIYNTITNTTKVLNEICKDCTLESLE"
BASE COUNT          692 a          351 c          363 g          436 t
ORIGIN      
        1 ctattttttc aatattcagg cttttaatct catcagggat tgatgtcaca ataaatatat
       61 aattatgtaa tagcacttaa tttttttggt cggtttaatt atattttaga ttcccacttg
      121 atattgagca aataccccgc aggcaagggc tccagcgtgg caggccttac cgtggctgtg
      181 gtgaaagacg agctctcagg cagtggatac acagcacagg ccggggcgtt cgttcttgcg
      241 gatcgtgggc tcatggttct tgatgaagtc gataaactag aaaaagaaga tttccaggca
      301 cttaacacgg ctttggagga gagttttata gaagtacata agggcggcat taatcaaaaa
      361 tttaacacaa gatgtccagt gatagcctta tgcaatccta agaacagcag atttgaacca
      421 gaggaacctt tcacgaaaca aatatcaatc ccaggtgata cactcagtag atttgattta
      481 gtttttaaaa ttttggatat accagacaaa gacaaggaca gagaaatagc acaacaccag
      541 gcgcgccagt gggcacgata tgggggaagt gaccaagaac aaccggagga gaggagttta
      601 tcacaggaaa aattaagtaa atatctccaa tatgcaaaaa tgataaaacc gcgaaccact
      661 ccagaagcag atcaaatact acttgattat taccttacac tcaggaagcc agatgacagc
      721 ggaacgatag caaccacagc gcggcagttt aacggattgt ataggctcac aaaagcgata
      781 gctaaacttc ggttatctga tacatgcagc aaagaggatg cagaaaaaac tatccagata
      841 cacaaagcaa gccttgaggc gctgagggac ccaacaaccg gaaagattga caatgatatt
      901 ctttcaggaa tgggcaaaag ccagcgcgac agatataggc agataaggga tattatccag
      961 gacctccagg gcaataacgg aagtagcgca cattataacg atatcatagc aaaagcggaa
     1021 gagaaacata tagggcgaga tcaggccaaa agcgactttg attatttgaa acacacggga
     1081 gaaattatcg aagttagcaa cggattttac agggtgatat agtgcttaga aaaggcaaac
     1141 atctgtatac atatgtatac atacctttga aaaacctaag tagctgtatc tctttttttc
     1201 aaacatacag aactttgacg tcaaacatac agaactttga cgtcaaacat acagaacttt
     1261 caaggaggga attgacctca tgaaatccaa tgttatcaca attcacgttc aaggattcaa
     1321 acaggaatat cctagctgga aacatcataa agatattgaa aaagtaggta gaaaaactct
     1381 ccttcgggca ttagaggaaa aatgaagcaa gaacaaccaa aaaaagacga tagtaattcg
     1441 aacgaacaaa aacaaggact aacccgatac agtgtaaaaa agattcgata tttaaggaca
     1501 aaacatgtat aaaacgccct ttttacccct gcaaagcttt gacccatcgc acaatacggt
     1561 caatttccaa cattacttta tattcttaca agtcaattta acaacacaga ggtactgaat
     1621 aagcatgaaa cgaatacttc aagagcacaa atatagaatc aatatggctg aattttcaat
     1681 aagaaaaact tacctaagcc aattaatcac cgaattaaat aatcaatcct taagcggaga
     1741 tttaacccct tctgagcgaa acaagattta caatacgatt acaaatacaa caaaagtttt
     1801 aaacgaaatc tgcaaagatt gtactcttga atctctggaa aa
//
LOCUS       DATGBN010000281         1970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_487528, whole genome shotgun sequence.
ACCESSION   DATGBN010000281 DATGBN010000000
VERSION     DATGBN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487528"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1013)
                     /gene="cas1"
                     /locus_tag="VKL21_06810"
     CDS             complement(108..1013)
                     /gene="cas1"
                     /locus_tag="VKL21_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HMB45515.1"
                     /translation="MQLVINTRGAYLKKEKNCFLIKNDDKSFEVSADKVDSILITTSA
                     TISTDAIEFAVENNIDIVFLDFHGNPFGRVWHSKLGSTTLIRRRQLEAEASVLGFNLA
                     RGWIVRKIDNQLDFLKDLKKNRVDDKELIEPKIAKITELRETLVGMKGSLEEKRGSIM
                     GNEGMASLNYFDALSSIMPDAWRFNGRSRDPAQDAFNCLLNYGYGVLYSQVEKGCIIA
                     GLDPYMGFLHTDNYNKKSFVFDLIEIFRIHVDRTVMNLFSKKQVHEGLFDKIPGGLYM
                     NKDGKAVLITAINETGNVPDSLAFI"
     gene            complement(1013..1396)
                     /locus_tag="VKL21_06815"
     CDS             complement(1013..1396)
                     /locus_tag="VKL21_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HMB45516.1"
                     /translation="MKQDERYLTKVERFIKEEEFILTHTIENEVTERALLYSLQVSVE
                     ISMDIVAMKVRDMGLKVDDDATNIDKITERGIISHGEANFLKEMNGVRNFVVHRYNQL
                     DMNIVNEALSRMSDLKGIILKVADY"
     gene            complement(1383..1838)
                     /locus_tag="VKL21_06820"
     CDS             complement(1383..1838)
                     /locus_tag="VKL21_06820"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB45517.1"
                     /translation="MITHDKMKQNYQKSTNMHPEELIIRLKKNFEFIKDEAEGIILYG
                     SRAMNTANERSDIDICLIKPQTTGILKRIFQQVGGRYDIKIFEDMPLYVKMKIIENYR
                     IIYGDEPSISYYLYHFRKNWEDMGYRISSNRFKTVTDMTLTRRKWLETR"
BASE COUNT          557 a          421 c          322 g          670 t
ORIGIN      
        1 tatacagctg gactgctcgc tctagcatga gctattgtag tttttaatct ttttcataat
       61 gtagtatatg aaagtttaat tattaaaatt atatgattaa ttatgtatta gataaatgcc
      121 aatgaatctg ggacattacc cgtttcattg atcgctgtta ttaaaaccgc tttaccgtcc
      181 ttattcatgt acaagccacc gggaatttta tcaaaaagcc cttcatgcac ctgtttttta
      241 gaaaaaagat tcataacagt tctatcgaca tgtatcctaa agatctcaat aaggtcaaaa
      301 acaaagcttt tcttattgta attatccgta tgcaaaaaac ccatataagg atcaagtcca
      361 gctattatgc atcccttctc gacctgcgaa tacaggactc cgtagccgta attaagaaga
      421 caattaaaag catcctgtgc cggatcgcgg cttctgccat tgaatctcca tgcatcaggc
      481 atgatactac tgagagcatc aaaataatta agcgatgcca tcccttcatt tcccattata
      541 gaaccccgct tttcttcaag tgatcccttc atgccaacaa gagtttctct aagttccgtg
      601 atttttgcaa tttttggctc aatcagctcc ttatcatcca ctctattctt tttcaggtct
      661 ttcaggaaat caagctggtt atcgatcttg cgcactatcc agccgcgagc caggttaaat
      721 cccagaacag acgcttcagc ttcaagctgg cggcgccgga taagggttgt gctgccaagt
      781 ttagagtgcc agacccgacc gaaagggtta ccatggaaat ccaagaaaac aatatctata
      841 ttattctcaa cagcgaactc gatggcatcc gtgcttatgg tggctgatgt tgttatgagg
      901 atactgtcaa ctttgtctgc tgagacttca aagctcttat catcattctt aatgaggaag
      961 cagttctttt cctttttgag atatgcacca cgcgtattga ttacaagttg catcaataat
     1021 cagccacttt tagaatgata cctttaaggt cgctcattct tgaaagcgct tcatttacga
     1081 tattcatatc aagctggtta tacctgtgaa caacgaagtt cctcacacca ttcatctcct
     1141 ttaagaaatt ggcttctcca tgtgatatta ttcccctttc agtaatttta tcaatatttg
     1201 tggcgtcatc atctactttc aaacccatgt cccgtacttt catggcgact atatccattg
     1261 aaatttctac agaaacctga agcgaatata acaaagcccg ctcagttacc tcattttcga
     1321 tggtatgagt caaaataaac tcctcttctt tgataaacct ctcaactttt gtaagatacc
     1381 tttcatcttg tttcaagcca tttcctcctt gttagagtca tatccgttac tgttttgaaa
     1441 cggttgctgg atatcctata tcccatatct tcccaatttt tcctgaaatg atataagtaa
     1501 taagaaatag agggctcatc gccatatatt atcctgtaat tttcgattat tttcattttt
     1561 acatacagag gcatatcttc gaagatcttt atatcatacc tgcctccgac ttgttgaaat
     1621 atccgtttta agattcctgt agtctgaggt ttgatcaggc agatatcaat atctgaacgt
     1681 tcatttgcag tattcatggc acgggaacca taaagtatga tgccttcagc ttcgtctttt
     1741 atgaattcaa aatttttctt aagccttatt atgagttctt caggatgcat attagttgat
     1801 ttctggtaat tttgtttcat tttatcatga gttatcatat ttgtcaattt tggtatatat
     1861 tattaaagtt aattcccttt catgacaacg gcgccaaatc ctcttgatac ggattttccc
     1921 acaccgagat agtcaggtat gatgaagttc acctggaatt ttccgataaa
//
LOCUS       DATGBN010000282         6968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_293455, whole genome shotgun sequence.
ACCESSION   DATGBN010000282 DATGBN010000000
VERSION     DATGBN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293455"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1563
                     /gene="ltrA"
                     /locus_tag="VKL21_06825"
     CDS             313..1563
                     /gene="ltrA"
                     /locus_tag="VKL21_06825"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HMB45518.1"
                     /translation="MKKWNQQYHDKFKAFALIHKVWDQKNLEKAWKSVKANRGSAGID
                     NITVEQFEQNLQQNLAEIQRLLKEKRYEPNPVLRVMIPKDNGKMRKLGIPTVRDRVVQ
                     QALKNVLESIFEETFLPQSHDYRPNTNAHAAIRKGETFLERGYHWVVDADIEGFFDHV
                     DHQILMDLVCEKVADGRVLSLIESFLKSGIMNEGLFEESTEGTPQGGNLSPLLSNIYL
                     NHFDRRMGDYGYLLLRYADDIVIFCKFESDAQDALKHAKEILEGELKLKLSPEKTKII
                     HARKKGVEFLGFHFNGRWRRPKDKAKKKFKEEIRRRTRRNLPVNLETVIHFLNPVIRG
                     WGNYFKGGTVKKLFEELDGYIRARLRSFKAKRRTWGTILYTLPQSELKNMGLISLSSL
                     LNKSVSCKGKSQTKAVYGKTVRTV"
     gene            1969..3195
                     /locus_tag="VKL21_06830"
     CDS             1969..3195
                     /locus_tag="VKL21_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein domain-containing protein"
                     /protein_id="HMB45519.1"
                     /translation="MSKKILTVALVLLMIIMISLPANAAVTTTKVEIRGSVANASTLA
                     ANGSVAWNYANFAGFWYDLKTNQSTESLAVQAFNGARSIDKEKLWYNTTIAATKFKVA
                     SEKSRDVEYGLDANGANPTSTGAQYYDVVGWMAEKYIGINGKPNKLAKLILEQDTSDK
                     KTLTVGETWEMGGGYSLTAQSIDARSNPRLAWFVLSKDGAKLDDKVLEAASTTAGNGT
                     QGVYTYFANLGGESNVPIFVTYIDSVFSGASSDMVQLKYTWLIDNTITEVKSGDKFGI
                     FKVDSESPLKLKSDSAVSLSKDSTVTLAGNMNFRVSDSADTDVRFYPKLDYVITGGND
                     TKPGNTTTVKGTARPTTNVTSNITAVVPTIPPTVVPTGSETKPAASPTSVPGFEVTFA
                     IAGLLAVAYIVLRQRK"
     gene            3315..3662
                     /locus_tag="VKL21_06835"
     CDS             3315..3662
                     /locus_tag="VKL21_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45520.1"
                     /translation="MKLSKQSLIIATTVPLVFGVLILYNFISAINILRPVAPSTALIY
                     SQLYVIGLLLILTFAVYWCIMMYLIYLLQENLLVLDKNLDRAADVGAKHLSESKNLLN
                     QVINILKRYETVQ"
     gene            3760..4560
                     /locus_tag="VKL21_06840"
     CDS             3760..4560
                     /locus_tag="VKL21_06840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB45521.1"
                     /translation="MDKIACSVNSFYERYPYPSLPIRNEHDLAGKLHANVMSKILFTA
                     GLESASLSGKEILDAGCGTGEKSCYFAYHGAKVTSLDLSCSSISKGKKLAQMFDLKVE
                     FMRCDIGDFKTEKKFDHIFSLGVLHHTNDPYGRFRALADLCKPGGTITIGLYNRYGRF
                     LHRAKRIWIKLNAGEDIDRRMSFVESSIYGRKFRNMHEQAYAADKYANPYESYHSVIE
                     VIDWFNKNDISYIGSYPHTDIGIIRSFLTQLKWFKSRNGFFIISGRKN"
     gene            4557..5357
                     /locus_tag="VKL21_06845"
     CDS             4557..5357
                     /locus_tag="VKL21_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HMB45522.1"
                     /translation="MKEKWRFIDLDKIDGFKSAALFETIAKYVGGNVSPETILFWRAR
                     SPVVYLGYHQCVEDEINVDHCRNHDIGIVRRILGGGCGFCDENQILFSCIGKEGGIIS
                     PDIQAAYIKVLGAVVNALRTLGTEGEIEPARNAVYSKGRKISGNAQGRLDGAVLINGS
                     LLMDFDFELMDRVLKNPTKNLYPVANAREGMITLKDIGITDIDRAKKALRDGFERALG
                     ITTYEGMLAQNEINTANQLLEKYQRHDWTYRMDIKRAQRKRFSVLSPQ"
     gene            complement(5311..6513)
                     /gene="larC"
                     /locus_tag="VKL21_06850"
     CDS             complement(5311..6513)
                     /gene="larC"
                     /locus_tag="VKL21_06850"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HMB45523.1"
                     /translation="MNTILFDPFCGASGDMTIGALIDLGADAGKTREAMELAANVDVK
                     ISRSNRKGIRACSVEVSIKKEGCMAFPEIIARIRSLDIPPAIIMDAISVFNILGKAEA
                     RIHGTTLDKLHFHELGQEDAIADIIGACTAFHDLGLNDTRIYCAPISVGKGFTEFSHG
                     KFPVPAPAALEILKEYFLPWQMGPVDGELLTPTGSALLAHFVNEKGFCPAMKTQKIGY
                     GAGSKDLFVPNVLRIIRGEIDDALISDMIEMLETNVDDVTGQVLGNLIEELLKSGARD
                     VSIIPATMKKGRSGQIIQVIAKPEDSAGLARKIIEETGSLGVRIIPIKHRLIAHREMG
                     KVPIILNGKEFQITVKVARDLRGVLLNISAEFEDCKRASGESGIPVRDVIRLTEEKAR
                     RTFSAAPV"
     gene            complement(6532..>6968)
                     /locus_tag="VKL21_06855"
     CDS             complement(6532..>6968)
                     /locus_tag="VKL21_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45524.1"
                     /translation="APTITATPTVTVAPTITATPTVTVAPTITAAPTVTTAPTVTATS
                     TVAATPTVTVAPTVTATSTVAATPTVTATSTVAATPTVTAAPTVAATPAPTTSSPATP
                     TITPEIIPTTEETPTITILPFSNGSVLNGTNFTLELWENKSL"
BASE COUNT         2125 a         1388 c         1585 g         1870 t
ORIGIN      
        1 cctttcggga aagtcagagc gatgcgttat cagacgtcgg acgaactcat agtagcaatg
       61 aaagtcaaac caatgacaaa ccgaacctgt gccgcagggt gcagggggcg acggttcgag
      121 gataaaactt gactcagcga aggggttcta gtctggatag agtagaggtg aacagcacga
      181 taacaccaat cggattaaaa gaaagatacc agaaaggtat gcaccaccaa aacggtagtg
      241 aaacgctctc tggtgacagt atgtgaagat agtggaacaa ctgctggaag ccgaaaccga
      301 tggagacagt aaatgaagaa atggaatcag caataccatg acaaatttaa ggcatttgcc
      361 cttatacata aagtatggga tcaaaagaac cttgaaaagg catggaaaag cgtaaaagca
      421 aatagaggaa gtgccggaat cgataacata accgttgaac aattcgaaca aaatctacaa
      481 cagaatttag ccgaaataca gagactgctc aaagagaaac gttatgaacc aaacccggtc
      541 ttaagagtga tgatcccaaa agacaacggg aagatgagga aacttgggat tcctacagtg
      601 agagacagag tagttcagca ggcattgaag aacgtacttg aatccatctt tgaggaaaca
      661 ttcctgcccc agagtcatga ttacagaccc aacactaatg cccatgcggc aataaggaag
      721 ggtgaaacat tccttgaaag aggctaccac tgggtagtgg atgccgatat agagggattc
      781 ttcgaccatg ttgaccatca aatattgatg gatcttgtat gcgaaaaagt cgcagacggt
      841 agagttcttt ctctcataga atctttcctt aaaagcggaa taatgaatga gggtttattt
      901 gaagagagta ccgaaggtac tccccagggc ggaaatctca gtccactgct gtcgaatatt
      961 tatctaaatc atttcgacag aagaatgggt gattatggat atctgctgct tcgttacgcc
     1021 gatgacatcg ttattttctg caaatttgaa tcagacgcac aagatgcgct taaacatgca
     1081 aaggagattc tggagggaga gttgaaattg aaactcagtc ctgagaagac aaaaataata
     1141 catgcaagaa agaaaggggt tgagtttctg ggtttccatt ttaatggacg ctggagaaga
     1201 ccgaaggaca aagcaaagaa aaagttcaag gaagaaataa ggcgacggac aagacgcaat
     1261 ttgcctgtaa atcttgaaac ggtaatacat ttcctcaatc ctgtaattcg gggttggggg
     1321 aactacttta aaggcggtac tgtgaagaaa ttgttcgaag aacttgatgg ttatataagg
     1381 gcacgtttga ggagctttaa ggccaaaagg cgtacctggg gaactattct atacacgctt
     1441 cctcaatcag agcttaaaaa tatggggctg atttcgcttt cttcgttact gaataaatcc
     1501 gtctcctgca aagggaaaag ccagacgaaa gccgtgtacg gaaaaaccgt ccgcacggtt
     1561 tgatgaggag agggaggttg ttaagatctc tcttttactc tacaatattt catgctccat
     1621 gatcggtaaa ttgtcagata caatcataaa tctggcgttt ttaattacta tcaaatcgac
     1681 aataagtgaa gtccgaatcg tcaattgcgc ccatgcatgg ctgttttgac cacaaaagat
     1741 ttaaaagggt acttactata aacggattgt aatccataac tggatttaat agagggtgat
     1801 gaactagcct ttgatggcgg ccattaaatc ggttgttcag gcaaagacgg tttagcgatg
     1861 caggcggaac gaaagttcgg tctttgataa ctatcactcc tggccgtaaa atcggcatat
     1921 ctcgaaggag aaaaaaatat aaccataata atatggaggt aaagaaacat gagtaagaaa
     1981 atattaacag ttgcattagt tttgttaatg ataattatga tatcattacc agcgaatgca
     2041 gcggttacaa ctacaaaagt cgaaataaga ggctcagttg ctaatgcttc aacccttgca
     2101 gctaatggaa gtgttgcctg gaattatgca aattttgcag gtttctggta tgatctaaaa
     2161 actaaccaat caactgaatc gttggcagta caagcattta atggtgccag aagcatagat
     2221 aaggagaaac tctggtacaa tacaaccata gcagccacaa agttcaaggt cgcatccgaa
     2281 aagagcagag atgtggaata tggacttgat gcaaatggtg ccaatccaac cagcacaggt
     2341 gcacagtatt atgacgtagt tggctggatg gcagaaaagt acattggtat caatggaaag
     2401 cccaacaagc ttgcaaaact tatattagag caggatacct cagacaaaaa gacattgaca
     2461 gttggtgaga catgggaaat gggcggcggt tattcactta cggcccagtc aattgatgcc
     2521 cggtctaacc cgagacttgc ctggtttgtt ctgagcaagg atggagccaa gctcgatgat
     2581 aaggtccttg aagctgccag tacaaccgcc ggaaatggca cacagggtgt ctatacatac
     2641 ttcgcgaacc ttggtggcga atctaatgta cccatcttcg tgacatatat tgacagcgtg
     2701 ttctcaggag cttcctctga tatggtgcaa ctcaagtaca catggctgat cgataacact
     2761 atcactgagg tcaagagcgg agataagttt ggcatattca aggttgattc cgaatcgcca
     2821 cttaaactca agagcgactc tgcagtgtcc ctgtccaagg atagcacagt tactcttgca
     2881 ggcaatatga atttcagggt ttcagacagc gcagacacag atgtcagatt ctaccccaag
     2941 cttgactacg tgatcacagg gggaaatgac accaaacctg gaaacactac tacagtaaag
     3001 ggaactgcaa ggccaacaac aaacgtgacc tcaaacatca cagctgttgt gccaacaatt
     3061 ccaccaaccg ttgtaccaac cggatcagag acaaaaccag cagcatcacc aacatcagtg
     3121 cctggctttg aggtaacgtt tgcaattgca ggcttgcttg cagtagcata catcgtgttg
     3181 agacaaagaa agtaaataaa taaattcgaa gggagcaatc ccttcacttt ttttttaatt
     3241 aaattataaa agatatactt aaatagataa atgtcataaa aagacatggg aagaaaactc
     3301 ccggaggaaa ctgaatgaag ctgagtaaac aaagtttaat tattgcaaca actgtaccgc
     3361 tggtatttgg agtattgatc ctttataatt tcattagtgc aataaacata ttgcggccgg
     3421 tagcgccatc taccgcattg atctacagtc aattatatgt tatcggactc ttattgatcc
     3481 tgacttttgc agtatactgg tgcataatga tgtatctgat atatctgctt caggagaatc
     3541 tgctcgtgct ggacaagaat cttgacaggg cagcagatgt gggcgcaaag cacctttcag
     3601 agtcaaaaaa tcttctgaat caggttataa acattctaaa aagatacgaa acagtccagt
     3661 gatactcgta taaatacagg tgactaaaga agggagtgat cccttcacag tacttttttt
     3721 gagcaaacct ataaatcctc taattcaaac aatgggttaa tggataaaat cgcatgctca
     3781 gtaaattcat tctatgaacg atacccatat ccctctcttc caattcggaa tgaacacgat
     3841 cttgcaggta aacttcacgc gaatgtgatg tcgaaaattc tttttactgc gggattagag
     3901 tccgcttctc tttcaggaaa agaaattctt gatgcgggat gtggcacagg agaaaaatcc
     3961 tgctattttg catatcatgg agcaaaggtg acatctttag acctgagctg ctcctcaatt
     4021 tcgaagggaa aaaaacttgc gcaaatgttt gatctgaagg tggaatttat gagatgcgat
     4081 atcggtgatt tcaaaaccga aaaaaagttt gatcacatct tctccctggg ggtgttgcat
     4141 catacaaatg acccttatgg ccgattccgg gcgcttgccg acctgtgcaa acccggcgga
     4201 acaataacta taggattata taaccgatac gggcgttttc ttcacagggc aaaaaggata
     4261 tggataaaac tgaatgcagg ggaagatatt gaccgtagaa tgtcctttgt tgagagttca
     4321 atctatggac gaaaattcag gaacatgcat gaacaggcat atgcggctga caaatacgcc
     4381 aatccttacg aaagttacca ttcggtcatt gaagtgatcg actggttcaa taaaaacgac
     4441 atctcttata taggatcata tccccatacc gatattggca taattcgatc ttttttgact
     4501 caattgaaat ggttcaaaag cagaaacggg tttttcataa tttcaggaag gaaaaattga
     4561 aagaaaaatg gcgttttatt gatcttgata aaatagatgg cttcaaaagc gcagcgctgt
     4621 ttgaaactat tgcaaaatat gtgggtggaa acgtttctcc tgaaacgatc cttttctggc
     4681 gcgccaggtc gcctgtagta tatcttggtt atcatcaatg tgtagaggat gaaataaatg
     4741 ttgatcattg cagaaaccat gatatcggga tagtgcgcag gatcctgggg ggaggatgcg
     4801 gattttgtga tgagaaccag atacttttct cctgtattgg caaagagggc ggcattatat
     4861 ctcccgatat tcaggctgct tatataaaag tacttggcgc agtggtgaac gctcttcgta
     4921 cccttggaac tgaaggtgaa attgaacctg cacgaaatgc tgtatattca aaaggaagaa
     4981 agatctcagg aaatgcacag ggacgtcttg atggtgcagt tcttatcaat ggaagcctcc
     5041 tcatggattt tgattttgag cttatggaca gggtacttaa aaaccccaca aaaaatctat
     5101 atcctgtggc taatgctcgg gaagggatga taaccctgaa agatattgga ataacagata
     5161 tcgatcgtgc gaaaaaagcc ctccgggatg gttttgaaag ggcgcttggc ataaccacat
     5221 atgagggaat gctggcacaa aacgaaatca atactgcaaa tcaattattg gagaagtacc
     5281 agcgccatga ttggacatac aggatggata ttaaacgggc gcagcggaaa aggttctccg
     5341 tgctttctcc tcagtgagcc ggataacatc ccttacagga ataccggact ccccggatgc
     5401 ccttttgcaa tcttcgaact cagctgaaat atttagcaaa acacctctta gatccctggc
     5461 aactttcact gttatttgaa actctttgcc attcaagata attgggacct tacccatttc
     5521 tctgtgtgct atcaacctgt gttttatcgg tattatcctt acgccaagag aacctgtttc
     5581 ttcaatgatc ttccgtgcaa gccctgctga atcctcaggt tttgctatca cctggataat
     5641 ttgaccgctt cgtccttttt tcatagttgc gggtatgatc gagacatctc ttgcgcctga
     5701 tttcaataat tcttcgatca ggttccccag aacctggcct gtgacatcat ccacatttgt
     5761 ctcaagcatt tcgatcatgt ctgaaattag cgcgtcatct atctctcccc tgataattcg
     5821 caatacatta gggacaaaaa gatctttgct tcctgcgcca tagcctattt tttgtgtttt
     5881 catcgcaggg cagaatcctt tctcattcac aaaatgggca agaagcgcag atccggtagg
     5941 cgtgagcaat tctccatcca caggacccat ctgccatggc aggaaatatt cctttaatat
     6001 ctcaagcgca gccggcgccg gtacaggaaa ttttccatgt gagaattcag taaatccttt
     6061 tcccactgat atgggtgcgc agtagatcct ggtatcattt aagccaagat catgaaatgc
     6121 agtacaagct ccaataatat ccgctatcgc atcttcctgc cccagttcat gaaaatgcag
     6181 tttatcgaga gttgtgccat gaattcttgc ttcggctttt ccaaggatgt taaagactga
     6241 aattgcatcc ataataatag caggaggaat atccagagat cttatcctgg caatgatttc
     6301 cggaaaggcc atacagcctt ctttttttat tgatacttcg accgaacagg cccggattcc
     6361 ttttctgttc gatcttgata ttttaacgtc tacattcgca gccagttcca tggcttccct
     6421 ggtttttccg gcatctgctc caagatcaat aagcgcgcct attgtcatat cacccgaagc
     6481 gccgcagaag gggtcaaaga gtattgtatt catcttatcc gacttatacg cttataatga
     6541 tttattttcc cacagctcca aagtaaaatt cgttccatta agaactgatc cattagaaaa
     6601 tggaagtatt gtaattgtcg gtgtttcttc tgttgttggt attatttccg gtgttattgt
     6661 aggtgttgcg ggtgaagagg tagtgggtgc aggcgttgct gctaccgtag gcgctgctgt
     6721 tactgtaggt gttgctgcta ctgtagacgt tgctgttact gtaggtgttg ctgctactgt
     6781 agacgttgct gttactgtag gcgctactgt tactgtaggt gttgctgcta ctgtagaagt
     6841 tgctgttact gtaggcgctg ttgttaccgt aggcgctgct gttattgtag gcgctactgt
     6901 taccgtaggc gttgctgtta ttgtaggtgc tactgttacc gtaggcgttg ctgttattgt
     6961 aggcgcta
//
LOCUS       DATGBN010000283         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_333054, whole genome shotgun sequence.
ACCESSION   DATGBN010000283 DATGBN010000000
VERSION     DATGBN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333054"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1415)
                     /locus_tag="VKL21_06860"
     CDS             complement(105..1415)
                     /locus_tag="VKL21_06860"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HMB45525.1"
                     /translation="MTIGKSVCCESISNHRPAISIPLDRKYDLVCSGCNSAFFEDRLK
                     CDFDNGLLRTNYRNKKLGLKNVPGIGKFHDWLPLDELMTTDSGPVTYRSTELAQYLGL
                     SNLFIGFNGYWPERGAYLKTCSFKELEAFPTLSRFKNSEKSLVLASAGNTARAFAHVS
                     AMTGVDVYIVVPGSGISRMWLPEEPSDSIHFIQMGNNCDYTDSIHLAERLSAFPGMQA
                     EGGARNVARRDGMGTVMLDASVFMKRIPDHYFQAIGSGTGGIAAWEASLRLIEDGRFG
                     TSLPELHLSQNLPFVPMFNAWKAKRRDIVKDIDMPEPKRLIHEMYADILSNREPPYSV
                     PGGVYDALRATEGTMYAISNDDAKNAVNLFEELEGIDIFPPAGVAVASLIEAVDTGKV
                     KKDEHIVLNITGGGVKRVEQDYEICKIKPSAYAENANVPLDELI"
     gene            1680..1934
                     /locus_tag="VKL21_06865"
     CDS             1680..1934
                     /locus_tag="VKL21_06865"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HMB45526.1"
                     /translation="MEKKLKFFFDKQGDMLDISIGEPKGAISKEMDNDIVMRFDPKTN
                     KIVGFTILNFEKRFEHLNKSETLPITATFAQIDKAIEAEG"
     gene            complement(2112..2732)
                     /locus_tag="VKL21_06870"
     CDS             complement(2112..2732)
                     /locus_tag="VKL21_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45527.1"
                     /translation="MPEHSGLRLVALKCKKCGSLLNADNRDVVFYCGNCDSGFELINE
                     KDELVTVDIDFAIPNKKMNAQVVYYPFWVYDANIKIDSRDAAGSITGFITNIFGKEDK
                     KVGKFYVPAFETPLENIRKLGLEFTKSQPEFDVIKKGTLKGCSYSSKDAEKLADFIFL
                     SIEAGKSDMLRNITYSLGLSSPKVIAVPFYKLSGSGMVDGIIGVNI"
     gene            complement(2732..>2983)
                     /locus_tag="VKL21_06875"
     CDS             complement(2732..>2983)
                     /locus_tag="VKL21_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45528.1"
                     /translation="FIGSLMAGNLLLTTSLRYYSADSNDLLIVGAIAGIALIIFGFLK
                     FRYGGEVVEGKKAGLEGMNFRETFNSLNDNGLINIPGVK"
BASE COUNT          878 a          646 c          578 g          881 t
ORIGIN      
        1 tggcagtgtt gctgcgatat tgatcccgtt atgtttaaga atatccacaa ctttctgttc
       61 aggggtatct ctggaaataa gatggtccat gcattgatct gatgttaaat taactcatcc
      121 aggggaacat tcgcattttc ggcatatgca gagggtttaa tcttgcatat ctcataatcc
      181 tgctcaacac gctttactcc cccgccggta atatttaata cgatatgttc atcctttttc
      241 acttttccgg tatcaacggc ttcaattaat gacgcgacag caactccggc aggcgggaag
      301 atatcgatcc cttctagctc ttcaaataga tttacggcat tcttcgcatc atcatttgaa
      361 atcgcataca tggttccttc agttgcccgc aacgcatcat agacgccccc gggtacggaa
      421 tatgggggtt cgcggttgga gaggatgtcg gcatacattt catgaatgag cctcttgggt
      481 tcgggcatat caatatcttt gacgatgtcg cgccttttgg ctttccatgc gttgaacatt
      541 ggcacaaatg gcaggttctg cgaaaggtgt agttccggga gtgatgtacc aaatcttccg
      601 tcttcaatta atcggagcga cgcttcccat gcggctattc ctccggtccc gcttcctatt
      661 gcctggaaat aatgatcagg tatccttttc ataaaaaccg atgcatcaag catcactgtt
      721 cccataccgt ctcttcgtgc aacgttcctt gcgccgcctt cagcctgcat gcctggaaac
      781 gctgatagtc tctcagcaag gtggatggaa tcagtataat cacaattatt acccatttgt
      841 ataaaatgta tggaatccga aggttcctca ggaagccaca tccttgaaat gccgcttccc
      901 ggcaccacaa tataaacatc gacccctgtc attgcagaca catgggcaaa agcccgcgcc
      961 gtatttccgg cagaagcaag gacgagggat ttttcagaat tcttaaatct ggataatgtt
     1021 ggaaaagctt caagttcctt gaaactgcat gtttttagat atgctcctct ttcaggccag
     1081 tagccgttaa aaccaataaa aagattcgaa agccccagat attgcgcaag ctccgtgctc
     1141 ctgtatgtga ctggtccgga atcggttgtc atgagttcat ccagcggcag ccaatcatgg
     1201 aatttcccaa tcccggggac atttttcaat ccaagtttct tgttcctgta atttgttcgc
     1261 aaaagaccat tgtcaaaatc gcatttgagg cggtcttcaa aaaaagcgct gttgcagccg
     1321 gaacaaacaa gatcatattt ccggtctagc ggaattgaaa tcgcaggacg atgatttgaa
     1381 atgctttcac agcacacgga tttgccaatt gtcataatta atgatttcag tcgaggagta
     1441 tatagattta tccattaaat acatcaaatt ggccgaataa ggacagtatt attttttcaa
     1501 aatactattg tacaatgata ctacagtgaa tcaagacagg attgacagga tgaacaggat
     1561 ttatcctgtt attcccgttt gtgtaacata tttcataaca tgtgtgttgt tgttcgatta
     1621 gatgaaaaat cgatagttac agcatatatt actgatagaa taaagagctg cgatgttata
     1681 tggaaaaaaa attaaaattc ttcttcgata agcaagggga tatgctggat atcagtattg
     1741 gtgaacctaa gggagcgatt tctaaagaga tggacaatga tatcgtcatg cgatttgacc
     1801 ctaaaacaaa taaaattgtt ggttttacca ttttaaactt tgaaaagaga tttgagcatc
     1861 tgaacaagtc agaaacgctt cccataaccg ctacttttgc ccagattgac aaggcaatag
     1921 aagcagaagg atagaagtct gtaaaaaaac tgcgtataat ttaggttata tatgcagaaa
     1981 tgagcgtggg atgtctcagc aaaatccatt tgtacaatat gagagccgaa ttcttcaata
     2041 agacgggatt ttaaaatgca tggcttatga aaattaggta catatttaaa aatatatgaa
     2101 taattactaa tttaaatatt gacacctatt atcccgtcca ccatgcctga accgctcaat
     2161 ttgtaaaatg gcactgcgat cactttcggg gatgaaagtc caagcgaata tgtaatattt
     2221 cttaacatat cggattttcc ggcttcaata ctcaggaaaa taaaatcagc taatttctcg
     2281 gcatccttac ttgaatacga acatcctttt agagtgccct tttttattac atcgaactca
     2341 ggctggctct ttgtaaattc aagccctagc ttcctgatat tttcaagggg tgtctcaaaa
     2401 gcgggaacat agaacttacc aaccttctta tcttctttac cgaaaatatt cgtaatgaat
     2461 ccagtgatac tgccggctgc atcccttgag tctatcttta tatttgcatc atatacccag
     2521 aatggataat atacgacctg agcgttcatt ttcttattcg gaatggcaaa atcaatgtcc
     2581 acagtaacca gttcatcctt ttcatttatt agttcaaacc cggaatcgca attgccgcaa
     2641 taaaatacaa cgtctctgtt atccgcattc aaaagcgacc cgcatttctt gcactttaat
     2701 gccacaagtc tcaatcctga atgttccggc attatttcac cccggggata ttgataagac
     2761 cattatcatt taaagaattg aatgtctccc gaaaattcat gccttccaat cctgctttct
     2821 ttccctcgac aacctcaccg ccatacctga atttcaaaaa gccaaatatg atcagtgcga
     2881 tacctgcaat tgcgcccact atgagcaggt cattggaatc ggctgaataa tatcgcaaag
     2941 atgttgtaag gagaagattc cctgccataa ggcttccaat gaa
//
LOCUS       DATGBN010000284         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_410585, whole genome shotgun sequence.
ACCESSION   DATGBN010000284 DATGBN010000000
VERSION     DATGBN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410585"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1049)
                     /locus_tag="VKL21_06880"
     CDS             complement(330..1049)
                     /locus_tag="VKL21_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMB45529.1"
                     /translation="MDKFQKGKPDFARYQKGAVHIRSMRSIRTSNTEIVHIIISWLAI
                     SYAFAILLLWSRSDIKPSGEELFNGIFNPLVISLFTVGISFIVHEMSHKIIAQRFGSW
                     AEFRMSPIMLFLMLILVYEFGILFAAPGAVMIYGGNVGRRENGRISLAGPLSNLLLGM
                     AFFLLLSEQGILYEIGKYGVIINITLALFNMIPFGIFDGRKVWDWNKPVYLGTVIAAL
                     LLLYFFYVDLQLMAPVNRTPF"
     gene            complement(1121..2041)
                     /locus_tag="VKL21_06885"
     CDS             complement(1121..2041)
                     /locus_tag="VKL21_06885"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HMB45530.1"
                     /translation="MKIEKYIPIIGMAFMLLLVQLIAIALSKPLEANDFKAFENPEDT
                     GNVFVWVAIILIFTAFIFLVIKLNKKWMIQAFILITVGWTLAYVFYALFSMFPQFMTP
                     DTNLILTILISAALTVILYKFPEWYVIDIIGVILGAGSASIFGISLDIVPVILLLIVL
                     LVYDYIAVYRTKHMVALAEGVMDLRLPILLVIPKHWNYSFRAEKFDKEDREAFFMGLG
                     DAVMPTLLVVSANFFITETTYYSLPLIGAINIPAIGAILGTFLGFSVLMILVMKGKPQ
                     AGLPFLNCGVILGYVAGSLIAGSSIFGGYF"
     gene            2152..2958
                     /gene="pheA"
                     /locus_tag="VKL21_06890"
     CDS             2152..2958
                     /gene="pheA"
                     /locus_tag="VKL21_06890"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HMB45531.1"
                     /translation="MMIGTLGPKGSYSEKAALQWDDKADLRFYDDIFDTVDALLRKEV
                     ECSIVPIENSLEGSVTLTLDLFLEHQLKITGEVIVQIRHCLLSKGKLKDISVIMSHPQ
                     GLSQCRKYIKNNFKEIEIRSSPSTSLAAKLASESKEIAAIASAESAKMYDLDILNENI
                     QDVNENFTRFIVIGKTTPSPTGNDKTSVIVYLEKNRPGALYEILGEFAGRNIDLTRIE
                     SRPTKKALGDYVFYIDIKGHIEDKIIKETLTKIKGKVGMLKILGSYPAAK"
     gene            3054..3701
                     /locus_tag="VKL21_06895"
     CDS             3054..3701
                     /locus_tag="VKL21_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein/riboflavin kinase"
                     /protein_id="HMB45532.1"
                     /translation="MDIQSLKKLALLGAHQKSLELSSVEFAEHIDSSQQTAARKLKAL
                     EGEKLISRQFLPEGQLISITKSGIDTLRKELSDYQEIFSGNGSVKILSGKLITGLGEG
                     QYYISLDGYRSQFREKLGFDPYPGTLNVKLDAKSIDIRKKITQSIKITGFTDQSRTFG
                     KGTCFRVRISDIEGAVITPERTHYPEDIIEIIAPVNLRNYFDLKDGHNVNVEVIK"
     gene            3698..>4140
                     /locus_tag="VKL21_06900"
     CDS             3698..>4140
                     /locus_tag="VKL21_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HMB45533.1"
                     /translation="MSLEKTIDAIRNGKMILLFDAEDREGETDLVIPAICTTPADVAL
                     MRRDGGGLICVAIYAEAAESLGLPFMSDILKSVSKNGHKSLISLVEKKGDIPYDSRSS
                     FSLWVNHRETFTGITDNDRALTIRKVGETVDKVMKGEKIDFGDEF"
BASE COUNT         1340 a          855 c          896 g         1049 t
ORIGIN      
        1 ataatggcag atataacgca gttcatcata cccatgcttc gtgatgtcag ggatgagttg
       61 cggctcatca atgcgaagtt ggatagattg aataaaaaaa tatgagatca gatttcaaat
      121 gagtcaatag tcagttggta aaaactaatg ctgccttaag aggagcttat cgataataag
      181 gctgccagcg cgaccatacc aacaacgagc ataaccacta tattgatggg caactcgatt
      241 gcccgttcat cttttctaaa tttttttaat gtcaaaaaaa ccaccttttg gcacgaacac
      301 tacaggacaa tatttaagat tttccgctct caaaatggcg ttcgattcac cggggccatc
      361 aactgaaggt caacatagaa gaaatagagt aacaatagag cagctatgac cgttccgaga
      421 tacaccggtt tattccaatc ccatactttc ctgccgtcaa atattccgaa agggatcatg
      481 ttgaaaagcg caagagtgat attgatgatc accccgtact ttccgatctc ataaagaatc
      541 ccctgttcag ataacaggag gaaaaaggcc attccaagaa gaagattgga aagcggccct
      601 gcaagcgaga tgcgcccgtt ctcccggcgc cccacatttc ccccgtagat cataaccgcc
      661 cccggcgctg caaataagat gccaaactcg tatacaagaa tcagcatcaa gaagagcata
      721 atcggactca tcctgaattc agcccaggaa ccgaaccttt gagctattat cttatggctc
      781 atttcatgga ctatgaatga aattccaact gtaaataaag aaataacaag gggattgaat
      841 atcccgttga aaagttcttc accggaaggc ttgatatcgg atcttgacca gagaagcagt
      901 attgcgaatg catatgaaat ggcaagccag gaaataatga tatgaactat ttcagtattg
      961 cttgtccgga tgctgcgcat gctcctgata tgcaccgcac ctttttgata tcttgcaaaa
     1021 tccggtttcc ccttttgaaa cttatccatg tgtatgaatt ggattttcct gcatttaagt
     1081 catagggtca aggggcagag aagtacattc cagggtactc ctaaaaatat cccccaaaaa
     1141 tgcttgagcc tgcgatcagg ctccccgcaa catatcccag gattaccccg cagttcagga
     1201 atggaagacc agcctggggt tttcccttca ttacaagtat cataagaacg gaaaatccca
     1261 ggaatgttcc aagaatggcg cctattgccg ggatatttat ggcgccaatg agcgggagtg
     1321 aataataggt agtttcggtt atgaaaaagt tcgctgaaac aacaagcaat gtaggcatca
     1381 cggcatcccc caggcccatg aagaaggctt cacggtcctc tttatcgaat ttctcagccc
     1441 tgaatgagta attccagtgc ttggggatca caagcaatat gggaagtctc aggtccatca
     1501 cgccttcggc cagcgccacc atgtgttttg tccggtaaac tgctatataa tcgtaaacaa
     1561 gaagcacaat aagtaagaga attaccggta cgatatcaag tgatatcccg aatatcgaag
     1621 cagaacccgc gccaagtatc acgcctatta tgtcaatgac ataccattcg gggaacttat
     1681 acaaaataac agtaagggca gcggagatca gtattgttaa tatcaggttc gtatcgggtg
     1741 tcatgaactg tgggaacatt gaaaaaagcg catagaatac atacgccaga gtccagccta
     1801 cggttatcaa gataaatgcc tggatcatcc atttcttatt taattttatt accaggaata
     1861 tgaatgctgt aaaaataagg attattgcga cccagacaaa aacattgcct gtatcttcag
     1921 gattttcaaa tgccttgaag tcattcgctt caaggggttt gctcaaagca atcgcaataa
     1981 gctgcacaag gagcagcatg aaagccattc cgattatggg tatatatttt tcaatcttca
     2041 aattaatctc cacttataat aagatggata gtttttaaaa gtttgctaaa atatggaaaa
     2101 tgagtacaag agcaacaaac tatttctttt aaccgctaat ataaggagca gatgatgata
     2161 ggaacacttg ggcccaaagg ctcttactcg gaaaaagccg ccctgcaatg ggatgataaa
     2221 gccgatctga gattttacga tgatatattt gatactgttg atgctctttt gcgcaaggaa
     2281 gtcgaatgca gtatcgtgcc tattgaaaat tccctggaag gctctgttac gctcactctt
     2341 gacctcttcc tggaacacca gttaaagatc acaggtgaag taattgtcca gataagacat
     2401 tgtcttctat ctaagggaaa acttaaggac ataagtgtca taatgtcaca tccccaggga
     2461 ttatcccagt gcaggaaata tataaaaaac aatttcaagg aaatagaaat aaggtcttcc
     2521 ccaagcactt cacttgcagc aaaacttgca tccgaatcaa aagaaatagc tgcgatagca
     2581 tccgcggaat ccgcgaagat gtatgacctg gatattctta atgaaaacat ccaggatgta
     2641 aatgagaact tcacccgatt cattgttatt ggcaaaacaa caccatctcc tacaggaaat
     2701 gataaaacat ccgtaatcgt gtatcttgag aaaaacaggc ctggagccct atatgagata
     2761 cttggcgaat tcgcgggcag aaatatcgat ttgaccagga tcgaatcacg cccaactaaa
     2821 aaagccctgg gagattacgt tttttatatc gacatcaaag gacacattga agataagatt
     2881 attaaagaaa cacttacgaa aataaagggc aaggtgggaa tgcttaagat acttggctca
     2941 tatcctgcag caaaataacg gaggctttct tttatttttc ccttcggata aacgtaagtt
     3001 ttataccccc acaaatccat atttgggtag cacctcaaaa atgagtggac tatatggata
     3061 tacaatcttt gaaaaagctt gcgcttcttg gcgcgcatca gaaatccctt gaattatctt
     3121 cggtagaatt tgcagaacac atcgattcaa gccagcaaac agcagctaga aagcttaagg
     3181 ccctggaagg ggaaaagctc atctcacggc agttcctgcc tgaggggcag ttgatctcca
     3241 taacaaagag tgggatcgat acgctgcgta aagaattaag cgattaccag gagatctttt
     3301 cggggaatgg aagtgtcaag atccttagcg gtaagttaat tacaggactt ggggaaggac
     3361 aatattacat ctcgcttgat ggttacagga gtcagttcag ggaaaagctt ggttttgacc
     3421 cgtatcctgg cacattgaat gtcaaacttg atgcaaaaag cattgatatc cggaaaaaaa
     3481 ttacccagag cataaagatc acgggtttta cggaccagag caggactttc ggaaaaggaa
     3541 cgtgtttcag ggtcaggatt tccgatatcg agggcgccgt cataactcct gagcgaaccc
     3601 attatccgga agatattatt gaaataatag cgcccgtcaa tctccggaat tactttgatc
     3661 taaaagacgg tcataatgtt aatgtggaag tgataaaatg agtcttgaga aaacaataga
     3721 tgcaattaga aatggtaaaa tgatacttct tttcgatgca gaggacaggg aaggagaaac
     3781 agaccttgtg atccctgcaa tatgcacaac accagccgat gtcgctctca tgcggcggga
     3841 tggcgggggg ctgatatgcg tcgcaatata tgcagaagct gcagaaagtc tgggattgcc
     3901 tttcatgtcg gatatcttaa aaagcgtctc aaaaaatggt cataaatccc ttatcagtct
     3961 tgtcgaaaaa aaaggagata taccatatga ctcgcgttca tctttctcgt tgtgggttaa
     4021 ccacagggaa acatttacag gtataaccga taatgatcgg gctcttacca tccggaaagt
     4081 gggcgaaaca gtagataagg tcatgaaagg ggaaaaaatt gatttcgggg acgaattcag
//
LOCUS       DATGBN010000285         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_487933, whole genome shotgun sequence.
ACCESSION   DATGBN010000285 DATGBN010000000
VERSION     DATGBN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487933"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VKL21_06905"
     CDS             <1..434
                     /locus_tag="VKL21_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HMB45534.1"
                     /translation="DKIKTAKSEAVKKEIERMKQQEISSANLEVKKAKLNARKEILDT
                     VYEDAKEMISKLPAEKNQKILKSIIEKNQSGNSKIYSRSKDKSTVTEMTKLEFAGEID
                     CIGGIVMENADGSVVLDFKYDMILKNVSEQSLKQVSDILFG"
     gene            443..1462
                     /locus_tag="VKL21_06910"
     CDS             443..1462
                     /locus_tag="VKL21_06910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HMB45535.1"
                     /translation="MGKSQAVKYAYIVARVRGMKGKLIPKEMYPKFLNMDIPEITRFI
                     GESEYKKEVDELGKKYKGTDLFEHALNLNLALTYRKLIEVSQDEAKYIITEYLRYWDI
                     WNIKTILRGKFSGASEDDILEDVVSAGQLRYRDLLEIIKIGTIEGVIAALSKTPYYPA
                     ISSYKGVLADIENNLDKLYYASLIKAGESSKDQFFLKFLRTEIDLKNLKILFRMKRAG
                     MEREEIIKLLIPGGIELNETQLGKLASLPFPEFVRALEELSYWKAIADISGELTSLIN
                     IETRLDKYGLLNITRISYYYPLSILPVLDYIVSKKMEVDNLRIIVRGKETKLPEEIIK
                     AHLVM"
     gene            1463..1762
                     /locus_tag="VKL21_06915"
     CDS             1463..1762
                     /locus_tag="VKL21_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HMB45536.1"
                     /translation="MEIAVVGNSDFVIGFRLAGIRKIFDATSSDIESKIQDVLNDKNV
                     AVLVVHDDDLRMLSPHMQQTLDDSVEPTVIAIGGKGESTNLRDKIKQAVGVDLWK"
     gene            1795..>2534
                     /locus_tag="VKL21_06920"
     CDS             1795..>2534
                     /locus_tag="VKL21_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HMB45537.1"
                     /translation="MKNKGEIYRISGPVVIITGLNTKMYDVVKVGIEGLMGEVIGIEG
                     DKSTVQVYEETSGVRPGEPVENTGMSLSVELGPGLLESIYDGIQRPLPVLKDKMGDFI
                     KRGVSANGLSREKEWDFVPAVKKGDKLKGGDIIGTVQETKNIEHRILVPPTISGTVSE
                     IKKGKFKVDEVICTLADGKTLTMMQKWPVRKPRPVSKKLTPSTPLITGQRILDGLFPI
                     AKGGTAAIPGPFGSGKTVTQQQLAKWSDT"
BASE COUNT          877 a          441 c          594 g          622 t
ORIGIN      
        1 ccgataaaat aaaaacagcc aaatcagagg ctgttaagaa agagatagaa agaatgaaac
       61 agcaggaaat atcgagcgca aatcttgagg tcaaaaaagc aaaactcaat gcccgcaagg
      121 agatccttga tacggtttat gaggatgcca aagaaatgat ctcaaaactg ccggctgaaa
      181 agaaccagaa aatcctgaaa tccattattg aaaagaatca atcgggcaat agtaaaatat
      241 attcgagaag taaggacaaa tcaacagtta cagaaatgac gaagctggaa tttgcaggtg
      301 aaattgattg catcggtggc attgttatgg aaaacgctga tggaagcgta gttctggact
      361 tcaagtatga catgatcctt aaaaatgtca gtgaacaatc cttaaaacag gtttccgaca
      421 tattatttgg gtgacaaaag gaatgggaaa atcacaagct gtcaaatatg catatattgt
      481 ggccagagtt cggggtatga aaggaaaact tatcccgaag gagatgtatc ccaaattcct
      541 taacatggat atacctgaga tcacacgatt cataggggaa tccgagtata agaaagaagt
      601 ggatgaactt ggaaagaaat ataagggtac tgatcttttt gaacatgcat tgaacctgaa
      661 tctggcattg acctaccgca aattgatcga agtatcccag gatgaggcaa aatacataat
      721 aaccgaatat cttcgatact gggatatatg gaatatcaaa accatactgc gtggaaaatt
      781 ttcaggcgca agcgaggatg atatccttga agatgtggtt tctgcgggtc agttaagata
      841 cagggatcta ctggagatca taaagatagg aaccattgag ggagttattg cagcattatc
      901 aaaaacaccc tattatcctg caatttccag ctataaagga gttctggcgg atattgaaaa
      961 taatctggat aagttgtatt atgccagcct gatcaaagcg ggagaaagtt ccaaagatca
     1021 atttttcctg aaattcctca ggaccgaaat agatttgaag aacttgaaaa tcctattcag
     1081 gatgaaacgc gccggaatgg aacgcgagga aatcatcaaa cttttgatcc cgggtggtat
     1141 tgaacttaat gaaacacaac ttgggaaact tgcatctttg ccttttcccg aatttgttcg
     1201 ggcccttgaa gaattatcat actggaaggc aatagcagac ataagtggtg aattgacctc
     1261 ccttatcaat atcgaaacac gacttgataa atatggtctc cttaatatta ccagaatatc
     1321 atattattat ccgctttcaa ttcttccggt ccttgattat attgtgagca aaaaaatgga
     1381 agttgataat ctaagaataa tcgtgcgtgg aaaagaaact aaacttccag aggaaataat
     1441 aaaagcccac ctggtgatgt agatggaaat agcagttgtt ggaaatagtg attttgtcat
     1501 tggatttcga cttgcaggta ttcgaaaaat ttttgatgca acgtcatcag atattgaatc
     1561 aaagatccag gacgtattga atgataaaaa cgtagctgtt cttgttgttc atgatgatga
     1621 tctaagaatg ttatctcccc atatgcagca aacacttgat gactcggttg agccaacagt
     1681 gatagcgatc ggcggaaagg gggagagtac gaacctgaga gataagataa agcaagcggt
     1741 gggtgtggat ctctggaaat aattctaatt aaattaaaat agcaggtgtc acagatgaaa
     1801 aataaaggtg aaatttatag gatatcagga cctgtcgtaa ttattaccgg cttaaatacc
     1861 aagatgtatg atgtggtaaa agtcggaata gaaggtctga tgggtgaggt aataggcatt
     1921 gaaggagata aatccacagt acaggtttat gaagaaacat cgggcgtaag gcccggcgaa
     1981 cccgtagaaa atactggcat gtccctttcg gtggagcttg gtccaggact gcttgagagt
     2041 atatacgatg gtatccagcg tcccctccct gtgttaaagg acaaaatggg tgactttatc
     2101 aagcgcggtg tttctgctaa tggcctgtca cgtgagaaag aatgggattt tgttcctgcg
     2161 gtcaaaaaag gcgataaact caaaggcggg gatataatag ggacagtcca ggaaacaaag
     2221 aacattgagc atagaattct tgtcccgcct acgatttccg gaactgtaag cgaaatcaag
     2281 aaaggaaaat ttaaagttga tgaagtgatc tgcactcttg ctgacggcaa gacactcaca
     2341 atgatgcaaa aatggcctgt aaggaagcca aggccagtaa gcaagaaact gacaccgagc
     2401 actcccctga taacaggcca gaggatcctt gatgggctat tccccattgc aaagggcggg
     2461 accgcagcaa tcccaggtcc gttcggtagc ggaaaaacag taacacagca gcagcttgca
     2521 aaatggagcg atac
//
LOCUS       DATGBN010000286         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_487982, whole genome shotgun sequence.
ACCESSION   DATGBN010000286 DATGBN010000000
VERSION     DATGBN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487982"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VKL21_06925"
     CDS             complement(<1..281)
                     /locus_tag="VKL21_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45538.1"
                     /translation="MNIKKNNEEKRKYDMLQQEAAASFFKNRKTQFLFQLPTVLFFIL
                     VVIAGFFGIQNSNKSLTTISIWVIWWSLLLISLGLAGRVWCFICPFEAIG"
     gene            complement(271..1635)
                     /locus_tag="VKL21_06930"
     CDS             complement(271..1635)
                     /locus_tag="VKL21_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45539.1"
                     /translation="MKKQKQVFLVLIIAIIALNINIHGNAISSQEHTPSLQWFPSLAT
                     DNDTVYIVWADERNGNYTRIMNNIPHKSGDIYISKASFQNGWVFDKNIRINEIKGTTG
                     HGSPSITVDNNNVYAAWEDDRYGFEELYFSYSEKNNIKFKKDLPVVERAGSQVLPSIS
                     VRNGTVYAAMMNKKTWDIEFTEGHSTNSVYWFDKPIRVNDDPAGNWHYAPSLAVDDDK
                     NVCIAWLDARKGNYNIYFSHGTKENGIWRFSTNTRVNYESTNVSHYTPSLAFENGSVY
                     IAWYDDRNKDFDIYLSTGRFENDSWKFDSNMRVNDDEKGDQMHPDIAVKDSEIFIVWE
                     DGRDGGSDIYFSKGVKKNGSLEFIKNTKVNDIKGKHYDPQIGVGGSDVYVAWQAEVND
                     GGDIYFSDGKYDGKTWVFSRNTKINDDLTDSFTKSDPAFLGLILGPVIVSVIIVGFLR
                     KYEH"
     gene            complement(1632..>2192)
                     /locus_tag="VKL21_06935"
     CDS             complement(1632..>2192)
                     /locus_tag="VKL21_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMB45540.1"
                     /translation="NSECGSTTTYVKETRTFFYSCLDIDCGTATLTILIKKVHPEADV
                     TGLDGDPEILQMAGKKVTKASLDIKLDLGMSFELPYPDHSFDRVVSSLVFHHLTRENK
                     ALTIKEIFRVLSPGGELHVADFGKPQNALMYLISLVLRHLEETRDNINGLLPEMFWKV
                     GFEQVEETARFMTLFGTLSLYRARKP"
BASE COUNT          585 a          483 c          410 g          714 t
ORIGIN      
        1 ccgatggctt caaaaggaca tataaaacac cagacccttc ccgctagccc caggcttatt
       61 aaaagaagcg accaccatat aacccagatt gaaatggttg taaggctttt gttgctgttt
      121 tgaatcccga aaaaacctgc aattacgaca agaatgaaaa atagaactgt tggaagttga
      181 aagaggaact gggttttgcg gtttttgaaa aaagatgctg cagcctcctg ttgaagcata
      241 tcatattttc ttttttcctc attatttttt ttaatgttca tattttctta agaatcccac
      301 gattatgacc gatacgataa ccggaccaag aatcaagccc aagaatgctg gatccgattt
      361 tgtaaacgaa tctgttaagt catcgtttat tttggtattc ctgctgaata cccaggtttt
      421 accatcgtat tttccatctg aaaaatatat gtctccgccg tcatttactt ctgcctgcca
      481 tgcaacataa acatcgcttc ctcctacccc gatttgaggg tcgtaatgct ttcctttgat
      541 gtcattaact tttgtgtttt ttataaattc aaggcttcca ttcttcttta caccttttga
      601 gaagtaaatg tcagagccgc cgtccctgcc atcttcccat actatgaaga tttcactatc
      661 cttgacagca atatcaggat gcatttgatc gcctttctca tcatcattta cccgcatgtt
      721 actgtcaaat ttccagctgt cattttcgaa cctgcctgtt gataagtata tatcaaaatc
      781 tttattcctg tcatcgtacc atgcaatata cacacttcca ttctcaaatg ccagtgaagg
      841 agtataatgg gatacatttg tggattcata attcaccctc gtgttcgtgc taaatcgcca
      901 gatcccgttt tcttttgttc catgtgagaa ataaatatta taattgcctt tcctcgcatc
      961 cagccaggca atacacacat ttttgtcatc atccacagca agcgatgggg cataatgcca
     1021 gttaccagca gggtcgtcat tcactcttat cggtttatcg aaccagtaaa cactgttggt
     1081 agaatgccct tctgtgaatt ctatatccca ggttttcttg ttcatcattg ctgcatatac
     1141 ggttccattg cggactgaaa tggagggcag cacctggctt cctgctcttt ctacgaccgg
     1201 taaatccttt ttaaacttaa tattgttttt ttcggaatat gaaaaataaa gctcttcaaa
     1261 tccatatctg tcatcctccc atgctgcata gacattgttg ttatcaacgg ttatcgaggg
     1321 agagccgtgc ccggtagttc ctttaatttc atttatccgg atattcttat caaaaaccca
     1381 gccgttttga aaactcgctt ttgaaatata tatgtccccc gatttatggg gtatattgtt
     1441 cattatcctt gtgtaattgc catttctctc atcagcccag acaatgtata cggtgtcatt
     1501 atcagtagct agagaaggga accactggag ggaaggggta tgttcttgag aagagatggc
     1561 atttccatga atattgatgt tcaaagctat aattgctatt atgagaacaa ggaatacttg
     1621 tttttgtttt ttcatggttt tcttgccctg tacagggaaa gtgttccaaa aagcgtcatg
     1681 aatcttgcgg tctcttctac ctgttcaaat ccaaccttcc agaacatctc aggcagaagg
     1741 ccattaatat tgtctcttgt ttcctcaaga tgccgcaata ccagggatat caaatacatt
     1801 agcgcattct ggggtttccc gaagtctgca acatgcagtt ctcctcctgg cgagaggact
     1861 ctgaatattt ccttaattgt gagggctttg ttttccctgg tcagatgatg aaaaaccagg
     1921 cttgaaacta cacggtcaaa agagtggtca ggatacggca gttcaaatga catccccagg
     1981 tcaagtttaa tgtcaagact tgcttttgtt acttttttcc ctgccatctg aaggatttca
     2041 gggtctccat caagcccggt tacatcagct tcaggatgga ctttctttat gagaatagtg
     2101 agtgtggcag taccacagtc tatgtctagg caagaataga aaaaggttct cgtctccttg
     2161 acataggttg tggtactgcc acactcacta tt
//
LOCUS       DATGBN010000287         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_333197, whole genome shotgun sequence.
ACCESSION   DATGBN010000287 DATGBN010000000
VERSION     DATGBN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333197"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VKL21_06940"
     CDS             <1..633
                     /locus_tag="VKL21_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HMB45541.1"
                     /translation="TGGMGSYNDSRDILPFMKESDYSDAKKIMGDTVRAIKKETRVAY
                     QGILYGQFMATAKGISVIEFNARFGDPEAMNVLPLLENDFLDVCRNIVNGTLDKINVQ
                     FKKQATVCKYAVPAGYPDDPVKDSIVEIGNMKDSLLFYSSVYEKDNKIYTTGSRALAV
                     LGISDTIADAEKKARAGLAGLKGMLHSRRDIGMDELIRKRVQHMKELRAN"
     gene            652..1026
                     /locus_tag="VKL21_06945"
     CDS             652..1026
                     /locus_tag="VKL21_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45542.1"
                     /translation="MDNDTTNKQPEKQKDKSLENLERMLDKLPENARKPILDLIDKRK
                     VELGLLKSELKPLGYKLKKGSAKPKPKPPTKENMESLRKIAGNIGRIKETTETNFHDV
                     DVKDEPLKKEKKALEEVDSYKY"
     gene            1036..1317
                     /gene="gatC"
                     /locus_tag="VKL21_06950"
     CDS             1036..1317
                     /gene="gatC"
                     /locus_tag="VKL21_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HMB45543.1"
                     /translation="MIGKKDLEHIGWFARLELSEEDKEKYTPKLNSILDYFGEIDKAD
                     TEGVEPLYHVLPMSNVFREDVPTRSLSQEEALSNAPKNQDGFIKAPRMM"
     gene            1318..>2161
                     /locus_tag="VKL21_06955"
     CDS             1318..>2161
                     /locus_tag="VKL21_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HMB45544.1"
                     /translation="MASINELRERIHADSSGEIVAEYLERITKSKLNAFITVSKESAL
                     SQAQEIDAKGHNGPLAGIPIAIKDNISTKGISTTCASKILTGYIPPYDAHVIEKLKNA
                     GAIIIGKTNMDEFAMGSSTETSFYGPTLNPWDTTRVPGGSSGGSAAAVSGGEAPLSLG
                     SDTGGSVRCPASFCGVVGLKPTYGVVSRYGLISYANSLEQIGPFASSVQDVATLFDVI
                     AGHDPRDSTSVKRAGNYSSALENDVNRLKIGIPEEYFGEGIDRNVEKSVRDAINTLEE
                     LGATS"
BASE COUNT          699 a          413 c          537 g          512 t
ORIGIN      
        1 accgggggta tgggttcata caatgattca agagatattt tgccattcat gaaagaatcc
       61 gattacagtg acgccaagaa aataatgggt gatacggtaa gggcaataaa gaaagaaacc
      121 cgtgttgcgt accagggcat cctgtacgga caattcatgg cgaccgcaaa aggtatttca
      181 gtcatagaat tcaatgcgcg ttttggggac ccggaagcga tgaatgttct tcctcttctt
      241 gaaaatgatt ttcttgatgt ctgccgaaac attgtcaacg ggacacttga caagatcaat
      301 gtgcaattta agaaacaggc aactgtatgc aagtatgcag ttccggcagg atatcctgat
      361 gatcctgtaa aagattccat tgttgaaatt ggaaatatga aggattctct tctcttctat
      421 tcaagcgtat atgaaaagga taataagatc tatacgactg gttccagggc tcttgctgtt
      481 cttggaattt cagatactat cgcggatgcg gaaaagaaag cccgggcagg actggcaggt
      541 ctgaaaggaa tgttgcacag caggcgggat atcggcatgg atgaactcat ccggaagagg
      601 gtacagcata tgaaggaatt aagggctaac tgaacattat atattataat tatggataac
      661 gatactacaa ataaacaacc ggaaaaacaa aaggataaat cccttgaaaa tcttgagcgg
      721 atgcttgata aattgcctga aaacgccagg aagccaatac ttgatctgat agacaagcga
      781 aaggttgaac ttggactctt aaaatcggaa ttaaaaccac tgggttataa gttgaaaaag
      841 ggaagtgcca aaccaaaacc taaaccgccg accaaagaaa acatggaatc ccttcgaaaa
      901 atcgcgggga atattggcag gataaaagaa acaacggaaa cgaattttca tgatgtggat
      961 gtcaaagatg aacctctaaa gaaagagaaa aaagcattgg aagaggttga ttcatacaaa
     1021 tattgaggaa gcattatgat tggaaaaaaa gatcttgagc atatagggtg gtttgcgcgc
     1081 cttgagttaa gtgaagagga caaggaaaag tacactccaa agctcaactc gattcttgat
     1141 tatttcgggg agatagataa agcggatacc gaaggtgtgg aacctttata tcatgttttg
     1201 cccatgagca atgttttcag ggaagatgtc ccgacaagat cattgtcgca ggaagaagcg
     1261 ctttcaaatg cccctaagaa ccaggatgga ttcataaaag ccccgcggat gatgtaaatg
     1321 gcatcgataa acgagctcag ggaaagaatt catgcagatt cctccgggga aattgttgcc
     1381 gaatacctgg aaaggataac aaagagcaaa ttgaatgctt ttatcactgt atcaaaagaa
     1441 agcgcacttt cacaggcaca ggaaatagat gcaaaaggac acaatggacc gcttgccgga
     1501 ataccaatag caataaagga caatatatca acaaaaggca tcagtaccac atgcgcctca
     1561 aagatattga caggttatat tcctccctat gatgcccacg ttattgaaaa actcaaaaat
     1621 gcgggtgcta tcattatcgg taaaaccaac atggatgaat tcgcaatggg ctcatcaaca
     1681 gagaccagct tctacggccc tacacttaat ccctgggata cgaccagggt tcccgggggc
     1741 tcgtcaggcg gaagtgcagc agcagtgtca gggggcgaag cgccgttgtc ccttggttct
     1801 gataccggcg gttcagtgcg ctgccccgca tcgttttgcg gtgtggtggg gctgaaaccc
     1861 acgtatggtg ttgtatctcg ttacgggctt atatcctatg cgaattcgct tgagcagata
     1921 ggaccgttcg catcctcagt tcaggatgtt gccactcttt ttgatgtaat tgcaggtcac
     1981 gatcccaggg attccacatc tgtgaaaagg gcgggaaact attcctcggc gcttgagaat
     2041 gacgttaata gattgaagat cgggattccg gaagaatatt tcggggaagg tattgatcgg
     2101 aatgtagaaa agtcggtaag ggatgccatc aatacacttg aggaactcgg ggcgaccagt
     2161 a
//
LOCUS       DATGBN010000288         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_449818, whole genome shotgun sequence.
ACCESSION   DATGBN010000288 DATGBN010000000
VERSION     DATGBN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449818"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..76)
                     /locus_tag="VKL21_06960"
     tRNA            complement(2..76)
                     /locus_tag="VKL21_06960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Pro,seq:ggg)
     gene            complement(111..941)
                     /gene="nadC"
                     /locus_tag="VKL21_06965"
     CDS             complement(111..941)
                     /gene="nadC"
                     /locus_tag="VKL21_06965"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMB45545.1"
                     /translation="MLLTELERFIEEDLGYDDVSCNMIPDCRVQAEVIVKEFGIIAGL
                     FEATQIFEYFEIFPTTDFEEGDMVKENEIIFNLDGGARSILKAERLALNFLGRMSGIA
                     TLTRQFVERAAGPGIAGTRKTTPGFRKFEKKGILAGGGDPHRFNLSDAVMIKDNHIAV
                     LGLEKAIISAKKAASFTRKIEVEVETIDDAVHAAKMGVDIIMFDNMTVNEIKQAIKIL
                     KEKELRDNIILEASGGISIENVDKYGKTGVDVISIGALTHSSNWLDISLRIIPNITKE
                     "
     gene            1019..1831
                     /locus_tag="VKL21_06970"
     CDS             1019..1831
                     /locus_tag="VKL21_06970"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HMB45546.1"
                     /translation="MLKIGVIGCGAIGAQICKAIDNGRISAQLVAIYDRSSEHCESLL
                     RSLKNKPIMSSPAELISKADIVVECASQEAVRELGLSVLEEGKDLMVMSVGALMEQEL
                     LCQFILAARKNNCRIYIPSGAIAGIDGLKSASMACIDKVILSTVKNPGGLSGAPFIIE
                     NNIDPGSFNKKTLIFEGNAREAVRAFPANVNVAASLSLAGIGAEKTLVKIFVDPGASR
                     NIHEITVIGDFGTFTSRIENIPSPDNPRTSLLAALSAIATLKKITEPLQLGT"
BASE COUNT          531 a          430 c          366 g          576 t
ORIGIN      
        1 atggggtcgc tgagatttga actcaggtct catgcacccc aagcatgaag gatggaccag
       61 gctaccctac gaccccgcag gtgcactgcc ttagatatcc tcagggtatt ttattctttc
      121 gtaatatttg ggatgattcg aaggcttata tcaagccagt ttgatgaatg tgtgagcgct
      181 ccgatcgaaa taacatccac ccccgtcttt ccatatttgt caacgttttc gatagaaatt
      241 ccgcctgacg cctcaagtat tatattatcc ctgagttctt tttctttcag tatttttatg
      301 gcttgtttta tttcattcac ggtcatatta tcgaacatta ttatatccac acccattttt
      361 gccgcgtgga ccgcatcatc tattgtttct acctcaactt ctattttcct ggtaaaactt
      421 gccgcttttt ttgcggaaat tatcgctttt tcaagtccaa ggacagctat atggttatct
      481 tttatcataa cagcatctga tagattgaac ctgtgagggt ctcccccacc tgccaggatc
      541 cctttttttt cgaattttcg aaatccaggc gttgttttcc ttgtacctgc tatccctggc
      601 cctgccgctc tttctacaaa ctggcgtgta agtgttgcta tcccgctcat tctccccaga
      661 aaattcaatg caagcctttc tgccttgagg atcgatcttg ctcccccatc caggttgaaa
      721 atgatctcat tttcctttac catatctccc tcctcaaaat ccgtagtcgg gaatatctca
      781 aaatattcaa atatctgggt ggcttcaaaa agccctgcaa taattccaaa ttctttaacg
      841 atcacttctg cctgcaccct gcaatccgga atcatattgc atgaaacatc atcataaccg
      901 agatcttctt caataaatcg ttccaattca gtcaataaca taatcaaatc cctctaatta
      961 attatctata aattacttat atgctactac taaatatagg aaagtgaatc agtaatatat
     1021 gctgaaaatc ggagttatcg gctgcggtgc aataggagcc cagatttgta aagcgataga
     1081 caacggaaga ataagcgcac agcttgttgc tatttatgac agaagcagcg agcattgtga
     1141 aagtctcctc cggtcactca agaataaacc catcatgtct tcaccagcgg aattaatatc
     1201 aaaagcagat atcgttgtgg aatgcgcttc ccaggaggct gtgcgcgaac ttggtctttc
     1261 tgttcttgaa gaaggaaaag acctcatggt tatgagcgtg ggcgctctta tggaacagga
     1321 gttgttgtgt caatttattt tggccgccag gaaaaataat tgtcggatat atataccatc
     1381 gggggccata gcaggcatag acggactaaa atcggcatct atggcctgta ttgataaagt
     1441 aatcctatca actgtgaaaa atccgggggg attaagcggc gcacctttta taatagaaaa
     1501 taatattgat cccggctctt ttaataaaaa gaccctgata ttcgaaggaa acgcaagaga
     1561 agctgtccgc gcatttccgg caaatgtcaa tgttgctgcc tcgttgagcc ttgccggcat
     1621 cggggctgaa aaaacactgg tgaaaatttt tgttgatcct ggagcttcaa ggaacattca
     1681 tgaaattaca gtgatcgggg attttggcac attcacatcc cgcattgaaa atatcccgtc
     1741 tcccgacaat ccaagaacaa gtcttcttgc agcgctatcg gctattgcaa cattgaaaaa
     1801 gataacagaa ccgttacagc tcgggacttg aaatgtcttt tgaagggatc ttttcaataa
     1861 tttccgaatt gaaaagaaaa atattatata ttatcgcact ctt
//
LOCUS       DATGBN010000289         8811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_527392, whole genome shotgun sequence.
ACCESSION   DATGBN010000289 DATGBN010000000
VERSION     DATGBN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527392"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..551
                     /locus_tag="VKL21_06975"
     CDS             372..551
                     /locus_tag="VKL21_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HMB45547.1"
                     /translation="MKSSTQDKVESNVHKVKGGVKEIAGKLSKNTNLEIEGKEEKIEG
                     KVQEKVGQIKKVFGK"
     gene            1304..1510
                     /locus_tag="VKL21_06980"
     CDS             1304..1510
                     /locus_tag="VKL21_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45548.1"
                     /translation="MELKEINVPEGIVLQIDHEGQFAGITVDVGKGNNIYSNVKAEEI
                     NKERARYLQNTDEMLEEWHSCTQA"
     gene            1598..2719
                     /locus_tag="VKL21_06985"
     CDS             1598..2719
                     /locus_tag="VKL21_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45549.1"
                     /translation="MVKKSEERRDIHTEDINTENKDSFKTCEDSYTAVSKMWEDSYLK
                     LYKPWFESTEALFGKAFELSKGAAPETYKEFYGEWVKTSQNSFGKFYQIPALESNKET
                     FDKLLVSAEESKKIYSSWIAELDENSRATREVLKCEADPAKYKEVYDMWINSYGKMLN
                     ELLTLPLRQNMKEIFENITGTPDIYSEAFEQIATIWKDSYAKLYVPYAESLLKLSMKS
                     AEISRGNASPEAYKEFHTVWMNTYQEIYGRLFDTRSMRPSKEVLERFERSANVNLNLY
                     KSWIGTLGKLSQKAEELSEHCAEPEAYKDFYNLWAKMYGKAFDNFFENTPTFSPFKEI
                     MEPVKNAARIYADTFGNISNMWVKPGVSSASAYPGKYTT"
     gene            2740..3288
                     /locus_tag="VKL21_06990"
     CDS             2740..3288
                     /locus_tag="VKL21_06990"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HMB45550.1"
                     /translation="MEKSSFEPTQEFLDLWLKTYKATTGRLVEMPAVGPAREKSEKFM
                     KGFSTFVNLYTAGVDTSSNLQSVFMEAMRKVQEKTAKDMEGEISPEKYKEFYNIWIDT
                     YSETFKDFSKSGHFASDMGKLNSHLMDFQKYNREMLEENYLKPMNIPTKTEIDEINKE
                     LYSLKKAIKELNSHIKALEEKK"
     gene            3310..4494
                     /gene="phaC"
                     /locus_tag="VKL21_06995"
     CDS             3310..4494
                     /gene="phaC"
                     /locus_tag="VKL21_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HMB45551.1"
                     /translation="MNGLDLIGNFDKLKYGMELFLKPPEFLVGTTPSEVIFSEDKMKL
                     IHYIPNVEKTHSIPILIVYALVNRYYILDLQPDKSVIKKLLEEGFDVYVIDWGYPSGA
                     DRYLTLDDYVNGYLNNAVDKIMERSGLDNITLLGVCQGGTFSVMYAALHPDKVKNLVT
                     LVAPVDFDTDKGILNLWAKSLDVDKIVDYYGIVPGDFLNSGFLMLDPFRLMIDKYVGM
                     FDRIECKPDDTTSKLRNEDYIKNFLRMEKWIFDSPDQAGETFRQFMKDCYQKNLLIKN
                     EMMLNGKKINLKNINMPLLNVMAEFDHLVPTDASKPLSDAVSSSDKETLVFPTGHIGI
                     FVGSKSQKEVCPKIAAWLKPRSLLNGKEKIESGKVELPESAKEKMNEQRKMARRARVN
                     KK"
     gene            4491..5228
                     /gene="fabG"
                     /locus_tag="VKL21_07000"
     CDS             4491..5228
                     /gene="fabG"
                     /locus_tag="VKL21_07000"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMB45552.1"
                     /translation="MRLENKVVIITGAGSGIGKESALLFAKEGAKVVVADVNEKVGEE
                     TVGEIKKNGDGFFVKLDVSNREQSKQMVKTTLEKYGKIDVLINNAGIVQDAFVSKMTE
                     EQWDKVININLKGVFNCIQAVVEVMMNQGAGVIINTSSIVGLNGNVGQVNYAATKAGL
                     IGMTKTLAKELGRKGIRVNAVAPGFIATPMTSNVPEKILEMMKEKTPLRRLGEAKDIA
                     NAYLYLASDDANFVNGAVLCVDGGLII"
     gene            5411..6262
                     /locus_tag="VKL21_07005"
     CDS             5411..6262
                     /locus_tag="VKL21_07005"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HMB45553.1"
                     /translation="MGKKAGLEGKVALVTGGSRGIGQAIALRLAKDGADVAINYQSTK
                     EQAEKLSKLIDQMGMMDEFDRLSLKIDLMETKENAKEVSELIDSMGKHSIICQANVND
                     FEQVNRMRDEVVKQFGRIDILVNNAGIVRDKSFVKMTPQMWTEVLSVNLDGAFYCTKA
                     VIEGMLERKYGRIVNISSVIGRMGNRGQANYAASKAGLIALSQTLAKEFAGKGITVNA
                     VAPGFIETEMLRSVPKEIMDKILAQIPLGRLGKPAEVAGAVAYLVSEDGEYMTGQVID
                     INGGLYI"
     gene            6343..6415
                     /locus_tag="VKL21_07010"
     tRNA            6343..6415
                     /locus_tag="VKL21_07010"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6377..6379,aa:Asp,seq:gtc)
     gene            6617..7336
                     /gene="purC"
                     /locus_tag="VKL21_07015"
     CDS             6617..7336
                     /gene="purC"
                     /locus_tag="VKL21_07015"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMB45554.1"
                     /translation="MKLSELYSGKAKTVFKTDDPEKLIMEFRDSLTAFDGKKKSEAPK
                     KGYYNAQIAAKLLTLLEEKGIKTHYDSMMSDTEMVVDAVEIIKIEVIVRNIAAGSLVK
                     KYPFKAGQKIDPPILVFDYKSDEHGDPMINDDIALALGLATRPELLEIRKLALLINSI
                     LVDYLGDKNILLPDFKLEFGRRKGKIVLADEISCDTCRFWDKATGESLDKDVFRFDKG
                     DLVAAYREVARRVVPEIFKST"
     gene            7333..8040
                     /locus_tag="VKL21_07020"
     CDS             7333..8040
                     /locus_tag="VKL21_07020"
                     /EC_number="1.5.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromethanopterin reductase (acceptor)"
                     /protein_id="HMB45555.1"
                     /translation="MRVAWCITGAGHFLCESFEVFKELREKNKELKVTSFVSRAAEEV
                     IKMYGLQNDLLNISSGGYLEECFYEREQGSSFPKAGRFLLNKYDALVVTPATSNTIAK
                     LAYGIADTLVTNAVAQAVKGNVPVYIVPVDIEGYIESKMPFFIEREICMKCEVCPPMK
                     DCPNKAITDQIDLLKCNGCGICVKLCSYGAIRGEITKIKVRDVDSKNVKILSELEGIT
                     ILNRPARALDLIEQCKN"
     gene            8091..8315
                     /locus_tag="VKL21_07025"
     CDS             8091..8315
                     /locus_tag="VKL21_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HMB45556.1"
                     /translation="MFPTKKVQTLIGSIVQVEMKGDKHTLEGKLESADEYLNLHLNDT
                     YEIADGVKSRSLGSVILRGNNIIMLSPIKQ"
     gene            8325..8663
                     /locus_tag="VKL21_07030"
     CDS             8325..8663
                     /locus_tag="VKL21_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMB45557.1"
                     /translation="MDIEEKILKLIKSKKNGILQNELWKTAKIDSSKCSRIVAKLEKD
                     RLITREQNSSKGTKTFLIRSVTKKEEKAKNFKLLLSKEVFSPCTGCSLECVPENCMPL
                     SEWVHGLQNE"
BASE COUNT         2963 a         1517 c         2039 g         2292 t
ORIGIN      
        1 gtaaaaatat tagaggcaaa agctgatgta cacccattaa ttgttattgc aggaatcata
       61 ggaggagtga atttgttcgg cgttgtaggt ttatttttag gcccgatact tctgcctttg
      121 ctcataacat attttgaaac cttcagagaa agatttgaat gaaatgggat tggacgcagt
      181 ggaaatgagc aaaaaagtat tatgttgtca gtctttcata caatatcctt taaatagtat
      241 acattcatat tagataatat cgagtggcgg gcataacgga aggtaataac cgataattcc
      301 atgaattcat cggcttaaga cacctgaaag gatgcaatag ccataagata ttatacaaac
      361 ggaggaatac tatgaaaagc agtacacaag acaaggtaga aagcaatgtc cacaaagtga
      421 agggtggggt taaggaaatt gcggggaaac tcagcaagaa taccaatctt gaaatagaag
      481 gcaaagaaga aaagatagaa ggcaaagttc aagaaaaggt gggccagatt aagaaggtct
      541 ttgggaagtg agtaggttag attaatggga ttattagaga caatagtcgt aattctggta
      601 atcctctggc tattgggata ttcgctccat ttagctgggg ggttaatcca tatattgctg
      661 gttattgcta ttatcgttat attgattaga ataatccagg gacgcaaacc tttgtaaccg
      721 ttccggattt tttttacaat tttctgtatt gagcgcccat tttttaagat actaatcaac
      781 ttgatgaata tctaaacgca acactgaacc atcgcacttt ggatgatttt tgcaaaccca
      841 cctctcccgt attgcatccc actttatttc tttttcctta taggtttcac cacaatgttc
      901 acatcgtact ttcttttctg attctaaatc cggtctaaaa ggaggttttg tctcatcatt
      961 gcgagctttc atatcctcgg attgcttact tccaggtatc atagtgcctg taagatgatt
     1021 cttcaaccat aaaaaggtag tgaaacctgc atctagcttt ttaatattat gtttgtaaat
     1081 tcaatgattg atcccaaata caatgattaa tcataaatac aattattaat attaaaaaaa
     1141 attatatatg aatgtataac tatatctgtg gtggtgacta ttaaaacaat tccagatatg
     1201 ataggttgtg gggttgtgat gagacctggc agcaatagga acaactgggt tcaggaataa
     1261 tacccttgaa ttacttcagg ggactaatta aaaggtgaaa taaatggaat tgaaagaaat
     1321 aaatgtaccg gaaggtatcg tcctgcaaat tgatcacgaa ggacagtttg ccggtattac
     1381 agttgatgtt ggaaaaggaa ataatatcta ttccaatgtc aaggctgaag agataaacaa
     1441 ggaaagagcg agatacctcc aaaatactga cgaaatgtta gaagaatggc attcatgcac
     1501 acaggcttga tataaaaaag ttgttgggaa tgagatttgt tcccaacaga ttagttgaga
     1561 ataaaataga ttaaaaaatt aaaagaagtg aataaatatg gttaagaaat cagaagaaag
     1621 aagagatata cacacagaag atatcaacac agaaaacaag gatagtttca agacatgcga
     1681 agatagctac acagcagttt caaaaatgtg ggaagattcc tatttaaagc tatataagcc
     1741 ctggttcgaa tccacagaag cgcttttcgg gaaggcgttc gaactatcaa agggtgctgc
     1801 gccagaaaca tataaggaat tttatggtga atgggtgaaa acctcccaga acagttttgg
     1861 taaattttat caaatccctg cactggaatc caataaggaa acctttgata aactcctggt
     1921 tagcgcagag gaatccaaaa agatctacag ttcatggatt gccgaattag atgagaactc
     1981 cagggctacc cgcgaagtac tcaagtgtga agcagatcct gcaaaatata aagaagttta
     2041 tgatatgtgg atcaattcgt atggaaaaat gcttaacgag cttcttacac tgccattgcg
     2101 gcaaaatatg aaggagatct ttgagaacat cacaggcaca cctgatatat attcagaggc
     2161 atttgagcag atagctacaa tatggaaaga ttcatacgcg aagctatatg taccgtatgc
     2221 tgaatccttg ctgaagctat caatgaaatc agcagagatc tcccggggga atgcgagccc
     2281 tgaagcctat aaggaattcc ataccgtatg gatgaatacg tatcaggaga tatatggccg
     2341 gttgtttgat acccggtcaa tgagaccttc aaaggaagta ttagagcgct ttgagcggag
     2401 tgcaaacgtt aacctgaacc tctacaagtc ctggatagga acccttggaa aactgtcaca
     2461 gaaggctgag gaactatcag aacactgcgc agaaccagag gcatataaag atttttataa
     2521 tctctgggca aaaatgtatg gaaaggcatt tgataacttc tttgagaaca cacccacatt
     2581 cagcccgttt aaggagataa tggagcctgt gaagaatgct gccaggatat acgcagatac
     2641 atttggcaac atatccaata tgtgggtaaa accaggtgtc agttcagcat cagcgtatcc
     2701 gggaaagtat acaacatgag cgattcaggc gggttaagta tggaaaaatc cagttttgaa
     2761 cccacccagg aatttttaga cttgtggtta aagacataca aagccaccac aggcaggctt
     2821 gtcgagatgc ctgctgtggg tccggcacgt gagaaatccg aaaaatttat gaagggtttc
     2881 tctacctttg ttaaccttta tacagcaggg gtggacacca gttctaattt acaaagtgtg
     2941 ttcatggaag caatgcgcaa agtgcaggaa aagacagcaa aagatatgga aggtgaaatc
     3001 agtcctgaga aatataaaga attttataat atctggatcg atacgtatag cgagaccttt
     3061 aaggactttt cgaaatcagg ccattttgca tcggacatgg ggaaattgaa ttcacattta
     3121 atggattttc aaaaatacaa cagggaaatg cttgaagaga attatctcaa acccatgaat
     3181 atccctacaa agacggaaat cgacgagata aataaagagt tgtattctct taaaaaagca
     3241 ataaaggaac tgaatagcca tatcaaagcc ctggaagaga aaaaataagg ttatgggtaa
     3301 ggagatgcca tgaacggact agatttaatt ggaaattttg ataaattaaa atatggaatg
     3361 gaattattcc tgaaaccacc cgaatttttg gttggaacta cgccttctga ggtaatattt
     3421 agtgaggata agatgaaact tatccattat attccaaatg tggaaaaaac acattccatt
     3481 ccaatattaa tagtgtatgc actcgtgaac aggtattaca ttctggatct ccaacctgat
     3541 aagagtgtaa taaagaagct tttggaagaa ggttttgatg tttatgtcat tgactgggga
     3601 tatccgtcag gggcagacag atatctcaca cttgatgatt atgtgaacgg ttatttaaat
     3661 aatgctgtgg ataagatcat ggaacggtcc gggttggata atatcaccct gctcggcgta
     3721 tgtcagggcg ggactttttc tgtgatgtat gccgcattgc accctgataa ggtaaaaaac
     3781 ctggtgactc tggtggctcc tgtggacttc gatactgata aaggaattct taatctatgg
     3841 gcaaaaagct tagacgtaga taagatcgtg gactactatg gaatagtgcc cggggatttc
     3901 ctgaattcag ggttcttgat gcttgaccca ttcaggctta tgatcgacaa gtatgtaggt
     3961 atgttcgacc ggatcgagtg caaaccggat gatacaacct caaagcttcg aaatgaagat
     4021 tatatcaaga atttccttag aatggaaaaa tggatatttg acagccctga ccaggctgga
     4081 gaaaccttca ggcagttcat gaaagactgc taccagaaga atcttcttat aaaaaatgag
     4141 atgatgctaa atggtaagaa aatcaatttg aaaaatataa atatgccttt gcttaatgta
     4201 atggcagaat tcgaccacct cgttcctacc gatgcaagta agccgttgtc agatgcggta
     4261 tccagttctg acaaagagac actggtcttt ccgacgggcc atataggaat atttgtgggc
     4321 tcaaaatccc agaaggaggt ctgtcctaag atcgcagcat ggttgaagcc caggtctctt
     4381 ctcaatggaa aagaaaagat tgaatcagga aaggtagagt tgcctgaatc agctaaggaa
     4441 aaaatgaatg aacaaagaaa aatggctcgc agagcaaggg tgaacaaaaa atgagacttg
     4501 aaaacaaggt cgtaattatt acaggagccg gaagcggtat tggcaaagag tctgcgctcc
     4561 tgtttgcaaa agaaggagct aaagtcgttg tcgccgatgt gaatgaaaag gtcggtgagg
     4621 agacagttgg agagatcaag aaaaatggag atggattttt cgttaaattg gatgtatcca
     4681 acagggaaca atcgaaacag atggttaaaa ctactcttga aaaatatggt aaaatagacg
     4741 ttttgataaa taatgcagga attgtccagg atgccttcgt atcaaaaatg actgaggaac
     4801 agtgggataa ggttattaac atcaatctta aaggagtttt taactgcatc caggctgtag
     4861 ttgaagtcat gatgaaccag ggagccggcg taattatcaa tacatcttct atcgtaggac
     4921 ttaacggtaa tgtgggccag gtcaattatg ctgcaaccaa agccggtctc atcggtatga
     4981 caaaaaccct ggcaaaggaa ctgggaagaa aagggatacg ggtcaatgca gttgcgccag
     5041 gatttattgc cactcctatg acctcaaatg tacctgaaaa aatccttgaa atgatgaaag
     5101 aaaaaactcc attgcgcagg cttggcgaag caaaagatat agcgaatgca tatttgtatc
     5161 ttgcttcgga tgatgcaaat tttgtaaatg gggctgtgct atgtgtagat ggcggattaa
     5221 taatttagag ttacccggac aaagttaatg ttaacggcgg agccatctgg taccccgtag
     5281 gagcaagcgg tacaaggata ttagtcacac ttttgcatga attacagcga aggaaaggta
     5341 catatggtct tgcaactctc tgtattggag gaagccaggg tattgccatg gcggtaaaga
     5401 ggtaaataaa atgggaaaaa aagcaggatt ggaaggtaaa gtcgctctcg ttaccggggg
     5461 atcaaggggg ataggccagg cgatagcatt gcgtctggcg aaggatggcg cagatgttgc
     5521 aattaattat caatctacaa aagagcaagc tgagaagtta tcaaagttaa tagatcagat
     5581 gggtatgatg gatgaattcg ataggttatc cctgaagata gacctgatgg agacaaagga
     5641 gaacgcgaag gaagtatcag agttaataga ttctatgggc aaacattcta tcatatgcca
     5701 ggcgaacgtc aatgattttg aacaggtaaa caggatgcgg gacgaagtgg taaagcagtt
     5761 cggcagaata gatattctgg tcaacaatgc gggaatagtc agggataaat cttttgtaaa
     5821 aatgactcca cagatgtgga cggaagtatt atctgtgaac ctggatggtg cattctactg
     5881 taccaaagcg gtcatagagg gtatgctgga gaggaaatac ggtagaattg tgaatatatc
     5941 ctccgttatc gggagaatgg gtaaccgcgg acaggcaaac tatgcagcct caaaggcagg
     6001 actaatcgcc ctgtcgcaaa cactggcaaa agaatttgca ggcaagggta taacggtaaa
     6061 cgctgttgcg ccaggattta ttgaaacaga aatgctaaga tccgttccaa aagaaataat
     6121 ggataagatc cttgcccaga taccgctggg aagacttgga aagccggccg aggtggcagg
     6181 agcggtggca tatctggtct cagaagatgg ggaatatatg acaggtcagg tcattgacat
     6241 aaacggggga ctctacattt aatccagtcc cttcaggacg caggagggac tctcatcaaa
     6301 aggcttaaat gcatcaaagc cataatggaa aatcccccca aagtccggat agtgtagcgg
     6361 cctatcatgg aggcctgtcg agtctccgac ccgggttcaa atcccggccc ggacgttttt
     6421 ctttttttct tgttcacctt ttcaaaatgc ccactttttt cgggactcta caatatccat
     6481 tctccatcga aaagtatata agtcagtatt tataatgatt ataattatga taatgatgtg
     6541 gtggttaaga gaatgatatg tataattaga aaagcgtctc ctttatatct gaaaagatac
     6601 cagtaggaaa ttcatgatca agctctcgga actctattcg ggtaaggcaa agacagtttt
     6661 taagacagat gatcctgaaa agctgataat ggaattcagg gacagcctta cggcatttga
     6721 cggtaagaaa aaaagcgaag cgccaaaaaa gggatactac aatgcgcaga tagccgctaa
     6781 actcttaaca cttcttgagg agaaaggtat taagacccat tatgacagca tgatgtctga
     6841 taccgagatg gttgtggatg ctgtcgagat cattaagatc gaagtcattg taagaaatat
     6901 tgcggcaggt tcactggtca agaaatatcc cttcaaggcc ggacagaaaa ttgatcctcc
     6961 aatccttgtt ttcgattata agagcgatga gcatggcgat cctatgatca acgatgatat
     7021 cgcgcttgcg ctgggacttg caacaagacc ggagcttttg gaaatacgaa aacttgcact
     7081 tttgataaac tcaatactgg tcgattacct tggtgacaaa aatatcttac tcccggattt
     7141 caagcttgag ttcggacgaa gaaagggtaa aatcgtactc gcagatgaga tatcctgcga
     7201 tacatgcagg ttctgggata aggcaacggg agaatcgctt gataaagatg tgttcaggtt
     7261 cgataaaggc gatcttgtgg ctgcatatcg ggaagttgca cgaagagttg ttcctgaaat
     7321 attcaagtca acatgagagt tgcatggtgt attactggcg ccgggcattt tttatgcgaa
     7381 agctttgagg tttttaagga gcttcgagaa aaaaacaaag aattgaaagt cacatcgttt
     7441 gtatcgcgtg ccgcagaaga agtaataaaa atgtacggat tacaaaatga tcttctcaat
     7501 atttcaagcg gcggctacct tgaagagtgt ttttatgaac gggaacaggg ttcaagcttt
     7561 cccaaggcag gaagattcct tttgaataaa tacgatgccc tggtcgtcac ccccgcaact
     7621 tcaaatacaa tcgccaaact ggcctacgga atagcggaca ctcttgtcac caatgctgtg
     7681 gcgcaggcag taaaaggtaa tgttccggtt tatatagttc ccgttgacat tgaaggatat
     7741 atcgaatcaa aaatgccctt cttcatcgag agggaaattt gtatgaaatg cgaggtttgc
     7801 ccgcccatga aagattgccc gaataaggcc atcaccgacc agatcgatct tttaaaatgc
     7861 aatggatgcg gaatatgcgt aaaactatgc agttatggtg cgatcagggg agaaattaca
     7921 aagataaaag tccgtgatgt tgattcaaag aatgtgaaaa tattgagtga gcttgaaggg
     7981 ataacaattc tcaatcgacc cgcaagagcc cttgatctaa tagagcaatg caaaaattga
     8041 agtactaaag gataaatgaa gggttaataa agaaaataga ggcaaaaatc ttgttcccga
     8101 ctaaaaaggt tcaaacatta ataggttcaa ttgtacaggt tgagatgaaa ggagacaaac
     8161 atactcttga aggcaagctt gaaagcgcgg atgagtattt gaatctgcat cttaatgaca
     8221 catatgaaat tgcagatggt gtaaaatcgc gttccctggg ttcagtcatc ttgagaggca
     8281 ataatattat catgctaagc ccaataaagc agtagggaaa aagaatggat atagaggaaa
     8341 aaatcctgaa gttgataaaa tccaagaaaa atggaattct ccaaaatgaa ctctggaaga
     8401 cggcaaaaat agatagcagt aaatgttcga ggatcgtagc gaagctggaa aaggacagat
     8461 tgatcacaag agaacaaaat tcttcaaaag gtacaaagac cttccttatc agatcagtta
     8521 caaaaaaaga agaaaaagct aagaatttca agttattact atcgaaagag gtgttctcac
     8581 catgcacagg ttgctctctg gaatgtgttc ctgaaaattg catgcctctt tccgaatggg
     8641 ttcatggtct gcaaaatgaa tagttcatga aagagtcccc gggatctttc caagcgcttc
     8701 gtcgatatct tttgctaatt tatatggaat tcctacaacg acctcatcat ccccatattt
     8761 actgtgcttc cttgaaccct tgcatccaag agagacaccc aatttttctt g
//
LOCUS       DATGBN010000290         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_22324, whole genome shotgun sequence.
ACCESSION   DATGBN010000290 DATGBN010000000
VERSION     DATGBN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22324"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VKL21_07035"
     CDS             complement(<1..718)
                     /locus_tag="VKL21_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMB45558.1"
                     /translation="MKKKEKWQNRNVLVTGATGFVGPYLVKELLCREAWIKVLTMEKL
                     RNLSGLEDKIITVSGNITDPGSLQNIMKDVDIVFHLAAVSNVNYAISHPRETFETNAT
                     GTLNLLEEARKNEVEKFVYISSSHVYGIPQYLPMDEKHPVNPHEPYAASKAAAEMLVN
                     IYALNYGLKTTIIRPFNMFGPGQSGDFIIPSIISQALKKKVVELGNLTPTRDLLYITD
                     AIEGMLTIAEQGEGVYNIGSG"
     gene            complement(690..1670)
                     /gene="gmd"
                     /locus_tag="VKL21_07040"
     CDS             complement(690..1670)
                     /gene="gmd"
                     /locus_tag="VKL21_07040"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMB45559.1"
                     /translation="MTKSALITGITGQDGSYLSELLLGKGYKVYGLIRRLSTPNLARI
                     ENILDQITLIEGDLTDQSSINNALKESQPCEVYNLAAQSFVVTSWNQPVLTGDVTGLG
                     TVRILEGVRHVCPDAHFYQASSSEMFGKVNETPQNEISKFYPRSPYGFAKVYAHWACI
                     NYRESYGMHASSGILFNHESPRRGIEFVTRKITEGVARIYHGMANELRLGNLDARRDW
                     GFAGDYVKAMWLMMQEKEPDDFVIATGKAHSVGEFAKLAFDQVGLDWNKYVKVDPKFI
                     RPAEVNLLLGDASKARRVLGWEPEVTFEELVRMMVREDLKRYEEKRKMAK"
     gene            complement(1674..2258)
                     /locus_tag="VKL21_07045"
     CDS             complement(1674..2258)
                     /locus_tag="VKL21_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HMB45560.1"
                     /translation="MTSEINLKGSRTEKNILAAFAGESQARNRYTYFASAAKNEGFEQ
                     ISAIFLETADNEKEHAKVFFKHLQGGNVEITAMYPAGVIGKTADNLFAAAEGEKIEWG
                     TLYPGFAGIAQEEGFLKVAQSFTEISVVELFHEMRYRQLINNLKEGSVFKKKNSVKWH
                     CRNCGYIHEGMEAPKDCPACKHAQSYFEVLAQNW"
     gene            complement(2339..2494)
                     /locus_tag="VKL21_07050"
     CDS             complement(2339..2494)
                     /locus_tag="VKL21_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45561.1"
                     /translation="MIVDLLKGLSEKGQTIIVVTHDMDVARQTDRIITMRDGAIASDT
                     GGVTGYM"
     gene            complement(2491..2580)
                     /locus_tag="VKL21_07055"
     CDS             complement(2491..2580)
                     /locus_tag="VKL21_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45562.1"
                     /translation="MAIARALANAPAIILADEPTGAVDTKTRE"
BASE COUNT          627 a          657 c          512 g          810 t
ORIGIN      
        1 ttcctgagcc tatgttgtat accccctctc cctgttcagc aatggtgagc atcccttcaa
       61 tcgcatccgt gatatataac agatcccggg tcggtgtaag gtttccaagt tccaccacct
      121 tttttttaag cgcctggcta atgatggacg gaataataaa atccccggat tgtcctggcc
      181 cgaacatatt gaacggcctg attatcgtcg tcttcaatcc gtaattcagg gcgtaaatgt
      241 ttacaagcat ctcagcagcg gctttgcttg ccgcataggg ctcatgagga ttaacaggat
      301 gtttctcatc cattggaagg tattggggta tgccatagac atgagaagaa ctgatgtaca
      361 cgaatttttc aacctcattt tttctcgctt cctcaagcag gttcaatgtc cctgttgcat
      421 ttgtttcgaa tgtttccctg ggatgcgaga tcgcatagtt tacatttgat actgcggcaa
      481 gatggaaaac aatatcgaca tctttcatta tattttgcag tgagcccgga tcggtaatat
      541 ttccactaac cgtgattatt ttatcttcaa gacctgagag atttctcagt ttttccattg
      601 taagcacttt gatccatgct tctctacaaa gcaattcttt gacaagatag ggaccaacaa
      661 aacccgtagc gcctgttacc agtacattcc tattttgcca tttttctttt ttcttcatat
      721 ctttttagat cttcccttac catcattctt acgagttctt caaaggtaac ttcaggttcc
      781 cagcccagga cacgccttgc ttttgaagca tccccgagca gcaggttcac ttctgcaggg
      841 cggatgaatt tcgggtccac ctttacatat ttattccagt caagaccaac ttggtcaaag
      901 gcgagttttg cgaattcacc cactgaatgc gcttttcctg ttgctataac aaaatcatcg
      961 ggctctttct cctgcatcat tagccacata gctttaacat aatcgcctgc aaatccccag
     1021 tcgcgtctgg catccaggtt gccaagacgc agctcatttg ccataccatg atagatacgc
     1081 gccacacctt cagttatttt tcttgtcacg aactctatac cgcgccgggg cgattcatgg
     1141 ttaaacaaaa tcccgctgga cgcatgcatc ccgtaacttt ccctgtaatt gatgcatgcc
     1201 cagtgtgcat ataccttggc aaagccgtaa gggcttcgtg gatagaactt tgaaatctca
     1261 ttttgcggtg tttcattgac tttcccgaac atctcacttg acgaagcctg gtaaaaatgg
     1321 gcgtcagggc acacatgacg cactccttca agtatccgga cagttcccaa tcctgtcacg
     1381 tcccctgtga gtacgggctg gttccaggag gtaactacga aagattgggc agcaagattg
     1441 tacacttcac agggttggga ttctttcaaa gcattattga tggaggactg atctgtcagg
     1501 tcgccttcga tcagtgttat ctgatccagg atattttcta ttcttgcaag attgggagtg
     1561 ctaagacgcc tgatcagtcc atagacttta tatccttttc caagaagcaa ttctgacagg
     1621 taagagccgt cctggccggt gattcctgtg attagcgctg attttgtcat aatctaccag
     1681 ttttgtgcaa gtacctcaaa atacgattgc gcatgcttgc atgcggggca gtcttttggg
     1741 gcttccatac cttcatgtat atatccacaa ttcctgcaat gccatttcac cgaattcttt
     1801 ttcttaaaga cagaaccttc ttttaagttg tttatcagct gcctgtacct catttcatga
     1861 aagagttcaa caactgaaat ttccgtaaag gactgcgcta ctttcaagaa tccctcttct
     1921 tgcgcgattc ctgcaaatcc cggataaaga gttccccatt caattttttc tccttctgct
     1981 gccgcaaaca ggttatctgc agtcttcccg atgacgcctg ccggatacat tgccgtgatc
     2041 tccacattcc ctccctgaag atgcttgaag aagacctttg catgttcttt ttcattatct
     2101 gcggtctcaa gaaaaatcgc tgatatttgt tcaaatcctt catttttcgc agcgcttgca
     2161 aagtaagtat aacggttcct tgcctgggat tccccggcaa atgctgcaag tatattcttt
     2221 tcagtcctgc ttcctttaag atttatttct gatgtcatga ttctgtaatt ccttttccat
     2281 cttttcgggt tatgtgccga tactattata gaaaggacga atacataaac cattccagct
     2341 acatatatcc tgtcacacct cccgtatcac ttgcaatagc gccatcacgc atagtaatta
     2401 tccggtcagt ctgccgcgcc acatccatat catgcgtcac aacaatgatg gtctggcctt
     2461 tttcactgag ccctttcaag agatcaacga tcattccctg gtttttgtat cgacagctcc
     2521 cgttggttca tcagcaagga taatagcagg agcgtttgcc agtgcccttg caatagccac
     2581 gcgctgctgc tgtcctccgg ataact
//
LOCUS       DATGBN010000291         5581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_488711, whole genome shotgun sequence.
ACCESSION   DATGBN010000291 DATGBN010000000
VERSION     DATGBN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488711"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VKL21_07060"
     CDS             <1..301
                     /locus_tag="VKL21_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45563.1"
                     /translation="AQGGGFVTMLANKATPGSVEDELLGLIDVIIRVDEEKIHARIGS
                     KELSIPFCFTGSELILGNVDSDKELQDLFSLTPDEKKKLELEVEEKTHLYGEPTG"
     gene            complement(280..1020)
                     /locus_tag="VKL21_07065"
     CDS             complement(280..1020)
                     /locus_tag="VKL21_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HMB45564.1"
                     /translation="MWISFHSYKGGTGKTNISGNLACYLVSKGYKVGLIDTDFSGPGI
                     HALFSLKTKKTLVDYLQDKASLEDIVYRRGSGELYIIPSDATEEDIVSFSKDPAQAKE
                     KLQALLRDFQSKYNPDHIIFDCSPGLNRSSLLIMGTTEISIVVCTLDKQDIRGTYVIS
                     GVAQRLKSRPRILFNKLPKNKYEASKGTLEDLSRELGLEPIGVILYDEAMACAWSRKI
                     MVHAYPSSEYSRQITELWERINQPVGSP"
     gene            complement(1055..1459)
                     /locus_tag="VKL21_07070"
     CDS             complement(1055..1459)
                     /locus_tag="VKL21_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45565.1"
                     /translation="MNSNMEIRTNDDLKAFERRIQSLERRVGEIESRLHPVVEDQIAL
                     YLTIPDSLRKSLFAVSELLKATADDVSIRTGRHRSIENKYLNELVRSGWLKRERIGKK
                     VYYSSKKKKEEESPEVIGSIEELDKKLEKILG"
     gene            1932..2636
                     /gene="mqnB"
                     /locus_tag="VKL21_07075"
     CDS             1932..2636
                     /gene="mqnB"
                     /locus_tag="VKL21_07075"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HMB45566.1"
                     /translation="MPKIALIAPTSLESEDLRREFKTSLDEKQGVISKGYLHGKSIVF
                     SHCGVGKVNAAHRTTLVLERNDIDYLILFGVGGAYSDANVGDVAVAESENYAEEGVII
                     GEGWKPMDFTGFALLKNEIEYFNTFPMDRKLMQLAINASRDVGLYAICGNFITVSQCS
                     GTRVSGEIMQKRFNGICENMEGAAVAHICAIYGIPMIEIRGISNMIEDRNLKKWNVPL
                     AASNCNKAVNEIIKRL"
     gene            2994..3848
                     /locus_tag="VKL21_07080"
     CDS             2994..3848
                     /locus_tag="VKL21_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB45567.1"
                     /translation="MNKINKIIVGILTIFLFIGTVSATNNEVTFGWITDFHYTKSSDF
                     NTFNNFISKLESSNANYVIGTGDLIDSNSIESDLTEIVNKCKSSEVLKCYYTLGNQEI
                     ETIGIEKWLSITGMDSENYYIDTDWGRLIFLNTQFSLSSEGLEWLKSTVDTKNRIVVF
                     NHFPLNGGYISNDDEIRRILESKGNVVAVFSGHIHTNSNIKTNNIMYLTQSTMENGDY
                     SIVKLRDSDIQIIGDGAASITFSIPNTVDGNDDKNIGEEKDDKEHKEHKEHKEHKEDR
                     HDGGHDDD"
     gene            4066..4893
                     /locus_tag="VKL21_07085"
     CDS             4066..4893
                     /locus_tag="VKL21_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HMB45568.1"
                     /translation="MKKFSLGYSPCPNDTFIFYALTHYRLACDIEFRETLKDVEYLNR
                     MALHKRLDITKASFHGFGFLRDDYCLLHSGSALGRGCGPLIVARDGMDQKDLVMKKIA
                     IPGRMTTAYLMLQLFAPDAKNIVEMPFDRIMNAVSMGTVDAGLIIHESRFTYPQYGLV
                     KILDLGEWWEKETGLLIPLGGILARRDMGAAVIQKIDDLIKRSIEYAFQHPEEPREYI
                     KKNAQELDDDVIDQHIKLYVNDHTLDIGDGILAVEKLLKTAEELNLIPHSGNTIFFN"
     gene            complement(4890..5207)
                     /locus_tag="VKL21_07090"
     CDS             complement(4890..5207)
                     /locus_tag="VKL21_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45569.1"
                     /translation="MASELKINSDDEYSLSAVNTKNEVINVLVRKCHHCNEIVPIVGL
                     CSTCNTGYEHRKTVENPETGFIHFIYECSGCPPAKRKAQKIIKINDGNNRQSSDDLIK
                     NFL"
BASE COUNT         1784 a         1033 c         1106 g         1658 t
ORIGIN      
        1 tgcgcaagga ggtggttttg taaccatgct ggcaaataaa gcaacaccgg gcagcgttga
       61 agatgaacta ttaggactca ttgacgtaat aatcagggta gatgaagaaa aaatccatgc
      121 acggatagga agcaaagaat taagcatccc cttttgtttc acaggaagcg aattgatcct
      181 tggaaatgtt gattcagata aagaattaca ggatctattc agtctgactc ctgatgaaaa
      241 gaaaaagctg gagcttgaag tagaagagaa aacacatctt tatggagaac caaccggttg
      301 attgatacgt tcccatagtt ctgtgatctg tctactgtat tccgagctgg gataagcatg
      361 caccattatc tttcttgacc atgcgcaagc cattgcttca tcatacagga ttactccaat
      421 aggctcaagc cctaattccc ggctcaggtc ctcaagtgtt ccttttgaag cttcatactt
      481 attttttgga agtttgttga acaaaatcct gggtctggat ttcaaacgtt gggccacccc
      541 tgaaataaca tatgtcccac ggatatcctg cttatccaga gtacatacta cgatactgat
      601 ttctgtcgta cccatgatca gcaggcttga cctgttaagt ccgggactgc aatcaaagat
      661 aatatggtcg ggattatatt ttgattggaa atcccgaagc aacgcctgaa gcttttcctt
      721 tgcctgcgcg gggtcttttg agaaacttac tatatcttct tctgtggcat ccgagggtat
      781 tatataaagc tccccactac ctcgcctgta cacgatatct tcaaggctcg ctttatcctg
      841 aaggtaatcc actaacgtct tttttgtctt caggctaaaa agtgcatgga tgccaggacc
      901 agaaaaatca gtgtctatca gtccaacctt atatccctta ctaacaagat aacaggcaag
      961 atttcccgaa atattcgtct tgccggtccc ccctttatat gaatgaaaac ttatccacat
     1021 ttcttgcacc ttaaattgat tattctatgc ataattaacc cagtatcttt tcaagttttt
     1081 tatccaattc ttctattgat ccaattactt caggagactc ttcctctttc ttttttttcg
     1141 atgaataata cacttttttt ccgatacgtt cacgtttcag ccagcctgat cttacaagtt
     1201 cgttgaggta tttgttctct attgacctgt ggcggcctgt tcgaatgctc acatcatcag
     1261 ccgtagcttt caataattct gagaccgcaa aaagggattt tcttaaactg tcaggaattg
     1321 tgaggtataa agcgatctgg tcttccacga cggggtggag gcgcgattca atctcaccta
     1381 ccctgcgttc gagtgactga atccttcttt caaaggcttt taaatcatca tttgtgcgga
     1441 tttccatatt tgaattcaca ttatctccaa tgcacatgtg caagttgatc gcagaatttt
     1501 gaaagcgctg agcaatcttt gcacactatg ttaatagggt acaagcacta tataaatcta
     1561 tagtcatttc gtgaccgtta cattacatgt tggtataatt ttttgcatca ttattttttt
     1621 ttgacaggat cgacaggatt tacaggattt cctacacaaa ccgtatcctg tcgatcctgt
     1681 aaatcctgtc tgaagtttat tgatttcatg atctgcggtt ccttttcttc agaaccgcag
     1741 atgaacgcaa cataaatgca tatgttgcta ttttgcacaa tatttatata ctatcagttg
     1801 tatattggat aactgtgagg aagttgcaca ataaacgcct cttcatccgc cctccatatt
     1861 gcaactttcc tcttatattt tttatctagc ttcggataag ctatatccca tgaaataaat
     1921 atgacttcca tatgccaaaa attgcgctca tcgcgcctac atccctcgaa tcggaagatt
     1981 tgaggcggga gttcaaaact tcactggatg aaaaacaggg agttatttca aaaggttatt
     2041 tgcacgggaa atccatcgtt ttttcccatt gcggggtagg aaaagtgaat gctgcgcaca
     2101 gaacaacact cgtccttgaa agaaacgata tagattatct gatccttttc ggggtcggtg
     2161 gcgcttattc ggatgcaaat gtcggagatg tggcggttgc tgaaagtgag aattatgctg
     2221 aggaaggtgt aattattggc gaaggctgga aacccatgga tttcacagga tttgcattat
     2281 tgaagaatga gatcgaatat ttcaatacct tcccgatgga caggaaattg atgcagcttg
     2341 cgataaatgc ttcaagagat gtcggtttat acgcgatttg cggcaatttt ataaccgtat
     2401 cccagtgttc cggaacaaga gtcagcggtg aaatcatgca aaagcgcttt aatggcatat
     2461 gtgaaaacat ggaaggagcc gctgttgctc atatttgtgc catttacggg atccccatga
     2521 tcgagataag ggggataagc aatatgatcg aggacaggaa tctgaaaaaa tggaacgtac
     2581 cccttgcggc ttcgaattgt aataaggcag tcaatgaaat tatcaagagg ctttagttaa
     2641 tcacccataa gtgtgggaat gtcaagtgag ggatatggga aacaggttga cagggtgaaa
     2701 gcaccaataa tttgccatac aggttctagt gatatttaat ctaacattat gaatttattt
     2761 acatgcctat gaatatgcat atatataagc ctgtactata taattactga ttatggtgtt
     2821 atcgggccaa cgtcggagca tccatatcaa aatttttttg aaaggaaaac aatggtggtg
     2881 gacaaaatag aagaaagaag aatcaacgat agggacttag caagcctgaa tgatgtcaaa
     2941 tagttgacaa tataataaaa caaggcagag gctttatata aggagtgaaa gaaatgaaca
     3001 aaataaataa aatcattgta ggaatactaa caatatttct atttatagga acagtatcag
     3061 ctacaaacaa cgaagtaaca tttggctgga taacagattt tcattacaca aaatcaagcg
     3121 attttaatac atttaacaac tttatatcaa aattggaaag ttcaaatgca aattatgtca
     3181 taggaactgg tgatctaata gatagtaatt ctatagaatc agaccttaca gagattgtta
     3241 ataaatgtaa aagcagtgaa gttttaaaat gttactatac tctcgggaat caggaaatag
     3301 aaaccattgg aatagaaaaa tggttatcta taactggaat ggattcagaa aattattata
     3361 tagacacaga ttggggaaga ttaattttct taaatacaca attttccctt tcatcagaag
     3421 gacttgaatg gttgaaatct actgtagata caaagaatag gattgttgta tttaatcact
     3481 ttcctcttaa tggtggatat ataagtaacg atgatgaaat tagaagaatt ttagaatcaa
     3541 aaggaaatgt agtagcagta ttttcaggac atattcatac aaattctaat attaaaacga
     3601 ataatataat gtatttaaca caatctacaa tggaaaatgg cgattattct attgtaaaac
     3661 ttagagattc agatatacaa attataggag atggtgcagc ttcgataaca ttttctattc
     3721 caaacaccgt tgatgggaac gacgacaaga acattggaga agaaaaagac gataaagaac
     3781 ataaagaaca taaagaacat aaagaacata aagaagatag acacgacggc ggacacgatg
     3841 acgactaaag aggcaacaga taagaaattg ttctaggtaa tctccaatcc tttcaataaa
     3901 aatgcaaatt gaggatgatc cccctaatcg gggtttttcc ctcaatgaat aaaaccatat
     3961 acactttgat tgattcttcg catctattaa gtttccaaga gcaaatcctt tgttgttcca
     4021 agcattaaca tcatgtagat cgatcttcaa tgattacgga gtattatgaa aaaattctca
     4081 ttaggttatt caccatgccc caatgacact tttatcttct atgccctgac ccattacagg
     4141 ctggcatgcg atattgaatt cagggagact ttaaaagatg tggaatacct gaaccgcatg
     4201 gccttgcata aaagattaga tataacaaaa gcctcatttc atggatttgg ttttttgagg
     4261 gatgattatt gtctcctgca ttcgggcagc gctcttggac gcggttgcgg gcccctcata
     4321 gtcgccaggg atggaatgga tcaaaaagac cttgtcatga aaaagatagc tattcccggc
     4381 agaatgacaa ccgcttacct gatgctccag ctgttcgcac ctgatgcaaa aaatattgtt
     4441 gagatgcctt ttgacaggat catgaatgca gttagcatgg gtactgtgga tgcgggtttg
     4501 atcatccatg aaagccgttt tacttatcct caatatggtc ttgtaaagat actagacctg
     4561 ggtgaatggt gggaaaaaga aacaggttta ctgattccgc tgggagggat ccttgcacga
     4621 agagatatgg gtgcggccgt gattcaaaaa attgatgatc taattaaacg aagcatagaa
     4681 tatgcattcc agcatcctga agaaccccgg gaatatatca aaaagaatgc acaggagctc
     4741 gatgacgatg ttatagacca gcatattaaa ctatacgtaa atgatcatac cctggatatc
     4801 ggggatggaa tcctcgcagt ggaaaaattg ctgaaaaccg ccgaagaatt aaacctgatc
     4861 ccgcattctg gaaacacgat tttctttaat taaaggaaat tctttatcag atcgtcactt
     4921 gactgcctgt tgttgccatc gtttatcttg ataattttct gggctttcct ttttgccggg
     4981 gggcaacctg aacattcata tataaaatgg ataaaacctg tctcaggatt ctctaccgtc
     5041 tttctatgct cataacctgt gttgcatgtg gaacataatc ccactatcgg gactatttca
     5101 ttgcagtgat ggcattttcg aaccagaaca ttgatcactt cattctttgt gtttacagca
     5161 gataacgaat attcatcgtc gctatttatt tttaattctg aagccatatt ccaaatcaac
     5221 ttaataacaa caggatatat taaaacttta cgagtttcct ggggtaatcc caaaatcctg
     5281 caaaacaaaa catttattca tacttgtatt ataataacta ttgaggtgaa cagtgtgact
     5341 gaatatttta aatctaaaat agattatcta cgcgctttgg aaaagagttg cttgaattgc
     5401 ggcgacaaac atcaacagga ttgcccggtg gggatggcaa aagcaaaaat caactgtacc
     5461 aacgatttat taaccgaatt ttataaacgc aaataaacca aaacctatta tattcctgaa
     5521 ggtatatggg catcttgtga ctaaaatagc agtgcttgta tcaggaagag gttcaaacct
     5581 g
//
LOCUS       DATGBN010000292         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_411578, whole genome shotgun sequence.
ACCESSION   DATGBN010000292 DATGBN010000000
VERSION     DATGBN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411578"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VKL21_07095"
     CDS             <1..277
                     /locus_tag="VKL21_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45570.1"
                     /translation="NPLDSINTADLEGLLSGDKKKVNAEQDLLFTKAVKLTLDDVEFV
                     KDMAIAVKSEGNFSEGIRACIKAARKYYGDNVKELANQRKSLSAIEI"
     gene            723..1373
                     /gene="tmk"
                     /locus_tag="VKL21_07100"
     CDS             723..1373
                     /gene="tmk"
                     /locus_tag="VKL21_07100"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HMB45571.1"
                     /translation="MKIPVIAMKNGILIAAEGIDGAGKRTICSFMKELLESYEYKVTT
                     FAYPDYYNIWGKIIEDYLHNKIELNINEQFFAYFIDILKDKDKIAELLRRGNIVITDR
                     YFSSPVAFQCAKGFDYQKALSIIRMMDIVEPDLTLFIQIPPKLACHRKYQQKNYLDRH
                     EKDLKLLEDVDIMYEKMLSQKILSKKWVKINGDQDPKKIEENIQNILNMFLGKNLG"
     gene            complement(1600..1890)
                     /locus_tag="VKL21_07105"
     CDS             complement(1600..1890)
                     /locus_tag="VKL21_07105"
                     /inference="COORDINATES: protein motif:HMM:NF036995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HMB45572.1"
                     /translation="MKAVNKELISLYWDIGQMIVERQKGKTWGKSVVEQLAKDLQIEF
                     PGMQGFSSRNIWRMRSFHLIYEVNPKLPPLVAENRLDTQLHHFRKVQRQSGA"
BASE COUNT          667 a          402 c          417 g          665 t
ORIGIN      
        1 gaatcctctt gattcgatca acacggcaga ccttgaagga ttgctcagcg gtgataagaa
       61 aaaggtcaat gctgaacaag atctgttgtt tacaaaagcg gtcaagctga ctctggacga
      121 tgtcgagttt gtaaaagaca tggctattgc tgtaaagagt gaagggaatt tctcagaagg
      181 aattcgtgcc tgtatcaaag ctgcccggaa atattacggg gataatgtta aagaacttgc
      241 caatcagcgc aaatcgcttt cagcaataga aatataatgg actgcatttc ttccagtaga
      301 cagtaagtta aattgtaaat taatttactg ttttactggt ttacttttta atcatttcgt
      361 ctctacatgc tgttttccat gtcggaagaa gactctcagg attcaaggat attgaaagaa
      421 gcgaagaggt tgatgtcttg caatcaatct tgaggtattc aaataaagct gtttagatat
      481 tccatatgtt ccacatgaaa tgatatatta tgtgtgtgta atagttccac tggaaatcag
      541 cgcaaaagtc accggggcct gaatatatcg atatcattat tacttgttgt ttttcatttc
      601 ttcccgcgcc cccaacctct caccttgggc acctccggct tatgaataaa aagtaacttt
      661 taacagcggg ctgtttgtga tttctgagga gttaaatgta aaattaggag atatttatta
      721 ccatgaaaat ccctgtgatt gcaatgaaga acggaatttt gatagctgcc gaaggtatag
      781 atggagcagg aaaacgcact atatgttctt ttatgaaaga gttattggaa tcatatgaat
      841 ataaggtaac tacattcgca tatcctgatt actataacat ttgggggaaa atcattgaag
      901 attatcttca taataaaatc gaacttaata taaatgaaca atttttcgca tactttatag
      961 atatattaaa agacaaagat aaaattgctg agttgttgag aagaggtaat attgtgataa
     1021 ctgatagata tttttcttct ccagttgcgt ttcaatgtgc caagggtttc gattatcaaa
     1081 aggcgttgtc aataattaga atgatggata tcgtcgagcc cgacttgact ctgtttatac
     1141 agataccacc aaagttggca tgtcatagga aatatcaaca aaaaaattat ttggatcggc
     1201 atgaaaaaga cctcaaattg ttagaagatg tggatattat gtatgagaaa atgctaagtc
     1261 agaaaatatt atccaaaaaa tgggttaaaa taaatgggga tcaggatcca aaaaaaatcg
     1321 aagaaaacat acaaaacatc ctaaatatgt ttctaggaaa gaatcttggc taatctcctt
     1381 ctattcattg ggagtgcaac ggactttgtt ggaacatttt aaaccaacct gcttctggat
     1441 tattacttac taccaccccc tccacaaacg tcggatgaaa caacacctgc tcagctcaaa
     1501 tcagacttat cattcaaaga tatctttatt ttcacaatct tgtggcgaca ttactgtaag
     1561 cctaacaaaa gattaatcag tgtcatgcgg atatagaatt cacgctccag attgtctttg
     1621 cacttttcta aaatgatgta gttgtgtgtc cagtcgattt tctgccacca atggtggcag
     1681 ttttggattg acctcataaa ttagatggaa gctgcgcatt ctccagatat ttctggatga
     1741 aaaaccttgc atccctggga attcgatctg aagatccttc gccaattgct cgacaactga
     1801 ttttccccag gtttttcctt tctgtctttc aacgatcatc tgaccgatat cccagtagag
     1861 cgatatcagt tccttgttaa cagccttcag cgcctcatac tgagcagagc ggacacgcta
     1921 cttgatatcg ctaagaagaa taacatagtc tttcggaatc atatctgtaa tgctctaacc
     1981 ccatccctgc gtcatttctt tcatttttat acgttcttcc ggggttacgt cgttattgta
     2041 gtaatgcttc attgatgtgt tcatgtcatg tccctgagaa gccaggattc gactaagagg
     2101 ccagcccgat tctattagcc atgactccca ggcctttcgc gtagatcctg c
//
LOCUS       DATGBN010000293         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_101069, whole genome shotgun sequence.
ACCESSION   DATGBN010000293 DATGBN010000000
VERSION     DATGBN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101069"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..281)
                     /locus_tag="VKL21_07110"
     CDS             complement(99..281)
                     /locus_tag="VKL21_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB45573.1"
                     /translation="MADELMHSPELTIDSEKWLSDAITMFKRCKASHIAVVEQGKKVG
                     IIRVDDILHTYRYNNR"
     gene            complement(271..402)
                     /locus_tag="VKL21_07115"
     CDS             complement(271..402)
                     /locus_tag="VKL21_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB45574.1"
                     /translation="MSKISHILLPNPVVVPTGTKLNEVAQLMKKKRIASALVSKNGR"
     gene            888..1421
                     /locus_tag="VKL21_07120"
     CDS             888..1421
                     /locus_tag="VKL21_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HMB45575.1"
                     /translation="MVIIKGHEIETVIFKNAHNRRAMQLKNNIVMLLRSIGVKENDID
                     VPVENVAIKKARASATWYYCGHRMYYSHNMQSKFVDNLHVLFKVIEMESNLVLFEKKT
                     FDDFISEFREDSDVDEKRKEARDFFGVDPDLNDLEIISKKYKAMAKELHPDMPTGDTE
                     KFKQLNIAHKILKRELT"
     gene            1433..1795
                     /locus_tag="VKL21_07125"
     CDS             1433..1795
                     /locus_tag="VKL21_07125"
                     /inference="COORDINATES: protein motif:HMM:NF018621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HMB45576.1"
                     /translation="MKAKLRGHHLICLNFFRGEGYSDDFIENIYEVLKKEKVEIIAGP
                     DEVCSKCPYLKRGKCANNEYSDEMILAQDHEALRLLDFMPGETIDWKMIAQRLPDILD
                     EWKAQFCAGCGYRKVCLK"
     gene            1890..2477
                     /locus_tag="VKL21_07130"
     CDS             1890..2477
                     /locus_tag="VKL21_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMB45577.1"
                     /translation="MKELNFPIVKDDDNSAAGSTAAAKALIAEGVQIVIGSSGSSVSA
                     AMATELGSQKIPQISYAASSPSLSDRSLYPYFMRVVGSDADQGKAIADLVQVFGWKRG
                     AAIQTSDSYGAGMISVFIDIFKKRGGSIVTDQQFDPGATDVSAQVQTKVDANPEFVLG
                     RFIDVDAATAVKKAWELGAIKRNWITTDGWSTGIQ"
     gene            complement(2840..>3232)
                     /locus_tag="VKL21_07135"
     CDS             complement(2840..>3232)
                     /locus_tag="VKL21_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HMB45578.1"
                     /translation="LGGIVFGIISGALSGDKSMAIEAIGGAIGGILISLVLMIIFGFI
                     IPMALAMYIKEDNISAAFRFGELLSRIRSVFGEYLTVYIVLIVLSFVLGLFGAVPILG
                     FFILIFGGFYIEVVGANMFGKVYAKSTR"
BASE COUNT         1022 a          615 c          647 g          948 t
ORIGIN      
        1 atgctatgtc ccctgttctt tgcccaaact tcgatatttc ccaaaccttc aaaaggttca
       61 tgctcaatgg aatgaattct catgaatatc ccgatctttt acctattatt atacctgtat
      121 gtatgcaata tatcatctac cctgataatg cccaccttct ttccctgttc caccactgct
      181 atatgtgaag ccttgcagcg cttgaacatt gtaatagcgt ctgaaagcca tttctccgaa
      241 tctatggtaa gttcaggaga atgcatcaat tcatctgcca tttttgctga caagcgcact
      301 tgcgattctt ttcttcttca tgagttgtgc aacctcattg agttttgtcc cggtgggaac
      361 taccaccggg tttgggagga ggatgtgact tattttggac attattattt accccttttt
      421 atttcgatat gaattaacca ctgagatttc tctgcctatc acccaatcct gactttcggc
      481 tcgcaaacaa tactcttctt ggggcgtttg tcatctggtc aagcagatat atcgagtcat
      541 tgtagcccat gatggaatac ctgtatacac caacgcggtc gtatacaggg aatccaaagc
      601 gcactaaggg gacaacctca tcatctgcta catccttacc cttggagttg ccaagtatca
      661 gttcagcgcc atgggttttt aaaattaata attaattcaa gcaacaaatt tatcccattc
      721 attcggtttc ttgaatagca tggataagtt tatgccccaa tggtaattat aagaagagct
      781 aactcggtgg ttttagttat caaatttaga gttactaaat atcttatgag ataatatggt
      841 tgacaaaaca ccagtaaagt cttcaaaaac gaaaatgagt gaattaaatg gtaataatca
      901 aaggacacga aatagagaca gtaattttta agaatgctca taatcgaagg gctatgcaat
      961 tgaaaaataa tattgttatg cttttaagaa gtattggcgt caaagaaaat gacattgatg
     1021 tccctgttga aaatgtagcg ataaaaaaag ccagagcttc tgcgacctgg tactattgcg
     1081 gccatcgaat gtattacagt cataacatgc aaagtaaatt cgttgataat ctccatgttc
     1141 tattcaaggt aattgagatg gagtccaacc tggttctttt tgaaaaaaaa acttttgatg
     1201 actttatttc agagttcagg gaggattcag atgttgatga aaaacgcaaa gaggcacgag
     1261 acttttttgg cgttgatcct gatttgaacg atttggagat aatttccaaa aaatacaaag
     1321 ctatggcaaa agaattgcat ccggatatgc ctaccgggga cactgagaaa tttaagcagc
     1381 taaatattgc tcataaaatc ttaaaaagag aattaaccta gacaatattt tcatgaaagc
     1441 aaaacttcgt ggtcatcatc ttatatgcct caatttcttc aggggtgaag gctattcaga
     1501 tgatttcatt gagaatatct atgaagttct caagaaagaa aaagtggaaa tcatagctgg
     1561 accggacgaa gtatgttcaa aatgccccta tcttaaacgt ggaaaatgtg cgaacaatga
     1621 atattcagat gaaatgatac tggcacagga ccatgaagcc ctgagattac tggacttcat
     1681 gccgggagag acaattgatt ggaagatgat cgcgcaaaga ttacctgata tcctggatga
     1741 gtggaaagca caattttgtg cagggtgcgg atatagaaaa gtatgtttaa agtgatcgat
     1801 ttcgagaagg cgttaagttt aaatatagaa gcttaacttc ttgtccactg gaaggggcgc
     1861 agtccagact ggaactaata gaatacttcc tgaaggagtt aaactttcct attgtaaaag
     1921 atgatgataa ctcagcggca gggagtacgg ccgctgcaaa ggcactaatt gccgaaggcg
     1981 tacagatagt tattggttct tctggaagta gtgtaagcgc agcaatggct actgaacttg
     2041 gttcgcagaa gattccccaa atttcttatg cagcatcttc tccatccctg agtgatagat
     2101 cgctctaccc ctactttatg agagtagttg gatcagatgc tgatcaaggg aaagcaattg
     2161 ctgaccttgt tcaagttttt ggctggaaaa gaggagcagc aattcaaaca agcgattctt
     2221 atggagcggg tatgatctcg gtctttatcg atatatttaa aaaaagaggg ggttctatag
     2281 ttactgatca acagtttgat ccaggtgcaa ctgatgtatc tgcgcaggta cagactaaag
     2341 tagatgcgaa tcctgaattt gttctggggc gtttcattga cgttgatgca gctacagcag
     2401 tcaagaaagc atgggaattg ggcgcaatta aaagaaattg gattactaca gatggttggt
     2461 cgacagggat tcaataaaaa taaatattga tatttaacag tttcttttct gattaaagca
     2521 aacggattca ctatagacaa atatggttat tttcaggata tgtcactgga aaactgtatg
     2581 gagaataaga ttggatgggc cttaagaaat gaacatgata atgataacta ttatactaag
     2641 cattaaatct ttaagaatct atatactcca gtgaatacat attgagtatt gttaaataaa
     2701 tgataggttg tgtatcagca aaggtggcga agctcctctc gccaccctgc tgcattattc
     2761 cgggttactt cgcattcctg tcttcttccc tcaggatcat ccgctggcag gtcaaaatgg
     2821 actattgttg gcatttccat caccttgtgg attttgcata tactttgcca aacatattag
     2881 caccaaccac ttcaatatag aatcctccga aaatcagaat aaagaagccc agaattggta
     2941 cagcaccgaa taatcccagg acaaacgata ggactatcag gacaatatat acggtcaaat
     3001 attccccgaa tacggatctt attctggaca ataattcccc gaacctgaac gctgcgctta
     3061 tattatcctc ttttatatac atagcaagag ccattggaat gataaatccg aaaattatca
     3121 taaggacaag acttatcaaa atccctccga tagctcctcc aatcgcttca attgccatac
     3181 tcttatcacc tgataatgca cctgatatta tcccgaatac aattccgccc aa
//
LOCUS       DATGBN010000294         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_178599, whole genome shotgun sequence.
ACCESSION   DATGBN010000294 DATGBN010000000
VERSION     DATGBN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178599"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="htpX"
                     /locus_tag="VKL21_07140"
     CDS             <1..828
                     /gene="htpX"
                     /locus_tag="VKL21_07140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HMB45579.1"
                     /translation="LVVLYFVFLAVILTFGGGYTNILLIFMTLMVFAQFFFSDRLVLW
                     STGGRVVTEQEEPRLHETVARLCAIADLPKPRIAIVDSNVPNAFATGRSKKSAVVAVT
                     TSLKQRLSQPELEAVLAHELSHVKNRDMLVITIASFLSTVAALLARNMLFFGMGGRDD
                     RKNGGAGIIIFVVSIVVWIISFLLIRALSRYREFAADRGSAMITGQPSHLASALMTIS
                     GVMERVPSRDLREVESMNAFFIIPAISGDSIMNLLSTHPSVEARIRALRDMEQKMEF"
     gene            828..1385
                     /locus_tag="VKL21_07145"
     CDS             828..1385
                     /locus_tag="VKL21_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45580.1"
                     /translation="MGFLDTLLGKSKLPKPKSDKLFAMSTAYVTLSSNLGLRSEGAGI
                     CFKPLEASSFQNAELEINQLLAQSCKETNTLHNMKKDSYGYLWVILSDPDFEDLVSTI
                     YMVSETLTDHDFGEQLLCAVFKFKGEKEVFWIYNFKQAKYYPFVPIGKQRDSSYEFRL
                     RSIMEKELPIEKETQRWYPLWDIPV"
     gene            1382..1780
                     /locus_tag="VKL21_07150"
     CDS             1382..1780
                     /locus_tag="VKL21_07150"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HMB45581.1"
                     /translation="MTCILFFDGGCRGNPGPMAIGTVLLENGKKVKEISKNIGIGTNN
                     IAEWKALIEGLKLATSHDCKELEVRGDSQLIIRQISGKYKVKSDNLIPLFNEARKLCG
                     NFKKIDFKWIKRDENSYTDNMVNNALDMEK"
     gene            complement(1825..>2569)
                     /locus_tag="VKL21_07155"
     CDS             complement(1825..>2569)
                     /locus_tag="VKL21_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMB45582.1"
                     /translation="VASDSQKAAERADEADKTAKEINIMSHELFRLMGEIRKAVGNSS
                     DLILELDIKSQKIGETTRSIAKIAYQTNMLALNASIEAARAGEHGKGFAIVADEVRKL
                     AEESKTAADRANELIKDIQEKTKNAVESMGRGTKVVVEGEGLIEKTVSSINNISEASG
                     NTAIMFHEIAAASEEQSVSNEEITASVRDVETIIERSAKALQETSVSIKEQTALMDDI
                     LKATQELVMLANELENDVVRFNLGGLKSK"
BASE COUNT          688 a          552 c          571 g          758 t
ORIGIN      
        1 cttgttgtat tatattttgt atttctcgcc gtgatattga cttttggcgg gggctataca
       61 aatatcctgt tgatatttat gacgctcatg gtgttcgccc agtttttctt ttcagatagg
      121 ctggtactat ggagcaccgg aggacgggtt gtcacagaac aggaggagcc gcgccttcat
      181 gaaacagtgg caaggttatg tgctattgcg gacctcccaa agccaaggat cgctatcgtc
      241 gacagcaatg tacccaatgc atttgcgacg ggccggagca agaaaagtgc ggtggttgct
      301 gttaccacat ctttgaaaca aagattgagc cagcctgaac ttgaagctgt tcttgcccat
      361 gaattaagcc acgtaaagaa tcgcgatatg cttgttatca caattgcaag tttcctgtcc
      421 acagttgcag ctttacttgc caggaacatg ctatttttcg gaatgggtgg aagagacgac
      481 cgaaaaaacg gcggggccgg gataattatt ttcgtagtct cgatagtagt atggataata
      541 agttttttgc tgatccgcgc cctttcaaga tacagggagt ttgccgctga caggggttcg
      601 gcaatgataa caggccagcc atcgcacctt gcatcagccc ttatgactat cagcggcgtc
      661 atggaacgtg tgccttcccg tgatttgagg gaagtggaat caatgaatgc gttctttatc
      721 atacctgcga tctcaggaga ttccattatg aatctcttat ccacacatcc ttctgttgaa
      781 gcgcgcatac gcgcacttcg ggatatggaa cagaagatgg agttttaatg ggtttccttg
      841 atacacttct tggtaaaagt aaactcccaa agccaaaatc cgataaactc tttgcgatgt
      901 caacagccta cgtcaccctc agttcaaacc ttggcctgag gtctgagggc gccggtattt
      961 gtttcaagcc gcttgaagcc tcaagtttcc agaatgcaga gcttgagatc aatcaattgc
     1021 tcgcgcaaag ctgcaaagaa acgaatactc ttcataatat gaaaaaagat tcctatgggt
     1081 atctgtgggt catcctttcc gacccggatt ttgaagacct tgtaagcacg atctatatgg
     1141 taagcgagac cctgacagac catgatttcg gagagcagct gctatgtgcg gtctttaaat
     1201 tcaaaggtga aaaggaggta ttctggatat ataatttcaa acaggcaaaa tattatccat
     1261 ttgtgccgat cgggaagcag cgggattcat cctatgaatt caggcttcgc tccataatgg
     1321 aaaaagagct gcctatcgaa aaagaaaccc agcggtggta tccgctgtgg gatattcccg
     1381 tatgacatgc atattgttct ttgatggggg atgcagggga aaccccggac caatggcaat
     1441 tggcaccgta ttattggaaa atgggaaaaa agtgaaagaa atttcaaaaa atatcggtat
     1501 tgggacaaac aatatagctg agtggaaggc gcttatcgaa ggattaaagc tggctacttc
     1561 tcatgattgc aaagagcttg aagtgcgcgg tgacagccag cttatcatcc ggcagatctc
     1621 agggaagtac aaagtgaaaa gcgataatct catcccgtta ttcaatgaag cccggaaatt
     1681 atgcgggaat tttaagaaaa tagatttcaa atggatcaaa agagatgaaa atagttacac
     1741 tgataacatg gtgaataatg cgctggatat ggaaaaatga ccaccattta gcacgttgcc
     1801 attaagattg ctattattct gctatcattt gcttttaagg ccgccaagat tgaatcttac
     1861 gacatcattc tcaagttcat ttgcaagcat tacaagttcc tgtgtagctt tcaaaatatc
     1921 atccatcaat gcggtctgtt cttttatact gacagatgtc tcctgcaggg ctttggcaga
     1981 tcgctcgatg atggtttcca catccctgac tgaagcggtt atttcttcgt tagaaaccga
     2041 ctgctcttcc gatgcggctg caatttcatg gaacatgatc gcggtatttc ctgatgcttc
     2101 cgaaatattg tttatagatg atactgtctt ttcaattaaa ccttcaccct ctacaacaac
     2161 ttttgttccc cttcccatac tttccacggc attcttagtt ttttcctgaa tatctttgat
     2221 caactcgttg gccctgtctg ctgctgtttt tgattcctcg gcaagttttc gcacttcatc
     2281 tgcgactata gcaaaacctt taccgtgctc gcctgcacgt gccgcctcaa tcgaagcatt
     2341 aagagcaagc atatttgtct ggtaggcaat ttttgctatt gatcttgtgg tttcaccaat
     2401 tttttgggat tttatgtcca gttctaatat tagatctgat gaattcccaa ctgcttttct
     2461 aatttcaccc attaaccgga ataattcatg ggacattatg ttgatttctt tagccgtctt
     2521 atccgcttca tctgctcttt cagcggcctt ttgcgaatct gatgcaaca
//
LOCUS       DATGBN010000295         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_178617, whole genome shotgun sequence.
ACCESSION   DATGBN010000295 DATGBN010000000
VERSION     DATGBN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178617"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..600
                     /locus_tag="VKL21_07160"
     CDS             121..600
                     /locus_tag="VKL21_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45583.1"
                     /translation="MNDYWGVPEEEYEISLMQFARQQLSDSGYSVLEEKVEVCSGAGA
                     LTLTAIIKKGRELMGLYIHDGPRQRKQRGSPIIRVLGLLPEIKNVENLRKFVIMHQDG
                     FYIILEHENTILRDKAQVDLLQIYSNLYDSKIRDLYETDLSSAADVLRVRLRKESQA"
     gene            complement(578..1402)
                     /locus_tag="VKL21_07165"
     CDS             complement(578..1402)
                     /locus_tag="VKL21_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45584.1"
                     /translation="MKEIASHNDVNIYAPDNSRFSFMKSPYAAHITNSAVDIYSGCFG
                     GEALSPVDGRIIDIRSFDTPTPFKGKDSKEYLIAIQQGKYVIKIIHIKPDVLIGDEIS
                     KGDRIGTFIKNGYFIFWNDAVMHVEVRDFCDYLRASNNFQLSPRIEWRSLPEGRISEF
                     DCVVEDVNESYSLLLAPYLTCGEIGGFPIDAGFIDGYISSDKDGHFFGILKPQGFFRP
                     EVSFLEISKENKPVRVSGIAFCLSFNEPRIKLIPEKYGDKPLNPGDKIHIKLGILS"
     gene            complement(1403..>2350)
                     /gene="glyS"
                     /locus_tag="VKL21_07170"
     CDS             complement(1403..>2350)
                     /gene="glyS"
                     /locus_tag="VKL21_07170"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMB45585.1"
                     /translation="YCLALTQKFLIRVGVSPEKMRFRQHMLDEMAHYAADCWDAEILS
                     DRFGWVEVVGIADRTDFDLKAHAKQSQKELSVYVAYDSPKKVLKFVVKPDMGVLGPMF
                     KGKAANVANALKALKSEELSGDVIEVTVDGEKIKINKESVKFETITEEIHGENIIPHV
                     IEPSFGIDRITYSLLEHSYFEEEAKEKIEGEEEENGELRIVLRLPPEVAPVQAAVLPL
                     LSRDELIKPGTEIVNKLRKAGILVAFDDSGTIGRRYRRNDEIGTPFSITVDYQTLDDG
                     TVTIRDRDTMKQVRASISDIENNVYDLIYRGKSFENAGV"
BASE COUNT          655 a          534 c          447 g          714 t
ORIGIN      
        1 tgtgcctccg aatttcatca ctaatctcat ataggcgttt tcaatcgtca gaggttctat
       61 taaagcttac taaaaacctc aaagatacct ataaatatcc atcaatacct gcttgttgtc
      121 atgaacgatt actggggcgt ccctgaagag gaatatgaga tttcccttat gcagttcgca
      181 agacaacaat tatccgacag tggttattct gtattggagg aaaaagtgga agtatgcagc
      241 ggcgcagggg ctctcacttt aactgcaata ataaaaaaag gccgtgaact tatgggatta
      301 tatattcacg atggccccag gcagcgaaaa caaagaggct cccccataat cagggtgctt
      361 gggttattgc cggaaataaa gaatgtggaa aacctcagaa agttcgtaat catgcaccag
      421 gatggttttt atattattct ggagcatgaa aatacaattt taagagacaa agcacaggta
      481 gatcttcttc aaatctactc taacctatat gattcaaaaa taagggatct ttatgaaacc
      541 gacctgagca gcgcagcaga tgtattgagg gtgagactca ggaaagaatc ccaagcttga
      601 tatggatttt atcacctgga tttaatggtt tgtcaccata tttttccggg atcaatttta
      661 ttctgggctc attaaaagac aaacaaaagg caatccccga aactcttacc ggcttatttt
      721 cttttgatat ttcaagaaaa gagacttccg gacgaaaaaa tccttgcggt ttgagaatgc
      781 caaagaaatg accgtcttta tcggaactga tatagccgtc aataaatccg gcatctattg
      841 ggaatccccc aatttccccg caggtcaggt aaggagccaa aagcaaagaa taactctcat
      901 ttacgtcttc aacaacacaa tcaaattctg atatcctgcc ctcaggaaga cttctccatt
      961 caatcctcgg tgaaagttgg aaattatttg atgcgcgcag ataatcacag aagtccctga
     1021 cctcgacatg catgactgcg tcgttccaga aaataaaata tccattcttg ataaatgtcc
     1081 ctatacgatc tccttttgat atttcatcac ctatcaagac atccggttta atatgaatta
     1141 ttttgatgac atattttccc tgttgtatcg ctattagata ttctttggaa tcctttcctt
     1201 taaaaggcgt aggtgtgtca aaactcctga tatcgattat cctaccatcg acgggagaga
     1261 gcgcctcgcc cccgaagcac ccggaataaa tgtcaaccgc actatttgta atatgcgctg
     1321 cataagggct tttcatgaaa gaaaatcttg aattatcggg tgcataaata tttacgtcat
     1381 tgtgacttgc aatttctttc atttaaactc cggcattttc gaatgatttt cccctgtata
     1441 tcagatcgta aacattgttt tcaatatcgc tgatggatgc gcgcacctgc ttcatagtat
     1501 ccctgtccct gatcgtcacc gtaccgtcat caagcgtctg gtaatccaca gttatggaaa
     1561 atggcgtacc tatctcatca tttcggcgat atcgccttcc aatagttccg gaatcatcaa
     1621 aagccacgag tattccggct ttcctgagtt tgtttacaat ttcagttccc ggctttatca
     1681 attcatcccg tgaaagaaga ggcaaaaccg ctgcctgcac aggcgccact tcggggggga
     1741 ggcgcaatac tattcttaat tctccatttt cttcttcttc accttctatt ttttcttttg
     1801 cctcttcttc aaaatatgaa tgttcaagta gagaataagt gatcctgtct atcccgaatg
     1861 atggctctat gacgtgcggt attatatttt ctccgtgaat ttcctctgtt atggtttcga
     1921 atttcacgga ttctttattg atcttaatct tttcgccgtc aactgtcact tctatgacat
     1981 ctcctgagag ttcttcggat tttaatgctt tcagggcatt tgccacattt gcggccttcc
     2041 ctttgaacat tggaccaaga actcccatgt cgggtttgac cacgaacttc aggacttttt
     2101 tgggagaatc gtatgcaaca tatacgctta attccttctg gctctgtttt gcatgtgctt
     2161 taaggtcaaa atcagtcctg tccgcgatcc ctacaacttc cacccacccg aacctgtcac
     2221 tgaggatctc agcgtcccag caatctgcag catagtgagc catttcatcc agcatgtgct
     2281 gcctgaatcg cattttttca ggagacacgc ccactcgtat gaggaatttt tgtgtcaagg
     2341 caagacaata
//
LOCUS       DATGBN010000296         5278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_411694, whole genome shotgun sequence.
ACCESSION   DATGBN010000296 DATGBN010000000
VERSION     DATGBN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411694"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1246
                     /gene="pdxS"
                     /locus_tag="VKL21_07175"
     CDS             350..1246
                     /gene="pdxS"
                     /locus_tag="VKL21_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HMB45586.1"
                     /translation="MQLENLRHGTELLKRGFAKMQKGGVIMDVTTPEEAHIAEKAGAV
                     AVMALHQVPADIRKAGGVARMADPQVIAAIIESVTIPVMAKARIGHFVEAEIIEALGV
                     DMVDESEVLTPADEMYHIDKTKYTIPFVCGARDLGEALRRINEGAAMIRTKGEAGTGD
                     VKEAVRHMRAIMGVVRELKGKSAEELIAVARKIEAPIELVKETAQLQRLPVVNFAAGG
                     IATPADAALMMRLGADGVFVGSGIFKAENPEKMACAIVEAVNNYDNPSELARISKGLG
                     GAMKGIDIRSLPDKEILQGRGW"
     gene            complement(1269..2834)
                     /locus_tag="VKL21_07180"
     CDS             complement(1269..2834)
                     /locus_tag="VKL21_07180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMB45587.1"
                     /translation="MELEGNKIIRILKNNLVVILVLVGISARIFLFGNVPPGLNQDEA
                     SMGYDAFSLLNYGMDRNGFHYPVYLVAWGSGQSVLYAYFTMPFIYLLGLNVVSIRLVN
                     LIFGIVSLIIFYLLVQKVTNRPVALLSLFLLAISPWHVMMSRWALDANLFPSIFLIAV
                     FLLASSFEDKRILPASLFFFALSFYAYATSFFIVPVFLLIVFSYLMYHKKIDIKTLPT
                     GLFIFTITALPIFLFVVINHFKISSINAVLFSIPRLTGTPRFFSLSSLFSSDFLSTSI
                     DNFINVIKIIVTQNDGLIWNSIPEYGFIYLFSLPFLIIGFISLVRKNIALKKFEKSFL
                     FLAWLMVSFLLGSLTNPNINRINTIFIPLIFITAMGINYITRNIKSLYLPIIILYLIS
                     FSMFSFNYFTVYPKETEQIFFESFGEAINFASEKQDSRIYVTEQVNMPYVYVLFYQKI
                     NPEIFNSSVKYYNPGQNFQLVKSFDRYEFGIKDINKLENAVYVIHNSEEGKFDQNDFV
                     FKKFKYYSVVTRK"
     gene            complement(2825..3532)
                     /locus_tag="VKL21_07185"
     CDS             complement(2825..3532)
                     /locus_tag="VKL21_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB45588.1"
                     /translation="MIDEKIENSKDISIIIPAYNEEYRIRNVINKYCGFFTDSEFIIV
                     CDGIDNTKKIVSELMVCYPHLKLLEFNEKLGKGRAIIEGMRTAKHDKVGFVDADESIE
                     PDDLKRMFDALLRADCVIASRRLKDSRIPVKQPLKRRISSRVFNIIVSIIFGLNIKDT
                     QCGAKVFRKEVIRGVLNNIKTKGFEFDVELLWRLKKKGYNIAEFPVTWKHSPGSSFGL
                     GNAPMMLLSLLKVRLWN"
     gene            complement(3534..5276)
                     /locus_tag="VKL21_07190"
     CDS             complement(3534..5276)
                     /locus_tag="VKL21_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HMB45589.1"
                     /translation="MLVTGDAYVDHPSFGTALIGRVLWDAGFSVGIITQPDWKSRDDF
                     KKFGKPRLFFGVTSGNVDSMVNNYTANLKIRNDDVYSPGGKGGLRPNRAAIVYSDKLH
                     SIFPDTPIVLGGIEASLRRFAHYDYWSDSVRQSILADAPADILVFGMGERQVVEIADR
                     LSKGEKIKEITDIAGTAIKMEISKWRFLRHEEYVEIPGFSDVSRDKELYARAFNLHYQ
                     EQDPIRGRPVVQPHPKTIIIQNKPARPLCACELDHVYELPFSRMAHPSYNKPIPALAP
                     VKFSIVSHRGCFASCSFCALTHHQGRIVQSRSIGSIVREVKRMTKMRDFKGIVQDVGG
                     PTANMYSLYCKRWEEDGACADKICMGCKSLGTSHKEQVELLRTLLKIEGIKKVFIGSG
                     IRYDLALRDSSGYISELCEHHVSGHLKVAPEHIIKHITDIMHKPGKEVFEEFRKRFDV
                     ENKRLGKRQYILPYFMSGHPGCTVEDMVELAEYIRDNNLYTEQVQDFTPTPMTVSTCM
                     YYTGIDPFPMKEVHVAKGREKMIQRALLQYREKRNYGFVVEGLKMAGREDLIGNGRKC
                     LIEGRTKKIKSERF"
BASE COUNT         1638 a         1090 c          980 g         1570 t
ORIGIN      
        1 tgatgcaata atatcaggtg agatttcctg gaatgaatct ttgagatttg atagaacttc
       61 ctctcgttgt tgtattctaa atctagaaga accaattaaa tcgccagtta gaaccgcata
      121 tatttttcca gtttccataa agataccacg tgacaatatt ataaggttac aaccttataa
      181 tatcatatta taagtctatg tccttatatt aacctcttca ggtagaaaga tttaatcttt
      241 ccttgtagag aggctaaaag tttatcgaac ccctcatatt agtccatgtc aaaggtgatc
      301 ttatcaatgg cgcaaagtat atctcatata attaagcagt taagacgtta tgcaacttga
      361 aaatctcaga catggcactg aacttttaaa acgcggtttt gcaaagatgc aaaaaggcgg
      421 agtcattatg gatgtcacaa cacccgaaga agcgcatatt gcagaaaaag caggggctgt
      481 ggctgttatg gcgctgcacc aggttcctgc ggatataaga aaagcaggag gagttgccag
      541 gatggctgat ccgcaggtta ttgcggcaat tattgaatct gtgaccatcc ctgtgatggc
      601 taaggcaagg atagggcatt tcgttgaggc cgagataatt gaagcgctcg gcgttgacat
      661 ggtagatgag tcagaagtcc tcacgccggc agatgagatg tatcatattg ataaaacaaa
      721 atacacaata ccatttgttt gcggagcacg cgacctcgga gaggcgcttc gaaggatcaa
      781 cgaaggcgcc gctatgataa ggacaaaagg cgaagcaggt actggcgatg tgaaagaggc
      841 agtacggcat atgcgtgcta ttatgggcgt tgtgcgggag ttaaaaggca aaagtgcaga
      901 ggaattgata gcagtggcaa gaaaaattga agcccctata gagcttgtaa aagaaacggc
      961 acagcttcag aggcttcctg tcgtgaactt tgcagcaggt ggaatagcca caccagcaga
     1021 tgcggctctc atgatgcgtc ttggggcgga tggtgttttt gtgggctcag gaatatttaa
     1081 ggcggaaaat cccgaaaaga tggcctgcgc aatcgtcgaa gctgtgaata attatgataa
     1141 tccttccgag cttgcccgga tatcaaaggg ccttggcggt gcgatgaaag ggatagatat
     1201 aaggagttta cctgataagg aaattctgca gggaagagga tggtaagtct tccaaaaagg
     1261 ctttataatt atttccttgt taccacagaa tagtacttaa atttcttgaa aacaaaatca
     1321 ttctgatcga attttccttc ttcagagtta tgtattacat atacggcatt ttcaagttta
     1381 tttatatctt taatcccaaa ttcatatctg tcgaatgatt taactaattg aaaattttgt
     1441 cctggattat agtattttac agaactatta aatatttcag gattgatttt ctgataaaac
     1501 aatacgtata cataaggcat atttacctgc tctgtcacat atatccttga atcttgtttt
     1561 tctgatgcaa aattaattgc ttctccaaaa gattcaaaaa aaatttgttc cgtctctttt
     1621 ggatatactg taaagtagtt gaaagaaaac atagaaaatg atattaaata caatattatt
     1681 ataggcaaat aaagcgattt aatatttctt gtgatataat taatacccat agctgtaatg
     1741 aaaattaatg gaataaatat agtatttatt ctattgatat tagggtttgt taaggatcct
     1801 aatagaaaag aaaccattaa ccatgccaga aaaagaaaac ttttttcaaa tttttttaag
     1861 gctatgttct tccggaccag gcttataaaa cctattatta agaatggcag actgaataaa
     1921 tatataaaac catattctgg tattgaattc cagatcaaac catcattctg agtgactatt
     1981 attttaataa catttataaa attatcaatg gaagtgctca aaaaatcact actaaacaac
     2041 gaagaaagtg aaaaaaatct tggagtgcct gttaatctcg gaatggaaaa taagactgca
     2101 tttatcgaac ttatcttaaa atgattaata actacaaaca gaaaaatagg cagcgctgta
     2161 attgtaaaaa tgaacagtcc tgtaggcagg gttttgatat ctattttttt atggtacatc
     2221 aagtatgaaa aaacgatcaa tagaaaaaca ggaacaataa aaaacgacgt agcatatgca
     2281 taaaaagaca atgcaaaaaa gaataatgag gctggtagga tacgtttatc ttcaaatgat
     2341 gatgctaata aaaaaactgc aattaaaaaa attgatggaa atagatttgc atccagcgcc
     2401 caccttgaca tcatcacatg ccagggacta atcgccagta aaaatagaga taataatgcc
     2461 actggtctgt tagtaacttt ctgtaccagt aaataaaata taataagaga tactattcca
     2521 aatattaagt taaccaacct gatagataca acattcagcc ctaataaata aataaacggc
     2581 atcgtgaaat aagcatataa tacactctga ccactacccc atgcaacaag atataccgga
     2641 tagtgaaatc catttctgtc cattccataa tttaagagtg aaaatgcatc atatcccatt
     2701 gatgcttcat cctgatttaa tcccggtgga acgttcccaa ataaaaaaat acgcgcgcta
     2761 atacccacta acaccagtat tacaactaaa ttgtttttca atattcgaat tattttatta
     2821 ccttctagtt ccataatctg accttcaata aagagagaag catcatggga gcatttccta
     2881 acccaaaact tgaacctggt gaatgcttcc atgtgacggg aaattctgcg atattatatc
     2941 ctttcttttt caatctccaa agaagctcaa catcaaattc aaatcccttt gtcttaatat
     3001 tgttcaggac cccccttatc acttcctttc tgaaaacttt agccccgcat tgtgtatctt
     3061 taatatttaa tccgaaaatt atactcacaa taatattgaa aaccctgctt gaaatccttc
     3121 tcttaagagg ttgttttaca ggaatccttg aatctttcaa tctcctggat gcaatcacac
     3181 aatcagctct cagtaatgca tcaaacatcc tctttaaatc atctggttca atagactcat
     3241 ccgcatcaac aaatccaact ttatcatgtt tcgcagttct cataccttct attatggcgc
     3301 gtccctttcc aagtttttcg ttaaattcta ataatttcag atgaggataa cacaccataa
     3361 gttcgctgac aatttttttt gtattatcta taccgtcaca aactatgata aactctgaat
     3421 cagtgaaaaa accacaatat ttgtttatga catttctaat tctatattct tcattgtatg
     3481 ctggaattat aatagaaatg tccttagaat tttctatctt ttcatcaata atattaaaac
     3541 ctctcggatt ttatcttttt tgttctcccc tctataaggc acttccttcc attcccgatc
     3601 aaatcctccc tcccggccat ctttagcccc tcaaccacaa acccataatt cctcttttcc
     3661 ctgtactgca agagcgcccg ctggatcatt ttttcccgac cttttgccac atgaacttct
     3721 ttcatcggga aaggatcaat accagtataa tacatgcacg tcgaaactgt catgggcgtg
     3781 ggtgtgaaat cctgcacctg ctctgtgtaa aggttgttat cacgaatata ttctgcaagt
     3841 tctaccatgt cttccactgt gcatccgggg tggcctgaca tgaaataggg aagaatatat
     3901 tgtcgttttc caagcctttt attttcgacg tcaaatcttt tcctgaattc ctcgaacact
     3961 tcctttcccg gtttgtgcat gatatccgtg atatgtttta ttatgtgttc cggtgcaact
     4021 ttgaggtgac cgctaacatg atgctcacat aattcagaaa tgtaaccgga tgaatcccgc
     4081 agcgcaaggt cgtagcgtat gcctgaacct atgaacactt tcttaatccc ttctatctta
     4141 agtagggtgc ggaggagttc gacctgttcc ttgtggcttg tacctagact tttgcaaccc
     4201 atgcatattt tatcagcaca tgcgccatct tcctcccatc gcttgcagta cagcgagtac
     4261 atatttgccg taggtccccc cacatcctgc acgatgcctt tgaaatcgcg catcttggtc
     4321 attcttttta cttcccgcac aatagacccg atgctcctgc tctgcacgat ccttccctgg
     4381 tgatgggtca gggcgcaaaa agagcatgaa gcaaaacatc cgcggtggct cacgatcgag
     4441 aattttacgg gagcaagcgc cgggatgggc ttattatatg aaggatgcgc catcctcgaa
     4501 aagggaagtt cgtagacatg gtccagttca caagcgcaca ggggccttgc aggtttattc
     4561 tggattatta tggttttcgg atgcggctgc accacaggtc ttccccggat aggatcttgc
     4621 tcctgataat ggagattaaa tgccctggca taaagctctt tatcgcgcga aacatcagaa
     4681 aatcccggta tttccacata ttcctcatgc ctcaggaaac gccatttgct tatttccatt
     4741 ttaatagcgg tgccagcaat atctgttatt tctttgatct tttcaccttt tgaaagacga
     4801 tctgctatct ccacgacctg tctttccccc atcccaaaaa caagtatatc tgcaggcgca
     4861 tcggcaagta tggactggcg cacagaatcg gaccagtaat cataatgggc aaaacgccgc
     4921 aggcttgctt caattccccc gagcacaatg ggtgtatccg ggaaaatgga atggagctta
     4981 tcagaatata ctattgcagc acggtttggc cgaagtcctc ctttcccccc cggtgaataa
     5041 acatcatcat ttcgtatttt caggtttgca gtataattat ttaccatgga atctacattc
     5101 ccggaagtga caccgaagaa caatcgtggt tttccaaact tcttgaaatc atccctgctt
     5161 ttccagtcgg gttgtgttat gatcccaaca ctaaaacccg catcccagag aacacgtcct
     5221 atcaatgctg tcccgaagga ggggtgatcc acatatgcgt cacctgttac gaggatta
//
LOCUS       DATGBN010000297         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_372965, whole genome shotgun sequence.
ACCESSION   DATGBN010000297 DATGBN010000000
VERSION     DATGBN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372965"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VKL21_07195"
     CDS             <1..248
                     /locus_tag="VKL21_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HMB45590.1"
                     /translation="IKEIPLTNAVNLAFFMVNISNILINCVRPQNAEFSALDIKAHFR
                     GIKYVDEVLKLLPKKLDPILIQQIFKEITSIGKINTD"
     gene            304..510
                     /locus_tag="VKL21_07200"
     CDS             304..510
                     /locus_tag="VKL21_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45591.1"
                     /translation="MRLVSEIQTTNFLALTSELFSKSKIINLPEKAEYSFQDTLNVLL
                     HAATSTTNTFIAGVVATLLHPGAT"
     gene            1122..1286
                     /locus_tag="VKL21_07205"
     CDS             1122..1286
                     /locus_tag="VKL21_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45592.1"
                     /translation="MKLKGLRTPEIWTLINNALTQFKEGITGNPEALADDREFYERVI
                     EASKTGLIEL"
     gene            complement(1436..>2089)
                     /locus_tag="VKL21_07210"
     CDS             complement(1436..>2089)
                     /locus_tag="VKL21_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISH6 family transposase"
                     /protein_id="HMB45593.1"
                     /translation="GTEIKSVKAVTEKTVYCGDAEKEMKNAMTEDADSYQLDLIHAAR
                     IAGFKLWEDKIMSLEERNRIVSKLEGVLYTLKNSVEKHLIDKNTDDLKNRINMTVDDL
                     KKMSKELWKLGCRKTATFIKKYSNSMVVFAKFAMNGIRVPWNSNIIERLMGEISKRTK
                     HKWMKWTTKGLETILNIILVRYCSEENYEEFKNRIIMSENLIFIMAEVKICSFRGEF"
BASE COUNT          714 a          377 c          348 g          650 t
ORIGIN      
        1 acatcaaaga aatacctctc acgaatgcag taaaccttgc tttttttatg gtaaatattt
       61 ctaatatttt aatcaactgt gttcgtcccc aaaatgcaga atttagtgct ctggatataa
      121 aagcacattt tagaggcatt aaatatgtag atgaagtatt aaaattgctt ccaaaaaaac
      181 ttgatccaat tttaattcag caaatattta aagaaataac gtcaataggt aaaattaata
      241 ctgattaatg gcgaaggtat tgatgtatat tatcccatct aaaaatatag aggggataat
      301 aaaatgaggc ttgtatctga aatacaaaca accaatttct tggctttaac ctcagaacta
      361 tttagtaagt caaaaataat aaatctacct gaaaaagctg agtacagttt tcaagacaca
      421 ttgaacgtat tgctgcatgc agccacttcg actacaaata ccttcatagc gggggtagta
      481 gcgacactgc tgcatcctgg ggccacgtaa acccattatg taaattttca acttattcaa
      541 tcgtatggca gaggcggaga aatcgcggta aacaacagag aagatcaaga aatagccagt
      601 ttaaccttgc atctattgca aatttgtatg gtatacgtca atacactact cgtgcaaaaa
      661 gtgttatctg agaaagaatg gttgaataaa atgcaaccgg aagatttccg tgggctgaca
      721 cccctttttt atggccacat caccccatac ggcgagttcc aattagatat gaataaacga
      781 ataaatattg aagaaaccat ctaaattatc aagtggcccc aaaatgcaac agcgttgcta
      841 ctaccccatg atgaaataaa tgagaggttg tactaatctc ttttcaaagg aggaaaacat
      901 gacaatagaa actcaaaaga aacactctga tctcgaaagg atgacgaatc gacaaataat
      961 cattagaatt gatcccgaaa cgaagatgga aatgtatata tatccaaacg tacaatgata
     1021 tatatatata tatatataat gtacaaaagg gcaataagcc tgatgtaaat taagagggtt
     1081 aggaaataaa ggtttcttag ccctatattg ttgcaggcaa tatgaaattg aaaggtctta
     1141 ggacacccga aatatggact ctgataaaca atgcactgac tcaatttaaa gagggaataa
     1201 cagggaatcc agaggcttta gcggatgaca gggaattcta tgagagggta attgaagcca
     1261 gtaagacagg gttaattgag ctttaaaaat actctcttct cttttttttt gacaaaaagg
     1321 aatatataaa acatcctcat ctaagaagca aagtgataat tgagtaatcc actatcatct
     1381 tgaataagtg gaaactatgt taagtgggaa aatattggtc gtgtctaaag atcggctaaa
     1441 attctcctct gaatgaacat attttgactt ctgccattat aaagattagg ttttctgaca
     1501 taatgattct atttttaaac tcctcataat tctcttcaga acaatatctc acaaggatga
     1561 tattcagaat tgtttcaagg cctttcgtag tccatttcat ccatttgtgt ttagttcgtt
     1621 ttgaaatttc tcccataagt cgctcgataa tatttgaatt ccaaggaact cttatgccat
     1681 tcatcgcaaa tttagcaaaa accaccatgc tgttgctata tttttttata aacgttgcag
     1741 tttttctgca tccaagtttc cataactctt ttgacatttt ctttaaatca tcaactgtca
     1801 tgttgattct gttctttaga tcatcagtat ttttgtcaat cagatgcttt tcaaccgagt
     1861 ttttcaatgt gtagagaact ccttcaagtt tgctaacaat tctatttcgc tcctcaagtg
     1921 acatgatttt atcctcccat agtttaaaac ctgcaatcct ggcagcatga ataagatcca
     1981 attgataaga gtcggcatct tcagtcatcg cattcttcat ttctttttcc gcatccccac
     2041 aatagacagt cttttctgta actgctttta cactcttgat ctctgttcc
//
LOCUS       DATGBN010000298         5563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_528034, whole genome shotgun sequence.
ACCESSION   DATGBN010000298 DATGBN010000000
VERSION     DATGBN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528034"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VKL21_07215"
     CDS             <1..498
                     /locus_tag="VKL21_07215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMB45594.1"
                     /translation="PNTLTLFGLLLSLVSAYFFARWELKMAGAFLLAGGFFDALDGLV
                     ARENNRMTKFGGFLDSVCDRFADAAVLLGAIYGGLSQGYSNQYWLTGTLAIIGSLMVS
                     YTRARAEAAGASASVGFGERPVRMIIIILGAFLDMVYWAILFVAVISFITVFQRIAFV
                     RRLLK"
     gene            551..1630
                     /locus_tag="VKL21_07220"
     CDS             551..1630
                     /locus_tag="VKL21_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HMB45595.1"
                     /translation="MAGIRIAIVGVGNCASSLIQGLEYYKNIKNSDDLVPGLMHNSLG
                     GYLISDIRPVAAFDIDMRKVGTDLSEAIFSDPNCTKKFADVPDYDIKVMKGQVLDGVA
                     PHMKDYFRVDDKQKPVDVASVLRKSKADILINYLPVGSEEAVKWYATQAIEAGCGFIN
                     CIPVFIASNKQWAEKFKKAKLPIIGDDIKSLVGATIVHRALTQTIVDRGARIDSTYQL
                     NIGGNTDFRNMTDQSRLKSKKISKTESVTSQIPYDAYVYAGPNGCIDCLNDNKVCHLK
                     IDFKLFGDVPAYIDLKLSVEDSPNSAGVVVDAIRVAKIALDRKIGGPILPASAYFMKH
                     PPQQMRDIEAREKLEEFIKLDKVKD"
     gene            complement(1685..4033)
                     /locus_tag="VKL21_07225"
     CDS             complement(1685..4033)
                     /locus_tag="VKL21_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMB45596.1"
                     /translation="MQEKENKFNNINKVSLTGISRLFFVWGISLLFIQFLWIPALAQN
                     AEYQDGYNLIYYDNGSKEVINGALNFLRYDGAWRPNEELNISNGSWPYLYSQNTTSAN
                     FKVDDTTLSIPKANTVFKTNLNSISYELKYTKSELQGKGISINKNSEFIDIPFHLKSR
                     KPKKEFKDRNNIKYGRFHFKAGMEHIVVHDDTPRDYIEDGKVFQDVTYLFQPDYEFKI
                     ENGTIQLVFNNNSLDKLTGNVIVEIRTWDIIGANNWGGNVTFSQTTDVKATGNAELKQ
                     TVADYSLYTRFDEGKGKTIHNENPLNVRLGDLLGAVGTGTYTAGKYGQAIYFNGIDNK
                     ILFKDDPAFRLAGDFTISYYIKLTSDVSNVDSDITRKGSTATANPDSWWKMELTNNLM
                     HGSVTKNGATAVESYDTVDRRDGNWHFVAYTRGGTTCSLIVDGTTVKTTSNCPTNAVN
                     TALLSIGAKDTYVQTTGLDFTRGSIDEVRIFNRKLSASELTAIQNNAHNTAGTITRNL
                     VSLIKTGEEIKELGCYGTWDSSITKVDVMASADNSNWNMIKSIAAPNVNYPVNAGNNY
                     KYSRCSLSTTDSSNTPIIQSIRANIGPKGANPIIAEAGGPYSGTPGVLINFIGSASGG
                     SAPYLYKWNFGDGGTSSLANPAHAYTTPGPYTATLTVKDNAGITSSPDTAPVSVASLN
                     VAGSISGYKINDINGNGKWNAGESGISGWKIKLVGKNIKKEILTDALGFYIFDNLPAG
                     KYTVSEENKKGWKHTSSTSRKIKLKNGMKSINNIFTNKMKKR"
     gene            complement(4640..>5563)
                     /locus_tag="VKL21_07230"
     CDS             complement(4640..>5563)
                     /locus_tag="VKL21_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMB45597.1"
                     /translation="QLVSKIARTIGRALRLNEDLIEAISLGHDIGHVPYGHLGEKILS
                     KLCEEHKIGVFLHNVQSVHFLYDIEDCDLTLQVLDGILCHDGEVHNQSLKPSGNADWD
                     TFDSKMKDIKAQTGKYFPSTMEGCVVRVADTIAYLGRDIKDAVEVKLICENLPNFPEK
                     CKKFFEYEPGKEDINWAILDTLIKDIINNSFGKDNLSFSDNASECIHVCKEYNYKYIY
                     NSCSLLKEKRKIKKMYTILFDEFLKDLNEENEASLIYSDMINLKWISSDYKEKATPAE
                     KVRDYLAGMTDRYFEWVFKKITIPERVTRNF"
BASE COUNT         1571 a         1102 c         1007 g         1883 t
ORIGIN      
        1 ccgaatacat tgaccctttt tggacttctg ttaagtcttg tctccgctta tttttttgcg
       61 cggtgggaac ttaagatggc aggagctttt cttcttgcag gcggtttttt tgatgccctt
      121 gatgggcttg tagcgcgcga aaataaccgg atgacaaagt tcggaggttt tcttgattct
      181 gtatgcgaca ggttcgctga tgctgctgtt ttattggggg cgatatacgg gggtctgtcc
      241 cagggatatt ctaatcaata ctggctgaca ggtacccttg cgattatcgg ctcgctgatg
      301 gtcagttata cgcgcgctcg cgccgaagca gctggagcga gcgcttctgt tggctttgga
      361 gaaaggccgg ttagaatgat aataataatt ttgggcgctt tccttgatat ggtatattgg
      421 gctatccttt tcgtagcagt aatatctttc ataacggttt tccagaggat agcattcgta
      481 agaagattat tgaaataaca aattttaagt tgtataagga taactataag cgggtataag
      541 aggaaattta atggcaggca tacgtattgc aatagttggt gtagggaatt gtgcatcatc
      601 tttaattcag ggacttgaat attataaaaa cataaaaaat tctgatgacc ttgttccagg
      661 attaatgcat aattcactgg gaggttatct catctcggat ataagacccg tagcggcttt
      721 tgacatcgat atgcgaaaag tgggaactga cctgtccgaa gcgatatttt ctgatccaaa
      781 ctgtacgaaa aaatttgctg atgtcccgga ttatgatatc aaggtcatga aaggacaggt
      841 gcttgatggg gttgctcctc atatgaagga ttatttcagg gtggatgata aacaaaaacc
      901 tgttgatgtg gcttccgtat taagaaaatc aaaagcggac atactgatca attatttgcc
      961 tgtgggttcg gaagaagcgg ttaaatggta tgcaacacag gccattgaag cagggtgcgg
     1021 cttcattaat tgtatccccg tctttatagc ttcaaataag caatgggctg aaaaattcaa
     1081 aaaagctaaa cttccgataa tcggcgacga tattaagagc cttgttggtg caaccatagt
     1141 tcacagggcg cttacccaga cgatagttga caggggtgca aggatagaca gcacctacca
     1201 gttgaatata ggagggaaca cggattttcg gaacatgacc gaccaatcaa ggcttaaatc
     1261 caagaagatc tcaaaaacag aatccgtaac ttcccagatt ccctatgatg cttatgttta
     1321 cgcaggtccg aacggctgca tagattgcct gaatgataat aaagtctgcc acctgaaaat
     1381 tgatttcaaa ctattcgggg atgtcccggc ttacatcgac cttaaactat ccgtcgagga
     1441 cagccccaac agcgcaggtg tggtggtaga tgcaatccgc gtggctaaaa tcgctcttga
     1501 taggaagatt ggcggaccga tacttccagc ctccgcttat ttcatgaagc acccaccgca
     1561 gcaaatgcgt gatattgagg cacgggaaaa actcgaggaa tttattaagt tagataaagt
     1621 taaggattaa tatattttat tccaaaaaat acaagcaaaa gtatcattcg acaggattta
     1681 cttatcacct ttttttcatc ttgtttgtga aaatattatt aattgatttc atgccatttt
     1741 tcaatttaat tttcctggat gtagaacttg tatgcttcca gccctttttg ttttcttcgc
     1801 ttacggtata tttacctgca ggaaggttat caaatatata aaatcccaga gcatcagtga
     1861 gtatttcttt tttgatattt ttgccgacca gtttgatctt ccaacccgat ataccgcttt
     1921 ctccagcatt ccatttgcca ttcccgttaa tatcatttat cttgtatcct gagatactac
     1981 ctgcaacatt taaggatgca actgaaacag gagcagtatc cggagaagat gttattccgg
     2041 cattatcttt cactgtcaaa gtagcagtat aaggtccagg ggttgtgtat gcatgagcgg
     2101 ggttggcaag gctgcttgtc ccaccatctc caaagttcca tttatacaaa tatggcgcac
     2161 tccctcctga agcggaacct atgaaattta tcaaaacacc gggagttccg gaatacggcc
     2221 ctcctgcttc ggcaattatt ggattggctc cttttggacc tatatttgcc ctgatacttt
     2281 gaattatcgg ggtatttgaa gagtctgttg tacttagcga acatcttgaa tatttataat
     2341 tgttcccagc attaacagga taattaacat ttggagctgc gattgatttt atcatattcc
     2401 aattgctatt atcggcgctt gccataacat ccactttcgt tattgagcta tcccatgttc
     2461 catagcatcc caactctttt atttcctctc ctgttttgat taaggaaaca aggttacgag
     2521 tgattgtacc tgctgtgtta tgtgcattat tctgtatagc tgtaagttcc gaagctgata
     2581 atttacgatt gaatattctt acctcatcta tgctcccgcg ggtaaaatcc aatcctgtcg
     2641 tttgcacata ggtatccttc gcccctatgc tcaacaaagc agtgtttacg gcatttgtgg
     2701 ggcagttgct tgttgttttt acagtagtgc cgtctactat caaactgcat gtggttcctc
     2761 ccctggtata tgcaacaaaa tgccagtttc catccctgcg atccaccgta tcgtacgact
     2821 caactgccgt agcgccattt tttgttacag agccgtgcat taaattattt gtaagctcca
     2881 ttttccacca tgagtccgga ttcgcagtgg cagtgctgcc ttttcttgtt atatccgaat
     2941 caacattgct cacatctgaa gtcaatttaa tgtaatatga aattgtaaaa tcccccgcta
     3001 atcggaaggc aggatcatcc ttgaataaga ttttattatc aatcccatta aaataaatag
     3061 cctgcccata ttttccagct gtataggtac cggttcccac agcgcctaag agatcgccaa
     3121 ggcgaacatt taaaggattt tcattatgaa ttgtttttcc ctttccttca tcgaacctgg
     3181 tgtataatga gtaatcagca actgtttgtt tgagttcagc attgccagta gcttttacgt
     3241 ctgtagtttg ggaaaatgtt acatttccac cccaattatt ggctccaatt atgtcccatg
     3301 tcctgatctc aacaatgaca tttcccgtca atttatcgag tgagttgtta ttaaaaacca
     3361 attgaattgt accattttct atcttgaatt cataatcggg ttggaagaga tatgttacgt
     3421 cctggaatac ttttccatct tctatataat cccggggtgt gtcgtcatgt acgactatat
     3481 gttccatccc tgccttaaaa tgaaatctgc cgtatttaat attatttcta tctttaaatt
     3541 cttttttagg tttcctggat ttcaaatgaa atggaatatc aataaattcg ctatttttat
     3601 taatagatat tcctttcccc tgcaattctg attttgtata ttttagctca taactgatgg
     3661 aatttaaatt cgttttaaat acagtatttg cttttggtat actcagggta gtatcatcta
     3721 ctttgaaatt agcgctcgtt gtattttgag aatataagta aggccagctt ccattggaaa
     3781 tattcaattc ttcgtttggt ctccatgccc catcatatcg aagaaaattc aaagccccat
     3841 ttatgacttc ctttgaccca ttgtcgtaat atatcaaatt atatccatcc tgatattcgg
     3901 cattttgcgc gagcgcaggg atccagagaa attgaatgaa caaaagactt attccccata
     3961 caaaaaatag tcttgaaata ccggtcaacg atactttatt tatattgtta aatttatttt
     4021 ctttttcttg cataaccaat cacaccttta atccgaacct taaatagtat tttgatatta
     4081 aagatatatc gacaattttt atagttcttt gagttaaaca ccgattttcg tcaagtgttt
     4141 atgtttcgta attgacacat gtcaaggata tccaaaagcg atgttttttg ttgcctaaac
     4201 atggtatagc atacgatttg tgaaaaacct catttgatct ttttccaaac cattatctac
     4261 caaattagac aataaataac catcttgttg tggtaagaac gtattgaact caagactttt
     4321 taaaaactcg ttcaatctga ggttgaagtc caactccgat tttgaaaaaa gtggccctgc
     4381 tatataaact gtttttttca tatctttctt ccttttattc tctttaatat gccgattact
     4441 ctaaccatag ccacccattt atcatactat ctcattaatg tttatgtttt tgcagatttt
     4501 ctttcatcta tttacccagt gagcaggact tatcgcaaca agtatctaaa aggaaaaaaa
     4561 gaacagtatt ctgaaaagtg tcatgtttga ggtgttatgg ctgatgagat gaatattttc
     4621 tgacggactt gagcaaacat taaaaatttc ttgtgaccct ctctggaata gtaatttttt
     4681 tgaataccca ttcaaagtat ctatctgtca ttcccgctaa ataatctctc actttttcgg
     4741 ctggtgttgc tttctcttta tagtcagatg atatccactt taaatttatc atatctgaat
     4801 atatcaaaga tgcttcattt tcttcattta gatcttttag aaattcatcg aaaagaattg
     4861 tatacatctt ttttattttc cttttttctt tcaatagaga acaactatta tagatatact
     4921 tataattata ttctttacaa acatgaatac attcagaagc attatccgaa aaagataaat
     4981 tatctttacc aaagctgttg tttataatgt ctttaattaa tgtatctaat attgcccaat
     5041 ttatgtcttc tttcccaggt tcatactcaa agaatttttt gcacttttct ggaaaattag
     5101 gcaaattctc acaaataagc tttacttcca ctgcatcctt tatgtctctt cccaaatacg
     5161 caattgtgtc cgcaactcta actacacaac cctccatagt agaagggaaa tattttcctg
     5221 tttgtgcttt tatatctttc atttttgaat caaaagtatc ccaatctgca tttccacttg
     5281 gcttaagact ttgattatgc acctctccat cgtggcaaag gattccatct aacacttgaa
     5341 gtgtaaggtc acaatcttcg atgtcataaa gaaaatgtac actctgaaca ttatgcaaaa
     5401 acactcctat tttatgttcc tcgcataact tgctcagtat tttctctccc aagtgcccat
     5461 atggcacatg ccctatatca tgaccaagag aaatggcttc aattaaatcc tcgttaagtc
     5521 ttagagctct gcctattgtt cttgctattt tagaaacaag ttg
//
LOCUS       DATGBN010000299         7116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_22862, whole genome shotgun sequence.
ACCESSION   DATGBN010000299 DATGBN010000000
VERSION     DATGBN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22862"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VKL21_07235"
     CDS             complement(<1..561)
                     /locus_tag="VKL21_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HMB45598.1"
                     /translation="MATCTGCALLCDDIEAIVENNRIKETRNACRRGAARIKGCTNRL
                     IPSIDLKPTDIESSIRKTVEIIKNARSPMLFGLDNSTCEAQAKGIQLAKKINAVIDDT
                     SSFCQGLLMERILQKKLRTCSLEDIRNKADVLVYWGSDAQDSQPRHLSRFSYFPRGES
                     RQRGYEEDRMAIAIDVRESNTAKICKG"
     gene            complement(569..1027)
                     /locus_tag="VKL21_07240"
     CDS             complement(569..1027)
                     /locus_tag="VKL21_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMB45599.1"
                     /translation="MVTVMELYQLLPKTNCKKCGESTCMAFAVALLGRKKNVEECTPL
                     LEVNFKLQKEKLESLVLPSAGAEETGMIVHPELCTGCGNCVVACPVDVANDPHGAGMG
                     CAPTNEKVIFKVVDGKVMASNIKECRRFGKSRVLCYACIDPCPTGAIEFV"
     gene            1124..1405
                     /locus_tag="VKL21_07245"
     CDS             1124..1405
                     /locus_tag="VKL21_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1699 family protein"
                     /protein_id="HMB45600.1"
                     /translation="MKVVFRIIGSEEDLKDVEGKEENVHFCFRPSEKNVFELVTRTPK
                     LKRIQLPSSYQKTISNTTKALLKMKNIKLLTGDIWGHRTDIDRFAEIEV"
     gene            complement(1428..2015)
                     /locus_tag="VKL21_07250"
     CDS             complement(1428..2015)
                     /locus_tag="VKL21_07250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HMB45601.1"
                     /translation="MNIHYKNTTIKFTPWVYEPAEDSFLLTEAALSIINNSENILEVG
                     CGSGIISAVIKKNTHAKITGIDINPYAAECTRDNGVEAIRGDLLKCIKGKFDIIIFNP
                     PYLPTNDSERTTGWINIALDGGSDGRKIINRFLEDAGSNLLKNGKILMLVSSLTGIEE
                     VKSKMKSLGFYIEEKNKQRFNFEHLMVLVATKARK"
     gene            2268..4640
                     /gene="cdhA"
                     /locus_tag="VKL21_07255"
     CDS             2268..4640
                     /gene="cdhA"
                     /locus_tag="VKL21_07255"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase complex
                     subunit alpha"
                     /protein_id="HMB45602.1"
                     /translation="MTLKKGSFVIEGLENVQINIGKAGAAEEQIAEGPTPRPEIISLR
                     NWDYRLLDRYNPVYTPTGDTCDYCTYGKCDLTGNKEGACGIDLEGQSARQALMTSIMG
                     AACHSAHGRHLLNYLIKKYGEDFKIDVGPSNLQAPLTETIMGIQPKTIGDFIPVLEYV
                     EEQLTQLIAVTNTGQEGSSRDFESKALHAGMLDLLGMEVADIIQISCLGFPKSDEKAP
                     MAEIGMGILDPKKPVVICVGHNIAAPAYILDHMDKNGLFDKIEIAGLCCTAHDMTRYN
                     KSAKIIGSMSKELKYIRSGIPDVLVTDEQCVRADILKKAQKLHIPVIATNEKITYGLP
                     DRSNDSVDDIIEDLVSGKQPGVVLLDFEKIGELVPKLAMKMGPIRKAKGLTALPNDKE
                     FKKLVAKCTKCLQCTKDCPQGLPISDAMTAAVNGDFSLFETLHDKCVGCGRCDYSCPA
                     GIPILNVIEKASQRLIREEKGKMRIGRGQIGDPEIREEGRNLVLGTTPGVLAFVGCGN
                     YPDGTKDVYDIVEEMIQRSYIIISSGCAAMDIGMFKDKEGKTLYERYPGRFVKGNLLN
                     TGSCVSNAHIAATTIKVASIFAGRKTKGNWEEIADYVMNRVGAVGIAWGAYSQKAFAI
                     GTGCNRLGIPVVVGPHGTKYRRAFIGKPYKKENWNVLDGRDGSIVNVEPAPEHLMITA
                     ETKDEVMPLLAKLCFRPSDNSLGRAIKLTHYIELSEKYMKKLPDDWHIYVRNEADLPV
                     AKREHLMKLLEEKGWKIDWEKKKIIEGPLRKVDVSFQPTNVPRLCKEVKK"
     gene            4637..5206
                     /gene="cdhB"
                     /locus_tag="VKL21_07260"
     CDS             4637..5206
                     /gene="cdhB"
                     /locus_tag="VKL21_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase complex
                     subunit epsilon"
                     /protein_id="HMB45603.1"
                     /translation="MTPNTREKDIEKEEKIIDTTRQAVPFNTGNIPGPKMARAVMPAI
                     PGKMLAKAKRPLLMVGSEIDDPDVLERVVTLGKTGIQIASVGDSYKYLGDKGLDAHYV
                     NMHALMSYICDPEWPGLDGKGGYDLVVFFGITYYYASQAISALKNFSKIKVISIDKYY
                     HPNADMSFGNLKKDVFIEALDEVIEQLPR"
     gene            5246..6637
                     /gene="cdhC"
                     /locus_tag="VKL21_07265"
     CDS             5246..6637
                     /gene="cdhC"
                     /locus_tag="VKL21_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_function: GO:0043884 - CO-methylating acetyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HMB45604.1"
                     /translation="MANEFPFEISPMFEGERVRKDDMFVELAGPKSRGFELVRAAALD
                     EIEDGKFTLIGPDLSKMEEGSRHPYVMIYKIAGKLVEPDLEAIVERRNHDFQNYIQGY
                     MHLNQRYDVWVRINKDAIKKGLNSFEQIAKATMMLFKNELSFIEKIEATYITDPDEIE
                     KQRTEALKIYEARDARTKGLHDEDVDVFYGCTLCQNFAPTNACVVTPDRISLCGAINW
                     FDGRAAAKVDPEGPQFAIPKGEVIDAIGGEYSGVNEKAVALSGGEYSRIKIHSFFEYP
                     HTSCGCFEVVGFYIPEVDGIGWVDRDFTGIAPNGLAFSTMAGQTGGGKQIIGFLGVGV
                     NYFRSPKFIQPDGGWSRVVWMPKNLKEKIKADIPPELVDKLPTEENVTDLKTLKEYLE
                     KSQHPVMLRWTKEEAAAEAEAPSETAAPGWSMPSPSMPPGMPMQFDGGGTKLNFKNAK
                     ISIERIIFKSKEK"
     gene            6685..6921
                     /locus_tag="VKL21_07270"
     CDS             6685..6921
                     /locus_tag="VKL21_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HMB45605.1"
                     /translation="MEIIYTNHAEEKLAERKISKREIEKSLKNPDAVLDGRDDVKIVH
                     KLIRDKLLRIAYKSEKDFLLVITAYYTHPERYEE"
     gene            6924..>7116
                     /locus_tag="VKL21_07275"
     CDS             6924..>7116
                     /locus_tag="VKL21_07275"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HMB45606.1"
                     /translation="MKITYDPDADAMNIKFQSGKYNLSKEIAEGIVVDYTKDGKVLSI
                     EILDASKRMPMKSIKDITIG"
BASE COUNT         2227 a         1372 c         1605 g         1912 t
ORIGIN      
        1 tcctttgcat atctttgcag tgtttgattc ccgcacatct atggctattg ccattctgtc
       61 ttcctcatac ccgcgctggc gtgattctcc acgggggaaa tatgagaacc gtgaaagatg
      121 gcgaggctgg gagtcctgtg catccgaacc ccaataaaca agcacatcgg ctttattcct
      181 tatatcttcc agcgaacatg tccttaattt tttctgaagt attctttcca tgaggagccc
      241 ctggcaaaat gaagaagtat catctataac agcatttatt ttttttgcaa gctggatacc
      301 ttttgcctgt gcttcacatg ttgaattatc caggccaaaa agcatgggtg atctggcatt
      361 cttgataatc tcaacggttt tcctgatgga actttctatg tcggttggtt ttagatctat
      421 ggaaggaatt aacctgttcg tacaaccctt tattcttgca gcccctcgtc tgcatgcatt
      481 cctcgtttct tttatcctgt tattctcgac aattgcttca atgtcatcac ataaaagcgc
      541 gcatcctgtg catgtagcca taatccccct aaacaaattc tattgcccct gttgggcatg
      601 ggtctataca ggcataacac aataccctgc tttttccaaa gcgtcggcat tctttaatat
      661 tcgaagccat gacttttcca tcaacgactt tgaaaatcac cttctcattt gtaggagcgc
      721 accccatacc tgctccgtgc gggtcattcg ctacgtccac agggcatgca acaacacaat
      781 tcccgcatcc tgtgcataat tcaggatgta ctatcatacc tgtttcttct gctcccgccg
      841 atggcaaaac tagtgattcc aatttctctt tttgaagctt gaaattcacc tcaaggagtg
      901 gcgtgcattc ttctacgttc ttttttcttc caagcaaggc aaccgcaaat gccatacatg
      961 tggattctcc acatttcttg cagttggttt tgggtaagag ctggtatagc tccatgactg
     1021 taaccatagg tcgagaattg attctataaa gggatatatt ttatggcatt atgattttca
     1081 aaagtaaaaa atataaatag tatgaatcac taataataat attatgaaag tagttttcag
     1141 aattattggc tccgaggaag acctcaaaga cgttgaagga aaagaagaga atgtacattt
     1201 ctgtttcagg ccatctgaaa aaaacgtatt cgagctggta accaggacac caaaattaaa
     1261 aaggatacag ttaccatcat cttaccagaa aacaatttcg aacaccacaa aagcacttct
     1321 aaaaatgaaa aacataaaac ttctgaccgg ggatatctgg ggtcacagga cagatattga
     1381 cagatttgca gagatagaag tttgaattta ggatagaaag gtttaactca ttttcgggct
     1441 ttagtggcaa ctaaaaccat aagatgttca aaattaaatc tctgtttgtt cttttcttcg
     1501 atataaaacc caagtgactt catctttgat tttacttctt ctattccggt aagtgaggat
     1561 actagcatta agatttttcc atttttaagt aaattggaac cagcatcctc aagaaatctg
     1621 ttgataattt tcctgccatc tgatccgcca tccaatgcaa tattgatcca acctgttgtt
     1681 ctttcagaat cattagtagg aagatagggc ggattgaaaa ttatgatgtc aaatttcccc
     1741 tttatgcatt ttaataaatc gcctcttatt gcttctactc cattgtctct tgtgcattct
     1801 gctgcataag gattgatatc aatgcctgtg atctttgcat gtgtattctt cttaattaca
     1861 gcagagatta tcccgcttcc acacccaact tccaggatgt tttccgaatt attaataata
     1921 gacaaagctg cctctgtcag aagaaaggaa tcttcggcag gttcatagac ccaaggtgtg
     1981 aatttgatag ttgtattttt atagtgaata ttcataattt tctaataatg tgagttcttg
     2041 ggaattgctg attgtttttc atgggataaa actaatgccc taaaatattt ataatgtatg
     2101 ataatgataa aatgtgataa ctcttaaata tattgatgta aattagtgta taattagtca
     2161 ataaaagttg aatgattcaa atagcattgg taaattttat tatcctgtga ggtagtgagg
     2221 ataggtaaga aattcacccg gacatttgca agcagtggag ataataaatg accctgaaaa
     2281 aaggaagttt tgtaatagaa ggattggaga acgtccagat aaacatagga aaagcaggag
     2341 cagcagaaga acaaatagca gaggggccta cacccagacc tgagattata agtctgcgaa
     2401 actgggatta ccgcctcctt gacagatata atccagtata tactccgaca ggcgatacat
     2461 gtgattactg cacttatggt aaatgcgacc ttacaggaaa taaggaggga gcgtgcggaa
     2521 tagatcttga aggacagtcg gcacgccagg cgctaatgac aagtattatg ggtgctgcat
     2581 gccattctgc tcatgggagg caccttctca actatctcat aaagaagtac ggcgaagact
     2641 tcaaaattga cgttgggcca agtaacctgc aggcgcctct tactgaaaca attatgggga
     2701 tacagccaaa gacaattggt gattttatcc cggttcttga atatgttgaa gaacaactta
     2761 cacagcttat tgcggtcaca aatacaggcc aggaaggttc ttcccgtgac tttgaatcaa
     2821 aagctctgca tgcaggaatg ctcgacctgc tcggtatgga ggtcgctgat atcatccaga
     2881 tatcttgcct cggattccca aaatctgatg aaaaagcacc tatggccgag ataggtatgg
     2941 gaatacttga tcccaaaaaa cccgttgtta tatgcgttgg gcataacatt gcagcgccgg
     3001 catacattct tgatcacatg gataagaatg gattattcga taaaatagag attgcaggat
     3061 tgtgctgtac cgcccatgac atgacccgtt ataataaatc tgcaaagatc attggttcta
     3121 tgagcaagga acttaaatat atcaggtctg gaatacccga tgttctggtc acggacgaac
     3181 agtgcgtcag agcggatatt ttgaaaaaag cgcaaaaact tcacatccct gtgatcgcaa
     3241 cgaacgaaaa gatcacatac ggattaccgg acaggtcaaa tgacagcgtg gatgatatca
     3301 tagaagacct tgtgagtgga aaacagcctg gtgtagttct tcttgatttt gaaaagatcg
     3361 gggaacttgt ccctaaactt gcaatgaaga tgggaccaat aaggaaagct aaaggattaa
     3421 ctgcgctccc gaatgacaag gaattcaaga aacttgtcgc aaaatgtaca aaatgtctcc
     3481 agtgcacaaa ggattgccca cagggcctac caatttcgga tgctatgaca gccgcagtga
     3541 acggggattt ttcacttttt gagacactac atgataaatg cgttggatgc ggaagatgtg
     3601 attacagctg ccctgcgggc atcccgatat tgaatgttat cgagaaggct tcacagcggt
     3661 tgataagaga agaaaaaggt aaaatgagga ttggaagggg acaaatcggg gatcctgaga
     3721 tacgtgagga agggcgcaat cttgtccttg gaacaacacc tggggttctt gcttttgtag
     3781 gctgcggaaa ttatccggac ggtacaaagg atgtttatga tatcgttgaa gagatgatac
     3841 agcgcagtta tattataatt tcttcaggat gtgctgccat ggatataggg atgttcaaag
     3901 ataaagaggg aaagaccctt tatgaacgct atccaggaag atttgtaaag ggcaatctac
     3961 tcaatacggg ttcatgtgtt tcaaacgctc acatagctgc gacaactatt aaagtggctt
     4021 caatatttgc cggaagaaag acaaaaggaa attgggaaga gatcgctgat tatgtcatga
     4081 acagggttgg agcagttgga atagcatggg gagcttactc gcagaaagca tttgctatcg
     4141 gaacgggctg caacaggctt ggcattccgg ttgtggtcgg gccgcacggc acaaaataca
     4201 ggagggcgtt tatcgggaaa ccttataaga aagagaactg gaatgtactg gatggaagag
     4261 acggctcgat tgttaatgtt gaacctgcgc cggagcatct aatgataacc gcagaaacaa
     4321 aggatgaggt catgccactt cttgccaagc tctgcttcag gccaagcgat aacagccttg
     4381 ggcgggctat taaactgacc cattatattg aactcagtga aaaatacatg aaaaagctgc
     4441 cggatgactg gcatatttat gtgcgaaatg aagcggacct tccggtggca aagagggaac
     4501 atctcatgaa attgcttgaa gagaaagggt ggaagattga ctgggaaaag aagaagataa
     4561 ttgaaggacc gcttcgcaaa gttgatgttt ctttccagcc taccaatgtt ccacgccttt
     4621 gcaaggaggt caagaaatga cgcctaatac ccgggaaaaa gatattgaaa aagaagagaa
     4681 aatcatagat accacccgcc aggccgtacc atttaacacc ggaaatattc ccggtccgaa
     4741 aatggcgcgc gctgtgatgc ctgcaatccc gggaaaaatg cttgcaaagg caaaacgccc
     4801 tctactgatg gttggttctg aaattgatga tccggatgtt cttgaaagag ttgtcacgct
     4861 ggggaaaacc ggaatacaaa tcgcctcagt tggagactct tataaatacc tgggggataa
     4921 gggacttgat gcccattatg tgaatatgca tgcgctaatg tcatatattt gcgacccgga
     4981 gtggcctgga cttgacggaa aaggcggata cgaccttgtg gtctttttcg ggattaccta
     5041 ttattacgca tcccaggcaa tatcagctct taagaatttc tcaaagatca aagtaatatc
     5101 aatagataag tattatcatc ccaatgcaga tatgtcattc ggtaatctca agaaggatgt
     5161 gttcatcgaa gcgcttgatg aagtcataga acagttaccc aggtaaaata ataaatatga
     5221 aaattaatag aataggacga taatcatggc aaacgaattt ccctttgaaa tctcaccaat
     5281 gttcgagggt gagcgtgtac gaaaagacga tatgtttgtt gaacttgcag gaccaaaatc
     5341 acgtggattt gaacttgtaa gggcagcggc attagatgaa atagaggacg gaaaatttac
     5401 actaatcggg cctgacctct caaagatgga ggaaggttca cgtcatcctt atgtgatgat
     5461 atacaagatc gcaggtaaac tggtagaacc tgaccttgag gcgatagttg aaagacgaaa
     5521 ccatgatttc cagaattaca tccagggata catgcacctt aaccagcgat atgatgtctg
     5581 ggtgagaatt aataaagacg ccattaaaaa gggattaaat tcgtttgaac agatagctaa
     5641 agccacaatg atgcttttta agaacgaatt gtcctttata gagaaaatcg aagcgaccta
     5701 tataactgat ccggatgaga ttgaaaaaca aagaacagaa gccctaaaga tctatgaagc
     5761 aagggatgca agaactaagg ggctacacga tgaggatgtg gatgtgttct atgggtgcac
     5821 attatgccag aactttgccc ccacaaatgc atgtgtcgtt acgcctgaca ggatatccct
     5881 ttgcggggct atcaactggt ttgacggccg cgctgccgcc aaggttgacc cggaaggacc
     5941 gcagttcgcc attccaaaag gtgaagttat cgacgccatc ggaggagaat attcaggcgt
     6001 caatgagaag gctgtagcgc tatccggtgg tgaatattca cgcattaaga tacattcatt
     6061 ttttgaatac ccccatacaa gctgcgggtg ctttgaggtt gtaggcttct atattcccga
     6121 agtagatggg atcggctggg tggacaggga tttcacaggt attgccccga atggtcttgc
     6181 tttctccaca atggcaggac aaaccggagg gggtaagcag atcataggat tcctcggggt
     6241 tggtgtgaat tatttccgct ccccaaaatt catacagcct gatggcggat ggagccgtgt
     6301 tgtgtggatg ccaaagaacc tcaaggaaaa aataaaggcg gatattcctc ccgaacttgt
     6361 ggataaattg cccactgaag agaatgtcac tgaccttaag accctgaaag aatatcttga
     6421 gaagagccag catccggtaa tgctgcgctg gacaaaggaa gaagctgccg ccgaagcaga
     6481 agcgccatca gagacggcag cgccagggtg gtcaatgcca tcgccttcca tgccaccagg
     6541 gatgcccatg caattcgatg gtggtggaac gaagctcaat ttcaaaaatg caaagatttc
     6601 tattgagcgc ataatattta agagcaaaga gaaataaggc gcaggtaaat cttctttgcg
     6661 tactttgtgg ttattaaata attcatggaa attatttata caaatcatgc agaagaaaaa
     6721 ttagctgaaa ggaaaatctc aaaaagggag attgaaaaat cacttaagaa tcctgatgct
     6781 gttctggatg gtagagatga tgttaaaatc gtgcataaac ttattagaga taagctctta
     6841 agaattgcat ataaatccga aaaagatttt ttgctagtaa taaccgctta ttacactcac
     6901 ccagaaagat acgaggaata ataatgaaaa taacatatga tcctgatgca gatgctatga
     6961 atataaaatt tcagtctgga aaatataacc ttagcaaaga gattgcagaa ggaattgttg
     7021 tagattatac taaagatggt aaagttcttt ccatagaaat cctggatgca tcgaaaagaa
     7081 tgcctatgaa aagtattaaa gatattacca ttggca
//
LOCUS       DATGBN010000300        11420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_450870, whole genome shotgun sequence.
ACCESSION   DATGBN010000300 DATGBN010000000
VERSION     DATGBN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450870"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VKL21_07280"
     CDS             complement(<1..600)
                     /locus_tag="VKL21_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein"
                     /protein_id="HMB45607.1"
                     /translation="MENISKSADLIFLKIKKLPFILLGERIKARKEKFRDMRMSLRQA
                     RIPISYEMYVSNAVFYSVIAGIFGAIMGLIIAYVIVSIVGLPDRITHITFSESTAWLL
                     RYKTISVSILIVLFLGGLFGIITYMLFLVYPGFRAGERKGDIDKNLPYAVTYMYALSR
                     GGMNVIEIFRSLSRCASTYGEVSKEMDVIIRDMDYFGNDL"
     gene            complement(606..2783)
                     /locus_tag="VKL21_07285"
     CDS             complement(606..2783)
                     /locus_tag="VKL21_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HMB45608.1"
                     /translation="MVQIEKKLEGKVSGRPSAALFESKKGKKRKGIKKGRVFSEEITS
                     EKETKPSIEISSNIDNPVEKPIEKAIEEGENNKIIFLPEKTESETTTSEEETKPPAEI
                     RSNIDNPIEKSAFEEHENEINTIPEQKEGKSTPEEKRPLEGMDSIMKRLEVDKTRDLI
                     KEQMTPEKQAVYDIMEDKLVSDIETRLKKLKEIQSPDKSEKPKTFVERIKLLLTKEEG
                     EKESYDPSTHGPLVTFEGIKGYNELERYWVNEPYALVVILFNPEKNTNVYYVVEPKLS
                     DFDDLFLREIKDRLKDVLLFEDIKNDEDKDKILTDKVKRLVKEYSIDISPLTTEKILY
                     FTRRDFIKFGKIDPLMHDDRIEDVSANGFGVPIYLYHKKYTNLATNVIYREDELDSYV
                     IRLAQRCGKHISVAEPMIDATMPDGSRIQMTLGTTITTHGATFTIRKFSEVPITPVDL
                     INWNTFSAEEMAYLWICIENNKSLIYAGGTASGKTSSLNAVSLFIPWQAKIISLEDTR
                     ELKLPHPNWIPGVTRDSFTADGKGTIDMYDLLKAALRQRPEFLLVGEVRGKEALTLFQ
                     AMSTGHTTFSTMHADSVSSAIHRLENPPISVPRTMIQALNIISIQSQTYVKGKRARRN
                     MKLVEITDIDPTTRNLRTNDIFAWDALTDKFNRVGESKALNEITIRRGWNVGDMKKEL
                     MNRQKILEFMVNNKISDFNAIATIIHDYQVTPEKVLKKLLISG"
     gene            complement(2846..3043)
                     /locus_tag="VKL21_07290"
     CDS             complement(2846..3043)
                     /locus_tag="VKL21_07290"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45609.1"
                     /translation="MENLMFLTLILLAVIFFAFIFIYLMYVRMKQLETELHDLKSRIS
                     ITDEELTRLSKDIEDFKKINI"
     gene            complement(3045..3833)
                     /gene="minD"
                     /locus_tag="VKL21_07295"
     CDS             complement(3045..3833)
                     /gene="minD"
                     /locus_tag="VKL21_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HMB45610.1"
                     /translation="MTAHVYTIASGKGGTGKTTTTLNLGTALALMGKKTVVLDADIGM
                     ANLGLLIGLERCKITLHEVMSGSANIKEAIYDGPGGLKVVPSGLSLKGFQKSNPDILK
                     DVMSTLVEGMDFMLIDAPAGISRDGVIPLAVADQVILVVNPELASMADALKTKALTEM
                     LGRSVEGAILNRATLEKTEINRNKVSELLGVKILEMIPEDPNVRLSAAFKTPIVVKSP
                     NSPASIAYKRLAAQLAGEKFVETSVEKNKDGFVDRLVKSIFGGK"
     gene            complement(4094..5053)
                     /gene="dusB"
                     /locus_tag="VKL21_07300"
     CDS             complement(4094..5053)
                     /gene="dusB"
                     /locus_tag="VKL21_07300"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMB45611.1"
                     /translation="MNSLVIGKLRLDGNLILAPMSGVTNLPSRLLCRKYGASLVYSEM
                     TSSEAVVRQNLRSIERGFTCQGERPMGIQLMGSLPANLAASALFLQERYSPELIDVNF
                     GCPAQSVIKNGCGSELLKKPELIGQIVKSLSDSLDIPVTAKIRILGDIDETLGIARII
                     ENSGADAITVHGRTQKQGYSGKCNLDFIKKIKSELSIPVIANGDVFDEKTAKQIIEYT
                     QCDGLMIGRAAIGNPFIFRKISHFLKTGEILPPQEVEERLADFFEYMELCRNYGMLTY
                     NDMKRNAQWFTKGMENVKQVRVKINKTEDIDSIIEILNDILAS"
     gene            complement(5153..5326)
                     /locus_tag="VKL21_07305"
     CDS             complement(5153..5326)
                     /locus_tag="VKL21_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45612.1"
                     /translation="MVKNEKKKVFCSSIEFKKTYFPKSFEKQAVKELEDAHNLGIRWA
                     KESLDKIKCQIQR"
     gene            complement(5313..6440)
                     /locus_tag="VKL21_07310"
     CDS             complement(5313..6440)
                     /locus_tag="VKL21_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45613.1"
                     /translation="MGNNNQIYRAKLGKKKFGTLKEYSDDFIAFLDNGNPLFPDSVQE
                     SYVMTSIYGYFYFIKNEIEKEVESVISRKNEITQGDVKQITSNIIIKHYENWKNADLL
                     PSIPNSHTENIINKYNKNIEEAIFEIFEKLPLYKRHSDQLKEIAGTLFSKSYIIPDTV
                     SGVVIAGFGEIDVFPSLRRYVIEGVANNRLKYTEIPPVEINFETHASIIPIAQREMVD
                     TFMMGVDPSYMELEESYITEIFSEYTDIITNNINKYDTKEKLKLKKKLLKVSNEILKD
                     HQEKLRIYRKETYTNPVTSVVSMLPKDELAAMAEALVNLTSFKRKVSMETETVGGPID
                     VAVISKGDGFIWIKRKHYFKPELNPGFFANYYKEEIEDGEK"
     gene            complement(6404..6622)
                     /locus_tag="VKL21_07315"
     CDS             complement(6404..6622)
                     /locus_tag="VKL21_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45614.1"
                     /translation="MTTEIAIMNKHAIALASDSAVTMSQEAGEKIKTSANKLFALSRY
                     HPVGIMIYGSADIMEVPWETIIKYIEQN"
     gene            complement(6821..8242)
                     /locus_tag="VKL21_07320"
     CDS             complement(6821..8242)
                     /locus_tag="VKL21_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMB45615.1"
                     /translation="MVDTILSCTTCEMCDLRCSASLPIEPSWMKLRGKLINEEKKMTF
                     PPFEMMTEALKSQGNIWAGYRTDRSKWFPEELKEKHGPSHKSKNVYFAGCTASFVEKD
                     IGIASVRLLDEAGLDFTYLGEKENCCAPPVLVAGKWDVFEQTMKKNISAVKAAGADTV
                     ITSCPACDMMWRKVYPEWADKLGIDFKIETKHYSEIVSEKIKSGEFKFKKGLEKPVTV
                     TWHDSCHIGRASGIYEPPRDLIKAIPGVTLVEMSSNREKAHCCGSVLTLLKEPDVAAD
                     IGKIRLDEAVEAGAQKVLALCPCCQFQLRVSADARNIPIEVVDLASFASSAMGYDLPD
                     PNPEVQAQWAVFEKMITLMTPRGFADIMKTMWPELIDAMPYGMGPMMRAMGKIPGALT
                     RMKPMFPVLFPILLPKMMPKVMSVILEKVGQAIPMPDYMAQKMPELMPKVMDNLMPHM
                     IDDVVPLITQPMIDYLQGKGTCQ"
     gene            complement(8155..8766)
                     /locus_tag="VKL21_07325"
     CDS             complement(8155..8766)
                     /locus_tag="VKL21_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45616.1"
                     /translation="MEDILGFIPSDQRKFSYNFVFGLALSIIRVAEKHGGRPYATGLF
                     FSKKADSVLGKERVKRLRAFKNKVDHDNIMNPGKVIGSGPVGIALQIAGTLEPFIRPL
                     GNSVTTLVGERPEKEIRGIPKDVAWYAYSCSQCGYCVDECDQSTGAAGKARVQEGNGT
                     GFGSIWKAACNGTRRWSIRSFPVRPARCAICAVRHHCQLNLRG"
     gene            complement(8714..9487)
                     /locus_tag="VKL21_07330"
     CDS             complement(8714..9487)
                     /locus_tag="VKL21_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMB45617.1"
                     /translation="MCYIDKTVDNYELVRPLIGNTTPDAVVQPQDEEELIEIVRWAIL
                     NKVPLTPRGKATSGYGGAIPVNKGIVIDFYRMKKVLAIDPKNKTVRVQPGITWEKLDT
                     ELNKQGLTLRLYPTSYPSSTVGGWLAQGGAGIGSYEYGYFRENVESAKVVLPSGDVKV
                     FSGNELELIADAEGITGLIIEVKLRIQPREDIEVAALACSDANRLQELVNSIIESKLP
                     IWSMIFINPKMAEMKNRAPLMEHGGHSGFHSERPEKIQL"
     gene            9637..10047
                     /locus_tag="VKL21_07335"
     CDS             9637..10047
                     /locus_tag="VKL21_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45618.1"
                     /translation="MKDGNMALDIKTLATINLMIQIVLMIVLFFAAFRVKKGEVNNHC
                     NYIKIAVLLQLAAIFIFMLPSFPGYRISNGSLLIIFHHALGSVLVGLWIYVNLVYSRY
                     IRWPENFRYIMRIAFVLWILAFLIGSYIYIRIYY"
     gene            10097..11239
                     /locus_tag="VKL21_07340"
     CDS             10097..11239
                     /locus_tag="VKL21_07340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMB45619.1"
                     /translation="MISEKAAKLPPFYVMEVLERAKELESMGKDIIHLEIGEPDFPTA
                     PHICEAASFALTKGETKYTHSQGLLELREAIARHYYVKFKLDPDPARILVTSGTSPAL
                     LLVFMSLIEHGDEVIMSDPYYACYPNFVRYLGGHPVFVRTKEENGFSMGFDTIARAIT
                     SRTKAILINSPCNPTGHVMSPENLRGISEIAGNIPVISDEIYQGLIYQGKDHSILEYT
                     SNAFVLNGFSKLYAMTGWRLGYLIAPKEYIRTLRKIQQNFFICANSFVQHSGIAALTG
                     PQDQVREMVATYDTRRKFIIKRLRDIGFGIKYEPCGAYYVLANAKKFSPDSLDLSRRI
                     LEEAGVAVTPGIDFGDGAKGYLRFSYANSIENIKEGMDRIEKYLHT"
BASE COUNT         3084 a         2515 c         2103 g         3718 t
ORIGIN      
        1 taagtcattt ccgaaataat ccatatcccg gattatgaca tccatttcct ttgatacctc
       61 gccatatgtg ctggcgcatc tgctcagcga gcgaaatatt tcaattacgt tcatccctcc
      121 cctgctcaat gcatacatat atgtaacggc gtaaggcagg tttttgtcaa tatctccctt
      181 tctttctccg gccctgaaac ccggatacac aagaaagagc atataggtaa tgatcccgaa
      241 caatccccca aggaaaagaa cgatcaatat cgatactgat attgtcttat aacgcagcaa
      301 ccatgcagtt gactcagaga aagtaatatg agtgatcctg tccgggagac ccactatgga
      361 tacaatgaca tatgcaatta ttaaacccat gatggcccca aaaatacccg ctattaccga
      421 ataaaaaacc gcgtttgata catacatttc atacgagatg ggaattcgcg cctgccttaa
      481 tgacattcgc atatcacgga acttttcttt tcgcgctttt atcctttcac caagtaagat
      541 aaatggcagt ttcttaattt ttaagaatat caaatccgca gatttactaa tattttccat
      601 tttcttcatc cggatatcaa taatttcttg aggacttttt ctggagtgac ctggtaatca
      661 tgaatgatcg tagctatcgc attaaaatca gatattttgt tattcaccat aaactccaga
      721 attttttgcc ggttcattaa ttcttttttc atatccccaa cattccatcc ccttctgatg
      781 gttatttcgt tgagcgcttt tgattctccg accctgttga atttatctgt cagggcatcc
      841 catgcaaaaa tatcattcgt cctgaggttc ctcgttgttg gatcaatatc agttatctct
      901 acaagtttca tattgcgtct tgcccttttt ccttttacat aggtttgcga ctggatactg
      961 atgatattaa gcgcctgtat catggtcctg ggaacgctga taggcggatt ctcaagtcga
     1021 tggatagcgc ttgaaaccga atcggcatgc attgtggaaa aagtcgtatg acctgtgctc
     1081 attgcctgga aaagtgtaag cgcttccttg cccctgacct cacccaccag aaggaattcc
     1141 ggcctctgcc gaagtgcggc ttttaacagg tcgtacatat caatagtccc ttttccatca
     1201 gcagtaaaag agtccctggt tacacccggg atccagttag gatgcggaag ttttaattcc
     1261 ctcgtatctt caaggctgat tattttagcc tgccagggaa taaacaatga aaccgcatta
     1321 agagaagatg tctttccgga cgctgtaccg cccgcataaa ttaaactctt attgttttcg
     1381 atgcaaatcc atagatatgc catttcttcg gctgagaagg tattccaatt tatcagatca
     1441 acaggcgtta tcgggacttc gctgaacttt cttatagtaa aagtagcgcc atgtgttgtt
     1501 attgttgtcc ctagtgtcat ctggatgcgt gatccgtccg gcatagtagc gtcaatcatg
     1561 ggttcggcga ctgaaatatg ctttccgcat cgctgggcaa gccttataac ataagaatca
     1621 agttcatctt ccctataaat tacgtttgtt gccagatttg tatatttctt atgatataag
     1681 tatataggaa caccaaaacc attcgctgaa acatcttcta tcctgtcatc atgcatgagg
     1741 ggatcgattt tcccgaattt tataaaatcc ctgcgcgtaa aatataatat tttttctgta
     1801 gttaaagggc ttatgtctat tgaatattct ttaaccaatc ttttgacttt atcagtaaga
     1861 atcttgtctt tatcttcgtc atttttaata tcttcaaaaa gaagtacatc ttttagcctg
     1921 tctttgattt cccggaggaa taaatcatca aaatcagaaa gttttggttc aaccacataa
     1981 taaacatttg tattcttttc aggattgaag agaattacca ccagagcgta tggctcatta
     2041 acccagtagc gttccagttc attatatcct ttaatacctt cgaacgtaac aagtggtccg
     2101 tgagtagaag ggtcatatga ttccttttct ccttcttcct ttgtgagtaa tagtttgatt
     2161 ctttccacga aggttttggg tttttcactt ttatccggag attgtatttc tttaagcttc
     2221 tttaaacggg tctcaatatc tgaaactaat ttatcttcca tgatatcgta gacagcctgc
     2281 ttttccgggg tcatctgctc ttttatgagg tctcttgtct tatcaacctc aaggcgcttc
     2341 ataatgctgt ccatcccttc aagagggcgt ttttcttccg gggttgattt tccctccttt
     2401 tgttcaggta tcgtatttat ttcattctca tgttcttcaa aagctgattt ttcaatcgga
     2461 ttatcaatat ttgatctgat ttctgcagga ggttttgttt cttcttctga agttgtcgtt
     2521 tctgattcag ttttttccgg taaaaaaata attttgttat tctctccctc ctctatcgcc
     2581 ttttcaatcg gtttttcaac cggattatca atatttgaac tgatctctat ggaaggtttt
     2641 gtttcttttt ccgaagttat ctcttcggaa aatacccttc cttttttgat tccctttcgc
     2701 ttttttccct tttttgattc aaatagcgct gcgcttggcc gcccactcac tttgccttcc
     2761 aacttttttt ctatctgaac cattttttaa acgtccctcc aaattgtctc agcttaagat
     2821 ttttttttta ataatctatt taatattata tatttatctt cttgaaatcc tcaatatctt
     2881 ttgataagcg ggtaagctct tcatctgtaa tagaaatcct gctcttgagg tcatggagtt
     2941 ctgtttccaa ttgtttcatc cttacataca tcaaataaat gaatataaat gcaaaaaata
     3001 ttaccgccaa tagaattaat gtcagaaaca ttagattttc cattctattt ccctccaaaa
     3061 atggacttaa ccagacgatc tacaaaaccg tccttatttt tttccacgct tgtttccacg
     3121 aatttctcgc ccgcaagttg cgcagcaagc cgtttgtatg cgatagaagc cggtgaattc
     3181 ggggacttca caactatagg cgttttgaat gccgcagaaa gtcttacatt tggatcttca
     3241 gggatcattt caagtatctt tacacccaga agctcagata ctttatttcg gttaatttcc
     3301 gttttctcaa gcgttgcccg gtttaaaata gcgccttcaa cgctccttcc cagcatttcg
     3361 gtaagtgctt tggttttcaa tgcatccgcc attgaggcaa gttcgggatt cacaacaaga
     3421 ataacctgat cggcaacagc aagtggaata accccgtccc tgctaatgcc agcgggagca
     3481 tctattaaca taaaatccat tccttcaaca agagtggaca ttacatcttt taatatatct
     3541 ggatttgatt tctggaatcc cttcaatgat aagccgctgg gcactacttt aagacccccc
     3601 ggaccatcat aaatagcctc tttaatatta gcgctaccac tcatcacttc atgcagcgtt
     3661 attttgcatc tttcaaggcc gatcaaaaga cccaggttcg ccatcccgat atccgcatcg
     3721 agaacaactg tcttcttgcc cataagtgcc agtgcggttc caagattgag agtagtcgtg
     3781 gtcttgcccg taccaccttt tcctgacgcg attgtatata cgtgtgctgt catgctgatt
     3841 ccctcgacaa tgatacgtcg atgattctta tgatatgtat cattatactc ataaggggtt
     3901 aataaaccta acctatattt tattctgact tcgccatttt aatggatttt ttgaggattt
     3961 tgaagaaata taataaaaaa taaatctaat atagataatc aaccacggag gacacataga
     4021 tcacagagtt gtaattctct atgttctctg cggactctgt ggtttcttag caggaagcta
     4081 ctactgtttt ttttcacgat gcaagaatgt cgttcaatat ctcaataata gaatcaatat
     4141 cctccgtctt atttattttc acacgaacct gtttcacatt ttccatacct tttgtaaacc
     4201 actgggcatt tcgcttcata tcattatatg taagcatacc ataattgcgg catagttcca
     4261 tgtactcaaa aaaatcggct aatctttctt ccacctcctg gggaggcaat atttccccgg
     4321 tttttagaaa atgggagatc ttcctgaaaa taaatggatt tccgatagct gcacgtccta
     4381 tcataagtcc gtcgcattgt gtgtattcga taatttgttt tgcggttttt tcatcaaaga
     4441 catccccatt cgctataact ggaatcgaaa gctcactttt tattttttta atgaaatcaa
     4501 gattacattt ccccgaataa ccctgttttt gcgttcttcc atggactgtg atcgcatctg
     4561 cccctgaatt ttcaataatc ctggcgattc ctaatgtttc atcaatgtcc cctaatattc
     4621 ttattttggc tgttacgggt atgtccaatg aatccgaaag cgatttcact atctgtccaa
     4681 tcagttcggg tttttttaaa agttctgatc cgcatccgtt ttttattaca ctctgcgcag
     4741 gacagccaaa atttacatct ataagttcgg gactgtatct ctcctgcagg aaaagtgcag
     4801 atgctgcaag attggcaggg agagaaccca ttaattggat ccccatgggt ctttcaccct
     4861 ggcatgtaaa tccccgttct atacttctca ggttttgccg aactactgcc tcggaagagg
     4921 tcatctctga ataaacaaga gatgcgccat atttcctgca caggagcctg gatggaagat
     4981 ttgtaacacc ggacatggga gcaagaataa gatttccatc caatctcaat ttaccaataa
     5041 ccagagagtt catcgtaaga catgcctttt tacgctcgag gtttttcagt tcaattaatt
     5101 tttagggaat ctgcttcctt ttcaagcgat tgttatatat ttggtatgga tattatcttt
     5161 gtatttgaca ttttatttta tctagtgatt cttttgccca acggattcct aaattatgag
     5221 catcttctaa ttctttcact gcttgtttct caaatgactt agggaaataa gttttcttga
     5281 actcgatact agagcagaat acctttttct tttcattttt caccatcttc aatttcctcc
     5341 ttataataat tagcaaaaaa ccccggattt agttctggtt taaaataatg ttttctttta
     5401 atccatataa aaccatcgcc ttttgaaatt acggcaacat cgatgggccc tccgacagtt
     5461 tcagtttcca ttgaaacttt tcgcttaaag gatgttaagt ttacaagcgc ttctgccatt
     5521 gccgcaagct catcttttgg aagcattgat acaacactag taactggatt agtatatgtt
     5581 tcttttctgt atattcttaa tttttcttga tgatctttaa gaatttcgtt ggatactttt
     5641 agtaattttt tcttaagttt tagtttctct ttggtgtcat atttatttat attattagta
     5701 attatatccg tatactcgga gaatatttca gtgatataac tttcttcaag ttccatgtaa
     5761 gatggatcta cccccatcat gaaagtgtca accatttctc tttgcgcaat gggtataatt
     5821 gatgcatgtg tttcaaagtt aatttcaaca ggtggaattt ctgtgtattt taatctatta
     5881 ttagctactc cttcaataac atatcttcta agagaaggaa atacatctat ctctccgaag
     5941 ccagcaataa cgactccaga aacagtatca ggaattatat aagatttgga gaacaaagtt
     6001 ccagcaattt cttttaattg atctgaatga cgcttgtaga gtggcaattt ttcaaatatt
     6061 tcaaatatcg cctcttcgat atttttatta tatttattta ttatgttttc agtatggctg
     6121 tttggtattg aaggaagtaa atccgcattt ttccaattct cgtagtgctt tataataata
     6181 tttgaagtaa tctgttttac atcgccttga gtaatctcgt tttttctaga aataacagat
     6241 tctacctctt tttcaatctc gtttttaatg aaatagaaat atccatagat tgaagtcatt
     6301 acataacttt cttgcactga atcaggaaat agaggattac cattatccaa aaatgctata
     6361 aaatcatccg aatattcttt taaagttcca aacttcttct tacctaattt tgctctatat
     6421 atttgattat tgtttcccaa ggaacttcca tgatatcagc actgccgtat atcattattc
     6481 caacaggatg gtatctagat aaagcaaaga gtttatttgc cgatgtctta attttttccc
     6541 ccgcttcttg cgacattgta actgcactat ctgatgcaag agctatggca tgtttattca
     6601 ttattgcaat ttcagttgtc atttctatac tcttatacta ttactattgt tattaatcta
     6661 atgattctca catatctatg gtactcagat cccccaaaat acctctctac catatcccat
     6721 ccattcccga gagaaacatg aaagaagcta aggcacgcat gtatggacgc cgcatgtcgt
     6781 ggtgcttatg ataacttttt gaatcccact aataaactct ttattggcag gtcccttttc
     6841 cctgcagata atcgatcata ggctgtgtaa taaggggcac aacatcatcg atcatatgag
     6901 gcatcaggtt atccatgacc tttggcatca gttcaggcat cttctgcgcc atataatcag
     6961 gcatcggaat agcctggcct accttttcaa ggatcacaga cattaccttt ggcatcatct
     7021 ttggaagaag tatcgggaaa agcaccggga acatcggctt catacgagtc agagcgcccg
     7081 ggatcttgcc catagcacgc atcatcggcc ccattccata tggcattgca tcgataagtt
     7141 caggccacat ggttttcata atgtctgcaa atcccctggg cgtcataagg gttatcatct
     7201 tctcaaaaac agcccactgc gcctgaactt caggattcgg atccggcagg tcatacccca
     7261 tagcggatga agcaaagctt gcaaggtcaa ctacctctat tgggatattt cttgcatctg
     7321 cgcttaccct taactggaac tggcagcatg gacaaagagc aaggactttt tgggcgcctg
     7381 cctcaacggc ttcatccagg cgaatttttc caatgtctgc cgcaacatcc ggctctttca
     7441 ggagcgtaag tacggagccg cagcaatggg ctttctccct gttcgagctc atctcgacga
     7501 gagtgacgcc cggaatagcc ttgataagat cgcgcggcgg ttcatatatg ccggaggctc
     7561 gccctatgtg gcatgagtca tgccatgtta cggtgacggg tttttcaagg ccctttttga
     7621 atttgaattc accggatttg atcttctcag aaacgatttc actataatgt tttgtttcta
     7681 tcttaaaatc aattccaagt ttgtcagccc attcaggata aaccttgcgc cacatcatgt
     7741 cacatgctgg acatgatgtt ataacagtat cagcgcctgc tgcttttact gcgctgatat
     7801 tctttttcat tgtctgctca aagacatccc atttgccagc cacaagcacc ggaggggcgc
     7861 agcaattctc tttttctccg agatatgtga aatcaagacc tgcttcatca agaagacgca
     7921 cgcttgcgat ccctatgtct ttttcaacaa agcttgccgt gcagcctgca aaataaacat
     7981 tctttgactt atgggatggg ccgtgttttt ccttcaactc ttcagggaac cattttgacc
     8041 ggtctgttct gtaacccgcc catatatttc cctgggattt gagcgcttct gtcatcattt
     8101 caaatggcgg gaatgtcatt ttcttttctt catttatcag tttaccgcgc agtttcatcc
     8161 acgaaggttc aattggcaat gatgccgaac agcgcagatc gcacatctcg caggtcgtac
     8221 aggaaaggat cgtatcgacc atcttctggt cccattgcac gcggccttcc atatactccc
     8281 gaagccagta ccatttccct cttggactct ggctttccca gccgcgcccg tagactggtc
     8341 gcactcatcc acacaatatc cgcactgcga gcagctgtaa gcgtaccatg ccacatcttt
     8401 tgggatgccc cggatctctt tttcaggtct ttccccgaca agagtggtga ccgagttgcc
     8461 aaggggacgg atgaagggct ctaaagttcc ggctatttgc agggcgatcc ccacgggccc
     8521 gcttccaatg acctttcccg ggttcattat attatcatga tcaactttat tcttaaaagc
     8581 gcgcaatcgc ttaactcgtt cttttcctaa tacggaatca gcttttttcg agaaaaacag
     8641 cccggtagca tagggtcttc cgccatgttt ttctgcgacc cttattattg aaagcgcaag
     8701 accaaagacg aaattatagc tgaattttct ctggtcgctc ggaatgaaac ccagaatgtc
     8761 ctccatgttc cataagcgga gcgcgatttt tcatttcagc catctttgga tttataaata
     8821 tcattgacca gatcggcagt tttgattcaa tgatagaatt cacaagttcc tggaggcgat
     8881 tggcatctga acatgcaaga gctgccactt caatatcttc acgcggctgt attcgaagtt
     8941 taacttctat aattaatccg gttatgcctt cagcatcagc tattaactca agctcattac
     9001 cggaaaaaac ctttacatct cctgacggga gaactacttt tgcggactct acattttcac
     9061 ggaaataccc atactcataa cttcctatgc ctgcgcctcc ctgggccagc catcctccaa
     9121 cagttgagga gggatagctt gtaggatata atcgcagggt caaaccctgt ttatttagtt
     9181 ccgtatcaag cttttcccat gtgatgcccg gctggactct gacagttttg ttcttcggat
     9241 cgatagccag cacttttttc atcctgtaga aatcaatgac aataccctta ttgacaggta
     9301 tcgctccccc ataaccggaa gtagctttgc ctctcggggt gagtgggact ttgtttaata
     9361 ttgcccacct cacaatttca atgagttctt cttcatcctg tggctgaact accgcatcag
     9421 gagtggtatt tccgatcagg ggcctgacaa gttcataatt atccacagtt ttgtcaatat
     9481 aacacataac ttactacaat ataaagttat gtgcgtttat tccacaacca tacaaatggc
     9541 aagattctag aatttacgta aatatttact aatgaaaaaa acacaggttt gcctcaggct
     9601 catcaattta tatcgtctta agacatcata agaaaattga aggatggaaa tatggcgctg
     9661 gatataaaaa cactggctac gataaatctc atgatccaaa tcgtgcttat gatcgtgctt
     9721 ttttttgctg ctttcagggt aaaaaaggga gaagtaaata atcactgtaa ttatatcaaa
     9781 attgcagttc ttcttcaact tgcagctatt ttcattttca tgctgccttc atttcccgga
     9841 tacagaataa gtaatggcag ccttctgata attttccacc atgctctggg atcggtattg
     9901 gtgggattgt ggatatatgt gaatcttgta tattccagat atatcaggtg gcccgaaaat
     9961 ttcagatata tcatgaggat cgcatttgtt ctctggatac tggctttctt gattggatcg
    10021 tatatctaca tacggatata ttattgatat atttgacccg catgttataa gaatatcaaa
    10081 aacatcatct gtaaatatga tctcagaaaa agcggcaaag cttccccctt tttatgtgat
    10141 ggaagtactg gaaagggcca aggagcttga aagtatggga aaagatatca tccatcttga
    10201 aatcggggaa cccgattttc ctacagcgcc gcatatatgt gaagcagcgt cctttgctct
    10261 tacaaaaggc gaaacaaaat atacccacag ccagggactt ttggaactca gggaggccat
    10321 agcccggcat tattacgtga aattcaaact tgatccggat cctgcccgga tacttgtaac
    10381 ctcaggaaca agtccggcgc tccttctggt ttttatgtct ctcatcgaac acggtgatga
    10441 ggtcataatg tctgatccat attatgcatg ttatcctaat tttgtcagat atctcggagg
    10501 ccatccggtt tttgtacgca caaaagaaga aaatggattc agtatgggat ttgatacgat
    10561 tgctagagcc atcacctcaa gaacaaaagc catccttata aattccccat gcaatcccac
    10621 aggccatgtg atgtcgcctg agaatttgag agggatttct gagattgccg ggaatatccc
    10681 tgtgatctcg gatgaaattt accagggact tatctatcaa ggaaaagacc attccatttt
    10741 agaatatact tccaacgcct tcgtattgaa cggcttttcc aaactgtatg cgatgacagg
    10801 atggcgtctt ggttatctga tcgctccgaa agaatatata cgcacactcc ggaagatcca
    10861 gcagaatttc tttatatgcg ccaacagttt tgtccagcat tcaggcattg cagcgttgac
    10921 aggcccccag gatcaggtca gggaaatggt ggccacatat gatacccggc gcaaattcat
    10981 tatcaagagg ctaagagata tcggattcgg gataaaatat gaaccttgtg gcgcttatta
    11041 cgttcttgcc aatgcgaaaa aattcagccc ggattccctg gacttgagcc gcaggatcct
    11101 tgaagaagca ggcgtagcag taacacccgg gatcgatttc ggggatgggg caaaaggtta
    11161 tcttcgtttt tcttatgcga atagtattga gaatatcaag gaaggaatgg acaggattga
    11221 gaaatatctt catacgtgag ctataaatta aaagaaaaat aaacatatag tggtcatatg
    11281 acaaaaaaag aattggatcc ttatgtaact ttgatctgca cccattgtgc attcttcaag
    11341 gaggatgatc tggacctgga atgtgctgca tttaaggtct taaaatccat gcttcaaaaa
    11401 gataagatca cacaggaaga
//
LOCUS       DATGBN010000301         8083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_528443, whole genome shotgun sequence.
ACCESSION   DATGBN010000301 DATGBN010000000
VERSION     DATGBN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528443"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VKL21_07345"
     CDS             <1..418
                     /locus_tag="VKL21_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45620.1"
                     /translation="YFYKFVRVNKRKFFGFEKIKISDAEINIADKEKAIIDCLDREEY
                     AGSVIEILKALHNNKEQFDFDKMINYALIMENHSLARRLGFLLDLVKQDTKKLEERIG
                     KFRYVYLSNTLPKKKHGISKKWRLILNLEENELFKW"
     gene            458..631
                     /locus_tag="VKL21_07350"
     CDS             458..631
                     /locus_tag="VKL21_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45621.1"
                     /translation="MHKLYLHKRLSVDLDFTALDEFDIDIFKNILLIKEINSQIRKIR
                     AFENSVSIDLKYI"
     gene            659..877
                     /locus_tag="VKL21_07355"
     CDS             659..877
                     /locus_tag="VKL21_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45622.1"
                     /translation="MWLFLKELEFKDFEKLAEKKLAAMNDNMDIGKVFADLDVVKSLW
                     KVDLEQLIPELPDFDGVMNDLKKKLDLH"
     gene            complement(897..1319)
                     /gene="mutT"
                     /locus_tag="VKL21_07360"
     CDS             complement(897..1319)
                     /gene="mutT"
                     /locus_tag="VKL21_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HMB45623.1"
                     /translation="MKKVTAAIIIKDGLFFIAKRKKADTLANKWEFPGGKIENGETAE
                     ECLKREIEEELKINVRIGDFFGESIYQYPQGAIQLLAYWVFWESGSIELMAHDEYKWV
                     PLTELDYYDFAPADIHLVKKLKDGERVVTICQTPSSHS"
     gene            1522..4662
                     /locus_tag="VKL21_07365"
     CDS             1522..4662
                     /locus_tag="VKL21_07365"
                     /inference="COORDINATES: protein motif:HMM:NF023335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HMB45624.1"
                     /translation="MYNGIYERLISRLVESELKTVNREQFFVKESSIEKSEASKLLSQ
                     YLSEVINCALGLITDENSIEQQIEVSNKIILLLKDELKNIDFEDDLIAVNGRILNAIF
                     SKLNSNVVNYEEHLKKITPFTRLTQSELFTGSNIGISLESELKKEILSSDKIYFLVSF
                     IKWSGIRIFENELREFTQKGNKLKVITTSYMGATDLKAVEFLSGLPNTEVKISYNTEN
                     ERLHAKSYLFFRNTGFHTGYIGSSNISRSALTSGLEWNLKITTKEIGHIIDKFEKTFE
                     TYWQDNEFETFIKEEHIEKLRTALKKERKNDRTETTFYFEIKPFPFQQEILEKLEVER
                     TIHHRNKNLVVAATGTGKTIISAFDFKNYLKKNPNAKFLFVAHRKDILIQARTMFAGV
                     LRENNFGELWVDGEEPSNYKAVFASVMTLKNRIGQLQLTENYYDFIIIDEVHHIAANS
                     YRPILDKFKPVILLGLTATPERNDGANILDDFCNTIAAEIRLPEALNRKLLSPFQYFG
                     ISDSVDLSNATWQNGRYLPSELSRLYGQNDIRIGEIIQKCKGYLRDFEDVRAIGFCVT
                     KEHASYMAEKFVLAGLKADFIVSGNGRDELRESIKSKFQKKEINYLFVVDIFNEGIDI
                     PEIDTVLFLRPTESLTVFLQQLGRGLRLADNKECLTVLDFVGNARPEYDFEGKFRALI
                     GKTNSSTMEELEREFPHLPLGCSIVLEKKAKEYILENIKNATQLNTNQLINKIINFTH
                     QTTLPLNLKNFCEFYQILIQSIYKRGNWSRLCQLANQRADFPNTNEKEVNRTILKKWI
                     QCNSYSYLKFILELAKNNFSIPSTINEKEKLMLVMLHYDFWQTAENFDNIEKSIKKIG
                     ENQILTQEMIETLEFLIDRISHIELDIDLPYSQPLKLHSRYTRDQILAAFGANTFQKQ
                     SSNREGLVNLESKNTELLLVTLEKTEDNYSPTTMYNDYAVSEKIFHWQSQNSSKPEIG
                     RGLSYITHKEKGKSILLFVREKNTDEFGNTMSYVFLGAVNYIEHYGSKPMSIKWDLQV
                     PIPPYIWKETAKMAVG"
     gene            complement(4905..5294)
                     /locus_tag="VKL21_07370"
     CDS             complement(4905..5294)
                     /locus_tag="VKL21_07370"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMB45625.1"
                     /translation="MVKIEGICKEVLDKTEWVAIATCGDSGPHIVATWGGYVKALGTN
                     DSGIIVIPAGYYHITEENLKKNNRVELLIATRQVNGTNGHPGQGCSISGRGEIQTSGK
                     FADMAKSKFSWARGALVITVEKVSTQL"
     gene            5748..5996
                     /locus_tag="VKL21_07375"
     CDS             5748..5996
                     /locus_tag="VKL21_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45626.1"
                     /translation="MSLLTDGKLEEARLILEDLAFRSPKDPNVLYNLGMVYSEFHELD
                     IAIDILNRCVNIVPLYSNAYVALGVAYLLADQPSWIEA"
     gene            complement(6050..7192)
                     /locus_tag="VKL21_07380"
     CDS             complement(6050..7192)
                     /locus_tag="VKL21_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMB45627.1"
                     /translation="MLFRKMNKVEYGLSILGFGCMRLPVTKEGNIDENQATDMLRYAI
                     DHGVNYVDTAYPYHNGESEPFVGRALQGEYREKVNLATKLPSWLIKSRADMDRYLDEQ
                     LKRLQTDHIDFYLVHGLMKPFWENLSTLGVTDFLDSAIADGRIKYAGFSFHDELALFK
                     EIVDAYDWTFCQIQYNFMDEQNQAGTEGLRYAADHGLGIVIMEPLRGGMLTRDIPSIN
                     NIWKKAPVQRSLPEWALRWVWNHPEVTVVLSGMSSFEQVEQNVGYAKSGLPNSLSQEE
                     LSFFKEVEIEYKKRIKIPCTGCRYCMPCSSNVSIPECFEMYNQGCMFEAPDTASLQYD
                     FALSGFLTGSPGFASQCQECGECEEKCPQGIPIQEYLKKVAAYFGK"
     gene            7534..8073
                     /locus_tag="VKL21_07385"
     CDS             7534..8073
                     /locus_tag="VKL21_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMB45628.1"
                     /translation="MDVTDALNSRNSTRAFKPDPVDKENITRILETATRTPSWANTQP
                     WEIFVAGGDVLNRLRNAYLENYHKDVPGRSDLPRPQQWPPALQKRMEELGAERFKTAG
                     IKRDDKAARQAIFETNYRFFGAPVVIYLCTIASRYRWKTTLIFDSIYLQILFSAFRIY
                     YVFIHIISLKTIFKYETES"
BASE COUNT         2723 a         1437 c         1512 g         2411 t
ORIGIN      
        1 atacttttac aagtttgtca gggtcaataa aagaaaattc tttggttttg agaaaataaa
       61 gatatcggat gccgagatta acattgcaga caaggaaaaa gcaataatcg attgtcttga
      121 cagggaagaa tacgcgggtt ctgtcataga aatattaaaa gcactccaca acaacaaaga
      181 gcagtttgat ttcgacaaga tgataaacta tgccctgata atggaaaatc attcgctggc
      241 aagaaggctt ggcttcctgc ttgatcttgt aaaacaggat acaaaaaagc ttgaagaaag
      301 gatcggaaaa ttcaggtatg tttatttgtc aaacaccctg ccaaaaaaga aacatggaat
      361 aagcaagaaa tggaggctta ttttgaacct ggaagaaaac gagttgttta aatggtaact
      421 aaagcagagc tagtcagcat agcaaggaag caaaagcttg cataaactct acctgcacaa
      481 aaggctatct gttgaccttg attttacagc attggatgaa ttcgatattg atattttcaa
      541 gaatatttta ttgataaaag agataaattc gcaaatccga aaaatcagag catttgaaaa
      601 ttctgtttcg atagatttga aatacattta gagaaaaagg cccagggatt atttagatat
      661 gtggcttttt ctgaaagaac ttgaattcaa ggattttgaa aagcttgcag agaaaaagct
      721 ggctgccatg aacgataata tggatattgg taaagttttt gctgatctgg atgtcgtaaa
      781 atctctatgg aaagttgatc tggagcagct tattccggaa ctgccggatt tcgatggtgt
      841 tatgaacgat ttgaagaaga aacttgattt gcattaacaa agtcctgact ttatattcag
      901 gaatgggagg agggagtctg acaaatagta accactcttt ccccgtcttt caatttttta
      961 actaaatgaa tatcagctgg tgcaaaatcg taataatcca attccgttaa gggaacccat
     1021 ttatattcat catgtgccat taattctata ctaccgcttt cccaaaatac ccaatatgcc
     1081 aataattgaa ttgcaccttg agggtattga taaatacttt ctccaaaaaa gtctccaatt
     1141 ctcacattaa tttttagttc ttcttcaatt tcccttttta aacattcttc tgctgtttcg
     1201 ccattttcaa tttttccgcc aggaaactcc catttattag ctaacgtatc tgccttcttt
     1261 ctttttgcta taaaaaaaag accatcttta attatgatag ctgcagtaac ttttttcata
     1321 ttaagcgctt ctattaatat tttgatatta taaattctcc ttttcgtaga ggggtcagaa
     1381 aagatataaa agaagtttta aataataaaa tagagaaact aagatcatat ggaaaagtat
     1441 atcgaaaaat gaatgatatg ggaataagtt catgtggtta atgaaagttt ggaattatta
     1501 gagaaggtta catcttaaat tatgtataac ggtatttacg aacggctaat aagcaggtta
     1561 gtcgaatcag aattaaaaac agtaaataga gagcagttct ttgttaagga atcctcaata
     1621 gaaaaatcag aggcttctaa actacttagc caatatttga gtgaagtaat aaactgtgct
     1681 ttgggtttga ttacagatga aaacagcatt gaacaacaaa tagaagtttc aaacaaaatt
     1741 atactacttt taaaggacga gttaaaaaat atagattttg aagatgattt gatagccgta
     1801 aatggaagaa tattaaatgc tattttttca aaattgaatt caaatgtggt aaattatgaa
     1861 gaacatttaa aaaaaattac accatttaca cggcttactc aaagcgaatt atttacaggt
     1921 tcaaacattg gtatttcact agaaagtgaa ttgaagaaag aaatcctttc atcagataaa
     1981 atttatttct tagtttcatt cataaaatgg tctggaatac ggatttttga aaacgaatta
     2041 agagagttta ctcaaaaggg aaacaaattg aaagttataa caacttcata tatgggagca
     2101 accgatttga aagctgttga gtttctatca ggattgccaa atactgaagt aaaaatatct
     2161 tacaatacag aaaatgaaag gctgcatgcc aagtcttatc tattctttag aaatacaggt
     2221 tttcatacag gatacattgg ctcctcgaat atttcaagaa gcgctttaac gagtggttta
     2281 gaatggaatt taaaaataac cacaaaagaa attggacaca ttattgataa atttgaaaaa
     2341 acatttgaaa catattggca agataacgag tttgaaactt ttattaaaga agagcatatt
     2401 gaaaaattac gcacagcttt aaaaaaagaa agaaaaaatg atagaactga aacaacattc
     2461 tattttgaaa taaaaccatt tccatttcaa caagaaatac ttgaaaaatt agaagtggaa
     2521 agaacaattc atcatagaaa taaaaatctt gtagtagctg caacaggaac aggtaaaacc
     2581 attatttcag cctttgactt caaaaattat ctgaagaaga atccaaacgc aaaatttctt
     2641 tttgttgctc atagaaagga tatcttgatt caagcaagaa caatgtttgc gggtgtattg
     2701 agagagaata attttggtga gttgtgggtt gacggagagg aaccaagtaa ttataaagct
     2761 gtatttgcct ctgttatgac tttaaaaaat cgaataggtc agttacaact aacagaaaac
     2821 tattatgatt ttattattat tgatgaagtt catcatatag cagcaaatag ttacagacct
     2881 attttagata aatttaaacc agtaatatta ttgggattaa ccgcaactcc tgaaagaaat
     2941 gatggtgcaa atattttaga cgatttttgt aatactattg ctgctgaaat tagattgccg
     3001 gaagctttga atagaaaact tctttctcca tttcaatact ttggtataag tgacagcgtt
     3061 gatttgtcaa atgcaacttg gcaaaatggt agatatttac caagtgaact ttcaaggctt
     3121 tatggacaaa atgatataag aattggtgaa attattcaga aatgcaaagg ctatcttaga
     3181 gactttgaag atgttagagc cataggattt tgcgtaacaa aagaacatgc atcttatatg
     3241 gctgaaaagt ttgtcttagc tggtttaaaa gctgacttta tagttagtgg taatggaaga
     3301 gatgaattaa gggaaagcat taaatcgaaa tttcaaaaaa aggaaatcaa ttatttattt
     3361 gtcgttgata ttttcaatga aggtattgat atccctgaaa tagatactgt attgttttta
     3421 agaccaactg aaagtttaac tgttttcttg cagcaattag gacgggggct aagattagca
     3481 gataataaag aatgtctaac cgtattagat tttgttggaa acgcaagacc tgaatatgat
     3541 ttcgaaggta aatttagagc actgatcgga aaaacaaact cttcaactat ggaagaatta
     3601 gaaagagaat tccctcattt gccgttaggc tgttctatcg ttttagagaa aaaagctaaa
     3661 gagtatatcc ttgagaatat taaaaatgca acacagctaa acacaaatca gctaatcaac
     3721 aaaattatta attttacaca tcagacgacg ctaccattaa acttgaaaaa cttctgtgaa
     3781 ttttaccaaa ttcttattca atcaatttat aaaagaggta attggagcag gttatgtcaa
     3841 ttagcaaatc aaagagctga ttttcctaat actaatgaaa aagaagttaa tcgaaccatt
     3901 ttgaaaaaat ggattcaatg taattcatac agttatttaa agttcatatt ggaactcgca
     3961 aaaaataatt tttctattcc ttcaacaata aatgaaaaag aaaaactaat gttagtgatg
     4021 ttacattatg atttttggca aacggcagaa aattttgaca acatagaaaa gagcataaaa
     4081 aaaattggcg aaaatcaaat tttgacacaa gaaatgatag agactttaga gtttttgatc
     4141 gacagaatat ctcatataga acttgatatt gatctacctt attcacaacc tttaaaatta
     4201 catagccgct atacacgaga tcaaattttg gcagcatttg gtgcaaacac ctttcaaaaa
     4261 caatcaagta atcgagaagg acttgttaat ttggaatcaa agaatacaga attgctctta
     4321 gttactttgg aaaaaacgga ggacaattat tcgccaacaa ctatgtacaa tgattatgca
     4381 gttagcgaaa aaatatttca ttggcaatct caaaattcat caaaaccaga aataggtaga
     4441 ggtttatcat atattactca taaagaaaaa ggcaaatcaa ttttactttt tgtaagagaa
     4501 aaaaacacag atgaatttgg aaacacaatg tcttatgttt ttttaggggc tgtaaactac
     4561 atagaacatt atggctcaaa gccaatgagc attaagtggg atttacaggt gccaatccct
     4621 ccttacatct ggaaagaaac tgcaaaaatg gctgtgggtt gatcaagtag ctcctgttgg
     4681 agtggtgtag ctggagataa tagaatcccc ggcgtaatag aatagggcag gaatttcttt
     4741 tacgctaact tatgacggtt tcagtcaccg ccgcccacgt actatgttcg aggcctgtat
     4801 aggatgcatc gacccgtatt gaggtaataa ttttttctat cgcaacatac cttgatcaga
     4861 gcagcgttcc atgtcgtttg cacctggaat tcacgcacat gttactacag ttgtgtgcta
     4921 accttttcca ccgtaataac caacgcccct cttgcccagg aaaatttcga ttttgccata
     4981 tctgcgaatt ttcctgaagt ttgaatttct cctcttcctg agatactaca tccctgtcct
     5041 gggtggccat tggttccatt gacttgcctt gtagcgatca gcaactccac ccggttattc
     5101 ttttttagat tctcctcggt tatatgataa tatccagctg gaataacgat tattccacta
     5161 tcattagttc ctaatgcttt cacataacct ccccatgtcg ccactatatg tggaccgcta
     5221 tcaccacagg ttgcaatagc aacccattct gttttatcta aaacttcttt gcatatccct
     5281 tcaattttta ccatatcagt tcctccattg gattagattt ctaatttcaa tactccctta
     5341 attacatcac gcttatgata tttaaataat tgtatctggc aataaaacgt acgaaatttt
     5401 taatccatat aaaaatcata cactataaac ataaaatatt aattatgtat gtattttcaa
     5461 atatgtaaag aactttcata aactactcat ttgaaaatcc cctcccccgt cctatgtctg
     5521 aggcctgtac atgatgcaac gacccgtatt aaggttatat tattttctat cgcaacatcc
     5581 cttaatctga gcagccaggc gtgggtcata actgatgctg ggcacggtgt tgccgggatt
     5641 aggggtttct atggagaata tcatagtgca tacagaatga catcataacg atcgaatgga
     5701 gccccctgga aaaagatgaa tatttcgatg ctgagttcca gaggtcaatg agccttctca
     5761 cggatggcaa attagaggag gcaagactca tcctggagga tctggcgttc cgatcgccca
     5821 aagatccgaa cgtgctttac aatctgggga tggtttacag cgagttccat gaacttgata
     5881 tagcaattga tatcctgaac aggtgcgtaa atattgttcc tctatactcc aatgcctatg
     5941 ttgcgctggg ggtggcttac ttactcgctg accagccctc ctggatcgaa gcatgaacct
     6001 tgaggagcgc agagcaatct gcaccccaat gtgagtcttt gtgaagtaat cattttccga
     6061 agtaggctgc gaccttcttg aggtattctt gaatcggtat gccctgtgga cacttctcct
     6121 cgcattcccc gcattcctgg cattgggatg cgaatccagg gcttccggtt agaaaaccac
     6181 tcaatgcaaa atcgtattgg aggctggcag tgtcaggcgc ttcgaacatg cagccttgat
     6241 tgtacatctc aaagcactca gggatgctaa cattggatga acatggcata cagtatctgc
     6301 agcctgtgca tggaatcttg atgcgcttct tatactcgat ctccacctct ttaaaaaagc
     6361 taagttcctc ttgagaaagc gagttaggaa gaccactctt tgcatatccg acgttttgct
     6421 caacctgttc gaaggaagac atgccggata aaacaactgt cacttcagga tgattccata
     6481 cccaccgcag ggcccattct ggaaggcttc tctggacagg agcctttttc cagatgttgt
     6541 ttatggaagg aatgtccctg gtgagcatac caccacgcaa aggctccatg atcacaattc
     6601 caagtccatg gtcagctgca tacctgagtc cctctgttcc agcttgattc tgctcatcca
     6661 tgaaattgta ttggatctgg cagaacgtcc aatcgtatgc atctacaatc tccttaaata
     6721 atgccagttc gtcgtggaat gagaaacctg catacttaat tcttccatcg gcaatggcac
     6781 tatccaggaa gtcggtaaca cccaatgtac tgagattttc ccagaatggt ttcatgagtc
     6841 cgtggaccag gtaaaaatcg atatgatctg tctgtaaccg cttgagctgc tcatcaagat
     6901 aacgatccat atcagcacgt gatttgatca gccagctggg aagcttggta gccaggttca
     6961 ccttctcgcg atattctccc tgcagtgctc gaccaacgaa aggttcgctc tccccattat
     7021 ggtatggata ggcggtgtca acgtagttca cgccatgatc tatggcatac ctgagcatat
     7081 cggtagcctg gttttcatcg atatttccct cttttgttac tggcaggcgc atgcatccga
     7141 agcccagtat agagagacca tattccactt tattcatttt tctaaatagc attgtctcat
     7201 ctcagatttt gcatattctc attctcgaca tcctcactat cgaataaaat gatattcaat
     7261 gtaagtgaga catacatgag tatgggtaat aatggtatcg caatgatttc tacgtacgct
     7321 aagaaattag cacctcacta aatctctatt gaactattcg gagggaagat atgtacaaca
     7381 ttgaatgatt gataacttat attatacact ctgaaggaca gccgggaaga gcaaaacttt
     7441 taaacgaaag gacaatatga gaattaattc aataattgtc attacagaat atctccggct
     7501 ttcccgccgg taactaataa ggaggtaaat tttatggatg ttacagatgc cctaaattcc
     7561 cgtaatagca cccgagcttt taaacccgat ccagtcgata aggaaaacat caccaggata
     7621 ctggaaacag caacccgaac accctcatgg gccaatacac agccatggga aatattcgtt
     7681 gccggcgggg atgttttgaa ccggctgcgc aatgcctact tagaaaatta ccataaagat
     7741 gtaccgggca gatcggatct accaagaccg cagcaatggc caccggccct gcaaaaacgc
     7801 atggaagaac tcggagcgga gcgatttaaa actgctggta taaagcgaga tgataaagct
     7861 gcaaggcaag cgatctttga gacaaactat aggtttttcg gggcgccagt ggtcatatac
     7921 ctgtgcacta tagcatcgag ataccgttgg aaaacaacgc taatatttga tagtatctat
     7981 ctacagatat tgttttctgc atttaggata tactatgtat ttatacatat tatatcatta
     8041 aaaactattt ttaaatatga aactgagagt tagttttgag tta
//
LOCUS       DATGBN010000302         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_334474, whole genome shotgun sequence.
ACCESSION   DATGBN010000302 DATGBN010000000
VERSION     DATGBN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334474"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VKL21_07390"
     CDS             <1..607
                     /locus_tag="VKL21_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45629.1"
                     /translation="EGVVYMDPMQSIARGANFDMQISYALIPRDQKYVFNKSTIYNTT
                     SISMFIDKKSGIDPEGSSEVMILSGNEYNVIAFYNLRAGEMISIPVKIIPEYSGYLYT
                     GIGLFFLFSIALIYLLKEKILKRRKKESTLEELEIEKRNIYMTIHGFEKHSGPEISEE
                     YRELMEEYRQKAIRICIKIDNFKKKQTGKMSVMMSKGCSNK"
     gene            691..882
                     /locus_tag="VKL21_07395"
     CDS             691..882
                     /locus_tag="VKL21_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HMB45630.1"
                     /translation="MQIDPICGMQVDENSAQYKSEYKGNTYYFCAPGCKKAFDSDPEK
                     YQKGGPVGMDEKKPWWKFW"
     gene            887..1324
                     /locus_tag="VKL21_07400"
     CDS             887..1324
                     /locus_tag="VKL21_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45631.1"
                     /translation="MRISGIIIALLILIMAAVSGCLSGKEPQTENPAAVPTSSDKTST
                     VDSSETKVNSEAGVIITAVNLGNNAFDIKLDTHSGSLDYDMAKLSYIRDSKGNIIKPE
                     SWDGGIGGHHFEGTLKFPEFDDSAGFELVIQDVAGVKERVLKW"
     gene            1318..1821
                     /locus_tag="VKL21_07405"
     CDS             1318..1821
                     /locus_tag="VKL21_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45632.1"
                     /translation="MVNKMSIKQVIKNLLKNPFVFGASAGMLLVLFNISIASLAEGSF
                     EKGYQVFLTNGIFVYLIPLAVSVQMVLFRYHRNITTGNIAGSEKMGMAGSATSSLTMV
                     ACCLHHVSDILPSIGLILAASSFLIQYKDAIIIIGLLANVAGSIYIARAIIKDRSIIA
                     KVEKIAA"
     gene            1832..2434
                     /locus_tag="VKL21_07410"
     CDS             1832..2434
                     /locus_tag="VKL21_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45633.1"
                     /translation="MKKTHRVIAIVLIVTLAASFLYLSDSRKSIFTDKVGDMTLSGYD
                     TGDVAAQQISKMYGIKDIPLEKAYSAVYRSNRGTMRIWAVEAPDHNAVNDAFNAMNSM
                     LGGPSGNDEHGNSGNMGDMGSGNMDSNMDMMNNNFTKPVKLDIMEFEKPDVYVMKENN
                     VINYYYFKMNYNRGRVYWIIFDSPDLDYQISIVKQAVMEI"
     gene            2443..3225
                     /locus_tag="VKL21_07415"
     CDS             2443..3225
                     /locus_tag="VKL21_07415"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMB45634.1"
                     /translation="MRKSFSNLIALGFIALMALALLSGISVLLGYEKPGNIEGGGHHL
                     FTGYAYGNWGATLLSVGIFSVFVLTFLTPLKKQNWRTLGIYEAFIIALFSEMYGFPLT
                     IYVLSSLFGLNLSFGHAQGHLLGVLISKAGLMSMDAAWALVMVTSSAVIFLGLFLMSK
                     GWSMIHASSGELVTGGIYKYVRHPQYLGLIILTVGLLIQWPTIITLAMWPVLVLMYYR
                     LAKREEREAIEAFGEIYEEYMRQTPMFLPSLRKKGMASQVIL"
BASE COUNT         1094 a          623 c          789 g          977 t
ORIGIN      
        1 tgaaggggtg gtatatatgg atcccatgca gtcgatcgcg cggggcgcta actttgatat
       61 gcagatatca tatgcactta ttccccggga tcaaaaatat gttttcaata aaagcactat
      121 atataatact acttctattt cgatgttcat tgataaaaaa agtggaattg acccggaagg
      181 atcatctgag gttatgatac tttcaggaaa tgaatacaat gttattgctt tttataatct
      241 cagggcaggg gaaatgatca gcattcctgt aaaaataatc cctgaatatt caggttattt
      301 gtatacagga attggattat tctttttatt ttcaatcgcc ttaatttatt tattaaagga
      361 aaaaatctta aagagaagaa aaaaagagtc cacgcttgaa gaacttgaga tcgagaaaag
      421 aaatatttac atgacaattc atggatttga aaaacattca ggacctgaaa tctcggaaga
      481 ataccgggaa ttgatggaag aatacaggca gaaagccatc cggatttgta ttaagatcga
      541 taactttaaa aaaaaacaaa caggaaagat gtcagtgatg atgagtaagg ggtgttcgaa
      601 caaataaatt gcttccgtca caagatacct actttacacc aaacgtaaag tacatataca
      661 gtaaaaacaa taaaaggaga aatgataaat atgcaaatag acccaatttg tggaatgcag
      721 gtagatgaaa attcagcaca gtacaagtca gaatataaag gaaatactta ttatttctgt
      781 gcgcccggat gtaagaaagc atttgattct gatcccgaaa aataccagaa ggggggccct
      841 gtgggtatgg atgagaaaaa accatggtgg aagttctggt gattacatga gaatatccgg
      901 aatcataata gcgcttttga tcttgattat ggcagcagtt agcgggtgtt tatcaggaaa
      961 agaaccacaa actgaaaatc ctgctgcagt tccaacttct tcagataaaa catctacagt
     1021 ggattcatct gaaacaaaag taaactcaga agcaggagta attatcactg cggtgaatct
     1081 tggaaacaat gcctttgata tcaaactcga cacacattca ggatcgctgg attatgatat
     1141 ggccaaatta tcctatatcc gtgatagcaa gggcaatatt ataaaaccgg agtcatggga
     1201 cggaggcatc ggagggcatc attttgaagg cacgctgaaa ttcccggaat ttgatgatag
     1261 cgctggtttt gaactcgtta tccaggatgt tgcaggagtg aaagaaagag tcctgaaatg
     1321 gtaaataaaa tgtcaataaa acaggtcatc aaaaacctgt taaaaaatcc ctttgttttc
     1381 ggggcaagtg caggaatgct tctggttctt ttcaatatct cgattgcttc ccttgccgaa
     1441 ggatcgtttg agaagggata ccaggtattt cttactaacg gaatatttgt atacctgatc
     1501 cctcttgcgg tcagtgtaca gatggtgctt ttcaggtatc acaggaatat tacaaccgga
     1561 aatatagcag gctcggaaaa aatgggtatg gcaggttcag ctacttcatc gctgacgatg
     1621 gtggcatgct gcctgcacca tgtgagcgat attttgccct caattggttt aatccttgcg
     1681 gcatcatcgt tcctcatcca gtataaagac gcaataataa tcatcggact ccttgccaac
     1741 gtagctggaa gcatctacat tgcaagagcg atcataaaag acaggtcaat aatcgcaaaa
     1801 gtggaaaaaa tcgcagcctg agggcaaaaa aatgaaaaaa actcatcgtg taattgcaat
     1861 cgtccttatc gtcacgctgg cggcatcatt tctatattta tctgactcca ggaaaagcat
     1921 cttcactgat aaagtgggag atatgaccct ttccggatac gataccggag atgtggcagc
     1981 gcagcagata tcgaaaatgt acggtatcaa ggacataccg cttgaaaaag cttactcggc
     2041 agtgtacagg agcaataggg gcacaatgag aatctgggcg gtagaagctc cggaccataa
     2101 tgctgttaac gatgctttca atgccatgaa ctcaatgctg ggtggcccat cgggaaatga
     2161 tgagcatgga aactcaggca atatgggaga tatggggtcg ggtaacatgg acagcaatat
     2221 ggatatgatg aacaataact tcactaaacc agtaaaattg gatataatgg agtttgaaaa
     2281 acctgatgtt tatgtgatga aagaaaataa tgtaataaat tactattatt ttaagatgaa
     2341 ttacaaccgg ggaagagtct actggataat ttttgattca cctgatcttg attatcagat
     2401 atcgattgtg aagcaggcag taatggagat ataggtgaaa ttatgagaaa atcattcagc
     2461 aaccttattg cgcttggatt tattgcgctc atggctttag ctctgctttc aggtatatca
     2521 gtgcttcttg gttacgaaaa acctggcaat atcgaaggtg gagggcatca tttatttaca
     2581 gggtatgctt acggcaactg gggagcaaca cttcttagcg ttgggatttt ttctgtcttt
     2641 gtgctaacct tccttactcc tctgaagaag cagaactggc gtaccctggg gatatatgaa
     2701 gccttcataa tagcgctgtt ctctgaaatg tacggcttcc cgcttacgat atatgttctc
     2761 tcttcacttt tcggactgaa cctttctttt gggcatgcac aggggcacct gcttggagtg
     2821 ctcatttcca aagcgggttt aatgagtatg gatgctgcct gggcacttgt gatggtaaca
     2881 agcagcgcgg ttatattcct gggactattc ctgatgtcaa agggctggag catgatacat
     2941 gcatcttccg gagaactcgt gacaggcggg atttacaaat atgtcaggca tccgcagtat
     3001 cttggtctta ttatattgac tgttggcctg ctcatccagt ggccgaccat aatcactctt
     3061 gcaatgtggc cagttcttgt tttaatgtat tacaggctgg caaaaagaga agaaagagaa
     3121 gcgatagaag ctttcggtga aatatacgaa gagtacatgc ggcagacgcc tatgtttttg
     3181 ccatccctga gaaagaaagg tatggcaagt caggtgatat tataacacta acgctctgga
     3241 tatccctcgg atttctggtt attgcatcgt ttcttccgaa attttttaat tcaaggttca
     3301 tattcggtgg ttccggctgg atattccttg caatatactg gtccgggcag cttgggggtt
     3361 atattgagct taaggattat gttaatatta tcctggtaat tgccgcaacc tttgcatcct
     3421 tatttataac acatataaca ttccaggcga aggataataa taataaaggc gactatgaag
     3481 tgt
//
LOCUS       DATGBN010000303         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_257002, whole genome shotgun sequence.
ACCESSION   DATGBN010000303 DATGBN010000000
VERSION     DATGBN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257002"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VKL21_07420"
     CDS             <1..642
                     /locus_tag="VKL21_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HMB45635.1"
                     /translation="LKELALTYEYAFDSDDKQIFLNNELERISKELRVIYRNELSMVD
                     DATLEKSVTDVRPLIGTISSNLSLNGDFYSNEFIQDETILRSDKFGISGSLDRLLKKN
                     ESMVPSIIKTGNMPDNGIWNSERLALTTYSILVEEKYNSIIEKGFVEYARWGKVRPVV
                     IKRHERRQVLRIRDKIKKIQDGFMPERPEYAPCDYCNFVGICDVKSTLASRFF"
     gene            complement(682..915)
                     /locus_tag="VKL21_07425"
     CDS             complement(682..915)
                     /locus_tag="VKL21_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HMB45636.1"
                     /translation="MAVGFVLINVTPGAEKKIFDTMIKWEEIQELYPLFGDYDLIAKI
                     QASDYEGLSEIVVNKIRALKGVTETKTLTGAKF"
     gene            complement(988..1716)
                     /locus_tag="VKL21_07430"
     CDS             complement(988..1716)
                     /locus_tag="VKL21_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45637.1"
                     /translation="MKFAEKCPKCGGFVQTKSVRKSIGLGFIEIPIAQFCLNPVCEWY
                     QDFAETKKPEDIKEGFQLKIPSMTGFKKPEFNIPEISKNQMIAISATIGLIIISALFS
                     LFVYDTGGDVNQKPQPSQSEFQNITPTVSSQTPNIPVPTVVSTSAIIDPKSYTIRVDV
                     SHGFYPEVITINKYDTIIWSNEENQRTRVVLVSKNSLFEKQLMLYPDRFQYQFNRQGN
                     YSFVLAEYPTNNEYQNAKGNVIVK"
     gene            complement(1782..>2067)
                     /locus_tag="VKL21_07435"
     CDS             complement(1782..>2067)
                     /locus_tag="VKL21_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45638.1"
                     /translation="HCYTILNYKDILIMKILHIIRNPNDTTPLEIAKAQGKDHEVAIL
                     LLHDAVYARTDLTAYASADDAKARGVTGHECVDNKRIVQMLFEYDRIVSW"
BASE COUNT          601 a          392 c          400 g          674 t
ORIGIN      
        1 ctgaaagaac ttgccctgac atatgaatat gcgtttgaca gcgatgataa acagatattt
       61 ctgaataatg aacttgaaag gatttcaaaa gaattacggg tgatctaccg caatgaactg
      121 agcatggttg atgatgcaac acttgaaaaa tccgtaacgg atgtccggcc cttgatcgga
      181 acgatttctt caaatctatc tctgaatggc gatttctatt ctaatgaatt cattcaggac
      241 gaaaccattt tgcgttctga taaattcgga atttccggga gccttgacag gttgctaaaa
      301 aaaaatgaaa gcatggtccc ttcgataatt aaaacaggaa atatgccaga taacggtatc
      361 tggaacagtg aaagacttgc gttgaccacg tattcaattc tcgtggaaga aaaatacaat
      421 tcaattattg aaaaaggatt cgtagagtat gcccgatggg gcaaggtaag acctgtcgtt
      481 atcaaacggc atgagcgcag gcaagttctc cggatcaggg acaagataaa aaaaatccag
      541 gatggtttta tgcccgaaag accagaatat gctccatgcg attattgcaa ttttgttggg
      601 atatgcgatg tgaaatccac gcttgcgtcc aggttctttt gaagacaggt taaatttctc
      661 aaaaatgctt tccaggcact atcagaattt cgcccctgtg agggtttttg tctcggttac
      721 tccctttaat gcccttattt tattgacaac gatctcgctt aatccctcat aatcgctggc
      781 ctggattttc gcgatgaggt cataatcacc aaaaagtgga tagagttcct ggatttcctc
      841 ccattttatc attgtgtcaa agattttttt ttccgctcca ggagttacat ttatcaatac
      901 gaaacctact gccattaatt ttcacctacg acctactatt aggtcatggg aatataatat
      961 tttcctaatt aataaacatg atgattttta ttttacgatc acatttcctt ttgcgttttg
     1021 atattcgttg tttgtgggat attcagccag tacgaatgaa taatttccct gccggttgaa
     1081 ttgatattga aatctgtcag gatacagcat gagttgtttt tcaaataaac tattcttact
     1141 caccagaacc actcttgtcc gctggttttc ttcattgctc cagataatag tatcatattt
     1201 atttattgtt atgacctccg gataaaaacc gtgacttaca tcaactctta ttgtataact
     1261 tttcggatca attatcgcag atgtagaaac tacagtggga accggaatat tgggggtttg
     1321 tgatgatact gttggggtga tattttgaaa ttctgattgt gaaggctgcg gcttctgatt
     1381 aacatcacca cctgtatcat aaacaaataa ggaaaaaagg gcactaatga taattaaacc
     1441 gattgttgcg gaaatggcga tcatctggtt ttttgagatc tcaggtatat tgaattctgg
     1501 ttttttaaac ccggtcatcg atgggatttt taactggaaa ccttccttta tatcttcagg
     1561 tttttttgtc tctgcgaaat cctggtacca ttcacatacc ggattcaggc agaactgcgc
     1621 tatcgggatt tcaataaatc cgagtccgat ggatttcctg acactttttg tctggacaaa
     1681 gccaccgcat tttggacatt tctcagcaaa cttcatgaat actttattgt tagaagcatt
     1741 attaaaatta tcgaatagat ttttgattat aaaaaatcgg attaccacga cacaattcta
     1801 tcatattcaa acagcatctg tactattctc ttattatcaa cgcattcatg ccctgtgaca
     1861 cctcttgctt ttgcgtcatc tgcacttgca taagcagtca gatcggttct tgcatatacc
     1921 gcatcatgca gaagaaggat cgcaacttca tgatctttgc cctgggcttt tgcaatttca
     1981 aggggtgtgg tatcattcgg gttccggata atatggagta tcttcataat tagaatgtct
     2041 ttataattta gaatggtata acaatgt
//
LOCUS       DATGBN010000304         5918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_140638, whole genome shotgun sequence.
ACCESSION   DATGBN010000304 DATGBN010000000
VERSION     DATGBN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140638"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /gene="acs"
                     /locus_tag="VKL21_07440"
     CDS             complement(<1..1456)
                     /gene="acs"
                     /locus_tag="VKL21_07440"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMB45639.1"
                     /translation="MAENPIESLQEEKRIFDPPPEFSKKAYIKSFDEYKEIYKQSIVD
                     PASFWVEKAQQLEWFRKWDKIYFWDPEKAICRWFEGGKLNASSNCLDRHLSTRGDRIA
                     IIWEGDGGENQQFTYKQLYTQVCKFANVLKNKGVKKGDRIAIYLPMIPQLVISMLACA
                     RIGAIHNVVFAGFSADSLKDRIHDSSCTFMITADAGLRGGKTFALKENVDRAINSNDK
                     NPVKTVIVYDRTGFKVNMKEGRDFWWHEEIKNASPECEPEVMDAEDPLFILYTSGSTG
                     KPKGVLHTTGGYLLYTVQTFKWIFDYRDEDLYWCTADIGWITGHSYIVYGPLTSGATS
                     LMFEGVPNYPQPDRFWELVEKFKVTIFYTAPTAIRALERSGDEWPKGRDLSSLRLLGT
                     VGEPINPEAWMWYYNVIGNKRCPIVDTWWQTETGGILITPLPGATPLKPGSATFPFPG
                     IEPMIMKSDGTEAGINEGGQLVIRKPWPGMMRTVY"
     gene            1998..2525
                     /locus_tag="VKL21_07445"
     CDS             1998..2525
                     /locus_tag="VKL21_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45640.1"
                     /translation="MKIRLEIEGKNAGSINYKGTDCIELAAAYLNSIEADEVQVHLVK
                     DDGTKADAEFEGEFPVISAIKFILAASKTRMEVTPGTSIKNGVNESPILTLKERLEMF
                     LRFEYPRVWFTSLDVKKQYEFVYGKIGLSTVSTYLARMSRDNILIRRGNRNQREYRFK
                     DENNIIHNMEFGLVK"
     gene            complement(2884..3135)
                     /locus_tag="VKL21_07450"
     CDS             complement(2884..3135)
                     /locus_tag="VKL21_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45641.1"
                     /translation="MVISVRVCSGRTQFEVSTNINIDLNKASCLFNPKVSTKHLLLLT
                     YKGVDVSIYPSGRMLIKAKTKEESLVVARDILADLGFVI"
     gene            3175..3723
                     /locus_tag="VKL21_07455"
     CDS             3175..3723
                     /locus_tag="VKL21_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein domain-containing protein"
                     /protein_id="HMB45642.1"
                     /translation="MYKGNENNMMGRNVLTLAISVLILALSGSAASDIILNGTGFYLK
                     TGDSFGLSQGYIIKLKSVSIEGSVWLELESDNKIVKSEITNLKGNFTYNKTNRTIISL
                     KVDNIYSGSRDVNLVSFYPVYQYIDPDMPAPRIIKTTPVETPYLDNTSIPALKLVISE
                     ELIWVAVIILILILFYLIRKLW"
     gene            3728..4774
                     /locus_tag="VKL21_07460"
     CDS             3728..4774
                     /locus_tag="VKL21_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HMB45643.1"
                     /translation="MKLLKKNLRGNEGEISLTIESLDDMWHLKYILEPQDIVFAFTKR
                     RIEGATDKLRPEKADKKTVRLGIQVEKVEFHKFSNRLRVHGTIVDGIDAGAYHTINIE
                     EGNEISIIKKWKSDQLERIKEAQIASMRPKVIIATLEEGEASIGLVRQFGVEESSSLR
                     QSLGKGEGNTRNEFFGELASQLKWAAEKVDAVILAGPGFTKEDFFEFIRKKEPELAKK
                     TVLEDTTSIGISGFQEVLRRGAVDRIMEESRIGREALLIEELMKEISINGKVSYGMAD
                     VRNAQSLGAVHTLLITDELLRTEREGKSIDGFLKEVEHSQGKIVVFSTEFEPGKKLES
                     LGGIAALLRFRVNP"
     gene            4771..5433
                     /locus_tag="VKL21_07465"
     CDS             4771..5433
                     /locus_tag="VKL21_07465"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HMB45644.1"
                     /translation="MNIKKATVDIRYLLDNGYPPKSAVGFLCDHYRLDGDSRHILSRT
                     VIAGSICEKRSKKFLPCGKIRGEDIIIDGYNIIIGMESILEKKAILCDDRVVRDIKGV
                     SRNFRSSENTHKAIELILSFLKEKSPSQVFFIFDSQISKSGELAKVLAEKILRSGLKG
                     TAFTSRHVDHDLKCSHYIVASSDSVIIDAAEKVVNFLFCMVCEIPYLEARIAQARNIQ
                     GR"
     gene            5435..>5918
                     /locus_tag="VKL21_07470"
     CDS             5435..>5918
                     /locus_tag="VKL21_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin:alpha-L-glutamate
                     ligase"
                     /protein_id="HMB45645.1"
                     /translation="MKRIGISVTDPTDWTGIAFSNSVTRSGAQALTISFSEATSDLSS
                     GKFYSGKIDLTTLDAIIVRDLGPATRSDVSFRFDILCQLEELGIAVINSPVSILRSAN
                     KYVSSYMFRKNGIATPRTLVTNCREEALLALASFGRAVVKPVFGYKGIGVECVKNDEK
                     G"
BASE COUNT         1776 a         1150 c         1297 g         1695 t
ORIGIN      
        1 catatactgt tctcatcatt ccgggccagg gtttccttat tacaagctgc cccccctcat
       61 tgatccccgc ttcagttcca tccgatttca tgatcatcgg ttctattccg ggaaaaggaa
      121 acgttgcaga accgggctta agaggcgtcg ctccgggaag aggagttatc aggattcccc
      181 cggtctcggt ttgccaccag gtatccacga tagggcatcg cttattcccg ataacattat
      241 agtaccacat ccaggcttcg ggatttatgg gctcgcccac agttccaaga agccgtaagc
      301 ttgaaagatc tctccctttt ggccattcat ccccgcttcg ttcaagcgcc ctgatcgcag
      361 tgggggctgt atagaagatc gtgactttga atttttccac aagttcccag aatcggtcgg
      421 gttggggata attcgggact ccttcaaaca tcaggctggt cgctccgctt gtaagaggac
      481 catacactat ataactgtga ccggttatcc atcctatgtc cgccgtgcac cagtacagat
      541 cttcatcgcg ataatcaaat atccatttga aggtctgcac tgtgtacaat aaatatcccc
      601 cggtagtgtg aagcacacct tttggctttc cggtacttcc gcttgtgtag aggatgaaca
      661 gagggtcttc agcgtccata acttccggtt cgcattcagg acttgcgttt ttaatttctt
      721 catgccacca gaaatcgcga ccctctttca tattcacttt aaagcccgtg cgatcataaa
      781 caataaccgt cttcacaggg tttttgtcat tactgtttat cgccctgtcc acattttcct
      841 tgagcgcaaa agttttccct cctcttaaac cggcatcagc cgtgatcatg aatgtacatg
      901 aactgtcatg gatacggtcc ttgagcgaat ccgcgctaaa tcctgcaaat acaacattgt
      961 gaatggctcc gatccttgcg caggctagca tagaaattac aagctgtggt atcatgggaa
     1021 gataaatcgc tattctatct ccttttttca ctcctttgtt ctttagcacg ttcgcaaatt
     1081 tgcagacttg cgtgtataat tgtttataag tgaactgttg attttcaccg ccatccccct
     1141 cccagatgat agctatcctg tcaccgcgtg tggaaaggtg cctgtcaagg caattggatg
     1201 atgcgttaag ctttccccct tcgaaccagc ggcaaatggc tttttccggg tcccagaaat
     1261 atattttgtc ccatttcctg aaccattcaa gctgttgggc tttttctacc cagaatgatg
     1321 ccggatcaac gatcgattgt ttatatattt ccttatattc atcaaaactt tttatatacg
     1381 cctttttgct gaattcaggg ggcggatcaa agatccgttt ttcctcttgt aaagattcga
     1441 ttgggttttc agccatactg gttcacctgg gcttaatttg gcgcgataat taaaatagtt
     1501 tgtccggttt ttctaataaa atttcgatat atcagattaa aagctgaaaa taaagagatt
     1561 tttgcgcact gtgcttgtga agctcgatgg ctcattataa ttttataata caggcattat
     1621 agacaatatt ttctcaaaaa aattgtaaat aagtgtgtta agctaaaata atgtgtaccc
     1681 cttcgtttcc actggaaaat taatttgaac aaaattatgg cctataatta agtattgagt
     1741 aataataata tctaaatttt aagaaaagtc tataatatat gatattaact aaaaataagc
     1801 aattaaatat ttttatttga tatacatgac cttgaacctt tgtttcaatt gaaaggtata
     1861 tatactaata atacatctgt tcacaatgtc gttcacaaga tgaacactat ttcaccagaa
     1921 ggaaacatcg gaggggaaat ccggatgtaa aactgtccgt ctcgatggat gggcagtctt
     1981 caattttttt gaggattatg aaaataaggc ttgagatcga aggaaaaaat gcgggcagca
     2041 taaattataa aggtactgat tgcatcgaac ttgcagcggc gtatcttaat tcaatagagg
     2101 ctgatgaagt gcaggtacat cttgttaagg atgatggaac aaaagcagat gctgagttcg
     2161 aaggtgaatt ccctgtaata tccgcaataa aattcatact tgcggcttca aaaacaagga
     2221 tggaggtcac acctgggact tctatcaaaa atggtgtcaa tgaatccccg atattgacct
     2281 taaaagagag attggaaatg ttcttgaggt ttgaatatcc cagggtatgg ttcacatcgc
     2341 ttgatgttaa gaaacaatat gagttcgttt acggtaaaat cggcctcagc accgtatcaa
     2401 cctaccttgc aaggatgtcc cgcgataata tacttatccg aagaggcaat cgaaaccaga
     2461 gggaataccg gtttaaagat gaaaataata tcattcacaa tatggaattc ggcctggtaa
     2521 aataatagaa aatatttttt tagaaataat aagcgggttg atgagctgac ctccgggaaa
     2581 ttttcccggg gcctgcagaa cccttgcaac acaattattt gtatttgctt ttctgcagat
     2641 tgcgctccat gcgctttctc atagcacccc aacaggtgaa atcccagtcg agccatttcc
     2701 ttttagacca tgtacggcca caggacaatt acgtccggga aatctggaaa ttcattgtat
     2761 atccaatatg gcttttctgc cgatttcatg ctagctcatc atagcgtgca ccatatcaaa
     2821 caagttcgtg atatggtaaa gtactatttt attttatcat gattaatagt ttttggtaac
     2881 aaattagata acaaaaccca gatccgccaa aatgtccctg gctacaacaa gcgattcttc
     2941 tttggttttt gctttgataa gcatcctgcc gcttggatat attgagacgt ccactccctt
     3001 atatgtcagc aataataagt gctttgttga cactttggga ttgaaaaggc agcttgcttt
     3061 gttcaaatcg atattgatat tggtgctcac ttcaaattgc gtccttcctg agcataccct
     3121 gactgaaata accatatttg ttaattgtga gatgtaaagc aaatatataa ggatgtgtat
     3181 aaaggtaatg aaaacaatat gatgggacga aatgtattaa cattagccat atctgtgcta
     3241 attcttgcac tttcaggatc agcagcaagc gatatcatac tgaacgggac tggtttttac
     3301 cttaaaacgg gagattcctt cggtctttcc caggggtaca taataaaatt aaaaagtgta
     3361 agcattgaag ggtcagtctg gcttgaatta gaatcagata acaaaatcgt aaagagtgag
     3421 atcacaaacc tcaaaggcaa ttttacctac aataagacaa acaggacgat aatttccctt
     3481 aaagtcgaca atatatattc aggttcaaga gatgtgaatc tcgtttcttt ttatcctgtt
     3541 tatcaataca tcgatccaga catgcctgcg ccaagaatta ttaaaacaac acccgtggag
     3601 accccatacc ttgataatac cagtattccc gcgctaaaac tggtaatttc agaagaattg
     3661 atatgggtgg ctgtgattat attaatactt atcctatttt atttaatccg taaattatgg
     3721 tgattgaatg aagctattga aaaaaaattt gcggggaaat gaaggtgaaa tttcactaac
     3781 aatagaatca ttggatgaca tgtggcatct gaaatacata cttgagcccc aggacattgt
     3841 ttttgcgttc acaaaaaggc gtattgaggg agcaacggat aaactcaggc ctgaaaaagc
     3901 tgataagaaa acggtgcgcc tcgggatcca ggtcgagaaa gtagaattcc acaagttctc
     3961 aaaccgcctg cgtgtgcatg gaactattgt tgatgggatt gatgcaggag cataccatac
     4021 aataaatata gaagagggta atgaaatatc tataataaaa aaatggaaga gcgatcagct
     4081 agaaaggatc aaagaagcgc aaatcgcatc catgcgtcca aaagtaatta tcgcaacatt
     4141 agaagaggga gaagcatcca taggacttgt gaggcagttc ggggttgaag aatcatcatc
     4201 tcttcggcaa tcacttggga aaggcgaggg caatacacgc aacgaattct tcggagaact
     4261 tgcttcacag ttaaaatggg cggcagaaaa agttgatgct gtcatacttg ctgggcccgg
     4321 attcacaaaa gaggattttt ttgagttcat aagaaaaaaa gaacctgaac ttgcaaaaaa
     4381 aactgttctt gaggatacga cttcaatagg gatatcggga ttccaggagg tacttcgaag
     4441 aggggcggta gacaggataa tggaagaatc aagaatcgga cgggaagcat tgctcatcga
     4501 ggaattgatg aaagagatat cgatcaacgg gaaagtatcc tatgggatgg cggatgtcag
     4561 gaatgctcaa agccttggcg ccgtacatac cctgttgata acggatgaac tgcttcgtac
     4621 cgaacgggaa ggcaaatcga ttgatggatt tttgaaagaa gtggaacatt cacagggaaa
     4681 aatagttgta tttagcactg agtttgaacc tggaaaaaag ctggaatcac tgggagggat
     4741 agcggcactt ttgcgtttcc gggtgaaccc atgaatataa agaaagcaac cgtcgatatc
     4801 aggtatcttc ttgacaatgg atacccgccc aaaagtgctg tgggattctt gtgcgaccac
     4861 tacaggctgg atggggattc aaggcatata ctttcaagga cagtcatcgc aggttctatc
     4921 tgcgaaaaaa gaagcaaaaa attcctgcca tgcggtaaaa ttcgtgggga ggatattata
     4981 attgacgggt ataatatcat tatcgggatg gaaagtatcc ttgagaagaa ggcaatcctg
     5041 tgcgatgata gggttgtaag ggatataaag ggggtttcca ggaatttcag gagttcagag
     5101 aatacacata aggccattga attgatttta tcgttcttga aagagaaaag cccttcacaa
     5161 gtatttttca tctttgattc ccagatcagt aaaagcggtg aacttgcgaa agtgcttgct
     5221 gaaaaaatcc tccggtcagg attgaaaggg actgctttca cttcaagaca tgtagatcat
     5281 gatttgaagt gttctcatta tattgtggct tcaagtgata gtgtgataat tgatgccgcc
     5341 gaaaaagtcg ttaattttct tttttgcatg gtatgtgaaa tcccttatct tgaagcaagg
     5401 attgcgcaag cgagaaacat tcagggaagg tagaatgaaa aggataggta tctcggtaac
     5461 ggacccgaca gattggaccg gtattgcttt ttcaaattca gttactcgat ctggagcgca
     5521 ggccctgact attagcttca gtgaagcgac ttcagatctt tcatcgggaa aattttattc
     5581 gggaaaaatc gatcttacaa cactggatgc cattatcgta agggatcttg gacctgcgac
     5641 acgaagtgat gtttccttca ggttcgatat tctctgccag cttgaggagc ttggaatcgc
     5701 agtcattaat tcacccgttt ccattctgag atccgccaat aaatatgttt caagttatat
     5761 gttccgtaag aatgggatcg caactccacg aactttggtg acgaattgcc gggaagaagc
     5821 gcttctggca ttggcgtctt tcgggcgcgc cgttgtgaag cctgtgtttg gttataaggg
     5881 aattggcgtg gaatgcgtga aaaacgatga aaagggtg
//
LOCUS       DATGBN010000305         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_412380, whole genome shotgun sequence.
ACCESSION   DATGBN010000305 DATGBN010000000
VERSION     DATGBN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412380"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VKL21_07475"
     CDS             <1..1004
                     /locus_tag="VKL21_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HMB45646.1"
                     /translation="PRKADKLPDFAVTNAMTLKAYKYATEHPEVLEQIPCYCGCSEHG
                     SVTSGGKPHMSVRDCFISNDGVYDDHASFCDVCVGIASKAQSYFPSGIPTTGLSAATP
                     APSIDLSTLSLSENFKSIADGLKLTPEGITRAYFVNTKMIAGTELDIQFLNNQVHPDG
                     FYGKKVIGMFSADYSTNSWIELHDLGYDSKTDGTLKGRTEPGMKNIVITRPLVYGHSQ
                     NVDNVLKLITDPNSMNTSYPAFKPLLDAVDYQNAAYANVIETKNKFSDMNYIGMTPAG
                     GKIELVRAFNITDSKSIPSGLGKYNPETKGNILIIKITGNYTFVTAENDNIDAVAIT"
     gene            1007..1765
                     /locus_tag="VKL21_07480"
     CDS             1007..1765
                     /locus_tag="VKL21_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HMB45647.1"
                     /translation="MSTAPPMLLVFLAGMATVATPCVLPILPAVLSGSVGSRLRPIAI
                     VAGMSLSFTLMGLLVFSVASFGFLTDYLRGFSIFIIIGMGAVLLDDDINEIYVRISSS
                     VINFMREHLSFMGKINSKANPQGLLGGFFLGMSLGILWIPCVGPILGSVFSVVAETGS
                     GNISYGLTLLLTYSLGVSLPMLIIAYSGKSVSGRVKWFSKRSHFFKKLSGLVLILVGL
                     MMLFGIDKYLKILFLPYSINIDNDISRLYEQFFR"
     gene            1768..3186
                     /locus_tag="VKL21_07485"
     CDS             1768..3186
                     /locus_tag="VKL21_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45648.1"
                     /translation="MGTRIDMKDNASSATFGNGKNEKYDLLDIYFFKNLVKNRYFKPV
                     INFLLFIFLLIVIYFGLSSESDLRFHGGIPFATTMIWDIWHPLLAFTVIIVGRLWCFA
                     CPIGAIGEWTQSFFSINRKYPEKYRNLWIAVILFLVIFAGERHLFQFTRNPPNTAYLL
                     LFFTGLAIVMGAVYEKRSFCRYICPIGLVLGVFSMLSAIELRCKSKKTCHDHDIKECV
                     IGSEAGKGCPVGEFPQTMERNNQCIMCMECVKSCSKGNIRISPRIPGADIIHSKKVHL
                     DEAYFVHGLLIIFLFVMGMERLKFRNVIVNFVKSTLPVDSLTFLDLYWRNMWALIIFI
                     LLTLGAAGLMYILTRAVFSGKNTKQKFIELSYAFIPLGLSVYLAENTFRLLKGLFFIV
                     ESIGNLFGRIWEFSPDFDTINLLQLLLLAVGFISTLWAGYIISKRNSKEENEFQQSMI
                     AIGCMAVIYFVIGINILTLPII"
     gene            3280..3762
                     /locus_tag="VKL21_07490"
     CDS             3280..3762
                     /locus_tag="VKL21_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMB45649.1"
                     /translation="MRTKKLISILVLVLLLSFAYFSYKTALSNFTLSDKNSSYLGGIK
                     FHRAASGLEEGFQIAQQENKPVMMYFWAIWCQYCARFQTDTLGNTQVKKILEEDYVLV
                     AMDLDIDREVAQQYGVGYPPYLVFMDPNGKVLERVPGAVEADYLLPIVTKIRDDVRRK
                     "
     gene            3764..>4650
                     /gene="ccsA"
                     /locus_tag="VKL21_07495"
     CDS             3764..>4650
                     /gene="ccsA"
                     /locus_tag="VKL21_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMB45650.1"
                     /translation="MIPPGDIILYASLAVSVIALAGLLLKERNIELLNRLITPAIMAG
                     AGLLTASYLLLTYYFISSNFAYDYVWQYSSADLPLVYKISGTWAGQPGTYLLWVWVVY
                     LSAAWLAWTTRHSTALARRTQVITLITGIYILILTLVQTPFKLIIERPEVVKLIAEGA
                     LTSDFVPLEGNGLNALLVNFWMIVHPPLMFIGYATMTIPFAAAIVYLLTKEDGWEELG
                     RQWTRFTWLFLGIGIAVGGVWAYLVLGWGGFWAWDPVETASFIPWLTLTAFLHAAALH
                     RKNRTTFSIAAPILAAVSF"
BASE COUNT         1325 a          937 c         1017 g         1371 t
ORIGIN      
        1 tcccaagaaa ggccgataag cttccggatt ttgccgttac taacgccatg acattaaaag
       61 catataaata tgcgacagag cacccggaag tacttgaaca gataccctgt tactgcggtt
      121 gcagcgagca tgggagtgta acatcagggg gaaaacctca tatgtctgtt agagattgct
      181 ttatttccaa tgatggggtc tatgacgacc atgcttcttt ctgtgatgta tgtgtcggga
      241 tagcctcaaa agcccagagc tacttccctt ccggaattcc aacaacagga ttatctgctg
      301 ccacacctgc tccatcgatt gatctatcaa ctctctccct gtcagagaat ttcaagtcca
      361 tcgcagatgg cttgaaactg acaccagagg gtattacaag ggcatacttt gtcaatacaa
      421 agatgatcgc tggaacagaa ctggatatcc aatttttaaa taatcaggtg catccagatg
      481 gtttctatgg aaagaaagta atcggcatgt tcagtgcaga ttatagcaca aattcttgga
      541 tcgaacttca cgatcttggc tatgacagca aaaccgatgg aacattaaaa ggaagaacag
      601 aacctggaat gaagaatatc gtaattacaa gaccactggt ttacggacat agccagaatg
      661 tggacaacgt tttgaaatta ataactgacc cgaatagcat gaatacatcg tatcctgcat
      721 tcaaaccatt gcttgatgca gttgattacc agaatgctgc atatgcaaat gtcattgaaa
      781 caaagaacaa attctcggac atgaattaca tcggtatgac cccggcggga ggaaaaatag
      841 aacttgtcag ggcctttaat ataacggatt ccaaatccat tccctcaggt ctgggcaaat
      901 ataatccgga aacaaaagga aatattctca ttatcaaaat aacgggtaac tatactttcg
      961 taacggccga aaacgataat atcgatgcgg tagctataac ataaaaatgt cgaccgctcc
     1021 ccccatgcta ctggtattcc ttgcaggaat ggcaacagtt gctactccat gcgttttgcc
     1081 catcctgcca gcagtgctgt caggttctgt ggggagcagg cttcgcccta ttgccatagt
     1141 tgcgggaatg agcttaagtt tcacccttat ggggcttctt gttttttcag tggcatcatt
     1201 tggttttctt acggattatc tgcgcgggtt ctcgatattt ataataatcg gaatgggtgc
     1261 agtgctgctt gatgatgata ttaatgagat ctatgtaagg atttcaagtt cggtaattaa
     1321 tttcatgcgt gaacacttat cattcatggg aaaaataaat tcaaaagcca atccgcaagg
     1381 ccttcttggc ggtttctttc ttggtatgtc gcttggaatt ctctggatac catgtgttgg
     1441 tccgatactt ggttctgtgt tctcggttgt tgccgaaaca ggatcgggaa atatctccta
     1501 tggtttgacc cttcttttga catattccct gggtgtaagc cttccgatgc ttatcatagc
     1561 atactcgggt aaaagcgtat caggccgtgt caaatggttt tcaaaaagaa gccatttctt
     1621 taagaaactc tcaggtctgg tcttgatcct tgtgggattg atgatgctct ttggcataga
     1681 taaatatctg aagatattgt tccttcctta ttccataaat atcgataatg acatctcaag
     1741 gctttatgaa caattcttca ggtaattatg ggaaccagga tcgatatgaa agataacgca
     1801 agcagcgcaa cctttggcaa tgggaagaac gagaaatatg acctgcttga tatctatttt
     1861 ttcaagaatc tcgtaaagaa cagatatttt aagcccgtta taaatttcct attatttatt
     1921 tttctcctca tcgtaattta cttcggtctt tcaagtgaat ctgacctgag atttcacggg
     1981 ggaatcccat ttgccacgac aatgatatgg gatatctggc atccattgct tgcctttact
     2041 gtaataatcg ttggaagact ctggtgcttt gcctgtccta tcggagcgat cggtgaatgg
     2101 acacagtcct ttttcagtat caacaggaaa tatcctgaaa aatatcgtaa tctgtggata
     2161 gctgtaatac ttttcctggt aattttcgca ggcgagaggc acctgttcca gttcacaaga
     2221 aatcctccga ataccgcata tcttctcctg tttttcacag ggcttgccat agtgatggga
     2281 gcagtctatg aaaaacgaag cttttgccgt tacatttgcc ccatagggct tgtcttgggc
     2341 gtcttttcga tgttatctgc aattgagctt cgatgcaagt caaagaaaac atgccatgat
     2401 catgacatca aagaatgcgt aataggaagc gaggctggaa aaggttgccc tgtgggagag
     2461 tttccccaga caatggagag gaataaccag tgtatcatgt gcatggaatg tgtaaaatca
     2521 tgctcaaaag ggaatatcag gataagtccc agaattcccg gagcggatat catccattcg
     2581 aaaaaagtgc atcttgatga agcttatttt gtccacgggt tactcattat attcctattc
     2641 gttatgggaa tggaacgact aaaattcagg aatgtgatcg tcaattttgt taaatcaact
     2701 cttcctgttg attcactcac tttccttgat ctgtactgga gaaatatgtg ggctctcata
     2761 atattcatac ttttaacgct tggcgcagca ggattgatgt atattttaac aagagcggtt
     2821 ttctcaggaa aaaacacaaa acagaaattt attgaattgt cctatgcatt catacctctt
     2881 ggcctgtccg tctatcttgc tgaaaacaca ttccgcctgt tgaagggatt attcttcatc
     2941 gtggaaagca ttggaaattt atttgggagg atatgggagt tctcacccga ttttgatacc
     3001 atcaatctcc tgcagttatt actgctggcg gtgggtttta tcagtacttt atgggcaggt
     3061 tatattataa gcaagagaaa ctcaaaggag gaaaacgaat tccagcaaag tatgatagca
     3121 ataggatgta tggctgtcat atattttgtt atcggaatca atatattgac attacctata
     3181 atttgaatga ggaaataatt taaaaaaaaa tattaagagg aaaacactga ataaaaacat
     3241 acatgcttat attcgattca aaacattagg aaataacata tgagaacgaa aaaacttata
     3301 tccatacttg tactggtatt actattatct tttgcttatt tcagttataa gaccgcgctt
     3361 agcaatttta cattgagcga taagaacagc agttatctcg ggggcataaa atttcaccgc
     3421 gcagcctccg gacttgagga aggtttccag atcgcacaac aggaaaataa acctgtcatg
     3481 atgtatttct gggcaatatg gtgccagtat tgcgcaagat tccagacaga tacgctggga
     3541 aatacccagg tcaagaagat ccttgaggaa gactatgttc tggtagcaat ggatcttgat
     3601 atcgataggg aagtcgccca gcagtatggg gtgggctatc ctccctatct tgttttcatg
     3661 gacccgaacg gcaaggtcct ggagagagta ccgggtgcgg ttgaagcgga ttatctgctt
     3721 cctattgtta caaaaataag agacgatgta aggagaaaat aaaatgatac ctccaggtga
     3781 cataatactt tatgcaagcc ttgcagtcag cgttattgcg cttgcgggat tgctattgaa
     3841 agaaagaaat attgaattat tgaatcggct tatcacgcca gctataatgg ccggtgcggg
     3901 tttactcacg gcgtcatacc tccttcttac atattacttt atttccagca actttgctta
     3961 tgattatgtc tggcaatact caagtgccga tcttcctctt gtttataaaa tatcaggaac
     4021 gtgggccgga caaccgggta catacctcct ctgggtatgg gtagtttatc tttccgcagc
     4081 ctggcttgca tggactacac gccattcaac tgcgcttgca agaaggaccc aggtgataac
     4141 tcttataaca gggatctata tattaatact tacactcgtt cagaccccat tcaagctgat
     4201 aatcgaaagg cctgaagttg ttaagttgat cgcggaaggg gctttgacat ctgactttgt
     4261 gccccttgaa ggcaatggcc ttaatgcgct tcttgttaat ttctggatga tcgtccatcc
     4321 acctcttatg ttcataggct atgcgactat gacaataccc tttgcggcag caatagtata
     4381 cctgctgaca aaagaagacg gctgggaaga acttggcagg cagtggactc gttttacatg
     4441 gttgttcctt ggtataggta tagcggtcgg gggtgtctgg gcataccttg ttcttggctg
     4501 gggcgggttc tgggcatggg acccggttga gaccgcttca tttataccat ggttaaccct
     4561 tactgctttc ttgcatgccg cggctcttca caggaagaac agaaccacat tttcaatagc
     4621 tgcgccaata cttgccgcag tttcattcat
//
LOCUS       DATGBN010000306         8062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_179417, whole genome shotgun sequence.
ACCESSION   DATGBN010000306 DATGBN010000000
VERSION     DATGBN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179417"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VKL21_07500"
     CDS             <1..187
                     /locus_tag="VKL21_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45651.1"
                     /translation="CETLEYSTLEPESLYQKISTVLNDPGFREKARQYSIMSKTMKGS
                     RKASEIILELSNRIQCY"
     gene            200..730
                     /locus_tag="VKL21_07505"
     CDS             200..730
                     /locus_tag="VKL21_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMB45652.1"
                     /translation="MNFFSYLESVDHSIFLDLNHDFINPFFDSFFPLLRDITYFFWIF
                     LIVYFWVRKEKRLAIILTAGIIAGAIFTYPLKFLIDRQRPYEQLDTTRLLTPFENDPS
                     FPSGHTEMSFLAATIISQFHPRYSKYLYGFSFIVALSRIYVGVHFPIDVIGGAIIGIV
                     IGKLTLRRNLFKSEEI"
     gene            complement(777..980)
                     /locus_tag="VKL21_07510"
     CDS             complement(777..980)
                     /locus_tag="VKL21_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HMB45653.1"
                     /translation="MSSDRKSESRSGGFRPTGPRDMHMGPREMFKTTCADCKQETEVP
                     FKPSGDRPVYCRECYQNHRPKRF"
     gene            complement(1003..1215)
                     /locus_tag="VKL21_07515"
     CDS             complement(1003..1215)
                     /locus_tag="VKL21_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HMB45654.1"
                     /translation="MLTEQDRNSPVSEGAFHDVKIDSIAREGDGIAHIQGLVIFVPGT
                     KIGDQVNIKIERVMPRFAIAALTEKN"
     gene            1411..1731
                     /locus_tag="VKL21_07520"
     CDS             1411..1731
                     /locus_tag="VKL21_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMB45655.1"
                     /translation="MTKSTGKYFYSETAEIHGLYLRKILQLSPSVKLVFKVLEYKGLM
                     TQKELVAESYLPPRTVRYALSILKKEGILEERLYYKDARQCLYGVKTPQHEELLSNFA
                     CGMV"
     gene            1763..2380
                     /locus_tag="VKL21_07525"
     CDS             1763..2380
                     /locus_tag="VKL21_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45656.1"
                     /translation="MDRNSLREIVHLVASLLVLTIAFSYPGTSMEALAIAAIGVGSGF
                     LLHELAHKFTAQRYGYVADYEASPTGLIMAIGLSIFTGGNFVFAAPGAVMIRGKRIAY
                     GQLENYYQDNLANEKEFAYISVSGAVVNLILAVIFIACSFIFADPLTIKVLTRAAFIN
                     VFLAGFNMIPFGPLDGAKVWRYNPVLWGIVGIPSIILFFYLGQLG"
     gene            complement(2537..2701)
                     /locus_tag="VKL21_07530"
     CDS             complement(2537..2701)
                     /locus_tag="VKL21_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45657.1"
                     /translation="MTKNTTAKVIVEWDTGTATGFATKQEAERFVKHICRKTAPNLDY
                     NIYSLNRISG"
     gene            complement(2809..2882)
                     /locus_tag="VKL21_07535"
     tRNA            complement(2809..2882)
                     /locus_tag="VKL21_07535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2845..2847),aa:Arg,seq:ccg)
     gene            complement(2873..4249)
                     /locus_tag="VKL21_07540"
     CDS             complement(2873..4249)
                     /locus_tag="VKL21_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00297 family protein"
                     /protein_id="HMB45658.1"
                     /translation="MWPIKEYEKQSIYILLGFLVLFFPFFKANHFILALLFLITSILL
                     YFVSPDSSFFRFFARESDTRARKLIGMIQLFMTTAILLMISLIVGVDIAGVYKFPLFI
                     IGAAFAITTFGDGIADIIHIIDKEKKQNPNDKQSRHKVYSAKSSMVFLISGSVFAFIS
                     GAWISGLTLYVPVGMLIFLSVIGALTGALLESMTKDEDNIVIPFGSAMVMWLFYLFGY
                     NVDTSYLMNALLFSFVLGYLSYRARVADVSAMLSATLLGVVIIISSDIKWFFILLAFF
                     LLGSMFTRYKYDFKMAHGIAQGKGGVRGYKNVFSNSLAALTLAVAYGIFPSHGQVLMA
                     AYLGSVATACGDTLASEIGETYKGEPRMITTFKKARPGTDGAVSILGEGASFFGASVI
                     AILAFILIPMNLSIVFAVIAGGFIGTNIDSVLGATLQQKGYLTNNGVNLLATISGAIV
                     SGLLYLGS"
     gene            4311..4964
                     /locus_tag="VKL21_07545"
     CDS             4311..4964
                     /locus_tag="VKL21_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HMB45659.1"
                     /translation="MGFISKLYEWNLRTRIRTMNVPHHIVLALSESDLFSDTNYLKLH
                     SFACWCKDMGIKTVTVYISMIEVDTHISNKICSKLSAQIPPALKDITSNINIIKRGDK
                     EIRKHITDDMVLDISLGYSGKYELTRALKEIMSQVKAGTIPPDAINEDVIESHLLFKS
                     EPDIVIRAGGKRLTDFLIWQSVYSELYFTDVNWLDFRKVDFLRVLRDFQRRQRRFGK"
     gene            complement(4984..5928)
                     /gene="uppS"
                     /locus_tag="VKL21_07550"
     CDS             complement(4984..5928)
                     /gene="uppS"
                     /locus_tag="VKL21_07550"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HMB45660.1"
                     /translation="MSLTGLLYSSYEALLANEIKDKPVPAHVAIIMDGNRRFARRRGL
                     AQYYGHFKGADTTEKVLDWSFDIGIKQLTVYAFSTENFDRNDVEKDRLFELIGIKFDK
                     ICQDERTHQRRMRVRVIGNIDLLPPALKLSAKHAEDLTRNYDGVYLNVALAYGGRQEL
                     VDAAQKMAWKVKTGELSLYEINEETISDNLYPSSGSVPYVDLIIRTGGDERISNFLPW
                     QANGNECAAYFCAPFWPEFRRIDLLRAIRTFQTREHERQKNTIMRVVKLLSYYGKVEV
                     EDVIRISRRSITIPKEEVVKILHELANRNVVLNNMIKW"
     gene            complement(5970..>8062)
                     /locus_tag="VKL21_07555"
     CDS             complement(5970..>8062)
                     /locus_tag="VKL21_07555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF002654.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMB45661.1"
                     /translation="LLEGKNIVAAIPTASGKTLLAEFVMLQSVLNENKKGKALYIVPL
                     RALASEKYDRFLGFEAIKKSDGRAVSTGIATGDFDSRDEWLGNFDIIVATSEKVDSLL
                     RNRSHWMEEITVVIADEIHLIDSPDRGPTLEIVLAKLMKQKPQIIALSATIGNAWEVA
                     KWLTAELVVSEWRPIGLKEGVFFGNAVTFGDRSQRHIREFHKDSAISLVADTIKEGGQ
                     CLVFESSRKNSEGMAGRLGMSISNLLDHDAKEKLHVLAAEVRETSEVDTAKKLAMCVE
                     NGSAFHHAGLISEQRKLIEGGFRNGIIKVISSTPTLAAGLNLPARRVIIKGYRRYDVN
                     FGQVPIPVLEYKQMAGRAGRPGLDPYGESVLVAKTYDELDELMKQYVLAGPENIWSKL
                     GSENALRTHILSIIVTGYARNMNELMEFIRATFYSTQQEPWSLKIVIDNIISFLVEKE
                     MIIQKEGLFATRLGEMVSRLYIDPLSASLIIEGMKKIDEKGIDYTELTLLHLLATTPD
                     MRQLYLRSNDYNWLGDLIMDHHTEFLHIPPQFKVDYEWFLSQVKTACVMLDWINEKTE
                     EDIVKKFGIGEGDIRGLSETTLWLVHSMAQLGEFQKRRSAGKARELEKRVEYGASPEL
                     LDLIQIRGIGRVRARKLFDSGIRDLEALRVAQAAKVAKIIGTKVAIKVLKQIGTTPPA
                     EEKLEEQRKLQDFG"
BASE COUNT         2268 a         1782 c         1586 g         2426 t
ORIGIN      
        1 atgcgagact cttgaatata gcacgcttga acccgaatca ctttatcaaa agatcagtac
       61 ggttctaaac gatccaggat tcagggaaaa agcaagacaa tactccataa tgtcaaaaac
      121 aatgaaaggt tcccggaaag catcggagat catcttagaa ttatcaaaca ggatacaatg
      181 ttattgaaac aggcagatta tgaatttttt tagttatctt gaatcagtgg accactcaat
      241 attcctggat ttaaatcatg attttatcaa tccgtttttt gatagctttt ttccgctatt
      301 aagagatata acttatttct tctggatatt tctaattgtt tatttctggg taagaaaaga
      361 aaaaagattg gcaataattt tgaccgccgg tattattgca ggggcgatct ttacttatcc
      421 cttgaaattc ttaattgaca ggcaaagacc atatgaacaa ttagatacaa caagattatt
      481 gacaccgttt gaaaatgacc cctcctttcc atcagggcat acggaaatga gttttcttgc
      541 agctacgatc atctcacaat tccatccccg gtatagtaaa tatctttatg gtttttcttt
      601 tattgtggct ttaagcagga tatacgtagg agtgcatttc ccgatagatg tgatcggcgg
      661 ggcgattata ggaatagtta ttggaaaatt aactttgaga agaaatttat ttaaatcgga
      721 ggaaatctga aaaaaaagga acagtgataa tcctaccacc gcccgttcaa tttttattag
      781 aatctctttg gtctatgatt ctggtaacat tctctgcaat aaaccggcct gtcgccggat
      841 ggcttgaatg gtacttcagt ttcttgttta cagtctgcac aggttgtttt gaacatttct
      901 ctcggaccca tgtgcatatc ccttgggcca gttggcctga atccgccact tcgggattca
      961 ctttttctat cgctactcat ttatatatct ccctttattg ttctagttct tttcagtaag
     1021 tgctgcaatt gcaaatcttg gcataactcg ttctatcttt atatttacct ggtctccgat
     1081 cttcgtgcct ggcacgaaaa taaccagacc ctgtatatgt gcaattccat ctccttccct
     1141 ggcaatgcta tcgatcttga catcgtggaa agcaccttcg gaaacaggag aatttctatc
     1201 ttgttctgtt aacaaatttt tacctctgtt tatttttaac caatctacca aaaaaagcaa
     1261 acacagaggg gaaaccccaa actctataac ttacctaatt agacaactga tatatatcaa
     1321 ttcaacctgt tcatatataa actctttgta gtaccggcat attttgccta aagatttaaa
     1381 tcatatcata acataatccc aagcggtcat atgacaaaat cgactggtaa atatttttat
     1441 tctgaaaccg cagaaatcca tggcctttac ttgaggaaaa tactgcaatt atcaccttca
     1501 gtaaagcttg ttttcaaagt tcttgaatat aagggactca tgacccagaa agaacttgtc
     1561 gcagaaagct accttcctcc ccgcactgta cggtatgccc tgagcatact taagaaggaa
     1621 gggatactgg aagagcgtct ttattataag gacgcacggc aatgccttta cggcgtgaaa
     1681 actcctcagc atgaggagtt attgtcaaat tttgcatgcg gcatggtatg atttatatat
     1741 ttttaagctt aacatgaggc gcatggacag aaacagcctg cgggaaatag tacatcttgt
     1801 ggcgtccctg cttgtgttga ctatcgcgtt ctcttatccc ggtacgagta tggaagcgct
     1861 tgcgatagct gctattgggg taggttcagg tttcctttta catgaacttg cccataaatt
     1921 cacagcccag agatacggtt atgtcgcaga ttacgaagcc agccctacag gattgatcat
     1981 ggctatcggc ctttctattt tcacaggtgg gaatttcgtg tttgcagcgc caggcgctgt
     2041 aatgatccgg gggaaaagaa tagcctacgg tcagcttgag aactattatc aggacaatct
     2101 tgcgaatgaa aaagaatttg cttatatttc agtttcagga gcggtcgtga atttaatact
     2161 tgccgtaata ttcatagcgt gttcatttat ttttgcagat ccgctaacaa taaaagtcct
     2221 cacaagagct gcttttatca atgtgttcct tgcaggcttc aatatgatac ctttcggacc
     2281 gctcgacggg gcaaaagtat ggcgatataa cccggtcctc tggggtattg ttgggatacc
     2341 gtcaatcatc ctgttctttt atttaggaca gcttggatga tcatcaactt tcttgacagg
     2401 atttactgga ttggctcgta ccctgccgaa tggtgccttt gcatgtaatt ttttatgaaa
     2461 ataagtatct gttttgttta taattgaaaa aaaagaattc tatcgaattt gcacatgctc
     2521 aggttatttc attaatttaa ccgcttattc tatttaggct atatatgtta taatccaaat
     2581 ttggcgcggt ttttctgcat atatgcttaa caaaacgttc agcttcctgt tttgttgcga
     2641 atcctgttgc tgttcccgta tcccattcaa ctataacttt ggcggtagta ttttttgtca
     2701 tttaaccctt cctccgtaat acctagttgg cagtctagct atttaaattt atcgaaatga
     2761 tttgataata atggttatgc ttatcatgac aattcttata cacagttacg ggctcgccgg
     2821 gatttgaacc cgggtcctag gctccggagg cccaagtact atccggacta tactacgagc
     2881 ccaaataaag taaaccggat acgatagctc ctgaaatagt tgcaagaaga tttacaccat
     2941 tattcgtgag ataacctttc tgctgaagtg tagcgccaag cacactatcg atgttcgtac
     3001 cgataaatcc tcctgctatc acggcaaata caatggaaag gttcatcggg atcaatatga
     3061 aagcaagaat ggctataact gatgcgccga aaaaagacgc tccctcaccc aggatcgaga
     3121 ctgcgccatc cgtaccaggc cttgcttttt tgaaggtcgt tatcattctt ggctcacctt
     3181 tataagtctc acctatctca cttgccaggg tatctccgca tgctgttgcc actgaaccga
     3241 gatatgctgc catcaggacc tgcccatggg atggaaaaat cccgtatgcg acggccagtg
     3301 tgagggcagc cagtgagttg ctgaaaacat tcttatatcc gcgaactcct ccttttccct
     3361 gggcgatccc atgggccatc ttaaaatcgt atttgtaacg ggtaaacatg ctaccaagca
     3421 agaaaaaagc aagaaggata aaaaaccact taatatcgct tgatattatt ataactaccc
     3481 caagcaatgt tgcagaaagc atggcagata cgtcggcaac gcgcgccctg tatgaaagat
     3541 aacccagcac aaaagaaaat aaaagcgcgt tcattagata tgaagtatcc acattgtatc
     3601 cgaacagata gaaaagccac ataaccattg ccgagccaaa aggaatcact atattatctt
     3661 catcctttgt catggattca agaagagccc ccgtaagggc cccaataacg gaaaggaata
     3721 ttagcatccc cacaggaaca tacagggtga ggcctgatat ccatgctcct gagataaaag
     3781 caaaaacact ccctgaaatt agaaaaacca tactcgattt tgcggaataa actttatgac
     3841 ggctttgctt atcatttggg ttttgttttt tttctttatc aataatgtgt atgatatccg
     3901 caatgccgtc gccaaatgtc gtaatagcaa aagcagcccc tataatgaat agcgggaatt
     3961 tatacactcc tgcaatatct actccaacta taaggctgat cataagaaga atagcagtag
     4021 tcatgaatag ctgaatcatt ccaataagct tcctcgctct tgtatccgat tccctcgcaa
     4081 agaagcggaa aaatgatgaa tccggggaca cgaaataaag aagaatcgaa gttataagaa
     4141 ataaaagggc caatatgaaa tggtttgctt tgaaaaaagg aaaaaataga acaaggaatc
     4201 caagaagaat ataaatgctc tgtttctcat attcctttat cggccacatt aattctctga
     4261 gatggtattt acaggcaaat actttatcct tatactccat ttagagggag atgggattta
     4321 tctcaaaatt atacgaatgg aacctcagga caaggataag aacaatgaat gtgccgcatc
     4381 atattgtcct tgcgctttct gaaagtgatc tattttccga tacgaattat cttaaattac
     4441 attcctttgc ttgctggtgc aaagatatgg ggattaagac agttaccgtt tatataagta
     4501 tgatcgaagt agatacgcac ataagcaaca aaatctgcag taaattatca gcccagatac
     4561 cgcctgcgct caaggacatt acttcaaata ttaatattat taaaagaggg gacaaagaaa
     4621 tcagaaaaca cattacagat gatatggttc ttgatatctc acttggttat agtggaaagt
     4681 atgagttgac aagggcatta aaggaaatca tgtcccaggt aaaagcggga accataccac
     4741 ctgacgctat aaatgaagat gtgatcgaat cacacttatt attcaaatca gaacctgata
     4801 tcgttatccg ggcaggagga aaaagattga cggattttct catatggcag tcagtttatt
     4861 ccgagctcta ttttacagac gtcaactggc tggatttcag gaaggttgat ttcttaagag
     4921 tattaaggga tttccagaga cgtcaaagaa gattcggtaa gtaagatccg gtgaataaga
     4981 aaactaccat tttatcatgt tgttcaatac aacattgcga tttgcaagtt catggaggat
     5041 ctttactact tcttctttcg ggattgttat agatctcctt gatattcgga tcacatcttc
     5101 cacttccacc ttaccgtaat agctcaaaag cttcacgaca cgcataattg tattcttttg
     5161 tctctcatgt tctcgtgtct ggaatgttcg aattgcgcga agaaggtcaa ttcttcgaaa
     5221 ctctggccag aaaggggcac aaaaatacgc agcgcactca ttaccgtttg cctgccaggg
     5281 aaggaaattg gatatccttt catccccacc cgtccttatt ataagatcca catatgggac
     5341 cgaaccgctt gaaggatata aattatcaga tattgtctct tcgttaattt catatagtga
     5401 caattctccc gttttgactt tccaggccat cttctgtgca gcgtccacaa gctcctgtct
     5461 ccccccataa gcaagagcca cattaagata tacgccatcg taattccttg ttagatcctc
     5521 ggcatgtttt gctgataatt tgagcgctgg cgggagcagg tctatattcc cgatcaccct
     5581 taccctcata cggcgttggt gtgtgcgctc atcctggcat attttatcaa acttgatccc
     5641 gataagttca aaaagcctgt ctttctcgac atcattcctg tcgaaattct ctgtcgaaaa
     5701 ggcgtagact gtaagctgct tgatcccgat gtcaaaactc cagtcaagca ccttttctgt
     5761 tgtgtctgct cctttaaaat gaccataata ttgtgccagg ccgcgccttc ttgcgaaccg
     5821 cctgttaccg tccattataa tagcaacgtg cgccgggact ggtttgtcct ttatttcatt
     5881 ggcaagcaat gcctcataac tactatacaa caatccagtt aaactcaata tatcactcga
     5941 gtcacttaca gtatgagaag tatttaaatt tacccgaaat cttgcagttt gcgctgttcc
     6001 tccagttttt cttctgccgg tggagttgtt ccaatctgct ttaagacttt tatcgccact
     6061 tttgtgccaa ttatctttgc aactttggcc gcctgtgcaa cacggagtgc ctcgagatct
     6121 cttattccgg aatcaaacaa cttgcgcgca cgtacccgcc ctatacccct tatctgtata
     6181 aggtcaagaa gctctggact tgccccgtac tcaacacgtt tttcaagttc tcttgcttta
     6241 cctgcagatc gtcgtttttg aaattctccc agttgcgcca ttgaatggac aagccaaagc
     6301 gtggtttccg aaagccccct gatatctcct tccccaatac cgaatttctt cactatatct
     6361 tcttctgttt tctcatttat ccagtcaagc ataacacatg cggtcttgac ctgtgagagg
     6421 aaccattcgt aatcaacttt gaattggggc ggtatatgca agaattcagt atggtggtcc
     6481 ataataaggt cacccagcca attgtaatca tttgagcgca gatacagttg gcgcatatcg
     6541 ggtgttgttg ctaacagatg aagaagagtg agttcagtat aatctattcc tttctcatcg
     6601 attttcttca tcccttcaat gatcaatgat gctgaaagcg gatcgatata caggcgagag
     6661 accatttcac ccagccgggt ggcaaaaagt ccttcttttt ggattatcat ctccttttca
     6721 acgaggaaac ttatgatatt atcgatcacg atcttgaggc tccatggttc ctgctgggtt
     6781 gaataaaaag tggcgcgtat gaattccatc aattcattca tattccttgc atatccggtg
     6841 acgattatgg aaaggatgtg tgtgcgcaat gcgttttccg agccgagttt cgaccatata
     6901 ttttcagggc cggcaagtac atattgtttc atgagttcat ccagttcatc atatgttttt
     6961 gcgacaagga cagattcacc atatggatca agtcccggcc ttcctgctct tcctgccatc
     7021 tgcttgtatt caagaacagg tataggcacc tggccaaaat tcacatcata acgcctgtat
     7081 ccttttatta ttacccgccg cgccggcagg ttaagccccg ctgcaagagt aggggtcgat
     7141 gatattactt tgataatccc gttacggaat cccccctcta tgagcttgcg ttgctcggaa
     7201 ataagacctg catgatgaaa agcgcttcca ttttcaacac acattgcaag tttttttgct
     7261 gtatctactt cacttgtttc acgaacttcg gctgcaagaa catgtaattt ttcctttgca
     7321 tcatgatcca gcaggttcga tattgacatg ccaaggcgcc ccgccatacc ttcggagttc
     7381 ttccggctgc tttcaaaaac aaggcactgt ccaccctctt tgattgtatc ggctacaagc
     7441 gatatggcgc tgtccttatg gaattccctg atatggcgct ggctcctgtc gccaaaagtg
     7501 acggcgttcc cgaaaaagac tccttctttt aatccgatgg gacgccattc gctcacaaca
     7561 agttctgctg taagccattt tgccacttcc catgcattgc cgatagtcgc cgaaagcgct
     7621 attatttgcg gtttttgttt cattaatttt gcaagcacta tttcaagtgt tggtccgcga
     7681 tcaggcgaat caatgagatg gatctcatct gctatcacta ccgttatctc ttccatccag
     7741 tgcgacctgt tccgaagaag cgaatccacc ttttcggagg tggcgacgat gatatcaaaa
     7801 tttcccagcc actcgtcgcg ggaatcaaaa tcaccggttg cgatcccggt cgatactgcg
     7861 cgcccgtcgc ttttcttaat tgcctcaaag cccaggaaac gatcatattt ttcactggca
     7921 agagcgcgca gaggtacgat atagagtgct tttccttttt tattttcatt caataccgat
     7981 tgcagcataa caaattctgc aaggagggtt ttgcctgatg ccgtgggtat agcggcaacg
     8041 atattcttgc cttcaagaag cc
//
LOCUS       DATGBN010000307         1898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_334812, whole genome shotgun sequence.
ACCESSION   DATGBN010000307 DATGBN010000000
VERSION     DATGBN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334812"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VKL21_07560"
     CDS             complement(<1..1719)
                     /locus_tag="VKL21_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HMB45662.1"
                     /translation="MTDLNDSSQSFKYPPNLTIVGLGGCGKKLARKICNYDWLLRYYS
                     RDMNKLKIYTIDTDANEYIDDSSWEKKIMEKVDSLEGAGNIEFKSYFLPRLANISHVS
                     DLASADVSGSIKRFRSIKSWWLHDPENSGITFQDLKRIDPFIMDDFGGGVHRRRAVSK
                     AILYKVLSEGQANGFPTFSTPGATAIIVGLGGGTGSGMFIDLARYIKEKRDTAIYLFA
                     VLPTTKEGEKEQLNAAVSLTELEYLNVSHEERLFDHVIFTSLGPTEYTSGLHELEAVE
                     EFDSVFPQIITNFFHIERSDLNLSDARKSYSSFIFANSHVIEYPVEELKELKKQYSQI
                     IQKLEEINNDRKSINAAIESFLVKFNINGDATPTLEIFEFIKTEYRNIEKVWTNDIAK
                     LLNYHSVEQIEEFIKYNISEIQFEKIGSYNELLSYISRVRNFEQSVAQDKLKDEIDKK
                     LFRLIPEALDTLEKNARLFKRVATVENEECRSVMIDILKGKKDISPLQGALNAKNQEI
                     QTLNDKLKLEMDKIATLGFIPSDIEKKVEDKLKDIDFELEYYAKISRNLKYLPGREQK
                     LKEMLDQ"
     gene            complement(1716..>1898)
                     /locus_tag="VKL21_07565"
     CDS             complement(1716..>1898)
                     /locus_tag="VKL21_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45663.1"
                     /translation="VNESNLLADHLTENNEWPLYYWTIPGIIIGFLIGGLIGLRIGYK
                     AKIGNPDETDVGEDKK"
BASE COUNT          527 a          402 c          311 g          658 t
ORIGIN      
        1 ttgatccaac atttctttca gtttttgctc tctacctggg aggtacttca aattcctact
       61 aatttttgca tagtactcaa gctcaaaatc tatgtctttt aatttatctt cgaccttttt
      121 ctctatgtca ctgggtataa aacctagcgt ggctatttta tccatttcca gcttcaactt
      181 atcatttaga gtttgaatct cctgattctt ggcgttcagt gctccttgaa gaggagagat
      241 atcctttttt cctttcagga tatctatcat tacagacctg cattcttcat tttcaacagt
      301 cgcaactcgc ttgaacaaac gtgcgttttt ttccagagtg tcgagggctt ccggaattaa
      361 gcggaacagc ttcttatcaa tctcatcttt gagtttatct tgtgcaacgc tttgttcaaa
      421 attacgaacc cgtgaaatat aggatagaag ttcattataa cttccaatct tttcaaattg
      481 aatttctgaa atgttatatt taataaactc ctctatttgt tcaacactgt gataattaag
      541 gagctttgcg atgtcgtttg tccatacttt ttcaatattt ctatactccg tttttataaa
      601 ctcgaagatt tccagtgttg gagtggcgtc tccatttata ttgaacttaa caaggaaact
      661 ttcaattgct gcgtttatac ttttcctatc attattgatc tcttcaagct tttgaatgat
      721 ctggctgtac tgcttcttta actccttgag ctcttctaca gggtattcga ttacatgcga
      781 attggcaaag ataaaagaag aataggattt tcttgcgtca ctaaggttga gatcacttct
      841 ctcaatgtgg aagaagttag taataatctg cggaaaaaca gaatcaaatt cttcaactgc
      901 ttcaagctca tgcagtccgc ttgtgtattc agtagggcct aaagatgtaa atattacatg
      961 atcaaagagt ctctcctcat gtgatacgtt taagtattca agctccgtaa gagaaacggc
     1021 agcattaagt tgttcttttt ctccctcttt tgttgttgga agcactgcaa aaagatagat
     1081 tgcagtatct cttttttcct tgatatatct tgcaaggtct atgaacattc cggagccagt
     1141 acccccaccg agcccaacaa tgatggctgt ggctcctgga gtcgaaaagg ttgggaaccc
     1201 atttgcttgc ccttcactca gaactttata tagaatagcc tttgaaaccg ctcttcgacg
     1261 atgcacaccg cccccaaaat catccataat gaagggatct atacgtttca aatcctggaa
     1321 agtgattcca ctattttcgg gatcatgcaa ccaccatgat ttgatagatc ggaacctttt
     1381 tatacttcct gaaacatcag cgctggctaa atcggacacg tgtgatatat ttgcaagtct
     1441 tggtaggaaa taggatttaa attcgatatt tcccgctcct tccaggctat ctactttctc
     1501 catgattttt ttttcccagc tactatcatc aatatattca ttagcgtcag tatcaatagt
     1561 ataaatctta agtttgttca tatcacggga atagtagcgt aataaccaat cataattaca
     1621 gattttacga gcaagttttt tcccacatcc ccccaaaccc acaattgtca ggtttggagg
     1681 atacttaaaa gattgacttg agtcatttaa atctgtcatt ttttatcttc tccaacatct
     1741 gtttcatcag gattacctat tttcgcttta tacccaatcc tcaaaccaat taaaccacca
     1801 atcagaaaac ctataattat gcctggaata gtccaatagt atagcggcca ttcattattt
     1861 tcagtcaaat ggtctgcaag caagtttgac tcgttaac
//
LOCUS       DATGBN010000308         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_528872, whole genome shotgun sequence.
ACCESSION   DATGBN010000308 DATGBN010000000
VERSION     DATGBN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528872"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VKL21_07570"
     CDS             complement(<1..408)
                     /locus_tag="VKL21_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMB45664.1"
                     /translation="MNVVIEKAQEKDRPFIFELLEQANMHYIPSKEMPELAYENYFVA
                     KLNGKIVGFCGYKILSATEAKTELMVVDNKFRGKGIGNMLQIRRMEDMLKKDIKTLVT
                     NADLPATIEWYKKHFGYEEVGKLKKYHEFGNPNI"
     gene            complement(422..2281)
                     /locus_tag="VKL21_07575"
     CDS             complement(422..2281)
                     /locus_tag="VKL21_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HMB45665.1"
                     /translation="MPGLVGIIISKNENKSADKLLSKMCQSIKHETWYKIDTHISKTA
                     GLGRVHLGIFNPEPQPIFNEDKTLCILMEGEVYDYQDLKKELILKGHKFSIENDPEFI
                     LHLFEEYGEQFIHKLNGSFTLVIWNEKSQKLMIFNDRYGLRPLYYTLHNECLLFGSEI
                     KPILQDETFERIVDDKSVAEFFSFGYILGNKTLFQEIKLIPPASMITYEKGQISIEKY
                     WDFNFDEEYEDHPEEYYVKESSRLIIQAVERQMKGNHRIGVPLSGGFDSRTIVASISK
                     KHYPIHTFTFGKRNCYDVEFSRMIANKLGTVHHFFEFEPDDLVSYAEKAVYLTDGMKN
                     CVHAHRMQTYNEMRQYIDVTLSGWIGDSTIGRARDVFNVIEDNFELFKGMVFHQPPDD
                     LLGNLFKDDYFQLIGKNLEISKNHVIQMGKDSDIKYSSNRFMYYNLKERQRRFSLMGH
                     ILARSSLEVRTPFSDYDFIDFNLKIPPKFKVRNRLYKKVILTMFPHLSNVPTQKTGLP
                     ISLSIFQERTYNLLQTVKRIMNMIYKKTFRSELFANPNDFASYDDWMRHNKKLREYIF
                     SILVDERISKRPYFNQKYIKEILDIHMSGKKNYSELIGRLMTFELWNRLFIDK"
     gene            complement(2562..2876)
                     /locus_tag="VKL21_07580"
     CDS             complement(2562..2876)
                     /locus_tag="VKL21_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45666.1"
                     /translation="MRTLSDIDKKILAKLAPELEGATGPAPGHDYKFILLPVSHKLSD
                     SPEDFANRANRLTDEELVYLIDLILHGDEDVRSLAEGDYDALVEMIEKRLPEKAKDLK
                     NL"
     gene            complement(2896..3270)
                     /locus_tag="VKL21_07585"
     CDS             complement(2896..3270)
                     /locus_tag="VKL21_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMB45667.1"
                     /translation="MNKKVNVIVTQPPYGKEDAFSAVPLAASQVAAGNEASMIFVSGG
                     VHSIVKGQKTGYGDLAVWFKDVPSVEAEIIKNIDLVSFFALDRDMAKRNIADTEIITG
                     IKKITLPELTDMILEADVNLVF"
     gene            complement(3276..3644)
                     /locus_tag="VKL21_07590"
     CDS             complement(3276..3644)
                     /locus_tag="VKL21_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMB45668.1"
                     /translation="MTGKQLTIVSINGPYRDEGVFTMIRLAHAAKEKGINVNFFNYLD
                     ATLIAHKEQAPKEFPPIETIFGTIVKKGIKKPKADAIACIKCTDARGVTKQQTEGVII
                     GGLYDLGEWTGESDKTILIG"
     gene            complement(3653..3976)
                     /locus_tag="VKL21_07595"
     CDS             complement(3653..3976)
                     /locus_tag="VKL21_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMB45669.1"
                     /translation="MTNLLLVLSKDPYTTETPDLVLDIGMNAKEKGNDVSLYLIEDGI
                     TAARKSEFGNKLAAAEKKGIRIFADDKAVLSRSLTDKLIGGVEIKEIGTLLDYIMEYD
                     RVVWF"
     gene            4100..4184
                     /locus_tag="VKL21_07600"
     tRNA            4100..4184
                     /locus_tag="VKL21_07600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4134..4136,aa:Ser,seq:tga)
     gene            complement(4254..>4658)
                     /locus_tag="VKL21_07605"
     CDS             complement(4254..>4658)
                     /locus_tag="VKL21_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HMB45670.1"
                     /translation="ILVGETLFLYISPSLIKILAGLLFIVFGIWILLSQRTSCDAEEN
                     KSYSNPLISTFSMITLAEMGDKTQLSAITLAAKFDSPYLVFLGAIFALGLITLIGILV
                     GEKLCKIIPLSKIRQGAGVVFILFGILFLAGF"
BASE COUNT         1341 a          976 c          752 g         1589 t
ORIGIN      
        1 tatattggga tttccgaact catgatactt ttttaactta cccacttcct catacccaaa
       61 atgtttttta taccactcga tcgtagcagg taaatcagca ttggttacaa gcgttttaat
      121 atctttcttt aacatatctt ccattctgcg aatttgaagc atattcccaa tgccttttcc
      181 gcgaaactta ttatccacta ccataagctc tgttttagct tccgttgctg aaaggatctt
      241 atatccgcaa aacccgacta tcttaccatt tagtttagca acaaaataat tctcatatgc
      301 taattccggc atctcttttg atgggatata atgcatattg gcctgctcta atagttcaaa
      361 gataaagggt ctgtcttttt cctgtgcttt ttcaataacc acattcatat ttgctccgta
      421 cttatttatc tataaataat ctgttccaca attcaaaagt cattaaccga cctattaatt
      481 ccgaataatt ttttttgccg ctcatatgaa tatccaggat ttcttttata tatttttgat
      541 tgaaataagg ccttttggat attctttcat ccaccaggat actgaatatg tattctctta
      601 attttttatt atgtctcatc cagtcatcat aactggcaaa atcattagga ttggcaaata
      661 attcggatct aaacgttttt ttatagatca tattcataat tcttttaacg gtttgtaata
      721 aattatatgt tcgttcttga aatattgaga gagatatggg cagtcctgtt ttctgggtgg
      781 ggacatttga caaatgcgga aacatcgtta atatcacttt tttataaaga cgatttctaa
      841 ccttaaattt gggtggaatc tttagattaa agtctataaa atcataatct gaaaaaggcg
      901 ttctgacttc taatgagctt ctggctaaaa tatgtcccat taatgagaat cgacgctgcc
      961 tttctttaag attatagtac ataaatctat tactcgaata tttaatatca gaatccttac
     1021 ccatttgtat aacatggttt ttggaaattt ccagattttt cccaattaat tgaaagtagt
     1081 catctttaaa taaattgcca agtaaatcgt ctggcggctg atggaagacc atgcctttaa
     1141 aaagttcaaa attatcttcg ataacattaa aaacatccct ggctcgcccg attgttgaat
     1201 cgccaatcca accactgaga gtaacatcaa tatattgtct catctcatta tacgtttgca
     1261 ttctatgtgc atgaacacag ttcttcatac cgtcagttaa ataaactgct ttttctgcgt
     1321 aagagactaa atcatccggt tcaaattcaa aaaaatggtg gactgtcccc aatttatttg
     1381 ctatcatccg agaaaattca acatcataac aatttctttt accaaatgtg aaagtatgta
     1441 tcggataatg tttcttgctg atcgaagcga ctattgtcct tgaatcaaat ccaccgctta
     1501 atggaacccc tatcctgtga ttccctttca tttgccgctc cacggcctgt attataagtc
     1561 ttgatgattc tttaacataa tactcctccg gatgatcttc atattcctca tcgaaattga
     1621 aatcccagta tttctctatt gaaatttgac ccttctcata agtgatcata gatgcaggag
     1681 gaataagttt gatttcctga aaaagcgttt tgttacctaa gatatatccg aaagaaaaaa
     1741 attccgcaac tgacttatca tcaactatcc tttcaaaagt ttcatcctgg agaattggtt
     1801 taatttcaga tccaaataat aagcattcat tatgcaaagt ataatataat ggccttaaac
     1861 cgtagcgatc attgaagatc atcaatttct gtgatttctc attccagata actaatgtga
     1921 atgatccatt caatttgtgt atgaattgct caccatattc ttcaaataaa tgcagaataa
     1981 attccggatc attttcaatg gaaaatttat gccctttaag tataagttct ttttttaaat
     2041 cctgataatc gtagacttct ccttccatca ggatgcataa agtcttatct tcgttaaata
     2101 ttggctgcgg ttccggattg aagattccca ggtgtactct tcccaatcca gcagttttgc
     2161 ttatatgagt atcaatctta taccatgttt cgtgcttaat tgactgacac atttttgaca
     2221 gtaacttatc agcactttta ttttcattct tggatattat tatcccaacc aatcctggca
     2281 ttttagtttc atcctttctt tattagtact aatttttgat tatttcgaaa gaggatattt
     2341 gcaaatgact aaatattcga ctctaaaaaa tatatgtata tatattttat tattatttat
     2401 attataaatt tggcacagtc acataatcca gttatgaata atatccccat catttaaaaa
     2461 tctatctaat gtcccaaata taaccaacac ttttgggatg gtttacctac ttgcgattgt
     2521 aatatttaat ccctatctat tccctgaaaa gagttgtcga ttcataggtt ttttaggtct
     2581 ttagcctttt ccggcagcct tttctcgatc atctcaacaa gcgcatcata atccccttct
     2641 gcaagtgagc gcacatcctc gtcgccatgc aaaataagat ctatcaggta aacaagctct
     2701 tcatccgtca acctgttagc cctgttcgca aagtcctccg gggagtctga taatttatgt
     2761 gagaccggaa gcaggatgaa cttgtagtca tgccctggcg cggggcctgt tgcgccttca
     2821 agttcaggcg caagttttgc aaggatcttt ttatcaatat ctgagagcgt tctcatgtgg
     2881 aagaaacata tttcgtcaaa ataccagatt cacatccgct tcaagtatca tgtcagttaa
     2941 ttcgggaagt gttatctttt ttatcccggt aataatctca gtatcggcaa tgtttcgttt
     3001 tgccatatcc ctgtcaagtg caaagaagct cacaaggtct atgttcttga tgatttccgc
     3061 ttccactgac gggacatcct taaaccatac cgcaaggtcc ccgtatcctg ttttttgtcc
     3121 tttcactatt gaatggacac ccccgcttac gaagatcatt gaagcttcgt tccctgccgc
     3181 aacctgcgaa gcagccagcg ggaccgccga aaatgcatct tcttttccat aaggaggttg
     3241 tgttacgatt acatttactt tcttgttcat tttgctcacc cgatgagtat tgttttatcc
     3301 gattctccag tccattcgcc aaggtcatat agtccgccga ttatgacgcc ttctgtctgc
     3361 tgttttgtga cacccctggc atcagtgcat tttatgcatg caatggcatc agctttgggt
     3421 ttctttatgc cctttttcac tattgtcccg aaaatggtct cgatcggcgg gaactctttg
     3481 ggcgcctgct ccttgtgagc gatcaatgtg gcatcaaggt agttgaagaa atttacatta
     3541 atcccttttt cttttgccgc atgggcaagc ctgatcattg tgaatacacc ttcatccctg
     3601 tagggaccgt ttattgaaac tatcgtaagc tgttttcctg tcatattatc acctaaaacc
     3661 acacgactct atcatattcc attatataat caagaagtgt tccgatttct tttatttcaa
     3721 ccccgccaat taatttgtca gtgagcgacc ttgaaagtac cgctttatcg tctgcaaata
     3781 tcctgatgcc tttcttttcc gccgctgcaa gtttattccc gaactctgat tttcttgcag
     3841 cagttatgcc gtcttcgatg agatataatg aaacgtcgtt tcctttttcc tttgcgttca
     3901 ttccgatatc aagtacaagg tcgggcgttt ccgttgtgta tgggtccttt gaaaggacaa
     3961 gtaataaatt tgtcattttt tcctctgtaa gatcatttaa gtatagattc tattatatag
     4021 ccaataatta tcgaatgagt ttaaatgttt ccatgtaggc aatattaata aaccatgccg
     4081 accatttcgc aggaaatatg ccgaggtagc ccagcggcct aaggcgccgg tcttgaaaac
     4141 cggttgtgca ccgcacagcg ggagttcaaa tctccccctc ggcgctcaat aattagtaca
     4201 aatccgaata acccttttat gcagcccata taaatagaaa caggcaagag cgttcagaaa
     4261 ccagccaaaa acaatatccc aaagaggata aataccactc cagcaccctg tctgattttc
     4321 gataaaggta taattttgca caatttttcg ccaacaagta tgccgatcaa ggttattaat
     4381 ccaagcgcaa atatcgctcc aagaaacact aaataaggtg aatcaaattt tgctgccaaa
     4441 gtaatagcag aaagttgtgt tttatctccc atttcagcaa gagttatcat cgaaaatgtt
     4501 gagatcaatg gattcgaata tgatttattt tcttctgcat cacaacttgt tctttgtgat
     4561 aataatatcc agatcccaaa aacaatgaac aataaacctg ctaaaatctt gatcaaggaa
     4621 gggctaatgt acaggaatag ggtctcacct acaagtat
//
LOCUS       DATGBN010000309         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_23696, whole genome shotgun sequence.
ACCESSION   DATGBN010000309 DATGBN010000000
VERSION     DATGBN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23696"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="gcvT"
                     /locus_tag="VKL21_07610"
     CDS             complement(<1..910)
                     /gene="gcvT"
                     /locus_tag="VKL21_07610"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMB45671.1"
                     /translation="MGKIALFRTPLYQTHIHLGARMMGFHGWEMPVEYSGTMKEHKAV
                     RGSAGLFDVSHMNKLIIKGPGSFEFLQFISTNDISELNVNGMKYSIICRDDGTIVDDI
                     VMIRKEDHFFLVTNTSRKDILMNWLDKHPEQGVKIEDVTSDMATLALQGPDSDRIISG
                     ISDNIDKIRFWCGGKRQVAGIDTYITRSGYTGEDGFEIYCRSQDASQLWESIMDAGDI
                     KPAGLGARDTLRLEMGYILSGMEITDENNPLEAGLEWAVKWKKDFIGKEALLKIKKEG
                     VKRKLAGVKLDRGIPRHGYDIVGSSSE"
     gene            1057..>2266
                     /gene="gcvPB"
                     /locus_tag="VKL21_07615"
     CDS             1057..>2266
                     /gene="gcvPB"
                     /locus_tag="VKL21_07615"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HMB45672.1"
                     /translation="MSNNNHILENKRSCGIENFDFRLIFEKSKKNTFKKCQQLPKNIT
                     RDTLDIPELPEPEIIRHYTNLSKINFGVDTGMYPLGSCTMKYNPRFTEHLAAHEQNNV
                     HPYQDESTIQGSLEIMYLLKKYLTNICAMDDFSLQPAAGAQGEFTAMAIIKAYFERKN
                     DLRTEVIVPDSAHGTNPKSAAMAGFDVVEIQSDEKGYIDIEILKSAVSEKTAALMLTT
                     PSTLGFYEKNIMKIEQIIHGAGGLLFYDGANLNGIIGKIRPGDMGFDCMSLNLHKTFA
                     VPHGGGGPGCGPIGVKKHLIDFLPVPAIEYDGQKYYLDHDRPDTIGKVHGFNGNYLSV
                     LKALGYIMRQGSAGLKRISEFSVLNSNYMVSRLSNAYDIKNPDIHRKHEAVFSAARQK
                     KNDITALDIAK"
BASE COUNT          662 a          502 c          439 g          663 t
ORIGIN      
        1 tttcactgct gcttcccact atatcataac catgtcttgg aatccctcga tcaagtttta
       61 ctcctgcaag tttccttttt actccttcct tttttatctt taacagggcc tcctttccta
      121 tgaaatcttt tttccatttc acagcccatt caagtccggc ctcaagagga ttattctcat
      181 ctgttatttc cattcctgaa aggatgtaac ccatttcaag cctcagcgtg tcgcgtgcgc
      241 caaggcctgc gggtttaata tcccctgcat ccattatcga ttcccagagc tgcgaagcat
      301 cttgcgatct gcaatatatc tcaaaaccgt cttcacctgt atatcctgat cgtgtaatgt
      361 aggtatcgat ccctgcaacc tgcctttttc ctccgcacca gaacctgatc ttatctatgt
      421 tatccgaaat tccggaaatt atcctgtctg aatcaggtcc ctgtagtgca agagttgcca
      481 tatcggaagt aacatcctct atttttacac cttgctccgg atgcttatca agccagttca
      541 taaggatatc ttttcgagaa gtattcgtta cgaggaaaaa atggtcttcc tttctgatca
      601 taacgatatc atcgacaatg gtaccatcat ccctgcatat gattgaatat ttcatgccgt
      661 tgacattgag ttctgatatg tcgttagtag atatgaactg gaggaactcg aaagatcccg
      721 gacctttgat tattaattta ttcatgtggg atacatcaaa aagcccggct gatccccgga
      781 cagctttatg ttctttcatt gttcctgaat attccactgg catctcccat ccatgaaatc
      841 ccatcatcct ggccccaaga tgaatgtgcg tttggtaaag cggtgtccga aatagtgcta
      901 ttttccccat ggtacttaat tcagtgttaa tcctttatta tgattttgca atgtatttta
      961 attttggaat gaaggaaaac tatttttctc catagcccat aattatctaa taattatcaa
     1021 atttggagtt ggcaaattca gttgcagggg atcatcatgt ctaataataa tcacattctt
     1081 gagaataaaa gatcatgtgg aatcgaaaat ttcgatttcc gtttgatctt tgagaaatca
     1141 aagaaaaaca cattcaaaaa atgccagcaa ctgcccaaaa atatcacacg cgataccctt
     1201 gatatccccg aacttccaga acctgaaata atccgccatt acacaaacct ctcaaaaatc
     1261 aatttcggtg ttgatactgg catgtacccg ctgggttcat gcacaatgaa atataatccc
     1321 aggttcacag aacacctagc agctcatgaa caaaacaatg tacatccgta ccaggacgaa
     1381 tccacgatac agggttccct ggaaataatg tacctgttga aaaaatatct gacaaatatt
     1441 tgcgcaatgg atgatttctc gcttcaacct gcagcaggag cccagggcga attcactgca
     1501 atggccatta taaaagccta ttttgaaaga aaaaatgatt tgagaactga agttatcgtg
     1561 cctgattccg cccacggcac aaatcccaaa agtgcggcca tggcgggttt tgatgtggtt
     1621 gaaatacaat ctgatgaaaa aggttacata gacattgaaa tactcaagtc ggcagtttct
     1681 gaaaagacag cagctttaat gttgacgact ccgagtactc tgggtttcta tgaaaaaaat
     1741 atcatgaaaa tagaacagat catccacggc gctggagggc ttttatttta cgacggcgcc
     1801 aacctgaacg ggataatcgg aaaaattcgc cccggagata tgggatttga ctgtatgagc
     1861 ctgaatctcc ataagacttt tgcggtacca cacggcggcg gcgggcctgg atgcggacca
     1921 atcggggtca agaaacatct tatcgatttt ttacctgttc ctgctatcga atatgatggg
     1981 cagaaatatt atcttgatca tgacaggccg gatacaatcg ggaaagtgca tgggtttaac
     2041 ggtaactatc tatccgtact caaagcgctg ggatacataa tgagacaggg gtcagctggt
     2101 ttgaagcgta tctcggagtt ttcggtattg aattccaatt atatggtatc aagactttca
     2161 aacgcttatg atatcaagaa tcctgatatc cataggaagc atgaagcagt gttcagtgca
     2221 gccaggcaga aaaaaaatga tattactgca ttggatatcg caaaac
//
LOCUS       DATGBN010000310         5381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_257467, whole genome shotgun sequence.
ACCESSION   DATGBN010000310 DATGBN010000000
VERSION     DATGBN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257467"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VKL21_07620"
     CDS             <1..714
                     /locus_tag="VKL21_07620"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMB45673.1"
                     /translation="REIDFKRFREIADEAGALLLSDIAHIAGLVVAGVHCDPVPYSDF
                     VTTTTHKTLRGPRGGMIMCRQEYAKDIDKTVFPGIQGGPLMHVIAAKAVAFKEAMSRE
                     FNDYQKQIVKNAKKIAGELMIRGNELISGGTDNHLMLVDLTPLGITGKDAEAKLGEAG
                     IILNKNTIPFETKSPFITSGIRIGTPAATTRGMKEKEMIIIADAIDEVIRNSNEANKI
                     KEVKRTILDLCNQFPIPYE"
     gene            723..1598
                     /locus_tag="VKL21_07625"
     CDS             723..1598
                     /locus_tag="VKL21_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HMB45674.1"
                     /translation="MEPLPEVTDPRVIDGRKIAQDIEADVKRDVEIFVNEHGFKPALA
                     TILVGEHPPSKLYVKLKHWACKRVGIVSEDHKYSQETKQDEIIELIGTLNMRPEIHGI
                     LVQLPLPKHIDERAVMMQIAPEKDVDGFNPLNMGKMLIGKEGFVPCTPKGIIRALSEF
                     NIDPKGMEVVVVGHSNVVGKPAAMMLLNRNATVKICHVFSKDLKSQTQKADILIVATG
                     VRGLIKADMVKEGAVVFDVGITWQDEKVYGDVDFEDVLPKVKLISPVPGGVGPMTIAI
                     LMEHTLQAAKIQNKK"
     gene            complement(1604..2833)
                     /locus_tag="VKL21_07630"
     CDS             complement(1604..2833)
                     /locus_tag="VKL21_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGF-pre-PGF domain-containing protein"
                     /protein_id="HMB45675.1"
                     /translation="MNRYGKISKLVFAVLFTRIRDVKDMYSKLYSNLQKLNSATTMHL
                     MYKVFLGSIVVLITALPIILNASALIENPVIWITSLKEDDVISTSPIEVIGNFSGYVN
                     NVEVRVGTGSWLLVAGAPTGTLLITGNSTWTYSIQLTAGSNKITAKATDISNNSAETS
                     VNVTYSPSVNPNRDSSGSGSGGGGGSGGSGENFSNIDVIEKYDQAIYKDVMTSYKFIN
                     PKNPIIFVNITGNVNYDGITTMVEALKDTSTIVKSAAMGNVYMNVNIWVGTSGFATPK
                     NIKEAVIRFRVENSWIGDLSNGDIKLLRWDGSKWVTLETSEISKDSTFTYFEANTDSF
                     SPFAITSLKEKSALNGVMFSQEKTSIEKAKKSDGNKAKGNQPESNKPDVLMNWFIISG
                     IFVLIGLIIEVLRIKKK"
     gene            3051..3329
                     /locus_tag="VKL21_07635"
     CDS             3051..3329
                     /locus_tag="VKL21_07635"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HMB45676.1"
                     /translation="MKKEQIFITVIGKDQKGIIARISNAVFAHNINIEDLNQKIMGGY
                     FVMTMLADIADSPITITRLKKELSAIEAEMDLKIQVQHENIFKTMHRV"
     gene            3333..4691
                     /locus_tag="VKL21_07640"
     CDS             3333..4691
                     /locus_tag="VKL21_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HMB45677.1"
                     /translation="MEYSLEEVMETVRMTLYHNFDIRTVTLGINLKDCINSDLDIFKD
                     NVYDRVSGYGRRLILEAQKLEDKYGIPIINKRISVTPVSLIIEACAEDDAPLEIAKTL
                     DKASKDVGIDFIGGYGALVQKGMTRADKRLIDSLPEVFSCTERVCGFLNVASTVSGMN
                     MDAIIMLGKTLKEIAGRTENGIGCAKFVIFANAPEDNPFMAGAFHGVGEPDFSLNIGI
                     SGPGVVRSVVEKNRDCDLTELSGVIKRTTFKITRAGELIGRELAHNLSVPFGIVDISL
                     ASTTKVGDSVAGIVEMMGVEKMGAPGSTAALALLVDAVKKGGAMASGNIGGLSGTFIP
                     VSEDSCMNEAVRVGALSIEKLEAFTAVCSVGLDMIAIPGDTKAQTISAIIADELAIGV
                     VNNKTTGVRLIPVPGKKAGDYVNFGGLLGETYVMNISAFSSDDFILRGGRMPGPVTSM
                     RN"
     gene            complement(5200..>5381)
                     /locus_tag="VKL21_07645"
     CDS             complement(5200..>5381)
                     /locus_tag="VKL21_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45678.1"
                     /translation="VDISAIAGGTNKTPIIKAKIIVDTGTKRAMPSVVKKDPKTNMNF
                     DLLSFVQKEIFFSNF"
BASE COUNT         1622 a         1032 c         1209 g         1518 t
ORIGIN      
        1 cgtgaaattg attttaaaag atttcgggaa attgctgatg aggcaggagc gcttttactt
       61 tcggatatag cgcatattgc aggattggtc gtggcgggag tgcactgtga ccctgttccc
      121 tattcggatt ttgtgacaac gacaacgcat aagacgttgc ggggaccgcg cggcggaatg
      181 ataatgtgca ggcaagaata cgcaaaagat atcgataaga cagttttccc cgggatccag
      241 ggaggtcctc tcatgcatgt tatagccgca aaagcagttg ccttcaaaga agccatgagc
      301 agggaattta acgattacca gaaacaaata gtgaagaatg caaaaaaaat cgcaggtgaa
      361 ctgatgatca ggggcaatga actcatatca ggcggaacgg ataaccacct gatgctggtt
      421 gatctgacgc cgcttggtat cacaggcaag gacgctgagg caaaacttgg tgaagccggg
      481 ataatcctga acaagaatac cattcctttt gaaacaaaaa gtcccttcat cacaagcggt
      541 atccgtatag gcacaccggc tgcgaccaca cggggaatga aggaaaaaga aatgataatt
      601 atcgcagatg ctatcgatga ggtcattcgg aacagtaacg aggcaaataa aatcaaggaa
      661 gttaagagaa ctattctcga cctgtgcaac cagttcccga tcccatacga gtgaggacac
      721 ctatggaacc gcttcccgag gttacagacc ccagagtcat agatgggaga aaaatcgcac
      781 aggacattga ggctgatgta aagagagatg tagaaatatt tgtaaatgag catggcttca
      841 agccggctct tgccacaatt ctagttgggg aacatcctcc atctaaactg tatgtaaaac
      901 ttaaacattg ggcatgcaaa cgtgttggca tcgtatctga agatcacaaa tattcccagg
      961 aaacaaaaca ggatgagata attgaactca tcgggacatt gaacatgcgt cccgagatac
     1021 acggcatact cgttcagttg cctttgccca aacacatcga tgagcgcgct gtgatgatgc
     1081 agatagcgcc tgaaaaagat gtggatggtt tcaatcccct caatatgggt aaaatgctca
     1141 tcggaaaaga gggttttgtg ccatgtactc ccaaagggat aataagggcg ctatccgaat
     1201 tcaatataga cccgaaagga atggaggtcg tagtggtcgg gcacagcaat gttgtgggca
     1261 aacccgctgc aatgatgctt ctgaaccgca acgcaacggt aaagatatgt catgtgttct
     1321 caaaggatct taaaagccag acacaaaaag ctgatatcct cattgtggcc accggtgtac
     1381 ggggattgat aaaagctgat atggtaaaag aaggcgccgt agttttcgat gtaggtataa
     1441 cctggcagga tgaaaaagtt tacggggatg tggattttga ggatgtgttg ccaaaggtga
     1501 aattgatatc ccctgtaccg ggaggcgtag gtcccatgac tatagctatt cttatggaac
     1561 atacactgca agcggccaaa attcagaata aaaaataaat attttatttc ttcttaatcc
     1621 tgagaacctc aatgatcaaa ccaatcaata cgaaaatacc gctgatgata aaccagttca
     1681 taagaacatc aggtttattg ctttcaggtt gatttccttt ggctttattt ccgtcagatt
     1741 tttttgcttt ttctatagaa gttttctctt gcgaaaacat gactccattc aatgcggatt
     1801 tttccttcaa agaggtaatt gcgaaaggcg aaaagctatc ggtattcgct tcaaaataag
     1861 tgaatgtact atctttcgat atttctgaag tttcaagagt tacccatttg cttccgtccc
     1921 atcttagcaa tttgatatcc ccatttgaaa ggtcaccgat ccatgagttc tctaccctga
     1981 accttatcac tgcttccttt atgttctttg gtgttgcaaa gcccgacgtt cccacccaga
     2041 tatttacatt catatataca ttgcccatag cagcggattt cacaattgta gaagtgtctt
     2101 tcagcgcttc caccatcgta gtaataccat cataattaac atttcctgtg atgttcacga
     2161 aaataatggg attttttggg tttatgaatt tgtaggaggt cattacatct ttgtagatgg
     2221 cctggtcata tttctcaatc acatcgatgt ttgaaaaatt ttcacctgat cctccgcttc
     2281 ctccaccgcc tccactacca ctaccactac tatctctgtt tggattgaca gatggagaat
     2341 atgtcacatt gactgaggtt tcggcggaat tattggaaat atccgtagct tttgccgtta
     2401 tcttgttcga tcctgctgta agttgtattg aatatgtcca tgtcgagttg ccagtgatca
     2461 gcaatgtacc tgtgggggcc ccagcgacta acagccatga acctgttcca actctcactt
     2521 ccacattgtt cacataacct gaaaaattcc cgatcacttc tatgggtgaa gtactgatta
     2581 catcatcttc tttcagtgat gtgatccata tgaccgggtt ttcaataaga gccgaagcat
     2641 tgagaataat tggcaaggcc gtgatcaaaa caactatact acccaaaaac accttataca
     2701 taaggtgcat ggtggtggct gaatttagtt tctgtaaatt ggaatataat tttgaataca
     2761 tatccttcac atccctgatc cgtgtaaaca acacggcaaa gaccaatttc gatatctttc
     2821 catatcgatt catgggcact gttgaatata ataccataaa tatataagct tatttactgt
     2881 ataattatat taaaaatgat tattgtcatt attagtatga tgttataata ctaatagcgg
     2941 aagatacatt tttcaataaa agatagtaga atctcaagat tgatagtgta tcaataatat
     3001 ttatgctact atgagaatgt gaatgtctgc caggatcaaa agaggttcca atgaaaaaag
     3061 aacagatatt tattaccgtg atcggaaaag accagaaagg aataatcgcg aggatttcaa
     3121 atgccgtttt tgctcataat atcaatatag aagaccttaa ccagaaaata atgggcggat
     3181 attttgtgat gaccatgctt gccgatatcg ccgattctcc aataaccata acccggttga
     3241 aaaaagagct ttcagcaatc gaggctgaga tggatcttaa gatccaggta cagcatgaga
     3301 acatcttcaa gaccatgcac agggtgtaat tcatggaata ttcacttgaa gaggtcatgg
     3361 aaactgtccg tatgaccctt tatcataatt ttgatatcag gaccgtgacc ttggggataa
     3421 accttaagga ttgtatcaat tcagaccttg atattttcaa ggataatgtg tatgacaggg
     3481 tgtccggata tgggcgaaga cttatccttg aagcgcaaaa actcgaagat aaatacggca
     3541 tcccaataat aaataaaagg atatcggtca ccccggtctc acttatcatc gaagcttgcg
     3601 ccgaggatga tgcccctctt gagatcgcaa agacccttga taaagcatca aaggatgtgg
     3661 gtattgattt catcggcggt tatggggcac ttgtccagaa aggtatgacg cgggcggata
     3721 aacgtctcat cgactcactc cctgaagtct tctcatgtac tgaaagagta tgcggtttcc
     3781 tgaatgttgc ttcgactgtt tcgggcatga acatggatgc tatcatcatg ctggggaaaa
     3841 ctttaaaaga aattgcaggc agaaccgaga acggcatcgg atgcgcaaag ttcgtgatct
     3901 ttgcaaatgc gcccgaggat aatcctttca tggcgggggc tttccacggg gtgggtgaac
     3961 ctgatttctc attgaacatc gggataagcg ggcccggggt cgtcaggagc gttgtggaaa
     4021 agaacaggga ctgcgacctt acggagcttt ccggggttat aaagcgcacc acatttaaaa
     4081 taacaagagc aggagagctt ataggaagag agctcgccca caatctttca gtacctttcg
     4141 ggattgtgga tatctcgctt gcatcaacca caaaggtggg tgattcagtg gctggaatcg
     4201 tggaaatgat gggtgttgag aaaatggggg cgccgggaag cactgccgcg ctggccctac
     4261 ttgtggatgc agtgaaaaaa gggggagcta tggcatcggg gaacattggg ggcttaagcg
     4321 gcactttcat tccggtgagt gaggattcct gcatgaatga agcggtccgg gttggcgcgc
     4381 tttccataga aaaacttgag gcgttcactg ctgtttgttc tgtagggctt gatatgatcg
     4441 cgattccggg ggatacaaag gcacagacca tttcagcaat tattgcagat gagcttgcca
     4501 taggggttgt taataataag accactggag taaggctcat tcctgtacct ggaaagaagg
     4561 caggggatta tgtgaatttc ggggggcttc ttggtgagac ttatgtgatg aacataagcg
     4621 ctttcagttc cgatgatttt atattgcggg gtggaaggat gccaggtcct gttacgagca
     4681 tgagaaatta ggcgaaaaga cacatcagga ggtctaccct tgatcccatg ttaaaaaagg
     4741 aagatttgag aaggtttata taagagtcaa tcgaatcaat aattggtatt gttttctgaa
     4801 aaggggaacc aaaagatgat caaatatgaa tgaatacata atttataaat atctggaatt
     4861 tctgattttt gctatatatt ttataatgat tttcctgtct gttcaaatat ggttaatctg
     4921 gcaaaacatc gacaaaaatg aattaagatc cagggtattc ataactgatc cttttataag
     4981 aaataatttt attattgttt ttttagtgag tatttttttc tttatgcatg aattttttga
     5041 agagacagaa cttcgaaatt catatttggt ttttgaattc tccgaactca tgggttttat
     5101 tttgatctta tatattacat atatgtggta ttcgaccctt aagacatgtg ctattaaaca
     5161 acctataaat gaaatcctgc ttaatgcacg catgaatcat caaaagttag agaaaaatat
     5221 ctctttttgg acaaatgata ataaatcaaa attcatattg gttttcggat ctttcttcac
     5281 aacactgggc attgctcttt ttgtccccgt atcaacaata atttttgctt taattatagg
     5341 ggtcttattt gttccacctg caatagcgct gatatcaaca c
//
LOCUS       DATGBN010000311         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_567760, whole genome shotgun sequence.
ACCESSION   DATGBN010000311 DATGBN010000000
VERSION     DATGBN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567760"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..>1742
                     /locus_tag="VKL21_07650"
     CDS             386..>1742
                     /locus_tag="VKL21_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB45679.1"
                     /translation="MNGEKKDSEKVSDLRKRTEDALQGKNEDLKGLSTTDARSLVSEL
                     QVHQTELEMQNEELRKAKKELEDVRNSYSDLYDFAPVGYFTFDKNGLIYKMNLTSAAK
                     LGMERGFLIKKPFSNYISKSSKDVFYSHLHQVFNTGKRQTCEIKLQDKKGIEFDAQLE
                     TIPEQEIEGNINRCKTIVIDITERKRAEKAAEELGRYAESIVETVREPLLVMDANLNI
                     ISASRNFYKSFKTTPEDTIGKFIYDLGNHQWDIPALRTLLEDILPKETKFESFEVKHD
                     FQTLGNKTMLLNAREIRREDIGTKTILLAIEDITERKRMEEKLEKILADFGRSNVELE
                     QFAYVASHDLQEPLRMVTSFTQLLEKRYKNKLDRDADEFIGFIVDGALRMQSMINDLL
                     QYSRIGTRGKPFIVTDFESILNQALINLKVTIEENNAIITHDPLPTLMADPSQMTQVF
                     QN"
BASE COUNT          594 a          307 c          392 g          449 t
ORIGIN      
        1 ctgggtaaaa tgtggtttgt tcaaaggaag gttgtagtac caatccataa aaaaacgtgc
       61 ttatgagttt gaagcggaaa tcactcaaga cagaacatca atagggaaga tcgctaagct
      121 tagctgcaat tcattgcttg atgaatacaa actctataaa aatatggtga gataattatt
      181 taattattgt cttatggaca ctttacggac gaatcgtggt gataccctta ataggcataa
      241 accaatatat gatcatgtat cggctacaag agaatgatgc tcagcctgat ccatcaggtg
      301 gaggcaaaga gatgatcctt cttgccattg aagatatcac agagcgagca gggaaaggat
      361 aatctcaaaa tcgggggata gagatgtgaa tggagaaaaa aaggattccg aaaaagtatc
      421 tgacctaaga aaacggacag aggatgcatt gcaggggaaa aatgaagacc tgaaagggtt
      481 atctaccacg gatgcccgga gtctggtcag cgaactgcag gtgcatcaga cagaactgga
      541 gatgcaaaat gaagaacttc gtaaggcaaa aaaggaactt gaagatgtgc gcaacagtta
      601 ttctgacctg tatgattttg ctcctgtagg ttattttaca tttgataaaa atgggctgat
      661 ctataaaatg aatctcacca gcgctgccaa attgggaatg gaaaggggct ttttaattaa
      721 aaaaccattt tctaattata tttctaagag cagcaaagat gttttttatt ctcatctgca
      781 tcaggttttc aacactggaa aaagacaaac ctgcgagatc aaacttcagg ataaaaaagg
      841 aattgagttt gatgctcaac tggaaaccat tccggagcag gaaatcgagg gtaatatcaa
      901 ccggtgcaag acaatagtaa tcgatatcac agaacgcaag cgggctgaga aggctgcgga
      961 agaactaggc agatatgccg aaagtattgt agaaacagtg cgtgagccac tattagtgat
     1021 ggatgcaaac ttgaatataa tttcagccag cagaaacttc tataaatcct tcaaaacaac
     1081 ccctgaagat actattggga aattcatcta tgatctcgga aaccatcaat gggatatccc
     1141 tgctcttcga acgttattag aagatattct tccaaaagaa acaaagttcg agagctttga
     1201 agttaagcat gatttccaga cactcgggaa taaaacaatg ctgctcaatg cgcgcgagat
     1261 ccgccgggaa gacatcggta ctaagacgat tcttcttgcc attgaagata tcacagaacg
     1321 aaagcgtatg gaagaaaagt tggaaaaaat tttggctgat tttggccgat cgaatgttga
     1381 acttgagcag ttcgcttatg tggcttccca tgatctccag gaacctttgc gtatggtaac
     1441 aagctttacc cagcttctgg aaaaacgata taagaacaaa ttagacaggg atgctgacga
     1501 gttcattgga tttatcgtgg atggagcttt acggatgcag agtatgataa atgatctttt
     1561 gcagtattcg cggattggaa cccgcggcaa accatttatt gtaacggatt ttgagtctat
     1621 actgaatcag gcattgatta atcttaaagt aactatagaa gaaaataatg caattataac
     1681 tcacgatcct cttccgactt taatggcgga tccctcgcaa atgacccagg tttttcaaaa
     1741 ct
//
LOCUS       DATGBN010000312         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_179705, whole genome shotgun sequence.
ACCESSION   DATGBN010000312 DATGBN010000000
VERSION     DATGBN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179705"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VKL21_07655"
     CDS             <1..1619
                     /locus_tag="VKL21_07655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HMB45680.1"
                     /translation="ASDVLGIDEKVYDFYYIIDISSPIIGGPSAGGALTVATIAALNK
                     WPLKPDVVMTGMINPDGSIGPVGGIPFKLEAAAEKNATLFLIPEGQSNVTVINTSTRS
                     RGALIITGEKEETVDVVELGKKLNVTVKEVNTIQDVVLEFTGHQISKPAYTGTILTTQ
                     YMDLLKPLAQNLKSESGNMYREMESTIQENQFTQTAKDMLDRADKMYADNKYYAATSL
                     YFNSMFTMRFAQWKDGYDKASDKEQFLSELEKRVDKQINVSEDQLKNFTFYGVSDVEA
                     VGAAESRITTARMRLDEAKKQNDTQDKISSLAFANERARTAQWWLTLSTPDGKIVPEK
                     ILKDRAGWYLSQAQSINTYMITLISESGSHPDIIGGTSEDIAHAQNEMERGYYSGAIF
                     DSLQATIKSSTSIGLMGKTDPQKKIDESNEAAKVAINEARNLGIEPTLAVSAYEYGET
                     MTSPFDRISQYSYAKMIAQTSIVLKSHSVPSDQIPVKPVITPYISEPFKMPEINATSK
                     KTGTKIEIPGFEAIMAISILLLARRLKKLH"
     gene            1652..>2737
                     /gene="pyrG"
                     /locus_tag="VKL21_07660"
     CDS             1652..>2737
                     /gene="pyrG"
                     /locus_tag="VKL21_07660"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HMB45681.1"
                     /translation="MKYIIVTGGVMSGLGKGITAASIGRILKNKGFNVTAIKIDPYIN
                     IDAGTMSPYQHGEVFVLKDGGEVDLDLGNYERFLDIELTREHNITTGQVYQTVIEKER
                     RGDYLGKTVQIIPHVTNEIKERIRKVSKKSGAEICIIEVGGTVGDIESMPFLEAVRQM
                     HSEEKEEDIIFVHVTLVPLDTQGEQKTKPTQHSVKELRSLGLQPDAIVARCKEPVLFD
                     TKAKIALFCDVPARAVVSAHDSSDIYYVPALMEKEGLSDYLMQKLKITEKEISHDWDT
                     LMKKMASIDREIRIAVVGKYSHLGDSYISINEALKHAGIECGCRVKAEWLDSEEFERK
                     PSDIDMLLKYNGILVPGGFGVRGTEGKI"
BASE COUNT          891 a          577 c          640 g          629 t
ORIGIN      
        1 tggcttctga tgtcctgggg attgatgaaa aagtatatga tttttattat attatcgata
       61 taagttcacc tatcattgga ggaccctcgg caggaggagc cctgacagtt gccacgatcg
      121 ctgccttaaa taaatggccc ctgaaacccg atgtcgtaat gacaggaatg ataaatcctg
      181 acgggtcaat aggacccgtg ggcgggatac cattcaagct tgaggcggca gcagaaaaaa
      241 atgccacact tttcctgata cccgaaggcc agagtaatgt aacagtgata aatacctcca
      301 cccgttcaag gggcgccctt ataatcaccg gtgaaaagga ggaaacagtt gatgttgttg
      361 agcttggcaa aaaactgaac gtcacagtaa aagaagtaaa tacgatccag gatgttgtcc
      421 tggaatttac aggccaccag atctcaaaac ccgcatatac gggcactatt ctcaccaccc
      481 aatacatgga ccttctcaaa cctcttgccc agaacctgaa aagcgaatcg ggaaacatgt
      541 atcgcgaaat ggaatcaaca atacaggaaa accagttcac acagacggca aaagacatgc
      601 tggacagggc agataagatg tacgctgata ataaatatta tgcagcgact tccttatatt
      661 ttaattcaat gttcacgatg cgatttgcac agtggaagga cggatatgat aaagcttcag
      721 ataaagaaca atttctttcc gaactcgaaa aaagggtaga taaacagatc aatgtttccg
      781 aagatcagct taaaaacttt acattttacg gagtaagcga tgtggaagca gtaggggcag
      841 ccgaatcaag gatcaccact gcaagaatga gactggatga ggcaaaaaaa cagaatgata
      901 cccaggataa aatatcttca ctcgcctttg ccaatgagag ggcgcgcaca gcccaatggt
      961 ggctcacgct ttcaactcct gacgggaaga tcgtgcctga aaaaatattg aaagaccggg
     1021 caggctggta cctgagccag gcacaatcca tcaataccta catgataacg cttatttcag
     1081 aaagcggttc ccatccggat atcataggcg gtaccagcga ggatatagcc catgcccaga
     1141 atgaaatgga acgcggctac tattcaggag cgatattcga ttcactccag gccacgataa
     1201 aatccagcac ttcgatagga ttaatgggaa aaacagatcc gcaaaagaaa attgatgaga
     1261 gtaatgaagc tgcaaaagtg gcgataaatg aggcccgaaa ccttggaatt gaacctactc
     1321 ttgcagtcag cgcatatgag tacggagaaa ccatgacctc tccttttgac aggatatccc
     1381 aatattctta tgcaaaaatg atagcacaga catcaatagt gctcaagtcc cattctgtcc
     1441 cctcggatca aatacctgtt aaaccagtca ttactccata tatatcagaa ccgttcaaga
     1501 tgcctgaaat caatgccacc tcaaagaaaa cagggacaaa aattgaaatt ccgggttttg
     1561 aagctataat ggctatatcg atattattgc tggcaagaag gctgaaaaaa ctccattaat
     1621 tatatccatt atgctatcct aaaaagtggt catgaagtat atcatagtca ccggcggtgt
     1681 gatgagcggg cttggaaaag gaataacggc agcctcaata gggcgcattc tgaaaaataa
     1741 aggttttaat gtgacagcta ttaagataga cccttacatc aatatcgatg ccgggaccat
     1801 gagcccatac cagcatggcg aggtctttgt cctgaaggac ggcggggaag ttgaccttga
     1861 ccttggtaat tatgaacgtt ttcttgacat agaactgaca cgtgaacaca atatcaccac
     1921 aggccaggtg taccagacag tcattgagaa agaaagacgc ggcgattacc tcgggaaaac
     1981 agtacagatc attccccatg tcacaaatga gatcaaagag cggataagga aagtttcaaa
     2041 aaagagcggc gctgaaattt gtataattga agtgggcggt acggtgggcg acatcgagag
     2101 catgcctttt cttgaagctg tgaggcagat gcacagcgaa gaaaaggaag aggatattat
     2161 ttttgtccat gtgacccttg ttcctcttga tactcagggt gaacagaaaa caaaacccac
     2221 gcagcactct gtaaaggaac tccggagtct tggcctccag cctgatgcca tagtggcgcg
     2281 atgcaaagaa cctgtacttt tcgacacgaa agccaaaatc gccctgtttt gcgacgtacc
     2341 cgcaagagca gtggtaagcg cccatgattc cagcgatatc tattatgttc ccgcgctaat
     2401 ggaaaaggag ggtctgtctg attatttgat gcagaaactt aagatcacgg aaaaagaaat
     2461 ttcacatgac tgggatacat tgatgaaaaa gatggcttcc attgacaggg agatccgaat
     2521 agcagtggta gggaaatatt ctcatcttgg tgattcctat attagcatca atgaagcgtt
     2581 aaaacatgct ggaatagaat gcggctgccg tgtaaaggca gaatggcttg attctgagga
     2641 atttgagaga aaaccttctg atatcgacat gctcctgaaa tataacggca tccttgtccc
     2701 cggaggtttt ggcgtgcggg ggactgaagg caagata
//
LOCUS       DATGBN010000313         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_451784, whole genome shotgun sequence.
ACCESSION   DATGBN010000313 DATGBN010000000
VERSION     DATGBN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451784"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="VKL21_07665"
     CDS             complement(<1..1561)
                     /locus_tag="VKL21_07665"
                     /inference="COORDINATES: protein motif:HMM:NF006217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HMB45682.1"
                     /translation="MNGEEAFYKKPRVVLLLIVILASIVAMTVSYKDGEVKVGSNLKY
                     GLDLEGGTWLQLQLQGAIVQVNADEKKIIQTEFGKLLNDSSINIEESTLNSITFTTSK
                     QTTQKIIDNLGFGKSTISQAPDGRTRISLQTNRDSVIQKFLENNLNAEIVSIPGNILT
                     FEVRKTVTEDELNALLQPVDGKVNSFKVGVSANTRDETKNILETKLNSLGIKEIPVRT
                     VGDNYILIDLAGVDMTTAKDIAAKPGKFEIRIQVKDNETEHVLYGTDITPGLPSKEKG
                     DMWGVSFTLNDAGAAALRDAAIQYGAVTDPEAHNLIMSLDENVVFSAPLSPELARNIQ
                     SVPVKSMVATTGAGDEGQRKATELQIHLRAGALPVKVDVVGAGQVSAELGSKFKLQIL
                     IAGIIALILVAIVVSLRYKQPNIVIPMLSTSFSEVIIILGFTVLVGFQLDLPTIAGII
                     AVIGTGIDHLIIITDEVLAGGAMPPDKVYRSRLTKAFAIIFSAAATVLVAMSPLLIMG
                     FGALRGFAVITI"
     gene            complement(1558..2499)
                     /locus_tag="VKL21_07670"
     CDS             complement(1558..2499)
                     /locus_tag="VKL21_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HMB45683.1"
                     /translation="MNLINEKAIDEYVNKFNFKQMLIFPAILLAVSLLILTYTAITSP
                     THTPVELGMEFQGGTAITFDSSKTPDQLREEFAAYPVVQAREYGGGNRKLLQFGPMKE
                     SDLSDITNKINSEYSNIEIRQMGEVVSKALQGQTLQAILFSFLGMALVVFIIFRTFVP
                     SIAVVISAFADIVFAAAMMDVFGIVLSLGTVAALLMLIGYSVDTDILLTTRLLKRKGE
                     LGDKIKDAMKTGMTMTLTTLAALIALFLVSSGSYLVSSFTRIDIIRDISVVLIFGLIA
                     DIINTWMTNVGILKWYLERGGTTEKRIEKPKKRRQTA"
     gene            2602..>3167
                     /locus_tag="VKL21_07675"
     CDS             2602..>3167
                     /locus_tag="VKL21_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45684.1"
                     /translation="MYTSYIYDGNYPQILILKPFDSCPQICVYCQRNWEITDIKDAVF
                     SRETMRNAINWIKDNQNIKEVLITGGDPLTLDDISIDWLLSEISAIAHVDRIRIGTRI
                     IVTMPQRITERLIEIFEKYHELGTRELCIVTHFEHPTEFTPDSLEAIKKIKKLGINLY
                     NQQVFTYYNSKKYESCFLRTVLKKSGIDP"
BASE COUNT          924 a          688 c          617 g          938 t
ORIGIN      
        1 cgatggtaat tacggcaaat cccctgagag cgccaaatcc catgataagc agaggagaca
       61 tcgccacaag cactgtcgca gcggctgaga atataatggc aaatgccttg gtaagacgtg
      121 atctataaac tttatccgga ggcattgccc caccggcaag aacctcatca gtgatgatga
      181 tgaggtggtc aatgccggta ccgataactg caattattcc tgctattgtt ggcaggtcaa
      241 gctggaaccc tacaagaacg gtaaaaccca gtatgataat tacttcactg aaggatgttg
      301 aaagcatcgg gataacaatg tttggttgtt tgtacctcaa tgaaacaact atggcaacta
      361 atatcaatgc tattatcccg gcgatcaata tctgtagctt gaactttgaa ccgagttcag
      421 cggaaacctg ccctgcaccg acaacatcca ccttcacagg cagggcgcca gcccttaaat
      481 ggatctggag ctcagttgcc tttctctgtc cctcatcacc ggcgcctgta gtcgctacca
      541 tgcttttcac tggtacgctc tgtatgttcc tggccagttc aggtgagagc ggagcactaa
      601 ataccacatt ttcatccagg gacattatca aattatgggc ttcaggatca gtaacagccc
      661 catactgtat tgctgcatcc cgcagagctg ccgcgccagc atcattaagg gtaaaagata
      721 caccccacat gtctcctttc tcctttgaag gcaacccggg tgttatatcc gtgccataaa
      781 gcacatgttc tgtttcattg tccttcacct gtatgcgaat ttcgaatttt ccgggttttg
      841 ctgcgatatc cttggcagtt gtcatatcta cacctgcgag atcaataaga atataattgt
      901 cacctactgt cctgacagga atttccttga tcccgagact gttgagttta gtttcaagga
      961 tattttttgt ttcatccctt gtatttgctg aaacacctac tttgaatgaa ttgacttttc
     1021 catccacagg ctgtaacaaa gcattcaatt catcttcagt cactgttttt cttacttcga
     1081 atgtaagaat atttccggga atactgacaa tttctgcatt aagattattt tcaagaaatt
     1141 tctggattac tgaatccctg ttcgtttgca gggaaatcct tgtacggcca tcaggagcct
     1201 gggatattgt ggatttacca aaccctaagt tatctattat cttctgtgta gtctgttttg
     1261 atgtagtaaa tgtgatggaa tttaaagtgg actcctcgat attgatggaa gaatcattca
     1321 gcaattttcc aaattccgtc tggataattt ttttctcatc ggcgtttacc tggacaatag
     1381 ccccctgaag ctgtaattgc agccatgtgc ccccttccag gtcaaggcca tatttcaggt
     1441 tactgcccac cttcacttct ccatctttat aagagacagt cattgcgaca atcgaagcaa
     1501 ggatcactat caataataag acgacacgtg gttttttata aaaagcttct tcaccgttca
     1561 tgctgtctgc ctcctctttt taggtttctc aattcttttt tcagttgttc cccccctttc
     1621 cagataccat ttcaggatcc caacattggt catccaggtg tttattatgt ccgcgatcaa
     1681 accgaaaata aggaccacag aaatgtctct gattatatca atgcgcgtaa acgatgaaac
     1741 cagataggaa cccgatgaaa ccagaaataa tgcgataaga gctgctagag tggtcaatgt
     1801 catggtcatt ccagttttca tggcgtcttt tatcttgtca cccaattcac cttttcgctt
     1861 gagaaggcgc gttgtcaaga ggatatctgt atccacagaa taaccgataa gcatcaaaag
     1921 ggcggcaacc gtacccaggg ataatactat gccgaataca tccatcattg ccgctgcaaa
     1981 cacaatatca gcaaacgctg aaattaccac agctatggaa gggacaaaag tcctgaatat
     2041 tatgaataca acaagcgcca tcccaaggaa tgagaataaa attgcctgta gcgtctgtcc
     2101 ctgcaaagct ttgctgacta cttctcccat ttgccttatt tcgatattgg agtattctga
     2161 atttatttta tttgtaatat cactgagatc ggattccttc atagggccga attgtaataa
     2221 tttacggtta cctccaccat attctcttgc ctggacaaca ggatatgccg caaattcttc
     2281 tcttaactgg tcgggagttt ttgaactgtc aaaagttata gctgttccac cctggaattc
     2341 cattcccagt tcaaccggag tatgagttgg acttgtaata gcagtgtaag taaggatcaa
     2401 aagtgatact gcaagcagaa ttgctggaaa aataagcatt tgtttgaaat tgaacttgtt
     2461 gacatattcg tcaatcgctt tttcattaat tagattcatg ggatctcctc tcgacaagtt
     2521 aagacctgct taaattataa cacaacatat ttaccttttg ctgtatcatt tccaataata
     2581 ctaacgatga gtttttgtat attgtataca agttatattt atgatggaaa ttatccgcag
     2641 atactcattc taaagccttt tgattcatgc ccgcagatat gtgtgtactg ccagaggaac
     2701 tgggaaatca cagatataaa ggatgctgtt ttctcaagag aaacaatgag gaatgccata
     2761 aactggataa aggataatca aaacataaaa gaagtcctta tcacaggagg tgacccgcta
     2821 acactagatg acatttctat tgattggttg ttgtcggaaa tctcggctat agcccatgtc
     2881 gataggatca ggataggtac aaggattata gtgacaatgc cgcagaggat cacggaaaga
     2941 ctaattgaaa tatttgaaaa ataccatgaa ttggggacaa gggaattatg catcgtcaca
     3001 cattttgaac atcccaccga atttacccct gattccctgg aagctataaa gaaaataaag
     3061 aaactcggta ttaaccttta caatcagcag gttttcactt attataatag taaaaaatat
     3121 gaatcatgtt ttttaagaac agtattgaaa aagtcaggga tcgaccc
//
LOCUS       DATGBN010000314         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_296310, whole genome shotgun sequence.
ACCESSION   DATGBN010000314 DATGBN010000000
VERSION     DATGBN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296310"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VKL21_07680"
     CDS             complement(<1..976)
                     /locus_tag="VKL21_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMB45685.1"
                     /translation="MIQKNRILIIFFIMVIISGITTIVSSGATSDIYVNQTGWWRADG
                     AFNASATQIQAAIDAATAGETIYVYNGNYTENVNVTKQLTLRGIGMPVVNASDSGSAI
                     NLSADGITLVGFTATGGGIYPEAGIRVNSNSNNLSGNNASNNGDGITLISSSNNTLRG
                     NNANSNNQNGIWLDYLSNNNTITNNNASSNTLRGIYLNSASNNTIYNNYFNNTNNSQD
                     DGYNVWNVTKTAGTNIIGGSWLGGNYWSDYTEADMDSDGLGDTLTPYNIGITNGGDYH
                     PLMTVGTIAPPITIISPVNTTYNNASIVLNVTTNQTANVTYSINGAENQ"
     gene            complement(1316..2248)
                     /locus_tag="VKL21_07685"
     CDS             complement(1316..2248)
                     /locus_tag="VKL21_07685"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMB45686.1"
                     /translation="MKSLITGCAGFIGSHLAEKLLREGHEVIGIDCFTDYYPRKTKEN
                     NIKNALGNKKFSFIEEDLVNLREFPEVDYVFHQAAQAGVRSSWGKDFEIYTRNNILAT
                     QKLLEYYKDRNLRKFVYASSSSIYGNVNELPIREEAPKNPFSPYGVTKLAAENLCNLY
                     YMNYGTPTVSLRYFTVYGPRQRPDMAINKFVNAALSGGVIEEYGDAKQTRDFTYISDA
                     VQANLQAAQSDVIGESFNIGGGSRISVIELVRLIKDAVGKDIQIKHIEAQKGDVRDTY
                     ADTTKARKLIGYIPEVGIRDGIKKYVVSCQALNK"
     gene            complement(2250..3440)
                     /locus_tag="VKL21_07690"
     CDS             complement(2250..3440)
                     /locus_tag="VKL21_07690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB45687.1"
                     /translation="MKIAYVLETFPKLSESFILNEIVELLKNGHDIRIFSIYLPVEDI
                     KHEVINEYNILERTHYFSFNRIFKVNLIILLKYFLMGVVQDLCNLKISINIFALDLKL
                     AYFATIMHKNHMELIHAHFASMGNISRRLGKILGLPYTLTAHAFDIYMDPDEDKLRKV
                     MENAHSVVTISDYNKNHLQGKIGIVNRIEVIRCGIDIDRFSPRRKLEIDDGIKLLTVT
                     RLVEKKGLEYLIRAIPIVIKQMPGFDLTIVGSGPLNDHLHQLVHDLKLEGYIQFKGDL
                     SDFELMHYYENAEMFILPCIIAENGDRDGIPVAIMEAMAMELPVISTTVSGIPELVDD
                     GISGILVSQKDEKAIANAIIKLHQDKKLRLEMGENGRKIIENKYNIVSESEKLIKIFN
                     KMRC"
     gene            3585..>3714
                     /locus_tag="VKL21_07695"
     CDS             3585..>3714
                     /locus_tag="VKL21_07695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB45688.1"
                     /translation="MVSYINSEKPLVSVIIPTYNRANLIGRAIQSVLNQTYRNIEII"
BASE COUNT          994 a          782 c          691 g         1247 t
ORIGIN      
        1 tttgattttc ggctccattt attgaataag tgacattagc ggtctggttt gtagtaacat
       61 tcagaactat tgaagcattg ttgtaagttg tattaacagg tgaaatgatc gtaatggggg
      121 gagcgattgt gcccacagtc atcagcggat gatagtcgcc accgtttgta ataccaatgt
      181 tgtacggcgt cagggtatcg cccagaccat cgctgtccat atcggcttcc gtatagtctg
      241 accagtagtt cccaccaagc catgaaccgc ctataatgtt cgtgccagct gtttttgtga
      301 cgttccagac attatatcca tcgtcctgtg agttgtttgt gttgttgaag tagttgttgt
      361 agatcgtgtt gttgctggct gagtttagat agatgccgcg tagtgtgttc gagctggcat
      421 tgttgttcgt gattgtgttg ttgttgctta aatagtccag ccagatgccg ttctggttgt
      481 tcgagttcgc attgttgccg cgcagcgtgt tgttgctgga agatatcaga gtgatgccgt
      541 cgccgttgtt cgaagcattg ttaccgctga ggttgttgct atttgaattt accctgattc
      601 ctgcctctgg ataaatgcca cccccggtcg cagtaaatcc aacaagtgta atcccatccg
      661 ccgacagatt gatcgcgctt ccactatcgc tggcattcac cacaggcatc ccgatcccgc
      721 gcagggtcaa ttgcttggtc acgttcacgt tctcggtgta gttgccgtta tacacataga
      781 tcgtctcgcc ggcagtcgcg gcatcgattg ctgcctgtat ctgtgtggcg cttgcattga
      841 aagcgccgtc cgcgcgccac cagccggttt ggttgacata gatgtcagaa gttgcacccg
      901 atgagacaat ggttgtgatg ccagatatga tcaccataat aaaaaatatt atgagaatcc
      961 tatttttttg tatcatcttt ctgcaatgct ctctgattga tgagaaaacg agatttccat
     1021 ttacctgcgg tgacaggtaa agatatctat ttttccatgt ttataattta atacagtaat
     1081 ataataaata tcatcataat gatgtatata tttttcggtt tcagacaaac gaacaatttg
     1141 aatccaacac tgatcaaacg gatgctcacg gatacgattg attcgttttc atattcaatc
     1201 aaaaagaaga aaacaaaaat gcttcacagc gaattatcag atagaaggat ttgaatcaga
     1261 acccccaaat ccgttcgtat cagtcgaatc cgcgctccct tcgatttgat gaatactatt
     1321 tattcaatgc ctgacaagac actacatact ttttaatacc atctcttatc ccgacttccg
     1381 ggatatatcc aatcaatttc ctggctttgg tagtatcggc atatgtatcc ctcacatctc
     1441 ctttttgtgc ttcgatatgc ttgatctgta tatcttttcc cactgcatct ttaattagcc
     1501 tgaccagttc aatgactgat atcctgcttc ctccgccaat attaaagctc tcaccaataa
     1561 catcgctctg tgcagcctgc aggtttgcct gcaccgcgtc tgatatgtat gtgaagtccc
     1621 ttgtctgttt tgcgtcacca tattcctcaa ttacgcctcc gctcaaagct gcattgacaa
     1681 acttgttaat agccatatca ggcctctgtc tgggcccata gaccgtaaaa tatcgcagag
     1741 atactgtcgg tgtgccgtaa ttcatgtaat atagattaca caggttctct gctgcgagtt
     1801 tggtcacccc gtaaggtgaa aatggatttt ttggtgcttc ttcgcggata ggcagttcat
     1861 ttacattccc atatattgaa gaagacgatg catagacaaa ctttctcagg ttgcggtctt
     1921 tataatattc cagcaatttc tgggtcgcaa gtatgttatt tcgagtgtat atttcaaaat
     1981 ccttacccca cgaggatctc actcctgcct gtgcagcctg gtggaataca taatctactt
     2041 ccgggaattc ccgaaggttc accaggtctt cttctatgaa ggagaatttt ttattgccaa
     2101 gtgcattttt tatattgttt tctttggtct ttcttgggta ataatcagtg aaacaatcga
     2161 tacctataac ttcatgtccc tctcgcagca atttttctgc cagatgcgaa cctatgaatc
     2221 ctgcacaacc tgttattaat gatttcattc taacacctca ttttattaaa aattttgatc
     2281 aatttttctg attccgatac tatattatat ttattctcta ttatttttct tccgttttca
     2341 cccatttcaa ggcgcaattt tttatcttga tgtagtttta taatcgcatt tgcaatcgct
     2401 ttctcatctt tttgggatac caatatcccg gaaataccat catcaacaag ctcaggtatc
     2461 cctgataccg tggttgatat tactggcagt tccatggcca ttgcctccat gatggcaact
     2521 ggaataccgt cgcgatcacc attttctgct atgatgcatg gcaatataaa catttctgca
     2581 ttttcataat aatgcataag ttcgaaatct gataaatctc ccttaaattg tatgtatcct
     2641 tctaatttca ggtcatgtac cagttgatga agatgatcat tcaaaggtcc tgagcccaca
     2701 atggtcaaat caaaaccggg catctgtttt atcacgatcg ggatcgctct tatcaggtac
     2761 tccaatcctt ttttctcaac aagtcttgta acggttaaca attttattcc gtcatcgatc
     2821 tctaattttc ttcgtggcga aaacctgtca atatcaatgc cacaccggat cacctctatt
     2881 ctattcacta tgccaatttt accctgcaaa tggttcttgt tataatcaga tatcgtaaca
     2941 acggagtgag cgttttccat cactttgcgt aatttatctt catccgggtc catataaatg
     3001 tcaaatgcgt gtgctgtcaa tgtgtatggt aatcccagga tcttacctaa tcttcttgaa
     3061 atattgccca tggatgcaaa atgcgcatgt atcaattcca tatggttctt gtgcataatc
     3121 gtggcaaaat atgccagttt taaatccaac gcaaatatat taatagaaat ttttaaattg
     3181 cataaatcct ggacaacacc cataaggaaa tatttcaata aaataattaa atttactttg
     3241 aatatccggt tgaaactgaa ataatgagtt ctttctagta tattgtattc attgatcact
     3301 tcatgcttga tatcttctac gggcaaatat atcgaaaaaa tccggatatc atgcccattt
     3361 ttcaataatt caactatttc attgagtatg aatgattctg agagttttgg aaatgtttcg
     3421 agaacgtaag cgattttcat atatttaatt acctatcctt gtttaccaga atataatttc
     3481 ttccttatag gaatggcctg acattagcat gtagaaaacg agaataattt aatatagcag
     3541 gttgatggtt tagaaagaat acatgatctg ttcatttatt atttatggtg tcttatatta
     3601 atagtgaaaa accattggtt agtgtaatta ttcctaccta taatagggcc aatttaatag
     3661 gaagagctat ccagagtgtc ctcaatcaaa cttaccggaa cattgagata atta
//
LOCUS       DATGBN010000315         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_24552, whole genome shotgun sequence.
ACCESSION   DATGBN010000315 DATGBN010000000
VERSION     DATGBN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24552"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VKL21_07700"
     CDS             complement(<1..1529)
                     /locus_tag="VKL21_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45689.1"
                     /translation="MAFNRTTTVGGKKYRQLVESKWDPEKKQSRIHVIKHLGRVMEED
                     GKEKLIPSQLKFDSVDHAYPVGELALFWKVAEEFEVQKSIIKAIGRDDKDTSMAILIL
                     AINQLLGRKSLTKIGEWVSETPIPRWTKIDTNKLNKDYFLSALDNVSAQNENMKSSYS
                     YTIQYNITNSWKKIIGNEPERYFFYQDITRIRWNGDTSILAENGYGAQIGRPHIGFGL
                     VVSKDSYMPVMGYPVRGSSPDKTTVGETIDNLSRWKMKNITLVWDRGFVSKPNIDYAR
                     EKNFHVLSGGPHTSKEVDDWITKYTDSEIEKRENILEMSRGNGVYCIEDTGELFGRNC
                     KIVVMLDPQRRNNSRVERDLLIQELESETSGKKIAELKKDLSPIVNPAKGRRGYEIDQ
                     DAEEQARKLDGRSLFFSTDIKMPGKELIRTYFQKDHIEKAFRYLRGNACLAPIGYQLP
                     NRVEVYLSVVNFISYELIAAILWKIEKFNLGIGYESLMEKASKIFEVEFSSKDSKLYR
                     WT"
BASE COUNT          506 a          360 c          266 g          655 t
ORIGIN      
        1 gtccacctat acaatttcga atctttcgaa gaaaattcaa cttcaaatat ctttgaagct
       61 ttttccatca aactttcata acctattccg agattgaatt tctcgatctt ccataagata
      121 gcggcaatca attcatacga aatgaaatta acaacagaaa gatatacttc taccctattt
      181 ggaagttgat atcctatagg ggccaggcat gcattgcctc ttaaatatct gaatgctttt
      241 tcaatatgat ctttctggaa atatgtcctt atcagctctt ttcctggcat tttaatatcg
      301 gtggaaaaga ataatgatct tccatcaagt tttcttgcct gttcttctgc atcttgatca
      361 atttcataac ctcttcgacc ttttgcagga tttacaattg gtgacaagtc ttttttcaac
      421 tctgcaatct tctttcctga tgtttcagac tcaagttctt ggataagcaa gtctctttcg
      481 acacgtgaat tattgcgtct ttgtggatcc aacattacaa caattttaca attacgtcca
      541 aataattctc cagtatcttc gatacagtaa acaccatttc cacgggacat ttcaagaata
      601 ttttctcgtt tttcgatttc agaatctgta tatttcgtaa tccaatcatc gacctccttt
      661 gaagtatgag gtccaccgga aaggacatga aagttctttt ctcttgcata atcaatgtta
      721 ggtttactga caaaaccacg atcccataca agcgtaatgt ttttcatttt ccaacgggat
      781 aaattgtcta ttgtctcacc aacagttgtt ttatctgggc ttgatcctcg aacgggataa
      841 cccatcactg gcatataact atcttttgat acgactaatc caaatccaat atgcggtctg
      901 ccaatttgag caccgtatcc gttttcagct aaaatagatg tgtcaccatt ccatcgtatc
      961 ctggtaatat cctgatagaa aaagtacctc tctggctcgt tgcctattat tttcttccat
     1021 gaattagtaa tattatactg aattgtatat gaatatgaac ttttcatgtt ttcattctgt
     1081 gctgaaacat tatctaatgc tgataaaaaa taatctttgt ttaatttatt tgtatcgatt
     1141 tttgtccatc ttgggattgg agtttctgat acccattctc caatttttgt cagggatttt
     1201 cgacctaata actgatttat tgcaaggatt agaatcgcca ttgatgtgtc tttatcatct
     1261 ctgccaatag cctttattat acttttttgt acttcaaatt cttcagcaac tttccagaat
     1321 aaagctaatt caccaacggg atatgcatga tctactgaat cgaattttaa ttgggatgga
     1381 attaattttt cttttccatc ttcttccata actcgtccca gatgcttgat tacatgaatt
     1441 cttgattgct ttttttctgg gtcccatttt gattctacaa gctgccgata cttctttccc
     1501 ccaactgtcg tagttctatt gaatgccata gttactaatt agtaactata agtataaaaa
     1561 tatttcggtg cgtaaaaaca atagtggtag tctgatagaa tgcgtttttc aggaggtcaa
     1621 tttagtttca tagtgaccaa atgtcccgat gttcaggttt aacaaaggct tcagctaatg
     1681 caaacccgat gcctgtcgcg cctcctgtaa tcaaaatagt attgccatta attttcatca
     1741 ttaatcactt tgtatttatt atcatttaaa acaatatgtt actaacg
//
LOCUS       DATGBN010000316         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_24605, whole genome shotgun sequence.
ACCESSION   DATGBN010000316 DATGBN010000000
VERSION     DATGBN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24605"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1115
                     /gene="meaB"
                     /locus_tag="VKL21_07705"
     CDS             150..1115
                     /gene="meaB"
                     /locus_tag="VKL21_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMB45690.1"
                     /translation="MAKPDIAILTTQLLKGDRRAGAKLITIVENDPMDAKKVISRIYK
                     NTGKARIVGITGPPGSGKSTLVNQLAFFLRQKGRTVGIIAVDVSSPFSGGAFLGDRIR
                     MKDLTTDKGIFIRSMATRGCKGGIARATCDTIKILDALGYDIILVETVGAGEEDVDIM
                     NASHTSIVITVPGLGDDIQANKAGMMEIADIFVVNKADREGADMAACVLESMLRLTDK
                     HGWKPPIVKTIATGSVGINELSDRIDEHFNYLKDNDLIREWQRRRAEMEIVNIARDLI
                     TRDLEEIKKSEDYNKLIEEIIEKKTDPHSAVEEIMMMVKSRYTKK"
     gene            complement(1135..1572)
                     /locus_tag="VKL21_07710"
     CDS             complement(1135..1572)
                     /locus_tag="VKL21_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45691.1"
                     /translation="MKFKIFHPNNEIYYLVCPVNPSVYARFAKYMYKVYPKYNCIIPP
                     HIKNLMMQLADEYDLKKVDENNPYVRKVGWSTQATEEEKVFWQHCKNPHVRFYIDLNH
                     DFGKGNGVLTLMPLTFLNLLISFFSFFIYYTLKKRIRYNLHRF"
BASE COUNT          619 a          303 c          395 g          524 t
ORIGIN      
        1 ataaattttg ataatatgcc gttttattag gaaatgacat caattagacc tctaaaaata
       61 ttataatcaa ttaatattta aatcaataaa actttaaaat cgatagctat agacggtggg
      121 agtgaattta gaaactatgg agaaataaga tggccaaacc agatattgca atccttacaa
      181 ctcaactttt aaaaggggat aggcgggcgg gtgcgaaatt gataaccata gttgagaacg
      241 atccgatgga tgcaaaaaaa gtaataagca ggatatacaa gaacaccggg aaggcccgga
      301 tcgtgggaat aacaggcccc ccaggatctg gtaagagtac tctcgtaaac cagcttgctt
      361 tttttctgag acagaaggga agaactgttg ggattatagc agtggatgtt tcatctcctt
      421 tcagcggtgg cgctttcctc ggcgacagga taaggatgaa ggatcttaca actgacaagg
      481 ggattttcat aaggagcatg gcaaccaggg gatgcaaggg cggaattgcc agggcaacgt
      541 gtgatacgat aaaaattctt gacgctctcg gatatgatat aatactcgtt gagactgttg
      601 gtgcaggcga ggaagatgtg gatataatga atgcctctca tacgtcaata gttattacag
      661 ttcccggtct cggggatgat attcaggcta acaaggctgg aatgatggag atcgctgata
      721 tttttgtggt caacaaggca gatagagaag gtgcagacat ggcagcctgt gtgcttgagt
      781 caatgcttcg tttaactgat aaacatggat ggaaaccgcc tattgtgaag acaatagcca
      841 caggatctgt tggaataaat gaactttccg acaggataga tgagcacttc aattatctga
      901 aagataacga cctcattaga gaatggcaga ggagaagagc ggagatggaa atagtgaaca
      961 tagcaaggga cctcataaca cgagatctgg aggaaattaa gaagagcgag gattataata
     1021 aactcatcga ggagatcatt gagaaaaaaa cagacccgca ttctgcagta gaagaaatta
     1081 tgatgatggt gaaatccaga tataccaaga aataatgata tttccgtgat gatattaaaa
     1141 acgatggagg ttgtagcgga ttcttttttt aagggtataa taaataaaaa aactaaagaa
     1201 agatattaat aaatttaaga acgttaaagg cataagagta agaacgccgt tgcctttacc
     1261 gaaatcatgg tttagatcaa tataaaaccg aacgtgtgga ttcttacaat gttgccagaa
     1321 tactttttcc tcctcagtag cctgcgtact ccaacctact tttctgacgt atggattgtt
     1381 ttcgtcaacc tttttcaaat cgtattcatc agccaattgc atcatcaagt ttttgatgtg
     1441 tggaggaata atgcaattat atttcggata tactttatac atatactttg caaacctggc
     1501 atacacgctt ggattaacag gacaaaccaa ataatatatc tcattattgg gatgaaatat
     1561 tttgaatttc attgcctcct taaaccagaa ataaatgtcg gcatttttat gtcggtattc
     1621 aggtaccagg cctgcctccg ctctgaatat gactaatggt ttatcattta tacgtttttc
     1681 aaaacgatga acagcaaaat atcctactat ttcttcttta tttttatata taaatatttt
     1741 tgtaacttta gccggggaat taactacgta atgatcaaat tcttttgcat taaggccttc
     1801 aaaaatacgt ttatgtactt tgtacgtaac tattcagcct a
//
LOCUS       DATGBN010000317         5354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_529954, whole genome shotgun sequence.
ACCESSION   DATGBN010000317 DATGBN010000000
VERSION     DATGBN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529954"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1779
                     /locus_tag="VKL21_07715"
     CDS             238..1779
                     /locus_tag="VKL21_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45692.1"
                     /translation="MMSDEDLEGDFKTSFKIISNTQDIPFSYERSKDGLLTTTASDIK
                     LDRAVDAELIEIIERNIPYKQIIYRGGPEAIRDGELKILQPSKDGAGLIDVRIGAFKC
                     QRCGDETYIPQTNGKLIEPFECQNEDCGRKGPFKPLFPTELNKPVWKLPFAALKCSAV
                     GVYEDIYNYIREYLVLKPEEYHLMTLWIMASYLVDNFETVPYLLFIAPKESGKSQAMR
                     ILSQLAYRAFLAASVTPSALFRAIELWKVTLLIDEAEYQIKTDTESGQALYETLNMGY
                     KRDSFALRVEGENRIPARFDVFGFKAIASTKIFLPTLESRSIIINMSQGKPKEIIIDA
                     LRADIIRSELLYFKFFNLDKLPITQPDSNSGRIIEIMTPLFTVAQIFKGMNGAKTAIT
                     FDEITTLLCDKVKEMETSRADEEHESNEAMIIEKIKVRRSEKNISLEQISRDLGWYNE
                     TDSANNKQASARIGRTLKAMGIKTHRTGKGVLVKMYEDGNDERIDAMIKRYLDKSEQE
                     KGKEA"
     gene            2678..2845
                     /locus_tag="VKL21_07720"
     CDS             2678..2845
                     /locus_tag="VKL21_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45693.1"
                     /translation="MSSPAQLTKRVEDLEVTEKPQEPSVIVCEPDEVDTLKAKYPNII
                     LIVDDIPRSES"
     gene            2842..2994
                     /locus_tag="VKL21_07725"
     CDS             2842..2994
                     /locus_tag="VKL21_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45694.1"
                     /translation="MKQLEKRIEDLEQGKPGGLRDLVVSWENEPPFVIFHMQGARYIG
                     QTNHED"
     gene            2995..3177
                     /locus_tag="VKL21_07730"
     CDS             2995..3177
                     /locus_tag="VKL21_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45695.1"
                     /translation="MTIGTLTKRLDTLEARQPVKTHHVLLSEKIALYEKYFSGEIPIP
                     EEFKEQFDRFKKQLLQ"
     gene            3344..3652
                     /locus_tag="VKL21_07735"
     CDS             3344..3652
                     /locus_tag="VKL21_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45696.1"
                     /translation="MNTEPTPEQIARTAMAKVAMVTDPNLSEQQKALLYEIHMPEMPG
                     RQQHNGGKRHILPGGQVLDIVEDGRNQKQTYESSRSNNGITATATKRETINGFLVDFV
                     "
     gene            4135..4269
                     /locus_tag="VKL21_07740"
     CDS             4135..4269
                     /locus_tag="VKL21_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45697.1"
                     /translation="MAKNEDFHPQLYNIWNFARNNNETKHFGNVPTEITENLLYAVFA
                     "
     gene            4318..4626
                     /locus_tag="VKL21_07745"
     CDS             4318..4626
                     /locus_tag="VKL21_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45698.1"
                     /translation="MVEKRQLNIRLPVDLYDKMSNSGESMTSIIEQALREHYDTSNVS
                     DSSKDVSHLLTEVEYLRSENVKLLELLSREQIKSMDMQKKLMPAQEEIIKKNWWQFWR
                     "
     gene            complement(4661..4957)
                     /locus_tag="VKL21_07750"
     CDS             complement(4661..4957)
                     /locus_tag="VKL21_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45699.1"
                     /translation="MLLLYFPGGIVKDSQTGRKSIYKLYNENGTFAEFSKPQEPLATL
                     PNPLIPKIEPINTRQEVAKIAKVSKAQSITVDRCAPLYVEFKIRMWTRVIMNSV"
BASE COUNT         1877 a          985 c         1085 g         1407 t
ORIGIN      
        1 ttacttcagt aataaaggga tgcaggctgg gagaacatca ggacgtcttt cggtagttta
       61 tccacatatg cgatcatcca agacgataac caatgtcagt cgatttcatc gaaaaactca
      121 aaactattcg gagatactgt gtagtggccg aggcctaaca tgtaactgca atttagagga
      181 ctgctctcat gcacctcatt gtgcggtttg gttaaaatcg gatcatagtt tggtgcgatg
      241 atgagcgacg aggatcttga aggagatttc aaaacctctt ttaagatcat ttcaaacact
      301 caggatatac ccttttctta tgaacgttca aaggatggac tattaaccac aactgcaagc
      361 gatattaaat tagatcgtgc ggttgatgct gaattaatcg aaattataga acggaatatc
      421 ccgtataaac agatcattta taggggcggc ccggaggcta tccgggacgg cgaattaaag
      481 atcttgcagc caagtaagga tggcgcgggg ctaatagatg ttaggatcgg ggcgtttaag
      541 tgccagaggt gcggagatga gacatatata ccacaaacca acggcaaact tattgagcca
      601 tttgagtgcc agaatgaaga ctgcggacgt aagggaccat ttaagccgtt atttccgaca
      661 gaattaaata agccagtatg gaaattaccc tttgcagccc ttaaatgttc cgcagttggt
      721 gtgtatgagg acatctataa ttatatccga gagtatcttg ttttgaagcc agaagaatat
      781 catttaatga cattgtggat aatggcttct tacctggtag ataattttga gacagtcccg
      841 tatctgttat tcatcgcacc aaaggagagc ggaaaatcac aggcaatgag gatattaagt
      901 caattggcat atagagcttt cctggctgca agtgtaacac catccgcgtt atttagggct
      961 attgaattgt ggaaggttac gctattgatt gatgaagcgg agtatcaaat taagactgac
     1021 actgaaagcg gccaggcatt atacgaaacg ctgaatatgg gatataaacg ggattcattc
     1081 gcgcttagag ttgaaggcga aaatagaatc cctgctagat ttgacgtatt cggattcaag
     1141 gcgatagcat cgacaaagat ctttcttccc actcttgaaa gcagatctat tataatcaac
     1201 atgtcccagg gaaagcccaa agaaataatt atcgatgcac tgagagccga cataatcagg
     1261 tctgagttat tatatttcaa gttttttaat ttagataaat tacccataac tcagccagat
     1321 tcaaacagcg gacgaattat tgaaataatg acacctctct ttaccgtcgc tcagattttt
     1381 aaggggatga atggggcaaa aaccgcaatc acttttgatg aaattactac actcctttgt
     1441 gataaagtga aagagatgga aacttcacga gctgacgaag agcacgaaag taatgaggct
     1501 atgataatag agaaaattaa agttcgtcgt tcagaaaaga acatttcact tgaacaaata
     1561 agtcgagatc tcggttggta taatgaaact gactcggcga ataacaaaca agcatcggcc
     1621 agaatcgggc gaactctgaa agctatgggt atcaaaacac atagaaccgg aaaaggagtt
     1681 ctcgtgaaaa tgtatgagga cgggaacgat gaaagaatag atgctatgat taaaagatat
     1741 ctggataaaa gcgaacaaga aaaaggaaag gaggcttaaa atggtttatt tttcaagaat
     1801 aaacgataat aaaacgataa tgtatgaaaa tgaatcaaaa tgtatgttat attcaatcat
     1861 tcatgcgtta cacataacga aaatgtatga atgtatgatt tttctatata tgttatatct
     1921 aaaaaaaaat aaacatcatt catttaagcg aaattttaca tatatgggtc ttacccccct
     1981 aaatcattca tctcattcat tttcaatagg cgtgaggcat gaatgatcta tttaatcatt
     2041 cattttccat acatggacca tacatgcttc aaggtgagct catgaaatct gatgttatta
     2101 ctattcgcgt aactagattt aagcagggac ctggctggaa attccataaa gatattgaaa
     2161 gacaaggaag gaaaactcta ctcagggcat taggtgaaat atgaaccaag aacaaccaaa
     2221 aaaagacgat aaaaatacga acgaaaaaga tcagagacaa acccgataca gtgtaaaaaa
     2281 gattagatat ttaaggacaa aacatgtata aaacgccctt ttccctcctg caaagttttg
     2341 aaacttcgca caattcggca aatttccaac ctttctttat acgctcatca aacaaaacta
     2401 atgaatagag gatttgaaaa aacatgaaaa agatactaca agaacgtaaa tatcgcatca
     2461 atttagccga ttttacgata agaaaaacat atctgagtca aatgatcaca gaacttaata
     2521 atcaaagcct gagcgcagat ttaacggcga gcgaacgaaa taaaatatac aattcaatca
     2581 ctcaggcaac aaaagtccta aatgaaatct gcaaagattc ttcaattgaa gaattggaaa
     2641 agcggatctc acttttggaa gaatcgagga agaaaccatg agctcacctg cacaactgac
     2701 caagagagta gaagatcttg aggttacaga aaagccgcag gagcctagcg tgattgtgtg
     2761 cgaacctgac gaagtggaca cgctcaaagc aaagtatcct aatataattt taatagtcga
     2821 cgatatcccg cggagtgaat catgaaacaa cttgaaaaaa gaatcgagga tcttgagcaa
     2881 ggcaaacccg gagggcttcg ggaccttgtt gtatcctggg agaatgaacc gcccttcgta
     2941 atctttcaca tgcaaggcgc gcgctacatc ggccagacca atcatgagga ttaaatgaca
     3001 atagggacac tcacaaagcg gcttgatacc ctggaagccc ggcaacccgt caaaacgcat
     3061 cacgtgctct tatccgaaaa gatcgcactc tatgaaaaat atttcagcgg agaaataccg
     3121 atcccggagg aattcaaaga acaatttgac cgctttaaaa aacaattact acaatgaaaa
     3181 cggcaacagt agaaaggttt aaataatatg acagcattaa tagatataaa aggtgagaaa
     3241 attaatcgaa tagaaactct gatacaaaaa gagaaggcaa aggcagaaaa gcaattaaaa
     3301 gattctcaag aacgcaacag tcaaaaaaat aagaggtact aaaatgaata cagaaccaac
     3361 acctgaacag atcgcacgaa ccgcaatggc gaaagtagca atggttacag acccaaatct
     3421 ttcagagcag cagaaagccc ttttgtatga aattcatatg cctgaaatgc caggtcgtca
     3481 acagcataac ggaggcaagc ggcatatctt accaggcggc caagttcttg atattgtaga
     3541 ggatggcagg aatcaaaaac agacctacga gagcagcagg tcgaataatg gcataacagc
     3601 aacagcaaca aagcgagaga ctattaatgg ctttttagtc gattttgtgt gaattaaagg
     3661 ccgatgaact accaaagatc tgtccttcca tacacagcaa tctgttcctt tggcggcgag
     3721 gctcacattc tttttagcct aaacctatct taccttttta cacagtttaa aacgattatt
     3781 cactatgcaa agcaactgtc aaaaaagcac tattttttaa agaatctgcc gcgataggca
     3841 gcggtgcgat ggcagtgcat ataatacatc ttatacgcag ttttgagtat attggtttcc
     3901 gttaattaca ctttacgatt ttcctgtttt ctttgcagta tcttctacga acgtcggggt
     3961 aattgttacc gtcacttgct tctgcaacag caattgacgt ccttcgtttt aaggatagag
     4021 acagcaaggt cattgatttg tggctacaat gctacacgca ggactttata gctaaacact
     4081 taactttcag aaggaacggt taataatatc attgaaaaac tcaaaaccgg ccaaatggcc
     4141 aaaaatgagg atttccatcc tcaactttac aatatctgga acttcgccag aaacaataat
     4201 gaaacaaaac attttggtaa tgttccaaca gaaataacag agaatcttct ctatgcagtt
     4261 tttgcctaag cacatatata gcaccttgat acatttctga tacgttggtg atacggtatg
     4321 gtagaaaaaa ggcagctaaa tattagactt cctgtcgatc tctatgataa aatgagtaac
     4381 agtggagaga gcatgacaag tatcattgag caagcactta gagagcatta tgatacatca
     4441 aatgtatcag acagtagcaa ggacgtatca cacctgctta cagaagtgga atatttacga
     4501 agtgagaatg taaagttgct tgaactttta agtcgtgaac aaatcaaatc tatggatatg
     4561 caaaaaaagt taatgccagc ccaagaagaa attatcaaaa agaactggtg gcagttctgg
     4621 cggtaagaaa aaaccactca atcgtaatct ttatacatgg tcatacacta ttcattatta
     4681 cgcgcgtcca cattcttatt ttgaattcca cataaagggg cgcgcaccta tctacagtta
     4741 tcgactgtgc cttcgatact tttgctatct tggcaacttc ttgacgggta ttgattggtt
     4801 ctatttttgg aataagaggg tttggcaatg ttgccaaagg ctcttgtggt ttgctgaatt
     4861 cggcaaaggt tccattttcg ttgtagagtt tgtatattga tttcctgcca gtttggctat
     4921 ctttgactat ccccccggga aagtaaagca agagcaaatt tgtccgctta tcggagttgt
     4981 accttcatta gtgccacata tgcaactttg taaatttctg gtttttatta caggtggctg
     5041 cgacgcatct ccgaaaattt caggatcttc agattcttcc aaatctaaga atataatcac
     5101 gctctgaatt tatgattcaa aattcaaagt aacacaaatg aaaaaaagca caatgcatag
     5161 gtacatataa attaaaaaga ttcaaaagtt gcatatgtgg cactaatgtc ggtacaactc
     5221 ctaaaggagt tataacgata atagacacac gtgaagagat tgctaaaatt gctcaagtat
     5281 ttcatggtac tgtagctaaa gttaaattca ttgaaaaaaa cgctatgctg caacatgtag
     5341 atttttgcag acaa
//
LOCUS       DATGBN010000318         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_452536, whole genome shotgun sequence.
ACCESSION   DATGBN010000318 DATGBN010000000
VERSION     DATGBN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452536"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="infB"
                     /locus_tag="VKL21_07755"
     CDS             <1..1028
                     /gene="infB"
                     /locus_tag="VKL21_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMB45700.1"
                     /translation="LLEAEMLELKANPDKPALGTVIESSLDKGRGFTATVLVKAGTLR
                     IGDVMLAGSCFGHIKAMYNERGNKVDVVGPATPTLVLGLNGAPQAGDKFNVMESDREA
                     KDIATKRAQLQREQGLRTQKHITLDEIGRRIAIGNFQELNIIVKGDVDGSVEALSDSL
                     IKLSTPEIQLNIIHKAVGQISESDVLLAAASNAIIVGFQVRPSVSARKLAEKEEIDVR
                     LYSIIYNAIEEIRSAMEGMLMPEVREEIVATLEIREIFKVTKVGTVAGCYVKEGKITR
                     NTRIRIIRDGIVIYTGELGSLKRFKDDVKEVVGGYECGLNIHNFNDIEVGDIVEGYQE
                     FEVKKTL"
     gene            complement(1023..1397)
                     /gene="gcvH"
                     /locus_tag="VKL21_07760"
     CDS             complement(1023..1397)
                     /gene="gcvH"
                     /locus_tag="VKL21_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMB45701.1"
                     /translation="MNVPVNLLYTKDHEWLRVEGNLGYVGVTDFAQGELGDVVFIEIE
                     TVGETLMKEEVFGTIEAVKTVSDMFMPVGGEILEVNPLLAESPQVVNKDPYGKGWMVK
                     IKISNLSEVNDLLSEGKYKALL"
BASE COUNT          523 a          336 c          363 g          480 t
ORIGIN      
        1 tacttcttga agcagagatg cttgaactaa aagctaatcc cgacaaaccg gctctcggaa
       61 cagttattga atcttctctc gacaagggaa ggggatttac tgcgactgtt ctggttaaag
      121 ccggaactct ccgaatcgga gatgttatgc tggcaggcag ctgctttgga catatcaaag
      181 ctatgtacaa cgaaagagga aataaggttg acgtagtcgg gccggccact cctacacttg
      241 ttctgggatt gaatggcgca ccccaggcgg gcgataagtt taacgtgatg gagagcgatc
      301 gtgaggcgaa agatattgca acaaagaggg ctcaactcca gagagaacag ggtctccgta
      361 ctcagaaaca tattacactc gatgaaatag gccggagaat tgccataggt aatttccagg
      421 aacttaacat aatagtgaaa ggtgatgtcg acggttcagt agaagctttg agcgactcac
      481 ttattaaact ctcaacacct gagatccagc ttaatattat tcataaggca gtcgggcaga
      541 tttcagaatc tgacgttctt cttgctgcag cttcgaacgc cattatagtc gggttccagg
      601 taagaccatc tgtgagcgcc agaaaacttg ctgaaaaaga ggagatcgat gtgcgtctct
      661 attcaataat ctataatgct attgaagaga tacgatccgc tatggaaggt atgctcatgc
      721 ctgaagtgag agaagagatt gtagcaactc ttgaaatacg tgagatcttc aaggtcacga
      781 aagttggaac tgttgcaggt tgctatgtta aagagggcaa aataacaaga aataccagga
      841 taagaattat tcgcgatggt attgtaattt ataccggaga actgggatca ctcaaacgat
      901 tcaaggacga tgtgaaagaa gtcgttggtg gttatgaatg cggactcaat atccataatt
      961 tcaacgacat agaagtaggc gatatagttg aaggctacca ggaattcgag gttaaaaaga
     1021 ctctataaca gtgctttata cttaccttcc gaaagaagat cattgacctc tgaaagatta
     1081 ctgatcttta ttttaaccat ccagcctttg ccataaggat ctttgtttac aacctggggt
     1141 gattccgcaa gtaatggatt cacttccagt atctcaccac ctacaggcat aaacatgtca
     1201 gagactgttt ttacagcttc gatagtgccg aacacctctt ccttcataag tgtttcgccg
     1261 actgtttcga tctcaatgaa aaccacatca cctaactcac cctgagcaaa atctgttact
     1321 ccgacataac caagattacc ttctacccta agccactcat ggtcttttgt gtacaataaa
     1381 tttacaggaa cgttcatatt tgatatggtt ttagataatt atttgaattc atttctgcct
     1441 tcagtcagtc cagccaaaaa taaggctttt tttgtttaaa aagattgaca aatgacattt
     1501 tttgaattat taaaaaggat attaaaaata gatatttatg tcatgttaac tttctttttt
     1561 aagacaattc ccgagagcgg gttaagtctg attccattat tgcgatatct ccctttggat
     1621 caggcccaaa attccggtca atatgagtat agttccttgc atcatattgc ttcttttgcc
     1681 caagcaggaa cagttgcaag ag
//
LOCUS       DATGBN010000319         9935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_180710, whole genome shotgun sequence.
ACCESSION   DATGBN010000319 DATGBN010000000
VERSION     DATGBN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180710"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VKL21_07765"
     CDS             <1..348
                     /locus_tag="VKL21_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HMB45702.1"
                     /translation="ELGAFALKGNAHHVKMNSYCIVFGIQSLVNSLAGGSTPEDVAAA
                     ACHSVAEQLFEQQLQEVDVKEPVIMVGGTSLIQGLPKAVEELLKVKVIVPDNAQYIGA
                     VGAALLASGFISK"
     gene            355..960
                     /locus_tag="VKL21_07770"
     CDS             355..960
                     /locus_tag="VKL21_07770"
                     /inference="COORDINATES: protein motif:HMM:NF021412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HMB45703.1"
                     /translation="MMEQPETFIVESPDATGARTTRSLILDTVSDLSLSRVIVRLKVF
                     VDPVEPVFILAALLRLGSPSIKLKDFAKVDMGALGKDEVKIELDKELFAVKLLNKLWA
                     KYGKNNIEQPDRKIIIVKTDPIKDLDMLRELVIEEPQQEVLDRLIDAIALRIIPEGFR
                     VRKHELSNTHVLFVASEDTLKPEWLAKGKEIVDSLRREENA"
     gene            953..1879
                     /locus_tag="VKL21_07775"
     CDS             953..1879
                     /locus_tag="VKL21_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HMB45704.1"
                     /translation="MLEPMMYIGGAYKHSQLIELVEDLGGYILQKNIMQSEVVLLMLV
                     PINDLAIVETKSKELLGKLTRAPLTGSEIATVAPTLAYHHLPHLDCDVAEFLRKQGAK
                     TNMVGLARGVGKRICQLTAFERDLINEHDVAVFILGNYEHCIIEKSKKLYEGIEIPIV
                     VTGAPELRTEDVPNATAYVGSFGRILHHMRHAEELRTLDKLSAVIGKVLDDVRIEIAK
                     DPPSVAPPRIKKEIEEQIPEIVDNLHPLPITLQMGGVRVKLPYEEYHEQIENIKFVEG
                     VSMKEVARVLPSKMRNFILIKAIPKSETGFMI"
     gene            complement(1960..2457)
                     /locus_tag="VKL21_07780"
     CDS             complement(1960..2457)
                     /locus_tag="VKL21_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate pyruvate-lyase family protein"
                     /protein_id="HMB45705.1"
                     /translation="MQDVLKRPDIPACLRICAGTDGSVTQLLEVLTGKSVIVETISQN
                     VVKASPQIAGLLDIEIGDDVNDRLVTLKVDDLVYVLAKSLAPINRIPKGIRDDLMRAD
                     IPIGRILREHKLETRRDILNIRIVHRDFFGELPVLSREYKIIYENKVLMWINECFPVD
                     GRWRI"
     gene            complement(2460..2774)
                     /locus_tag="VKL21_07785"
     CDS             complement(2460..2774)
                     /locus_tag="VKL21_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HMB45706.1"
                     /translation="MIKEYSFKRGFSQDIGRTRSVLEKNFPAKVKEENGKLTITYGAF
                     SRLTVSIENKKLSVDSESNKEIKDNEVLDTNKRFRIFLEETTGYTAKERLKQAKKAVQ
                     GD"
     gene            complement(2852..3475)
                     /locus_tag="VKL21_07790"
     CDS             complement(2852..3475)
                     /locus_tag="VKL21_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45707.1"
                     /translation="MLQGNTDKNKVKGFDFTGNMALLVDDIIKTHPFFKHIRIENILV
                     AISPSNGNMNGVVAKLRPMLFEGGSKTKVVRNIEYAAPEIIIDGINILYIVYFHLPRF
                     LNHGNQKTKLSTVIHELHHVSPLFNGDIRRYLGKNYAHGNSRKDFDDLISIYTDEYIS
                     YTIHPELSIFLKYKYNELKRKYGAVYGDMIRIPRLKKIYPSWQTLTL"
     gene            complement(3447..4670)
                     /gene="pgk"
                     /locus_tag="VKL21_07795"
     CDS             complement(3447..4670)
                     /gene="pgk"
                     /locus_tag="VKL21_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMB45708.1"
                     /translation="MTRDYLTIDDIDIKSKTVLCRLDLNSPMDPNGIILDDSRFRSHI
                     ITLRELEDSKVVILSHQSRPGKSDFTTMEPHARQLSKLMKRNINHIDDIFGSHAIEAI
                     KNMNKGDIILLENTRFYSEESLERAPKDHKKTHMVRRLASVCDIFLNDAFSVSHRSHL
                     SVTGFTETLTSIAGRIMEKEIDSLNRGMSCSERPCVYVLGGTKVDDSIKVTNNVLERG
                     CADRVLVTGVVANVFLAASGLNIGESNMSFIDKQGYLPQIEIAKKLLKRFPDNIGLPV
                     DVALNKNDERIEEKISNLRPELPIHDIGIETMVKFSREISSAKTVVMNGPAGVFENEP
                     FALGTYELIKAGAKSGFSVIGGGHIAAAAEQMGISNKFSHVSTGGGACIDFLAGEKLP
                     GIEALKDAARKYRQK"
     gene            4821..5906
                     /locus_tag="VKL21_07800"
     CDS             4821..5906
                     /locus_tag="VKL21_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45709.1"
                     /translation="MRNLNEPITKKRKNMIKISLRYKVALELYASLSLFAAIIIILYY
                     SLFDMIGENLYVLSMGIIGFFIIAFIFFLRTLITDVNSVKFENDNSKDDPNIVEIRII
                     NKVALELISSISNIIILAAIFVFWITKPGGTSSEVILMLFIIILLIPMLILLKSLIFD
                     FNIIFMDKKHRDNFLHQLINRKNLVFFVIAIIIIFSIFASNKLTTSVGNLYQKSMSTG
                     EETITDISSNLYITSIAGERKNVSKGPIVGLTVYIEAMDLDFNFNSITVKFIDKTTTS
                     TLEYGIEADELHFYYEGKKTGKGKTVLKKGDMGIIQINLSSTKQELYSLDRGKMQLVL
                     GGGKTISRDFRVPEFKGESRILLYSTD"
     gene            complement(5896..6744)
                     /locus_tag="VKL21_07805"
     CDS             complement(5896..6744)
                     /locus_tag="VKL21_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMB45710.1"
                     /translation="MIPPTLPILDNHMHLNPSGRCLDAVREFARAGGTHIVLVSLPPW
                     SLGIEINSPEDYRKVFDRILKIARRAEEAEKVKVFVVLGVHPAELTKYYGRLGISRTV
                     EIMRGGLEIASEYVVEGKAIGLKSGRPHYEVEPKIWDHSNDIMRYSFELARDSGCAVQ
                     LHTESATEEGLAQIVGIARSVGLPDGKVVKHFSPPMIKLCEKHGIFPSVLAGENAIET
                     ALGEGTRFMMETDYIDDLERPGSVLGPKTVPRRTKQLIDKWGEDVFWKIHKENPEKVY
                     GVEINL"
     gene            complement(6752..9571)
                     /locus_tag="VKL21_07810"
     CDS             complement(6752..9571)
                     /locus_tag="VKL21_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HMB45711.1"
                     /translation="MARTKSKTELFGKHPLAKSIEPVIEKWSQENYPAVNGKQITHAT
                     RELMDYWFSDIAHEGEAFHICQRRAIETIIYCYEILSIPHVEALFEQFSCELLEKENL
                     RHGIESMEHPRFAIKMATGTGKTWVLTALIIWQYWNRTKLNDKRFASHFLLVAPGNIV
                     YERLLDSFLGKKRNGKRKPLTADLKKSLFMPENMRQDFDLRIFSKEDLQENMPFTESP
                     FILITNWHQLMDTSREKEITLAEDLGIQSRDNTVSERVERFMDFLTYNGDLMVINDEA
                     HHVHNASDAEQKRWQESIEELREKIKKNKDVLFTQLDFTATPFTIKGKKKEFFPHVVY
                     DYGLVEAMHAMLVKQLFIEKSSLLSNKIESLPESEKLMVTAHRDESNKPIELSEVQKH
                     MLDIGLAKLDALQGDFNKFKINKKPVMFVMADMNEEANMIANYIKQKIDSSGRSFGED
                     SRGEQVVTLHIGKKDSLSEDDFETLRNKVFSSDDASNPVRVIVSVLMLREGFDVKNVC
                     VLVVLRRSDSDLLTEQVIGRGIRQMFPEPEYRPEKIENYQKIKNKEPLINSYDLLFVV
                     EHPKYNEIYTQLTEAGAAIASGNSIDLSLDTKSVLINIDESRIKALDMSWPVSFAYKT
                     EEEINFSYFNVSELPFFGKPFEEIIQTRILITDFHPDTKYRREWELEESNFSYSVFLR
                     NTVKGIIGGNRRSAWLTRFAPDIIGIVDQYISGHLFGRKIDFNLDENLRKLRNQQVFD
                     FVVTEVRKKLMKFVQNAKSNEVIEADWTKLSTFKTLKVRMERVIATKKCVYPYVDFPP
                     RGGFERKFTQDVLENDSSVEAYVKLNQYVHQFSIPYINNMGHLVPYYPDFLVKTRDSM
                     LIIETKSTKDAANDIDTKIKAIAASGICSKISVVKNIPETNQPRIWNYVLLPQNILNE
                     MEGSGLQSLIERCEANLALLKMKRE"
     gene            complement(9578..>9935)
                     /locus_tag="VKL21_07815"
     CDS             complement(9578..>9935)
                     /locus_tag="VKL21_07815"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB45712.1"
                     /translation="RGCRQMHKICRIDINRSKELPGKIKSFVEKLTSRINIKKVYLFG
                     SVARNDFNEGSDIDIAIIGDFKERFLDRIDRILEMTDLPVEPLCYTEDEFEKMKRDDN
                     PFIKEILKGKVLFSSK"
BASE COUNT         2775 a         2066 c         1988 g         3106 t
ORIGIN      
        1 gagcttggcg cgtttgcatt gaaaggaaac gcccatcatg tcaaaatgaa cagttattgc
       61 attgtttttg gtatccagtc ccttgttaat tcacttgccg gtggatctac ccctgaagat
      121 gttgccgcag cagcatgcca cagtgtggct gagcaattat ttgagcagca attgcaggaa
      181 gtagatgtta aagaacctgt gatcatggta ggaggaactt cacttattca aggattgcca
      241 aaagcagtcg aagaattgct caaagtaaaa gtcatagttc ctgataacgc ccagtatatc
      301 ggagcggtgg gtgcagcttt gctggcatcg ggattcattt caaaatgaga ggatatgatg
      361 gaacaacctg agacatttat agttgaatcc cctgatgcta caggcgccag gaccacacgc
      421 agtctcatac tggatacggt gtcagaccta tcactttcca gggttatagt gagattgaag
      481 gtttttgtag atcctgttga acctgttttt attttagcgg cgcttcttcg tcttgggtcg
      541 ccttcaatca aacttaagga ttttgccaaa gtggatatgg gtgctcttgg caaagatgaa
      601 gtgaaaatag aactcgataa ggaattgttc gctgtaaaat tgttaaataa actctgggca
      661 aagtatggta agaacaatat tgagcagccg gacaggaaaa tcataattgt aaaaaccgat
      721 cctataaagg acctggatat gcttcgcgag cttgttatcg aagagcctca gcaggaagtg
      781 cttgaccggc ttattgatgc aatagcgctt cgcattatac ctgaaggatt cagggtcaga
      841 aaacatgagc tttcaaacac acatgtccta tttgtagcat cggaagatac gcttaaacct
      901 gaatggctgg caaaaggaaa agaaatagtg gattctttga ggagggaaga aaatgcttga
      961 accaatgatg tatataggcg gagcatataa acacagccag ctaatcgagc ttgttgaaga
     1021 tcttggcggt tacatcctgc aaaagaacat aatgcaatcg gaagtagtcc ttctgatgct
     1081 tgttcctatc aatgatctgg ctattgttga gacaaaatca aaagaacttc ttggaaaact
     1141 cacaagagcg cctcttacgg gcagcgaaat cgcaactgtg gctccaacac ttgcttacca
     1201 tcatcttcct catcttgatt gcgatgtcgc cgagttcctt cgaaaacagg gcgcaaagac
     1261 caacatggta ggtcttgccc ggggggttgg aaaaaggatt tgccagctta ctgcttttga
     1321 acgcgatctt atcaatgagc acgatgtcgc agtgttcata ctgggaaact acgaacattg
     1381 tatcatcgaa aaatcaaaga agctctatga agggattgaa atacctattg tggtcacagg
     1441 ggcgcctgaa ctcaggaccg aagatgtgcc caatgccaca gcatacgttg gaagtttcgg
     1501 taggatcctg catcatatga ggcacgctga ggaactcagg acgcttgata aactctccgc
     1561 ggtgatagga aaggtccttg atgatgtcag aatagagatc gccaaagatc cgccatctgt
     1621 ggcgccgccc cggatcaaga aagagataga ggagcaaatt ccggaaatcg ttgataacct
     1681 tcatcccctg cccataacat tgcaaatggg gggagtgagg gtaaagttgc cttatgaaga
     1741 ataccatgaa cagatagaga atatcaaatt cgtggaagga gtttctatga aagaagtggc
     1801 gcgcgttctg ccctcgaaaa tgaggaactt cattttaata aaggcgattc ccaagagcga
     1861 aacagggttt atgatatgaa caaatcgaat atgaaaataa gtgtcttttt tgcttataat
     1921 tagaagaaaa aattatatcg aatttgccac aggctcagtt catatcctcc atctcccatc
     1981 caccggaaag cattcattta tccacatcag tactttgttt tcatagatga tcttatactc
     2041 ccttgaaaga acaggcagtt ccccgaaaaa atccctgtga acaatccgga tattaagaat
     2101 atcgcgcctt gtctcaagct tgtgctccct caggattcgc cctatgggaa tatcggcccg
     2161 catgaggtca tcccttatac ctttgggtat cctgtttatc ggtgcaaggg attttgccag
     2221 aacataaacg aggtcatcaa ccttcaatgt gacaagacga tcatttacat catcacctat
     2281 ttcaatgtca agaagtccgg caatttgggg tgatgcttta acaacattct gggaaatcgt
     2341 ttcaactatt acactttttc cggtcagcac ttcaagaagc tgcgtgaccg aaccatcagt
     2401 tccggcacat attcgaagac aggcaggaat atccggtctc ttgaggacat cctgcaattt
     2461 caatctccct gaactgcttt ctttgcctgt ttgaggcgtt ctttggcagt gtagcctgtt
     2521 gtctcctcaa gaaatatcct gaaacgctta ttggtgtcaa gaacttcatt gtcttttatt
     2581 tccttatttg attctgaatc gacactcagt tttttatttt caattgagac ggttaaacga
     2641 ctaaaagcgc cataagttat cgttaatttt ccattttcct ctttgacctt ggccggaaaa
     2701 ttcttctcaa gcacgcttct ggttcgcccg atatcctggg aaaaacctct tttaaatgag
     2761 tactccttaa taatatcacc ttcacttcat aagcgtccac atatgattaa aagattactg
     2821 aaaaaggtta atcttatact taataaatat cttaaagggt taatgtttgc catgaaggat
     2881 atattttttt taaacgcgga atacgtatca tatcaccata taccgcccca tatttgcgct
     2941 taagctcatt atacttgtat tttaggaaaa tgctcaactc cggatgaatc gtatagctga
     3001 tatattcatc tgtgtaaata cttatcagat catcaaagtc ctttcgtgaa ttgccatgag
     3061 cataattctt tcccaaatac cgcctgatgt cgccattaaa taatggggaa acatgatgca
     3121 attcatgtat aaccgtcgac agtttggttt tttgattccc gtgattcagg aaacgcggca
     3181 aatgaaaata aacaatgtat agaatattta tgccatctat tatgatttcc ggcgctgcgt
     3241 attcaatatt ccggaccact tttgtctttg aacctccttc aaaaagcatg ggccgcagtt
     3301 ttgccacaac cccgttcata ttgccgtttg agggagatat cgcaacaagg atattctcaa
     3361 ttctaatatg tttgaaaaac ggatgcgttt taataatatc atccacaagc aaggccatat
     3421 tgcctgtgaa atcaaaacct ttaaccttat ttttgtctgt atttccttgc agcatccttt
     3481 aatgcctcaa taccgggaag tttttcaccg gccaggaaat caatacaggc gccccctccc
     3541 gtgcttacat gggaaaattt gtttgaaata cccatctgtt cggcagctgc tgcaatatgc
     3601 ccgcctccga taacagagaa acctgatttt gcccctgcct ttatgagttc ataagtcccc
     3661 agggcaaaag gttcattttc aaatacgcca gcaggcccgt tcatgaccac ggtttttgcg
     3721 ctgcttattt cccgggagaa tttaaccatc gtttctatgc cgatatcatg aataggcaat
     3781 tcaggcctga ggttactgat cttttcttcg atacgttcat cattcttatt caatgcaaca
     3841 tctacaggca gaccaatatt atccgggaaa cgtttgagaa gcttttttgc aatttcaatc
     3901 tggggtaagt agccctgctt atcgatgaaa ctcatattcg attccccgat atttaagcct
     3961 gaagctgcca ggaacacatt agccacgact cccgtaacaa gaaccctgtc agcacatccc
     4021 cgctcaagca cgttatttgt tactttaatg gaatcatcca cctttgttcc tccaagcaca
     4081 tagacgcacg ggcgctcgga gcaggacatt cccctattaa gggaatctat ctctttttcc
     4141 ataattcttc ccgctatgct tgttaatgtt tctgtaaagc ccgttactga aagatgcgac
     4201 ctgtgagaaa ctgaaaaagc atcgttcagg aatatatcgc aaacagaggc aagacgcctg
     4261 accatatgtg tttttttgtg gtcttttggt gcgcgttcaa gcgattcttc cgaatagaag
     4321 cgtgtatttt caagcaggat tatgtcgcct ttattcatat tcttaattgc ttcaatagca
     4381 tgggagccga aaatgtcatc aatatgattt atattgcgct tcattaattt agataattgc
     4441 ctcgcatgag gttccattgt agtaaaatcg cttttaccgg ggcggctctg gtgggaaagt
     4501 attacaactt ttgaatcttc caattccctt aaggttatga tgtggctcct gaatcggctg
     4561 tcatcaagaa tgatcccgtt agggtccatg ggcgagttaa ggtcaagcct gcacaagacc
     4621 gtcttgcttt ttatatcaat gtcatcgata gtcaggtaat ccctagtcat attgaagtcc
     4681 tttaattcta agaggatagc attataattt acggaaggat ttcataaagt agtagatttg
     4741 atcaataatt atagatagga atagtagata tataacaact attctatata tgtatatata
     4801 aacaagattc atattgctaa ttgcgcaatt tgaatgagcc aataacaaaa aaacgcaaga
     4861 atatgataaa gatatcttta agatacaagg tagcattgga actttatgcc agcctgtctc
     4921 tttttgctgc gatcataata attctttact acagcctttt tgacatgata ggagaaaatt
     4981 tatatgtttt atccatgggt attatcgggt tttttattat tgcattcatt tttttcctga
     5041 gaactcttat aacggatgtc aattctgtca aatttgaaaa tgataacagc aaagatgacc
     5101 cgaatattgt ggaaataagg ataatcaata aggtggcatt agaacttatt tcaagtattt
     5161 caaatattat aatacttgct gcaattttcg tattctggat cacaaaacca ggagggacaa
     5221 gttcagaagt cattttaatg ttgtttatca taattctttt gattcccatg ttgatattat
     5281 tgaaatcgct catttttgat ttcaatataa ttttcatgga taagaaacat cgagataatt
     5341 ttttgcatca attaataaat cgtaaaaacc ttgttttttt tgttattgcc ataataataa
     5401 ttttttcaat atttgcaagt aataaattaa ccacttcagt gggcaatcta tatcagaaat
     5461 ccatgtcaac gggtgaagaa accatcactg atatttcctc gaatttatat atcacatcta
     5521 ttgcggggga acgaaaaaat gtatccaaag gtcctattgt cggacttact gtatacattg
     5581 aggcaatgga tctggatttt aatttcaatt ccataacagt gaagtttata gacaaaacca
     5641 ctacatcaac attggaatac ggtatagagg ctgatgaatt gcacttttat tatgaaggaa
     5701 aaaagacggg aaaaggcaaa actgtactaa aaaagggtga catgggaatt attcaaataa
     5761 acttatcatc aacaaaacag gagctttatt ctttagacag gggaaaaatg cagttggttc
     5821 tgggtggtgg caaaactatt tcaagggatt tcagagtccc ggaattcaag ggagaatcca
     5881 ggatcttatt atattctaca gattgatctc caccccatac actttttccg gattttcttt
     5941 atggatcttc cagaatacat cctcgcccca tttatcaata agttgttttg tccttcttgg
     6001 gacggtcttt ggccccaaaa cggaacccgg tctttcaagg tcgtcgatgt aatcggtttc
     6061 catcataaaa cgtgttccct cacccagggc tgtttcaatg gcattctcac ccgcaagcac
     6121 gctcgggaaa atcccgtgtt tttcgcataa ttttatcatg ggcggtgaaa agtgttttac
     6181 tacttttcca tccggcaatc ccacgcttct tgcaatcccg actatttgtg caagcccttc
     6241 ctcggttgcg ctttctgtgt gaagctgcac tgcgcatcct gagtcccgtg caagctcaaa
     6301 gctgtagcgc atgatatcat ttgaatgatc ccaaattttg ggctcaactt cataatgagg
     6361 gcgcccggat ttcaatccaa ttgcttttcc ttcaactaca tattcgctgg caatttcaag
     6421 ccctcctctc attatttcca cagttcgcga aatgcccagg cgcccgtagt atttggtgag
     6481 ttcggcagga tggacgccaa gcacaacaaa gactttcact ttttcggctt cctcggcgcg
     6541 ccgggctatt ttgagtatcc tgtcaaacac cttcctataa tcctcaggcg agtttatttc
     6601 gattcccagc gaccagggcg gaagcgagac cagaacgata tgcgtgcccc ccgcgcgcgc
     6661 gaattcgcgg actgcgtcga ggcagcggcc ggaggggttg aggtgcatgt ggttgtcgag
     6721 gatggggagg gtggggggga tcatggttac tttattcccg tttcattttc agaagcgcaa
     6781 ggtttgcctc gcatctttca atcaaactct gcaaaccgct tccttccatt tcattaagaa
     6841 tattctgtgg taatagcaca taattccaga tgcgtggttg gtttgtttca ggaatatttt
     6901 ttactactga gattttggag catattcccg aagcagcaat tgcttttatt tttgtatcaa
     6961 tatcattggc ggcatctttt gttgattttg tctcaataat gagcatacta tctctggttt
     7021 ttacaaggaa atcaggataa taaggcacca ggtgccccat attattaatg taaggtatag
     7081 aaaattgatg cacatattga ttgagcttaa cataggcttc aaccgatgaa tcattctcaa
     7141 ggacatcttg tgtgaatttc cgttcaaatc ctcctctggg aggaaaatct acatatggat
     7201 acacacattt tttggtagcg attactcgtt ccatgcgaac tttaagagtt ttaaaagttg
     7261 aaagtttcgt ccaatctgcc tcaataactt catttgattt agcattctgg acgaatttca
     7321 ttaacttttt tctgacttca gttaccacaa aatcaaaaac ttgctgattt ctcaatttcc
     7381 tcaaattctc atccaggttg aaatcgattt ttcttccaaa tagatgtcct gaaatgtatt
     7441 gatcaactat gccaataata tcgggcgcaa aacgtgtaag ccaggcagat ctgcgatttc
     7501 caccgataat gcctttaaca gtgtttcgca ggaatacgct gtatgagaaa tttgattctt
     7561 caagttccca ttctcgtctg tattttgtat caggatggaa atctgtgatc aatattcggg
     7621 tctgtattat ttcttcaaaa ggtttcccaa aaaatggcag ctcagaaaca ttgaaatatg
     7681 aaaaattaat ttcttcttcc gttttatatg cgaatgaaac cggccagctc atatccagag
     7741 cttttatcct gctttcatcg atgttgatga gaacactctt tgtatcgaga gataaatcta
     7801 tggaattacc gcttgcgatc gcggcgccag cttcggttaa ctgtgtatat atctcgttat
     7861 acttggggtg ttcgacaacg aaaaggaggt cgtaagaatt gatgagaggc tccttgttct
     7921 tgattttctg ataattctct attttttcag gtctatattc aggttcaggg aacatctgcc
     7981 ttatgcctct gcctatcact tgctcggtca aaaggtctga atccgagcga cgaagtacca
     8041 cgagaacaca tacgttttta acatcgaatc cttcccgcag cataagaacg ctgactatta
     8101 ctctaacagg atttgaagca tcatcgcttg aaaatacttt attacgcagg gtttcaaaat
     8161 catcttccga gagtgaatct ttttttccga tatgcagcgt tactacttgc tcacctctgg
     8221 aatcttcgcc aaatgatctt ccacttgaat ctatcttttg cttgatataa tttgcaatca
     8281 tgttggcttc ttcgttcata tcagccataa cgaacatgac cggttttttg ttaattttga
     8341 atttgttgaa atcgccttgc agagcatcaa gtttcgccag tccgatatct aacatatgtt
     8401 tctggacttc tgaaagctca atgggtttat tgctttcgtc cctgtgggct gtgaccatca
     8461 atttttcaga ttcgggcagc gattctatct tatttgagag taggctgctt ttttctatga
     8521 aaagttgttt taccagcatt gcatgcatgg cttcaaccag accataatca tagacgacat
     8581 gcgggaaaaa ttcttttttc tttcctttga ttgtaaatgg cgttgcagta aaatcaagct
     8641 gtgtaaacag aacgtcttta ttctttttga ttttttcccg taattcctca atggattcct
     8701 gccagcgctt ttgctcagcg tcagaagcgt tatgcacatg atgcgcttca tcattaatga
     8761 ccataaggtc gccattgtat gtgagaaaat ccataaagcg ctcaacgcgc tctgaaaccg
     8821 tgttgtccct actctggata cctaaatctt ctgcaagagt aatttctttt tcccgtgagg
     8881 tatccatgag ttgatgccag ttggttatca ggataaaagg cgattctgta aaaggcatgt
     8941 tttcctgcaa atcttctttc gagaatatcc gcagatcaaa atcctgccgc atattttcgg
     9001 gcatgaacag tgattttttc aagtccgctg tcaaaggctt ccttttaccg ttgcgtttct
     9061 tgccaaggaa tgaatcaagc agtctttcat aaacgatatt tcccggagca acaagaagga
     9121 agtgggaagc gaatcttttg tcattaagct ttgttctgtt ccagtactgc catattatca
     9181 gggctgtcaa aacccatgtt tttcctgtac cggttgccat cttgatggca aacctcggat
     9241 gctccatgga ttcgatcccg tgacgcaagt tctctttttc aagtaattcg catgagaact
     9301 gctcaaataa tgcttccaca tgcggtatgc tgagtatctc gtaacagtag atgattgtct
     9361 ctatggcgcg tctctggcaa atgtggaaag cttctccttc atgtgctatg tctgagaacc
     9421 agtaatccat cagttctctt gttgcgtggg tgatttgctt tccattgact gcgggataat
     9481 tttcctgtga ccatttttct atgacaggct cgatagattt agccagcgga tgtttgccga
     9541 aaagttctgt tttggatttt gtgcgtgcca ttgaaaccta cttggaactg aacaggactt
     9601 ttcctttcag gatttccttg atgaagggat tatcgtctct tttcattttc tcaaattcat
     9661 cttctgtata gcacagaggt tctaccggca gatcggtcat ctcaagaatt ctatcgattc
     9721 tatccaggaa tcgctcctta aaatctccta ttatggctat atcaatatca ctgccttcat
     9781 taaaatcgtt gcgtgcaact gaaccaaaca gatatacttt tttaatattg atacgagaag
     9841 tgagtttctc aacaaaactt tttattttgc caggaagttc cttacttctg ttaatatcga
     9901 ttctgcatat tttatgcatt tgtctgcatc ctctc
//
LOCUS       DATGBN010000320         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_25008, whole genome shotgun sequence.
ACCESSION   DATGBN010000320 DATGBN010000000
VERSION     DATGBN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25008"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..390
                     /locus_tag="VKL21_07820"
     CDS             70..390
                     /locus_tag="VKL21_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45713.1"
                     /translation="MDDDFFVILNTVASMIVIICSVLSFQLLVGISRELKGLKTMDIM
                     WKNFIIMTNLFILLGITRAVGSIGTVIFEGFHPVFEALLSVTLILFFVFAIQLSISIE
                     EVTE"
     gene            391..963
                     /locus_tag="VKL21_07825"
     CDS             391..963
                     /locus_tag="VKL21_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMB45714.1"
                     /translation="MGKILSEEERRHMLEKLESKIVATRFMTLKYISSSISQDKVDFA
                     KMDMELPEFSKSLVRIIELLKEKDTEEMVKREAGVCLDNLKKKLNPALMQDVPLCTSC
                     GERVVVSYRFCTKCGVALNTQKWASTYKTCEKCQNIFDPKWNSCSYCGNQLIKKVEVN
                     KTCGFCKKNIEPSWLMCPYCGSKLKLVAGQ"
     gene            1186..1626
                     /locus_tag="VKL21_07830"
     CDS             1186..1626
                     /locus_tag="VKL21_07830"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HMB45715.1"
                     /translation="MATSTHSITITGKVQDIGFRNTIEHIGRSFGLPGMVFNAKDGSV
                     KILCSCEGAIDNFVQAIKERGAESGAVIVDFVDEVLPIDIDLPDNFAKASSDDEIDIG
                     RKLDKANVLLKTGFENLNTDLNTGFNKLNTDLNTGFNNHNDGRT"
BASE COUNT          642 a          313 c          404 g          552 t
ORIGIN      
        1 aaagatcgtc agtgcaatgc ggacaaagcc ctggaacgtt tttaccaagg atgaggagga
       61 gattgaataa tggatgatga tttctttgta atcctcaata cagttgcatc aatgatagta
      121 ataatctgca gtgtcctgtc atttcagtta cttgtgggta tttcaagaga attgaagggt
      181 ttgaaaacta tggatattat gtggaagaat tttataataa tgacaaatct tttcatatta
      241 ttaggtatca caagagctgt aggttctata gggactgtta tttttgaagg ctttcacccc
      301 gtatttgagg ctcttttatc cgtaactctc atattgttct ttgtattcgc gattcaactt
      361 tccatatcca tagaggaggt cactgaataa atgggcaaga tattatcaga agaagaaagg
      421 cggcatatgc ttgaaaagct ggagagcaaa atagttgcca cgcgttttat gaccttaaaa
      481 tatatttctt cttcaataag ccaggataaa gtggatttcg caaaaatgga tatggaatta
      541 ccagaattca gcaagagcct tgtaagaatt atcgaattac ttaaagagaa agacactgag
      601 gaaatggtta aacgtgaagc aggtgtttgc cttgataacc tgaagaaaaa actcaatcct
      661 gcattaatgc aggatgtgcc cttatgtacc tcatgtggtg aacgcgtggt tgtatcgtat
      721 cgtttctgta caaaatgcgg tgtcgcactt aatactcaaa aatgggcatc tacttataaa
      781 acctgcgaaa aatgccagaa tattttcgat cctaaatgga atagttgttc ctattgcggt
      841 aaccagttga tcaagaaagt cgaagtgaat aaaacctgtg ggttctgcaa gaaaaatatt
      901 gagccaagct ggttgatgtg cccgtattgt ggttccaaat tgaaacttgt tgccggccaa
      961 taatttttat atgggttttg ttatatgtat tatgcgttct accgtttctg ttatctgcaa
     1021 cggtctgcta aatagtatat ttataagcaa atcatttgaa aatccctcct tcccctaaat
     1081 tccttaattg taaaataatc aaaagagtat tctacacaca aacccgttac gttattgggt
     1141 atgtcataag atataaatac ttttaggcat tacattatat tctatatggc gacttcaact
     1201 catagcataa caatcactgg caaagttcag gatatcggtt tcaggaacac catagagcat
     1261 ataggacggt cgtttggtct gccgggtatg gtttttaatg caaaagatgg tagtgttaaa
     1321 attctttgtt cctgcgaggg cgcaatagat aatttcgttc aggcaataaa ggaacgcggt
     1381 gcagaaagcg gtgcagtaat tgtagatttc gtggatgagg tactgcctat tgatatcgac
     1441 ctgccagata attttgcaaa ggcatcctca gatgatgaga tcgatatcgg aagaaagctg
     1501 gataaagcta atgtattatt aaaaaccgga tttgagaact tgaacacgga cttaaacaca
     1561 gggttcaata aattaaatac ggacctaaac accggattca ataaccacaa tgatggcaga
     1621 acataacacc cgcctcgaag cgatccttga aaagctggcg gagaaataat gtttaaagat
     1681 acagtacagc cccgaaaaaa agtaactttt caagacataa tcggactgat aaaaacagaa
     1741 ggattacctg atgcagtgga acttaaaaag agagttcaaa gaggagaaaa attatgttcg
     1801 agcgcaccaa aaaaataaac ggccacgaat atagatatct cgtaaaatcg gtaagggtag
     1861 atgggaaaat gaagcagaag tttatcaagt atcttggaaa agttattcca g
//
LOCUS       DATGBN010000321         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_336199, whole genome shotgun sequence.
ACCESSION   DATGBN010000321 DATGBN010000000
VERSION     DATGBN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336199"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VKL21_07835"
     CDS             complement(<1..513)
                     /locus_tag="VKL21_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899149.1"
                     /note="FEN-1; Rad2; similar to eukaryotic enzymes;
                     endonuclease that cleave the 5'overhanging flap structure
                     that is generated by displacement synthesis when DNA
                     polymerase encounters the 5'end of a downstream Okazaki
                     fragment; as 5'endo-/exonuclease and
                     5'pseudo-Y-endonuclease activities; cleaves the junction
                     between single and double-stranded regions of flap DNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap structure-specific endonuclease"
                     /protein_id="HMB45716.1"
                     /translation="MGVDLGDLFERKEIELSDLKGKVIAIDAFNTLYQFLSIIRQRDG
                     TPLIDSHGEITSHLSGFLYRTTNLIEEGIKPVYVFDGIPPVFKNNTIEERQKIRAKAQ
                     EKWDEAKTRGDDKEAFKHAQASSRIKGNMIEDTKSLLEYMGIAVVQAPSEGEAQASSM
                     VKDGKSYAAGS"
     gene            complement(528..809)
                     /locus_tag="VKL21_07840"
     CDS             complement(528..809)
                     /locus_tag="VKL21_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45717.1"
                     /translation="MNLKIEPIGIIKKTASGLSDILIYSDFEHILGNVVEKFEKGTKV
                     LIIHKKIESSDQHQVQITSTELINRKGNLLTVKGIEADNDSVIDVRLGN"
     gene            1098..>1855
                     /locus_tag="VKL21_07845"
     CDS             1098..>1855
                     /locus_tag="VKL21_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMB45718.1"
                     /translation="MLDNYLFPGLVAFYLLGAVSALIFNKKDSLCSYISFFSASAGAL
                     SGMIFSLSVIFGNTFHLILNEGQFLEFGFFIDKLSAFFILVISISVFAVSIFSTGYVK
                     EYFGKKNIGHLCFLYNIFILSMILVISANNAIMFLIVWELMSVISFFLVVYEHEKIET
                     RKAGFIYIVMTHIGTGFILISFMIFAGTSGSFSFETFRAAGSTMTPFLKDIAFLFAFI
                     GFGTKAGIVPFHIWLPYAHPAAPGNVSALMSGVM"
BASE COUNT          484 a          365 c          333 g          673 t
ORIGIN      
        1 tgagcctgct gcataggatt tgccatcctt taccatcgat gaagcctgtg cttccccttc
       61 tgagggtgcc tggacgaccg cgatccccat atattcaagc aaactttttg tatcttctat
      121 catattcccc tttatccggg aagatgcctg agcatgttta aatgcttctt tatcgtcccc
      181 ccttgttttt gcctcatccc atttttcctg tgcctttgcc cttatttttt gtcgctcttc
      241 aatagtatta ttcttaaata cgggcggaat gccatcaaaa acatatacgg gcttaattcc
      301 ttcttcaata agatttgttg tcctgtaaag gaaacctgac agatgcgaag ttatctctcc
      361 gtgggaatca ataagtggcg taccatcgcg ctggcggatt atgctgagga attgataaag
      421 tgtattgaat gcgtctatgg ctattacttt tcctttcagg tcggaaagct ctatttcttt
      481 tctttcaaaa agatctccga ggtcaacccc catgataatc cttaaagcta attgccaagt
      541 ctcacatcga tcacagaatc gttatctgct tctatacctt ttacggtcaa aaggttgcct
      601 ttgcgattaa ttaattcagt tgatgtgatc tggacctgat gctggtccga ggattctatt
      661 ttcttatgta ttatcagcac tttagtacct ttttcaaatt tttccacgac atttccaaga
      721 atatgttcaa aatcagaata gattaatata tcagataatc cggaggcggt cttttttata
      781 ataccaatgg gttcgatctt aagattcaaa tggatgacct cttgaaattt attgttattc
      841 gcatgtcctg caatatattg ttattcgcat gtcctgcata tagctttttc tgaacaccag
      901 gattttttgt gtgaccgaaa tatggtatgg tggatatgat ccaacattaa taatcgatac
      961 atatcattca atttccggga aatcaccaga aaatcttaaa taccaaaaca gaggtgatac
     1021 gtgattctgt tgttcattaa aataataatg ataatttccg gtgttcatta aaatgataat
     1081 aataatttct ggtggttatg ttagataatt atttatttcc cgggttggtt gctttttatt
     1141 tgcttggggc agtatcagcg ctaatattta ataaaaaaga ctccctttgc tcatatattt
     1201 cgttttttag cgcatcagca ggcgcgcttt caggaatgat cttttcctta tcagtgattt
     1261 tcggtaatac atttcatctt attctcaacg aaggtcaatt cctggaattt gggtttttta
     1321 tcgataaact atcagctttt tttattcttg taatatcaat atcagtattt gccgtatcta
     1381 tattttcaac aggatatgtt aaagaatatt ttgggaagaa aaatattggc catctttgtt
     1441 tcctgtataa tattttcatt ctttcgatga tccttgttat ttctgcgaat aatgcgatca
     1501 tgttcctgat agtatgggaa ttaatgtccg tgatatcttt tttccttgta gtctacgagc
     1561 atgaaaaaat agaaacaaga aaagccggtt ttatatatat cgtcatgacc cacatcggaa
     1621 caggtttcat tctcatttcc tttatgattt ttgcaggcac atcaggaagt ttcagctttg
     1681 aaacattccg cgctgctggc tcgactatga caccgtttct taaagacatc gcattccttt
     1741 ttgcgttcat cggttttgga acaaaagcag gtatcgttcc ttttcatata tggcttcctt
     1801 acgcccaccc ggcggcgccc ggtaatgtct ctgcgctgat gtcaggtgta atgat
//
LOCUS       DATGBN010000322         6456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_258768, whole genome shotgun sequence.
ACCESSION   DATGBN010000322 DATGBN010000000
VERSION     DATGBN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258768"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VKL21_07850"
     CDS             <1..145
                     /locus_tag="VKL21_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305582.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HMB45719.1"
                     /translation="VKQAVEITLQLRGEAGRRQIEGANIGLTHNVGGSGGTAVVHIFG
                     RDK"
     gene            148..564
                     /locus_tag="VKL21_07855"
     CDS             148..564
                     /locus_tag="VKL21_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HMB45720.1"
                     /translation="MSVPRFWRRLKNLYNLIGTHCGKCGGYFYPPRNMCPECRRNGII
                     NPFKFKGAGEVVTYTIVHTSAKDYSKLTPYILAIIKLDEGPRLTSQVICDHSDIHIGM
                     RVKAAFRKLGEESDKGMIYYGTKFVPMESKNTRSGV"
     gene            926..1180
                     /locus_tag="VKL21_07860"
     CDS             926..1180
                     /locus_tag="VKL21_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45721.1"
                     /translation="MKKDNLQYILSVLTNDFETLATSEQVMKFKKKHCGVKWQNTLEK
                     DLLNYANNAFKLERWIGNVVTFMMEHNIHSNIQINNNNLK"
     gene            1537..2136
                     /locus_tag="VKL21_07865"
     CDS             1537..2136
                     /locus_tag="VKL21_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45722.1"
                     /translation="MKSWIIGILFVCIIVAGGIIYSSGFAADNSVPEGQGAGIAQTNT
                     EAQDMTSTTIDSLETASSTDGKVSVTALTCTIINSGDYVVDLRLKNPGIEARTITIDP
                     SGTKIDLLPDQIKRFDLHLGTEASILALLVDDGTELQVQSPPCTNSGSSGSGGGVSGL
                     SNPTKQKFSPPPPVPELSPLVLTLAGISGLLLVSRIRKN"
     gene            complement(2505..2894)
                     /locus_tag="VKL21_07870"
     CDS             complement(2505..2894)
                     /locus_tag="VKL21_07870"
                     /inference="COORDINATES: protein motif:HMM:NF016229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HMB45723.1"
                     /translation="MRHIVLSGISHHVLRDLEHEKIKTIEIRSPHNFLSALETNVGDL
                     IFLTATSQEDMRPGTSGLIVRIREKQVSMHRVIQKTPESYEESEFQLARLQLEMRGRG
                     RVRRAECCKLGEATLVDADEVLFFEGR"
     gene            complement(2899..3642)
                     /locus_tag="VKL21_07875"
     CDS             complement(2899..3642)
                     /locus_tag="VKL21_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HMB45724.1"
                     /translation="MASETSEQFVKIINEPLKSKNPLIIEGFPGIGLVGNITCQHIIE
                     ELGMKYMGSIDSKYFPPLAVLFNGIVYMPVRIYEAPEKEIIVIISDIPIHPSASYDIS
                     KVLIDWMQTINARNIVSIAGIATTTGERRVFGGATSAELLEKIKDKTEIFQVGTISGI
                     SGSIMTECYLRGLPAISLLGETPGPNPDPRAAVQVITVLNKIYDLAIDTEKLLGQADQ
                     IELELSKLADQVKTTESSALPRKEFPMYG"
     gene            3752..5206
                     /locus_tag="VKL21_07880"
     CDS             3752..5206
                     /locus_tag="VKL21_07880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HMB45725.1"
                     /translation="METGEIIQIFAESGFQIDPQALDAMRTSSHKQIQHVVKTMDSSV
                     LVVGIEHICPLVQKPLQKHISLPLPEIPIKKDPMPIKIKILESPVTILKDITNQSTCI
                     GEYTEFVQYFRDRYSSLGEILRKRLGARPIESLKKRNLDTREPLSVIGMVLDIKTTSN
                     GHKLIQIEDTTGTLRILIHKERDKELFDSANHILLDEVIGITGTLSSDGNLMFVNRIS
                     WPDIPNSRKVTLDGKKAVGKAVFISDIHVGSKTFLEDSWLKFIDWLGDEINYLVIAGD
                     VVDGIGIFPGHDKELAISDIYEQYEKAAQYLNAVPKHIKIIISPGNHDAVRQAEPQPR
                     FPERITKMFRNDIIFVGNPAMVDIGVKVLIYHGRSMDDIIASIPGFSYTEPNKPMNEM
                     LKLRHLSPIYGGRVSIAPEKKDHFVIDEIPDILHCGHVHTVGVSRYRDVLTINSGTWQ
                     TQTEFQKRMNLMPVPARATLVDLSGMEYKIMDFN"
     gene            5264..5998
                     /locus_tag="VKL21_07885"
     CDS             5264..5998
                     /locus_tag="VKL21_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HMB45726.1"
                     /translation="MIWKSGIQSWDDFLINHGMLKATKKKQRLLYSGVEESIEQLSGK
                     NHVFFARRLLPCHHWRAYRQFEEKTAFIDIETTGLSSQSDSITIIGIYNGKETKTYIR
                     GVNLHEATEELAKYKQLVSFNGARFDLPFIEHEFPGFFNHLHIDLMYPLKNIGYSGGL
                     KKIEYSLGIRRSEETEGISGLDAVRLWNKYKKGNDEALEVLIKYNKEDVENLKTIIEM
                     TYQKMVDNEVAPKDRKCANVVSNKTI"
     gene            complement(5981..>6456)
                     /locus_tag="VKL21_07890"
     CDS             complement(5981..>6456)
                     /locus_tag="VKL21_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMB45727.1"
                     /translation="VGTRIIIILLSVILLIAALRIFFFDNIGFMVKDGENKKILIGAG
                     LAFIFGIISSLVGIGGGTFIVPLLLIIGLEVKNAAATSAFIITFTSFSGFAGHLLFGT
                     QTLDIRVLFYAGLAAFAGAQIGSKIIFKRVSSKGISNLFALVLLFVAGKLLYGLI"
BASE COUNT         2081 a         1268 c         1319 g         1788 t
ORIGIN      
        1 tgtgaaacag gctgttgaaa taacacttca actgcgcggc gaagctggca gaaggcagat
       61 agagggtgcc aatataggtc ttacgcataa tgttggaggc tcaggcggca ctgctgttgt
      121 tcatattttc gggagggata aataagcatg tcagtcccaa gattttggag aagacttaaa
      181 aacctttata atctcatcgg aacgcactgt ggaaaatgcg gcggttattt ctatccgccc
      241 aggaacatgt gccccgaatg caggaggaac ggtataataa atccttttaa attcaagggg
      301 gccggcgagg tggtaacgta tactatcgtg catacttccg caaaggatta ttcaaagctt
      361 acaccatata tacttgccat aattaaactg gatgaaggcc caaggcttac atcccaggta
      421 atttgcgatc attcggatat ccatattggc atgagggtca aagcagcatt ccgcaagctt
      481 ggtgaagaga gcgataaagg aatgatatat tacgggacaa aatttgtccc gatggaatct
      541 aagaacacaa gatcaggtgt ctgacatact ctctgccctg aagtgcagag gttctctgac
      601 ccgtacaaac ctcatgtggt tgttacctat cagtctgcac atcaatggtt ctgtcagtga
      661 gacgctacta cttacgaatg taggtattcc attcgaccgt agataccaag tatttcttat
      721 gaaccaagtt gcatcatttt ctgtttgaat catgcccgtt ggttgttgtg tacgagataa
      781 tacttatctt gcttggtgtt ttgaaatgat tagttttagt atgcgctcac cccttaaccc
      841 cacgattctt tcaaacacaa ctatcagatt ttgtgtatga atatatcgat aaatttaaat
      901 attatcataa tcagtatcat tatttatgaa gaaagataac ctgcaatata ttttaagtgt
      961 cttgacaaat gactttgaaa ctctcgcaac ctcagaacag gtaatgaaat ttaagaaaaa
     1021 acattgtgga gtgaaatggc agaatactct ggaaaaagac ctcttaaatt atgcgaacaa
     1081 tgcatttaaa ttggaacggt ggattggaaa tgtggtaaca tttatgatgg agcataatat
     1141 ccattcgaat atacagataa ataataataa cctaaagtaa tacataaaaa aaaaagaaaa
     1201 catccaatct attttaacac aatttccgaa ttgggtttca ggatagaaac taaaacagta
     1261 ttcaaaataa tcgataaaca tataagttgt ttacggggag tctggccagg catgtgaact
     1321 ccgatttcct cctgtaatgt tgaatttaaa ccagatcatt gctaaggggg gacaggcaca
     1381 tcattgtatt attggggcat ggttttaaaa aattcatata taaactatga tgagtatgat
     1441 gataattatt tcataattaa aattttaaaa cagtaaatta tatatgtttg tcattcaata
     1501 atgagttact atgtcaaaac aaaggtgata taaaatatga aatcttggat aatcggaata
     1561 ttatttgtgt gtataattgt cgccggcgga atcatttatt cttcgggatt tgctgctgac
     1621 aatagcgttc ctgaaggaca aggggctgga attgcacaaa ccaacactga agcccaggat
     1681 atgacttcca cgactattga ttcacttgaa acagcatctt ccacagatgg aaaagtatct
     1741 gtgactgcgc tgacatgtac aatcataaac tcgggagact atgtagttga ccttagacta
     1801 aaaaatccag gcatagaagc cagaacaatt acaattgatc catccggaac aaaaatagat
     1861 ttgttaccag accagataaa aagatttgac cttcatcttg gaacggaagc ttctatactc
     1921 gctctcttag tggatgatgg aacagaactt caagtccaat cgcccccctg tacaaactca
     1981 ggcagttcgg gaagtggcgg tggagtaagt ggcctttcca atcccacaaa gcagaagttc
     2041 tccccgcctc ctccagtccc ggaactttct ccattggtat tgacattggc tggaatttcc
     2101 ggactgttgt tagtatcgag gataaggaaa aattaagaaa agaataaaca gaactgtaat
     2161 tcaaaaggaa taaaaggaca aagagaaata tcctgcactg ttttgcatgg gtatgtctcc
     2221 tttccatttt ttgcgttttt ttaatattat aacaccaaaa gattttactc ccactgcatt
     2281 cacatagtct tatataccag gttaaaaaga atattgatta tgaatccgcc gtttgcagat
     2341 ttgttcctca aaaaaatcag gagaacattt tgggagaaaa tgttatgaat tcaggcgacc
     2401 gctataagta tatgtccata cgacaattac aggacctttc gaacgatttg aaagaagaag
     2461 caaaaagtga cccatcacgt gacaaacaga ttttgaagag aaaactatct tccttcaaag
     2521 aacaggactt catcggcatc aaccagcgtg gcttctccaa gtttgcagca ttcagcacgg
     2581 cgaacccgcc ctcgccctct catttccaat tggaggcggg caagctggaa ctccgattcc
     2641 tcataggact caggtgtctt ctggataacc ctgtgcattg aaacctgctt ttcacgaatt
     2701 cgtacaatga ggcccgatgt acctggtctc atgtcttcct ggcttgttgc tgtaagaaat
     2761 ataaggtctc ctacattcgt ttccagcgca gaaaggaaat tatgtgggct tctgatctcg
     2821 atggtcttga tcttttcatg ttcaagatca cgcaggacat gatgtgatat tccactcaat
     2881 actatatggc gcatgagatc acccgtacat agggaattct ttcctgggca atgcagacga
     2941 ttcagtagtc ttgacctgat ctgcaagttt tgacagttca agttctatct ggtcagcctg
     3001 gcccaacaat ttctcggtat caatggccag gtcatatatt ttattaagaa cagttattac
     3061 ctggaccgct gctctcggat caggattggg gccaggcgtt tcacccagaa gacttattgc
     3121 aggcaagcca cgaagataac attctgtcat tatgcttccg gaaatacccg aaatagtccc
     3181 tacctggaat atttcagtct tatcttttat tttctcaagc aattctgcac ttgtcgcgcc
     3241 cccgaaaaca cgtctttctc ctgttgttgt agctattcca gctattgaaa ctatgttcct
     3301 ggcatttatt gtctgcatcc agtctatcag gaccttactg atatcatatg atgcggaggg
     3361 atgtatgggt atatcggaaa ttattactat tatctctttt tcaggagcct cataaatgcg
     3421 aacaggcata tagacaatcc cgttaaatag aacagccagt ggagggaagt atttggaatc
     3481 tatagagccc atatatttca ttccgagttc ttcaatgata tgctggcagg ttatgttccc
     3541 tacaagacct atgcccggaa acccttcgat aattaatgga ttctttgatt taagaggttc
     3601 attgatgatt ttcacaaatt gttctgatgt ttctgatgcc atgcttccac cttactagga
     3661 ttgacccgaa tatacatata tctaatgcaa ggatcaagga aaatcttatc ccatttcgaa
     3721 gccatataga ttaaaatatg gagacaggta gatggagact ggcgagataa tccagatttt
     3781 tgcggaatcc ggattccaga tagatccgca ggcgcttgac gccatgagaa caagctccca
     3841 taaacaaata caacatgttg ttaagacaat ggacagttca gttctcgtgg tgggaattga
     3901 gcatatatgc cctcttgtac agaaaccact acaaaagcac atatccttgc ctttacctga
     3961 aataccaata aagaaagacc ccatgccgat aaagatcaag atattagaaa gtccggtcac
     4021 aatacttaaa gatattacaa accagtccac ctgcatcggg gaatataccg aatttgtgca
     4081 gtatttcagg gaccgctaca gttccctggg tgaaatcctt cgaaaaagac ttggcgcaag
     4141 acctattgaa agccttaaaa agagaaatct tgacacaagg gagcctttgt ctgtgatagg
     4201 tatggtactg gatataaaaa caacctcaaa tggccataaa cttatccaga tagaggacac
     4261 aacaggaaca ttacggatcc tgatacataa agaaagagat aaagaactgt ttgactcggc
     4321 aaaccatatt ctgcttgatg aggttattgg aataaccggt acactatctt ccgatggcaa
     4381 tcttatgttt gtgaacagga tatcatggcc tgacattccc aattcccgga aagtcaccct
     4441 ggatgggaaa aaagcagtgg gaaaagcagt gttcatttcc gatattcatg ttggcagtaa
     4501 gacttttctt gaagattcct ggcttaagtt catcgactgg ctcggggatg aaataaatta
     4561 tcttgttata gcaggtgatg tggttgatgg gataggtatt tttccgggtc atgataagga
     4621 acttgccatt tccgatatat atgaacaata tgaaaaagca gcccagtatt tgaacgcagt
     4681 cccaaaacat atcaagatca ttatttcacc tggaaaccat gacgctgtga gacaagctga
     4741 gccgcagccc cgttttcctg agagaattac caaaatgttt cgcaatgata ttatctttgt
     4801 aggaaatccc gccatggtgg atatcggggt aaaagtcctt atctatcatg gaagatcaat
     4861 ggatgatatt attgcaagca tacccggatt ttcatatacg gaaccaaata aaccaatgaa
     4921 tgaaatgtta aaactgaggc acctttcacc gatctatgga ggaagggtat ctatagcgcc
     4981 ggaaaaaaaa gatcatttcg tgatcgatga aattcctgac atccttcatt gcggccatgt
     5041 tcatactgtt ggcgtttcac gctacaggga tgtacttaca atcaacagcg gtacatggca
     5101 gacccagacc gaattccaga aaagaatgaa tcttatgcct gtgccggcaa gagctactct
     5161 tgttgacctg tccggaatgg aatacaaaat aatggatttt aattgagata tgttgaacaa
     5221 tacctatatt catataccct gtatcggctc ttcaacagaa cggatgatat ggaaatccgg
     5281 gatacaaagc tgggatgatt ttctcataaa tcacggaatg ttaaaagcga ccaaaaaaaa
     5341 gcagcggtta ttatattcag gagttgaaga atcaattgaa cagctctcag gcaaaaacca
     5401 cgtttttttt gcacgtcggc tcttgccctg tcatcattgg agggcataca ggcaatttga
     5461 agaaaaaacg gcatttatag atatagagac tacgggttta tcttcacaat ccgacagcat
     5521 aacgataata ggtatatata acgggaaaga aacaaaaaca tatattcggg gcgtcaacct
     5581 tcatgaggca acagaagaac tggcaaagta caagcagctg gtttccttca atggtgcgcg
     5641 ttttgatctt ccttttatcg aacatgaatt tccgggattt ttcaatcacc tgcacatcga
     5701 cctgatgtat cctttaaaaa atatcggata tagcgggggt cttaagaaaa tcgaatattc
     5761 actcgggata agaagaagcg aagaaacaga gggcatatcc ggtcttgatg ccgtgcgttt
     5821 gtggaataaa tataaaaagg ggaatgatga agccctggaa gtgcttatca agtacaacaa
     5881 agaagatgtt gaaaatctaa agactattat tgaaatgact tatcagaaaa tggtggacaa
     5941 cgaggttgca ccaaaagaca gaaagtgtgc taatgttgta tcaaataaga ccatatagaa
     6001 gttttcctgc cacaaataac aaaacgagag caaataaatt gctgatacct ttggatgaaa
     6061 cgcgcttgaa aattattttt gaacctatct gcgcgcctgc aaaagcagct aatcctgcat
     6121 aaaataatac tcttatatcc agcgtctggg ttccaaaaag gagatgcccc gcaaacccgg
     6181 aaaatgaagt aaatgttata ataaatgcag aggttgcagc agcgtttttt acctccaatc
     6241 cgataatcag aagaagaggt actataaaag tgcctccgcc aatacctacc agagaggaaa
     6301 ttattccaaa aataaaggcc aggcctgccc cgatcaaaat cttcttattt tctccatctt
     6361 tgaccatgaa gccaatattg tcaaaaaaaa atatacgcaa agctgcgatc aaaagtatga
     6421 ctgaaagcag tatgattata atcctggttc ctaccc
//
LOCUS       DATGBN010000323         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_219665, whole genome shotgun sequence.
ACCESSION   DATGBN010000323 DATGBN010000000
VERSION     DATGBN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219665"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1125
                     /locus_tag="VKL21_07895"
     CDS             115..1125
                     /locus_tag="VKL21_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB45728.1"
                     /translation="MKIIDILIVFVIIALASGCLGQKATETPPVETPISSTGIDSTGG
                     PEYTIAYLSDGRPGKEKSNSERNLTIDFNQIIPQSPTKRVDAVIMLGDMDTIEKTSRA
                     YAESTARNIPVFYEIGNHELENKADLPQIKKLFASYPFNPLPGPDGSKDTTYSFDAGD
                     MHIVVLNEYWDGNNNGVCDWKTPSGGINADDSCFKYNDADGGFIPDALLKWLENDLDR
                     NTKNWTIVTGHEPLYPGRRHVGDSLDKDKENRDKLEKLFFSKNVSAFIGAHTHGSGVI
                     TKDNIFHVDAGVCGIQTLLGSGDSFASIIYTTVDSNGHFKIIHTRENPTWVTPKNDIY
                     SK"
     gene            complement(1133..>1955)
                     /gene="asd"
                     /locus_tag="VKL21_07900"
     CDS             complement(1133..>1955)
                     /gene="asd"
                     /locus_tag="VKL21_07900"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMB45729.1"
                     /translation="DPKKVDADIVFSALPAELAKTMEPEFAKAGFVVASNASALRMMK
                     DVPLVIPEVNPDHLGLIDVQQDERKWDGYVITNPNCTTIMMAVTLKPLMKFNIEKIYI
                     ASMQAISGAGYDGIPGMAILDNVIPYIGQEEEKVETETKKLLGEFNGSEIIDAPFKVS
                     ASCNRVMVIDGHTEAIWAEMADDPSPGEVKQAFLDFDPGLSGLPTEPTPVIEVKEEKD
                     RPQPRLDRNIGGGMTVSVGRIRPGIRYICMGHNTIRGAAGASVLNAELLKKKGKL"
BASE COUNT          595 a          400 c          400 g          560 t
ORIGIN      
        1 caatagttgg cgatattgcc gagaaacaag cccaaatata agaataacag atttttttat
       61 tttttccaaa gattatgtag agtattccac atagtcggaa tttaaataaa aaatatgaaa
      121 atcattgata ttttaattgt atttgtgata attgcattag cttcaggttg tttaggtcaa
      181 aaggccactg aaacacctcc tgttgagaca cctatatctt ccacaggtat tgatagtaca
      241 ggcggtcctg aatacacaat agcatatcta tcggatggaa gaccaggcaa agaaaaatca
      301 aacagtgaaa ggaatctgac aatagatttc aatcagataa ttccacaatc cccaacaaaa
      361 cgagtggatg ccgtaataat gcttggagat atggatacta tcgaaaaaac ctccagagct
      421 tatgcagaat caacggcaag aaatattcct gtgttctatg agataggaaa ccatgaattg
      481 gagaacaaag ctgacctgcc ccaaataaaa aaattatttg catcctaccc ctttaatcct
      541 ttgcctggac ctgatggttc aaaagatacg acctattcgt tcgatgcagg agatatgcat
      601 attgttgtat tgaatgaata ctgggatgga aataacaatg gagtatgcga ctggaaaact
      661 ccatccggcg ggatcaatgc agatgattca tgctttaaat acaatgatgc tgatggcgga
      721 ttcattccgg atgcactttt gaaatggtta gaaaatgacc ttgaccgaaa tacaaagaac
      781 tggactattg ttacaggtca tgagccatta tatccaggta gaagacatgt gggggactca
      841 cttgataaag acaaagagaa tcgcgataaa cttgaaaaat tattcttctc caaaaatgta
      901 tcggctttta ttggcgcaca cactcatggt tcaggtgtga tcacaaaaga caatattttt
      961 cacgtagatg ccggagtttg tggaattcaa acattattgg gctcgggtga ttcgtttgcg
     1021 tccattattt atactacagt agattctaac ggtcatttca aaataataca caccagagag
     1081 aatcccacat gggtgacgcc aaaaaatgat atttatagca aataattgca ggctataatt
     1141 tccctttctt tttcaggagc tctgcattca gtacacttgc tcccgctgcg cctcttatgg
     1201 tattatggcc catgcagatg tatcgtatgc cagggcggat acgtcccacc gaaacggtca
     1261 ttccgcctcc gatgttccta tccaggcgcg gctgaggtct gtctttttct tcttttacct
     1321 cgatcacagg tgtaggttcg gttggcaatc cggataatcc aggatcaaaa tcaaggaaag
     1381 cctgttttac ttcaccaggt gaaggatcgt ctgccatttc tgcccagatg gcctcagtat
     1441 ggccgtctat gaccatgaca cgattgcagc ttgcgcttac cttaaaaggt gcatcaatta
     1501 tctcgctgcc gttaaattca ccaagaagct tttttgtttc agtttcaact ttttcttctt
     1561 cctggccgat atagggaata acattgtcaa gaattgccat tcctggaatg ccatcataac
     1621 cagcgcctga gatagcctgc atggatgcta tatatatttt ttcaatattg aatttcatga
     1681 gcggtttcag ggtaacagcc atcatgatag tcgtgcaatt gggattcgtg ataacatagc
     1741 cgtcccattt gcgttcatcc tgctggacgt caataagacc gagatggtcc ggattaactt
     1801 ccgggattac aagaggaaca tctttcatca tacgcaaagc gctggcatta cttgcaacta
     1861 caaaacccgc ttttgcaaat tcaggttcca tggtttttgc aagttcagcg ggaagagcag
     1921 aaaaaacaat atccgcatca actttcttgg ggtca
//
LOCUS       DATGBN010000324         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_375286, whole genome shotgun sequence.
ACCESSION   DATGBN010000324 DATGBN010000000
VERSION     DATGBN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375286"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..395
                     /locus_tag="VKL21_07905"
     CDS             42..395
                     /locus_tag="VKL21_07905"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB45730.1"
                     /translation="MNENFTKTKAMNRNIKKIVNQISTILVAGIDARQVYLFGSHAHN
                     TANNDSDIDIFIVADLYGKKKIEITQQARRLLLKKIFMPVDILVCDINDFNNRKDNLS
                     TFEYSIATEGIKIYG"
     gene            388..636
                     /locus_tag="VKL21_07910"
     CDS             388..636
                     /locus_tag="VKL21_07910"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMB45731.1"
                     /translation="MDNKEGLKWFEYAENDFEAAKILSLQVKPKLEIVCYHCQQCAEK
                     MLKGYIASKNGKLQKTHVVKPVKITIPILKKSYYIVVI"
     gene            974..>1588
                     /locus_tag="VKL21_07915"
     CDS             974..>1588
                     /locus_tag="VKL21_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HMB45732.1"
                     /translation="MSPNTRKKVFVSGCFDILHSGHIAFLKEASQFGDLNVGLGSDKT
                     VADLKGRKTINSEDERLHMLEALSCVHNVKVNRGSGILDFLDDMKALKPDIFIVNEDG
                     HTPDKEQLCKDLCIEYKVLRRIPYANLPVRSTTSLRKETPIPYRIDLAGTWIDQPYVS
                     KYNPGWAITASIEPTIEFNERSGMATSTRKKAIELWNDHLPMENP"
BASE COUNT          549 a          277 c          300 g          462 t
ORIGIN      
        1 attcgtattt tcagtacatt tgttaatcaa aatattttgt tttgaatgaa aacttcacaa
       61 agactaaagc aatgaaccga aatatcaaaa aaatagtaaa tcaaatttca accatattgg
      121 ttgctggtat cgatgccaga caggtttatc tttttggttc acatgctcat aatactgcaa
      181 ataacgatag cgatattgat atatttattg tagcggatct atatggcaaa aagaaaattg
      241 aaataaccca acaagccagg aggttgcttc taaaaaaaat atttatgcct gtcgatattt
      301 tggtatgcga catcaatgat tttaacaata gaaaggacaa tttatccaca ttcgaatatt
      361 caattgcaac agaaggaata aaaatatatg gataataaag aaggactaaa atggtttgaa
      421 tatgccgaaa acgactttga agctgcaaag atcctatctc tccaggtaaa gcccaagctt
      481 gaaattgttt gttatcattg ccagcaatgt gccgaaaaaa tgctgaaggg ttatattgct
      541 tcaaaaaacg ggaaattgca aaaaacgcat gttgtgaagc ctgtaaaaat tacgatcccg
      601 attttgaaaa aatcatatta cattgtagtg atctgacaat atattcttct gaagtaaggt
      661 atccaaacat tcttgaaatc gaatattatc acatgcaaaa tgccataagt gatgccaatc
      721 aaattaagga atttgttttt agcaaattaa ttgattttga acaattgcaa tagcataaat
      781 gatccgccag tttcaagaca gttatttcga cagatgttct caatcatcag tgtggggtta
      841 tgttaaccac caacgctcaa ataaggttat atttgaaata atcaagaacc gggcgggatt
      901 tggcttcgcc gagctccact atgttttcac ttttgtcttc ttttaacaca gtaaacacat
      961 agaaattcat aatatgtccc cgaacactcg taagaaagtc ttcgttagcg gttgtttcga
     1021 tatcctccat tccgggcaca tagcattctt gaaagaagcc tctcagtttg gtgatttgaa
     1081 tgtcggattg gggtctgata aaacggttgc tgacctgaaa ggacggaaga caataaattc
     1141 tgaggatgaa cgactacata tgcttgaagc gttgtcttgt gtgcataatg tgaaagttaa
     1201 cagaggcagc gggattcttg attttctgga cgatatgaag gctcttaaac cagatatttt
     1261 cattgtaaac gaggatggtc atacacccga caaagaacaa ttatgcaaag atctttgtat
     1321 tgaatacaag gttctgcgaa gaatccctta tgccaatttg ccggtacgtt ccactacctc
     1381 tttaagaaaa gagaccccaa taccatatag gattgacctg gcaggaacat ggattgatca
     1441 accctatgta tcaaaatata atcccggctg ggcaataacc gcatcgattg aaccaacgat
     1501 tgagtttaat gaacggtcag ggatggctac ctctaccagg aaaaaagcaa ttgaactttg
     1561 gaatgaccat ttaccaatgg aaaatccg
//
LOCUS       DATGBN010000325         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_219772, whole genome shotgun sequence.
ACCESSION   DATGBN010000325 DATGBN010000000
VERSION     DATGBN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219772"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..341)
                     /locus_tag="VKL21_07920"
     CDS             complement(153..341)
                     /locus_tag="VKL21_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45733.1"
                     /translation="MSKFGENADLILEMLHNGKKVDLDEIVLPSDAAIIDFMREFDLI
                     ELNEGTIRITKSGLELLG"
     gene            604..1512
                     /locus_tag="VKL21_07925"
     CDS             604..1512
                     /locus_tag="VKL21_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMB45734.1"
                     /translation="MVLKKIAELLRLCNTPASIISPFPFFTIAIFLISQNNFNYYGIH
                     FLFIGIVVTLLSNFASNLWNHCNDMKEDIAQGKKNILTQDPSMQKIAVFASILLYVIS
                     IIIVYQISLEVKRPIYIYFLIWAIATWCYSDNLILKKLTGFRLKEHYIGELVTYGIAW
                     PMYTLSIWLIYSDITIICEIILAAFLFFSISLLLLKDLKDISGDRKAGLRTFGVVFSP
                     SKLIQYSCYLMIIYYLILLNPMTVNLFSNRILIMIVPFAYFLKNTFLHMYYKNWSLDI
                     GDFHAIKSVGYSVYGSVIFLGLSTLL"
     gene            complement(1509..2798)
                     /locus_tag="VKL21_07930"
     CDS             complement(1509..2798)
                     /locus_tag="VKL21_07930"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HMB45735.1"
                     /translation="MDFKPDLILFYATLKYNGNYQSMLDVFHEEYGDIPQIGASVDGM
                     IYPDDMRTDGSALVLCKDEGARISVKGLNEKGVINSAEKLAKMVKCDNGSIVLHFPLV
                     RVPSVLKSAEFFAKGYYYSKQCNGTTFEKKKSYVRKFAEYCDKENIFYLPPTILEIFA
                     KQTNYRVPIIGVNVMHTHVRLNSPSIFCNFKDIGGGIAALIIEKQGVNAIFDDIFPEK
                     GKTMEETRRNVRDEFTIIKEFKANFEKNILISLDGMAPVEAVENLIYAAEKEKEEMLG
                     KLEKGDYKLYIPYGLSFFNKKTNGVFLLGIGSYYPFELFPFYIDVSDYSDDVALVYEI
                     IEEKFEAFISFLNNLKYNDRFVYFFLDIGVATAFGDKVFEYKDKVKQLLGENYFGVLS
                     CAPSAYIPLEFQMRKYLSECDENIFFSSAGTNACLEI"
     gene            3099..4556
                     /locus_tag="VKL21_07935"
     CDS             3099..4556
                     /locus_tag="VKL21_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45736.1"
                     /translation="MKRSNTPSFITELPLKTTTADEATILVRLDTGRQLYHACLGEAL
                     KLLAYIQQSKEYQEIQKLPKTTNGEQNKERTKAFNELNQKYGFTDYDIQHYATGIRNS
                     WMEEHIHAHIAQKLATRAFKAVQKKAFGTAKNVRFKGKNQMDSLEGKEADTGLRFENN
                     SLLWSGLEIPCIIDPENKVIAYGLSHKVKYCRIVRRKINGKNRFYLQLINEGTPYQNP
                     KNKIGPEEIGVDVGVGTIATVADTKATLKQFCSELIPDQKKKRKLQRKLDRSLRATNP
                     DNYNKNGTVKKGKKFNKSNHYKTISKDLAETDRILAAHRKSLHGQDINEIIATGTRIQ
                     TEDVSYKGWQKRYGRSINMRAPSMLMDSLKRKAENAGGYFFEIPTQTTKLSQTCHICE
                     QAVKKTLSKRWQICCGIRMQRDLYSAFLAKCVDKETSALDFARAKDLWQGMESIFSEA
                     ISRAKETAIGKVCPASFGFTGIQSIAVRSLILYEL"
BASE COUNT         1559 a          848 c          846 g         1413 t
ORIGIN      
        1 tgccaaaagc aaaacaaatg accttgcaat atgaagcgcc aatacagaaa gcgcaagaaa
       61 tgatgttaca acaaaatata ctgatagaaa ggattagaga aataacgata caatatgaaa
      121 acctccatta agagggacta aataaattgg atttaaccca gcagttctaa acctgacttc
      181 gtaatcctga tagttccttc attcaattcg attaaatcga attcacgcat aaaatcaata
      241 attgctgcat ctgaaggcag tactatttca tcaagatcta ctttctttcc attatggagc
      301 atttcaagaa tcaaatctgc gttttcccca aattttgaca tactattaga taataattgc
      361 tttcttatat aataccacgg aacaaagttc cttaactctt tttcttaata taatttctaa
      421 actatattta ctgttagttt atattatccc tggtcattgt agtaattgcc ccaaaagggt
      481 taaaatgaag gagacattta aaatggaaaa cacaaaaatt gagaaccaga gaaaagatga
      541 cgaagaaaaa gtagcagagc aaatttttaa gaaagctgct cttggtttcg cttagaatca
      601 aaaatggtgc tgaaaaaaat agctgagttg ttaagacttt gtaacactcc ggcttcaatc
      661 atttcaccat ttccattctt tacgatagca atttttttaa tatcgcaaaa taattttaac
      721 tattatggta ttcattttct ctttattgga attgtcgtaa cacttttatc aaatttcgct
      781 tctaatctat ggaaccactg taatgacatg aaggaagata tcgctcaagg gaaaaaaaat
      841 attttaacgc aagatccttc aatgcagaaa atagctgttt ttgcttcgat tttgctgtat
      901 gtaatttcta taattattgt ttatcaaata tcattggaag ttaaaagacc gatttatata
      961 tatttcttaa tatgggccat agcaacctgg tgctattcgg ataatttaat tcttaaaaaa
     1021 ttgactggct ttagattaaa agagcattat ataggggagt tggtaacata tgggattgca
     1081 tggccaatgt atactcttag tatctggtta atatattcgg atataactat tatttgtgag
     1141 attattttag cagctttttt attttttagt atatcgttat tattattgaa agatttaaag
     1201 gatatatccg gtgacagaaa agcgggtttg aggacttttg gggtggtttt ctccccctca
     1261 aaattgattc aatattcatg ttatttgatg ataatttatt atcttatatt attgaatcca
     1321 atgactgtaa atctttttag taatagaata ttaattatga tcgtcccatt tgcatatttc
     1381 ttaaaaaata cttttttgca catgtattat aagaattggt ctttggatat cggagatttt
     1441 catgcaatta aatcagtggg ttattccgtg tatgggtcag taattttttt agggcttagc
     1501 acattgcttt aaatttctaa acaggcatta gtgccagcgc ttgaaaagaa aatattttca
     1561 tcacattccg ataaatattt cctcatctgg aattctaagg gaatatatgc ggagggagca
     1621 catgatagta ccccaaaata gttttctccc agcaattgtt ttactttatc tttatattca
     1681 aaaactttat ctccaaaagc tgttgcgaca ccaatatcca ggaagaaata aacaaaccga
     1741 tcattatatt ttaaattatt taaaaatgat ataaaagctt cgaacttctc ttcaattatt
     1801 tcatatacaa gagcaacatc atccgagtaa tccgaaacat caatataaaa gggaaataat
     1861 tcaaacggat agtatgaccc tattccaagc aggaaaactc cattagtttt tttattaaaa
     1921 aatgaaagtc catatggtat atatagctta taatctcctt tttcaagttt ccctaacatt
     1981 tcttcttttt ctttctcagc tgcataaatt agattttcca cggcctctac tggagccata
     2041 ccatccagag aaattaaaat attcttttca aaattagctt taaattcttt gattattgtg
     2101 aattcatctc tgacattgcg tctggtttct tccattgtct ttcctttttc cggaaagata
     2161 tcatcaaaaa tcgcattcac accttgtttc tcaattatta gagctgcgat cccccctcca
     2221 atatccttga aattgcagaa tatgctggga gaattgagcc tcacatgcgt atgcataaca
     2281 tttacgccaa ttattggcac gcgataattt gtttgctttg caaaaatttc caggattgtg
     2341 gggggcaggt aaaaaatatt ttctttatcg cagtattcgg cgaattttct tacataactt
     2401 ttcttttttt caaaggttgt tccattacac tgtttgctgt aataatatcc ttttgcaaaa
     2461 aactcagctg atttgagtac acttggtact ctaacgagag gaaaatgtaa cacaatagat
     2521 ccattgtcgc attttaccat ttttgcaagt ttttcagccg aatttatgac tcctttttcg
     2581 tttagacctt tgacacttat ccgggcgccc tcatctttgc ataacacaag agccgaccca
     2641 tcggtgcgca tgtcatcagg ataaatcata ccatccacag aggcgccaat ttgaggtata
     2701 tcaccatact cctcatggaa tacatccagc atggattggt aattgccgtt atatttcaag
     2761 gtggcataga ataatatgag gtccggctta aaatccattt tcgaacgcgc ctgttcaaca
     2821 aggtctttag cagcttgatt atggtctttc ttattgctga aaaatgttat tgacttcgtt
     2881 ttaccacctg tcttaatttt ctatatgctt cttttggata aatatcttat catcaaaatt
     2941 atgatgatga aagtatgaca gaatcgtggg gttaacaaat catgaaccga ttggttcata
     3001 catactgtat gaaaaatttc tttttttcat acgaaggggt gaacgcaagc ctccggcttc
     3061 agccgtgggg atagcgaacc ccttagaatt gtaattgcat gaaaagatca aacactccat
     3121 cgttcataac agaactcccc ttaaaaacca caacggcaga cgaagcgaca atacttgtac
     3181 gtctggatac tggcagacag ttgtatcatg cctgtcttgg ggaagcactc aaacttcttg
     3241 catatatcca gcaatccaaa gagtatcagg aaatccagaa actcccgaaa acaacaaacg
     3301 gtgagcagaa taaggaacga acaaaagcat tcaacgaact caatcaaaaa tacggattca
     3361 ctgattacga tattcaacat tatgcaactg gaataagaaa tagttggatg gaagaacata
     3421 tccacgctca tatcgctcag aaattagcaa cgagagcatt caaagcagtc cagaaaaagg
     3481 catttggaac agcaaagaat gtacgattca aaggaaaaaa ccagatggat tctctggaag
     3541 gaaaagaggc tgatactggc ttgagatttg agaacaatag cctcttatgg tctggtcttg
     3601 aaatcccatg tatcatcgat ccagagaaca aagtaatcgc atacggctta agccataaag
     3661 ttaagtattg tcgaatcgtg cgaagaaaga ttaatggcaa gaaccgattt tatcttcaat
     3721 taattaatga aggaacacca tatcagaacc cgaaaaacaa gatcgggcca gaagagatcg
     3781 gggtagatgt tggggtaggt acaatagcga cagttgcaga tacaaaagct actctgaaac
     3841 agttttgcag tgagttaatc ccggatcaga aaaagaaacg aaagctgcaa cggaaactgg
     3901 ataggtcact tcgggcaacc aatcctgata attataacaa gaacggaacg gtcaagaagg
     3961 gaaagaagtt caacaagtcg aatcattata aaacaataag caaagacttg gctgaaacag
     4021 atcgtatctt agctgcacat cgaaaatccc ttcatggtca agacataaac gaaataatag
     4081 caacaggaac acgaatccag actgaagacg tatcctacaa aggctggcaa aagcgatacg
     4141 gcaggtcaat caatatgcgt gctccatcaa tgttgatgga tagtctcaaa cgaaaagctg
     4201 aaaatgctgg tggctatttc tttgaaattc caacacagac aacaaaacta tcacaaacat
     4261 gccatatctg cgaacaggct gttaagaaaa cccttagcaa gcgatggcag atctgctgtg
     4321 gtatcaggat gcagagggac ttgtattctg catttctggc aaaatgtgtg gataaagaaa
     4381 caagcgccct tgattttgcc agagcgaaag acctctggca aggtatggaa tccatcttta
     4441 gcgaagcgat ttcaagggct aaagaaactg cgataggcaa ggtttgtcct gccagctttg
     4501 gattcacggg aattcagagc atagcggttc gctcgttaat tctgtacgaa ttataagtga
     4561 gactatggat gctgtaatcc tcccggatga gagccataga gaacttatat gaacgcgcgg
     4621 aatctcccgg ctttagccgg ggagaggttc aacatggggc taagag
//
LOCUS       DATGBN010000326         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_219828, whole genome shotgun sequence.
ACCESSION   DATGBN010000326 DATGBN010000000
VERSION     DATGBN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219828"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VKL21_07940"
     CDS             <1..191
                     /locus_tag="VKL21_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45737.1"
                     /translation="PKDLSVRVHSCPSCGLVLKRDHVSAMIIESRSSTAGIAGIQAHK
                     SNLNRETMKREAPLFKVE"
     gene            294..785
                     /locus_tag="VKL21_07945"
     CDS             294..785
                     /locus_tag="VKL21_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45738.1"
                     /translation="MIEGVPAKKTTVYEEKAPYGNTIKLVMMSGLFALAFLYFIAVFR
                     KPEQAEITLLLIVSISALVTWSFFNMKFRITTDGVEAAMPPFKYRIPFSEIKEVKTIE
                     DIPWYAGWGMRIWGRRLAFISMRKRAVLIEKEKGFFRKLILTTQNPEEFIKKLKEGMN
                     TFQ"
     gene            1129..1341
                     /locus_tag="VKL21_07950"
     CDS             1129..1341
                     /locus_tag="VKL21_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45739.1"
                     /translation="MNTTTMLYVGIDVSKDKSDICLVMLYETIESIKRRTPGKSDVVF
                     GMEATGVDQKFPAKKQRKYTFMQEIY"
     gene            1365..1694
                     /locus_tag="VKL21_07955"
     CDS             1365..1694
                     /locus_tag="VKL21_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45740.1"
                     /translation="MTVEIKTLIRQLELYEEQINSVEIKIEQSMNRINSKIMSIPGIG
                     DTLGPIILGEIGNVDRFSSVKKLIAFAGIDPVVSQSGRFQSMSAKILRIVYWVLKNNK
                     EYQTQLN"
     gene            complement(2117..2338)
                     /locus_tag="VKL21_07960"
     CDS             complement(2117..2338)
                     /locus_tag="VKL21_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45741.1"
                     /translation="MTEEDKGYTEIKMSSGWFMTISMQKSDKFEEEKEYVEIAKERSG
                     QKRGRFNINPKYVRALGEALVKFADDNKL"
     gene            complement(2335..>2505)
                     /locus_tag="VKL21_07965"
     CDS             complement(2335..>2505)
                     /locus_tag="VKL21_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HMB45742.1"
                     /translation="VAPLAMINIRTEPIVAVGAIISDIPLVDNVEQNPVDVIKNGDKV
                     LVDAILGSVEIL"
BASE COUNT          811 a          473 c          508 g          713 t
ORIGIN      
        1 tgccaaaaga cctgtcagtg agggtacatt catgtccttc atgtggtctt gtgttaaaaa
       61 gagaccatgt atctgcgatg ataatagaaa gtagaagtag tacggcaggt attgccggaa
      121 ttcaagccca taagagtaat ctcaatagag aaacgatgaa gcgggaagct ccccttttca
      181 aagtggagta gttcacaagc catacttata taaacgagtc actataatat attatccaga
      241 cgtataattg taaggataaa ataaatgaaa aagaaaatgt actcggcacg cgcatgatag
      301 aaggagtgcc tgcaaagaaa actacggtct atgaggaaaa agcgccttac ggcaatacga
      361 ttaagctagt aatgatgtct ggcttattcg cactggcttt tttgtacttt atagctgtct
      421 ttagaaagcc tgaacaggct gaaatcactt tgcttttgat agtatcaatc tcagctctgg
      481 ttacttggag ctttttcaac atgaagttca ggattacgac cgatggtgtg gaggctgcaa
      541 tgcctccttt taaatacaga atcccgttct ctgaaatcaa agaggtaaaa acgatagaag
      601 acattccctg gtatgcaggt tgggggatgc gcatctgggg aagaagactt gctttcatca
      661 gcatgcgtaa aagagcggta ttgattgaaa aggagaaagg gtttttcagg aaacttatac
      721 taaccactca aaatcctgaa gaattcatta aaaaattaaa agagggaatg aataccttcc
      781 aataaatccc gccataggcg gcgcctcact ggggtctggg gtcgcaaccc cagcgccgtt
      841 aagataatgt ggaccaaaag ttttcgtata tacaacattc ttgcgagaat aaataaataa
      901 tacttgcacc tacataaaag taggtcgaga gggtaacaaa aattgtgttt gggatccgtt
      961 cccgtctatt aacctgttgt cctctcatcc ttttaataca cgaataagta cgttttggac
     1021 atatataaat gttccacata ttactcgcga acttgtgtac gaaaggatgg gggagggaca
     1081 tgactctttc gatctaacca ataaagcaaa atcaatgagg tcgataaaat gaataccaca
     1141 acaatgttat atgtcggaat agacgtttca aaagacaaat ccgatatctg cttggtgatg
     1201 ttatatgaaa caattgaaag tataaaaaga agaacaccag gaaagagcga tgttgtcttc
     1261 ggaatggaag ctaccggagt cgatcaaaaa tttcccgcca aaaagcagag gaaatatacg
     1321 ttcatgcagg aaatatattg actatacccg aactcgattc gataatgact gttgaaatta
     1381 aaacacttat cagacaactt gaattatatg aagaacagat aaattcagtt gagattaaaa
     1441 tagaacaatc aatgaaccgg attaattcaa aaataatgtc aatacctggt attggagata
     1501 cattgggtcc tatcattctt ggggaaattg gaaatgtcga caggttttca agtgttaaga
     1561 aattgatcgc ttttgctgga attgatcctg ttgtgagcca atctggaaga tttcagagta
     1621 tgtctgctaa aatattgaga atagtttact gggttttgaa gaataacaag gagtatcaga
     1681 cacaactgaa ttaagtttag gatagtaaaa ggggaactgg cctgacaaat gaatagtgga
     1741 tactattttg tttgatcgtg caagtaatga gtgttgttat tatatgcaga cggtgatttc
     1801 atacttaacc ccgccagcgg cggcgcaacc gttggggtct ggggtcgcaa ccccagcgct
     1861 ataaagctaa tgttgaacga aagttttaat agtacgctaa tagaacgctt atctgcggtt
     1921 ccttttctca gaaaccccat agcaatcaat aacgaaccat aaccacaaat ccagccacag
     1981 ttttatgttt tgcaatacaa atgccaaaat caggttgcca tttattttga tatttattta
     2041 aaccgcagat gaacgctgat aaatgcagat atattgcacc ggatttatta atcgcagatt
     2101 tatcgatcgt tatttttcaa agtttgttat catccgcgaa cttaacaagt gcttcgccca
     2161 gcgcacgcac atatttggga ttaatattga accttccccg cttctggccg cttctttcct
     2221 tggctatctc cacatattct ttttcttcct cgaacttgtc gcttttctgc atcgagatag
     2281 tcatgaacca gcccgaactc atctttattt cagtgtaacc tttatcttct tcagtcataa
     2341 aatttcaaca cttcctaata tcgcatccac taaaacctta tcgccatttt ttattacatc
     2401 aacaggattt tgttcaacat tatcaacaag cgggatatcc gagattatgg cccctactgc
     2461 cacaattggc tcagtacgga tatttatcat agccagggga gccac
//
LOCUS       DATGBN010000327         5255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_530526, whole genome shotgun sequence.
ACCESSION   DATGBN010000327 DATGBN010000000
VERSION     DATGBN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530526"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VKL21_07970"
     CDS             <1..515
                     /locus_tag="VKL21_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45743.1"
                     /translation="TVRLSYAMSIDGLFPGIFSRIHPRYGTPYMALLIHGIIAFTLSI
                     FSGIPKLISFSVFNLAFSFILTCLALIILKKKSEKNLYGQNILPWIGIGICLYLLYFT
                     SVLDKVVGSALILFGILLYVFFSPKADIHHLKALFTSEENIFMRTMETKERFLANFIR
                     LLHKAYRKVK"
     gene            615..1223
                     /locus_tag="VKL21_07975"
     CDS             615..1223
                     /locus_tag="VKL21_07975"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMB45744.1"
                     /translation="MAISNMLFVSKQTPQGPSQMDLYSRLLDEQIIFLNGEINDDMAE
                     SIIAQLLYLEAQEFEGDIRIYINSPGGVITAGLAIYDTMQCIKCNVATICVGQADSMA
                     AILLASGTKGKRMAFPNARVMIHQPLGGAEGQASDIQIQAKEMNRAKNLLDDILVYHT
                     GQPIEVIEKDTDRDFFLTAEKALEYGLIDKIIKAGEGVVKVK"
     gene            1234..3018
                     /locus_tag="VKL21_07980"
     CDS             1234..3018
                     /locus_tag="VKL21_07980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HMB45745.1"
                     /translation="MIDKLGSEDVIWDLTSLYKDIGDDNITKDIEYIKSKITTFSTKY
                     RKKLNVISPGEFLDAIIALEEINQKTSRIGSFAYLNFTTQSNNAQAGAFLQRFEEISS
                     QFQKELLFFELEWASLEDEAANPLLENLALGKYRHYLEKLRKYRPHQLIETEEKILAE
                     ISPVGAGNWNKLFEKVLSTIKFGEAGRTEEEVLSDLYDPDRMIRIKSAKEFTEGLESQ
                     KHILTHIFNTILADKMIMDRLRRFPDWVSSMNLDNEIDEKIVTALIEAVKNRYDIPQR
                     YYRLKKELLGYDELFDYDRYCPLPFSPEKKITWDQGKEMVLKAYKDFSPEMMDIALKF
                     FDGRWIHAPIMPGKRGGAFSDPVTPDVHPYVMINYSGINRDVQTLAHELGHGVHQYLA
                     GKKQGYLNSRTPLIMAETASVFGEMLVFRSQLELAEGRNEKLSLLCNKLEEIFATVFR
                     QIAMNRFEDDIHRERRKLGELSTDRFGELWIDTQKEMFGDSVTLTRDYSRWWSYVTHF
                     LEAPGYVYAYAFGELLVLSLYKMFMEEGKSFVPRYLNLLASGGNGSPAQLLADFGVRL
                     DDPGFWNEGLDIIDKMLKEAEGLAEQGR"
     gene            3021..4409
                     /locus_tag="VKL21_07985"
     CDS             3021..4409
                     /locus_tag="VKL21_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMB45746.1"
                     /translation="MSFESLKSGKRIVVIGGVAAGMSAASKARKLISDIEIFVFEKSG
                     QISYGACGLPNLISDLIKSPEDLVSHDFKFFKEERDINVFLYHEVVAIYPAKQSVLVK
                     NLQTGAENEYQYDKLVITTGARAFVPQIKGIEMKGIFTIKTLQDGIETKDFIRKNVPG
                     RALIIGAGHIGMEMAEALAMAGMDVTITEREPDILGTMDDEITKIIEEGLVRNRVKLF
                     KSASVREFISKNGYVREAILDNSESINADIVIVCIGMRPNSEIAKEAGIETDRTGAIK
                     VNQRMETNIPGIYSAGDCAMAYHQVLERQVYIPLGTTANKQGKVAGENMAGGNASFKG
                     IVGTSVLKVFDLEVARTGLSETDAQIEGFNFISNVVEQSSRAHYYPGGSKICVKLIAG
                     MNTGRILGAEMVGRESVSKRIGIFATAIAAKMTVEDIGDLDLSYAPPFAPVWDPILIA
                     ANDLKEKLNEAK"
     gene            4592..5002
                     /locus_tag="VKL21_07990"
     CDS             4592..5002
                     /locus_tag="VKL21_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMB45747.1"
                     /translation="MGKMVSINPATGEVNGEFELYSASRINIAIKKAGTAFIEWKNLD
                     VSERAEYLKNAAKVLRRRKNELGKIITMEMGKVIKESVPEIEKCALAFEYFAQNTGGF
                     LEPEVAETDAKKAYVEFVPIGVYEFMNIRSISVV"
     gene            5004..5225
                     /locus_tag="VKL21_07995"
                     /pseudo
     CDS             5004..5225
                     /locus_tag="VKL21_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
BASE COUNT         1665 a          985 c         1231 g         1374 t
ORIGIN      
        1 ctactgtacg attatcgtat gctatgtcta ttgacggttt atttcccggg atcttttcta
       61 gaattcaccc cagatatggc acaccatata tggcattatt gatccatgga ataattgcat
      121 ttacattatc tattttttct ggaataccta aattgatctc attttctgtt tttaatcttg
      181 cattttcatt tatattaacc tgtctcgcac tgatcatact gaaaaaaaaa tcagaaaaaa
      241 atttatatgg tcaaaatatt ttaccatgga tcggaatcgg aatatgctta tatctccttt
      301 attttacatc tgtgttagat aaggtagttg gttcagcgtt aatattattc ggtatactcc
      361 tgtatgtgtt tttttctcca aaagcagata tacatcatct aaaagcatta tttacttccg
      421 aagaaaatat ttttatgaga acgatggaaa cgaaagagcg gttcttggca aattttataa
      481 gattgctgca caaagcctac cgaaaagtga aataaataaa tattctcttt atatttcaag
      541 ctatttactg cttaagctta taccgatgga tttttttata ttcagagcaa tactctattt
      601 acgaggaaag aacaatggca atttcaaaca tgcttttcgt atctaagcaa acaccacagg
      661 gtccgagcca gatggaccta tactctcgcc tgctggatga acagataata tttctcaatg
      721 gtgaaatcaa tgatgacatg gcggaaagta ttatcgccca acttctatat ttagaagcac
      781 aagagttcga aggcgacata cgcatctata taaatagccc tggaggagtg attactgccg
      841 gtcttgctat ctatgatacg atgcagtgta ttaagtgcaa tgttgcaact atttgtgttg
      901 gacaagctga ctcgatggct gcgatcttac tggcatctgg aacgaaggga aagagaatgg
      961 catttcctaa tgcacgagtg atgatacacc agcctcttgg cggtgcggag ggacaggcat
     1021 cggatatcca gatccaggca aaagaaatga acagggccaa gaaccttctg gatgatatcc
     1081 tcgtctatca tacagggcaa cccattgaag tcattgagaa agatacagac agagacttct
     1141 tccttacggc agagaaagca ctggagtatg ggttgataga taaaataata aaggcgggag
     1201 aaggggtagt aaaagtaaaa taaaaataat atcatgatag acaaattggg cagtgaggac
     1261 gtgatatggg atttaacctc cctttataag gatatcggtg atgacaatat aacaaaagac
     1321 atcgagtaca ttaaaagtaa aatcaccacg ttttcaacaa aatatcgaaa gaaactaaat
     1381 gtcatatcac caggtgaatt tctcgatgct atcatcgcac ttgaagaaat aaaccagaaa
     1441 acctcacgta tcggctcgtt cgcatacctt aattttacga cacaatcgaa caatgcgcaa
     1501 gctggcgcat tcctccagag gttcgaggag atttcaagcc agttccagaa agaactgctc
     1561 ttctttgagc tggaatgggc ttccctggaa gatgaggcgg caaacccgct ccttgaaaat
     1621 ctggcccttg gcaaatacag gcattatctg gaaaagctca ggaaatacag gccgcaccag
     1681 ttaatcgaaa ccgaagaaaa aatccttgct gaaatatcgc cggttggagc tggaaactgg
     1741 aataaactct ttgaaaaagt actctccacg ataaagttcg gggaagcggg caggaccgaa
     1801 gaggaagtcc tgtcagacct ttatgatcct gaccgcatga taagaataaa gtcagcaaag
     1861 gagtttacgg aaggactgga atcccagaaa cacatcctga cacatatttt caatacgata
     1921 ctggcagaca agatgataat ggataggctg cgcagatttc cggactgggt aagttccatg
     1981 aacctcgaca acgagataga tgaaaaaatt gtaaccgcgc ttattgaagc tgtcaaaaac
     2041 cgatatgaca tcccccagag atattacagg ctaaaaaaag aactgcttgg atacgatgaa
     2101 ctattcgatt atgacaggta ctgtccactg cctttctccc cggaaaagaa gataacctgg
     2161 gatcagggaa aggagatggt tcttaaggct tacaaagatt tttccccgga gatgatggat
     2221 attgccctga agttctttga tggcagatgg atacacgcgc ccattatgcc gggcaaaagg
     2281 ggaggagctt tctcagaccc cgtaacacct gatgttcacc cgtatgtgat gataaactat
     2341 tctggaataa atcgtgatgt ccagaccctg gcacacgagc ttggacacgg cgtccatcag
     2401 tatctggcag gaaagaagca gggttacctc aacagcagga caccactaat aatggctgaa
     2461 acagcatctg tttttggaga aatgcttgtg ttcaggtccc agcttgaact ggcagaagga
     2521 aggaatgaaa aattgagtct gctgtgcaat aaactggaag agatctttgc cacggtgttc
     2581 aggcagatag ccatgaaccg tttcgaggat gatatccata gagagcggcg caagttaggt
     2641 gagctgtcaa cagaccggtt tggcgaactt tggatcgata cccagaaaga aatgttcggg
     2701 gattctgtga cacttacccg ggattattcg agatggtgga gctatgttac acatttcctg
     2761 gaggctcctg ggtatgtata tgcctatgcg tttggtgaac tcctcgtgct atctctttat
     2821 aaaatgttca tggaagaagg taagagcttc gtccccaggt atttgaattt gctcgcttct
     2881 ggcgggaatg gctcacctgc acaactgctt gcagatttcg gagtaaggct tgacgacccc
     2941 ggtttctgga atgagggatt ggatatcatt gacaaaatgc taaaagaagc cgaaggattg
     3001 gcagagcaag ggagataaga atgtcgttcg aatcgctaaa atctggtaag cgtattgtgg
     3061 taattggggg cgttgctgcc gggatgagtg cagcgagtaa agcacgaaaa ctgatatctg
     3121 atattgagat atttgtattt gaaaaaagtg gtcagatttc ctatggcgcc tgcggcttgc
     3181 cgaaccttat atcggattta attaaatctc cagaagatct tgttagccat gatttcaaat
     3241 tctttaaaga agaaagagac atcaatgtat ttctctatca tgaagtagtt gcaatatacc
     3301 cggcaaaaca atctgtactt gttaaaaacc tccagacagg agcagagaac gaatatcaat
     3361 atgataagct cgtgatcaca acaggtgcaa gggcatttgt gccacagatc aaagggattg
     3421 agatgaaagg aattttcacg ataaaaacac ttcaagatgg aatcgaaaca aaagatttta
     3481 tcaggaaaaa tgttcccgga agagctctca tcattggtgc cggccatatc gggatggaga
     3541 tggcagaagc acttgcaatg gcaggaatgg atgtcacaat aactgaaaga gagcctgata
     3601 ttttgggaac tatggatgac gaaattacca aaataataga ggaaggactt gtaagaaaca
     3661 gagtcaagct ctttaaatcc gcatctgtga gggaattcat cagtaaaaat ggttatgtca
     3721 gagaagcgat tttagacaac agtgagtcaa tcaatgctga tattgtaatt gtatgcattg
     3781 gtatgaggcc aaattccgag attgcaaagg aggctggtat tgaaaccgac cgcacaggag
     3841 caatcaaagt caatcaacgg atggagacga atattcccgg catctattca gccggggatt
     3901 gtgcaatggc gtatcatcaa gtcctggaaa ggcaagttta cattcccctt ggaaccactg
     3961 ctaacaagca agggaaagta gcaggagaaa atatggcagg gggaaatgct tcattcaagg
     4021 ggatcgtcgg aacttccgtc ttaaaggtct ttgacctcga agttgcaagg acaggtcttt
     4081 ccgaaacaga tgcgcaaata gaaggtttca attttatctc caatgtggtt gagcagagtt
     4141 caagagctca ttattatcct ggtggcagta agatatgtgt caagctaatt gcgggaatga
     4201 acactggccg aattctcgga gccgaaatgg tcggcaggga gagtgtttcg aagcgaatag
     4261 gtatttttgc aactgctatt gcggctaaaa tgactgtgga agacataggg gacctcgatt
     4321 tgagttatgc gcctccattt gcacctgtat gggatccaat tttgattgct gcaaatgacc
     4381 ttaaagagaa attaaatgaa gcgaaataat accttcacgc gtaaggtata tcgtagaccc
     4441 gtctgcgctt cctgagatgt gagccggaat ctgtcgttcc attttttcag attccataac
     4501 ttttatttca tcaccaacta ttttatatga tatcaggcgc aaatcagtaa ttaatataaa
     4561 agacatgggg ttcgtcaaac aaggtgaaga tatgggaaag atggtctcta ttaaccctgc
     4621 cacgggagag gtgaacgggg aatttgaact ttattccgca tccaggataa acattgcgat
     4681 taaaaaagct ggaactgcct ttatagaatg gaaaaatctg gacgtatccg agagggcgga
     4741 atatctcaaa aatgctgcaa aagtcctcag aagaagaaaa aatgaacttg gcaagatcat
     4801 taccatggaa atgggtaagg ttatcaaaga gtcagtacct gagattgaaa aatgtgcatt
     4861 ggcgtttgaa tattttgccc agaatacagg gggattttta gaacccgaag tcgcagaaac
     4921 ggatgcaaag aaagcgtatg tcgaatttgt acctatcggg gtatacgaat tcatgaacat
     4981 tagatccata tcggtggttt gaaatgaagg gcagcgatct ttttataaaa tgtcttgaga
     5041 atgagggcgt aacgcatata ttcggcgtac caggagaaga aatactggac attctggact
     5101 ccctttcgag gtcaaagata aaattcattg ttacacggca tgagcatggg gcggccttca
     5161 tcgcggatgt atatggcagg ctttcgcaca ggccaggggt atgcctttcc actctcgggc
     5221 caggctgcac ttgaaaaaac tcacaaagag gctca
//
LOCUS       DATGBN010000328         5001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_453152, whole genome shotgun sequence.
ACCESSION   DATGBN010000328 DATGBN010000000
VERSION     DATGBN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453152"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VKL21_08000"
     CDS             <1..221
                     /locus_tag="VKL21_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45748.1"
                     /translation="SLSGRNCTYVIQQGIPENAPEGEIRKDKAFTRDELEALALSISF
                     LKDVRIRTKERGEEKICGIESPLKDMAK"
     gene            182..1051
                     /locus_tag="VKL21_08005"
     CDS             182..1051
                     /locus_tag="VKL21_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMB45749.1"
                     /translation="MWNRESFKGYGEIITAAVLWSLAGIFAKQIHGMSAQSIIFYRVV
                     FAFAIFFIFILISKNFRIIQLKDKKIYLLLFGILQVGTMLTFFISLLKGSVSIAVLLL
                     YTAPVYITILSPWLLKERSTKKGIFALILSILGILFIVDPAKLDLAHYPAGILAGIAS
                     GIIYAFQIMTSKYASSTYSGYSQAFWSFPVAIMILLPVGTAPFNVVMDNLVYLILLSI
                     FPTILAVSLYFNGLKKVNTHSASIVGLIEPLSAVILSVLFLHEQISILEIIGGALILA
                     GVSLVTIDEPLYS"
     gene            1032..1880
                     /gene="map"
                     /locus_tag="VKL21_08010"
     CDS             1032..1880
                     /gene="map"
                     /locus_tag="VKL21_08010"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HMB45750.1"
                     /translation="MNHYIHDYYLEAGRIAAKVRTETVSMIKEDIPLLEIAEFVEERI
                     KDLGANPAFPCNISINEIASHYTPQDYLPCFRKGDVVKLDLGTHIEGYIADTAATLEI
                     GTNHHHKLIRTCEEALDKAIESIKDGVETNDIGKIIEDIITEGGFNPIKDLTGHNLEQ
                     YVLHAGVTIPNYKSFFSHTIKKDMVFAIEPFATYGRGKIKNGKPFIFAINDRCSGNIA
                     NEIRNRFGSLPFTPRWIPDTGINELKGAREYLELIENDDEIVAQSEHTVIVNEEGCEV
                     ITRLAK"
     gene            1915..2526
                     /locus_tag="VKL21_08015"
     CDS             1915..2526
                     /locus_tag="VKL21_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HMB45751.1"
                     /translation="MIKKDTIIYEAHGNLYLNITNRCTADCIFCLKRYSDGVYGYNLR
                     LLKEPTLPEIIKQLSEHELSKYKEVVFTGFGEPLVRLDDVIEITKWLTARGIQVRLDT
                     SGHAKLLYPDRNVAQEIAESGMKVVSLSLNAQNADTYNHLCDPKYIKAYDKMLEFAKD
                     ISNSGMTLRFTVVNLPIVNIEKCKQIAGEYSADFKVRGYSGSV"
     gene            2619..2888
                     /locus_tag="VKL21_08020"
     CDS             2619..2888
                     /locus_tag="VKL21_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMB45752.1"
                     /translation="MAGTRGGQTRALLLKLLIDRPYNANQLAEAMNMDYKTIRHHLDV
                     LIKNSVITMEGDKYGAMYFISKEMEANVNEFNQIWEKIDKQSNRT"
     gene            2951..3220
                     /locus_tag="VKL21_08025"
     CDS             2951..3220
                     /locus_tag="VKL21_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45753.1"
                     /translation="MLLVLLVSYFQIYNKIKSNFTLGLMIFTVALLLQSFSRAIMLLL
                     IINNPPPQHVYPIIINLLGYNPIYVIIPDVLEFVALSILLYFTRE"
     gene            3238..4059
                     /locus_tag="VKL21_08030"
     CDS             3238..4059
                     /locus_tag="VKL21_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HMB45754.1"
                     /translation="MRISCSSLFLWEYPVCDIMDILRKAGINSVEFWAETPFFWIGRN
                     DETTVAELKESISIMPNGCTMHAPIMDLNPSSYNDLVHEATIKETLWSLELARIIRAR
                     VVTIHPGKRTAHRKPTNEDWEKFMKYLEISAKKARSLGIGLSLENSMPGVQSMCSDPG
                     EMKEVLDQFPGLFFTFDVVHAFMGSPEMTLSFINELGDKIRNVHIGALHNGKPHCPVR
                     YEKKMDIVLRRLRDSGYNGDLTIEIDDKIYPGPLSRVEKIKELTDERKYIESIFD"
     gene            complement(4096..>5001)
                     /locus_tag="VKL21_08035"
     CDS             complement(4096..>5001)
                     /locus_tag="VKL21_08035"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMB45755.1"
                     /translation="IIISKNTVILICLILFTAGPATASTLNVGKDQAYTTIQSAIDAA
                     KTGDSILVDEGIYIENPVINTNGISIIGNNRENTIIDGKNKSSGIRIDQMNKVVISGF
                     TIKNSGGGGSEVAGVTIFNGNDNTVSNLNIIGNYVGISIFKESNNNTVSGNIVESNLG
                     QLGYGIYVHISHDNKIFNNNIKNNKVGIYLYDSKTNRIYSNNFLSNKDGQAYDQTGLN
                     VWDFDKMGNYWSDYNGSGEYVIKGAKNVLDNFPQKNAFSIKSEAIPAQSAQKANETGK
                     STPGFTIISFVISLIAIGMFIRRKK"
BASE COUNT         1564 a          941 c         1002 g         1494 t
ORIGIN      
        1 tatcgctttc aggaaggaat tgcacctatg tcatccagca gggtattcct gaaaatgctc
       61 ccgaaggtga aatcaggaaa gataaggcat ttacaaggga tgaacttgag gctttggccc
      121 tgagcatatc atttctgaaa gatgtgagga taagaacaaa agaaagaggg gaagaaaaaa
      181 tatgtggaat agagagtcct ttaaaggata tggcgaaata atcactgctg ccgtattgtg
      241 gagcctggca ggaatattcg ccaaacagat acatgggatg tctgcccaga gcattatttt
      301 ttacagggtc gtatttgctt ttgctatatt tttcatattt attttaattt cgaagaactt
      361 ccggataatt cagctaaaag ataagaaaat ttatttactg ttattcggca tactgcaggt
      421 aggtacaatg ctgacatttt ttatttcatt attaaaggga tcagtttcaa ttgcagtatt
      481 attattgtac acagctcctg tttatattac aatattatcc ccctggctct taaaagaaag
      541 atccacaaaa aaaggaattt tcgcccttat tttatcgata cttggcattc tttttatagt
      601 tgatcccgca aaacttgatc tggcgcatta cccggcaggg atacttgcag ggatagcatc
      661 aggtattatt tatgccttcc agataatgac ctcaaaatat gccagttcaa cttactcagg
      721 ttactcccag gctttctgga gttttccggt agcgatcatg atccttctcc ctgtaggaac
      781 cgcccctttt aatgtggtga tggataatct ggtttattta atacttctat ccatattccc
      841 cacgatcctg gcggtctcac tgtattttaa cgggttaaaa aaagtaaaca cacatagcgc
      901 aagtatagtt ggattaattg aacctttaag cgctgttatt ttatctgtac ttttccttca
      961 tgagcagata tcaattcttg aaataatcgg aggtgccctt atattggcag gagtttcatt
     1021 agtaaccata gatgaaccac tatattcatg attactacct tgaagccgga agaatagcgg
     1081 caaaagtccg aaccgaaacg gtttccatga tcaaagaaga tatccctcta cttgagatag
     1141 cggaatttgt tgaagaaagg ataaaagacc tgggcgccaa tcctgctttt ccctgtaata
     1201 tctcaataaa tgaaattgca tcccattaca ctccgcagga ttacctgccg tgtttcagga
     1261 aaggagatgt tgtaaaatta gacctgggga cccacatcga aggctatatt gcagacacag
     1321 cggcaactct tgaaatcggg acgaatcatc accacaaatt gatccgaaca tgtgaagaag
     1381 cgctggataa ggctatcgaa tccataaagg acggggttga aacgaatgat atcgggaaga
     1441 taattgaaga cataataaca gaaggaggtt ttaatcccat aaaagatctg acaggccaca
     1501 acctcgaaca atatgtactg catgcaggcg ttacaattcc taattataaa agttttttta
     1561 gtcataccat aaaaaaggac atggtctttg ctatcgaacc tttcgcaaca tacggaagag
     1621 gaaagataaa aaatggcaaa ccatttattt ttgcaataaa tgacagatgt tccggaaata
     1681 tagctaacga gatcaggaat agattcggtt cacttccttt tactccgaga tggatcccgg
     1741 atacaggaat taacgagcta aaaggagcaa gagaatatct tgaattgatc gaaaacgatg
     1801 acgaaatagt cgcccagtca gaacatactg ttattgtaaa cgaagaaggg tgcgaagtga
     1861 taacaagatt agcaaagtaa tatgaggtag gaaccacaat tataatgagg caagatgatc
     1921 aaaaaagata ccatcattta cgaggcgcat ggcaacttat atttgaatat tacaaataga
     1981 tgtactgctg attgtatttt ttgcctgaag cgttactctg atggcgtgta cggctataac
     2041 ttgcgtctct tgaaggaacc cacgcttcct gaaataataa aacagctttc agagcacgaa
     2101 ctctcaaaat ataaagaagt tgttttcaca ggcttcggag aacctctcgt gcgtctggat
     2161 gatgtgattg agatcacaaa atggcttact gcccgtggaa tccaggtaag acttgatacg
     2221 agcgggcatg caaaactcct gtatcctgac aggaatgtcg cccaggaaat agctgaatcg
     2281 ggaatgaaag tagtatccct cagcctgaat gcccagaatg cggacactta taatcattta
     2341 tgcgatccca agtacataaa ggcctatgat aaaatgcttg agtttgcaaa agacatttca
     2401 aattccggga tgacactcag gttcacagtg gttaatctgc ccattgttaa tatcgaaaaa
     2461 tgtaagcaaa tagccggtga atatagcgcc gacttcaaag tacggggtta tagcggttct
     2521 gtttgaacgc ttgttaccac aagttataat tattacatta ttcatttgtt cgttttgaca
     2581 ggagagagga ttttctgaag aagctgctct ggtatcttat cgcaggaaca cgtggcgggc
     2641 aaacccgtgc attactactg aagctgctca ttgacaggcc ttataatgca aatcaactag
     2701 ctgaggctat gaatatggat tataaaacca taaggcatca tcttgatgtc cttatcaaga
     2761 acagtgtaat aacgatggaa ggtgacaaat atggcgctat gtatttcatt tcaaaagaaa
     2821 tggaggcaaa tgtaaatgaa tttaatcaga tttgggaaaa aattgataaa caaagcaatc
     2881 gaacataaca ggaggaagtt ttattcagat aactgaaatc tcctattatg tctcggcagg
     2941 aaatatcgta ttgctcttag tactcctggt ttcatatttt cagatttaca ataagatcaa
     3001 atccaacttt acccttggtc ttatgatctt tacggtggct ctattattgc agagcttttc
     3061 cagggcgatc atgctgctct tgataattaa taacccgcct ccacagcatg tgtaccccat
     3121 aattatcaac ctgttgggtt ataatccgat atatgtgata atcccggatg ttcttgagtt
     3181 tgtggctttg agtatattac tgtatttcac cagggaatag aagatcatag gtggcagatg
     3241 cgaatatcat gctcgtctct ctttctctgg gaatatcctg tctgcgatat catggatatc
     3301 ctgcgaaaag caggtattaa tagcgttgaa ttctgggcag agaccccttt tttctggatt
     3361 ggccgaaatg atgaaactac tgtagccgaa ttgaaagaat caatttccat aatgccaaat
     3421 ggatgcacaa tgcacgcacc cattatggat ctaaatccat cttcttataa tgacctcgtg
     3481 catgaggcaa caattaagga aaccttatgg tctcttgagc ttgccagaat tattagagcc
     3541 cgtgttgtta ctatccatcc gggcaaaagg actgctcatc gaaaacctac gaacgaggat
     3601 tgggagaaat tcatgaaata tctggagatc agcgccaaaa aagcaagaag tcttgggatt
     3661 ggcctttcac ttgaaaacag catgcctggc gttcaatcaa tgtgttccga tcctggtgaa
     3721 atgaaagaag tgctggatca gttccccggt ctgtttttca catttgatgt agtacatgca
     3781 tttatgggat cccctgagat gaccttatct ttcataaatg aacttggcga taaaatcagg
     3841 aacgtccata tcggggccct tcataacgga aagccgcatt gccctgtgcg ctatgaaaaa
     3901 aaaatggata ttgtattgcg acgcttgcgt gattccggat ataatgggga ccttactatt
     3961 gagatagatg ataagatata tccaggacct ttatcgagag tggaaaaaat taaggaattg
     4021 accgatgaaa ggaaatacat tgaatcgata tttgactaaa aataatatta ggtgaagatt
     4081 ataactcaaa tgaaattatt tctttctcct gataaacatc cctattgcaa ttaatgaaat
     4141 aacgaatgaa atgatggtaa atcccggtgt ggattttccg gtctcattag ctttttgggc
     4201 tgactgagca gggatagctt cagactttat agaaaaagca tttttctggg ggaaattatc
     4261 caaaacattt ttcgcacctt taataacata ttcgccgctg ccattataat cactccaata
     4321 atttcccatt ttatcgaaat cccatacatt caggccggtt tgatcataag cctggccatc
     4381 tttgttgctt aggaagttat ttgagtatat tcgatttgtt tttgaatcgt ataaataaat
     4441 cccgacctta ttgttcttta tattgttatt gaaaatttta ttgtcatgtg aaatatgaac
     4501 ataaatacca tatcctaact gtcctaagtt tgattccaca atatttcctg aaacagtatt
     4561 gttgttgctt tccttaaata tcgaaatacc tacgtaatta ccaatgatat ttaagttgga
     4621 aactgtattg tcatttccgt taaaaattgt aactcctgcg acttctgaac ccccgccgcc
     4681 gctgttcttt atggtaaatc cgctgattac cactttattc atttgatcaa tcctgatccc
     4741 gctgcttttg ttcttaccat cgatgatggt gttttccctg ttattaccga taattgaaat
     4801 cccgttcgta ttaataacag gattttcaat ataaatgcct tcatcgacaa gaatagaatc
     4861 cccggttttt gcagcgtcaa tagccgattg aatagttgta taagcctggt ccttgcccac
     4921 attaagagtt gatgcagttg ctggccctgc tgtaaataat atcagacaaa tcaatataac
     4981 agtatttttt gatataataa t
//
LOCUS       DATGBN010000329         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_142720, whole genome shotgun sequence.
ACCESSION   DATGBN010000329 DATGBN010000000
VERSION     DATGBN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142720"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VKL21_08040"
     CDS             <1..975
                     /locus_tag="VKL21_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB45756.1"
                     /translation="SCNNNYSHDLKTQPDEIIGKTDYDFYNKELAEKYRADDKRIMES
                     GKTEYIEEKYIQNGQEVVVQTVKTPLEDKIGNKIGILGIFWDITERRRIEEALKESEE
                     RYRTIVDYSNDMIWTMDTEGRFLFFNKRSEEITGFKLEDWQGKSFAPLIEKDDLPKVI
                     EVFHKTLGGEPVQYEVSVAKEDGIKIILLVNTAPIYSKEKVVGTVSFGRDITERKKAE
                     EQIKESLKQKLKISNKQNKLLCYLIEGTRGGRTRALILRHLAEKSYNANQLAKTLGIE
                     YKTIRHHLRVLVKHGMITRNHDGYPDLYFIPNNIAESLYSFDDKKEKL"
     gene            complement(1145..2143)
                     /locus_tag="VKL21_08045"
     CDS             complement(1145..2143)
                     /locus_tag="VKL21_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45757.1"
                     /translation="MAKCNNILIRDIPLLGCIAFGIIDRGTNVLQVRPISECPLSCIF
                     CSTDAGPYSQKRISEYMVDLAQLIGGFEWAASYKEIDDIEAHIDTVGEPSMYPQLVEL
                     VGRLSENKSVKTVSMQTNGFLLNEKLIDELDSAGLSRINMSIEALEPDLARKIAGIDS
                     YNIDRVLRNAQYIARNTGIDLLIAPVWLPGINDAEIPKLIEFALKLGAGKKWPALGIQ
                     KFLQHKNGRKPEVKVMSWEKFYSQLEEWERKYQTKLILSPEDFGSHDSKALPRMFRRY
                     EKVKVEIIGPGWMKGEKLAIARDRVITIVDAGNIPVGKEISVRIERVVDGIYMAKR"
     gene            complement(2253..>2410)
                     /locus_tag="VKL21_08050"
     CDS             complement(2253..>2410)
                     /locus_tag="VKL21_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF166 family protein"
                     /protein_id="HMB45758.1"
                     /translation="KLDEVISNAHHAYPCTASMERDAETKDTILHKAGYIIREAVHGA
                     LNENNVA"
BASE COUNT          738 a          500 c          490 g          682 t
ORIGIN      
        1 tcctgcaaca ataactattc tcacgatttg aaaactcagc cagatgaaat tattgggaag
       61 acagattatg atttctataa caaggagctt gctgaaaaat acagggcaga tgataaaaga
      121 attatggagt cgggcaagac ggaatatata gaagagaaat atattcaaaa tggacaagaa
      181 gtcgtcgtcc agactgttaa aacacctctt gaagataaga taggcaataa gattggcata
      241 ctgggcattt tctgggatat tactgaacga aggcggattg aggaggctct gaaagaaagc
      301 gaagagcgtt accgcacgat agttgattac tccaatgaca tgatctggac tatggacacc
      361 gaaggacgtt tcttattctt caataagcgc tctgaagaga taacaggatt taaattggag
      421 gattggcagg gcaagtcctt tgctccgctc atagaaaaag atgatttgcc taaggtaatt
      481 gaggtattcc acaaaacact gggtggagag cctgtgcagt atgaggtatc agtcgcaaaa
      541 gaagatggta ttaaaattat tcttttagta aatacagctc ccatatattc aaaagaaaaa
      601 gttgtgggaa cggtgagttt tggccgggac ataacagagc gtaagaaagc agaagagcaa
      661 ataaaagaat ctcttaaaca aaaattaaag atatccaata aacaaaacaa gctcctatgc
      721 tatctgattg aaggaacacg tggaggaagg acccgcgcac taatcttgag acatcttgcc
      781 gagaaatctt ataatgcgaa tcaattagca aaaactctag gaattgaata taaaaccatc
      841 aggcatcatc ttagggtttt agtcaaacat ggaatgatta caagaaacca tgatggatac
      901 cctgatttat atttcattcc aaacaatatt gcagaaagcc tgtattcttt tgatgataaa
      961 aaagaaaaac tctgattctc tattaggtgc ttgggtttgg gtaaagttcg ggaaaagtca
     1021 tttaaatttc agaatcattg aagcctttgc agaaaaaaat atggtatgga aggggggtgg
     1081 tatccctttc tgcaaaggcc aatctattct ttagattgaa gatataaaga ctttttgacg
     1141 tagttcatct ctttgccata tatatcccat ccactaccct ttcaatcctg accgatattt
     1201 cttttcccac agggatattg ccagcatcaa cgatcgttat taccctgtcc ctggcaatgg
     1261 caagtttctc acccttcatc cagccgggcc cgataatttc tacttttact ttttcatatc
     1321 tccgaaacat cctggggagc gccttactgt catgacttcc aaagtcctca ggagaaagga
     1381 tcagctttgt ctggtatttc ctttcccatt cctcaagctg tgagtagaat ttctcccagc
     1441 tcatcacttt aacttccggc ttccttccgt tcttatgttg aaggaatttc tgtattccaa
     1501 gagcaggcca ttttttcccg gctcccagct tcagggcaaa ttcaatcaat tttgggatct
     1561 cagcatcatt aattcccgga agccacacag gtgcgataag aaggtcaata ccagtatttc
     1621 tggcaatata ttgtgcattc cgaaggaccc tgtcaatatt ataagaatca atacctgcta
     1681 ttttccttgc gaggtcgggc tcaagcgctt ctatggacat attgatccgg gaaagcccgg
     1741 cactatccag ttcatcaatg agtttttcat tcaataagaa tccattggtc tgcatggaaa
     1801 ccgtcttcac acttttattt tcagaaagcc tccctacaag ttcaaccagc tgcggataca
     1861 tggaaggctc tcccacagtg tctatatgcg cttcgatatc atcgatctcc ttatatgagg
     1921 cagcccattc aaagcctcct attagttgcg caaggtctac catatattcc gatatccttt
     1981 tctgtgaata aggaccagca tcggttgaac aaaaaataca ggataaagga cattcactta
     2041 tggggcgcac ctgcaataca tttgtcccgc ggtcgatgat gccaaatgcg atgcagccaa
     2101 gaagagggat atcgcggatg agaatgttat tacattttgc catttatcct cgtggggtag
     2161 aaaagccccc ctccgaaagg tgtggataaa ccccttgcga ttgtaatatc tatcccccta
     2221 aaaagagttg tcgattctgc atgaaaaaaa aatcatgcta cattattttc attgagagcc
     2281 ccatgaaccg cttccctgat aatatatcct gctttatgca gtatcgtatc ttttgtttcg
     2341 gcatctcttt ccatgctcgc agtgcaggga tatgcgtgat gcgcatttga gatgacctca
     2401 tcaagtttac
//
LOCUS       DATGBN010000330         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_297846, whole genome shotgun sequence.
ACCESSION   DATGBN010000330 DATGBN010000000
VERSION     DATGBN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297846"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VKL21_08055"
     CDS             complement(<1..448)
                     /locus_tag="VKL21_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMB45759.1"
                     /translation="MKLNSPLQVYKYLPQTNCAECGEATCMAFASHLIVRSKKIDDCP
                     PILKEGFKKKYLELQTLLAPEIREITIGTGEHAKRIGGDDILYRHQLTFFDPTALAYD
                     VWDTMPEKDLVERVNKIQNFRKFYVGKFLKVDMVAVRCVSGDALKFA"
     gene            complement(448..1737)
                     /gene="cdhD"
                     /locus_tag="VKL21_08060"
     CDS             complement(448..1737)
                     /gene="cdhD"
                     /locus_tag="VKL21_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase subunit
                     delta"
                     /protein_id="HMB45760.1"
                     /translation="MTKKIKLSELGTLLGGDIEALEGVKIEGDVEIDLGQTGMNPQMA
                     YALGHETAQMAMHLMNISRILGYPVDQMLGGPQVSAPKRLSTLIESKFSVGKIEEWKT
                     PIQEVVLGATSSDGGSRKSTITLGGENALPYYFDSPMPHRNHITMDVFDMPINMAKAV
                     KSNYEDVINSPAEWAKKVVRDFGADMVTIHLISTDPNIKDTSPAQAAKTVEEVLQAVD
                     VPIVIGGSGNPEKDPAVLEKAAEVAEGERCILASASLNLDYARIAEAAKKYNHVVLSW
                     TQLEINAQKELNRKLMKQCGVARENILMDPTTAALGYGLDYAYTNMERIRLAGLSGDT
                     ELNFPMSSGTTNAWGAREAWMTSSPLKEDSDWGPREYRGPIWEIITGLTLSLAGNDLF
                     MMMHPTAVQVLKEITQTLYGLKETEPSNIDNWIATVI"
     gene            complement(1954..2109)
                     /locus_tag="VKL21_08065"
     CDS             complement(1954..2109)
                     /locus_tag="VKL21_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45761.1"
                     /translation="MERPRIKLHGNSTILLTISDIFFFGALENDACMFGEVLSFTAKT
                     SLIEVFA"
     gene            complement(2166..2954)
                     /gene="purQ"
                     /locus_tag="VKL21_08070"
     CDS             complement(2166..2954)
                     /gene="purQ"
                     /locus_tag="VKL21_08070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HMB45762.1"
                     /translation="MTPKVLVLTGYGINCDIETQHAFKLAGARAERVHINDLINGKER
                     LSDYHILSFPGGFSFGDDIASGKVLANMVKYNIGEQIQEFIDSGKLILGICNGFQVMV
                     KMGLLPGFNDDYATQEVTLTYNDSGRFEDRWVHLKANKISKCVFTKGMENIYLPVRHG
                     EGKFVAKNPQVLEKLKRGNHIVFQYVDTEGKSAGYPHNPNGSVNNIAAICDKTGRVFG
                     MMPHPEAFQHRTNHPGWTREELPEEGAGIAIFRNAVEYVKKNSY"
     gene            complement(3109..>4115)
                     /locus_tag="VKL21_08075"
     CDS             complement(3109..>4115)
                     /locus_tag="VKL21_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMB45763.1"
                     /translation="AAWSEICIVYSEVITMVDQQSLFWIGFIVLIFILLALDLGVFNR
                     KAHVIKMKEAILLTLFWISISVMFNIWIYFQFGEQKAFEFLTGYVIEKALSVDNLFVF
                     LMVFSYFHVPSIYQHKILFWGILGALIMRAIFIAVGVTLIEQFHAVIYIFGAFLVITG
                     IKMALMGDKKIDPEKNPVLKYLRKIVPVAENYHEDKFFIKRAGKYFVTPLFVVLVVIE
                     STDIIFAVDSIPAILSITTDPFIVYTSNVFAILGLRALYFALAGVMPMFYYLNYGLSA
                     ILAFVGTKMLISDYYKVPIFVSLGVVAAILIASVALSVIRARLLEYRARELKKLQEQE
                     "
BASE COUNT         1106 a          973 c          832 g         1204 t
ORIGIN      
        1 ctgcaaattt aagggcatca cccgaaacgc atcttacggc taccatgtca actttcagga
       61 acttgcctac atagaattta cggaaattct gaatcttgtt tactctttct acaaggtctt
      121 tttcaggcat tgtgtcccat acgtcatatg ccaatgcggt ggggtcaaag aaagtcaact
      181 gatgacggta aaggatatca tcccccccga tcctttttgc atgttcaccc gtgcctattg
      241 taatttcacg tatctccggg gcaagaagcg tctgtaattc cagatatttt ttcttgaaac
      301 cttccttgag aatgggcggg caatcatcaa tcttctttga cctgacgatg agatgtgacg
      361 caaaggccat gcatgttgct tcaccacatt ctgcgcaatt tgtctgtggc aggtatttat
      421 atacctggag agggctgttc agtttcatta tatcaccgtc gcgatccaat tatcaatatt
      481 cgatggctct gtttctttca aaccgtacaa tgtctgggta atttccttga gaacctgtac
      541 tgcagtggga tgcatcatca tgaaaaggtc gttcccggca agagataaag tcaatccggt
      601 gataatttcc catatcggtc cccgatactc acgaggtccc cagtcagaat cttccttcaa
      661 gggtgatgag gtcatccatg cctcacgcgc cccccatgca ttagtggtgc cgcttgacat
      721 cgggaaattg agttcggtat cccctgagag accggcaagc cttatccttt ccatattcgt
      781 ataagcataa tcaaggccat atccaagagc ggcggtcgtg gggtccatca atatattttc
      841 gcgcgccaca ccgcattgtt tcatgagttt gcgattcaat tctttttgcg catttatctc
      901 aagttgggtc catgaaagca ccacatggtt atatttcttg gccgcctcag cgatcctggc
      961 ataatcaagg ttcaaactgg ccgatgcaag aatacacctt tcgccttcgg cgacttctgc
     1021 ggctttctca agaaccgccg ggtctttttc ggggttgccg gaacctccga tcacgattgg
     1081 aacatccact gcctgtagaa cctcttccac ggtttttgcc gcctgcgccg gtgatgtgtc
     1141 cttgatgtta gggtctgtgc tgataagatg aattgtgacc atatccgccc cgaaatcccg
     1201 gacaactttc tttgcccatt ctgcaggaga attgatgacg tcttcataat tggattttac
     1261 cgcttttgcc atgttgatag gcatatcaaa aacgtccata gtaatatgat tgcgatgcgg
     1321 catcgggcta tcaaaataat agggaagggc attttctccg ccaagcgtta ttgtactctt
     1381 tctgcttcca ccgtccgatg aagtcgcccc aaggaccact tcctggatgg gagttttcca
     1441 ttcctctatt tttcccacgc tgaatttcga ctcgattaaa gtgcttaatc tctttggagc
     1501 agaaacctgt ggtcctccta acatttggtc aaccggataa cctaaaattc ttgaaatatt
     1561 catcaagtgc atagccatct gggcagtctc atgacctaac gcataggcca tctggggatt
     1621 catacccgtc tgtccgaggt ctatctcaac atcaccttct atttttactc cttcaagggc
     1681 ttcaatatcg ccgccaagca gggttccaag ttcagagagt ttgatttttt ttgtcattct
     1741 tacatcccat ttcgcactat taatcgtcca agatagggct ggttacatat atatttttat
     1801 atttcggaat gcgcgattta tataaaaatt tacatgctca tcaaatttaa ataataaaac
     1861 atctattgca agctgaataa aaatttttga aaaaattgct caggagaaaa acagatggat
     1921 ctcacaattg gaaaatgcgg tattgcctgt gaattatgca aaaacttcaa taagggatgt
     1981 tttggctgtg aaactgagaa cttctccaaa catgcatgca tcattttcaa gtgcgccgaa
     2041 aaaaaaaatg tcagatattg tcaacagtat tgtggagttt ccatgcaatt ttatgcgagg
     2101 tctttccaaa tcatattgtc ccgtattcac ttcgataaaa atatcctgag tgaaaaccac
     2161 atttattaat acgaattctt cttcacatac tcaaccgcat tccgaaagat cgctatgcct
     2221 gcaccttctt ccggcaattc ttcgcgtgtc catccgggat ggttcgtcct gtgctggaat
     2281 gcctcaggat ggggcatcat cccgaaaaca cggcctgtct tatcgcatat tgccgcgata
     2341 ttattcaccg agccgttggg attgtgggga tatcccgcag attttccttc tgtatccaca
     2401 tattgaaaaa caatatgatt tcctcttttt aacttttcaa gaacctgcgg gttctttgcc
     2461 acgaacttac cctcaccgtg gcgtactgga aggtaaatat tttccatccc tttcgtaaag
     2521 acacattttg atattttatt tgccttaagg tgtacccacc tgtcctcgaa tcttcccgaa
     2581 tcattatagg taagtgttac ttcctgggtg gcataatcgt cattaaatcc tggcagcaag
     2641 cccattttca ccataacctg gaaaccattg catataccaa ggatcaattt tcccgaatct
     2701 atgaactcct gtatttgctc tccaatatta tatttgacca tatttgcaag tactttgccg
     2761 gatgcgatat catccccgaa tgaaaagcct ccagggaatg aaaggatatg ataatcagaa
     2821 agtctttctt ttccattgat caggtcattg atatgcacac gttcggccct ggctccggca
     2881 agtttgaatg catgctgggt ttcaatatcg cagttgatgc cgtagcctgt gaggacaaga
     2941 acttttggag tcattgattt tcctataatc tttaggtttt acctatatct tgaattaatc
     3001 cataataagg tcaccggggg acacgtatta ccttataaaa cgtaccatgt tcagatgtgt
     3061 gctcataagt tccatgattt tatacgcttt ctaaccttca cttttcaact actcttgctc
     3121 ctgaagcttc ttcaactccc tcgccctgta ttccagcaga cgggcgcgaa taactgataa
     3181 cgccactgat gcgatgagaa ttgcagctac gacgcccagg gagacgaata tagggacctt
     3241 ataataatcc gagatcagca tctttgtgcc cacaaaggca agtatggctg aaagcccgta
     3301 attgagatag tagaacatag gcatgacccc tgcaagagcg aaatagagcg cccgcagccc
     3361 caggatggcg aacacgttgg aagtataaac gataaaaggg tctgttgtga tagaaaggat
     3421 agcgggtatg gaatccactg caaagatgat atcagtgctc tcgataacga ccagtacgac
     3481 gaacagagga gttacgaaat atttccctgc tctctttatg aagaacttat cctcgtggta
     3541 attttctgca acaggcacaa tctttcgtaa gtactttagc accgggtttt tctctgggtc
     3601 tatcttttta tcacccataa gcgccatctt gatgcctgta attaccagga acgccccgaa
     3661 gatgtaaatg accgcatgga actgctcgat gagcgtcacg cccacagcta tgaatatcgc
     3721 acgcataatg agagcgccca ggatgcccca gaaaaggatc ttatgctggt agatcgatgg
     3781 tacatggaag taggaaaaaa ccattaaaaa cacgaacagg ttgtcaacgc tgagtgcctt
     3841 ctctatcaca taccctgtca ggaactcaaa ggctttctgc tcaccgaact ggaaatatat
     3901 ccagatgttg aacatgactg atatcgatat ccagaaaagg gtcagtaaaa tggcttcctt
     3961 catcttgatg acatgggctt tcctgttgaa aactccaaga tccagagcca gcaatataaa
     4021 tatcagtaca ataaatccta tccaaaacag gctttgttga tctaccattg ttattacctc
     4081 ggaataaaca atgcaaattt cgctccatgc cgccc
//
LOCUS       DATGBN010000331         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_104042, whole genome shotgun sequence.
ACCESSION   DATGBN010000331 DATGBN010000000
VERSION     DATGBN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104042"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VKL21_08080"
     CDS             complement(<1..1163)
                     /locus_tag="VKL21_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HMB45764.1"
                     /translation="MKNKGVPASKEAINELTGLLIKLYHIKTMKDTDQVMVYIDGYYH
                     LDGEKDIRSEVNQVFQDNYTSKIFGDILDKIQASTTISRAEFQEPLNKINLKNGVLDI
                     RSKGLDLHNPDFNFLYKLNIDYNPAQDCPAIKQFLHEVTANENDYQCLQEYIGYTLLS
                     GYPFRVFLVLYGSGANGKSIFIYLIRNFIGEDNVSVVSLQQLQADRFAPARLYGKKAN
                     VFADIPPLKMKSTDIIKSLTGFTDKISGEKKFKDTFEFVNGAKLIFSANQLPQVDDET
                     DAFWNRVILIDFPNKFEGKKDDKELIKKLTTREELSGLLNFALQGLDQLLKNGRFTYD
                     HDTTYERWQKNTEVENPVQDFMEKCIETRIDALATKEALYDAYVVYCHMVTKPP"
     gene            complement(1227..1484)
                     /locus_tag="VKL21_08085"
     CDS             complement(1227..1484)
                     /locus_tag="VKL21_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45765.1"
                     /translation="MYKTMIGILHGETQKIIKICEDIAAKDNSFAYYIIRPTVKEIKY
                     KFENVLVLLSDSEKEAKSRGGWFIHRCENAKIPDYFWIKKA"
     gene            complement(1487..2368)
                     /locus_tag="VKL21_08090"
     CDS             complement(1487..2368)
                     /locus_tag="VKL21_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45766.1"
                     /translation="MGEKSIKEIMDERFGVHQIPKRQVSREEMNNLIAEAKERGRTKS
                     ERKKITENEERTDKSGNPCPKCASTNWNFLPDNRKYCWKCGFVEEPEKITCEFKCDIC
                     GKVIQFPGKNIEVAREKAVLHKDYDSNTECEGIFKFERQVEAISSISEPIKETEKNMG
                     KILFPFHCNLCGIVVQFQATNPEIAMEKAIPHKKYDPKTNCDGTFTLLKHINPDEIRK
                     RPVIPRESIGISDKFYHPKSDKFICGGCYDLNQCLQTSHHMEICLDKNIDLKLKTMLE
                     ILSEILKILSKNKMAGE"
     gene            complement(2370..2738)
                     /locus_tag="VKL21_08095"
     CDS             complement(2370..2738)
                     /locus_tag="VKL21_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45767.1"
                     /translation="MDKIELENFSGIYLKLDFDDVPNRIYEAQVCLHNIMKPFYVRVS
                     SGKRGFHVLKFCTNCDWLVSVWDDKRRRIINEVRKRNGFIGNILFDIKTVRGKRRAAG
                     EWQLIKDSYDVERFIDFWRP"
     gene            complement(2738..3451)
                     /locus_tag="VKL21_08100"
     CDS             complement(2738..3451)
                     /locus_tag="VKL21_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45768.1"
                     /translation="MVLLSGSAFERQEEGIRMFMGLSQNRGIKEDSVNIKKILQFAEL
                     IKFDVESVMRILAGEQALSPEQIKKIRYLHAEEVGKVRVPAPRSILDLVIIPPEPLGI
                     EGRKCSLMIHIDALEHNLIAYFAINYKDFLTEYVEYPSYDGAIEHPRNFMNEFFVNFT
                     RVKDKSMGLVESELSLEVRYKEECPKKYRVFEMELIEIETETLRYGLKYANGRVLKSV
                     GNLPVKEIVKILESVGVWQ"
     gene            complement(3589..3780)
                     /locus_tag="VKL21_08105"
     CDS             complement(3589..3780)
                     /locus_tag="VKL21_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45769.1"
                     /translation="MLYEKYETRTQFYRRPWNRLTAEEKEEIIIKSNLKKIEEMLKEN
                     KKKVEIMTGKQRTIDDYFL"
BASE COUNT         1025 a          837 c          614 g         1526 t
ORIGIN      
        1 ggtggctttg tgaccatatg acaataaaca acatatgcat catatagggc ctctttagta
       61 gccagcgcat ctatccttgt ttcaatgcac ttttccatga aatcttgaac aggattctca
      121 acttcggtat tcttttgcca tctctcataa gtggtatcat ggtcgtatgt gaacctgcca
      181 tttttaagca gttggtctaa accttgcagg gcgaaattta gtagtcctga taattcctct
      241 cgtgttgtta atttttttat tagctcctta tcatcctttt tcccctcaaa cttattagga
      301 aaatcaataa ggattactct gttccaaaat gcatctgttt catcatctac ctgcggcaat
      361 tgattggcgg aaaatatcaa ttttgctcca tttacaaatt caaatgtgtc tttgaacttt
      421 ttttctccag atatcttatc agtgaatccg gttagtgact ttattatgtc tgtagacttc
      481 attttcaatg gtggtatgtc ggcaaacaca tttgctttct tgccgtacaa tcttgctggt
      541 gcaaatctgt ctgcctgtag ttgctgcaaa gatactacag atacattatc ctctccgata
      601 aaatttctga ttaagtatat aaatatggat ttcccatttg ctccactacc atatagtacc
      661 aagaatactc ggaaaggata tcctgataac agtgtataac caatatattc ttgcaggcat
      721 tgatagtcat tttcattggc tgttacttca tgcaagaatt gctttattgc agggcaatcc
      781 tgggcaggat tgtaatcgat atttaattta tataaaaaat taaaatctgg gttatgcaga
      841 tctaatcctt ttgacctaat gtccaggact ccatttttca ggtttatttt atttaggggt
      901 tcctgaaatt ctgccctact gattgttgtt gatgcttgta ttttatccag gatatcccca
      961 aatatttttg atgtataatt atcttgaaat acctggttaa cttcacttcg aatatccttt
     1021 tcaccatcta aatgataata cccgtcaata tacaccatta cttggtcggt gtctttcatg
     1081 gtttttatat gatataattt tattaaaaga ccagtgagtt cattaattgc ttctttacta
     1141 gctggtacgc ctttgttttt catttatatc gcctctgcta ttggttttat tcaaaatcat
     1201 caaaaattca gattgttgtt accgaactaa gcttttttaa tccagaaata atctggaatc
     1261 tttgcatttt cacagcggtg gatgaaccag ccgcctcttg atttggcttc tttctccgaa
     1321 tcggataata atactaaaac attttcgaat ttatatttaa tttcttttac tgtgggtctt
     1381 atgatatagt aggcaaaaga attatccttt gccgctatat cttcgcatat cttgattatt
     1441 ttctgagtct caccatgcaa aatacctatc attgttttat acatgattat tctcccgcca
     1501 ttttattttt ggatagtatt ttcaatattt ctgagagtat ctcaagcatg gtttttagct
     1561 tgaggtcaat gtttttatca agacatattt ccatatgatg gcttgtttgc agacattgat
     1621 tcaaatcata gcacccgccg caaatgaatt tatcactttt tggatgataa aatttatctg
     1681 agattccaat gctttccctg ggaattacag gtcgctttct aatttcatcg ggattaatgt
     1741 gcttcaataa agtgaatgtc ccatcacaat ttgttttggg atcatacttc ttgtgcggga
     1801 tggctttttc cattgcaatt tctggatttg tagcctggaa ttggactact atgccacaca
     1861 ggttgcaatg aaaaggaaat agtatctttc ccatattttt ttcggtttct ttgattggtt
     1921 ctgagatcga gctgatggct tcgacctgcc gttcaaattt gaatattcct tcacactcgg
     1981 tatttgaatc ataatcctta tgcagaactg ctttttcccg tgcaacttca atattcttcc
     2041 ctggaaattg gattactttg ccgcatatgt cgcatttgaa ctcacaggtt attttttctg
     2101 gctcttcgac aaagccgcat ttccagcagt attttctatt atcaggtaag aaattccagt
     2161 ttgttgaagc gcattttgga caaggatttc cagatttatc tgttcgttct tcattttctg
     2221 tgatcttttt cctttcactt ttagttctgc ctctctcttt tgcttctgca atcaggttat
     2281 tcatttcttc cctagatact tgtctttttg gaatttgatg aacgccaaag cgttcatcca
     2341 tgatttcttt tattgatttc tcacccattt caaggcctcc agaaatcaat aaatcgttct
     2401 acatcatagg aatctttgat aagctgccac tctccggcag cccgcctctt accccggaca
     2461 gtttttatgt caaaaagtat gtttcctata aaaccatttc ttttcctgac ctcattgata
     2521 attctccttc gtttatcatc ccaaaccgac acaagccagt cgcaattggt acagaattta
     2581 agaacatgga agccccgttt gccactgcta acccggacat aaaatggctt catgatgtta
     2641 tgcaggcaaa cttgggcttc gtatatacga ttgggaacgt catcgaagtc tagtttgagg
     2701 tatattcctg aaaagttctc tagttcaatt ttatccatta ctgccaaact cctacactct
     2761 ctaaaatttt tactatttct ttgaccggaa gatttccaac agattttaga actcgcccgt
     2821 tggcgtattt tagtccataa cggagggtct ctgtttcgat ttcaattaat tccatttcaa
     2881 acaccctata tttttttgga cattcttcct tatatctcac ctcgagtgat aattcactct
     2941 ccaccagtcc catgctttta tctttcaccc tcgtgaaatt gacaaagaat tcgttcataa
     3001 agttccgtgg atgttcgatc gcgccatcat agctcggata ttcaacatat tctgttaaaa
     3061 aatccttata attgatcgca aaatacgcga tcaagttatg ctcgagagcg tctatatgaa
     3121 tcattaatga gcattttcgt ccttcaattc caagaggctc tggtggtatt atcaccaggt
     3181 ctaatattga tcgaggtgct ggaaccctta ctttgcctac ttcttctgca tgcagatatc
     3241 taattttttt tatctgttca ggcgataaag cctgctcacc agctaatatc ctcattacgg
     3301 attcaacatc aaatttaatt aattctgcaa attgcagaat tttcttgata ttgacagaat
     3361 cttcttttat tcctctattc tgtgagagcc ccataaacat tctaatgccc tcttcttgcc
     3421 tttcaaaagc gcttccgctc aataatacca taaacatctt ctccttctat tcttttttaa
     3481 agggtggccg tgcagagcac ggtcttaacc ctaagtactt tccttaaaat ttaacccatt
     3541 cgtgttcagc aatttgatac gatctcctcc agctgatctt gtactttgct ataagaaata
     3601 gtcgtcaatc gttctctgtt tcccggtcat tatttcaact tttttcttat tttctttaag
     3661 catctcctct atctttttga ggttgctttt aataattatc tcctctttct cttcggctgt
     3721 aagtcgattc cacggtcttc tataaaattg tgttctagtt tcatattttt catataacat
     3781 ataaattctc caatttattt tctatttccc ttattttatt ctttagtatt ctaattcctc
     3841 aagacatatt ctgggattgt taattataca ggatggccta aaggccatcc actgttcttc
     3901 tttttttgat tcgcgtcgct cttctcgatt ttttccatcg tttaaaatgc tcctttatct
     3961 aatttatttt aaaattcttc ccaatatttt catattgcct cc
//
LOCUS       DATGBN010000332         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_64808, whole genome shotgun sequence.
ACCESSION   DATGBN010000332 DATGBN010000000
VERSION     DATGBN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64808"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..698)
                     /locus_tag="VKL21_08110"
     CDS             complement(117..698)
                     /locus_tag="VKL21_08110"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HMB45770.1"
                     /translation="MSPQDQKTSTSNEESDFRSTQEYYDQWLNIYKATMGRLVEMPAV
                     GPAREKSEKTMKGLSTFINLYVAGMDISSNLQTVFAEAMRRVQEKTANDKEGKISPGN
                     YKEFYKIWMETYSEAFKEFSKSDHFVSDLGKLNSFLIDFQQNNREIIEENYLKPMNLP
                     TKTEIEEINKELYSLKKTVKELVREIKELKENK"
     gene            822..1640
                     /locus_tag="VKL21_08115"
     CDS             822..1640
                     /locus_tag="VKL21_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HMB45771.1"
                     /translation="MYRIKTGDFNLDYSLSCGQVFRWEKNDWWTGIVNGATVRAKQEG
                     GEIIVDSSLDKDFIIGYFRLDDDIKQIYQSINRDEKIGSLINKYKGLRIIRQDPWECL
                     VSYMCSSNNTIRNITNSIRRMSECFGKEIEKGLYSFPTPFALSDVELCDMSQCRLGFR
                     SPRILKIANVVARGEFDLYGLKDLSYRKGREELVKIEGVGNKIADCVLLFSFGKLESF
                     PVDTHIEQIMERYYGFNFKGAKSKKRDEIAEFARSYFGMYCGYAQEYLYMEDLG"
     gene            1754..2221
                     /locus_tag="VKL21_08120"
     CDS             1754..2221
                     /locus_tag="VKL21_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMB45772.1"
                     /translation="MIHILENIDKWKCEYKKCGAKCCSEGIQLTIEDIKRIKELGYSP
                     DDFLDFDDKNNIFKIKGKEGKCFFNGKKLECTIRENEPLVCRLLPFRIIEVSYSDEPL
                     MKLKPVVDCPGVGQGKKIDKKKIEADATKFLRENQKLIRDVRKKGMKGIIAEL"
     gene            complement(2308..2886)
                     /locus_tag="VKL21_08125"
     CDS             complement(2308..2886)
                     /locus_tag="VKL21_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMB45773.1"
                     /translation="MKEFLDLDKRDREILSILEKYPEMSQSEIAEKLKISQPSVSARI
                     YKLKKKGALSHVVGMNLKKVGLYMAKVDVIANNTSSVLDIFKDCPYFLNGLIVSGKHN
                     LCLFFVGEDIATLEAIVDGHLRSNPLVRGAEVSIVIATMKDLVMPIKMNFEFSEMPPC
                     GNGCNCKDCAHHISNRCLGCPVTNSYNGKIWK"
     gene            3012..3725
                     /locus_tag="VKL21_08130"
     CDS             3012..3725
                     /locus_tag="VKL21_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45774.1"
                     /translation="MNRDIVFIDEEKCNGCGLCIPNCAEGAIKLINGKAKLVDDRFCD
                     GLGACLGHCPQDAITITKRDAKEFDEEAVKKHLKAEPTKVMKKAEKNIPSGCSGSMAM
                     DLRSEKKSDAPGRQSAQLRQWPVQLTLVSPEASYFKDSDLLVAADCVPFAYPNFHSDF
                     LKGKSLIIGCPKLDDTDFYIEKLTELIKKNNLKSITLVNMEVPCCFGLQRIVEEAIQK
                     AGKAIPIRQTVITIRGEKQ"
     gene            complement(4051..>4566)
                     /locus_tag="VKL21_08135"
     CDS             complement(4051..>4566)
                     /locus_tag="VKL21_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HMB45775.1"
                     /translation="RRLRFVEQYGITDDHAKALTSERKFAIFYEDVAKKADPKMAAVW
                     VCDVLKGELNYRDLAIEAFEKEHMLSVIDLLVNRKITEDGAVDIIRTLLDKGGSPDEI
                     VKEKGLLKVEGDIVSTAAEEAIKENPQTVADYRSGKEKALNSLVGAVMKKTKGRADAR
                     MAREMLLNRLK"
BASE COUNT         1329 a          926 c          948 g         1363 t
ORIGIN      
        1 cggaccatgg aaaatcacta tagaatcgcc tttgattccc atgctgtaag cccacaacgg
       61 cgcgatctgc gagccatcat attttattgg ttctgtaagg attattgagt tcatgattac
      121 ttgttctctt ttagctcttt gatctctctt accagttctt ttactgtctt cttgagagaa
      181 tataactctt tattgatctc ctcgatttct gtctttgtgg ggagattcat aggtttcaga
      241 taattttcct caattatttc cctgttattt tgttggaaat ctattaaaaa agaattcaat
      301 ttacctaaat cagacacgaa gtgatcggat ttcgagaatt ccttgaaggc ctcgctatac
      361 gtttccatcc agattttgta aaattcctta taattcccgg gactgatttt cccttcctta
      421 tcgtttgcag tcttttcctg caccctgcgc attgcttctg cgaatacggt ctgtaagtta
      481 gaactgatat ccatcccggc cacgtaaagg ttaataaagg tagagagacc cttcatcgtt
      541 ttttcagatt tctcccgtgc cggacccaca gcaggcattt cgacgagtct gcccattgtg
      601 gccttgtata tatttaacca ctggtcataa tattcctgtg tggatctaaa atcggattct
      661 tcattacttg tacttgtttt ttgatcttga gggctcattt cttattcttt ccacaatctc
      721 gatacctggc attgatatga taaaataccc atatcctttt tcagatataa agttattttt
      781 tctaccgtaa tgtatttcag cccggttgaa tatttctgag tgtgtaccga attaagaccg
      841 gggattttaa tctggattat tccctctcat gcggccaggt attcaggtgg gagaaaaatg
      901 attggtggac aggaatagtc aatggggcta cggtcagggc taaacaggaa ggcggggaaa
      961 ttattgtgga ctcatccctc gataaggatt tcattattgg gtatttcagg ctcgatgatg
     1021 atatcaaaca aatttaccaa tccatcaaca gggatgaaaa gatcggatca ttgatcaata
     1081 aatacaaagg gcttcgcatc atccggcagg atccgtggga atgtcttgta tcatatatgt
     1141 gctcaagcaa caacacgatc aggaatatca ccaattccat aaggcgtatg agtgaatgtt
     1201 tcggcaagga aatagaaaag ggtttatact cttttcccac accttttgca ctttcggatg
     1261 ttgaactttg cgacatgtca cagtgcaggc ttggttttcg ctccccgcgt attttgaaaa
     1321 tagcaaatgt tgtggcccgg ggcgaatttg acctttacgg cttaaaggac ttatcctaca
     1381 ggaaaggtcg cgaggaactt gttaaaatag aaggcgtggg aaataagatc gctgactgtg
     1441 tccttctttt ttcattcggg aagcttgaat cttttcccgt ggacacgcat atcgaacaga
     1501 taatggaaag atattacgga ttcaatttca aaggcgcaaa gagcaaaaag agggatgaaa
     1561 tagccgaatt tgcacggagt tattttggga tgtactgtgg atatgcccag gagtacctgt
     1621 atatggagga cctgggataa caatattaaa tgaagactat tactcagaca ggatctgtaa
     1681 taataatcca ttcatctttt aatgctcacc agatatggtt atccttaaat tataagaaag
     1741 tcaattaaag agtatgattc atatccttga aaatatcgat aagtggaagt gcgaatataa
     1801 gaaatgcggc gccaaatgct gttccgaagg tatccagctt acaattgagg atataaaaag
     1861 aataaaagaa ttgggatatt caccggatga tttccttgat tttgatgaca aaaataatat
     1921 attcaagata aagggaaaag aaggaaagtg cttttttaat ggaaaaaaac tggaatgcac
     1981 aatacgtgag aacgagccgc tagtctgccg tctacttcca ttcaggatca ttgaggtctc
     2041 atattcggat gaacctttga tgaagctaaa accagtcgtg gattgccccg gagtaggtca
     2101 gggcaaaaag atcgataaga aaaagatcga agcggatgca acaaagttcc tgcgggaaaa
     2161 ccagaaactc ataagggatg tcaggaagaa gggcatgaag gggattattg cagagcttta
     2221 attattttcc atgttttgca ggtttagttt gaatcgaaca cagatgacac ggatcagacg
     2281 gattttcacg gatccatggg cattacacta cttccatatc tttccattat agctgttcgt
     2341 aactgggcac cccaggcaac ggttagagat atgatgcgcg caatccttgc agttgcatcc
     2401 gttaccgcat ggcggcattt cgctaaattc aaaattcatt tttattggca tgaccaggtc
     2461 tttcatcgtt gctataacga tactgacctc ggcgcccctg accaggggat tgctcctgag
     2521 gtggccgtcc acgatcgcct caagtgttgc tatgtcctcg cccacgaaga aaaggcataa
     2581 attgtgtttt cctgaaacta tcaatccatt gaggaaatat gggcagtcct tgaaaatatc
     2641 aagaaccgac gaagtgttat tggcgatcac atcgaccttt gccatataaa ggccaacttt
     2701 tttcaggttc attccaacga catgtgataa ggcgcctttt tttttcaatt tataaattct
     2761 tgcgctaacg gagggctggg aaatcttgag tttttctgct atttcactct gggacatttc
     2821 aggatatttt tcaagaatag ataatatctc ccggtctctt ttgtccaggt caaggaattc
     2881 tttcatatat cctgatacaa acatgaatta ttaaatagtt ttctattaaa tactatgcaa
     2941 tactaaaata tccttattat ttataaccaa aatccttata agttataaaa acattcaata
     3001 tttgataata tatgaataga gatattgttt tcatagatga agaaaaatgt aacggttgtg
     3061 gtttgtgtat tcccaattgc gctgaagggg caattaaact gatcaacgga aaagcaaaac
     3121 tggttgacga caggttctgc gatggcctgg gcgcctgcct ggggcattgc ccccaggatg
     3181 cgatcaccat aacaaaaagg gatgcaaagg aatttgacga agaagcggta aaaaagcatc
     3241 ttaaggctga acccacaaaa gttatgaaaa aggcagaaaa gaatatccca tctggttgct
     3301 cgggaagcat ggccatggac ctgagaagtg agaaaaagtc tgatgccccg ggacggcaaa
     3361 gcgcacagct gaggcagtgg ccagtacaat tgacgcttgt atccccggaa gcgtcgtatt
     3421 ttaaggattc ggatcttctt gtagcggccg actgcgttcc cttcgcatat cctaatttcc
     3481 attcggattt tctcaaggga aaatctttga taatcggatg cccaaagctt gatgatacgg
     3541 atttctatat tgagaagttg accgaactca tcaagaaaaa caaccttaag agcatcacgc
     3601 ttgttaatat ggaagttccc tgttgtttcg ggcttcaaag aattgtcgag gaagcaattc
     3661 agaaggcggg gaaggccata cctatcaggc agacagtaat aacgatacgg ggagaaaaac
     3721 aataatagca tcgttatata aacttttcgg tttttattgg aatcagtgct atcttattgc
     3781 tacaatattc agaactaaac cataaaatgt gtatagttaa gtatagaatc catattgtaa
     3841 tcgaatattt cgagtgcaaa aatttcagta ctactttatt cacaccacac tatttatcag
     3901 tgctactaaa ttcataagaa tagtgtaaaa cattatatgt tttacagaaa ggttggggag
     3961 agacaaaaag caggttggag agtggggagt taattcataa gatagtttat tgaactccct
     4021 ctcgaattca tcatcgtgtg gaaagaatcc ctacttcaat ctatttagca gcatttccct
     4081 tgccatcctg gcatctgccc ttccctttgt ctttttcatc acagctccca caagcgagtt
     4141 aagcgccttt tccttcccgg acctgtaatc agccactgtc tggggatttt ccttgatggc
     4201 ttcttccgcc gcagttgaaa cgatatcgcc ttcaactttc agtagacctt tttctttcac
     4261 tatctcatca ggcgagccgc ctttatcaag cagcgtcctg ataatatcaa ccgcgccgtc
     4321 ttcggttatt ttcctgttca cgagtagatc gataacagaa agcatatgtt ctttctcgaa
     4381 agcctcgata gccaggtcac gataattcaa ttcccccttc aacacatcac atacccacac
     4441 cgcagccatt ttcggatcag ccttctttgc cacatcttca taaaagatcg caaacttgcg
     4501 ctccgatgtc agggcttttg catggtcatc cgtgatgccg tattgctcca caaatctcag
     4561 ccgtct
//
LOCUS       DATGBN010000333         9073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_259606, whole genome shotgun sequence.
ACCESSION   DATGBN010000333 DATGBN010000000
VERSION     DATGBN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259606"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VKL21_08140"
     CDS             complement(<1..241)
                     /locus_tag="VKL21_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMB45776.1"
                     /translation="MHIKEIIPLLETIALPSLSEDFDHGKIGLVLDRGSNIQKIAVAL
                     DPTDLVLKEAARMGADMLITHHTLIFDPINLISKRF"
     gene            507..1259
                     /locus_tag="VKL21_08145"
     CDS             507..1259
                     /locus_tag="VKL21_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45777.1"
                     /translation="MNKISIIRNLTTFILCIYLLAGPGSAVVYNGVDFPEGASSFADE
                     IVSYNPTTGVSGHDNASSALGIPDYINNTNYVSLGDEGTLVLKFTDNSLTTSGNENKD
                     LWIFEIGEKFESTDVYISKDGINWIYTGNTSNYTTGIDIDAYIGSEGAILGEQYSYVK
                     LADRLPHESDSPTAGADIDAIGAISSNTKVQAEADTGAISPDTTVPAGADINADRIIC
                     FRLSTIRNFLIIALSIAGLIGLMYLFKRRKGK"
     gene            complement(1299..2471)
                     /locus_tag="VKL21_08150"
     CDS             complement(1299..2471)
                     /locus_tag="VKL21_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMB45778.1"
                     /translation="MTISIALAGKPNSGKSTFFKAATLANVEIANYPFTTIDANHGVA
                     YVRTQCPCREFKLDCGNCREGVRFVPIEIIDVAGLVPDAHKGRGLGNEFLDNLRQAKA
                     IIHVIDASGTTDIEGNPVAAGSHDPLGDIEFLEHEITMWMAGILNRNWERLSRKVNAE
                     GLKIEETIASQLEGAGITEAHVRTILSRMRLVRDKPHVWTQEQIIELSGMLRAESKPL
                     IIAANKIDIASQENIEHLVKRGAIPVSGATEVVLRLADKSGAVKYIPGDADFVESQNL
                     SDAQKQALSKIRLLMKKNGGTGIQKCINETIFKLLDLIVVYPVEDENRLTDKKGNILP
                     DAFLMKKGSTPKDLAFMVHTDIGKSFLFAVDARTRMRLGEKHELKNGDIVKIVSVK"
     gene            complement(2519..2884)
                     /locus_tag="VKL21_08155"
     CDS             complement(2519..2884)
                     /locus_tag="VKL21_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMB45779.1"
                     /translation="MKEHKMSDVYNSILLDMKKLKGVKRIALAGRDGYLIGEHASEGS
                     ETLTLMSANMLQAAENITNTLEKVNPNRVIVDYKGGKLITTSVGSKALISVMTEKDAS
                     IDSIIKELDKTAGRIKEIL"
     gene            complement(2996..4021)
                     /locus_tag="VKL21_08160"
     CDS             complement(2996..4021)
                     /locus_tag="VKL21_08160"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HMB45780.1"
                     /translation="MRTIDWNDNKNDIVMIDQTLLPVEYKVIECATVDSLCEAIKSLR
                     VRGAPALGAAGGFGVAVAAFRSEARSMDKLLDDIQNAANIIIATRPTAVNLSWGVKRV
                     LGSLEDAKNLKEVRMFALDEAKAIADEDVAKNMQLGEHGAMLLDDGDTVMTHCNAGKL
                     ACVDWGTALGVIRSARAQGKDIKVISCETRPLNQGSRITTWELMEDKIPVTLITDSMS
                     GHVMRKGMIDKVIVGADRITQDAVFNKIGTYTHSVIAREHKVPFYVAAPVSTFDFERL
                     EDEIEIELRKPEELKYFGTYQIAPLGVNVYNPAFDATPMENVTALITENGVFYPPFLL
                     EEVKIKV"
     gene            complement(4188..4649)
                     /gene="ribC"
                     /locus_tag="VKL21_08165"
     CDS             complement(4188..4649)
                     /gene="ribC"
                     /locus_tag="VKL21_08165"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMB45781.1"
                     /translation="MPKIGIADTTFSRINMGKIAIDELRKNASIQIERYTVPGVKDLP
                     VACKILIEKKKCDIVMALGMPGSQPIDKMCAHEASQGIIQAQLMTNTHIIEVFVHEDE
                     GKDETQLAWLMEKRTREHAQNVIKLLFNKEQLEREAGTGQRQGFADVGSIR"
     gene            4777..6261
                     /locus_tag="VKL21_08170"
     CDS             4777..6261
                     /locus_tag="VKL21_08170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMB45782.1"
                     /translation="MQKKIAFLCVFVIAYTIYFIDLPPWWDGITTAMTALDTVKNNID
                     LYVDFFAKPPFVFLSLGILFKIFGYSPELIHIFMMIFSLGAIIFTYKVGEILAGKEIA
                     CSASFLLAFSPMFIAQSVNLNFDLPSMALLMATYYFLLKKNHIIAALSGIMLVMTKEI
                     GILFMAAALLSKILINYIGERKIGKAAITARIIPIIVFIIWAYGNYIRRGWFLFPRDS
                     PIIKLDSIFNENLTMRLEQLFVINFNWILTILILCSLILWAYRRHESFDREKMEMLLP
                     LILFSLFFFVVVAPIRDFNLPRYVIVLYPAFYLASAWGVSGLSGKNRKISKMILVSIF
                     LIFAAQTAYSIYSPDPYVFLDPATQEIYGANPDITLDGSGVMEINLKYVDYVKADIAL
                     VNYINTTEPFAPLLLNRFNHYGIFDAANKGIDIGYGQKSKRIYKELGEFYRSPGTISF
                     PAILVIEDFNRYDLEKLYTMYDVTLLKHIRVNGLGADVYEINKE"
     gene            6305..6721
                     /locus_tag="VKL21_08175"
     CDS             6305..6721
                     /locus_tag="VKL21_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMB45783.1"
                     /translation="MVKFRTIILGFIIMAIFSGCVSDTKSPGKTQYTDITVQQGKGMI
                     DRGEVFILDVRTQEEYTSGHINGSTLLAVQDIPKQELAEKLKEIPKDRKILVYCRSGR
                     RSAVASGILAENGFAQVYNMQGGITEWMNAGYEVEK"
     gene            6922..7248
                     /gene="ahaH"
                     /locus_tag="VKL21_08180"
     CDS             6922..7248
                     /gene="ahaH"
                     /locus_tag="VKL21_08180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase archaeal subunit H"
                     /protein_id="HMB45784.1"
                     /translation="MTRAEILSDIKQAEDEARSLVIQAHEARNKKVNEARSQARDILK
                     SAEEEAAQYFISELSKAKGESKIEKEKLIKKGYQEAEEIKTKARKNISYASKFILTEF
                     ESVANA"
     gene            7241..>9073
                     /locus_tag="VKL21_08185"
     CDS             7241..>9073
                     /locus_tag="VKL21_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HMB45785.1"
                     /translation="MLKPTRMTKVVIAGTKDQMEPAISVFHRLNVLHITDFSNETEDF
                     KIGRPLKTASKFSEYLLSLRAISNQLGLEESESGKITRSIELSSDIDEKITKLQKEVS
                     SRFDELRNIEFKLKEKEDLLSSVKPFFGLPLSLDSYTGYESIKVFTGYIDIDLESKII
                     KIHSNFELFMGEHEKRNVFALFIPKEFEREVQELLQEERTYVELKVPPLKGNPPIILN
                     ELTKEISNLKENISSIKTELDKLKEEYSEFIIAADEHLSIETQKSEAPLRFATSPNAF
                     IIDGWVPSNKFDEIEMELQKSTGGKVYFTRIAQEIDEKEIPIELENPVAAKPFELLIN
                     TFATPKYKEIDPSLFLFVTYPLFYALMLGDVGYGLIVSAIGFFIIRKFKTGGLNALAL
                     ILLISGLLSTVFGVIYGEFFGFPIFNLEFKGEFEAGILGIAGPLIAGIHLPVHRFESV
                     KPLLLLCFAIGIFHVILGYIIGFRNQVIMHDLKHAIYTKGSWMMILIGGVLAIAKIMP
                     ELMSKSPMPTGDPILMLGTGLFIIGAIILIIGEGFISILEMPTLLSNILSYSRILAIG
                     LSSAGIALAVNKMSMNLMIKPDGVLMGGGILLALAGVVVLFIGHV"
BASE COUNT         2698 a         1784 c         1761 g         2830 t
ORIGIN      
        1 aaaatctttt cgatataaga tttatggggt caaatatcag ggtgtgatgt gttatcagca
       61 tatctgcgcc catacgtgct gcttctttaa ggacaaggtc agttggatca agtgcaactg
      121 caatcttttg gatattgctg cccctgtcaa gaacgagccc tatttttccg tgatcaaaat
      181 cttctgaaag tgagggcaga gcgatggttt caaggagtgg gataatttct ttgatatgca
      241 tggttgtttc ttggttggag ggatatttaa gagttggttc ttaggagact cttacaatta
      301 attgacatga tttttatttc tcttggttac tttgttggaa cctataagct attaagtggt
      361 atggtgatta tcattattat ttacagataa atgagaatat ttatatagta atagactatt
      421 tttacattac atgaaagtat cctgaaaaag gttatatatc tgaaactaag ttcaaaattt
      481 ccaaaaaaaa ttaaaagagg tatgaaatga ataaaatatc gataattaga aatttaacta
      541 ctttcatcct gtgtatctat ttgttagcag gtccgggtag cgcagtagta tataatggag
      601 ttgattttcc ggaaggtgca agttctttcg cagatgagat tgttagttat aatccaacaa
      661 ctggcgtttc gggtcatgat aatgcatcaa gtgccttagg tattccggat tatatcaata
      721 atacgaatta tgtttctttg ggtgatgagg ggactttggt attaaagttt acagataact
      781 ccctgacaac ctcaggaaat gaaaataagg atttgtggat ttttgaaatt ggggagaaat
      841 ttgaatcaac agatgtttat ataagcaaag atggaataaa ttggatatac acaggtaaca
      901 catccaatta tacaacaggt atcgatatcg atgcatacat aggctctgaa ggagccattt
      961 taggtgaaca atatagttat gtaaaacttg cggacagatt gccgcacgag agcgattctc
     1021 ctaccgcggg agcagatata gatgctattg gagccataag ttctaataca aaagtacaag
     1081 cggaagcaga taccggagct ataagtcctg atacaactgt acctgcggga gcagatataa
     1141 atgctgatag aattatatgt ttcaggctgt caacaatacg aaattttcta ataatagcat
     1201 tgtcaattgc aggtttaatc ggtcttatgt atcttttcaa gagaagaaaa ggcaaatagg
     1261 tgtcttttct tttttaattt tctatcctgt ttcaactgct atttcacaga cactatcttc
     1321 actatatccc cattcttcaa ctcgtgtttc tccccaagcc tcatcctggt gcgagcatcc
     1381 actgcaaaaa gaaaactctt tccgatatca gtatgcacca tgaaagcaag gtcttttggc
     1441 gtgctaccct ttttcatcag aaatgcatcg ggaagtatgt tacctttctt atccgtcaac
     1501 ctgttctcat cctctacagg atagaccact atgaggtcaa gaagcttaaa aatcgtttca
     1561 ttaatacatt tctggatacc tgtcccgcca tttttcttca ttagcaggcg aatctttgat
     1621 agcgcctgct tttgtgcatc ggaaaggttt tgcgactcaa caaaatcagc atcaccggga
     1681 atgtatttta cggcgccgct cttatctgca aggcgaagca ctacttccgt tgcgccgctg
     1741 accgggattg cgcccctttt caccaggtgt tcgatattct cctgtgaagc aatatcgatt
     1801 ttatttgccg caattattaa cggtttactt tcagccctca gcataccgga aagttctatt
     1861 atctgttctt gcgtccatac atgtggttta tctcgcacaa gtctcattct cgaaagaatt
     1921 gttctaacgt gggcttcagt tattcctgcg ccttcgagct gactggcaat tgtctcttcg
     1981 atctttaaac cctcagcatt cacctttctt gacagcctct cccagtttcg gttcaggatc
     2041 cccgccatcc acatggttat ctcatgctca aggaattcaa tgtcgccaag tgggtcatgc
     2101 gaccctgcag ctacgggatt accttcaata tccgttgtgc ccgatgcatc tattacatgg
     2161 attattgcct ttgcctgtct taaattatca aggaattcat tgccaagccc ccgtccttta
     2221 tgtgcatcgg ggacaagccc ggcaacatct ataatttcaa taggtacgaa cctgacccct
     2281 tcccggcaat tcccgcaatc aagtttaaat tccctgcagg ggcattgcgt tctcacatat
     2341 gccaccccgt ggtttgcatc gatagtcgta aaaggataat tagcgatctc aacattggca
     2401 agtgttgcgg ctttgaaaaa cgttgatttc cctgagtttg gtttcccagc aagagctatt
     2461 gaaatggtca tgctgctaat atcatttaat taaacttaac tttaatcttt gctcaaaact
     2521 aaaggatttc ctttatcctt cctgctgttt tatcaagttc tttaatgata gagtcaatac
     2581 ttgcatcctt ttcagtcata accgaaatca gtgcttttga acccactgaa gttgtaatga
     2641 gctttccgcc cttataatca actattacgc gattgggatt aactttttca agcgtattag
     2701 tgatattttc agcagcctgt aacatgttcg ctgacatcag agtcagcgtt tccgaccctt
     2761 cgcttgcatg ttcaccgatc aggtagccat cacgacctgc aagtgcaatc cttttcactc
     2821 cctttagttt cttcatgtcc aacagtatac tattatatac atccgacatt ttgtgctctt
     2881 tcatacatta ccccaataaa ttataaacct gcgatgttat aattctttcg ggtctttcaa
     2941 aaattattca tttttataaa aatatttttt gtaccatatt atgtcacaag aaaaattaaa
     3001 cttttatctt gacttcctca agaagaaatg gtggataaaa taccccattt tctgtgatca
     3061 atgcagtcac attttccatg ggggttgcat caaaggcggg attataaaca tttactccaa
     3121 gtggagctat ctgatacgtt ccaaaatatt ttagttcttc gggttttctt aattcgatct
     3181 ctatctcatc ttcaagtctt tcaaaatcaa acgtggaaac aggcgcagca acataaaacg
     3241 gcaccttgtg ctctcttgcg atgaccgaat gtgtataagt cccgatctta ttgaacacgg
     3301 catcctgggt gatcctgtca gcgcccacga tgactttatc tatcattcct tttctcatca
     3361 catgaccgga catactgtcg gttataagag taaccggaat tttatcttcc atcaattccc
     3421 atgttgttat tcggcttccc tgattgagcg gacgcgtctc gcaagaaatt acttttatgt
     3481 cttttccctg ggcgcgggca gaacggataa cccccagggc agtgccccag tccacacatg
     3541 caagctttcc agcattgcag tgcgtcatga cagtatcccc gtcatcaaga agcatggcgc
     3601 catgctctcc aagctgcatg ttctttgcca catcctcatc agcgatcgct tttgcctcat
     3661 caagcgcgaa catccttact tctttgaggt tttttgcatc ttctaatgac cccaatacgc
     3721 gttttactcc ccatgaaagg tttactgccg tgggtctcgt agcgattatg atgtttgcgg
     3781 cattttgaat atcatccagt aacttatcca tggatctggc ctcacttcga aaagcagcaa
     3841 cagcaacgcc aaatcctcct gctgcgccaa gcgcaggcgc gccgcgaacg cgaagggatt
     3901 taatggcttc gcatagtgaa tctaccgtgg cgcattctat gaccttatat tccacaggta
     3961 aaagggtttg atctatcatt actatatcgt ttttattatc attccagtct attgttctca
     4021 ttgaaatcaa tttgtatcat gaggccttgt attaaaatac tgccgtttaa tttgagaaac
     4081 caccgggaga gcagagtata cagaggaaag tttcaaagct ctgtgctcat acattaattt
     4141 tatactaatc cccactcccc aacctgtcta tcattttgtt catattatta tcttatcgaa
     4201 ccaacatcag caaaaccctg cctttgcccc gtaccagctt cacgctctaa ttgctcttta
     4261 ttgaaaagga gtttgataac attctgtgca tgttcacggg tgcgtttttc catcagccat
     4321 gcaagctgcg tttcatcctt tccttcatct tcatgcacaa agacctcgat tatatgcgtg
     4381 ttcgtcatca actgggcctg gattatcccc tgtgaggcct catgagcgca catcttgtca
     4441 attggctggg atcctggcat gccaagcgcc atcactatat cgcatttttt cttttcgatc
     4501 aatattttgc atgccacagg aaggtctttt actccgggaa ccgtatagcg ctctatctgg
     4561 atcgatgcat tttttcgcaa ttcatcgatt gcgattttcc ccatatttat cctggaaaat
     4621 gttgtatcgg caattcctat tttaggcata tctattcaat caaaatagct tttaacatat
     4681 attaaatgat ttcattttat agcttcctgg aattaaacct aagcgatgac ttacttataa
     4741 ataatatctt gttgtagaag tccagggacg gccgatatgc aaaaaaaaat tgctttctta
     4801 tgtgtattcg taatagcata cacaatatat ttcatcgacc tgcctccctg gtgggatggc
     4861 attacaacag ccatgactgc acttgatact gtgaaaaaca atattgacct ctatgttgat
     4921 ttttttgcca aacccccatt cgtttttctc tcccttggca tattattcaa aatatttgga
     4981 tattcgcctg aattgatcca catattcatg atgatattct ctcttggagc gatcatcttt
     5041 acatacaaag tcggagagat tcttgcaggc aaggaaatag catgttctgc atcgtttctc
     5101 cttgcgtttt cccccatgtt catagctcaa tcggtaaatc tgaactttga ccttccaagc
     5161 atggctcttt tgatggcaac atattatttc ctgttaaaga agaaccatat tattgcggcc
     5221 ctttccggta taatgcttgt gatgacaaaa gaaatcggga ttttattcat ggcggctgca
     5281 ttactcagta agattttgat caattatata ggagaaagaa aaattggaaa agctgcaata
     5341 acagcccgga tcatcccgat cattgtattc attatatggg catacggtaa ctacataaga
     5401 aggggctggt ttctcttccc gagggattct ccaattatca agttagattc aatatttaat
     5461 gaaaatctca ctatgcgtct tgagcagtta ttcgtgatca acttcaattg gattttgaca
     5521 attctgatct tatgttctct aatcctgtgg gcttacaggc ggcatgaaag ctttgatcgt
     5581 gaaaaaatgg aaatgctatt accattaata cttttttccc tctttttttt cgtggtggtg
     5641 gcgcctataa gggattttaa ccttcccagg tatgtaattg ttttatatcc ggcattctat
     5701 cttgcctcgg cctggggagt ttccggatta tcgggaaaga accggaaaat atcaaagatg
     5761 atcctggttt ccatattttt gatatttgca gcacaaaccg catatagcat ttacagccct
     5821 gatccttatg tttttcttga ccccgccaca caggaaatat atggtgcgaa tcccgatatt
     5881 accctggatg gttcaggtgt aatggagatc aacctgaaat atgtggatta tgtaaaagca
     5941 gatatcgcac ttgtaaatta tatcaataca acggagcctt tcgccccttt attattgaac
     6001 cgtttcaatc attacggtat ttttgacgca gccaataaag gaatagatat cggatacggc
     6061 cagaagtcca aaagaatata taaagaactt ggtgagtttt acaggtcgcc tggaacgatc
     6121 tcatttcctg ctattcttgt tattgaagat ttcaatagat atgaccttga aaaactttat
     6181 actatgtatg atgtgacttt gttgaaacat atccgggtaa atggtctggg tgcagatgtt
     6241 tatgagataa acaaagaata ataacccaaa ttttttaaag cgggacggaa ataatgaata
     6301 tattatggta aaattcagaa caattatatt gggcttcata atcatggcaa tattttcagg
     6361 atgtgtaagc gatactaaat cacccggaaa aacacaatac acagacatca ctgtccagca
     6421 gggaaaagga atgatcgaca gaggagaggt ttttattctt gatgtaagga cacaggaaga
     6481 atatacatcc ggacatatca atggttcaac attgctggca gtgcaggata taccaaaaca
     6541 ggagcttgcc gaaaagctaa aggaaatccc gaaggacagg aaaatccttg tttattgcag
     6601 aagcggtcgt cgtagtgccg tggctagtgg aatacttgct gaaaatggat ttgcgcaagt
     6661 ttacaatatg cagggtggga tcacagagtg gatgaatgca ggttatgaag tagaaaagtg
     6721 aaacatgata aatcttcttt ttaatttata tatttattat acagaaaatg cataacggcg
     6781 aacatttaaa acgatttttc attaaaaata gtagtgcata accgcattat ctaaaataaa
     6841 accgaataat ttaagtaggt agtctcctat gcagaatcaa attcaaaacg agattattat
     6901 tttgcgtcac agaggaatta aatgacaaga gctgaaattt tatctgatat caaacaggct
     6961 gaagatgagg ccaggagtct ggtaatccag gcacatgaag ccagaaataa aaaagtaaat
     7021 gaagcaagat cccaggccag ggatatcttg aaatctgcag aagaagaagc agctcaatat
     7081 tttatatccg aactcagcaa agcaaaaggc gaaagtaaga tagaaaagga aaaattgatc
     7141 aagaaaggat atcaggaagc agaagagatc aaaacaaagg cacgaaaaaa tatttcatat
     7201 gccagtaaat ttattttaac tgagtttgag agtgtggcga atgcttaaac ccacaagaat
     7261 gacaaaggtc gtaatagcag gtacaaaaga ccagatggaa cctgcaattt cagtatttca
     7321 caggttgaat gttcttcata taactgattt ctcaaatgaa accgaagatt ttaagattgg
     7381 ccgcccatta aaaacagcat ccaaattctc agaatatcta ctttccctcc gggcgatttc
     7441 taaccagctg ggactggagg aaagcgaatc cgggaaaata accagatcaa tagaattatc
     7501 ttcagatatc gatgaaaaaa tcacaaaact tcaaaaagag gtttcatccc ggtttgatga
     7561 gttaagaaat attgagttca agcttaaaga aaaagaagac ctcctgtcct cggtcaaacc
     7621 attctttggt ctgcctctgt ctttggattc ttataccgga tatgagagta taaaagtatt
     7681 tacgggctat atagatattg atcttgaatc caaaattata aaaattcaca gcaattttga
     7741 attatttatg ggagaacatg aaaaacgaaa tgtctttgcc ctctttattc caaaagaatt
     7801 tgaaagagaa gttcaagaac ttctccagga agagcggaca tatgtggaat taaaagttcc
     7861 cccgttaaaa ggtaatccgc caatcatatt gaatgaactc acaaaagaga tttcaaatct
     7921 taaggaaaat atctcttcaa taaaaacgga acttgataag ctaaaggaag aatattccga
     7981 attcataatt gcagcggatg aacatctttc aattgaaacc cagaagtctg aagctccttt
     8041 aagatttgct acgagtccaa atgcatttat catagatggc tgggttccat cgaataaatt
     8101 tgatgaaatt gaaatggaat tgcagaaaag cacaggtgga aaagtatatt ttaccaggat
     8161 tgcccaggaa atcgatgaaa aagaaatccc tatagagctt gaaaatcctg tagctgcaaa
     8221 accgtttgaa cttttaatta atacgtttgc aacgccaaaa tataaagaga tcgatccatc
     8281 cttattttta ttcgttactt atccgttgtt ttacgccctt atgctggggg atgtaggata
     8341 tggattgatc gtgtctgcaa taggcttttt tataatacgg aaatttaaaa ccggaggact
     8401 caatgcttta gcgttgatat tattgatttc aggattactt tcaacagttt ttggtgtaat
     8461 ttatggggaa ttcttcggat tccctatatt taatctggaa ttcaaggggg aatttgaagc
     8521 aggaatcctg ggtattgcag ggcctctaat agcaggaata catttacctg ttcatcgttt
     8581 tgagtcggtc aaaccattac ttcttctgtg tttcgctatt ggaatatttc atgttattct
     8641 cggatatatc atcggattca gaaatcaggt aataatgcat gatctgaagc atgcgatcta
     8701 tacaaaggga agctggatga tgatattgat tggcggagtt ctggcaatag caaagataat
     8761 gcctgaattg atgtcaaaat ctcccatgcc aacaggagac ccgattttga tgttagggac
     8821 aggattattc atcattggag cgatcatatt aataatagga gagggcttta tttcaattct
     8881 tgagatgcca acacttctta gcaatatatt atcttactca aggatacttg caattggact
     8941 ttcttctgcg ggaatagcgc ttgcagtaaa taagatgtca atgaatctta tgattaaacc
     9001 ggacggtgtt ttaatgggtg gaggaatact acttgccctt gcaggggtcg tagttttgtt
     9061 catagggcat gtg
//
LOCUS       DATGBN010000334         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_531234, whole genome shotgun sequence.
ACCESSION   DATGBN010000334 DATGBN010000000
VERSION     DATGBN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531234"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VKL21_08190"
     CDS             <1..288
                     /locus_tag="VKL21_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB45786.1"
                     /translation="IHDPEIIFWDEPTTGLDPQTRQAIWRLAMKFNKEGKTLIFTTHY
                     MEEADKLCNRVAIMNAGKLVAIDTPENLKKTSNSTSLEEVFIHLTGEEIRD"
     gene            290..1402
                     /locus_tag="VKL21_08195"
     CDS             290..1402
                     /locus_tag="VKL21_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45787.1"
                     /translation="MNIKRELKNSRIIMIKEFKQLFRKKVLFVPLFIFPIVMIVFFGY
                     GMGGSVNNVGMLIVNNDMGVSSNALIQELGTFIPKNSDNKMFEITYTKDMAQSEAEDK
                     INSGQFKAVLIIPADYSNSLAKNEKPEVILLTDQSDTTSSGIIIETMNRILSESQPGT
                     ILLVVPDIYGNLKYIDFLTPAVIALTVFFGTVMSTGYAVAGEREDGTIVRILMTPISR
                     GAVILGKTIHQLILQLARAVILILAAILIFGVKMNGSWFLVALILIIFTFGAVGMGIA
                     ISAKSEDMTSFQQISMLFTLPSMFATGVFFPLDSVPDWMRWIAYFIPLTYANDAMRIV
                     MIKGQGINAIAGDLIILLIFSIIIFAVAIRIFRREV"
     gene            1403..2389
                     /locus_tag="VKL21_08200"
     CDS             1403..2389
                     /locus_tag="VKL21_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45788.1"
                     /translation="MNKINKLIFFYLVTITIASAGYDTPAMYNVPRSFDFAKNYYNVY
                     GGPDINATIIGSNEFERGQTATLNIDLMNRGKFLGFEADEDPTGANEIFAAQTEIKVE
                     GKIVDAIGIVASLSADTGSPVEVKSVSQQVGSIRSGQNALAPLKFDIKIDKKARAGTY
                     PLSLDVTYDYQKNVQIIDANATQETYDANYWYGIMAQNQTMTIRVKKQADFEITKTNG
                     SLFPGGEDIIEITIKNTGEEKARDVRAIINPSDPISTTDDKAFLYNIEPDEVVVAKYR
                     IKADSKAVSKFYSMDIVLRYETPEGDIRYSSNLLAPVEVKEIGFFQRFFGWI"
     gene            complement(2430..>3230)
                     /gene="mptA"
                     /locus_tag="VKL21_08205"
     CDS             complement(2430..>3230)
                     /gene="mptA"
                     /locus_tag="VKL21_08205"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HMB45789.1"
                     /translation="DIFVDLPSDRKGANLSRNFEAIDEVLEEMIASPVYEIEDLCSEV
                     AKRLIDRHEYALIAEVRMKSEYVLKRETPATKLNCQEVVNIFAEAKAIRGKILTIRKL
                     VGAEVLGITACPCAQEIMVDKARKELKMLGVEFEVIRKFLNNVPMPTHNQRGRGIISI
                     EVQNSHFVSLEKIINIIEHSMSSQMFELLKRTDEAMIVERAHKNPKFVEDCVRTMAQK
                     VVKEFPELPDDSIIVIKQINEESIHRHNAFAERKSTLGELRQEINNIG"
BASE COUNT          961 a          671 c          699 g          899 t
ORIGIN      
        1 atacacgatc ctgaaataat cttctgggat gaacctacga cgggtcttga tccacagacc
       61 aggcaggcta tctggagact tgcaatgaag ttcaataaag aaggtaaaac actgatcttc
      121 actacacatt atatggaaga agctgataaa ttgtgcaaca gggttgcgat aatgaatgcc
      181 gggaaactgg tggcgattga tacaccggaa aacctgaaaa aaacgtcaaa tagcacaagc
      241 cttgaagaag tctttatcca tcttacaggc gaggagatac gtgattgaaa tgaacattaa
      301 aagggagctc aaaaattcaa ggataataat gataaaggaa tttaagcaac ttttcaggaa
      361 aaaagtcctc tttgtgccat tattcatatt cccaatagtg atgatcgttt ttttcgggta
      421 cggtatgggg ggaagcgtca ataacgtagg catgctcatc gtgaacaatg atatgggcgt
      481 gagttcaaat gcactgattc aggagcttgg gactttcatt cccaagaaca gcgataacaa
      541 gatgtttgag ataacatata caaaggacat ggcgcaatcc gaagctgaag ataaaattaa
      601 ttcagggcaa tttaaggcgg ttttgatcat acctgcggat tatagcaaca gtcttgctaa
      661 aaatgagaag cctgaagtca ttctgctgac cgaccagtcg gatacaacat caagtggtat
      721 tataattgag accatgaaca ggatactttc agaaagccag ccgggaacta ttcttttggt
      781 agtcccggat atttatggaa atctgaaata catagatttt cttacacctg cggtaatagc
      841 cctgaccgta ttcttcggta ccgtgatgag tactggctat gcggttgcag gtgaaaggga
      901 ggatggcacc atagtccgaa tccttatgac gcctatcagc aggggtgcag tcattttagg
      961 aaagacaatt caccagctta tccttcagct tgctcgagcc gttatcttga tccttgccgc
     1021 aatacttatc tttggcgtca agatgaatgg atcctggttc cttgttgcac tgatacttat
     1081 tatatttact ttcggggcag tggggatggg gatagccata tctgcaaaat cagaagacat
     1141 gacatcattc cagcagataa gtatgctctt cacgcttcca tccatgttcg ccacaggcgt
     1201 atttttccct ttggactcag ttcctgactg gatgcgctgg atagcatatt ttatcccgct
     1261 tacttatgcg aatgatgcca tgcgtattgt catgataaaa gggcagggta taaatgcaat
     1321 agctggtgac ctgataattc tacttatatt ctctataatt atctttgctg ttgccattcg
     1381 tattttcagg agggaagtat aaatgaataa aattaataaa ttaatatttt tttatttggt
     1441 gacaataaca attgcatcag caggttatga tacgcctgcc atgtacaatg tgccaagaag
     1501 ctttgatttc gcaaagaatt attataatgt atacggaggt ccggatatta atgcgaccat
     1561 tatcggaagt aatgaatttg aacgggggca gaccgctacg ctaaatatcg atcttatgaa
     1621 caggggaaaa tttctcggat ttgaagctga cgaggatcca acaggtgcca atgaaatctt
     1681 tgcagcccag acagaaataa aagttgaggg aaagatagta gatgcaatag gtatagtcgc
     1741 atcgttatca gctgatacgg gaagcccggt cgaagtcaaa tcagtgagcc agcaggtagg
     1801 ttcaataaga agcgggcaga atgcacttgc cccattgaaa tttgatatca aaatagacaa
     1861 gaaggccagg gctggtacat atcctctttc gctggatgtc acttacgatt accagaaaaa
     1921 cgtacagata atcgatgcaa atgcaaccca ggagacctat gatgcgaact actggtatgg
     1981 cataatggcg caaaaccaga caatgaccat tagagttaag aagcaggctg attttgagat
     2041 aacaaaaacc aatggaagcc tcttcccagg gggcgaagac attattgaga taacgatcaa
     2101 gaataccggg gaagaaaaag caagagatgt aagggcaata atcaatccct ctgatcctat
     2161 aagcacgaca gatgacaaag cattcctgta caacattgaa cccgatgaag ttgttgtggc
     2221 gaaatatagg ataaaagcag atagcaaagc agtctcaaaa ttctactcta tggatattgt
     2281 gctgcggtat gaaaccccgg aaggcgatat cagatactcc tcaaatttac tggcgcctgt
     2341 tgaggtgaag gagataggat ttttccagag atttttcgga tggatttagg atacctcaaa
     2401 atcgcatcaa ctgatcttgc cgaatcatct cacccgatat tattgatctc ctgcctcaat
     2461 tcccccaatg tggatttcct ctccgcgaac gcattatgcc tgtggatgct ttcttcattt
     2521 atctgtttta tgacaataat agaatcatcg ggcagctcag gaaattcctt gactactttt
     2581 tgggccatcg tgcgcacgca atcttccaca aattttggat tcttgtgcgc cctttcaacg
     2641 atcatcgctt catcagtgcg ctttagcaat tcgaacatct gcgaactcat ggaatgctcg
     2701 atgatattga tgatcttttc aagcgagaca aaatgactgt tctggacctc tatcgaaatt
     2761 ataccccgtc ctctctggtt atgcgtaggc atgggcacat tattcaagaa tttcctgatg
     2821 acttcgaatt ccacacccag catctttaat tcctttctgg ccttatccac cattatttcc
     2881 tgggcgcagg ggcatgcagt gatgccaaga acttcagcgc ctacaagttt tcttattgta
     2941 aggattttac cccggatagc ctttgcctcc gcgaaaatat tcaccacttc ctgacagttt
     3001 aattttgtcg ctggtgtttc ccttttcagt acatattcgc ttttcatcct tacttcggcg
     3061 atcagggcat attcatgcct gtcaataagc ctttttgcca cctcgctgca tagatcctcg
     3121 atctcgtaaa caggagatgc tatcatttcc tccagcactt cgtctatggc ctcaaaattc
     3181 ctcgatagat tcgcgccttt cctgtcggaa ggaagatcca caaaaatatc
//
LOCUS       DATGBN010000335         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_337164, whole genome shotgun sequence.
ACCESSION   DATGBN010000335 DATGBN010000000
VERSION     DATGBN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337164"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VKL21_08210"
     CDS             <1..209
                     /locus_tag="VKL21_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HMB45790.1"
                     /translation="EHELERLRNEHCERIDNECCLHIRFDKQAAFKGKVNLAKTTDTI
                     TAKIKLKVYPANYEKALEAANRLF"
     gene            275..350
                     /locus_tag="VKL21_08215"
     tRNA            275..350
                     /locus_tag="VKL21_08215"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:His,seq:gtg)
     gene            complement(375..1082)
                     /locus_tag="VKL21_08220"
     CDS             complement(375..1082)
                     /locus_tag="VKL21_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HMB45791.1"
                     /translation="MVVADMISIITPVFNEEKNIKPYISHLGSLEGDFELILVDGGSS
                     DRTVEEVQKCIDSFNHKLLLLISERGRAIQMNKGAKIAQGDILLFLHVDCVLDKDALT
                     IMEQVTQDNNIAGGGFKQAFSNPGFFLKFQSDIGNIRAGLTQIFFGDYGIFIKKKVFR
                     KIGGYDDIPFLEDVEICRKAKRFGRLLQVDRRIVTSPRRYLQKGKVRLTIAFILANMF
                     NEIGWRPGFLRKYIVEM"
     gene            1135..1800
                     /locus_tag="VKL21_08225"
     CDS             1135..1800
                     /locus_tag="VKL21_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45792.1"
                     /translation="MGSLKIDVDKVMTAGNGILIMGIAWLLFWLGPAFFLFREDPRWG
                     HNFAIPLTFITAGLASNTRIISCQLVAVTSAFFLVPTLLAYWSWAVATMISGGLLGFF
                     LILYIIETRRETELIVPNPRLRTWLKIHLLTFAYLGLAHMPLIFYLVRWTNPDAFAQF
                     LPMEHEISTSVFNAMLIPLVILAILERFVKRVGIFQVAKAGFAWVVLMMVIPLISINL
                     LGQ"
BASE COUNT          576 a          426 c          393 g          629 t
ORIGIN      
        1 cggaacatga acttgaaagg ctaagaaatg aacactgtga aagaattgat aatgaatgtt
       61 gcctgcatat aagattcgat aagcaggccg cttttaaagg aaaagtcaat cttgcaaaga
      121 ctaccgatac tatcaccgca aaaataaagc ttaaagtata tccggctaat tatgaaaaag
      181 cgcttgaagc tgctaataga cttttttaat atcaaacaaa acctttatct ttcactgata
      241 catttacggg gtacccccat caattaaaca aggcgcctgg gtggggtagc tggttatcct
      301 atgggactgt ggatcctacg actcgggttc gaatcccgac ccgggcccca aattttttgt
      361 tcgagtttat tatttcacat ttcaacaata tatttccgga ggaatccggg acgccaccct
      421 atctcgttga acatatttgc cagaataaat gcgattgtca acctgacttt acctttttgt
      481 aaataccgcc tgggtgaggt cactattctt ctatcgacct gcaaaagccg gccaaatctt
      541 ttggctttcc tgcaaatctc aacgtcttca agaaaaggga tatcatcata acctccaatc
      601 ttccggaaaa ctttcttttt aatgaatatc ccataatctc caaaaaagat ttgagttaga
      661 cctgctcgaa tattaccgat atcgctctgg aatttcaaga aaaagccagg attggaaaat
      721 gcctgtttga aaccccctcc tgcgatatta ttatcttggg taacttgctc cataattgtc
      781 aaagcatcct tatcgagaac gcagtcaaca tgaagaaaaa ggagaatatc accctgtgca
      841 attttcgctc ctttattcat ctggatcgct cttccacgct cagatatcag caagagaagt
      901 ttgtgattga agctatcaat gcacttttgc acttcctcca ctgttcggtc actgctcccg
      961 ccatccacta atatgagttc aaaatctccc tccagcgatc caagatgaga tatgtagggt
     1021 ttaatatttt tttcttcatt gaatactgga gttattatgg aaatcatatc tgcaacaacc
     1081 atttaataat atgtcagaaa ctttcatata ttttgaggtc aagttctaaa tattgtggga
     1141 tctttaaaaa ttgatgttga caaggtgatg accgcgggca acggtattct tattatgggc
     1201 atcgcatggc ttttattctg gcttggccct gctttttttc ttttcaggga agaccccagg
     1261 tggggccata atttcgctat tcccttaact tttattacag caggtcttgc ttcaaataca
     1321 aggatcattt catgccagtt agtagcggtc acaagtgcct ttttcctcgt acccacgctt
     1381 ctggcctact ggtcatgggc tgttgccaca atgatctcag gagggctact gggttttttt
     1441 ttgatattgt atattattga aaccaggaga gaaacggaac tgatagttcc aaacccaagg
     1501 ttaagaacat ggcttaagat ccatctactg acatttgctt atcttggtct tgctcacatg
     1561 ccgctaatat tctatctcgt aagatggaca aatccagatg ctttcgcgca atttcttcct
     1621 atggaacatg aaatttctac aagtgtcttc aatgccatgc ttatacctct ggttatcctt
     1681 gccatacttg aaagattcgt aaagagggta gggattttcc aggtcgcaaa ggcaggattt
     1741 gcctgggttg ttcttatgat ggttattccc ctcatttcca ttaacttgct cggccaatga
     1801 actatttctt aagaaatgtg aaatatatga agatgtgcgt cttatgcgta ctatggggtg
     1861 ttgaatgaat aagaaagatc cagcaataat gtacggctgc tttcatcctg accatattga
     1921 agagatgaaa tattttgaag aatgtaaggt ctatactgtc cagatagaat cgaatctggc
     1981 ctgtccccag ggatgcctct attgttatgc tgcctcagaa gaaa
//
LOCUS       DATGBN010000336         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_143096, whole genome shotgun sequence.
ACCESSION   DATGBN010000336 DATGBN010000000
VERSION     DATGBN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143096"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VKL21_08230"
     CDS             <1..200
                     /locus_tag="VKL21_08230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMB45793.1"
                     /translation="SSVGKVYPFEVFIKKDIANVSSDSKVKANQIRTLDKKRMKERIG
                     IIPDEILKKIEKAVKIHLEIE"
     gene            318..761
                     /locus_tag="VKL21_08235"
     CDS             318..761
                     /locus_tag="VKL21_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45794.1"
                     /translation="MTDIRRRVEEDRGMLKKIESFLPGFKGYRKREDLRAADSLLRQQ
                     LAMRMGEINRKFDNCRDELTKALEIGLISEIGDITKMSRQVENKIRHAEQGYSNISAD
                     IRFEEQELNRMYEWDISLLQTIEYSRFGRKRSGIDHFHVGRFGKR"
     gene            658..1482
                     /locus_tag="VKL21_08240"
     CDS             658..1482
                     /locus_tag="VKL21_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMB45795.1"
                     /translation="MNGIFRFFKQLNTAGSGGSVLGSTTFMWDDSAKGENVLFRIPRN
                     IIWNDNVVVREDESAVFFRDGKVLTVLDRPGRFGLSTLNIPVLTELQRIVTGIQPVAE
                     VYYVQRRELRGKFGSSEAMTFRDQDFGLVRLRIFGQFAYKKAIDTRSGIGALGLTDQK
                     QKEAYLAFQAGGAMRDAAKQEGGAAGAGAGAGVGLGAGMGMGMIMASQLGQTMQPQQT
                     RPNGIKCPSCSADLPSGSKFCTNCGNKIGPGNMCPKCNIELTPGAKFCPGCGAKIE"
     gene            1495..>1778
                     /locus_tag="VKL21_08245"
     CDS             1495..>1778
                     /locus_tag="VKL21_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45796.1"
                     /translation="MEIKCPQCGGDVKVIEGETFLTCEYCSSAIYIDKSKVVFHYLIK
                     PTFDEAGALASLRRWMAGSSTVKGLDKEARITKTEFVFFPIWYFKIRQDE"
BASE COUNT          555 a          324 c          480 g          419 t
ORIGIN      
        1 cttcaagtgt agggaaagtg tatccttttg aagttttcat aaagaaagat attgcaaatg
       61 tatcaagcga ttctaaggtc aaggcaaatc aaataagaac attggataaa aagaggatga
      121 aagagcgaat agggataatt cctgatgaga tattgaaaaa gatagagaaa gctgtgaaga
      181 tccaccttga gattgaataa aatcctcaga aacgtttgcg cacctatatt cacatttttt
      241 atagaagaat ttattaccaa taaaattact tctgcctcaa attcgttgat gattataatt
      301 ttataagggt gaataaaatg actgatatcc gaagaagagt ggaagaagat aggggaatgt
      361 taaagaagat tgaatcattc cttcctggtt ttaaggggta ccgcaagcgt gaggacttaa
      421 gggcggctga tagcctgttg aggcagcagc ttgccatgcg gatgggagaa ataaaccgca
      481 aattcgataa ttgcagggat gaactcacaa aagcccttga aatcgggctc atttcagaaa
      541 taggagatat tacgaagatg tcaaggcagg ttgaaaataa gatccggcat gcagagcaag
      601 ggtattcaaa tatttcggcg gatatccgat ttgaggaaca agagttgaac aggatgtatg
      661 aatgggatat ttcgcttctt caaacaattg aatacagcag gttcgggagg aagcgttctg
      721 ggatcgacca ctttcatgtg ggacgattcg gcaaaaggtg aaaatgtcct gttcaggata
      781 cccaggaaca ttatctggaa cgacaatgta gttgtcaggg aagatgaatc ggccgtgttc
      841 ttcagggatg gaaaagtact cacggttctt gaccgaccgg gaaggttcgg gctctcaacg
      901 cttaacattc ccgttctcac agaactccag aggatagtta ccggcataca gccagtagca
      961 gaagtatatt atgtccagcg ccgggaactg agaggcaagt tcggaagctc tgaagcaatg
     1021 accttcaggg atcaggattt tggattggta aggcttcgga tattcgggca gttcgcatat
     1081 aagaaagcca tcgatacacg ctcaggcata ggcgcgcttg gtcttactga ccagaaacaa
     1141 aaggaagcat atcttgcttt tcaggcgggg ggagccatgc gggatgccgc aaaacaggaa
     1201 ggcggggcag caggcgccgg ggcgggggca ggcgtaggac ttggagcggg catgggaatg
     1261 gggatgataa tggcttccca gctgggacaa acaatgcagc cccaacagac tcgtcccaat
     1321 gggataaaat gcccatcatg cagcgcagac ttgccttccg gctctaaatt ctgcactaat
     1381 tgcggcaaca agataggtcc gggaaatatg tgcccgaaat gtaacattga acttacaccg
     1441 ggcgccaagt tctgtccagg gtgcggcgca aagatcgaat aaggggggtg cggtatggaa
     1501 ataaaatgtc cacagtgcgg cggtgatgtc aaagtcatag aaggggagac atttttaaca
     1561 tgtgaatatt gttcatcagc gatctatatt gataaaagca aagtggtatt ccattatttg
     1621 ataaagccca catttgacga ggcaggagcc ttagcatctc ttcggcgctg gatggcagga
     1681 agttccactg tgaagggact ggataaggaa gcccggataa cgaaaacaga attcgtattt
     1741 tttcccatct ggtattttaa gatcaggcag gatgaaaa
//
LOCUS       DATGBN010000337         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_492122, whole genome shotgun sequence.
ACCESSION   DATGBN010000337 DATGBN010000000
VERSION     DATGBN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492122"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..944)
                     /locus_tag="VKL21_08250"
     CDS             complement(177..944)
                     /locus_tag="VKL21_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HMB45797.1"
                     /translation="MDIESLEKEFRKKVCDEIEIIREGKDRFVVATPFTFEDGDNLNT
                     ILKKNGTGWYFTDEGHTFMHLSYDDLDFERGKRKEIIDSILKTHYLENDNGELKCPVD
                     DKYFGDAFFTFIQGLIKVTDIAFTRRERILSLFYEEFQGYLRELLGDKCVFDYKDPVK
                     DPGGKYPVDCYVKNDVPLFIFGPYNNDKCRDSTITCLTYEKWGIPFTSIAIFENQEDI
                     NKRVLARFSDVIEKQYSSLTSAKERLPDYLKKISILS"
     gene            complement(953..1417)
                     /locus_tag="VKL21_08255"
     CDS             complement(953..1417)
                     /locus_tag="VKL21_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45798.1"
                     /translation="MLSDSEIQALIDDVKLLSSKVLKEIQLKNKENRAYKEYDLEVKS
                     KSGKIFRIRIRANTINILDFSVILIYVDENRKYHILRRYNGKHSHKNQIEKDKFRDFH
                     IHKATKRYSDAGFRIDGYAQVTDSYNNWKDALTKMLKDCNFKGDMSLFNGFN"
     gene            complement(1633..2085)
                     /locus_tag="VKL21_08260"
     CDS             complement(1633..2085)
                     /locus_tag="VKL21_08260"
                     /inference="COORDINATES: protein motif:HMM:NF021634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB45799.1"
                     /translation="MEKREEKIKIEFVIDTNILISALIPKNSNLRNVLLSGDFHIYAP
                     EYLLRELDKYWNLILEKAEKRGVLKSNIELTKEELLSKIIFEPDKFYNLRIKEAYAIC
                     HEFDEKDTPFVALSLMLEIPILTNDKGIIENAGVFKVLTIEEIFKKYK"
     gene            complement(2043..2261)
                     /locus_tag="VKL21_08265"
     CDS             complement(2043..2261)
                     /locus_tag="VKL21_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45800.1"
                     /translation="MKQILINVPEWVDEELVKLQMERILAIESRKRELIEETVKKLNI
                     GEEDLEQLEKIRGEVWKKEKRKLRLSSS"
     gene            complement(2518..3105)
                     /locus_tag="VKL21_08270"
     CDS             complement(2518..3105)
                     /locus_tag="VKL21_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcinarray family MAST domain-containing
                     protein"
                     /protein_id="HMB45801.1"
                     /translation="MNFKKLFFILIVLLFSFQVAAAAENEYGIVRAWFNGENATVNGI
                     QLKIGEPVEVKVEVISRIDGNSYLELNEPGVTKGFEVLEGPSKLEEMISNPKITNGWS
                     NTYFWVLKPNGNWKNGNAPINVFVSFSKKGNQKPIQFTIANPYILDEQYSGAVPTPET
                     TASPAGTQAKETPFLSVIFMVGALLLVWLLGKEKS"
     gene            complement(3105..>3323)
                     /locus_tag="VKL21_08275"
     CDS             complement(3105..>3323)
                     /locus_tag="VKL21_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45802.1"
                     /translation="HPDPIFGHEAQVYVREKLPVEDPLNDYVQIGSNTPIKFNFTTGT
                     FILVPPGKLSGIGDKDWIIEETQGFKVK"
BASE COUNT          930 a          694 c          519 g         1180 t
ORIGIN      
        1 tggattgtga agtctggggt ttgggggagt tctgatgaca ttttgatgtt ctcgaaaaaa
       61 ttaaaaaccg ccattgtacg cggatacccg ttcaacaaat ctgcgttcat ctgcgtttat
      121 ctgcggttcc atatatttaa taatcgaatt gttcggaatt tttaatatga cagttttcat
      181 gataatatcg agattttttt gagataatcg ggcagtcgct ctttggcaga agtcaaactt
      241 gaatattgct tctcaatcac atcgctgaat ctggcaagta cccttttgtt aatatcctcc
      301 tgattttcaa atatcgctat tgaagtgaaa ggtatacccc acttctcata ggttaggcat
      361 gtgattgttg aatcacggca tttatcgttg ttgtacggtc caaaaataaa taacggtaca
      421 tcatttttaa cgtagcagtc cacaggatat tttcctccgg gatcctttac cgggtctttg
      481 taatcaaaaa cacacttatc cccaagtagt tctcttaaat aaccctggaa ttcctcatag
      541 aataaagaca gaatacgctc tcttctggta aaagcaatat ctgttacttt gatcaacccc
      601 tggataaatg tgaaaaatgc atctccgaag tatttatcat ccacaggaca tttgagttct
      661 ccgttatcat tttccagata atgggtctta aggatcgaat cgattatctc ttttcgtttt
      721 cctctttcaa aatccagatc atcataggac aggtgcatga aagtgtgtcc ttcatcagta
      781 aaataccatc ctgtcccatt ctttttgagg atcgtattta aattatctcc atcttcaaaa
      841 gtaaaaggtg tggcgactac gaacctgtct ttaccttctc tgattatttc aatttcatcg
      901 caaacctttt ttctgaattc tttttcaagt gattcaatat ccatgttatc acctaattaa
      961 aaccattgaa cagagacata tctcctttga aattgcaatc cttcaacatt ttcgtgagag
     1021 catctttcca attattgtat gaatctgtta cttgcgcata accatctatt ctaaaacctg
     1081 catcggaata tctcttggtt gctttatgta tatggaaatc ccgaaattta tccttttcaa
     1141 tttgattctt atgactgtgc tttccattat atcttctaag tatgtgatat tttctgtttt
     1201 catccacata tatcagtatc acggagaaat ccagaatatt aatagtgttt gctcttattc
     1261 gaatcctgaa aattttaccc gattttgatt tgacttcaag atcgtattct ttgtatgccc
     1321 tattttcctt gtttttaagt tgaatttcct tcaggacttt ggaagataac agcttaacat
     1381 catcaatcaa agcttgaatt tcagaatcag aaagcataag ccaccacaat taaattaatt
     1441 gtattatgtc aaatggcata tatataatat tctaaaaaaa tcattaatca tacctctgca
     1501 ccacccgtgc cgcctcctcc acgcacccat caagcccacc gcgttcgatg cagggtgtaa
     1561 gtttcagcag gaattcctgg tttttgctat tttcagacat tgtaaaattg ttccttttat
     1621 ttcagatttt ttctatttat atttcttaaa aatctcttca atagttaaca ctttgaaaac
     1681 tccagcgttt tcgataatac ctttatcatt tgtcaatatt ggtatttcca acatcaaaga
     1741 taatgccacg aatggagtat ctttctcatc gaattcgtgg cagatagcat aagcttcctt
     1801 gattctcaaa ttataaaatt tatcgggttc aaaaatgatt tttgaaagaa gttcctcttt
     1861 ggtaagttct atattcgact ttagaactcc tcttttttca gccttttcaa gaattaaatt
     1921 ccaatattta tcaagctctc gtaagagata ttccggcgca tatatatgaa aatccccaga
     1981 taatagaacg ttcctgaggt tagaattttt tgggattagg gcggaaatga gaatatttgt
     2041 atctatgacg aactcaatct taattttctc ttctcttttt tccatacctc tcctcttatt
     2101 ttctcaagtt gctccaagtc ctcttcccca atattcaatt tcttcactgt ctcctcgata
     2161 agttctcttt ttctactttc gatagccaat attctctcca tttgcagttt tacaagttct
     2221 tcatctaccc attccggaac attaattaaa atttgtttca tgatcttacc tctttgtaaa
     2281 tttcggtatc gaggggcata aatattcaca ttcaaattaa tagtattatg ccaattaaca
     2341 tatatataat attgcagaat attacagcgc cagccgcgcc tcctcctcca ctcacccatc
     2401 aagctcaccg ccctcgatgc agtgagcgag tttcagcaga aatttctggg ttttgaagga
     2461 tttcctttaa tgattgggtg tgagcctccg ccttatccga cccatccgtg tgctatctca
     2521 cgatttttct tttcccaaga gccatacaag caataatgca ccgaccatga atattaccga
     2581 aaggaacggc gtctcctttg cctgagttcc cgccggggag gcggtcgttt caggcgtggg
     2641 gacggcgccg gaatattgtt cgtcgaggat gtagggattg gcgatggtga attggatggg
     2701 tttttggttt cctttcttac taaaagaaac aaaaacatta ataggtgcat tgccattttt
     2761 ccagttacca tttggcttta atacccaaaa ataagtattt gaccatccgt ttgttatttt
     2821 tggattagat atcatttctt caagtttgct aggtccttcc aagacttcaa aaccttttgt
     2881 tactccgggt tcattcaatt ccaaatacga atttccatca attcttgata ttacctcaac
     2941 tttgacttca actggttccc caatttttaa ttgtatacca tttacagtag cgttttcccc
     3001 attgaaccaa gctctaacta ttccatattc attttcggca gcagctgcta cctgaaaact
     3061 gaataacaat acaattagga tgaaaaataa ttttttaaag ttcattattt taccttgaat
     3121 ccctgcgttt cttcaattat ccaatcttta tctcctattc ctgataattt tccaggagga
     3181 acaagaataa atgttccagt tgtaaaatta aatttaattg gtgtatttga accaatttgt
     3241 acataatcgt taagagggtc ttctaccggt aatttttccc tcacatacac ctgcgcctca
     3301 tgcccgaaaa tcggatccgg atg
//
LOCUS       DATGBN010000338         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_453719, whole genome shotgun sequence.
ACCESSION   DATGBN010000338 DATGBN010000000
VERSION     DATGBN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453719"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..694
                     /locus_tag="VKL21_08280"
     CDS             152..694
                     /locus_tag="VKL21_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45803.1"
                     /translation="MTIQIDDQGWGTPVGGVGIIIVRKETFDIHYDIIPIEYFNLEMF
                     RKKAYLTGAKNIVEEGINKLNVHKEEEIEICSGCIHDKTAQWLKNEGYRFTVMKIDGI
                     AQQKGEEMFIEYLRRLGVPDPPAIVEETVDEYKAQFFYLMDWVREDPMGRAHLCKTGW
                     KYFKKFFRTKRNFEKKESYA"
     gene            687..1208
                     /locus_tag="VKL21_08285"
     CDS             687..1208
                     /locus_tag="VKL21_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HMB45804.1"
                     /translation="MPEKVIHVASLNENKVKAVREVFLSHKVKGVACNSGVREQPMDL
                     DEIIQGAIIRARSVYNECEYSIGIEDGISRVHGTISGYMNFCCCAIFDGRQIFIGLGP
                     AFEYPPECIKRVLDEGMTISQAFIPLTDRPDIGYEEGIIGWLTNGQISRADYTKHAVE
                     MAKLQIDNKRLYS"
     gene            complement(1211..>4197)
                     /locus_tag="VKL21_08290"
     CDS             complement(1211..>4197)
                     /locus_tag="VKL21_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HMB45805.1"
                     /translation="VLIKKGYTGVARAYIIYREKRSHIRKAKEFFGVKDELKLSVNAV
                     RVLERRYLLKNENGEVIETPLELFGRIAKAIAFDPESEGEFLNMLINLEFLPNTPTIM
                     NAGTDIGQLSACFVIPVEDSLVSIFDAVKSMALIQQSGGGTGFSFSHLRPGGDIVKST
                     KGVASGPVSFMRVFDTTTDIIKQGGKRRGANMGILRADHPDIIEFITAKTNEGVLTNF
                     NISVAVDDKFMNAVKEDREYELINPRNREVVKKVKARDIWNLMITMAWKTGDPGIIFI
                     DEINRHNPTPHIGEMESTNPCVAGSTLIPTEKGLIRMKDLVKKYFGGGILIAVDNRVP
                     IQIKNHNGTISLMKQKQFGVSCNEISKAFCVGEKDTCKIETESGFELICTPDHKVLTN
                     GGWVKAEDLDPLIHRVLIQSGLGRFNENFKLPFDVKNQFIGGNGKTTRLSLPDKWSKE
                     LGQVMGWLIGDGWLRDADKDCRVGFTFSEADRKVMDYFKPIINGWYGFDIKEVKRENG
                     VYHLSYHSKYFVDFFKKLGVMAVNGKDKKVPESIFTAPKGAVIGFLQGLFTADGTVNF
                     VEDKSSYVRLTSISRELLKEVQILLLNYGIKSRIYDSYELEMSKEGVLIFLKDIGFLC
                     NYHNNKINKFFTKGYYSEYFEERIKSINENGKELVYDLTEPKTLSFISNGFVSLDCGE
                     QPLLPYESCNLGSINLAKIVKNSSINWEKLEKTVNIAVRFLDNVIDINKYPLSQIEEI
                     TKGNRKIGLGVMGFADMLVQLGIPYDSEEALKTAEEVMNFIHEKSHGASEKLAKVRGA
                     FPNFKGSIYNKPIRNSTVTTVAPTGTISIIAGCSSGIEPMFAVSFVRNVLEGTQLIEV
                     NPYFEDVAKERGFYSEELMMKIAKSGTLAGIEEIPEYVKRIFVTAFDIAPLWHVKMQA
                     AFQKYCDNAVSKTINFPNDVDIKEVEKAFMLAYELKCKGITVYRYGSKSTQVLYRGDA
                     NDKYVSADPEYSGGCAAQVCPV"
BASE COUNT         1157 a          952 c          808 g         1280 t
ORIGIN      
        1 ttcaaatgtc ctgttttcta ctttctttct tgtgcttcga ggcacgccga ttcatcccac
       61 gacagaggtc gcggggattc tctacccctc taccccacgt cctaaaacga cctcagatac
      121 aatcaaatat tttaaaacat atgattaggc tatgacgatc cagattgatg accagggatg
      181 gggaacacct gtcggaggtg tagggatcat cattgtcagg aaagagactt tcgatattca
      241 ttatgatatt atcccgatag agtatttcaa ccttgaaatg ttcaggaaga aagcatatct
      301 taccggggca aaaaatattg ttgaggaagg gatcaacaaa ttgaatgtcc ataaggaaga
      361 agaaattgag atatgctccg gctgcataca tgacaagacc gcacagtggc taaagaacga
      421 gggctacagg ttcacggtca tgaagatcga cgggattgca caacagaaag gtgaggagat
      481 gttcattgaa tatcttcgca ggctgggtgt cccggatccg cctgcgatcg ttgaggaaac
      541 agtggatgag tataaagcgc aattctttta cctgatggat tgggtgagag aagacccgat
      601 gggaagggcg catctttgta agacaggatg gaaatatttc aagaaattct ttagaaccaa
      661 aaggaatttt gaaaaaaagg aaagttatgc ctgaaaaagt aattcatgtc gcttctttaa
      721 atgagaataa agtaaaggct gtccgggaag tcttcctgtc acataaagta aaaggtgttg
      781 cctgtaattc aggagtcagg gagcaaccca tggatcttga tgagataatt caaggtgcga
      841 tcataagggc aaggtctgtt tataacgagt gcgaatattc gatagggatc gaggacggga
      901 tatcccgggt acatggaaca ataagcggtt atatgaactt ctgctgttgt gccatcttcg
      961 acggtaggca gatattcatc ggtcttgggc ccgcttttga atatcctccc gaatgcataa
     1021 agcgcgtcct tgatgaagga atgaccatct cacaggcatt tattccttta actgacagac
     1081 cggatatcgg gtatgaagaa gggatcatag gatggttaac gaatggacaa atatcccgcg
     1141 cggattatac aaaacatgcg gtagaaatgg caaagctcca gatagataat aaaaggttat
     1201 attcttgact ttacaccggg catacctgcg ctgcgcaacc tcctgaatat tcagggtctg
     1261 cagacacata tttatcattc gcatcccccc ggtacaaaac ctgcgtggat ttactcccat
     1321 acctgtaaac cgtaatcccc ttgcatttaa gttcataggc gagcatgaag gctttctcga
     1381 cctccttgat atccacatca ttcgggaaat ttatggtctt tgaaaccgcg ttatcacaat
     1441 atttctggaa tgcagcctgc atcttcacat gccacagggg ggctatatca aaagctgtca
     1501 caaatatccg cttcacatac tcaggtatct cttctattcc tgccagtgta ccggactttg
     1561 cgattttcat cattaattct tcgctataaa atcccctttc ttttgcaaca tcctcaaaat
     1621 agggatttac ttcgatgagt tgtgtaccct caagtacatt cctgacaaaa gaaacggcga
     1681 acataggctc tatcccgctt gaacatcctg ctataatgct tattgttccg gtgggggcga
     1741 ctgttgtaac tgtagaatta cgtatgggtt tattatatat gcttccttta aaatttggga
     1801 aagcaccccg gactttcgcc aatttttcag aggccccgtg tgatttttca tggatgaaat
     1861 tcattacttc ttctgcggtc ttaagggctt cttcagaatc ataagggata cccagttgaa
     1921 ccagcatatc ggcaaaaccc attacaccaa gcccgatctt cctgttcccc tttgtaattt
     1981 cctcaatctg ggaaagaggg tatttattga tatcaattac gttgtcaagg aacctgaccg
     2041 caatatttac ggttttttca agcttttccc agttaataga actatttttc actatttttg
     2101 ccaggtttat tgaaccgagg ttgcaggatt catatggcaa caatggttgt tcaccacaat
     2161 caagagatac aaatccattc gagatgaagc tcaatgtctt tggctcagtc aaatcataaa
     2221 caagttcctt tccattctca tttatcgatt ttatgcgctc ttcaaagtac tcggaatagt
     2281 atccttttgt aaaaaattta tttattttat tattgtgata attacataga aatccgatat
     2341 cctttaagaa gattaaaacc ccttctttac tcatttccag ttcatagcta tcatatatcc
     2401 tacttttaat gccataattc aacagaagga tttgaacctc tttcaacagt tctctggata
     2461 tcgatgtgag tctaacgtat gatgatttgt cctcaacaaa atttacagtt ccgtcagctg
     2521 taaataaacc ctgcaagaat ccaataacag ccccctttgg cgctgtaaaa atactttcgg
     2581 gaactttttt gtctttaccg ttaacggcca ttacacccaa tttcttgaaa aaatctacaa
     2641 aatatttaga atgataggac agatgataaa ctccattttc tctttttact tcctttatat
     2701 caaaaccata ccaaccatta ataatgggtt taaaatagtc cattactttc ctgtctgctt
     2761 ccgagaaagt gaatcccacc ctgcaatcct tatcagcgtc tcttagccag ccatcaccta
     2821 taagccagcc cattacctgt cccaattctt tactccattt atccggcaaa gataatctgg
     2881 ttgttttacc atttcctcct atgaattgat tcttgacgtc aaatggtaat ttaaaatttt
     2941 cattaaatct tcccaacccc gattgtatca aaaccctatg tattaaagga tcaagatcct
     3001 ctgctttcac ccatccgcca tttgtaagaa ctttgtggtc tggtgtacag atcaactcaa
     3061 atccggattc tgtctctatt ttgcaggtat ccttctctcc cacacaaaaa gcttttgaaa
     3121 tttcattgca ggagactcca aattgctttt gcttcatgag gcttatcgtt ccattatggt
     3181 tcttgatctg tatgggaact ctgttatcca cggcaataag gattccacct ccgaaatatt
     3241 ttttaaccag atccttcatt ctgatcaggc ctttttccgt agggatcaac gtgctacccg
     3301 caacacacgg gttcgtgctc tccatctccc ctatatgcgg cgtcgggttg tgcctgttga
     3361 tctcatctat gaatatgatc cctgggtccc cggttttcca ggccatcgtt atcatgaggt
     3421 tccagatatc ccgcgctttc accttcttta ctacttccct gttcctggga ttgatcaact
     3481 catactcacg gtcttctttt actgcgttca tgaacttatc atccacagca actgatatat
     3541 taaaattcgt aaggacgcct tcatttgttt ttgcagtgat aaattcaatg atatccggat
     3601 gatcagccct gagtattccc atgttggcgc cgcgcctttt tcctccctgt ttgattatgt
     3661 ccgttgtagt atcaaacacc ctcataaatg aaacagggcc tgaagccact ccttttgtgg
     3721 attttacaat atctccccct ggccgcaggt gcgagaatga aaatcctgtc ccaccccctg
     3781 attgttggat aagcgccatg cttttcacgg catcaaaaat gctcacaagt gaatcttcta
     3841 ccggaatgac aaagcatgca gatagttgtc cgatatcggt acctgcgttc ataattgtgg
     3901 gggtattggg gagaaattca aggttaatca gcatgttgag gaactcaccc tcagattcag
     3961 ggtcaaaggc tatggctttc gcaattcgtc caaatagctc aagaggtgtt tctatgacct
     4021 ccccgttctc atttttcaac aggtagcgcc tctccagcac ccgaacggcg ttaacgctta
     4081 atttcagttc atccttcacc ccaaagaatt ctttggcttt cctgatatgc gacctctttt
     4141 ccctgtaaat aatgtatgct ctggcaactc ctgtataacc ttttttgatc aggactt
//
LOCUS       DATGBN010000339         6172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_220734, whole genome shotgun sequence.
ACCESSION   DATGBN010000339 DATGBN010000000
VERSION     DATGBN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220734"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VKL21_08295"
     CDS             <1..213
                     /locus_tag="VKL21_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HMB45806.1"
                     /translation="SIMTITKDIKIEEVVTQYPETMMVFMKHGLHCVGCHVSAFESIE
                     DGAMAHGINVDALVADLNRVISSRKP"
     gene            complement(250..1563)
                     /locus_tag="VKL21_08300"
     CDS             complement(250..1563)
                     /locus_tag="VKL21_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB45807.1"
                     /translation="MTALQTIGLEYVYADGTNALSGVNFEAHKGEKVAVLGPNGCGKT
                     TLFYHFNGLIRPSKGEVRVFGKNITKIDIDDVRKRVGLVFQESDSQLFAQSVFEDIAF
                     GPRNLRLSRDEVSERVSHVLHRFDIEALANKNPANLSSGQKKRVAIAGVIAMEPDVLV
                     LDEPTSGMDAGGITDTLGILEELNNEGKTIVISTHDSDIAASWADRIYILNKGKVFKS
                     GIPRHIFSDERIISEAGVRQPVIVQTYREFRARGISKGEVPLSVLDFIDSVDSVRVMN
                     IRCAIAGCEISAGDRVYLDIKDGMLVADKGSKGVTGIAMFDGNSGEDIAVKNVVGDIA
                     QLTGKITVFRVPGIIEGGSRAADTEKIRDFFDNNKNQKVGAMGTSAKVLLNKMGLKYD
                     FEFDVIQSAMLAALRGLNITVFASGGMADRVLEKVKERQLNCQCL"
     gene            complement(1570..2397)
                     /locus_tag="VKL21_08305"
     CDS             complement(1570..2397)
                     /locus_tag="VKL21_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HMB45808.1"
                     /translation="MLNWLTTDPLNGIIITITFFISIIIGRHLRMKRAENNGKLMDPR
                     IKILLLFFLIISITLMEHWYIPMGILVLCILISLRLNIFRNFGRKLVFPVILAFFIIM
                     IQSLTYGTNTINYGIISVSSKGFESGFLIFARVVAASSLTILLLSTASENEILETMRW
                     YRVPVTMLDISSLMSRYIKAFSMEARKMKMAQISRCGLSGGSGYRKKMHDIASITGAL
                     ITRALVRAELVYRAMVSRGWKHDRVSPGNRPLNMMDLLMGFVLSTGIIILIGIDRVI"
     gene            complement(2397..3311)
                     /locus_tag="VKL21_08310"
     CDS             complement(2397..3311)
                     /locus_tag="VKL21_08310"
                     /inference="COORDINATES: protein motif:HMM:NF006184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HMB45809.1"
                     /translation="MHISDGILEPKWIFFWYAISILFIGLGLRIINKRIKEEPVYLPR
                     LSLIGAVVFVISVWHIPVPITGSSSHPVGTPMSSIIIGPSATVVISAIALFFQAFVAH
                     GGLTTIGANTFSMGIAGNFGGYFVYRLLRNVSPLWFSAGMAGLAGSIITYLTTAFQLA
                     LSLNPGNVPYYWKLYSLGFIPTQLPLAFAEFAFTAYVIKYISETRCMENISSGYPVDR
                     FSRNVLLLMFSITAIIVIGAYIGYLGGANMAGIDSTIEAMAAPAVLPAGIGARLMSYS
                     GEPMGFALVGIIGGLVTGYFWSELRRRS"
     gene            complement(3461..3838)
                     /locus_tag="VKL21_08315"
     CDS             complement(3461..3838)
                     /locus_tag="VKL21_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45810.1"
                     /translation="MLAIFGILLATIAIAATTHNHEKTKSGLNVPAENAISRTKVPYS
                     AIFGTIDRDGKEFANGSFGEISNVSFNLPVFEISQLYPKMKAGKHTISVWCNNFEEDT
                     TIYNLIITVIANQNGKITEKSTG"
     gene            complement(3934..4338)
                     /locus_tag="VKL21_08320"
     CDS             complement(3934..4338)
                     /locus_tag="VKL21_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB45811.1"
                     /translation="MRFENRLSRDIMTRDVITVPMNDTVKHIANLLSRKRISSVIVTN
                     HDGGAMGVISDTDILKVIGKGNWENITAEHIMTHNLEFVKPTSTLTEAAIIMREKHIH
                     RLLVFSEGGVGASHRPIGILSSSDIVREVARE"
     gene            complement(4494..5195)
                     /locus_tag="VKL21_08325"
     CDS             complement(4494..5195)
                     /locus_tag="VKL21_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HMB45812.1"
                     /translation="MGEIFFKRFTFNQLAQHWIMIGTFMLLVVSGMPLKFPDAWWSPV
                     IINLLGGFAMRTQIHHAAGIGMVILGIYHVVYHVFVDKESLLDRKVWPTLKDAKDFWQ
                     TIIFYLGKAEQPKYGRYSWKEKFDYWGAFWGMVMLCVTGLIMLFPFQAMEIIPYAYVH
                     LATIVHTDEALLATLFIFIVHWWNVHYSPEVFPMSKAWLTGNLSEEQMKHEHPLEYEE
                     AMRQMNEKGDSSFSR"
     gene            complement(5256..>6172)
                     /locus_tag="VKL21_08330"
     CDS             complement(5256..>6172)
                     /locus_tag="VKL21_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMB45813.1"
                     /translation="KYVLVLAILFSLFIGITTAQEDVSCYHCHTKVVTEFRNNIHYDK
                     GFNCANCHGGADVSNASVISYSVMSGDFIGRPTRNNITDVCSKCHIKETEDYKTSIHW
                     AAIQKGHPEAATCTDCHGIHEILAIKNPNSSSNHQNSPATCAKCHSNKEMMSAWYYGI
                     KADRFDTYKESFHWRALDKGYTLVATCSDCHENHRTKSHTDPSSSTYPENLPKTCGKE
                     NCHAGVNFDAKVAGGLVHDKESLHTGELVFNKTNMDPHMKDYFLGPFDLAYWIAIFFK
                     ILTTTVIGFFTGMVILDFLSRLRIHRRF"
BASE COUNT         1817 a         1373 c         1092 g         1890 t
ORIGIN      
        1 tctattatga ccataacaaa agacattaaa atagaggaag tagtaacaca gtatccggaa
       61 accatgatgg tattcatgaa acacggcctt cattgtgtgg gatgccacgt ctctgcgttt
      121 gaaagtattg aagatggtgc aatggcgcat ggaattaatg tagatgcgct ggtcgctgac
      181 ctgaatagag tcatttcatc aagaaagcct tgaaatcagg aaaatagcct tcacttttat
      241 tacctaaagc tataaacact ggcaattgag ctgtctctct ttgacttttt ctaataccct
      301 gtcagccata ccgcccgatg caaaaaccgt tatattaaga ccacgaagcg ctgcgagcat
      361 cgctgattgt atcacatcaa actcaaaatc gtatttcaat cccattttgt tcagcaacac
      421 ctttgccgat gtgcccattg ccccaacttt ttgattctta ttattatcaa aaaagtcacg
      481 gattttttcc gtatctgcag ctcttgatcc accttcaatt attcccggca ccctgaacac
      541 ggttattttg cctgtcagct gcgcgatatc ccccactaca ttctttactg caatatcttc
      601 accgctattg ccgtcaaaca tcgcgattcc tgttacacct tttgaacctt tgtctgcaac
      661 aagcatccca tccttgatat caagatatac tctgtccccg gccgatattt cacatcctgc
      721 gatagcacac ctgatattca ttaccctgac actgtcaaca gaatctataa agtcaagaac
      781 cgaaagtgga acctcacctt ttgaaattcc tcttgccctg aattcacgat acgtctgcac
      841 gatcacgggc tgccttaccc cggcttccga aataattctt tcgtctgaaa aaatatgtct
      901 tggaataccg gatttgaata cttttccttt attaagtata taaattcggt cagcccagga
      961 ggctgcaatg tcggaatcat gggtggaaat gacgatggtt tttccttcat tgttcagctc
     1021 ttcaagaatc cccagcgtat cggtgatccc tcctgcatcc ataccggatg ttggttcatc
     1081 aagaacaaga acatccggct ccatcgcgat aactccggcg atagctactc ttttcttctg
     1141 gcctgaactc agatttgccg gattcttgtt tgcaagagct tcgatatcga accggtgcag
     1201 tacatgagaa acacgttcac taacctcatc ccgggataat ctcaagttac gaggaccaaa
     1261 agctatatct tcaaacacgg attgtgcaaa caactggctg tcagactcct ggaagacaag
     1321 tcccaccctt tttcttacat catcgatatc gattttagtg atattcttac cgaatacccg
     1381 cacttcacct tttgaaggcc tgataagccc gttaaaatga tagaataatg tggtttttcc
     1441 gcaaccattt gggccaagaa ctgcgacttt ctctcctttg tgtgcctcaa aattcacgcc
     1501 tgatagggca tttgtcccat ccgcataaac atattcaagt ccgatcgtct gtaaagctgt
     1561 catttttcct caaatcaccc tgtctattcc aataagtatg atgatgcctg ttgaaagtac
     1621 aaatcccata agtaaatcca tcatgttcaa tggccgattc cctggagaaa cccgatcatg
     1681 cttccagcct cgtgatacca tggccctata gaccagttct gcccgaacca gcgccctggt
     1741 aataagcgct ccagttattg aggcgatatc atgcattttc ttcctgtatc ctgatcctcc
     1801 ggataaaccg cagcgggaaa tttgtgccat tttcattttt cttgcttcca ttgaaaacgc
     1861 cttaatatac ctgctcatta atgaagaaat atcaagcatg gtaaccggta ccctgtacca
     1921 tcgcattgtt tctaagatct cattttcaga agctgttgac agcaagagaa ttgtcaggga
     1981 agaggctgcg accacccttg caaatatcag gaatccggat tcaaatcctt ttgaggatac
     2041 tgaaataatt ccataattaa tcgtatttgt tccataagtc aaactctgta tcataataat
     2101 aaagaaagcc aggataaccg ggaaaaccaa tttccttcca aaatttcgaa atatgttcaa
     2161 tctcaatgaa ataaggatgc aaaggaccaa aatccccatg gggatatacc agtgttccat
     2221 cagtgtaatg gaaattatta gaaagaataa taagaggatt tttattcttg gatccatcaa
     2281 ttttccatta ttttcagctc ttttcatccg cagatgcctg cctattatta tcgaaatgaa
     2341 aaaagtgata gttattatga ttccgttcaa aggatcagta gtaagccaat tcagcatcag
     2401 gatcttctcc ttaattcact ccagaaatat cctgtaacta gaccgcctat tatccccaca
     2461 agggcaaaac ccattggttc tccggagtaa gacatcaatc ttgcgccaat tcctgctggc
     2521 aacaccgctg gtgctgccat tgcttcaatt gtgctatcaa ttccagccat atttgcaccg
     2581 cccagatacc cgatatatgc gcctatcaca ataattgctg ttattgaaaa catgagcaaa
     2641 aggacgtttc tggaaaatcg gtctacagga taacctgaag agatattttc catgcatctt
     2701 gtttctgata tatattttat aacataagcg gtgaaagcga attcagcaaa tgccagcggt
     2761 agttgggtag gaataaatcc aagtgaatat aatttccaat aatacgggac atttccggga
     2821 ttaagggaaa gggcaagctg aaaagccgtt gtcaggtatg taatgatact tccggcaagg
     2881 cccgccattc ctgcagagaa ccatagcgga gatacatttc ttaggagcct gtaaacaaaa
     2941 tatcctccga aattccctgc aatccccatt gaaaaagtat ttgctccgat agtagttaac
     3001 ccgccatgag ctacaaatgc ctggaaaaat agcgcgatag cgcttataac cactgttgca
     3061 gacggcccaa tgattataga agacatgggc gttcctaccg gatgggatga cgaacccgta
     3121 atgggaacag ggatgtgcca tacagaaatt acaaaaacaa cagcgcctat cagggataac
     3181 ctgggaagat atactggttc ttctttgatg cgtttattaa ttatccgaag gcccagaccg
     3241 ataaataaga tagatattgc ataccagaag aaaatccatt ttggttccag aatcccatct
     3301 gagatatgca tatcggctcc taatcaatta aatttgtttt cactaaagtt tacttgatat
     3361 aaatagttag cgattttatc aagctgctat tagttcaaaa aaaataaaaa gccaaccgat
     3421 ggttttccta tacccccggt cttttggaag ttatgaattt ttatccggta cttttttcag
     3481 ttattttgcc attttggttt gctatgactg ttattattaa attatatatt gtggtatcct
     3541 cttcaaaatt gttacaccat acagatatag tatgttttcc ggctttcatt ttcggatata
     3601 actgtgaaat ttcgaatact ggcaaattaa aagaaacatt cgatatctct ccaaaagaac
     3661 cgttggcaaa ctcttttcca tctctatcta tcgtcccaaa tatggcacta tatggtactt
     3721 ttgttcttga tattgcattt tcagcaggta catttaggcc tgattttgtc ttttcatgat
     3781 tatgtgtcgt tgcagcaatt gctattgtgg ctaataatat gccaaatatt gccagcacta
     3841 aacctatttt aactaatctc ttttgttggt gccttcacga caaaaaacaa catctagacc
     3901 ataaatacat caggaactaa tatatttaat gtatcattcc cttgcaactt ctcttactat
     3961 atcgctcgag cttaatattc cgataggcct gtgcgatgca cctactcctc cctctgaaaa
     4021 aacaagtaac ctgtgaatat gtttttctct catgataatt gcagcttcgg tcaatgtaga
     4081 tgtgggtttt acaaattcaa gattgtgggt catgatatgt tccgccgtga tgttctccca
     4141 gttaccttta cctatgactt tcagaatatc cgtatccgat atgaccccca ttgctccgcc
     4201 gtcatggttt gttacaatga cggaggagat cctttttctg ctaagcaagt tcgcaatatg
     4261 ctttacggta tcattcatcg ggactgtaat gacatcccgg gtcattatat ctcttgataa
     4321 tcgattttca aacctcatat tattcactct cggtatttaa cctaatcctt cttttgataa
     4381 ataattaatt gatatgatta ataataaata atgattaata acagatatta tttaatccga
     4441 ttaagttaat ccaatgtgga aaaaaaaatc cggaatgaca aatagcaatg aaattatctg
     4501 gaaaaagaac tgtcgccttt ttcattcatt tgtctcattg cctcttcgta ctccagagga
     4561 tgttcatgtt tcatctgttc ctctgaaagg ttgccagtca gccatgcttt tgacatgggg
     4621 aatacttcgg ggctgtaatg aacattccac cagtgaacaa taaagataaa cagcgtagca
     4681 agtaatgcct catcggtatg cacaatagtt gccagatgaa catatgcata tggaattatt
     4741 tccatagcct ggaacgggaa taacattata agacccgtta cgcaaagcat taccattccc
     4801 cagaatgcgc cccaataatc aaatttctcc ttccagctat atctgccata ttttggttgt
     4861 tcggctttac caaggtaaaa tattattgtc tgccagaaat ccttggcatc ttttaaagta
     4921 ggccatactt ttctatcaag caatgattcc ttatcaacaa aaacatgata aacgacatga
     4981 tatatcccaa ggataaccat ccctatacct gccgcatggt ggatctgggt cctcattgca
     5041 aatcctccaa ggagatttat gattaccggc gaccaccatg catccgggaa tttcagaggc
     5101 attccactta ccactaaaag catgaaagtc ccgatcatta tccaatgctg cgcaagttga
     5161 ttgaaagtaa accttttaaa aaaaatttca cccatatttc tatctccaat ttttttagaa
     5221 tccgaaactt caattaaatt ttgccgggac atattctaga atctcctgtg gatcctgagc
     5281 cttgaaagga aatcaagaat taccatccct gtaaaaaaac ctattacggt agtggtcaat
     5341 attttgaaaa atattgctat ccagtatgca aggtcaaatg gacccaggaa ataatctttc
     5401 atatgaggat ccatatttgt tttattgaat acaagttccc ctgtgtgcaa agactcttta
     5461 tcgtggacaa gcccccctgc tacctttgcg tcaaaattca cacctgcatg gcaattctcc
     5521 tttccgcaag ttttagggag attttcaggg taagtacttg aactgggatc tgtatggctt
     5581 ttcgttctat gattctcatg gcagtcagag catgttgcaa cgagagtata gcctttatca
     5641 agtgccctcc aatggaaaga ttctttatat gtatcgaatc tgtccgcctt aatgccgtaa
     5701 taccatgcac tcatcatttc cttgtttgaa tgacacttgg cgcatgtcgc agggctgttt
     5761 tgatgatttg aagatgaatt cggatttttt atggcaagaa tttcatgtat cccatgacaa
     5821 tccgtgcatg ttgcagcttc aggatggcct ttttggatcg cagcccaatg aatgctcgtc
     5881 ttataatctt cggtctcctt tatatgacat tttgagcata cgtctgttat attatttctt
     5941 gtaggcctgc ctatgaaatc tcctgacatt acagaatatg aaattacact ggcatttgat
     6001 acatcagctc ccccatgaca attggcgcag ttaaagcctt tatcataatg aatattattc
     6061 ctgaattcag tgacaacctt ggtgtggcaa tgatagcatg aaacatcttc ttgagctgta
     6121 gtaataccaa taaataacga aaacaaaatc gctaaaacca atacatattt at
//
LOCUS       DATGBN010000340         6634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_531630, whole genome shotgun sequence.
ACCESSION   DATGBN010000340 DATGBN010000000
VERSION     DATGBN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531630"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(870..1337)
                     /gene="moaC"
                     /locus_tag="VKL21_08335"
     CDS             complement(870..1337)
                     /gene="moaC"
                     /locus_tag="VKL21_08335"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HMB45814.1"
                     /translation="MKFSHIEDDRARMVDISGKNDVLRRATATGEIQLKPETIDAIRN
                     KEIEKGSVLETARIASVLAIKNTSSVIPMCHQIPLTGIDVQFELGVENVKVIVEVKSV
                     GKTGVEMEALHGVSVALLAIWDMVKSVEKDGTGNYPSTKIKNILVIEKIKGQR"
     gene            complement(1334..2791)
                     /locus_tag="VKL21_08340"
     CDS             complement(1334..2791)
                     /locus_tag="VKL21_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMB45815.1"
                     /translation="MQAITSSRMAAIDANCEYLGIKRLQLMENAGAAVARAVKEKIRS
                     GRVVIIAGRGNNGGDAFVAARHLRDYEVTVILLGKKEDIKTPEALHNWNALEKTSIRL
                     ITGANSTAFDLSILENARVIIDGIFGTGVRGKIQDPESTIIDLINDSGAFVLSVDVPS
                     GFDPDGGEFEKAVHAGLTLTFHKMKVGLLSENAPKYTGEVSVVDIGIPVEAEFYVGPG
                     DIQPFFKRPVISHKGDAGRVLIIGGGPYSGAPTLAALGALRAGADIVTVAAPKSVSDI
                     IASFSPNLIVKELPGDILTEDDAPILAKLIENHDVVVMGMGLGNANETLSAIKKIIPL
                     CKKAIIDADALSSRFVPLFNKNIIITPHAAEMKRVSGRDVPVEQKERMGFIKDFAQKN
                     EVTVLLKGAIDLIADGTEVRANRTGNAGMTVGGTGDVLAGLTGALFARCKSPLEAAAC
                     GAFINGAAGDLAFEEFGYGLLATDIIDYIPMVMKR"
     gene            2881..3393
                     /locus_tag="VKL21_08345"
     CDS             2881..3393
                     /locus_tag="VKL21_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HMB45816.1"
                     /translation="MIQRIQQTNMKQSHIKRVRTIADYQFGRGAGEILFCDDVVFQLS
                     TTKRIRQILHDNERIATIRAKDGMLTLSIKGARKLHDFIKYPGQRVTVNSDAAPFVTK
                     GKTAFSRHVADVDPEIRAGQEVLVVDENDRLLATGKTVLSALEMQAFKKGVAVDVRSG
                     IETKDKPDRD"
     gene            3490..4758
                     /gene="thrC"
                     /locus_tag="VKL21_08350"
     CDS             3490..4758
                     /gene="thrC"
                     /locus_tag="VKL21_08350"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMB45817.1"
                     /translation="MSHVIGLKCRECGAQYPAVIQNTCYECFGPLEVNYDWDYISNHI
                     SKEKIASGPKSIWRYADLLPLESDKRIDLGAGFNKLHHAQRLGAALGLDELYILDESV
                     NPTNSFKDRVTSVAISKAIEFDVKAVGCASTGNLAAAVGAHAAKAGLPAYIFIPSTIE
                     LGKIVQMLIYGPNVIAVEGTYDDANRLASEVADSHPDWAFVNINIRPYYTEGSKTLAY
                     ETAEQLKWQVPDHIVAPMASGALLCAISRGFKELERVGLVDNSKVKISGAQPLNCSPI
                     SSAVQKNSEVIPVRNFETIAHSLAIGNPADGYYAKRTILDSEGYAASPTDTEIIEAIH
                     LLARTEGIFTEPAGGTTVAGLKKLVESGHIQHDERVVVYVTGNGLKAQDTMLKSLQRP
                     PVIKPMIGEFERLTAPGLDILGQKADLVHS"
     gene            4770..5045
                     /locus_tag="VKL21_08355"
     CDS             4770..5045
                     /locus_tag="VKL21_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HMB45818.1"
                     /translation="MVKIRFSSALSNVTHARETTFELGDTTVKAVLEKLVQQFGPDFE
                     KRILEKGEVRRFVNLYVNGEDIRHLSGLDSPVKDADEISILPAVSGG"
     gene            5074..5439
                     /gene="hisI"
                     /locus_tag="VKL21_08360"
     CDS             5074..5439
                     /gene="hisI"
                     /locus_tag="VKL21_08360"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HMB45819.1"
                     /translation="MEIKDLKFEDGLVTAIAQDYKTGEVLMVAFMDKEALKKTIETKR
                     GYYWSRSRRKLWKKGERSGHEQIVHDILIDCDADAVLLKIEQIGGACHTGYRSCFYRT
                     IEGEVIGEKLFEPDDVYKV"
     gene            complement(5461..5592)
                     /locus_tag="VKL21_08365"
     CDS             complement(5461..5592)
                     /locus_tag="VKL21_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45820.1"
                     /translation="METLIRPDLVMKRKICENCGEGTFEFGEDMRLDKYICTDCSMP"
     gene            complement(5688..>6634)
                     /locus_tag="VKL21_08370"
     CDS             complement(5688..>6634)
                     /locus_tag="VKL21_08370"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMB45821.1"
                     /translation="TAGSEEKEILASFIKQYYQDVEPPDEIIIPVEITDESISDWLCA
                     KDSKLKMPKNNVEKGLMDLAMENAAMLLKQEMLSKSFGENDTLLGLKEALSLPVVPSL
                     IEAFDISNISGTNSTGSLVSFENGLYDKKNYRRFRIKMVEGADDYAMIREVVGRAYSR
                     RKDEGKRMPDLVLVDGGKGQLNAALAAISELGLKLNIAAIAKEFEYIFLPDKKAPVIL
                     PDGSPVLLLLQRIRDEAHRFALTYHRKLREDLLYESFLDNITGIGPRKKQALLKYFGS
                     VDELRKADIHEIEKVPGITAKDAENIQKYLKSRDFENK"
BASE COUNT         1907 a         1460 c         1372 g         1895 t
ORIGIN      
        1 aaaacgccga tggaggttag aattgagaca accaataaaa aacagtactt acgaccaaga
       61 aaactggaga aatggaatgc attttcttgg gcaatacctg gtaggacaaa cccggcagta
      121 tgttagtgga cactacagtt aatgattatg ttttgttaga ttttttgaaa aaaagtcaaa
      181 aatcactgga ttttgggatt gtgccgtttt tcgagccaaa aaataataga aagtcctcgt
      241 ttatacatcc ccctgtgcct acttgacatg attattatta tgagtataag tatatgttct
      301 ttataatata acagttaata ttaaatacta atagacacat gattaatcat gctgtaggat
      361 cgggcgccta atgcctttag taccctcagt ctccttcgac ccatcgtttt tattaggcgc
      421 ctgataacta cgctttattt taaccaaatc tctatatttt tttcatattc tttatatggt
      481 gacgcaatac gataatgata atgagtataa gtataatatc tatagaatat aacagttaat
      541 attaaatact aatagaaaca tgattaaaca tgctgtagga tcgggcgcct aatgccttta
      601 gtaccctcag tctccttcga cccatcgttt ttattaggcg cctgataact acgccatttt
      661 tccaaaaaca tttatggttt tgtgtattga tccactttag aacaattatg ataatgagca
      721 taattattca catgaaataa tataacagtt aatattaaat actatcaaat catcataaat
      781 attgctgtag aatcaggcgc ctaatgcctt tagtacccct cagtctcctt cgacccatcg
      841 tttttattag gcgcctgacc agcacctatt catctttgtc ctttgatctt ttcaattacc
      901 aggatattct tgatctttgt agatggatag ttccctgtcc cgtccttttc aacagatttt
      961 accatgtccc atattgccag aagcgctacg cttaccccat gaagagcttc catttctaca
     1021 ccggtttttc caactgattt tacttccacg atcactttca cattttccac accaagttca
     1081 aattggacat caatacctgt taatggtatc tgatgacaca tcggaattac ggaagaagtg
     1141 ttctttatag ccaggacaga ggctattcgt gctgtttcaa gcaccgaccc cttctcaatt
     1201 tccttattcc tgatagcatc aattgtttcg ggttttaatt gtatctcacc agtggctgtt
     1261 gcgcgtctta aaacatcatt ttttccactt atatccacca tcctggccct gtcgtcctcg
     1321 atatgcgaaa acttcatcgt ttcatcacca tgggaatata atcgatgata tctgtagcaa
     1381 gaagtccata tccaaattcc tcaaatgcca ggtcaccagc agcgccatta ataaatgccc
     1441 cgcaagcagc ggcttcaagc ggtgacttgc atctagcaaa aagcgcacca gtcagacccg
     1501 caaggacatc accggttcct cctacggtca tacctgcatt gccagtcctg ttggctctga
     1561 cttcagtgcc atcagctatc agatcaattg cgcctttcaa taataccgtg acttcatttt
     1621 tttgtgcaaa atctttaatg aatcccattc tttctttttg ttccactggt acatcacgac
     1681 ctgataccct tttcatctca gctgcatgag gagtgattat aatattctta ttaaaaagtg
     1741 ggacgaaccg tgatgagagg gcatctgcat caataatcgc tttcttgcat aatggaatta
     1801 tctttttaat agcagaaagt gtctcgtttg cattcccaag acccataccc atcacaacaa
     1861 catcgtgatt ttcaataagc ttcgcaagaa ttggcgcatc atcttccgta agtatatctc
     1921 cgggaagttc ttttactatg agattgggag aaaaagatgc gatgatatca gaaacacttt
     1981 ttggcgcagc aactgtaacg atatcggcgc cagcccgaag agcccccaga gcggcaagcg
     2041 tcggggctcc tgaatatggc ccgcctccaa tgatgaggac tcttcccgcg tcgcctttat
     2101 ggcttatcac cggtctttta aagaaaggct ggatatcacc cggcccaaca taaaattctg
     2161 cttccacagg tataccgata tcaaccacac tgacttcacc tgtgtattta ggggcatttt
     2221 cagaaagcaa accgactttc attttatgaa aagtaagcgt tagtccggca tgtactgctt
     2281 tctcaaattc tcccccatca gggtcaaaac cggacggtac atccactgaa agcacaaaag
     2341 cgccggagtc atttataaga tctatgatag tggattcagg atcctgaatt ttcccgcgta
     2401 ctcctgttcc aaatattccg tcaattatca ctctggcatt ttctaatatg gaaaggtcaa
     2461 aggctgttga attcgcacct gtgatcagcc ggatggaagt cttttcaaga gcattccagt
     2521 tatgcagcgc ttccggtgtt tttatatctt ccttttttcc aagaagaatc acagtgacct
     2581 cataatcacg caggtgtcgc gctgcaacga aagcatcacc gccattattg cctctgcctg
     2641 caatgatcac aaccctgcca gaacgtattt tttctttcac ggcgcgggca acggcagcgc
     2701 cggcattttc catgagctgg agacgtttta tcccgaggta ttcgcaattt gcgtcgatgg
     2761 ctgccatccg ggatgaggtg attgcttgca tatattgata atcagcacat gaacacttat
     2821 aatttattga aatgataagg gatataatga aataagatat gatctattta agagcactaa
     2881 atgattcaaa gaatccaaca aacaaatatg aagcaatctc acattaaacg cgtacggacc
     2941 atcgctgatt accagttcgg gcgtggcgcc ggcgaaatcc tgttctgcga tgatgttgta
     3001 ttccagttat caaccactaa aaggattcgc cagatactcc atgataacga gcgcatagca
     3061 acgatcaggg caaaggacgg catgctgacg cttagcatca aaggagcaag aaagcttcat
     3121 gactttataa aatatccggg acagagggtc acggtaaaca gtgatgctgc gccatttgtg
     3181 acaaaaggaa agacggcatt ttcgcgtcat gttgcggatg tagatccgga aatacgagcg
     3241 ggccaggagg ttctcgtggt ggatgaaaat gaccgcctgc ttgcaactgg aaaaacagta
     3301 ctgtccgctc ttgaaatgca ggcatttaaa aaaggtgtag cggtagatgt aagaagcgga
     3361 attgagacaa aagacaaacc ggatagagat tgaacactct ttttttgttt ctgatttatt
     3421 actgtaggtt atctattgat atcgaaaata ttatatatgg taatgttgta gtatgcacag
     3481 gtgaagttaa tgagccacgt catcggatta aaatgcaggg aatgcggagc ccagtaccct
     3541 gccgttatcc agaatacatg ttatgagtgc ttcgggcctc ttgaggtcaa ttacgattgg
     3601 gattatattt caaatcatat cagcaaagag aagatcgcat ctggtccaaa atccatctgg
     3661 agatatgctg acctcctgcc gcttgagtca gataagcgca tcgacctggg ggctggtttc
     3721 aataaacttc atcatgcaca gcgccttggc gcagcattgg ggcttgatga actctatatt
     3781 cttgatgaat ccgtgaatcc tacaaattca tttaaggaca gggtaacatc agtggcaatt
     3841 tccaaagcta tcgaatttga tgtcaaagcc gtgggatgcg caagcacagg caaccttgcg
     3901 gcagcagttg gcgctcatgc ggcgaaagcc ggacttccgg cgtatatttt cataccttcc
     3961 acgatcgagc tgggaaagat cgtgcagatg ctcatctatg gccctaatgt catcgctgtg
     4021 gaaggtacat acgatgatgc caatcgtctt gcaagcgaag ttgcagattc ccatccggat
     4081 tgggcttttg taaacatcaa tatccgccca tattatacgg aaggttcaaa gacgctggca
     4141 tacgagacgg ctgagcagct aaaatggcag gttcctgatc atatagtggc gcccatggca
     4201 agcggagcgt tgctttgcgc aatctcgcga ggattcaagg aacttgaacg cgtcggactt
     4261 gtggataact caaaagtaaa gatatcggga gcacaacctc ttaactgttc tccaatatcc
     4321 agtgcagtcc agaagaacag cgaagtcatt cctgtcagga attttgagac catagctcac
     4381 agcctggcaa taggcaatcc ggcagacggc tactatgcaa aaaggaccat cctggattcg
     4441 gagggttatg cggcatcccc aaccgatacg gaaatcatag aagcgataca ccttcttgca
     4501 agaacagaag gcatattcac agaacccgcc ggcggcacga cagtagcggg tttaaagaag
     4561 cttgttgaaa gcgggcatat ccagcatgat gaaagggtgg ttgtttatgt gaccgggaat
     4621 ggtctaaaag cgcaggacac catgcttaag tcattgcagc gcccgcctgt gataaaaccc
     4681 atgatcggcg agtttgagcg ccttactgcg cccggtcttg acattcttgg acaaaaagca
     4741 gatcttgtgc attcataaag gtgatttaaa tggtaaaaat aagattctct tcagcattaa
     4801 gcaatgtaac acacgcacgc gaaacgacat ttgaattagg ggatacgact gtaaaagcag
     4861 ttcttgagaa gcttgtccag cagttcggtc ctgattttga gaagcgcata cttgaaaagg
     4921 gcgaagtacg aagatttgtg aatttgtatg tgaacggtga ggacataaga cacttaagcg
     4981 gtcttgacag ccctgttaag gatgctgatg agatatcaat tctccctgct gtgagcggtg
     5041 gataggattt ttcgtttctt tttctgaagg ttcatggaaa tcaaggatct gaaatttgaa
     5101 gatggactgg tcacggccat agcccaggat tacaaaacag gcgaagttct catggtcgca
     5161 ttcatggaca aagaagccct gaaaaagacc atcgagacaa aaagaggtta ttactggagc
     5221 aggtcaaggc gtaagctctg gaaaaagggg gaaagatcag gacatgagca aatagttcac
     5281 gacatattga ttgactgcga tgcagatgct gtactgctta agatcgagca gataggaggg
     5341 gcctgccata ccggatacag atcgtgtttt tataggacaa tagagggtga ggtcatcggt
     5401 gagaagttat tcgagcctga tgatgtgtac aaagtatgat ttttttggga tagaaattta
     5461 tcacggcatt gaacaatccg tacagatata tttgtccaat ctcatatctt caccaaattc
     5521 aaaagtgcct tcaccacaat tctcgcatat ctttctcttc attacaaggt ctggtcgaat
     5581 taaagtctcc attcaaatca ccaatcgtta attattattc atggtattta aacattgtca
     5641 tgattattca tggacatttt tatcctcggt ttaaaaaaac acttgattca tttattttca
     5701 aaatccctcg acttcaaata cttttgaata ttttctgcat cttttgcggt tatccctggc
     5761 actttttcaa tctcatggat atctgctttc cttaattcat caaccgatcc gaaatacttc
     5821 aaaagcgcct gcttcttcct gggaccaatt ccggttatat tgtcaagaaa agattcgtaa
     5881 agtaaatctt cacgcagttt cctgtgatac gtaagtgcga acctgtgggc ttcatcacgt
     5941 atccgttgaa gcagtagaag aaccggcgaa ccatccggca ggatcactgg cgctttttta
     6001 tccggaagaa agatatattc aaattccttt gcgatggctg caatattcag tttcaatcca
     6061 agttctgaaa tagcagcaag ggccgcattt aactggcctt ttcccccatc caccaggacc
     6121 aggtcaggca tccgctttcc ctcatctttt ctgcgcgaat atgccctacc cacaacctcc
     6181 cttatcatgg cataatcatc cgcaccctca accatcttta tcctgaatcg gcggtaattc
     6241 tttttatcat ataatccatt ttcaaatgaa acaagagacc ctgttgaatt agttccactg
     6301 atattggaaa tatcaaaagc ttctattaat gaaggcacaa cgggaagtga taaagcctcc
     6361 ttaaggccca gaagcgtatc attttctcca aaactttttg aaagcatttc ctgtttaagg
     6421 agcattgcag cattttccat ggcaagatcc atcaatccct tctcaacatt gttcttaggc
     6481 atcttgagct ttgaatcttt tgcgcaaagc caatcggata ttgactcatc tgtaatttca
     6541 acagggatta tgatttcatc aggaggttct acatcctggt agtattgttt tataaagctt
     6601 gcaaggatct ctttctcttc ggatccggca gtat
//
LOCUS       DATGBN010000341         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_492599, whole genome shotgun sequence.
ACCESSION   DATGBN010000341 DATGBN010000000
VERSION     DATGBN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492599"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VKL21_08375"
     CDS             complement(<1..183)
                     /locus_tag="VKL21_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB45822.1"
                     /translation="MPQKEETDNYSIKVRNLTKKFGDFTAVDNISFSVKRGETFAFLG
                     PNGAGKTTTIKMLTTLL"
     gene            296..1273
                     /locus_tag="VKL21_08380"
     CDS             296..1273
                     /locus_tag="VKL21_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMB45823.1"
                     /translation="MAKMWTSNEGFDIETARDYASKLVLLASAHRAGIFEALSEEKDI
                     EALKQALNADERALFIVLEALCALGYAHKRLDRYIISDQARSLFLERGKDYVGGSLPH
                     FLDIMEAWLKLPGIIKGAKPDRSERDVGAFMNAMASRPDKVVEETVELCLKRKSNAKN
                     VLDLGGGPGKYSKAFVNRGVKAVLYDLPDTIDYVKTHFNLGDVRNLTLKKGDFTNEFV
                     KEFSGESFDIVFMGNVCHIYSEKGNRKIIKQASSIMRSTGMIAIEDMVRGRSPMAEMF
                     AVNMLANTEDGSTYTEAQYREWLTDAGFRKIEIIDLVQKSNQLITAFLG"
     gene            1277..1735
                     /locus_tag="VKL21_08385"
     CDS             1277..1735
                     /locus_tag="VKL21_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HMB45824.1"
                     /translation="MDETDKKIIELLQQDGRMEDMEIARKIGVSHDTVKRRRNKLEDA
                     GYIKIQANINPRKLGYTNVFILGITLAPGIDVRKIAEKLVSRKEFFFVALSLGPTHSI
                     VASCFAMDQIILNKLVEELRIWKEIEKIETNIIYEVLKSGYHDISVINSE"
     gene            1750..2748
                     /locus_tag="VKL21_08390"
     CDS             1750..2748
                     /locus_tag="VKL21_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMB45825.1"
                     /translation="MTIAIEVKELVKVYGGKVRALDGVNLKVKAGDVFALLGPNGSGK
                     TTLMRILTTQFKPTSGEASIFGFDVVKRDSEIRKIISYVPQETSVWTDISGYENLLIY
                     AKIYGLQSRDRNKVIREALEGMGLDTVADNLVKTYSGGMIRRLEMACAMLIRPKILFL
                     DEPTIGLDPTARKAVWEKLTSFKKEYDTTVFFNTHYMDEADLYSDEIAIIDRGKMVKF
                     GTADELKHSIRRDVIQFSLNDHIVDESVLKRIRDLDFVNDIIINDTELSVIVVDAETA
                     LPVVMEILRNGGISVKKISMTRPTLDDVFLKYAGTISLERGDIREIKQIRSMNVKG"
     gene            2757..3521
                     /locus_tag="VKL21_08395"
     CDS             2757..3521
                     /locus_tag="VKL21_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB45826.1"
                     /translation="MKDSIREMFAMIELELRRLSHDRTELYTRAIQPILWLAVYGTVM
                     SHVKAIPTDGIPYLDYITPGVLLQSTIFVSVFYGLTIVWERETGILKKLLVAPASRYA
                     TVIGRSIASGVRAIFQVLIIIPVALLIGVDFIFNPLYFIMAILIIFFVSGGFAAISIL
                     IASIMKTRERFMGIGQALIMPLFFVSNSLYPIKLMPPILQYFAFFNPLTYAVDAVRGL
                     MISGDISKLLPDIAAIAIFDVIMFAAASISFRKIIE"
BASE COUNT         1143 a          678 c          904 g         1089 t
ORIGIN      
        1 tagaagggtc gtaagcatct ttatggttgt agtttttccc gcgccattgg gcccaagaaa
       61 tgcgaatgtc tcaccgcgtt ttactgaaaa ggaaatatta tctactgcag taaagtcgcc
      121 gaactttttt gtgagattcc tgacttttat ggagtaatta tctgtttctt ctttttgggg
      181 catatcaatc tcatagatgt tagataagtt aatatattat atcgaaccat acaatagttt
      241 actcatatat ttaatagttt gtgcgaataa atcgcataaa gaggcaagag gctatatggc
      301 aaaaatgtgg acatcgaatg aagggtttga tattgaaact gcaagagact atgcaagcaa
      361 acttgtattg ctggctagcg cccaccgggc aggaatattt gaagcactct ccgaagaaaa
      421 ggacatagaa gccctgaagc aggcactcaa tgccgatgag agggctctct ttatcgtact
      481 tgaggcactt tgcgctttgg gatatgccca taaaaggctg gacaggtata tcatatctga
      541 ccaggcacgg tctcttttcc ttgagcgagg taaggattat gttggaggtt cccttcccca
      601 ttttctcgat ataatggaag catggcttaa gcttccaggg ataataaaag gagcaaagcc
      661 cgatagatct gaacgtgatg ttggcgcttt tatgaacgcc atggcatcaa ggccggataa
      721 agtcgtggag gaaaccgtgg agctctgcct taaaaggaaa agtaatgcga agaacgtgct
      781 tgacctggga ggcgggcctg gcaagtactc aaaagctttt gtgaatagag gcgtaaaggc
      841 ggttctttat gatttgccgg atactatcga ttacgtcaaa acccatttca atctagggga
      901 tgtcaggaac ctgaccctga agaaggggga tttcacaaac gaattcgtga aggaattctc
      961 cggtgagtca ttcgacattg tttttatggg caatgtctgc catatctatt cagagaaagg
     1021 gaataggaag ataataaagc aagccagtag tattatgaga agcacaggga tgatcgccat
     1081 cgaggatatg gtaaggggac gaagccccat ggcagaaatg tttgccgtga atatgctggc
     1141 aaacacggaa gatggaagca catatactga agcgcagtac agggaatggc tcacagatgc
     1201 cgggtttcgc aaaatcgaaa taatcgacct tgtccaaaag agtaaccagc ttatcacagc
     1261 ttttttgggg taacgtatgg atgagactga taaaaagata attgaactgt tgcagcagga
     1321 cggaagaatg gaggatatgg agatagcccg aaaaattggg gtatcacacg atacggtcaa
     1381 gcgaagaaga aataaactcg aagatgccgg ttacattaaa atccaggcta atatcaatcc
     1441 acgaaagctt ggatatacaa atgtctttat cttagggata actttagccc caggcataga
     1501 tgtcaggaaa atagccgaga aactggtttc caggaaagag ttcttttttg tggcgctttc
     1561 cctgggtcct actcattcca tagttgcttc atgctttgcc atggatcaga taattctcaa
     1621 taaactggtt gaagaattaa gaatctggaa agaaatagaa aaaattgaaa ccaatatcat
     1681 ttacgaagtt ttgaaatcag gctatcacga tatttctgtg ataaactctg aatgagacga
     1741 ccctataata tgacaattgc aattgaagtc aaagagcttg ttaaggttta tggtggcaag
     1801 gtcagggctt tagatggtgt taatctgaag gttaaagcag gggacgtttt tgctcttctt
     1861 ggccccaatg gttcgggcaa gaccacattg atgcggatct taaccaccca atttaaacct
     1921 acttccggtg aagcatctat tttcggattc gatgttgtca agagggattc tgaaatcagg
     1981 aaaattatca gctatgttcc tcaggagacg agtgtctgga ctgatatcag cggctatgag
     2041 aatcttctta tatacgccaa aatttatggg cttcaatcaa gagataggaa taaggttatc
     2101 agagaggcac tggaaggtat gggtctggat actgttgctg acaaccttgt caagacatac
     2161 tctggcggca tgatcagaag acttgagatg gcatgcgcta tgcttataag accaaagatt
     2221 ctatttttgg atgaacctac gattggtctt gatcctactg ccagaaaggc tgtctgggag
     2281 aagctgacat ctttcaagaa ggaatatgac acaacagtat tttttaacac ccattacatg
     2341 gatgaagctg acctttactc tgatgagata gctataatcg atagaggcaa aatggtcaag
     2401 ttcggtacgg ctgatgaact taaacattcc atcaggagag atgtcattca attcagttta
     2461 aatgatcata ttgttgatga aagtgtttta aagaggatca gggatttaga ttttgtcaac
     2521 gacattatta ttaatgatac ggaactcagt gtaattgtcg tggatgctga gactgcgttg
     2581 cctgttgtta tggagatcct gagaaacggg ggcatatctg taaagaaaat ctctatgacc
     2641 agaccgaccc tggatgatgt tttcttaaaa tatgccggga ctatttcttt ggagagaggc
     2701 gatattcgcg agatcaagca gatcagaagc atgaacgtga agggataggt ttgacaatga
     2761 aggattcaat aagggaaatg tttgctatga tagaacttga gctgcgaagg ctcagtcatg
     2821 atagaaccga attatatacc agggcaatac aaccgatttt atggttagct gtttatggaa
     2881 ctgtcatgag tcatgttaag gctataccta ctgatgggat tccatacctt gattatatca
     2941 cgccgggcgt gttgctgcaa tctacaatct ttgtatcggt cttttatggt ttgactattg
     3001 tgtgggaaag agagaccggt attttgaaga aacttcttgt ggctccagct tccaggtatg
     3061 ctactgttat agggaggtca atagcttcag gtgtcagggc gattttccag gtcttgataa
     3121 ttataccggt ggcgctgctt attggagttg attttatatt taacccccta tacttcatca
     3181 tggcaatttt aataatattc tttgtttcag gcgggttcgc tgccatctct atactcatag
     3241 cctcaataat gaaaaccaga gagaggttca tgggcatagg ccaggctctt atcatgccgc
     3301 ttttctttgt cagcaattca ttatatccga tcaagttgat gcctccgatc ttacaatact
     3361 tcgctttttt taatcctttg acttacgcgg tggatgctgt aagaggcttg atgatcagcg
     3421 gagacattag caaacttttg cctgatattg cggctattgc aattttcgat gtaataatgt
     3481 ttgcagcggc ttcgataagc ttcaggaaaa tcatagaata agttggccaa aaaagggagg
     3541 tatctatcca tgaaaatatt taataataaa ataatctatg aaaccttaaa ggaaatcgtt
     3601 gcccctgaac acacagcctt gttgtagatc gatcctggtt tcaggataaa ggatttattc
     3661 aaaaacgaga agaaatgatt attccagcag gtttataatg aagcttaaca agtctttcaa
     3721 acatgcgttc aatatggttc tgcacagcaa actccggagc tggctgacca ttacaggcat
     3781 tgttatcggt gttgctgccg taattgcgat agtt
//
LOCUS       DATGBN010000342         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_531719, whole genome shotgun sequence.
ACCESSION   DATGBN010000342 DATGBN010000000
VERSION     DATGBN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531719"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VKL21_08400"
     CDS             <1..198
                     /locus_tag="VKL21_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45827.1"
                     /translation="DISGFADIVFAIFISMRVRFRETSVERITKNIPITTKGTRAESS
                     LRKGGLLFVLGMTVGAEELVH"
     gene            319..1254
                     /locus_tag="VKL21_08405"
     CDS             319..1254
                     /locus_tag="VKL21_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HMB45828.1"
                     /translation="MNSKHRNIKSRKSGKRPSSNLKIPIIIGLALILVLIFFLIPHGS
                     DKWSVNDEGILSYPKDRGGVDVKVLKTESGQDYALKTISFPSKDYTVEGLLRIPVSGK
                     KVPAVVILPGATVPKEGTQTLADIFSGMGYASIGIEQRNRGGVDMRFDYDLWQKGKEP
                     VEHKMVFDALRAVDVLRQEPSIDPGKIAILGESNGGRFAIIAAAIDPGISGVIGISTS
                     GYDTESQFREISDANIIKFYRSIDPETYLKFLPPRKIVMLHSVNDTIIPIDLARNTFN
                     KAIEPKQFYGVTTGKHGYSEGMKEPLERELKLMLG"
     gene            complement(1607..2473)
                     /gene="speB"
                     /locus_tag="VKL21_08410"
     CDS             complement(1607..2473)
                     /gene="speB"
                     /locus_tag="VKL21_08410"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HMB45829.1"
                     /translation="MHNIIFADANSDYEKAQYVICGVPFDGTSSYRLGSRLAPDEMRA
                     ASYNFETYCSFFDYDLEDVDIHDAGNLEVADTIDDTLLGISKYADKFVNDGKIPVILG
                     GEHSLSYPFVKACKKKHPELGFVVLDAHMDMREKYRDEKNSHACISRHVIEELTSKYV
                     SIGIRSGAREEYDYVRENKIKMFTAEDVHSQGIEKIISEFRDYIKGPVYLSIDMDAID
                     PAYAPALGTPEPYGITPRDVRAVISCLAPQIVGFDLVEIAPAYDSGGTAVLGAKLVRD
                     FIAANAKARQTL"
     gene            complement(2474..2860)
                     /locus_tag="VKL21_08415"
     CDS             complement(2474..2860)
                     /locus_tag="VKL21_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HMB45830.1"
                     /translation="MKEQVEVRTLKEGRYILIDEEPCVIKSISHAKTGKHGSAKARID
                     AIGIFDGTKRSIVQPVTDKTYVPIVERKNGQVLAVKGDVCQIMDMADYSTLELKIPED
                     YKDKIEAGKDISYLISMGKMKIDMRI"
     gene            3169..>3385
                     /locus_tag="VKL21_08420"
     CDS             3169..>3385
                     /locus_tag="VKL21_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45831.1"
                     /translation="MSQFDSKFYDFFTHPDSPGQMAAEAKRYGYSGIVVTNSKINEGI
                     LLPDDFSIYKGIEIQAKPSRIREEIKKL"
BASE COUNT          965 a          700 c          696 g         1024 t
ORIGIN      
        1 gatatttccg gatttgccga cattgtcttt gccattttta tatccatgcg ggtaagattc
       61 agggaaacaa gcgtggagag gatcacgaaa aatattccga tcacaacaaa ggggacgcgg
      121 gcagagtcgt ccctgaggaa agggggttta ttgttcgtgt taggcatgac tgtcggcgca
      181 gaggaactgg tgcattgata aggtttacta ttttatcgat gattgttgaa tcccggaagc
      241 aatttcatca ggatttttct atattttttt ctggcgaaac cacactattt atgttcaatc
      301 aaagtgaaac gatattaaat gaattcaaaa cacaggaaca ttaaatcaag gaaatcagga
      361 aaaaggccat cctctaattt gaagattccc ataataatcg gcctggcact tattcttgtc
      421 ctgatttttt ttcttatccc tcacggaagc gataaatgga gcgtgaatga tgaaggaatt
      481 ctttcatatc cgaaagaccg tggaggggtt gatgtcaaag tcctgaaaac cgaatcgggg
      541 caggattatg ctcttaagac catttctttt ccgagcaagg attatacggt agaaggactt
      601 cttcgaattc cggtttcagg aaaaaaagta cctgctgtgg taatccttcc cggagctact
      661 gttccaaagg aaggaacaca aacgcttgct gatatttttt ccgggatggg atatgcgagc
      721 ataggtattg agcagcgaaa ccgtggtgga gtggatatga gatttgacta tgatctctgg
      781 caaaaaggaa aagaaccggt ggagcataaa atggtttttg atgccctgcg ggccgtggat
      841 gttttaaggc aggagccttc gatagacccg gggaagatag caattctggg cgagagcaac
      901 ggcgggcgtt ttgcgataat cgcagcggcg atcgatccgg gaatttccgg ggtgatcggg
      961 atcagcacga gcggttacga tactgagtcc caattccgtg aaatcagtga tgctaatatc
     1021 attaaattct atcgttccat tgatccggaa acgtatctta agttccttcc tccaagaaaa
     1081 atcgtgatgc tgcattcagt aaatgacacg atcattccaa ttgatcttgc caggaataca
     1141 ttcaataaag ctattgagcc aaaacagttt tacggtgtaa ccacaggcaa gcatggatac
     1201 agcgaaggca tgaaggagcc tcttgaaaga gaattgaaac ttatgcttgg atgagccagt
     1261 ggcaaattcg aaagaatagc gtactattaa aactttcgtt caacattagc tttatagcgc
     1321 tggggttcga ccccagaccc caacggttgc gccgccgctg gcggggttta ttgagagatc
     1381 ctttgttgca aaaaatcaag atataaatat ggttgcaaga atttacgaac agaataattt
     1441 ttcaatcaaa tcctgtcgat cctgtaaatc ctgtctaaaa tttattaatt taaaggggat
     1501 ttatgggatt taagcttttg caaggctcta ttatggattt taaagtaatc ttgtcaagtt
     1561 tgccttaggc aggtctgttc ttcttctgtt tgcattatgc ggattactac agagtctgtc
     1621 gcgcttttgc attcgcagct atgaaatcac gcacaagctt tgccccaagt actgccgttc
     1681 ccccggaatc ataagccggg gcgatctcga ccagatcaaa acccacaatc tggggcgcaa
     1741 ggcatgatat tactgccctg acatcacgcg gtgttattcc atagggttcg ggtgtgccaa
     1801 gagcaggcgc ataagccggg tcgatcgcat ccatatctat tgatagatag acaggccctt
     1861 tgatataatc acggaattca gaaataattt tttcgatacc ctgggaatga acgtcttccg
     1921 ccgtaaacat ttttattttg ttttcacgga cataatcgta ttcttcacgc gctccgctcc
     1981 ttattccgat cgaaacatat ttactggtca actcctcaat aacatgcctt gaaatgcatg
     2041 catggctgtt tttttcatcc ctgtacttct ccctcatgtc catgtgcgca tcaagaacca
     2101 caaatcccaa ttcaggatgt ttctttttac atgctttcac gaacggatat gagagagaat
     2161 gttccccgcc aagaattacg ggtatcttac cgtcgttgac aaatttatct gcatattttg
     2221 aaatcccaag aagcgtatca tctatggtat cggctacttc aagatttcct gcatcatgga
     2281 tatccacatc ctccaggtca taatcaaaaa agctgcaata tgtctcaaaa ttatacgatg
     2341 ctgccctcat ctcatcaggc gccagcctgc tcccgagcct gtaacttgaa gtgccatcaa
     2401 acggcacccc gcaaatcaca tattgggctt tttcataatc ggaatttgca tccgcaaaaa
     2461 tgatattgtg catctaaata cgcatgtcga tcttcatttt ccccatggag atcagatatg
     2521 aaatatcttt tccagcttcg atcttatctt tataatcctc tggtattttt agctcaagtg
     2581 tagaatagtc ggccatatcc attatctggc acacatcgcc ttttaccgcc agaacctgtc
     2641 cgttctttct ttctacaatg gggacataag tcttatccgt aactggctgc acgattgagc
     2701 gttttgtgcc gtcaaaaatg ccaatggcat cgatccttgc ttttgctgag ccatgttttc
     2761 ccgtttttgc atgagaaata cttttaataa cacagggttc ttcatcaatt aaaatatatc
     2821 ttccttcttt taatgtccgg acttcaactt gttccttcat tgtttaatct ccttgcctca
     2881 tgatagcctc ttatatatta agtgattaaa ataagtgtat acttattttt aatcacattt
     2941 attttaaacc attctttgct ttaccgttag tctttttggg ctttatgatg acttttggaa
     3001 gcataccgct taatttaata tacttcctta taagctttat cgatatccct tccgtttcgg
     3061 aagcagagct tctatattta taataattac tatatataac aaaaatatct aaatggataa
     3121 atagagctga caaaactata tcattcgtaa gtattataaa gccggataat gtcgcagttt
     3181 gattcaaaat tctatgactt ttttacacat cctgatagtc ccggccagat ggcagcggaa
     3241 gcaaagaggt atggctattc cggaatagtc gtgaccaact cgaaaataaa tgaaggaatt
     3301 cttcttccgg atgatttttc tatttataag ggtattgaga tacaggcaaa gccatcaagg
     3361 atccgggaag aaataaaaaa attaa
//
LOCUS       DATGBN010000343         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_143808, whole genome shotgun sequence.
ACCESSION   DATGBN010000343 DATGBN010000000
VERSION     DATGBN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143808"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VKL21_08425"
     CDS             <1..760
                     /locus_tag="VKL21_08425"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMB45832.1"
                     /translation="LRPGGNRYLLDFERAGGVPAIQERLRSRLYMDTLTVTGKNIDEN
                     LKEYIIINPRANKEIIASLDSPVHAEGGIAVLIGSLAPDGSVIKQTAVSKKMLKHSGP
                     ARVFESEEQAMAAIMAKKIKSGDCLVIRYEGPKGGPGMREMLSPTAAIAGMGLIDSVA
                     LITDGRFSGGTRGPCIGHVSPEAAQGGPIALVREGDTIEIDIPGGILDLKISKEELEK
                     RRRSWKPPAPKVTKGYLARYQRLVGPADKGALIL"
     gene            911..2032
                     /locus_tag="VKL21_08430"
     CDS             911..2032
                     /locus_tag="VKL21_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB45833.1"
                     /translation="MKIKEEVMFRKLFDFAPDAIIIVNNEGLILQANTQAENIFGYAS
                     KELIGKSVDILIPERFRKSHDEHLINFIAEPRIRLMGSELELYGLRKDGTEFPVDIAL
                     GYLETESGILVLSTVRDITAHKRMEEALRISEAKYRGIFENASEGIFQTTIDGRILAA
                     NPVCVRILGYTSADELIKNVTDVRKLYAEPGRRLHLVRLIRSEGTVSEFDAIINRKDG
                     TKIWVSINAHALVDASGKVTGLQGMVMDITDRKRAQKNFQMLIDGAPDAIIAIDKDFN
                     ILLINTHTEKLFGYTRLELIGRPYNVLVPDRFRMKHAGYCKTYYANPSTKIMALHMNS
                     AALHRDGSEFPVEINMSPVETDDGIVIVIDIRDITEKKK"
     gene            complement(2037..2645)
                     /locus_tag="VKL21_08435"
     CDS             complement(2037..2645)
                     /locus_tag="VKL21_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45834.1"
                     /translation="MPKDLTVTLNDLEYEILKKMRVVEGEDGEKLRNLFRLYVSTIPE
                     LKSSEYALKRIEKKDVIDEHLRNVWAEYELTDYPTEHWNEEKVSKLISELSDINAMVK
                     VGEKQYIPSNKFRSLFKMLLHDITTESGDIDEYSAACVATIQLLMEFSVDTLRKETIR
                     DGTIFINEGWMFVYSTAIKNAREFMKTKKLFPGAQVPVQTAP"
     gene            complement(2734..3111)
                     /locus_tag="VKL21_08440"
     CDS             complement(2734..3111)
                     /locus_tag="VKL21_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45835.1"
                     /translation="MFRRFLESYVEVCNNIITEAANSVLMFEAGEQAALMIKTLKIED
                     VNKEFEKEGITTTIEVSEKKVIFHVKGLPISGNKCRYCDILRGFFGGLSRKHIDARFY
                     CKKGEECTVKGANECIFIAELIE"
     gene            complement(3211..>3714)
                     /locus_tag="VKL21_08445"
     CDS             complement(3211..>3714)
                     /locus_tag="VKL21_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45836.1"
                     /translation="FSYMDTLEGFEELPVGKEHSEVKVPIMHVELALGIRYFIKGGEI
                     GYCGLMINGVKGSGLRGRLAAVAAKSYVGRTIYIYLSEISEGKKLITVPALFEKEPSF
                     DGKLDLSGLIIKTYYHNEFKKSAIEVCNEHVNALIGKQISDNKDKLKISLLELPKKGI
                     EILKSYR"
BASE COUNT         1074 a          791 c          769 g         1080 t
ORIGIN      
        1 tctgcggccg ggagggaata gatatctttt agattttgaa cgtgcagggg gagtaccggc
       61 aatacaggag cgccttcgct caaggcttta catggatact ctgacagtaa ccggaaaaaa
      121 tatcgatgag aacctgaaag aatacattat cataaatccg cgcgcaaata aggagatcat
      181 tgcaagcctg gattctcccg ttcatgcaga aggcgggatc gccgtattga taggaagcct
      241 tgctcccgat ggctctgtaa taaaacagac agcagtcagc aagaaaatgt taaaacattc
      301 cggccctgcc cgggtatttg agagcgaaga acaggccatg gcagcaataa tggcaaagaa
      361 gatcaaatca ggcgattgcc tggtgatcag gtatgaggga ccaaaaggcg gtcctggaat
      421 gcgtgagatg ctgtctccaa ctgctgcaat agccggtatg ggacttattg attccgtagc
      481 gctcattacg gatggccgat tctcgggcgg aacgcgggga ccatgtattg gtcatgtatc
      541 tccggaggcc gcacaaggtg gaccaatagc tctggtccgg gaaggggata caattgaaat
      601 tgatataccg gggggtatat tggaccttaa gatttcaaaa gaagagcttg agaaaaggcg
      661 aaggtcatgg aaacctcctg cgccaaaagt cacaaaaggc tatcttgctc gctaccagag
      721 acttgtagga ccggccgata agggcgcttt gatactgtag caaatctgga aattgaaaga
      781 aaatccggaa attcactgta tggatattgc ttcatgagtg cgagggagag gagacgcagg
      841 ttgagtttat aaagtccata taaacgatag atataagaaa aacctttaca atagaatctt
      901 agagtaaatt atgaagataa aagaagaagt gatgttccgg aagctctttg attttgcacc
      961 tgatgccatt atcattgtta acaatgaagg gctgatttta caggcaaaca cacaggcaga
     1021 aaatatcttt gggtatgcca gcaaagagct tattggcaag tcggttgata tcctgatacc
     1081 ggagcgtttc aggaaaagtc atgatgaaca cctgataaac tttattgcag aaccccgcat
     1141 acgcctcatg gggtctgagc ttgaactcta tggtttgagg aaggatggaa cagagtttcc
     1201 ggttgacata gctcttggtt atcttgaaac agaaagtgga attctcgttt tgagtactgt
     1261 ccgtgacata accgcgcata agcgaatgga agaagcttta cggataagcg aggcaaaata
     1321 tcgcggcatt tttgaaaatg cttctgaagg gattttccag acaacaattg atggaagaat
     1381 acttgctgca aaccctgtgt gcgttcgtat cctgggttat acttcagctg atgaactgat
     1441 taaaaacgtt accgatgtcc gtaagctcta tgcagaacca ggaaggcgtc tgcatctggt
     1501 aagacttatc cggtccgaag gcactgtttc tgaatttgat gccattatta accgaaaaga
     1561 cgggaccaaa atatgggttt caataaatgc tcatgcattg gtagatgcca gcggcaaggt
     1621 cacagggctt caaggaatgg taatggatat taccgatcgc aaacgggcgc agaagaattt
     1681 ccagatgctg atagacgggg caccggatgc catcattgca attgacaagg atttcaatat
     1741 ccttctcatt aatacccata ctgagaaatt attcgggtat acacgattgg aattaatagg
     1801 acgcccatat aatgtactcg ttcccgatcg gttcaggatg aaacatgcag gatattgtaa
     1861 aacctactat gcaaatcctt caacaaaaat aatggcgctt catatgaact cagctgcatt
     1921 gcacagggat ggaagtgaat tccctgttga gatcaatatg agcccggtgg agaccgatga
     1981 tgggatcgtt atagtaattg atatacgtga cataacggaa aagaagaaat gatcttctaa
     2041 ggcgctgttt gaacgggcac ctgggcgccc gggaacaatt ttttggtctt cataaattcc
     2101 cttgcatttt tgatcgccgt tgaatatacg aacatccacc cctcatttat aaaaatcgtt
     2161 ccatctctta tcgtttcttt gcgcagagta tcgacactga actccatcaa taactgaatg
     2221 gtggcaacgc acgcagctga atattcatct atatccccgg actcggttgt tatatcgtgg
     2281 agcagcattt taaaaaggct tctgaattta tttgatggta tatactgttt ttcaccaact
     2341 tttaccatcg cattgatgtc actgagttca gagatcagtt tactgacctt ttcttcattc
     2401 cagtgttccg tgggataatc cgtaagctca tattcagccc agacattcct gagatgttca
     2461 tcgattacat cttttttttc aatcctcttt aaagcatatt ccgaggattt taattccggt
     2521 attgttgaaa cataaagcct gaaaagattc ctgagtttct ctccatcttc accttcaact
     2581 accctcattt tcttgagtat ctcatattca aggtcattaa gagttactgt caaatctttt
     2641 ggcattagtt cctcttgctt aaaatctagc cggataaatt atgccatttg atagcactat
     2701 tcttatttaa attggaatga tttccctcag cgctcattca atcagctccg caataaaaat
     2761 gcactcattt gcgcccttaa cggtgcattc ttcacctttc ttacaataaa atcttgcatc
     2821 gatgtgtttt cttgataatc ctccgaagaa tcctctcaaa atatcgcaat acctgcactt
     2881 attacccgaa attggcaaac ctttcacatg gaatattact tttttttccg atacttcaat
     2941 agtagttgta ataccctctt tttcgaattc cttattaaca tcctctattt tcagggtttt
     3001 tatcataagt gccgcctgct cccctgcttc aaacatcaat accgaattgg ctgcctcagt
     3061 tataatatta ttgcatactt ccacatatga ttccagaaat ctcctgaaca attattcaca
     3121 ccctttattt cattattaac agtcgattac cattcatatt aaggctttct cttgccaacc
     3181 caggaataga taaaagcgac cgctcgtaca tcatctgtat gatttcaata tttcaatacc
     3241 cttttttgga agctcaagca ggcttatttt aagtttgtct ttattgtcgg atatttgttt
     3301 tcctatcaat gcattcacat gttcattgca gacttcgatc gcgcttttct taaattcgtt
     3361 gtgataatag gtcttgatga tcaggccact taagtcgagc ttcccgtcaa aggagggttc
     3421 tttttcaaat aatgcaggaa ctgtgatcag ttttttccct tcggatattt cagacaaata
     3481 aatatagatc gtccttccga catacgattt tgcagccact gcggccagcc ttcctcttaa
     3541 accgctccct ttgaccccat ttatcattaa gccgcaataa ccaatttccc caccctttat
     3601 gaaatacctg atgcccagag ccaattccac gtgcattata ggaactttga cctcggaatg
     3661 ctctttgcca actggaagtt cctcaaaacc ttctaacgta tccatatatg aaaa
//
LOCUS       DATGBN010000344         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_376842, whole genome shotgun sequence.
ACCESSION   DATGBN010000344 DATGBN010000000
VERSION     DATGBN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376842"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VKL21_08450"
     CDS             <1..476
                     /locus_tag="VKL21_08450"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMB45837.1"
                     /translation="KTSLTRTESIPKYSARPPHTPSMTLSVYFGIDSVLVRLVFVVLV
                     FFNGLGLILYIILAIIMPGDGQVNKPPKETIHENMQEIGERVKEAGQGLGQAFSRKTE
                     EKHSNRAGWLGILLIMLGIFFLLDNLHFLRWVDKDLLWPIIIIFVGAWLLIKRWS"
     gene            480..1157
                     /locus_tag="VKL21_08455"
     CDS             480..1157
                     /locus_tag="VKL21_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HMB45838.1"
                     /translation="MERRPGYVGPLLLLAIGFTLLFNNLGLFPWEIWGHLLRYWPVIL
                     ILSGIQIFARHSRSGLMYFAAVFLSILLIIGAVFLAWNGYPPSTGTELREAILDNAGY
                     NDYNFADLDNANFSNSILNGADMNFATMQNVNFSNSSLKGANMNFADLKYAKFNNAGL
                     KGVNLNFADLKYADLSNAVLDGANINFVNLEGANMTGARMEGVNHRFTRTSISTICPD
                     SKNGPCW"
     gene            complement(1196..1465)
                     /locus_tag="VKL21_08460"
     CDS             complement(1196..1465)
                     /locus_tag="VKL21_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45839.1"
                     /translation="MVEIIINRRIVTERIATKLKNIAVCAPEGSCIPIALPNGDFTFD
                     VTEDIKTYWDSSNESQQKSIIRRYVEGNWSLEDVIKEEMEKNKKF"
     gene            complement(1519..2697)
                     /locus_tag="VKL21_08465"
     CDS             complement(1519..2697)
                     /locus_tag="VKL21_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HMB45840.1"
                     /translation="MRKSFQFRIYPTKKQEVALDRTLSTCRHLYNNALAERKRNADIN
                     RLKQQFDVFSWGYPEWIDYYEQKRDLTATKTEFQKEIHSQVLQDVIKRVDRSFQNFYK
                     GAGYPRFQGRNRYNSFTYPQGGYKIKDGKLTLSKIGAIKIVIHREIEGKNSRTIKTCT
                     IKKDVNHWYVSFSCKIDIPIVPMEIKTKTGIDVGLKSLVTFSNGEQIKPPKYLRVSEK
                     RLAREQIHLSKKKKWSKNRNKQRIMVAKVHRRIRNQRKDFNQKLSTNLVNKYDHVVFE
                     KLQIINMLKNHHLAKSIVDVSWSQLINLTIYKAEYSGKVVELVNPNGTSQNCICGHHV
                     PKDLSVRVHSCPSCGLVLKRDHVSAMIIEGRSSTAGIAGIQARKSNLNRETMKREAPL
                     "
     gene            complement(2844..3014)
                     /locus_tag="VKL21_08470"
     CDS             complement(2844..3014)
                     /locus_tag="VKL21_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45841.1"
                     /translation="MIKKRAWRIACSRIPTCSRRQSARPKRSRAGIALLKSMSSITFS
                     RFSRTLGRGVKG"
     gene            complement(3011..>3979)
                     /locus_tag="VKL21_08475"
     CDS             complement(3011..>3979)
                     /locus_tag="VKL21_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HMB45842.1"
                     /translation="RVLKPLIERRMAYKKKITQCTERAEEYEKKQNLLKWLLVTSFGY
                     MGYNKARFGRIECHESITAYGREILLQTIELAEEMGFEILHGIVDSLWVKGRNPAGLC
                     SRASERIGITLEHKGVFRWIVFLPNRSNGSGALNRYYGVMRNGKLKVRGIEMRRSDTP
                     ALIIRMQEEMLTCLSHAEDARGFYRTIPETLDILREYSRRMLAGECRIEDMIITARIS
                     RSIRDYRQSNSNTAAIRQFRKEGITIQPGQSIRYVLTDHASKNYMKRVKIAELADEST
                     QYDRGKYYEFLLRASESILLPFGYTKDVLDGVMRRNMQAELGRWGV"
BASE COUNT         1000 a          884 c          762 g         1333 t
ORIGIN      
        1 cgaagacaag cctgacgagg accgaatcaa tcccgaaata ctctgcaagg cctccgcata
       61 ccccatcaat gaccttatca gtttatttcg ggattgattc ggtcctcgtc aggcttgtct
      121 tcgttgtgct tgtgtttttt aatggcctgg gtttgatact atacataatc ctggctataa
      181 taatgccagg agacgggcag gtcaacaagc cgccaaaaga aacgatccat gagaatatgc
      241 aggaaatagg tgaaagggta aaggaagctg gtcaaggatt gggacaggct ttttcaagga
      301 aaaccgaaga aaaacattca aatcgcgcag gatggctcgg aattctcctt ataatgcttg
      361 gtattttttt ccttcttgat aatcttcatt ttttaagatg ggtcgacaaa gaccttctct
      421 ggcccattat tataattttc gtgggtgcgt ggttgctgat aaagcgctgg agttaaaata
      481 tggaacgaag acccggttat gtggggccgc ttctcctcct ggcgataggt tttaccttat
      541 tgttcaataa cctgggatta tttccctggg agatatgggg ccacttattg cgctactggc
      601 ctgtaatact catattgtcc ggtatacaga tatttgccag gcattcaaga tccggtctga
      661 tgtattttgc ggcagtattt ctcagtattt tattgatcat cggagcagtt tttttagcat
      721 ggaacggata tcccccatca acaggaacgg aactgagaga agctatcctg gacaatgcag
      781 gttacaatga ttataatttt gcggatcttg ataatgcaaa ctttagtaat tctattttga
      841 atggcgctga tatgaatttt gcaaccatgc aaaatgtaaa ctttagtaat tcaagtctta
      901 aaggtgcaaa tatgaatttt gcagacctga aatatgcaaa atttaataat gcaggtctaa
      961 aaggagtaaa tcttaatttt gcagatctga aatatgcaga cttgagcaat gcggttctgg
     1021 atggcgccaa tataaatttc gtgaatcttg aaggcgccaa catgacaggc gcccggatgg
     1081 agggtgtaaa tcatagattt acaagaactt caatatccac gatatgtcct gattcgaaaa
     1141 atgggccctg ctggtaatat gtatgacctt tcaggggttt tctttatttc cgttttcaaa
     1201 attttttatt tttctccatt tcttctttaa ttacatcttc taaactccaa tttccctcta
     1261 catatcttct aataattgat ttttgttgtg actcatttga ggaatcccaa taagtcttaa
     1321 tatcttcggt aacatcaaaa gtaaaatcac cattgggaag tgctataggt atgcagctcc
     1381 cttctggtgc acaaactgct atatttttca attttgtagc tattctttct gttactattc
     1441 ttctgttaat tatgatttca accataacat taacttatca catacgttga aatataattt
     1501 tgtgaactac tccccttttc aaaggggagc ttcccgcttc atcgtttctc tattgagatt
     1561 actcttacgg gcttgaattc cggcaatacc tgccgtacta cttctacctt ctattatcat
     1621 cgcagataca tggtctctct ttaacacaag accacatgaa ggacatgaat gtaccctcac
     1681 tgacaggtct tttggcacgt ggtgaccaca tatacagttc tgcgatgttc cgttaggatt
     1741 aaccaattct actactttac cagagtattc tgctttgtat atagttaaat taattaattg
     1801 tgaccacgat acatctacga tagactttgc aagatggtga ttttttaaca tattgattat
     1861 ttgcaatttc tcaaacacca catggtcata tttattgaca agatttgtac ttagtttttg
     1921 attgaaatct tttctctgat ttctgattct tcgatgtacc tttgcaacca tgattctctg
     1981 cttattcctg ttctttgacc atttcttctt cttcgataga tgtatctgct ctcttgcaag
     2041 tctcttttct gatactctta ggtactttgg cggtttaatc tgttcaccat tgctgaatgt
     2101 aaccagagat tttaagccaa catcgattcc tgttttagtc ttgatttcca tgggtactat
     2161 gggtatatct attttacatg agaaggaaac ataccaatga ttgacatctt ttttaatagt
     2221 gcatgtttta attgtgcgac tatttttgcc ttcaatctct ctgtgaatga ctattttgat
     2281 agcaccgatt tttgataggg ttaatttacc atcttttatt ttatatccac cttgaggata
     2341 tgtaaaacta ttatatctgt ttcgtccttg aaatcgtgga tatccagcac ccttatagaa
     2401 attctgaaaa cttctatcta ctcttttaat tacgtcttgg aggacttgag aatgaatttc
     2461 tttttgaaat tctgtttttg tggctgttaa atcccgcttc tgctcatagt aatcaatcca
     2521 ttcaggatat ccccacgaga acacatcgaa ttgttgtttt agtctgttaa tgtctgcatt
     2581 tcgttttcgt tctgcaagag cattattata gaggtgtctg cacgtagaaa gagttctatc
     2641 aagtgccact tcctgctttt tagttggata tattctgaat tgaaatgatt ttctcattcg
     2701 acctcaacaa aactatatac tcttttgata ctatatatcg ctttgcctat ccccaaattc
     2761 gacctcaaca atcgtttttt cgggataggt attacaattc caaggggtac gattcatccc
     2821 acgattgaaa tcgcggggat tctctacccc ttgaccccac gccctaaagt ccgtgaaaat
     2881 cttgaaaatg tgatgctgct catggatttt agcaatgcta tcccggcacg cgagcgtttg
     2941 ggacgggcgc tttgccttct ggaacaggtt ggaatccgcg agcaggcaat ccgccatgcc
     3001 cttttcttga tcaaactccc cacctcccca gttctgcctg catattcctg cgcattaccc
     3061 catccagcac atccttcgta tatccaaaag gcaaaagtat gctctcggaa gcgcgaagca
     3121 ggaactcata gtacttgccc ctgtcatact gtgtgctctc atctgcaagc tctgctattt
     3181 tcactcgttt catgtagttt ttcgaggcat ggtctgtgag cacgtatctg atactctgac
     3241 cgggctggat cgttatgcct tctttccgga actggcgtat agccgccgta ttgctgttcg
     3301 actgcctgta atcccggatg ctgcgggaaa tgcgggcagt aatgatcata tcttctatcc
     3361 tgcactcacc tgcaagcatc ctccttgaat attcccgcaa tatatcgagt gtttccggta
     3421 tggtgcgata aaaacccctg gcatcctcag cgtgtgaaag acaggtaagc atctcctcct
     3481 gcatccgtat tatgagcgcg ggtgtgtcgc ttcgccgcat ctcaataccc cgcactttaa
     3541 gttttccgtt cctcatcact ccataatagc ggttaagggc tccggacccg tttgacctat
     3601 tcggcaggaa cactatccac ctgaacactc ccttatgctc aagcgtgatc cctatgcgtt
     3661 cacttgcgcg gctgcaaagc ccggccgggt tcctgccttt cacccacagg ctgtcaacta
     3721 tcccatggag tatctcaaat cccatttcct ctgcaagttc gatcgtttgc aagagtatct
     3781 ccctgccgta tgcagttatg gattcgtggc actctattct cccgaatcgc gccttgttat
     3841 accccatata gccaaagctt gttaccagca gccatttgag gaggttctgt ttcttttcat
     3901 attcttctgc ccgttcagta cattgcgtta tttttttttt gtaagccatt cgcctctcta
     3961 tgagaggttt tagcacccg
//
LOCUS       DATGBN010000345         1888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_377039, whole genome shotgun sequence.
ACCESSION   DATGBN010000345 DATGBN010000000
VERSION     DATGBN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377039"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VKL21_08480"
     CDS             complement(<1..628)
                     /locus_tag="VKL21_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HMB45843.1"
                     /translation="MKNNYDAIIIGSGLGGLLSGAILVKKGYKPLIIEKLSFPGGKFT
                     SFNYHGYEIPTGAFHALPGGRYGNVGRLIRNLDLRVEIIEATPSFIVSTKNNHYLLPF
                     TLIDCKYLLSKNSIIRILTIKEMLQLMRIIYMVLYSNSNIPEVSIKDFVKKYTNSNKI
                     LNLLNKIISFTISTDIEDASANDFVIALRKQNRNFEGVIGGGCKALITE"
     gene            complement(870..>1888)
                     /locus_tag="VKL21_08485"
     CDS             complement(870..>1888)
                     /locus_tag="VKL21_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45844.1"
                     /translation="DFRLMGTKSTMLRSKKPVISVCAVRTGSGKSPTSQKIVEIFKEK
                     GISVVVVRHPMPYGDLLKQECQRFASMEDCLSNECTIEEREEYEPYICCGTVVYSGVD
                     FNKILKAAQDEADMIIWDGGNNDLPFFKPDLHIVIADPLRPGHELTYYPGEVNVRLAD
                     VVIVNKVDSARKEDVETVIRNVRSINDHARIIKSNSVITVEDPELIKRKRVLVVEDGP
                     TLTHGGMAFGAGTVAAKKFETLEIIDPRPYAVGSIRTVFQDFPHIGAVLPAMGYNNDQ
                     MKELEDTINASDCDAVIAGTPIDLGGLLKLNKPVIRVRYTIEEVDETLEEIIDEYLER
                     RNRK"
BASE COUNT          524 a          408 c          319 g          637 t
ORIGIN      
        1 gttcggttat tagtgcctta cacccgcccc cgattacacc ctcaaaattt cgattttgtt
       61 ttcttaatgc tataacaaaa tcatttgctg aagcatcttc aatatccgta ctgatagtaa
      121 aagaaattat tttatttaac aaatttaata ttttattgct gtttgtatac tttttaacaa
      181 aatctttaat cgatacctca ggaatattag aattagaata caaaaccata tatattattc
      241 tcatcaattg taacatttct tttatcgtaa gtatacgaat aatagaattt tttgataaaa
      301 gatatttaca atcgattaaa gtgaatggca ataaataatg attatttttt gtcgagacga
      361 taaaagaagg tgttgcttcg attatttcaa ctcttaaatc aagatttctt attaatcttc
      421 ccacatttcc atatcgtcct ccaggtaatg catggaatgc tccggttggg atttcataac
      481 cgtgataatt gaatgatgta aattttcctc caggaaaaga taatttttca atgatcaacg
      541 gcttatagcc tttttttacc aggattgctc cggataataa tccacccaat ccggagccaa
      601 ttatgatagc atcataattg tttttcattt atgcccctga taatatgtat tgcatttacg
      661 gggcaaacat tcatgcaaag cccgcattta gtgcaagtca aagatttaat actcataata
      721 ttggaatgat ttggtttgat tgaattaaat ttacagttta atttacagta tgaacaacct
      781 atgcaaccaa attcattgat ctcataatgc attaatttct cattcatttt tcccttaatt
      841 tctccatata aatctgcgtt caacggcgtt tatttacggt ttcttctttc taaatattcg
      901 tcaataattt cttcgagtgt ttcatctacc tcctcgatcg tatatcttac ccggataacc
      961 ggtttattaa gcttcaatag cccgccaagg tctatcggcg tgcctgctat gacggcatcg
     1021 cagtccgaag cgttgatagt atcttccagt tctttcatct ggtcgttatt gtatcccatg
     1081 gcaggaagga cggcgcctat atgtgggaaa tcctgaaaaa cagtacgaat ggaacctacg
     1141 gcgtatgggc gcgggtcaat gatctccaaa gtttcgaatt tttttgctgc aactgtacca
     1201 gctccaaatg ccatcccccc gtgggtgagt gtgggaccat cctccacaac aagaactcgc
     1261 tttcgtttta ttaattccgg atcttcgacg gttatgacag aattggattt tatgatcctt
     1321 gcgtgatcat ttattgatct tacattcctg atcactgttt ccacatcttc tttccgggct
     1381 gaatccactt tattaacgat tactacatcc gcaaggcgaa cattcacttc cccgggataa
     1441 taagtgagtt catgacctgg cctgagagga tccgcaataa cgatatgaag gtcgggtttg
     1501 aaaaatggga ggtcattatt cccgccatcc catatgatca tatcagcttc atcctgggcg
     1561 gctttcagta tcttattgaa gtcaactccg ctgtaaacaa cagttccgca gcagatatag
     1621 ggctcatatt cctccctttc ctctatcgta cattcgttgc ttaaacaatc ttccatcgat
     1681 gcaaacctct ggcactcctg cttcaggagg tccccatacg gcatcgggtg gcgtacaaca
     1741 actacactaa tacctttttc tttaaatatt tctactattt tttgcgaggt ggggctcttg
     1801 cctgaaccgg ttctgaccgc acataccgat atcaccggtt tctttgatcg aagcattgta
     1861 ctttttgtcc ccatcaaccg aaaatccg
//
LOCUS       DATGBN010000346         5473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_260682, whole genome shotgun sequence.
ACCESSION   DATGBN010000346 DATGBN010000000
VERSION     DATGBN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260682"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VKL21_08490"
     CDS             complement(<1..388)
                     /locus_tag="VKL21_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HMB45845.1"
                     /translation="MSEQLNIEKSAKDYENKSPQEILELALGNFKNIAISFSGAEDVV
                     LIDMAKKIGQGFRVFSLDTGRLHPETYQFIEVVRKAYSIQIEVFFANRDLTEKLVKEK
                     GLFSFYQDGHKECCDARKVDPLKRALN"
     gene            complement(401..1255)
                     /locus_tag="VKL21_08495"
     CDS             complement(401..1255)
                     /locus_tag="VKL21_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45846.1"
                     /translation="MTLEKLDIATLKKSGYMKQRQKDLFVVRLRMPCGNLTSGQLSGI
                     AGIAQKYATGNVHITMRQGIQIPNVDIHNLDIITKELEDNGTPPGSCGPRVRNITSCP
                     GNSECNYGIIDTYEMGGLLDKEFFGEDMPVKIKFAVTGCPNACAKPQENDFGVMGVLK
                     PAINTQDCTGCGTCTFMCPEKAIILEDDKIKILWDKCNLCGACVGTCPSDLITEEWEG
                     QKIFVGGKIGRHPMLGKELTTTKSAQETVAMFRKIINWSKKNTKIGERFGDCLERVGF
                     EKFRKDIL"
     gene            1454..2440
                     /gene="ala"
                     /locus_tag="VKL21_08500"
     CDS             1454..2440
                     /gene="ala"
                     /locus_tag="VKL21_08500"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMB45847.1"
                     /translation="MQNQILWLSGKEVESLLDMKGALKVVEEAFRQHGLKKVQMPPKL
                     YLYFKNHNGDLRTMPGYLEEQDIAGVKIVNVHPDNPKKGLPTVMALVILNSTESGAPL
                     AIMDGTYLTDMRTGATGGIAAKYLARKNSRTVGFVGTGNQARSQLLAINEIIDIREIK
                     ATSSSEKQTLAFKDDMKLRIECEITPKKTIKEVCDCDILVTTTPSREPIVMNDWIPEG
                     THINAIGADAPGKEELDPLILKRAKVVVDDIQQASHSGEVNVPISKGLFSVKEIFGEL
                     GEVITGKKKARTNDSDITVFDSTGLAIQDVATANLVYQSALKAKMGIKLQEF"
     gene            2541..3356
                     /gene="artA"
                     /locus_tag="VKL21_08505"
     CDS             2541..3356
                     /gene="artA"
                     /locus_tag="VKL21_08505"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HMB45848.1"
                     /translation="MIENIIWLALGLLVIASLIPLNNKIKLPLAGISWVFFSIHWMLQ
                     LKHYYDLEDYVNVFLIIFAAVFSIYIGYILIKKDRLLFRNINGINTLNSISMMTTAAA
                     IGGISYFLFAKIPSLNLWLISTVTDQTVWLGSFLGIMITKLDWNLISVNGYRVEIILA
                     CTAIESIALFLGIIASVDASFKRLASAFMISVPVIYGLNLIRNVFVVEAYGMTWFGDP
                     ETSFYIAHTIIAKMGSLIALFVIAYAVFKILPEIIDMIDGILNLVGGIFKNDG"
     gene            3346..3990
                     /locus_tag="VKL21_08510"
     CDS             3346..3990
                     /locus_tag="VKL21_08510"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HMB45849.1"
                     /translation="MMAKGSFSWISGIFLFTIFSWIAFSNLSGPISLIFFLLFEIGVI
                     LTLFFIIFFRDPERIPSGDEDDAVSPADGKVISINLRTVCIFMNIHNVHVNRAPLAGI
                     VTHIDYKPGGYIPAFNKNSDVNERNHVVFDTASGTLELTQIAGVLTRRIVSYISEGTP
                     VKRGERIGMIRFGSRVDITIPEGYVFTVGLNDKVRAAQTIIARKIDKGQHKVNI"
     gene            4008..4694
                     /gene="pssA"
                     /locus_tag="VKL21_08515"
     CDS             4008..4694
                     /gene="pssA"
                     /locus_tag="VKL21_08515"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HMB45850.1"
                     /translation="MGQNILKLIKLADIITLINALMGFASIIMTLRGQIESALVLVLL
                     AVIADGADGAVARYSGYGVLGANLDSLSDVISFGVAPAVIAFVSLSKLGYLAGVFPGL
                     FMVCGILRLARFNISGKKEGFEGIPITAGGLMVALFLLVKDLVPYFEYSFAALLFILS
                     LLMASTINYPKIKNPIILAPMAIVLVFNIAAFLNGSFDLVKKGSLLLFILIMAFFLFP
                     VWRKIYVRNQ"
     gene            4678..5034
                     /locus_tag="VKL21_08520"
     CDS             4678..5034
                     /locus_tag="VKL21_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HMB45851.1"
                     /translation="MSEINNRDRAVFEAGIKLGALYHQFVGTPINMDMIDGLARMIEE
                     SIGVQPFVRSISVSIDAEMVKKNQNPEFGYCELEGRMLHVRLQVLYKNIIAHAEMCYD
                     EEKDYPLMSVKKIEEM"
     gene            complement(5053..5424)
                     /locus_tag="VKL21_08525"
     CDS             complement(5053..5424)
                     /locus_tag="VKL21_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45852.1"
                     /translation="MGEKKQLNVRIPLELQEKIENSGRPKIDIVTEALEFYFDSRKNK
                     STGEIPGESSANQSASNISKLDKAQSDEIQRMKTEVEFLRSKIDDLLKLLHQEQVLHI
                     QTQRMLTAPPPEEKKWWQLWR"
BASE COUNT         1514 a         1047 c         1195 g         1717 t
ORIGIN      
        1 tattaagggc gcgttttaac gggtcaacct ttcgagcatc gcagcattcc ttgtggccgt
       61 cctgataaaa tgaaaacaat cctttttctt tcaccaattt ttcagtcagg tcacgattgg
      121 cgaaaaacac ttctatctga atgctatagg ccttcctgac cacctcgatg aactgatatg
      181 tctcaggatg aaggcgtcct gtatcaagcg aaaataccct gaaaccctgc cctatctttt
      241 ttgccatatc gatcaatacc acatcctcag cgccgctgaa ggatatagct atatttttga
      301 aattcccaag cgccagctcg aggatttcct gcggcgactt attctcataa tcttttgctg
      361 atttttcaat gttaagttgt tctgacattt tttcacctta tcataagata tctttcctga
      421 atttctcaaa tcccacacgc tcaaggcaat ccccgaacct ctcgcctatt tttgtattct
      481 tttttgacca gttgattatt ttcctgaaca ttgccaccgt ctcctgagca gatttggtag
      541 tcgtcaattc tttccccagc atggggtgtc ttccgatctt tccgcctaca aagatctttt
      601 gtccttccca ttcctcggta ataagatcag atgggcatgt ccccacgcag gctccgcaca
      661 ggttgcactt atcccagagt atttttattt tgtcatcttc aagaatgatg gctttttcag
      721 gacacatgaa cgtgcatgtc ccgcatcctg tgcaatcctg tgtgtttatg gcgggcttta
      781 gcactcccat taccccaaaa tcattttcct ggggttttgc gcaggcgttc ggacaccctg
      841 taaccgcgaa tttgatcttt acaggcatat cctcaccaaa gaattcctta tcaagtaatc
      901 cgcccatttc ataggtgtct atgatgccgt aattgcattc tgaatttccc ggacatgagg
      961 ttatatttcg tacccttggc ccgcatgagc ccggaggcgt gccattatct tcaagttctt
     1021 ttgttatgat atcaagattg tgaatgtcca cattgggtat ctgtatcccc tggcgcatcg
     1081 taatatgcac atttccggtt gcgtatttct gtgcgatccc ggcaatcccg gatagctgcc
     1141 ccgaggttaa attgccgcag ggcatgcgta acctgaccac aaaaagatct ttttgtcgct
     1201 gcttcatgta accggatttc tttagagttg ctatgtcaag tttttctagg gtcatggtgc
     1261 tccttgatat acaggttgat atttaaacaa tactgattcg ttaacatacg gggctaaaat
     1321 atacctgatg gaagcggcaa tatttgtaaa taacaatata atatttgtaa ataacaatgg
     1381 ttaatcataa aaatctgcaa atttgaagat aaggttttaa gctttcaggt attgattaat
     1441 tatagtggaa tcaatgcaaa accaaatcct ctggcttagc gggaaagaag ttgagtcgct
     1501 tcttgacatg aagggagcgc taaaagtcgt ggaagaagca ttcagacagc atggacttaa
     1561 gaaagtccag atgcctccaa agctttatct atattttaag aatcacaatg gcgacctgcg
     1621 gacaatgccg ggatatcttg aagaacagga tattgctggc gtaaagatag tgaatgtcca
     1681 tcccgataac ccgaaaaaag gactgccaac ggtgatggcc cttgtgatcc tgaattccac
     1741 agaatcaggc gcgcctcttg ccattatgga tggaacgtac cttacggata tgagaacagg
     1801 cgcaacaggt gggattgcag caaaatatct tgccaggaag aactcaagaa cagtgggctt
     1861 tgtgggaacg gggaaccagg caagaagcca attgttggct ataaatgaaa taattgatat
     1921 ccgtgagata aaagccacga gctcatctga aaagcagacc cttgcattca aggacgatat
     1981 gaaactgaga atagaatgtg aaattacacc taaaaaaacc attaaagaag tctgcgattg
     2041 cgatatcctc gtaactacaa ctccttcaag agaaccaatt gtgatgaacg attggatccc
     2101 ggaaggcact catatcaatg cgataggcgc ggatgccccg ggaaaagaag agcttgaccc
     2161 gctgatcctg aagagagcaa aggttgttgt tgatgatatc cagcaggctt cacattcggg
     2221 agaggtcaat gtgccaatat cgaagggatt attttctgtg aaagagattt ttggggaact
     2281 tggcgaggta attacgggga agaagaaagc aaggacgaat gattcggata ttactgtatt
     2341 tgattccact ggacttgcta ttcaggatgt tgcaactgcg aatctggtgt atcagagtgc
     2401 attaaaagct aagatgggaa taaaattgca ggagttctaa tctttcggat ttcaggaagc
     2461 atttatcagt aaaaccaggg tcaatggtag atttaatatg gttgtagagg ctattctgac
     2521 aaaaaaagtg aaaaagaaac atgatagaaa atatcatttg gctggcgctt ggattgcttg
     2581 ttatagcttc gcttatacca ctgaataata aaatcaaact acctcttgca ggaataagct
     2641 gggtgttttt ttcaatacac tggatgctcc agttgaagca ttattatgac cttgaagatt
     2701 atgtcaatgt atttttaata atttttgccg cggtttttag catttacata ggatacattc
     2761 tgataaaaaa ggacaggtta ctgttcagga atatcaatgg tataaataca ttgaattcaa
     2821 tttccatgat gactacggct gccgcaatag gcggtatttc ttattttctc ttcgccaaga
     2881 tcccttcatt gaatttgtgg ttaatttcca cggtaactga ccagacagta tggctgggat
     2941 cgtttcttgg aataatgata acaaagcttg attggaacct catatcagtg aatggttaca
     3001 gggtggagat aatccttgca tgcacggcta ttgaaagtat tgcattattt ttggggataa
     3061 tagcaagtgt cgatgcatct tttaaaaggc tggcgtcggc ttttatgata tctgttccgg
     3121 taatatacgg tttgaacttg ataagaaatg ttttcgtggt tgaagcttat gggatgacat
     3181 ggtttggcga ccctgaaacg agtttttata tagctcatac tattatcgca aaaatgggtt
     3241 cattgatagc attgttcgtg atagcttacg cggtctttaa aatactaccg gaaattatcg
     3301 atatgatcga tgggatactg aatctggtgg gagggatttt taagaatgat ggctaaaggt
     3361 tcgttttcat ggatctcggg aatatttctt tttaccatct tttcatggat cgcatttagt
     3421 aacttgagcg gcccaatctc attgatcttc tttcttttat tcgaaatcgg ggtcatactc
     3481 acgttgtttt tcattatttt ttttcgcgat cccgagcgaa taccatcagg ggatgaggat
     3541 gatgcagtct cgcctgctga tggaaaagtg atatctatca acctgaggac agtatgcatt
     3601 tttatgaata tccataatgt tcatgtgaac cgagctcctc ttgcgggaat agttactcat
     3661 atcgattata aaccgggagg atatatcccg gcctttaaca agaattctga tgtaaatgaa
     3721 aggaaccatg ttgtttttga cactgcgtca ggaactcttg aattaacaca aattgcaggt
     3781 gttctgacaa gaaggatcgt ctcttatatc agcgaaggaa cgcctgttaa aaggggtgag
     3841 cgcatcggaa tgatacgttt tggctcacga gtggatataa cgataccgga aggatatgtt
     3901 tttactgtcg ggctgaatga taaggtaaga gctgcacaga ccataattgc caggaaaata
     3961 gataaaggtc aacacaaggt aaatatctga aaaataggga aatagaaatg ggacaaaata
     4021 tattaaaact tattaagctg gctgatatta ttactcttat caacgctttg atgggatttg
     4081 cttctataat aatgacactg cgcgggcaga tagaaagcgc gctggttttg gtgcttcttg
     4141 ctgtgatagc cgacggcgct gacggtgcgg tagccaggta ttccggatac ggtgttcttg
     4201 gtgcgaatct ggactcatta tccgatgtta tatcgttcgg ggtcgcacca gcagttattg
     4261 catttgtttc tctttcaaaa cttgggtatc tggcaggagt ttttcccggt cttttcatgg
     4321 tttgcggtat attacgtctt gccaggttca atatttccgg gaaaaaagaa ggatttgaag
     4381 gaattcctat aacagctgga ggtttaatgg tggctttatt tctcctggta aaagatcttg
     4441 ttccatattt tgaatattct tttgctgcgc ttctttttat attatcgttg ttgatggcaa
     4501 gcacgataaa ttatcccaaa attaaaaacc ccattatcct tgcacctatg gcaatagttc
     4561 tcgtattcaa tatcgctgct tttcttaacg gatctttcga cctggtgaaa aaaggttcat
     4621 tgttgctgtt catacttatt atggctttct ttttgttccc ggtttggagg aaaatatatg
     4681 tccgaaatca ataaccgtga cagggcagtt tttgaagcgg gaataaagct gggagcattg
     4741 tatcaccagt tcgttggcac tcccatcaat atggatatga ttgatggtct tgccaggatg
     4801 atagaggaaa gcataggagt ccagcctttc gttagaagca tcagtgtttc aatagatgcc
     4861 gaaatggtaa aaaaaaatca gaatcctgaa ttcggatatt gtgaattgga aggtcgcatg
     4921 cttcatgtaa ggttacaggt gctttataag aatattatcg ctcatgcaga aatgtgttat
     4981 gatgaagaaa aggattatcc tttgatgagt gtgaagaaga tcgaagaaat gtgatcacag
     5041 attctggttt attcatcgcc ataactgcca ccattttttc tcttctggcg gtggcgctgt
     5101 aagcattcgt tgtgtctgga tatgaaggac ctgctcctgg tggagcagct taagaaggtc
     5161 atctattttt gatctcagga attctacttc agttttcatt ctttgtattt catcgctttg
     5221 tgccttgtca agcttgctaa tattgctagc actttgattg gcgctgcttt cacctggtat
     5281 ctcacctgtg cttttgtttt tcctgctatc aaaataaaat tcaagggctt cagttacaat
     5341 atcgatttta ggcctgccgc tgttttcaat tttttcctga agttctaaag gtatcctcac
     5401 attaagctgc ttcttttctc ccatgcttat ataactgaat aactatttta tatctttttc
     5461 tatcaggaaa aga
//
LOCUS       DATGBN010000347         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_532282, whole genome shotgun sequence.
ACCESSION   DATGBN010000347 DATGBN010000000
VERSION     DATGBN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532282"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1134
                     /gene="ygiD"
                     /locus_tag="VKL21_08530"
     CDS             328..1134
                     /gene="ygiD"
                     /locus_tag="VKL21_08530"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HMB45853.1"
                     /translation="MKKANSTKMPVLFIGHGSPMNMILKNNFTDSLVRLGKELPEPGA
                     ILVISAHWLTNGSYVTCNEKPEIIYDFYGFPEELYEIEYPSPGLPRDIRYLIDSMKKL
                     DIKCSANWGLDHASWAILRHMYPAADIPVLELSLDYSFNEWNPKPIQYHFDIASELAF
                     LREKGILIIGSGNIVHNLGLIDFRNIDAAPYSWAVDFDEEVKSNLMNMNHAKLIDYQK
                     LKGSYLAVPTLDHYLPMIYALALQEEGEPLKFTYEGFQHGSISMRCFQIG"
     gene            complement(1151..>1694)
                     /locus_tag="VKL21_08535"
     CDS             complement(1151..>1694)
                     /locus_tag="VKL21_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45854.1"
                     /translation="TLNRKWLIEYIRELKRKNTDDKAHFHVDTNGSILTPDYLDELVE
                     AGMTDIGIDLKSLELNTFSHITGVNNREIAKKYLETSWNAVKYIVDNYPEKVFLGIGI
                     PYNKDLISLDEIRKMGDKIRKIDENMQVCVLDYRPVFRRSYLQRPEYEEMVNVWKNLS
                     GTGLKTVICQTVKGHIGPEL"
BASE COUNT          534 a          318 c          312 g          530 t
ORIGIN      
        1 acttaaaggc ttctatacat aacgattact tattttggag aatgaaattt taagataaaa
       61 atgcagattt ataaaaatta gaagttattc ataaaacgat attggtggaa atgattgtat
      121 tccctaaccg atgaccaatg ggtaataatc tgactcacgt ttcaaagaaa cgtgagtttg
      181 aagattccca tttttccctt ttgatctaac ctccagcgtt cgcaggcaaa agaagaagag
      241 aacacaacaa atgccatccg atgctggcaa gttggcagac aaacaaatat taatcatgaa
      301 agttacagta taaattgagg tgaaaatatg aaaaaggcta acagcactaa aatgccagta
      361 ttgttcatag gtcatggttc acccatgaac atgattttga aaaacaactt tacagatagc
      421 cttgttagat tgggaaaaga attgccggaa ccaggggcga tattggtcat ttcggcacac
      481 tggctaacta atggatcata tgttacgtgc aatgagaagc cagagataat atatgatttt
      541 tatggatttc cagaagagct atatgaaata gaatatccaa gtcctggctt accccgcgat
      601 atcaggtatt taatagactc aatgaaaaaa ttagatatca aatgcagcgc caactggggg
      661 ttagaccatg cttcgtgggc aattttaaga catatgtatc ccgcggcaga tattcctgtt
      721 cttgaactga gccttgatta ttcttttaac gagtggaatc caaaaccaat acaatatcat
      781 tttgatattg cctcagaact tgcattttta agagaaaaag gaattctcat aataggcagc
      841 ggcaatattg ttcataatct tggactcatt gattttcgaa atattgatgc agccccatat
      901 agttgggctg tcgacttcga tgaggaagta aaatcaaatt taatgaatat gaatcatgca
      961 aagctcattg attatcaaaa actaaaagga tcatatcttg cagtaccgac actcgaccat
     1021 tatttgccaa tgatatatgc gctggctttg caggaggagg gtgagccttt gaagttcaca
     1081 tatgaaggat ttcaacacgg ctcaatttcc atgaggtgtt ttcagatcgg ataaaaaagc
     1141 atcatttcgg tcataattcc ggccctatgt gacctttgac agtctggcag attacagttt
     1201 ttaatcctgt tccagaaaga tttttccaga cattgaccat ttcttcatat tctggtctct
     1261 gtaggtatga tcttcgaaat actggcctgt aatccagtac gcagacctgc atattctcat
     1321 cgatctttct tattttatcc cccatttttc ggatctcatc gagtgagatg aggtccttgt
     1381 tataaggaat tccaatacca agaaacactt tttcaggata attatccaca atatacttta
     1441 ccgcattcca tgaagtttcc aggtattttt tggctatctc cctgttattt acacctgtta
     1501 tgtgcgaaaa tgtattaagc tcaaggcttt tgaggtctat gccgatatct gtcatcccag
     1561 cttcaaccag ttcatccaga taatcaggtg tcagtatcga accatttgtg tccacatgaa
     1621 agtgtgcctt atcatcagta tttttccttt tcaattctct gatatattca attaaccatt
     1681 tcctattcag agta
//
LOCUS       DATGBN010000348         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_144204, whole genome shotgun sequence.
ACCESSION   DATGBN010000348 DATGBN010000000
VERSION     DATGBN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144204"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VKL21_08540"
     CDS             <1..689
                     /locus_tag="VKL21_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HMB45855.1"
                     /translation="TRIRTDPWKPLPIMAQTSEDILRSQGSIYVHSLTMETLMSAIKR
                     GSRSIGFVGPSCNIDAVYKMQNSPYGLLHMFMRANILKMGLFCMDTFNYEGLKTFLVS
                     KGILLSDVNEMKIRKGKFSVKTDSDHIFPLNDINAFRNGSCTVCTDLTAEKADISFGG
                     VGSKEGYTTVLARTGLGLELFKNAEDRGYIKIEPLEREGLERVLHQARAKKVQMYMIK
                     RRVEMGALPI"
     gene            1388..2014
                     /gene="wrbA"
                     /locus_tag="VKL21_08545"
     CDS             1388..2014
                     /gene="wrbA"
                     /locus_tag="VKL21_08545"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HMB45856.1"
                     /translation="MAKNKLNILIVFYSMTGNVAKLAKAVAEGAGSVKDTEVRIRQVE
                     ELIPKEKWNDIMKKVKEELKDIPIASMGDLEWANGIAFGTPTRYGNMTAQMKEYIDET
                     GGLWQKGALENKVAGIFTSTSTQHGGQETTIITSMVPLFHLGMIVVGIPYSEQRLFEF
                     DSISGGSPYGASSISGPMADRSPTSNELEIAKTLGRRIAEVSRKISGK"
     gene            complement(2069..2581)
                     /locus_tag="VKL21_08550"
     CDS             complement(2069..2581)
                     /locus_tag="VKL21_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMB45857.1"
                     /translation="MNDTNDQNNILDILKEAISIEIYGKEYYSIFSELIEEENAKSIF
                     RGLAGDEGDHRVLLEKEYRKVSGKSLEAGVMDEKNREKARQVFPESLEPMNITETQEA
                     LKIGIRTEERSIELYSNSAQETGIRSSKDLFLTLVDFEKEHKKILEDALYYLGQEGSW
                     YGYSPPTIEG"
     gene            complement(2625..2930)
                     /locus_tag="VKL21_08555"
     CDS             complement(2625..2930)
                     /locus_tag="VKL21_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45858.1"
                     /translation="MPLPGYLPSEGLPVIAAWFASNVGAYTIAGKLLADGILTSRQII
                     VILLAGRVLSSLVRLRFTIPYYTGIFSPGLGMQIMLLATLMQEGLTVIVIMLLVSFG"
     gene            complement(2861..>3477)
                     /locus_tag="VKL21_08560"
     CDS             complement(2861..>3477)
                     /locus_tag="VKL21_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HMB45859.1"
                     /translation="LMELLIDLGWIQKLGFVTAPFMHFGHLREEVGVSFLASFGSPTA
                     GNSMIADLNKKGLIDRKETLIASLVTSFPATFIFVRDLLPVLIILLGSTGVIYLGIVV
                     AVGLLRTGITLVLGRILLPPKKSPVIPQKIRKKKFRQALQSALLASWPPLKNIIPSMV
                     IAAIIVFQLIDAGFFNIISSYICHYPDISHQRAYLSLLHGLQVT"
BASE COUNT         1009 a          727 c          759 g          982 t
ORIGIN      
        1 tgacacggat acgcacggat ccgtggaaac cactgccaat aatggcgcaa accagtgaag
       61 atatcctccg ctcccagggt tccatatatg tgcattcact caccatggaa accctgatga
      121 gtgcgataaa aagaggctcg cgttccatcg gctttgttgg tccatcatgc aacatcgatg
      181 cagtttataa aatgcagaat tcgccatatg gattgctgca catgttcatg agggcaaata
      241 tcctgaaaat ggggcttttc tgtatggaca ctttcaatta tgaaggtctg aaaaccttcc
      301 tcgtttcaaa gggtatccta ttatctgatg ttaacgagat gaagatacgc aaaggaaaat
      361 tcagtgtaaa aacggattcg gatcatatct ttcctcttaa tgatatcaac gcattcagga
      421 acggttcatg cactgtctgc acagacctga cagctgagaa agcggacatc tcttttggcg
      481 gcgttggttc aaaggaagga tatacaactg ttcttgcccg caccgggctg ggacttgagc
      541 tttttaaaaa tgctgaggat agggggtaca ttaagattga gccacttgag agggaggggc
      601 ttgaaagagt tctgcaccag gcaagagcca agaaagtgca gatgtacatg ataaagagaa
      661 gggttgaaat gggcgcctta cccatttaac cctctatcgt ggcggagaat aaccgtgcca
      721 aaaaccttcc tggcccagat aatacaatgc agcttcaaga atctctttgt gacctttttc
      781 aaaattcacg agcttaaaaa aaaggtcttt gcttgatata atgtctgttt tttgagcgct
      841 atttgaatat aattcaatag acctctcttc agttctgatc ccaattttca gagcttcctg
      901 gtttcggtta ttgtcatcgg ctcaagtgat tctgggaaaa cccgcctggc ttccctgttc
      961 ttttcatcca tcaccccttc ttcaatgggt tttcctgata tctaccggta ctctttctca
     1021 aggagctgct tgtggtcccc ttcatccctg gaaagccttc tgaaaatcga tttagcattt
     1081 tcttcctcaa cgatgtcgct aaatatcgaa tagtattcct ggccatagat ctcgatcgaa
     1141 atcgcttctt tcaggatatc tataatattg ctttgattgt cagtattatt gacccattaa
     1201 aggttgattt caaatgataa taaagatagt gtaatgattg atataattga gaaatttttt
     1261 tggatatttt aaatatgttc ttgcatttat caaagtagaa atgcggttct taaagttagt
     1321 tgtatttctt cagatagtta gcaataatta aatagtttgc acactatgca atattctggg
     1381 agtttttatg gcaaaaaata aattgaacat acttatcgtc ttctacagca tgaccggaaa
     1441 tgttgcaaaa ctggcaaaag cggtagctga aggggcgggg agcgttaagg atacagaagt
     1501 gagaattaga caggttgaag agcttattcc caaagagaag tggaacgaca ttatgaagaa
     1561 agtgaaagaa gaactgaaag atattccaat tgcttcaatg ggtgatctgg agtgggctaa
     1621 tggcatagca ttcggaactc caacaagata tggcaatatg acggctcaga tgaaggaata
     1681 tatagatgag accgggggcc tctggcaaaa aggcgctctt gagaacaagg tggcaggcat
     1741 ctttacaagc acaagcaccc agcacggggg tcaggaaaca acaataatta cttcgatggt
     1801 accactattt catctgggaa tgatcgtcgt gggaattcct tattctgaac agaggttgtt
     1861 cgaatttgat tcgatatcag gcggctctcc atatggtgcc tcttcaatat ctggaccgat
     1921 ggccgataga tcccctactt ccaatgaact cgaaattgca aaaacacttg gcaggagaat
     1981 agctgaggtt agcagaaaga tttcaggtaa ataaatcatt taatcatttt atttcatgat
     2041 aaagagaagg gttgaaacgg gaatccattc aaccttctat agtgggaggg gaataaccgt
     2101 accatgagcc ttcctggccc agataataca gtgcatcttc aaggatcttt ttatgttctt
     2161 tctcaaaatc caccagagtc aagaaaaggt ctttgcttga cctgatgcct gtttcctggg
     2221 cactatttga atataattct attgacctct cttcagtcct gatcccgatt ttcagggctt
     2281 cctgtgtttc ggttatgttc atcggctcaa gtgattcagg aaaaacctgg cgggcttttt
     2341 ccctgttctt ttcatccatt acacccgctt ctaaggattt tcctgatacc ttcctgtact
     2401 ctttctcaag gagtaccctg tggtctcctt catcacctgc cagccctctg aagatcgatt
     2461 tggcattttc ctcttcaata agttcgctaa atatcgaata gtattcctta ccatatattt
     2521 cgatagagat cgcttctttc aggatatcaa gaatattgtt ttgatcgttc gtatcattca
     2581 cccatcaaag gttgatttca aactgtaata aagatagcgt atcttcaccc gaatgaaaca
     2641 agcagcatta tgactattac cgtcagtcct tcctgcatca aggtggcaag aagcatgatc
     2701 tgcataccca ggccgggtga gaatatcccg gtataataag gtatcgtgaa cctgaggcgc
     2761 acaagacttg aaagaactct tcctgctaac agaataacta tgatctgcct ggatgtcagt
     2821 attccatctg ccagcagctt ccctgcaatg gtgtatgcac ctacgttact tgcaaaccat
     2881 gcagcaatga caggtaggcc ctctgatggg agatatccgg gtaatggcaa atgtaagatg
     2941 atattatatt aaaaaaaccg gcatcgatga gctggaacac tatgatggca gcaatcacca
     3001 ttgaaggaat aatattctta aggggaggcc atgaggccaa aagagcgctc tgtaaggctt
     3061 gcctgaattt cttcttcctt atcttctgag ggattacagg ggatttcttt ggaggcagta
     3121 aaatgcgccc aaggacaagt gtgatcccgg tgcgaagaag acctacggct acaacgatac
     3181 ccagatagat cacacctgta gaaccgagca atattataag gacggggagc agatccctta
     3241 caaagatgaa tgttgcagga aaagaagtta caagggaagc tatcagtgtc tctttcctgt
     3301 caataagacc ttttttgtta aggtcagcga tcatggaatt ccctgccgtg ggtgagccaa
     3361 aggaagcaag gaagctcacc ccaacctctt ccctgagatg cccgaaatgc atgaacggcg
     3421 ctgtaacaaa accaagcttt tgtatccagc ccaggtctat caacagttcc atcagga
//
LOCUS       DATGBN010000349         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_26957, whole genome shotgun sequence.
ACCESSION   DATGBN010000349 DATGBN010000000
VERSION     DATGBN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26957"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VKL21_08565"
     CDS             complement(<1..160)
                     /locus_tag="VKL21_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45860.1"
                     /translation="MSRIRVLVVDDSAFMRKIVTDILAESPDIEVIGKAKNGQEAIEK
                     VTKLRPDVV"
     gene            complement(172..528)
                     /locus_tag="VKL21_08570"
     CDS             complement(172..528)
                     /locus_tag="VKL21_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB45861.1"
                     /translation="MAGKILLVDDAAFMRMMLKKIISPTGHELVEGVDGSDGVAKYKE
                     HKPNLVFLDIVMPNIDGIECLKQIMAFDSNAKVIMCSSIGQQTVVNDAIKIGARDFII
                     KPFDAAKVLEAVSKNM"
     gene            complement(648..2300)
                     /gene="flaJ"
                     /locus_tag="VKL21_08575"
     CDS             complement(648..2300)
                     /gene="flaJ"
                     /locus_tag="VKL21_08575"
                     /inference="COORDINATES: protein motif:HMM:NF004704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellar assembly protein FlaJ"
                     /protein_id="HMB45862.1"
                     /translation="MNTKVIFHCLGISQKNYFKNFVIPVIILAILFPVIILILVPSVM
                     QGIMVVGVILVPIALFAIVLIYPVSGLEGKKKEIDNNIHYYITHMGVLATSQMTRVDL
                     LLKLSHTEAYGYLAKETGRIHALIFYWHVSFPVACRFIAQRTPSVLFSDFLDRMAHSV
                     QAGQGFEEFVISEQTVVMNDFTTMYQDSLNSIDMIKEMFISMCMSLIFIVAFAIIMPI
                     ITGIDSILLLGGAIAIFIGMELVILLYARSKVPADRIWHTLEIETVADRRIKWSMPIS
                     LLLCIIVTGLVLVFTKLPTTIMFAASLTPLLLTGLIARTEENKIKRYDNNFGAFIRSL
                     GGAAGSRSGLILESLKELTSHDFGPLSENVNNLYKRLKTRLNTMRSWEHFAAGTGSNL
                     IERFATMFVEGTNVGGKPAIIGDIISKNFMSILSMRKLRYSSASSLIGVLYGMTAGIA
                     ATMFLALSIIAMLAKMFSNISIPDIDIGISIATISATNIPLMTTMLMIMMVCHSLMSA
                     MLIRIVDGGHHFNTYTHFVGLVWISALTAELTMQGMGPLLGM"
     gene            complement(2304..>2731)
                     /locus_tag="VKL21_08580"
     CDS             complement(2304..>2731)
                     /locus_tag="VKL21_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45863.1"
                     /translation="KLLQRLSGEPINIPASFADNLNAALILQAIYRKGKYLRRCTSVD
                     EIEGYLEEAGGVITRGVFEWDFTNDKHRFRGMNNSFLLDKIAVKQGYDDKRLIYDDLA
                     LRAKILNRMVEENIMDYYKVRDIIWAYQARGLEGIPFEV"
BASE COUNT          800 a          606 c          486 g          839 t
ORIGIN      
        1 tgactacatc gggccttagc ttggtgacct tttcaatagc ttcctgtccg tttttcgcct
       61 tgccgattac ttcaatatcg ggggactctg ccagaatatc agtgacgatt ttacgcatga
      121 aagctgaatc atcaacaaca agcactctga tcctggacat ttttaacctg atcacatatt
      181 cttggataca gcttccagta cttttgcagc gtcaaatggt ttaatgataa aatcccttgc
      241 gcctattttg atcgcatcat tcacaacagt ctgctgtcct attgaactgc acataataac
      301 ttttgcatta ctatcaaaag ccattatttg tttgagacat tctattccgt caatgttggg
      361 cataactatg tccaggaata caagattggg tttatgctcc ttgtactttg caacaccgtc
      421 tgaaccatcg actccttcaa ccaattcgtg tcctgttggt gaaatgattt tcttaagcat
      481 cattcgcatg aatgccgcat catctaccag caatattttt cctgccattc ttcctccaaa
      541 taatattttt taaatttata tatttcaaag ttcatttcat tatttaagtg ataatcttta
      601 tcacattggt atatacgaaa gtgtgatgtt tgtgtgaaat tgcatgatta cattcccaac
      661 aacggcccca tcccctgcat cgttaattcg gctgtcaacg cagaaatcca cacaagccct
      721 acaaaatggg tgtatgtatt aaaatgatga cctccgtcaa caattcttat tagcatcgca
      781 gacattagcg aatggcaaac catcattatc ataagcatgg tggtcatcaa tggaatattg
      841 gtggccgaga tcgtagcaat tgaaatccct atatcgatat cgggaatcga aatatttgaa
      901 aacatttttg ccagcattgc aattatggac aaggcaagaa acatagtcgc tgcaattccc
      961 gctgtcatgc catagagcac gcctataagg cttgaagcgc tgctgtatct caattttctc
     1021 attgaaagta tactcataaa attcttgctg attatatctc cgataatggc cggttttcct
     1081 cctacatttg ttccctccac gaacattgtt gcaaagcgtt ctataaggtt actccctgtt
     1141 cctgctgcaa aatgctccca tgaacgcatg gtattgagcc tggttttaag tctcttatac
     1201 aggttattta cattttctga aagtgggccg aaatcatgtg aagtcaattc tttcaacgat
     1261 tcaagaatta gtccgcttcg tgacccggca gccccgccaa gagaacggat gaaagcgcca
     1321 aaattattgt catatctctt gatcttgttc tcttcggtcc gcgctatcag tccggtaagc
     1381 aatagtggag ttaatgaagc tgcgaacatg atagttgttg gcaatttagt gaaaacaagt
     1441 acaagtcccg taacgattat acaaagcaat aaagaaatcg gcatagacca cttaattctg
     1501 cgatcagcta cagtctcaat ttcaagtgta tgccatatcc tgtcggctgg cacttttgac
     1561 cttgcataca acaatatcac taattccatg ccaataaata tggctattgc gccgccaagc
     1621 agaagaatag aatctattcc ggtaattatc ggcataataa tggcaaatgc cacaatgaat
     1681 ataagtgaca tgcacattga aatgaacatt tcttttatca tatcgatgga attcaaagag
     1741 tcctggtaca ttgtcgtgaa atcattcatt accacggtct gctctgaaat aacaaattcc
     1801 tcaaatccct gcccagcctg gaccgaatgc gccatcctgt caagaaaatc tgaaaaaagc
     1861 accgatggcg ttcgctgtgc tatgaacctg cacgccaccg ggaaacttac atgccagtag
     1921 aaaataaggg catgtatcct cccggtctct tttgccagat aaccataggc ttcggtatgt
     1981 gaaagcttga gcaacagatc aacccttgtc atctgtgagg ttgcaagaac gcccatatgt
     2041 gtaatataat agtgaatatt attgtctatt tcttttttct tgccctcaag gcctgatacg
     2101 ggatatatta aaacaattgc aaaaagcgca ataggtacaa gtattacacc cacgaccatt
     2161 attccttgca ttactgaagg gacaagaatc aggattatga caggaaataa aatcgctaaa
     2221 ataattaccg gtattacaaa attcttaaaa tagttttttt gcgaaatccc aaggcagtga
     2281 aatatgactt ttgtgttcat atttcatacc tcaaatggaa tgccttcaag acctcttgcc
     2341 tgatatgccc atattatatc ccttacttta taataatcca tgatattttc ttcgaccatc
     2401 ctgttaagga ttttggcgcg taatgcaagg tcgtcatata tcaggcgctt atcatcataa
     2461 ccctgtttga ctgctatttt gtcaaggaga aaactattat tcatacccct gaaacgatgc
     2521 ttgtcatttg tgaaatccca ttcaaaaact ccccgggtta ttacaccgcc cgcttcttcc
     2581 agataccctt caatttcatc aaccgatgtg catcttcgta aatattttcc ttttctatag
     2641 atcgcctgaa ggatcaaagc cgcattgagg ttatctgcaa atgatgccgg gatattgata
     2701 ggttcaccgg aaagtctctg aagaagtttt t
//
LOCUS       DATGBN010000350         6428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_532379, whole genome shotgun sequence.
ACCESSION   DATGBN010000350 DATGBN010000000
VERSION     DATGBN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532379"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VKL21_08585"
     CDS             <1..302
                     /locus_tag="VKL21_08585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HMB45864.1"
                     /translation="EASSVHKKLQEHGYGIIGTSSRIDEAVDSYEILVEKIILAAEIE
                     TTMKKLLEDIEKTKRRVNALEFKVIPELGEARDFIVLRLEEMERENTFRLKRIKG"
     gene            complement(313..834)
                     /locus_tag="VKL21_08590"
     CDS             complement(313..834)
                     /locus_tag="VKL21_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMB45865.1"
                     /translation="MMTSEDLIRAAMESDEAFIKEFQRTIKEELHSTAADFSEKSGIS
                     ASTLYKLLSGQREPNIKTLRQIIAVLRKIEGTDKPEFIAVIAARPVLDYITEKKLKVA
                     GRLLTIREYSATSMEEAIIAAVKAERDGAKALVCAPIVSTTVEKILRIPVTSIMPKDS
                     LIEAIELAARKMG"
     gene            complement(1093..2079)
                     /locus_tag="VKL21_08595"
     CDS             complement(1093..2079)
                     /locus_tag="VKL21_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45866.1"
                     /translation="MKKVRASMGTLGTLGLELVQMDTPPTTAYLQIYTEKRCKANCQF
                     CAQASGSSADLTHIARGMYIPFDLEAVVERLKMAYEREYLARACIQTLLYDTWWEDTV
                     YLVKRIRKESQIPISLAVFPLDTGKYEELRKMGVNELVVPLDACTPELFDKIKGKTTG
                     GPYSWERHIDGIKLASVIFEKVGTHLIIGLGESDEDAIKIISGFFNNNVNTALFSYTY
                     IPGTQSKASDKTENESVRHYRTVQLARHLILEKLSSYPGMMFKEGVLIDYGVNADVIH
                     QTIENGKAFQTSGCPGCNRPMANETFSKILNFPKKPADVEKHNIKKDLGLIV"
     gene            complement(2094..2306)
                     /locus_tag="VKL21_08600"
     CDS             complement(2094..2306)
                     /locus_tag="VKL21_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45867.1"
                     /translation="MAVKKYSEGAITIEIDEDKCNGDGECVNVCPTNVFELVDGKSKA
                     TRVADCIECCACVDACPTKAIKHNSC"
     gene            complement(2356..2514)
                     /locus_tag="VKL21_08605"
     CDS             complement(2356..2514)
                     /locus_tag="VKL21_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45868.1"
                     /translation="MEDIEMVLEINGTKIPMNGFVKKILCGMVKGSIETLHGVGDDWK
                     DVNIRMSR"
     gene            2615..3301
                     /gene="radC"
                     /locus_tag="VKL21_08610"
     CDS             2615..3301
                     /gene="radC"
                     /locus_tag="VKL21_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMB45869.1"
                     /translation="MQEYKIKILDMQKDERPRERLIKNGASALSDSELLAIILRTGSK
                     QENVINLSQRILKEYNIKQLSQTNLKQLMRIHGIKESKAAQIAACFEIARRLESFKDE
                     DKQKISSPEDVYRRLYPKMREQKKELFIELCLDTKNRIIREEIISVGSLNANIVHPRE
                     VFKLALAESAAHIIVAHNHPSGDPAPSREDIEITKKLAQTGIIMGITLLDHVIIGDGR
                     HFSMKEAGHI"
     gene            3315..3914
                     /locus_tag="VKL21_08615"
     CDS             3315..3914
                     /locus_tag="VKL21_08615"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HMB45870.1"
                     /translation="MAYSAIILAGGKGKRMGHREKALMAFNGKPLISYVIKSLEKVVD
                     DIIISVRDKAQEELLIPVLPGYTYVSDAYENAGPLSGILSSLTVCRDDFCFIAACDMP
                     FINENVVELLFKKSEDHDAAIVRREDGRLEPLHAVYKCKPMVFETMRAFKNGKTKILA
                     PIHRMKVNYVEIEEIRNIDPDLKTFININTLEDLNRNCQ"
     gene            3930..4682
                     /gene="fdhD"
                     /locus_tag="VKL21_08620"
     CDS             3930..4682
                     /gene="fdhD"
                     /locus_tag="VKL21_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HMB45871.1"
                     /translation="MYTKNYPALELKGGLSHKKTHTVAVENTIELFINKIPIASILST
                     PEMLKELAIGYLICEGLVKNPDSIRNFKIDENRIYAEIEQSEHLELWRELRSSGCVGV
                     RWEENENISIDSDTKFSAYSIEKSLRFLESDIYRETHGTHAACLINVRGECVAKAIDV
                     GRHNAFDKVVGYAYLNGIDVKEHFIISTGRQSAGMVLKAARAGIPLVATKTAPLNSGI
                     DATHRSGVCLVCFVSNERISIFSHPERLLCRL"
     gene            4670..5131
                     /gene="mobB"
                     /locus_tag="VKL21_08625"
     CDS             4670..5131
                     /gene="mobB"
                     /locus_tag="VKL21_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HMB45872.1"
                     /translation="MPPVICIVGTSKTGKTKLMEVLIKDLKNRGLKVGALKYHKHGDF
                     EIDVEGKDTWKYAKAGADTVAITSSVKFAVIKNENVMTDIDEICETYFYDTDVVLADG
                     FTQSDKPRIIVVENEKDAGIFKRGCRVISITDDKIKDMKSVFQKVHEFLNA"
     gene            5167..5535
                     /locus_tag="VKL21_08630"
     CDS             5167..5535
                     /locus_tag="VKL21_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45873.1"
                     /translation="MDYWFGNFINDYILYKIIEGIIAILSMIVIYLGIHIALNWKFIN
                     KEIPDSDEIISQKQSFYRSTLFIFIAGFFMIIHEFFEGLEKEAPDFATYELLELVALS
                     GLVMFLYEWHKILMKLKKSR"
     gene            5621..5920
                     /locus_tag="VKL21_08635"
     CDS             5621..5920
                     /locus_tag="VKL21_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMB45874.1"
                     /translation="MEETKFCSKCGEKIDIEAEICPKCGVRQALPQSSTKEKKPPEPW
                     LAALASFLIPGLGQLYTRQSFGKAALLFCTSWLILPWIYSIYDAYKTAEEMNEKQ"
     gene            5907..6413
                     /locus_tag="VKL21_08640"
     CDS             5907..6413
                     /locus_tag="VKL21_08640"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HMB45875.1"
                     /translation="MKSNNSRTSFSIFSNLKRVIDEVGEERVLALIAHHEPDQFYKTI
                     KFSIFNKNIRFCTRCIGIYFGIIISVLYLFFSLTTPEIFVFGIMFAFPIPAMLDWGLE
                     KYSIWKGNNATRFVSGFMLGICYVFLWTKFIQNPFDIKVWLIAIVYVVIAYFVLKFSS
                     KKVVWREY"
BASE COUNT         1959 a         1211 c         1344 g         1914 t
ORIGIN      
        1 tcgaggcatc aagcgttcat aagaaacttc aagagcatgg ttacggaatt ataggcacaa
       61 gctcacgcat tgatgaagct gttgattctt atgaaatact tgttgagaag atcatccttg
      121 ccgccgagat cgagaccaca atgaaaaaac tgcttgagga tatcgagaag accaagcgca
      181 gggtaaatgc ccttgaattc aaagtcatcc cggagcttgg tgaagccagg gatttcattg
      241 ttttgaggct tgaagaaatg gaaagggaga ataccttcag gctaaagagg ataaagggct
      301 aataacctta attcatccca tctttcttgc tgcaagttca atcgcttcaa tgaggctgtc
      361 ttttggcata atagaagtaa ccgggatgcg aaggattttc tcaacagtag tgctaacaat
      421 aggagcgcat acgagtgctt tcgcgccgtc cctttcagct tttactgcgg cgatgattgc
      481 ttcttccatg gaggttgcag aatattctct gatcgtgagg agcctgccgg caactttcaa
      541 ttttttctca gttatataat caaggacagg tcgggctgcg ataacggcta taaattcagg
      601 tttatccgtt ccttcaattt tacgaagcac agcgattatt tgcctcagtg tcttgatatt
      661 gggctcgcgc tggcctgata ataatttata taatgtactt gcagagatac ctgatttttc
      721 actgaaatcc gctgctgtgg aatgaagttc ttccttaatt gttctctgga attccttgat
      781 gaaagcttca tccgattcca ttgctgcccg tatcagatct tctgaggtca tcacaattgt
      841 gtatattgta ttcgttatta atacttttgg gtcaatagaa aacaccgaac taatacgaac
      901 tgttacaaac tccggttgat tttgttatca ggaaattggt tgggcagttt ttccaaatta
      961 ataaaaatca gacaggattt acaggatcga caggattgga ttcacaagtc atcctgtaaa
     1021 tcctgttatc ctgtcgatta gaaaaaaaag aattttatcg aatttgccac agtctcacct
     1081 aacatggaaa attcaaacta tcaatccaag atccttcttt atgttgtgtt tttcaacatc
     1141 cgcaggtttt ttcggaaaat tcaatatctt tgaaaatgtt tcattcgcca tgggcctgtt
     1201 acagccagga catccggaag tctggaaagc ttttccattt tcaatagtct gatgaataac
     1261 atctgcattc actccgtaat caataagaac tccttcttta aacatcatgc caggataaga
     1321 tgagagcttt tcaaggatca agtgcctggc aagctgcaca gttcgataat gcctgacgct
     1381 ttcattttcg gttttatccg atgcttttga ttgtgtcccc ggtatatagg tatatgaaaa
     1441 cagcgctgta ttcacgttat tattaaaaaa acctgaaatt atcttaattg catcttcatc
     1501 gctttcccca agacctatta tcaggtgcgt ccccactttt tcaaaaataa cggatgccaa
     1561 ttttatgcca tcaatgtgcc tttcccatga ataaggtccg cccgttgttt ttcctttaat
     1621 cttatcgaat aattcaggcg tgcaggcatc aaggggcacg acaagttcat tgactcccat
     1681 ttttctcaat tcttcatatt ttccagtatc caggggaaaa acagccaggg aaatgggaat
     1741 ctggctctct ttgcggatcc tttttacaag gtaaaccgta tcttcccacc aggtatcata
     1801 aagtaatgtc tgtatgcagg cgcgggcaag atattcccgc tcataagcca tcttaagccg
     1861 ctccactaca gcttcaagat caaaaggtat gtacatgccg cgcgcaatat gagtcaggtc
     1921 tgcggatgag ccggatgcct gggcgcagaa ctggcaattt gccttgcagc gtttttcggt
     1981 gtatatctga agatatgccg tagtgggcgg tgtatccatc tgcacaagtt caagcccgag
     2041 tgtgcccagg gttcccatcg aagccctgac ctttttcatg cttccagccg aggtcaacag
     2101 gaattatgct tgatcgcctt tgtgggacat gcatcaacac atgcgcagca ttcgatgcaa
     2161 tctgcaaccc gcgtggcttt tgatttgcca tctacgagtt caaaaacatt agtagggcaa
     2221 acattaacac attcgccatc gccattgcac ttatcttcat caatttcaat cgttattgcg
     2281 ccttcactat atttcttaac tgccattttt ttctccaatt cggtatgtgt tattattcaa
     2341 gattaaatgt tttggttacc tgctcattct gatatttaca tccttccaat cgtctcccac
     2401 cccgtgaagg gtttcaattg agcctttgac cattccacaa agtatttttt tcacaaaacc
     2461 attcataggt atttttgtcc cgtttatctc aaggaccatt tcgatatctt ccatattatt
     2521 tctccatcct cattatgtca gattctcaat atagttctat tgtttgatca ggtctgggaa
     2581 gattttatgc atatgaaaag gattatactg gcaaatgcag gaatataaga taaagatcct
     2641 tgatatgcaa aaagacgaaa ggccgcgcga gagactgata aagaacggcg cgtcggcatt
     2701 gagcgattct gaacttcttg caataatctt gaggacgggt tcaaagcagg aaaatgttat
     2761 aaacctctcc cagaggatct taaaagaata taatattaaa cagctctcac agacaaatct
     2821 caaacaatta atgaggatac atggtatcaa agaaagtaag gccgcgcaga tcgcagcctg
     2881 ttttgagata gcgcggcgcc tggagtcttt taaggatgag gacaaacaaa agatcagctc
     2941 tcctgaagat gtttacaggc gtctctatcc gaaaatgagg gagcagaaaa aagaactttt
     3001 tatcgagcta tgtcttgata caaaaaaccg gataatcagg gaggaaataa tttctgtggg
     3061 ttcattgaat gcaaatatcg tgcatcccag agaagtattc aaacttgcat tagcagaatc
     3121 ggcagcgcat ataatagttg cccataatca tccctccggc gatccggcgc caagcaggga
     3181 agatattgaa atcacaaaaa aattggcgca aaccggaata attatgggga tcactcttct
     3241 tgaccatgta ataattggcg acggccggca tttcagcatg aaagaggcgg gacatatata
     3301 aaacagggag taaaatggca tattctgcaa ttatccttgc cgggggaaaa ggaaaacgca
     3361 tgggtcaccg cgaaaaagct cttatggcgt ttaatggcaa accccttatc tcttatgtta
     3421 tcaagagcct tgaaaaagtg gttgatgaca tcataatttc cgttcgtgat aaagcgcagg
     3481 aagagttgtt gatcccggta ttgccagggt acacttatgt atctgatgct tatgaaaacg
     3541 caggtccgct ttcgggaata ctttcaagcc tcacggtttg cagggatgat ttttgtttta
     3601 tcgccgcatg cgatatgcct tttatcaacg aaaatgtcgt agaattgctt ttcaagaaaa
     3661 gtgaagatca cgatgctgcc attgtgcgcc gtgaagatgg ccgtcttgag ccgctgcatg
     3721 ctgtttataa atgcaaaccc atggtttttg agacaatgag ggctttcaag aatggaaaga
     3781 caaaaatcct tgctcccatt catagaatga aggtcaatta tgtggaaatt gaagaaatca
     3841 ggaacattga tccggattta aaaacattta tcaatatcaa tacactagaa gatttgaatc
     3901 ggaattgtca ataatttatc atcttaacaa tgtatacaaa aaattatcct gcacttgagc
     3961 tcaaaggcgg tctatcgcat aaaaaaaccc atacagtagc tgtagagaat actattgagc
     4021 tttttataaa caaaatcccc atcgcgtcaa tactttcaac gccggaaatg ttaaaagaac
     4081 tggccatcgg gtatctcata tgcgaaggtc tggtaaaaaa cccggatagc ataaggaatt
     4141 tcaaaattga tgagaacagg atatatgccg aaattgaaca atccgaacat cttgaactct
     4201 ggcgggaatt aaggtcatcg ggatgcgtag gtgtaaggtg ggaggagaat gagaatattt
     4261 cgatcgattc ggatacaaaa ttcagtgcat attcaattga gaaaagcctc agattcctgg
     4321 aatctgatat ctaccgggaa acgcatggga cgcatgcggc atgtctcata aatgtaaggg
     4381 gggaatgcgt ggcaaaagcc atagatgtag gaagacataa tgcttttgat aaagttgttg
     4441 gatatgcata tcttaatggg atagatgtga aggagcattt tatcatatcc acgggacgcc
     4501 agtcggcagg catggtgttg aaagctgccc gggccgggat cccgcttgtt gccacaaaaa
     4561 ccgctcctct taattccggt atcgatgcca cccataggag cggagtatgt cttgtgtgtt
     4621 ttgtttcaaa tgaacgaata tcgatttttt cgcatcctga gaggcttcta tgccgcctgt
     4681 aatttgtatt gtggggacaa gcaagaccgg aaaaacaaaa ttaatggaag tattgataaa
     4741 agacctcaag aatcgcggcc tgaaggtagg cgccttaaaa taccataaac acggagattt
     4801 tgaaatagat gtagaaggca aagatacatg gaaatatgca aaggctggag ctgataccgt
     4861 tgccataacc tcatccgtga aatttgctgt tatcaaaaac gaaaatgtaa tgactgatat
     4921 tgacgagatc tgtgaaacat atttttatga cactgatgtt gtacttgcgg acggattcac
     4981 tcaatccgat aagcccagga tcattgtggt tgagaatgag aaggatgcgg gaattttcaa
     5041 aaggggatgc agggtcatat cgattacgga tgataagatc aaggatatga aaagtgtttt
     5101 tcaaaaagtg catgaattct tgaacgcatg aaaaaattat tatagtgcca atcccaatac
     5161 ttcaacatgg attattggtt cggcaatttc atcaatgatt atatccttta caaaataatt
     5221 gaaggaatta tagcgatact ctcaatgatt gtgatttatt tgggtatcca tatagctttg
     5281 aattggaaat tcataaacaa ggaaattccg gattcagacg aaataatctc acaaaaacag
     5341 agtttttatc gaagtacact gtttattttt attgccggtt tttttatgat tatacatgaa
     5401 ttttttgaag gccttgagaa agaagcgccc gatttcgcga cttatgaatt gctggagctg
     5461 gtcgcacttt ccggattggt catgttttta tatgagtggc ataaaatatt gatgaaatta
     5521 aaaaaaagta gatagataag taatatcctt agcttatgcc aaaatttttg tggatatatt
     5581 taaatatata ctggaataat aagaccagaa aaggtgagtt atggaagaaa ctaaattttg
     5641 cagtaaatgt ggtgaaaaaa ttgacatcga agccgaaatt tgtccaaaat gtggcgttag
     5701 acaggctttg ccacaatcct caacaaaaga gaagaagcca cctgagccat ggcttgcagc
     5761 tttagcgagt tttttgattc cgggattggg acaactatac accagacaat cgtttggtaa
     5821 agcggcactt ttattctgca catcttggct tattctacca tggatatata gtatatacga
     5881 tgcttataag acagcagaag aaatgaatga aaagcaataa ttcaagaaca tctttttcca
     5941 ttttttctaa ccttaagagg gtgattgatg aagttggaga ggagagagtt cttgcattga
     6001 ttgcacatca tgaaccggac caattctata agactataaa attttcaatt tttaataaaa
     6061 atatacgatt ctgcacacgt tgtataggaa tttattttgg aattattata tctgttttat
     6121 atttattctt ttcattgacc actcctgaaa tttttgtatt tgggatcatg tttgctttcc
     6181 caattcctgc aatgctggat tggggacttg aaaaatatag tatctggaaa ggcaacaatg
     6241 ccactcgttt cgtttccgga ttcatgttag gaatttgtta tgtcttccta tggactaaat
     6301 tcatccaaaa tccatttgat attaaagttt ggttgatagc cattgtatac gttgtcatag
     6361 catattttgt acttaaattt tcttcaaaaa aggttgtttg gagagagtat taaaaaaatt
     6421 ttacttaa
//
LOCUS       DATGBN010000351         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_454766, whole genome shotgun sequence.
ACCESSION   DATGBN010000351 DATGBN010000000
VERSION     DATGBN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454766"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VKL21_08645"
     CDS             <1..556
                     /locus_tag="VKL21_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45876.1"
                     /translation="ALRHQYLYEKEKIKKNRNYNILVPLGMLNDSVKLINFLYKSPIN
                     TSKYNIIIRCHPIVNLEQIRKRLNFNHDEKFSVSSIANIKEDLRRVDIVIYTSTTVCI
                     EALMMGIPVIHIDLNEPITIDPLFRMNDLKWVVTKEEELPMIIENIYALDGLELSELQ
                     DKARKAIQDYLSEVNEDKLKEFIE"
     gene            604..1377
                     /gene="rfbF"
                     /locus_tag="VKL21_08650"
     CDS             604..1377
                     /gene="rfbF"
                     /locus_tag="VKL21_08650"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HMB45877.1"
                     /translation="MKIVILCGGFGTRLREETEYKPKPMLEIGDMPILWHIMKIYSTY
                     GFNEFVLCLGYKGEMIKDYFYHYKMRNNDFSMDLKDGNITLFDSKEENDWNIILANTG
                     LNSMTGARIKRIEKYIDTDQFMVTYGDGVTNLNISDLLKFHQKHNKIGTVTGVYPPSR
                     YGELRIEGDRVVSFDEKPDTGVAPISGGYFVFNKEFFDYLKTDESCVLEKEPLTNLAK
                     DEQLKVYNHRGFWQCMDTYRDYTFLNDLWKSNKAQWKVW"
     gene            1378..2349
                     /locus_tag="VKL21_08655"
     CDS             1378..2349
                     /locus_tag="VKL21_08655"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMB45878.1"
                     /translation="MQWKNTSVLVTGCTGLLGSWLCKELIARKANVVGLVRDWVPKSV
                     LINDNYIDSMNIARGDVTDFHIVERVLNEYEVEIVFHLAAQTIVEIANKNPLSTFETN
                     IKGTWNILEASRRIPTVKKIIIASSDKAYGDQKELPYHEDTPLQGTHPYDVSKSCADL
                     IAKTYHITYGTPVCITRCGNFYGGGDLNFNRIVPGTVRALLNDEQPIIRSDGSYIRDY
                     FYVEDGVHAYLHLAEKMDDTKILGEAFNFSNELQITVLELVNKMIALMDKEKIEPKVL
                     GIAKNEIIHQYLSAKKARKMLGWAPKYSLDEGLNRTIEWYTRFFMEE"
     gene            2351..2800
                     /locus_tag="VKL21_08660"
     CDS             2351..2800
                     /locus_tag="VKL21_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HMB45879.1"
                     /translation="MIQGVEINPLAIIPDERGMILKMLRRDDKFFQEFGEIYFSLIYP
                     GVVKGWHIHKEMTLNYAVISGNIKLVLYDDRDDSPTKGDIQEIYLGRENYKLVTIPPM
                     VWNGFKGVGIESAIVANCATIPHNPDEIDRMDPFNNKIPYDWELKHR"
     gene            2797..>3086
                     /locus_tag="VKL21_08665"
     CDS             2797..>3086
                     /locus_tag="VKL21_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMB45880.1"
                     /translation="MIILITGGFGYIGGRVAQHLGKIYGDENILLFVKNNKIKPDWVG
                     DKKILTGDVLDISSLEAACKDIDVIIHLAALNEIDSERNPLKALRINGEGTL"
BASE COUNT         1095 a          528 c          651 g          812 t
ORIGIN      
        1 tgccttaagg caccaatatc tttatgaaaa ggaaaaaata aaaaaaaata gaaactataa
       61 tattctggtt ccattgggga tgctcaatga ttctgtaaaa ttaattaatt ttctatataa
      121 atctccaata aatacatcaa aatataacat aataatcagg tgtcatccaa ttgtaaattt
      181 agaacaaata agaaaaagat taaatttcaa tcatgacgaa aaattcagtg tatccagcat
      241 tgccaatatt aaagaggact taaggagagt ggatatagta atttatacca gcacaacggt
      301 ttgcattgaa gcgttgatga tgggaatacc agtaatacat attgatctta acgaacctat
      361 taccatcgat ccattgttta gaatgaacga tttaaaatgg gtagtaacta aagaagaaga
      421 acttccaatg atcatagaaa atatatacgc tcttgacggc ttggaattat cagaattaca
      481 ggataaggca cgaaaagcta ttcaagatta tttgtctgag gttaatgaag ataaactgaa
      541 ggaatttatt gaataagcaa taacattttt atagaataca ctatataaac aacaaagaaa
      601 atcatgaaaa tcgtaatcct gtgcggtggc tttggcacac gcctccgaga agaaacagaa
      661 tacaaaccca aacccatgct cgaaataggt gacatgccga tactctggca tataatgaaa
      721 atatattcca cctatggttt caatgagttc gttctctgcc ttgggtataa aggtgaaatg
      781 ataaaggact atttttatca ttataaaatg agaaacaacg atttttcaat ggatttgaag
      841 gatggcaata tcaccctatt tgactcaaaa gaagagaatg actggaatat catccttgcc
      901 aacactggtt tgaattcgat gacaggtgca aggatcaaaa ggatagaaaa atacatagac
      961 acagatcagt tcatggtaac ttacggcgat ggcgtcacca acctgaacat ctcagacctc
     1021 ttaaaattcc atcaaaaaca caacaagatc gggactgtca caggtgtata tcccccctcc
     1081 cgatatggag aactccgaat agaaggcgac agggttgtct cgtttgacga aaaaccggac
     1141 acaggagttg cgcccatcag cggaggatac tttgtgttca ataaagagtt ctttgattac
     1201 ctgaaaaccg atgaatcctg tgtacttgag aaagaaccgc tgacgaatct tgcaaaggac
     1261 gaacagttaa aagtctataa ccacagggga ttctggcagt gcatggatac ctacagggat
     1321 tacacgttcc tgaacgatct ctggaaatca aacaaagcgc aatggaaggt atggtaaatg
     1381 caatggaaaa acacatctgt actcgtcaca ggttgcaccg ggcttctggg ctcatggctt
     1441 tgtaaagaac tcattgccag aaaagccaat gttgtgggac ttgtgcgtga ctgggttcca
     1501 aagtcagttc ttatcaatga taattatata gacagcatga atatcgcaag aggggatgtc
     1561 acggattttc atattgttga aagagtgctc aatgaatatg aggttgagat agtttttcat
     1621 cttgctgcac agaccatcgt ggaaatcgca aacaagaacc ctctttctac attcgaaaca
     1681 aacatcaaag gaacatggaa tattcttgaa gcttcaagaa gaattccaac tgttaaaaaa
     1741 ataataatag cttcaagcga caaagcctat ggagatcaga aagaacttcc ataccatgag
     1801 gatacgcctt tgcagggcac tcatccttat gatgtatcca aaagctgtgc tgatttgatc
     1861 gcaaaaacct atcatatcac ctatgggaca cctgtatgta taacacgctg cgggaatttt
     1921 tacgggggag gagacctgaa cttcaacagg atagttccgg ggactgtccg ggctttactg
     1981 aatgatgagc agccaataat aaggagcgat ggttcatata taagagatta tttctatgta
     2041 gaagacgggg tccatgcata tctgcacctt gctgaaaaga tggatgatac aaaaatattg
     2101 ggagaagctt tcaatttcag caacgaactc cagataacgg tgcttgaact tgtgaacaaa
     2161 atgatcgcgt taatggataa agaaaaaatc gagccaaagg ttcttggcat tgcaaagaat
     2221 gagatcatac atcaatattt atcagctaaa aaggcaagaa aaatgcttgg ctgggcgcca
     2281 aaatattccc tggatgaagg gcttaataga accattgaat ggtacacgag atttttcatg
     2341 gaggagtaaa atgattcaag gagttgagat aaatccactg gcaataatcc ctgatgaaag
     2401 agggatgata cttaaaatgc tgagaaggga tgataaattt ttccaggaat ttggggagat
     2461 atatttttca ttaatctatc ccggagtggt aaaagggtgg catatacata aagaaatgac
     2521 cttaaactat gctgtcataa gcggaaacat taaacttgta ctttatgatg accgggatga
     2581 ttctcctaca aaaggcgata tccaggaaat ttatctggga cgggagaatt acaaactggt
     2641 gacgattcct ccgatggtct ggaacgggtt caaaggggta gggatagaat ccgcaattgt
     2701 agctaattgt gcaactatcc cccataatcc tgatgaaatt gatcgaatgg atccttttaa
     2761 taacaaaatt ccatatgact gggagctaaa acatagatga ttatccttat aacaggagga
     2821 tttggctaca tcggcggcag ggttgcacaa catcttggga agatatatgg tgatgaaaat
     2881 atcctgcttt ttgtcaaaaa caataagatt aaacctgatt gggttggtga taaaaaaatt
     2941 ctcacaggcg atgtgctgga tatatcatca cttgaagccg cctgcaaaga tatagatgtg
     3001 ataattcatc tggcagcttt aaatgaaatt gattctgaaa gaaatcctct aaaagcattg
     3061 aggataaacg gagaggggac tttgaa
//
LOCUS       DATGBN010000352         5249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_299479, whole genome shotgun sequence.
ACCESSION   DATGBN010000352 DATGBN010000000
VERSION     DATGBN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299479"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VKL21_08670"
     CDS             complement(<1..308)
                     /locus_tag="VKL21_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HMB45881.1"
                     /translation="MKDLSLFLGCVIPNRYPGIEKATRLILDRLGIKWSELKEASCCP
                     APGIFRSFDKVTWLTIAARNILLAEENNTDILTICNGCFSSLMDANNMLKNDEALRNE
                     V"
     gene            complement(308..748)
                     /gene="hdrC"
                     /locus_tag="VKL21_08675"
     CDS             complement(308..748)
                     /gene="hdrC"
                     /locus_tag="VKL21_08675"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HMB45882.1"
                     /translation="MKNSEILTCFQCGTCTGSCPSGKYTSLNVRRIIKDSIKKDISDQ
                     LDLWMCTTCYNCQERCPRGIKVTDAILSLRAKAVKNGRILPAHRAVCKFLIEMGHAVP
                     IDDIHIAIREKIGLAQPETVSKYPEALKDVKTLLASTGFDGLIK"
     gene            complement(850..1164)
                     /gene="rpl12p"
                     /locus_tag="VKL21_08680"
     CDS             complement(850..1164)
                     /gene="rpl12p"
                     /locus_tag="VKL21_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HMB45883.1"
                     /translation="MEYVYAALLLHSAGKKVSEEGITAVIKAAGVDVDAVRAKALVSA
                     LDGVNIEEAISKAAFAAPAAAAPAAAAAASPAAAEAPKADEKTKEKTEESGMEGLGAL
                     FG"
     gene            complement(1186..2205)
                     /locus_tag="VKL21_08685"
     CDS             complement(1186..2205)
                     /locus_tag="VKL21_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMB45884.1"
                     /translation="MSTELAHQSIHIPKWKKDEIEDIKRLITTHSSIGIVGVHGIPSN
                     QLQLMRKSLRGIADLKMCRNSLIDRALDESSDDVKQINKYVEDQTALLFTNENPFKLF
                     KILDKGKTSAPIKGGTVSPKDIIVEKGPTSFPPGPIVGELTGAGIAAGIEGGKVVIRQ
                     TKTVAKKGDVVDAKLASILSRLEIHPMELGLDLRAVYENGMIYESSILSVDETTYTNN
                     LMLAVQRAFNLSVNSAYPAKATISTLLTKAASQSRNLAINADLIIPEIIDVLLGKANA
                     QMMSIAKLALGKDANSVSNKIKEKLAAAPEAKKPEAAKAAPAEVKKEEIKQEDIAAGL
                     GSLFG"
     gene            complement(2207..2848)
                     /locus_tag="VKL21_08690"
     CDS             complement(2207..2848)
                     /locus_tag="VKL21_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMB45885.1"
                     /translation="MEHEKILEAVTQAIEKSPQRKFSESVDLAINLKNLDMNQPTNRL
                     DEEIILPNGLGRPIKIAVFAKGDTAQRAKAAGADYVFDPEEISVLGEDKTRTKNLAEE
                     VTFFIAEAAYMPVIGKTLGQVLGPRGKMPIPLTPDKDVVQVINKSKNSIKVRSKDKMT
                     FHITVGKKDMDPVKLSQNIEAIINRIEHRYERGMYNVKSIYVKSTMGPAIKVV"
     gene            complement(2968..4176)
                     /gene="hmgA"
                     /locus_tag="VKL21_08695"
     CDS             complement(2968..4176)
                     /gene="hmgA"
                     /locus_tag="VKL21_08695"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HMB45886.1"
                     /translation="MNKELLEKVKRGELSFHDIDERLDPNASIELRRTAVSELTGTIF
                     NHISNYSLDVENVTKRNIENMIGAIQVPLGVAGPLRIKGEYARGDYYIPLATTEGALV
                     ASADRGSSVITASGGANVRIFKDEMTRAPVFRTKDIIGAKRLADWVDENFVMLKQKAQ
                     STTRFGELLSINAFTAGRNVFLRFSYDTKDAMGMNMVTIATDAAVDIILSENDVELVA
                     LSGNMCSDKKPAAINAILGRGKTVSADVFLVNETITDILKTTPEKMAEVNYRKNLLGS
                     ARAGSLGFNAHAANIAAAMFIACGQDAAHVVEASNAITTMELTDEGLYCSVTLPSLNV
                     GTVGGGTRIATQLECLDMMGVAGAGDPPGTNAKKFAEIIAAAVLAGEISLVGALSARH
                     LARAHAALGR"
     gene            complement(4173..4610)
                     /locus_tag="VKL21_08700"
     CDS             complement(4173..4610)
                     /locus_tag="VKL21_08700"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HMB45887.1"
                     /translation="MSLEEKLSYKFKDKNLLNRALTRKAYALEQKQQNIYCEDQEIFR
                     TLGDAVLKTVLIDLLIKNGVNSRGEITNRKKSLERQEGLAKISVRLGLGSYLKFGNGE
                     TKQRADKEPYVLAETFEAVIGAIYLDGGFDACRKTIEKLFEIL"
     gene            complement(4660..4893)
                     /locus_tag="VKL21_08705"
     CDS             complement(4660..4893)
                     /locus_tag="VKL21_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HMB45888.1"
                     /translation="MSNQVQPILPLAPVERIIRKAGADRVAEDAGVELAKVLEEYGIE
                     ISREAITLAKHAKRTTVKEEDIRLAVARIKKSS"
     gene            complement(4981..>5249)
                     /gene="cdc6"
                     /locus_tag="VKL21_08710"
     CDS             complement(4981..>5249)
                     /gene="cdc6"
                     /locus_tag="VKL21_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325497.1"
                     /note="Involved in the initiation of DNA replication;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division control protein 6"
                     /protein_id="HMB45889.1"
                     /translation="QLSYMMRTSKKEEKILLGLIADFPQSNSGDLYKKFHEKTALGYT
                     RFYELLNKLGSVKLIDADFTGKGARGRSRVVKLRYKPEEVKIRL"
BASE COUNT         1277 a         1228 c         1045 g         1699 t
ORIGIN      
        1 acttcatttc taagagcttc atcgttcttt agcatattat tggcatccat gagagaactg
       61 aagcagccat tgcaaatagt cagtatatcc gtgttatttt cttcggccag caggatgttc
      121 cttgctgcga tcgtgagcca agttactttg tcaaaagagc ggaatatccc gggcgccgga
      181 cagcatgaag cttcctttag ctcgctccat tttatcccca gcctgtccag gatcagcctt
      241 gtagcttttt cgatgccagg ataacggttc gggattacgc atcccaggaa gagagataag
      301 tctttcatta tttgatcagc ccatcaaaac ccgttgatgc aaggagtgtt tttacatcct
      361 ttagcgcttc aggatacttg ctaactgttt ctggttgcgc aagccctatt ttttccctga
      421 tggcaatatg aatgtcatca atgggaacgg catgtcccat ctcaataaga aatttgcata
      481 cagccctgtg agcaggcaat atcctgccat ttttcacagc ttttgcacga agggaaagga
      541 tggcatctgt tacctttatt ccgcgcgggc atctttcctg gcaattgtag caggtagtgc
      601 acatccacag atcaagctgg tcagagatgt ctttttttat tgaatcttta ataattcttc
      661 ttacattgag gcttgtatac ttccctgaag ggcagcttcc tgtacaggtg ccgcactgga
      721 aacaggtgag tatttcagaa tttttcatta aaggaattat gtggatactt gtatttaaga
      781 ctttaaggga gtgaaatatt cgaaaggaac aaaaaaaaga aaaaaattta agaagaatat
      841 gaattcttct taaccgaaca aggcgccgag gccttccatt ccgctttctt cagttttctc
      901 tttggttttt tcatctgcct ttggagcctc agctgccgcc ggggatgccg ctgcggctgc
      961 tgctggagct gctgctgccg gggctgcaaa tgctgccttt gatatggcct cttcgatatt
     1021 gacaccatca agggctgaaa ctagcgcttt tgcccttact gcatcaacat caacacctgc
     1081 tgctttgata acagcagtta ttccttcttc agacactttc tttcctgcgc tgtgtaataa
     1141 tagcgctgca tatacgtatt ccattttaat caccttttat tgaatttaac cgaataacga
     1201 gcctaagccc gctgcaatat cttcctgttt tatttcttct ttctttactt ccgcaggagc
     1261 agcttttgca gcttcaggct tctttgcctc tggcgctgcc gcaagctttt cctttatttt
     1321 attactgacg gaatttgcat ctttccctaa tgccaatttt gcaatagaca tcatctgtgc
     1381 atttgctttt ccaagcagta catcgatgat ttccggtatt atcagatcag catttattgc
     1441 aagattcctt gactgggatg ctgcttttgt aagcagtgtg ctgatcgtgg cttttgccgg
     1501 ataagctgag ttgactgaca aattaaatgc cctctggacc gccaacatca gattattagt
     1561 atatgtcgtt tcatcgaccg ataagatgct agactcatat atcatgccat tttcgtaaac
     1621 agcacgcagg tcaagcccca attccatagg gtgtatttca agacgggaaa ggatagatgc
     1681 gagttttgca tcaactacat cgcctttctt tgcaacggtc ttggtctggc ggataactac
     1741 ttttccacct tcgatacctg ctgcaattcc tgctcctgtc aattctccca ctatcggccc
     1801 gggagggaaa gaagttggtc ctttctcgac tattatatcc tttggcgaga cagttccgcc
     1861 tttaattggc gcagatgtct tccctttatc cagtatctta aagagtttga aaggattctc
     1921 gtttgtaaat aatagcgcgg tctggtcttc aacatatttg tttatctgct taacatcatc
     1981 ggatgattca tcaagtgcgc gatcaataag agaattcctg cacatcttga gatccgcaat
     2041 accgcgcagg ctctttctca tcagttgaag ctggtttgat ggaattccat gaactccgac
     2101 aataccgatt gacgaatgag tggtgatcag tctttttata tcctctattt catctttttt
     2161 ccatttaggg atatgaatac tctggtgtgc gagttctgtt gacattttat accaccttta
     2221 ttgcaggtcc cattgtggat ttgacataga tcgatttgac gttatacatg ccgcgttcgt
     2281 acctgtgctc aatacggtta attatcgcct cgatgttctg tgataatttc acagggtcca
     2341 tatccttttt acccactgta atgtggaatg tcatcttgtc cttggatctc acctttatgg
     2401 agttcttgga cttgtttatt acctggacca catccttatc cggcgtcaac ggtatgggca
     2461 tttttcccct gggaccaagc acctggccga gggttttacc gataacaggc atgtatgctg
     2521 cttctgctat gaagaaagtt acttcttctg caagattttt agttcttgtc ttatcttctc
     2581 ccagaacact tatttcttcg ggatcaaaaa cataatctgc accggcagct tttgccctct
     2641 gggcggtatc gccttttgcg aatactgcga tctttatagg tcttcccagg ccgtttggaa
     2701 gaattatttc ttcatcgagc ctgttcgtgg gctggttcat atcaagattt ttaaggttga
     2761 tagccagatc aacactttct gaaaattttc gctgtggaga cttctctata gcctgcgtaa
     2821 cagcttctag tattttttca tgttccaata taattcctcc cgtagttatt cgactattgt
     2881 ctcctacgga tgatagggga gtactctttt ctactataaa gtactttaag ctatcggtta
     2941 atcaggctgt gggataaaat agatcaatca tctcccaagc gcagcatgcg ccctggcaag
     3001 atgcctggca gaaagggcgc caaccagcga tatctcacct gcaagaactg cggcagcgat
     3061 tatttcggca aatttttttg cattcgtacc agggggatct cccgcgcctg caacccccat
     3121 catatccagg cattcaagct gcgtggctat tcgtgtccct ccacccactg ttcccacatt
     3181 caatgaagga agcgttaccg aacagtatag gccttcatct gtaagttcca ttgtggttat
     3241 cgcattactt gcttcaacaa catgtgcggc atcctggccg catgctatga acatggctgc
     3301 agcgatattt gcagcatggg cattaaaacc cagcgaccct gcgcgggctg aacccaaaag
     3361 gttcttcctg taatttactt ccgccatttt ttcaggtgtg gtttttagta tgtcagttat
     3421 cgtttcattt accaggaaca catctgcaga aacagttttt cccctgccga ggatggcatt
     3481 tatggcggca ggtttcttgt ccgaacacat attgccggat aaagcaacaa gctcaacgtc
     3541 attttctgat aatatgatgt caacggcggc atctgtggca atagttacca tattcattcc
     3601 catggcatct ttggtatcat aggaaaatcg aaggaacaca tttctacctg ctgtaaatgc
     3661 atttatagag agcaattccc caaaacgtgt cgtactttgc gctttttgtt tcagcatcac
     3721 aaagttctca tcgacccaat cagcaagtct ttttgccccg attatatctt ttgtcctgaa
     3781 aaccggagcc cttgtcatct catctttgaa tatcctcaca tttgcaccac ctgaggcagt
     3841 gatcactgaa cttcccctgt ccgcgcttgc cacaagcgca ccttctgttg ttgcaagcgg
     3901 aatatagtaa tcacctctgg catattcacc tttaatgcga aggggtccag ccactcccag
     3961 ggggacctgt attgctccaa tcatgttctc aatattgcgt ttggtgacgt tttcaacatc
     4021 aagggaataa ttggaaatat gattgaatat cgttccggtc agttccgaaa cagcagttcg
     4081 cctcaactcg atcgaagcat ttggatcaag cctttcatca atgtcatgaa aactgagctc
     4141 tccccttttt actttttcaa gaagttcttt gttcataaaa tttcaaacaa tttctctata
     4201 gtttttctgc aggcatcaaa cccgccatcg agatatatcg cgccgatcac cgcttcaaat
     4261 gtctctgcca gcacatacgg ctctttgtcc gctctttgtt ttgtctcgcc attcccaaat
     4321 ttcaagtaag agcccagccc aagccgaaca ctgatcttcg caagcccttc ttgcctctca
     4381 aggctttttt tccggttggt tatttcccct cttgagttga cgccgttttt gatcaaaaga
     4441 tcgatcaaaa ctgttttaag gacagcgtcg ccaagagttc taaagatttc ctgatcttca
     4501 caataaatgt tttgctgttt ttgttccagc gcataagctt tgcgtgtcag ggcgcggttc
     4561 aagagatttt tatccttgaa tttataactc agtttctctt cgagactcat ataataatga
     4621 aaaaagaaat gaaaaataaa aataattgaa tttatgtgat tagctggact tttttatcct
     4681 tgcaaccgca agcctgatat cttcttcttt gactgttgtc ctctttgcat gttttgcaag
     4741 ggttatggct tcacgcgaaa tttctatacc gtattcctca aggacttttg caagctcaac
     4801 gccggcatct tctgctaccc tgtctgctcc tgcttttcgg attatgcgct ctaccggcgc
     4861 aagtggaagt attggctgga cttgatttga catttatttt ctccttttta aattaaaatc
     4921 ttgaatagat attattggta ataacgatat atatagtttt ttaaatcaat acagagtgat
     4981 ttacaatctg attttcactt cttcaggttt gtaccgaagc ttaacaacac ggcttctccc
     5041 gcgcgctccc ttacctgtaa aatcggcatc tatcagcttg accgagccaa gcttattaag
     5101 caattcataa aaacgcgtat atccaagagc ggtcttttcg tggaattttt tgtacaggtc
     5161 gccggaattt gattgaggga aatccgcgat caaaccaagg agtatttttt cttccttttt
     5221 tgatgtacgc atcatgtaag aaagctgca
//
LOCUS       DATGBN010000353         9538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_493585, whole genome shotgun sequence.
ACCESSION   DATGBN010000353 DATGBN010000000
VERSION     DATGBN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493585"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VKL21_08715"
     CDS             <1..628
                     /locus_tag="VKL21_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HMB45890.1"
                     /translation="LAGRPLMEWILNRVRKAKSVDEIVLATSISSENDVLEKIAGNMG
                     VHTIRGDENDVLSRFETAAKECSADLIVRICADNPFIDPSEIDNLVNFYFKNRPEYAF
                     NHLNRLSNNYADGFGAEIFTRGVLEDMNRLCVEQRHREHVTMYLWDNPYAYRMQTFPA
                     PEKIAFPDLRFDVDTPEDLDYLQFIAEKVGMDGTAAEFIEAEMKRRNL"
     gene            625..1398
                     /locus_tag="VKL21_08720"
     CDS             625..1398
                     /locus_tag="VKL21_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HMB45891.1"
                     /translation="MNSNKTKQDIKIVATIEARMTSTRLPGKVLKPILGKPMLELLIE
                     RLQSAKNINEIVVATTINDTDNPIIDLCNQLNVKYFRGNEEDVLKRVLDAAKSVSADL
                     IVEITGDCPLIDPTIVDECIQLFLDGEYDYVSNSIEPSFPDGLDVKIFSVSVLEEVNN
                     LTDDPVDHEHVSIYIYEHPERYCLKNLKANNEMFWPELGITLDTQEDYELINIIFEEL
                     YPVNPNFSALDIVNLIRGRPELLDINKDIKRKDPHAEKI"
     gene            1382..2362
                     /locus_tag="VKL21_08725"
     CDS             1382..2362
                     /locus_tag="VKL21_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMB45892.1"
                     /translation="MQKKYNVAIIGCGNIAALYDEPQQYDRVLTHAHAYSLEPRVNLC
                     AMVDVQRHMADDAAKKWGGKPYYDAKDMLENENIDIISICVPDRYHENILDLCLSYKP
                     KAVLCEKPLTLDMTSAERIVDEYANAGIYLSVNFSRRFDTSIINLGKAIAQGTYGNVL
                     NAVGIYTKGILHNGSHLIDIFRFLFGDVIKTIPLSARNDWNDNDLTLDAYLEFYNGAK
                     AHLVGADEGQYSIFDINILCEKGRFQLSQFGLRMTEFHVRTDPVYPGCKDLNDGKCNK
                     TGLNTAMLYAISNIIDTIEGKQTLICSGVDAVITQKICLELLRKYHMSGA"
     gene            2367..3671
                     /locus_tag="VKL21_08730"
     CDS             2367..3671
                     /locus_tag="VKL21_08730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMB45893.1"
                     /translation="MEKLSILGGKPVRTKLFPAYKVIGEEEKAAVQKVMDTGILSRFL
                     GCWDQDFYGGPEVQEFEKEWADYFGVKHAIAVNSCTSGLYCAVGASGIGPGDEVIVSP
                     YTMSASATSPIIFNAVPVFADIEPDYFCLDPESVESKITERTKAIIVVDIFGQPYDAK
                     AINEIAEKHNLIVIEDCAQAPHARFKGRWAGTLGDIGVYSLNYHKHIHTGEGGVVVTN
                     NDELADRVRLIRNHAEAVVGDKGTSNLINMVGFNFRMNEIEAAIGRTQLKKLEKLADD
                     RIANAEYLAKELSSMDGIIAPGLREGCTHVYYIQCFKYYEDIVGVPRDRFIDAVKAEL
                     PATELREAEGVQLSAGYVKPLYLEPMYQNLIAYDSKGCPFKCPMYAGVPKYDRGLCPV
                     AERMHYKELFGHEFMRPPATKKDLDDVIRAFEKVMRHRKELT"
     gene            3795..4856
                     /locus_tag="VKL21_08735"
     CDS             3795..4856
                     /locus_tag="VKL21_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45894.1"
                     /translation="MGAAALLSKQWPEIKEIDDSTTIDKILNIWQRNKPKILLSGAGA
                     YNLLEQTVRLAAKEVNIPCVAVLDYWANYQERFRRREGKEWVYSIPDRICVLDEIVKN
                     EMLAERFSPETIVATGQPYFEYISNWINTLSPKDINLFRNRFMKDEKTLLIGFCSEPI
                     AEDLEMMHNDEIGYTQYTILSNVAPLLKRLAESRSIGIHLVVRPHPREENRNLKEILA
                     QMQSSSTFTWEVSKIGSSLEFALSCDLLIGMTSMALIEAIIMSCNVLSIQLNLNKKDV
                     FFGTTRGCCPSVYTDKELSNWLQNWLIGQKNNLRKFEPRFNTMLYKSGASQHVITIME
                     EMIEKSSLSSQSSQVTETI"
     gene            4853..5716
                     /locus_tag="VKL21_08740"
     CDS             4853..5716
                     /locus_tag="VKL21_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45895.1"
                     /translation="MKKNNYSLNDLPHFSPWPARLLGIEPCEQRYKTPEEITREYNYE
                     KWGTLLNRVIGSKEVSIEDVERWVLEDTPVSLCSVEDEFELLSTIEASRRHAELMENI
                     IKSYLPATALVELGAGYGNIILGLAKKEPFKKMSLIAGEYTASGVELITQLAQIQKIH
                     IQAEHCDFFSDSVLDFTIPEDALIYTSFAAHYVPKLSNNFIRSLSEFHPKAVIHAEPC
                     YEHFNRLTLLGLLRRRYMEVNDYNTNLVTLLHDQQDKGLIKILEERPAVFGMNPLLPA
                     SLLVWTPIFKS"
     gene            5739..7565
                     /locus_tag="VKL21_08745"
     CDS             5739..7565
                     /locus_tag="VKL21_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45896.1"
                     /translation="MSKNKKNLLIIQNISNDILNNLDHENFDFLCIDTYGMLKLKKKR
                     IPFLSFYDFYDREEFLKFLKDKLNTLKEIFRRLDLKYSEILDYPNAFTGNYYLFMVYF
                     ADYIFISEVASRLKNEYQNISIYTQYKKEDLEKYKTDIMINIFEPFSIVDNRKMLIIS
                     LLGENLKTSLIYSKPENKAETTNKYNTYISILCNTNPSILWSNLLDRINDKISFEKNK
                     KTKMVFSIDLSNEPKYLCPYMDEYDFVNPLHVIKKKGLEKKNIPDFLETESYSIKSLT
                     TLKSDTFFNILRIYQRDVMSFIPSLIERMNERISHSRPACVFFSHGQISTLENIVAFL
                     SNKHKIPVFNFQHGNDPLFFDRLEFYRYIELVENIEKTLIVTSKKQYDLLNESIPANT
                     KIFHGGSCKLFDYFHKLPKKSVNNKKILIITGMYPAAVYKDLAKMETDVEIFDKHNLI
                     FEILKKHDINFDIKIFPTVKNLNYFKEISQLYKINHINIMAQPNVELLLPNYDMIIME
                     YIGSALTPLLMTLKVPVIYYINKDFIDNNGLDDFCKRFYLVTNGNEFESIIKQFKEGK
                     LQSKYCEESILKFCFPSLTTNPAQEIAKYIKSQIFDSDKKYN"
     gene            7540..9045
                     /locus_tag="VKL21_08750"
     CDS             7540..9045
                     /locus_tag="VKL21_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HMB45897.1"
                     /translation="MILIKNIINQFIKGRKISNIIKIGAVQRQSIITLFWQIAFTAIG
                     FISTMYFAHKVGADILGAYFLFTAYQGIFSMVTDGGFGGAAVKRISEGYEQNAYFSAY
                     FVIRLVFTVLVIVALLLFREYFVDINESGMIIWLILLLIVSLLAGSISSGVVGIGKVG
                     ISATCAAIGNISRVIIQVVAIYLGYEVVGLVGGVLVGLLITAIIEFHFLDLRFVRFRR
                     THIKSLSVFSFWLFLTSGGVLVFSQADTIMIGYFMNNADVGIYRVALQFTMVATFTTY
                     ALRGTLFPKVSHWGKSGEVGLVEESLSRAISYSLILAVPVFMGGMLLGDRLLYFFYGE
                     DFASGYLALVALLAVQVVNVFQYFFTMYLDALDHPKESFKVTAVGVVSNIVLNIVLIP
                     VMGIDGAAIATLATMILNAMLAKNALSRLMTIRVERRSLLNIMTAAVVMGVLIGVYRL
                     LVPLSNVWITLLAVVLGGMAYVALVLKLDRRIMDELRGIVEGMGVLWPRWL"
     gene            9145..>9538
                     /locus_tag="VKL21_08755"
     CDS             9145..>9538
                     /locus_tag="VKL21_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HMB45898.1"
                     /translation="MKNPLKTRLQNNQLTIGSWITLANNNIPEIMAQSGFEWLTIDIE
                     HSVITLDVVQQMIQVIEGKGVVPLVRVGENNPNLIKRVMDAGAYGVIVPMVNTRDQAE
                     AAVKAVKYPPAGTRGVGLARAQGYGFDFD"
BASE COUNT         3127 a         1550 c         2018 g         2843 t
ORIGIN      
        1 acttgcaggc agacccctga tggagtggat acttaatcgt gtgcgcaaag caaagtcagt
       61 agatgaaatc gtgttggcaa catctatttc aagtgaaaat gatgttttgg aaaaaattgc
      121 cgggaatatg ggtgtccata caatccgcgg agatgaaaat gatgtgcttt cacggtttga
      181 aactgctgcc aaagagtgtt cagcggattt aatcgtcagg atttgtgcag acaatccttt
      241 tatagatcca tccgaaatcg acaatctggt aaatttctat ttcaagaatc gtcccgaata
      301 tgctttcaat cacctgaatc gtttgtccaa taattatgct gatggttttg gggcagagat
      361 ttttactcgt ggtgtgcttg aagatatgaa ccggctctgc gtggaacaac gtcatcgtga
      421 gcatgtaacc atgtatctat gggacaaccc ctatgcatac cgaatgcaga catttccggc
      481 accagagaaa atagcctttc ctgatctaag atttgatgtg gatactccgg aagacctgga
      541 ttatttgcag tttattgcgg aaaaggtggg tatggatggg acagctgctg agtttattga
      601 agctgaaatg aaacgcagga atttatgaat tcaaacaaaa caaaacaaga cataaagatc
      661 gttgccacaa tagaagccag aatgacttct acaaggctac ctggaaaagt attgaaacca
      721 attctgggga agcccatgct tgaattgttg atagagcgtc tgcaaagtgc aaaaaatata
      781 aatgaaatcg ttgttgcaac cacaataaat gataccgata atcccataat cgatctttgt
      841 aaccagttaa acgtgaaata tttcagggga aatgaagaag atgtattaaa acgtgttctt
      901 gatgcggcaa aaagtgtatc ggcagattta attgtcgaaa taacaggaga ttgtcccttg
      961 atagacccga ctattgtgga tgaatgtata caactctttt tagatgggga atatgattat
     1021 gtttcaaatt caatcgaacc ttcatttccc gatgggcttg atgtaaaaat attttcagta
     1081 tcggtgttag aggaagtcaa taatcttact gatgatccag ttgaccatga acacgtttcg
     1141 atatatattt atgaacaccc ggaacgatat tgtctcaaaa atctgaaagc aaacaatgaa
     1201 atgttctggc ctgaattggg gattactctg gacactcaag aagattatga attaataaat
     1261 atcatttttg aagaacttta tccggtaaat cctaactttt cagctttaga tattgtaaat
     1321 cttattcgag gcaggccaga actattagat ataaataagg atatcaaaag gaaagatcct
     1381 catgcagaaa aaatataatg ttgcaataat cggatgcggc aatatagcag ctttgtatga
     1441 cgaaccacaa caatatgaca gggttcttac gcatgcccat gcttattccc ttgaaccaag
     1501 agtaaatctt tgtgcgatgg ttgatgtcca gagacatatg gcagatgatg ccgcaaaaaa
     1561 atggggtgga aaaccatatt atgatgcaaa ggatatgctt gagaatgaaa atattgatat
     1621 tatcagcata tgtgtgcctg atagatatca tgaaaacata ctggatttat gcttatcata
     1681 taaaccaaag gcagttttat gcgaaaagcc cttaacttta gatatgactt cagcagagcg
     1741 aatagttgat gaatatgcaa atgcaggaat ttatctatct gtaaattttt caaggcgatt
     1801 tgacacatct ataattaatc ttggaaaagc aattgcacag ggaacatatg gtaatgttct
     1861 taatgcagtt ggcatatata caaaagggat tctacacaat ggttcacatc tgatagacat
     1921 attcaggttt ttatttgggg atgtaattaa aactattccg ttatctgcca gaaacgactg
     1981 gaacgataat gatctaacat tggacgctta tcttgagttt tataatggag ctaaagcaca
     2041 tttggtagga gctgatgagg ggcagtatag tatatttgat attaatatac tatgtgaaaa
     2101 aggccgtttc caattaagtc aatttggact caggatgact gaatttcatg tccgaactga
     2161 ccctgtttat cctgggtgca aagacttaaa tgatggaaaa tgcaataaaa caggattaaa
     2221 cacagctatg ctttatgcaa tttccaatat aatcgataca atagagggaa aacaaacatt
     2281 aatatgttca ggtgtagatg ctgttattac tcaaaaaata tgccttgaac ttttaagaaa
     2341 ataccatatg tctggagcat aatttcatgg aaaaattatc tattcttgga ggaaagccgg
     2401 tcaggacaaa actttttccg gcatacaaag tcataggaga agaagagaag gctgcagtgc
     2461 aaaaagtaat ggataccggc atattgtcca gattcctggg atgctgggac caggatttct
     2521 atggcggtcc tgaagtccag gaattcgaaa aggagtgggc tgactatttc ggagtgaaac
     2581 atgcgatcgc tgtgaattcc tgtacatcag gattatattg cgcagttggt gccagcggta
     2641 taggtccagg ggatgaagta atagtgtctc cgtacaccat gtctgcatct gcaaccagtc
     2701 ctattatttt caatgctgta cctgtatttg ctgatataga acctgattat ttctgtcttg
     2761 atcctgaaag tgtagaaagc aaaataaccg aacggacaaa agcaataatt gtagttgata
     2821 tttttgggca accttatgat gccaaagcga tcaatgagat cgcagagaaa cataatctta
     2881 tagtcattga ggattgtgca caggcacctc atgccagatt taaagggcga tgggctggaa
     2941 cccttggaga tatcggagtt tatagtctga attaccacaa acatatccat acaggagaag
     3001 ggggcgtagt agttaccaat aatgatgaac tggcagatag agtccgtttg atcagaaatc
     3061 atgcagaagc agttgttggg gacaaaggta catccaatct aatcaatatg gtgggtttca
     3121 attttcgcat gaatgaaata gaagctgcta ttggcagaac acaacttaaa aaacttgaaa
     3181 aacttgcgga tgatcgaatc gccaatgcag aatacctggc aaaagaactg agttctatgg
     3241 atgggatcat tgctccgggc ttaagggagg gttgtactca tgtttattac atacagtgct
     3301 tcaaatatta tgaagatatt gtgggtgttc cccgcgatag gttcattgat gccgttaaag
     3361 cggaactacc ggcaacagaa ttacgcgagg ctgaaggtgt gcagttatca gcaggttatg
     3421 taaaaccact ttatttggag ccgatgtacc agaatctgat agcttatgat tcaaaaggat
     3481 gccctttcaa atgtcccatg tatgctggag tccctaaata tgatagagga ttatgtcctg
     3541 tggctgaaag gatgcactat aaggaattgt tcgggcatga atttatgaga cctcctgcta
     3601 caaaaaaaga tttagatgac gtaatcagag cctttgaaaa agtcatgcgc catagaaaag
     3661 aattaacata agcctgaaaa tatatgacca tggatatttt tgttgttgcc agcgatcccg
     3721 gatgcgcaaa tgcgctgtca tctgtcatag cgacatgctg caaacaaggt cataatatca
     3781 atggtgtagt ttcagtgggt gcggcagctt tacttagtaa gcaatggcca gaaataaagg
     3841 aaattgatga ttctactact atcgataaaa tactgaatat atggcaaagg aacaaaccca
     3901 aaatcctatt aagtggagcg ggtgcatata atttattgga acaaacagtt cgtcttgctg
     3961 ctaaagaggt aaatattcct tgtgtagcag tcctcgatta ctgggctaat tatcaagaac
     4021 gatttcgacg gcgggaagga aaagaatggg tttattcaat acctgatcga atatgtgttc
     4081 ttgatgaaat tgtgaaaaat gagatgttag cggagagatt ttctccagaa accattgtgg
     4141 caacaggcca accgtatttt gaatatatct ctaactggat aaatacatta tccccaaaag
     4201 atattaatct ttttcgtaat cgatttatga aagatgaaaa aaccctattg atcggatttt
     4261 gttctgaacc aatcgccgaa gatttagaaa tgatgcataa tgacgaaata gggtatacgc
     4321 aatatactat tttatcaaat gttgcacccc tattgaaaag gttggcagaa tcgcgtagta
     4381 ttgggataca tctggtagtg cgtcctcacc ccagggaaga aaatagaaat ctcaaagaga
     4441 ttttggcaca gatgcaatca tcatcgacat tcacatggga ggtttccaaa attggctcat
     4501 ctctggagtt tgctttatca tgtgacttgc ttatagggat gacttccatg gcgctgatcg
     4561 aggcaatcat tatgtcatgt aatgtattga gtatccaatt gaacctgaat aaaaaagatg
     4621 tattttttgg aaccacacga ggatgttgcc cgagtgttta tactgataaa gagttgtcta
     4681 attggctgca aaattggctc atcggtcaaa aaaacaactt gcggaaattt gagccgcgtt
     4741 ttaataccat gctatataaa agcggagcca gccagcatgt aataacgatt atggaagaga
     4801 tgatagaaaa aagctccctg tcaagtcaga gcagtcaggt aacagaaacg atatgaaaaa
     4861 aaataattat tccttaaatg atttgccgca tttctcacca tggccagcgc gcttgcttgg
     4921 aattgaaccc tgtgaacaaa gatacaagac accagaagaa ataacccgag agtataatta
     4981 cgagaaatgg gggacgttat tgaatagagt gatcgggtca aaagaagttt caatagaaga
     5041 cgttgaaaga tgggtattgg aggatacccc ggtatctctg tgtagcgttg aagatgaatt
     5101 cgaattgctg tcaacaatcg aagcgagccg aaggcatgct gaacttatgg agaatataat
     5161 aaaatcatac ctccctgcga ccgcacttgt tgaattaggg gctggttatg gtaatattat
     5221 tcttggattg gcaaaaaaag agccatttaa aaaaatgagc ctgatagctg gagaatatac
     5281 tgccagcggt gttgaattaa ttacacaact ggctcagatt caaaaaattc acatccaggc
     5341 agaacattgc gatttctttt ctgattcggt tcttgatttc actattccgg aagatgctct
     5401 catttataca tcttttgcgg ctcattatgt gcccaagtta tcaaataact ttattagatc
     5461 tctttcagaa tttcatccaa aagcagttat ccatgcagag ccctgttatg aacattttaa
     5521 tcgcttgacc ttacttggtc tcttgaggcg ccgatatatg gaagtgaatg attataatac
     5581 caaccttgtc acattacttc atgatcaaca agataaaggt cttattaaaa tcctggaaga
     5641 aaggccagca gtatttggaa tgaatccatt gcttccggca tcgttactgg tttggacccc
     5701 aatatttaag tcctaaatat agtatataat attctgatat gtctaaaaat aaaaaaaatt
     5761 tgcttattat tcaaaatatt agtaatgata tattgaataa tttagatcat gaaaattttg
     5821 atttcctttg cattgatact tatgggatgc taaagttaaa aaagaaaaga attccatttc
     5881 tatcatttta tgatttttat gatagagaag aatttctaaa atttttaaaa gataaattga
     5941 atactttaaa ggaaatattc cggaggctgg atttaaaata ttctgagatt ctcgattatc
     6001 caaatgcatt tacaggtaat tattatttat tcatggtata ctttgcagat tatatattta
     6061 tttcagaggt agcatccagg ttaaaaaacg aatatcaaaa tatatcaatt tatactcagt
     6121 ataaaaagga agatctggaa aaatacaaaa ctgacatcat gataaatatt ttcgaaccgt
     6181 tcagtattgt agataacaga aagatgttga ttatttcatt attaggtgaa aatttaaaaa
     6241 caagtttgat atattctaaa cctgaaaata aggccgaaac tacaaataaa tataatacat
     6301 atatatctat tttatgtaat acaaacccct caatattgtg gagcaattta ttagatagaa
     6361 ttaacgacaa gatttcattt gaaaaaaata aaaaaacaaa gatggttttt tcaattgatt
     6421 tatccaatga accaaaatat ctgtgtcctt atatggatga atacgatttt gtaaatcctt
     6481 tgcatgttat taagaaaaaa gggcttgaaa aaaaaaatat tcctgatttt ttagaaactg
     6541 aaagttattc gatcaaaagc ttaacaaccc tgaaaagcga cacgtttttt aatattttga
     6601 gaatttacca acgtgatgtt atgtccttta taccttcact tattgagaga atgaatgaaa
     6661 gaataagtca ctcaagacca gcatgtgttt ttttttcaca tggccaaata agtactttgg
     6721 agaatattgt ggcttttcta tcaaacaagc ataaaatccc ggtatttaat tttcagcatg
     6781 gtaatgaccc attatttttt gatagacttg aattttacag gtatattgaa cttgtggaaa
     6841 atattgaaaa aaccctgatt gtaacctcaa aaaaacaata tgatcttttg aatgaatcaa
     6901 tacctgccaa tacaaaaata tttcatggag gaagttgtaa gttgtttgat tatttccaca
     6961 aattgccgaa aaaaagtgta aataataaaa aaattttaat tataacagga atgtatccag
     7021 ctgctgtata caaggatctg gctaaaatgg aaacagatgt tgaaattttt gataaacaca
     7081 atttaatttt tgaaatatta aaaaaacatg atattaactt tgacatcaaa atctttccga
     7141 ctgtgaagaa tttgaattat tttaaagaaa tatcacaatt atacaagata aatcatatta
     7201 atataatggc acaaccaaat gtcgagctgt tgcttccaaa ttatgatatg attattatgg
     7261 aatacatagg ctctgcttta acaccccttt taatgacttt aaaagtgcca gtaatctatt
     7321 atattaataa agattttata gataataacg gtcttgatga tttctgcaag aggttttatc
     7381 tggtaacaaa tggaaatgag tttgaatcaa taataaagca gttcaaagaa ggtaaattac
     7441 aatcaaagta ttgtgaagaa tcaattctta aattttgctt tccttcatta actaccaatc
     7501 cagcacaaga aattgcaaag tatatcaaaa gtcaaatttt tgattctgat aaaaaatata
     7561 attaatcaat ttataaaggg gagaaaaatt tcaaacataa tcaaaattgg cgcggttcag
     7621 aggcaaagca taataacatt attctggcag attgcattca cagctatcgg cttcataagt
     7681 acaatgtatt ttgcccataa agtaggtgcg gatatcctgg gtgcatattt cctgtttaca
     7741 gcgtaccaag gcatttttag tatggttaca gatggaggat ttggcggagc agctgtcaaa
     7801 cgtatcagtg aaggttacga gcaaaatgcc tattttagtg catattttgt tattcgtttg
     7861 gtattcacgg tattggtcat agttgctttg cttctattca gggagtattt tgttgatatt
     7921 aatgaatcag gaatgatcat atggttgata ttgcttttaa tcgtatcatt gcttgcgggc
     7981 tcaatctcaa gtggagtggt aggtattggc aaggtcggaa taagtgccac atgcgcagct
     8041 attggaaata tttcacgtgt aatcattcag gtcgtagcaa tttatttagg gtatgaggtt
     8101 gtgggactgg tcggtggagt tctggtagga ttacttatta ctgccataat tgaattccac
     8161 tttcttgatc tgcgattcgt gcgctttaga cggactcata tcaagagtct ttcagtattc
     8221 tcattctggt tgtttttaac atctggtgga gtgctggtct tttcacaggc agacactatc
     8281 atgattggat atttcatgaa taacgcagat gtaggcatct atagggtagc gctccagttc
     8341 actatggtcg caacttttac cacatatgca ctgcgtggaa cactatttcc caaagtcagc
     8401 cattggggta agagcggtga ggtcgggctg gtggaagagt ctctgtcgcg tgcgatcagc
     8461 tactcgttga ttttggcagt tcctgtattc atgggcggca tgctgctggg ggacagacta
     8521 ctatacttct tctacggaga agactttgcg agtggttatc tcgcactggt agcactactt
     8581 gctgtgcagg tggtaaatgt gttccagtac ttcttcacaa tgtatctgga tgccctggat
     8641 catccaaaag aatcatttaa ggtaactgct gtaggtgttg tttcaaacat cgttctgaac
     8701 atcgtgctga tcccagtcat gggaatcgac ggcgcggcga ttgcgacact ggcaacaatg
     8761 atattgaatg ctatgcttgc taaaaatgcg ctgtcaagat tgatgacaat aagagtggag
     8821 cgtcgcagtc tgttgaatat catgaccgca gcggttgtga tgggggtgct tattggcgtg
     8881 tatcgtttgc ttgtgccgct ttccaatgtt tggatcacat tgctggcggt tgttttaggt
     8941 gggatggcat atgttgctct cgtgttgaaa ctagatcgga ggataatgga tgaattgcgg
     9001 gggattgttg aaggaatggg agtgttatgg ccgaggtggt tgtaggttta tatggaatta
     9061 acaatatatc agtagcaaaa tagcaattgt atttaccatg tcggcaataa catttaatat
     9121 aatcaacaaa gtgggtgtaa aacgatgaaa aatccattga aaacaagact gcagaataac
     9181 caactaacaa taggtagctg gataaccctt gcgaacaata acatcccgga aataatggca
     9241 caatcaggtt ttgagtggct aacgatcgat atagaacaca gcgtgataac tctcgatgtt
     9301 gtccagcaga tgatccaggt gatcgaagga aaaggagttg tccctcttgt cagggttggc
     9361 gagaacaacc ctaacctgat aaagcgcgtg atggacgcgg gcgcgtatgg tgtcatcgtc
     9421 cccatggtca ataccaggga tcaggctgaa gcggcggtaa aagctgtaaa atatccccct
     9481 gccggcacgc gcggtgtggg actggcaaga gcgcagggat atggttttga ttttgatg
//
LOCUS       DATGBN010000354         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_183391, whole genome shotgun sequence.
ACCESSION   DATGBN010000354 DATGBN010000000
VERSION     DATGBN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183391"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1649
                     /locus_tag="VKL21_08760"
     CDS             <1..>1649
                     /locus_tag="VKL21_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein domain-containing protein"
                     /protein_id="HMB45899.1"
                     /translation="DWGNATLIQNNRTGLEKFDLEQNKFTVYEGESVSLGDGYFLKLV
                     EIANARDVALIQILKNDIMVDEVSLLIGTPYEYDREVTIDNSTLKIPDITINITSMFK
                     GSRGLIQFEGYRSRKIHTDKESNNSACFSCHQYRYSDKKERYVIIDKENQENYDDIYY
                     TKVFVDMKPGNKSKIYLDNDDYVFDQLENFSEKYISDPYKKKYLKEGETWNISDNYSL
                     KLNEASTDSEVALLTLTINGIIVESGPISKGHELKYTPAIEYKPYTQTNVTVFIANIT
                     SIFQAKTNFILLEDVSAISPDIMKINSNLTLFGYNSSWFQINDTFATGRIPPDLHSPN
                     QYDNTRNWADCVRCHDSSNHLRIENFDAISSKLGKHSGLNRNASNRTFLSDPIDKACW
                     ACHTGGTEPQMHSPTYITPRDCRSCHTSQKEPFYGATYVGDELHGNLSGCESCHIIGI
                     HTIQRFQVTPVIKEAVISKNKVNRGDAVNITAKAVAGFGMRIKAVEYFLDVKGNPGNG
                     TPFNSSDQKFNSQIKEAAATLNTTNISTGAHIVYIHAMERN"
BASE COUNT          576 a          320 c          327 g          426 t
ORIGIN      
        1 gattggggga atgcaacatt aatacaaaat aacaggacag gtcttgaaaa gtttgacctg
       61 gaacagaaca aattcacagt ttatgaagga gaaagtgtct ctctcggcga cgggtatttt
      121 ctgaaacttg tagaaatagc caatgccagg gatgttgcgc ttatacagat cttaaaaaat
      181 gatataatgg tggatgaggt ctcactttta atcggtactc catatgaata tgaccgggaa
      241 gtcaccatcg ataattcaac attaaagata ccggacatta ccataaacat aacctcaatg
      301 ttcaaaggca gccgaggtct tatccagttc gaaggataca ggtcaagaaa gatacatacc
      361 gataaagaaa gcaataacag tgcatgcttt tcctgccacc agtaccggta ttctgacaaa
      421 aaagaacgct atgttattat tgataaagag aatcaggaaa attacgatga catatattac
      481 actaaagtat tcgttgatat gaaacctgga aacaagagta aaatatacct tgataatgac
      541 gattatgtat tcgatcagct tgagaatttt tcggagaaat acatttcaga tccatataaa
      601 aaaaagtatc tgaaagaagg agagacctgg aacatatctg acaattattc attaaaactc
      661 aatgaagcca gtacagatag cgaagttgct ttgttgacac tgacgatcaa cggcattatt
      721 gttgagagcg gtcctataag caaaggtcat gaattaaaat atactccagc cattgagtat
      781 aaaccatata ctcagaccaa cgtaacagtt ttcattgcga atataaccag tattttccag
      841 gcaaaaacga attttatatt acttgaggat gtatctgcaa tatctcctga tataatgaaa
      901 atcaactcaa atctcaccct ttttgggtac aatagcagct ggttccagat caatgataca
      961 tttgcaacag gcaggatccc gcctgacctt cattccccga accagtatga taacacgagg
     1021 aattgggcag actgcgtgag atgccatgat tcttcaaatc atttgcgaat tgaaaacttt
     1081 gatgcgatat catcaaaact cggaaaacac tctggattga atcgcaatgc atccaacagg
     1141 acgttcttat cagatccaat cgataaagca tgctgggcat gccatactgg cgggacggag
     1201 ccccagatgc attccccgac atacattaca cccagggatt gtaggtcatg ccatacttct
     1261 caaaaggagc cgttctacgg tgcgacatac gttggcgatg agcttcacgg caacctatcc
     1321 ggttgcgaat cctgccacat tataggaata catactatac aaagatttca ggtaactcca
     1381 gtgataaaag aagccgtaat atctaaaaat aaagtgaaca gaggagatgc agttaatatt
     1441 actgctaaag cagttgcagg gtttggtatg aggataaaag ctgttgaata tttccttgac
     1501 gtgaaaggaa atcccggaaa tggaactcct tttaattctt cggaccaaaa gttcaattca
     1561 cagatcaaag aagctgcggc aactctcaat accaccaata tttcaaccgg ggcgcatatt
     1621 gtttatatcc atgcaatgga aagaaataa
//
LOCUS       DATGBN010000355         6042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_27802, whole genome shotgun sequence.
ACCESSION   DATGBN010000355 DATGBN010000000
VERSION     DATGBN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27802"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..374
                     /locus_tag="VKL21_08765"
     CDS             192..374
                     /locus_tag="VKL21_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB45900.1"
                     /translation="MKNAGFIIVLGCKKCGKCDGVCETGALAHVEGIARIDHEKCNRC
                     MRCVKVCPNKALVYLD"
     gene            458..958
                     /locus_tag="VKL21_08770"
     CDS             458..958
                     /locus_tag="VKL21_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HMB45901.1"
                     /translation="MELINAVVGIIQLVIAIILAVVALYIGFSVLGKITKGIDEEKEI
                     ARGNTAVGILVASVFIAIGIVVQSGVSGISVGISKALTPGLPYGDSIAIILISIVQLI
                     LGIVLAIVAIYLALNILDKLTKGIDEFAELKKGNVAVALEMAGVIITVAIIIQSGVMG
                     ITRALI"
     gene            1037..3619
                     /locus_tag="VKL21_08775"
     CDS             1037..3619
                     /locus_tag="VKL21_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HMB45902.1"
                     /translation="MNFQILDADYTYSDDKPVVRLYGRDDSGNSVCCLVPDFEPYFYA
                     KAASEMAGILQEKFKEHIKRIEPVQRFEPVGYFKNKTSMLKIILFDPKSVPVIRDDVR
                     NMVDDVYETDILFRNRYMVDTGLGGMGWAKSEPEGYPVRNEVLCDQNITSRYVEHLEI
                     LKNAPLRHLAFDIECLPLNGAMPTPENSPIVLISLAFAPDFEGKKTLVLIGKDARTEG
                     ENESCGSEEKMLCRFFKIIKDYDPDILVGYNSNSFDIPYIVDRIKTLNKKGARIEPIA
                     GRDGRTLYYRKIGNVTRVSVMGRIAVDVLPLLRREFSLKQYTLRNAAKELLACEKLEI
                     PFLEMESYWNDNGDKLSKFIEYARRDSELALLFLLKLRLIDKYIALARVSGTLLQDIL
                     DGGQTQMVENLMLREYMKNDRVLPARPTGEISNERYDEGEELAGAEVLPPKKGLLENI
                     VILDYKSLYPTIMMAHNLCYTTEIVGQRPDDVVVAPTGGVFVSRNIVKGIVPSILEDL
                     LNRRQETREKMKTANEEEKRVLDATQLALKILLNSFYGYSGYTRARLYSLTLASAVTS
                     FGRENILRTKDLIENQIKEIILKDGKALKKDENISGKHIGLSVVYGDTDSVFVNLLGD
                     GITFDEAQLVGNTIASIVTDSLVKPMELVFDSFARRAIFLAKKRYALLIQEKTAMGMK
                     EKIKVKGMETVRRDWCELTTKTIEKVLELVLIEGKVDDAVLLVKNTINSIKTIDASSP
                     LFEDLILTRQYTKKIESYRNRQPHITVIEKLQKRGIVSNVGDRIPFVIVAGNAIFVDR
                     AEDPEYAQKKNLPIDVDYYINKQILPPVERILSDFGVTREMLRGMGERKVQIETKQKQ
                     LFEF"
     gene            3631..4491
                     /locus_tag="VKL21_08780"
     CDS             3631..4491
                     /locus_tag="VKL21_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HMB45903.1"
                     /translation="MKTHPRCAPCLLSRVQFEAELSTKDLGLQKKVVLAGIEVLRKYM
                     VDGAPATHLSTKIHREAYRILGDVDPYSLKKKESNEEAKKLMPFVREFVDEKDSFRRA
                     VLVSIIGNSFDFGVLGFDADKAIAKAAMQEQITHGLDVDDSDKIKTMLNDVVYLADNC
                     GEIVFDMLLFEQIKKLGGNITLVMRGAPILNDVTMKEALELGIDRMVDRVLTTGSNAI
                     GLCLQEAPPELVEALGNASLVISKGMANYETMSEYDFRPIAYLLRTKCESVAEAMGLK
                     KNMNVARVED"
     gene            complement(4549..>6042)
                     /locus_tag="VKL21_08785"
     CDS             complement(4549..>6042)
                     /locus_tag="VKL21_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMB45904.1"
                     /translation="SDLKNGTLATVCGIYRSGLIDPDSVNPVLAIATEKDVYYSNETL
                     RVHIDFYSQIDGKAKLEVSGIRNDFDRPLVEKVQDIAFIKGINGFDFEFETPSCEECS
                     GLSPGEYVVNATVNLSEKSFETYKEITLARDNSTQNKTGISQINDSQINDTQAESTYS
                     VPGSNNTILVEYFYITGCQKCEQATPVVETIMKSYGEKVNFIKYNAKEEGRELAIKYS
                     IPGTPSIVIDKDRKNLISYENYNGDTSKLEAVLKGAIDNAYNSQKAPDVAQNEKIILS
                     VPSVFVVGFLAGFNPCLLAILAFIASVTLATTGKRRNVLLIVLMFSLGIFVTYLVVGI
                     GLLRILEEAPGLQAGIKNFLVVLIGILGLWHVYDANHLRTNTESSFYTPKAFIRLTES
                     VTKKVSLPASFFMGALFSLIKAPCVGAVYFLILDMVRSGEGTGYLYLAAYNFGVVLPV
                     LVLGGAIAFGLDPEKVEKFRKDKRSMMRLVTGVTLLIIAFLMYIGKI"
BASE COUNT         1833 a         1167 c         1385 g         1657 t
ORIGIN      
        1 tgggaccttt tgatacgagt actttgtaag aatctatatc gatattctta agcccgagga
       61 tacactttgc gatatcgtcg catttgaaat ctccccggat catttccgga attgatcttg
      121 gcataataat cctgtgtttt gcacaatttg tacaaaacct atctatacct ataagtttaa
      181 ataacttatg aatgaaaaat gcgggtttca taattgttct tgggtgtaag aaatgtggaa
      241 agtgtgacgg tgtatgcgaa acaggagctc tggcacatgt cgaaggtatt gccaggatag
      301 atcatgaaaa atgcaatcga tgtatgcgct gtgtcaaggt atgtccgaat aaggcccttg
      361 tgtatcttga ttgaatgttt tatattaaag ttaacacaat aattatatat taccctatta
      421 ccaatatagg attgcgctaa aagcgtgagg tgaataaatg gaactaataa acgcagtcgt
      481 aggaataata caattagtca ttgccattat acttgcagtc gtggcattgt atattggttt
      541 ttccgtattg ggaaagataa ctaaagggat tgacgaggaa aaggaaatcg ccaggggaaa
      601 tacggcagtt ggtattcttg tggcatcagt gttcattgcg ataggaatag tagtacagtc
      661 gggagtttca ggtatttcag tgggaatttc aaaggcttta acgcccggcc ttccatatgg
      721 tgatagtatt gcaattatcc tgatcagcat cgttcaactg atactgggga tagtgcttgc
      781 aatagtagca atatatcttg cattgaatat tctcgataaa ttgactaaag gaatcgatga
      841 attcgctgaa ctcaagaaag gaaatgttgc agtagctctt gaaatggcag gtgttataat
      901 cacagtcgcg attatcatcc agtcaggagt aatgggtatt acgcgtgcat tgatctaaag
      961 aacaattctt ctttttttca atatttctct tttttttaaa gaaacatata aacacattaa
     1021 atcaaatcta accataatga acttccagat tctcgatgcc gactacactt acagcgacga
     1081 taaacctgta gtaaggctct atggaaggga tgactccgga aatagcgtct gctgcctggt
     1141 tcctgatttt gaaccttatt tttatgcgaa agcagcgtct gaaatggcag ggattttaca
     1201 ggaaaagttt aaagagcata taaaaaggat cgagccggtt caaaggtttg aacctgtggg
     1261 ctatttcaag aacaaaacca gcatgctaaa gatcatcctc tttgatccga aaagcgtgcc
     1321 cgtcatccgt gatgatgtga ggaacatggt ggatgatgtt tatgagactg atatcctttt
     1381 ccgcaacagg tatatggtgg atacaggact tggcggtatg ggctgggcta aatcggaacc
     1441 ggaagggtat cctgtgagaa acgaggtctt atgcgaccag aatattacat caaggtacgt
     1501 agaacatctt gaaatcctga aaaacgcacc cttgcggcat cttgcatttg atatcgaatg
     1561 cctgccttta aatggcgcaa tgccaactcc tgaaaattca cccatcgtcc ttattagcct
     1621 tgcttttgct cccgattttg agggaaagaa aacacttgtg ctcattggca aggatgcaag
     1681 aacagaagga gaaaacgaga gctgcggcag cgaagaaaaa atgctgtgca ggttttttaa
     1741 aataataaaa gattacgatc ccgatatcct tgtgggctac aactcaaaca gttttgacat
     1801 tccttatatc gttgacagga tcaaaacact caataaaaaa ggagcacgca tagaaccgat
     1861 cgcaggcagg gacggcagga ccctctatta caggaaaata gggaatgtaa caagagtaag
     1921 cgtgatgggg cgcattgctg tagacgtgct tccgctcctt cggcgtgaat tttccctcaa
     1981 acaatacaca ttaagaaatg cagcaaagga attgctggcc tgcgaaaaac ttgaaatacc
     2041 attccttgag atggaatcat actggaatga caacggcgac aagctttcga aatttatcga
     2101 atatgcaagg cgcgactcgg aacttgcgct tcttttcctg ttgaaacttc ggcttattga
     2161 taaatacatc gctcttgcga gggtgagcgg aactcttttg caggatatcc tggatggcgg
     2221 gcagacccag atggtggaga acctgatgct tcgcgagtac atgaaaaatg atcgggtcct
     2281 tccggcgcgt cccacaggtg agatatcaaa tgagcgctat gatgaggggg aggaacttgc
     2341 gggtgcggaa gtactgccgc caaagaaggg tcttcttgaa aatattgtca tccttgatta
     2401 caaatcactt taccctacta taatgatggc gcataacctc tgttatacta ctgaaattgt
     2461 aggccagaga cccgatgatg tggtcgttgc gcctaccggg ggtgtttttg tttcaaggaa
     2521 tatagttaag ggcatcgtgc catctattct tgaagatctc ttgaaccgta gacaggagac
     2581 gcgcgaaaag atgaagaccg caaatgagga ggaaaaacgc gtacttgatg caacgcagct
     2641 tgctcttaag atcctgctta acagttttta tggctattca ggatatacca gggcacggct
     2701 ttacagtttg actcttgcaa gcgcagtaac gagcttcggg cgcgagaata tcctgcgaac
     2761 aaaagatttg atagagaacc agatcaagga aataatcctg aaggacggaa aagctcttaa
     2821 gaaagatgaa aacataagcg gaaagcatat cggattatcc gttgtttatg gagatacgga
     2881 cagcgttttt gtgaatcttc ttggtgacgg gatcactttt gatgaagcgc agctagtggg
     2941 aaatactatc gccagtattg ttacggattc tcttgttaag cccatggaac ttgtttttga
     3001 ctcttttgcc aggcgcgcta tatttcttgc caagaaacgg tatgccctgc ttatccagga
     3061 aaaaactgcc atgggaatga aagaaaagat aaaagtaaaa ggcatggaaa ccgtgcggcg
     3121 cgactggtgc gaacttacca caaagacgat cgagaaggtc cttgaacttg tgctgataga
     3181 aggaaaggtg gatgatgctg ttcttcttgt caagaataca ataaacagca ttaaaacaat
     3241 agatgcaagc agcccgcttt ttgaagacct tattttgaca aggcaatata ccaagaaaat
     3301 tgaaagttac aggaacaggc agcctcatat aactgtaata gaaaaacttc agaaaagagg
     3361 catagtttcg aatgtgggtg acaggatacc ttttgtgatc gtggcaggca atgcgatttt
     3421 tgttgaccgc gcagaggacc ctgaatatgc gcaaaagaaa aaccttccta tcgatgttga
     3481 ttattatatc aataaacaga tacttccgcc ggttgaacgt atattatctg atttcggagt
     3541 aacaagggaa atgttaaggg gaatggggga aagaaaagtg cagattgaaa caaaacaaaa
     3601 acaattattt gaattttagg agaaaaaatc ttgaagaccc atcccagatg tgcgccatgc
     3661 ttattatcaa gagtacagtt tgaggcagaa ctttccacaa aagacctggg attgcagaaa
     3721 aaagtggtgc ttgcaggcat agaagtgctt cgaaaatata tggttgacgg cgcgcctgcg
     3781 acccatcttt caactaagat acacagagaa gcatacagga tacttgggga tgttgatcca
     3841 tattccttga aaaaaaaaga gagcaatgaa gaggcaaaaa agctcatgcc ttttgtcagg
     3901 gaatttgtag atgaaaagga ttcattcagg cgcgcggtgc ttgtatcgat tatcggcaac
     3961 tcatttgatt tcggggttct tggatttgac gctgataaag caatagcaaa agccgcgatg
     4021 caggaacaga tcacacacgg acttgatgtg gatgattcgg ataagataaa aacgatgctc
     4081 aatgacgttg tctatcttgc tgataattgc ggcgagatcg tatttgacat gttgcttttc
     4141 gagcaaataa aaaagctggg gggtaatatc acacttgtta tgaggggcgc gcccatattg
     4201 aacgatgtca ccatgaaaga agcgctggaa cttggtattg acagaatggt cgacagggtg
     4261 cttaccaccg gttcaaatgc cattggtctg tgcctgcaag aggctccgcc tgaacttgtg
     4321 gaagccctgg ggaatgcatc gcttgttatc agcaagggaa tggcgaatta cgagacaatg
     4381 tccgagtatg atttcaggcc gatcgcatat cttttgagga caaaatgcga atcggtggct
     4441 gaagcgatgg gattgaagaa gaatatgaat gttgcaaggg tggaagattg atactcttct
     4501 gaaagcggaa gctttcttgc cccccattta atccgtttgt ataagaaact atatttttcc
     4561 tatatacatc aagaaagcaa tgatcagcag agtcactccg gtgacaagac gcatcattga
     4621 tcttttgtct ttcctgaatt tctcgacttt ttcaggatca agaccaaaag ctatcgcacc
     4681 gccaagaaca agaaccggaa gcacaacacc aaagttatag gccgcaagat aaagatagcc
     4741 tgtaccttcc ccgcttctta ccatgtcaag tataaggaaa taaaccgctc caacgcatgg
     4801 tgcttttata agcgagaata gggcgcccat aaagaacgat gcaggtagac tgactttctt
     4861 agtcacgctt tctgtgagac ggataaaagc tttcggggta tagaaagagg attccgtatt
     4921 tgttctgaga tgattcgcat catagacatg ccagagcccc aggattccga ttagaacgac
     4981 aaggaaattc ttgatgccag cctgcaatcc gggcgcttct tccaatatcc ttagaagtcc
     5041 tatccccact acaagatatg tcacaaaaat tcccaggctg aacatcagta cgataagaag
     5101 gacatttcgc cttttgcccg tagtcgcaag tgtcacggat gcaataaaag caaggatagc
     5161 gagaaggcat ggattaaaac ctgctagaaa tcccacaaca aaaacagatg gcacagagag
     5221 tattattttc tcattttgcg caacatcagg ggctttttgt gaattatagg cattatcaat
     5281 agcgcctttc aagaccgctt caagttttga tgtatcaccg ttgtaattct catatgatat
     5341 cagatttttg cggtctttat ctatgactat agaaggcgtt ccgggaatgc tatactttat
     5401 tgctaattcc cttccttctt ctttggcatt atattttatg aagtttacct tttcgccgta
     5461 ggatttcata atggtttcaa caacaggcgt agcctgttcg cacttctggc atcctgttat
     5521 atagaagtat tcgaccaata tcgtattatt cgatccggga acagaatatg tagactctgc
     5581 ctgtgtatca tttatctgtg aatcatttat ctgcgaaatc cccgttttat tctgcgtgga
     5641 attatcccgt gcaagcgtga tctccttata tgtttcgaag gatttttcac taagattcac
     5701 agtggcattg accacatatt caccaggtga gagaccagaa cattcctcac acgatggggt
     5761 ctcgaattca aaatcaaaac cattgatccc tttaataaaa gcaatatcct gtaccttctc
     5821 tactaaaggc ctgtcaaaat catttcttat gcctgatact tcaagcttcg cttttccgtc
     5881 tatctgggaa taaaaatcga tatggactct taatgtctca ttggaatagt aaacgtcttt
     5941 ttctgtagct attgccagta cagggtttac tgaatccgga tctataaggc cggacctata
     6001 tatcccgcat acggttgcaa gagttccgtt tttcaggtcc ga
//
LOCUS       DATGBN010000356         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_300209, whole genome shotgun sequence.
ACCESSION   DATGBN010000356 DATGBN010000000
VERSION     DATGBN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300209"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VKL21_08790"
     CDS             complement(<1..898)
                     /locus_tag="VKL21_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMB45905.1"
                     /translation="MKAIVYTKYGPPDVLHLEEVTKPAPKDNEILIKIYATSVNYGDI
                     IARNFREISPSKFNMPFLFWIFGKIAFGLRKPRINILGSEFAGEIESAGKDVKSFKKG
                     DQVFGYLGQSMGAYAEYLCMPENGCVAIKPANMTYEEATVVPYGAIMALNLLRKVNIQ
                     PGQKVLVNGASGGIGSAAVQLAKYFGAEVTGVCGTLRLELVKSLGADKVIDYTKEDFT
                     QSGETYDLIFDILGKSSFSSCKSSLKQNGRYLLASFKMKQLFQMLWTSMIGSKKVVCA
                     LAIDKQEDMIFIKELIEAGKIKS"
     gene            complement(895..1842)
                     /locus_tag="VKL21_08795"
     CDS             complement(895..1842)
                     /locus_tag="VKL21_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMB45906.1"
                     /translation="MKAIVCTKYGPPEVLQLKEVEKPTPKDNEVLIKIHATTVHIGGD
                     TKIRRLEPGLGFVKDLFFKPIMRMIIGFTGPRKKILGMELAGEIEAVGKDVTLFKRGD
                     PVFASTELMRFGAYAEYICLPEDGVLAIKPANMTYEEAAPVPNGGITALLILRKANIQ
                     SGQKVLIYGASGSVGTFAVQLAKNFGAEVTGVCSTANLEMVKSLGADKIIDYTASDFT
                     KNDETYDVIFDAVGKIAYSRVKKSLKKTGIYLNVLTSSDTLKLKTVDLVSLKELVEAG
                     KLKSVIDRTYPLEQIVEAHRYVEKGHKKGNVVITIEEHL"
     gene            complement(1867..2502)
                     /locus_tag="VKL21_08800"
     CDS             complement(1867..2502)
                     /locus_tag="VKL21_08800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB45907.1"
                     /translation="MNKSEKFWDRIAKYFDRVERKDEPTNIKIIEKTRNRLKISDNVL
                     DYGCGTGTAAIEIASSVKTVTGIDISSKMIEAAKGKTVELKVKNIDFAQTTIFDEKLK
                     KGSFDVILCFYLLHLVEDTPKVMQRINELLKPGGLIISATPCIKGTFLGILLSPLSKI
                     GLIPQITSFKITELEDLMTDGNFEIVETECLHKSGQQYFIAAKKKIENITT"
     gene            complement(2759..3931)
                     /locus_tag="VKL21_08805"
     CDS             complement(2759..3931)
                     /locus_tag="VKL21_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HMB45908.1"
                     /translation="MGSIHLGCSGWDYRDWADVFYKKTDESKLKAYSRIFNTAEINST
                     FYSYPAPGIVFGWAKHTPPDFKFAVKLNRLITHEKMLDLSKGVEDDLRRFCELMKPLK
                     ETEKLACILVQLPPGMKFKKDRIEAFLRILPLDMRFALEYRNETWLTDEAHDMLSSHN
                     VAAVAVDEPLLPPEIRRTSDIAYVRWHGRGKKMWYNYRYSKDELAQWLPRIKEMSHNV
                     EVYGYFNNHYHGYAPENCIDVLEMLGVATPEQKEARQHISDYRKSKVKEKIVATTLED
                     FLESEKEDVMTLLSEYIDALRLDRAKEIKDIEIIELSMEKIIADVRGYSVYVDLKRRF
                     ILHDCGDWRQTQREKRFCKHIGALMLALPEDAARSVLAGIKREKWEFSQYTGKGVL"
     gene            3971..4192
                     /locus_tag="VKL21_08810"
     CDS             3971..4192
                     /locus_tag="VKL21_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45909.1"
                     /translation="MLYIGAGGWAYFKVPGLDSLAAYSKAYDRIVGLRGERKPPASAV
                     GIAHPLVSKCLSPKKELSILHFLGIDRHK"
     gene            4225..>4759
                     /locus_tag="VKL21_08815"
     CDS             4225..>4759
                     /locus_tag="VKL21_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45910.1"
                     /translation="MKKPKSKTPSFITELPLKTTSADESTILVRLDAGRQLYHACLGE
                     GLERLALIQQSKEYQEIQKLPKTIDGKQNTERTKAFNDLNRKHGFTDYALQHYATGIR
                     NSWMEKHIHAHIAQKLATRAFKGVQKKAFGIAKNVRFKRKNQFDSLEGKNETGLIFKN
                     NALFWSGLEIPCIIDPKN"
BASE COUNT         1360 a         1072 c          800 g         1527 t
ORIGIN      
        1 ctgattttat ctttcccgcc tcaataagct ctttgatgaa aatcatatct tcttgtttat
       61 cgatcgccaa cgcacatact actttcttgc tgcctatcat tgaagtccat agcatttgaa
      121 aaagctgttt catcttaaag ctggctaaaa gataacgtcc gttttgtttt agtgagcttt
      181 tacagcttga aaacgaactc ttgcctaata tgtcaaaaat aagatcatag gtctcaccgc
      241 tttgggtaaa atcctctttt gtgtaatcaa tcaccttatc tgctcccaga gatttcacca
      301 attctaatct cagagtaccg cataccccgg tcacttctgc cccaaagtac ttggcaagct
      361 gtaccgctgc tgaacctatc ccgccgctgg ctccattgac caaaaccttt tgtcctggct
      421 ggatatttac ttttctgagc aaattcaacg ccattattgc cccataagga acaacggtcg
      481 cttcctcata agtcatattg gcaggtttta ttgctacaca cccattttca ggcatacaaa
      541 gatactcggc ataagcaccc atgctttggc cgagatatcc aaaaacctgg tcacctttct
      601 taaatgattt tacatctttg cctgctgatt caatttcccc ggcgaactca ctgcccagta
      661 tatttattct tggttttctg agaccaaacg ctattttccc gaatatccag aagaggaaag
      721 gcatgttgaa ttttgaaggg gaaatttctc taaagtttcg agctataata tccccgtaat
      781 ttacagatgt cgcatatatt tttatcagta tttcattatc cttcggggct ggttttgtta
      841 cctcttcgag atgaagaaca tcggggggtc cgtattttgt gtaaacaatt gctttcataa
      901 gtgttcctct atagttatta ctacatttcc ctttttatgt cctttttcca catatctatg
      961 agcttcgaca atctgttcca gaggatacgt tctatcaatg actgatttta actttccagc
     1021 ttcaacaagc tctttgaggg aaactaaatc aacagtcttt aactttaagg tgtctgatga
     1081 ggtaagaacg ttaagataaa ttccagtctt ctttagagat tttttaactc gtgaatatgc
     1141 aattttgcct actgcatcaa agataacatc gtaagtctca tcgtttttgg taaaatcgga
     1201 tgctgtataa tcaatgattt tatcagctcc cagggatttt accatttcta agttcgcagt
     1261 actgcataca ccggtaactt ccgccccaaa attcttggca agttgcacag caaaagtgcc
     1321 tacacttcca gaagctccat agataagaac tttttgacca ctttggatat ttgcttttct
     1381 aagaataagc aatgcggtta ttcccccatt gggaaccgga gcggcttctt cataggtcat
     1441 attggccggt tttattgcta acaccccgtc ttccggtagg catatatatt cagcataagc
     1501 accgaatctc atcaactcgg tagatgcaaa aacgggatcg cctctcttaa acaacgtaac
     1561 atctttgcct actgcttcaa tttcacctgc tagctccatc cctaatattt tttttcgggg
     1621 tcctgtgaaa cctataatca ttctcattat gggtttaaaa aataaatcct ttacaaatcc
     1681 taaaccaggc tctaaacgtc gaatcttagt atcccctcct atatgtactg ttgttgcgtg
     1741 tatttttatc agcacttcat tatctttcgg agtaggtttc tccacctctt tgagctgaag
     1801 aacctccggc ggtccatatt ttgtgcatac aattgctttc ataagtattc ctccaatttt
     1861 atatggttat gttgttatgt tctctatttt tttctttgct gcaatgaaat attgttgtcc
     1921 acttttatgc aaacattcgg tttcaacaat ctcaaaattt ccatcagtca ttaaatcttc
     1981 taattcagtg atcttaaatg atgtgatctg aggaattaat ccgattttgc tcagaggcga
     2041 taacagaatt cctaaaaatg tccctttaat acaaggtgtt gcagaaataa tcaatccacc
     2101 cggtttcaat aattcattta tcctttgcat aacctttggc gtatcttcca ctaaatggag
     2161 caggtagaaa cataagatca catcaaatga tccttttttt aatttttcat caaatattgt
     2221 tgtttgcgcg aaatctatgt tcttaacttt aagttctaca gtttttcctt tcgcagcttc
     2281 aatcattttg gatgagatat caatacctgt gactgtttta acactgctcg caatttcaat
     2341 ggctgctgtt ccagttccac atccataatc taaaacatta tcactaattt tcagtcggtt
     2401 tctagttttt tcaataatct taatattagt cggttcatct tttctttcta cccgatcaaa
     2461 atacttcgct atcctatccc agaacttttc tgatttattc attctttaca tccctctgtt
     2521 gaacatttac gcctttaatt aaaagccaaa atataatcat caactccccg ataaatgtat
     2581 atgacgagat tgccaaaatg aggacaaaat atgagccccg ctagttgcaa tgtataaaaa
     2641 tctttatagt tatacttaga taaaatctta ttgatgatgc caatttggat atgtaaaagc
     2701 tgaaaatagt ttttgaaaaa atcaatatca attgacacct ggctatgctt tctattcttc
     2761 ataacactcc ttttcccgta tactggctga attcccattt ctcccttttg atccctgcaa
     2821 gtacacttct tgccgcatcc tctggcagcg ctagcataag cgcaccgata tgcttacaaa
     2881 atcttttctc tctttgtgtt tgtctccaat caccacaatc atgaagtata aaccgccttt
     2941 taagatctac gtaaactgaa taaccccgaa catcagcaat gatcttttcc atgctcaatt
     3001 cgattatttc aatgtccttt atctcctttg ccctatcaag ccttaaagca tcaatatact
     3061 ctgaaagcaa agtcattaca tcctcttttt ccgattcaag aaaatcctcc agtgtcgtag
     3121 caacaatttt ttcttttacc ttacttttcc ggtaatccga aatatgctgc cttgcctctt
     3181 tctgttcagg agtagctact cccagcattt ccaatacatc aatgcagttc tcaggtgcgt
     3241 agccatggta atggttgtta aagtagccat aaacctctac gttatgcgac atctctttaa
     3301 ttcttggcaa ccattgcgca agttcatctt ttgaatagcg gtaattatac cacatctttt
     3361 tacctctccc atgccagcgc acataggcga tatccgaagt ccgcctgatt tcaggtggga
     3421 gcagcggctc atctacagcc acagcggcaa cattatgact ggagagcata tcatgcgctt
     3481 catcagtaag ccaggtctca ttcctgtact caagtgcaaa tctcatatca agaggcagta
     3541 ttctcaggaa tgcttctatc ctgtcttttt tgaatttcat tccgggtggt agctggacca
     3601 ggatgcaggc aagcttttct gtttccttca gcggtttcat gagttcgcag aatcttcgca
     3661 aatcatcctc cacccctttt gatagatcaa gcattttttc atgcgttatc agacggttaa
     3721 gcttcactgc aaatttaaaa tcaggaggtg tatgctttgc ccagccaaac acaattccag
     3781 gagcagggta actgtagaaa gtagaattga tctctgctgt gttaaaaata cgcgaatatg
     3841 ctttgagttt agactcatct gtctttttat agaaaacatc tgcccagtcg cgataatccc
     3901 agcctgagca tccgagatga attgagccca taccaattaa ttatatcctt tagaattaat
     3961 acttatgtct atgctataca ttggcgccgg aggatgggcg tatttcaaag tacccgggtt
     4021 ggattccctc gctgcctact caaaggcata tgacagaatc gtggggttaa ggggtgagcg
     4081 caagcctccg gcttcagccg tggggatagc gcacccctta gtatcgaaat gtctatcccc
     4141 gaaaaaagag ttgtcgattt tgcatttttt agggatagac aggcataaat aacatcaaaa
     4201 catatatagg cttgtcgatt ttgaatgaaa aaacctaaat caaaaactcc atcgttcata
     4261 acagaactcc cgttaaaaac cacatcggca gatgaatcaa ctatacttgt acgtctggat
     4321 gctggcagac agttatatca tgcctgtctt ggggaaggac tggaacgtct tgcacttatc
     4381 cagcaatcaa aagaatatca ggaaatccag aaactcccga aaacaataga tggtaagcag
     4441 aatactgaac ggacaaaagc attcaatgat cttaacagga aacatgggtt caccgattat
     4501 gctcttcaac attatgcaac aggaataagg aatagctgga tggaaaaaca tatccacgct
     4561 catattgcac agaaattagc aacaagagcc ttcaaaggcg tccagaaaaa agcatttgga
     4621 atagcaaaga acgtgagatt taagcgtaaa aaccaattcg attccctgga aggaaaaaat
     4681 gaaaccggat taatattcaa gaacaatgcc ctgttctggt caggtcttga aatcccatgc
     4741 atcatcgacc ccaaaaaca
//
LOCUS       DATGBN010000357         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_184014, whole genome shotgun sequence.
ACCESSION   DATGBN010000357 DATGBN010000000
VERSION     DATGBN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184014"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VKL21_08820"
     CDS             <1..896
                     /locus_tag="VKL21_08820"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HMB45911.1"
                     /translation="NGARLVESGWRWYYSYNLPEDRILPELLEGQSLRVKDVKLEGKE
                     TQPPSRYGQGHLIKIMEDLGLGTKSTRHEIISKLFSRAYVHGNPLQPTKTAFAVVEAL
                     EKYANTITKPDMTSKLEKDMDEISEGKITEDFVVKESRDMLEEVFKDLTRNKDLISES
                     LRNGLYEDRVIGTCQKCSSDLIIRKSKKGSRFIGCSGYPNCDFTLPLPRIGQIVVTEK
                     QCKDHGLFHIKILNKGKRPWEIGCPHCNFNEWQKKVEEEKKNGGGKESAVKTKGSEKS
                     TDKPKSPKKPKPLNKKNHTER"
     gene            941..1408
                     /locus_tag="VKL21_08825"
     CDS             941..1408
                     /locus_tag="VKL21_08825"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HMB45912.1"
                     /translation="MVLFELLISYGMMGLFIIALISSIIPIPTEPVVFGLLDVGKKPE
                     FVLIILIIGSIAGAYIGYLLGKYKLRKLIPFHDRKKEIMMQIQFRKYGALLLLVSPWI
                     PIIGDLAPMVAGIENYESRKFLLVISVAKTIKCIGIIYLSIKVIDWWTPFIKL"
     gene            1491..>2618
                     /locus_tag="VKL21_08830"
     CDS             1491..>2618
                     /locus_tag="VKL21_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMB45913.1"
                     /translation="MPKREDIHKILIIGSGPILIGQAAEFDYSGTQACKALRQLGYEI
                     VLVNSNPATIMTDPGMADRTYIEPLNADMLTRIIEKERPDAVLPNLGGQNGLNLTSEL
                     NKKGILEKYGVKIIGVQADAIERGEDRIAFKETMTQLGLEVPHSEATYTVEGAEKIAK
                     EIGYPVVIRPAYTMGGTGSGFAYNIDELRIIAARGIAASLIGQILIEESVLGWEELEL
                     EAVRDAQNNKITVCFIENIDAMGVHTGDSFCTAPMLTINKELQERLQEYSYRIVEAIG
                     VIGGTNIQFAHDPRTDRVVVIEINPRTSRSSALASKATGFPIALVSAKLAGGLLLRDI
                     PYWREGTLDKYTPPGDYVVVKFARWAFEKFPGSIDKLGTQMR"
BASE COUNT          853 a          522 c          623 g          620 t
ORIGIN      
        1 cgaacggtgc taggcttgtg gagtccgggt ggaggtggta ttattcatat aacctgcccg
       61 aagacagaat actgccagag ttgctggaag gccagtccct gcgggtaaaa gatgtaaaac
      121 tggaaggaaa agagacgcag ccaccctcaa gatatgggca gggacatctg atcaagataa
      181 tggaagatct ggggttgggg acaaaatcaa cacggcatga aataatatcc aagctttttt
      241 caagggcata tgttcacggc aatcccctcc agcccacaaa aactgcattt gcagtggttg
      301 aggcgcttga aaaatatgca aacaccatca ctaaacccga catgacaagc aaacttgaaa
      361 aggatatgga tgagatttcc gaaggcaaga tcactgaaga tttcgtagtc aaggaatcaa
      421 gagatatgct tgaagaagta ttcaaggatt tgaccaggaa caaggatctt ataagtgaat
      481 ccttgaggaa tggattatat gaagacaggg ttatcggtac atgccagaaa tgttcttctg
      541 acctcattat caggaaatcc aaaaaaggtt cgagatttat cggatgttcg gggtatccaa
      601 attgtgattt cactcttccc ctgcccagaa taggacagat tgtagtcact gaaaaacaat
      661 gcaaagacca tggtcttttc catatcaaaa tattgaacaa aggaaagcgt ccctgggaaa
      721 tcggatgccc tcactgtaat ttcaatgagt ggcagaagaa ggttgaagaa gagaaaaaga
      781 atgggggggg taaagagtca gcagttaaga caaagggtag tgaaaaaagc acagacaaac
      841 ccaagtctcc aaaaaaacca aaacccctga ataagaaaaa ccatactgag cgataaattg
      901 cgcatattat ttatatacaa cacccgatat tagcaacaaa atggtactat ttgaactttt
      961 aatatcctat ggtatgatgg gactatttat catcgctctt atctcatcca taataccaat
     1021 acccactgaa cctgtggttt tcgggttgct ggatgttggg aaaaaaccgg agttcgtctt
     1081 gataatattg ataataggat caatagcagg agcatatatt ggatatctat tgggaaaata
     1141 taaacttcga aagctcatac cattccacga caggaaaaaa gaaataatga tgcaaattca
     1201 atttcgaaaa tatggggcat tactcctgct tgtttctcca tggataccta tcattggaga
     1261 tcttgccccg atggtcgcag gaattgaaaa ttatgaatca agaaaattct tgctcgtgat
     1321 ttcagttgca aaaacaataa aatgcatagg gatcatatat ttgtccataa aggtcattga
     1381 ttggtggact cctttcatca aattatgatc aggttatatc atatcctgaa aaaaaagcaa
     1441 atcacaagta taataaatat taaatatctc tatggagacg gtgaaagaaa ttgcctaaac
     1501 gtgaagatat ccacaaaata ctgatcattg ggtctggccc tatccttatc ggccaggcgg
     1561 cagaattcga ttattcagga acacaggcct gcaaagcact gagacaattg ggctacgaga
     1621 tcgtgctggt caattccaat cctgcgacga ttatgactga tccgggaatg gcagatagaa
     1681 catatataga acccctcaat gcggatatgc tcacaaggat catagagaaa gaacgtccgg
     1741 atgctgtact cccaaacctt ggcggccaga atggattgaa tctgacatct gagcttaata
     1801 aaaaagggat acttgaaaaa tatggagtaa agatcatcgg cgttcaggcc gatgctatag
     1861 agcgcggcga agataggatc gcttttaagg aaaccatgac gcaacttggc ctggaagtac
     1921 cgcatagcga ggcgacatac acggtcgaag gcgctgaaaa gatagcaaag gagatcggat
     1981 atccggtcgt gatcaggccc gcgtacacca tgggaggaac aggaagcggg tttgcttata
     2041 atattgatga gcttcgaata attgctgcac gcgggattgc agccagtctc atcgggcaga
     2101 tccttatcga ggaatcggtt ctgggctggg aggaattaga actcgaagcc gtaagggatg
     2161 cgcaaaacaa caagataaca gtctgcttca tagaaaatat agatgctatg ggtgtgcaca
     2221 caggcgatag tttctgtacc gcccctatgc tcacgatcaa taaagaactc caggaacgac
     2281 tgcaagaata ctcataccgg atagttgagg ccatcggggt gatagggggc acgaatatac
     2341 agttcgctca cgacccacgg acagatcgcg tggtagttat cgagatcaat ccacgcactt
     2401 cacgctcatc tgcgcttgcc tccaaggcta caggatttcc tatcgcactg gtatcggcca
     2461 aactggcagg aggccttctt cttcgggata ttccttactg gcgcgaagga acgctcgata
     2521 aatatacccc gcccggggat tatgtcgtag taaagtttgc caggtgggca tttgagaaat
     2581 tcccggggtc gatcgataag ctcggtacac agatgaga
//
LOCUS       DATGBN010000358         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_300496, whole genome shotgun sequence.
ACCESSION   DATGBN010000358 DATGBN010000000
VERSION     DATGBN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300496"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..415
                     /locus_tag="VKL21_08835"
     CDS             137..415
                     /locus_tag="VKL21_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMB45914.1"
                     /translation="MECTIDIGEMIISREKLKRPESYQEVFLILGEQGILPENFAIKF
                     APSAGFRNRLVHMYAKIDIEKLHYYLQNNIGDIEQFSKYIAQYLIKRR"
     gene            624..779
                     /locus_tag="VKL21_08840"
     CDS             624..779
                     /locus_tag="VKL21_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45915.1"
                     /translation="MCPQCFQVPEFVFIDCVHMAASFSARALPPLILLSSLREGAKSD
                     DAISEVL"
     gene            874..1089
                     /locus_tag="VKL21_08845"
     CDS             874..1089
                     /locus_tag="VKL21_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HMB45916.1"
                     /translation="MAETIPAIYEDGMFKPLQKVDIPEHKHVHIMLIPDEEDTLINAQ
                     KKELSTIIGIGSSGKTDIARKHDNYLY"
     gene            1101..1505
                     /locus_tag="VKL21_08850"
     CDS             1101..1505
                     /locus_tag="VKL21_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB45917.1"
                     /translation="MAIFVDTSAWYALLDKTDSDHVSAVEFKESLNHSILTTNYVADE
                     IITLTKSRLGYMNAVEIGNKLWNETIATLIRITPSDEKKAWDIFMKYHDKGFSFTDCT
                     SFAVMERFGIIEAFAFDEHFDQYENIIRLPKY"
     gene            complement(1786..2190)
                     /locus_tag="VKL21_08855"
     CDS             complement(1786..2190)
                     /locus_tag="VKL21_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB45918.1"
                     /translation="MSLLIDTSALVSARNADDKNHNKALDIMIRALKGDFGKLYISDY
                     IFDETVTIAYIRTGNKNFVKDIGIFARTKPIIFRFIEPIDFERAWELYLQFQDKNFSF
                     TDCTNIALMERHGIDTLFTYDSEFNGIVSIIG"
     gene            complement(2187..2450)
                     /locus_tag="VKL21_08860"
     CDS             complement(2187..2450)
                     /locus_tag="VKL21_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45919.1"
                     /translation="MAENIGVKISSDTKIILDRMYHQLRSSGIKITEKKILDILITNT
                     DVTTIKKYLKKEDDTALQMLKKPVQWGVADSSEDIDRYLYGNP"
     gene            complement(2538..>2766)
                     /locus_tag="VKL21_08865"
     CDS             complement(2538..>2766)
                     /locus_tag="VKL21_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HMB45920.1"
                     /translation="VVELALGIVPELKIHNGIRKYILRLAASNILPDELVWKEKKAAQ
                     YSSGVYSAMEKLARRNGYRGALGIYLRELKQ"
BASE COUNT          838 a          563 c          538 g          827 t
ORIGIN      
        1 tcagcgatat taatcaagaa taaatcaatc agtaccgtag gaactacggg acttcaagcc
       61 cggccaagta atctcaatag agaaccaatg aagcgggaag ctcgcctttt caaaggggag
      121 tagttcacag atcgcattgg aatgtacgat agacattggt gaaatgataa tttcaagaga
      181 aaaactgaaa cgaccagagt cttaccagga ggtgttcttg atccttgggg agcagggtat
      241 tttgccggaa aattttgcta taaaatttgc accctctgcg ggattcagaa acagacttgt
      301 tcacatgtac gcgaaaatcg atatcgagaa gctccactat tacctgcaga ataatattgg
      361 agatatagaa caatttagca aatacatagc gcagtatctg attaaacgca gatgaaacaa
      421 atcacaaaaa gtctgtaaat ccgtgggtat ccgcacaatc ttgtgtcatc cgcgttcctt
      481 ttgtctggat gaatactact tcatcaaacc aaaacaatca tgttcgcata cgcacctttc
      541 catatcttgc tgccataact gcgtgaaaga agaggaggac ggcggtattc tgtaaacctg
      601 gatgcatcgc cggtggagga cgattgtgcc cgcaatgttt tcaagttccg gaatttgttt
      661 tcattgattg cgttcatatg gcagcatcgt ttagcgcgcg tgcgctgcca ccccttatcc
      721 tgctgtcaag cctgcgggaa ggagcaaaat ccgatgatgc cattagtgaa gtattatgat
      781 tgattcaacg gcgtcttagt aaaatggctg ctcgaagaaa acgagcagaa attggaggat
      841 atctttattt aaaatcaggt aatcatatat atcatggcag aaacaatacc cgccatttac
      901 gaggatggca tgttcaaacc ccttcaaaaa gttgatatcc cagaacataa acatgtacac
      961 atcatgctta tcccggatga ggaagacact ctcataaacg cccagaagaa agaattatcg
     1021 acgataattg gcatcggaag cagtggtaag acagatatcg ctcgaaaaca tgataattat
     1081 ctttactgaa gtgatacttt ttggcaatct ttgttgatac aagtgcatgg tatgctcttc
     1141 ttgacaaaac ggattctgat cacgtttctg ccgtagagtt caaagagtca ttgaatcatt
     1201 cgatattgac cacgaattac gttgctgatg agataattac actgaccaaa tctcgtttgg
     1261 gctatatgaa tgcagtcgag ataggaaata aactttggaa tgaaacaata gcaactctta
     1321 ttcgcataac accatctgat gaaaagaaag cctgggatat ttttatgaaa tatcatgata
     1381 aaggtttcag ttttacagat tgtacaagtt ttgcggttat ggaacgattt ggaatcatcg
     1441 aagcttttgc ttttgatgaa catttcgacc aatatgaaaa tataatacga ttgccaaaat
     1501 attaatcaga tgaaatgccc ggagcaatta ttgcggatgc tctctatttt ttcattaata
     1561 cacgcaagta tggatggttg aataatatgc aaccggtggt ttcatgttaa accccaccag
     1621 cggcggcgca tccgctgggg tctggggtcg caccccagcg ctgttaagat aatgttgacc
     1681 gaaagtgttc acatacgcac acttttccat atcttgctgt cacacctgtg gaaaggagca
     1741 atccgatgat gtggactctg atcaatacgc gtatttgata atattttagc ctattatact
     1801 tacgatccca ttaaattcag aatcataggt gaaaagagta tcaataccat gcctttccat
     1861 aagtgcgata tttgtgcaat ccgtaaaact gaaattcttg tcttgaaatt gcaaataaag
     1921 ttcccatgcc ctctcaaaat cgataggctc aatgaaacga aatataattg gttttgttct
     1981 cgcgaaaatt cctatatcct tcacaaaatt tttattacca gttctgatat aagcaatagt
     2041 cactgtctca tcgaaaatat aatcgcttat atacaatttg ccaaaatcgc cttttaatgc
     2101 cctgatcatg atatcaagcg ctttattatg gtttttatcg tcagcatttc tggctgaaac
     2161 aagggcagaa gtgtcaataa gaaggctcat gggtttccat aaagataacg atctatatct
     2221 tcagaagaat ctgcaactcc ccattgcaca ggttttttca acatctgaag ggcggtatca
     2281 tcttcctttt tcaagtattt ttttatggtt gtcacatctg tattggttat gagaatgtca
     2341 aggatctttt tttctgtaat ttttatcccg ctgctgcgta attggtgata cattctatca
     2401 agaatgatct tagtatctga acttatttta acacctatgt tctcagccat gtttatctac
     2461 tttctacttt ctactaaata aaagctcttc cataacgaaa caaaaatctt ccgtctttat
     2521 gcgcaggagt gtcaatctta ctgctttaat tctctcagat atattcctaa agcccccctg
     2581 tatccgtttc tcctggcaag cttctccatc gccgaataaa cgccgctgct gtattgcgcc
     2641 gctttcttct ctttccatac aagctcatca ggaaggatat tcgaagctgc aagccttaag
     2701 atatatttgc gtatcccatt atgtatcttg agttcaggga cgatccccag ggcaagttcc
     2761 acgact
//
LOCUS       DATGBN010000359         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_456033, whole genome shotgun sequence.
ACCESSION   DATGBN010000359 DATGBN010000000
VERSION     DATGBN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456033"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..361
                     /locus_tag="VKL21_08870"
     CDS             104..361
                     /locus_tag="VKL21_08870"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45921.1"
                     /translation="MQQYSTQDLKDRTHSCPYCGSVMPRDYNASQNIKLRGIEMVGWG
                     TPEPGFLDIEKLNTLAEIRTTILDINNPEQVFVNEPRISRL"
     gene            449..697
                     /locus_tag="VKL21_08875"
     CDS             449..697
                     /locus_tag="VKL21_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45922.1"
                     /translation="MNERKYTRWKKCLICDDFVKLMFPEDAKNMLLIYEPIEGMSNES
                     RNWKKKQLGYIHRSCLQKASSLKQKFNEDENSRIAEVL"
     gene            697..2133
                     /locus_tag="VKL21_08880"
     CDS             697..2133
                     /locus_tag="VKL21_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HMB45923.1"
                     /translation="MAYRDLREFIELLEKRGLLKRIKIEVNAELGITEILDRCVKNNG
                     PAILFENVSGCKTPVFANAFGSMERMCLALGVNDLDEIGARIRKLMEFEAPSGLWEGI
                     KKLPDLVELTSLAPKHVKSGPCKEVILRDNFSLYDFPILKCWPGDGGPFITLPLVFTK
                     NPKTGKQNVGMYRMQVYDGKTTGMHWHIHKHGARDYADGNGKIEVAVAIGADPSVVYC
                     ATAPLPDNIDEMMFAGFLRKKNVELVKCEKVDLYVPSHAEIVLEGYVDEKERRTEGPF
                     GDHTGYYSLADEFPVFHITCITHRKNPIYHATVVGIPPMEDAFLGKATERIFLPLMKA
                     QLPEIVDINLPVEAVFHNLCIVSIKKRYPGQAKKVMFALWGMGQMMFAKTIIILDDDV
                     NVQDMREVIWATTTRIDPAMDVTIIDKAPTDTLDHASPLPNLGSKMGIDATRKGHDEG
                     FNREWPDALKMDAAVKARIDKIWKELGL"
     gene            complement(2171..2962)
                     /locus_tag="VKL21_08885"
     CDS             complement(2171..2962)
                     /locus_tag="VKL21_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HMB45924.1"
                     /translation="MTDINYLIAMINLYFIGSAGSGKSSLTGAFFGWMENQRYDAITV
                     NLDPGIEKAPYVPDVDIRDWIKLDDVMEEYNVGPNGAQVLAADLLALNIHEMKEIIES
                     FESDYVIFDTPGQMELFVLRQSGKFLIDTLGADQSIIGFLYDPIISKTPSGFISLMLQ
                     AASVQVRFNVPFLNILPKSDLLKEEERNKIINWSTNMVDLEDEIHNEAPSMQSQLSIE
                     FLSALKSFGASQNIIPASSTYGSGMEDIYNTVQQIYFGGEDLSRD"
     gene            2980..3858
                     /locus_tag="VKL21_08890"
     CDS             2980..3858
                     /locus_tag="VKL21_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HMB45925.1"
                     /translation="MLKKLKLYADFVVLKHTLFAIPFAYLGAFLASNGVPAFRILFWV
                     GLAFLGARSAAMALNNLIDKDIDAKNPRTSNRELPAGRISLIEVWAIIMISYFFLFYS
                     AYKLNPLCLLLSPVIPVTSIIYPYLKRCLPVSHFVLGLNLGYAPVGGWLAVTGQFNIP
                     FGANEIAMLILMFAVTLWVAGFDIIYSLSDIDFDLKNKLYSVPAVFGVKRALFVSFLS
                     HILMLALLVLLMFVLNLGIIFKIGLVVIAALILYEHSLVKADNFSKVQVAFFNVNAAV
                     SLSMLVFTVMDILLRY"
BASE COUNT         1141 a          781 c          870 g         1090 t
ORIGIN      
        1 cttcatgggg taaatttgca aactatgtat tatacaaagc cgagaacgct ggtaaatatg
       61 cagttcaagt catagcaaaa ggaactacac aggattgtag tgtatgcagc aatacagtac
      121 ccaagacctg aaagatagga cacattcatg tccttattgc ggttcggtca tgccgagaga
      181 ctacaatgca tctcaaaata ttaagctcag aggaatagaa atggtagggt ggggcacacc
      241 cgaaccaggc tttctcgata tagagaagct aaatacgctt gcagagataa gaaccactat
      301 tctggatatc aacaatccag aacaagtttt tgtcaacgaa ccaagaatct cccggcttta
      361 gctgtggaga gtgtcaaccc tgcacagttt tgtgatcaaa ctaccgtaac ggcataggta
      421 tatattcccg taacgcagtc aggataatat gaatgaacga aaatatacca gatggaaaaa
      481 atgcctgata tgcgacgatt ttgtgaaact catgtttcca gaggacgcaa aaaatatgct
      541 cctcatctat gaacccattg aaggaatgag caatgaatcc aggaactgga agaaaaaaca
      601 acttggatac atacaccggt catgtcttca aaaagcgtcc tctttgaaac aaaaatttaa
      661 tgaagatgaa aacagtagaa ttgctgaggt cttatagtgg catacaggga tttacgtgaa
      721 tttattgaac tcctggaaaa aagaggtctt ctaaagcgga taaagattga agtaaacgcg
      781 gagcttggaa ttactgaaat tcttgaccga tgtgtgaaaa ataacggccc tgctattcta
      841 tttgaaaatg tttcaggctg caagacacct gtatttgcaa atgccttcgg cagtatggaa
      901 agaatgtgcc ttgcgcttgg tgtaaatgat cttgatgaaa ttggagcaag gatccgcaaa
      961 ctcatggaat ttgaagctcc atccggatta tgggaaggaa taaagaagct cccagatttg
     1021 gttgaactta catcattggc tccaaaacat gtgaaatcag gcccctgcaa agaagtaatt
     1081 ctgagggata acttttctct ttatgatttt ccaatattga aatgctggcc gggagatggg
     1141 gggcccttca taacacttcc ccttgtattc acaaaaaatc caaaaaccgg gaagcagaat
     1201 gtgggaatgt acaggatgca ggtatatgac ggcaagacca caggcatgca ctggcacata
     1261 cacaagcacg gcgcgaggga ttatgccgat ggtaacggaa aaattgaggt tgcagtagca
     1321 ataggagctg atccttctgt tgtgtattgc gctacggctc cccttccgga taatattgat
     1381 gagatgatgt ttgccggttt cctgcgaaag aagaacgtcg agcttgtaaa atgtgaaaaa
     1441 gttgaccttt atgtgccctc tcatgccgag atcgtacttg aaggctatgt ggatgaaaaa
     1501 gagcgccgca cagaaggacc ttttggggac catacaggat attattctct tgcggatgaa
     1561 ttcccggtat tccatattac ctgcattaca caccggaaaa atccaattta ccatgcgacc
     1621 gttgtgggaa tccccccaat ggaagacgcc tttctcggaa aggcgactga acgtatattc
     1681 ctgccgctca tgaaagcgca gcttcctgaa attgtggata ttaacctccc tgttgaagcc
     1741 gtattccaca acctttgtat cgtatcaata aaaaagcggt atccagggca ggcaaaaaag
     1801 gtcatgttcg ctctgtgggg aatgggacag atgatgtttg caaagaccat aatcatactt
     1861 gatgatgatg tgaatgtcca ggatatgaga gaagtgatat gggcgacaac aacgagaatt
     1921 gatccggcca tggatgttac tattatagat aaagcgccga cagatacact tgaccatgct
     1981 tcgcctcttc ccaaccttgg ctctaaaatg gggattgacg ccacgcgaaa aggacatgat
     2041 gaaggtttta atcgagaatg gccggatgct ttgaaaatgg atgccgctgt taaagcgcga
     2101 atcgataaga tctggaagga gctaggattg tgaaccacca tcagacagtc cgatggcatc
     2161 tacaccatat ttaatccctg ctcaaatcct cgcctccaaa atatatctgc tgcactgtgt
     2221 tgtatatgtc ttccatccct gaaccataag tcgatgatgc agggataata ttctggcttg
     2281 ctccgaatga ttttaaagca gaaaggaact ctatacttag ctggctttgc attgagggtg
     2341 cctcattatg gatttcatct tcaaggtcca ccatattcgt gctccaattg attatcttat
     2401 ttcgttcctc ttccttcagc agatcggatt ttggcaggat attgagaaaa ggaacattga
     2461 acctgacctg cacagaagcc gcctggagca ttaatgaaat aaaacctgaa ggggtctttg
     2521 aaattatggg gtcgtacagg aaaccgataa ttgactggtc ggctccaaga gtatcaataa
     2581 gaaatttacc cgactggcga agcacgaaca gttccatctg tccgggtgta tcaaaaatga
     2641 cataatcgga ctcaaagctt tcgattattt ctttcatttc gtggatattc aatgcaagga
     2701 gatcagcagc aagtacctgg gcgccattgg gtccaacgtt atattcttcc atcacatcat
     2761 ctaattttat ccagtcccgg atatcaacat caggaacata aggagctttt tcgatgcccg
     2821 ggtcgagatt taccgtaatt gcatcatatc tctggttttc catccatccg aaaaaagcgc
     2881 ctgtaagtga agacttgcca cttcccgccg agccgataaa ataaagatta atcattgcta
     2941 tcaggtagtt aatatcggtc aagagagaaa agatttttga tgttgaaaaa attgaaatta
     3001 tatgcggatt tcgtggtgct caagcatacg ctttttgcta tcccttttgc ctatcttgga
     3061 gcttttcttg caagcaacgg cgttccagcc ttcagaatcc tgttctgggt gggacttgcc
     3121 ttcctgggag cgagaagcgc cgcgatggcg ctgaataacc ttattgataa ggacattgat
     3181 gcaaaaaacc ccagaacctc aaatcgcgag ctaccggctg gcaggatatc attaatagaa
     3241 gtgtgggcaa taatcatgat atcatatttc ttcctatttt actcagccta taaattgaat
     3301 ccattatgcc tgttgctttc gcctgttatt cctgttactt caataatata tccatatctt
     3361 aaaagatgcc ttcccgtgtc acatttcgtc ctgggcttga accttggtta tgcgcctgtg
     3421 ggaggatggc ttgccgtaac cggacaattc aatattcctt ttggcgcgaa tgagatcgcg
     3481 atgttaattc ttatgttcgc agtcacttta tgggtagccg ggtttgatat aatatattcg
     3541 ctttcggata ttgattttga ccttaagaac aagttgtatt cggtgccagc tgtttttgga
     3601 gtaaagagag cgctttttgt ttcattcctg tcacatattt tgatgcttgc acttcttgta
     3661 ttgttgatgt tcgtgctaaa cctcgggatt attttcaaga tagggcttgt agtaattgcc
     3721 gctcttatat tgtatgaaca tagtcttgtt aaagctgata atttttccaa agttcaggtt
     3781 gccttcttta atgtaaatgc ggccgtgagt ttgagcatgc tggtgtttac tgtgatggat
     3841 atattgttac gctattaaag acatctgtta agagaaaatc ca
//
LOCUS       DATGBN010000360         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_28499, whole genome shotgun sequence.
ACCESSION   DATGBN010000360 DATGBN010000000
VERSION     DATGBN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28499"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1106
                     /locus_tag="VKL21_08895"
     CDS             153..1106
                     /locus_tag="VKL21_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMB45926.1"
                     /translation="MLQKNVSKIIIGIVFVLMLLQITQPAASKAPDILNNSCADCHSK
                     FLFSSETQRQFVDIRIKHLESGISCSIVCHEDKLNKSTASTYALWSISTHALFEVTCE
                     KCHGGDPTKVLKQDAHVGISNISIQRANTPEMCGKCHTSELDEFKSSQHFKRLESNQE
                     ELPAPACITCHQAHSVRVLTASEIEDFCSNCHNNITGINPSVPKKAEAALSSVKELQF
                     EISRARSSVLAAKAMEKDVTAAQADLESASLILKNTPSVWHRFNLTYFDTEVQKGIND
                     ARNAEKAMPEGTAQPAPTKAPGFELLLLLSGLLAAYLLKRH"
     gene            1144..2067
                     /locus_tag="VKL21_08900"
     CDS             1144..2067
                     /locus_tag="VKL21_08900"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMB45927.1"
                     /translation="MSIKKTIIIAGFMFLFAIFAGAAADNSCLDCHEKLTAFSEKAKE
                     LNEVRIKHLQRDVACSIDCHATTIDEIAKSNYEQWTHSKHALFNITCDNCHSGNSSSD
                     IKEIAHNGVYRSSETNSTVFYRNVPETCGKCHSNELNQFKDSLHYQRLKALKQAPTCD
                     TCHEPHEFKVLNKTQFQDLCSSCHNTEMKIAPDIPEKAVNAFENAEKLKNEIRIAEDA
                     IKQAKQQGKDVSIARKELDSAIAIQDNLPILWHSFNLPHFQNVTDNGILYARTSQTDS
                     GVPPDKPSTPGFGTILSLTGIISIYLLVRRR"
     gene            2084..2431
                     /locus_tag="VKL21_08905"
     CDS             2084..2431
                     /locus_tag="VKL21_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45928.1"
                     /translation="MNKIRINKIIFLVALVLLLFLPSLTSARPEYAARESRNCPYCHT
                     RDGPPQLNEVGVYYVTHNHSIEGYVPTPKATPTPEPEKEIEIGVHMTTWDVGLRAMAI
                     ILLTLAVVYIIRL"
     gene            2441..>2680
                     /locus_tag="VKL21_08910"
     CDS             2441..>2680
                     /locus_tag="VKL21_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45929.1"
                     /translation="MHIEKIEYGPEKEGLWSQVAFSLIILMTFAAAGITGLIFWKILS
                     SDAQVASTRQFMEEVGKLFAYLLFDLAAIIYLYVKG"
BASE COUNT          882 a          538 c          584 g          676 t
ORIGIN      
        1 cgagagaaga agagaactga aagattgaaa ttattttata gtttaattaa tatatatttt
       61 tgatgcataa gtttaagtat ttggcaaata aatgagctac ctatatcatt attaataatt
      121 ttactaatag tgatgatgat aggagtgttc acatgctaca aaaaaacgtt tcaaagataa
      181 ttataggaat agtatttgtg ctgatgttac tgcagataac tcaaccggca gcatcaaaag
      241 ccccggatat actcaataac tcgtgcgccg attgccacag caaatttctt tttagttcgg
      301 agacacagag gcaatttgtg gatattcgca taaagcatct tgaaagcggg atttcatgct
      361 cgatcgtttg ccatgaggat aaacttaata agtcaacggc cagcacttat gcattgtggt
      421 caatatccac gcacgctctt ttcgaagtaa catgtgaaaa atgccatgga ggagatccaa
      481 caaaggtttt gaagcaggac gcacatgtgg gtatctcaaa tataagcatt caacgcgcca
      541 atactcccga gatgtgcggg aaatgtcaca cctctgaact tgatgaattc aagagctccc
      601 agcatttcaa aaggctggaa tcaaaccagg aggaattgcc ggcgcccgca tgtatcacct
      661 gtcaccaggc gcatagcgtc cgggttctga ccgcatcgga aatagaggat ttctgcagca
      721 attgccataa taatatcaca ggtattaatc cgtcagtgcc gaaaaaagct gaagccgcct
      781 tatcttcggt aaaagaactg caattcgaga tatcaagagc aagaagctct gtccttgcgg
      841 caaaagcaat ggaaaaggat gtgacagcag cacaggcgga ccttgaatcg gcaagcctga
      901 tattaaagaa taccccttca gtctggcaca ggttcaatct tacatacttt gatactgaag
      961 tacagaaagg aataaatgat gccaggaatg cagaaaaggc aatgcctgag ggaactgcgc
     1021 aacccgcgcc tacaaaagcg ccaggattcg aattactact gctgctatca ggtcttcttg
     1081 cagcatatct attaaaacgg cattgaaaaa tcgttattga gaattaaaga gaaaggtgat
     1141 tgaatgagca taaaaaaaac gatcataata gccggtttta tgtttttatt tgcgatattt
     1201 gccggggcgg cagcagataa ttcatgcctt gattgtcatg aaaaacttac ggctttcagc
     1261 gaaaaagcaa aagaactcaa tgaagtgagg ataaaacatc tgcaaagaga tgttgcatgt
     1321 tccattgatt gccatgcaac taccatcgat gagatcgcaa aaagcaacta tgaacagtgg
     1381 acacattcca aacatgccct gtttaatatt acttgcgaca attgccatag cggcaattcc
     1441 agttcggata taaaagagat agcacataat ggagtttacc gatcttccga aaccaacagt
     1501 actgtttttt acaggaatgt accggaaact tgtggtaaat gtcattcaaa tgaattgaac
     1561 cagttcaaag attcattgca ttatcagaga ttaaaggcac taaaacaggc gccaacatgc
     1621 gatacgtgcc atgaaccgca tgaattcaag gtactgaata aaacccaatt ccaggatcta
     1681 tgcagcagct gccataatac tgaaatgaaa attgcccctg acatccctga aaaagccgta
     1741 aatgcgttcg aaaatgctga aaaactgaag aacgagataa ggattgccga agacgccata
     1801 aaacaagcaa agcagcaggg aaaagacgtt tcaatcgccc ggaaagaact cgactcggcg
     1861 atagccatcc aggataatct tcccatactc tggcatagtt tcaacctgcc gcactttcaa
     1921 aatgtgacag ataacggtat tctttacgcc aggacatcgc agacggactc gggagtccct
     1981 cctgataagc cttcaactcc cggatttgga acaatattat ccctgacagg tataatttca
     2041 atttacctgt tagtgcgcag gagataaaag aggtgttatc agaatgaata aaataagaat
     2101 aaataaaatc atattcctgg ttgcattggt tctcctgtta tttcttccct ctttgacttc
     2161 ggccagaccc gaatatgcgg ccagggagag cagaaattgc ccatattgcc acacaaggga
     2221 tgggccaccg caactcaacg aggtaggcgt ttattatgtg acgcataatc attcgataga
     2281 aggttatgtt ccaacaccga aagcaacacc gacccctgaa cctgaaaagg aaatagagat
     2341 aggggttcat atgacaacat gggatgtggg actgcgggcg atggcaatta ttctgctaac
     2401 tcttgcggta gtttatataa taaggttgtg aggtagatcc atgcacatag agaaaattga
     2461 atatggaccg gaaaaagaag gtttgtggtc gcaggttgct ttttcattga taatcctaat
     2521 gacatttgcc gcagcgggaa taacaggttt gattttctgg aagatcttat ccagtgatgc
     2581 ccaggttgca tcaacaaggc aattcatgga agaggttgga aagctattcg catatctcct
     2641 gtttgacctg gcagccatca tttacctata tgttaaaggg
//
LOCUS       DATGBN010000361         4875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_67829, whole genome shotgun sequence.
ACCESSION   DATGBN010000361 DATGBN010000000
VERSION     DATGBN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67829"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VKL21_08915"
     CDS             <1..314
                     /locus_tag="VKL21_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HMB45930.1"
                     /translation="LIPRNRPKLAVAYSLAAKYLGMHYIYLEAGSGADKPVPVEMVAA
                     VKKAVGEKTKIIVGGGIRDGITAKERVKAGADMIVTGTIVEQVDDVKGKIEEIAEAIK
                     S"
     gene            422..550
                     /locus_tag="VKL21_08920"
     CDS             422..550
                     /locus_tag="VKL21_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45931.1"
                     /translation="MNTKISEIQQKRPSLIKNVGKKQPEALESWMIEEYYVPYQAS"
     gene            766..2268
                     /locus_tag="VKL21_08925"
     CDS             766..2268
                     /locus_tag="VKL21_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMB45932.1"
                     /translation="MLLNIERKAGGFHIEENSSELKPIKAIYAVLGTGGIGLALAKEL
                     ETRTKNIILVDKDSAKVKTLLEQNLNAVQGNIGDTGVISSLDIQNIQSVFIMSSDIEA
                     NKKALNFIRKEAPDVQIVARVNNYQEKEEMETAGADLVVLPSNLPIKAIASAIVQYIE
                     KMKSVKLAQELKKLINTVGEGKLAIIVHDNPDPDAISSAMGLKEIAASVGVKADILYK
                     GIIGHQENKAFVNLLDIEMDHSKDFKASDYKKIALIESSAPGVNNLLPQGTKVSIVID
                     HHQANMEDVEAEYIDIRPNIGATATIMTKYLQELEIPIKTELATALLYGIKVDTNDFR
                     RNTDPTDLTAAAFLFPLANHDIMNRIETPSKSTESIDILGEAIKNRQVKGSYLISNVG
                     TIRDRDTLAQAADYLLKLEGITTTLVFGLGEDTIYISGRSRDDRINIGKILADAFGAD
                     KAGGHALLAGAQIPLGVFSGTKDKQTLLKLAEEAVVKRFLTVIGMKKETG"
     gene            2345..>4875
                     /gene="alaS"
                     /locus_tag="VKL21_08930"
     CDS             2345..>4875
                     /gene="alaS"
                     /locus_tag="VKL21_08930"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMB45933.1"
                     /translation="MDEYQLNYFKENGFVRKQCPKCQKNFWTRDSETEFCGDPPCVSY
                     SFIGTPVFKKEYDISSMREAYLSFFEKHGHTRVNRYPVIARWRDDIYLTIASIADFQP
                     FVTSGLVPPPANPLTISQPCIRLDDLDAVGRSGRHLTTFEMMAHHCFNKRGQEIYWKD
                     RTVELCDSLLASLGLDLNAVTYKEAPWAGGGNAGPSVEVLVGGLELATLVFMDLKQTK
                     GGSIEIKGETYEKMDNYIVDTGYGLERFVWASKGSPTIYDAVFPKIVNELMDLAGIEH
                     SIENPEYARIFSQNAKLAGVMDISGQANLLQLRKKVADSIGTTVEKLQRIMVPVETVY
                     SITDHSRCLAFMLGDGIIPSNVKAGYLTRLVLRRTLRLMKELDIREPLIEIIEMQIRN
                     FPEYPEFREKLDTIREIVALEEEKYADTIERGTKLVKKTASHFKEKKQMIPLGELIQL
                     YDTHGIPPEITREVAKEAGVEVDLPDTFYSLVAQSHSKAQTEENEAIALELPPTKKLY
                     YEYPEQMEFEAKVIEVIENNVILDRTLFYPEGGGQPADHGTLCVNDHVANVVDAQNRN
                     GVIVHETDSDFGFKKGDTVKGMIDQERRMAHMRHHTATHIVNEAAKTVLGKHIWQTGA
                     QKSTDRARLDLTHYKRITDEEFKEIELLANKAVMKNQPVYIDWMDRIEAEQRYGFVLY
                     QGGVPPGKEIRILRVGNDVEACGGTHVSNTGLIGPIKLIKTERIQDGVERIEFSAGEA
                     AVKRMQERDELLNKASEALRVSPDQLPDTVNRFFDEWKDFKKENERLKAELAQARVKG
                     MMSDAVDINGLRVLAKKIPNADVEELIKAATEFSKNDDVVAILAS"
BASE COUNT         1514 a          977 c         1206 g         1178 t
ORIGIN      
        1 gccttattcc aaggaacagg ccaaagctgg ctgttgctta ttctctggcg gcaaaatacc
       61 tgggcatgca ttatatttat cttgaagccg gttcaggggc cgataaaccc gtacctgttg
      121 aaatggtcgc agcagtaaaa aaagcagtgg gagaaaaaac gaaaataatt gttggcggag
      181 gtatacggga cggtatcact gcaaaagaac gtgtaaaagc cggagcagat atgatagtaa
      241 ccggtaccat cgttgaacag gtagatgatg ttaaaggaaa gatagaagaa attgcagaag
      301 cgataaaatc gtaagaccac ttttatggtt ctataatagt aatggaatcg ataactttaa
      361 attcttataa ggactcaaat ctaatggaga ctgtcatcca gaatggatgc cattgatata
      421 aatgaacacc aaaatatcag aaatccaaca aaaacggcca tcattgataa aaaatgtggg
      481 aaagaaacaa cccgaagcgc ttgaatcatg gatgatcgag gaatattatg tcccatatca
      541 agcatcttaa tttttagtat ttaagggagg gagagaatgg aagtaaaggg agtatgcagt
      601 atctgtggaa atccgggaaa gttgcataca tgcatgctgt gtgggaattt agtgtgttcc
      661 caatgtatca cccggggtgg cgcctgcgtt cgctgttccc atggcaggtc attttcagat
      721 taaaaatgat gattgttcag tttaaatgcc aatgcatttt aatacatgtt acttaatata
      781 gagcgtaagg caggaggctt tcatatcgag gagaactcaa gtgaattgaa acccataaaa
      841 gcaatatatg cggttctggg taccgggggt attggtcttg ctctagcaaa ggaacttgag
      901 acccggacta agaatatcat ccttgtggat aaggattctg caaaagtcaa gacattattg
      961 gaacaaaacc ttaatgctgt gcagggaaat attggcgata ccggggttat atcaagtctg
     1021 gatattcaaa atatccaatc tgtttttatt atgagttctg acatcgaagc caataagaaa
     1081 gcattgaatt ttatacgaaa agaagctccc gatgtgcaaa tcgttgcaag ggtcaacaat
     1141 taccaggaga aggaagaaat ggaaacagca ggcgctgacc ttgtggttct tccttcaaat
     1201 cttcccatca aagccatcgc ttcagccatt gtacagtaca tcgaaaaaat gaaatctgtg
     1261 aaacttgccc aggaattaaa gaaattgata aatactgtag gtgaaggaaa actcgccatc
     1321 atagtccatg acaatcctga cccggatgcg atctcaagcg caatgggctt gaaagaaatt
     1381 gctgccagtg taggtgttaa agcagatatc ctttataaag gaatcatagg gcatcaagaa
     1441 aataaggctt ttgtgaacct ccttgatata gaaatggatc attcaaaaga cttcaaggct
     1501 tctgattata aaaaaatagc cctcatagaa tcttctgcgc ccggtgtaaa taatctgctt
     1561 ccccagggca caaaggtcag tattgtgata gaccatcacc aggccaatat ggaagatgtg
     1621 gaggcagaat acattgacat cagacccaac attggcgcga ctgctactat aatgacaaaa
     1681 tatctccagg aacttgaaat acctataaaa accgagcttg ccacagccct gttatatggc
     1741 attaaagtgg acacgaatga tttcaggaga aataccgacc ctaccgacct gacggctgca
     1801 gcatttcttt ttcctcttgc gaaccatgat ataatgaacc ggatcgagac tccatcaaaa
     1861 tccaccgagt ctatcgatat tcttggtgaa gctattaaga acaggcaggt aaagggaagt
     1921 tacctgatat caaatgtggg gacgatacga gacagggata cgctggcgca ggctgcggat
     1981 tacctgctga agcttgaagg aataactaca acgctagtat ttggtctcgg ggaagatacg
     2041 atatatattt ccggaaggag cagggatgac cggataaata tcggaaaaat cttagcggat
     2101 gctttcgggg ctgataaagc tggaggtcat gctttgcttg caggcgcaca gatccccctt
     2161 ggggtattca gcggcacaaa agataagcag acacttctca aactggctga agaagctgta
     2221 gtgaaacgtt tccttacagt gatcgggatg aagaaagaaa caggttgatt ttcggttatg
     2281 atttttcagg accattttcc agaatagtta tctattccct ttacaataac tacccccaga
     2341 tattatggac gagtaccagc ttaattattt taaagagaat ggatttgtgc gaaaacagtg
     2401 cccgaagtgc caaaagaact tctggacgag ggattcagaa actgaattct gcggagatcc
     2461 gccctgcgtt tcttattcat ttataggaac tccggttttc aagaaagaat acgacatatc
     2521 ctccatgagg gaagcatatc tttcattttt cgagaagcat gggcataccc gcgtgaaccg
     2581 ttatcccgtt atagcgcgct ggcgcgatga tatttacctg acaattgcct caattgcgga
     2641 ttttcagccc tttgtgacat ccggcctggt tccgccccct gcaaatcctc tcacgatatc
     2701 gcagccctgc attcgtcttg atgaccttga tgcagtgggc agaagcgggc gtcatctcac
     2761 aacctttgag atgatggcgc atcattgttt taacaagcgt ggacaggaaa tatactggaa
     2821 ggacaggact gtggaactat gcgattcttt gcttgcaagc ctgggtctgg acctgaatgc
     2881 agtgacctac aaggaagctc cctgggcagg agggggaaat gccggcccca gcgttgaagt
     2941 actcgtgggg gggcttgaac ttgccacact tgttttcatg gacctgaaac agacaaaagg
     3001 cggctcaatt gaaataaaag gcgaaacata cgaaaagatg gataattata ttgttgacac
     3061 tggttacggt cttgaaagat tcgtgtgggc atccaaaggt tcacccacga tatatgatgc
     3121 agtttttcca aagatcgtga atgaactgat ggaccttgcc ggtattgagc attcgataga
     3181 aaatccggaa tatgcccgga tattttcgca aaatgcaaag ttggccggag taatggatat
     3241 cagcgggcag gcgaaccttc tccagctaag aaagaaagtg gcggatagca tcggcacaac
     3301 cgttgaaaaa ctccagagaa taatggtgcc ggtcgagaca gtttattcga ttaccgacca
     3361 ttcaagatgt cttgcgttca tgctgggcga tggtataatc ccatcaaacg tcaaggccgg
     3421 atatctcaca agactcgtgc ttcgccggac gcttcgcctc atgaaagaac tggacattag
     3481 agagcccctg attgaaataa tcgagatgca gatacggaat ttcccggaat atcctgagtt
     3541 tcgggagaag cttgatacca tcagggaaat agtagcgctt gaagaagaaa agtatgcaga
     3601 taccattgag cgtggaacga aacttgtgaa aaaaacggct tcgcatttca aggagaaaaa
     3661 acaaatgatc ccccttggtg aattgataca gttgtacgat acgcatggga tcccacctga
     3721 gataacccgt gaagtggcaa aagaagccgg tgttgaagtg gatcttcccg atactttcta
     3781 ttcacttgta gcacaatcac acagcaaggc gcaaactgaa gaaaatgagg cgattgcgct
     3841 tgaattaccc ccaacgaaaa agctctatta tgaataccct gaacagatgg agtttgaggc
     3901 aaaagttatc gaggtaattg aaaacaatgt aattcttgac aggacattgt tctatcctga
     3961 aggcggcggc caacccgctg accacggaac gctttgtgtc aatgatcatg tcgccaatgt
     4021 tgttgatgca cagaatcgaa atggagtaat tgtccatgag accgactcag atttcgggtt
     4081 caagaaaggc gataccgtaa aagggatgat cgatcaggag cggcgtatgg cgcatatgag
     4141 acatcatact gccacgcata ttgtgaacga agctgctaaa accgtcctgg gaaagcatat
     4201 ctggcagacc ggggcgcaaa aatcaacaga cagggcaagg cttgacctca cgcattataa
     4261 gcgcattaca gatgaagagt tcaaagagat cgaacttctt gcgaacaaag ccgtgatgaa
     4321 gaaccagccc gtttatattg actggatgga caggatcgag gctgaacagc gttatggttt
     4381 tgtgctctac cagggcggcg ttcctcccgg caaggagata cgtattcttc gcgttgggaa
     4441 tgatgtggaa gcctgcggcg gaacgcatgt ttcaaatacg ggattgatag gccccatcaa
     4501 gctcataaag accgagagga tccaggacgg ggttgagcgt atcgagttct ctgcaggtga
     4561 agctgctgta aaacgcatgc aggaaaggga tgaattgctg aataaagcct cagaggcgct
     4621 tcgcgtatca cccgatcagc ttcctgatac cgttaaccgg ttctttgatg aatggaagga
     4681 cttcaagaaa gagaacgagc gcttaaaagc tgaacttgcc caggcaagag tgaaggggat
     4741 gatgagcgat gcagtggata ttaatggcct gagagtgctt gcaaaaaaga tccccaatgc
     4801 tgatgtggag gagcttatca aggcagccac agagttcagc aagaacgacg atgttgtggc
     4861 gatacttgca agcga
//
LOCUS       DATGBN010000362         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_146192, whole genome shotgun sequence.
ACCESSION   DATGBN010000362 DATGBN010000000
VERSION     DATGBN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146192"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VKL21_08935"
     CDS             <1..309
                     /locus_tag="VKL21_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMB45934.1"
                     /translation="SQVKNATLTIQSFSYINGTVTNASVPIPGVYVSTTGANYTTGSD
                     GNYSLSVPEGNYVVNAIKQPEFYDYTISGVVLNPSDTTIRDINLIRKPNGTISGVVTN
                     "
     gene            540..1985
                     /locus_tag="VKL21_08940"
     CDS             540..1985
                     /locus_tag="VKL21_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45935.1"
                     /translation="MPKKVLLITPPYHCGVLESAGSWMPLGMVYVAGSLQNSGYDVKI
                     YDAMTKFHTFDDIKKHITEYSPDAVGTAAYTSSLYDAISVLRIAKEVNPSIITMLGGI
                     HSNFCWEEILQKDGNIIDFIVRGEGEITTTELLNNVFAGKEPDVPGIAYSGDGKIIAT
                     KERPLIKDLDSLPAAWNLIEWKDYSFKTKKNSTLAVVSSSRGCTQKCTFCSQRLFWKE
                     KWRGRSPEKFVAELEYLNKTYGVDVAMIADETPTFERERWEKILDLLIEKKLDIEILM
                     ETRVDDILRDKDIMYKYSGAGILHIYVGAESASQDTLDRYNKNLKIEQSKEAIDLINN
                     EGIISETSFVLGMPDETPSSIDTAIKLAKYYNPDLAFFLAIAPWPYSDIYKELSPYIE
                     EFDYSKYNLIEPIVKPVNMTREELNKHLLRAFREFYMDKLSTIEEMSEFKKNYMIDVT
                     RLLINDSYLANQMRGEMPPEAKKFMEKYIKP"
BASE COUNT          711 a          398 c          446 g          587 t
ORIGIN      
        1 agccaggtaa agaatgcgac actcacgatc cagtccttca gttatatcaa cggcacagta
       61 acaaatgcaa gcgttccaat tccaggagta tatgtatcaa cgacgggcgc taattataca
      121 acagggtccg atggtaatta ttccctcagt gtacctgaag gtaattatgt tgtcaatgca
      181 atcaagcagc cggaatttta tgactatacg atctcaggtg tcgttttaaa tccatcggat
      241 acaacgataa gggacatcaa cctcataagg aaaccaaatg gaacgataag cggagttgta
      301 acgaattgat cagacccatt ggtctatcaa ttcaagaact caaataaatg aacaaatcct
      361 gacaaggaca gcagaggctc ggatttgctg gaaatcgccc ctaaatggaa tcataattat
      421 tttcttcagt tttttgtgac aaacaaattt atagtaaaag agtattacaa agaaaatgaa
      481 aatataagga tagaattagt ttgaaaaact tgtaaaatag ttcaatattt gaggtctcaa
      541 tgcctaaaaa agttctttta ataacacctc catatcactg cggagtactt gaatccgctg
      601 gctcatggat gccgctgggt atggtatatg ttgcaggaag cctgcagaat tcaggttatg
      661 atgtcaagat atacgatgca atgacaaaat tccatacttt tgatgatata aaaaaacaca
      721 taactgaata ttcaccagat gccgtaggga cggcagcata cacttcaagc ctttatgatg
      781 ctatttcggt tctcaggatc gctaaagaag taaatccatc gataatcaca atgctgggcg
      841 gtatacactc gaatttctgc tgggaggaga tccttcaaaa agatggcaac attatcgatt
      901 tcatagtacg aggagaaggt gagatcacaa caactgagct tcttaataat gtttttgcag
      961 gaaaggagcc tgatgtgccc ggtatcgcat attccgggga tggaaagatc atagctacaa
     1021 aggaaaggcc gcttataaaa gaccttgatt ctcttcccgc tgcctggaac ctgatagaat
     1081 ggaaagacta ctcatttaag acaaagaaaa actcaacatt ggcggttgta agttcttcaa
     1141 ggggatgcac gcaaaaatgc acattctgtt cccagaggct tttctggaaa gagaaatgga
     1201 ggggtcgaag ccctgaaaaa ttcgttgcag agctggaata cctgaataaa acctatgggg
     1261 tggatgttgc catgatcgct gacgaaacac ccacttttga aagggaaaga tgggagaaga
     1321 tacttgacct tctgatagaa aagaaacttg atattgaaat actgatggaa accagggttg
     1381 atgatatcct gagagataag gacataatgt ataaatattc cggtgcaggt atccttcata
     1441 tttatgttgg cgcagagtca gcgagccagg atactctaga tcgttataat aagaacctta
     1501 agatcgaaca gtccaaagaa gcaattgatt tgatcaataa tgaagggata atatctgaaa
     1561 catcatttgt cctgggaatg cctgatgaaa caccttcttc tatagataca gctattaaac
     1621 ttgcaaaata ttataatccc gatcttgcat ttttccttgc aatagcccca tggccatatt
     1681 cggatattta caaagagctt tcgccttata tagaggagtt cgattattcg aaatacaacc
     1741 ttatcgagcc catagtcaaa cctgttaaca tgacccggga ggagcttaat aaacatctgc
     1801 ttcgagcatt ccgggaattt tatatggata agctctccac catcgaagag atgagtgaat
     1861 tcaagaaaaa ttacatgatc gacgttacta gattgcttat taatgattct tacctggcaa
     1921 accagatgag aggtgaaatg cccccagaag cgaaaaagtt catggagaag tatattaagc
     1981 cgtaacttgc tttgttttat gaactgttac gaactccggt tgattttatt ctcaattgtt
     2041 ccaatatctc tttaagttta accaggaact catcctgtgg gatgtatcct ggtatccttg
     2101 agatttcttt tccgtcttca tcaaagaaga taatatatgg gg
//
LOCUS       DATGBN010000363         4836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_340558, whole genome shotgun sequence.
ACCESSION   DATGBN010000363 DATGBN010000000
VERSION     DATGBN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340558"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VKL21_08945"
     CDS             complement(<1..545)
                     /locus_tag="VKL21_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HMB45936.1"
                     /translation="MKLIFSGVVIPVSTIDWYGRSVSVIFFNGCNFKCLYCSNNNFIR
                     VANQRIEPLFKGGSVMEIEEIEKMILDARIFISAVVFSGGEPTTQYEALEHLARFAKE
                     QGLLVGIETNGYYPERLLRLIEKKLLDKIFLDIKAPLDDPQKYYYITGGRDDAAQRVR
                     KSLDLKNVEIEVRTTVFPSIS"
     gene            463..966
                     /locus_tag="VKL21_08950"
     CDS             463..966
                     /locus_tag="VKL21_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HMB45937.1"
                     /translation="MKKITDTDLPYQSIVDTGITTPLKINFINSPLSKRLSVNKPISY
                     WGVIINLAPEEINIGGKMTDMSEMFKKMGLFGIGVISLTQEKIEEFSQEMIKKGEISR
                     EEGRKFVKEVLSEQEKQMKELEDKINNRVKETLGKSGVVMKSDIASLEEKIEKLEKVI
                     QSMGKKE"
     gene            1072..2697
                     /locus_tag="VKL21_08955"
     CDS             1072..2697
                     /locus_tag="VKL21_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HMB45938.1"
                     /translation="MIKHGFGYLVDRFGLRPFRSFREKLFGPKPLKEQILILSEALRL
                     RLALEELGPTFIKFGQILSTRHDLVPKEYIEELEKLQDNVPPFELSEAKKVIEREFEE
                     KIEDIFRTFNPEQIAAASIGQVYRATLYQGEEVAVKVMRPGIETIIETDIAILMGMAR
                     FAEKHLKESKFFNPVGFVDEFSRIIRHEIDYTHEAQNAERFYSNFQGSSTVKIPRMYW
                     EYSTKHVLCQEFSEGIRISDLQKLEEAGLDKKKISTDLANAYLKMIFEDGFYHADPHP
                     GNIFVSPEGKIIFLDFGMAGHIDPALRESLTNLIISIQFNDIDVLIEALSELGLIKDA
                     GSEKPILKIKLEELLNKYYSLSSKFIDPTAFLRDLIEIFAGTKGKIPTNLMLLSKTLV
                     IRDEISRKLDPDHNFGEMTGPYVKQMLEERTKASHIIKSAQRTVMDLVNLMKNFPRKI
                     NHLLTKAEKGTLKLELEHVGLDGLVEEMDIMSNRLSFSMIIAALIVGSSLIIQTRMSP
                     SLFGVPLLGIIGFLIAGFLGLGLLISIIRSGKW"
     gene            2811..2963
                     /locus_tag="VKL21_08960"
     CDS             2811..2963
                     /locus_tag="VKL21_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45939.1"
                     /translation="MEDSTFGRRNKMNRKRKAGSASSKDPAISLDDRPVSLKQHFLYL
                     SEDLLL"
     gene            3085..4593
                     /gene="gpmI"
                     /locus_tag="VKL21_08965"
     CDS             3085..4593
                     /gene="gpmI"
                     /locus_tag="VKL21_08965"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMB45940.1"
                     /translation="MLMILDGFGLRERTQGNAIASARTPNLDHLFSSYPHSTLEASGM
                     SVGLPDGQMGNSEVGHLNIGAGRIVYQDLTRITKSIIDGDFFKNPVLLEAILNARTKN
                     SSLHLIGLLSDGGVHSHNSHLYALLELAKKNDVKKVFVHAFLDGRDVPPKSALNYIAD
                     AQIKMKESGGEFATISGRYFSMDRDKRWDRVEKAYDAMTSGIGETVSSARLAVEKAYE
                     RGETDEFVTPTVILRDNIQAPVILDNDSVIFFNFRSDRAREITRAFTIKDFKEFRRKN
                     YPNTDFVCLTQYDETFNVPVIFPSESLKNILADVLSFHHLKQLRIAETEKYAHVTFFF
                     NGGRETPVEGEERILVPSPGVATYDMQPEMSAFSVTDEAVKAISSGKFDIIILNFANP
                     DMVGHTGVFDAAVRAVEAVDKCVGILFEKVIASGGLLILTADHGNAEQMLDENGGIHT
                     AHTCDKVPFLICDPKVRVQDGILADIAPTLLEILGIEKPMEMTGKSLLVEIY"
     gene            4623..>4836
                     /locus_tag="VKL21_08970"
     CDS             4623..>4836
                     /locus_tag="VKL21_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45941.1"
                     /translation="MDSNSDLTILNGKLTILVAFGHGGELDKTQLRRFIGSEIISEKW
                     NKPIEVEQSECLTVIVAGSEKITCSTH"
BASE COUNT         1518 a          918 c         1085 g         1315 t
ORIGIN      
        1 tccgaaatcg atggaaaaac ggttgtcctg acttcaattt ctacattctt gagatccagt
       61 gactttctta cgcgttgggc tgcatcatcc cggcctccgg ttatataata gtatttttgc
      121 ggatcatcaa gcggagcttt aatatcaagg aatatcttat caaggagctt tttttctatc
      181 agccttaaaa gccgctccgg atagtacccg tttgtctcga tacctacgag caatccctgt
      241 tcttttgcga atcgggcaag atgttctaat gcttcatact gggttgttgg ttctcctcct
      301 gagaatacca cggcgcttat aaagatcctt gcatcaagga tcattttttc tatttcttct
      361 atttccatca cgctgcctcc tttaaatagg ggctctatgc gctggtttgc cacccgtatg
      421 aaattattgt tcgagcaata taaacactta aaattacagc cgttgaaaaa aatcacagat
      481 acggacctgc cgtaccagtc gatcgtggat acgggaatca ctacaccgct aaaaattaat
      541 ttcataaatt ctcctttatc aaaaagatta tcagttaata aaccaatttc atattggggc
      601 gttataataa atcttgcgcc tgaagagata aatatcggag gtaaaatgac agacatgtca
      661 gagatgttca agaaaatggg attgttcggg ataggtgtaa tttcccttac acaggaaaag
      721 atcgaggaat tttcacagga aatgataaaa aaaggggaaa ttagcaggga agaagggaga
      781 aaattcgtaa aggaagttct ttccgagcaa gaaaaacaga tgaaagagct tgaagataag
      841 ataaataatc gagtaaagga aacgcttgga aaaagcggtg tggtgatgaa aagcgatatc
      901 gcatctcttg aggagaaaat cgagaagctt gaaaaggtaa ttcagtcaat gggaaaaaaa
      961 gaataaaaat aggatcatta ctggaataag atcgattact ggaattatat cttgaacctg
     1021 cttgataaac aatacgttta cgtccgacga taccggcaaa tagtggacgt gatgatcaaa
     1081 catggtttcg ggtaccttgt agacaggttt gggcttcgtc ctttcagatc ttttcgcgag
     1141 aaacttttcg ggcctaaacc cctcaaggaa caaattctta tcctttcaga agctctgagg
     1201 ctgcgccttg cattggaaga actcgggcct accttcataa aattcgggca gatattaagc
     1261 acaaggcatg acctcgtccc taaagagtac attgaggaac tcgaaaagct gcaggacaac
     1321 gtcccaccgt ttgaattatc ggaagcaaaa aaggtgatcg agagggaatt tgaggaaaaa
     1381 atagaggata ttttccggac atttaacccg gaacaaatag ctgcggcttc aataggacag
     1441 gtatatcgcg ccactttgta ccagggcgaa gaagtggcag ttaaagtgat gagaccaggg
     1501 attgaaacta ttatagagac tgatatcgca attctaatgg gcatggcaag atttgcggaa
     1561 aagcacttaa aggaaagcaa attttttaat cctgttggtt ttgtggatga attttccagg
     1621 atcataaggc atgagatcga ttatacccat gaggcccaga atgcagaaag gttttactcc
     1681 aattttcaag gaagctccac cgtcaaaatt cccaggatgt actgggaata ttccacaaaa
     1741 catgttcttt gccaggaatt ttcagaaggc ataaggatca gcgatctcca aaaacttgag
     1801 gaggcaggac tggataagaa aaaaatatca acagaccttg cgaacgcata tttaaaaatg
     1861 atcttcgaag atggctttta tcatgcagac ccgcatccgg gaaatatttt tgtttcgcca
     1921 gaggggaaaa tcatttttct tgatttcggg atggcaggac acattgatcc tgcgcttcgc
     1981 gagagcctta caaatctcat aatatccata caattcaatg acatagatgt tcttatagaa
     2041 gcgctctcgg aactgggact aataaaggac gcaggaagcg agaagcctat tctcaaaata
     2101 aaacttgaag aactattgaa taaatattat agtctatcaa gtaaattcat agatccgact
     2161 gcattcctga gggatctcat cgaaattttt gcaggaacca aaggaaagat cccaacgaat
     2221 ttaatgctgt tgtccaaaac acttgtcata agggatgaga taagccgaaa gcttgacccg
     2281 gatcataatt ttggagaaat gacaggaccc tatgtaaaac aaatgcttga ggaaagaaca
     2341 aaagcttcac acataataaa gagcgctcaa agaacagtaa tggatctcgt taatcttatg
     2401 aaaaatttcc ctagaaagat taatcattta cttacaaaag cggaaaaggg gacactgaag
     2461 cttgaacttg aacacgtggg gcttgatggt cttgtagagg aaatggatat tatgagcaac
     2521 aggctttctt ttagcatgat aattgccgct cttatagtag gttcttcctt aattatccag
     2581 acaaggatgt cgccgagctt gtttggggtt ccacttcttg ggatcatcgg attcctgatc
     2641 gcaggatttc tgggattagg gctgctgatt tcgatcataa ggtcgggtaa atggtaatca
     2701 tcgattatcc aaatggctaa tgcatgatta tcatcacagt aattatcttc attttttaat
     2761 aatagatatc gattaatatt gcaataacaa atatagagcg atgatcatat atggaagatt
     2821 caacttttgg tcggcgaaat aaaatgaaca ggaaaaggaa agcaggctca gcttcttcaa
     2881 aagatcccgc aatatctctt gatgaccgcc ctgtatctct gaaacaacat tttctttatt
     2941 tatctgaaga tttgctgctt tgatttatat tatttttttt tgtctgtgtc ctctgtggtt
     3001 gattatcttt gccaaagatt atatcccatg tcatcataac agcatcttat tctattagcg
     3061 aaaatgatcc gaaaaagacc tgttatgctg atgattctcg atggctttgg ccttcgcgag
     3121 agaacgcaag gaaatgctat cgcatcagcg agaacgccaa accttgatca tttattttcg
     3181 agttatcccc actcaactct tgaagcatcg ggcatgtctg tcgggcttcc cgatggccag
     3241 atggggaatt ctgaagtagg ccatctgaat atcggagcag gaaggatcgt ttaccaggac
     3301 ctgacaagaa ttacaaaatc aataatcgat ggtgattttt tcaagaatcc ggttcttctg
     3361 gaggcgattt tgaacgccag aacaaaaaat tcgtcgctgc atctcattgg attgctttca
     3421 gatggcgggg ttcatagtca taacagtcat ctgtatgctc tccttgaact tgcaaaaaaa
     3481 aatgatgtaa agaaagtgtt cgtacatgca tttcttgacg gcagggatgt accacccaaa
     3541 agcgctctta attatattgc agatgcccaa atcaaaatga aagaatcagg aggggaattt
     3601 gccaccattt caggcagata tttttcaatg gatagggaca aaagatggga cagggtcgaa
     3661 aaagcatatg atgccatgac ctcagggatc ggcgagacag tgagctcggc ccgccttgct
     3721 gtagagaaag catacgagcg cggagaaacc gatgagttcg taactccgac ggtgatctta
     3781 agagataata ttcaggcccc tgtgatcctg gacaatgact cagtgatttt cttcaatttt
     3841 cgctcggacc gcgcgcggga gataacgagg gcctttacga tcaaagactt caaagagttc
     3901 aggcgaaaaa attacccgaa tacggatttc gtatgcctga cacagtatga tgagactttt
     3961 aatgttccgg tcatatttcc atccgaatcc ctgaaaaata tacttgctga tgtgttatcc
     4021 tttcaccact taaaacagct tcgcatagca gagaccgaaa aatatgcgca tgtaacattc
     4081 ttttttaatg gcgggcggga aacacctgtt gaaggtgaag aaaggatact tgtgccatcg
     4141 cccggagttg cgacttacga tatgcagccg gaaatgagcg ctttttccgt cacggatgaa
     4201 gctgtgaaag cgatatcatc cggcaagttt gacattatta tcctgaattt tgcaaatcct
     4261 gatatggtgg ggcacacagg cgtttttgat gctgccgtta gggccgtaga agcggtcgat
     4321 aaatgcgtgg gaatattgtt cgaaaaagtg atagcatcag gcggactgtt gatattgacg
     4381 gcagatcatg gcaatgccga gcagatgctt gatgagaatg gcgggataca tacagcccac
     4441 acatgcgata aagtgccttt ccttatctgt gacccgaaag tcagggtgca ggatggaata
     4501 cttgcggata tagccccgac gcttcttgaa atcctgggga ttgaaaaacc aatggagatg
     4561 accgggaaat ctcttttggt ggaaatttat taatactttt taagcatatt attcatatat
     4621 atatggattc aaacagcgat ttgaccatcc tgaatggcaa actcactatt cttgtggctt
     4681 tcggacatgg gggagaactg gataaaacac agttaaggag attcattggt tcggaaatca
     4741 tttcggaaaa atggaataaa cccatagagg tggaacaatc agaatgcctt actgtcatag
     4801 tggcgggcag cgaaaaaata acatgcagca cgcatg
//
LOCUS       DATGBN010000364         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_146443, whole genome shotgun sequence.
ACCESSION   DATGBN010000364 DATGBN010000000
VERSION     DATGBN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146443"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..813
                     /locus_tag="VKL21_08975"
     CDS             22..813
                     /locus_tag="VKL21_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB45942.1"
                     /translation="MPLYPIVLLDHISFIRNGTRILEDITLRIDHGQNWAIIGQNGSG
                     KTSLISIIGGYHHPSEGKAIVLGKKFGSTDLRELRLRIGECSSEIRDIIHNHETVKDI
                     VLSGRFASIGLYETPASKDHERAAYLLDFLGLSDKSDRRFMTLSDGEKQKVLLARALM
                     PQLELLVLDEPCAGLDLKAREEFLDAVQEMCTKKGGPALIYITNHIEEIIPAITHALT
                     LRKGMVIASGRKEDVLTNEVLSETFEVPIELHEKEGRIWPMILKR"
     gene            1138..2007
                     /locus_tag="VKL21_08980"
     CDS             1138..2007
                     /locus_tag="VKL21_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HMB45943.1"
                     /translation="MTTDPSHIARCAQLAMILEVSATPKPGNIDRDHNYSDTRFEHFL
                     ASAVGVYPILEKAARSDSGVGELIDRSVIECSKWQRGGNTHFGAFILLIPLVMAGGRC
                     EKITCLKEQVQKIVKETTIYDAVEFYKAFTRARVKVKPVDDLDFSDTDSIGKIKAQGL
                     TLFNMMEISGSYDMIAGEWINGFDKTFECSALIQDKIRKYGINDAVVLAFMELLSRNN
                     DTFISTKFNKKKAEEVSMRARNILLKGDMDRIRNEIRQFDEELLNEGVNPGSTADIII
                     AGLFVSLFEGMRF"
     gene            2007..2573
                     /locus_tag="VKL21_08985"
     CDS             2007..2573
                     /locus_tag="VKL21_08985"
                     /inference="COORDINATES: protein motif:HMM:NF016199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HMB45944.1"
                     /translation="MDIEEFGIHEGISEVIFSTISPEGIPNAAPIGLHLENGKFFARI
                     YNSNTLDNILGNQTAAANIVDDPFLFVQSALSDIEPEKFEFIEGFPVLKDALGWIVFD
                     CRCRKGENISVVELSPARSKISKRKIVPVNRGFYAVIEATVHATRYVVLKEQQYLDHI
                     NYYNTIVQKCGSTGDKDAMKLLYDLIRS"
     gene            complement(2785..3771)
                     /gene="ruvB"
                     /locus_tag="VKL21_08990"
     CDS             complement(2785..3771)
                     /gene="ruvB"
                     /locus_tag="VKL21_08990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMB45945.1"
                     /translation="MEERIITPISQNEEKEELTIRPARLDDFIGQHALKESLKIFIEA
                     SKKRNEPLDHILFSGPPGLGKTTLAHIIARELGSNIKSTSGPVLEKPGDLAAILTALK
                     KGDVLFIDEIHRLNSVIEEILYPAMEDYEIDVMIGEGPGARSIKLTLERFTLIGATTR
                     VGLLGSPFRDRFGLISRLNLYSVDELVEVVSRSASILKIPITGDGSIEIAKRSRGTPR
                     IANRLLRRVWDFALVRAQGNITHDVADAALTMLGIDRLGLDELDRKILGIISEDFGGG
                     PVGAKTIAISAGEEVRTIEEVYEPYLIQIGFIKRTPHGRETTAAAKEHLTKK"
BASE COUNT         1081 a          810 c          900 g         1027 t
ORIGIN      
        1 taacatgatg ataaaaacat tatgccctta tatccgattg ttttgcttga tcatatttca
       61 ttcattcgaa atggcacaag aatcctggaa gatattaccc ttcgcataga tcatggccag
      121 aactgggcta taatcggtca aaacggatcc ggcaaaacca gtcttataag tataatcggt
      181 ggttatcacc atccatcgga aggaaaagcc attgtcctgg gaaaaaaatt cggctccaca
      241 gacctcaggg aactccgctt gcggatagga gaatgcagtt ccgagatacg ggatattatc
      301 cataaccatg aaacagtaaa ggatatagta ctttcgggaa ggtttgcaag tatcggcctc
      361 tatgaaacac cagcttcaaa ggatcatgag cgtgcggctt atttacttga tttcctgggg
      421 ttatccgata aaagcgaccg ccgtttcatg accctttctg atggtgaaaa acagaaagtc
      481 cttcttgcaa gggcgctgat gccccaactg gagttgctag ttctggatga gccatgtgcg
      541 ggacttgacc tgaaagccag ggaagaattc ctggatgctg tgcaggaaat gtgtacgaaa
      601 aaaggaggtc cggctttgat ttatattaca aatcatatcg aggagatcat tcctgcaata
      661 acacatgccc tgactttacg aaagggcatg gtgatagcaa gcggcaggaa agaagatgtg
      721 cttaccaatg aagttctaag tgagactttt gaagtaccta tcgaattgca tgagaaagaa
      781 gggcggatat ggcccatgat attgaaacgt taaaactttt ttttagctgg tgagggcgcg
      841 ggattttcga aggatattgg acaaataaca ggattgttat ataacaaatt tgttatatga
      901 tgaatatcct ggccagcaac aggacttgtt cttgcatgaa tttaagtaat ctaaaataat
      961 attatgttga atgcttccga aaaagatcct cacgcatgga aaggaacaac atagaacacg
     1021 gatgacgcgg attgggcgga ttttcacgga tatgcgtgca tctgtgttct ttcgcgatct
     1081 atctaagttc attatgttta aaccctgccg gttctcaact cctatagagc aaatattatg
     1141 actaccgatc catcccacat cgcccgatgc gcccagctgg ccatgatcct tgaggtctca
     1201 gcaaccccca aaccgggcaa tatcgaccgc gatcacaact attcggacac gcgcttcgaa
     1261 catttcctgg caagcgctgt cggggtatat ccgatacttg agaaagccgc gcgctcggat
     1321 tcaggggtcg gagaactcat tgacaggtca gtgattgaat gcagcaagtg gcaacgaggc
     1381 ggtaacaccc atttcggggc atttatcctg ctaataccgc ttgttatggc aggaggcagg
     1441 tgcgagaaaa ttacctgcct gaaagaacag gttcaaaaga tcgtaaagga aacaacgatt
     1501 tatgacgcag ttgaatttta caaggctttt accagggcca gggtgaaggt caaacctgtt
     1561 gatgaccttg atttttcgga cacagattcc attggaaaaa taaaagcaca gggattgacc
     1621 ctttttaaca tgatggagat atcaggaagc tatgatatga tcgcaggtga atggataaat
     1681 ggttttgata aaacatttga atgcagcgcc ctgatacagg ataagatcag aaaatacggg
     1741 ataaatgatg ctgtggtgct cgcattcatg gagcttttat ccaggaacaa tgacacattc
     1801 atctctacaa aattcaataa aaaaaaggca gaagaagttt caatgcgagc aagaaatatt
     1861 ttactaaaag gcgatatgga caggataagg aatgaaatcc ggcaatttga cgaagagcta
     1921 ttgaatgaag gtgttaatcc gggatcgaca gctgatatta tcattgcagg attgttcgtt
     1981 tcgctttttg aggggatgag attttgatgg atatcgaaga attcgggatt catgagggaa
     2041 tatcggaagt gattttctca acaatatccc ctgaaggcat tccaaatgct gcgcctattg
     2101 gtctgcatct tgaaaacgga aagttttttg ccaggatata caactcaaat accctggata
     2161 acatattggg caatcagact gctgcggcaa atatcgttga tgatccgttt ttatttgtgc
     2221 agtctgcact ttcggatatc gaaccggaaa aattcgaatt catagaaggg ttcccggttc
     2281 tgaaagacgc acttgggtgg atcgtgttcg attgcaggtg caggaaaggt gagaatattt
     2341 cagtcgttga gctttcacca gctagatcaa aaatctcaaa gcgaaagata gtgcctgtaa
     2401 atcgcggttt ttatgcggtt atcgaggcaa cggttcatgc cactcgctat gtggttctga
     2461 aggaacaaca atacctggat cacatcaatt actacaatac gatagtccag aaatgcggca
     2521 gtactggcga caaggatgcg atgaagctgc tttatgattt gatccggtcg taagcaacag
     2581 ataagcgtat tattaacact ttcggtcaac attatcttaa cggcgctggg gtgcgacccc
     2641 agaccccaac ggatgcgccg ccgctggcgg tgtttaacgt gaaatcactg gttgcataat
     2701 attcaatcat ccatacatgc atgtaattaa tgaaaaaatt atctcgatat cgtttgacaa
     2761 tttagccttg ataggaaatc tgtactattt tttggtcaga tgttctttgg ctgccgctgt
     2821 tgtttccctt ccatgcggcg tacgtttgat aaatcctatc tggataagat aaggctcata
     2881 aacttcttca atcgtcctga cctcttcacc tgctgatatt gcgatcgttt tagccccgac
     2941 aggccctccg ccgaaatctt ccgaaattat tcccaggatc ttcctgtcaa gttcatcaag
     3001 cccaagcctg tcgatcccaa gcatggtcag ggcagcatcc gcaacatcat gcgtgatatt
     3061 gccctgtgcc ctcacaagcg caaaatccca tacgcgccga agcagcctgt tcgctatgcg
     3121 gggagttcca cggcttcttt ttgcgatctc aatggaaccg tcccctgtta tggggatttt
     3181 cagtatggag gcgcttcggg aaacaacctc aacaagctca tccacagaat aaaggtttaa
     3241 cctggaaata agaccaaacc tgtcacgaaa tggcgaacca aggagtccca ccctggtcgt
     3301 ggcccctatg agagtaaatc gttcaagggt caattttatg gagcgagcgc ctggtccctc
     3361 gcctatcatg acatctatct cgtagtcctc catcgcagga tataatattt cctcaataac
     3421 ggaattcagg cggtgtatct catcaatgaa cagcacgtcc cctttcttta atgccgtgag
     3481 gatggctgca aggtctccgg gtttttccag aactggcccg gaagtgcttt tgatattgga
     3541 gcccaattcg cgggcaatta tatgggccag ggtggtcttg ccaaggccgg gaggccctga
     3601 aaacaggata tgatcaaggg gttcatttcg tttcttcgat gcttctatga aaattttaag
     3661 ggactccttg agggcatgct ggcctatgaa atcatcaagt cgagcggggc ggatggtgag
     3721 ctcttctttt tcttcatttt gcgagatcgg ggtgattatt cgttcttcca tatcattcct
     3781 tcaatttcaa aagcgctgcc ttgattatgg cttccaca
//
LOCUS       DATGBN010000365         8757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_495604, whole genome shotgun sequence.
ACCESSION   DATGBN010000365 DATGBN010000000
VERSION     DATGBN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495604"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="fabG"
                     /locus_tag="VKL21_08995"
     CDS             <1..743
                     /gene="fabG"
                     /locus_tag="VKL21_08995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMB45946.1"
                     /translation="INYQNGKEHAQTVSKLINRMGMAEDIEEITEKIEKMEDKEHAKE
                     ISDLIESIGCHSCVCQANVSDFEQVKKMQEDVIKKFGKVDILVNNAGIVRDKSFVKMT
                     SDMWNDVLSVNLDGTFYCTKAFVDGMLERKYGRILNISSVIGRMGNFGQTNYAASKAG
                     MIGLTKALAKEFAGKGITVNAIAPGFIETDMLKGLSPEAKKKIVAKIPLGRLGKASEV
                     ASTVAFLASQEGDYITGQVIDINGGLYI"
     gene            complement(850..1758)
                     /locus_tag="VKL21_09000"
     CDS             complement(850..1758)
                     /locus_tag="VKL21_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB45947.1"
                     /translation="MKEINQSNNNKKINFFITSDTFPSISVTGNSCSLLCKHCQGKLL
                     QRLIHATTNEELEKKALLLHRKGAKGILLTGGCDERGCVPIQNLTPAIKKLKEETDLI
                     LIAHTGFISGEEAGLLKNSGLDGIGFDVMGDMTSVLEVYGLRVSEKEYIDSLHAISQS
                     GLFIFPHVCVGIHFGKLRGEYRALEMIRTIAPTAVIITGLMPVAGTPMENIKPDPSDF
                     ENVIKKAIRMFPHTPVLLGCAHSSGKDRADIEKMALLCGVSGIAAPALNTIEFAEENG
                     YEMKFYGTCCGLIPDEKMKIRNPSES"
     gene            1816..3111
                     /locus_tag="VKL21_09005"
     CDS             1816..3111
                     /locus_tag="VKL21_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopropylmalate synthase"
                     /protein_id="HMB45948.1"
                     /translation="MKIYRSYEDLPKIKLPLGQDIFISDSTIRDGSQMPGIVMKKRHK
                     TEIYEYLHKIGIEKLETFVYNERDREAVNEMLDRGYEFPEVTGWARANPKDIDFVLNM
                     DGIKETGILMSVSDSHVIDKMGLKSREEAEKKYTDALQYAVDHGLRTRAHIEDMTRAD
                     NEGFVFPLIKKLLEIDPDCIIRLCDTVGFGLPFPGVDEPYGIPHITQRLLEIGTKNIE
                     THIHDDFGMSVACSIAGYWYGANWSSLTFLGIGERAGNAELEKVVLFLIQRVEGFDKY
                     DLKSVTEFARFMEREIGIRVPRNKSVVGKNIFAHESGIHTAGVIKNPFTYEPYPPDIV
                     GGKRQLLIGDSSGIEVLRFKVEESLNELMHIDTNIEKNDLRLKAIQADIQKLYNQEKR
                     VSCISDEELKKYVEKYFMLESIIDEHSTDEDNVDNRNNK"
     gene            3118..3468
                     /locus_tag="VKL21_09010"
     CDS             3118..3468
                     /locus_tag="VKL21_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45949.1"
                     /translation="MDIKKTAIVLNGFIHDFSTGYWVASMITIILLHDFQAKYPGVSD
                     LLMVIEQFFFWNTIGAVIVIFATGGIRTFTYVDNYYGKEIEDSRRKLLVIKHIVLFAI
                     FGISGYFAYLMAFQ"
     gene            3650..6514
                     /gene="gyrB"
                     /locus_tag="VKL21_09015"
     CDS             3650..6514
                     /gene="gyrB"
                     /locus_tag="VKL21_09015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMB45950.1"
                     /translation="MNEVNKEYGAEQIQVLEGLEAVRKRPSMYIGSTDARGLHHIVYE
                     VVDNSIDEALAGYCTNIEIMIRPDHSVRVMDNGRGIPVEVIPKYQKSALEVVLTMLHA
                     GGKFDNNAYKVSGGLHGVGVSAVNALSEWMEVEVKRGGKLYKQRYEYGKPVTPVTTIG
                     ESTETGTIITFKPDKTIFETLEFNLETLANRLRELAFLNKGVRISLKDEFTQNEQVFQ
                     YEGGIVSFVEFLNKNKNLLHEKPIYFQKQKDTTVVEIAMQYNDSYTENVYPFANNIHT
                     HEGGTHLVGFKAALTKVSNDYAKSKNFLKGDDKLSGEDVREGLTAIINVKLTNPQFEG
                     QTKTKLGNSDIKGIVETLVSEGLSEFLEENPAAAKNILTKALEAAEAREAARKARELT
                     RRKSALEVGSLPGKLADCSEKDPAKSEIYIVEGDSAGGCFSGDTLVALADGRALSFTE
                     IIAEQADGKEHFCYTIRNDGKIGLERVINPRKTKTNAKIIKVTFDNGETITCTPDHPF
                     MMSDGSFKRIDKVTPNDSLMPLHRKSCDIKEPGIKIACEAVSMFNHRIVSIESLEETM
                     DVYDIEVPNSHNFALESGVFVHNSAKQGRDRKFQAILPLRGKILNVEKARLTKILKNE
                     EIRALITVIGAGIGEGEEFDIQKARYHRIIIMTDADVDGSHIRTLLLTLFYRYMRQLI
                     DLGYIYIAQPPLFKVKKGKSEDYVYNEEELDKWFSNYTSENVTLYSKTDKKEYTKEEI
                     TNLLNYLIKYKNYYEKYRKMGLPQSFVDTFIKKKDLLMGKISESDIEGREKLFSIPNI
                     KKIIIEKSEDRTWSVKAEGDFDDKTKMSLENLIEDPEFSEFYETHKKIESLGKPPFVI
                     VESETKEQEVSTTFDLATILLEYGRKGITLQRYKGLGEMNPQQLWDTTMNPDTRTMLK
                     VTLEDAIKEDAIFTILMGDKVEPRREFIERHAKDVRNLDV"
     gene            6523..6840
                     /locus_tag="VKL21_09020"
     CDS             6523..6840
                     /locus_tag="VKL21_09020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB45951.1"
                     /translation="MRREIFDRMKNMSQRLKDKYDAQTIILFGSYARGDETDDSDIDL
                     FVIAPRRERFFERIASVKCLIRDLRNGLPVAPIVLTPEEIEKRMKLGDQFIMDILENG
                     VRL"
     gene            6837..7115
                     /locus_tag="VKL21_09025"
     CDS             6837..7115
                     /locus_tag="VKL21_09025"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMB45952.1"
                     /translation="MNEKEGLLSSEWISIARKDWNRVYHMLQYDDFEASGLFLQQSLE
                     KYLKAFLLQQGWKLRKIHELDALLDEALMNLSYRNKILDEAFSEQIEK"
     gene            7127..7843
                     /locus_tag="VKL21_09030"
     CDS             7127..7843
                     /locus_tag="VKL21_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMB45953.1"
                     /translation="MKSSAKRKNPEKWMAEGLALNNLKKYKEAIESFDRILKIDPKDA
                     GALFNKGRALHNLDDLTNAITCYNRVLQLNPKNAKAWRWKGFALRSLERFEEAVKCFD
                     KEIELEPNNDYIWYIRYNQGLMLHVLDRFDEAIKSYDASIGSFPEYAEAWYCRGLAYY
                     NMEKYDEVIKSCDKTLEIDPDYFYAWYGKGLALHSLNNYQEAIKCYEKVLENDPVHID
                     AWFNKKMATRCLGLFNQSLS"
     gene            7851..8171
                     /locus_tag="VKL21_09035"
     CDS             7851..8171
                     /locus_tag="VKL21_09035"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB45954.1"
                     /translation="MQRITEQLIEEIRDRIVSAVQPEKIILFGSYAYGTPTKDSDLDL
                     LVIMPSYEPMHKRIFRIRKILRDFRVPKDVIVYTPQEVNDWKDVSNAFITSIMRKGKV
                     IYGL"
     gene            8161..8442
                     /locus_tag="VKL21_09040"
     CDS             8161..8442
                     /locus_tag="VKL21_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45955.1"
                     /translation="MDYKDLAIKWFNKANNDLIAGNYILTMPDPPADTICFHSQQSVE
                     KYPSELSSYAVDVRYPMEGNYDVTIEEAKRAIDIAGKIKIFVLNKLELS"
BASE COUNT         2862 a         1572 c         2004 g         2319 t
ORIGIN      
        1 ttatcaacta ccagaatgga aaagaacatg cccaaacggt ttcaaaactg attaaccgca
       61 tgggtatggc agaagatatt gaagagataa ctgaaaagat agagaaaatg gaagataagg
      121 aacatgcaaa ggaaatatcc gatctgatag aatcgatagg atgtcattcc tgcgtctgcc
      181 aggccaacgt gagtgacttt gagcaggtaa agaagatgca ggaagatgtt attaagaaat
      241 tcgggaaggt cgatatcctt gttaataatg cagggatagt gagggataaa tctttcgtga
      301 aaatgacatc tgatatgtgg aatgatgtct tatcagtgaa tcttgacggg acgttttatt
      361 gcacgaaagc ctttgttgat ggaatgcttg agagaaagta tgggcggata ttgaatattt
      421 cctcggtcat aggcaggatg ggtaatttcg gccagacaaa ttatgcggca tccaaagccg
      481 gtatgatcgg tctcacaaag gcgcttgcaa aggaatttgc cggaaagggt atcactgtga
      541 acgcaatagc gcccggattc attgaaaccg atatgttaaa aggtctctct cccgaagcaa
      601 aaaaaaagat cgtggctaag atcccgctgg gaagattggg aaaggcatcg gaagtcgcat
      661 ccactgtggc attcctggca tcgcaggaag gggattacat aacaggacag gttattgaca
      721 taaatggggg gttgtatatt taggtcaatt actcccgcct agttatttga aggcggggta
      781 ttctctacca gttgacccct gataataaaa gcctgttccc ggatgcattg agacatcagg
      841 tcatgaaaat taagattctg atggatttct tatcttcatt ttttcatccg gaattaaccc
      901 gcagcatgtg ccataaaatt tcatctcata accgttttct tctgcaaact caatagtatt
      961 caaagccgga gccgcaatac ctgacacacc gcaaagaagc gccatctttt caatatctgc
     1021 cctatctttc ccgcttgaat gggcgcaccc cagaaggacc ggagtatggg ggaacatccg
     1081 gatcgccttt tttattacat tttcaaaatc ggatgggtct ggcttaatat tctccatcgg
     1141 cgtcccggcg acgggcatta atccggttat tattaccgct gtgggcgcta tggttcggat
     1201 catttcaagg gcgcggtatt cacctcttaa tttgccaaaa tgtattccta cacaaacatg
     1261 gggaaatatg aatagtcccg attgagaaat tgcgtgaagg ctgtctatat attctttctc
     1321 ggaaactcga agaccatata cttccaatac ggaagtcata tcgcccatta catcaaaacc
     1381 gattccgtca agccctgagt tttttaataa tcccgcttct tccccggata taaaacccgt
     1441 atgcgcaata aggataaggt cagtctcttc cttgagcttt tttattgcag gtgttaaatt
     1501 ttgtatgggc acgcacccgc gctcatcgca accgcctgtc agaagtattc cttttgcgcc
     1561 cttcctgtga agcaatagcg ctttcttttc aagctcctcg tttgtggttg cgtggatcag
     1621 tcgctgcagc aatttgccct ggcaatgctt gcacaaaagg gaacatgaat ttcctgtgac
     1681 ggaaatggaa gggaacgtgt ccgatgtgat gaaaaaattg atctttttat tattattaga
     1741 ctgatttatt tctttcatat gtggtatata ggaaaaccac aagtaatata aatactgata
     1801 acaatcaatc agccaatgaa aatctacagg tcatatgagg atttgccaaa gatcaaactg
     1861 cctctggggc aggacatctt tataagcgac agtaccatac gcgacggttc gcaaatgccc
     1921 ggaatagtga tgaaaaaacg gcataagacc gaaatttatg aatatctgca caagatcggc
     1981 atcgagaagc ttgaaacttt tgtctataat gagcgcgacc gggaagctgt gaacgaaatg
     2041 cttgacaggg gatatgagtt ccctgaggtg acaggatggg caagggcaaa tcccaaggac
     2101 attgattttg ttctcaatat ggacggcatt aaggaaacgg gcatattgat gtctgtttca
     2161 gactcccatg taattgacaa gatggggctt aagagcaggg aagaggctga aaagaaatat
     2221 actgatgcat tacaatatgc cgttgatcac ggcctcagga caagagcgca tatagaagac
     2281 atgacgcgag cggataacga aggtttcgtg ttcccgctta ttaaaaagct tcttgagata
     2341 gatccggatt gcattattcg tctgtgcgat accgttggct ttgggttgcc atttccaggg
     2401 gttgatgagc catacgggat cccccacatt acacagcgcc ttctggagat cggaacaaag
     2461 aatatcgaaa cccatataca tgatgatttt gggatgtcgg tggcgtgttc aattgcagga
     2521 tactggtatg gcgccaactg gtcaagcctt accttcctcg gaatcggaga gcgagccggg
     2581 aacgcagagc ttgaaaaagt agttctattt ttgatacaga gagtagaggg atttgataaa
     2641 tacgacctta aatccgtaac tgaatttgcg cgattcatgg aaagagagat cgggatacgc
     2701 gtcccgcgca acaaatcggt tgtcgggaag aatatttttg cccatgaaag cggcatacat
     2761 acggccgggg tcattaaaaa tccattcacg tacgaacctt atccacctga tatcgtaggc
     2821 ggaaagaggc agttattgat aggtgattca tcgggaatcg aagttttgcg atttaaagta
     2881 gaagaatccc tgaatgaatt gatgcacatt gacacaaata ttgagaaaaa tgaccttcgt
     2941 ctcaaggcga tccaggctga tatccagaaa ttgtacaacc aggagaaacg tgtttcgtgc
     3001 atttctgatg aagagctcaa aaaatatgtt gagaaatatt tcatgctcga atcgatcatt
     3061 gatgaacata gtaccgatga agataatgta gataatcgaa ataataaatg aaatgtcatg
     3121 gatattaaga aaaccgcgat agtcctgaat ggatttattc atgatttttc tacaggttat
     3181 tgggtagcgt cgatgataac catcatttta ttgcatgatt tccaggcgaa atatccggga
     3241 gtatccgacc tgcttatggt gatagaacag ttcttcttct ggaatacaat tggcgctgtt
     3301 atagtaattt ttgcaactgg cgggatacgg acttttacat acgtggataa ttattacgga
     3361 aaagagatcg aagattcgcg aagaaaattg cttgtaataa aacacattgt tttgttcgca
     3421 atattcggaa ttagcggtta ttttgcctat ttaatggcat ttcaataaat taatgaccaa
     3481 aaaatggtaa aatcgctatg caatgaaggt aataaggtat atatgctata aaaacaatca
     3541 gaccccgaag gtattcaaat actgaaggca tgaaatttaa tttggaatta gttgaaaacg
     3601 gaaaaatttt aaataaaata ttggtataac tattaaaaag gtactcgata tgaacgaagt
     3661 caataaagaa tatggggcag aacagattca ggtgcttgag ggacttgaag ctgtacgcaa
     3721 acgccccagc atgtatatag gcagtacgga cgcacgcggg cttcaccaca ttgtatatga
     3781 agttgtggat aacagcatag atgaggcgct tgctggttac tgcacgaata tcgaaataat
     3841 gatccggcca gaccacagtg tcagggtaat ggacaatgga agaggtatcc cggtcgaagt
     3901 tatccctaaa taccagaaat cggctcttga agttgttttg actatgctcc acgcaggtgg
     3961 aaaatttgac aacaacgcat acaaagtctc tggcggcctt catggtgttg gcgtgtctgc
     4021 ggtcaatgct ctttccgaat ggatggaagt tgaagtcaaa agaggaggca agctctataa
     4081 gcagcgatat gaatatggaa agcccgttac tcccgtgaca acaataggcg agtcaacaga
     4141 gacagggacg atcatcacat ttaaaccaga caagactatt ttcgaaacgc ttgaattcaa
     4201 tcttgaaact ctggcaaaca ggttgcgtga acttgcgttc ctcaataagg gagtaagaat
     4261 atccctcaaa gatgaattca cacagaatga acaggttttc cagtatgagg gcgggattgt
     4321 ttcattcgtt gagttcctga acaagaacaa gaacctgttg catgaaaaac caatttattt
     4381 ccagaaacag aaagacacta ctgttgtgga gatcgcaatg cagtacaacg acagctatac
     4441 tgagaacgtc tatccttttg caaataacat tcatacgcat gaaggcggta cgcatcttgt
     4501 ggggtttaaa gccgcgctca ccaaggtatc aaacgattat gcaaaatcaa agaattttct
     4561 aaaaggggat gacaaactct ctggtgagga tgtaagggaa gggctcactg cgataattaa
     4621 tgtcaaactg acaaacccgc agtttgaagg acaaacaaag acaaaacttg gtaatagcga
     4681 cataaagggt attgttgaga cgcttgtttc cgaaggtctt tcggagttcc ttgaagagaa
     4741 cccggccgca gcaaaaaaca ttctaacaaa ggctcttgaa gcagccgaag caagggaagc
     4801 agccagaaaa gcgcgtgaac tgaccaggcg aaaaagcgct cttgaagtag gctcgcttcc
     4861 tggaaaactt gcagattgct cggaaaaaga ccctgcaaag agcgagatat atatcgtgga
     4921 aggggattcg gcgggcggtt gtttttcagg agatacgctt gttgcccttg ctgatggaag
     4981 ggctctaagt ttcacagaaa tcatagcaga gcaggctgat ggaaaagagc atttttgcta
     5041 cactattcga aatgacggca aaatcgggtt agagcgagta attaatcccc ggaagacaaa
     5101 aaccaatgca aaaatcatca aagttacatt cgataatggc gaaacgatca cgtgtactcc
     5161 agatcatccg ttcatgatga gtgatggtag tttcaaacga attgataaag tcactccgaa
     5221 tgattcgtta atgcctcttc atcgaaaatc ctgcgacatc aaagaaccag gaataaaaat
     5281 agcatgcgaa gccgtcagta tgtttaacca tcgtattgta tctattgaat ctcttgaaga
     5341 aacaatggat gtttacgaca tagaagttcc aaattcacat aacttcgctc ttgaaagtgg
     5401 cgtttttgtt cataatagcg ctaagcaggg acgcgatcgc aaatttcagg cgatcctgcc
     5461 tcttcgcgga aagatattga atgtggaaaa agcgcgcctg acaaagatat tgaagaacga
     5521 ggaaatacgc gcgcttatca cggtcatagg ggcaggtatc ggtgagggtg aggaattcga
     5581 tatccagaag gcacggtatc atcgtataat aattatgaca gatgctgatg ttgatgggtc
     5641 gcacatccga acattattgt taacgttgtt ctacaggtac atgcgccagc tcatcgatct
     5701 gggttatatt tacattgccc agcccccgct ttttaaggtg aagaaaggaa aatctgaaga
     5761 ttatgtgtac aacgaggaag aattagataa atggttcagt aattatactt cagaaaatgt
     5821 tactttgtat tcaaaaacgg ataagaaaga atatacaaag gaagaaataa caaatttatt
     5881 gaattatctt attaaataca aaaattatta tgagaaatat cgaaagatgg gattacctca
     5941 aagttttgtc gatactttca ttaaaaagaa agatctttta atgggtaaga tatctgaatc
     6001 tgatattgaa ggaagagaaa aactttttag tattccgaat ataaaaaaaa ttatcattga
     6061 aaaaagtgaa gacaggactt ggtcggtaaa agctgagggt gattttgatg ataagacaaa
     6121 aatgtcctta gagaacttga ttgaagatcc agaatttagt gaattttatg agactcataa
     6181 aaaaattgaa tctttgggaa aaccgccttt tgtaattgta gagtctgaaa caaaagaaca
     6241 agaggtttca acaacatttg atttagctac tattttatta gaatatggta gaaaaggcat
     6301 aactttacag cgatacaaag gtttaggtga gatgaatccg cagcagctct gggataccac
     6361 aatgaatcct gatacaagga cgatgctcaa agtcactctt gaggatgcca tcaaggagga
     6421 tgcgatattt accatactta tgggtgataa ggtggagccg cggcgtgaat ttatcgagag
     6481 gcatgccaag gatgtaagga acctggatgt atgagggagg gtatgagaag agagattttt
     6541 gacaggatga aaaatatgag ccaaaggctg aaggataaat atgatgcgca aacgatcatt
     6601 ctatttggct cctatgcaag aggagatgaa acagatgaca gcgatattga tttattcgta
     6661 atagcgccgc ggcgggaaag attcttcgaa agaatagcgt ccgttaaatg tcttatacgc
     6721 gacctgagaa atggattgcc tgttgctcct attgtcctta cacctgaaga aatcgaaaag
     6781 agaatgaagc ttggcgacca gttcatcatg gacatactgg aaaatggagt gcgtttatga
     6841 atgaaaaaga ggggttgtta tcttctgaat ggatcagcat tgcaagaaaa gactggaacc
     6901 gtgtatatca tatgctacaa tatgacgatt ttgaagctag cggtcttttt cttcaacaat
     6961 cattagaaaa atatttaaaa gcttttcttt tgcagcaggg ctggaaacta cgaaaaattc
     7021 atgaacttga tgctttactt gatgaagcac taatgaattt atcatatcga aataagattc
     7081 tggatgaggc tttttctgaa caaattgaaa aatagaggag agaattatga aatcatcagc
     7141 aaaaagaaaa aaccctgaaa agtggatggc agaaggtctc gccttaaaca atttaaaaaa
     7201 atataaagaa gcgatagaat cttttgacag gatactcaag atcgacccga aggatgcagg
     7261 cgccctgttc aataagggac gcgcgcttca taatctggat gatcttacta atgcaataac
     7321 atgttataac agggtcctgc agttaaatcc aaaaaatgca aaagcatggc gctggaaggg
     7381 atttgcactt cgaagtcttg agcgctttga agaggctgta aagtgctttg ataaagagat
     7441 tgagcttgaa ccaaacaatg attatatctg gtatatacga tacaaccagg gacttatgct
     7501 tcatgttctg gacagatttg atgaagccat caaaagctac gatgcttcga taggatcatt
     7561 tccggaatat gcggaagctt ggtactgcag gggacttgct tattataata tggaaaaata
     7621 tgacgaagtc ataaaaagct gtgacaaaac ccttgagatc gatccggatt atttctatgc
     7681 atggtatggc aaagggcttg cgcttcatag tttgaataac tatcaagagg ctataaagtg
     7741 ttatgaaaag gttttagaga atgaccctgt acatattgat gcatggttca ataagaaaat
     7801 ggcgactcgc tgtttaggac tttttaatca aagcttatca tgaaaaaatt atgcaacgaa
     7861 taacagaaca actcattgag gaaattagag atcgcatcgt tagtgcagtc caacctgaaa
     7921 agataatact tttcggatcg tatgcctatg gaactccgac aaaggacagc gatctggatt
     7981 tattggtgat aatgccatcg tatgaaccga tgcataagag gatatttcga atcaggaaga
     8041 tacttcgcga ttttcgagtt cccaaagatg ttattgttta tacaccacag gaagtcaatg
     8101 attggaaaga cgtatcaaat gcgttcataa catcaataat gagaaaagga aaagtaattt
     8161 atggattata aagatcttgc aattaaatgg ttcaataaag ctaacaacga cctgatcgca
     8221 ggaaattata ttctaactat gccagatcca cctgcagata ctatctgctt tcatagccag
     8281 cagtcagttg aaaaatatcc ctcagaatta tcaagttatg cggttgatgt cagatatccg
     8341 atggaaggaa attatgatgt cacaattgaa gaggcaaaaa gagccatcga cattgccgga
     8401 aaaatcaaga ttttcgtctt aaacaaatta gagttgagtt gatattcata atgcgaaaag
     8461 cacaaatatg gctcgatcag gtaaaaagtg agaataaacc tcaaagctcg gtaattattc
     8521 caataatgca taaacaagtg catcttacac gacgaggaac acggatgacg cagattgcgc
     8581 ggatcttcac ggatccgtgc gcatccgcgt catccacgca atccgtgttc tattgcacac
     8641 catctgcgtt catctgcgtt tatctgcggt taaataaatt gatttttcga caactagtac
     8701 ctcacaattt tggggaagga ttactttagt tacgagacct ctatgtattt aaacgtc
//
LOCUS       DATGBN010000366         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_107983, whole genome shotgun sequence.
ACCESSION   DATGBN010000366 DATGBN010000000
VERSION     DATGBN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107983"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VKL21_09045"
     CDS             <1..685
                     /locus_tag="VKL21_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45956.1"
                     /translation="SFTIPPDRRAALVEQAKALYSNDPAFKCGDIVGVPGTDNLDRIN
                     RINASDPVNPVHPVQTVASLSAAPDSRKILEFIGARLSAVPEESDVVHDLLAHLAERM
                     IEMNKEKNAEIKGFLRWFEGEIGAPVEELTNKIAIKEYYEAGFDALAGALAKNKKKLK
                     EGYDPTRREPKEKLQTEFNASVGKLAPLLKRIEATDKLIDQIVYKLYGLTPDEIKIVE
                     ESISGGKDL"
     gene            851..2035
                     /locus_tag="VKL21_09050"
     CDS             851..2035
                     /locus_tag="VKL21_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HMB45957.1"
                     /translation="MRKSFQFRIYPNKKQEVALERTLTTCRHLYNDSIAERKRNAELN
                     RLQKSFDVFPWGYPEWINYYDQANQLSLTKTAFQKEVYSQVVQNTLKRVERSFQNFFK
                     GAGYPRFQGRNRYNSFTYPGSGLKLNLNEGKLTLSKIGNIKIVLHREIEGKIKTCTIK
                     RDVDQWYVSFSCEIDTSTISTEIKTITGIDVGLISLMTFSNGEKIEPPKYLRASEKRL
                     AREQIHLSKKKKGSENRNKQRIVVAKVHRRIRFQRRDFNNKLSTNLVEKYDHVVFEKL
                     QIQNMLKNHCLAKSIVDASWSQLIDLTIYKAEYAGKIVELVNPNGTSQTCVCGYPVPK
                     DLSVRVHRCPSCGLVLDRDHVSAMIIESRSSTAGIAGIEARQSNPNREAMKREATLLV
                     GW"
     gene            2042..2392
                     /locus_tag="VKL21_09055"
     CDS             2042..2392
                     /locus_tag="VKL21_09055"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB45958.1"
                     /translation="MTSKRIKNPILAPPIRDILPELKEALQKRYGTKLKSIMLFGSYA
                     RGEQTASSDIDIAIILGEFSHACIEIERIGDIVSALSLKFDTLISLVPIKEQDWHTKK
                     TTLISNIKREGVAL"
     gene            2389..2538
                     /locus_tag="VKL21_09060"
     CDS             2389..2538
                     /locus_tag="VKL21_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45959.1"
                     /translation="MNEEMKALLKKAQDSIKGARILFDDELFGIDGNFIVLIDDARVY
                     RVQKM"
     gene            2546..2866
                     /locus_tag="VKL21_09065"
     CDS             2546..2866
                     /locus_tag="VKL21_09065"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMB45960.1"
                     /translation="MEISVNIKDDYLKWLQEISKETSTDKEELIKTSIIKYVQEYKLR
                     KAIRKYTDGSVSLGKASEIAGMPKRLLMYKLQEIGIPLNQSESDFDKGMDTLTKARKL
                     KKSS"
     gene            3075..3689
                     /locus_tag="VKL21_09070"
     CDS             3075..3689
                     /locus_tag="VKL21_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45961.1"
                     /translation="MSDKDSKAPIYIAIVVAVILIAAGGYYVLGGKITPVKTNEVKQT
                     PPVTAAKETSIAPKTTSETTSASAQTPAVTTAPAKVETIKCELCHKDAQDLTPHLNGG
                     KLCVTCHGSQVHNIHIGQGTVGLECNTCHGSPPKIPTVDKSADGPGHFSVCENCHAAP
                     PDSLKPSLGNLVTIHLSRAKYCTNCHGTDIGVIHSASLANATKK"
     gene            3742..>4247
                     /locus_tag="VKL21_09075"
     CDS             3742..>4247
                     /locus_tag="VKL21_09075"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HMB45962.1"
                     /translation="MTFFEQFAKVCNTIEHISGSLEITTNVANFLKEVSDEELPIVTR
                     FIMGHVFPVWSNKELGIGPNLLYCAISKTSSLPVKRIEGLIKETGDVGLALEKAIISG
                     KTHLKFFSEGEQLSIKEVYSRFEKISELNGKGSREAKIKNLQYLFSLARPTEIVYLSR
                     LSIEELRIG"
BASE COUNT         1439 a          825 c          931 g         1052 t
ORIGIN      
        1 ctccttcacc atccctcccg accgccgcgc cgcccttgtg gaacaggcga aggcgctgta
       61 ctccaatgac cccgccttta aatgcggaga catcgtggga gtgccgggta ctgataattt
      121 agacaggatt aacaggataa acgcttcaga tcctgtaaat cctgttcatc ctgtccagac
      181 ggtcgcgtct cttagcgcgg cgccggacag tcgaaaaata cttgaattca ttggcgcgcg
      241 cctctcggct gtgccagagg agagcgatgt ggtgcatgac ctgctcgcgc atcttgcaga
      301 gaggatgatc gaaatgaata aggagaagaa tgcagagata aaaggattcc tgcgctggtt
      361 tgagggggag attggcgcgc ctgttgagga actaacgaac aagatcgcaa taaaagagta
      421 ttatgaggct ggatttgatg cgcttgcggg tgcgctggcg aagaataaga aaaagctaaa
      481 agagggatat gaccccacaa ggcgcgaacc gaaagaaaag ttacagactg agtttaacgc
      541 ttcggtgggg aagctggcac cgcttttgaa gaggatagag gcgactgata aactcataga
      601 tcaaattgtt tataagctat acggattaac tccagacgag ataaaaatcg tggaagagag
      661 tatttcagga ggaaaagatt tataacgtgg ggcagagggg tagggaataa taccggactc
      721 gtccggtgga tgaatcgtac cccctttcat tgtaatacct atcccgaaaa aagtattgtt
      781 cagatagcat tttagggata ggcataacga atatatagta tcaaatccat atatagtttg
      841 ttcagacagc atgagaaaat catttcaatt tcggatatac ccaaacaaga agcaagaagt
      901 agctcttgaa aggactctca caacatgcag gcatctttac aatgattcta ttgcagaacg
      961 gaaacgaaat gctgaactta accgactcca aaaatcattc gatgtatttc cgtggggata
     1021 tccagaatgg attaactact acgaccaagc aaaccaactc tcattaacaa agacagcatt
     1081 ccagaaagaa gtatattctc aggtagtaca gaataccctg aagcgagtcg aacgaagttt
     1141 ccagaacttc ttcaagggcg caggataccc acggtttcag ggtaggaata ggtacaattc
     1201 attcacatat cctggtagcg gtctgaaact caatcttaat gaagggaaac tcaccctgtc
     1261 gaaaatcgga aacattaaaa tcgtattgca tcgagaaatc gaagggaaaa tcaagacctg
     1321 tactatcaag agagatgttg accagtggta cgtttcattc tcatgcgaaa ttgatacatc
     1381 gaccatttcg actgaaatca agaccataac aggaatagat gtcggtctga tttccctgat
     1441 gactttcagt aacggtgaaa agattgaacc accaaaatac ctgcgagcat ctgagaaaag
     1501 acttgcacga gaacagatac acctgtcaaa gaaaaagaaa gggtcggaga ataggaacaa
     1561 gcaacgtatt gttgttgcaa aggttcatcg aagaatcaga tttcagagaa gagatttcaa
     1621 taataaatta agtactaatc tggttgagaa atacgaccat gtagtatttg agaaattgca
     1681 aatacaaaac atgcttaaaa atcattgtct tgccaaatca atagtagatg cctcatggtc
     1741 acaattaatc gatttaacta tctacaaagc agaatacgct ggtaaaatag tggaactggt
     1801 taatccaaat ggaacatcgc agacctgtgt atgtggctat cctgtaccga aagacctatc
     1861 agtgagagta catcgatgcc cttcctgtgg attggtactg gatagagacc acgtatctgc
     1921 gatgataata gaaagcagaa gtagtacggc aggtattgcc ggaattgaag cccgtcagag
     1981 caatcccaat agggaagcaa tgaagcggga agccacccta cttgtagggt ggtagttcac
     2041 aatgacgtcg aaacgtatca aaaatcccat tttagcgccc cctattagag atatccttcc
     2101 ggaactaaag gaagcgctcc aaaaaaggta tggaacaaag ctcaaatcca taatgctatt
     2161 tggctcttat gcgagaggtg aacaaacagc cagctcggat atagatatag ccataattct
     2221 tggagaattt tcccatgcct gcatcgaaat cgagcgcatt ggagatattg tttcagcatt
     2281 atctctgaaa tttgacacgc tcatatcact tgtacccata aaagaacagg actggcacac
     2341 aaaaaagact actttgatct caaatataaa acgagagggg gtggctttat gaatgaagag
     2401 atgaaggcac ttttaaagaa agcgcaggat agcatcaaag gcgccaggat tttatttgat
     2461 gatgaattat ttggtattga tggaaatttc atagtcctta tagatgatgc aagggtttac
     2521 agggttcaaa agatgtgata ttgaaatgga aataagcgta aatattaaag atgattatct
     2581 gaaatggctt caagagatat caaaagagac atcaaccgat aaagaagaac ttataaaaac
     2641 atctattata aaatatgttc aggaatataa actcaggaaa gccataagaa aatacaccga
     2701 tggttctgtt tctctaggaa aagcatctga aatagcaggc atgccaaaaa gactgcttat
     2761 gtataaactt caggagatcg gaattccgct gaatcagtcg gaaagcgatt ttgataaagg
     2821 tatggacact ctgacgaagg ccagaaaact gaagaaatct tcctgatgtt gatgataatg
     2881 tcagtattcc aacgaaggta attcaatcaa agcagggtca tagcccttca tggataaaaa
     2941 agtgactgtt ccaatagaag tttataacaa cgtgaaatta taggaataac ctcaatacct
     3001 gttataattg attgcagata taaaccaaaa catttaaaac tacataatac cctaattagc
     3061 gtggtgatta tgtaatgagt gataaagatt cgaaagctcc tatttatatt gcgatcgtgg
     3121 tggccgtcat cctgatagcg gccggtggtt attatgtact gggtggcaaa attactccgg
     3181 tgaaaaccaa tgaagtaaaa cagacccccc ccgtaacagc agctaaagaa acatcaatag
     3241 cgccaaaaac aacctctgaa acaacctcag cctcagcgca aaccccggca gttacaacag
     3301 ctccggcaaa agttgaaacc atcaaatgtg aattatgtca taaagatgct caggatctaa
     3361 cgcctcattt aaacggagga aaactctgcg taacctgtca tggaagccag gtacataata
     3421 tccatatagg gcaaggcaca gttggcctgg aatgtaatac ctgtcatgga tccccgccaa
     3481 aaatacccac cgttgataaa tctgcagatg gacccggtca tttttcagtt tgtgaaaatt
     3541 gccatgctgc tccgccagat tcacttaaac caagtcttgg caatctcgta acaattcacc
     3601 tttcaagggc aaagtattgc acgaactgcc acggaactga catcggtgtc attcattccg
     3661 caagcctggc aaatgcgacc aaaaaataaa taaagggcca acaccctttt tatttttttg
     3721 tgtggatctt atttttttaa tatgactttt tttgaacaat ttgcgaaagt atgcaacacg
     3781 atagaacata tttcaggttc tcttgaaata acaacaaatg ttgcgaattt ccttaaagaa
     3841 gtaagtgatg aggaacttcc gatcgttacg cgttttatca tggggcatgt gttcccggta
     3901 tggagcaata aggaacttgg gatcggacct aatcttttgt attgcgcaat atccaaaaca
     3961 tcatccctcc cggtaaaacg aattgaagga cttattaaag aaacaggaga tgttgggctg
     4021 gctctggaaa aagcgattat ttccggaaaa actcatctaa aattcttctc cgaaggcgaa
     4081 cagctttcta ttaaagaagt atattcaagg tttgaaaaga tctcggaatt gaatggaaaa
     4141 ggctctcggg aagctaaaat aaaaaatctg caatatcttt ttagtctggc aagacccaca
     4201 gaaatcgttt atctttcacg gctgtcgatc gaagaattgc gtatcgg
//
LOCUS       DATGBN010000367         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_340922, whole genome shotgun sequence.
ACCESSION   DATGBN010000367 DATGBN010000000
VERSION     DATGBN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340922"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VKL21_09080"
     CDS             <1..746
                     /locus_tag="VKL21_09080"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HMB45963.1"
                     /translation="VNVTDNQRAFMRMSPLATCSILQGKGLETIMHITCRDRNRLALQ
                     SDLIGAASLGIRNVLVMSGDHPSKGDHEGAKPVYDLDSVQLLGMIRKLDQGFDLCGNE
                     LNGRTDFCAGAVTNTILNEVGLIKFRKKIKMGAVFFQTQAVFDVRGFAEFMEKTSEIK
                     SKKVKIIAGIIPLKSEGNAHFLNKVPGIRVPDDMIKKMKDAKDPKAEGMKIAAGIIRE
                     LRTMCDGVHIMPIGNHENTKSLLEMAGLI"
     gene            743..>1570
                     /locus_tag="VKL21_09085"
     CDS             743..>1570
                     /locus_tag="VKL21_09085"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMB45964.1"
                     /translation="MKTQLELAREGTETKQMQAIAKAENLDTGILRLRISNGSVVIMN
                     RKDVSIGIGLGLSTKVNVNLGTSSLKIDPDEEVKKARIAQMYGADTITDLSMGGNIYE
                     IRKMIMENTTLPLTTVPIYQSAAQKGLGNIDSDEIIKNLRQQAKEGMSSFVLHCINKK
                     TLDILKKKERIMGVVSKGGSITSAYMMLNNCENPFISNFEEILSILKKYDIVLSLGNT
                     MRSGCIHDKRDKAQIAEIKQNARLAKIANEAGVQAIIEGMGGHIRADRIAGYVRFHKK
                     "
BASE COUNT          529 a          292 c          379 g          370 t
ORIGIN      
        1 ctgtaaacgt aactgataac cagagggctt ttatgagaat gagtccactt gcaacatgca
       61 gtatcctgca gggaaaaggt ctggaaacaa ttatgcacat cacctgcagg gacaggaaca
      121 ggcttgctct ccagtctgac cttatcggag cagcctccct gggaatcagg aatgtgcttg
      181 taatgtcagg agaccaccct tcaaaaggag accatgaagg agcaaagcca gtttatgatc
      241 ttgattccgt gcagcttctc ggaatgatcc ggaaactgga tcagggattt gacctttgcg
      301 gaaatgaatt aaatggaagg acagattttt gtgcgggcgc cgtgactaac accatattga
      361 atgaggtagg gcttattaag ttcaggaaaa aaataaagat gggagctgtt ttctttcaga
      421 cgcaggccgt atttgatgta agaggttttg cagaatttat ggaaaaaacg agtgaaatca
      481 aatcaaaaaa agtaaagata attgccggga taataccttt gaaatcagaa ggaaacgcgc
      541 attttctcaa caaggttccc ggtataagag ttcctgatga tatgataaaa aagatgaaag
      601 atgcaaaaga tccgaaagca gaaggcatga aaattgccgc cggcataata agggaattac
      661 gaacaatgtg cgatggcgtc catattatgc ccatcggaaa tcacgaaaat accaaatcgt
      721 tgctggaaat ggcgggcctg atatgaaaac ccagcttgag cttgcaagag aggggactga
      781 gacaaaacag atgcaggcta ttgcaaaggc tgaaaatctc gacacaggga tcctgcgttt
      841 gcggatctca aacggaagcg ttgtaataat gaatcggaaa gatgtatcca ttgggatagg
      901 tttaggtctt tcaacaaaag tcaatgtcaa tttaggcacc tcctccttaa agatcgatcc
      961 tgatgaggag gttaaaaaag cccgaattgc ccagatgtat ggcgcagaca ccataactga
     1021 tctttccatg ggcggcaata tctatgaaat tcgtaagatg atcatggaaa atacaactct
     1081 ccccctgacc actgtcccga tttatcaatc ggctgcccaa aaaggtcttg ggaacattga
     1141 ttctgatgaa attatcaaga acttaagaca gcaggcgaaa gagggtatga gttcttttgt
     1201 gcttcattgc atcaacaaaa agacacttga tatcctgaag aagaaagaac gcataatggg
     1261 tgtagtctca aaaggcggat ccataacaag cgcatatatg atgcttaata attgtgaaaa
     1321 tcctttcatt tctaatttcg aggagatatt aagcatcctc aaaaagtatg atattgtgct
     1381 ctcacttggc aataccatga gaagcggatg tattcatgat aaacgggata aagcgcagat
     1441 agcagagata aaacagaatg ccagacttgc aaaaatagca aatgaggccg gggtccaggc
     1501 gatcatagag ggaatgggag gacatatccg cgccgacagg atagccggtt atgtaaggtt
     1561 ccataagaaa
//
LOCUS       DATGBN010000368        10153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_534739, whole genome shotgun sequence.
ACCESSION   DATGBN010000368 DATGBN010000000
VERSION     DATGBN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534739"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VKL21_09090"
     CDS             <1..605
                     /locus_tag="VKL21_09090"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HMB45965.1"
                     /translation="YDFYGFPQELYKIKYPCPGSPRDARYLIESVKKLNIKCSTNWGL
                     DHASWSILRHMYPAANIPVIELSLDYSFNEWNPKPVQYHYDIASELAILREKGILIIG
                     SSNIVHNLGLIDFRNIDATPYSWAVEFDEEVRSCLINRDHGKLIDHKKIKSSHLAVPT
                     LDHYLPMIYALALQEENEHLKFTYEGFQHGSISMRCFQIG"
     gene            complement(664..969)
                     /locus_tag="VKL21_09095"
     CDS             complement(664..969)
                     /locus_tag="VKL21_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45966.1"
                     /translation="MSIKKIKAIDGVEFLLSSWDDPKKGINAYQIMDEALRYLARIHE
                     AVKEVDKNEFSNDPMELCRLVLRKLNLPETAANPLIARSFEAHRKVSALYNHCNICD"
     gene            1056..1625
                     /locus_tag="VKL21_09100"
     CDS             1056..1625
                     /locus_tag="VKL21_09100"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HMB45967.1"
                     /translation="MTLSVYRWEDVHRYFPQEVIEQYRKKIIEEWKSGKFKDREIWER
                     YGMSENAFYDFIKRYSEEENLKDKPSKPKNPSHKLDQDDMQIRSDCGKEFYNEAVRNF
                     CNKNNIRLHFIPKGSPWLQAFIERGFRTIKEEFLNLVWIGNWNKFNDVLRDARYGYNH
                     RPNSAFDYKNPLEVMAIKIPNLPQQVCGH"
     gene            1934..2182
                     /locus_tag="VKL21_09105"
     CDS             1934..2182
                     /locus_tag="VKL21_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45968.1"
                     /translation="MPRTKTLHVIFDGNVLKPEEFVDLETDVRYLVTIERKEETSQQS
                     LWDTLNKFSGKVEGPEDWSKEHDHYLYGLPKREKGSIK"
     gene            2179..2592
                     /locus_tag="VKL21_09110"
     CDS             2179..2592
                     /locus_tag="VKL21_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB45969.1"
                     /translation="MKVNRFFLDTAYVLALINPKDSYHKHAKTMLPSMYSAHEVWMTE
                     AVLIEIANALASSNRSAAVAFINSCYITPNVRVVSVDSKLLKRAMVFYENRGDKGWGL
                     TDCISFIVMEDHDLKEAFTADDHFQQAGFRALLLE"
     gene            2585..2746
                     /locus_tag="VKL21_09115"
     CDS             2585..2746
                     /locus_tag="VKL21_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45970.1"
                     /translation="MNEDILFDMASLAGGADLNPAPSPPARRNFAAKLVRIVYWVLKN
                     NKEYQTQPN"
     gene            3086..3283
                     /locus_tag="VKL21_09120"
     CDS             3086..3283
                     /locus_tag="VKL21_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45971.1"
                     /translation="MRDRTHTEHIERWAKFVKEHPRSIWIREVGPLIDAQIIMANSFY
                     ERLAKVEGGIEKIKQLRKLNK"
     gene            3304..3870
                     /gene="cas4"
                     /locus_tag="VKL21_09125"
     CDS             3304..3870
                     /gene="cas4"
                     /locus_tag="VKL21_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HMB45972.1"
                     /translation="MAISNLTLKEEDKTQPAEIVTGAMRITGVKIAYYFICHTKLWLF
                     SHNITLEKENDNVNIGKLIHEDRYKRDSKEITIDQTISIDFIRKGDNIVLHEIKKTKS
                     MEAAHRWQMLYYLYYLKSKGVSVTGEINYPLVSRKEEIALLEKDELDMENVINDIRKI
                     AKGRMPQPKRIRICPKCAYFEFCFGDEV"
     gene            3867..4043
                     /locus_tag="VKL21_09130"
     CDS             3867..4043
                     /locus_tag="VKL21_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45973.1"
                     /translation="MKEDYYITQDGGLTRHENTVYFESSNCSMLKSALRGIEIKYQAT
                     FYCSFALCYKNMRM"
     gene            4160..4516
                     /locus_tag="VKL21_09135"
     CDS             4160..4516
                     /locus_tag="VKL21_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45974.1"
                     /translation="MKRKDNICQEGAVSYVHPESLLGYQVRKLRPACQFIPVPIACRA
                     GIHHKKRQGKHPLPARLSEAEHFFIYRLQLETDVAKIKTILPKKYLSEIYTLPEYHCF
                     YSDNTGIVKPLAPIRI"
     gene            4554..4775
                     /locus_tag="VKL21_09140"
     CDS             4554..4775
                     /locus_tag="VKL21_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HMB45975.1"
                     /translation="MSHKTLTISEEAYEALAGLKKEGESFTQLIKRITAPMKKRKLSE
                     FAGMIKDEEFKRAALEIRHHPSRLNRMKL"
     gene            4772..5176
                     /locus_tag="VKL21_09145"
     CDS             4772..5176
                     /locus_tag="VKL21_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMB45976.1"
                     /translation="MTVFDTNFLVDLLNDEPGISYIADSFEHPKTTTINAFELFYGAW
                     QSSKPRENIREINSLLKSLSILEVDLAAAQKAAEIHAGLKNSGSTLELEDVLIAGIVM
                     TNNEELVTRNINHFKRIPGLKYRSWQPGDYGR"
     gene            5166..5396
                     /locus_tag="VKL21_09150"
     CDS             5166..5396
                     /locus_tag="VKL21_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45977.1"
                     /translation="MEDKKIKELIGYSEENRKEILEIRIKEIQKHLNDLKKYLAKLNN
                     YRDRWTDYDVAVLCWLLAHDPDELKFWKEIEL"
     gene            5622..5921
                     /locus_tag="VKL21_09155"
     CDS             5622..5921
                     /locus_tag="VKL21_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HMB45978.1"
                     /translation="MKDAIKITREGVIIDLEISAGAKETAIQGYNAWRKRIEVRISQR
                     AQKGKANGELISFLSNLFDVNSKNIEIISGLTSSKKSVLISQKKPDEILDILSKK"
     gene            5918..6343
                     /locus_tag="VKL21_09160"
     CDS             5918..6343
                     /locus_tag="VKL21_09160"
                     /inference="COORDINATES: protein motif:HMM:NF003091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HMB45979.1"
                     /translation="MTQRDIENIERLEELEKTIVELKELALNGAILVVEGRKDAESLR
                     YLGIIGQIRLASQQPLLDLTESLSRSGKKIVLLTDWDNKGYLIAKKLEKHLLAYGIIP
                     DTRIRSKLRSLSKKSIKDIESLNNYINKLRYEVHGMMRF"
     gene            6465..7124
                     /locus_tag="VKL21_09165"
     CDS             6465..7124
                     /locus_tag="VKL21_09165"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HMB45980.1"
                     /translation="MKQKFYVMLGDVIASRRITDREIFQRKLEEACSNVNLNFVEDIY
                     ADFNILKGIDEIGGVLLNITNVYKIINTLQEQFFPHSMRFVLVFDYIDTAVESQEVSR
                     MDGPAFHRASDILKDLKGSRLMFKMSIGDEIMEKMMTGEINLIFLLKKSWSLRQRRII
                     YEYRKTGNQNIVANALGISQQAVSKALNRSMWKEISDIEEDLNYVLQYSPTLPKKIKE
                     W"
     gene            complement(7131..8405)
                     /locus_tag="VKL21_09170"
     CDS             complement(7131..8405)
                     /locus_tag="VKL21_09170"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB45981.1"
                     /translation="MNKNETIKATLKATKAKRKTQMCRVYETKIDLSHLNHATQEHLY
                     RLFLEAKWFYNYILSQHKIFGMDDKVHEVPVKVKDIFEIRDLRCLSSQMRQGLISRTQ
                     DNINSLSERKKKGYKIGKLKYKSEVQSIPLKQFGNTFKILDKRYITIQGLKQKLKVIG
                     LKQIPEGADIANGTLIHKNGNYYLSITTYQEKTKQISVVEKSVGIDFGLRRQLTLSRT
                     GIAIQYAIPVSPKLRKLARKLSKQELHSRNWNKTRIKLNKEYDKATNIKQDIKNKVVS
                     FLKDKYGTVCFQDENVKAWQRIWGRKILTTSIGGIISTLKKKVHTPIEVPKMYPSTKT
                     CSKCGNIQDIALDERVYVCKNKKCGNIMDRDYNSSINLENEGLKRLGTVRTELTPVEI
                     ESSTLVFLRYFNSIPYVKASSVNESGSFTALA"
     gene            8627..8842
                     /locus_tag="VKL21_09175"
     CDS             8627..8842
                     /locus_tag="VKL21_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HMB45982.1"
                     /translation="MATKTITIREDIYQMLLSIKGANESFSNFFERLVKSRKNIDKLK
                     DLRGCVEFRNKEALLKELSEKRMEIRY"
     gene            8839..9216
                     /locus_tag="VKL21_09180"
     CDS             8839..9216
                     /locus_tag="VKL21_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMB45983.1"
                     /translation="MILADTDVLIEIFDMNSKKGETSLNKIEEAGEDIALTSLNLHEI
                     LYGLYKYTDNPKIEKILTLDVVDFTKDDSILSAKLEVKAEKNGNKTPRFDTMIAAVAI
                     NRGFKLFTFNKKHFKEFKDLILF"
     gene            complement(9213..9428)
                     /locus_tag="VKL21_09185"
     CDS             complement(9213..9428)
                     /locus_tag="VKL21_09185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB45984.1"
                     /translation="MILISKRAEQIHIKIVEKAKKHLKVGDIVLDCGCATGTVAIEIA
                     DNVKKVHGIDISSKMKGEKEEQKTSNR"
     gene            complement(9403..9597)
                     /locus_tag="VKL21_09190"
     CDS             complement(9403..9597)
                     /locus_tag="VKL21_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45985.1"
                     /translation="MTKHITDISQRKAAIVAGLAFLLAIITTILKWIWLLIKGGNDQP
                     MKKTEKFWDMISNDFDKQEG"
     gene            complement(9617..10066)
                     /locus_tag="VKL21_09195"
     CDS             complement(9617..10066)
                     /locus_tag="VKL21_09195"
                     /inference="COORDINATES: protein motif:HMM:NF039408.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HMB45986.1"
                     /translation="MTNNITNKKTTEMEDMKVKLSTLWIFVMFNYLYCDIMGLMDPVL
                     LKQFMTGNVGGIQITQEFFLGAAILMEIPIAMVLLSRVLKYRANRWTNIVTGSTMTVV
                     QFSSLFFGSSPTIYYIFFSIIEISCTSLIVWYAWKWTNLEVSPNNKI"
BASE COUNT         3296 a         1822 c         2054 g         2981 t
ORIGIN      
        1 catatgattt ttatggattt ccacaggagc tatataaaat aaaatatcca tgtccaggct
       61 caccccgcga tgccaggtat ttaatagagt cagtgaaaaa gctaaatatc aaatgcagca
      121 ccaactgggg cttagatcat gcttcctggt cgattttaag acatatgtat cctgcagcga
      181 atattccagt tatcgaatta agtcttgatt attcttttaa tgaatggaat ccaaaaccag
      241 tacaatatca ttatgatata gcctcagaac ttgcaattct aagagagaaa ggaattctta
      301 taataggcag cagcaatatt gttcataatc ttggactcat tgattttcga aatattgatg
      361 caactccata tagttgggct gttgaattcg atgaagaagt tagatcatgt ttgataaata
      421 gggatcatgg aaagctgatt gatcataaaa aaataaaaag ttcccatctt gcagtgccga
      481 cacttgatca ttatttgcca atgatatatg cactggcatt gcaggaggaa aatgaacatt
      541 tgaagttcac atatgaagga tttcaacacg gatcgatttc tatgagatgt tttcagatcg
      601 gatgaactta cttgttcagt cttgccagac ttttttctat tttctgtaag atatatccct
      661 gattcaatca caaatattac aatgattata gagggcggaa acttttcgat gagcttcgaa
      721 agaccttgca attagtggat ttgctgccgt ctcaggcagg ttcagctttc gtagaacaag
      781 tctgcataat tccattgggt catttgagaa ctcattcttg tcaacctctt taactgcttc
      841 atggatgcgc gcaagatatc tcaatgcttc atccatgatc tggtatgcat taattccttt
      901 tttcggatca tcccatgaag aaagaagaaa ttcgacccca tcaattgctt ttatcttttt
      961 gattgacatg acagatgcta gggttagtgt ccactgacct catggggtaa tgttttaata
     1021 agtggagatt aattgaatat taagaataat taattatgac cttgagtgta tatcgttggg
     1081 aagatgttca tcgctatttc ccgcaggaag ttattgaaca gtatagaaaa aagataatag
     1141 aggaatggaa atctggcaag tttaaagata gagaaatttg ggaacgttat ggaatgagcg
     1201 aaaatgcatt ttatgatttc atcaagcggt actcagagga agaaaattta aaggataaac
     1261 caagcaaacc aaagaatcca tctcacaaat tagatcaaga tgatatgcag ataaggtcgg
     1321 attgtggaaa agaattttat aacgaggccg tgaggaattt ttgtaataaa aacaatatac
     1381 gtctccactt tatcccaaaa ggaagcccat ggctgcaagc tttcatcgaa agaggttttc
     1441 gaactattaa ggaggaattc ctaaatcttg tatggattgg taattggaat aaattcaatg
     1501 atgtgttgcg agatgccaga tacggatata atcatagacc caactctgca ttcgattaca
     1561 agaatccttt agaagttatg gctataaaaa tacctaattt accccagcaa gtgtgtggac
     1621 actaacatta gtattggtca ataccttaaa ttgagaaaag agaatctaat cgaacaagat
     1681 gaagatataa aattcgccag cgtggactac gataaggact taggtctttt gaaggatgaa
     1741 gggtcaactc tttggggaat atttaaatca taatccgcac tccggcacgc gctcccccga
     1801 cctcgcccat tacgccctca ccccgcgcgc cgcggaactt tgcggactct gcggtggata
     1861 cttatcatta cggtgcctgc agaggatagt atataaatgt gtgaaagacc ataaaaggta
     1921 ctagaggtgc aagatgccac gaacaaagac actgcatgta atttttgacg gtaatgtgct
     1981 caaacctgaa gagtttgtag acttagaaac agatgttcgt tatctcgtaa ctatcgagcg
     2041 taaagaagaa acaagtcagc aaagtctatg ggatacactc aacaaattct caggaaaagt
     2101 cgaggggcca gaagactggt caaaagaaca tgaccattac ttatatggac ttcccaaacg
     2161 agaaaaggga agcattaaat gaaagtgaac agattttttc tcgatacagc ttatgtattg
     2221 gcattgatca atccgaagga ttcctatcac aaacatgcta agacgatgct tccttcaatg
     2281 tattcggcac atgaagtatg gatgactgaa gcagtactga tcgagattgc caatgcgttg
     2341 gcaagttcaa atcgttccgc tgccgttgct ttcattaata gttgttatat tacaccaaat
     2401 gtccgggttg tttcagtgga cagcaagcta ttgaagcgcg cgatggtttt ttatgaaaac
     2461 cgtggagata agggttgggg gttgactgat tgcatctctt ttatcgtaat ggaagaccat
     2521 gatttgaaag aggcctttac cgcagacgac cactttcagc aggcaggctt tcgggcactg
     2581 ttacttgaat gaagacattc tatttgacat ggcgtccctt gccggtggag cagacctcaa
     2641 ccctgcgccc tcacccccag cgcgccggaa ctttgctgca aaattggtga ggattgttta
     2701 ctgggtcttg aagaataaca aggagtatca gacacaaccg aattaaattt agtctattta
     2761 aagggaaacc agacaaacaa attgtggata ctatttaatt atatcttgca agtattgaca
     2821 attgtgatat tatgcaacca gtgatagctt attaaacccc gccagcggcg gcgcatccgc
     2881 tggggtctgg ggtcgaaacc ccagcgccgg aaatttattg aatagttata aaattcactg
     2941 caaactcagg tgggtatgtg gaaaggcata ttatctcgca aatttattga ataacctgaa
     3001 tgagcaggga agtgaaagag cggacaattc ttgatgaaaa tgagatcaca aagataaaag
     3061 cccagatccg cagggtgcgc ctatcatgag agacaggacc cataccgagc atatcgagcg
     3121 atgggctaaa tttgtaaaag aacatcctcg ctcaatctgg atcagggagg ttgggcccct
     3181 gatagacgcg cagataatta tggcaaattc attttatgag cgccttgcta aagttgaagg
     3241 tggaattgag aaaataaaac agctaaggaa gttaaacaaa taaattaaat ataccggagc
     3301 cggatggcca tatcaaattt aacattgaaa gaagaagata aaacacaacc cgcagaaatc
     3361 gtcacaggag ccatgcgcat tacgggtgtt aagatcgcat attactttat ctgccataca
     3421 aaactctggc tattttcgca taacatcaca cttgagaaag agaatgataa cgtaaatatc
     3481 ggaaaactga tccatgaaga ccgctataag cgcgactcaa aagagataac gatcgaccag
     3541 acgataagca tcgattttat ccggaagggg gataacattg ttctccatga aataaaaaag
     3601 acaaaaagca tggaagcggc acatcgctgg cagatgctgt attacctgta ttacctgaaa
     3661 tcaaaaggcg tcagcgtcac aggcgaaata aattatcccc tcgttagcag gaaagaggag
     3721 atcgcgcttc ttgaaaaaga tgaattggat atggaaaatg tgataaatga tatcaggaag
     3781 atagcgaagg gaaggatgcc gcagccaaag aggataagga tctgcccgaa atgcgcttat
     3841 ttcgaattct gtttcggaga tgaagtatga aagaagatta ttatattaca caggacggcg
     3901 gccttacaag gcatgaaaat acggtatatt tcgagtcaag taattgttca atgttaaaat
     3961 cagctcttag agggattgaa ataaaatatc aggctacttt ttattgcagt tttgcactat
     4021 gttacaaaaa catgcgtatg tagtggaacg attgtggtac tctgatagaa caacgggact
     4081 gtgggtagaa agccaattca taacgaagta ggagaatgca tataaaaagc aataactgtg
     4141 tcttcatcgc aggaaaaaca tgaagcggaa agacaacatt tgtcaagagg gtgctgtatc
     4201 ctatgtacac ccggagagtc ttctgggata tcaagtgcga aaactccgac ctgcttgtca
     4261 attcatccct gtgccgatcg cctgcagagc tggcatccac cataaaaaaa ggcaaggtaa
     4321 gcatcctcta ccagcccgct tatcagaagc cgaacatttt ttcatttacc gcctgcagct
     4381 tgagactgac gtagccaaga tcaagacgat cctgccaaag aagtatctat ctgaaatata
     4441 cacgcttccg gaataccatt gtttttattc tgataatacc gggattgtca aacctctcgc
     4501 gccgattcgg atatgagaaa attataaata cacgtaagat tacacttaat tacatgtcac
     4561 ataaaacact tacaatttcg gaagaagctt atgaagcgct ggcagggctt aaaaaagaag
     4621 gtgaaagctt cactcaactt ataaagcgca tcactgcacc aatgaagaag agaaagctca
     4681 gtgaatttgc gggtatgata aaagatgaag aatttaaaag ggcagcgctt gaaataagac
     4741 accatccaag cagattgaat agaatgaagc tatgacagtg tttgatacta atttcttagt
     4801 tgatctcctt aacgatgagc caggaatatc gtatatagct gattcattcg agcatccaaa
     4861 aacgactact ataaacgcct ttgaactctt ctacggagct tggcaatcat caaaaccaag
     4921 agaaaatatc agagaaatca actccttatt gaagtcttta agcatacttg aggttgattt
     4981 ggctgctgca cagaaagcag cagaaattca tgcagggttg aaaaactccg gatctactct
     5041 ggagctcgag gatgttctaa tagcagggat tgtaatgaca aataatgaag agcttgtgac
     5101 caggaatatt aatcatttca aacgcattcc aggtctgaag tacagatcat ggcagccagg
     5161 tgattatgga agataagaag attaaagaat tgatcggtta ttcggaagaa aatagaaaag
     5221 agatattaga gatacgaata aaagaaatac aaaaacatct aaatgatttg aaaaaatatc
     5281 ttgcaaaatt gaataattat agagatagat ggacagatta tgatgtggcc gttctttgtt
     5341 ggttgttggc tcacgatccg gatgagttaa aattctggaa agaaattgaa ctttaaaaac
     5401 ctcttcatgg caaaagaagg ttaatacatc ttccttttta tgtttttgaa gctataaaga
     5461 tgtattaagt aagaaggtgg tgttttttca aaaaagtccg atcgtgtttt tccggaaaaa
     5521 gactggatag agtgtggaat attctgcaat ggaagattat attgttgtaa aaagataaag
     5581 agagcaatat agttaaaagc agcccttgga gggattgaaa tatgaaagac gccatcaaaa
     5641 ttacccggga aggagtaatt attgaccttg aaatatcggc aggggcaaaa gaaactgcca
     5701 ttcagggata caatgcatgg cgcaaacgaa ttgaggtaag gatctcacag agagcgcaaa
     5761 aagggaaagc taacggggaa cttatttcgt ttttatccaa cttattcgat gtcaattcaa
     5821 aaaacataga gataatttca ggtttgacaa gcagcaagaa atcggttttg atatcacaga
     5881 aaaaacctga tgagatactg gatatcctga gcaagaaatg acgcagaggg atatcgaaaa
     5941 tatcgaacgc ctggaagaac ttgaaaaaac tatcgtggaa cttaaggaac ttgccctgaa
     6001 tggcgcaatt cttgtggtgg aaggcaggaa ggatgcggaa tccctgcgtt atcttggaat
     6061 cattggccag atcaggcttg cttcacagca gccattactt gatcttacgg aatctctctc
     6121 aaggagtgga aaaaagatcg ttctattgac ggattgggat aataaaggat acctgattgc
     6181 aaaaaaatta gaaaagcatc ttctagctta tgggataatt cctgatacgc gcatccggtc
     6241 aaaactaagg tcgctttcaa agaaaagcat caaagacatt gaaagcctca acaattatat
     6301 aaataaatta agatatgagg tacatgggat gatgagattt tagaacaatc ctataaaatt
     6361 cgcaatatca gtttgttaga aaaaatacaa tcaaaataag ttgtttttta ataaacaaac
     6421 aaaaaggatt gttttataag ccataaatac catatataac ttaaatgaaa caaaagttct
     6481 atgtgatgct gggcgatgtt atagcttccc gcaggataac ggatagggag atattccaga
     6541 gaaaactgga agaagcatgc tcgaatgtga atttgaactt cgttgaggat atttatgcag
     6601 atttcaatat cctgaaaggg atagacgaga ttggaggtgt actcctgaat ataactaatg
     6661 tttataagat aataaacaca ctccaagaac agttttttcc acattctatg agattcgtgc
     6721 tggtctttga ttacattgat actgcagtag aaagccagga agtatcaagg atggacggac
     6781 ctgcatttca cagggcttcg gatattctga aagacctcaa aggttcaaga ttgatgttca
     6841 aaatgtccat tggcgatgag ataatggaaa aaatgatgac aggagaaata aatttaatct
     6901 ttctactgaa aaaaagctgg tccctccgac agcgcaggat aatctatgaa tatagaaaga
     6961 cagggaacca gaatatagtg gcaaatgctc tgggtatttc gcagcaagct gtatcaaagg
     7021 cgctaaatcg ctctatgtgg aaggagatat cggacatcga agaggattta aactatgtac
     7081 tccaatattc accgacacta ccgaaaaaga taaaggagtg gtgagctacc tcacgctaaa
     7141 gctgtgaagc ttcctgattc attgaccgag cttgctttga cgtatgggat actgttgaag
     7201 tatctcagga acaccagagt agaggactca atctccacag gcgttaattc ggtacgcacc
     7261 gtacctaatc gttttaatcc ttcattttca agatttatgc ttgaattata gtccctgtcc
     7321 atgatattac cacatttctt attcttacaa acataaaccc gttcatccag agctatatcc
     7381 tgaatgtttc cgcattttga gcatgttttg gttgatggat acatcttagg cacttcaatc
     7441 ggagtatgca cctttttctt gagtgtgctt attatcccgc cgattgaggt tgttaagatt
     7501 ttcctgcccc atatcctctg ccatgctttt acattttcat cctgaaagca tactgttccg
     7561 tatttatctt tcaagaagct aacaacctta tttttaatat cttgcttgat gttagtcgct
     7621 ttatcatatt ccttattgag tttgatcctt gtcttgttcc agttcctgct atggagttcc
     7681 tgcttgctga gttttcgtgc aagtttcctg agcttcggac tgactggaat agcatattgg
     7741 atcgcaatcc cggttcttga aagcgtcagt tgtcttctaa gaccgaaatc tattccaacc
     7801 gatttttcga ccacagatat ttgttttgtc ttttcctgat aggtcgttat cgataagtag
     7861 tagttaccat ttttgtggat cagcgttcca tttgcaatat ctgccccttc tggtatttgc
     7921 tttaatccaa ttacctttag tttctgtttt agaccctgta tcgttatata ccgtttatcc
     7981 agaattttaa aagtattgcc aaattgcttc aaagggatgg actggacttc gcttttgtat
     8041 tttaattttc ctatcttata gcccttcttt ttgcgttctg ataaactgtt aatattatcc
     8101 tgtgtcctgc tgatcagtcc ttgcctcatc tgagaagaaa ggcaacgcag gtcacgaatt
     8161 tcaaatatat ctttgacctt tacaggaact tcatgaactt tatcatccat tccaaaaatt
     8221 ttatgttgag atagaatata attatagaac cattttgctt caaggaataa tcggtacagg
     8281 tgttcctgtg ttgcatggtt aagatgagat aagtctattt tggtttcata aactctgcac
     8341 atttgagtct tacgctttgc ttttgtagct ttcagagtgg ctttgatagt ttcattctta
     8401 ttcatctgca accttaacaa actttatatg gttttattcc tatttattct ttgcctatct
     8461 caaaaatgca gccttaacat ctgtttttcg agataggtat tacaattcca aggggtacga
     8521 ttcatcatcc tgaaggcgtg aagctttctc taccccttga ccccacaccg taaggcggcg
     8581 ctttcttgag ttaaaattaa atatatgttt aaacatatgt ttggatatgg caacaaaaac
     8641 cattacgata cgagaagata tttatcaaat gctcctctcc attaagggtg caaacgagag
     8701 ctttagcaat ttttttgaac gacttgtaaa atcccgaaaa aatattgata aacttaagga
     8761 tttgagaggt tgtgttgaat tcaggaataa agaggctctt ttaaaagagc tgtctgaaaa
     8821 aagaatggag ataagatatt gatccttgct gatactgatg ttcttattga aatcttcgat
     8881 atgaactcga aaaaaggtga aacatcttta aataaaattg aggaggctgg agaagatatt
     8941 gctcttactt cattgaattt gcatgaaatt ctctatggtc tttacaaata taccgacaat
     9001 ccaaaaatcg agaaaatact gacgcttgat gtcgttgatt tcacaaagga tgattcaata
     9061 ctttcagcca aacttgaagt aaaagctgaa aaaaatggga ataaaactcc ccgatttgat
     9121 accatgatcg cagcagttgc aataaacagg gggtttaaat tgtttacttt taataaaaaa
     9181 cattttaaag aatttaaaga tctgatcttg ttttagcgat tgcttgtttt ctgttcctct
     9241 ttttctccct tcattttgga cgatatatca ataccatgaa cctttttcac attgtcagca
     9301 atttcaatgg ctaccgttcc tgtcgcacat ccacaatcta aaacaatatc gccaactttg
     9361 agatgttttt ttgccttttc aacaatcttg atgtgaatct gttcagccct cttgcttatc
     9421 aaaatcattt gatatcatgt cccagaactt ttctgttttt ttcattggct ggtcgtttcc
     9481 gcctttgatc aaaagccata tccatttgag tatagtggtt attatggcaa gcagaaacgc
     9541 taaccctgcg actattgcag ctttacgttg tgatatgtcg gtaatatgtt ttgtcatttc
     9601 aattactctt aatgtattag attttattat taggactaac ttcaaggtta gtccacttcc
     9661 atgcatacca gacaattagt gaagtacacg aaatttctat aatgctaaaa aagatataat
     9721 atattgttgg tgacgatcca aaaaataatg acgagaattg aacaacagtc attgttgaac
     9781 ctgtgacgat gttggtccag cgatttgctc tatatttcaa aactcgagaa aggagaacca
     9841 tagcaatcgg aatctccatt aatattgcag ctcccaagaa gaattcttga gtgatctgaa
     9901 taccacctac gtttccggtc ataaattgct ttaataacac aggatccatt aatcccataa
     9961 tatcgcaata aagataatta aacattacaa atatccacaa ggttgaaagt ttcactttca
    10021 tatcttccat ttctgtggtc tttttgtttg taatattgtt tgtcatttca attcctccta
    10081 atttgttagg ttttgttctt atgttctaca gttatgacta cacttgcctt tttgtgccct
    10141 ttttcaatat atc
//
LOCUS       DATGBN010000369         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_68590, whole genome shotgun sequence.
ACCESSION   DATGBN010000369 DATGBN010000000
VERSION     DATGBN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68590"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VKL21_09200"
     CDS             <1..398
                     /locus_tag="VKL21_09200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HMB45987.1"
                     /translation="LALVIAAAMAMLEFKILDPVPLITNLKIQNILLISIVMFIFVAA
                     IEELIFRSLLQTRLQSVFGINKGLLLGAALFGIMHSGYGNVEEVLFATFFGAVLGFIF
                     QKTRSFPFILIIHGTANVFLFGILPIVSK"
     gene            395..556
                     /locus_tag="VKL21_09205"
     CDS             395..556
                     /locus_tag="VKL21_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45988.1"
                     /translation="MIFTIRITAIVIRRAMEQVLRVLEAQLLQYFESEIRSAMERETI
                     SDRVAITPP"
     gene            complement(634..1425)
                     /locus_tag="VKL21_09210"
     CDS             complement(634..1425)
                     /locus_tag="VKL21_09210"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HMB45989.1"
                     /translation="MFRTAAVQKNLLIMIGILLLSYVSVLLLSQEDISDYTVADTEYW
                     RESGNRLLVKATYDYNSKELDAFPKTIGEWNGSDYRYPEGVYKTLKADVLLSRTYTKN
                     DGSLVWMDIINSKVGESFHQQKICVEGAGWKINNESIAEFKIGESTNLFAKLRANRLD
                     YSKKDQKQIMVYWFMFKKLGSKDSVAMIRLTSPVITNETDTYNLINGFVEDNLFRTMY
                     NQVGQENITVAEKIMNTYGNLGKLGVAMVILIPIGIIFAGIRRKN"
     gene            complement(1389..2204)
                     /gene="xrt"
                     /locus_tag="VKL21_09215"
     CDS             complement(1389..2204)
                     /gene="xrt"
                     /locus_tag="VKL21_09215"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HMB45990.1"
                     /translation="MNYLLIVFIGIIGVFYYNTIGWMIESWMYNEYYSHGFIVPIVSG
                     YMIWNMRHVLATIEKKPSQGGLGIFMAGILLQLISGVWTIRFLSGLSLILTLAGVIIY
                     VFGWDFMKKIKFPFLFLLFMIPFPFVDIIASPAQTFSAFASTNIANIIGIPARLDGLV
                     IAIPGGAFEVGITCSGINSIISLLTIGALFALILEGSRSMKFTILASAIPLAMAGNIL
                     RITSVLAVATVYGQTVALNYFHDFSSLLFFSISLMGLFLVGRCFGRLQFKKIF"
     gene            complement(2312..>3168)
                     /locus_tag="VKL21_09220"
     CDS             complement(2312..>3168)
                     /locus_tag="VKL21_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMB45991.1"
                     /translation="LQTKQHVDNVVVVDDGSSDRTGEMAKLAGAKVIRHTRKMGKGAA
                     LKSGFEYVGRNGAKVIVTMDFDGEHNPDYIPILINPILKGEADVVNGRWHKEGNGKKS
                     HFYQFIWKYSLDNVSNSNTRLSTTDTQNGIQAFARHTLTAFKFKSNGKTTGNDMLNDA
                     ANAGLRVKEVEIGVKQSVDCTTQNHGNRFLHPLVRMLEDIELCRPLYYLTIPGMLCTI
                     IGMGLGLDILNDFFMGENLKFWPMLFMIVLTLLGVFMAFTGIILHSVSRLINENNKTR
                     NTNSRGIP"
BASE COUNT          933 a          679 c          538 g         1018 t
ORIGIN      
        1 ctttggctct tgtgattgcc gcagcaatgg cgatgcttga gtttaaaata cttgatccgg
       61 ttcctcttat aacgaattta aaaattcaga acattctcct gatatctatt gtgatgttca
      121 tatttgtggc cgccatagaa gagctaatct ttcggtcatt attgcagaca agacttcaat
      181 ctgtgtttgg tattaataaa ggacttttgc ttggcgctgc gctttttggg ataatgcact
      241 ccggatatgg caatgttgaa gaggtactat tcgccacatt tttcggggct gttctgggtt
      301 ttatattcca gaaaacaagg agttttccgt ttatcctgat cattcatgga actgcaaatg
      361 tatttctatt tggcatacta cctatagtat caaaatgatc tttacgatca ggataactgc
      421 aatcgttata agaagagcga tggaacaggt gttaagagta cttgaagccc agttgctcca
      481 gtattttgaa tctgagatca ggagtgctat ggaaagggag acaattagcg acagagtagc
      541 cattaccccg ccttgattga tagtctccac aggaaatgaa gtctgtacgt ttgatataac
      601 agatattata ctcataaata tatgatcatc tattcaattt tttcgtctaa tacccgcgaa
      661 aatgatgcca atgggtataa gtattaccat tgctactccc aatttaccta aattcccata
      721 agtgttcatg atcttctctg ctactgtaat gttctcctgg cctacctgat tgtacatagt
      781 tctgaataaa ttatcttcca caaaaccatt gatcaaatta tatgtatcgg tctcatttgt
      841 gataacagga gaagttaatc ggatcatggc aaccgaatct tttgatccca atttcttgaa
      901 catgaaccag taaaccatta tttgtttttg atcttttttg ctgtaatcaa gtctattagc
      961 gcgtaatttt gcaaagagat ttgtggactc tcctattttg aactcagcta tgctctcatt
     1021 atttattttc caacctgccc cttctacgca aattttttgc tgatggaaac tttctccgac
     1081 cttactattt ataatgtcca tccaaaccaa actcccatca ttttttgtgt atgtccggct
     1141 cagaagcaca tcggctttta aggttttata aactccttca ggatatctat aatcagaacc
     1201 gttccactct ccgatagttt ttggaaatgc atccaattcc ttactattgt agtcatatgt
     1261 tgctttcacc aataggcgat ttccgctctc tcgccagtat tcggtgtctg cgacagtata
     1321 atccgaaata tcctcctggg atagcaataa tactgaaaca tatgaaagga gcagtatgcc
     1381 tatcattatt aaaagatttt tttgaactgc agccgtccga aacacctccc caccagaaat
     1441 aaccccatca aggaaatgct gaaaaaaaga agacttgaga aatcatggaa ataatttaat
     1501 gcaacggtct gtccatatac agttgcaact gccaataccg aagtgatcct gagaatattc
     1561 cctgccatgg caagaggtat tgcagatgcc agtatagtaa acttcattga tctactacct
     1621 tccagaatta aggcaaacag ggctccgatc gttaacaacg atatgattga atttataccg
     1681 ctgcaggtaa tccctacctc aaatgcccct ccaggaatag ctatgaccaa cccatctaac
     1741 cgggccggta ttccaataat atttgctata tttgtagaag caaatgctga aaaagtttgg
     1801 gcgggagatg ctatgatgtc cacaaaagga aagggtatca taaatagcag gaaaaggaat
     1861 gggaatttga ttttcttcat gaaatcccat ccaaaaacat atattataac acctgcgaga
     1921 gtcaatatga gagataaacc tgacaggaat cttatggtcc ataccccact tattaactga
     1981 agaagaatac ctgccataaa aatccccaga cctccctgcg atggtttctt ttcaatggtc
     2041 gcaagaacat gtctcatatt ccatatcata tatcctgaca cgataggtac tatgaatcca
     2101 tgggaataat attcattata catccacgac tctatcatcc acccaattgt attataatag
     2161 aaaactccta ttatcccaat aaatacaatt aaaagataat tcatttcaaa acctttttta
     2221 tacctcgttt aatctcgttt attatataaa tatattggag gatattgtcc taaaaaaatt
     2281 tgcatttccc ctgatctttt ctaatatatc cttagggaat tcctctgctg tttgtatttc
     2341 ttgttttatt gttctcattg ataagccttg acacagaatg cagtattata cctgtgaatg
     2401 ccatgaaaac ccctaatagt gtcaaaacta tcataaaaag catcggccaa aatttcaaat
     2461 tctctcccat aaaaaaatca tttagaatat ccaatcccag acccatgcca ataatagtgc
     2521 acaacattcc agggattgtc aaataataca gcggtctgca cagctctata tcttcaagca
     2581 ttcttaccag gggatgaaga aaacggttcc catgattctg tgttgtgcag tcaacacttt
     2641 gtttcactcc tatctctacc tctttcaccc tcaatccggc attagcagca tcgttgagca
     2701 tatcgtttcc tgtagttttc ccattcgatt tgaatttgaa tgcagtcagc gtatgtctgg
     2761 caaatgcctg aattccgttt tgcgtgtcgg ttgtggataa tcttgtatta gaatttgaaa
     2821 cattatcaag actatatttc catatgaact ggtaaaaatg tgattttttt ccattacctt
     2881 ccttatgcca tctgccgttt actacgtctg cttctccttt gagtatggga tttataagaa
     2941 tagggatata atccgggttg tgctcaccgt caaaatccat agttacaatg actttcgctc
     3001 catttcgccc aacatattca aaaccagact taagcgccgc acctttcccc attttcctgg
     3061 tatggcggat cacttttgca ccggcaagtt ttgccatttc tcccgtccgg tcagagctgc
     3121 catcatctac gactactaca ttgtccacat gttgctttgt ctgaagta
//
LOCUS       DATGBN010000370         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_341005, whole genome shotgun sequence.
ACCESSION   DATGBN010000370 DATGBN010000000
VERSION     DATGBN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341005"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1489
                     /locus_tag="VKL21_09225"
     CDS             251..1489
                     /locus_tag="VKL21_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HMB45992.1"
                     /translation="MSAIKIDPNIKYLEGTQRVFDPETTLSKTTKLLPKIGVTRIANI
                     TDLDRVGIPVFSAIRPSAAAGAISIYSGKGATETNARISAIMESFERCLAEQPEVSVN
                     LSGIKLNTETVTDSYESLCESYPTLYPDALLLPQPISEFTSLEWVKGDDILNDVEVFV
                     PANAVFHPYDPQSGTKLMRSNTNGLASGNTIEEAVLHGLLEVIERDALSIAEYTRNPG
                     KEIALTEKDGLNYKLKRKMEEAGINVKLWLLDSDIDIPTVVTALDDLVLKDPALLVMG
                     AGSHLSPEIAVTRALTEAAQSRVVQIHGAREDTDREQVVRTFGYDHMRRMNSYWYEDL
                     EKVHMDELEDISNNTPAANIKTVLEKLDGIADGAIIANLSRGVDIPVIRAIIPMFELY
                     TLDRERKGDRIKKKKKRVVK"
     gene            1486..2130
                     /locus_tag="VKL21_09230"
     CDS             1486..2130
                     /locus_tag="VKL21_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HMB45993.1"
                     /translation="MKPVVFTGTSISHSDAQKILDADYRQPVRRHDIRRLLKTSPEII
                     GIIDGVFFDSAAVAHREIIEALNSGIIVVGGGSMGALRAYELESYGMIGIGKVFEMYR
                     NGIIEADDEVAVTFDEESNEALSIPLVNIRMTVESAMNAGILNSGQGTAIIEIARKLF
                     YPDRNYQNVIFESVKAGIITDIEKKKLSDFIQTNEVDIKRQDAILVLEKIRQLL"
     gene            complement(2151..2435)
                     /locus_tag="VKL21_09235"
     CDS             complement(2151..2435)
                     /locus_tag="VKL21_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HMB45994.1"
                     /translation="MSRYIIISNLTDEGAMTLKKNPGRIKEVNDELKKMGVNVIDQYA
                     VLGNFDFISIVEAQDDNMVMKADVEIASRGSIKTETYKIIPITEFINALK"
     gene            2640..>3160
                     /gene="psmB"
                     /locus_tag="VKL21_09240"
     CDS             2640..>3160
                     /gene="psmB"
                     /locus_tag="VKL21_09240"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HMB45995.1"
                     /translation="MDNMEYKGTTTIGLVCKEGVVLATEKRATMGHFIASKDAKKVYQ
                     IDDLVAMTTAGSVGDAQRLVKWMQVESKLYKMRREEPMTVKGIVSLLANILSGNRYYP
                     YYVQLLVGGVDKHGPGIYSLDAVGGIIEEKKAVSTGSGSPMAYGVLEDRYVENMPIDD
                     GVALAIRALHNAM"
BASE COUNT         1004 a          597 c          697 g          862 t
ORIGIN      
        1 ttctcaacaa ctactcttgt ttttccgaga gcttccatca catgtctgtc ttttgccatg
       61 atcttttata tatgtcttat aagtattaac cattaagata ctgacaaaat tataggagtt
      121 gaggcaaaat ttgcctataa tatatgattt gacttgagca gatttagatg aaccatttct
      181 ccagaataaa ttaaaaaatc tcctgcttaa ggcacaagat atataactct tatcaaataa
      241 gaaagaaaaa atgagcgcta taaaaataga tccgaatatc aaatatcttg aaggcacaca
      301 gcgcgtattt gaccctgaaa ccacgctttc aaaaaccaca aagcttcttc caaagatagg
      361 agttacccgt atagccaata tcactgacct tgaccgagta ggaatcccgg tcttctcagc
      421 tataaggcca agcgcggctg caggcgcaat atccatatac tctggaaaag gcgcgactga
      481 aacaaatgcc aggatatctg caataatgga gagttttgag cgttgcctcg cggagcagcc
      541 tgaggtaagc gtcaatctta gcggaatcaa attgaacaca gagacagtta ccgatagtta
      601 cgagtcgctc tgtgagagct atcccaccct gtaccctgat gcgctcctcc ttccgcagcc
      661 gatttctgag ttcacgagcc ttgaatgggt caagggggat gacattttaa acgatgtaga
      721 agttttcgtt cctgcaaatg cggtttttca tccttatgat ccccagagcg gtaccaagtt
      781 gatgagaagc aatacgaacg gccttgcttc gggaaatact atcgaggaag cggtacttca
      841 cggattactt gaagtaatag aaagagatgc gctcagcata gcggaatata cccgcaatcc
      901 cggaaaagaa attgcgctta ctgaaaaaga cggattgaat tataaactta aaagaaaaat
      961 ggaagaagcc ggcattaatg taaaactctg gcttcttgat tctgatattg atattcctac
     1021 ggtcgtaaca gcgcttgatg acctggtact caaagaccct gctcttcttg ttatgggtgc
     1081 gggttctcat ctttcccccg aaattgcggt cacaagggcg ctaacagaag cagcgcagag
     1141 ccgggtggtc cagattcacg gggcgcgcga ggatacggat agggagcagg tagtgcgaac
     1201 atttggctat gatcatatga gaagaatgaa cagctactgg tatgaggatc ttgaaaaagt
     1261 ccatatggat gaattggagg acatctcaaa caatacgccg gctgcaaaca ttaagacagt
     1321 tttggaaaag cttgacggca ttgccgacgg tgcaataatc gcaaatcttt caaggggtgt
     1381 agatatccct gtaataaggg cgataatccc catgtttgaa ctatatacgc ttgatcgcga
     1441 aagaaagggt gatcgcatca agaaaaagaa aaaaagagtt gtaaaatgaa acctgttgta
     1501 ttcacgggta caagtataag ccacagcgat gcccaaaaaa tactggatgc cgattaccgt
     1561 cagccggtga ggcgccatga tatcaggaga cttcttaaaa catcccctga aataatcggg
     1621 ataattgacg gtgttttctt tgacagcgca gcagtggctc accgcgaaat aattgaagct
     1681 ctaaacagtg gaataattgt tgtgggaggg gggagtatgg gggcgcttcg ggcttatgag
     1741 cttgaatctt atggaatgat aggcattgga aaagtatttg agatgtatag aaacgggatc
     1801 attgaagcag atgatgaagt tgctgtgaca tttgatgaag aatcaaatga agcgctttca
     1861 attccacttg taaatataag aatgacagtt gaatctgcaa tgaatgccgg gatcctgaat
     1921 tccggacagg gaactgccat tattgagatc gcgcgaaagc tattctatcc tgacaggaat
     1981 taccaaaacg taatatttga aagtgtaaag gctggaatta tcacggatat tgaaaagaaa
     2041 aaattatctg attttataca aacaaatgaa gttgatataa aaaggcagga tgccattctt
     2101 gttcttgaaa agatcaggca acttctctga atccaatatt caaacgcgag ttatttcagc
     2161 gcattgatga actcggtaat gggtattatt ttataagtct cggtctttat gctgccacgg
     2221 gaggcaattt caacatcagc cttcataacc atgttatcat cttgagcttc aacaattgat
     2281 atgaagtcaa aatttcccag aacagcatat tgatctatta catttactcc cattttcttc
     2341 aattcgtcat ttacttcttt tattctgcca gggttcttct ttaaagtcat tgcaccttca
     2401 tctgtaaggt ttgaaattat tatatatctt gacataatat tcctccattt cccatgttat
     2461 attcggttgt ctttctatat atatgttttt tcaaaaataa tcgtactcaa catcaaaaaa
     2521 taattatgta aatgaagtta gacttaccac atcggagcaa aaacctttaa atacacctaa
     2581 acagattata ttatgctaat tttctcattt tttgtgatga ggtttaagag gtatttaaaa
     2641 tggataatat ggaatataaa ggaactacca caatagggtt agtttgcaaa gagggtgtag
     2701 ttttggccac tgagaaaaga gctacgatgg gacattttat tgcaagcaag gatgcaaaga
     2761 aagtctatca gattgatgat ctcgtggcaa tgaccacagc aggctcagta ggagatgcgc
     2821 aaagactcgt gaaatggatg caggttgagt caaaactcta caagatgcga agagaagaac
     2881 ctatgactgt aaaggggata gtaagtttac ttgccaatat cttgagcgga aacagatatt
     2941 acccatatta tgtacagctt cttgtaggcg gcgtcgataa gcatggcccc ggcatttatt
     3001 cactcgatgc ggttggcgga attatcgaag aaaaaaaggc ggtctcaaca ggttccggtt
     3061 ctcctatggc atacggcgtg cttgaggaca gatatgtgga aaacatgcca attgatgatg
     3121 gagttgcact cgccatacgc gcactgcata atgccatgaa
//
LOCUS       DATGBN010000371         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_418716, whole genome shotgun sequence.
ACCESSION   DATGBN010000371 DATGBN010000000
VERSION     DATGBN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418716"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..745)
                     /locus_tag="VKL21_09245"
     CDS             complement(122..745)
                     /locus_tag="VKL21_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HMB45996.1"
                     /translation="MELKDKFTGLWKKYFNNAELPITFYYTDEEGHAELVKPGTVPRC
                     VIGALQKVRKGESYCFDIESIGCFGGKRYLGFAEKIRPNFEYFLSCGIPGEMEGERYK
                     KSPEIVGELMKKQPVFKAPAKYIVFKRWDKLEGSDDPSVVIFFARPDVLSGLFTLSGF
                     DESEPNGVIAPFGAGCASIIQNPYLEKDSDRPRGIIGMFDVSARQIV"
     gene            complement(903..1538)
                     /locus_tag="VKL21_09250"
     CDS             complement(903..1538)
                     /locus_tag="VKL21_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMB45997.1"
                     /translation="MTEINIEKNKILDEIIANRRTIRDFKPDVPPKESIEAILKAGLQ
                     APFAAMAVIGEKRFRRFFVLKEGESMNRLAEIIQERIKVVLDNLSEKDNMHPKAEVFL
                     KVLKDIAVLGFNLRPPYFIIVAEKEGFPPVAQLSLAHCLENMWLKATTLGLGFRLVTL
                     VSELSKDPEFCEFIGIKPGEYAVNGCAIGYAKIIPPKVERPLLNEITTWIN"
     gene            complement(1742..2119)
                     /locus_tag="VKL21_09255"
     CDS             complement(1742..2119)
                     /locus_tag="VKL21_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB45998.1"
                     /translation="MTITSMMMAINDAAQEIPLENSWDGLGLLIFGLPLLSCTVSFLT
                     IYKFTKNITRSALGAIIGFIGAFVGLAIPINTEGDNLQSIINAGGVYAAIIYFVVMTT
                     FIGLIVTSSEKKNKSASNRQYKK"
BASE COUNT          636 a          463 c          360 g          743 t
ORIGIN      
        1 atcaagaggt tctgacatag aattccatag aaaaatacca tttttcatga ttttggcatg
       61 aaaggcagtt cataagggtt tgattgggga gtggaaatca aacagactta aaaaatagtt
      121 tttacacaat ctgacgtgct gagacatcga acatcccgat tattcctctc ggacggtctg
      181 aatccttttc aaggtaagga ttctggatga tcgatgcaca tccggcgcca aatggtgcga
      241 ttactccatt gggctccgat tcatcaaaac cggagagagt aaaaagtcct gagaggacat
      301 cgggtcttgc aaagaaaatt accacgctgg gatcatctga tccctccagc ttatcccatc
      361 tcttaaaaac aatatatttt gcaggcgcct tgaaaacagg ttgctttttc attaattccc
      421 cgacaatctc cggagatttc ttatatcgtt ccccttccat ttccccggga atgccgcatg
      481 agaggaaata ttcgaagttg ggccttatct tttcggcaaa accgagatat ctctttccgc
      541 caaagcagcc gatggattca atatcgaagc aataagattc acctttcctt actttctgca
      601 atgctcctat tacgcacctt gggactgtgc ctggtttaac cagttcagca tgaccttctt
      661 catccgtata ataaaacgta atgggaagtt ctgcgttatt aaaatatttc ttccagagtc
      721 cagtaaattt atctttgagt tccataacca tgactaaatc ctcctgtgta gccagaacca
      781 tatatgttcg tgcggcgagg taattaagat taattatctc tcatcactgt gttaagtagt
      841 tgtcgatgat tttgagtagg cgtttggcaa ccaacaaaat gcataacttc gaaatcattg
      901 atttaattta tccaggtagt tatttcattc agtaatggtc tctctacctt tggaggaatt
      961 attttggcat atccaatggc acatccattt accgcatatt ctccaggttt gatcccgatg
     1021 aattcacaaa attccggatc ttttgacaat tctgatacta aggtcacaag ccgaaatcct
     1081 aatcctaaag tggttgcttt taaccacata ttctcaagac aatgtgctaa tgatagttgt
     1141 gcaaccggag gaaatccttc tttttctgcg acaataataa aatatggcgg tcttaagttg
     1201 aatcctaaaa ccgcgatatc ttttaacact tttaaaaata cttctgcttt aggatgcata
     1261 ttatcttttt cagacaaatt gtccaataca acttttattc tttcttgaat aatctcagca
     1321 agtctgttca tcgattctcc ctccttcaga acgaagaatc ttctgaaccg tttttctcca
     1381 attaccgcca tagcagcaaa aggtgcttgt aaacctgctt tcaagatagc ctctatcgac
     1441 tccttaggcg gtacatctgg tttaaaatcc ctaatcgttc gacgatttgc aattatttca
     1501 tctaaaatct tatttttttc tatatttatt tctgtcattt ctatttccta atttatagat
     1561 aatggattca tcccaacaat gctactaaag ggtctattct cacctgctat tttatatcag
     1621 ttctgttgtt ttcatgacaa gaccagattc gatataaaac ttaaagggct tcaattatag
     1681 cacttccaga taaccataaa ttacgattcc attagcaagc gtattgataa attctttctc
     1741 ttcatttttt atattgtcga ttagatgcag acttattttt cttttcagaa ctggtgacaa
     1801 tcaatcctat aaacgttgtc attactacaa agtaaattat tgctgcatag acgcctcctg
     1861 cgttaataat agattgtaaa ttatctcctt cggtattaat tggaatagcc agcccaacga
     1921 aagcccctat aaatccaatt attgccccca gcgcactacg tgttatattc tttgtaaatt
     1981 tatatattgt aagaaatgat actgtacacg ataataatgg aagaccaaaa attaatagtc
     2041 caagtccgtc ccatgaattt tcaaggggta tctcttgtgc tgcatcattt attgccatca
     2101 tcattgatgt tattgtcatt catttcactt cctgaattgc tgatgctcct tccctgaatg
     2161 ttacatttat tttatttatc tgttccggag ccattatgat tc
//
LOCUS       DATGBN010000372         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_263668, whole genome shotgun sequence.
ACCESSION   DATGBN010000372 DATGBN010000000
VERSION     DATGBN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263668"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /gene="rrf"
                     /locus_tag="VKL21_09260"
     rRNA            <1..92
                     /gene="rrf"
                     /locus_tag="VKL21_09260"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            149..808
                     /locus_tag="VKL21_09265"
     CDS             149..808
                     /locus_tag="VKL21_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HMB45999.1"
                     /translation="MTSELAFFVSVFTAMFSIVNPVSGVMAFISMTSNMKDEDKNYVA
                     KRSVVIACIIAILFTISGNYIMSFFSITVDSLRVAGGILLFLVAMDMLFARTTRESIT
                     SEELSEAVQRENVSIFPIAMPMLTGPGAITTIILYITKGVDEYNISLETVLLFEIAAI
                     LLTFIITYMIFRFSDHFRKVVGMTGMLVMTRLMGLFLGAMAVGFISDGIWNIYKLKLM
                     G"
     gene            809..2296
                     /locus_tag="VKL21_09270"
     CDS             809..2296
                     /locus_tag="VKL21_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HMB46000.1"
                     /translation="MRKMNILVVTGKLASNTVKVSVRNMADILVLDIEVAAFTTPALL
                     WSSLSPKKYDLILIPGLASGDYSGLEKELGIPIRLGPKHAVDLGFVLELAEGFDFSSR
                     VPACELLIEKKRYNAFNRVNELEEKADSPLTLRTVKIGGNSRMKIMAEIVDAGSMNKS
                     DLTDRIIYFVERGADIIDLGISLDTSKSEVRTAVETAKSITNTPLSVDTLDPDSINAA
                     IDAGIDIVLSLNSENISHVNINMARNSTAAVIIPDRIDDLESLFQNMESARILGITNI
                     IADPVLEPAGHGLTKSINRYYEFRKRDITTPLFFGVGNVTELIDADSIGIHSILSSIA
                     MELDASVLFTPEFSHKAHDSVNELKTASIMMMLSRDRKSAPKDLGVDMLVIKEKRRRE
                     FGIIPRSCIEAKGSEKWHLDPAGCFKIETTDYEIRNGKLYPGKIVARNNNNSITGSTA
                     RDILDTIIERGLISRLDHAAYLGRELMKAEIALRIKRSYSQDDKF"
BASE COUNT          753 a          432 c          520 g          699 t
ORIGIN      
        1 gtacccattc cgaacacaga agttaagcct gtcaacgttt tacattgtac taagatgtgc
       61 gaactctcgg gaactgtaga aagtcgctat gctcacttat ttcaactagt ccaaaacatt
      121 caatagtata taatgaaata taatattaat gacatccgaa ctggcattct ttgtttctgt
      181 ttttacggca atgttttcaa tagtgaatcc cgtgagtgga gttatggctt ttatttccat
      241 gacctccaac atgaaagacg aagataagaa ctatgtcgca aaacgttctg tggtcattgc
      301 ttgcataatt gcgatcttat ttacaatatc cggaaactat atcatgagtt ttttcagtat
      361 tactgttgat tctctgaggg tagcaggtgg aattttgctt tttcttgtgg ctatggatat
      421 gctttttgca cggaccacaa gagaaagtat tacatccgaa gaattatctg aggccgttca
      481 aagagaaaat gtttctattt ttccaatagc tatgcccatg cttactggac ctggagccat
      541 aacgaccata atcctatata ttaccaaagg ggttgatgaa tataatatca gtcttgaaac
      601 tgtgttactg tttgaaattg ctgcaatatt gcttacattt ataattactt acatgatctt
      661 tcgtttttca gatcatttta gaaaggtcgt tgggatgaca ggcatgcttg taatgacaag
      721 actgatgggt cttttccttg gggcaatggc agttgggttt atttcagatg ggatatggaa
      781 tatttataaa cttaagttaa tgggataaat gagaaaaatg aatatcctgg tagttaccgg
      841 aaagcttgca agcaatacag taaaggtatc tgtaagaaac atggctgata ttctcgttct
      901 ggatatagaa gtggcagcat ttacaactcc ggcattgctt tggagctctt tatctccaaa
      961 aaaatatgat ctcatcctta tccctggtct ggcatcgggt gattattccg ggcttgaaaa
     1021 agagcttgga ataccaataa gactcggacc aaagcatgct gtggatctcg gttttgttct
     1081 tgaattagca gaaggatttg atttttcatc aagagtaccg gcctgtgagc tcctgattga
     1141 aaaaaagcgt tacaatgctt tcaatagagt taatgaactg gaagaaaagg ccgattcgcc
     1201 tttaacatta agaacagtca agatcggtgg aaattcccgc atgaagataa tggctgaaat
     1261 cgtggatgcg ggttcgatga ataagagcga tcttactgat aggataattt atttcgttga
     1321 acgcggggcg gatattattg acctgggaat ttcattggat acatcaaaaa gtgaggtaag
     1381 gactgctgtt gaaactgcaa aatccataac aaatactccc ctcagtgtcg atacgctcga
     1441 cccggattca ataaatgcag caatagatgc gggaattgat attgttctta gcctgaattc
     1501 agagaatata tctcacgtaa atattaacat ggccaggaac tccaccgccg ctgttataat
     1561 tcctgatcgt attgatgacc ttgaaagtct gttccaaaac atggaatcag cccggatttt
     1621 agggattaca aatattatcg ctgacccggt acttgaacct gcaggtcatg gtcttacaaa
     1681 gtcgatcaac aggtattatg aattcaggaa acgcgacatc acaacgccat tattcttcgg
     1741 ggtgggtaat gtaaccgagc taattgatgc tgattctatt gggatacatt caattctttc
     1801 aagcattgca atggagcttg atgcaagcgt attgtttaca ccggaattca gccacaaggc
     1861 tcatgacagt gtcaacgaat taaagaccgc ttcaataatg atgatgcttt caagagaccg
     1921 aaaaagcgca cccaaggacc ttggagttga catgctggtg ataaaggaaa aacggcgtag
     1981 ggaattcgga ataattccca gatcctgtat agaagcaaaa ggcagtgaaa aatggcatct
     2041 tgaccctgcg gggtgtttta agatcgagac gaccgattat gagatccgca acggaaagtt
     2101 atatcctgga aaaatagtgg cgagaaataa taataattca ataacaggct caacagccag
     2161 ggatattcta gataccataa tcgagcgtgg cctaatttca cgacttgacc atgcagctta
     2221 tctggggaga gaacttatga aagctgaaat cgcattaaga atcaagagga gctattccca
     2281 ggatgacaaa ttttgatatt ataaaaaaaa ttacagaaga tactgatgtt cctgaaattc
     2341 ttgatcgtgc cctgaagaac gaaccacttg acataaatga tggtacgaga cttcttgaat
     2401 gcaa
//
LOCUS       DATGBN010000373         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_418729, whole genome shotgun sequence.
ACCESSION   DATGBN010000373 DATGBN010000000
VERSION     DATGBN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418729"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VKL21_09275"
     CDS             <1..1265
                     /locus_tag="VKL21_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMB46001.1"
                     /translation="IFMLIRISFDFLGASQSWWGYIVLTAGTISAILGILYAVVEPDM
                     KRMLAFSSIENIGIILIGLGASMIFLASGKPILCAIAMIAALYHLLNHSVFKSLLFMG
                     AGSILYSTHTKNIEKLGGLIKKMPMSAIFILIGVLSISAMPPFSGFVSEWLTFQSLLL
                     TFNLNSNFAIIMLSISAALLALTSALAAYCFLKFFGMVFLALPRNENAQHAKEVNIPM
                     LVGMGFLALLSILLGILPAFVLPVLDRIAASFIGIGAFGQSFSYSTFGMISIPTSQSI
                     SISTPGLLAILLILIFIPAIILLINKRNMPLYETWGCGQPASTERNEYSATAFSKPVQ
                     MWFKGLYRPAREMEVTYSSRYLKESFKFESRIEQVFEKYLYIPVVDFVMAISRKAKVI
                     QTGSIHAYLGYIFGTLVILFMFVIAGGS"
     gene            1266..2210
                     /locus_tag="VKL21_09280"
     CDS             1266..2210
                     /locus_tag="VKL21_09280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HMB46002.1"
                     /translation="MIIQLIFQILIIIVLAPLLSGIIKKVKSFFQIRKGPGIMQPYYD
                     ILKLIKKDSVVSNNVSWIFHAAPIISFAAVLAAGLLIPIYITQPPLGFAGDLIAVIYL
                     FALARFFTALASLDAGSSFGGMGGSREMFIAALVEPALMLSIFAVALNVGSTNLGFIS
                     RTVSGMGIDAMSPYQILAFVALFIIAIAETGRIPVDNPATHLELTMIHEAMILEYSGK
                     QLAIVELGAMMKQLLIFSLLANIFFPWGIASEGAGASALVLALLVYMIKILVIGISMA
                     TVEISTAKWRLFRLPELLSISLMLSFLAIVSFIIVKGG"
     gene            2214..2873
                     /locus_tag="VKL21_09285"
     CDS             2214..2873
                     /locus_tag="VKL21_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46003.1"
                     /translation="MSEVVFGSKIMQLLIAFILVSTFMILGSTRLYSCVRAFGIQSFL
                     LACVAGIVAYSTGKNDIYIVALLTLIIKSAVIPYIFIYIIREIKVKREVELYVNISPS
                     IIIGGILVVISYYLIRSISVISELSSLALSASMSLVSIGLFIMISRKKALMQMLGILV
                     MENGLFLGAISLTNGMPLLVELGIFFDVLIGVLIMGILIFRINKTFESIDTDMLKTLI
                     G"
     gene            2878..>3404
                     /locus_tag="VKL21_09290"
     CDS             2878..>3404
                     /locus_tag="VKL21_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46004.1"
                     /translation="MIEYLLVAPILTGILCFFTRSKKQVEAVSLTGSIATLVMGLILV
                     ENVYRNGVIVSWTNALFADAFSAFIVLIVSIVGFVASVYSIGYMGHEFKHGIMDLKKL
                     KIYYVLFHIFMFTMLLVGVTNNLGLWIAIEMTTLVSALLMILYTKKSSVEAAWKYMII
                     CTVGITFALFGTILT"
BASE COUNT          898 a          681 c          699 g         1126 t
ORIGIN      
        1 cgattttcat gctcatcagg atctcatttg atttccttgg agcaagccaa tcatggtggg
       61 gctatattgt gctaaccgca ggcacaatat cggcgatcct tggtatatta tacgcagttg
      121 tggagccgga tatgaaaaga atgcttgcat tcagcagcat agaaaatatc ggcatcatcc
      181 ttattggtct tggcgcttca atgatattct tagcaagcgg gaaacccatt ctttgcgcaa
      241 tagcaatgat cgctgcacta tatcacctgc tgaaccattc tgtttttaaa agtttgctct
      301 tcatgggcgc tggttccata ttatattcaa cccatacaaa aaatatcgag aaactggggg
      361 gtttgatcaa gaaaatgccg atgagcgcca tttttatact aattggcgta ttatcaattt
      421 cagccatgcc gccatttagt ggttttgtga gtgaatggct aacttttcag tctttgctcc
      481 tgacttttaa tttaaatagc aattttgcta ttatcatgtt atccatcagc gcggctcttc
      541 ttgcccttac gagtgcgctc gcagcatatt gtttcctcaa gtttttcggg atggtgttcc
      601 ttgcgctccc tcgaaatgaa aatgcacaac atgcaaaaga agtgaatatt ccaatgcttg
      661 tcggaatggg atttttggca ttattatcaa tattgcttgg tattttgcca gcgttcgtac
      721 ttccagtgct tgataggatc gcagcgtctt tcatagggat tggtgccttt ggccaatcat
      781 tttcatatag tactttcggg atgatctcta tcccaacctc tcagtcgata tcaatttcaa
      841 cacccgggct tcttgcaata ttattaattc ttatattcat tccagcaatt attttattga
      901 tcaataagag aaatatgcca ctctatgaaa cttggggatg cggccaaccc gcctcaacag
      961 aaaggaatga atattcagca accgctttct caaaaccggt acagatgtgg tttaaaggtt
     1021 tatatcgtcc cgcacgagaa atggaagtaa catattcatc ccggtatctt aaggaatcat
     1081 ttaagtttga atccaggata gaacaggtat ttgaaaaata tttatatatt ccggtggttg
     1141 attttgtcat ggccatatcc agaaaggcta aagtgatcca gacaggaagt attcatgcat
     1201 atcttggtta tatctttggg acattggtaa ttctattcat gtttgttata gcaggaggaa
     1261 gctagatgat catacaattg attttccaga tattaattat cattgtcctt gcaccgctgc
     1321 ttagcggaat tataaaaaag gtgaaatctt ttttccagat aagaaaaggt ccgggcatca
     1381 tgcagccgta ttatgatatc ctgaaactca tcaaaaaaga ttctgttgtt tcaaataatg
     1441 tgtcttggat atttcatgcc gcgccaatta tttcctttgc ggccgtactt gccgcaggtc
     1501 ttttgattcc gatatacatc acacaacctc cacttggatt tgccggagac ctgatcgcgg
     1561 ttatttacct ttttgccctt gccaggtttt ttaccgccct tgcttcgctg gatgcgggaa
     1621 gttcttttgg cgggatgggc gggagccgtg agatgttcat tgcggcttta gtggaaccgg
     1681 ctttaatgct ttccatattt gcagttgcgc taaatgtggg gtcaacaaac ctgggtttca
     1741 tttcacggac tgtatcaggc atgggcatcg atgccatgtc tccttaccag atacttgctt
     1801 ttgttgcgct ttttattatc gctattgctg aaacaggacg catccctgta gataatccgg
     1861 ctacacatct tgaacttaca atgatccacg aagcaatgat ccttgaatat tccggaaaac
     1921 aactggcaat agtggaactc ggggcgatga tgaagcaatt gctgattttt tcattgcttg
     1981 cgaacatatt tttcccatgg gggatagcct cagaaggagc aggtgcatca gcattggtcc
     2041 ttgctttgct tgtttatatg ataaaaatcc tggttatcgg aatatcgatg gcaactgttg
     2101 aaatctcaac agcaaaatgg agattattca gattacctga attactgtca atatctctta
     2161 tgctttcatt cctggctatc gtgtcattta ttatagtaaa aggaggataa aaaatgtcag
     2221 aagttgtttt cggatcaaag ataatgcaat tattaatagc attcattctt gtttccacct
     2281 ttatgatcct tggttccaca aggctttatt catgtgtaag ggctttcggt atacagtcct
     2341 ttcttcttgc atgcgtcgct ggcattgtgg catattccac ggggaaaaat gatatttata
     2401 ttgtcgccct cctgaccctt atcataaaat cagcggtcat accatatatt tttatttaca
     2461 tcatccgtga gatcaaagtc aaaagggagg tagaacttta tgtcaatata tcgccttcaa
     2521 taatcattgg cggaatcctt gtagtgattt catattatct tataagatcg atcagcgtca
     2581 tttcagaact ttcaagttta gccctttcag catccatgtc gcttgtgtct atcgggcttt
     2641 tcattatgat tagccggaaa aaagccctga tgcagatgct tggcatactg gtaatggaaa
     2701 atggtctttt tctcggggca atatctttga caaatggaat gcctctcctt gttgaactcg
     2761 gaatattctt cgatgtcctg attggagttc tcattatggg aatcctgatt ttcaggataa
     2821 acaagacatt tgagagcata gatacggaca tgctgaaaac tctgataggt tgatactatg
     2881 attgaatatc ttctcgttgc tccgatattg acaggcattt tatgcttttt tacaagatca
     2941 aagaaacagg tcgaagcggt aagtcttaca ggttctatcg ccacccttgt tatgggtttg
     3001 atccttgtgg aaaatgtata ccgcaacggt gtcatagtat catggacaaa cgcccttttt
     3061 gcagatgcat ttagcgcctt cattgttctt atcgtgtcaa tcgtgggttt tgtagcttcg
     3121 gtttattcaa ttggctacat ggggcatgaa ttcaaacacg ggataatgga tttgaaaaaa
     3181 ttgaaaatat attatgtcct tttccatatc ttcatgttca caatgcttct cgtaggagtc
     3241 accaataacc ttggtctgtg gatcgccatc gagatgacaa cgcttgtttc agcgctcctc
     3301 atgatcctat ataccaaaaa atcctctgtt gaagccgcct ggaaatatat gatcatttgt
     3361 acggtaggta ttacgtttgc cctttttggc acgatcctta ccta
//
LOCUS       DATGBN010000374         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_224505, whole genome shotgun sequence.
ACCESSION   DATGBN010000374 DATGBN010000000
VERSION     DATGBN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224505"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VKL21_09295"
     CDS             <1..269
                     /locus_tag="VKL21_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HMB46005.1"
                     /translation="VQARNADIDWILILGLLIVVITGFYAQTLRGDITRELIEAYPPT
                     FFNNITVLFHEVFTLLFCVAYLPFSKYFHMITAPLTILVNHGGE"
     gene            271..1689
                     /locus_tag="VKL21_09300"
     CDS             271..1689
                     /locus_tag="VKL21_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMB46006.1"
                     /translation="MAKEKPSINTVNFTAAQLMEFDACTRCGECVKYCPTYDARDKNQ
                     DIEPRDKILRWRDFMTKNYGLKARLFGPEKIPPEEIKKFTDDLYNCTTCGMCGTVCPA
                     GINTIELWESTRANLVKYGTGPYGKQSLFPKLIEEGHNPYLKQQKDRLAWMTPDIKIA
                     EKSDIAYFIGCTAGYNQQVLGVSTARILNKLNVPFMVLGEEEWCCGSALIRTGQQHIN
                     DTPKKAAIHNVEALKARCAKRVIFACAGCFRAAKIDWPRAYGKDLPFEAIHMSQFLAE
                     QLEAGKIKWEKSLNKTVTYHDPCHLGRHVGVFEEPRKVIKSMPGIKLVEMKRNRKEQR
                     CCGAGGGVKAGIPDLALGVAKARVTDAKETGAQALISTCPFCRRNLLDGRDELKMDLS
                     VDDLVVLTANLMGLSTDVKPPAPGAPKESISDLFRTQTIPPKPPAEPKKEEPKAAPKA
                     ETPKVEAPKTPTKEAPKASGPK"
     gene            1771..2532
                     /gene="grpE"
                     /locus_tag="VKL21_09305"
     CDS             1771..2532
                     /gene="grpE"
                     /locus_tag="VKL21_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMB46007.1"
                     /translation="MVQENIEEVKKKAEKFETQYNKIKQEFKDYIEISKKNEEKKRLD
                     IRSDISKRLLVVADSLDRMAGSGNASSCELMKSHTENIIKNIDAVYSQMLSASGLTAI
                     EPMAGDKFDEQKHIAIGLEFGVKYPENTVFKVVRKGYLMENNVVRPAEVIISKNPILQ
                     HIAKPGLWDHLLRIIKPEVLNLSGLKQEMDELDRKNKEKMDKLALDIESQNERMDKII
                     KEIESLRIGLQENIAKPEKAKEPQQNQKIDDIPWI"
     gene            2544..>3612
                     /locus_tag="VKL21_09310"
     CDS             2544..>3612
                     /locus_tag="VKL21_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HMB46008.1"
                     /translation="MNKNILGIDFGTTNSKMAYILLDEPVMIENAEGKKITPSIVYFR
                     NEEEVVVGEIAKRNLIINPENTTSSIKREMGTDFKKKIGRHKFPPEYIGAHIFKKLVD
                     DAEKQLGKKFTEAVVSVPANYSDGQRQAIKDAAEIAGINIRRLINEPTAAALAYGIRE
                     DRDKRVLVYDFGGGTFDVSILSVSSGFFDVDSSAGEHKLGGDDIDARIVEHVSKRLYE
                     QMGIDVKKDVALQATLREASEEAKITLSSLESTTINIPFVAANKPPFNMVLTRIELNR
                     MITDLIERTEKPIEQALDDASLDKNEIDDILLVGGTTLIPAVREFVTQYFGKEPLKGD
                     PYEAVALGAAVASMEYGKEKSN"
BASE COUNT         1194 a          703 c          856 g          859 t
ORIGIN      
        1 atgtccaggc ccgcaacgct gatattgact ggatattgat attgggttta ctaattgttg
       61 ttattacagg attttatgcc cagaccctca gaggagatat caccagagaa ttaattgaag
      121 catatccgcc tacatttttc aataacatca ctgttttgtt ccatgaggtc ttcacattgt
      181 tattctgtgt ggcttatctt ccatttagca agtatttcca tatgataaca gcgcccttga
      241 ccatactagt gaaccatggg ggcgagtaaa ttggctaaag agaaaccttc aataaacact
      301 gttaatttca cagctgccca gttgatggaa ttcgatgcat gcacgaggtg tggagagtgc
      361 gtaaagtact gccctacata tgatgcaagg gataagaacc aggatatcga gccaagggat
      421 aagattctga gatggcgaga ttttatgacc aagaactatg gcctgaaggc aaggctcttc
      481 ggacccgaaa agatcccccc cgaggagata aagaagttca cggatgacct ttacaactgc
      541 acaacctgcg gaatgtgcgg aactgtgtgc cctgccggta tcaataccat tgaactctgg
      601 gagtcaacaa gagcgaacct tgtcaagtac ggaacaggtc catatggcaa gcagagcctt
      661 tttcccaaac taatcgaaga aggacataac ccatacctga agcagcaaaa agaccgtctt
      721 gcatggatga cccctgatat caagatagct gaaaaatccg atattgcata tttcatagga
      781 tgcactgcgg gatacaacca gcaggtactc ggtgtcagca ccgcaaggat attgaataaa
      841 ctcaatgtac cattcatggt acttggtgaa gaggaatggt gctgcggttc agctctcata
      901 agaaccggtc agcagcacat aaacgatacc cctaaaaaag cggctattca caatgttgaa
      961 gctttaaagg caagatgcgc aaagagagta atctttgcat gcgcaggatg tttcagggcc
     1021 gcaaagatag actggccgcg tgcatatggg aaagatctgc catttgaggc gatccacatg
     1081 tcccagttcc tggctgaaca gcttgaagca gggaagataa agtgggaaaa gagccttaat
     1141 aagactgtca cttatcatga cccatgccac cttggaagac acgtgggtgt gttcgaagag
     1201 cccaggaaag tcataaagag catgccaggc atcaaacttg tggaaatgaa acgaaaccgc
     1261 aaggaacaga gatgctgcgg cgctggtggt ggcgtaaaag caggtattcc tgaccttgca
     1321 cttggtgttg caaaagcgcg tgtcactgat gcaaaggaaa caggtgcgca agcgttgata
     1381 agcacatgcc ctttttgcag aagaaacctt cttgacggca gagacgaact caaaatggac
     1441 ttgagcgtgg acgaccttgt agttctcaca gctaatttga tgggcctgag caccgatgtc
     1501 aaaccccccg cgccaggcgc tccaaaagaa tcaataagcg acctgttcag gacgcagact
     1561 attccgccca agcccccggc tgaaccaaag aaggaagagc caaaagctgc ccccaaggca
     1621 gaaactccaa aagttgaagc tcccaaaacc ccgacaaaag aagctccaaa agcttcaggg
     1681 ccaaagtagg tattgcctac ttttccttct tttttttata taaatcacct atcggaaaat
     1741 atatccgatt atgcacaaat tgagatttga atggtgcaag agaatataga ggaagtcaaa
     1801 aaaaaggcag agaagttcga gacccaatat aataaaataa aacaggaatt taaagattat
     1861 attgaaatca gcaagaaaaa tgaagagaaa aaaagacttg atatcagatc agatatttca
     1921 aaacgattgc tggtggttgc tgactcgctt gatcgcatgg ctggttccgg aaatgcaagt
     1981 tcatgtgaat tgatgaaaag tcatacggaa aatataataa agaatattga tgctgtctac
     2041 agccagatgt tgtctgcttc ggggttaact gctatcgaac ccatggcggg tgataaattc
     2101 gatgagcaga agcatattgc tatcggactt gaatttggtg taaaatatcc tgagaacacg
     2161 gtcttcaagg ttgtaagaaa aggatatcta atggagaata atgtggttcg tccggctgaa
     2221 gtgataatct caaagaaccc aattttgcag cacattgcga aaccgggatt atgggatcat
     2281 ctgttaagga tcataaaacc tgaggttttg aatttatcag ggctaaagca ggaaatggat
     2341 gaacttgatc gaaagaataa ggaaaagatg gacaaattag cgcttgatat tgaatcgcag
     2401 aatgaaagaa tggataaaat tattaaggaa atagagtctc tcaggattgg gttgcaagaa
     2461 aatattgcaa aacctgaaaa agcaaaagag ccgcagcaaa atcaaaaaat cgatgatatc
     2521 ccatggatat gaggtagatt acattgaata aaaatatact tggcattgac tttggtacca
     2581 ccaactcaaa aatggcatac atactgcttg atgagcctgt aatgatcgag aacgccgaag
     2641 gaaaaaagat cacaccttca attgtttatt tcaggaatga agaggaagtc gttgtgggtg
     2701 aaatagccaa gcgcaacctg atcataaacc ctgaaaatac cacatcatcg atcaaaaggg
     2761 aaatgggaac agattttaag aaaaagatcg gacgtcataa atttcctcct gaatacatcg
     2821 gagctcatat ttttaagaaa ctggtggacg atgctgaaaa acagcttggt aagaaattca
     2881 ctgaagcagt agtatctgtt ccagccaatt attcggacgg acaaaggcag gctattaagg
     2941 atgctgctga aattgctggc ataaatatcc gaagattaat caatgaaccc actgctgcgg
     3001 cccttgcata cggtataagg gaggacaggg acaaaagggt gcttgtgtat gacttcggag
     3061 gcgggacatt cgatgtatca atactttctg tttcatcagg tttctttgat gtggattcaa
     3121 gcgcgggaga acacaaattg ggcggagatg acattgatgc gcgaattgtt gaacacgtga
     3181 gcaagagatt atacgaacaa atgggtatcg atgttaaaaa agacgttgct cttcaagcga
     3241 cgctgcgcga agcgtctgag gaagcaaaaa ttaccctatc atctttagaa agtacaacta
     3301 tcaacattcc ttttgtggct gcaaacaaac ctccattcaa tatggttttg acaaggattg
     3361 aattgaacag gatgattaca gatcttatcg agcgtacaga aaaaccaata gaacaggctc
     3421 ttgatgatgc ttctctagat aagaacgaga tagacgatat attgctcgtg ggaggcacga
     3481 cgctgatacc ggcagtccgt gaatttgtaa cgcagtattt tggcaaagaa cccctgaagg
     3541 gagatcctta cgaggctgtg gcgcttggtg ctgccgttgc atccatggaa tatggaaaag
     3601 aaaaatcgaa ta
//
LOCUS       DATGBN010000375         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_496037, whole genome shotgun sequence.
ACCESSION   DATGBN010000375 DATGBN010000000
VERSION     DATGBN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496037"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VKL21_09315"
     CDS             <1..213
                     /locus_tag="VKL21_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HMB46009.1"
                     /translation="TASTGSACSSKTLEPSHVLIAMGLRHEEAHGSLLLTLGKGNTIE
                     DVDHVIVSVPKIVARLRELSPLYVRK"
     gene            215..1549
                     /gene="nifU"
                     /locus_tag="VKL21_09320"
     CDS             215..1549
                     /gene="nifU"
                     /locus_tag="VKL21_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HMB46010.1"
                     /translation="MEEQFEYSEKVMDHFRNPRNVGQVKDADGIGRVGNPVCGDLMEI
                     QIKVENNILKDVKFKTFGCGSAIATSSMITEMAIGKTLEEALKITRGEVAEELGGLPL
                     IKMHCSNLAADALHAAIKDYMSHKEEKEKIEAQKYDFDVIIVGGGPGGLTTGILCAYR
                     GLKTAIFEASNWGGILSWLCPNKMIENFPGLCEKSTCSDLVNSWVNEAKKLKVEMKKE
                     RVNEITPDKKIISESGEYKGKIIVLATGSSPAPGGIKGEEKFGKNERGVYYYVRNPGN
                     FTGKRVIIVGGGNSAVDAALSLADTADLITIACKNEELRSVPNKIERLKAIDRVKVLY
                     NTEVLEISGTDKVEKVLMKDLKEDENIQQVVDSVVLAVGLTPNTEIFKNLGLEMDDKG
                     YLKTDKTQKTNIDGIYGVGDIASDLQLVVVAVSNGATVAHNAYIELRKPYWR"
     gene            1616..2524
                     /gene="argF"
                     /locus_tag="VKL21_09325"
     CDS             1616..2524
                     /gene="argF"
                     /locus_tag="VKL21_09325"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HMB46011.1"
                     /translation="MPSHLLSLADLTYSDIINLLDCASHLKEKRTRGKSSEDLKNKTL
                     AMLFEKSSTRTRLSFEAGMTELGGHAIYLNYKDVQLGRGESVADTARVMSRYVHAIMA
                     RVNKHETLIQLSENGTVPIINGLSDLEHPCQLLADLLTIREYKGKFKDLNFAWIGDGN
                     NVCNSAMLACALTGMKMNVACPQGYEPNAEILTKSRELGGMINVVTDPIKAAKNADIL
                     STDVWVSMGDEEEYDRRLCDFKPYQINSKLLEQAKHDVMVLHCLPAHRGEEITAEVVD
                     GPNSAVFDQAENRLHAQKALLLKLLS"
     gene            2536..2620
                     /locus_tag="VKL21_09330"
     tRNA            2536..2620
                     /locus_tag="VKL21_09330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2572..2574,aa:Leu,seq:cag)
     gene            2684..>4409
                     /locus_tag="VKL21_09335"
     CDS             2684..>4409
                     /locus_tag="VKL21_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HMB46012.1"
                     /translation="MGEKKNLLINIKKVSFPNLSRHFYILLISIFFIQFLCIPAFAQS
                     VAYNEGYNEITYDNGSKDIINGALNFLRYDGAWRPNEELNISNGSWPYLYTQNATTAD
                     FKVDDTTLSIPKTNTLFKTNLNSISYELKYTKSELQGGKGMSIRSNRANIDIPYHFKS
                     RKPHKEFRDKNNIRYGRFNFRAGSEHIVVHDDTPRDYWEDGKVFQDVTYLFQPDYEFA
                     LENGTIRLVFNNNSLDKLTGNVIIEIRTWDIIGTNNWGGNVAFSQTSDVKATGNVELK
                     QKVTNYALYTRFDEGSGKIIHNENPSNVRLGDLLGAVGTGTYTAGKYGLAIHFNGIDN
                     KVLFKDHPDFRLPGDFTISYYIKLTSDVSNADSDITRKGSTATANPDSWWKVEITKNI
                     MHGSVTKNGGKAVESYDTKDRRDGLWHFVAYTRGGTTCSLIVDGTTVKTTSNCPTNAV
                     NTALLSIGAKDTYVQTTGLDFTHGTIDEVRIFNRKLSSSELTSIRNNAHYTTGTVTRN
                     LVSVIKTGEEIKALGCYGTWDSSITKVDIKASADNNNWITVKSNAAPNVNYAVTPGNS
                     YKYSRCSLS"
BASE COUNT         1436 a          820 c         1045 g         1108 t
ORIGIN      
        1 acagcaagca caggttctgc atgttcctcg aaaacgcttg agccctcgca tgtcctgatc
       61 gcaatgggat taaggcatga ggaagctcat ggctcgctat tgcttactct tggaaagggt
      121 aacaccatag aagatgtgga ccatgtcatt gtatcggtcc cgaagatcgt tgcacgatta
      181 agggaattgt cgccgcttta cgtgaggaaa taatatggaa gaacaattcg aatattcaga
      241 aaaggtaatg gatcatttca ggaacccaag gaatgtaggc caggtaaagg acgcagatgg
      301 cattggaagg gtgggaaatc cagtgtgcgg agacttgatg gagatccaga tcaaagtaga
      361 gaacaatatc ttaaaagatg tgaaattcaa gactttcggc tgcggatcgg ctatcgcgac
      421 aagcagcatg ataacggaaa tggctatagg gaaaacgctt gaagaagcgc tcaagatcac
      481 gcgcggtgag gtggccgagg aacttggggg tttacctctc atcaagatgc actgttccaa
      541 tcttgctgcg gatgcgctcc atgcggctat caaggattac atgtctcaca aggaggagaa
      601 ggaaaaaatt gaagcgcaaa aatatgattt cgatgtgatc atcgtaggtg gagggccggg
      661 cggactgacc acggggatcc tttgcgcgta ccgcgggctc aagacagcga tctttgaagc
      721 ttcgaactgg ggcggcatcc tgtcttggct gtgccccaat aagatgatcg agaattttcc
      781 ggggctatgt gagaaatcaa catgctcaga ccttgtaaat agctgggtga atgaagcaaa
      841 gaaattaaaa gtggaaatga aaaaagaacg tgtgaatgag atcacacctg ataagaaaat
      901 catttcagaa agcggggaat ataaaggtaa gatcattgtt cttgcaacag gcagttctcc
      961 tgcgcccggc ggtataaaag gggaagagaa attcggcaag aatgaaaggg gagtatatta
     1021 ttacgtaagg aatcccggaa atttcacagg caagcgtgtc atcatagtcg ggggagggaa
     1081 ttcagcagtg gatgcagcct taagcctggc tgatactgcc gacctcatta cgatcgcatg
     1141 caagaatgag gaattaaggt cagtcccgaa taaaatagag cgcctgaaag ccatcgacag
     1201 ggtcaaagta ctgtataata ccgaagtcct tgaaatttca ggtaccgata aggtcgaaaa
     1261 agtcctgatg aaagacctga aagaagatga aaacatccag caggtcgtgg attctgtcgt
     1321 actggcagtg ggcctgacac ccaataccga aattttcaag aatcttgggc ttgaaatgga
     1381 tgataaaggc taccttaaga cggataagac ccagaagacc aatatcgatg gcatttatgg
     1441 tgtcggggat atcgcttctg acctccagct ggttgtggtt gccgtctcga atggggcaac
     1501 agtcgctcat aatgcatata ttgaattgag gaaaccctat tggagataag gaaaagaaat
     1561 ttaaataaga ttctacaatt agaaactcat aataattaac agataaggag ttcttttgcc
     1621 atctcatcta ttatcccttg cagatttaac gtattccgat atcattaatt tacttgattg
     1681 cgcatcccat ctaaaagaaa aacggacccg gggaaaatca tcagaagatc tcaagaataa
     1741 aacccttgcc atgctttttg agaaatcctc aacacgcaca aggttatcat tcgaagcagg
     1801 gatgacagaa cttggcgggc atgcgatata tcttaattat aaggatgtcc agttagggcg
     1861 cggggaatcg gtggctgata ccgcgcgcgt gatgtcgcgg tatgtccatg cgataatggc
     1921 gcgggtgaat aagcatgaaa cactcatcca gctttcagaa aatggtactg tgcctattat
     1981 taacggttta tctgacctcg aacacccatg tcagcttctt gctgaccttc ttacaataag
     2041 ggaatacaaa ggaaaattta aagatcttaa ttttgcatgg ataggggatg gcaataatgt
     2101 atgcaattcc gccatgcttg cctgcgcact caccggaatg aaaatgaatg ttgcgtgtcc
     2161 gcaaggatat gaacccaacg ccgaaatcct cacgaaatcc agggaactgg gaggaatgat
     2221 caatgtagtc acggatccga taaaagcggc aaagaatgct gatattctct caactgatgt
     2281 ctgggtctca atgggtgatg aggaagaata tgaccgaagg ttatgcgact tcaaacccta
     2341 ccagatcaac agcaagcttt tagaacaagc caagcatgat gtgatggtgc ttcactgtct
     2401 tcccgcccac aggggcgagg agatcacagc agaagttgtt gacgggccaa actcggcagt
     2461 atttgaccag gctgagaaca ggctgcatgc ccagaaggca ttgctcctga agctgctgtc
     2521 ataataaagc aaattgcgag ggttgcccag accggtcaaa ggcgatagct tcaggggtta
     2581 tttccgaagg gattcgaggg ttcaaatccc tcccctcgca tttcaacaaa tatttaaaga
     2641 ataagatgct ataaggttgc atatcctgtt gtggtgattt attatgggag aaaaaaaaaa
     2701 tctgttgatc aatatcaaaa aagtatcgtt tccgaatctt tcaagacatt tttacatact
     2761 tctaataagt atttttttta ttcaatttct ctgtatccct gcattcgccc agagtgtggc
     2821 atataacgaa ggatataatg aaataactta cgacaatggg tcaaaggata tcataaatgg
     2881 ggctttgaat tttcttcgat atgatggggc atggagacca aacgaagaat tgaatatttc
     2941 caatggaagc tggccttact tatatactca aaatgcaacg actgctgatt ttaaggtaga
     3001 tgatactacc ctgagtattc caaaaacaaa tacgttattt aaaacgaatt taaattccat
     3061 cagttatgag ctaaaatata caaaatcgga attgcagggg gggaaaggaa tgtccattag
     3121 atcaaatcgc gcaaatattg atattccgta tcattttaaa tccaggaaac ctcacaaaga
     3181 gtttagagat aaaaataata ttagatacgg cagatttaat tttagagcag gatcggaaca
     3241 tatagtagtc catgacgaca cacctcgtga ttattgggag gatggaaaag tattccagga
     3301 cgtaacatat cttttccaac ctgattatga attcgcgtta gaaaatggaa caattcgatt
     3361 agtatttaat aataattcac tcgataaatt gacgggaaat gtcattattg agatcaggac
     3421 atgggacata attggcacca acaattgggg cggaaatgta gcattttctc aaacttcaga
     3481 cgttaaagct accggcaatg tggaactcaa acaaaaggtt actaattatg cattatacac
     3541 caggttcgat gaaggaagtg gaaaaataat tcataatgaa aatccttcta atgttcgcct
     3601 gggtgatctt ttgggcgctg ttggaactgg tacatataca gctggtaaat atggactggc
     3661 tattcatttt aatgggatag ataataaagt attgttcaaa gatcaccccg acttccggtt
     3721 gccgggtgat tttacgattt catattacat taaattgact tcagatgtga gcaatgctga
     3781 ttcggatata acaagaaaag gcagcactgc cactgcgaat ccggactcat ggtggaaagt
     3841 cgagattaca aaaaatataa tgcacggctc cgtaacaaaa aatggtggca aagcagttga
     3901 gtcatacgat acaaaagatc gaagggatgg attgtggcat tttgtcgcat ataccagagg
     3961 agggacaaca tgcagtttga tagtagacgg cactactgtc aaaacaacaa gcaactgccc
     4021 cacaaatgcc gtaaatactg ctttgttgag cataggggcg aaagatacct atgtgcaaac
     4081 cacaggattg gatttcactc atggaactat agatgaggtg agaatattca atcgtaaatt
     4141 atcatcctcg gaacttacat ctatacggaa taatgcacat tatacaacag gtactgtcac
     4201 ccgtaacctt gtttccgtga tcaaaacagg agaggaaata aaagcgttag gatgctatgg
     4261 aacatgggat agttcaataa cgaaagtaga tattaaggca agtgccgata ataataattg
     4321 gattacggta aaatcaaatg cagctccgaa tgtaaattat gctgttactc cagggaacag
     4381 ttataagtat tcaagatgct cgctaagta
//
LOCUS       DATGBN010000376         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_147252, whole genome shotgun sequence.
ACCESSION   DATGBN010000376 DATGBN010000000
VERSION     DATGBN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147252"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VKL21_09340"
     CDS             complement(<1..195)
                     /locus_tag="VKL21_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46013.1"
                     /translation="MSEIVNFLIEPATQAIFWLICIIFAILFARFVKKLSFGDDTGTR
                     AWEIIALGLFLIGLRVSFKLI"
     gene            complement(196..438)
                     /locus_tag="VKL21_09345"
     CDS             complement(196..438)
                     /locus_tag="VKL21_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46014.1"
                     /translation="MEKEKYLKIALENLLKVFSEGGAKATIDVMAKLKIGAKTDVDPN
                     LMNDCNSVLYERVKMLKGDASAVQFLSSIKTACGGN"
     gene            736..1416
                     /locus_tag="VKL21_09350"
     CDS             736..1416
                     /locus_tag="VKL21_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMB46015.1"
                     /translation="MTIEKGDFIRVSYTGKTDEDRVFDTTDEDVAKANSIYNEKGKYG
                     GDVVIVGAKHTVAGLDEDFVGKEIGYTGSVTIPPEKAFGPRNPELIESIPITKFNQKI
                     QVGMPVEVDGRPGIVIRAIGRMAQVDFNRFLAGQTVTYDYEIKEKIDDVEGKVKGLLG
                     LYIGKDLPVRVNDDNITVDIEPEMTYNQRWLMSKRQIAREVIDNTGIKEIIYLEKYNK
                     EILEPKTE"
     gene            complement(1417..>2321)
                     /locus_tag="VKL21_09355"
     CDS             complement(1417..>2321)
                     /locus_tag="VKL21_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46016.1"
                     /translation="FSDAFIAGASILRTEIEIRDRILLNDILLALHSNTIIVEGLDFQ
                     SFVVKKVKELRKKLNPDINIEYYPHFKRYTLLRLKDFIPKLDQKGLKEHYGEIITRFL
                     SGETSIRRLHSKEIAEKLSNDLSYYDEDLFLASTEGVFILGCEDYIRDLRELLELAIS
                     LLLLFQLYDQKIDVEITNAFDAIKKLSSSRYYFLTQVPRKLEKSLLKVSEIGLVILDD
                     IEDLMNPQKITQDWYYQSAYQKMLNTLKVGDFEKVVQHKIDSLQDLYITARELSSEQL
                     NILLEVAIVILILWEVVKPALVPG"
BASE COUNT          739 a          448 c          446 g          688 t
ORIGIN      
        1 tatcagcttg aaactcactc gaagacctat gagaaatagt cctagggcta taatctccca
       61 tgctcttgtt cccgtatcgt ccccgaaact taattttttc acgaaccttg caaacaatat
      121 ggcaaatata atgcatataa gccagaatat tgcctgtgtt gctggttcta tcaggaaatt
      181 tacaatttcg ctcatttaat ttcccccgca tgcggttttt attgaactga ggaattgcac
      241 cgcgcttgca tctcctttta acatttttac cctttcatat aagacagaat tgcaatcatt
      301 catcagattt ggatcgacat ctgtttttgc acctatcttt aattttgcca tcacatcgat
      361 tgtggctttt gctcctcctt ctgaaaagac ctttaaaagg ttctcaagag caatttttaa
      421 gtatttttct ttttccacag tatcaccttg ttttcatcgt tttatgtaga tttattacat
      481 aaattataaa ttaattcttt tagaaagtat atataacttt gcatccgata atcataacta
      541 taaccataaa aaaatacaca cattagagaa aatgataatt gaaccataat cttcaatacc
      601 tgcaacgact ggtttttcat gtttccaaaa tgcgatataa atcagaaacg accttcacag
      661 atttttcatt tgtcaagtaa gtaagtattt tataccatcg atgcatgaat ggcgcaaaaa
      721 aggaaatgaa tgattatgac tattgaaaaa ggcgatttta taagagtttc atataccgga
      781 aaaactgatg aagaccgcgt ttttgacaca actgatgaag acgtggcaaa agccaacagc
      841 atctataatg aaaagggaaa atacggtgga gatgttgtaa tagtaggcgc aaagcacacg
      901 gtcgccggat tggatgagga ttttgtggga aaggaaattg gatacaccgg cagcgtaacc
      961 atccctccgg aaaaagcctt cggtccaagg aatcctgaac ttattgaaag cataccgatc
     1021 acaaaattca accaaaaaat ccaggttgga atgcctgtag aggttgacgg ccgccccgga
     1081 atagtaataa gagcgatcgg aaggatggcg caggtggact ttaacaggtt cctcgcaggg
     1141 cagacggtaa catacgacta tgaaataaaa gagaaaattg acgatgttga aggcaaggta
     1201 aaaggtctcc ttggattgta tatcggaaaa gatttgccag tccgggtaaa tgatgacaac
     1261 ataacagttg acatcgaacc agaaatgaca tataaccaga gatggctcat gtccaaaagg
     1321 cagatcgctc gtgaagtgat cgataacaca ggcatcaaag aaataattta tcttgaaaaa
     1381 tataacaagg aaatactgga gccaaaaacc gaataattat cctggaacaa gggctggttt
     1441 aacaacttcc cacaggatca aaatgacgat agcgacttca agcaatatat tcaattgttc
     1501 ggatgaaagc tctcttgcag ttatataaag gtcttgcagc gaatcgattt tatgttgtac
     1561 tactttctca aagtccccca ctttgagagt gttcagcatc ttctggtagg ctgactggta
     1621 gtaccagtcc tgcgtaattt tctggggatt catgaggtct tcgatatcat caaggataac
     1681 aagccctatc tcgcttactt tcaacaggga tttttcaagc tttcttggaa cctgcgtaag
     1741 gaaataatac cttgaactgc taagtttttt tattgcatca aaagcatttg tgatctcaac
     1801 atctatcttc tggtcgtaaa gctggaacaa aaggagaagt gagatggcaa gttcaagcag
     1861 ttcgcgcaga tccctgatat aatcctcgca tcccagtata aatacccctt ccgttgaagc
     1921 aaggaaaagg tcttcatcgt aatatgagag gtcattggat agtttttcag cgatctcttt
     1981 gctgtgaagc ctgcggatgg atgtctctcc tgaaaggaaa cgcgttatta tctcaccata
     2041 atgttctttc aggcctttct ggtcaagctt tgggatgaaa tccttaagtc taagaagcgt
     2101 gtatctttta aaatgtgggt aatattcaat attaatatca ggattcagtt ttttgcgcag
     2161 ttcttttact ttctttacta caaatgactg aaaatcaagc ccttccacga ttatcgtgtt
     2221 tgaatgaaga gctaacagaa tatcatttaa caatatccgg tcccttatct ctatttctgt
     2281 acgaagtatc gaagcgcctg ctataaaagc atctgaaaat a
//
LOCUS       DATGBN010000377         6816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_30032, whole genome shotgun sequence.
ACCESSION   DATGBN010000377 DATGBN010000000
VERSION     DATGBN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30032"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VKL21_09360"
     CDS             <1..650
                     /locus_tag="VKL21_09360"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB46017.1"
                     /translation="GTLGIDLGIVYLATDNDGKTFSGKQIDKVREKNAILRINLQTAG
                     TISAKRHLKRFSNREARFHRDINHIISRKIVAKAKGTKQAIALEDLKGIRKSVTVRRT
                     QRSRLHSWAFYQLISFIDYKAALVGIPVFLVNPGGTSHICPECKHNERANRPNRDTFR
                     CVQCGFSGNADHIAAINIAARAVEVNHRIVACDEVKALIGIEMEHSDKPHSFSVR"
     gene            668..1456
                     /gene="dph5"
                     /locus_tag="VKL21_09365"
     CDS             668..1456
                     /gene="dph5"
                     /locus_tag="VKL21_09365"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HMB46018.1"
                     /translation="MLTFIGLGLYDEKDITVKGLEAVKNADAVYVEFYTSNLIGTSIE
                     KIEKFYGKKLIVLSREEVELEPVWLSRSIKENIVFLSGGDAMVATTHIDLRLRAKDMG
                     VKTAIIHAPSISSAVSGLSGLSNYRFGKSTTIPFPYIRNEKTIISEAPYDTIKMNRKN
                     DLHTLVFLDIDREKGFMDIRKAALLLLEMEGRRGEGLLVNSLGVGIARAGSPSPIVHS
                     DYLERLKDHDFGGPLHILLIPASLHFIEAEALVKFCGAPPEILK"
     gene            1746..3290
                     /locus_tag="VKL21_09370"
     CDS             1746..3290
                     /locus_tag="VKL21_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HMB46019.1"
                     /translation="MFSNKICRDTLGITIGFLIITLFIVQGASASTLGANTNDHSKNN
                     VKAADRYNVDPGSPTINWKTKASMPTSRSDLYRGRMAIGDKIYVAGGWKNGSLDVVEV
                     YDVSKNKWNTVAPLPSPGINGAIQAVNGKLYIIGGNKNVGGDVDVWEYDPVKNIYSKK
                     ASFSGENDPATAVVNGKIYAFSGGISESGVKIVAQMYDPINDTWIPKAPHPLQRQYLT
                     AEAVNGKIYTFGGNKNSNLTHEYDPITDNWTEKSPMPVWVDSPNSVVVDGKIFVVGGD
                     SSKTVWMYDPLKDLWQGPLDYEIPTPRALAATAEVNGKIYVIGGCCPAVNSNANEEGT
                     VNFTIARNITSQNATRKIEKESLRLGESTNITIRINSTVIQALALQESIPAGWNFSII
                     SDDADDFNNNSNEWIWLNVTPGITRTVIYTITAPSDASIGTYYINGTISNSSGVIGIV
                     GENNMITLDIFEYYRRLGSNPDILETSDLLKAIDDWGSGTAPVGFVSPVTYNEINALI
                     NEWATK"
     gene            3403..4740
                     /gene="purB"
                     /locus_tag="VKL21_09375"
     CDS             3403..4740
                     /gene="purB"
                     /locus_tag="VKL21_09375"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMB46020.1"
                     /translation="MAIHPIEYRYGHDEMKAVWNEETRLLKMLSVEEALAKSQVRLNY
                     IPKGMDEKISLGVKLVRLNRVKEIEDEIHHDVMAVVLALAEQSQDAGKWIHFGATSND
                     ILDTATALQIKDALFILRKETIKLRQVLIESALSHKNTVCAGRTHGQIGVPTTYGLRF
                     AIWASEIDRHIERLDQLMPRATVGKMSGAVGTQAAFGEKGIEIQKRTMEYLGIEAADV
                     SNQIIQRDRHAEFVMWMANTVTTLDKICIEIRSLARSEIAEVEESFGKKQVGSSTMPH
                     KRNPIKSEQVCGLARIVRAMVEPELRNNTLWDERDLTNSSCERIVFPESCVLTDHVIR
                     LTTTIIQNLRFYPENIRKNLDLLNGLNMGEAIMIELSKKGVGRQEAHEIVRQCAMSAR
                     ESGVHMKDALLKNETVSKYMNESEIIRIMNPDNYIGTAVGQVDSLAAKLRKRK"
     gene            4754..5206
                     /locus_tag="VKL21_09380"
     CDS             4754..5206
                     /locus_tag="VKL21_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HMB46021.1"
                     /translation="MLRKATIKDVEKIWKLVNSYADKRIMLPRSLSELYENLRDFYVV
                     IEDNKLLGCGALHITWEDYGEILSLAVEPAMVRQGIGSQILKACEEEAITLGLNKLIT
                     LTYAPEFFESHGFVRVKKSTLPHKIWSMCIKCPKFPECDEIPLVKKII"
     gene            5325..5972
                     /locus_tag="VKL21_09385"
     CDS             5325..5972
                     /locus_tag="VKL21_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46022.1"
                     /translation="MPYAAKDRQEFIDRSNLKPDSHILDFGGMLIEELTSAVYGITSK
                     RSLSNAVLIRPLLLPFKNSVFRSVISYHYFDLVSSDKLGFAFEEASRVLDKDGSFSFM
                     ILQWTANNEAQKSNLLFNEVLKSIGVLYQHDLEEISMNLNKSGFTEITVESIKREIPI
                     PQEFINEHLLMLGNLVKIEKKKDGMEIKALAKQYFHHIKEHGEALLPAIHFIAKK"
     gene            5974..6573
                     /locus_tag="VKL21_09390"
     CDS             5974..6573
                     /locus_tag="VKL21_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HMB46023.1"
                     /translation="MKKIHLEMVLEKVEGFKSPSACKEQYATPATVASELLYFAFMRG
                     DLVDTVYDLGCGSGIFAIGAKLLGAEKVIGFDDDKDVLEIARANAKKLGVEVEFVCCN
                     ISDVSGKAHTVVMNPPFGAQKKGSDRPFLRKALELSNAVYSIHNAGSAEFIKKFISPS
                     VITEYRLIDFPIKRTFGFHKKERLVIKVEIYRIEKRIEG"
BASE COUNT         2231 a         1220 c         1538 g         1827 t
ORIGIN      
        1 ttggaactct tggcattgat ttgggaattg tgtatcttgc tactgataat gacggtaaga
       61 ctttcagtgg aaagcagatt gataaggtca gggagaaaaa tgccatcctt agaatcaacc
      121 ttcaaacagc aggaactata tccgcaaaga ggcatctcaa aagattttcc aatagagaag
      181 ctcgtttcca tcgtgacatt aatcatatta tttccaggaa gatcgtggct aaagccaaag
      241 gcacaaaaca agcgatagct cttgaagacc tcaagggaat tcgtaagtct gtaacggtaa
      301 gaaggactca gagaagccgg cttcattcct gggcgttcta tcagttaata agtttcatcg
      361 actataaggc tgcccttgtt ggtatccctg tatttcttgt taatccggga gggacttctc
      421 atatctgtcc tgaatgcaaa cataatgaaa gagcgaatag accaaatcga gatacattta
      481 gatgtgttca gtgtggcttc tctggcaatg ctgaccacat agcagcgatc aatattgctg
      541 caagagccgt tgaagtcaat caccggatag ttgcatgtga tgaagtcaaa gccctaatag
      601 gaattgagat ggaacatagc gacaagcctc acagctttag cgtgaggtaa ttgactgtaa
      661 aggaattatg ctcacattta tcggacttgg tctttatgat gaaaaggata ttacagtaaa
      721 aggattggaa gcagtaaaaa atgctgatgc tgtctatgtt gaattttaca catcaaatct
      781 tatcggaacc tccattgaaa agatcgaaaa attttatgga aagaagttaa tagtattgtc
      841 aagagaagaa gttgaacttg aacccgtatg gttatcacgt tcaataaaag aaaatatagt
      901 attcttaagc ggaggtgatg ccatggtcgc cacaacgcat atagaccttc ggctgcgggc
      961 aaaagacatg ggtgtcaaaa ctgcgattat ccatgcgcct tccatatcct cagctgtttc
     1021 gggattgagc ggtctttcga attacaggtt cggaaaatcc actaccatcc ctttcccata
     1081 tataagaaat gaaaagacga tcatttccga agcgccgtat gataccataa aaatgaacag
     1141 gaaaaatgac ttacacactc ttgtttttct tgatattgac cgggaaaaag gctttatgga
     1201 tataagaaaa gctgctttgt tgcttcttga aatggaagga agaaggggtg aaggattgct
     1261 cgtgaattct ctcggagttg gtatagcacg agcaggatct ccatcaccca tcgttcattc
     1321 ggattatctt gagagattga aagatcatga ttttggaggg ccgctgcata ttctcctgat
     1381 ccctgccagt cttcacttta tcgaagccga agctcttgta aaattttgcg gcgcccctcc
     1441 tgaaattcta aaataggatt attttaattt tcttttttct tttgcaagtt tcttcttagc
     1501 ttcaccactt cctgatgctg ccattttttc aagttctgtg attttttcct tgcgatcacg
     1561 ttctttacgc tccataatct ttttagacat tgccatgata tatatttccg ttgtttaaag
     1621 tatttaaatc catccgaaat tcaatacttt tgcgttagat atatgtatat tcgggtcaat
     1681 cttactattc gtgtgtttat cttcatgctc atattgtctt gaaatattca taaggagaat
     1741 ggaaaatgtt ttccaataaa atttgtagag ataccctagg aataacaatt ggatttttga
     1801 taattacatt atttattgtt caaggtgcca gtgcatcaac gcttggcgca aacacaaatg
     1861 atcattccaa aaacaatgta aaggccgcag ataggtataa tgttgatccg ggatcaccaa
     1921 ctatcaactg gaaaacgaaa gccagtatgc ctacttccag atcagattta tacaggggaa
     1981 gaatggccat aggtgacaaa atttacgttg caggaggatg gaaaaacggt tctcttgatg
     2041 tagttgaagt ctatgatgta agcaaaaata aatggaacac ggtagcacct ttaccatctc
     2101 ctggaataaa cggagctatt caggcagtaa atggaaaatt atatattatc ggtggaaaca
     2161 aaaatgttgg gggagatgtt gatgtctggg aatatgatcc tgttaaaaat atttactcaa
     2221 aaaaagcctc tttcagcgga gagaacgatc ctgctactgc agtagttaac ggtaaaatct
     2281 atgcatttag tggtggcatt tctgaaagcg gcgtgaaaat agtcgcgcaa atgtacgatc
     2341 ccattaatga tacatggata cctaaagcac cccatccttt acaacggcaa tatcttactg
     2401 ccgaggcggt taacggtaag atttatacat ttggtgggaa caagaattcc aatttaacgc
     2461 atgaatatga tcccatcact gataattgga ccgaaaagtc cccaatgcca gtttgggtag
     2521 atagcccaaa ttccgtagtt gtcgatggaa aaatattcgt agtaggtggg gatagttcaa
     2581 aaactgtctg gatgtacgac cccctgaagg atctttggca gggaccgctg gattatgaaa
     2641 tacccacacc cagagcgctt gctgcaacag ccgaagttaa tggcaagata tatgtcattg
     2701 gaggatgttg ccctgcggta aattctaatg ccaatgaaga aggaactgta aactttacaa
     2761 ttgccaggaa cataacatca caaaatgcaa cacgtaaaat cgagaaggaa tctctcagat
     2821 taggtgaatc aaccaatata accatcagga tcaatagcac tgtaattcaa gcattagctc
     2881 ttcaagagtc aattccagca ggatggaatt tttcaataat ttctgatgat gctgatgatt
     2941 tcaataataa ctctaatgaa tggatatggc taaatgtaac gccggggata accagaacgg
     3001 ttatctatac cataacagca ccttctgatg cttctattgg aacgtattac attaacggga
     3061 caataagcaa ctcaagtgga gttattggaa ttgtcggtga aaataatatg attacacttg
     3121 atatttttga atattataga agacttggga gtaatcctga tatattagag acctcagatt
     3181 tgctaaaagc cattgatgat tggggaagcg ggacagcgcc tgttggattt gtaagtcctg
     3241 taacatacaa tgagattaat gctttgatta atgaatgggc cacgaagtag tatatggaat
     3301 ttcacacagg attgtgccta tcagaatagc gataggaatc tcggattaaa atcaggaaag
     3361 tagggttact tttttatgac tacaaatcta ttatgtgatt ccatggcaat ccatcctatt
     3421 gaataccgct acgggcacga tgaaatgaaa gctgtatgga atgaagagac gcggcttttg
     3481 aaaatgttgt ctgttgaaga ggccctggca aaatcccagg tgcgcctgaa ttatatcccg
     3541 aagggaatgg atgaaaagat ttcacttggc gtaaagcttg taagacttaa ccgggtcaag
     3601 gagatagaag atgagatcca tcatgatgta atggctgtgg tccttgccct ggccgagcaa
     3661 tcccaagatg cgggaaaatg gatacatttc ggtgcaactt ccaatgatat tttagatacg
     3721 gcaacagctc ttcagataaa agacgccctc tttatattac gaaaagagac tattaaatta
     3781 cgtcaggttc ttatcgaaag cgctttgtcc cataaaaata ccgtatgtgc agggcgtacg
     3841 cacggacaga taggcgttcc tactacttat ggtctgcgtt ttgcgatatg ggcatcggag
     3901 attgacaggc acattgaaag gcttgatcag cttatgccca gggcaactgt aggtaagatg
     3961 agcggagcag ttggcacgca ggccgcattc ggggaaaaag gcatcgaaat ccagaaaaga
     4021 accatggaat atctcggaat tgaagctgcc gatgtttcaa accagataat ccagcgggac
     4081 aggcatgcag aattcgtgat gtggatggca aatactgtta ctactcttga taagatatgc
     4141 attgaaatcc gaagccttgc cagaagcgag atcgcagaag tagaagaaag ctttggaaaa
     4201 aaacaggtag gctcatctac aatgccgcac aagcgcaatc ccataaaatc agaacaggta
     4261 tgcggacttg cacggatagt ccgggcaatg gtggaacctg agctcaggaa caatacattg
     4321 tgggatgagc gggacctgac aaactcatca tgcgaacgca ttgtattccc ggaatcctgt
     4381 gtgttgaccg accatgtcat acgccttaca acaacaatta tccagaacct tcgcttttat
     4441 cctgaaaata tcaggaaaaa cctggacctt cttaatgggc tgaatatggg agaagccatc
     4501 atgatcgagc tcagtaagaa aggtgtagga cggcaggaag cccatgagat agtacgtcag
     4561 tgtgccatgt ctgcacggga atcgggggta catatgaagg acgctcttct aaagaatgaa
     4621 accgtttcaa aatatatgaa tgaaagtgag ataatcagga taatgaaccc ggataattat
     4681 ataggcacgg cggtaggaca ggtggattcc cttgctgcaa agcttagaaa acggaaataa
     4741 agaggataca taattgttaa gaaaagctac catcaaagat gtggaaaaga tatggaaact
     4801 tgtgaatagt tatgcagata aacgtataat gcttccccgt tcattgagtg aattatatga
     4861 aaatctcagg gatttttatg tagtcatcga agacaataaa cttctgggct gcggggcgct
     4921 tcatattaca tgggaagatt atggggagat actgtctctt gcagttgaac ctgccatggt
     4981 ccggcaaggg atcggttctc agatacttaa agcatgtgaa gaggaggcaa taacacttgg
     5041 attaaataag cttattaccc tcacatatgc gcctgaattt tttgagtcgc atgggtttgt
     5101 cagggtaaaa aagagcacac ttccgcataa gatatggagc atgtgtataa agtgtcccaa
     5161 gttccccgag tgcgatgaga taccgcttgt taaaaagata atctgaggta ttcataatat
     5221 taaaagggct gctggcaaaa gatatagtta aaaattaggt tactcttcaa cataaacagt
     5281 taatataaag aatgacataa tataacaagg ataataaccc actcatgcca tacgctgcaa
     5341 aagacagaca agaattcata gatcgttcaa acttaaaacc tgattcccac attcttgatt
     5401 ttgggggaat gttaattgaa gagcttacaa gtgccgttta tggaataaca tcgaaaagat
     5461 cgctttcaaa tgcagtcctc ataagaccgc tccttttacc atttaagaat tcagtattca
     5521 gatcggtaat ttcttaccat tattttgacc ttgtttcatc tgataaactc ggatttgcct
     5581 ttgaagaggc ctcaagagtg cttgataaag atggcagctt ttcttttatg atactgcagt
     5641 ggaccgcaaa taatgaagcg caaaagtcaa atttactttt caatgaagtc ctgaaaagta
     5701 ttggggttct ttatcagcat gatcttgaag aaataagcat gaatctaaat aaatcgggat
     5761 tcactgagat aactgttgaa agtattaaac gggagatacc cataccgcag gaatttatca
     5821 acgaacactt attgatgctt gggaaccttg taaaaataga gaaaaaaaag gatgggatgg
     5881 aaataaaggc gcttgcaaaa cagtattttc accatataaa agagcacggt gaagccctgc
     5941 tgccggctat tcattttatc gcaaagaaat aatatgaaaa aaatacatct tgagatggtc
     6001 cttgaaaagg tggaaggttt caagtcaccc agtgcatgca aagaacaata tgcgaccccg
     6061 gcaacagttg cgtcggagct attgtatttt gccttcatga ggggagatct cgtggatacg
     6121 gtttatgatc ttggatgcgg aagcggtatc tttgctattg gtgcaaaact cctgggggca
     6181 gaaaaggtta taggttttga tgatgataaa gatgtccttg agattgcacg ggcaaatgca
     6241 aagaaacttg gcgttgaggt tgaattcgta tgctgcaata tttcggacgt tagtggaaaa
     6301 gcgcatacgg ttgtaatgaa tccgcctttt ggcgcgcaga aaaaaggaag tgatcgccct
     6361 tttttgagga aagccctcga attaagcaat gcggtttatt ccatacataa tgccggaagt
     6421 gcagaattca taaaaaaatt cataagccca tcggtcataa cagaatacag gttaatagat
     6481 ttcccgataa aaaggacatt cgggttccat aagaaggaaa gactggtcat aaaagttgaa
     6541 atatacagga tcgagaaaag aatagaggga taattattag aatcaaaaga aaaaaaataa
     6601 caagcaggag aaagcttgga aagggtgacg aaaaagaacc taaagaggta aaagaaagcg
     6661 aactcgagct ggaagaagag atcgaagaag tgactgcgga atcggaaatt gtggaagaga
     6721 atgtagagga aacggttcag ccccaacaac ctgttgaaga agcagcagag gaagaaccaa
     6781 aagaagaaac caggagtgtt ttacctggcg acctta
//
LOCUS       DATGBN010000378         3461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_419054, whole genome shotgun sequence.
ACCESSION   DATGBN010000378 DATGBN010000000
VERSION     DATGBN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419054"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /gene="atwA"
                     /locus_tag="VKL21_09395"
     CDS             complement(<1..1870)
                     /gene="atwA"
                     /locus_tag="VKL21_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HMB46024.1"
                     /translation="MAALIEIRDLSISANGKKILKKINLDINEGDSIGIIGKSGAGKS
                     TLLHLLRGFEEFEDITGEVIFNISSCPKCGRVNPPGNAGKACSKCDIKTELKRVNYLN
                     SKDMHRKIMDRTAIMMQRTFGLYGDETVIENIIRSFESSDIPKEKRPYVAAELIEKVK
                     LSHRMTYTGKELSGGEKQRVVLARQLAKYPMLLLADEPTGTLDPRTAKLVHDSILKAK
                     QEHNMTMLVTSHLPGVLHDLTNKVILLDKGEIIEIGKPDEIIEKFSAMTGIVYEGKAI
                     VGEPIIILKDLKKKYYSYSKGMIPAVNGVSFEVNEGEIFGILGISGAGKTTLSKIIAG
                     IMERDSGKVDVRIGDIWVDMTEKGTDFRGRAKPHIGYLHQEYSLYPHRNVLSNLTDSI
                     GLKFEPELARTKTIAALKAVGFDEKTALDILEKTSYELSVGERQRVTMAQVLIREPRI
                     IIFDEPTGTMDPITKNEVANSILTARKETGTTFVIVSHDMEFVRNVCDRTVHMKLGKI
                     TAMGDAGSVLEEIKIEEKADREKTPEDRNNDLERFLKRAQQCTDLNVLSDVDFYVSKA
                     KETAVKLNKDISGELERLKPAYEKEISEMFKEAERYASEGQIYGMHVYIENAINYAT"
     gene            complement(1872..2210)
                     /locus_tag="VKL21_09400"
     CDS             complement(1872..2210)
                     /locus_tag="VKL21_09400"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMB46025.1"
                     /translation="MLNGDLTTAKKYFFSALSDETRLSILYALKENGDMCVNDICKAT
                     GKDQSLVSHHMSCLRNCGLVNTEKNGKFVVYSIKNELISQILDISDKHVKEMFEGILS
                     CEVVKNKSRS"
     gene            complement(2287..2931)
                     /locus_tag="VKL21_09405"
     CDS             complement(2287..2931)
                     /locus_tag="VKL21_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMB46026.1"
                     /translation="MSSNSITDLSSYWNKWASVWDIMLRLVRLDCRYCEQGISVLNLR
                     KGMTVLDIACGTGLNFPYIFQAVGPEGRIIAVDIAPKMLQKAKERANKNCWNNVEFIQ
                     GDESRIELPDADAAAAFWCMISIPEHQMALENIISSLLSDGRLAVLDFKNINDFPGAV
                     LNPVFNQICRFTHQDVNREPWLYMEQLIENVKMREWKLGGLLLSNAYLAWGEKK"
     gene            complement(2936..>3461)
                     /locus_tag="VKL21_09410"
     CDS             complement(2936..>3461)
                     /locus_tag="VKL21_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46027.1"
                     /translation="SKTLRKDFEVFVDDFKNVLKYSWIFPHRESTSIGMGGPTGKAAL
                     MRRSLDDLCERLSIEKTRFEAARINIDYRGFVFGNRFLAGDAAGIASGLTGEGIYQAI
                     LSGEEVAKGILNKEYTCPRIKHLLKIKTRQERLLHILGMNQYIPHIVFNNAVPFLLKN
                     RGLCRMINDKYNLV"
BASE COUNT          898 a          822 c          566 g         1175 t
ORIGIN      
        1 tagttgcata atttattgca ttttctatat acacatgcat tccatatatt tgtccttctg
       61 atgcatatct ctctgcttct ttaaacattt cagagatttc tttttcatat gctggcttta
      121 gcctttcaag ttcgccgctg atatccttgt tgagttttac agcagtctcc tttgcctttg
      181 atacataaaa atcaacatcg gacaaaacat tcagatctgt gcactgctgt gctcttttaa
      241 gaaatctttc caagtcatta tttctgtctt caggagtctt ttcccggtct gctttttctt
      301 cgatcttaat ttcctcaagc accgaaccag catcgcccat cgctgttatt ttgccaagct
      361 tcatgtgtac agtgcggtca caaacattcc tgacaaattc catgtcgtga gaaactataa
      421 caaatgttgt ccccgtctct tttcttgctg tcaggatcga gtttgcaacc tcattctttg
      481 tgatcgggtc catcgttcct gttggttcgt caaaaataat aattctcggt tctcttatga
      541 gaacctgcgc catggtcacc ctctgacgtt ctccaacact gagttcatat gatgtctttt
      601 ccaggatatc aagtgctgtt ttttcatcaa agcccactgc tttcaatgca gcaatcgttt
      661 ttgttcttgc gagttcaggc tcaaatttta aacctatcga atcggtcaga ttagataata
      721 catttctgtg aggataaagg gaatattcct gatgcaagta accaatatgc ggttttgcgc
      781 gtcccctgaa atctgttcct ttctcggtca tatcaaccca tatatccccg attcttacat
      841 ccacttttcc gctgtctctc tccataatgc ctgctatgat cttagagagt gttgttttcc
      901 ctgctccact tatgcccagt ataccgaaaa tttctccttc gttgacttca aagctcacgc
      961 catttacggc agggatcata ccttttgaat aagagtaata ttttttcttc aaatccttaa
     1021 gaataatgat cggctcaccg acaattgctt tgccctcata cactatcccc gtcattgcag
     1081 agaacttttc aataatttca tccggttttc ctatttctat aatttctcct ttatccagaa
     1141 ggatcacttt atttgtcaga tcatgtaaaa caccaggcag atgcgaagta acaagcatag
     1201 tcatgttgtg ttcctgtttt gcttttaata tgctatcatg caccagcttt gcagttcttg
     1261 gatcaagtgt tccggttggt tcatccgcaa gcaaaagcat cgggtatttc gcaagctgcc
     1321 ttgccagcac tacgcgctgt ttctctcccc cgctgagttc ttttccagtg taagtcattc
     1381 tatggctgag cttcactttt tctattaatt ctgctgcaac atagggacgt ttttctttag
     1441 gaatatcaga agattcaaag ctgcgaatga tattctcaat aactgtctca tcgccatata
     1501 atccaaatgt tcgctgcatc atgatagcag tcctgtccat gattttcctg tgcatgtcct
     1561 tcgaattaag ataattcacc cttttcaatt ctgtttttat gtcacatttt gagcaagctt
     1621 tgcctgcatt acctggagga ttaactctac cgcatttagg acaggatgaa atgttaaaaa
     1681 taacctcgcc agtaatatct tcaaattcct caaaacctcg aagcaaatgt aaaagagtac
     1741 tttttcctgc accgctcttt ccaattatac cgatgctatc gccttcattt atgtccaggt
     1801 ttattttctt gagaatcttt ttgccattag cacttatgct caaatctcgt atctcaatta
     1861 aagctgccat tttaactcct acttttgttt tttacaacct cgcaagagag tattccttca
     1921 aacatttcct tcacatgctt atccgagata tcaagaattt gtgagataag ttcattctta
     1981 atagaataga ccacaaattt tccatttttc tcggtgttga caagaccaca gtttcgtaag
     2041 catgacatgt ggtgtgatac gaggctctgg tcttttccag ttgccttgca gatatcattc
     2101 acgcacatgt caccattctc ttttaatgcg tagagtattg aaagtctggt ttcatcactt
     2161 aaagcactga agaaatactt ttttgctgta gtcaaatctc catttaacat atgtacatat
     2221 gttcatatgt tatcttataa atactttttg ctcaaagatc atttttataa taaaatctaa
     2281 catgtgttac tttttctctc cccatgccag atatgcatta ctcaacaaca gtccaccaag
     2341 tttccattcg cgcatcttta cattttcgat cagttgctcc atatacaacc atggttcacg
     2401 gttcacatcc tgatgagtaa atcggcagat ttgattgaag accggattaa gtaccgcacc
     2461 aggaaaatcg ttaatattct taaaatcaag caccgcgagc ctgccatcag ataaaagact
     2521 tgaaatgata ttttccagag ccatttgatg ctcagggata cttatcatac accagaacgc
     2581 agccgcagca tctgcatctg gaagttcgat ccggctctca tcaccctgga tgaactcaac
     2641 attattccag cagttcttat ttgctcgttc ttttgccttt tgaagcattt taggcgcaat
     2701 atctaccgct ataatccgac cttccggacc gactgcctga aaaatatatg gaaaattaag
     2761 acctgttccg caggcaatat ccaacactgt cattcctttt cttagattta gtaccgagat
     2821 tccttgctcg cagtatctac aatcgagacg aacaaggcgc agcattatat cccacactga
     2881 tgcccatttg ttccaatagg aacttagatc tgttatagaa ttgctgctca tttttttaca
     2941 ccagattgta tttatcgttt atcattctgc ataaccccct attcttcaaa agaaaaggca
     3001 ctgcattatt aaaaacaata tgaggtatat actggttcat acccaagatg tgcagcaggc
     3061 gttcttgacg ggtttttatt tttaataggt gtttaatcct gggacaggta tattctttgt
     3121 tcaggattcc ttttgcaacc tcctctccgg atagaattgc ctggtatatt ccctctccgg
     3181 tcagtcccga cgcaatacca gccgcatcgc cggctaagaa cctgttcccg aaaacaaatc
     3241 ccctataatc aatgttaata cgtgcagctt cgaaccttgt tttttcaatg gagagcctct
     3301 cgcacagatc atcaaggctg cgcctcatca atgcagcttt tccagtcggc ccacccatcc
     3361 ctatcgaggt agattccctg tgtggaaata tccagctata cttaagaaca tttttaaagt
     3421 cgtctacaaa aacctcaaag tcctttctca gtgttttgct t
//
LOCUS       DATGBN010000379         6823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_380560, whole genome shotgun sequence.
ACCESSION   DATGBN010000379 DATGBN010000000
VERSION     DATGBN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380560"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VKL21_09415"
     CDS             <1..291
                     /locus_tag="VKL21_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46028.1"
                     /translation="IRMQRDLYSAFLAKCVGKDTSALEFARAKQLWQGMESILSDAIS
                     RAKQTAIGKVCPASFGFTRIQSQSGSLVNPIRIISEAMDAVILPDESHRELV"
     gene            352..480
                     /locus_tag="VKL21_09420"
     CDS             352..480
                     /locus_tag="VKL21_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46029.1"
                     /translation="MAKFELLLLKDRQKRGIAAASIRGKHLGRPTKKMRIAQHGTT"
     gene            complement(754..828)
                     /locus_tag="VKL21_09425"
     tRNA            complement(754..828)
                     /locus_tag="VKL21_09425"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(790..792),aa:Glu,seq:ctc)
     gene            1001..1417
                     /locus_tag="VKL21_09430"
     CDS             1001..1417
                     /locus_tag="VKL21_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF22 domain-containing protein"
                     /protein_id="HMB46030.1"
                     /translation="MSEIVNVVSRSNGKVSRKKVKASPYEFTIATRAKWEMVIADEDI
                     TIGAGKLERVKVKEITVQKDMLAIPCAFSHHPIVSVVKVATKEGPSPVEIDRTINVAY
                     VIGQESGEINKGDLLSVLNLYPIMFTREATKPVCIG"
     gene            1423..1923
                     /locus_tag="VKL21_09435"
     CDS             1423..1923
                     /locus_tag="VKL21_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46031.1"
                     /translation="METCKICKGDQDSKHYKGYNICTECADLMEDLMGEYFLRTISDS
                     VETKKGYLKYLENGKEFISNYQKIRQHSKRHIKHMEEHVQEEMKGAQGGKQRYLERLL
                     QTINWLEHCPEFYNYYFREYYVCPSCGASIFDHYDKQEIGDWLMITCEKCDTVIKKYF
                     SPKFVS"
     gene            2014..2181
                     /locus_tag="VKL21_09440"
     CDS             2014..2181
                     /locus_tag="VKL21_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46032.1"
                     /translation="MCSTYTGFSMMPADLEEMKKGSSMFWCVSCKKTFLKKGATECPN
                     CSSKNIKARAL"
     gene            complement(2240..4387)
                     /locus_tag="VKL21_09445"
     CDS             complement(2240..4387)
                     /locus_tag="VKL21_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HMB46033.1"
                     /translation="MFTKLIFPPQWIPTQPYLSLPSLTAFLKANKCDVEQIDINVSFY
                     DHVLSKKGLQVYYDRAQIKFQGLESAKELSPELQKQYAALSSSILFGNRVINGVDDAK
                     KITQSKEDFFDIEKLFNAFKILELGLKLASSAYYPSNLTFHSYDMRYSCRSSKAILEA
                     INDRNENIFIEYFEKYTLPEVLKGNPGLVGISIINISQLIPGLTLANIIKKADPDIHI
                     NIGGSVFTRLVNEISHNKELFAVFDSVIVHEGETPLLNLIRHLNNEFDIRDVPNLIYK
                     EGPEIIVNNLRSNGEDINSLPTPCFGGLPLDKYLSPELVIPVLSSRGCYWNRCTFCDH
                     GFGYSGRYRPRNVDLLFNDIKTLNDKHKTNFFTFQDEGLSTKIIGALSDRIIEAKMDI
                     SWLADSRFEASFSQELGTKLSLSGCKMLYFGLESANDRVLKCMDKGIKKENVLKICKY
                     CNDTGIWAHLFLIFGFPTETHDEARETMEFILHNNDIIRSMSFGSFQLTKHSKVYENP
                     SMFNVSKIYQDEEIDLSLWYDCDITEGLSKKDADGVIQEFYNKLSNRYLDLPIWSNLD
                     REHLFLYIAHYKKSINKVADLSELIRAIRGRKKSGNKSIEKRNFKPVLNEGIFIGTFN
                     FNLARIIHSSLTGNIPGNIQKEKVNVLFDIDNNRIFTISDLARDILEKSNGESNISEI
                     TDLIKEKYKLPYNEAESKCKSFLNGLIEKNIVI"
     gene            4524..4832
                     /locus_tag="VKL21_09450"
     CDS             4524..4832
                     /locus_tag="VKL21_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46034.1"
                     /translation="MNDNDTKHIGDEFVEEDENSKSEHEKALEGLYDLVLPPGIPHKL
                     ILEVMDTFNLEVATRKCSVKTVEVEEDNLLTLRGELDSVNQAHDYIFQKLSERYNYKK
                     "
     gene            complement(4903..5160)
                     /locus_tag="VKL21_09455"
     CDS             complement(4903..5160)
                     /locus_tag="VKL21_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46035.1"
                     /translation="MSEAKGEVKRTPKVPGLVKCAYCDGTGKQKDIKCIVCGGKGEVE
                     VVDSSRKCQWCKGRGYMMHGIPCTNCGGTGATRPIGKERLF"
     gene            complement(5268..>6823)
                     /gene="mcrA"
                     /locus_tag="VKL21_09460"
     CDS             complement(5268..>6823)
                     /gene="mcrA"
                     /locus_tag="VKL21_09460"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     alpha"
                     /protein_id="HMB46036.1"
                     /translation="VEMAKCGAAITKKRGLQAYDPKLHLAGIPMGQRQLTPYTISGTD
                     IVCDGDDLHFVNNAAMQQEWDDIRRTCVVGLDLAHETLEKRLGKEITPETINYYLEVL
                     NHAMPGAAIVQEHMVETHPALVDDCYVKIFTGDQTLQDEVDKQFVINIDNEFPDNQAK
                     QIKAAMGKTSWQAVHIPTIVTRTEDGPGTSRWMAMQVGMTFISAYHMCAGEAAVGELA
                     FTAKHAGLVEMGDMIPARRARGPNEPGGLSFGHMADIVQTNRKSPEDPVNVVLQTASA
                     ATMLYDQIWLGGYMSGGVGFTMYATPAYTNDIVDDFLYWGNDYAAKKYGGNGKAKATI
                     DTVKDIATETTLYGLEAYEKYPTTLEDHFGGSQRATVISIAAGGATALATGHSQAGLS
                     AWYLSMYLHKEAHGRLGFYGYDLQDQCGATNVFSIASDEGCIGECRGANYPNYAMNVG
                     HQGGYTSVVAATHAGKDAFCVNPLVKTCFADDLINFDFADPRAAFGKAALREWDRCAG
                     ERAFVIPAK"
BASE COUNT         1995 a         1355 c         1412 g         2061 t
ORIGIN      
        1 atcaggatgc agagagactt gtactctgca tttctggcaa aatgtgttgg aaaagataca
       61 agcgcccttg aatttgccag agcgaaacaa ctctggcaag gtatggaatc tatccttagc
      121 gatgcgattt caagggcgaa acaaactgcg ataggcaagg tctgtcctgc cagctttgga
      181 ttcacgagaa tccagagcca gagtggttcg ctcgttaatc ctatacgaat tataagtgag
      241 gctatggatg ctgtaatcct cccggatgag agccatagag agcttgtatg aacgtgagga
      301 atctcccggc tttagctgtg ggagaggttc aactggtggc tgcagatagg gatggcaaag
      361 tttgaattgt tgttgctcaa ggaccgtcag aaaagaggga ttgctgcagc aagtataaga
      421 ggaaaacatc ttggaaggcc tacaaaaaaa atgaggatcg cgcaacatgg cacgacctaa
      481 taatcatact acaaaacgta tgcagaggat agacttcaca gacactatta ctccgtatgt
      541 gtatcatcat aaagtgttat ggttcaaccc aattacgaat catatcacat attttgctcg
      601 atttcgcata aacaaaagat agtcggaggg gggatgaccc taaattctga gaggctgaaa
      661 ataaatggtg gtgttgagaa ggaacatccc ctcctcaaac aatatgtgaa catcattata
      721 tcgactgttg tgagtgaata ataatgagac agatgctccg atggggattt gaaccccagt
      781 cgtcagagtg agagtctgac atgattggcc gtgctacact atcggagcgg gattacctca
      841 aagtaattat gtattaatta aagtttcgtt tgcagccagg gaaattttta gagagttgat
      901 atttatcaca cctttattaa atatcatgat taataagtat aatgattaac cgtgataaac
      961 tataagtact ttgatattca tacaaaagga aggtgaaacc atgagtgaaa tagtaaatgt
     1021 ggtttcccgt agcaacggga aagtatcaag aaaaaaagtc aaagcatcgc cgtatgaatt
     1081 tacaatagct accagggcaa agtgggaaat ggtaattgca gatgaggaca tcacaatagg
     1141 ggccggtaag cttgagcgtg tcaaagtaaa ggaaattacg gtgcagaaag acatgcttgc
     1201 cataccctgc gctttcagcc accatccaat tgtttctgtc gtaaaggtgg caaccaaaga
     1261 aggtccttct cctgtagaaa ttgaccggac catcaacgta gcttatgtaa tcggacagga
     1321 atcaggtgag atcaataaag gagacctgct gtcagtattg aacctttatc ccataatgtt
     1381 cacacgagaa gcgactaaac cggtgtgtat cgggtgataa taatggaaac ctgcaagata
     1441 tgcaagggag accaggattc aaaacattat aagggctaca atatttgtac ggaatgcgca
     1501 gacctcatgg aagacctgat gggcgaatat tttctgagaa ccataagtga cagtgtggaa
     1561 acaaaaaaag gatacctgaa atacctggaa aatggaaaag aattcataag caattatcaa
     1621 aaaattcggc agcacagtaa aagacatata aagcatatgg aagaacacgt gcaggaggag
     1681 atgaaaggtg cacagggcgg aaagcagaga taccttgaaa ggcttctcca gaccataaac
     1741 tggcttgaac attgtcctga gttctacaat tattatttca gggaatacta tgtatgcccc
     1801 agttgcggag catccatatt cgaccactat gataaacagg aaattggaga ttggctcatg
     1861 ataacatgcg aaaaatgcga tacagtgatc aagaagtatt tttcgccaaa attcgtgagc
     1921 tgattttttt ttccaaatcg caaggtttta atatcgaaag ggacaaacaa taaaatgcaa
     1981 taaaataaga tttgattaat ggaggttgga atcatgtgca gtacatatac gggattttca
     2041 atgatgccgg cagaccttga agagatgaaa aaaggaagct cgatgttctg gtgtgtttca
     2101 tgtaagaaga catttctaaa gaaaggggca acagaatgcc cgaactgcag ctcaaagaat
     2161 atcaaggcaa gagcactgtg aactacccct tccttttaga acggtacttc tgggcccctt
     2221 gaccacgcaa ggtgaaattt catattacaa tattcttttc tatcaaacca ttcaaaaagc
     2281 ttttgcattt cgattcagct tcgttataag ggagtttata tttttcttta attagatctg
     2341 ttatttcaga aatatttgat tccccattac tcttctccag tatatccctt gcaagatcag
     2401 aaattgtaaa tatcctgtta ttatctatat caaatagaac attcactttt tctttttgaa
     2461 tatttccagg aatatttccc gtaaggctgg aatggattat cctggccagg ttgaaattga
     2521 aagtcccgat aaagatccct tcattcagga ctggtttgaa attcctcttt tcaatcgatt
     2581 tattcccgga cttcttcctg cctcgtatag cccggatcaa ttcagataag tctgctacct
     2641 tgtttatgga tttcttgtaa tgggcaatat acaggaaaag atgctccctg tcaagattgc
     2701 tccatatagg caaatccaaa tatctatttg aaagcttatt atagaattcc tgaattacac
     2761 catcggcatc tttttttgat aatccttctg ttatatcaca atcataccat aaagaaaggt
     2821 ctatttcttc atcctggtag attttagaga cattgaacat tgaaggattt tcatatactt
     2881 ttgaatgttt ggtaagctgg aaactcccaa acgacatgga acggattatg tcattattat
     2941 gcaaaataaa ttccatggtt tctcttgctt cgtcatgagt ttcggtagga aacccgaata
     3001 tcaaaaacag gtgtgcccag attccggtat cattgcaata tttgcagatt ttcaatacat
     3061 tctctttctt tatcccttta tccatgcact taaggaccct atcgttagct gattcaagcc
     3121 cgaaataaag catcttacag ccggagaggg agagttttgt gcctagctcc tgtgaaaatg
     3181 acgcctcaaa tcttgaatct gccaaccatg aaatatccat ttttgcctcg attatcctat
     3241 cagataatgc gccgattatt ttagtggata gaccctcatc ctggaaagtg aaaaaattgg
     3301 ttttgtgttt atcattcaaa gtcttgatat cgttaaaaag aaggtcaaca ttccttggcc
     3361 tgtatctacc ggaataacca aacccatgat cacaaaaagt gcagcgattc cagtaacatc
     3421 cccttgatga aagaacagga attacgagct caggcgacaa atacttatca agtgggagac
     3481 caccaaagca tggtgtgggt aaagaattaa tatcttctcc atttgatctt aaattattta
     3541 ctattatttc tgggccttcc ttataaatta aattgggaac atcccggatg tcaaattcat
     3601 tattcaaatg tctaataaga ttcaataagg gagtctcacc ttcatgcact atcacgctgt
     3661 caaaaacagc aaaaagctct ttgttatggc ttatttcatt taccaaccgc gtgaaaacac
     3721 tgccacctat attaatatgt atatccgggt cggctttctt aataatattt gcaagtgtta
     3781 atcctggtat caactggctt atattaatta ttgaaattcc gaccaatccc ggattgcctt
     3841 ttagtacttc tggaagtgtg tatttttcaa aatattcaat aaaaatattt tcgtttcgat
     3901 cattaatagc ttcaagaatt gctttgcttg aacggcatga ataacgcata tcataagaat
     3961 gaaatgtcag gttggaagga taataagcgg aagatgctaa tttgagacca agctccagta
     4021 tcttaaatgc attgaatagt ttttctatat cgaaaaaatc ttctttgctc tgtgtgattt
     4081 tttttgcatc atcaacacca ttaatgacgc gatttccaaa aagtatggaa ctactcaaag
     4141 ctgcatattg tttttgaagt tcaggagata attcttttgc ggattcaagt ccctggaatt
     4201 ttatttgcgc cctatcatag taaacctgaa gccctttttt gcttaaaaca tgatcataaa
     4261 aggaaacatt gatatcaatt tgttccacat cacatttatt cgctttcaaa aatgctgtta
     4321 gagaaggaag actgagatat ggctgagttg gaatccattg cggcggaaag atcaattttg
     4381 tgaacataat ttttgatgaa tatggatatt aaggataaaa cggtttcgac attttcatgt
     4441 tttttttgaa tattaatatt gttcaatatt gctcaccaca aactttattt gacttgataa
     4501 tgatttgatg taaagtgatc cccatgaatg ataatgatac aaaacatatc ggcgatgaat
     4561 ttgttgaaga agatgagaac tcaaaaagtg aacatgagaa agcattagaa ggattatatg
     4621 atctggtttt gcctccggga attcctcata aactgatcct tgaagtaatg gatacattca
     4681 atctggaagt tgcgacccgg aagtgttcag taaagacagt agaagtagag gaagataatc
     4741 ttcttacatt acgaggagag cttgattctg taaatcaggc gcatgattat atttttcaaa
     4801 aattgagtga aagatataat tacaagaaat aattcttttt ttcaattctt taataacaga
     4861 ttttgataaa gatatagggg aaacccctat ttctctataa tattaaaaca ggcgttcttt
     4921 gccaataggt cttgtagcgc cagttccacc acaattagtg catggtatgc catgcatcat
     4981 ataacctctt cccttgcacc actggcattt tcgggatgag tctactactt ctacttctcc
     5041 ttttccgccg catacaatac attttatgtc cttttgtttt ccagtcccgt cacaatacgc
     5101 gcatttcact aagccgggta cttttggcgt tctttttact tctccttttg cttctgacat
     5161 tttaaatcac atcaccataa atctcaaact aagttataaa tatttccttt taatttttgt
     5221 ttctcaaaaa aatagagaaa gtaggcattt agcctactta acaatgttta ctttgctggg
     5281 ataacgaagg ctctctcacc ggcacagcgg tcccactctc tgagtgctgc cttgccgaat
     5341 gctgcccttg gatctgcgaa gtcaaagttg atcaagtcat ctgcgaagca ggtcttgact
     5401 aacggattga cgcagaacgc atccttgcca gcgtgagttg ctgctactac tgaggtgtat
     5461 ccaccctggt gacctacgtt cattgcgtag ttggggtagt ttgcgcctct gcattcgcct
     5521 atgcagcctt catctgatgc aattgagaac acgtttgttg caccgcactg atcctgcagg
     5581 tcatacccgt agaatccaag tctgccgtgt gcctctttgt ggaggtacat ggacagatac
     5641 catgcagaga gaccggcctg gctgtggcct gttgccagag ctgttgcgcc gcctgctgca
     5701 attgatatga cagttgctct ctgtgagccg ccaaagtggt cttccagtgt tgttgggtac
     5761 ttctcgtatg cttcaagacc gtacagtgtt gtttctgtag cgatatcttt tacagtgtcg
     5821 atggtggctt ttgcctttcc atttccgccg tatttctttg ctgcatagtc gttgccccag
     5881 tacaggaagt catcgacgat atcattggtg tatgctggtg ttgcatacat tgtgaaaccg
     5941 acgccgcctg acatgtagcc accgagccag atctggtcat acagcattgt ggctgcgctt
     6001 gcagtctgca atactacatt gactggatct tctggactct ttctgtttgt ctggacgata
     6061 tccgccatat gaccgaagga taatcctcct ggttcatttg gacctcttgc tctgcgtgct
     6121 gggatcatat cacccatttc gacaagtcct gcgtgtttgg ctgtgaatgc aagttctccg
     6181 actgctgctt caccggcgca catatggtat gcgctgataa aggtcatacc gacctgcatg
     6241 gccatccagc ggcttgttcc tggtccatct tcagttcttg tgacgatggt tgggatgtgt
     6301 actgcctgcc aggaggtctt gcccattgcg gccttgatct gttttgcctg gttgtcaggg
     6361 aattcgttgt caatgttgat aacgaactgt ttatctactt catcctgtaa agtttgatca
     6421 ccagtgaaaa tcttcacata gcagtcatct actaatgctg ggtgtgtttc aaccatgtgt
     6481 tcctggacga ttgctgcgcc aggcattgcg tggttcaaaa cttcaagata gtagttgatt
     6541 gtttccgggg tgatctcttt gcccaatctc ttctcaagag tctcatgtgc aaggtcaaga
     6601 cctacgacgc atgttctcct gatatcgtcc cattcctgct gcattgctgc attgttgacg
     6661 aagtgaagat cgtcaccatc gcatactata tctgttccag atattgtgta tggggtcaac
     6721 tgtctctgac ccatggggat acctgctaag tgcaacttgg gatcataagc ctgcaatcct
     6781 ctcttcttgg tgattgctgc tccgcacttt gccatttcta cct
//
LOCUS       DATGBN010000380         6575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_302555, whole genome shotgun sequence.
ACCESSION   DATGBN010000380 DATGBN010000000
VERSION     DATGBN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302555"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..3021
                     /locus_tag="VKL21_09465"
     CDS             388..3021
                     /locus_tag="VKL21_09465"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HMB46037.1"
                     /translation="MVELFVRESAIATEKAKNENSAPEMQKTGEVKNIEKPKNRVHEK
                     VVYSHEEAVEASILYFKGDELAARVWANKYALKDSFGNLYEKSPDDMHHRLAREIHRV
                     EMKYKNPLSEELIYSVLKDFRYIVPQGGPMTGIGNDYQIASLSNCFVIGNDGSSDSYG
                     GIMKIDQEQVQLMKRRGGVGHDLSHIRPKGTPVLNSALTSTGVVPFMERYSNSTREVA
                     QDGRRGALMLSISIKHPDSGKFIDAKLESGKVTGANVSVKLTDDFMKAVETKTLFRQQ
                     YPINAENPKFAKDIDAVQLWGKIIHNAWKSAEPGVLFWDTIIRESVPDCYSDLGYRTV
                     STNPCGEIPLCPYDSCRLLAINLFSYVNTPFTGKAEFDFDLYKEHVGLAQRMMDDIID
                     LELEKIDVILTKIHADPEIEEIKLVEENLWKNIRMKAEEGRRTGIGITAEGDMLAGLG
                     LRYGSDEATDFSVEVHKTLALEAYKASTYLAKERGAFTIYDAEREKENPFILRMKEAD
                     PVMYNNMVKYGRRNIALLTIAPTGTTSLMTQTTSGIEPIFATFYKRRRKVNPNDLDVK
                     VAFKDEVGDSWEEFNVFHHKFIDWLKLNGYDSDEVTRMGDEEIESIIAKSPYYKATAN
                     DVDWIAKVKMQGAIQKWVDHSISVTINLPAEAKEELVSELYLTAWKSGCKGATVYRDG
                     SRNGVLIAGKTEVRPEKPKRPKSLDCDVIRFNINEEKWIAFVGLKESRPYEIFTGIGD
                     EEIFPIPKSIVKGKIIKINDEDGKTRYDFQYTDKYGYKKNIEGLSHMFKPEFWNYAKL
                     ISGVLRHEMPIQDVVNLVTSLKLDSESINNWKNGVERALKKYIPNGTKAKGKCGECGS
                     DNLVYEEGCLICKDCGSSKCG"
     gene            complement(3029..4219)
                     /gene="kbl"
                     /locus_tag="VKL21_09470"
     CDS             complement(3029..4219)
                     /gene="kbl"
                     /locus_tag="VKL21_09470"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HMB46038.1"
                     /translation="MYNNYQNHLVKIIEEINEAGLYKNERIIVSPQGAEIELNSGQKV
                     LNFCANNYLGLSNNSSLIEAAKASLDDHGYGMSSVRFICGTTDLHKKLEKKIAEFFGT
                     EDTVLYAACFDANGGVFEPLLTEEDAIISDSLNHASIIDGVRLCKAQRYRYENADMTD
                     LEKQLQLAQNQRFRLIVTDGVFSMDGNVAPLDKIVDLANKYNAMVMVDESHSAGVVGK
                     TGRGVTELYNIKGKVEIITGTLGKAFGGAIGGFTTGKKEIIEILRQRSRPYLFSNSIP
                     PMIAAAGIRMIEMMTETNVLQDKLHFNAEYFITSMKKLGFDIKPTKSAICAVMLYDAR
                     LSQDFAARLLKEGIYVIGFYFPVVPKGQARIRVQLSAGHEREHLDKAIAAFKKIGKEL
                     AVIK"
     gene            complement(4237..5271)
                     /gene="tdh"
                     /locus_tag="VKL21_09475"
     CDS             complement(4237..5271)
                     /gene="tdh"
                     /locus_tag="VKL21_09475"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HMB46039.1"
                     /translation="MNKMKALVKARPEKGLWMEKVPIPEPGLNDVIIKVKKSAICGTD
                     LHIYEWDAWSQKTIRTPMIIGHEYMGYIEKMGAGVTNLKIGERVTGEGHLACGHCRNC
                     RRGKEHVCENTVGIGVHIDGSFAEYVKVPAKNVIPLDHRIPDEWAAIMDPFGNATHTA
                     LSFPMIGEDVLITGSGLIGSMAVAIAKFAGARFIVASDLSDSRLEIAKKMGATLTVNP
                     SKGEKISDALKTLGMRGFDIGLEMSGSPKAFLDMLKNMYNGSSISLLGILPSNFEVPW
                     SDIIFKAITLKGIYGREMWETWYKMEQMIIGGIDLNPVITHRFSIDDFQKGFDEMEKG
                     NCGKVILSWY"
     gene            5387..>6575
                     /gene="gltX"
                     /locus_tag="VKL21_09480"
     CDS             5387..>6575
                     /gene="gltX"
                     /locus_tag="VKL21_09480"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMB46040.1"
                     /translation="MKEVRVRFAPSPTGPLHIGGVRTALYNYLFAKKNSGTFILRIED
                     TDQARFVEGAEEYIMESLEWCGIIVDEGIREGGNYGPYRQSDRKTIYRQYADNLIDKG
                     DAYYAFDTPEKLETLRRESEKAGKTFIYNAEVREKLINSVSLKEPEWREKLDKGEPYV
                     IRYKMPYNEDIRFDDIIRGHIVVNTGTLDDKVLFKSDGMPTYHLANIVDDHLMKISHV
                     IRGEEWLPSLPLHILLYRSFGWDPPLFAHLPLLLKPDGKGKLSKRDGDKMGFPVFPLF
                     WPYGETSKGYREDGYYPDAFINMLALLGWNPGTEQELFSMDELINSFSIDRVGKSGSR
                     FDPEKAKWFNHQYLQTKTNNQIAIEFREFLRARGYHIDILRLESLVGMVKERVSFVKD
                     IWEQ"
BASE COUNT         2021 a         1295 c         1408 g         1851 t
ORIGIN      
        1 catggggttg cctgcttggc atacgtctga ttttttagtg aaatatcttt ttaattttta
       61 taaagaggct gtctcaaaaa gacagcctct tttttttcaa attttaggag aggaatattg
      121 taatcggaat ggaacagcgg aaccggcccc tgaatgtggg tgcatgaaca tgaccacata
      181 ttgtagttaa ttatttaaca caataaaatt gcgcaaatag ctgaatatca attattaatg
      241 taatagttca taactatatt gccaaatgtt aataaagtta aaattttact cttgcataga
      301 gaagagtgct tgcttaattt agcaaatacc cgctttaaaa atcagtggcc gtttaatctc
      361 attaaaatca gagagtaaca aaatattatg gtagaattat ttgtccggga atcagctatt
      421 gcaactgaaa aagcaaaaaa cgaaaattca gcgcctgaga tgcagaagac aggtgaagtg
      481 aaaaatattg aaaagccaaa gaatcgagtg catgaaaaag ttgtttattc tcatgaggaa
      541 gcagttgaag caagtattct gtatttcaaa ggcgatgaac ttgctgcgcg ggtatgggca
      601 aacaaatatg ctttaaagga ttctttcggt aacctgtatg aaaagtcacc cgacgacatg
      661 catcacagac tggcccgtga aatacaccgg gtagagatga agtataagaa cccgctttcc
      721 gaagagttga tttatagtgt actgaaagat ttcagatata ttgttccaca gggcggccca
      781 atgacaggca tagggaatga ttatcagatc gcttcattat cgaactgctt tgtgattggt
      841 aacgatggat catccgactc atacggaggt atcatgaaaa tcgatcagga gcaggtacag
      901 cttatgaaac ggaggggcgg agtaggtcac gacctctctc atatccgacc caaaggaact
      961 cctgtgctta acagcgccct cacctcaacc ggtgtggttc ctttcatgga aaggtattct
     1021 aactcgacac gcgaggtagc ccaggatggc aggagaggtg cacttatgct ttcaatctca
     1081 atcaaacacc ccgactcagg gaaattcatt gatgctaaac tcgagagcgg taaagttaca
     1141 ggcgctaacg tctcagttaa actgaccgac gatttcatga aagcggttga aaccaaaacg
     1201 ctcttcagac agcaatatcc aattaatgcc gagaatccga aatttgcgaa ggatatagat
     1261 gcagttcagc tctggggtaa aattatccat aacgcctgga agtcagctga gccaggggta
     1321 ttgttctggg atacaataat cagagagagt gttcccgatt gttatagcga tctgggatat
     1381 agaacagttt cgacaaatcc gtgtggtgaa attccacttt gcccctacga cagctgtcgt
     1441 ctgcttgcaa taaacctgtt cagctatgtt aacactccat ttaccgggaa ggctgaattt
     1501 gactttgatt tgtataaaga gcatgtagga ctggcacaac ggatgatgga tgacattatt
     1561 gatcttgaac ttgaaaaaat cgatgtaatt cttacaaaaa tacatgcaga cccggagatt
     1621 gaagagataa aacttgttga ggaaaacctc tggaaaaaca tcaggatgaa agcggaagag
     1681 ggacgaagaa ccggaattgg aataacagca gaaggagata tgctcgcggg tcttggtctg
     1741 agatatggca gcgatgaagc tacagacttt tcggtcgaag ttcacaaaac acttgcactc
     1801 gaagcttata aagcatcgac ataccttgca aaagaaagag gagcctttac aatatatgat
     1861 gcagaacgcg aaaaggagaa cccttttatt ctgaggatga aagaggctga ccctgttatg
     1921 tacaataaca tggttaaata tggacgcaga aacatagctc tgcttacaat tgcccctacc
     1981 ggcactacaa gtcttatgac ccagacaact tccggaattg aaccgatatt tgcaactttc
     2041 tataaaagaa gaaggaaagt taaccctaat gatctggatg taaaggtggc atttaaagat
     2101 gaggttggcg attcgtggga agagtttaat gttttccatc ataaatttat cgactggctc
     2161 aagcttaatg gatacgattc cgatgaggtt acaaggatgg gagatgagga gattgaatcg
     2221 attatcgcaa aatcacccta ctataaagcc acggcaaatg atgttgactg gatcgcaaaa
     2281 gttaagatgc agggtgcaat tcaaaaatgg gtggaccatt caataagtgt tacaataaac
     2341 cttcctgctg aagcaaaaga agaactggtc agcgaactct atctgacagc atggaaatca
     2401 ggatgcaaag gcgcaactgt ttatcgcgac ggatccagaa acggggtact gatagcagga
     2461 aaaacagaag taaggcccga aaagccaaaa cgtccaaaat cgcttgattg cgatgttatc
     2521 aggtttaata taaatgaaga gaaatggatc gcttttgtag gtctgaaaga gagccgacct
     2581 tatgagatct tcaccggtat aggcgacgag gagatattcc ctattccaaa aagcatagtt
     2641 aaaggtaaga tcataaaaat caatgatgag gatggaaaga cacgttatga ttttcaatat
     2701 accgacaaat acgggtataa aaagaatatt gaaggtcttt cacacatgtt caagcctgag
     2761 ttctggaatt atgcaaagct gatttcaggt gttctcagac acgaaatgcc tatacaggat
     2821 gtagttaatc tggtcacgtc attaaaactt gacagcgagt cgattaataa ctggaagaat
     2881 ggagttgaaa gagccttgaa gaaatatata ccaaacggaa caaaagcaaa gggcaaatgc
     2941 ggggaatgtg gttcagataa ccttgtttac gaagaaggtt gcctgatctg caaagactgc
     3001 ggatcgtcca aatgcggatg atatgatttt attttattac tgcaagttcc ttcccgatct
     3061 ttttaaaagc cgcgatcgct ttatcgagat gttctcgctc gtgacccgcg gaaagctgta
     3121 ctcttattct tgcctgacct ttaggaacga caggaaaata gaatccgata acataaatac
     3181 cttctttaag aagtctggca gcaaagtcct gggataatct ggcatcataa agcataacag
     3241 cacagattgc cgatttggta ggctttatgt cgaaaccaag ttttttcata ctggtaatga
     3301 agtattcagc attgaaatga agtttgtcct gaagcacatt tgtttcagtc atcatctcaa
     3361 tcatcctgat acccgctgca gcaatcatag gagggattga gtttgaaaag agatagggcc
     3421 ttgacctttg tctcaggatc tcaattatct cttttttacc tgttgtaaag ccgccaattg
     3481 ccccaccaaa ggctttcccc agtgtccctg taatgatttc aactttacct ttaatattat
     3541 aaagctcagt cacgcctctt cctgtctttc ccactactcc cgcggagtga gattcatcaa
     3601 ccattaccat agcattatat ttgtttgcca gatctacaat tttgtcaagc ggcgctacat
     3661 ttccgtccat tgagaacact ccatcggtga ctataagtct gaatcgctga ttctgagcaa
     3721 gttgaagttg cttttcaaga tctgtcatgt ccgcattttc atatctgtaa cgctgggctt
     3781 tgcaaaggcg tacaccgtcg atgatcgaag catggttcag ggaatcggaa attattgcat
     3841 cctcttcagt gagaaggggt tcaaagactc cgccatttgc gtcaaagcaa gctgcataaa
     3901 gaacagtatc ttcagtgccg aaaaattctg caattttttt ctccagtttt ttatggagat
     3961 cggtggtacc acaaataaac cttactgagg acattccata accatggtca tccaaggatg
     4021 cttttgctgc ttcaataagt gacgaattat ttgaaagccc caggtaatta ttggcacaga
     4081 aattcagtac cttctgccct gaattaagtt caatctctgc gccctgagga gatactataa
     4141 tccgctcgtt cttgtataag ccggcttcat taatctcttc aataatttta actaaatgat
     4201 tctgataatt attatacatg gtcttaatat tattagttaa taccagctta agataacctt
     4261 tccacagttc cctttttcca tctcatcgaa tcctttctgg aaatcatcta ttgagaacct
     4321 gtgggtaata acaggattta aatctattcc tccgataatc atctgttcca tcttgtacca
     4381 tgtttcccac atctctcttc catagatgcc ctttagtgtt attgctttga aaatgatatc
     4441 actccagggt acctcgaagt ttgatggcag tatgccaagc aatgagatac ttgaaccatt
     4501 atacatattt ttgagcatat ctaagaacgc tttgggagat ccggacatct caagacctat
     4561 atcgaatcct ctcataccaa gcgttttaag ggcatccgag attttttctc ctttcgaagg
     4621 gtttacagtc agggttgcgc ccatcttttt cgcaatctca agacgggaat cgctgaggtc
     4681 gctggcaaca atgaatcttg ccccggcaaa ctttgctatt gcaacagcca tactgccaat
     4741 aagacctgat ccggtaatca gtacatcttc accaatcatg gggaaagata acgctgtatg
     4801 agtagcattt ccgaatggat ccataattgc agcccactca tcaggaatcc tgtgatcgag
     4861 gggaatcacg ttttttgcag gaaccttcac gtattctgca aatgatccgt cgatatggac
     4921 tccgattccg acggtgtttt cacagacatg ctctttgccc cggcgacagt tgcggcaatg
     4981 accacatgcc agatggccct ccccggtaac tctttcacct attttgagat tggtcactcc
     5041 tgcacccatt ttttcgatat atcccatata ctcatgacca atgatcattg gggtccggat
     5101 tgttttttgt gaccatgcat cccattcata aatatgaaga tcggtaccac aaatagcaga
     5161 ttttttgacc tttataatga catcgttaag acctggctcc ggtatcggaa ctttctccat
     5221 ccataatcct ttttcaggtc tggctttaac aagtgctttc atcttattca taacgaggta
     5281 attttattgg cgctaaatta atacaaatcc ggagttcaaa acctgatttt cgttatgtaa
     5341 tcgggtttgt ctatatttgc attcacgtta aataatttac cataatatga aggaagtacg
     5401 agtgcgcttt gccccaagtc ctacaggtcc attgcatatc ggcggtgtaa gaaccgctct
     5461 ttacaactat ctttttgcaa agaagaatag cgggactttt atattaagga tcgaggatac
     5521 agatcaggca agattcgttg agggtgccga agaatatatt atggaatcac ttgaatggtg
     5581 cggaatcatt gtcgatgaag gaatcaggga gggtgggaat tatggcccct atagacagag
     5641 cgaccggaaa actatttaca ggcaatatgc agataacctt atagataaag gtgatgcata
     5701 ttatgctttt gacactcctg aaaaacttga aacgctgaga agagaatcag aaaaggcagg
     5761 gaaaacattt atttataatg ctgaagtcag ggaaaaactg attaactcag tctctctaaa
     5821 ggagcctgaa tggagagaaa aacttgataa gggagaacct tacgttatac ggtacaaaat
     5881 gccctacaat gaagatattc gttttgatga tattatccgt ggacatatag ttgtaaatac
     5941 cggaaccctc gatgataagg ttttattcaa gtcagacggc atgcctactt atcatctggc
     6001 aaacatagtt gatgatcatc ttatgaaaat cagccatgta atccgcgggg aggagtggct
     6061 cccctcatta ccgctccata ttctgttata caggtctttt ggctgggatc ctcctctttt
     6121 tgcccacctg ccattactgt tgaagcccga tggaaaaggt aaactaagta aacgcgatgg
     6181 agataaaatg ggattccccg tttttcctct gttctggccg tacggcgaaa catctaaggg
     6241 ttatcgggag gatggatatt atcctgatgc gtttattaat atgcttgctt tgcttggctg
     6301 gaatccagga actgaacagg aattattttc aatggatgaa cttattaatt ccttttcaat
     6361 tgacagagtt ggtaagtcag gctccaggtt tgaccctgaa aaggcaaaat ggtttaatca
     6421 tcaatacctt caaaccaaaa ctaataatca gattgccata gagttcagag agtttctgag
     6481 ggcacgcggt tatcatattg atatcttaag acttgagtct cttgtaggaa tggtaaaaga
     6541 gagggtaagt ttcgtaaaag atatttggga acaga
//
LOCUS       DATGBN010000381         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_108779, whole genome shotgun sequence.
ACCESSION   DATGBN010000381 DATGBN010000000
VERSION     DATGBN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108779"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            589..2100
                     /locus_tag="VKL21_09485"
     CDS             589..2100
                     /locus_tag="VKL21_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMB46041.1"
                     /translation="METNFYKENVEKIAERFFYIAEQTGQLIFDGDVSTGTIEWSGAI
                     EEFTGYTPEEFSKFDLDACREIIHPEDRERVWSALMNSLKTGEKFEQKLRFKRADGNY
                     IYVEDSSIFLKDENNCVYRSIGLFKNITERKHDQEKLKVSEESFIRYLQNFREIGFQL
                     DSNFDIVLLHGAVKEITGYENEEFRSGKIRLDQLVDPEDLSGFLEKQRKLSDETSYLV
                     EQEYRIRNRDGNRVWVFESIQVVHYIDSANCFYQGFIQDITEKKIAMETFDRAEKIRK
                     KEIHHRIKNNLQVISSLLELERDNLLSGIPDREKIAEAFRESNNRIISMSIIHEELYN
                     SRDVETIDFASYLKKLTDDLFKSYIVGSSDIRLCLDMENLFLEMDYAIPLGLIVNELV
                     SNSLKYAFPDGRSGKISIELHRGVNDKNKILSSGTVGNNIQNSYCHCGFFTLIVEDSG
                     IGFPESIDFGNTRSLGLQLVNTLVDQIGGNIELEKGSGTRFKIWIPDSWGEVK"
BASE COUNT          930 a          420 c          581 g          834 t
ORIGIN      
        1 aatacagtac ttcaccaact ggatcagaaa atcatctatc cccattacga tttcaaacat
       61 ttaaccgaaa agtattttga aatcggaatg aagctaaatg atttctcgat ccagttggtg
      121 aagtactatt caataagata atttcaccta caaagtgttt ccatttaaag gaattagata
      181 gcatattact acttttgatt tcctttttat tttattatat ccttagaaaa gattttttgc
      241 aacggaaccc taattcatgt ctcaagcttt tttaattatt tatataagat aggctttatt
      301 tatgttaaaa aacatataaa tgtggggtta aaatgccaac tatacaaaca atgctacctt
      361 tggttaaatt cctcatctta ttaatggcag gagtggcact tgtaggagta atgctaggaa
      421 aatctaaacg cgaatgaaag gataaaggtg tctgagttat ttagtggtgg gttcttagct
      481 ctgttgaaaa tggtatgaag agtggtaaag ctacaataaa atttatgaag tatattttat
      541 aatttattac atgttctcaa ctttatatat tatcataaga ggaacatcat ggaaacgaat
      601 ttttacaaag aaaatgtgga gaaaattgcg gaaaggttct tttacatagc tgagcagacc
      661 gggcaattga tcttcgatgg tgatgtgagc actggtacga ttgagtggtc aggagccatt
      721 gaagaattta ccggatacac tccagaggaa ttcagtaaat tcgatttaga tgcctgcagg
      781 gagattatac atccagaaga cagggaaaga gtatggagtg cccttatgaa ctctttgaag
      841 accggagaga agtttgagca gaaattaagg ttcaagagag cggatggaaa ttatatttat
      901 gtggaagaca gctcgatttt cctgaaagac gaaaataatt gcgtatatag gagcatcggc
      961 ttgtttaaaa atattacaga aagaaaacat gaccaggaaa aacttaaggt aagcgaagaa
     1021 agtttcatca gatatttgca gaatttcagg gagattggat tccagctgga cagtaatttt
     1081 gatattgtgc tgctgcacgg agctgtaaaa gaaattactg gctacgaaaa tgaagaattt
     1141 cgttcaggaa aaattcggtt ggatcagctc gtggatcctg aagatctgag cgggtttctt
     1201 gaaaagcaac gaaaactgag cgatgaaaca agctatctag ttgagcaaga atataggatt
     1261 cggaacaggg atgggaatag ggtctgggtt tttgagtcta ttcaggttgt acactatata
     1321 gatagtgcaa attgttttta ccagggcttt attcaggata tcaccgagaa aaaaattgca
     1381 atggaaactt ttgatagagc tgaaaaaatc cgcaaaaaag aaattcacca cagaatcaag
     1441 aataacctgc aggtaatctc cagcctccta gaattagagc gtgataacct gctgtccggg
     1501 attcctgacc gtgaaaaaat tgctgaggca tttcgagaaa gcaacaatcg aatcatatca
     1561 atgtctatta tccatgaaga actatacaac tccagagatg tggagacgat tgatttcgct
     1621 tcttatctca agaaattaac agacgatctc ttcaagtcgt acatagttgg aagttctgat
     1681 atccgattat gtctagatat ggaaaattta ttccttgaaa tggattatgc aattcctcta
     1741 ggtcttattg tcaacgagtt agtttccaat tccttaaagt atgcatttcc agacggaaga
     1801 agtgggaaaa ttagcataga acttcaccgg ggagtcaatg ataaaaataa aattttaagc
     1861 agtggtactg ttggcaataa tatacaaaat agttattgcc actgtgggtt ttttacattg
     1921 attgtcgaag acagtggaat tggttttccg gaatctattg actttggaaa cacacgttcg
     1981 ctgggcttac agctggtaaa cactcttgtt gatcaaattg gtggtaatat agaacttgaa
     2041 aaaggatcag gaacgagatt taagatatgg attccagatt cttggggcga agtaaaataa
     2101 tattagctaa ggaatggtta agaaataact ccaagttttc actttgggaa tggctctata
     2161 attccatttc cccaaataaa catcaaagac aatatcggta acagaaatac ttgaaattat
     2221 agtttcatca ttcctaactg gaaatcttgg catgagattt ggggattttt taattttgca
     2281 ttgcgcatat gaattgtgtt taagatgtaa tcagtatcgc gtcgtgaggg taagttactg
     2341 aaatccgcaa accgaagtgt gattctcaaa tcaggacaaa catggcagat gtgccttttt
     2401 tgttagattt gaccaatagc ttatggacac gacccaatat aaaagttggt gataattgtg
     2461 acaacagccg aatataatac attgtgggaa aattactatt cgaactatag gaatgttgca
     2521 agtcctctca gaaagccaac tcttttcaaa tggctatata acagttatat aaatctgcac
     2581 ctttcttgtg tggatacatc tgaggtctta aggcttgccg acttcaagac atccctagtt
     2641 ttagtggata cagccgttga tgattcgtgt gattacgcgt cttttataga gaaaaacggt
     2701 ggagatcagt tcagttgcga gatgctaagg aatagataga aaataacttc aagttttcac
     2761 tttgg
//
LOCUS       DATGBN010000382         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_419529, whole genome shotgun sequence.
ACCESSION   DATGBN010000382 DATGBN010000000
VERSION     DATGBN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419529"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VKL21_09490"
     CDS             complement(<1..1118)
                     /locus_tag="VKL21_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HMB46042.1"
                     /translation="MRIRQKLISGFVIVSLLVGLVGFLGLYANNHIVTSFEKGEEHFG
                     SIIEASNEVSSYAKRAQGHTMLYLTLHNGTDKKKASDRIASLREQIAIIDGKIKDPDA
                     LVLLNDTKSRTNEMQYIIESLIEFHDNEMEQNGTFDFKNHETLIRNLDSIGSNIRQNG
                     LDLGKRELKLEEEINQNAKQKASSLYKFIFIISVIALISALIIGILFDRSISNPINKL
                     KNAAIKIRSGDFGTNIDIISEDEIGELSNEFNKMAQDLQKSSDQIISSGEYTDNIVNS
                     MNDSLIVTSQDGIIKRVNKATIFLLGYKKEELIGQHISSFIINGNKLLLDKCDIDKKN
                     INKSIETSFIPKGKKEIRILLSSSIMYDKNGDIQDIVCV"
     gene            complement(1270..1671)
                     /locus_tag="VKL21_09495"
     CDS             complement(1270..1671)
                     /locus_tag="VKL21_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HMB46043.1"
                     /translation="MILDYKDIAEYIINNYKNKVVEVGVGSLPQVALLLKDKLDVVVT
                     DVNEQKYAGVRFCRDDIFKPDMGIYRNASLIYSIRPPIDLQDAIAAIGKEVGADLIIR
                     PFGNEKADLRKYFKEHSIMNYKKARFYLYRS"
     gene            1899..2165
                     /locus_tag="VKL21_09500"
     CDS             1899..2165
                     /locus_tag="VKL21_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46044.1"
                     /translation="MKNEEKEKLLEKARKAKDEIAFFERKGNKILSEIFEKSNWYPEC
                     HNNPDKKECDHCMNCWPKDCNGSNCDRVGRCMCPTTECICLGRY"
     gene            2229..2795
                     /locus_tag="VKL21_09505"
     CDS             2229..2795
                     /locus_tag="VKL21_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HMB46045.1"
                     /translation="MVNTIDPKKTIKIENVVASTAIGAKLDLNEVIRVLEGADYNKER
                     FPGVVYRTTSPKTAALIFGSGKIVCTGAKSIADVGVGLSKVFEKLKTMGVDIPTKPEI
                     TIQNIVASADLGRVLNLNAIAIGLGLENIEYEPEQFPGLVYRLSVPKVVMLLFGSGKL
                     VVTGGKKPEDAEAAVEKIVLELDGLGLL"
     gene            2892..>3500
                     /locus_tag="VKL21_09510"
     CDS             2892..>3500
                     /locus_tag="VKL21_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMB46046.1"
                     /translation="MRVLLIGGGGREHAIAEAVARSLNNPELFAAMSKKNPGIARLCK
                     DFLLIKETDPEVVNFAIKNNIELAIIGPEAPLAAGISDMLWEAGIPVVGPRRLAAQIE
                     FDKAWTRDFMKKYGISGCPGFKVFRKGESGTDEFIDELGDVVIKPAGLTGGKGVRVMG
                     DHFGIEGSKTYAKEVLKNDDVVIEERLIGEEFTVQAFVDGENL"
BASE COUNT         1077 a          596 c          699 g         1128 t
ORIGIN      
        1 acacatacta tgtcctggat atccccgttt ttatcataca tgatagaaga ggaaagaaga
       61 atccttattt cttttttgcc ttttggaatg aatgatgttt ctatactctt gttaatattc
      121 ttcttgtcga tatcacactt atcaagcagc aatttattac cattgattat aaatgagcta
      181 atatgttggc cgatcagctc ttctttcttg taaccaagaa ggaagattgt ggctttgttt
      241 actcttttga tgatgccatc ctgtgaagta acaatcaggc tatcgttcat ggaattgacg
      301 atattgtccg tatattcccc ggatgagatt atttggtcac ttgatttttg cagatcttgc
      361 gccattttat tgaattcatt tgaaagttct ccaatttcat cttctgatat aatatcgatg
      421 ttagttccga aatcaccact tcttattttg atagcagcat ttttgagttt attgattgga
      481 tttgaaatac tcctatcaaa taatattcca atgatcaaag cgctaataag agcaattaca
      541 ctgattatga aaataaattt gtataatgaa gatgcttttt gttttgcatt ttgattaatt
      601 tcttcttcca gtttcagttc tctctttccg agatcaagac cattttgacg gatgtttgag
      661 ccaatactat ccagatttcg tatcaatgtc tcatgatttt taaaatcaaa cgtcccgttt
      721 tgttccattt cgttatcatg aaattcaatc aatgattcta taatatattg catctcattt
      781 gttctggact ttgtatcgtt taacaacacc agggcgtcag gatcttttat tttgccatct
      841 attattgcta tttgttccct cagagatgcg atcctatcgg aggctttctt tttgtcagtc
      901 ccgttatgca atgtcaaata tagcattgtg tgaccctgtg cccgttttgc ataactgctt
      961 acttcatttg aggcttctat tatactcccg aaatgttctt cacctttttc gaaggaagtt
     1021 acaatgtggt tattggcata aagaccaaga aatccaacca gacctaccaa taatgatact
     1081 ataacgaatc ctgatatcaa tttctgtctt attctcattt aagcaccgat ttatttacca
     1141 ataatattga atcattattt ttgatatgga tattatttac attttgattt aattggtaga
     1201 taaaaggatt tgtgaataat gtgtgaataa ttagtgaata atgcttgaat aattcgttca
     1261 gtgtttggct tatgacctgt ataaatagaa cctggctttt ttgtaattca taatagaatg
     1321 ttctttaaaa tattttctca aatccgcctt ttcgttcccg aatggcctta tgataagatc
     1381 agcccccact tctttgccaa ttgctgcgat cgcatcctgc aggtctatgg ggggtcttat
     1441 tgaataaata agggatgcat tcctgtaaat tcccatatcg ggtttgaaaa tatcatccct
     1501 gcaaaatctt actcctgcat acttttgttc attgacgtcc gtgaccacaa catccaattt
     1561 atccttcaag agaagagcaa cctgtggaag gcttccaaca cctacttcta ctactttatt
     1621 cttgtaatta ttaatgatat attctgcaat atctttgtaa tcaagaatca ttggtatgat
     1681 taacaatgta atgatttaat aagctattgt aataaatatt catttacaat tttcgaatga
     1741 gtccacaagc cttttaaaga tgtagtacca tatgataatc ataatgagta caatataata
     1801 atctatcacc attaatcata actgggatag cataacaaat tgaatgttca atggttgtga
     1861 caaaaaagac agacagaata ttaaatcagg tgattgatat gaaaaatgaa gaaaaagaaa
     1921 aattgttgga aaaggcaaga aaagcaaaag atgaaatagc attctttgaa cggaaaggaa
     1981 acaagatctt atccgagata tttgaaaagt caaactggta tccggaatgt cataataatc
     2041 ctgataaaaa ggaatgtgat cattgtatga actgttggcc aaaagattgc aatggatcaa
     2101 attgtgatag agttggcagg tgcatgtgtc caactacgga atgtatttgt cttggaaggt
     2161 attgaaaatc ctttccagtt taccccaatc tatataaatc tttaaatacc ctatataaag
     2221 gtgagaatat ggtaaatact atagatccaa aaaaaactat aaagatagaa aatgttgttg
     2281 catcaactgc tattggggca aaacttgatc ttaatgaggt tatcagggta cttgaagggg
     2341 cggattataa taaggagaga ttccctggtg ttgtttacag gactacgtcc cccaaaaccg
     2401 ctgcgcttat attcggaagt ggaaagatcg tatgcacagg agcaaagagt attgctgatg
     2461 taggagttgg tctttccaag gtttttgaaa aattaaagac aatgggtgtt gatattccca
     2521 caaaaccaga aataacaatt caaaatatcg tggcatcagc cgatctggga agagtgctga
     2581 acctcaacgc catagcaatc ggccttggac ttgagaatat cgaatatgaa cctgaacaat
     2641 tccccggtct tgtttacagg ctgtccgtac ctaaagtagt catgctttta ttcggttcgg
     2701 gtaaacttgt tgtaacaggg ggaaagaaac ccgaggatgc cgaagcggca gtagagaaga
     2761 ttgtgctgga acttgatgga ttgggtctgt tataacttaa aacgatcata acatcggaca
     2821 ggatttcttc aagcttgcta ataattggat gaaccgaaca tttttatttt taaacatatt
     2881 ctgggatacc tatgagagtc cttttgatcg gaggcggagg gcgggaacac gctattgcgg
     2941 aggccgtcgc acgaagtctt aataatccgg aactttttgc cgccatgagt aaaaaaaatc
     3001 cgggtattgc gcgtttatgt aaagatttcc tgcttataaa agaaacagac cctgaagttg
     3061 taaattttgc gataaaaaat aatattgaac ttgccataat aggacctgaa gctcctcttg
     3121 ccgctggcat ttctgatatg ctatgggaag ccgggatccc ggtcgtaggg cctcgccgcc
     3181 ttgcggcgca gatcgaattt gataaggcat ggacaaggga ttttatgaag aaatacggga
     3241 tttcaggatg tccgggtttt aaagtattcc ggaaggggga aagcggtacg gatgaattca
     3301 tagatgaact cggggatgtc gtgataaaac cggcaggtct cacgggtggg aaaggagtaa
     3361 gggtcatggg agaccatttt gggattgaag gttcaaagac ctatgcaaaa gaagtcctga
     3421 aaaatgacga tgtagtgatc gaagagcgcc tgatcggtga agagtttacg gtacaggcat
     3481 ttgtggatgg ggaaaatctt
//
LOCUS       DATGBN010000383         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_496820, whole genome shotgun sequence.
ACCESSION   DATGBN010000383 DATGBN010000000
VERSION     DATGBN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496820"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..2107
                     /locus_tag="VKL21_09515"
     CDS             416..2107
                     /locus_tag="VKL21_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB46047.1"
                     /translation="MACASFFPEEKERPFVDREEFIQAFYNALETIKGKEAKILVYHG
                     VAGIGKTRLRREISKIIEKYNDSNRNFRIYNESNSNFHILCATVDFETEEYRQSDKFL
                     EILRDQFQGEYGIKFHTFDFAHALYWKKVNPRIPLCKENDSEDNIFTHLLDIGDDLSN
                     IGGIFNPVNVSIKMAKLIKRLPASYREWELKNRNEISRLISMEAAEIRRQLYLYWTYD
                     LREYLQRNSESAVIFIDSYEALWGNDRSLASLSSKDEWLRKEIQTLLSSSCLWILCGQ
                     ESPRWVEDSNTEKAIWGNSIERHEIKGLPEEHARNLLKHSDVLEKYIQTAIINGSEGV
                     PYYLDLSIDLYRIIKKIREPEPGDFAKVPPDIFKRFIKYLDEPKEAAIKVLSVPRFWN
                     REIFNVLIKEFTSYPKNEMSRIHNFSFINKDEIENNKWSMHQLMRKSWQEYQEKEKNE
                     EHDQRKSVHEFMLSYYVNKLENLDIKKITREHKVALIEAFYHAKESLEAEDLLRNDEN
                     IISNISVTDIIFDGYLGKWNNRVIPKVKNLKLFFNHSLIGSLLFQTRLIEQHSGN"
     gene            2116..>2489
                     /locus_tag="VKL21_09520"
     CDS             2116..>2489
                     /locus_tag="VKL21_09520"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMB46048.1"
                     /translation="MHEEILQIIEAEPELGPEHPDVATTLNNLAALYYNMGDYEQALP
                     LYQRALDISEKVLGPHHPSVATSLNNLAGLYRHMGDYDKALPLFQRALDIREKVLGPQ
                     HADVATSLNNLALLYHHMGDYE"
BASE COUNT          865 a          455 c          458 g          711 t
ORIGIN      
        1 tataacctat gcataattgc cttttttacc ctaaaaaatg agtaaatata gccttaaaac
       61 tcctatttta cataggtttt acaagaaact gtgtaccaac atactcctta aacgttggta
      121 ttttcttcaa aataaggtga aaaacctcaa aaaagacggt aatacccatg aaataccaac
      181 gttttatcag aaaacgtagc cattttctag ttttggcagt gtgtcgaatt tctagataac
      241 aacttattga catttgtact tttacagaat tcttgatacg ctgcctttta atctgttaat
      301 gctaatttag agaggattta cacagcgtcc aaatttcgat agtttattat agaattctta
      361 aagcataatt cctttatatt tttctggttc acagtacaga ataaggagta atctaatggc
      421 atgtgcttca tttttccctg aagaaaaaga gagaccattt gttgatagag aagaattcat
      481 ccaagcattc tataatgccc ttgaaaccat aaaggggaaa gaagcaaaga ttctagtcta
      541 ccacggtgtt gctggtattg gtaaaacacg cctaaggaga gaaatatcta aaataattga
      601 gaaatacaat gattcaaaca gaaacttccg aatatacaat gagtcaaata gtaactttca
      661 tattttatgt gcaacggtag atttcgaaac tgaagagtat agacaatctg ataagttcct
      721 agaaatattg agggatcagt tccaaggtga atatggtatt aaattccata cctttgactt
      781 tgcccatgct ctttactgga aaaaagtaaa tccaagaatt cctttgtgca aggaaaacga
      841 ttctgaagat aacatcttta ctcatttact tgatattggc gatgacttat cgaatattgg
      901 tggaatattt aacccggtaa acgtatcgat taagatggca aagctcatca aaaggcttcc
      961 tgcttcatac agggaatggg aattaaaaaa tagaaatgaa atttcccgat taatcagcat
     1021 ggaagcagca gagatcagga ggcagcttta cctttactgg acttacgacc tgcgtgaata
     1081 cctccaaaga aactctgaat ctgcagtcat ttttatcgac agctatgaag cactctgggg
     1141 aaatgacaga tccctagcaa gtttaagttc caaagacgaa tggcttcgaa aggaaataca
     1201 aactctcctt tcctcctctt gcctctggat actctgtggg caggagtctc ctcgatgggt
     1261 ggaagattcg aacaccgaaa aagccatatg gggaaattca attgaacggc atgaaattaa
     1321 aggtctgcca gaagaacatg cccgtaactt actcaaacac tctgatgttc ttgaaaaata
     1381 tatccagact gctattatca atggcagtga aggcgtgccc tattacttag atctctcaat
     1441 cgatctatat agaattataa agaaaatccg cgaaccagag ccaggtgatt ttgcaaaggt
     1501 gccgccagac atatttaagc gttttataaa atatctcgat gaacctaaag aagcggcaat
     1561 taaagtcctc tctgtacctc gtttttggaa ccgcgagata ttcaatgtcc tgattaaaga
     1621 attcacaagt tatccaaaaa atgaaatgtc taggatacat aatttctcct ttattaataa
     1681 agatgaaatc gaaaacaata aatggagtat gcaccagctg atgagaaaga gctggcaaga
     1741 atatcaagag aaggaaaaga atgaagagca tgatcagagg aaaagtgttc atgagttcat
     1801 gcttagctat tacgtaaata aactggaaaa tctggatata aaaaagatta ctcgagaaca
     1861 taaagttgct ttaatagaag ctttttatca cgcaaaagaa tcactggaag cagaagatct
     1921 gcttaggaat gacgaaaata taatttcaaa tatttcggtt accgatatta tttttgatgg
     1981 ttatttgggg aaatggaata atagagtcat tcccaaagtg aaaaacttga agttgttttt
     2041 taaccattcc ttaattggtt cattactgtt tcagacccgt ttaatagagc agcattctgg
     2101 caattaattg ctcctatgca tgaagaaata ctgcaaatta tagaagcaga accagaactc
     2161 ggacctgaac atccagatgt tgctacaacc ctaaacaatc tcgctgcact ttattacaat
     2221 atgggagatt acgaacaagc actcccactt tatcaaaggg cacttgacat tagtgaaaaa
     2281 gtgctgggtc cgcaccaccc atctgttgca acttcactaa acaatctcgc aggactctat
     2341 cgtcatatgg gagattacga taaagcactc ccactttttc aaagggcact tgacattcgt
     2401 gaaaaagtac tgggtccgca gcacgcagat gttgcaactt cactaaacaa tctcgcttta
     2461 ctctatcatc atatgggaga ttacgagaa
//
LOCUS       DATGBN010000384         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_458267, whole genome shotgun sequence.
ACCESSION   DATGBN010000384 DATGBN010000000
VERSION     DATGBN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458267"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1028)
                     /locus_tag="VKL21_09525"
     CDS             complement(21..1028)
                     /locus_tag="VKL21_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HMB46049.1"
                     /translation="MNKLTKAVRVRVEDSESVRQKLISLSALDKSRKLLKKDGFIEIP
                     VTENFITNEFTLIYQDNPEYYLKEKTVFNDLDIPENEKKLLPRGWQILGDIIIVTLDE
                     KLMDRKVKIGKALLSYYPGCKTVLLDRGISGQMRKPDREIIAGEDITETVQKENGCLF
                     KLDAMKVMFSQGNLAEKKRMSKLGKDETVVDMFAGIGYFSIPIAVHANPKKIISIEIN
                     PVSFGYLNENIVLNRVGDIIEPIAGDCAKVTPRRIVNRVIMGYLDGQDYLETGINALL
                     PGGILHYHEAVPEAIESRPVDRIIEVSRKIGKNVEITGRRRIKKYAPGVWHVVVDARV
                     D"
     gene            complement(1384..>1765)
                     /locus_tag="VKL21_09530"
     CDS             complement(1384..>1765)
                     /locus_tag="VKL21_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB46050.1"
                     /translation="SKIISIPGIGDTLGPMILGEIGDAERFSTAKKLIAFAGLDPIVS
                     QSGRFENKSGKISKRGSPLLRKGLFLAANVAIQNDENLKRFYDKKRSEGKHHFSALNA
                     VAAKLLRITYWVLKNNKEYQTQPN"
BASE COUNT          471 a          425 c          298 g          571 t
ORIGIN      
        1 aaaaatatgt acgttaagga tcaatccacc ctcgcatcca ctaccacatg ccagaccccg
       61 ggcgcgtatt tctttatcct gcgccgccct gttatttcaa cattttttcc aatcttcctg
      121 gaaacctcga ttatcctgtc aacagggcga ctctctatcg cctctggcac tgcttcatga
      181 taatgcagga tcccgccagg cagcaacgcg ttaatcccgg tttccagata atcctgtcca
      241 tccagatatc ccataataac ccggttcaca attcttcgcg gcgttacttt tgcacagtct
      301 cctgctattg gttcaataat atccccaacc ctgttaagaa caatattctc gttcaaataa
      361 ccaaaagaaa caggatttat ctcaatagag attatttttt tggggtttgc gtgtactgct
      421 attggaattg aaaaataccc gatgcctgcg aacatatcga ccactgtctc gtcttttccc
      481 agtttactca tgcgcttttt ttcagccagg tttccctgtg agaacatgac cttcattgca
      541 tctagcttaa aaaggcatcc attctctttt tgtacggttt cagtaatatc ttctccggcg
      601 atgatctcac gatcaggctt cctcatctgg ccgcttattc ccctgtcaag aagtacagtc
      661 ttgcatcccg gataatatga aagcagggct tttcctatct tcacttttcg atccattagt
      721 ttttcatcaa gtgttacgat gataatatca cccaggatct gccatccccg cggcaataat
      781 ttcttttcgt tttcaggaat atcaaggtcg ttgaaaactg ttttttcctt caagtaatat
      841 tccggattat cttgatagat cagtgtaaat tcatttgtta tgaaattttc agtgactggg
      901 atctcgataa aaccgtcctt tttcaataac ttccttgatt tatcaagagc tgacaaggaa
      961 ataagttttt gccggactga ttccgaatcc tccactctga cgcggacagc tttggttaat
     1021 ttattcatgt gacattaatt ggtttagttc cccaatgacc tgcttgggaa tcattgaata
     1081 ttcaccgcaa tgttcgcaaa agattaacac atctatgctt tgcgttcttt taattttttg
     1141 gattatattg ctcaatgaaa cgaatattaa ccgcagataa gcgtactatt aacactttcg
     1201 gtcattatta tctttactgc gctggggttg caaccccaga ccccaatgga agcgccgccg
     1261 ctggcggggt ttaacatgaa ctcaccggtt tcataaatca caactattca tacttgcaat
     1321 gtgtaataaa atagtatcca ctaactattt gtttggctac atccccacgt gacaaaaatc
     1381 tgattaattc ggttgtgtct gatactcctt gttattcttc aagacccagt acgtaattct
     1441 caacaattta gcagccaccg cattcaaagc agaaaaatga tgctttccct cgcttctctt
     1501 tttatcataa aagcgcttca aattctcatc attttgtatt gccacattag cagcaagaaa
     1561 aaggcctttt ctaaggagtg gcgaacctct ttttgagatc tttcctgatt tattctcaaa
     1621 ccttcccgat tggctgacaa taggatcaag cccggcaaaa gcaatcaatt tcttagcggt
     1681 agaaaacctt tcagcatccc cgatttcacc aagaatcatg gggcctaaag tatctccaat
     1741 tccaggtatt gaaattattt ttgaa
//
LOCUS       DATGBN010000385         6949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_148038, whole genome shotgun sequence.
ACCESSION   DATGBN010000385 DATGBN010000000
VERSION     DATGBN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148038"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..326)
                     /locus_tag="VKL21_09535"
     CDS             complement(30..326)
                     /locus_tag="VKL21_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HMB46051.1"
                     /translation="MHEISIAGAIIDSVLDAARKNNAIKVKEVAGTIAEGATYNIKVM
                     KALINCKKCAYNGPIEFYEKLHFFLPVIKCPRCDESEVDIVAGRECCVNKINIS"
     gene            complement(335..694)
                     /locus_tag="VKL21_09540"
     CDS             complement(335..694)
                     /locus_tag="VKL21_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46052.1"
                     /translation="MLGRALRCCIAYAGVVAEVHAGIAGPKWGALFKEYCSICPMKLC
                     RKGKNTGEEANNLLVSLERRNLKTDVRRDTYITAMVKKDGALVGEGIGTVCAFSSLMY
                     AKARCIQLRSEIEVVRE"
     gene            951..1118
                     /locus_tag="VKL21_09545"
     CDS             951..1118
                     /locus_tag="VKL21_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMB46053.1"
                     /translation="MGAIGSGKTLLIEKTIGQLGPNYRIGAIAGDVVARLDASRFKKL
                     GYCRSSKCRPG"
     gene            complement(1228..2217)
                     /gene="dph2"
                     /locus_tag="VKL21_09550"
     CDS             complement(1228..2217)
                     /gene="dph2"
                     /locus_tag="VKL21_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HMB46054.1"
                     /translation="MKNHFDFDLGRIKEIIKENHHRTVGLQFPEGFKRRAIMIAGELE
                     DDTGSDIIISGNPCFGACDIDTTLAGKVDVMFHFGHSKMGKFENVVFIEARSSVDVLQ
                     CVKIALPIFKTKKIGLITTVQHIHKLEEIARFLDENGKKGISGKGDIRVNYPGQVLGC
                     NFSAARLECEEYLYIGSGIFHPLGVAIATGKKVVAADPYLNQVIEVSPEKFIRKRSGY
                     IAKSMDAKVFGVVVSSKSGQNRIDLARKLSGMARRHGKKAQIIMMDLVTPEQLLAFKV
                     DAYVNTACPRITIDDAERFHVPVLSPSEFEIVLGERKWDSLEMDEILGDEGQT"
     gene            complement(2214..2861)
                     /locus_tag="VKL21_09555"
     CDS             complement(2214..2861)
                     /locus_tag="VKL21_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HMB46055.1"
                     /translation="MRLDSYLVEIGNVGSRGRAKRAIIEGNVRVNDRIITKSSFNVAY
                     SDKIEITEDLDKPAGYWKLKDIQEKTNIIKESDNVLDVGSSAGGFLLYASEIASHVHG
                     IEFSHEFRSILGKIAHERPNISIEFKDVFTMAHDNEKYDVLLFDITVNPLDSLKALGN
                     VYPSLKRGGMLLQVFKLPEKNDIGPMLEGLSLIGFEIIRIIEAQKKEAYVIAKKI"
     gene            2988..4148
                     /locus_tag="VKL21_09560"
     CDS             2988..4148
                     /locus_tag="VKL21_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HMB46056.1"
                     /translation="MNVDRTIKLKLDLSEYDKETLKLTMSMSNHVFNEIARYGFENHT
                     SSKVSIHHATYYDIRGRYPELPCSILQGIRDVACEALKGVELKKLPKSKQFSAIRYNK
                     RVCNINLLEQTVTLASIKGRVKATFPIPEYYKQYLVWEIRTSTLGYNKQQDMFYLHVT
                     LRKPSPEPTRDNEVLGIDRGIVNIAVISSNDFFNSKSIKNVRAKYAYLRAKLQSKGTR
                     SAKKLLRKISRKEERFVTWVNHNISKEIAAMPYDVLAIEDLTSIRVQSKTKGVKFTKK
                     LNNWSFYQLEQFLRYKAEAFGKSVITIDPRYTSQKCSKCGHIYKGNRKGNSFHCVKCG
                     FQIHADLNAARNIAILGKSGNSRRTVTSPYIACDDAEARKGIDAEHSYNART"
     gene            4210..4295
                     /locus_tag="VKL21_09565"
     tRNA            4210..4295
                     /locus_tag="VKL21_09565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4244..4246,aa:Ser,seq:cga)
     gene            4351..5226
                     /gene="ligD"
                     /locus_tag="VKL21_09570"
     CDS             4351..5226
                     /gene="ligD"
                     /locus_tag="VKL21_09570"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HMB46057.1"
                     /translation="MDDLTKVSFTNLEKILFPELELKKSRVIEYYIRIAPLMLGSLKN
                     RIIVMNRYPDGINKDGFYEKDAPKGIPSWVETFNTYSETAQREIDYIICNNLDTLIWM
                     ANLAALEINITLSTIDNYDKPDLVFFDIDPEPPCSFDDVMDIALLLKEKLDKLNLRSF
                     VKTSGKKGLHVVLPIINNYNFKQTREFVHQFGKFLAKESDLVVSEFRNSRDPGTVFID
                     YLQNTRGKTMIAPYSLRAQPQATVSTPLEWKELKKGLRPEEFNIFTLIKRKNDPWEGL
                     MESKQSLEVEYNGNG"
     gene            5213..6010
                     /locus_tag="VKL21_09575"
     CDS             5213..6010
                     /locus_tag="VKL21_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HMB46058.1"
                     /translation="MEMDKGSAKPSHRPIWSGSITIGLVNVPVKLYTMIRDQAFSFRL
                     LHNMDGQPLKYERVCIKDDKVVDWKDTVKGYEIRKDEFIVFKKEELDAIRPESDERIR
                     LDKFVSLLSIDTVYFDKSYILVPDRSEEAYSLMMTVIAQLGKAGMGKVTMRTKEYPVV
                     VHEYNGALILTTLRYANEVVNPAGMEELSQLKKPGKKEMELAEKIINDLSGEFDITEY
                     RDSYREKMEQLIEMKRKGEVVVAQVPKKEEVKELMATLQETIKRLSK"
     gene            6007..>6949
                     /gene="ligD"
                     /locus_tag="VKL21_09580"
     CDS             6007..>6949
                     /gene="ligD"
                     /locus_tag="VKL21_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HMB46059.1"
                     /translation="MKQYKAMLARPADAPFSSDDWIFEVKWDGIRAISYVKEELSIRS
                     RNQKELIANFPELSELKDLARDMVLDGEIIVMKDGKADFATLIKRSQNTKAMDIDHMA
                     RKFPATYIIFDILEKKGEELLDIPLIERKKILENSLKEGQFVVLSLFVEGSGVDYYRA
                     ALEKGIEGVMAKKKQSPYEPGKRSNYWLKIKQVKTCDCVIFGYTKGEGNRDETFGSLI
                     LGLYDMERPVFIGKVGTGFSREIMENLKRFLDEYKVEEETLQGVDMDREITWLRAGVV
                     CEVGYQSITEDQKLRIPVFRNIREDKDPFECTIDQIRP"
BASE COUNT         2060 a         1466 c         1501 g         1922 t
ORIGIN      
        1 tttcccgctt atcttataat tatatatttt caagatatgt ttattttatt gacacagcat
       61 tcccgtcccg ccacaatatc tacctcagat tcatcgcatc gtggacattt tattaccggc
      121 aggaaaaaat gtagtttttc atagaattct atcgggccgt tataggcaca tttcttgcag
      181 tttatcaagg ctttcatgac cttgatatta taggtagccc cctctgcaat agttcctgcg
      241 acctctttga cttttatggc attatttttt cttgcagcgt cgagcactga atcaattatt
      301 gctcctgcta tggatatttc atgcattcag ttcactattc ccttacgacc tctatttcag
      361 accgaagctg gatgcatctg gctttagcat acatcaatga tgaaaatgca cagacagtcc
      421 cgatgccctc tcccacaagc gcaccgtctt tttttaccat agcggtaatg taggtatccc
      481 tcctgacatc tgtcttcaaa ttcctccttt caagagaaac aaggagattg tttgcttcct
      541 ctcccgtatt ttttcctttc ctgcataatt tcatgggaca tatactgcaa tattccttga
      601 ataatgctcc ccatttgggt ccggctatac ctgcatgaac ttccgcaaca acacccgcat
      661 aggcaataca gcatcttaaa gccctgccca gcacctgtcc tttttcaatt atctcacagc
      721 atatccttta gctcttcttt tatttttgcc acaagagccg gcgcgcgcgg tatggtcgcc
      781 gctgtgccgc atgtgggaca atatgatatt gggccgactg ccatttcaat gtcatcttta
      841 tcgatgaata ctacctatcg atcggatatg atgttctggc ggcaaacgca aagcttgcga
      901 gaaacaaccg gatcaccctt gagaaattcg gaattacagc tttcaatatc atgggcgcaa
      961 taggctcagg aaaaacattg cttatcgaaa agaccattgg gcagctgggt cccaactaca
     1021 ggataggcgc catagcaggg gatgttgtag cgcgtcttga tgcttcacgt tttaaaaaat
     1081 tgggatattg cagaagcagt aaatgcagac ccggataaaa tggtaaggga tgcgcgcatc
     1141 atcaatccgc gtattgatgt cctgaagatg agtctcaaaa caggagaagg gttcaatgaa
     1201 tggatagaat tcatgaaaaa aagtatctca ggtttgaccc tcatctccta ggatctcatc
     1261 catttccagc gaatcccatt ttctctcacc caggacaatt tcaaactccg aaggcgacag
     1321 gacaggtaca tggaatcttt cagcatcgtc gatagtgatc ctggggcatg ctgtattgac
     1381 gtatgcatcc accttgaatg ccagtaattg ttcaggggtc acaagatcca tcataattat
     1441 ctgcgctttc tttccatgcc tgcgggccat tccggaaagt tttcgtgcga gatctatcct
     1501 gttctgcccg ctttttgaag aaacgacgac tccaaagacc tttgcatcca ttgactttgc
     1561 aatatacccg cttcgctttc gtatgaactt ttccggggat acttctatga cctgatttaa
     1621 gtaggggtct gccgcaacta ctttcttgcc agtggcgatc gccactccga ggggatggaa
     1681 aatcccgctg ccgatatata gatattcctc gcattcaaga cgcgccgccg aaaaattgca
     1741 gccaagcacc tgtcctggat aattaactct tatatctcct tttcctgaaa tccctttctt
     1801 tccattttca tccaggaatc tggctatctc ttccagttta tgtatgtgct gtaccgtagt
     1861 tatcaggccg atcttttttg tcttgaaaat aggaagtgcg atttttacac attgcaggac
     1921 atcaacactg gagcgcgctt ctataaaaac cacattttcg aatttaccca tctttgaatg
     1981 cccgaaatgg aacatgacat cgactttacc cgcaagagtc gtatcgatat cgcatgcccc
     2041 aaaacaggga ttgcccgata tgattatgtc tgagcccgta tcatcctcaa gttctccggc
     2101 gatcataatg gcccttctct taaaaccttc gggaaattgg agtcccacgg ttctgtggtg
     2161 attttccttt attatctctt ttattcgccc tagatcaaaa tcgaaatggt tcttcatatt
     2221 ttctttgcaa tgacgtacgc ttcttttttc tgcgcttcaa ttattcgtat gatctcgaac
     2281 ccaattaagg ataatccctc caacatcggc ccaatatcat ttttttcagg taatttaaaa
     2341 acctgaagaa gcattccgcc tcttttcaat gaaggatata cattcccaag agcctttaat
     2401 gaatccaaag gatttaccgt aatgtcaaaa agcaagacat catatttttc attgtcatga
     2461 gccattgtga aaacatcctt gaattcaatt gaaatattcg ggcgctcatg tgcgattttt
     2521 ccaagaatgg agcggaattc atggctgaat tcgatcccat gcacgtgtga ggcgatctct
     2581 gatgcataaa gcaggaaacc tcctgcgctt gagccaacgt ccaggacatt atcactttcc
     2641 ttgatgatat tcgtcttctc ctgtatatct ttaagtttcc agtagccagc aggcttatcc
     2701 aggtcttctg taatttcaat tttatccgaa tatgccacat tgaatgaaga tttggttatg
     2761 atcctgtcat ttaccctgac atttccttcg attattgcgc gttttgccct gcccctggaa
     2821 ccaacatttc cgatctccac gagataggag tcaagcctca tgaaacacct ttttcacata
     2881 tatcatttgc tcctccatgg ggtgcggaga tcctgcattt caatgcaggg aggagcgtac
     2941 cccgcctccc aataccataa actaattatg tttatgagtg ataatatatg aatgtggaca
     3001 gaacgataaa gcttaaattg gatttgtctg agtatgataa agaaactctc aaacttacaa
     3061 tgtctatgag caatcacgtg tttaacgaaa ttgccagata cggttttgag aaccatactt
     3121 ccagcaaggt atctatccac catgcgactt actacgatat tcgtgggagg tatcctgaac
     3181 tgccctgttc tatcctccag ggcatcaggg atgttgcctg tgaagctctt aaaggagttg
     3241 aacttaagaa gcttccgaag agtaaacaat tttccgctat caggtacaac aagagagttt
     3301 gcaacatcaa tcttcttgaa caaactgtta ctcttgcttc tataaaaggc agagtaaaag
     3361 ctacttttcc aattcctgaa tactacaagc agtatcttgt atgggaaatt agaacatcca
     3421 ctcttggata caataaacaa caggatatgt tttacctgca tgtgactctc agaaaaccat
     3481 cgcccgaacc taccagggat aatgaggttc ttgggattga tagggggata gttaatattg
     3541 cagttatttc cagtaatgat ttcttcaaca gcaaatctat caaaaatgtg agagcaaagt
     3601 atgcatacct tagagcgaaa ctccagagca aaggcactcg ttctgctaag aagcttcttc
     3661 ggaagattag cagaaaggaa gaacggtttg taacctgggt gaatcacaat atctctaagg
     3721 aaattgcggc tatgccttat gatgtgctcg ctattgaaga ccttaccagc ataagagttc
     3781 agagcaaaac gaagggagtt aagtttacca agaaactcaa caactggagt ttttaccagc
     3841 ttgaacagtt tttgagatat aaagctgagg cttttggtaa atctgtcata accatcgacc
     3901 ctcgttatac cagtcagaaa tgttctaaat gcggacatat ctataaagga aacaggaaag
     3961 gcaattcttt ccactgtgtt aaatgcggat ttcaaataca tgctgacctc aatgctgctc
     4021 gtaacatcgc tatcctcggt aaatctggga atagtaggcg aactgtaacc tcgccataca
     4081 tagcgtgtga tgatgctgaa gcccgcaagg gaattgatgc tgaacatagt tataatgccc
     4141 gcacttaagt gcggggtagg ttactccaaa ggttaatagc aaattaacta tattaaaagc
     4201 ataccccatg ccgaggtggc agagcggact aacgcggcag gctcgagacc tgttttacct
     4261 tccggtaagc tagggttcaa atccctacct cggcgtcaat ttttcaaatc ggataaaaat
     4321 ttttatagcc tttttgataa tataatcaat atggacgatc tcactaaagt atcattcacc
     4381 aatctggaga agatcctctt tcccgagctg gagttgaaaa aatcccgggt catcgaatat
     4441 tatatcagga tcgccccgct gatgctggga tcactaaaga ataggatcat agtcatgaac
     4501 aggtaccctg atggcattaa taaagatggg ttctatgaaa aggacgcgcc aaaagggatt
     4561 ccgtcctggg ttgaaacttt caacacatac tcagaaacag cacagcggga gatcgattat
     4621 atcatatgca ataacctgga tacccttatc tggatggcga acctggccgc acttgagata
     4681 aacatcaccc tatcaacaat tgataattat gataaacctg accttgtgtt ttttgatata
     4741 gatcctgaac ctccctgttc ctttgatgat gtcatggata tcgctctatt gctgaaagaa
     4801 aagctggata aactaaactt gaggtcattt gtcaaaacct caggcaagaa agggctgcat
     4861 gttgtccttc ccattatcaa caattataat ttcaagcaga caagggaatt cgttcatcaa
     4921 tttggaaaat ttcttgcaaa agagtctgac ctggttgttt cagaatttag aaactccagg
     4981 gatcctggaa ctgtttttat agattacctg caaaataccc gcggtaagac catgatcgcc
     5041 ccatatagcc tgagggcgca accgcaagca acagtatcta caccgcttga atggaaggaa
     5101 cttaagaaag gattaagacc tgaggagttc aacatattta ctttaataaa aaggaagaac
     5161 gatccctggg aggggttaat ggaaagtaaa cagtcactgg aagtggaata taatggaaat
     5221 ggataaaggc agcgctaaac cttcgcacag gcccatatgg agtgggagta taaccatcgg
     5281 gctggtcaat gtaccggtca agctctatac catgatccgt gatcaggcat tttctttcag
     5341 gcttcttcat aatatggatg ggcagccatt gaaatacgaa agagtctgca ttaaagatga
     5401 taaggtagtg gattggaaag atacggtaaa aggttacgaa atacgaaagg acgagttcat
     5461 agtttttaag aaagaagaac tggacgccat aagaccggaa tcggatgaga ggatcaggct
     5521 tgataaattc gtcagcctgc tctccatcga caccgtatat tttgataagt catatatcct
     5581 tgtccctgac aggagcgaag aagcctacag tcttatgatg acagtaattg cgcaattggg
     5641 aaaagcagga atgggaaagg tcactatgag aacaaaggaa tatcctgttg tcgtgcatga
     5701 atataatggc gctttaatcc tgacaaccct gcgctatgct aacgaggttg tgaatcccgc
     5761 aggtatggaa gagctttcgc aactcaaaaa acccgggaaa aaagagatgg aactggccga
     5821 gaagatcatc aatgacctgt ccggggaatt tgatataaca gaatacaggg atagttacag
     5881 ggaaaagatg gaacagctca tagagatgaa gaggaaaggt gaggtagttg tggcgcaagt
     5941 accaaagaaa gaggaagtaa aggaattgat ggcaaccctc caggagacta ttaaacgcct
     6001 gagcaaatga aacaatataa agcgatgctt gcccggccgg cagatgcccc gttttcttct
     6061 gacgactgga tatttgaagt aaagtgggat ggtataaggg ccatcagtta cgtaaaggag
     6121 gaactcagta tcaggagccg taaccagaag gaactgatag ctaatttccc ggaactttct
     6181 gaactgaaag acctggccag ggatatggta ctggacgggg agataattgt catgaaggac
     6241 ggaaaggctg attttgcgac ccttatcaag aggagccaaa atacgaaagc catggatatc
     6301 gatcacatgg cacgaaagtt cccagccaca tatataatat ttgatatcct ggaaaagaaa
     6361 ggtgaggaac ttctggatat tcctcttata gagagaaaga agatattgga aaatagtttg
     6421 aaagaaggac aattcgttgt cctttccctg tttgttgagg ggtcaggagt tgattattac
     6481 cgggcagccc tggaaaaagg cattgagggc gtaatggcta aaaagaagca aagcccctat
     6541 gaaccgggaa aaaggagcaa ctactggctg aaaataaaac aggtaaagac atgtgattgt
     6601 gttatttttg gctatacaaa aggtgagggg aacagggatg agacctttgg atccttgata
     6661 cttggattgt atgatatgga aagaccggtc tttattggta aagtggggac gggcttttcc
     6721 cgggaaatta tggaaaacct gaagcggttt ctggatgaat ataaagtgga ggaagaaaca
     6781 ttgcagggag tagatatgga tcgggaaata acatggctta gagccggggt tgtgtgtgaa
     6841 gtgggttatc agtccataac agaagaccaa aaactgcgta ttcctgtatt tcgaaacatc
     6901 agggaagata aagatccgtt tgaatgtact attgatcaga tccgtcctg
//
LOCUS       DATGBN010000386         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_381210, whole genome shotgun sequence.
ACCESSION   DATGBN010000386 DATGBN010000000
VERSION     DATGBN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381210"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VKL21_09585"
     CDS             <1..304
                     /locus_tag="VKL21_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMB46060.1"
                     /translation="ACAGRATILLKNKKAITEGITAVVNPDVCVGCEVCISMCPFQAI
                     SMDEQAEKAKVMKALCKGCGTCVAACPTGALEQSHFKNNQILAQVKNVFKFQEVAA"
     gene            301..762
                     /locus_tag="VKL21_09590"
     CDS             301..762
                     /locus_tag="VKL21_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46061.1"
                     /translation="MSKKAILISANPNYGTGGDVNKHKEHADKFGKVFWPLIPPGDRD
                     IKWAHDDIEKGYFYTSGTSKKIQYEFLIDKAGKISDFEFENHKRYIHEDREWLWKKPK
                     CGYRYALLITNINKLTHEISLDKFTLARTNEPVKRVMNYVIVKDKISSRGS"
     gene            764..1243
                     /locus_tag="VKL21_09595"
     CDS             764..1243
                     /locus_tag="VKL21_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46062.1"
                     /translation="MSNKAIIIAANPEINDDLGNVEKHKEHAEQVGKVFWDLVPPGQR
                     EISWKHPEIQKGYFYISGTGKIQYKFLIDKAGTVSEFKKSSLWNDEKKFIPKKIRNEQ
                     WEKSSYDYFFALLIKNIVKIDPISLEEFQLVNTGESAKRVMNYIIVKDDKRTTEVLS"
     gene            1240..1692
                     /locus_tag="VKL21_09600"
     CDS             1240..1692
                     /locus_tag="VKL21_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HMB46063.1"
                     /translation="MSAEAATDAKTDGWEPKIVAFCCNWCSYGGADSAGMGRFQQPAS
                     TRIIRVMCSGRIDPLHVFNAFLEGADAVLVTGCHIGDCHYVNGNDKTKIKYKYLENVA
                     HELGIEKERLQLNWISASEGEKFANFIRDVTAQIKKLGPSPLKPEGVA"
     gene            1692..2843
                     /locus_tag="VKL21_09605"
     CDS             1692..2843
                     /locus_tag="VKL21_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HMB46064.1"
                     /translation="MQKGEMYVGWSTKDDVRKRGGSGGLVTSMLAAALEKKLVDAVVV
                     LKKINEFEAVPIITSDINEVLNSAGSLHSVPSHFAKLIANKNLKVALPAKGCDVRGII
                     EQGKRNAINLENTFIVGLNCGGSMHPVVTREMLEVMYKIKPEDVHGEEIEKGKLIFKT
                     GDGKENAITIDELEEKGYGRRESCRYCTIKIPNNSDIACGNWGVTGDLVGKATFVEIN
                     SEKGAMLLQNAVDAGYIQVQKPDDKGIAMRAKIKGVMEGLGQKWKDRIFLPMDNRLEY
                     FRKELEHCIDCGACKTVCPTCSCGDVSKCTEYHSRSDAYKMSMYHLVRFLHLADACIG
                     CGQCSDVCPVDIPLTRLYRRFANPIQQELKYEPGMDMRKPPYFEVKLNE"
     gene            2836..3210
                     /locus_tag="VKL21_09610"
     CDS             2836..3210
                     /locus_tag="VKL21_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46065.1"
                     /translation="MSEDFSFNRKQKKEEESGFSSDICDSLRKGTLAKENIETLGNSS
                     GLTPLNFCVDECNRILNRGHVSDGLSTVRPFAQINQRGLDSDPLNKGLSQSTEIQRCL
                     SGLCSPLGLSVEIQKRRNLNER"
     gene            3200..>4306
                     /locus_tag="VKL21_09615"
     CDS             3200..>4306
                     /locus_tag="VKL21_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46066.1"
                     /translation="MSDNVYSTICPGCNFGCGLYIRENGSMEVDFRKSSPANAGKLCR
                     FGMSLPKFFTPIKSMIDGNETTMEEAAKEASKRISASKGSVAFLSLGNTANEELLAFL
                     KIAESYLCTGVNFEALPKDAYQTMSIGIPYSEIEAAKHIVLFIDPYEQYPLIVRRLLL
                     AKNKGAKITAVWWKDLTLADENIRPDDFSKLTLTKDSIIIADFHPLSDPEQVKNVLSL
                     AKNAGAKVTFLKPFANSTGEYIISKDAKQKSLAQLIDDINKGTIKTLFALESDLSALI
                     PSDTLSKLTNLIIQAGQASSAKANIVIAHEPLYKKKGTLINNEGRVQTLGSAGTNGLD
                     ALGIIAGSKFDFNTLHESIKKTIGITVVDEFAIPK"
BASE COUNT         1387 a          809 c         1045 g         1065 t
ORIGIN      
        1 tgcttgtgca ggacgcgcaa ctatcttgct caagaacaag aaagcaataa cagaaggcat
       61 tacagcagtt gtgaaccctg atgtatgtgt aggatgcgaa gtatgcatat ccatgtgccc
      121 cttccaggcg atcagtatgg acgaacaggc agaaaaagca aaggtaatga aagcgctatg
      181 taaaggctgc ggaacatgcg ttgctgcatg cccgactggc gctcttgagc aaagccactt
      241 taagaataac cagatacttg cccaggtcaa gaatgtgttc aagtttcagg aggtagcggc
      301 atgagcaaga aagctattct tatttcagct aatccaaatt acggaacagg tggagatgtc
      361 aataaacata aggaacatgc cgataaattc ggtaaagttt tctggccttt aattcctcct
      421 ggggatcggg acataaaatg ggcacatgat gatattgaaa aggggtattt ttatacttca
      481 ggtacctcaa aaaaaattca atatgaattt cttattgata aagctggtaa aatttctgat
      541 ttcgaatttg aaaatcataa gagatacatt catgaagata gggaatggtt atggaagaaa
      601 ccgaaatgtg gatatcgtta tgcactctta ataacgaata ttaacaaatt gactcacgaa
      661 atctctcttg ataaatttac cttagcaaga actaatgagc cagtcaagag agttatgaat
      721 tatgttatag ttaaagataa aatatcatcg agggggtcgt agaatgagca ataaggcaat
      781 aataatagct gcaaatccgg aaataaacga tgaccttggt aatgttgaaa agcataaaga
      841 acatgctgaa caagttggaa aagtattttg ggatttagtt cctcccggac aacgtgagat
      901 ttcctggaaa cacccagaaa ttcaaaaagg atatttttat atatcgggga caggaaaaat
      961 tcaatacaaa tttttaatcg ataaagctgg aacggtttca gaatttaaaa aatcaagttt
     1021 atggaatgat gaaaagaagt tcattccaaa aaaaatccga aatgaacaat gggaaaaatc
     1081 tagctatgac tacttctttg cactcttaat aaagaatatt gtaaaaatag atccaatttc
     1141 gcttgaagag tttcaattag taaatacagg tgaatctgct aagagagtta tgaactatat
     1201 catagttaaa gatgataagc gaactacaga ggtgttatca tgagtgcaga ggctgccaca
     1261 gatgcgaaga ccgatggctg ggaacccaag atagtagcgt tttgctgcaa ctggtgttca
     1321 tatggaggcg ccgacagcgc tggcatggga agattccagc agcctgcttc aaccaggatc
     1381 atccgtgtga tgtgttcggg aagaatagac ccgctgcatg tgttcaatgc attcctggag
     1441 ggcgctgacg ctgtacttgt gacaggatgc catattggcg attgccatta tgttaatggg
     1501 aacgataaaa caaagatcaa atacaaatac cttgaaaacg tcgcacatga acttggtatt
     1561 gaaaaagaga gacttcaact caactggata tcggcaagtg aaggcgagaa gttcgcaaat
     1621 ttcatccgcg acgtgaccgc ccagataaag aagctgggtc cgagtcccct gaaacctgag
     1681 ggggtggcgt aatgcaaaaa ggcgagatgt atgttggctg gtcaacaaaa gatgatgtca
     1741 ggaaacgcgg aggctcagga ggccttgtaa cctccatgct ggcggcagcc cttgaaaaga
     1801 agcttgttga tgcggttgtc gtgctaaaaa agataaacga attcgaagcg gtccctatca
     1861 taacttccga tattaatgag gtactgaatt cagcaggttc actgcactca gtcccgagcc
     1921 attttgcaaa gttgatagct aacaagaacc ttaaagtcgc actccccgca aaaggatgtg
     1981 acgtgcgggg gatcatcgag cagggtaaac gcaatgctat aaaccttgag aatacattca
     2041 tcgtgggtct aaattgcggc ggatcgatgc atcctgtcgt tacgcgtgag atgcttgaag
     2101 tgatgtataa gataaaaccg gaagatgtcc atggtgaaga gatcgagaaa ggtaagctca
     2161 ttttcaagac cggggatgga aaagagaacg ccattaccat cgatgaactt gaagaaaaag
     2221 ggtatggtcg gcgtgaaagc tgccgatact gcaccataaa gatacccaat aattccgata
     2281 ttgcctgcgg taactggggg gttaccggag acctggtggg caaagccaca tttgtggaga
     2341 taaactccga aaaaggcgcc atgctccttc agaatgccgt tgatgcaggt tatatccagg
     2401 tacagaaacc tgatgacaag ggaattgcaa tgcgagcaaa gataaaaggc gtgatggagg
     2461 gccttggtca gaaatggaaa gacaggatat tccttcccat ggataacagg cttgagtatt
     2521 tcaggaagga acttgagcat tgtatcgact gcggcgcatg caagacagtt tgtcctacat
     2581 gttcatgcgg cgatgtttca aagtgtacgg aataccattc tcgcagcgat gcttataaga
     2641 tgtccatgta tcacctcgtg cgcttcctgc accttgcaga cgcatgtatc gggtgtgggc
     2701 agtgcagtga tgtatgccct gtggatattc cgcttacaag attatacagg aggtttgcaa
     2761 atccaattca acaggagctg aagtatgagc ctggtatgga tatgcgaaaa cccccctact
     2821 ttgaggtgaa gttaaatgag tgaggatttc agttttaaca ggaagcagaa aaaggaagaa
     2881 gaaagtggat tttcttctga tatctgcgat tcattgagaa aagggacttt agcaaaagag
     2941 aacatagaaa cgttgggaaa ttcttcagga ttaactcctc tgaatttctg tgttgatgaa
     3001 tgcaacagga tcttgaacag aggtcatgta agtgatggat tatcaacagt tcgtccattt
     3061 gcgcaaatta accaaagggg tctggatagt gatcctttga ataaaggatt atcacagagc
     3121 acagagatac aaagatgttt atcaggactc tgttcaccac tcggtcttag tgtggagatc
     3181 cagaaaagga ggaacttgaa tgagcgataa cgtttattca acaatatgcc ccggctgtaa
     3241 ttttggctgc ggtctataca tccgtgagaa tggctccatg gaagttgatt tcaggaaatc
     3301 gtcacctgct aatgccggaa aactgtgcag gttcgggatg agccttccaa agttctttac
     3361 gcccataaaa tcgatgatag atggaaatga gactactatg gaagaggcag caaaggaagc
     3421 gtcaaagaga atttcagcca gtaagggttc tgttgcgttt ctatctctgg gaaatactgc
     3481 aaacgaggag cttcttgcat tcctgaagat tgctgaatca tatttatgca ccggggtaaa
     3541 ttttgaggcg ctgccgaagg atgcatatca aacgatgagc atcggaattc catacagcga
     3601 gatcgaagct gcaaaacata tcgtgctttt cattgacccc tatgagcagt atcctctcat
     3661 cgtacggcgc ttgcttttgg caaaaaacaa aggcgcaaag atcactgcag tctggtggaa
     3721 agatcttacg ctggcagatg aaaacatcag acctgatgat ttctccaaac ttacacttac
     3781 gaaagattcc atcatcatag ccgatttcca tccgctttct gatccggagc aggtaaaaaa
     3841 cgtgctttca cttgcaaaga acgcaggggc aaaagtaacg tttctcaagc catttgcgaa
     3901 ttcaacaggt gagtatatca tttcaaaaga tgcaaagcaa aagtcacttg cgcagcttat
     3961 tgatgatatt aataaaggaa cgataaagac gctttttgct cttgaatcag atctatcggc
     4021 tttgatacca tccgataccc tttcaaaact cacgaacctg ataatccagg ccgggcaggc
     4081 gtcatcggca aaagcaaata tcgtgatagc acacgagccg ctttataaga aaaaaggcac
     4141 gctgataaat aacgagggga gagtgcagac gcttggcagc gccgggacga acgggcttga
     4201 cgcgcttggg ataattgcgg ggagcaaatt tgatttcaat acactccatg aatcaataaa
     4261 gaaaacgatc ggcattacgg tggtcgatga atttgcgatc ccgaaa
//
LOCUS       DATGBN010000387         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_148122, whole genome shotgun sequence.
ACCESSION   DATGBN010000387 DATGBN010000000
VERSION     DATGBN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148122"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..708
                     /locus_tag="VKL21_09620"
     CDS             295..708
                     /locus_tag="VKL21_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46067.1"
                     /translation="MVMKKCCGVHALRIAVRMIVLGLLLAGSANAANLTVCPGGCEYS
                     SIQKAIKSSSNGDTILVKSGTYFEKVKINKKLTLRGIGNPVVDAGESGSAITLAAKGI
                     RLEGFTATGSDHSPESGIEVFSSNNTLSRNIATMF"
     gene            705..1052
                     /locus_tag="VKL21_09625"
     CDS             705..1052
                     /locus_tag="VKL21_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMB46068.1"
                     /translation="MNNTNGIYLSSSSNNTIYNNIFNNNNNFDFQENNDNTWNTKKQS
                     GTNIIGGPILGGNFWGYPNGTGFSQTCKDMNGNGICEKRYILDENNIDYLPLYQDFIP
                     LWRVDVTLAIERF"
     gene            1077..2018
                     /locus_tag="VKL21_09630"
     CDS             1077..2018
                     /locus_tag="VKL21_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMB46069.1"
                     /translation="MFETKKINIGHLSTLYHTSFILMGTDWLKKSGIQANWKLFASGP
                     DIVKAFQKKEIDIGYIGLPPAIIGMDRGVPLVCVAGGHVEGTVLIARKNYKTYHEINV
                     INILMKQFEGKFIGSPPKGSIHDVIINNIINENDLDIKVKNYPWTDFVLEALVDGDID
                     AAVGTPSLAVAALNACGAKIIIPPHMLWPDNPSYGIVIHKDLLHYPEVILKFLKQHEK
                     ASNFIRNYPVEASRLVSRLTGIVDEEFLLDAYRISPKYCAALSKEFVASTIAFVPVLH
                     RLKYISNFISEKEIFEYCFIEKIHKEQPHYNLHNVSL"
     gene            complement(2000..2905)
                     /gene="moaA"
                     /locus_tag="VKL21_09635"
     CDS             complement(2000..2905)
                     /gene="moaA"
                     /locus_tag="VKL21_09635"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMB46070.1"
                     /translation="MTPAMQLTDTYGRTIKSLRISITNRCNLKCIYCHREGETRHTNN
                     EMSVDTIANIVIASKKFGVNKVKFSGGEPLMRNDLEKIIKALPELKDISATTNGTSLS
                     QRAHDLAWSGLNRINISLPSLSPERYRKVTGGDVNIVFDGIDAAVASGLIPVKLNMVL
                     MKGLNTDEIPAMMDFIKKYDGKVILQLIELMNFQQTTRYQVNINEVEKFLEKRADSIE
                     ERSMHRRRKYHIDGIEVELVRPIDNSHFCANCNRLRVTSDGKLKPCLLRNDNLVDVNN
                     KEPGEIEELMKLAMEKREPFYKDTL"
     gene            complement(3052..>3507)
                     /locus_tag="VKL21_09640"
     CDS             complement(3052..>3507)
                     /locus_tag="VKL21_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46071.1"
                     /translation="AYTIGRFSIEMNRDDVSVTAIDTLKRIGEMAIEKKFDNKEVPRF
                     LGNLGGYASRKSDEKIALSSIVALETISLNVDKDLRNSFKEIARSLIRIGTVALREKL
                     DKIALSAAQNLTNMSGKDERTIAIVKTLLDESVEGIQDFKKMYLKIQVK"
BASE COUNT         1039 a          703 c          652 g         1113 t
ORIGIN      
        1 ggctcaagtg aggtatcata tatcgctgta atgttttttg catcatcagt cagatcaagc
       61 ccgttctgta tctgttttgc gaacatagaa ttacaattaa aatgaagagc aataaataac
      121 tattgtcttt tcaagactaa aaccaggaag acatctttga atgatgggtc tgatttgcgc
      181 tgagtgccat tctacatctt tcagacatat catgtcaagg acaggttcat aacagtgaaa
      241 cgtaatctgc ctattttata tacatgatga cttctccaat acataggtgg aaacatggtc
      301 atgaaaaaat gttgtggtgt gcatgccctc cgaatagcgg tgagaatgat tgttttggga
      361 ttattgcttg caggcagcgc aaatgcagcg aaccttactg tttgccccgg cggatgtgaa
      421 tactcaagca tacagaaagc gataaagtct tcaagtaatg gggatactat tttagtcaaa
      481 agcggtactt atttcgagaa agtgaagata aacaagaagt tgacccttcg aggtattggc
      541 aatcctgtag tggacgccgg ggaaagcgga agtgcgatca cgcttgctgc aaaaggaatt
      601 agacttgaag gatttacagc gactggttca gaccattctc cagaatccgg aatcgaagtt
      661 ttttccagca acaatacact gagcaggaac attgcaacaa tgttttgaat aataccaacg
      721 gcatctatct gtcctcttcc agcaacaaca ctatatataa caacattttc aacaacaaca
      781 acaattttga ttttcaagaa aacaatgata acacatggaa caccaaaaaa caatcaggca
      841 ctaacatcat cggaggccca atccttggcg gtaatttctg gggatatcct aacggtacag
      901 gattcagcca gacatgcaag gatatgaatg gtaatggaat atgcgaaaaa agatatattc
      961 ttgatgaaaa taatatcgat tacctgcctc tttatcaaga ctttatccca ctttggagag
     1021 ttgacgtgac actagctata gaaaggtttt aatcactgct tctccttaaa aataacatgt
     1081 ttgaaacaaa aaagatcaat ataggccatc tttctacttt atatcacacc tcttttattc
     1141 taatgggtac ggattggttg aagaagtccg ggatacaggc gaactggaaa ctttttgctt
     1201 caggacctga tattgtaaaa gctttccaaa aaaaggaaat tgatataggt tatattggtc
     1261 ttcctcctgc aattatcggt atggatcgcg gcgttcctct ggtatgcgtg gccggtggac
     1321 atgttgaagg aacagtgttg atagcccgga aaaattacaa aacttatcat gaaattaatg
     1381 ttatcaacat cctcatgaag caatttgaag ggaaattcat aggctctccc ccaaaaggtt
     1441 ccatccatga tgtcattatc aataatatca taaatgaaaa tgaccttgat ataaaagtaa
     1501 agaactatcc atggactgac tttgttctcg aagcgctggt agatggagat attgatgctg
     1561 cggtaggaac tccatctctt gctgtggcag cattgaatgc ctgcggtgca aaaataatca
     1621 ttccacccca catgctctgg ccggataatc caagctacgg gatagttatc cacaaagact
     1681 tactacatta ccctgaagta atcctgaaat tccttaaaca gcatgaaaaa gcaagtaatt
     1741 tcatcaggaa ttacccggta gaagcttcca ggcttgtttc aagacttaca gggatagttg
     1801 atgaagagtt ccttcttgat gcctaccgga tttccccaaa atattgcgcc gccctgtcaa
     1861 aagagtttgt ggcatccaca attgcttttg taccggtact tcacaggtta aaatatatct
     1921 caaatttcat atccgaaaaa gaaatattcg agtattgttt tatagagaaa atccataaag
     1981 aacagccgca ttataacctt cataatgtat ctttatagaa gggttctctt ttttccatgg
     2041 ccagtttcat taattcctcg atttctcctg gctctttatt attgacatcc acaagattat
     2101 cattccttaa aagacagggc ttcagctttc catcagaagt caccctgagc ctgttgcaat
     2161 ttgcgcagaa atgcgaattg tctatggggc ggacaagttc aacctcaatg ccatctatat
     2221 gatacttcct tctcctgtgc attgatcgct cttcaatgct atctgccctt ttttcaagga
     2281 atttttccac ttcattgata tttacctgat atcgggttgt ctgctggaaa ttcatcagtt
     2341 caattagttg gaggattacc tttccatcat acttctttat aaaatccatc attgccggaa
     2401 tttcatctgt gtttaatcct ttcatgagga ccatattcaa tttgaccggt atcagaccgg
     2461 atgctactgc ggcgtctatt ccatcaaata cgatattcac gtcaccacca gtcactttcc
     2521 tgtatctttc aggagatagg gatgggagac ttatgtttat cctgttaagc ccgctccagg
     2581 caagatcatg tgctctttgt gaaagagatg tcccgttcgt tgtagcagaa atatccttta
     2641 attccggaag agctttgatg attttttcta gatcgtttcg catcaaaggt tcacccccgg
     2701 agaatttcac tttattgacc ccgaacttct ttgaagctat cacgatattg gctatggtat
     2761 ctacactcat ctcattattt gtatgccgtg tctccccttc gcggtggcaa taaatacatt
     2821 tcaggttgca gcgatttgtg atagaaattc gcaggctctt tatggttctt ccataagtat
     2881 ctgttaattg catagcaggt gtcatgattg gaataatgtg tgctttctcc tatattctag
     2941 ccaagattaa atattgtgca aaaattttcc gattttatcg gaatggcagg aatacttgag
     3001 gggggatatc gagacttgga gttttaagat caaaatagcc tacctataaa tttattttac
     3061 ttgtattttt aaatacattt ttttaaagtc ttgaattcct tcaacagatt catctaaaag
     3121 agtcttaaca attgcaatag ttctttcatc cttacctgac atgtttgtga ggttttgtgc
     3181 tgcagataag gctattttat ctaatttttc acgtagtgca accgtaccga ttcttattaa
     3241 agaccgagcg atttctttaa atgaatttct caggtcttta tcaacattca ggcttatggt
     3301 ttctaaagcc actatcgatg acaatgcaat tttctcatcg ctttttctcg atgcataacc
     3361 tccaagatta cctaaaaatc ttggtacttc tttattatca aatttcttct ctattgccat
     3421 ttctcctatt ctttttaacg tatctatagc tgttacagac acatcatctc gattcatttc
     3481 aatagaaaat ctacctatgg tgtatgc
//
LOCUS       DATGBN010000388         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_225731, whole genome shotgun sequence.
ACCESSION   DATGBN010000388 DATGBN010000000
VERSION     DATGBN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225731"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="artA"
                     /locus_tag="VKL21_09645"
     CDS             <1..563
                     /gene="artA"
                     /locus_tag="VKL21_09645"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HMB46072.1"
                     /translation="YEVFISLSRAASISGLIYFLFAEVELLNRWIISTVTEEGIWLIG
                     KFDISVTNVAWNQLAVNGLVVEIILACTAIESIALFSGMISSATGATAARKVQAFMIS
                     VPVVYLLNIIRVSFTASAFGLSWFGTPEESFHISEHFITKAGSLLALMLISYYVLKML
                     PEVSDMIDGVLKMMRTEFQRLILRLS"
     gene            560..847
                     /locus_tag="VKL21_09650"
     CDS             560..847
                     /locus_tag="VKL21_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HMB46073.1"
                     /translation="MKIKVNYDFTHFELVGDTDIFEVADGVKLGDLLRLIDARIMEVG
                     RNKGIETTYKTTMAGDQLNACMVFINGSAPGLDHELHDGDTIEFVYGFCGG"
     gene            864..>1594
                     /locus_tag="VKL21_09655"
     CDS             864..>1594
                     /locus_tag="VKL21_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HMB46074.1"
                     /translation="MTDFINRNYIEGIISDTSYNHFDMITYVIILFAGVYGVLKLLNK
                     LRIKVDEEFVIATIPYIFMGSVFRVIEDADLLKPPVKYFFITPLIFFVIFAICFGTLL
                     LTRYLEKIKKIRNYIRIYATVGVVLSLAGIAILIYYNSPGWNPIILVYSLVPAIILTY
                     IVRRASPAIGMTYLRSRVYSFAVFSFMLDSFTTYIGVDLFGYINKHPFSSFLTSIFGT
                     GAVLIPLSLILVLVIVFFLEKESRE"
BASE COUNT          433 a          261 c          354 g          546 t
ORIGIN      
        1 actatgaagt gtttatttcc ttatccaggg cagcatctat tagcggtttg atatatttcc
       61 tgtttgcaga ggtagaatta ttaaatagat ggataatatc aacggtcaca gaagagggga
      121 tatggttgat cggaaaattc gatatttctg ttacgaatgt agcatggaac cagcttgcag
      181 taaatggcct ggtagttgaa ataatacttg cctgcactgc gatcgagagc attgccctgt
      241 tttcaggcat gatttcttct gctacgggtg cgactgctgc gcgcaaagtc caggcgttta
      301 tgatctcagt gcctgtagta tatttgctga atattatccg tgtctctttt actgcctcag
      361 cctttggatt aagctggttt ggcacacctg aagaaagttt tcatatttca gagcatttta
      421 ttacaaaggc aggctcgttg ctggctctaa tgttgatttc ttattacgtt ctaaaaatgc
      481 tgcctgaggt ttccgatatg atagatggtg tcctgaagat gatgagaaca gaatttcaga
      541 gattgatatt aaggttatca tgaaaataaa agtaaattat gatttcactc actttgaatt
      601 ggttggggat acagatattt ttgaagttgc agatggagtc aaacttggag atttgttgcg
      661 gttaatagat gccaggatca tggaagtggg aagaaacaag ggaatagaga ctacttataa
      721 aacaaccatg gctggggatc agttgaatgc ttgcatggtt tttattaatg gttcagcgcc
      781 agggctggat catgagttac atgatgggga tactattgaa tttgtatatg gattctgcgg
      841 cggatgagtg atttaagttg gatttgaccg atttcataaa caggaattat attgaaggta
      901 tcatcagtga taccagttat aatcattttg acatgatcac atatgtcatt attttatttg
      961 ctggtgtata tggagtttta aaattgctga ataaattgag gatcaaagtc gatgaggagt
     1021 ttgttatagc tacaatccct tatattttca tgggctccgt gttcagggta atcgaggatg
     1081 cagatcttct gaagccgcct gtcaaatatt ttttcattac accgcttatt ttctttgtga
     1141 tctttgcaat atgttttgga acacttctcc tcacaagata tcttgagaag atcaagaaaa
     1201 taaggaacta cattcgcatt tatgcaactg tgggtgtcgt attatcgtta gccggaattg
     1261 caatcctcat ttactataat tctcctggct ggaatcctat tatcctggtg tacagtcttg
     1321 tgcctgcaat aatattgacg tatatcgtca gaagagcctc acctgctatc ggtatgactt
     1381 atcttcgaag cagggtgtat tcatttgcgg tcttttcatt tatgctggat tcgtttacaa
     1441 catatattgg tgtagatcta tttggctaca taaacaaaca tcctttcagt tcgttcctga
     1501 cgtccatttt tggaacagga gctgtactga tccctttgtc tttgatctta gtccttgtaa
     1561 tagttttctt ccttgaaaaa gaatccaggg aaga
//
LOCUS       DATGBN010000389         4112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_225785, whole genome shotgun sequence.
ACCESSION   DATGBN010000389 DATGBN010000000
VERSION     DATGBN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225785"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VKL21_09660"
     CDS             complement(<1..221)
                     /locus_tag="VKL21_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46075.1"
                     /translation="MELDVGWLMIIIGLVLLIIEASQPGFFVAVPGTILIVLGIVTLF
                     IPEVAQDYAPAIIIVTALVSSIITIVFYR"
     gene            352..1263
                     /gene="pyrB"
                     /locus_tag="VKL21_09665"
     CDS             352..1263
                     /gene="pyrB"
                     /locus_tag="VKL21_09665"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HMB46076.1"
                     /translation="MLRHIISMKDFSRDEIDIILNNTMDFEPKARGQTSSLLSGKVLA
                     TLFYEPSTRTRLSFETAMKRLGGEVIDLGPIEKSSVAKGETLADTIRVIGNYADAIVL
                     RHPREGAARMAAEYSNVPIINAGDGAGHHPTQTLLDLYTIMRESRLSDLRIALVGDLK
                     YGRTVHSLAYALSMYHADMTLISPKQLQMPDVIKKDLKKQGARIRETTNIEEVIGDID
                     VLYVTRIQKERFPDPAEYQKVAGIYRVTEELLKKARDNLIIMHPLPRVDEIDSAVDRT
                     KYARYFEQSFYGVPVRMALLAMAMGVV"
     gene            1260..1751
                     /gene="pyrI"
                     /locus_tag="VKL21_09670"
     CDS             1260..1751
                     /gene="pyrI"
                     /locus_tag="VKL21_09670"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HMB46077.1"
                     /translation="MNQKKNTSDGSLNKEMRVRPIMQGTVIDHITAGQALNVLKILGI
                     SGTTTAVVSVAMNVPSEVMGNKDIVKIEDRELEEQEVDRVSLIAPNATINIIRDFEVV
                     EKYRVDLPDRLEGVMKCDNPNCISNTSEPVISKFTVNKKPVELRCIYCDHVISQGITE
                     HLI"
     gene            1792..2049
                     /locus_tag="VKL21_09675"
     CDS             1792..2049
                     /locus_tag="VKL21_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46078.1"
                     /translation="MDENTINRTKAAINALIDIEQLWIENTPNYNLSTQELLVLKKRL
                     ERASENVSRIYEDNKVRLQAAEEEIKKMHVGKKESKNNKRK"
     gene            2134..2328
                     /locus_tag="VKL21_09680"
     CDS             2134..2328
                     /locus_tag="VKL21_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HMB46079.1"
                     /translation="MNDGIYRDKIKVGLNVGIVLKQDQRNGKITHGIVKRVLTNSSCH
                     PHGIKVELEDGHVGRVKEIH"
     gene            2361..3605
                     /locus_tag="VKL21_09685"
     CDS             2361..3605
                     /locus_tag="VKL21_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HMB46080.1"
                     /translation="MPTISEKILSCASGKTVQADDFVIANVDYAMAHDGTSVLAVKAF
                     KEMGEKKVWDPGRIIIPFDHIAPANNETAAKLQKSIRDWIKEQGIVNFFDVGEGICHQ
                     VVPEQGFAMPGKLMIGADSHSCTYGAFGAFATGVGATDMAQIFATGKLWLRVPQTIRI
                     TVEGKLAKGVSAKDMILFIIKNVGADGANYKAVEYYGSTISELSMSQRMTLCNMSIEM
                     GAKTGIVPSDDKTFEYLKGRAREKFVPVYADKDAKFCQEFNFDVGELEPQIAKPHEVD
                     NVTGIGTLLGTPLDQVFIGSCTNGRFEDLEVVANILSGKKVKIRTIVLPASRSVLIKA
                     TESGIIATLLKAGAIIAPPGCGPCLGAHMGVICEGETCLSTSNRNFKGRMGSGGLIYL
                     SSPETAAASALKGEISDPREHY"
     gene            3687..>4112
                     /locus_tag="VKL21_09690"
     CDS             3687..>4112
                     /locus_tag="VKL21_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMB46081.1"
                     /translation="MTEKADNASTSTSPTPVINPPVMNGSYHDLKNRLAEKMAGEITL
                     SDNPGETLKKWRASFEIAQSDLASFLKVSPSVISDYESGRRKSPGIFIVSKIVEAILE
                     MDATRGGAKTRSYEGMLRGGFSADAIYEIHEYLSPLSIEE"
BASE COUNT         1306 a          800 c          989 g         1017 t
ORIGIN      
        1 ttccgataaa agacgattgt gataatactt gaaaccagcg ctgtcactat aattatcgca
       61 ggcgcataat cctgcgccac ttcgggaata aaaagggtga caatgcccag gacaataagt
      121 atcgttcccg ggacagccac aaaaaaacct ggctgtgatg cctctatgat aagaagcact
      181 aaaccgataa ttatcattaa ccaaccaaca tccaattcca tagactttaa gatagttgtt
      241 attagtatat atcatatggg gttggtagag aagtcttctg aaagggtggg gtagttcacc
      301 cattatccca ttatcaataa atataaattt caatgccgca tattagagtt gatgttacga
      361 catatcatct caatgaaaga tttttcgcgt gatgagattg atattatcct gaataataca
      421 atggattttg aaccaaaagc acgggggcaa acatcctccc ttctttccgg taaggttctg
      481 gcaacacttt tttatgagcc gagcactcgc acgcgcttat cttttgaaac ggcgatgaaa
      541 aggcttggag gggaggtaat tgacctgggt ccgattgaaa aaagctcggt cgcaaaagga
      601 gaaacacttg ctgacaccat cagggtaata ggaaattatg cagatgctat cgtattgagg
      661 catcccaggg aaggcgcggc gcgaatggct gcagaatatt caaatgtccc tataattaat
      721 gcgggagacg gggcaggaca ccatcccacg cagacgcttc ttgaccttta tacgataatg
      781 cgtgagagca gactctcaga tctgaggatc gcacttgttg gggatctcaa gtacggaagg
      841 acggtccatt cacttgctta tgctttatcg atgtatcatg ccgacatgac actgatctcg
      901 ccaaagcagc tgcaaatgcc tgatgttatc aagaaagacc tgaaaaaaca gggtgcaaga
      961 atcagagaaa ccacaaatat cgaggaagta ataggagata tcgatgtatt atatgtgaca
     1021 aggatccaga aagagcgctt ccccgaccct gctgaatacc agaaagtagc aggcatatac
     1081 agggttacgg aggaactgct aaaaaaggcg cgcgataatc tcataattat gcatccgctt
     1141 ccgcgtgtgg atgagatcga ttccgctgtt gacaggacaa aatatgcgcg gtattttgag
     1201 cagtcatttt acggggtccc tgtcaggatg gcacttcttg caatggcaat gggggttgta
     1261 tgaaccagaa aaaaaatact tctgatggtt cattgaataa agaaatgaga gtgcgcccta
     1321 tcatgcaagg tacagttatc gaccatatta cggcaggcca ggcgctcaat gtactgaaga
     1381 tcctggggat atcagggact acaactgctg tggtcagcgt cgcaatgaac gtgccaagtg
     1441 aagtcatggg aaacaaggat atagttaaga tcgaggatag ggagcttgaa gagcaagagg
     1501 tggacagggt ttcccttata gcgccaaatg ccactataaa tatcattcgc gattttgaag
     1561 tcgtcgagaa atacagggtt gaccttcccg atcgtcttga gggtgtgatg aaatgcgata
     1621 accccaactg catttccaat actagcgaac ctgttatttc aaaattcacg gtaaataaga
     1681 agccagtgga acttcggtgc atttactgcg atcatgtgat ttcccagggt ataaccgaac
     1741 atttgatttg agatgcaaac tttaatctaa gaaaaagttc aatttcacca catggacgag
     1801 aacaccataa acaggacaaa ggccgcaatt aacgctctca tagatataga gcagttgtgg
     1861 atcgagaaca ctcctaatta taatctttct acacaggaac tgcttgtcct gaaaaaaagg
     1921 cttgagcggg cttctgagaa tgtttccagg atatatgagg ataataaggt aagattacag
     1981 gccgctgaag aagagatcaa gaagatgcat gtcggaaaaa aagaaagtaa gaataataaa
     2041 agaaagtaag aatattaaaa gaaagtaaga atattaaaag aaagcaagaa tattaaaata
     2101 aataaatatt aaaataagaa tattgataat attatgaatg atggtattta tcgggataaa
     2161 attaaagtcg gattaaatgt cgggatagtt ttaaaacagg atcaaaggaa tggaaaaatt
     2221 actcatggca ttgtaaagag agttttgaca aattcttcat gccacccgca tggaataaaa
     2281 gttgaattgg aagatggaca tgtgggaaga gtgaaggaaa ttcattagtt atttatttct
     2341 taagatccta atacctgcaa atgcctacca taagtgaaaa gatactctcc tgcgcctcag
     2401 gaaaaacagt tcaggctgac gattttgtga tcgcgaatgt tgattatgcg atggcgcatg
     2461 acgggacaag tgtgcttgct gtgaaagcct tcaaggaaat gggagaaaag aaagtatggg
     2521 atccagggcg gattattatt ccatttgacc atatcgcgcc ggcaaataat gaaaccgccg
     2581 caaagctcca gaagagtatc agggattgga taaaagagca ggggattgta aatttcttcg
     2641 atgtgggaga aggcatctgt caccaggtcg tacctgagca gggatttgca atgccgggaa
     2701 aactcatgat cggggcagat tcgcattcat gcacctatgg cgccttcgga gcatttgcca
     2761 caggggtcgg agctactgat atggcgcaga ttttcgcaac aggcaagttg tggctgcgcg
     2821 tcccgcaaac cataaggatc actgtagaag gaaaactcgc aaaaggcgta tcggcaaagg
     2881 atatgatcct tttcataatt aaaaatgtgg gcgcagacgg ggctaattat aaagcggttg
     2941 aatattatgg cagtacgatc agtgaactat caatgtccca gcgcatgact ctttgcaaca
     3001 tgtctatcga aatgggagca aaaacaggta tcgtgccatc tgatgataag acgttcgaat
     3061 acctgaaggg acgggcgcgt gaaaaattcg tgcctgttta tgctgataag gatgcaaaat
     3121 tttgccagga attcaatttt gatgtaggag aacttgaacc acaaattgct aaaccgcatg
     3181 aggtagataa cgtaaccgga ataggaaccc ttttgggcac acctcttgac caggtattca
     3241 taggatcatg cacgaatgga agattcgagg acttagaagt tgtagcaaat atcctgtcag
     3301 gcaagaaagt gaaaattcgg acaattgtgc tcccagcatc gcgttctgtc ctgataaaag
     3361 caactgaatc gggcatcatc gccactcttc tgaaagctgg cgccatcatt gcgccgcccg
     3421 ggtgcggacc gtgcctgggc gcccatatgg gtgtaatatg cgagggcgaa acatgcctgt
     3481 ctacatccaa ccgaaatttc aagggcagga tgggatcggg aggattaata tacctatctt
     3541 ccccggaaac agctgctgca agtgcactca agggagagat ttcagacccg agagaacact
     3601 attgattttt tgagatcgca cttcgttatt tgacgatgtc aggttcgtta attttatatg
     3661 tcataaaatc catacctcgt cctaatatga ccgaaaaagc agataatgct tcgacttcca
     3721 cctctcctac tcctgttatt aatcctcccg taatgaatgg ttcttatcat gacctgaaga
     3781 acagactggc tgaaaaaatg gcaggagaga ttaccctgtc ggataatccg ggagagacat
     3841 taaagaaatg gcgggcaagt tttgagatag cccaatccga cctggcttct tttcttaaag
     3901 tttccccttc cgtaataagc gattatgaaa gcggaaggag aaaatccccg gggattttta
     3961 tcgttagcaa gatagttgaa gccatccttg aaatggatgc tacgcgcggc ggggcgaaaa
     4021 cgcgttctta tgagggaatg ctccgtggcg gtttcagcgc tgacgcaata tacgagattc
     4081 atgaatacct ttccccctta tccatagaag aa
//
LOCUS       DATGBN010000390         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_187282, whole genome shotgun sequence.
ACCESSION   DATGBN010000390 DATGBN010000000
VERSION     DATGBN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187282"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..952
                     /locus_tag="VKL21_09695"
     CDS             176..952
                     /locus_tag="VKL21_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 family protein"
                     /protein_id="HMB46082.1"
                     /translation="MRIYIAYNGKFGERIIGNLLNYRNFCISCDKLCIFCRDDKSLNF
                     AENITGIYTQPCDLPVYIEDPEKFLPSSLPENDVLLAINLHPDIIAEFPALAKKAGTR
                     LIIAPIEEPNWLSAGLRNQIKEKCEKAGIVFAAPKPFCSLKKGEDPLIDQFIETFKIG
                     FPRFKIGIQHNNIVTVQIFTSQPCGCAYYVAQKLKNVPIDSKLDEVISSAHHAYPCTA
                     SMDRDTELKDTILHKAGYIIREAVHEAIFLRNGTCKLKRM"
     gene            931..2007
                     /gene="corA"
                     /locus_tag="VKL21_09700"
     CDS             931..2007
                     /gene="corA"
                     /locus_tag="VKL21_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMB46083.1"
                     /translation="MQIKKDVTIKKRSEKSGLPPGTLLHIGDKKMETTRISLMDYDES
                     HFLEKEVKQVEESFPFKDKPTVTWINIEGIHEIEIIEKIGQYFGVHPLILEDILNTEK
                     RPKMEDFETYIYVTLKMLKQIDNSDKILSEQVSLIIGTNFVISLMESKDDIFDTIKDR
                     IRSGKGHIRKMGSDYLAYSLIDAIVDNYFVIMEKFGEKIESMEEELVKNPTPGTLKSI
                     HDTQRELMILRKSVWPLREVVNKMLRGESTLIHESTLIYLRDVYDHTIEVIDMIEGLR
                     EMVSRMFDIYLSSISNKLNEIMKVLTIIATIFIPLTFIVGVYGMNFEYMPELKWRWGY
                     PVIWTIIVFIGIYMLNYFRKKKWI"
     gene            complement(2019..2843)
                     /gene="ygiD"
                     /locus_tag="VKL21_09705"
     CDS             complement(2019..2843)
                     /gene="ygiD"
                     /locus_tag="VKL21_09705"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HMB46084.1"
                     /translation="MERDISGKNDTVKMPVLFIGHGSPMNLILRNNFTRSLEKLGKNL
                     PKPKAILVISAHWLTGGTYVTCMEKPELIYDFYGFPHELYEMKYASPGSPDDARFVTG
                     TVKKTQVKCNYDWGLDHASWAVLRHMYPDADIPVFEMSLDYSFNDWNPKPLQYHYDIA
                     SELADLRTRGVLIIGSGNIVHNLGLIDFRNIDAHPFEWAVEFDEIVKYNLINKNHQEL
                     VNFREMGNYASLAVPTLDHYLPMIYAIAMQGQDETLKFTYEGFQNGSISMRCFQIG"
BASE COUNT         1008 a          540 c          572 g          928 t
ORIGIN      
        1 tattggcatt ttagagataa acgatgatta tattacgaat tggtttttat gtaatacatt
       61 gctaaagttt atatattgtt gatagcatcc accgctcttg gtttagtttt atataaaagg
      121 agcgaatatt gaagttatgg aaaattatat acaccttaaa aagtaaattt agaatgtgag
      181 aatatatatc gcatataacg gcaaattcgg agaacgcatc atagggaacc tgttaaatta
      241 caggaatttc tgtatttcct gtgataaatt atgcattttt tgccgggacg ataaatcact
      301 taattttgca gaaaatatta ctggcatata cacccagcct tgcgaccttc cagtgtatat
      361 tgaagacccg gagaaatttt tgccgtccag cctgccggaa aacgatgtat tgttagccat
      421 aaaccttcat cctgatatta ttgccgagtt tccagctctt gccaaaaaag caggcacgcg
      481 tttgatcata gctcccattg aagaacccaa ttggttgtct gctggattaa ggaaccagat
      541 caaagagaaa tgcgaaaaag ctggcattgt atttgctgcc ccaaaaccat tctgttctct
      601 taaaaaaggc gaagatccat taatagatca atttattgag acatttaaga tcgggttccc
      661 cagattcaag atcggtatcc agcacaacaa tattgtaacc gtacaaatat tcaccagcca
      721 gccgtgcgga tgtgcttact atgtggcaca gaaacttaag aatgttccta ttgatagcaa
      781 actggatgag gtcatttcaa gcgcacatca tgcatatcca tgcactgcga gtatggatag
      841 agataccgaa ctcaaagata ccatactgca taaagcagga tatattatca gggaagcggt
      901 tcatgaggct atatttctaa ggaatggcac atgcaaatta aaaaggatgt aacaattaaa
      961 aaaagatccg aaaagtcagg tcttcctcct ggaactctac tccatatagg cgataagaaa
     1021 atggagacga caaggatatc attaatggac tatgatgagt cacattttct tgaaaaagaa
     1081 gtaaaacagg tcgaagaatc ttttccattc aaggacaaac caacggtcac atggataaat
     1141 attgaaggga tccatgaaat agagattatt gaaaagatcg gccaatattt tggtgtacat
     1201 cctctcatac ttgaagatat attaaataca gaaaagcgtc caaaaatgga agattttgaa
     1261 acttatatat atgtaacatt aaaaatgctt aaacaaatag acaatagtga taaaatattg
     1321 tctgaacagg tcagtttgat cataggtaca aatttcgtta tttctctcat ggagagtaaa
     1381 gatgatattt ttgataccat caaggacagg atcagaagtg gaaaaggtca tatcaggaag
     1441 atggggtctg attatcttgc ttacagttta atagatgcga tcgtggacaa ttatttcgta
     1501 ataatggaaa aatttgggga gaaaatcgaa tctatggaag aagagcttgt gaaaaatcca
     1561 acaccaggaa ctttgaagtc gattcatgat acccagaggg aattgatgat attgcgaaag
     1621 tcagtctggc ccttgaggga agtagtgaac aaaatgctta ggggagaatc aacccttatc
     1681 catgaatcta cgcttattta tttgagggat gtctacgatc acacgataga ggtcattgat
     1741 atgatcgaag ggttaaggga gatggtttcg agaatgtttg atatttatct ctcaagtatc
     1801 agcaataaat taaatgagat catgaaggta ttaacaatta ttgcaacaat atttattcct
     1861 ttgaccttta ttgttggtgt ttatggaatg aattttgaat atatgcctga acttaaatgg
     1921 cgctggggtt atcctgttat ctggacgatt attgtattca taggtattta tatgctgaat
     1981 tatttcagga agaaaaaatg gatataatcc aaaattgatc aacctatctg gaaacatctc
     2041 attgagatcg aaccattttg aaatccttca taagtgaatt ttagtgtttc atcctgtccc
     2101 tgcattgcga tcgcatagat cattggcaaa taatggtcaa gcgttggcac tgccagggaa
     2161 gcataattcc ccatctccct aaaattgaca agctcttgat gatttttgtt aattaaattg
     2221 tattttacga tctcatcaaa ctcaacagcc cattcaaagg gatgtgcatc aatatttcga
     2281 aaatctatca atcccagatt atgcacaata tttccactgc ctattatcaa aactccgcgt
     2341 gtccttaaat cagcaagctc agaggcaata tcataatgat actgtaatgg ttttggattc
     2401 cagtcattaa aagaataatc aaggctcatt tcaaatacgg gaatatccgc atcagggtac
     2461 atgtgtcgaa ggacagccca tgaagcatgg tctaatcccc aatcataatt gcatttgacc
     2521 tgtgtttttt tcacggttcc tgtcacaaac ctggcatcat ccggtgaacc cggacttgcg
     2581 tatttcattt catataattc atgcggaaat ccataaaaat catagatcag ctccggtttt
     2641 tccatacagg taacgtatgt ccctcctgta agccagtgtg ctgagatgac aagtatcgcc
     2701 tttggctttg gtaaattctt tccaagtttt tccagggatc tggtaaagtt atttctcagg
     2761 atcaggttca taggtgaacc atgacctatg aagagaactg gcattttcac ggtatcgttc
     2821 tttcctgaaa tatctctttc cattttcata agcttaatta tctataggca caatcccaaa
     2881 atccagtgat ttttgacttt ttttcaaaaa atctaacaaa acatcagcaa ttcccgtttt
     2941 atcacacact aatttttttt ggctgtttta atctagattt caccttacat aaagcgtaca
     3001 ttatcaggct gtcccaaaac cccaaattgt aaaaatatta aataagtt
//
LOCUS       DATGBN010000391         5922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_381837, whole genome shotgun sequence.
ACCESSION   DATGBN010000391 DATGBN010000000
VERSION     DATGBN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381837"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..2510)
                     /locus_tag="VKL21_09710"
     CDS             complement(186..2510)
                     /locus_tag="VKL21_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMB46085.1"
                     /translation="MIYFTYPLIAVLIIPISLIILYYLKPKSSGNILRAAIKLIVIFI
                     ILLSIASPYTFIEHEGKTGSDQIIIISDNTPSMLPYDPDIANKVYDYLSNRTQVSIDL
                     VSGTNSPIGDSIFRNIRPGSNILLISDGNNNKGRSITEVVNFARNINSTIYYLRQEPV
                     KNDISVSISGDAMAIIDTPYSFFIDINTLGTIEGDLKIYVDDNIKDTIHVNKRKRIPV
                     EYFFNTQGSHRIKAEISTRGDEISQNNIFYRSVFVIPKPDIQLVSNKESPLSKILDQL
                     YSVDISPSFKNPGSSKAVILDDILSSGLSDNDASILSDYIANGGGLLVVGGPNGYTNY
                     EENPLLEQLLPVKPGGDIVRSKNAAVLLVVDISGSTGDLTGDAAKLGIEKGLANQVIE
                     SRSVDDFIGVIAFNNVPHTIAPLSRYPDNSRVKDTISRLTYGGTTHLSPALSTAFDMV
                     RNFDGGKNVIVISDGQTADSEAALNVAGSMAEEGININTIGVGMDTDEAFMKKLAAKG
                     NGIYLKRDQSDGIRLLFGDIANPAKMDGFPLLLINSDHFITGGVSLNATIYGYNKVFT
                     KQNAQALVMTATGNPVISAWRFGLGRVISITTDNGNTWAPELYKRENSKIISTSINYA
                     IGNPGKSDDIRTQDGETGIPLEIRISSEKEPDLSFDGKPLQFEKTGEEQYMATTIPDS
                     EGFHDISGFTVAVNVPAEYREIGNNELIPGIIGAAGGRVYNTSEIERLIPDIEKKNTA
                     VIRERVELAAFFLFAALLLYGIEVILRRLVEIFR"
     gene            complement(2507..4291)
                     /locus_tag="VKL21_09715"
     CDS             complement(2507..4291)
                     /locus_tag="VKL21_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HMB46086.1"
                     /translation="MLENGPALYLLLILLPVIILYLLKPKPRVIKIPSLLLLHSFSSK
                     RRLRSLFDKLIKDPLLLIQLLAMTILILGIAGPYYFANAEYERTVIVLDTSASMSASD
                     VKPDRFTEALEIAREYPGKSEKNSLILAQNVPVLLYTDENSKQAIDGLKELKPVATGT
                     DLNEAIMFALDLIGNKTCNLVIISDFSGQDISGVQKIIESKNIPVEYRQIGSGGSNLG
                     IVDASIEDNSMGFVVRNYNNIPEDTIIKIMNGDSVKTISRTIKPKSRDFFTVTDMNPG
                     TTKISLEPADDFSLDNMLYISIQYPKNKRILLLRDSSGKSSSISKAFESIPNLEIDKV
                     SFERAPRKLDYGMVVLYDYTRSSLLPGAMEDIKNYATSGGTVVFEAASDLPFLDTKEL
                     LPVNVSGIANPSRIDVKRSGLTNDIDFGVSKYLKGTIKEGAVELASAKEGPILAYRNF
                     GKGKVVYIGTNQDWGDFHLRTSYPIFWYRLLEFADPASGELNFKSGTLLPLDSEKSIK
                     GPHLAIRTDKLYLGETGLYEIEGKTIASNLLDEKESDISIPGINFTKYQEVSGSSMEK
                     IHLYTILSLLAILLIGLELYYLKYRGEI"
     gene            complement(4293..5075)
                     /locus_tag="VKL21_09720"
     CDS             complement(4293..5075)
                     /locus_tag="VKL21_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46087.1"
                     /translation="MYKFIYRVIEIKNILDHLWLIETVLDTAIFVLAGFLLVTYMGIS
                     PYWIIIPIFLNIIVSFRNKSRRNTVTLIEKKYPELNERLRTIYDNKENRNVVLENLAE
                     SVLTDIEKIKYSSFFETKKLGIRIAIILLLVTFLVSSSIINPLYFDQKINEQKINEQK
                     IDPERTPTLTSSSESKNSGGNIFDEPSFVSIANDSQGLLVNRGSGSELNVPGPDRKTP
                     VVYPAFPQNEDTAISSSQAYSEAIPVIYQQIVKNYFMNISRE"
     gene            complement(5077..5598)
                     /locus_tag="VKL21_09725"
     CDS             complement(5077..5598)
                     /locus_tag="VKL21_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46088.1"
                     /translation="MERDVNIKLIYIILILLLALGGTTILYQLRYDNMRDSYEQTFRE
                     YNKTFANMSSDQRSLYSNISDLNVSYERENVLTSKLETKTKELETVSRELAIVQQQLF
                     ENEGKYDAMTLNSSYANEILNRHTTYIGDMQAKIEDLKIHIKTRVEDNTSKDILLNDI
                     SEIQTELDKLKSR"
     gene            5679..>5922
                     /locus_tag="VKL21_09730"
     CDS             5679..>5922
                     /locus_tag="VKL21_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46089.1"
                     /translation="MKACSSCEASVKCGLKDEGLFDLCPKFKTSPDPGKIDMHPDETS
                     PVIDYDILRRYFGYSEFRQLQKDIIQDVLKGNDVLVL"
BASE COUNT         1622 a         1222 c         1147 g         1931 t
ORIGIN      
        1 tcataattat cccgatcccc tgcagccaag ccagggtaac ctcattacta tccacaggca
       61 aaggggaatt tcctgcaata cctgccatac cgacaattgc acaaactgcc attctgaggc
      121 aggtaccagt gaaagatggg ttaaacgaat gaaccatttt aaggtcgttg caaagtgaat
      181 ttaagtcacc tgaatatttc caccagtctc ctcaggataa cctctatacc gtagagcagg
      241 agcgctgcaa agagaaagaa ggcagcaagc tcaactcgtt cccggatcac agcagtattt
      301 tttttttcaa tatccgggat caatctctca atttcagaag tattgtaaac tcgtccgcct
      361 gccgccccga taattcccgg aattaattca ttgttcccga tttccctgta ttctgcgggc
      421 acatttactg ccaccgtaaa tccggaaatg tcatggaaac cttcggaatc ggggattgtt
      481 gttgccatgt attgttcttc tcctgtcttt tcgaattgca ggggcttgcc atcaaaggag
      541 agatcaggtt ccttctctga agaaattctt atctcaagag gtattcctgt ttctccatcc
      601 tgggtcctga tatcatcgct tttcccggga ttgcctatgg catagtttat cgatgttgat
      661 attatttttg aattttccct tttatataat tccggcgccc atgtgtttcc attgtccgtg
      721 gttatggaaa tgacccttcc caggccaaat ctccatgcgc tgatcacagg gttccccgtt
      781 gctgtcataa caagagcctg cgcattttgt tttgtgaaaa ctttattata accgtaaatt
      841 gtcgcattaa gtgaaacgcc tcctgttatg aaatggtcgc tattgatcaa tagaagaggg
      901 aaaccatcca ttttggcagg atttgcgata tcgccgaata ataaccggat cccatcagac
      961 tggtcacgtt tcaagtaaat accgttacct tttgccgcaa gctttttcat aaaagcttca
     1021 tccgtatcca tacccacacc aatagtattg atatttattc cttcttctgc cattgagcct
     1081 gcaacattta atgcagcttc gctatcagca gtttggccgt ctgatattac tataacattt
     1141 tttcccccgt caaaattcct gaccatatca aaagcagtcg atagcgccgg ggaaagatgc
     1201 gtggttccac catatgtgag ccgggagatc gtatctttga ctcttgaatt atcagggtaa
     1261 cgggaaagag gagcgatcgt atgggggaca ttattaaatg caattactcc gataaaatca
     1321 tcaacgctgc gactttcaat aacctggttt gcaagtcctt tttcgatccc gagttttgcc
     1381 gcatccccgg tcagatctcc cgtgcttccc gatatatcaa cgaccaggag cactgctgca
     1441 tttttacttc gaactatatc cccgccgggt tttacaggga gcagttgttc caggagcgga
     1501 ttttcttcat aatttgtata tccattcggc cctccaacaa ctaacagtcc tcctccattt
     1561 gcgatataat cgctaagaat agaagcatca ttgtctgata gtcctgaaga caagatatca
     1621 tccagaatga cagctttact tgatcccgga tttttaaaag aaggggaaat atccacagaa
     1681 tataattgat ccaggatttt tgacaggggt gactctttat ttgatacaag ctggatatcc
     1741 ggctttggaa taacaaatac tgatctgtaa aatatattgt tttgcgaaat ttcatcaccc
     1801 cgggttgaaa tttctgcttt tatcctgtgc gacccctgtg tattgaagaa gtattctaca
     1861 ggaattcgct ttcttttatt aacatgtatt gtatctttaa tattatcgtc aacatagatc
     1921 ttaaggtcgc cttctatagt tcccagggta ttgatatcta tgaagaaact atatggagta
     1981 tcaatgatcg ccatagcatc cccgcttatt gaaacgctta tgtcattttt cacgggttcc
     2041 tgtctgagat aataaatcgt agaattaata tttctggcaa aattgaccac ttcggttatg
     2101 ctccttcctt tattgttatt tccatcgctt atgaggagga tattacttcc aggtctgatg
     2161 ttcctgaaga tagaatcgcc aataggggag tttgttccgg aaacaagatc gatgcttacc
     2221 tgcgttctgt tgctaagata atcatatacc ttgtttgcga tatcggggtc atatgggagc
     2281 atactggggg tattatcgga aattataatg atctggtccg aacctgtttt tccctcatgc
     2341 tctataaaag tgtatggcga tgctattgat aaaagtatta taaaaattac aatgagcttt
     2401 attgccgcac gaagaatatt tcctgagctt tttggtttta gataatataa tataatgaga
     2461 cttataggga ttatgagaac agcaatgagg ggataagtga aatagatcat atttctcctc
     2521 tatacttaag gtaatacaat tcaaggccga ttaacaggat agcaagaaga gataatattg
     2581 tataaagatg tattttttcc atgctgctgc cagatacttc ctggtatttc gtaaaattga
     2641 tccccggaat ggaaatatca gactcctttt catcaagaag gtttgaggct atggttttac
     2701 cttcaatttc atataatcct gtttcaccaa gatagagttt gtccgttctt attgccagat
     2761 gaggtccctt tatacttttt tccgagtcca ggggaagcag tgttccgctt ttgaaattta
     2821 attctcctga ggccggatct gcgaattcca ggagcctgta ccagaaaatc ggataggatg
     2881 tcctgagatg gaaatccccc caatcctggt ttgttccgat atataccact tttccttttc
     2941 caaaattgcg gtatgcaagt atgggacctt ctttcgcgga tgccaattct actgcgcctt
     3001 cttttattgt tcctttcaaa tacttcgata cgccaaaatc gatatcattt gtcaatccgg
     3061 atcttttcac atctatccgg gatgggtttg cgatccctga aacattaaca ggaagaagtt
     3121 cttttgtgtc caggaatgga aggtcgcttg ctgcttcgaa caccacagtg ccgccgcttg
     3181 tggcatagtt cttaatatct tccatggcgc ccggcaaaag tgaggatctt gtataatcat
     3241 aaagcactac catgccataa tccagtttcc ggggcgccct ttcaaatgaa accttatcta
     3301 tttcgagatt cgggatcgat tcgaatgctt tggaaatgga gctgctcttt ccggaggaat
     3361 cacggagtaa caggatacgt ttgttttttg gatattgaat gcttatgtag agcatattat
     3421 caagcgaaaa atcatcagca ggctcaagtg aaatttttgt agttccggga ttcatatctg
     3481 taactgtgaa gaaatccctg gactttggtt ttattgttct tgagattgtt tttaccgaat
     3541 ccccattcat tatttttata atcgtatctt ccggaatatt attataattc cggacaacaa
     3601 atcccatgct gttatcttca attgaggcat ccactatacc aagattactg ccaccacttc
     3661 ctatttgccg atattccacc gggatatttt ttgattctat tatcttttga acaccgctta
     3721 tatcctgccc tgaaaaatcc gatattatga ccaggttgca ggtcttgttt ccaatcagat
     3781 caagggcgaa cattatcgct tcgttcaaat ccgtgccggt ggccacaggt ttgagttcct
     3841 taagcccatc aatagcctgt ttgctatttt catcagtata taaaagcaca ggcacattct
     3901 gcgcaagtat gaggctgttt ttttcacttt ttccaggata ttccctggca atttccaggg
     3961 cttctgtgaa cctgtccggt ttgacatcgc tggcagacat acttgcggaa gtgtcaagga
     4021 cgattaccgt gcgttcgtat tctgcatttg cgaagtaata gggaccggca ataccaagta
     4081 taaggatagt catggcaaga agctgaatta ataaaagcgg gtctttgata agtttatcaa
     4141 acagggatct taaccttctc ttactggaaa aagaatgcaa aagcaggagt gaggggatct
     4201 taattacccg gggtttgggt tttaacagat acaggataat aaccggcagg aggataagca
     4261 ataaataaag cgccgggcca ttttccaaca tattattctc ttgatatatt catgaaataa
     4321 tttttcacga tctgctggta tatcacaggt attgcttcgc tgtaggcttg cgacgatgaa
     4381 atggcagtat cctcattttg tggaaaagct ggataaacca caggagtttt cctgtccggt
     4441 cctggaacgt ttaattcact tcccgaacca cgattgacca gcaatccctg cgagtcattt
     4501 gcaatactta caaatgaagg ttcatcgaaa atatttccgc ctgaattttt gctctcactg
     4561 ctacttgtta atgtcggggt tctttccgga tctattttct gttcatttat tttctgttcg
     4621 tttatttttt gatcaaaata caatggattt atgatggaac ttgaaaccaa aaaggttaca
     4681 agcaagagaa taattgctat ccttatcccc agcttctttg tttcaaagaa ggaagaatat
     4741 ttgatttttt caatatccgt taagactgat tctgcaagat tttcaagcac aacatttcta
     4801 ttttctttat tatcataaat tgtccttagc cgttcattga gttcgggata ttttttctcg
     4861 atcaacgtca ctgtatttct cctggattta tttctaaatg atacaatgat gttcaggaaa
     4921 atcggtataa taatccagta gggacttatt cccatatacg ttactaaaag gaaacctgca
     4981 agaacgaaaa ttgcagtatc aagtactgtc tcgataagcc ataaatgatc cagaatattt
     5041 tttatctcta ttaccctgta aataaattta tacattctag cggctcttaa gtttatccaa
     5101 ttcagtttgt atttccgata tatcattcaa gagaatatct tttgatgtat tgtcttcaac
     5161 acgagtttta atatggattt tgagatcctc gatctttgcc tgcatatccc cgatatatgt
     5221 ggtatgcctg ttcaatattt cattcgcata gctgctgtta agggtcattg catcatattt
     5281 cccctcgttt tcgaaaagct gctgctggac aattgcaagt tccctgctga cagtctccaa
     5341 ttcttttgtt tttgtttcca gttttgaggt caaaacattt tccctttcat aagaaacatt
     5401 aagatcgctg atatttgaat aaagcgatct ttgatctgaa ctcatgttag caaatgtttt
     5461 gttgtattcc ctgaaagtct gctcataact gtctctcata ttatcatatc ttagctggta
     5521 tagaattgtg gtaccgccaa gtgcaagcaa taaaatcaaa ataatatata ttaattttat
     5581 attgacatct cgttccatta ttattaataa atgcccataa agtataaaag cactgtatat
     5641 ggaaactgtt attgttattg aaattgtgag taggctatat gaaggcttgc agttcatgtg
     5701 aagcatctgt aaagtgcggg ttaaaagatg aggggctttt tgacctgtgc cctaaattca
     5761 aaacctcccc cgatccggga aaaatcgata tgcatccgga tgagacgagc cctgttatcg
     5821 attatgacat cctcaggcgg tattttggat actctgaatt ccgccagctt caaaaggata
     5881 taatccagga tgttctcaag ggaaatgatg tccttgtgct ta
//
LOCUS       DATGBN010000392         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_265589, whole genome shotgun sequence.
ACCESSION   DATGBN010000392 DATGBN010000000
VERSION     DATGBN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265589"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VKL21_09735"
     CDS             complement(<1..330)
                     /locus_tag="VKL21_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HMB46090.1"
                     /translation="MSTSERKIEHLLLCVEKDVEAHKSNTGLRASGFDDIDLVHNCLP
                     EINKKNVYLDTEFLDKKLRAPILIASMTGGHPDTKTVNATLAGAAEELGIGIGVGSQR
                     AAIEDPNI"
     gene            complement(364..1131)
                     /locus_tag="VKL21_09740"
     CDS             complement(364..1131)
                     /locus_tag="VKL21_09740"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HMB46091.1"
                     /translation="MSLIILKIGGSVITEKGSISKAREAEIDRISSEIASFRKDSDSQ
                     LIFVHGAGSFGHPQAMKYRLSEGFDAQGAYLTHASVKSLNSRVVESLNNAGANALPVH
                     PLSSCLLENGKIIDFQVGQIKVMLENGIIPVLHGDVAMDRIKGASVLSGDRIIPYLAI
                     ALNASQIGAGSDVDGVFDDKDTVIKKISQYSFTNIRKNIKGSNSTDVTGGMLGKVSEM
                     LELAKKGISSRIFNASRKGVVSRFLYGEDVGTLIEDK"
     gene            complement(1128..2027)
                     /gene="mvk"
                     /locus_tag="VKL21_09745"
     CDS             complement(1128..2027)
                     /gene="mvk"
                     /locus_tag="VKL21_09745"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HMB46092.1"
                     /translation="MTTTCTAPGKIYLFGEHAVVYGETAIACAVELRTRVRVKRSNEI
                     SIFSDIGTTGLDFDLHPYVSSAIQKSGSPNVSIEISSQIPVGSGLGSSAAVTVATLEA
                     INIECGLLYDKTGIARMAHEIEKEVQGAASPTDTFVSTFGGVIEIPSRKKLDILECGI
                     VIGNTNKGASPGKTAKLVKQVALLKEEYPDPIDAIIKTIGSFAKHGQIMVMQKKYQAL
                     GKLMNVNHGLLDALGVSTMELSTLVYAARNAGAYGAKLTGAGGGGCMVALSDSPRKVA
                     SAIEKAGGTAIISSFALDGVLQV"
     gene            2221..2832
                     /locus_tag="VKL21_09750"
     CDS             2221..2832
                     /locus_tag="VKL21_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46093.1"
                     /translation="MKNSNNETADIGIGTLIIFIAMVLVAAVAAIVLIYSAGELQQKA
                     TITANEAPSKVTTNIFIESVFGDRVNSTMPGLQPGVQSILIRIRPEVGTASMDLRQII
                     MTKMSSDEIEILNYSNISNTVNSFGASTIRDEDGSFTADFPVLNSGDLVDIEFHTLDY
                     TKPILIPRQTITLSINQERGASVTLELTGPNSFGNERYVKLYP"
     gene            complement(2861..3508)
                     /locus_tag="VKL21_09755"
     CDS             complement(2861..3508)
                     /locus_tag="VKL21_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMB46094.1"
                     /translation="MDITSLRGRIGRLVQTHSSSLIHEIERADFSCSRCGWCCHRNFD
                     IRITDNISRPSNAISIFPGDIRRIIKMTGRKWDEVAGPDIYSCFSKGCDVQLVIGWIL
                     KRNDEDNCVFYRNGECTIYSCRPLICRCYPFFMGDEDVEIMHCEGLGKKTLNENALEI
                     GRALKRYEIKKLHSYIRIIEQIGDKLDIATMKSLPEKYSGDVLVFDGESVSRCKL"
BASE COUNT         1040 a          830 c          701 g         1084 t
ORIGIN      
        1 tatgttgggg tcctctatgg ctgctctctg gctgcctaca ccgattccga tgcccagctc
       61 ttctgctgcg ccggcaagcg ttgcattcac ggttttagtg tcaggatgcc cgcccgtcat
      121 tgatgctatc agtattgggg cgcgcaattt cttgtccagg aattcggtgt caagataaac
      181 atttttctta tttatttccg gaagacaatt atgaacaaga tctatatcat caaaacctga
      241 tgcccgcaga ccagtattac tcttgtgcgc ttcaacatct ttttcaacgc ataataataa
      301 atgttcaatc ttcctttccg acgtactcat tttatatccc ttattaaatc aactatatat
      361 aactcatttg tcttcgatca aagttccaac atcttctcca tataaaaatc ttgatactac
      421 acctttgcgg gaagcattga agatacggga gcttattcct ttttttgcca gttccagcat
      481 ctctgatact tttcccagca ttcctcctgt cacatctgtg gaatttgaac cctttatatt
      541 ttttctaata ttagtgaagc tgtactgcga aatttttttg ataaccgtat ctttatcatc
      601 gaacacccca tcaacgtcgc ttccagcgcc aatttgcgaa gcattcaatg caatggcaag
      661 atacggaatt atcctgtccc ctgaaagaac cgatgctcct tttatcctgt ccatcgccac
      721 atcgccatga agcacaggta tgataccatt ttcaagcatc acttttattt gcccaacctg
      781 gaaatcaatt atctttccgt tctcaagcag gcatgagctt aagggatgaa cgggcagggc
      841 attcgcgccc gcattattca aggactctac aactcttgaa ttcaacgatt tcacagaagc
      901 atgggtaaga tatgctccct gtgcatcgaa tccttcgctc aacctgtatt tcatcgcctg
      961 cgggtgacca aatgaacctg cgccatgaac aaagataagc tgtgaatccg aatctttcct
     1021 aaatgaggcg atctcactgg atatcctgtc aatttcggct tcccttgctt ttgatatgga
     1081 acctttttca gttattacgc tcccaccgat cttcaatatt atgaggctca aacctgtaac
     1141 accccatcaa gagcaaaact ggaaattatt gctgtacctc cggctttctc aattgcggat
     1201 gctactttcc tgggtgaatc cgacagggca accatacacc cgccgcctcc ggctcccgtg
     1261 agttttgccc catatgcgcc agcattacgt gccgcatata caagagtgga taattccatt
     1321 gtgctaacac caagagcatc aagcagcccg tgattaacat tcataagttt tccgagtgcc
     1381 tggtattttt tttgcatgac catgatctgc ccatgttttg caaatgaacc gatggttttg
     1441 attattgcat ctatgggatc agggtactct tctttaagta gagctacctg tttcacaagt
     1501 tttgcggttt tcccgggaga tgcgccttta ttggtgttgc cgatcacgat gccacattca
     1561 agaatgtcca gcttttttcg cgaggggatt tcgatcacac cgccaaaagt cgaaacaaat
     1621 gtatcggtag gacttgccgc cccctgcact tccttttcta tctcatgcgc cattcgtgca
     1681 ataccggtct tatcgtatag aagaccgcat tcgatattta tagcctcaag tgtggcaaca
     1741 gttacggcgg ctgatgagcc aaggccggag ccaacgggga tttgcgatga tatttcaatt
     1801 gatacgtttg gagaacccga tttttggata gccgatgaaa catagggatg cagatcaaaa
     1861 tcaagtcctg ttgtcccgat atcggagaat atcgaaattt catttgatct ttttacgcga
     1921 acgcgggtcc taagctccac tgcgcaggct atcgcagttt ccccatatac cacggcatgc
     1981 tcaccgaaca ggtatatttt tccaggggct gtgcatgtag tggtcatcaa aattcttaga
     2041 gtcccaagat gcgatatgta aatataagca ttgttagtga atttaaaaac aagtattcct
     2101 gaaaatcctt ttcattaact catgcgaaat aatttcttag tgttcattat gggaatatag
     2161 aaaaaaaata tatattgtat aagataataa gaattgaaga aaaaatggac agaaatttta
     2221 atgaaaaact caaataatga aactgctgat attggtattg gcactttgat aattttcatt
     2281 gcaatggtat tagttgcagc agtggcggca atcgtgttga tttattcagc aggggagctt
     2341 cagcaaaaag caacaattac agcaaatgaa gccccttcaa aggtcacaac aaatattttt
     2401 atcgagagcg tgttcggaga cagggtgaac tctactatgc caggcctcca gccaggcgtt
     2461 caaagcatat taatccgtat cagaccagag gtaggcactg cttcgatgga tttgaggcaa
     2521 ataattatga ctaagatgag cagcgatgaa attgagattt tgaattattc aaatatcagc
     2581 aatacagtaa attcatttgg cgcatcaacg atacgcgatg aagacgggtc atttacggca
     2641 gattttccgg ttcttaacag cggagattta gtggatattg aatttcatac tcttgattat
     2701 acaaaaccaa tcctaatacc aaggcaaact ataactctat ctataaacca ggaacgtgga
     2761 gcatcggtaa ctcttgaatt aaccggacca aactcttttg gaaatgaaag atatgttaag
     2821 ctttatcctt gattcatgaa tgatgcatga attaatttct tcataatttg caccttgaaa
     2881 ccgattcacc atcaaaaaca agaacatcac ctgaatattt ttccggaaga gacttcatag
     2941 tggcgatatc taacttatct cctatctgct cgattattcg gatatagctg tgcaatttct
     3001 ttatttcata acgcttcagc gccctcccga tttcaagggc gttttcattt aaggtctttt
     3061 tgcccagtcc ctcgcaatgc ataatttcaa cgtcttcatc ccccataaaa aaaggataac
     3121 atctgcagat caagggacga caactgtata tcgtgcattc cccgtttctg taaaatacgc
     3181 aattatcctc atcattgcgc ttcaatatcc acccgatcac cagttgtaca tcacaccctt
     3241 tcgagaaaca tgaatatata tccggtcctg ccacttcatc ccatttgcgc cctgtcattt
     3301 tgattatgcg cctgatatca cccggaaaga ttgaaatggc attggagggg cgggaaatat
     3361 tatccgtgat cctgatatcg aaatttctgt gacagcacca gccacaacga gagcatgaaa
     3421 aatcggctct ctcgatttca tggataagcg atgaagaatg ggtctgaaca aggcggccta
     3481 ttcttcctcg aagagaggtg atgtccatgg ataaacatca gattaggggg atattaagtt
     3541 atccacaata ttagttataa atagtttcat cttcaaatag cgtgttcgat acatgaaact
     3601 gactatcaat aaatctgaaa taaaaggcac agttaatgcc cctccatcca aaagc
//
LOCUS       DATGBN010000393         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_265719, whole genome shotgun sequence.
ACCESSION   DATGBN010000393 DATGBN010000000
VERSION     DATGBN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265719"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..187)
                     /locus_tag="VKL21_09760"
     CDS             complement(26..187)
                     /locus_tag="VKL21_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46095.1"
                     /translation="MKKMKIIVEKHPEGYVAYPIGLKGVVIGEGDTHEEALADVKSAI
                     KFHIETFDN"
     gene            complement(184..330)
                     /locus_tag="VKL21_09765"
     CDS             complement(184..330)
                     /locus_tag="VKL21_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46096.1"
                     /translation="MVKTTVQISKDTKWRIVIPEIVRTVEELKPGDFIGTDVKKIEKQ
                     KTTI"
     gene            505..657
                     /locus_tag="VKL21_09770"
     CDS             505..657
                     /locus_tag="VKL21_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46097.1"
                     /translation="MNNVESVKKAFREARIAGEKKLSQGKITFDEFADQMLSFEARLR
                     SMGVVL"
     gene            654..791
                     /locus_tag="VKL21_09775"
     CDS             654..791
                     /locus_tag="VKL21_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46098.1"
                     /translation="MNYFEKIILIKYVCGHKKYGIDTYQAFVTKNEVKCPKCTNNGGR
                     I"
     gene            788..946
                     /locus_tag="VKL21_09780"
     CDS             788..946
                     /locus_tag="VKL21_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46099.1"
                     /translation="MTPPLCDMECSCEDCGDRQACDIYFKEIGYQLLQASNEEDWHEK
                     RFAEKEGL"
     gene            946..1431
                     /locus_tag="VKL21_09785"
     CDS             946..1431
                     /locus_tag="VKL21_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HMB46100.1"
                     /translation="MPSSDRIKEAMETLLKIFKEDNLEKVARAVFRGGDIPADKWSFL
                     NRVLMYLNETDDARGFRQWQQVGRYVKKGSKAFYIIGPVTKKITEEKSLESGEKVREE
                     KQIIAGFKAIPVFRVEDTEGEPIIREDFKVNIPYEFNGIIQELALKVQPGMMKKGSCC
                     T"
BASE COUNT          512 a          301 c          339 g          431 t
ORIGIN      
        1 tctaatgatg atcaattgat ggttttcaat tatcaaacgt ctcaatatgg aatttgatgg
       61 cagattttac atcggcaaga gcttcctcat gggtatcgcc ttctccaatt acaacgcctt
      121 ttaagccgat aggatacgcc acgtaccctt caggatgctt ttcaactatt attttcattt
      181 ttttcaaatt gtagtcttct gcttttcaat cttctttacg tcagttccta taaaatcccc
      241 tggtttcaac tcttccactg ttcggacgat ttcaggaata acgattctcc attttgtatc
      301 ttttgaaatt tgaactgttg tctttaccat aatagtacag tgtaccacta tatatttaaa
      361 tattgtgtta tggggtacaa cgtaccataa actatataag caataaagtc atagtagaat
      421 atgtaaggaa aatgagcaag aacctccgcg gtgctagtaa cacccggagg cgtggctcat
      481 tcctgtcaac gaggaatgaa caaaatgaac aatgtcgaat cagtaaaaaa agctttccgt
      541 gaagcaagaa tcgcaggcga aaaaaagtta tctcagggaa aaattacctt cgatgaattc
      601 gctgatcaaa tgctgagttt tgaggcgaga ttaagaagca tgggagtagt tctatgaact
      661 acttcgaaaa aatcatcctg ataaaatatg tctgcggaca taaaaaatac gggattgata
      721 catatcaggc ctttgtaaca aaaaatgaag taaaatgccc gaaatgtacc aataacgggg
      781 ggagaatatg acccctcccc tttgcgatat ggagtgcagc tgcgaggact gcggagatag
      841 gcaggcctgt gatatctatt tcaaagaaat aggctaccag ctattacaag cgagcaacga
      901 agaagactgg catgaaaaac gatttgcaga aaaggagggt ttataatgcc ctcctcagat
      961 cgcataaaag aagcaatgga aacattgctt aaaattttta aggaagataa ccttgaaaaa
     1021 gtagcaagag cagtattcag gggcggcgat ataccagcag ataaatggag cttcttaaac
     1081 cgtgttctga tgtacctgaa tgaaacagac gatgcaagag gcttcaggca gtggcagcag
     1141 gtcggaaggt atgtaaagaa gggatcaaag gccttctata tcatcgggcc cgtgacaaag
     1201 aagatcacag aggagaaaag ccttgaatcc ggggaaaaag tcagagagga aaaacagatc
     1261 attgcaggct ttaaggctat tcctgttttc cgggtcgagg atacagaggg agaaccgata
     1321 ataagagaag acttcaaggt aaatatcccc tacgagttca acggcatcat tcaggaactt
     1381 gcccttaagg ttcagcctgg gatgatgaaa aaaggctcat gctgcaccta aaatctgatc
     1441 ctgtttttgt tcctttttaa tgcaagatcc aaatgcttct tttctcattg actgaagaga
     1501 ctgccctata ttgtccttta accaaatgag cacttctttc gtgggaacta cctttagagc
     1561 tttttcaacg acatcttttg ttg
//
LOCUS       DATGBN010000394         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_382145, whole genome shotgun sequence.
ACCESSION   DATGBN010000394 DATGBN010000000
VERSION     DATGBN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382145"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..591)
                     /locus_tag="VKL21_09790"
     CDS             complement(340..591)
                     /locus_tag="VKL21_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46101.1"
                     /translation="MATVHADLTFQKNNYIWGPIGRFGWKYSSTWHSGNPFKEIIKEA
                     NSKKEKWPPLEAGLFGGDYERFNKVISEYENIISKLHWH"
     gene            complement(567..1517)
                     /locus_tag="VKL21_09795"
     CDS             complement(567..1517)
                     /locus_tag="VKL21_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46102.1"
                     /translation="MPNHSIFTGHLLQALEGNAETTDGIISANSVMSYVYDKVAKDQY
                     SRQTPHYGYLDGDGDFIFKSPNIGTLLEEKEKDLDILVSVTSPETKLFPQVSENLVDT
                     IKEYLSDPKYKIKLHDLIVQKIREVLIQLSEKSLKDMNKPFTIEELNRRLEKYESIIQ
                     DLQSIIVCLAYWGDEQHHSIMQKIMARISEELGPRDGLVIWTSLRPYPLIILLYSAGI
                     AAVASRKYHTLATLFMTNVSSLRDSSTSNELIMAVGKVILDLTEVFKKLSGNERYYTP
                     RSEYLFKMFQPMLDDLLFLGQDYENFSIFSRFFWQQFMLT"
     gene            complement(1546..2085)
                     /locus_tag="VKL21_09800"
     CDS             complement(1546..2085)
                     /locus_tag="VKL21_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMB46103.1"
                     /translation="MKNLELKSEYTESRALIIGINGYKHASPLGYAVNDAQEFAILLI
                     ERFNFINKNVNLLIDEKATRDAIMQSYLSFATSDVKANDRIVIFFAGHGHTQTGHRGE
                     VGFLVPYDGSPADLSSLIRWDEFTRNTELIPAKHILFIMDACYGGLAVTRALHLGSMR
                     FLKDMMLRFARQVLNCWKS"
     gene            complement(2462..2821)
                     /locus_tag="VKL21_09805"
     CDS             complement(2462..2821)
                     /locus_tag="VKL21_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46104.1"
                     /translation="MLGTISVDKKEDKYSIIYFNRTDSPRDERDAMIEIDDVLLFIEE
                     ILNGINKLFDHISVEILKTIDPNKRCPISFGVQDGSLCVKGLNIIEFILNKPGECATW
                     HMGCNKHGKTCFAEISH"
BASE COUNT          891 a          600 c          463 g         1031 t
ORIGIN      
        1 acataatctg acgtatctta ttaactactt cgcttttgcg ttcatgaatg tcattcaatt
       61 gtttcagagc atcgctaata tcaatgggtt cttcttcatt cactgggagt aatttagcga
      121 catattgata aatatcaaat gaaaaaccat tttctgcgat cttttctata ggtgccaccg
      181 aactatcata gcctttaaac cttttaaaat tatctactat ttattatttt tgaatatttt
      241 cgtagagtgc caaattttga gttctacatt ttctggaaat cctttgaagg catttattat
      301 tttcccttta tccccccacg catgttgctg aaaacttgtc taatgccaat gtaatttcga
      361 aattatattc tcatattcgg atattacttt gttaaatcgt tcataatctc ctccgaagag
      421 acctgcttct aaaggaggcc atttctcctt tttagaattt gcttccttaa taatttcctt
      481 aaaaggattg cctgagtgcc atgtgctgct atacttccat ccaaatctcc ctataggccc
      541 ccagatataa ttattttttt gaaaagttaa gtcagcatga actgttgcca aaagaacctc
      601 gaaaatatcg aaaaattttc gtaatcttga ccaaggaaaa gcagatcatc taacattggc
      661 tgaaacatct taaacaaata ttcacttcga ggtgtatagt acctttcatt cccagataat
      721 ttcttgaaaa cttctgttag atctaaaata acctttccga cagccattat tagttcattc
      781 gaagtcgatg aatctcggag ggagctaaca tttgtcatga aaagagttgc aagagtatga
      841 tattttcgag aagctactgc agcaatgcct gcactataaa gaagaataat taacgggtac
      901 ggcctaagac ttgtccagat gacaagccca tccctagggc ctaactcttc actaatacga
      961 gccattattt tttgcattat tgaatgatgt tgttcatctc cccagtaagc aaggcatacg
     1021 ataatcgatt gcaagtcctg tattatagac tcatatttct caagtcttct gtttaactcc
     1081 tctatggtga agggtttgtt catatctttt agactttttt ctgaaagttg tataagaact
     1141 tcacgaattt tttgtacaat caaatcatga agtttaattt tatacttagg gtcagacaaa
     1201 tattctttta ttgtatcaac caagttttct gaaacctgag ggaatagctt ggtctccggt
     1261 gaagttaccg atacaagtat atcaaggtct ttttccttct cttctaataa agtcccgata
     1321 tttggactct tgaaaataaa atctccgtca ccatcaagat aaccataatg tggtgtctgt
     1381 ctagaatatt gatcttttgc aactttatca tatacatatg acattacact atttgcagaa
     1441 ataatcccat cagtagtttc agcattacct tctaatgcct gcaaaaggtg gccggtaaaa
     1501 atggagtgat ttggcaaagg acctccagaa tcggcaactg cttcgtcagc ttttccagca
     1561 gtttaatact tggcgtgcaa atcttagcat catatctttg agaaatcgca tactacctaa
     1621 atgtagagca cgagtaacag caagcccacc gtagcaagcg tccatgataa acaaaatatg
     1681 ttttgctggt atgagttccg tgtttctggt aaattcatcc catctaatca gagatgataa
     1741 atctgcggga ctaccatcat atggaacaag aaaaccaacc tctccacgat gaccagtctg
     1801 ggtatgacca tgacctgcaa aaaaaattac aattctgtca tttgccttta catcactggt
     1861 ggcaaacgag agatatgatt gcatgattgc atctcgcgtc gccttctcat ctataagtaa
     1921 atttacgttt ttatttatga agttgaatct ttcaataaga agtatggcaa attcttgtgc
     1981 atcattgacc gcgtatccca aaggggaagc atgtttgtat ccattaatac caatgattaa
     2041 tgcccgactc tcagtatatt ccgatttaag ttcaagattt ttcatttctt ttatttattt
     2101 ttctgcaata gcagattact ttttcatatt cgaattctgt tcgtatattt ttccttttgc
     2161 ctgaacgcct attcccttat cctcaaaaca ccttgcatta tgttattcga aggccttttt
     2221 attctgatta aatcttaatt actcacatat gtataagagt tcctcattct actgaatgaa
     2281 aaacttaatc gtttttagga gtctattttg tgatgagcac ctattcttag tgcttgaaaa
     2341 aggttcttct ggattcctat cgtctcaccg agcatgaaac accaccatct tcagtcacga
     2401 acctcggatt ccgctatcgc tcaaatccga ggcatctgaa gagtgcataa gaatccacaa
     2461 attaatgact tatttctgca aaacaggttt ttccatgctt attacaaccc atatgccaag
     2521 ttgcacattc accgggttta tttagtatga attcgattat attcaatcct tttacacata
     2581 acgatccatc ttgaacacca aacgagatgg ggcaacgttt atttggatca atggttttca
     2641 aaatttcaac tgaaatatga tcgaataatt tattaattcc gttcagaatt tcttcaataa
     2701 acaacaacac gtcatcaatt tcaatcattg catctctctc gtcccttggt gaatcggttc
     2761 gattaaaata tattatggaa tatttatctt cttttttatc tactgaaata gttccaagca
     2821 aaaagtgtgt ttcttctgac cgtaacgtca gattgtgtaa aaactatttt ttaagtctgt
     2881 ttgatttcca ctccccaatc aaacccttat gaactgcctt tcatgccaaa atcatgaaaa
     2941 atggtatttt tctatggaat tctatgtcag aacctcttga ttttt
//
LOCUS       DATGBN010000395         8178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_575987, whole genome shotgun sequence.
ACCESSION   DATGBN010000395 DATGBN010000000
VERSION     DATGBN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575987"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VKL21_09810"
     CDS             complement(<1..132)
                     /locus_tag="VKL21_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HMB46105.1"
                     /translation="MKTCIMCGGEGTRLRPLTFDRPKPMIPLLNKPSLVHLVEHLAIE
                     "
     gene            complement(211..1941)
                     /locus_tag="VKL21_09815"
     CDS             complement(211..1941)
                     /locus_tag="VKL21_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46106.1"
                     /translation="MVSIDIILLSVSTYERNTIIFSAVFTAIIPIAAMIYVSEYPKIH
                     AKFLKIHTLGDIPEIQSYIVMSMKLVPNMERAIIFAAENSFRPLARDLKKLIWDIHIR
                     TFSNMDEALIAFSNQWGKNSEYFKRSIHLVKSSLSEPDEAQRIMTLNKSLDIVLEGTE
                     NMMDSFAAKLKTPTYVLFSIFILIPLALVALIPAVSIVGVRIDTMTLVLIYNIGLPIF
                     TFIYSEFILMQRPATFSPPKISVKHPELSNISSTRIKIAIVTLIAGTAIGFSGYILLY
                     YSNPFNIISKDAMEGLILPTFPVVWAIAAMITIYCLGVYIPYKKIRDDIKQIEREFAD
                     AMFILGRRISEGRSAEEAFMHTSETMKGSKIGEAFADISRNLSSMRTTLHGAIFNEEY
                     GAFRDIYSDRVHTIMKLLTESVHRSHEAAGMAIIKLADHLKELQDVEQNIIRTLFDVT
                     STMRSTAIIFAPLIAGVTLALSEVIQKILLNISKESRNLPDEYNIGDFMKEAGTGMTQ
                     SVPPDIFLLVVGIYMILLVIILTRFAGGIEYGDDRPQFMYDLGQTLPMSIMVFTVTTI
                     VSRMIFGSMV"
     gene            complement(2152..4194)
                     /locus_tag="VKL21_09820"
     CDS             complement(2152..4194)
                     /locus_tag="VKL21_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HMB46107.1"
                     /translation="MENLDFLYSLARFINNINSYKNSKHGEECKIYLKQWNEWLLPII
                     NSSTSDPVKAYSELSEKIKALQRSDLSDIGFKIETEKCRVFFLSLLEKMISGVPLMAD
                     RIKSQMSDSDYYGNIKSLVRPGFSTSRIYTSPPSNTEFLERYEVQRSGGRIMPITIYK
                     LTDRPESLYFTIPDEYNNMHPIELEVIETARKKLMRHRPKNINFAEPANSREYFEKLG
                     MQMISEEAREKDLKLTPDEMKVLSDILAKYTTGLGILEDVLSDERVTDIYVNSPADIN
                     PIHVVVDGEECSSNIYLSQDDIDSMITRFRAISGRPFGEANPVLDMDLSEYRTRVSVI
                     GDPLSSGGLAYAFRKHARNPWTLPKLINTGSITALAAGLLSFLMDGQSSILVTGGVGS
                     GKTSLLCAMLLEIPQKYRILIIEDTPELPIENLQKLGCKIQGMNTKASVGGTNIEVNP
                     ETALRAALRMGNATLVLGEVRGPEVKVLYEAMQVGAAGNSVIGTIHGASTRAVYERIV
                     NSLGVPAASFRATDAVVVSQNVRLSGTMKKKKRVVEIAEVTGGQWEEHPDADDIFNDI
                     MVFDAVRDKLIATDLLDRGGSELVSKIARKWGMSIDEASINIKIRALIKQKIAAVGDK
                     HPEFMESDMVIKANNAFCLFCDQIQNDNGKVDFHEVYKRWMSWYFDFVDRNKKMAG"
     gene            4654..5934
                     /locus_tag="VKL21_09825"
     CDS             4654..5934
                     /locus_tag="VKL21_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB46108.1"
                     /translation="MNKIIPHDLEKSGVTQKQYVIGRRDINTSLGTLEVGRYLALDKS
                     MGSRVHMDALRPHAILICGKRGYGKSYSMGIIIEELATLPPEVNIAALVIDTMGIFWT
                     MRHPNNKENEILSKWGFSPVGINAELFVPAGSLEHYKKMNIGAKPFSISPPELSGYDW
                     CSLFGFDPISQIGVFLLKTIDGIKEKTSEFSLANIIDHINTDEEVDMTLRSAAKNYFR
                     SAISWGIFHEKGIILSDIIQKGKVAILDMSSIDNQNIKAIAVKILGSKIYNESIRSRR
                     IYEQREMGENPVDEVIPLVWMFVDEAHLFLPVNGETPATSVLVNEWLRQGRQPGLSII
                     FATQRPAALHPDVMSQSDIIICHRITAQDDISALEAVRPTYMRERIGDSLKKMGSEKG
                     VAFIVDDNSESTHIVRMRPRKSWHGGDEPSAIKK"
     gene            complement(5944..6102)
                     /locus_tag="VKL21_09830"
     CDS             complement(5944..6102)
                     /locus_tag="VKL21_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HMB46109.1"
                     /translation="MEKWKCTLCDYVYDPAAGDPDSGVAPWTAFEDIPDGWVCPWCGA
                     AKDLFKKM"
     gene            6222..7202
                     /gene="mer"
                     /locus_tag="VKL21_09835"
     CDS             6222..7202
                     /gene="mer"
                     /locus_tag="VKL21_09835"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018537 - coenzyme F420-dependent
                     N5,N10-methenyltetrahydromethanopterin reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HMB46110.1"
                     /translation="MFGIEFVPADPVLKLAHYTKLAEENGFDNVWITDHYNNRDVYTT
                     LAVLAMNTNRIKLGTGVTNPYTRNIAITAQSIGAINEISGGRAILGIGPGDKATFDAM
                     GIEWVEPMTTIAESITALRGFFGGKKVSQDGKRIKVGGAKLAFKTGNIPIYMGAQGPK
                     MLELAGKVADGVLINASHPKDFEFAIKQIALGAKAAGRDPKSVDVGAYACFSIHKDAE
                     KAKGAAKVVVAFIVAGSPDTVLERHGIDVGAKKTIGDAIAKGDFGGLNSLVSDNMIDS
                     FSICGTPADCKAKVEALLKIGVTQIVAGSPIGPDKETAIKLIGKEIIGGK"
     gene            7204..>8178
                     /locus_tag="VKL21_09840"
     CDS             7204..>8178
                     /locus_tag="VKL21_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HMB46111.1"
                     /translation="MEAKSDSAVAGPLTERGILKSDQDKVLIRLGNMYAPNVGLPKIA
                     DVGEYNYCCSCGTCEAICPMNAPIVRKDQVDISKEKNNYNKMELTTEVVFKNVNPLEV
                     DVNPCVNCYSCERICPILDGFPADEFNNIREMKAGKSKTLHGQDGAIVSQILKSLLEQ
                     GEIDCAIGIVRNEKWETKVVMFTSPEDITKASGTKYTYQPVLSSLRDIHRAFVDTYVQ
                     GLKKYKKVALVGVPCQAHGAKLFRENFGRIELIIGLICMESFSEQIMLNEMVPKIMGV
                     DIRDVKKMNFNKGKFIVETDKEVKEVPIKVVAPLARKGCHHCQDYTSYF"
BASE COUNT         2489 a         1705 c         1667 g         2317 t
ORIGIN      
        1 ctcgatggcc agatgttcca caagatggac taatgatggt ttgttcaaca gggggatcat
       61 aggctttggc ctatcaaaag ttagcggccg aagccttgtt ccttcgccac cgcacattat
      121 acaagttttc attcaagcac ctttcttgga attggtattg gagtatatag atataactga
      181 atagagattt ctttacattg tgatgcactt tcaaaccatt gaaccaaaaa tcatccttga
      241 aacgatcgtc gtaaccgtga aaaccataat cgacattggc agcgtctggc ccagatcgta
      301 cataaattgg ggtctatcat ccccatattc gatcccaccc gcgaatcgcg taaggataat
      361 cacgagaagt atcatgtata ttccaaccac aagcagaaaa atatcaggag ggacgctctg
      421 ggtcattcca gtgccggctt ctttcataaa atcacctata ttgtattcat ccggtagatt
      481 ccgggactct tttgatatat tgagcaatat cttctggata acctcagaaa gagcaagcgt
      541 aacacctgcg ataagaggag caaaaataat agcagttgac ctcatagttg aagtgacatc
      601 aaataatgtc cgtatgatat tttgctctac atcctgtaat tccttaaggt ggtccgcaag
      661 ctttatgata gccatacctg cagcctcatg acttctatga acgctttctg tgagtagctt
      721 cattatcgta tgtaccctat ccgaatatat atccctgaac gcgccatatt cctcattgaa
      781 aatagcgcca tgtaatgttg ttcgcatgga tgaaaggttt cttgaaatat ctgcaaaagc
      841 ttcccctatc tttgaccctt tcattgtctc tgaagtatgc ataaaagctt cttctgccga
      901 ccttccttca gatatccttc gaccaagtat gaacatggca tcagcgaact cacgttctat
      961 ttgttttata tcatcacgta tttttttata aggtatatat actcccaggc agtaaatcgt
     1021 gatcatggca gcaatagccc agacaaccgg gaacgtgggc aaaatcaatc cttccattgc
     1081 atcttttgat atgatattga atggatttga ataataaaga agtatatatc ctgaaaaacc
     1141 aattgctgtt cccgctatca atgtgacaat ggcgatcttt atcctggttg aactgatatt
     1201 ggagagttcg ggatgtttga cagatatctt tggcggtgag aaagtggcag gccgctgcat
     1261 taagataaac tcagaataaa taaatgtgaa aataggcaat ccgatgttgt aaattaaaac
     1321 aagggtcatg gtatcgatcc ttactccaac aatgcttaca gccgggatca aagcaactag
     1381 agccagaggg atcaatatga atatagaaaa tagaacataa gtaggtgttt ttagtttagc
     1441 tgcaaatgaa tccatcatgt tttccgttcc ttcaagaaca atatccagtg atttgtttag
     1501 agtcatgatc ctttgagctt catcgggttc gctcagggaa ctttttacaa gatgtatcga
     1561 acgcttgaaa tattcactat ttttccccca ttggtttgaa aatgcgataa gcgcctcatc
     1621 catattgcta aatgttcgaa tatgaatgtc ccagataagt ttcttcaaat cccttgcaag
     1681 cggcctgaat gaattttcag ctgcaaaaat aatagctctc tccatattag gaacaagttt
     1741 catagacatc acgatatagc tctggatttc cggaatatcg ccaagagtat ggattttcag
     1801 gaattttgca tggatctttg gatattcact cacatatatc attgcggcaa tgggaataat
     1861 cgcagtaaat accgctgaaa aaattatcgt gtttctttcg taagttgaaa cgcttaaaag
     1921 aataatatca atactaacca ataaaatgaa taaaaacagg ctgcaagtat atgaaagcaa
     1981 atgggtttcc catggctcaa gttcaaatcc ggaaaaatct attgcatcaa ggaaatcttt
     2041 gcttactgtt ttctggcttt tcttcctgta agcatcgccc agaagccatc caaagtattt
     2101 ggctgaaaaa cggcaggcgc gacaataaat atgttgttga tcgttaataa ttcatcccgc
     2161 cattttctta tttctatcca caaaatcaaa gtaccatgac atccatcttt tataaacttc
     2221 atgaaaatcc acttttccat tatcgttctg gatctggtca cagaacaggc agaaagcgtt
     2281 gttggctttt attaccatgt cagattccat gaactccgga tgtttatccc caacggctgc
     2341 aattttttgc ttgatcaatg ctctgatctt aatgttaata gatgcttcat cgatgctcat
     2401 accccattta cgagcgatct tacttacaag ctctgatccc cccctgtcca gcaaatctgt
     2461 agctataagc ttatccctga cggcatcaaa taccattata tcgttgaaaa tgtcatctgc
     2521 atcaggatgt tcttcccatt gtcccccggt tacctctgct atttccacaa ctcttttttt
     2581 ctttttcatt gttccgctca gtcttacatt ctgggataca acgacagcat cagtcgccct
     2641 gaaagatgca gccggaaccc caaggctgtt gacgatccgt tcgtatactg ctcttgtgga
     2701 tgcgccgtgg atggtaccga tgacagaatt accggcagcc ccaacctgca ttgcctcgta
     2761 caatactttc acttcaggac ctctcacctc accgagtaca agagtggcat ttcccatccg
     2821 aagggcagca cgaagagccg tttcgggatt gacctcgatg ttcgtgcctc caacagaagc
     2881 ttttgtattc attccctgga tcttgcaccc aagtttctgc agattttcaa ttggaagttc
     2941 gggagtatct tcaatgatca atatcctgta tttctggggt atttcaagaa gcatggcaca
     3001 tagaagggat gtttttcccg aaccaacacc acccgtgaca agaatagaag actggccgtc
     3061 catcaggaaa cttaataaac ctgcagccag cgcagtgatc gaacctgtgt ttatcagctt
     3121 gggaagcgtc catggatttc gtgcatgttt tctgaaagcg taagccagac cgccggatga
     3181 taacgggtct ccgattactg atactcttgt cctatactcc gaaaggtcca tatcaagtac
     3241 aggatttgct tccccgaatg gtcttccgct tatcgccctg aaccgcgtaa tcattgaatc
     3301 gatatcgtcc tgtgataaat agatatttga agaacattct tctccatcaa caacaacatg
     3361 gatcggattt atgtcagccg gagaattcac atatatgtca gtaacgcgtt catcagaaag
     3421 cacatcttcc agtatcccca atcctgttgt atattttgcc aggatatctg ataatacttt
     3481 catttcatca ggggtcagtt ttaaatcctt ttcacgagct tcttctgaaa tcatttgcat
     3541 cccaagcttt tcgaaatatt ccctggaatt ggcaggttct gcaaaattga tattctttgg
     3601 cctgtgtctc atcaatttct tcctggccgt ttctatgacc tccaattcta ttggatgcat
     3661 gttattatat tcatccggaa tcgtgaaata caggctttca ggcctgtccg taagcttata
     3721 aatcgtaatt ggcatgatcc ttccccctga tctttgtact tcatatcttt caagaaattc
     3781 cgtattcgat ggcggggagg tatatatcct tgaggtggaa aagccgggtc ttacaagcga
     3841 ctttatattc ccgtaataat ccgaatccga catctggctt tttatccgat ctgccattaa
     3901 aggtacaccg gagatcattt tttccaataa tgagagaaaa aagactctgc atttttcggt
     3961 ttcaattttg aatccaatat ctgataggtc ggatctttga agagccttga tcttttcact
     4021 taattccgag tatgctttta caggatcgga tgtacttgaa tttatgatgg gaagaagcca
     4081 ttcattccat tgttttaaat atattttaca ttcttctccg tgtttagaat ttttataact
     4141 gttgatatta ttaataaatc gcgccagtga atataaaaaa tccagatttt ccatttcata
     4201 gtcccgctca aaaagatgag atagaacaag acggtttgcc ctgggttcag agtttagtat
     4261 ttgaaatata tattccctgc atgttgaatc tgtgagggat gaagtacctg atctgcagtt
     4321 cttgcaatca agaattataa tacgctggtt tttgtatttt cgaaccttat aagaacaagg
     4381 gtttttgtgg agtttctttt tccctgttaa tgatctaatt gttaatatca aattatttat
     4441 tataatttca ctcactccag gatttacctt gcagtgacct tctgtttata taaatttatc
     4501 ctattcaata tataataatc attactatta ttataatata aattaagatt atcatgatta
     4561 ttacaagtat ttacaatata aaataataac acacatatta aatactataa accgcctaca
     4621 aacgtcttgc agaggatata attgataaat accatgaaca aaataatccc gcatgattta
     4681 gaaaaatcag gtgtcaccca aaagcaatac gtaatcggta ggagggatat caatacaagc
     4741 ctgggcacac tggaggtggg aagatacctg gctcttgaca aatcaatggg ttcacgagtt
     4801 catatggatg ctctaagacc ccatgccata ctcatatgcg gtaaacgtgg atacggcaag
     4861 tcctattcaa tgggaataat aatcgaagaa ctggcaacac ttccaccgga agtgaatatt
     4921 gccgctcttg ttattgatac aatgggaatt ttctggacaa tgcgtcatcc aaacaataaa
     4981 gaaaatgaaa ttttatcaaa gtggggtttt tctcctgtgg gaatcaacgc agaactattt
     5041 gttccggcag gaagccttga acattataaa aaaatgaata ttggcgcaaa gccattttca
     5101 atttcacccc cggaactctc aggttacgac tggtgttcat tatttggttt tgatcctata
     5161 agccagatag gtgttttcct tttgaaaaca attgacggga taaaagaaaa gacatccgaa
     5221 ttctcattag cgaatattat tgatcatata aacaccgatg aggaggtaga tatgactttg
     5281 cgaagcgccg caaagaacta tttccgatcg gccatttcat gggggatatt tcatgaaaaa
     5341 gggatcatcc tttccgatat tatccagaag ggaaaagttg ccatccttga catgagctcg
     5401 atcgacaacc agaatatcaa agcaattgcc gttaagatac ttggctctaa aatttataat
     5461 gagagtataa gatcaagaag gatatatgaa caacgggaaa tgggagaaaa tccagtcgat
     5521 gaagtcatcc ccctggtctg gatgttcgtt gatgaagcgc atttattttt accggtgaat
     5581 ggagaaactc ctgcaacaag cgtacttgtg aacgaatggt taaggcaggg aagacagccc
     5641 ggtctttcaa taatttttgc aacccagagg cctgctgctc ttcatcctga tgtcatgtcg
     5701 caaagcgata tcataatctg ccatcgtatt acagcacagg atgacataag cgcgcttgaa
     5761 gctgttcgtc ccacatatat gagagagaga ataggtgatt ccctgaaaaa gatgggatca
     5821 gaaaaaggag ttgctttcat cgttgatgat aattccgaat ccactcatat tgtccggatg
     5881 cgcccacgga aaagctggca tggaggcgat gagccaagcg ccatcaaaaa ataattctta
     5941 aatttacatt ttcttgaata aatcttttgc agcgccgcac caggggcaaa cccagccatc
     6001 cggaatatct tcaaaagctg tccacggcgc tacacccgag tccgggtcac cggcagccgg
     6061 gtcatataca tagtcgcaaa gcgtacattt ccatttttcc atataattat gattcactcc
     6121 tcaaaactta acaattacgc ttcaaaattc cctgagcccg aacaaaacca atatgctttt
     6181 aaataaagaa agacgaatgt cgtaaaaacg taggaggaaa tatgtttggc attgaatttg
     6241 taccggcaga tccggttcta aaattagcac actatacaaa gctcgcagaa gaaaatggtt
     6301 ttgacaatgt ttggattaca gaccactaca acaatcgtga tgtctatacc acacttgcag
     6361 tactggcaat gaacacgaac aggataaaac tgggaacagg agtaacaaat ccatatacaa
     6421 ggaatattgc catcacggcc cagagcatcg gagcgatcaa tgagatatct ggcggccgtg
     6481 cgatacttgg tatcggccct ggtgataagg ccacttttga tgcaatgggc attgagtggg
     6541 tcgagcctat gacaacgatc gccgaaagta taaccgcact ccggggattc tttggcggca
     6601 agaaagtctc acaggatgga aaacgcataa aagtaggagg agcaaaactt gcttttaaga
     6661 cagggaatat tcctatctat atgggagcac agggccccaa gatgctggaa cttgcaggta
     6721 aggtcgctga cggagtgctc ataaatgcat cccatcccaa agacttcgaa tttgcgataa
     6781 aacaaattgc gctcggcgca aaagcagcag gacgcgaccc caagagcgtt gacgtaggcg
     6841 cttatgcatg cttctcgatc cataaagatg cagagaaagc aaagggcgca gcgaaagtag
     6901 tggtcgcatt catcgttgca ggttcaccag ataccgttct ggaaagacat ggcattgatg
     6961 ttggagcaaa gaagaccatc ggcgatgcga tcgcaaaagg cgactttggc ggattgaact
     7021 ctcttgtttc tgataatatg atcgattcat tctccatatg cggaacacca gctgattgca
     7081 aggcaaaagt cgaagcgctc ttgaaaatcg gtgttaccca gatcgttgca ggctcaccaa
     7141 ttggtcctga caaggaaact gcaatcaaat tgataggaaa ggaaatcatc ggaggaaagt
     7201 aaagtggaag caaaatcgga cagtgctgtc gcaggacccc ttacagaaag gggcatcctc
     7261 aaatccgatc aggataaggt tctcatcaga ctgggcaata tgtatgcccc caacgtggga
     7321 ttaccaaaaa ttgcagatgt tggcgaatac aactactgct gctcctgcgg gacatgcgaa
     7381 gccatctgtc cgatgaatgc gcccatagta cggaaggacc aggttgacat ttcaaaggaa
     7441 aagaacaatt ataataaaat ggagttaaca actgaggttg ttttcaagaa cgtcaatcct
     7501 ctggaagtag atgtcaaccc atgtgtgaat tgctattcat gtgaacgcat ctgcccaata
     7561 ctggatggtt tcccggcaga tgaattcaat aatataaggg aaatgaaagc cggaaaaagc
     7621 aaaacacttc acggccagga tggagcaata gtttcccaga tattgaaatc ccttcttgaa
     7681 cagggagaga tagactgtgc aataggtatt gtgcgaaatg agaaatggga aacaaaagta
     7741 gtcatgttca ccagccctga ggacataaca aaggccagcg gcacaaaata tacctaccag
     7801 ccggtacttt caagtctcag ggatatacac agggcatttg tggatactta tgttcagggc
     7861 cttaagaaat acaaaaaagt tgcgcttgtg ggagtaccat gccaggccca tggggcaaaa
     7921 cttttccgtg aaaatttcgg cagaatagaa ttgataatag gacttatctg tatggagagt
     7981 ttttccgaac agataatgct aaatgaaatg gttccaaaga tcatgggtgt tgatatcagg
     8041 gacgtcaaga agatgaattt taataaaggt aaattcatcg ttgaaacaga taaagaagta
     8101 aaagaggttc ccattaaagt tgttgcgccc cttgccagga aaggatgtca tcactgccag
     8161 gattatacat catatttt
//
LOCUS       DATGBN010000396         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_537075, whole genome shotgun sequence.
ACCESSION   DATGBN010000396 DATGBN010000000
VERSION     DATGBN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537075"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1178
                     /locus_tag="VKL21_09845"
     CDS             129..1178
                     /locus_tag="VKL21_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HMB46112.1"
                     /translation="MNKIIFKDAKITKPQENQPEQIKKIFPEISPVEKGDIVAIKIHP
                     GEYGNTTHVRPVIIRTVVDLVIKEGGIPFVTDTTTLYKGMKLNAAELIQGAAMNGFTH
                     ASMNAPFIVADGLRGGDGREIMIKGEAIDRITVASGIAQADSMIVISHGKGHPASGFG
                     GAVKHLGMGCLDRAGKIKVHEVGRPSIDPEKCIRCGDCVEICPWGAIDPPGIDHSKCC
                     GELSCADACQQEAIIPPQDASEKMQKRLGEAALGPISALNGRIGYINWVYDLTPGCDC
                     FNFSGERFVEDIGILAGTDPVALDAATIDLINERMKRNGKSIYHVWGVDPLIHLEAVE
                     KIGCGSRKYSLMPWG"
     gene            1229..2464
                     /locus_tag="VKL21_09850"
     CDS             1229..2464
                     /locus_tag="VKL21_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00375 family protein"
                     /protein_id="HMB46113.1"
                     /translation="MQVNADLHIHSKYSMATSPKMDLPTLASESMKKGIQLVATGDCL
                     HPKWLSLIKTLKEENGVFRINANQKTDASFILTTEIEDINQVHHLLIIPSISKAEELY
                     EFMKPHSKDIDSDGRPVVRLDGEQIAQAARSADALIGPAHAFTPWTAMYAYHNSLKEC
                     YGDMAKYIYFLELGLSADTSYADRIEELKCLTYLSNSDAHSPYVNKLAREFNRFEMED
                     ISFDELKMAIIREKGRKPVLNVGMPPDEGKYNESACIRCFRHYTIRESVMKSWKCSCG
                     ARIKKGVKDRVNELASFDKPHYPDHRPPYIHIIPLSEIIAKALGIGPNTKGVQGIWNR
                     LIDKYGSEVAVLIDADISNSGLDTRVISAIIAFREGNVKVHPGGGGQYGRVELPCSDT
                     CHDKAKKNECESQKSLFDF"
     gene            2656..>2964
                     /locus_tag="VKL21_09855"
     CDS             2656..>2964
                     /locus_tag="VKL21_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46114.1"
                     /translation="MIVTKHISIDKECVEKLKPQLEKHNGNFSAAIREIIDRNGKSIF
                     PNNSSAIDSSLFKWLLTEVNGTLIPDNVMDELIDPILINSIRKLEDCLNCRFRELEWD
                     I"
BASE COUNT          982 a          556 c          687 g          739 t
ORIGIN      
        1 gcccatatat attttattaa gcttttacaa attcgtactt cgaaaatatt cattaacatc
       61 gttagcatat gttagcatat tattcattaa tagtcagcaa ataaatgata aaataggatg
      121 tgatggtatt gaataaaata attttcaaag atgccaagat tacaaaaccg caagaaaacc
      181 agccagagca gataaaaaag atctttccgg aaatttcacc cgtagaaaag ggagatattg
      241 tagctataaa aatccatccg ggtgaatacg gcaataccac tcatgtaaga ccggtcataa
      301 taagaacggt tgtagacctt gtaataaaag aagggggcat accttttgtc acagatacga
      361 ccactttata caaaggcatg aagctcaatg ctgcagaact catccagggt gcggcaatga
      421 acggcttcac tcatgcaagc atgaatgctc cctttattgt ggcagatggg ttaagggggg
      481 gcgatggaag agagataatg atcaaaggag aagccattga caggattact gtggcttccg
      541 gtattgccca ggccgattca atgatagtca tctctcacgg gaaaggacac ccggcatccg
      601 gatttggcgg cgccgtcaaa cacctcggaa tgggctgcct tgatagggcc ggaaaaatca
      661 aggttcatga agtgggacgg ccatccatcg atcctgaaaa atgcatccgg tgcggggatt
      721 gcgtggaaat atgtccgtgg ggcgcaatag atccgcccgg aatagatcat tcaaaatgct
      781 gcggcgagtt atcctgtgca gatgcctgcc agcaagaagc catcatcccc cctcaggatg
      841 cctcggaaaa aatgcaaaaa agattgggtg aagcggcatt aggcccgata agcgcactca
      901 atgggcgcat cggttatata aattgggttt atgatcttac tccgggatgc gattgtttta
      961 atttttcagg tgagcgtttt gtggaagaca ttggaatact cgcaggaacc gatccggtgg
     1021 ctcttgatgc tgccacaatt gacctgataa atgaaagaat gaaaagaaat ggaaagtcca
     1081 tataccatgt atggggcgtt gatcccttaa ttcatctgga agccgttgaa aaaatagggt
     1141 gtggcagtcg caaatactca ttaatgccct ggggatgagg ggcattgtgt agcataagca
     1201 ctatatcttt tacagttgat tagcccctat gcaagtaaat gcagaccttc atatccattc
     1261 aaaatacagc atggcaacct ccccgaaaat ggatttgcct accttagctt cagaatccat
     1321 gaaaaaaggc atccagcttg tggctacagg ggattgcctt catccgaagt ggctttcttt
     1381 gatcaagact ttaaaggaag agaacggtgt tttcaggata aatgcaaatc aaaagacgga
     1441 tgcgagtttt atccttacga ctgagatcga agacatcaac caggtgcatc acctcctgat
     1501 aattccttca atttccaaag cagaagaatt gtatgaattc atgaaacccc attcaaaaga
     1561 catagactct gacggcagac ctgttgtcag gttggatggg gaacagatcg cccaggccgc
     1621 aagatcagcc gacgctctta tagggccagc tcatgctttc acgccatgga cagctatgta
     1681 tgcatatcat aattcgctca aagaatgcta tggcgacatg gcaaaatata tttattttct
     1741 agaactggga ttgagcgccg atacatcgta tgcggataga attgaagaac tcaaatgtct
     1801 cacataccta tcaaactcgg atgcgcattc cccctatgtc aataagctcg cccgtgagtt
     1861 caaccgattt gaaatggaag atataagttt tgatgaactg aagatggcaa ttatcaggga
     1921 aaaaggaaga aaacctgttc ttaatgtggg aatgccgcct gatgaaggaa aatacaatga
     1981 gagcgcatgt atccgctgct tcagacatta tacaataagg gaaagcgtca tgaaaagctg
     2041 gaaatgttca tgtggcgcaa ggattaaaaa aggagtaaaa gacagggtaa atgaacttgc
     2101 atcttttgat aaacctcatt atcctgacca ccggccgccg tatattcata tcataccatt
     2161 atctgaaata atcgcaaaag ccctgggcat tggcccaaat acaaaaggcg tccagggaat
     2221 atggaacagg cttatcgata aatacggatc tgaagtagcg gtgctcatag atgcagatat
     2281 ctcgaattca ggactcgaca cacgggtcat tagtgcgata atcgctttca gggaagggaa
     2341 tgtgaaggtt catccggggg gaggaggcca gtatggaagg gttgaattgc catgttcgga
     2401 tacatgtcac gataaagcaa agaaaaacga atgcgaatcc cagaaatcat tgtttgattt
     2461 ctgagaaatg aaaaatatgg atttcgaaac agcaaaatat atagggtaga aaatgaaatt
     2521 atactatgga taggtaaaac cactttatag gttttgtcta gcttaaaaaa atttttttgt
     2581 gcaaaaaggt aaaacggaaa aacggggaag aaaacgttta aatttaaacc ttggagtagt
     2641 aaaaaagttt tggctatgat agttacaaag catatatcca ttgacaaaga atgtgtcgag
     2701 aaattaaaac cgcaactgga aaaacataat ggaaatttca gcgctgcaat aagagagata
     2761 atcgaccgta acgggaaatc tatttttccg aataattcat cggcaataga cagttccctc
     2821 ttcaaatggc tattgacaga ggtgaatgga acgcttattc cggataatgt aatggatgaa
     2881 ttgatagacc caatattgat aaattcaata aggaaactgg aagattgtct taactgcagg
     2941 ttcagggaac ttgaatggga tata
//
LOCUS       DATGBN010000397         8869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_110455, whole genome shotgun sequence.
ACCESSION   DATGBN010000397 DATGBN010000000
VERSION     DATGBN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110455"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VKL21_09860"
     CDS             <1..219
                     /locus_tag="VKL21_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46115.1"
                     /translation="GGGELESFYVSISRPDVPAAIIKRLGTPQFWDSKEDFSKKMAKY
                     YEEISRKAIETAYGEAQQKHTAQKKERI"
     gene            541..1839
                     /gene="aspS"
                     /locus_tag="VKL21_09865"
     CDS             541..1839
                     /gene="aspS"
                     /locus_tag="VKL21_09865"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HMB46116.1"
                     /translation="MIRTHYSNQITPEMNGNSVTVCGWAHEIRDLGGITFLVVRDREG
                     LIQVTLFKKAMDKNVLDVVRNVSRESVVSVTGTVKKEAKAPNGYELVPTAITVLGKAE
                     SPLPMDTTGKVDADLDTRLDVRFMDMRRLRTTSIFRIRHLLLKSIREYLDREGFVEIT
                     TPKVVATATEGGTALFPITYFEREAFLNQSPQLFKQLMMSGGLDRVYEIGPIFRAEEH
                     DTRKHLNEATSIDIEASFIDNEDVMVILENLVNYVYTYISENGKVYLKNLGVEIKIPE
                     LPFKRITYDEAVRIAKDNGEQIEWGDDLSTESEHSIGKNIGEHYFITDWPSKIKPFYA
                     LPYEDKPELCRAFDMMHPRMELSSGAQRVHNHELLRERITAQGLNADGFDFYLKAFKY
                     GMPPHSGWGVGAERLLMTMLGIENIREVVLFPRDRKRLSP"
     gene            1935..2621
                     /locus_tag="VKL21_09870"
     CDS             1935..2621
                     /locus_tag="VKL21_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46117.1"
                     /translation="MSTERDIIIERLEQKLAARDKEFAQMQETLRESIIIEMEKRFSD
                     KIKLRESTLEDMRFKFGEKINEIIQMNKSLRDSIMEKKKTETDILHQCENRMERMEHR
                     LIELNNTYDGVMKELLDQKSIIQELRPPKPKEESRPRPDARPKEEIKPKEVAKPAERP
                     KTQYIIADNYVPREQRKQQPIIEADERPINTEEKNKGNLKEQSKKPTIRKSEKIMEGV
                     EISETLKRGK"
     gene            2621..6133
                     /gene="smc"
                     /locus_tag="VKL21_09875"
     CDS             2621..6133
                     /gene="smc"
                     /locus_tag="VKL21_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMB46118.1"
                     /translation="MHIKEIELQNFKSFGKKVKIPFFDDFTTISGPNGSGKSNIIDSI
                     LFCLGLSSSRVLRAEKLTDLIFNGDSKIKRDFAQVTIHFDNTDREMPVDSDEIVITRK
                     IRRTESGYYSYYYFNEKAVSLNEIHNYLSKAKITPEGYNVVMQGDVTAIIEMTQTERR
                     KIIDEIAGVAEFDEKKDQAINELDIVRERIERVDIILGEVDDRMSKLRQERDQALKYQ
                     SLRDEKRKYEGYILLARQKDARNELDRLGGELQDKETKKIDIIKQIEERRIELLDIEE
                     KLSDLNSLIIRKGESEQIALRKEIESIRGEISRCMSTIELAENEINDIDSQKRKAFLD
                     VDAVQGKIKEFDARHKEETQRNETIKGEVSDKNTQLLILKSKIAEVDARFVETRDSLS
                     AAKLGLETFRNEKNELMREEDRLLDAARRKSTESRDRELEIEDAISKTKSADLDTINA
                     KTGIEEINGKKREISRDLIDLEKNRVQIKSVISDIENTLRTFHQEYAKSEARIRAAAE
                     INYSGPVEAILSARKNKELPGIYGTIAELGKVDKKYSVALEIAAGGRLQSVVVDSDED
                     AARAINYLKERRLGRVTFLPLNKMESMQKMSKTEKEGIIDYAINLVNFERKFDPAFWY
                     VFRDTLVSDNLTTARRLMGNKRLVTLDGELLEKGGAMTGGSIKSRLSFASAEEDNIKK
                     IAEQISEYEGRRKSAVNKLEKLEEHLADIKSESSGFDNEITRLKMQLTEIEGRGSRLS
                     EVIETRNRELKEIEEARIKIKLDMDNVEEQKRRKDSEINTILEEIAKIEVLLKGSQVP
                     ELNNRATHFEEEIQRLTSRIRDIEAGINAITLERKYALEKINETRQRLSELDKKKEEH
                     REKILGLKEKIKSLESDLNLKNSREKELGGELLELQDKRALVQKEHSSLKDRLMDIQR
                     VQIDIDRSLQGLYSTKDALTEQITKLDNEIAGLGIERNEEIPSSESITQRLQALIRAM
                     EKLEPVNMRAIDEYNEVENRQKDLKSRRDILFNEREELLLRIKKYEELKKESFMETFN
                     GINEQFKQVFAELSEGTGALFLEKPDEPFTGGMTIKAQPSEKTLQRLEAMSGGEKSLT
                     ALSLLFAIQQYRPAPFYAFDEIDMFLDGVNAERVAKRIQKSAGNAQFIVVSLRKPMIE
                     AAKRTVGVAMQEFNISSVTGIKLN"
     gene            complement(6157..7020)
                     /locus_tag="VKL21_09880"
     CDS             complement(6157..7020)
                     /locus_tag="VKL21_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMB46119.1"
                     /translation="MRISKHIHAIKLPFQIPIGPGKFVERFEYVYLIYGKKICLIDSG
                     VASSEQKIFDYIRQTGRNPDEISLMILTHSHPDHIGSAPAIKKASGCVVAAHSSEKSW
                     IEDVELQSKERPVPNFHSIVEGSVDVDLILKEKEVLDLGNGLSLEVFHTPGHSKGSIS
                     ILFREDKALFTGDVIPLRGDLPIYEDIFASVKSIKKLKDIRGINLLLASWDGPREGSR
                     VYKLMDESMHYLQQIHTTVINSADIDSSFVSSELCEKVLEKLGLPNISVNPLVAKSFE
                     SSLISRDKTLT"
     gene            complement(7353..7823)
                     /locus_tag="VKL21_09885"
     CDS             complement(7353..7823)
                     /locus_tag="VKL21_09885"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMB46120.1"
                     /translation="MAKNKRQKARYMADGQSYFIILLILSIWLNFIFPIRAILYAPYT
                     YFGIIIICFGIAIGLWSRSLFLKSKTTLSPFESPTSLVTSGPFHISRNPIYLGMTAIL
                     LGIAIFLGTLVTFTFPALFMMIIETLFIPDEERKLEKIFGEQYKEYKNKVRQWI"
     gene            complement(7891..>8869)
                     /locus_tag="VKL21_09890"
     CDS             complement(7891..>8869)
                     /locus_tag="VKL21_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HMB46121.1"
                     /translation="SVIKPLTGDGPSDAAVIRPLLDSMEGVVVANGICPRYGDIDTYH
                     MVACAIDEAVRNHICVGGSFDHLAGLDNFCWCDPEKSEKTPDGEYKLAQLVRANMALY
                     DYTKAYGVPCISGKDSMKNDYQIGGRKISIPPTLLFSTIGKIEDVHKAVTMDVKHPED
                     RVYVLGMTKDELGASEYFASLGYIGNDVPRVNAGSARKLYIALEEAIREGVVASCHDC
                     SDGGLGVALAESAFSGDLGMTIELTKVPVENIKRTDTILFSESQSRFVVTVAPENVKR
                     FEALMKGNVFTDVGVVTPQLNFTVMDGEKVVLSAGIDVLKDAWQKTLRW"
BASE COUNT         2885 a         1784 c         2011 g         2189 t
ORIGIN      
        1 ggtggcgggg aacttgaatc attctatgtc agcatctccc gtccggatgt gcccgctgcg
       61 ataataaagc gcctgggtac gccgcagttc tgggacagta aggaagattt tagtaagaag
      121 atggcaaaat attacgagga aataagcagg aaagcgatcg aaactgctta tggggaggca
      181 caacagaagc acacagctca gaagaaagaa aggatttaat attgaaaaga tgcgccatgg
      241 cttgatgaaa aaattatgga ttattttttt gatatattcg aagaatgaca ggcaggacat
      301 tcgatattat caactggcaa ccctttaaac ttatttccac aatctctaca tatacattcg
      361 gatagtagta tatctctcaa aatatttatt ttatattcgt ctcttgaatt acccattttt
      421 tattccttta attccctctt cggtatagaa cttactgcaa atatgtgtat atttttccat
      481 tgcaggaaac aattcccgat atattaataa tcctctttat cattactagc cgtgatacca
      541 atgataagaa ctcattactc aaaccagata accccggaaa tgaacggcaa cagcgtcact
      601 gtttgcggat gggcgcatga gatacgcgac cttggaggga ttacattcct tgttgtgagg
      661 gacagggaag gactgatcca ggttacgctt tttaagaaag ccatggataa gaacgtgctt
      721 gatgttgtca ggaacgtaag ccgtgaatcg gtggtttctg ttaccgggac tgtcaagaaa
      781 gaagccaaag cgccaaatgg ctatgaactg gttcccacag cgataactgt gcttgggaaa
      841 gcagaatccc cgctgccaat ggataccaca ggcaaagtgg atgcagatct tgacacgagg
      901 cttgatgtca ggttcatgga catgcgccgc ctcaggacaa catctatttt caggattcga
      961 caccttttac tgaaaagcat tcgggaatac ctggacaggg aaggttttgt ggagatcaca
     1021 acgccaaaag tcgtagctac agccacagaa ggtgggactg cgcttttccc gatcacttat
     1081 tttgaacgcg aagcattcct aaaccagagc ccgcaattat tcaagcagct tatgatgtcg
     1141 gggggacttg accgcgtata tgagataggg cccatcttca gagcggaaga acatgataca
     1201 agaaaacatc tgaacgaggc tacatctatt gatatcgaag caagtttcat tgacaatgaa
     1261 gatgtaatgg tgatactgga gaatcttgta aattatgttt acacatatat ttcagagaac
     1321 ggcaaggtat atttgaaaaa ccttggagtt gaaataaaaa tccccgagct tcctttcaaa
     1381 cgaattacct atgatgaagc cgtcaggata gcaaaagata acggggaaca gatcgaatgg
     1441 ggcgacgacc tttctaccga atccgaacac tcgatcggga aaaatatcgg cgagcattat
     1501 ttcatcactg actggccttc aaagataaaa ccattctatg ctcttcctta tgaagataaa
     1561 ccggaattat gcagggcatt tgacatgatg catccgcgaa tggaactatc ttcaggtgcg
     1621 cagcgcgttc ataatcatga attgttaaga gagaggataa ctgcccaggg actaaatgca
     1681 gatggttttg atttctatct caaggcattc aaatacggta tgccgccaca ttccggctgg
     1741 ggagttggag cagaacgcct gctaatgact atgcttggaa tagaaaatat ccgggaggtc
     1801 gtattattcc cgcgtgacag aaagcgatta tccccataaa ttatttaact ctaaagtatg
     1861 tttatacagc gaagagtcaa gatttaccaa aaactatttg actattttaa ccgttaagag
     1921 aacgagggat atgcatgagc actgaaaggg atattataat tgagagactt gagcaaaaat
     1981 tggctgcaag ggacaaagaa tttgcacaaa tgcaggaaac tttacgggaa tccattatca
     2041 ttgaaatgga gaaacgtttt tctgataaga taaagcttcg ggaatccaca cttgaagaca
     2101 tgagatttaa attcggggaa aagatcaatg agataatcca gatgaataaa tccctcaggg
     2161 attcgatcat ggagaaaaag aagactgaga ccgatattct gcaccagtgc gaaaaccgta
     2221 tggaaaggat ggaacacagg cttatcgagc tcaacaacac ttatgatgga gtcatgaaag
     2281 agcttcttga tcagaaatcg ataatacagg aattaagacc ccctaaaccc aaggaagagt
     2341 caaggccaag accggatgca aggccaaaag aagagataaa gcctaaggaa gttgcaaagc
     2401 ctgctgaaag gccaaagacc cagtatatca ttgctgataa ttatgttccc agggaacaga
     2461 gaaaacaaca gcctataatt gaagcagatg aaagacccat caatacagaa gagaaaaaca
     2521 aagggaactt aaaagaacaa tccaaaaaac caacaataag aaaaagtgaa aagataatgg
     2581 aaggggtgga aataagcgag accttgaaaa gaggtaaata gtgcatatca aagagataga
     2641 acttcaaaat ttcaaatcct ttggaaaaaa agtaaaaata cccttctttg atgatttcac
     2701 aacgatttcc ggccctaacg gaagcggcaa atccaatata atcgatagca tccttttttg
     2761 tcttggtctc tcaagttcca gggtattgcg cgctgaaaaa ctcactgatc ttattttcaa
     2821 tggggattcc aaaataaaaa gggattttgc ccaggtcacg atccattttg acaacactga
     2881 tagggaaatg ccagtggact cagatgagat cgtgataacc cgcaaaataa gacggacaga
     2941 atccggttat tacagttact actatttcaa tgaaaaagct gttagcttaa atgaaatcca
     3001 taattacctt tcaaaagcca agataacccc ggaaggttac aatgtggtca tgcaggggga
     3061 tgtcacagct atcatagaaa tgacccagac cgaaagacga aaaataatag atgagatcgc
     3121 aggcgttgct gagttcgatg agaaaaaaga ccaggccata aatgaactgg atattgtccg
     3181 tgaaagaata gaacgggtag atatcatcct tggtgaagtg gatgaccgga tgagcaagtt
     3241 gaggcaggaa agagaccagg ctttaaaata ccagtcgctt cgtgatgaaa aaagaaaata
     3301 tgaaggctat attcttctgg caaggcagaa ggatgccagg aatgaactgg atagactcgg
     3361 cggggaatta caggataaag agacaaaaaa aatcgacatc ataaaacaaa ttgaagagcg
     3421 cagaattgaa ctccttgata tcgaagaaaa actttcggat ctaaattcct taattatccg
     3481 caaaggcgaa agcgaacaga tagcccttcg aaaagagatc gaatccataa ggggtgagat
     3541 ctcccgctgc atgagcacaa ttgaacttgc agaaaacgag atcaatgata tcgattccca
     3601 gaaaaggaaa gctttccttg atgtggatgc tgtccagggc aaaataaaag aatttgatgc
     3661 caggcacaaa gaagaaaccc agcgtaatga aactattaaa ggagaggtca gcgataaaaa
     3721 tacccaattg ctgatattaa agagcaagat cgcagaagtt gatgcaaggt ttgtggaaac
     3781 ccgtgacagt ctttcagccg caaagcttgg tcttgagacc ttcaggaacg aaaagaatga
     3841 actgatgcgc gaggaagacc gacttcttga tgctgcgcga aggaagtcaa cagaaagcag
     3901 ggacagggaa ctggagatag aggatgcgat atccaaaacg aaatctgcag atcttgatac
     3961 cataaatgca aagaccggga tcgaagaaat aaatggaaaa aagcgtgaaa tttcacgcga
     4021 tctgatagac cttgagaaaa accgggtgca gatcaagagc gtgatctccg atatcgagaa
     4081 tacgcttcgc acattccacc aggagtatgc aaagtcagag gccaggataa gagccgcggc
     4141 agagatcaat tacagcgggc ctgtagaggc gatccttagc gcgcgaaaga acaaggaatt
     4201 accgggtatc tacggcacga tagcagaact gggcaaagta gataagaaat attccgttgc
     4261 ccttgagata gcagccggag gtcgtttgca gtctgtggtc gttgattctg atgaggatgc
     4321 cgcaagggca ataaattacc tgaaagaacg ccgtcttggc agggtgacat tcctgcccct
     4381 taacaagatg gaaagcatgc aaaaaatgtc aaagaccgaa aaagaaggta tcatcgatta
     4441 cgccattaat cttgtgaatt ttgaaaggaa gtttgacccg gcgttctggt atgttttccg
     4501 ggataccctg gtttcagaca atctcacaac tgcaaggcgg ctcatgggaa ataagcgcct
     4561 ggtaacgctt gatggtgaac tcctggaaaa aggcggggca atgacagggg gttcgatcaa
     4621 atcaaggctc tcatttgcca gtgctgaaga agataatatc aagaaaattg cggaacagat
     4681 atcagaatac gagggcaggc gcaaatcggc tgtaaataaa cttgaaaaac tcgaggaaca
     4741 ccttgctgat atcaagtcag aatcttccgg ttttgataat gagatcacaa ggctaaaaat
     4801 gcaattaact gaaatcgagg ggcgtgggtc gcgtctatct gaagttattg aaacaaggaa
     4861 cagggaactt aaggagatag aggaagcccg cattaagata aagctggata tggacaatgt
     4921 cgaagagcaa aaacgccgga aagacagcga aataaatact atactggaag aaatcgcaaa
     4981 gatcgaagta ttgttgaaag gctcgcaggt tcccgaactt aataaccggg caacacattt
     5041 cgaagaagag atccagaggc taacgagcag gatccgggat attgaggcag ggatcaatgc
     5101 gatcacactt gagcgcaaat atgccctgga aaaaatcaat gaaacaagac agaggctgtc
     5161 tgaacttgat aaaaagaaag aagaacaccg ggagaagatc cttggcttga aagagaagat
     5221 caaatctctt gaatccgact tgaatcttaa gaattcaagg gaaaaagaat tgggcgggga
     5281 acttctggaa cttcaggata aaagggcgct ggtacagaaa gaacattcct ctttaaagga
     5341 caggcttatg gatatccaga gggtgcaaat agatatcgac aggagtttgc agggattata
     5401 ttccacaaaa gatgccctga ccgaacagat aacaaagctt gataatgaga tagcagggct
     5461 tggaattgaa cgaaacgaag agatcccgtc atcagaatcg atcacccaga gattgcaggc
     5521 gcttatcagg gcaatggaga aacttgaacc tgtgaatatg agggccattg atgaatataa
     5581 cgaggtggag aacaggcaaa aagaccttaa atcccggcgc gacatactgt tcaatgaaag
     5641 ggaagagctg ctgctgcgta tcaagaaata tgaagaactg aaaaaagaat ccttcatgga
     5701 gactttcaat ggcataaacg agcaattcaa gcaggtcttt gctgaattat ccgaagggac
     5761 aggcgcgctt ttccttgaaa aacccgatga gccattcacg gggggaatga ccataaaagc
     5821 ccagccatcc gaaaagacat tacagcgcct tgaagccatg tcgggcggtg agaagagcct
     5881 caccgcatta tcgctccttt tcgctatcca gcagtacagg cccgcgccgt tctatgcttt
     5941 tgatgagata gatatgttcc ttgacggggt aaatgccgaa agggtcgcca aacgcatcca
     6001 gaaatccgcc ggaaatgccc agttcatcgt ggtctcactg agaaaaccca tgatagaggc
     6061 ggccaaacgc accgtcgggg ttgcaatgca ggagttcaat atatcgagtg tgacggggat
     6121 caagttgaat tgatcttaat tgcttaaatt caattgtcat gtgagagttt tgtcacggga
     6181 tattaagctg gattcaaaag attttgcaac gagtggattt acagaaatat tgggtaaccc
     6241 taacttttcc aggactttct cacataattc agatgacacg aatgaagaat caatatcagc
     6301 actattgatg acagtcgtat gaatttgttg aagataatgc atactttcat ccatcagctt
     6361 atatactcgg cttccttccc gggggccatc ccaggaagcc agtaataaat tgattccgcg
     6421 tatatccttt agttttttaa tagacttaac agacgcaaaa atatcttcat aaatgggcaa
     6481 atctcctctt agtggaatta catcccctgt gaatagcgcc ttatcctcgc gaaacaggat
     6541 ggatatagac ccttttgaat gtcctggtgt gtggaaaact tccaggctta gaccgtttcc
     6601 taaatccagt acttcttttt ctttaagaat tagatccaca tcaactgatc cctcaacaat
     6661 tgaatgaaag tttggaaccg gtcgttcctt tgactggagt tcaacatcct ctatccagga
     6721 tttttcactt gaatgtgcag cgaccacaca accacttgct tttttaattg ccggcgccga
     6781 accaatatga tccggatggg aatgtgttag aatcatcaaa gagatctcat caggatttcg
     6841 ccccgtctgc ctgatataat caaagatctt ttgctctgaa gaagcaacgc cactatcaat
     6901 gagacaaatc ttcttaccat agatcaaata gacatactcg aacctttcga caaatttccc
     6961 tggaccaatt ggtatctgga aagggagttt tattgcgtga atatgtttgg aaattctcat
     7021 tttctatcta taaaattatt tcattccata aaacgtcaga agaaagagta aaattgctaa
     7081 gacttggttt tgacagtagg gaaatagaaa ggctttatgt taaatataac aagtgcaaaa
     7141 tggtctacac tctccatatt atcgaaataa tagaaatcga tctcctccgg aacagtaaaa
     7201 tctgcataaa tgacgaatta tgcacaatgg cttaattaat tgcacgtttc tgcggatgac
     7261 accgatgtgc ggatctgtga aaatccgtcc gctggagtcc cgattccgcc tcatacgctt
     7321 ggaaaagact tcgtttccta ggaaatgagg agttatatcc actgtcgtac tttattttta
     7381 tattctttat actgttctcc aaatattttt tctagcttcc tttcttcatc tggaataaat
     7441 aaagtttcaa ttatcatcat gaatagggcc gggaatgtaa aagtaaccag tgttcctaga
     7501 aatatagcga tacctaatag tatggctgtc attccaagat atattggatt tcggcttata
     7561 tgaaaaggac cggaagtcac gagtgacgta ggcgattcaa aaggtgatag tgttgtttta
     7621 ctctttaaaa ataatgagcg gctccagagg cctatggcta ttccgaagca gatgattatt
     7681 atcccgaaat acgtgtatgg agcataaagg atcgcccgga tagggaatat gaaatttaac
     7741 cagatggaca ggattagaag gattatgaaa taagattgtc catctgccat atatcttgcc
     7801 ttttgtcttt tgtttttagc catattgtag attaaatccc atgacgctaa tatttgttat
     7861 cccgttctct ccagattgta aaatttccaa tcaccacctc aacgtcttct gccatgcatc
     7921 cttaagcaca tcgatccccg cgctcaggac caccttttcg ccatccatga ctgtgaaatt
     7981 taactgcggt gtaacaacac caacatccgt gaacacattc cccttcatga gcgcttcgaa
     8041 tcttttaaca ttttccggcg ccacggtgac tacgaacctg ctctgggatt ctgaaaataa
     8101 tatcgtatcc gtgcgcttaa tattctcaac agggactttt gtcagttcga tggtcatacc
     8161 aagatctcct gaaaatgcgc tctcggcaag agctacccca agaccgccgt cagagcagtc
     8221 atggcaggag gccaccactc cttcccggat ggcttcttca agtgcgatat acaattttct
     8281 ggctgaacct gcattcaccc tgggaacatc attcccgata tatccaagag aggcaaaata
     8341 ttccgaagct ccaagttcgt cttttgtcat tccaagaacg taaaccctat cttcaggatg
     8401 cttgacgtcc attgtcactg ctttatgcac atcctctatt ttgcctatgg ttgaaaaaag
     8461 aagagttgga gggatcgata tcttcctgcc gccgatctgg taatcgttct tcatgctgtc
     8521 ctttccggaa atgcagggga cgccatacgc ttttgtataa tcgtataaag ccatgttggc
     8581 tcttaccagc tgggcgagct tatattcacc atccggagtt ttttctgatt tctcaggatc
     8641 gcaccagcag aaattatcaa gaccggcaag atgatcgaag gagccgccca cacagatatg
     8701 gttccttacg gcttcatcga tggcgcaggc taccatgtgg taagtatcta tatccccgta
     8761 tctgggacat attccattag cgactaccac gccttccatg ctgtcaagca gcggccggat
     8821 aactgcagca tcactggggc cgtctcctgt gagcggttta atgacagag
//
LOCUS       DATGBN010000398         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_304357, whole genome shotgun sequence.
ACCESSION   DATGBN010000398 DATGBN010000000
VERSION     DATGBN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304357"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..651
                     /locus_tag="VKL21_09895"
     CDS             247..651
                     /locus_tag="VKL21_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HMB46122.1"
                     /translation="MNKKNRLLFGVAIVVVLLGYLFFSSGTSKVYEVSEAVAARETFD
                     GKPPITVNGTLIPGTDNWNPLTNTLTFKMTDGIATMDVVYIGDKPNMAAEAVSIQAIV
                     TGQFKNNVFEAFRMLTKCPSKYDGGPVATQGK"
     gene            898..3513
                     /gene="mutS"
                     /locus_tag="VKL21_09900"
     CDS             898..3513
                     /gene="mutS"
                     /locus_tag="VKL21_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMB46123.1"
                     /translation="MDKMTPAMKQYYEAKEKHNDALIFFRMGDFYESFGDDAKVIAKE
                     LEITLTSRGRDKEGQDMPLAGIPHHAIDSYLPKLIKKGYKVAICEQLEDPKKAKGVVK
                     RGVVRVITPGTVIDSSLISDQSNNYLMAVSGEGTQFGVSFLDVSTGEFLTTQFSDNSS
                     YDKIISEASRMHPAECILAPALFENMKLTQRLRQSGFVLNKFDNEAFNTKNAREQLLK
                     HFGIMTLEGLGCEKLDLAIAASGAALVYAKTTQMRDLNHIRTMRTYSENEFMVLDSIT
                     LRNLEIIQNIRGEGARSLLSVIDRTKTPMGGRLIRKWLLLPLISVSKITERLDSIDEI
                     VKKTLLRYDLRSQLSRIRDIERLIGRIVYGNSNARDLIALKISLLSIPEITASLKENG
                     VKSGLLPGISERLGDHKDIVELIGRAFVDDPPVSVREGGMVKEGYNEKLDELKKMSLH
                     GKDWIAEMQQKERERTGIKSLKIGYNSVFGYYIEVTKTNLSQVPSDYIRKQTTANGER
                     FYTPGLKEKEVMILSADEKRTALEFELFTEINYFIAGRSKELQITAQALGELDVITNL
                     AQMAVDNKYIRPEINDGCNVLIRDGRHPVVEFAIPGGFVPNDTHMDCQDNQFLLLTGP
                     NMAGKSTYMRQTALIVIMAQIGSFVPASFASIGLVDRIFTRVGAFDDLASGQSTFMIE
                     MVELANILNNSTPRSLILLDEIGRGTSTYDGYSIAKAVVEFIHNKERVGVRSLFATHY
                     HQLTAISSVLKRVKNYHVAVKEEGNDLVFLRKIIPGATDKSYGIHVARLAGVPLKVTQ
                     RAKEILKEMENESSIGSRGKDSARYTQLMLFDTDNAAKESPVVGELKKLDVDAMTPLE
                     ALNALAGLKKKASQE"
     gene            complement(3557..4033)
                     /locus_tag="VKL21_09905"
     CDS             complement(3557..4033)
                     /locus_tag="VKL21_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMB46124.1"
                     /translation="MVNNKKILITYASGTGSTEKLAQVIAEGIRKVEGIAVDVKRARD
                     VKPDDVVSANGYAFGSHSATEYMEGEIKNLFEELYLRKEKISGKPVLLFTTGQGRQVA
                     ALASLEKFASLLNPQFIKPGLAVKGMPGEADKANAIEMGEKLADAVNKFKSNYIVA"
BASE COUNT         1361 a          882 c          950 g         1136 t
ORIGIN      
        1 tggttatcat gttcaataaa agcgccatag ggattgccgc aagaagtgga atgcccatcc
       61 agaaaaaaag aggtgtgtaa atgatcgccc ctccaagccc cagtatggag aaaaggacag
      121 cgatcatgaa tgcaattaca atgacaggga ttatatccat atttgatcat aaacacatcg
      181 aaggattaat aatgcttgga agaaacattt aagtttaaga gaaacattta gttcgtaagg
      241 atacaaatga ataaaaaaaa tcgtttatta tttggtgttg cgatcgttgt tgttttatta
      301 ggatacctct ttttttcctc aggaacctcg aaagtatatg aggtcagtga agcggtagcg
      361 gcgcgggaaa cttttgacgg aaaacctcca attaccgtta atggcacact aatacccgga
      421 actgacaact ggaacccgtt aactaatacc ctcacattta aaatgaccga cggcattgca
      481 acaatggatg tcgtatatat tggcgacaaa cccaacatgg cagcagaggc ggtaagtatc
      541 caggccatag ttacagggca attcaagaac aatgtatttg aagccttcag gatgctgacc
      601 aaatgtcctt caaaatatga tggcggtcct gttgctacac agggcaaatg aagatttgat
      661 tttctttttt tttttaggat ttgtatatag ttggtcatca tgcctataca caggtttcat
      721 gaaacattaa tggataggtg aggaaccaaa aaatagtttt ttggatagga taggaggttc
      781 ctctaatgct aaatacatgt gctacattta aatcaatttt ccatgattat acccaaatat
      841 ttaccaattc atccaaaaaa caaaaatagg aataaatgaa tatcacatcc ggaacccatg
      901 gacaagatga cacctgcgat gaagcagtat tacgaggcaa aggaaaagca taatgatgca
      961 ctcatttttt tcaggatggg tgatttctat gagtcttttg gcgatgatgc aaaagtaatc
     1021 gcgaaggaac ttgaaataac actcacatca agaggcaggg ataaagaagg ccaggatatg
     1081 cctctggccg ggataccaca tcatgctatt gattcatatc ttccaaaact tataaagaag
     1141 ggatataagg tagcaatatg cgagcagctt gaagacccga aaaaagcaaa aggagttgta
     1201 aaaagaggag ttgtcagggt catcactccc ggaactgtca ttgactcatc tcttatttcc
     1261 gaccaatcca ataattacct tatggcagtt tccggtgaag gtacgcaatt tggggtatcg
     1321 tttcttgatg ttagtacggg cgaattcctg accacccagt tttcagataa tagttcatac
     1381 gataaaataa taagcgaagc ttcccggatg catcctgctg agtgcatact tgctcccgcg
     1441 ctttttgaaa atatgaaatt gacccagaga ttgaggcaat ccggtttcgt tcttaacaaa
     1501 ttcgacaatg aagcttttaa cacaaaaaac gcacgtgagc agcttctaaa acattttggg
     1561 ataatgaccc ttgaaggttt aggatgcgag aaactcgatc ttgcgatcgc agcttccgga
     1621 gctgcccttg tttatgcaaa aacaacccag atgagggact tgaatcacat ccggacaatg
     1681 agaacttatt ccgaaaatga gttcatggta cttgattcca taacgctgag gaatcttgag
     1741 atcatacaga acatccgggg cgagggggca cgatccttat tatctgtcat tgaccgcaca
     1801 aaaacaccca tggggggacg tctgatcagg aaatggctgc ttttgccctt gatctctgtt
     1861 agtaaaatta cagaaagact ggattccatt gatgaaattg tcaaaaaaac ccttttgcgg
     1921 tatgatctgc gctcccagct ctcgcgaata cgagacatag aacgacttat cggtcgtatc
     1981 gtatatggca actcgaatgc ccgtgattta atagcgctta aaatttcact tctttcaata
     2041 cctgagatca cagcttccct taaagaaaac ggtgtaaaat cagggctttt gccgggtata
     2101 tcagaaaggc tgggcgatca taaggatata gtagagctga ttggaagggc ctttgttgat
     2161 gacccgcccg ttagcgttcg cgaaggcggc atggtaaagg aaggatataa tgaaaagctt
     2221 gatgaactca agaaaatgtc ccttcatgga aaagactgga tcgctgaaat gcagcagaaa
     2281 gaacgagaaa ggacaggtat aaaatccctt aagataggat acaattccgt attcggatat
     2341 tacatcgagg tcacaaaaac aaacctctca caagttcctt cagattatat aagaaaacaa
     2401 acaactgcaa acggcgaacg cttttacacc cctggactca aggaaaaaga agtgatgata
     2461 ctttctgccg atgaaaaacg aacggcgctt gaatttgaac tcttcaccga gatcaattat
     2521 ttcattgcag gaaggtcgaa agaattgcag atcacggcgc aggctctcgg agaactggat
     2581 gtcatcacga accttgcgca aatggccgtg gataataaat acataagacc cgaaataaat
     2641 gatggctgca atgttctgat aagggatgga aggcatcctg tggtagaatt tgctattcca
     2701 ggcggctttg tcccgaatga tacacacatg gattgccagg ataaccagtt cctgctcctg
     2761 acagggccaa acatggctgg aaaatccaca tacatgaggc agacggcgct aatagttata
     2821 atggcacaaa ttggcagttt tgtgcccgcg tctttcgctt ctatcgggct tgttgaccgg
     2881 atattcaccc gcgtaggggc atttgatgac ctcgccagcg ggcagagcac ttttatgata
     2941 gagatggtgg aacttgcaaa tatccttaat aattccacac ccaggagcct tattctgctt
     3001 gatgagatcg gaaggggcac aagcacctat gatggttaca gcatagcaaa agccgttgtt
     3061 gaattcatcc ataacaaaga acgtgtgggt gtgaggtcgc tttttgcaac ccattatcac
     3121 cagctaactg ccataagcag tgtcctgaaa cgggtgaaaa attatcacgt ggccgttaaa
     3181 gaagaaggca atgaccttgt attccttcgc aaaatcattc ccggcgctac agataagagt
     3241 tacggtatcc atgtagcaag gcttgcaggc gtccctttga aggtaacgca gagagcaaag
     3301 gagatcttaa aagagatgga gaacgaaagt tccatcggca gcaggggaaa agacagcgcc
     3361 aggtatacac agctcatgct ttttgatacg gataatgctg caaaagaatc gccggtcgtt
     3421 ggggaactca agaaacttga tgtggacgca atgacgccgc ttgaggcgct gaatgcgctg
     3481 gcaggactca agaagaaggc atcgcaagag tagtggtcgc agaattcaag aaatatgttc
     3541 attcatggga attatttcac gcgacaatat agttggattt gaatttattt accgcatctg
     3601 cgagtttttc tcccatttca atagcatttg ctttatctgc ttctcccggc atacctttaa
     3661 ccgccagacc tggtttgatg aactggggat ttaataagct tgcaaatttt tccaatgatg
     3721 caagcgcagc aacctgcctt ccctgtcctg ttgtaaaaag taatacaggt ttgccactta
     3781 ttttttcttt tcgtagatag agttcctcaa agagattttt aatttcccct tccatatact
     3841 cggtagcaga atgtgagcca aaagcataac cattggctga aaccacatca tctggtttga
     3901 cgtcccttgc cctcttcaca tcaactgcta taccctccac ttttctaatc ccttctgcga
     3961 ttacctgcgc aagtttttca gtactccctg ttccagaggc ataagttatt agaatttttt
     4021 tattattaac cacaagatca tctcctgtta tgattatatc tggaataatg atacaggaac
     4081 tataaacact ttttcttcga ctccgcacta tgcaaaaagc tcagcaggga agaatgatat
     4141 gcctggagag atagataaga aaagggagat gaattaatga gttccattgg aaaagaattt
     4201 atggaaaaaa caaagttcca gtatcttgac aggtctgacc aatcccgtaa cttaccccag
     4261 cctccattag agatggaata tgatgcatca agaccagtaa gggatcttcc cggacctgaa
     4321 aatatcaaa
//
LOCUS       DATGBN010000399         6095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_304432, whole genome shotgun sequence.
ACCESSION   DATGBN010000399 DATGBN010000000
VERSION     DATGBN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304432"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VKL21_09910"
     CDS             <1..292
                     /locus_tag="VKL21_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HMB46125.1"
                     /translation="IGFNLGSLIKLKGDARIREMNYMRTNIKHARKYNLEMILTSDLD
                     SVYDLRSPREMAALSSLFGMTQSEAVDAMSSNPIGILRRKSSNYIQEGIEIL"
     gene            289..651
                     /locus_tag="VKL21_09915"
     CDS             289..651
                     /locus_tag="VKL21_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HMB46126.1"
                     /translation="MKTLPTLREKKRYIAFEINSEKAIFRQELVREISNSIISLFGDI
                     GASQIRSALMSFEGRFGILRCAREKTVETRAGLACINNIRGIRVSIRVLGISGTIKGA
                     TEKFIQQSLIKESEPERR"
     gene            656..1429
                     /gene="psmA"
                     /locus_tag="VKL21_09920"
     CDS             656..1429
                     /gene="psmA"
                     /locus_tag="VKL21_09920"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HMB46127.1"
                     /translation="MQMAPQMGYDRAITVFSPDGRLFQVEYAREAVKRGTTAVGVKAV
                     DGVALLVDKRVTSKLLEAESIEKIFQIDDHIGAASSGLVADARALIDRARVEAQINKV
                     TYDEPIGVEVLSKKICDFKQSYTQYGGVRPFGTALLIAGVDDHKARLFETDPSGALLE
                     YKATSIGSGRTETMEVFEKKYTDDIKLEDAIILGLDALHSTTEGVFNASTIEVGIIEL
                     STMKFRKLTPSEVEKYVERVIKMHASETKGKDKKKKSEE"
     gene            1437..2132
                     /locus_tag="VKL21_09925"
     CDS             1437..2132
                     /locus_tag="VKL21_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HMB46128.1"
                     /translation="MVTLDESIIARLKTHGKTFEVFVEPEGALALKKGDSVKIENILA
                     VEDVFLDAKNGDRPAEQDVINAFGTTDALQIAEKIVMDGELHLTTEQKKKIQDEKKHR
                     VISIIATNAINPQTKGPHPPARIEAAINEAGVHIDPMKNVDELVEIAMKAIRPLIPIR
                     FEEVKIAIKFPAQFATKAYGSVAGFGTLTKQEWQNDGSWIGVITIPAGKQDELYSLLN
                     RLTKGSAETKFLK"
     gene            2138..2974
                     /gene="rrp4"
                     /locus_tag="VKL21_09930"
     CDS             2138..2974
                     /gene="rrp4"
                     /locus_tag="VKL21_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HMB46129.1"
                     /translation="MEKKLVIPGDFLSDDVKLADEGTYIEDGKVFSSVFGIASLKNQI
                     KVVPLSGKYIPRQGDLVIGIITEVSFSNWIVDIRSPYEGLLHISEFPRRIDNSEMSKY
                     LKVGDSIMTLVKDVDQSMKVELTLNDQRLRLIKDGRVIEVTPSKVPRLIGRSGSMIAM
                     LKNETNCNIFIGQNGRIWLTGKDKNLDLAARTIQKIEKESHIPGLTDRIVKFLKEEKG
                     IPVKEPVKKKEEKEAAKKLKEEVAQETVNDETVTEETEEAEPEKVEENESGGILDELL
                     DK"
     gene            3019..3750
                     /gene="rrp41"
                     /locus_tag="VKL21_09935"
     CDS             3019..3750
                     /gene="rrp41"
                     /locus_tag="VKL21_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HMB46130.1"
                     /translation="MVKPEKLIENGIRLDGRKPDEIRPIKIKVGVLNRADGSCYFEFG
                     GNKVIAAVYGPREVHPRHMQKSNSAVVRYRYNMASFSVEDRKRPGPDRRSIEVSKVSR
                     EALEPVIFQEYFPRSAIDIFVEVLQADAGTRTAGINAASIALADAGIPMKSLVSACAV
                     GKVDDTLVLDLNKDEDNYGQADMPIAMTPDGNITLLQMDGHLTREEFKEGLEMAKKGC
                     LMIAELQRAALLEKYSSLMEGEVNE"
     gene            3734..4522
                     /gene="rrp42"
                     /locus_tag="VKL21_09940"
     CDS             3734..4522
                     /gene="rrp42"
                     /locus_tag="VKL21_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HMB46131.1"
                     /translation="MKSMSNEIMSELRKDYIYNLIIKGEREDGRKFDEYREITIDTNV
                     IEKAEGSARVKIGDTHLVVGVKIQTGTPFPDTPDQGVIITNLELIPLASPVFESGPPR
                     EDAIELARVVDRGIRESESIDLSKLCITPGEKVWMVFIDVHVLDDGGNIMDAASLGAI
                     AALMTSKLPAQRFDVGEDTPVPMRDVPVAVTAVEIGGTIMLDPSLHEQSIAGTKLTVI
                     SNQDGGISGMQKSGVHPLTTEQINYIVDISIEKAKELREKFLEI"
     gene            4525..4836
                     /locus_tag="VKL21_09945"
     CDS             4525..4836
                     /locus_tag="VKL21_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HMB46132.1"
                     /translation="MAKISSKGHKSRSAGRFGVRYGTKNRKLIADIEEKMHAEYTCSK
                     CGAKKISRIGTGIWQCKKCEHTFAGGSYVPQSPAHMTVLRAVQGVKAEISKQLAQGIK
                     A"
     gene            4849..4986
                     /locus_tag="VKL21_09950"
     CDS             4849..4986
                     /locus_tag="VKL21_09950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HMB46133.1"
                     /translation="MVYKCTRCKRAVEIDYEYSGVRCPYCGHRILVKERPVLVKQVKA
                     E"
     gene            4983..5411
                     /locus_tag="VKL21_09955"
     CDS             4983..5411
                     /locus_tag="VKL21_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46134.1"
                     /translation="MIVTTARKPSSKTRIFCKHLGRFTGWRYVTRGKTSLLEFADEPF
                     LLVGEYKGNPGSFSFFLNGKCVLSIRANVSLDRDIGIGNEPVIQGNNSVALNMSKITG
                     LGTGEKSKRVILVNDNIEFTDDGESYIKLKVLEVRGEGSV"
     gene            5404..>6095
                     /locus_tag="VKL21_09960"
     CDS             5404..>6095
                     /locus_tag="VKL21_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46135.1"
                     /translation="MFDLFKKEEINSLKERISRLEEENRILSLQLGKKDDKAKKTIAI
                     KQDVDRELNEARNKISSLTNEIQTLKNETTQEFKFRLSESLSKNRLEEIIFSLGGFQA
                     KISTLITVYLEKDKALVDVAGDTVNLFDSSALHLIEKIESSTGKIIFYDTNRIVNLVI
                     IPVLPILRSECVLNRQFDLQPLKENLEYDKILVVNAHAGETIIGIVEADTFVEHEIVR
                     SNVMGKHKQGGW"
BASE COUNT         2057 a         1116 c         1490 g         1432 t
ORIGIN      
        1 tatcggattc aaccttggct cacttataaa acttaagggg gatgcgcgaa taagggaaat
       61 gaattatatg agaaccaata tcaaacatgc caggaaatac aatctcgaaa tgatcctcac
      121 aagtgatctc gattcggttt atgaccttcg ttccccgaga gaaatggcgg cgctttcaag
      181 ccttttcggg atgacacaat ccgaagctgt tgatgcaatg agctctaatc ctatcggcat
      241 ccttcgaagg aaaagctcca attatatcca ggaaggcatt gaaatattat gaaaaccctg
      301 ccaaccttac gcgaaaaaaa gaggtatatc gcgtttgaaa ttaattcaga gaaggcgatt
      361 ttccggcagg aacttgtcag ggagatctca aattcaataa tatctttatt cggtgatatc
      421 ggagcaagcc agataagatc cgcgctaatg tcctttgagg ggagattcgg aatacttcgc
      481 tgcgcccggg aaaaaacagt tgaaacaagg gcaggtcttg cctgtatcaa taatatcagg
      541 ggaatacgcg tttctatccg ggtactgggc atatccggca ctatcaaagg tgcaacggaa
      601 aagtttatac aacaatcact cattaaagag tcagaaccgg aaaggagatg ataaaatgca
      661 aatggcacca caaatgggat atgacagggc aataactgtt ttcagtcctg atggaagatt
      721 gtttcaggtc gaatatgcgc gtgaagcagt taaacgcgga acaacggcag ttggggtgaa
      781 agccgtggat ggggtggcac tattggttga taagcgtgtt accagtaaat tacttgaagc
      841 ggaatcaatt gaaaagatat tccagataga tgatcatatc ggagctgcct catcaggtct
      901 tgtcgctgat gcaagggcgc taatagaccg cgcccgcgtt gaagcccaga taaacaaagt
      961 aacttatgat gaacctatag gtgttgaggt cctctccaag aaaatctgcg acttcaagca
     1021 atcatataca caatatggtg gcgtaagacc ttttggaaca gctcttctta tcgctggagt
     1081 tgatgatcac aaggccaggc tttttgaaac cgacccgagc ggcgctctcc ttgaatataa
     1141 ggccacaagc atcggttcgg gaaggaccga gaccatggag gtcttcgaga aaaaatatac
     1201 cgatgatatc aaattggagg atgcaatcat tctcgggctg gatgcgcttc acagcacaac
     1261 agaaggagtt tttaacgctt caactatcga agtcgggata attgaacttt caaccatgaa
     1321 attcaggaaa ctcacacctt cggaagtgga gaaatacgtt gaacgggtta taaagatgca
     1381 tgcctctgaa accaagggaa aggacaagaa aaagaaatca gaagagtgag tgaaatatgg
     1441 ttactctaga tgagtccata atagcgcgcc ttaagaccca cgggaaaact tttgaggttt
     1501 ttgtagaacc ggagggagcg ctggccttaa aaaagggcga ttccgtaaag atcgagaata
     1561 tcctggcagt tgaggatgtg ttcctggatg ccaaaaatgg cgacaggcct gctgaacagg
     1621 acgtaattaa cgcattcgga acaaccgatg cattgcagat cgcagaaaaa atcgtcatgg
     1681 acggagagct tcatcttaca actgaacaga aaaagaagat acaggacgaa aaaaagcata
     1741 gggtaataag catcattgcg accaacgcca tcaatcccca gacaaagggg ccgcatccac
     1801 ccgcacggat cgaagccgct attaatgaag ccggggtgca tatcgatcct atgaaaaatg
     1861 tggatgaact tgtagagatc gcaatgaagg ctataaggcc cctcataccg atacgttttg
     1921 aggaggtaaa gatagctatc aagtttccgg cgcaattcgc tacaaaagcc tatggcagtg
     1981 ttgcaggatt tggaaccctg acaaagcagg aatggcagaa tgacggttcc tggataggtg
     2041 ttataaccat tccggcggga aagcaggatg aactgtacag cctcctgaac cggctaacaa
     2101 aagggagtgc ggagacaaag ttcttaaagt gatgtaaatg gaaaaaaaat tagtaattcc
     2161 gggtgatttc ttatcagatg atgtaaagct tgctgatgag ggtacatata ttgaagatgg
     2221 taaagtattt tcatctgtct ttggaatagc atcattgaaa aaccagatca aagtcgtgcc
     2281 gctttcaggc aaatatatac ccaggcaggg cgaccttgtt atcggtataa taaccgaagt
     2341 ctctttttcg aactggatag tggatattcg ttccccgtat gagggtttac tgcatatatc
     2401 cgaattccca agaagaatag acaactcgga aatgtcaaaa taccttaaag tcggcgattc
     2461 aataatgacg ctcgttaagg atgttgacca gagcatgaag gttgaactta cgttgaatga
     2521 ccagagactg aggctgataa aagacggacg ggtaatcgaa gttacgccat ccaaggtgcc
     2581 aagattaata ggaagaagcg gctctatgat cgctatgctt aagaatgaga caaattgcaa
     2641 tatattcatc gggcagaacg gaaggatatg gcttacaggc aaagataaaa atctcgatct
     2701 tgcagcaagg acgatccaga aaattgagaa agaatcccat attccgggtt tgactgacag
     2761 gatagtcaag ttcttaaaag aagaaaaagg gataccagta aaagaaccgg taaaaaagaa
     2821 agaggaaaaa gaagcggcaa agaagctgaa agaggaagtg gcccaggaaa cggtcaatga
     2881 cgaaacagta accgaagaaa cggaagaagc agaacctgag aaggttgagg agaatgaatc
     2941 cggtggaatc ctggatgaac tgctggataa ataatttgta tgaaattatg aggaaagagg
     3001 aagaaagaga ggaattttat ggtaaaacca gaaaagctaa ttgaaaacgg tattcgcctt
     3061 gatggcagaa aaccagatga aatcagaccc atcaaaataa aagtgggcgt tttgaacaga
     3121 gctgacggtt catgttattt tgaattcgga ggaaacaagg taattgcagc agtatatggt
     3181 ccgcgagagg tacatccccg ccatatgcag aaatcaaaca gcgcagttgt acggtataga
     3241 tataatatgg catcgttctc agttgaagat cgaaaacgcc cggggcctga caggagaagc
     3301 atagaagtat caaaggttag cagggaagcg ctggaaccgg tgatattcca ggaatatttc
     3361 ccccgttccg ccatagatat atttgtagaa gtgctccagg cagacgcggg tacaaggacg
     3421 gcaggaatta acgcagcatc aattgctctg gcagatgcag gcattccaat gaaaagcctg
     3481 gtatcggcat gtgctgttgg aaaagtcgat gatacgctgg ttcttgacct gaataaagat
     3541 gaagataact atgggcaggc agatatgcca atagccatga cgcctgatgg aaatatcacg
     3601 ctattgcaga tggatggtca tcttaccagg gaagaattca aggaagggct tgagatggca
     3661 aagaaaggat gtcttatgat cgctgaattg caaagagccg cacttctgga aaaatattcc
     3721 tcccttatgg aaggtgaagt caatgagtaa cgaaataatg tccgaactcc gtaaagatta
     3781 tatttataat ctcattataa aaggggagag agaggatggg cgcaaatttg acgagtacag
     3841 ggagatcaca attgacacaa atgttattga aaaggcggaa ggctccgcac gcgtcaaaat
     3901 cggggatacg catcttgtcg taggtgttaa gatccagacc ggtacacctt tccctgatac
     3961 acctgaccag ggagtgatca tcacaaacct ggaacttatt ccccttgctt cgcctgtgtt
     4021 tgaatcaggt ccaccgaggg aagatgccat tgaacttgcc agggtagtgg acaggggcat
     4081 ccgtgaatcc gaatctattg acctttccaa gctgtgtata acaccaggcg aaaaagtctg
     4141 gatggttttc attgatgtgc atgtacttga cgatggtgga aacattatgg atgcggcttc
     4201 tctgggggcc atagcagctc ttatgacctc aaaattgcct gcacaaaggt ttgatgtggg
     4261 agaagatacg ccggttccaa tgagggatgt tcctgtggct gtaacggcgg tcgagatagg
     4321 ggggaccata atgcttgatc cctcactgca tgaacaaagc atagccggaa ctaaattgac
     4381 cgtaatttcc aatcaggatg gcggaatatc aggcatgcaa aaaagcggtg ttcatccact
     4441 tacgaccgaa cagataaatt atatagtgga tatttcaatt gagaaggcaa aagaattaag
     4501 agaaaaattc ctggagatat aatcatggcg aaaatcagtt caaaaggaca taaatcaaga
     4561 tcggccggaa gatttggtgt aagatatgga accaaaaacc gaaaacttat tgcagatatt
     4621 gaagaaaaaa tgcatgccga atatacatgt tcaaaatgcg gcgcaaagaa aatttcaagg
     4681 atcgggacag gtatatggca atgcaagaaa tgtgaacaca cttttgcagg aggctcatat
     4741 gttcctcaaa gtccggcgca catgacagta ttaagagcag tccagggtgt aaaagccgaa
     4801 atttccaaac agctggcaca aggaattaaa gcataataag gattcagtat ggtttataaa
     4861 tgtacccgtt gcaagcgggc agtggagata gattatgaat atagcggcgt ccgttgccct
     4921 tattgcggcc accggatcct ggtaaaggaa agacctgtac ttgttaaaca ggtcaaagct
     4981 gaatgatcgt taccactgca cgaaaaccat catcaaaaac acgaatattc tgtaaacatt
     5041 taggcaggtt cacaggctgg aggtatgtga cccgcggaaa aacaagcctt ttagagtttg
     5101 cagatgaacc ttttttattg gtaggagaat acaaaggaaa ccctggcagc tttagttttt
     5161 ttttaaatgg taaatgtgtt ctctcgattc gcgccaatgt atcactcgat agagatatcg
     5221 gcatcgggaa tgaacctgta attcaaggaa ataattctgt cgcgcttaat atgagtaaaa
     5281 taacaggatt aggaacagga gaaaaatcaa aaagggttat cctggttaat gataatatcg
     5341 aatttactga tgatggtgaa tcctatatta aattaaaagt actggaagta cgaggcgagg
     5401 gcagtgtttg atttatttaa gaaagaagag atcaattccc tgaaagaaag aatatcccgg
     5461 cttgaagaag aaaatagaat actctccctg cagcttggaa aaaaagatga taaagcaaaa
     5521 aaaacgattg caataaaaca ggatgtagac agggaattaa atgaggccag aaataagata
     5581 tcttcactta ccaatgagat acaaacactg aaaaatgaaa caacacagga gtttaaattc
     5641 aggctttctg aaagtttatc aaaaaacagg cttgaagaga ttattttctc tcttggtgga
     5701 tttcaggcca aaatatcaac attgataacc gtttacctgg aaaaagataa ggcgcttgta
     5761 gatgtggcag gagatacagt taaccttttt gacagttcag ccctgcatct tatcgaaaaa
     5821 atcgaatcat ccacaggaaa aataattttt tacgatacta accggatagt taatctggta
     5881 ataataccgg ttcttccaat tcttcgatca gaatgcgtcc tgaacaggca attcgatctt
     5941 caaccattaa aagaaaatct tgaatatgac aaaatacttg ttgtgaatgc ccatgcaggt
     6001 gaaaccatca taggaattgt tgaagcggat acttttgtgg agcatgaaat cgtaagaagc
     6061 aatgttatgg ggaagcataa acagggaggc tggag
//
LOCUS       DATGBN010000400         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_576412, whole genome shotgun sequence.
ACCESSION   DATGBN010000400 DATGBN010000000
VERSION     DATGBN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576412"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..370
                     /locus_tag="VKL21_09965"
     CDS             134..370
                     /locus_tag="VKL21_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46136.1"
                     /translation="MTELTPAEIEVRRTSVQFSRRTARAINSRLFLSYTATHLSKIMN
                     TLMALGKYGTFEQLPDWLQQAHYPVRISEYGRGI"
     gene            367..612
                     /locus_tag="VKL21_09970"
     CDS             367..612
                     /locus_tag="VKL21_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46137.1"
                     /translation="MKKYQVMNHENGQTNAYVAMKKIYWNNMSDKKIIPVKLTPIIQK
                     AINIASEKHLGQKRKADGLPYIVHPFAVAWLLSNIQI"
     gene            563..1057
                     /locus_tag="VKL21_09975"
     CDS             563..1057
                     /locus_tag="VKL21_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMB46138.1"
                     /translation="MCIPSQWHGYYPIYRYDEEVIAAGLLHDTLEDVASYRFDDLKND
                     FGERLASIVKEVSEDKDPNIITDEKATWEDRKRKYLKHLKITSWEAMLVCVADKIHNL
                     SSLLDAYKEQGDTAWSKFNSTPENILWFNGEVLQILRTRMDGELVRELERVYAQVCEK
                     TGFS"
     gene            complement(1074..1295)
                     /locus_tag="VKL21_09980"
     CDS             complement(1074..1295)
                     /locus_tag="VKL21_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46139.1"
                     /translation="MSLSSALNPDGGYFMLCFSEHEPGSYGPRRVTQAEIRATFGKGW
                     KINYIREAVFETMFGAKGVKAWLSSITRL"
     gene            complement(1317..1544)
                     /locus_tag="VKL21_09985"
     CDS             complement(1317..1544)
                     /locus_tag="VKL21_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46140.1"
                     /translation="MNILVHARSPTSASSPATVSDLNDRTTVDHRAYAPQTRSLRTGT
                     NHLTSCPPTSAPAPLHRQRAAGRLCCGNHRA"
     gene            complement(1718..1885)
                     /locus_tag="VKL21_09990"
     CDS             complement(1718..1885)
                     /locus_tag="VKL21_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46141.1"
                     /translation="MKPKKYEAKDEDDLLKLIDEDPDKFQGWEGPVEANLRSVHYTTK
                     KTKRKPRLANK"
     gene            complement(1891..2160)
                     /locus_tag="VKL21_09995"
     CDS             complement(1891..2160)
                     /locus_tag="VKL21_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HMB46142.1"
                     /translation="MVNWKNLDLEFDEHAFKHGIKDYEIEQIFKGRIYKRKIYFNKEL
                     RYQIIGKAFGRLIMVIVVPLGNNKLKVFSARMASEYKGIYNDKAK"
BASE COUNT          707 a          454 c          506 g          665 t
ORIGIN      
        1 cagtaatctc aaagaagctc ttggtgaaaa tgagtttgaa aggctcataa aagaagtaga
       61 atcagtagca gaggagatta taaggaatat gctggaatga gaaaaagaag atggctgatg
      121 tgaggacaaa aatatgacag aattaacacc cgccgaaata gaagtaagaa gaacgtcagt
      181 acaattcagc aggcgcaccg caagagccat taactcccgg ttattcttga gttatacagc
      241 gactcacctc tctaagatca tgaatacttt aatggctctg ggtaaatatg gcacattcga
      301 acagctcccc gattggttac agcaagccca ttatccagtc agaatctccg agtacgggcg
      361 cgggatatga agaagtatca agtgatgaat catgaaaatg gacaaacaaa tgcttacgtt
      421 gctatgaaaa agatatactg gaataatatg agcgacaaaa agatcatacc cgtcaaattg
      481 actccaataa ttcaaaaagc cattaacata gcttctgaaa aacatttagg acaaaagcgc
      541 aaggcagatg gattgccgta tattgtgcat cccttcgcag tggcatggtt attatccaat
      601 atacagatat gatgaagaag ttatcgctgc cggtctgctg catgatactt tagaggatgt
      661 ggcaagctat cgttttgatg atcttaagaa cgactttggt gaaagactgg caagtatagt
      721 caaggaagtt tcagaagata aagacccaaa tatcataaca gatgaaaagg ctacatggga
      781 agacaggaag agaaaatacc tgaaacatct gaaaatcacc agctgggaag caatgcttgt
      841 gtgtgttgct gataagattc acaatttatc ttcattgctg gatgcgtaca aagaacaggg
      901 cgacactgca tggagtaaat tcaattcaac tcccgaaaat atactgtggt ttaatggcga
      961 ggttttgcaa attttgcgga caagaatgga tggagaatta gttcgagagt tggagagggt
     1021 ttatgcacaa gtttgtgaaa agactggttt ttcttgataa tgtccgacgg tgactataga
     1081 cgagttattg aggatagcca ggctttaaca ccctttgcac caaacattgt ttcaaaaacc
     1141 gcttccctga tgtaattaat cttccaacct ttgccgaaag tagcccgtat ctcagcctgg
     1201 gttactctcc ttggcccata tgatcccggc tcatgctcgc tgaaacagag catgaaatac
     1261 ccgccatcag gattgagcgc agacgataaa ctcacaacaa aaattgggcg ctcctatcag
     1321 gcacggtgat ttccacaaca tagccttcct gctgctcttt gcctgtgcag tggcgctggt
     1381 gctgaggttg gcgggcatga ggttaggtga ttcgttcctg ttcgcaggga tcgggtttgg
     1441 ggcgcatatg cacgatgatc aacggttgtc ctgtcattga gatcgctaac ggtggccggg
     1501 gaagacgcag aggtggggga tcgggcatga acaagtatat tcataatttt tagagaatag
     1561 ccaataagga tgtgccggtt gcaggcgaaa tcggcggtgg attaatataa ggagggaggc
     1621 tgagatatat ttaataggga ggtatgggta tcacattgaa cttattttca tatagttcaa
     1681 cttcgcaaaa atctgatgcc acctcctttc caaaaattta tttatttgct aaacggggtt
     1741 ttcttttagt tttctttgtt gtataatgga cagatctcaa atttgcctct acagggcctt
     1801 cccacccttg gaatttatcc ggatcctcat caataagctt aagaagatcg tcttcatctt
     1861 ttgcttcata tttcttgggt ttcataattt tcatttcgct ttgtcattat atatcccttt
     1921 gtattcggaa gccattcttg cactaaaaac ctttagtttg ttatttccta aaggtactac
     1981 tataaccatt ataagcctac caaaagcctt gccaattatc tgataacgca attctttatt
     2041 gaaataaatc tttcttttat aaatcctgcc tttaaagatt tgttctattt cataatcttt
     2101 aatcccatgt ttgaaagcat gctcatcaaa ttctaagtcc aggttcttcc aattaaccat
     2161 aaattagact ctcaatgcat gggatattta aaattgttat ggcgagatat tttttcagcg
     2221 cctccgcgat ctccatgcaa tctcataaat gcaattcgtc gctatcatca ggtttcttta
     2281 tcgccccggc gcccaccatg tccgtaaata aggacttggg atagcagtca gg
//
LOCUS       DATGBN010000401         7315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_304550, whole genome shotgun sequence.
ACCESSION   DATGBN010000401 DATGBN010000000
VERSION     DATGBN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304550"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..706)
                     /locus_tag="VKL21_10000"
     CDS             complement(77..706)
                     /locus_tag="VKL21_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HMB46143.1"
                     /translation="MDAVVMAGGMGKRLGKDEKPLTPLYGKPLIQYVLEALLGSRKIE
                     RIFVATSPRVKRTNEWMDDFKKAHDKVEIIHTRGEGFVHDMIAAVEEAGIRESVFIIM
                     ADLPLVTSGLIDRIIKKYNEVNTPALSVHMKLDVFTRLGLRPDTVFHKNSDFIVPCGI
                     NILDAGSIHREQEDYNLILEDEELAINVNAPGDLIVCERFIQNKFNYPI"
     gene            complement(691..1461)
                     /gene="cobS"
                     /locus_tag="VKL21_10005"
     CDS             complement(691..1461)
                     /gene="cobS"
                     /locus_tag="VKL21_10005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HMB46144.1"
                     /translation="MRKFILALRNGFGFLSTIPVGITMEGIENLMKHIYLFPVVGTVI
                     GIILALIVIALTSLTVLPPIMVSLFVIILIYYITGFNHIDGLADFGDGLAAHGTKDKK
                     IAAMRDTAIGAGGIVFCMIALIGLFVSLVSIQESEIYLLLPFALIVAETSAKQSMVTV
                     AAFSKSLHAGFGAMTVNNTKRSDLILGTVFSAAVCYVVLGVLGIEAFIVSQLAGLIVL
                     NTANRHFGGVSGDVVGASNEIGRLAALLYIGGMGWMLL"
     gene            complement(1458..2069)
                     /locus_tag="VKL21_10010"
     CDS             complement(1458..2069)
                     /locus_tag="VKL21_10010"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HMB46145.1"
                     /translation="MKLSNIKNEKKKKNGDVDNSYTIGIMKVLEEADLPEETLVLAAM
                     ELYVPHPGVETKEIAQAVFKRELKHALSDPNLCILLYAGMLLEKAGEKGELPGMNKET
                     FNKDLTFLIVDEVIGMSIAKYISGDKGIFEYVRFDKLKPGILSKLGPFMDDVIAGLIG
                     GASANMYSRGKDYGAKHEKKKAKKTTARKASNKSKIIAGGFAG"
     gene            complement(2084..3001)
                     /locus_tag="VKL21_10015"
     CDS             complement(2084..3001)
                     /locus_tag="VKL21_10015"
                     /inference="COORDINATES: protein motif:HMM:NF002281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HMB46146.1"
                     /translation="MMEFWSKVLLLAVLFDIIFGEMPAYFHPVVWMGKLISLFVGAAP
                     CRYRKLYGLSMVIFCVGITVLLALLLEFIGTGILGLAICAYFLKSSFSIRMLLISAIG
                     IQKDLDSGMIEKVRSDLKTFVGRNTSGLNSYQSTSAVIESLAESFVDSVLSPLFYFLL
                     LGLPGALSYRMINTLDSMVGYKKEPFLELGYSAAKLDDIVNWLPARISIIFIFITSIF
                     SGKPFDSIRTCIQDHNKTASPNSGWAMAAVSGAMNVRLEKAGYHVLGEKYQPPETSHI
                     RKTVFIIVFSSLLVIGVIFFIGKIPLVPL"
     gene            complement(2998..4407)
                     /gene="cobD"
                     /locus_tag="VKL21_10020"
     CDS             complement(2998..4407)
                     /gene="cobD"
                     /locus_tag="VKL21_10020"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HMB46147.1"
                     /translation="MHLNNRVRKNVFDAVPCIHGARLIESAHDVGKTTNDLLDFSVNL
                     NPLGPPKLKSVLSASYKNIGNYPDNRYLDFRKAAADYLKVSPETIVPGNGSSELIRLF
                     AEAVIEPGEKIIIPAPTFAEYEFQCRLSGAYIEKVDYEEILNINIDNCKAVFLCNPNN
                     PTGKLLNRKEVLSLAEKCSSSEVFLFVDEAFIELADPKESIADIAACNDFVIVLRSLT
                     KTFAVPGLRIGFAVASPVLAGLLNNIRLHWNMNSIAAFVGEKLLKDNQDYINRSLGLL
                     KKERDWLTSKLAGIRGFRPYSSDTNFILVDIRDFGLNSAELTERMLRHGIIIRDCSSF
                     NLENHIRVAVRRRNENRALIGAFSSVITEWGGELAEKEIGKALVRGTATRSRIDCEYY
                     PCHFAGQDCTFCFCPFYPCENIKTGGELIHRSTGGTVWSCAGCNLIHQEQIADKVLKE
                     LMGARKIQDVWKRVVEPVL"
     gene            4742..5032
                     /locus_tag="VKL21_10025"
     CDS             4742..5032
                     /locus_tag="VKL21_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-histone chromosomal MC1 family protein"
                     /protein_id="HMB46148.1"
                     /translation="MAEIRNFVLREKNGNETGVFTGHQPRQAALKAANRSKGTKSKPV
                     ELRLRERGTKKIHIYKGWTEVVAAPKNKPKWMPAKINKPNVKKVGVELLDKV"
     gene            complement(5064..5630)
                     /locus_tag="VKL21_10030"
     CDS             complement(5064..5630)
                     /locus_tag="VKL21_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HMB46149.1"
                     /translation="MKQKVNGSTMNIKLTLLSIMILLFAGCITKEDDDMEKISVMVEG
                     FNEGEMIPDEFTCNGKDISPLIYWKEIPAGTKSITLIMDDPDAPGGTFVHWVIYNMPA
                     QTRNLPKGMPVDKTLVDGSMQGMTDFGKPGYGGPCPPPGKPHRYFFKVYALDMKLNLP
                     VGASKNQVESAMKGHILADGEMMGKYQR"
     gene            complement(5698..5772)
                     /locus_tag="VKL21_10035"
     tRNA            complement(5698..5772)
                     /locus_tag="VKL21_10035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5734..5736),aa:Pro,seq:tgg)
     gene            6200..>7315
                     /locus_tag="VKL21_10040"
     CDS             6200..>7315
                     /locus_tag="VKL21_10040"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMB46150.1"
                     /translation="MAILPGIAPIVASAATPDIYVNQTGWWRADGAFNASATRIQAAI
                     NNATTSDTIYVYNGSYTENVNVNKQVTLQGEGAGVVTIIAALTSDHVVNVTVSYVNIS
                     GLKVTGATGYNKAGIYLGSGVDHANISDNNASNNYYAIYLNSSSNNNLTNNSANSNNN
                     DGIKLNSSSNNNTLTNNIAILNGVTGISLLSSSNNNTLTNNTANSNLIGIGLMTSSKN
                     NTLTNNTANLNTVRGIYLSSSSNNNTVTNNTANSNINIGILLDSSSNNTIYNNYFNNT
                     NNAQDDGYNVWNTTKTAGTDIIGGSWLGGNYWSDYAGVDTDSDGLGDTLTPYNSSTNI
                     TNGGDYHPLNVTPSTIYVNTTGWWYDGGSFNANGTTPI"
BASE COUNT         2194 a         1689 c         1393 g         2039 t
ORIGIN      
        1 taacaaaatt cgatgacagc ttaaagagac atttaaaaga gataaatgaa aagagaaaaa
       61 aggatgagga tttgagttat attggataat taaatttatt ttgtatgaac ctctcgcaaa
      121 caatcaggtc cccgggtgca ttgacattga tcgccagttc ttcatcttca aggatcaggt
      181 tataatcctc ctgctccctg tgaatacttc ctgcatcaag gatgtttatc ccgcacggga
      241 cgatgaaatc actattctta tgaaaaacag tatccggtct tagaccaagc ctcgtgaata
      301 catcaagttt catgtggacc gaaagtgcag gagtgttcac ctcattatat tttttgatta
      361 tcctgtctat aagtccgctc gtgacaagag gaagatcggc cataataata aaaacactct
      421 ccctgatgcc tgcttcttca actgctgcga tcatgtcatg aacaaaacct tcgcccctgg
      481 tatgaataat ttcaacttta tcatgggctt tcttaaaatc atccatccat tcattggttc
      541 ttttcacacg cggtgatgta gccacaaaaa tgcgttcgat tttccttgaa ccaagaagcg
      601 cttcgagcac atattgtatc aatggtttac cgtaaagggg agtaagaggt ttttcatctt
      661 tacccaggcg ctttcccatg cctccggcca ttacaacagc atccatccca tacctccgat
      721 ataaagcagg gctgcaaggc gtccgatctc atttgacgcg cccacaacat ctccgctcac
      781 acctccaaaa tggcggtttg ccgtattaag aacaataagt cctgccaatt gggacactat
      841 gaacgcttca ataccgagta ccccaagaac aacataacat actgctgccg aaaaaacagt
      901 tccaagaatt agatcggacc gctttgtatt attaacggtc attgccccga aacccgcatg
      961 gaggctcttg ctaaaagcag ccacagtgac catggattgc ttggcgctcg tttctgccac
     1021 tatcaatgca aagggaagaa gtaaataaat ctctgattcc tggatggaaa caagagatac
     1081 gaagaggcca ataagcgcta tcatgcaaaa aacaatgccg ccggcgccaa tagctgtatc
     1141 acgcatggca gcgatctttt tatcttttgt tccatgcgcg gccaaaccgt cgccaaaatc
     1201 agcaagcccg tcaatatgat tgaatcctgt aatataatat atcaatataa tgacaaacag
     1261 tgagaccatt attggaggca ataccgttaa tgaagttaat gctatcacaa ttaatgcaag
     1321 aataatacca ataacggtac ccactaccgg gaatagatat atgtgcttca taaggttttc
     1381 aatcccctcc atggttatac ccaccggtat tgtagaaaga aaaccaaaac catttcgaag
     1441 ggctaatatg aactttctca tccggcgaaa ccgcccgcta ttattttgga tttattcgat
     1501 gcttttcttg ctgttgtttt ttttgctttc tttttctcgt gttttgcgcc gtaatccttc
     1561 ccccttgaat acatattcgc ggatgctccg cctatgagcc ctgcaatgac atcatccatg
     1621 aaaggcccga gttttgacaa tatgccgggt tttaatttgt caaatctcac gtattcaaag
     1681 atccctttgt caccactgat gtattttgcg atactcatcc cgatcacttc atccacaatc
     1741 aggaatgtca ggtctttatt gaaagtctct ttattcatac ccggaagctc ccctttctct
     1801 ccggcttttt caagaagcat gcctgcatat agtaaaatac acaggttcgg gtcggaaagc
     1861 gcatgcttta attccctctt aaaaacagcc tgcgcgattt cctttgtttc aactccggga
     1921 tgaggcacat aaagttccat agcggccaaa accagcgttt cctccgggag atccgcttct
     1981 tcaaggactt tcattatacc tattgtatag gaattgtcaa catctccgtt ctttttcttt
     2041 ttttcatttt tgatattcga taatttcata attttctcca tttttaaaga ggaactaagg
     2101 gtatcttccc tataaagaat attaccccga tcaccaataa tgatgaaaaa actattatga
     2161 aaaccgtttt tcgaatgtgt gaagtctctg gtggttgata tttctcaccc aatacatggt
     2221 atccggcttt ctcaagcctc acattcattg cgcctgaaac agcagccatc gcccatcctg
     2281 aatttggtga tgcggtctta ttatggtcct ggatacatgt tctaattgaa tcgaaaggct
     2341 tgccagagaa gatcgatgta ataaatatga atataatgga gatcctcgct ggtagccagt
     2401 tgacaatatc atcaagtttt gcagccgaat atccaagttc caaaaaaggc tctttcttat
     2461 aacccaccat ggaatcaagc gtatttatca tgcggtaaga aagggcgccc ggaagaccca
     2521 acaataaaaa atagaataac ggtgataaga cactgtctac aaagctctct gccagggatt
     2581 caataactgc tgatgttgat tggtacgaat tgagccccga cgtattcctg cccacaaatg
     2641 tttttaagtc gcttcttact ttttcgatca ttcctgaatc caggtctttt tgtatcccta
     2701 tggcagaaat aagcagcatt cgaatggaaa agctggattt taaaaaatat gcacagattg
     2761 ccaatcccaa aattcctgtt ccgataaatt caagcaaaag tgccagcaaa accgttatcc
     2821 ctacacagaa aatcaccatg gacaaaccat ataatttcct gtatcggcaa ggtgcagcgc
     2881 ccacgaacag gctgatcagt tttcccatcc ataccacagg atgaaaatat gcaggcatct
     2941 ccccgaatat gatatcaaaa agaactgcaa ggagtaaaac cttactccag aactccatca
     3001 taaaacaggc tccaccacac gtttccagac atcttgtatc ttcctggcgc ccatcaactc
     3061 ttttagcacc ttatctgcaa tttgctcctg gtggataagg ttgcagccgg cacagctcca
     3121 gactgttcca cctgttgatc tgtgaattag ttctcctccc gtttttatat tttcacatgg
     3181 gtagaaggga cagaaacaga atgtgcaatc ctgtcctgca aagtggcacg ggtaatattc
     3241 gcaatcgatc ctgctccggg tggcagttcc ccgaacaagc gctttaccta tctctttttc
     3301 tgcaagttcg cctccccatt ccgtgataac gcttgaaaag gccccgatca acgcgcggtt
     3361 ctcatttctc ctgcgcacag ccacacggat gtgattttcc agattaaagg aagagcaatc
     3421 acgaatgatt ataccatgtc ttaacatgcg ctctgttaat tcagctgagt tcaagccgaa
     3481 atctcttata tcgacaagga taaaattcgt atcgcttgag tatggtctaa agccccgtat
     3541 tccggcaagt tttgatgtca accaatcccg ttccttcttt aataatccaa gcgacctgtt
     3601 gatataatct tgattatcct taagaagctt ttcacctaca aatgcagcaa tggaattcat
     3661 gttccagtga aggcggatat tatttaacag ccctgccaga acgggtgatg caacagcaaa
     3721 accgatcctt aatcccggaa ctgcaaatgt ttttgtaagg gaacgcaaaa cgataacaaa
     3781 atcgttacac gctgcaatgt ctgcaatact ttctttggga tcggcaagtt caatgaaggc
     3841 ctcatctaca aataagaata cctccgatga ggagcatttt tcagcaagcg aaaggacctc
     3901 ttttcgattc aaaagcttcc cggtaggatt gttgggatta catagaaaaa cggccttaca
     3961 attatctata ttgatgttta aaatctcttc ataatccact ttttcaatat acgcacctga
     4021 gagccggcac tggaattcat actctgcaaa tgttggggcg ggaattatga tcttctctcc
     4081 cggttcaatg accgcttctg caaaaagccg gatcaattct gatgagccat ttccagggac
     4141 tattgtttcg ggagacacct taagataatc agctgcagct tttctaaaat caaggtatct
     4201 gttatcggga taatttccta tgttcttgta agaagcgctc aataccgatt tcaattttgg
     4261 cggtccgaga ggattaaggt ttacactgaa atcgagcaga tcgttcgtag ttttccccac
     4321 atcatgggcg ctttcaatta atcttgcgcc gtgaatgcat gggactgcgt caaacacatt
     4381 tttacgtact ctgttattaa ggtgcacatg cccttttttg tttgcaggga atattaaagt
     4441 gctgtaagga aaatccttca aaaaagatgc acaatataaa tttttatcat taacaccacc
     4501 tatacatact gtttatatta cattaagata atcgaactgt tcgaagacat ttgatttttt
     4561 gtcttagtgt tatattttta tgccaactgg ctcggttttt tcaacgtttg aaataccaat
     4621 atgcgtagat tcattttctt aaaacaatag gctttgccat acttttcaca acaacattta
     4681 tatactatga tgtctgattc tactacaaga caacaaaacg ttgtcggggt atgaatcaaa
     4741 tatggcagaa ataagaaatt ttgtattaag ggaaaagaat ggaaatgaaa ccggagtatt
     4801 taccggtcac cagccgcgcc aggcagcatt gaaagcagcc aatcgcagca aaggaacaaa
     4861 atcaaaaccc gttgaattga gattaagaga gagaggaaca aagaagattc atatatacaa
     4921 gggctggaca gaagtagttg cggctccaaa gaacaaacca aaatggatgc ctgcaaagat
     4981 caacaagcct aatgtaaaga aagtaggcgt tgagctgctg gataaagtct aatttttaat
     5041 tttaatattt ttctttttta agtttatctc tggtattttc ccatcatctc accatctgca
     5101 agtatatgcc ctttcatggc gctttccacc tggttctttg aagctcccac tggcaggttg
     5161 agtttcatat caagagcata aactttaaaa aaatacctgt gaggttttcc agggggggga
     5221 catggcccac catatccggg tttcccaaaa tcagtcatac cctgcatact tccatcaaca
     5281 agggtcttgt ccacaggcat gccttttggc agattccggg tctgagctgg catgttatag
     5341 atcacccagt gaacgaaagt ccctcccggg gcatcggggt catccattat caatgtgatg
     5401 ctctttgttc ctgcaggtat ctctttccag taaattaaag gtgaaatatc tttgccatta
     5461 cacgtaaatt catcaggtat catttctccc tcattaaatc cctctaccat gaccgaaatt
     5521 ttctccatat cgtcatcctc ctttgtgatg catcctgcaa aaagcagtat cattatactc
     5581 aataatgtga gcttgatatt cattgtgctc ccattaactt tctgtttcat tcaatattat
     5641 agttttcgag cccgtgacat cctccccagg cgtttcaaaa tctgcgcaaa acgcaaatgg
     5701 gatagcgcgg atttgaacct tggtctaagc gtcccaaacg ctcaaggatg gagcagccta
     5761 ccctaccatc ccgtggggcg tctcaaaaca gtccttataa gataaaaacc ttaccgatat
     5821 ttcatcaata gattgctgca ttagcgattc cgtttattct tggggggtca tgataaaata
     5881 aataattagt ttagctataa tagaaagata tatacatcat aattatcatc aatatgatat
     5941 tactatatta tagtaaaatc acggggaaaa acggggggta ttacttgtat tgcaagtaaa
     6001 atgaaatgag tcaaaaaata tatttaatag gagcaactga aaatatatgg gtcagcatat
     6061 taagaaatgg aatgtatcga tcacttttgt aatcacgata attatattat tgacaatgaa
     6121 aatctctgtt ttctcatcga tcagagagca ttgtaaaaag ataatacaaa gaaaaaggat
     6181 tctcataata ttttttatta tggcgatctt acctggcatc gcaccaattg tcgcatcggc
     6241 tgcaactcca gacatctacg ttaaccaaac cggctggtgg cgcgcagacg gcgcgttcaa
     6301 cgcaagcgcc acgcggatac aggcagcaat caataacgcc actaccagcg atacgatcta
     6361 tgtgtacaac ggcagctaca ccgagaatgt gaacgtgaac aagcaggtta cgctgcaagg
     6421 cgagggcgca ggtgtggtga ctataattgc ggctttgact tctgaccatg ttgttaatgt
     6481 gactgtgagc tatgtgaaca tctcagggtt aaaggtgacc ggagcaactg gatataataa
     6541 ggctgggatt tatcttggca gtggtgtgga tcacgctaac atttctgata acaacgcatc
     6601 gaacaactac tacgccatct atctgaactc ttccagcaac aacaacctca cgaataactc
     6661 tgcaaattcg aacaataacg acggcatcaa actgaattct tccagcaaca acaacacgct
     6721 cacgaacaac atcgccattt tgaatggcgt caccggcatc tctctgctct cttcgagcaa
     6781 caacaacacg ctcacgaaca acacggcaaa ttcgaacctt attggtatcg gtctgatgac
     6841 ttcgagcaaa aacaacacgc tcacgaacaa caccgccaat ttgaacaccg tgcgcggcat
     6901 ctacctgtcc tcttccagca acaacaacac ggtcacgaac aacaccgcca attcgaacat
     6961 caacatcggc atcctcctgg attcttcgag caacaacact atttacaaca attacttcaa
     7021 caacacaaac aacgcacagg acgatggata taatgtctgg aacactacaa aaacagcagg
     7081 cactgacatc ataggcggtt catggcttgg cgggaactac tggtcagact atgcgggagt
     7141 tgatacggac agcgacggtc tgggcgatac ccttacgccg tacaactcat cgaccaatat
     7201 tacaaacggc ggcgactatc atccgctgaa tgtaacaccg tcaaccatct acgtcaatac
     7261 aactggctgg tggtatgacg gcggcagctt caatgcaaat ggcacaacgc ccata
//
LOCUS       DATGBN010000402         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_71430, whole genome shotgun sequence.
ACCESSION   DATGBN010000402 DATGBN010000000
VERSION     DATGBN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71430"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..974
                     /locus_tag="VKL21_10045"
     CDS             78..974
                     /locus_tag="VKL21_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46151.1"
                     /translation="MDQQLAYKLLAGFLVFTMLGSVFAYFFIGGRDDTTQDPQTPDTN
                     LDKYNPEFWTVNQPFNSISDALSMTPVDAEEAYFVDLENMPIKMLEWTRSGNRITSGL
                     IQEMDTKIYKSNTTKLYYALLKGGNNETSPPFLLLSTMSPPKNDFDYYVIPNTSNILQ
                     RQDTGAINIMGTPVIYAPTDKIAGDVIDIIYSLNKSVTAYDQYEKLLNKVEPVAYQMV
                     SSNVSFARQFYVGIGLNNGSYERNTVYLNANTSVLMKLNQSRANSTERGFQQYIVNQS
                     GNYTTVKIVSPELLTVLSEETS"
     gene            1068..1439
                     /gene="rpl7ae"
                     /locus_tag="VKL21_10050"
     CDS             1068..1439
                     /gene="rpl7ae"
                     /locus_tag="VKL21_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HMB46152.1"
                     /translation="MAQPTYIKFEVPAELSNKALEALEMARDTGKIKKGTNEATKAIE
                     RGIAKLVIIGEDVNPPEIVMHLPALCEEKNTPYIYVKKQVELGAASGLSVGNGAAAII
                     EPGKGKELVEEVAQKVQALKK"
     gene            1444..1659
                     /locus_tag="VKL21_10055"
     CDS             1444..1659
                     /locus_tag="VKL21_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HMB46153.1"
                     /translation="MADEDAGYPAEIIEIVGKTGMHGEAMQIQCRVLDGRDKGRIITR
                     NVVGPVKLGDILILLETSREAKKLMSR"
     gene            1664..1858
                     /locus_tag="VKL21_10060"
     CDS             1664..1858
                     /locus_tag="VKL21_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HMB46154.1"
                     /translation="MEKKKCSFCGGAIEPGTGKLYARKDGTVFYFCSSKCQGNLNLGR
                     IPRKVAWTEAGRKARGKTAQ"
     gene            1868..>2161
                     /locus_tag="VKL21_10065"
     CDS             1868..>2161
                     /locus_tag="VKL21_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMB46155.1"
                     /translation="MERTYVMVKPDGVQRGLIGEVLNRIERRGLKIVAMRMNTMSLDS
                     AKKHYAEHAQKPFFSSLISFITSGPSVSMVVEGKNAMSVMRAVNGATNPVNAAT"
BASE COUNT          731 a          394 c          504 g          532 t
ORIGIN      
        1 tgtgtacatc aatgtataat tgagtacatt gtatataaaa gttatccttt aatataagaa
       61 aagtaattag gggaagcatg gatcagcagt tggcatataa attactcgcg ggctttcttg
      121 tatttacaat gttaggctcg gtattcgctt attttttcat tggaggaaga gacgacacca
      181 cgcaggaccc ccagactcca gatactaacc tggataaata caaccctgag ttctggacgg
      241 ttaaccagcc attcaattct atttccgatg ccttgagcat gacacctgtg gatgcggaag
      301 aggcatattt tgtggatctt gaaaacatgc ctattaaaat gttagagtgg acaagatccg
      361 gaaatagaat taccagtgga ttgatccagg aaatggatac aaagatctat aaatccaata
      421 ctactaaatt gtattatgca ttgcttaaag gtggaaataa tgaaacgtca ccaccattcc
      481 ttctgttaag tactatgtcc cctccgaaaa atgattttga ttattacgtg attccaaata
      541 cgagtaatat ccttcaaagg caagatacag gagctataaa tataatggga actcctgtaa
      601 tttatgcacc cacggataaa attgccggcg acgttattga tatcatttac agcttgaata
      661 aatcggttac tgcttatgac caatacgaaa aattgcttaa taaagtggaa cctgtggctt
      721 accagatggt aagttcaaat gtgagttttg caaggcaatt ttatgttggg atcggattga
      781 acaatggtag ttatgaaagg aacacagtct atcttaatgc aaacacatct gttttgatga
      841 aactaaacca gtcccgggct aacagcacag aaagaggttt tcaacaatat atcgttaacc
      901 agagtggaaa ttacaccact gttaaaatag tatcaccgga actgttaaca gttctgagtg
      961 aggagacatc ctaggcgcaa aatatatagc atatcatgct gtagaaagcg ccgatacttt
     1021 taatcaaatg aatgatgaag gataaaatcc tgaaggaagt aattaaaatg gcacaaccaa
     1081 cctatataaa atttgaagtt cccgcagaac tatccaataa agcattagaa gctcttgaga
     1141 tggcacggga taccggtaag ataaagaaag gaacaaacga agccacaaaa gcgatcgaac
     1201 gtggaatagc aaaacttgta ataataggcg aagacgtgaa tccacctgaa attgtgatgc
     1261 atcttcctgc actttgcgaa gaaaaaaata caccatatat atatgtgaaa aaacaggtgg
     1321 aacttggagc agcaagcgga ttaagtgtgg gcaacggcgc agcggcaata attgaacccg
     1381 gaaagggtaa agaactggta gaagaagtgg cacagaaagt ccaggcattg aagaagtgaa
     1441 atcatggctg atgaagacgc aggatatcct gccgaaataa ttgaaattgt tggcaagacc
     1501 ggtatgcatg gcgaagccat gcagatacag tgccgggtac tggatggcag ggataagggc
     1561 aggatcatta cccgaaatgt cgtagggccg gtcaaactcg gggatatcct gatactgctt
     1621 gagacctcac gagaagcaaa gaaattaatg tcaaggtgag attatggaaa aaaagaaatg
     1681 ctcattctgc ggaggtgcaa tagaacccgg taccggcaag ctttatgccc ggaaagatgg
     1741 gactgtgttt tatttctgtt cctctaaatg tcagggaaat ttaaacctcg gtagaatccc
     1801 ccgaaaggtc gcatggaccg aagcgggaag aaaagccagg ggaaaaacag cacagtaagg
     1861 tgagacattg gagcgtactt acgtgatggt caaacccgat ggagtgcagc gtggacttat
     1921 cggtgaagtt ttaaatcgga tcgagagacg cggactcaaa atagtggcta tgcgaatgaa
     1981 taccatgtca cttgattccg caaagaaaca ctatgctgag catgcgcaga aaccattttt
     2041 cagctccctg ataagtttca taacatcagg tccatctgtt tcgatggtag tggaaggtaa
     2101 gaatgccatg tctgtgatgc gtgctgtcaa tggagcaacc aacccggtga atgctgccac
     2161 a
//
LOCUS       DATGBN010000403         6809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_149568, whole genome shotgun sequence.
ACCESSION   DATGBN010000403 DATGBN010000000
VERSION     DATGBN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149568"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VKL21_10070"
     CDS             <1..708
                     /locus_tag="VKL21_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HMB46156.1"
                     /translation="YFKRSFFSCSVSIFKTRGMLMKILTEHEAKKLLLKYGIPVTKES
                     IANSPDEAFAIASQIGTPVAMKISSPDISHKSDVGGVVLNVGAHEARSTYDKIISQIK
                     KVAPLARIEGILVQQMAPQGQELIVGLKKDAQFGHALMFGLGGIFVEVYKDVSFRVVP
                     IEKSEALDMISQIRGYPILKGIRGRKPANIEAIAHVLVSVSQMAQKENIIELDINPLM
                     AGETGVVTVDARAMIEE"
     gene            717..1181
                     /locus_tag="VKL21_10075"
     CDS             717..1181
                     /locus_tag="VKL21_10075"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMB46157.1"
                     /translation="MADKIDVIYSKVLDRYIEILYYTKLHSLRFVPPGKQLKEKKTND
                     ATFELENYFNGSQIDFSFDVDLVHLSPFEQKVLTQTSKIKYGKTITYSELAEKIGSRA
                     YRAVGNALGKNPVPIIIPCHRVVAKKGIGGYSEGLEIKTRLLELEKRNINNL"
     gene            1209..1280
                     /locus_tag="VKL21_10080"
     tRNA            1209..1280
                     /locus_tag="VKL21_10080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1242..1244,aa:Arg,seq:gcg)
     gene            complement(1331..2194)
                     /gene="sucD"
                     /locus_tag="VKL21_10085"
     CDS             complement(1331..2194)
                     /gene="sucD"
                     /locus_tag="VKL21_10085"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMB46158.1"
                     /translation="MGILINKDTRVLVQGITGHEGSLQTKQMIDFGTNIVGGVTPGKG
                     GSQVQKQPVYNSVKEALEETDVDASVIFVPPQFAADGIFEAIDNGIELIVCITEGIPV
                     HDMMRVWAYLKTSSSRLLGPNCPGITTPGASKIGIMPNPIHMPGNIGVVSRSGTLTYE
                     IVDTLSKNGIGQSTSVGIGGDPVNGTSFVDVLELFEEDPETHAVVLVGEIGGTAEEDS
                     IEFIKQMSKPVISYIVGVSAPPGKTMGHAGAIISMGVGTALEKIQAFEKAGITVAKSP
                     SEIVRHVKNIT"
     gene            complement(2175..3281)
                     /gene="sucC"
                     /locus_tag="VKL21_10090"
     CDS             complement(2175..3281)
                     /gene="sucC"
                     /locus_tag="VKL21_10090"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMB46159.1"
                     /translation="MKLYEYEAKDVFSKYNIPIPQSSLVSDIYGAGEAAQKMRDVVIK
                     AQVHTGGRGKAGGIARASSPLDAKNGAKRILGMSIKGFPVKKVLIEEYREPIKEMYLG
                     ITIDRKAKCPVIMASPEGGIDIEDIARNSPEKITMVHIHPLAGIHEYQARMLANSLDR
                     NKIPDLIDIIKKLYRVFIENDCQLVEINPLAQTKDGIIALDAKMVIDDNALFRQDFPE
                     EENGSLEEIARKNGMSYVGLDGDIGCIVNGAGLAMATLDMVKHYGGAPANFMDVRAGA
                     NEEQVKKALEIVSSGKNVKSIIINIFGGLTKCDEAARGIIDILPDLKIPLVIRLSGTN
                     EKEGRAMLKKYGIHLAASTEEAAKGVVEIGNSNK"
     gene            3460..4425
                     /locus_tag="VKL21_10095"
     CDS             3460..4425
                     /locus_tag="VKL21_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HMB46160.1"
                     /translation="MLKEEIWIEKYRPKRLEDVVGQVEIIKRLKSYIQSRNLPHLLFS
                     GPPGVGKTASAICVARELFGETWHNNFTELNASDERGIDVVRTKIKNFARTAPLGEAE
                     FKIIFLDEADALTSDAQSALRRTMEKYTSSCRFILSCNYSSKIIEPIQSRCAVYRFKP
                     ISSAAVEERVKHIAKIEGLNLTDDGLEVIRYVGAGDMRRAINALQAAALLDKTVDLDA
                     IYKTTATAKPEEVVELIKLALDGHFMKARAQLDYLLIEQGLSGEDIIGQIYRAMFDMQ
                     IPDKLKVDLIDRIGEIDFRMAEGANERIQLEALIAHIILCGSTKK"
     gene            4422..4934
                     /locus_tag="VKL21_10100"
     CDS             4422..4934
                     /locus_tag="VKL21_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HMB46161.1"
                     /translation="MTLEAFLLDLFSSIPPWLAVIIMAALPIVELRLAIPLAILNPPY
                     GFGMDPVWAFSLSVIGNMLPVIPLLLFLEPVSNYLRRWKTWDRFFTWLFTRTHRNHSE
                     SFEKYGSIGLAIFVGIPLPATGAWTGCAAAFVFGFKFRNALIAIFAGVIIAGVVMTLL
                     TVGINIFNGI"
     gene            4997..5506
                     /locus_tag="VKL21_10105"
     CDS             4997..5506
                     /locus_tag="VKL21_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMB46162.1"
                     /translation="MGKRIRIINEPSDIVPLLRAFGIESHKKVFESLQTGWKTDEDIE
                     KEVGFNPTESLGILKKSGFVESKWRMPQPGKKPEKEYHSSYSRVQVNFECSVEDLSDM
                     IMVTFKSDNELRNYLDLIEKEVKAGNQSMNGLQRVIDKSIMYIRAVARRSPILTVKGQ
                     RIEIFGDTE"
     gene            5503..6285
                     /locus_tag="VKL21_10110"
     CDS             5503..6285
                     /locus_tag="VKL21_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMB46163.1"
                     /translation="MILRSKKEITKFQILVEIAGHQPDVMQKEIANRIGITPQAVSEY
                     IKDLVKEGFLYSDGRVRYRVTKNGVEWVLERAIELKKYAHFVMEDIVSHVSVATAIAR
                     EKFSRGDAVSLMMENGLLYAGKDGFVTGITISDADEGEDVGVTDLKGMISFPPVTITI
                     CKVPRVEKGGSRCVDYEILKERSQNKPYIAAIGVEALVSLRKISVWPNILFGAKESVV
                     EAAFHGLSSLVVSVDEEVPGLLNRLESEGLNYEVIDLGKSGT"
     gene            complement(6331..>6809)
                     /locus_tag="VKL21_10115"
     CDS             complement(6331..>6809)
                     /locus_tag="VKL21_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HMB46164.1"
                     /translation="TCGTFLEETEKVSKNYPGIELEEFHIDNIAQQLIKNPQIFNNKV
                     LLSTNLFMDILSEECSALVGSIGLIYSANIGDKYAMFEPAHGSAPKYAGLDKVNPVAT
                     VLAGAWLLDYLGEKNNSKAIFEATEKVISEGKFLTYDLGGNAKSSKMVDEIIRKIT"
BASE COUNT         1895 a         1444 c         1499 g         1971 t
ORIGIN      
        1 tattttaaaa ggagcttttt ttcatgttcc gtaagtattt tcaaaaccag gggaatgctt
       61 atgaaaatac ttacggaaca tgaggcaaaa aagctccttt taaaatacgg gatacccgtt
      121 accaaagaga gtattgctaa tagccctgat gaagcttttg caatagcatc acaaatcgga
      181 actcctgtgg ccatgaaaat atcatcgcct gatatttcgc ataaaagcga tgtgggcggc
      241 gtagttctaa atgttggtgc gcatgaggca aggagtacct atgataagat catatcgcaa
      301 attaaaaaag tggcgcctct tgcaaggatc gaagggatcc ttgtccagca gatggcgcct
      361 cagggtcaag agttaattgt tggccttaaa aaagatgcgc agttcggtca tgcgctgatg
      421 ttcggtcttg gcgggatatt cgttgaagtg tataaagatg tttcattcag ggtcgtgcca
      481 atcgaaaaaa gtgaagctct tgatatgata tcgcagataa ggggatatcc tattcttaaa
      541 ggtatcaggg gaaggaaacc tgcgaatatt gaggcgatag ctcatgttct tgtttctgtt
      601 tcccagatgg cgcaaaagga aaacattatc gagcttgata tcaatcccct gatggcaggt
      661 gaaacaggag ttgttacagt ggacgcaaga gcgatgattg aagaataggt cagaaaatgg
      721 cagataaaat tgatgttatt tattcaaaag ttcttgatcg ttatatagaa atattatatt
      781 atacaaaatt gcattcactc aggtttgtcc cgccgggaaa acagcttaaa gaaaagaaga
      841 caaatgatgc gactttcgaa ttagagaact attttaatgg cagtcaaatt gatttctcat
      901 ttgatgtgga cttagttcat ctttctcctt ttgagcaaaa agtgcttacc cagaccagca
      961 agatcaaata cggtaagacg atcacctatt cggaactggc agaaaaaata ggcagcaggg
     1021 catatcgggc agtgggaaat gctcttggaa aaaatcctgt ccctattatt attccctgcc
     1081 acagggtcgt ggcaaaaaaa ggcattggcg gatattcgga aggtttagaa ataaaaacca
     1141 gattgcttga gcttgaaaaa agaaatatca ataaccttta atatgtcaaa aaggaatgtg
     1201 gggtcagagc cccgatgggg tagcggatat cctttgagat tgcggatctt aagacccggg
     1261 ttcaattccc ggccggggcg ctcaaattct ttttaaatgc cccgctccag acaaatattt
     1321 tttcgtccca tcacgttata ttcttcacat ggcgcacgat ttccgaaggg ctttttgcca
     1381 cagtgatccc ggctttttca aatgcctgta ttttttcaag agctgttccg acacccattg
     1441 atatgatagc gcctgcatgg cccatggtct ttccgggcgg cgcagaaaca cccacgatat
     1501 aactgatgac aggtttactc atctgcttta tgaattcaat ggaatcctct tccgctgttc
     1561 ccccgatttc tccaaccaat actacagcat gtgtttcggg atcctcttca aacaactcca
     1621 gtacatccac aaacgatgtt ccgttcaccg ggtctccgcc gataccaaca gaagttgact
     1681 gcccgatacc attttttgaa agcgtatcaa cgatttcata tgtcagggtc ccgcttcgtg
     1741 atacaactcc tatattgcct ggcatgtgta tgggatttgg cattatcccg atctttgatg
     1801 cgcctggcgt tgtaatgccc gggcaattgg gtcccaaaag ccttgaggat gacgttttaa
     1861 ggtatgccca cactcgcatc atatcatgca cagggatccc ttcagtaatg catactatca
     1921 attcgatccc attatcgatg gcttcaaata tcccatcggc tgcaaattgc ggcgggacaa
     1981 aaataacaga tgcgtccaca tctgtctctt ccaaagcttc tttgacagaa ttataaacag
     2041 gctgtttttg aacctgagag cccccttttc caggggtaac gcctcccact atattagttc
     2101 caaaatcaat catttgtttt gtctgaagcg atccctcatg tcctgtgata ccctgcacaa
     2161 gcacccgggt atctttattt attagaattc ccaatctcta caactccttt agctgcctct
     2221 tcggtggatg ctgccaggtg tatcccgtat tttttaagca ttgctctacc ttctttctca
     2281 tttgttcctg aaagcctgat aacaagtgga attttcaaat ccggcaatat atctataatt
     2341 ccccgcgcag cctcatcgca ttttgtgaga cctccgaaaa tattaatgat tattgattta
     2401 acattcttgc cggatgagac tatttccagc gcttttttta cctgctcttc attagctcct
     2461 gcgcgaacgt ccatgaaatt ggctggcgcc cctccataat gtttgaccat atcaagtgtt
     2521 gccattgcaa gacccgcccc atttactata catccgatat cgccatcaag cccaacataa
     2581 ctcataccgt tctttctggc gatttcctca agagaaccgt tttcctcttc cgggaaatcc
     2641 tgcctgaata aggcgttatc gtcaattacc atttttgcat caagcgcaat gatcccgtct
     2701 ttagtctgtg ccagtggatt aatctcaacg agctggcaat cattctcgat aaaaacacga
     2761 tacagtttct taataatatc aataaggtcc gggatcttgt tcctgtccaa tgaatttgca
     2821 agcatccttg cctggtattc atgtatccct gcaagcggat ggatatggac cattgtgatc
     2881 ttttccggag aattccttgc gatgtcttcg atatcaatcc ctccttccgg acttgccatt
     2941 atgacaggac attttgcttt cctgtcgatg gtgataccca gatacatctc ttttatgggt
     3001 tccctgtatt cttctatcag cacttttttc acgggaaatc cttttatcga catcccaagt
     3061 atacgcttag caccgttctt tgcatccaat ggacttgacg ctctggcgat accaccggct
     3121 tttcccctcc ctccagtgtg tacctgggct tttatgacca catccctcat tttctgtgcg
     3181 gcttcccctg cgccatatat atctgaaaca aggctactct gggggatcgg gatattatat
     3241 tttgaaaata catctttggc ttcatattca tagagtttca taagcatctc cctctggaat
     3301 cacataatgt attatctata gccttcttat ttaaacatgt ctggaatcaa tttgtgagat
     3361 tttttattat cttccagaat aatttaaaaa gtctccaatt tgcgagttct tgctcttcct
     3421 ataagttatt aataatacct gcttcttaat aaggtgggaa tgctaaaaga ggaaatctgg
     3481 atcgaaaaat accgtcccaa gagacttgag gatgtagtgg ggcaggtcga aattataaag
     3541 cggcttaaat cctatatcca gtcaaggaac cttccgcacc ttcttttctc cggacccccg
     3601 ggggtgggaa aaacagcctc cgcaatttgc gtagccaggg agctttttgg cgagacctgg
     3661 cataacaatt tcactgagct gaatgccagt gatgaacgcg gtattgatgt agtgaggaca
     3721 aagataaaga actttgcaag gactgcgccc cttggcgaag cggaattcaa gataatcttt
     3781 cttgacgagg ctgatgctct tacttcagat gcccaatcgg cgctgcggag gacaatggaa
     3841 aaatacacca gttcctgccg ctttattcta tcatgcaact attcttcaaa aataatcgaa
     3901 cccatacaat cacgatgcgc tgtttacagg ttcaaaccca tatcaagcgc agcggttgag
     3961 gaaagggtga agcatatcgc caaaatagaa ggactgaatt tgacagatga cggactggaa
     4021 gttatacgat atgtgggggc aggggacatg cgccgtgcca ttaatgcctt gcaggcggct
     4081 gcactgcttg ataagaccgt tgatctcgat gctatatata agaccactgc tactgccaaa
     4141 cccgaagaag tagtggaatt gattaaactt gcgcttgatg ggcatttcat gaaagcccgc
     4201 gcacagcttg attatttatt gatcgagcag gggctttcgg gtgaggatat aattgggcag
     4261 atctatcgag ccatgtttga tatgcaaatc cccgataaac tgaaagtcga tttgatcgat
     4321 cgtataggcg agatcgattt caggatggct gagggcgcta acgaacgcat ccagcttgaa
     4381 gcattgatcg cacacattat cctttgcggc tcaactaaaa aatgacactc gaagcatttc
     4441 ttctggattt attcagttca atacccccat ggcttgccgt tataattatg gcggcacttc
     4501 cgattgtgga attgcggctt gccataccat tagcgatctt gaatccccca tatggattcg
     4561 ggatggaccc ggtttgggct ttttccctgt ctgtaattgg taatatgctg ccggtcattc
     4621 ctcttctcct ttttcttgag ccggtgtcaa actatctgcg caggtggaaa acctgggaca
     4681 ggttttttac ctggcttttc acaaggacac atcgtaatca cagcgaaagc tttgaaaaat
     4741 acgggtcgat cgggctggcg atatttgtag gcataccttt acctgcgacg ggggcatgga
     4801 caggctgtgc ggcggcattt gtttttggat tcaagttcag gaatgctctt attgcaatat
     4861 ttgcaggtgt tataatagcc ggggtggtaa tgactctcct gacagtcgga ataaatatat
     4921 ttaatggtat atagtttgtt caaaacgttt atccttttat aagactaata taggatgcta
     4981 ttgagagaga tcatccatgg gaaaaagaat tagaattatc aatgaacctt cagatattgt
     5041 tccgctttta cgtgcgtttg gtatagaatc ccacaagaag gtatttgaaa gccttcaaac
     5101 tggctggaaa accgatgaag atatcgaaaa agaagttggt ttcaacccga ctgaaagtct
     5161 gggtattttg aaaaaaagcg ggtttgttga aagtaaatgg agaatgccgc agcctggaaa
     5221 aaagcctgaa aaggaatacc attcatctta ttcaagagtg caggtcaatt ttgagtgctc
     5281 tgttgaggat ttaagtgaca tgataatggt cacattcaag agtgataatg aattaaggaa
     5341 ctatcttgat ctgatagaaa aagaagtaaa agcagggaac cagtctatga acggcctcca
     5401 gagggttata gataagagca taatgtatat ccgcgcagta gcaagacgtt cgccaatcct
     5461 tacagtgaaa ggacagagga ttgaaatctt cggggatacc gaatgatact tcggagtaaa
     5521 aaagaaataa ccaaattcca gatcctggtt gaaatcgcgg ggcaccaacc cgatgtaatg
     5581 caaaaggaaa tcgcaaacag gataggcata actccacagg cggtttcaga atacatcaaa
     5641 gacctggtca aggaaggttt tttatattcg gatgggcggg ttcgttaccg ggtcacaaag
     5701 aatggtgtag aatgggttct tgaaagggcg atagagttaa aaaaatacgc tcattttgtt
     5761 atggaagata tcgtgagcca tgtctctgtg gctactgcta tcgccaggga aaaattttcc
     5821 aggggagatg cggtatcatt gatgatggaa aatggtcttt tatatgcggg gaaagatggt
     5881 tttgtcaccg gaattacaat ttcggatgcg gatgaaggag aagatgtggg agttacggat
     5941 ctaaaaggga tgataagttt tccaccggtc actataacta tctgcaaagt gcctcgtgtt
     6001 gaaaaaggag gctcaagatg cgtcgattat gaaatactaa aggaacgatc gcaaaacaag
     6061 ccttatattg cagcaatagg ggttgaggct cttgtatccc tgcgtaaaat aagcgtctgg
     6121 cccaacattc ttttcggagc taaagaatcg gtggttgaag ctgcatttca tggcctgtcc
     6181 tcgcttgttg tttcagtgga tgaggaagtt ccaggcctgc ttaacaggct tgagtccgaa
     6241 ggtttgaact atgaagtaat agatctggga aaatccggaa cctgagattt ttcccattct
     6301 tcacacattc tttcataaca atcagatttt tcatgtgatt ttccggatta tttcatccac
     6361 cattttgctt gattttgcat tccctccaag atcataagtc aggaatttgc cttctgaaat
     6421 aaccttttct gttgcctcaa agatcgcttt tgagttattt ttctcaccaa gataatccag
     6481 aagccatgca cctgcaagaa ctgttgccac aggatttacc ttatcaagcc ccgcatattt
     6541 tggcgcagag ccgtgcgccg gctcaaacat cgcatattta tcaccgatat ttgctgaata
     6601 aatgagcccg atgcttccta caagcgctga gcactcctcg ctcaggatat ccatgaagag
     6661 atttgttgaa agaaggactt tattgttgaa tatttggggg ttcttgatga gctgctgtgc
     6721 aatgttatct atatgaaatt cttcaagctc gatacccgga taattctttg ataccttttc
     6781 agtttcctca aggaaagtgc cgcaagtct
//
LOCUS       DATGBN010000404         4840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_227275, whole genome shotgun sequence.
ACCESSION   DATGBN010000404 DATGBN010000000
VERSION     DATGBN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227275"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VKL21_10120"
     CDS             complement(<1..515)
                     /locus_tag="VKL21_10120"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB46165.1"
                     /translation="MKISSIDFLLQGIIKAFQLIFSLNPYILSITGVSIEVSGTATIL
                     ATLTAIPLAFAISFMHFRGKQGLITLVNTGMGLPPVFVGLFVFLMIVPVGPLGFLNII
                     YTREAMIFAQYILITPIITGISLAAIKAVPRSIIETVFTLGGTQQDAVIETLKEARFG
                     IITAVLAGLGRA"
     gene            complement(550..1458)
                     /locus_tag="VKL21_10125"
     CDS             complement(550..1458)
                     /locus_tag="VKL21_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMB46166.1"
                     /translation="MNNLFTKIILVSILISLFSGCVQQAPTGNVTIPSSINTITPTST
                     TSATQKIILATTTSTCDTGLLDVFNKKFEEENNVIISTICEGTGKAIATGELGAADVL
                     MVHSVQAELNAVANGNFINRTYMMYNYFVIIGPESDPAGIGNASNATDAFIRIAAKQS
                     PFISRGDNSGTHMMELSIWKAANITIAGTWYQSVGKGMGDTITTADIKNGYTLSDRGT
                     YLAMKDKIKLGILFESDQKYLFNPYHVMAVNPAKFPNVKYDIAMKYIQYATSAQGQDI
                     IRNYGMDKFGEALFVPEEDAGNVTSP"
     gene            complement(1549..2097)
                     /locus_tag="VKL21_10130"
     CDS             complement(1549..2097)
                     /locus_tag="VKL21_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HMB46167.1"
                     /translation="MKRTQARAKLWLINNKEEPIIGEGKAALLEAINQEGSLNRACKK
                     MQISYKHAWLLLKEIEESAGEPIIITQRGGPGQGTSLTEKALNLLEEYNTYQNVLNQT
                     VYDKTFWEAIGLKLSARNQMKGKIVEIEKEGLISKIKISIEPAIITAIITKEAADAMD
                     IKKDDSVVAVVNATEVMIGKEE"
     gene            complement(2110..2475)
                     /locus_tag="VKL21_10135"
     CDS             complement(2110..2475)
                     /locus_tag="VKL21_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMB46168.1"
                     /translation="MTTKAELYLNALSELENSTGDVISTAIITHDGLIMASTKSDTMH
                     RETFAAYGAVAFKRADETMEELTGENIDMLLYDSKTNRVVTVRAGEHALLIVFTGKNV
                     QMGMVLMNAQNTALKIKEL"
     gene            complement(2484..3194)
                     /locus_tag="VKL21_10140"
     CDS             complement(2484..3194)
                     /locus_tag="VKL21_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46169.1"
                     /translation="MVNSSYFYGFEETLGPHVILSNLFWWTSLILVMTAYKNRRIWNV
                     GEMPWAYLFLTFFFFGIRELGHLDKSSMVGSIRYVFGILSAIFMTSALIFIYLKIYKR
                     EKISKNMSFIPLIFALIFPILFIYLYFSGTQTQNLWNIFFNIENIIWIIGGSITIYTT
                     YMLGTKSTGGFVYVFMFFQFAAIFAILWKFLGIIGNISCPIPYSIREFLETMFGVFAI
                     ISMYVLEKMLRNLSRRIS"
     gene            3334..3552
                     /locus_tag="VKL21_10145"
     CDS             3334..3552
                     /locus_tag="VKL21_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HMB46170.1"
                     /translation="MEYLDLQGKVCPFVLFHTKKKLETIPAGEKLEVMADDPTAKETI
                     SGWCKAHRHEIVEIEKQGNKIKITILKH"
     gene            3715..4293
                     /locus_tag="VKL21_10150"
     CDS             3715..4293
                     /locus_tag="VKL21_10150"
                     /inference="COORDINATES: protein motif:HMM:NF044805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46171.1"
                     /translation="METTPTNFNVKDMTENSKNVNATLKVIEIGEVKDIKSQYGDKRV
                     CEVKVADATGSILLSLWDDQIGKIAAGDSISIQNGYISVVRNSMRLNIGKYGKMLLSE
                     EPVVETNTELNMSDKHVEMPDRPRRTGGYGGGSGGGRPSSGGYGSMYGSGGGSGGGFG
                     GGRGGSGGGRSGGGGRDRGRDSRDKKGGGKRR"
     gene            4356..>4840
                     /locus_tag="VKL21_10155"
     CDS             4356..>4840
                     /locus_tag="VKL21_10155"
                     /inference="COORDINATES: protein motif:HMM:NF020764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HMB46172.1"
                     /translation="MLENIPETFLSITDKEFRILVAIENKMKFYEWVPIEEIVNFTRF
                     DLKEVEYLLSNLAHNKLIHRNDKTYEGYRIYFEAYDLLALNAFVKRGSVNALGEVIGI
                     GKESIVFDATGGIIDRHVIIKLHREGKSSFRHVRVKRETTGERRHLSWLYAARLAAKK
                     E"
BASE COUNT         1433 a          974 c          995 g         1438 t
ORIGIN      
        1 gctctcccaa gtccggcaag taccgctgtg atgataccaa atctcgcctc ttttaaagtc
       61 tcaatgacgg catcctgttg cgttccacca agcgtaaaaa cggtctcgat aatagatcgg
      121 gggactgcct ttattgcagc aagagaaatg ccagtgatta ttggtgttat tagaatgtac
      181 tgggcaaata tcatcgcttc tcgtgtataa ataatattca ggaaccctaa aggaccaaca
      241 ggcacgatca tcaaaaaaac aaagagacca acaaaaaccg ggggcagtcc cattcctgta
      301 tttacaagtg tgataagtcc ctgtttccct cgaaaatgca tgaaggatat ggcaaatgcc
      361 agtggaatcg cggtcagggt cgcaaggatt gtggcggtgc cagatacttc tattgaaact
      421 cccgttatgc tgagaatata aggatttaaa ctgaatatca gttgaaatgc tttgatgatt
      481 ccttgaagaa gaaagtcaat actgctaatt ttcaaactct cctttattat tggaattgtc
      541 tttaatattt cacggacttg tcacgttccc cgcgtcttcc tccggcacaa aaagcgcttc
      601 tccaaattta tccatgccat aattccttat gatatcctgt ccctgggcag atgttgcata
      661 ctgaatatat ttcattgcaa tatcgtattt cacattcggg aatttggcag ggttcacagc
      721 catcacatgg taaggattaa aaagatattt ctggtcagat tcaaataata tccccagttt
      781 tattttatcc ttcattgcca ggtatgttcc cctgtctgag agtgtatagc cattcttgat
      841 gtcggcggtt gtgatggtat ctcccatgcc tttgcccact gattgatacc atgttccggc
      901 gattgtgatg ttcgcagcct tccatatcga taattccatc atatgtgtgc ccgaattgtc
      961 gcctctggaa atgaatgggg actgctttgc agcgatcctg atgaatgcat cggtagcatt
     1021 gcttgcattc ccgatacctg ccggatcgct ttcgggcccg atgatcacga aataattgta
     1081 catcatatag gtgcgattta tgaagttgcc gttagctaca gcatttaatt ctgcctggac
     1141 cgaatgcacc atcaatacgt ccgctgcccc cagctcacct gtggctattg ctttacctgt
     1201 accttcacaa atcgttgata ttattacatt attctcttcc tcgaactttt tattaaagac
     1261 atccaacagc cctgtatcgc aggtagaagt ggtcgttgcc aaaataattt tctgcgtggc
     1321 agatgttgtt gatgttggag ttattgtatt gatggatgaa ggaattgtta cattcccggt
     1381 gggtgcctgc tggacacagc cggaaaacag cgatattaaa atcgaaacta atattatctt
     1441 tgtaaagaga ttgttcatat ttacacctta tgttcactca aacataacgc atgttaataa
     1501 aagtttcgaa aaaaatcact tttccatttc cgcacctaca tcttatggtt attcttcttt
     1561 tccgatcatt acttccgtag cgttcacaac cgccacgact gaatcatcct tcttgatatc
     1621 catggcatct gcggcttctt ttgtaatgat tgccgtaatt attgcgggtt caatggaaat
     1681 cttgatcttt gagataaggc cttctttctc aatctcgact atcttcccct tcatctgatt
     1741 cctcgcagaa agtttcaatc cgatcgcctc ccagaaagtt ttgtcatata ctgtttggtt
     1801 aagtacattt tgataagtat tatattcttc aagcaggttc aatgcttttt ccgtcaggga
     1861 tgttccctgc cccgggcccc cgcgctgtgt gattattatg ggctctccgg cgctttcttc
     1921 aatttctttt aacagcagcc atgcgtgctt ataagaaatt tgcattttct tgcatgccct
     1981 gttcagggaa ccctcctggt ttatggcttc aagaagagcg gctttacctt ctcctattat
     2041 tggctcttct ttgttattta tcagccataa ttttgctctt gcctgtgtcc ttttcatctt
     2101 cttaagaaat cacaattcct ttattttaag ggcagtattt tgcgcattca taagtaccat
     2161 acccatctgt acattctttc cagtaaaaac tatcaagagg gcatgttctc ctgctcgaac
     2221 agttacaacc ctattagttt ttgaatcgta taacaacata tcaatgttct cacctgttaa
     2281 ctcttccatt gtttcatccg cccttttaaa tgcaactgcc ccgtaggctg caaatgtttc
     2341 cctgtgcatt gtatccgatt ttgtgcttgc cattatcaga ccgtcatgag ttatgatagc
     2401 tgtgctgatg acgtctcctg tggaattctc caattccgaa agtgcattta aatataattc
     2461 ggctttagtg gtcatatttg tccttatgaa atgcgtctgg atagatttct caacattttt
     2521 tcaaggacat acatggatat aattgcgaac accccaaaca tagtctccag gaattcacgg
     2581 atagaataag ggatagggca gctgatattt cctatgatac caaggaattt ccataaaatg
     2641 gcaaaaatag ctgcgaattg gaaaaacatg aaaacataga caaaacctcc tgttgatttc
     2701 gttccaagca tatatgtcgt gtaaatagtt attgatcctc ctattatcca gataatattt
     2761 tctatgttga aaaaaatatt ccatagattt tgggtttgag tccccgaaaa atacaggtat
     2821 ataaaaagga tcggaaatat aagagcaaaa attaatggaa taaaactcat attttttgat
     2881 atcttctctc gtttatatat ttttaaatat ataaatatca aagcggatgt catgaatatc
     2941 gcggataata taccgaaaac gtatcttatt gatcctacca tagacgattt gtccagatgc
     3001 cccaattcac ggatcccaaa aaaaaagaag gtcaggaaca ggtatgccca tggcatttca
     3061 cccacattcc agatacgccg gttcttgtaa gctgtcatca caaggatcaa tgatgtccac
     3121 cagaagagat ttgagagaat cacatgagga cccaaggttt cctcaaatcc atagaaatat
     3181 gaactgttga ccataataat aacgattact atctaaccaa atatattagt ctttaggtgg
     3241 gtgaactaac cctccctttc ggagggatct tttctacccc ttgacatcac gaggaaaaaa
     3301 gatttaatat aacattcatt catagtagtc aatatggaat accttgatct ccagggtaaa
     3361 gtgtgcccct ttgtattatt tcataccaag aaaaaactgg aaaccattcc tgccggtgaa
     3421 aaactggaag taatggcaga tgatcctacg gcaaaagaga caatatcagg ctggtgcaag
     3481 gcacatcgtc atgagatagt ggaaatagaa aaacagggaa ataagatcaa aattaccatt
     3541 cttaaacatt gataggattt tctccgtgtc aatttattga tacagttatg ggtcaatgag
     3601 aaactatatc atctactttt acaatttaat atttacttct tctaactcaa atacaggtga
     3661 atgccatctg ggaaaaagtg atgcaggaag ttaaaataaa agcaaggtat tacaatggaa
     3721 acgacgccta caaattttaa tgtaaaggac atgaccgaga attcaaaaaa tgttaacgcc
     3781 actctaaaag ttattgaaat aggagaggta aaggacataa aatcacaata tggtgataag
     3841 cgggtctgtg aagtaaaagt tgcagatgca acaggaagca tcttattatc cttatgggat
     3901 gaccagattg gcaaaattgc agcaggagat agtatctcaa tccagaatgg ttatatttcc
     3961 gtagtcagga attcaatgag attgaacata ggaaaatacg ggaagatgtt gttgtcagaa
     4021 gaaccggtcg ttgagaccaa tactgaactt aatatgtctg ataaacatgt ggagatgccc
     4081 gaccgcccaa gacggaccgg cggatatggt ggaggaagtg gaggaggccg gcccagcagc
     4141 ggaggttatg gcagtatgta cggcagcggc ggcggttcag gcggcggatt cggcgggggc
     4201 cggggcggct caggcggagg tcgcagcggt ggcggcggta gggaccgcgg cagggattca
     4261 agagataaaa aaggaggagg aaagagaagg taatattccg tcttcttttt ttatcaacct
     4321 tttttatagt gagcagtatt atatactaaa taataatgct tgaaaatatt ccggaaacat
     4381 ttctttctat cacggataag gaatttcgta ttcttgttgc catcgagaat aaaatgaaat
     4441 tctatgaatg ggtcccgata gaagaaatcg ttaattttac aagatttgac ctgaaggaag
     4501 ttgagtatct cctatcaaac ctggcgcata acaagctcat acacaggaat gataagacct
     4561 atgaaggata caggatctac tttgaagcat acgatctcct tgcacttaat gcgtttgtaa
     4621 agcgagggtc ggtaaatgcc ctgggcgaag ttatcgggat cgggaaagaa tcaatcgtat
     4681 ttgatgctac gggcggtatc attgacaggc atgttataat taaactacac agggaaggaa
     4741 aaagcagttt caggcatgtc agggtaaaaa gagaaactac aggggaaagg cggcatcttt
     4801 catggttgta tgctgcgcgc cttgccgcca aaaaggaata
//
LOCUS       DATGBN010000405         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_71754, whole genome shotgun sequence.
ACCESSION   DATGBN010000405 DATGBN010000000
VERSION     DATGBN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71754"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VKL21_10160"
     CDS             <1..1571
                     /locus_tag="VKL21_10160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HMB46173.1"
                     /translation="NELALIDCRRYLASFNRENLVYYVRPKQDAFKQIVEILRSKPGE
                     SGIIYCYSRKNVETITEGLKSSGFRALPYHAGLSSSIRSKNQEKFIKDDVEVLVATIA
                     FGMGIDKPNIRFVIHHDLPKNLEAYYQETGRAGRDGLKSECVLFYSFGDRQKIEYFIN
                     QMSNEKERQIAKNKLNTIIDFSETNLCRRKLLLGYFGEDFKENNCSACDNCLLPKEEM
                     EATGQVMTILSCVDEMDERFGINYVIDVLMGGKTGRILQNGHTSLKSYGSGKNMPKKM
                     WHAFIRELIRRVYLNVEGEYPVLKLGQKSRDILSRSGNCVETEKIYIVKPRTIEKPVK
                     IEPDRVDDGLFEKLRTLRKTLADAQDQPPYLIFNDASLKLMASRRPCNPEDFRKITGV
                     GDKKLERYGGIFISEIRRFCLKQDSTIQPVKKQSSEHVTLEMLDQGMDLDEITLKRNL
                     SLNTIYAHIEKLIISGENVDIGKLIKKEKMESISSAILELGAQNLKPLKEKLGDNFSY
                     GEIRLVRARMMKSS"
     gene            complement(1712..2131)
                     /locus_tag="VKL21_10165"
     CDS             complement(1712..2131)
                     /locus_tag="VKL21_10165"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB46174.1"
                     /translation="MMHREYKLDDNAKENIKSAITEILKTKTEVGFAFLHGSFLKERS
                     FHDIDIAVFICEDGIPENLFEYELSLSSILEGRVRLPMDVKILNHAPIAFRYEVTRGE
                     VIFSRDEEARFRFVENTWNEYLDYKPVAEYIIRELAA"
     gene            complement(2128..2562)
                     /locus_tag="VKL21_10170"
     CDS             complement(2128..2562)
                     /locus_tag="VKL21_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMB46175.1"
                     /translation="MENSAINPNLIGTKISEIQQSLERLGDISSKGKEAFLNEPNLRD
                     SAKYRLITAIEAAISICNHIVARRFKRAPESYSDCFLILHELGVISGELAGRLGDMAR
                     FRNMLVHIYWEINDEKIYTILSDELFDLENYIKEIANYMDKK"
BASE COUNT         1053 a          632 c          730 g          956 t
ORIGIN      
        1 tcaacgagct ggcacttatt gattgccgca ggtatcttgc cagctttaac agggagaacc
       61 ttgtctatta tgtcagacca aagcaggatg cattcaagca gatagtggag atcctcaggt
      121 caaagccggg ggaatccggc ataatttact gttacagcag gaaaaacgtt gaaactatta
      181 cagaaggcct gaaatccagc gggttccgtg ctcttcctta ccatgcaggc ctgtcttcga
      241 gtataagatc aaagaaccag gaaaagttca taaaagacga tgtagaagtg cttgtggcca
      301 cgatcgcatt cgggatgggc attgataaac ccaacattcg ttttgtgata catcatgacc
      361 tccccaagaa ccttgaagcc tactaccagg agactggacg cgcaggcagg gatggattga
      421 agagcgaatg tgtgcttttc tatagcttcg gggaccggca gaagatcgaa tatttcataa
      481 accagatgtc caatgaaaaa gaaagacaaa tagctaaaaa taaactgaat acgataatag
      541 atttctccga gaccaatttg tgccgtagaa agctcttatt gggttatttc ggggaggatt
      601 tcaaagagaa taactgcagt gcttgcgata attgcctgct gccaaaggag gagatggaag
      661 caacagggca ggtcatgacc atcctttcat gtgtggatga aatggatgag agattcggga
      721 tcaattatgt tatagatgtt ctcatgggag gaaagacagg tcgaatcctt caaaatggac
      781 acacatcatt aaaatcatac ggatccggaa aaaacatgcc aaagaaaatg tggcacgctt
      841 ttataagaga actcatacgc agagtctatc tcaatgttga aggtgaatat ccggtcctga
      901 aattgggcca gaaaagcagg gatattttat cccgatccgg aaactgtgtg gaaacagaga
      961 aaatatacat tgtaaaaccc cggactattg agaagccggt taagatcgag cccgataggg
     1021 ttgacgatgg tttgttcgag aagcttcgta cattgaggaa aacactggca gacgcccagg
     1081 accagcctcc ttatttgatc tttaatgacg caagcctgaa actcatggca tcgcggcggc
     1141 cctgcaatcc ggaggatttc cgcaagatca ctggcgttgg ggataagaaa cttgagagat
     1201 atggaggcat ttttataagt gagattcgca ggttttgcct gaaacaggac tcgactatcc
     1261 agcctgtaaa aaagcagtca agtgaacatg tgacccttga aatgctggat cagggaatgg
     1321 accttgatga aatcactttg aaaaggaatt tatccctgaa tactatatat gcccatatcg
     1381 agaaattgat aatttcaggt gaaaatgtgg atatcgggaa gttaataaag aaggaaaaga
     1441 tggaatctat ctcaagcgcc atactggaat tgggtgccca gaatttgaag cctttaaaag
     1501 aaaagttagg ggataatttt tcttatgggg aaataaggct ggtgagggcc aggatgatga
     1561 aaagctctta acaatttttc ttttcttttt cttctaaata ttgcgctggg gtacgacccc
     1621 agaccccccg cggacgcgcc gcccacaggg cggaataaaa tgcaaaatat ttggttgcat
     1681 agaatcgtat tttaaagact acaatatatc ctcacgctgc aagttcccgt atgatatatt
     1741 ccgcaacagg cttgtaatcc aggtattcat tccatgtgtt ctcaacgaat ctaaatcttg
     1801 cctcctcgtc cctgctaaat atgacttccc ctcgtgtgac ttcatatcga aaggctattg
     1861 gtgcatgatt caggattttg acatccatgg gcagtctgac cctgccttcc agaatcgatg
     1921 ataaagataa ttcgtattca aagagatttt ctggtattcc atcctcgcat atgaagactg
     1981 cgatatcgat atcatggaaa ctgcgttctt tcaaaaaact tccgtgaagg aaagcgaatc
     2041 caacttccgt ctttgttttt aatatttccg ttatggctga ctttatgttc tctttcgcat
     2101 tatcgtccag tttatattct ctgtgcatca ttttttatcc atataattgg ctatttcttt
     2161 aatataattc tcaaggtcaa agagttcatc actcaaaata gtatagattt tttcatcatt
     2221 gatctcccag tagatatgca ctagcatgtt gcgaaaacgt gccatatctc ctaaccttcc
     2281 ggcaagttcg ccagatataa cacccagttc atgcaaaatc aaaaagcaat ctgaataact
     2341 ctcaggagct cgtttaaacc ttctggcaac tatgtggttg cagatggata ttgcggcttc
     2401 aattgcagtt attaatctgt acttcgcaga atccctcaga ttgggttcgt tcaaaaaagc
     2461 ttctttacct tttgaagaaa tatcacctaa tctctctaaa gactgttgaa tctcgcttat
     2521 tttagttccg atcagatttg gattaatggc actattttcc atgctttttt tgtttctcct
     2581 tttaagtctt tgccaataga tgatgttcaa aagtaataat ctattcattg aggttatcaa
     2641 ttgcagtttg tgtggggcgc ccggacataa agcttcacgg cagccagcgc gcctgtcagt
     2701 gtggatttcg tgccgctaca gacgagcaga aataataaaa tgtctctgca tctctagaaa
     2761 taatatcaat atctggtata gacgatatat tgggtgggtt ctccgattat tcgacaggat
     2821 ttcgaagtaa cgtgggtaac ggtcacgtaa attcgaaacc tttatataca ggtggtaata
     2881 atgaattcta taatacgtgg atatagtagt gcaaaatgac aaaaccgttt taagaacgga
     2941 atgtcagtct aacaaaccaa ttggaagttt ggttaggaaa gttagaggat atgataagat
     3001 gggaaatatg aggataggaa gagttaatct aagaatacac tgtaaatatt attcttaagg
     3061 ttagttaaat atatgttctt tattttaatc cataaattat attcatagga tatatatcta
     3121 tgtagatagg aaaatagctt aaaagtccta tggaagattc ctgatataaa caggatagat
     3181 aggaaaataa gatacaaaaa ggagaaatgt aaatgaaaaa tctaaaaata gtattttcgg
     3241 ctctattgct atttgcaata ttagtcggaa caggaacggc gctagtaccg ccaccaccag
     3301 caaatcaatt cgcatggaaa tatgatgcga gtattgtcaa tgtgtcagcg acaacgtgta
     3361 ttgcttgtca c
//
LOCUS       DATGBN010000406         5500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_111215, whole genome shotgun sequence.
ACCESSION   DATGBN010000406 DATGBN010000000
VERSION     DATGBN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111215"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="VKL21_10175"
     CDS             <1..1580
                     /locus_tag="VKL21_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HMB46176.1"
                     /translation="SARFLPAILGSTMLFFLIPLRKYIGNIGMLICAFFFALSSSFLY
                     YSRFYREDIFMSFFTLLLLVCIIKFIESYFNMNYSNDRNVPRHGGYSFMRFFYIIIGA
                     LALASLAALKENAYIIMALAFFFLILLFIREKWYVGLMGKIKCRDKKILILFAEIIFF
                     IFLFVVFFSLYYTGNFLYIAGMKDAFMKAVLHWYEMHKIERMGGPFFFYLPIMVLYEL
                     PIFIFGILGIIHYGCCENKKIKILTYSLIYWIIVDVLYLISKTYPESSPFLPVSYVSS
                     SIIVFLPLLIYAILTVLRSKNLFVAFLTYWAMTNFIVYSYIQEKVPWLVLNPLLPLTM
                     IAAIYLNDIIPDLKLNSRKGIITLIIIIVTSSFFVYSSVEFNFKRYTDPAEPLIQAAQ
                     PPQKYALFLDKMNEISMQYEGNSTQIQVTDGEMETQFLWYLRHYNNVQWKVDINSTLD
                     APLIIVHDGDEDKSEADIVKQNMHTDYERIDSAKMSWYWFKESDITLDYLLYRKMDRD
                     PSEYRIVLFYKPKFQES"
     gene            1881..2168
                     /locus_tag="VKL21_10180"
     CDS             1881..2168
                     /locus_tag="VKL21_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46177.1"
                     /translation="MNANELFRNKKLGFASIIGLAFMGFVVGWVFLIPTAAAVFLLYG
                     YVVYNRDQKHRINVNYKPNEAMKAIARREMELNKEKERIMYEQLSGEIKLY"
     gene            complement(2179..3015)
                     /gene="xerA"
                     /locus_tag="VKL21_10185"
     CDS             complement(2179..3015)
                     /gene="xerA"
                     /locus_tag="VKL21_10185"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HMB46178.1"
                     /translation="MPIEIPVSLKKLETELKLRGFSRQTSRMYLFYNRKFLEFIKKNP
                     EEITDDDIKEFLAYKMSDDSLSNASISLIKASLKFLYTEMLGKNMSLIKTPKATKRLP
                     VVLSREEIKNLLDNTENIKHKLLIELLYSTGLRLSECINLQYSNLDMNDGIGWVRQGK
                     GAKDRIFIVSELFRKDLLVYMEKTGADTRGYIFSVNGRKMSPRGLQDAIKVSARRAGI
                     EKKVHVHTLRHSFATHLLENGVDIRKIQKLLGHSNLQTTEIYTQVSSDEIKKIKSPLD
                     ML"
     gene            3835..4719
                     /locus_tag="VKL21_10190"
     CDS             3835..4719
                     /locus_tag="VKL21_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMB46179.1"
                     /translation="MHNNVRFILFQTWSGLEIPCIIDPENKVIAYGLNHKVKYCRIVR
                     RKINGKNRFYLQLICEGTPYQNPKNKIGTEEIGLDVGVGTIATVADTKATLKQFCSEL
                     IPDQKKKRKLQRKLDRSLRATNPDNYNKNGTVKKGKKFKKSNRYKTISKNLAETDLIL
                     AAHRKSLHGKGINEIIAMGTRIQTEKVSYKGWQKRYGRSINMRAPSMFMNSLKRKAEN
                     AGGYLFEFPTQTTKLSQTCHICEQTVKKRLSKRWHICCGIRMQRDLYSAFLAKCVGKD
                     TSALEFARAVRSLILYEL"
     gene            5128..>5500
                     /locus_tag="VKL21_10195"
     CDS             5128..>5500
                     /locus_tag="VKL21_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMB46180.1"
                     /translation="MNLRQPNANEATQTYNRSKSVSPMSGICTRCLDGCRGNCEIFKS
                     SFRGREVIYPGPFGNITAGADKNYPVDYSHLNIQGYAVGAWGLPDGEEPNPDTALFPR
                     VNTETEYGWDKKVRMSVPIFTG"
BASE COUNT         1719 a         1008 c         1063 g         1710 t
ORIGIN      
        1 attccgcccg ctttcttcct gcaattctgg gatcaaccat gctatttttc ttaatcccat
       61 taagaaaata tatcgggaat atcggaatgt tgatctgcgc tttctttttt gctttatcat
      121 cctcgttcct gtattattcc cgtttttaca gggaagacat attcatgtcc tttttcaccc
      181 tgttattact tgtttgtata attaaattta ttgagtctta ttttaatatg aattattcaa
      241 atgatagaaa tgtccccagg catggcggtt attccttcat gagatttttt tacattatta
      301 ttggggcact ggctctggca tcccttgctg cccttaagga aaatgcatac atcataatgg
      361 ctctggcatt ttttttcctg attcttttgt tcataaggga aaaatggtac gtgggtttga
      421 tggggaaaat aaaatgtcgt gataaaaaaa ttttgatctt atttgctgaa ataatatttt
      481 tcattttttt atttgttgtc tttttctcat tatattacac aggaaatttc ctatatatcg
      541 caggtatgaa agatgcattt atgaaagcgg tcctgcactg gtacgagatg cacaagatcg
      601 aacgtatggg aggtcctttc tttttctact tgccgatcat ggttctctat gaacttccaa
      661 tattcatttt tggaattttg gggatcatac attatggatg ttgtgaaaat aagaaaataa
      721 aaatcctgac atattccctt atctattgga taatagttga tgtcctttat ctcatatcaa
      781 aaacttatcc ggaatcaagc cctttcctgc ctgtatctta tgtatcctct tcgataatag
      841 tctttttacc attgttgatt tatgctatcc tgaccgtatt gagatcaaaa aatctttttg
      901 ttgctttcct gacatattgg gcaatgacta atttcatagt atattcttat atccaggaga
      961 aagttccatg gcttgtactc aatcccttgc ttcctcttac aatgatcgct gcaatctacc
     1021 tgaacgatat cattccggat ctgaaattaa attcccggaa aggcatcatt acattaataa
     1081 ttattattgt aacgtcatcg tttttcgtat attcaagtgt cgaatttaac tttaagcgtt
     1141 atacggaccc tgctgaacct cttatacagg cggcgcagcc tccgcagaaa tatgcattat
     1201 ttcttgataa aatgaatgaa atatcaatgc aatatgaagg taattccaca cagatccagg
     1261 ttactgatgg agagatggaa acccagttcc tctggtacct gcgccattat aacaatgttc
     1321 aatggaaggt tgatatcaat tccactcttg atgcgccatt gataatcgtt catgacggcg
     1381 atgaagataa atcagaagcc gatatcgtaa aacaaaacat gcacacagat tatgagagga
     1441 ttgacagtgc aaaaatgtca tggtactggt tcaaagaatc ggatattacc cttgattatt
     1501 tgttatacag gaagatggac agggacccct cggaatacag gatcgtactg ttttataaac
     1561 caaaattcca ggaaagttga aggttattaa aattttttga tattttacga ttcagcttct
     1621 tgaaatctaa atttccgaca ggatttacag gatcgacagg attggattac gtcacacgaa
     1681 ataaattaga gcatcccgtt acgcggattg tactaaaatt cccgttacgc aatcattggt
     1741 caagattccg ttacggtatt tttcagcatg aattcattga taaaagtaaa taaaattgta
     1801 tagtttattc gcatccttac gaaacgatta ttgcctcaga ggagaattat aatacatagc
     1861 aaaaaaaagg agacgtcgat atgaatgcaa atgaattgtt caggaataag aagttagggt
     1921 ttgcctcaat tataggcttg gcctttatgg gattcgtagt aggctgggta tttcttatac
     1981 caacagctgc agcagttttc ctgttatatg gctatgttgt ttataatagg gaccaaaagc
     2041 acaggatcaa tgtaaattat aaaccgaatg aagcaatgaa ggcaattgca cgccgggaaa
     2101 tggagctcaa taaagaaaaa gaaagaataa tgtatgagca actaagtggc gagataaaat
     2161 tatattaaga aaaatatatt aaagcatatc aagtggactc ttaatttttt tgatctcatc
     2221 gcttgatacc tgggtgtata tctccgtggt ctgtaaattt gaatgcccca gaagcttctg
     2281 gattttcctg atatcaacac cattctcaag aagatgtgtt gcgaaagaat gtcgcaaggt
     2341 gtgtacatga accttttttt caatgcctgc cctccgggca gatactttta tggcatcctg
     2401 caggccgcgt ggagacattt ttcttccatt taccgagaat atgtaccctc tggtatctgc
     2461 ccctgttttc tccatataga ccaaaaggtc tttcctgaac aattcggaaa caatgaatat
     2521 tctgtctttt gcgccttttc cctggcgcac ccagcctatg ccgtcattca tatccaggtt
     2581 agagtattgc aggttaatgc attccgaaag ccgtaagcct gtggagtaaa gaagttcaat
     2641 taatagcttg tgctttatat tttctgtatt atcaagaagg ttttttattt cttcacggct
     2701 caacactaca ggaagtcttt ttgttgcttt tggcgtcttt atcagggaca tgtttttccc
     2761 gagcatctct gtgtacaaaa attttaatga agcctttatg agagagatcg aggcattcga
     2821 tagtgaatcg tctgacattt tatatgcgag aaattccttt atgtcatcat ccgtaatttc
     2881 ttccggattc tttttgatga attcaaggaa tttgcggtta tagaagagat acatcctgga
     2941 ggtctgcctg gaaaagcctc ttagtttcaa ttcagtctca agcttcttca gagatactgg
     3001 aatttctatt ggcataatac acctactttt ggcatttcgt gtatataaaa actgcgtata
     3061 atgtagatta tacgcagttt tgggtaattt tgacataatt ttatgaaata tcatgtgttt
     3121 tgaaccggaa gaatgttgtt ttttccggga aaaatttcct atcctgatct atgggtataa
     3181 aagtaaggct tgataaagaa gtatcgctat aaattaaaat aagaggtttg aataaaatta
     3241 aatcttgaat tgccgtaacg ggaatttttt ataaaaattg cgtaacgcgg ttggaacaac
     3301 acatgccgtt acggtgaatt atgaaatata tcacgtaagc ccaaaaacat ggcacatgca
     3361 tggtatgtga ctggtataaa actatcaaaa aatgatatat ccttatccgg attctttttg
     3421 aaatccagta taccagtagc acttatgaag taaatcttcc catacacaaa cattgagttc
     3481 tattgcacat gcacctgcca actctatcgc attcatgttc atcacaattc attggacggc
     3541 aagccataca gtgatcacag aatttcttat tcgggtcatt atggcatgct gagttaccgt
     3601 tgaaccaggt ttctacatta tgtaagttct gcatatttta ttcacttccg taatattctc
     3661 tcatttcgtt cgcgctttca attttaagtt ccagattctc aatgccatta tcgatctgtt
     3721 tttgtgttct gtttttagta tttttcattt ttttgccctc attttctatc tatgtgtgta
     3781 acttttgcac atatatataa ttggtataag tagtatatat aattttagca acatttgcac
     3841 aacaatgtta ggtttatatt attccagacg tggtcaggtc ttgaaattcc atgtatcatt
     3901 gatccagaaa acaaagtaat cgcatacggc ttaaaccaca aagtaaaata ctgccggatc
     3961 gtgcgaagaa agatcaatgg caagaacagg ttctaccttc aacttatctg cgaaggaaca
     4021 ccataccaaa acccaaaaaa caagattggg acagaagaga tcgggctgga tgtaggtgtg
     4081 ggtacaatag cgacagtagc ggatacaaaa gctactctga aacagttttg cagtgaatta
     4141 atccctgatc agaaaaagaa acgtaaattg caacgaaaac tggatcggtc acttagggca
     4201 accaatcccg ataattataa caaaaacgga acggtcaaga agggaaagaa attcaagaaa
     4261 tcaaatcggt ataaaacaat aagcaagaac ttggctgaaa cagatcttat cttagccgca
     4321 catcgaaaat cccttcatgg caaaggcata aacgaaataa tagcaatggg aacacgaatc
     4381 cagacagaaa aagtatcgta taaaggctgg caaaagcgat acggcaggtc aattaatatg
     4441 cgtgctccat ccatgttcat gaatagcctc aaaagaaaag ctgaaaatgc tggtggctac
     4501 ttatttgaat ttccaacaca aacgacaaaa ctatctcaga catgccatat ctgcgaacag
     4561 acggttaaga aacgccttag caagagatgg catatctgtt gtggcatcag gatgcagaga
     4621 gacttgtact ctgcatttct ggcaaaatgt gttggaaaag atacaagcgc ccttgaattt
     4681 gccagagcgg ttcgctcgtt aatcctatac gaattataag tgaggctatg gatgctgtaa
     4741 tcctcccgga tgagagccat agagagcttg tatgaacgtg aggaatctcc tagctttagc
     4801 cgtgagagag gttcaaaagg gataatgaga aatttaatta tttcgtggaa gatattctga
     4861 caggagaaaa taaaaaaatc aaaataacag aaattggttt cttgataatg atgctatcaa
     4921 gaaatttgtt attgaaatag tatggactgt cgtaaaaaaa atccaccagt atccagatat
     4981 atgggacgaa aaaaagggtc aaattcaaac cacaatattt ttatagggga aactcccttc
     5041 cgcttcctac ccatgaccca acttgagaac tcgtatattg gataatttcc agtatggtca
     5101 tgattcatgt aaatgaggtg aacaaaaatg aatctaagac aaccaaatgc gaatgaagcg
     5161 acgcagacct ataatcgctc aaagagcgta tccccgatgt cagggatatg cacacggtgc
     5221 cttgatggat gccgcgggaa ttgtgaaatc ttcaaatcct ccttcagggg aagagaagta
     5281 atatatcctg gtccttttgg taatataaca gccggcgcag ataagaacta ccccgtggat
     5341 tattcccatc taaacataca gggctatgct gtgggagcat ggggacttcc agatggagaa
     5401 gaacctaacc cggataccgc cctctttccc agagtcaata ccgagaccga atatggctgg
     5461 gacaaaaaag tcaggatgag tgttccgatt ttcacaggag
//
LOCUS       DATGBN010000407         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_227601, whole genome shotgun sequence.
ACCESSION   DATGBN010000407 DATGBN010000000
VERSION     DATGBN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227601"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VKL21_10200"
     CDS             <1..437
                     /locus_tag="VKL21_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMB46181.1"
                     /translation="NYDKADGGVNDVLAELLDLNHIIHLPLGRIGEIKPMKAHEFASV
                     IAKKLDTNVSYKGDGDIKKVMVVGGSGFRREYIDVAIEKGADALVSGEMRHDAQRYAD
                     RLCLFDATHYATEAPSMRKMCERLPVESAFIEDKPDVRVYCL"
     gene            570..1004
                     /locus_tag="VKL21_10205"
     CDS             570..1004
                     /locus_tag="VKL21_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMB46182.1"
                     /translation="MPKLVVVYLSTSGNTKAMADAIVEGAISRNVDALAANFHEAKME
                     DLKSADAIAIGSSTFHYKMLPPMEKFIDSLDKANVKGKTGAAFGSYGWSGEAPGMIAQ
                     KMRDIGIKVIDPVLRVQYAPSEKDLDECKRLGKDLANKIKKA"
     gene            1092..1595
                     /locus_tag="VKL21_10210"
     CDS             1092..1595
                     /locus_tag="VKL21_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HMB46183.1"
                     /translation="MWTIIQEKFKNHPAQEKVIRLLLERGFQIKLPGRVVSGGIEIPH
                     TQIANEIEVDRRVVDATCETISKNEKLMQIFQNVRTIPFLRDVAPLLGLGVIVISPDN
                     AARKGLLGAVATAVSEHGITIRQAVSDDPYLTENPQLTIITEGKASGDLLNSLTRIKG
                     VKSVTVY"
     gene            complement(1603..2568)
                     /locus_tag="VKL21_10215"
     CDS             complement(1603..2568)
                     /locus_tag="VKL21_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMB46184.1"
                     /translation="MVVKSVYSILIFIIPCNYKEVEAKAIPELKGLIIPDHTIMEEHS
                     IVVRGDVIVGNYAELGYGLIADMIVAGEHVKINGNVISQNDVRIDMWSDIQGEVRTKS
                     DAYLGEFVKISGKLFAAGNLDVGNNVKINGGYDVKGWLVVRKPLPVVMFIFLYMMALL
                     QLGSEEEVEKALQELFSDEMPDNKLMSIPNRAKIDLDTIRANTRATVGSYCRLLGNFR
                     SRSLLMGNHDTLFGSIRTSGNITIGKGCTIHGNLISKKGKVRVGRNSIILGKITADSI
                     LLHETSKTEGTLTAPNSVTIERDDLEGLGEIEIKLFYGFLLLEEV"
     gene            2567..2740
                     /locus_tag="VKL21_10220"
     CDS             2567..2740
                     /locus_tag="VKL21_10220"
                     /inference="COORDINATES: protein motif:HMM:NF021636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HMB46185.1"
                     /translation="MCELKVILKGKTIMEDVVRITQEKENIILQSLLGETKTVPGRIR
                     DINLTKQEAIIES"
     gene            2760..>3230
                     /locus_tag="VKL21_10225"
     CDS             2760..>3230
                     /locus_tag="VKL21_10225"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HMB46186.1"
                     /translation="MMKAKVAINGYGTIGKRVADAVAAQDDMEIAGVVKTRPSFEACI
                     AKEKGFDLYASAEENIEEFKNSKIEISGTLEDLLEKADIVIDATPGDTGEEYKKLYKK
                     HGVKAIWQGGEKHELTGFSFNSEANYSGGLGRDLARVVSCNTTGLVRILYALDNA"
BASE COUNT          993 a          656 c          731 g          850 t
ORIGIN      
        1 ctaattacga taaagcagac ggcggcgtga atgatgtgct tgctgaatta cttgatttga
       61 accatataat tcatcttcca cttggaagga tcggagagat aaaaccaatg aaggcgcacg
      121 aatttgcatc tgttatcgca aagaaacttg atacgaatgt cagctataaa ggagatggcg
      181 atatcaagaa ggttatggtt gtgggtggaa gcggattcag gcgcgaatat atcgatgtcg
      241 caatagagaa gggcgctgat gctcttgtat caggtgagat gcgccatgat gcccagcgat
      301 atgcagacag gttatgcctt tttgatgcaa ctcattatgc aacagaagcg ccttcaatga
      361 gaaaaatgtg tgagaggttg cctgtcgaat ctgcttttat tgaggataag cccgatgtca
      421 gggtatattg tttataggct gcaatattta ttcgtattta tgtgaacgaa aggtttatcc
      481 gagaataatt gcaacttaat tattagaatg ggatagtgtg aataagaaat cccatatctg
      541 aatgagaata agataaaggt gggaaaaaca tgccaaaatt agtagtcgta tatctaagca
      601 cctcaggtaa cacaaaagcg atggccgatg caatcgtcga aggcgcaata tcaagaaacg
      661 tcgatgctct tgccgcaaat ttccatgaag caaaaatgga agacttgaaa tccgctgatg
      721 cgatcgcgat cggctcatca acatttcatt ataagatgct ccctccgatg gaaaagttca
      781 tagacagttt agacaaggcc aacgtaaagg gaaaaacggg agcagctttc ggatcttacg
      841 gatggagcgg cgaagctccg gggatgatag cgcaaaagat gcgtgacatc gggataaagg
      901 tcattgatcc ggtgctgcga gtccagtatg cgccttctga aaaagacctc gacgaatgta
      961 agcgcctggg aaaggacctt gcaaataaga tcaaaaaagc ttaatcaaat tttgatctga
     1021 aatatttttt tttttgcttt ttttgagcca acccgataaa cattagtatc aaaatcacta
     1081 tattaatatt gatgtggact atcatccagg aaaaatttaa aaaccatccc gcccaggaaa
     1141 aagtcatccg attgcttttg gaaagaggat tccagataaa actcccgggt agagtagttt
     1201 caggcggtat tgaaatacct catacccaga tcgcaaatga aatagaagtg gacaggagag
     1261 tagttgatgc cacatgtgaa accatctcaa aaaatgagaa actgatgcag atattccaga
     1321 atgttcgcac catacctttc ttaagagacg tggcgccatt actcggcctt ggggtaatag
     1381 ttatttcgcc tgacaatgct gcccgcaagg gtctcctcgg tgcagtggca acagccgtgt
     1441 cagagcatgg gataaccatc aggcaggccg tatcggatga cccgtatctc acagaaaatc
     1501 cacagcttac aataataaca gaaggaaaag caagcggtga tcttttaaat tctcttacaa
     1561 gaataaaagg tgtaaaaagc gtgacagtct attagtttac tttcaaactt cttctagcag
     1621 caaaaatcca tagaacaact tgatctcaat ctcgcctaat ccttcaaggt catcccgttc
     1681 tatcgtaacg ctgttgggtg ccgtcaaagt tccttctgtt tttgaggtct catggagaag
     1741 gatagaatct gcggtaattt ttccaagaat tatactgttt cgtcccaccc tgacttttcc
     1801 tttttttgaa ataagatttc catggatggt gcagcccttg ccaatggtta tgttcccgga
     1861 agtgcggatg cttccaaaca acgtatcatg atttcccatt aacaatgacc ttgaacggaa
     1921 atttccgagc agacggcaat agcttcccac ggtagcccgc gtatttgccc tgatggtatc
     1981 aaggtctatc tttgccctgt tcgggatgga cataagtttg ttatccggca tttcatccga
     2041 gaaaagttcc tggagcgctt tctctacttc ttcttcgctg ccaagctgca gcagcgccat
     2101 catatataag aaaatgaaca ttactaccgg cagcggtttg cgcacaacaa gccatccttt
     2161 cacatcatag ccgccattga tctttacatt attacctaca tcaagattcc cggccgcaaa
     2221 gagttttcct gaaattttga caaattcccc gagatatgca tcagattttg tcctgacttc
     2281 accctggata tcagaccaca tatcgatgcg gacatcattc tgggaaatga cattgccatt
     2341 tatcttaaca tgctcgccag cgactatcat atcagctatc aggccatagc ccagttcggc
     2401 gtaatttcct acaattacat ctcccctgac cactatggag tgctcttcca ttatggtatg
     2461 atcgggaatt atgagtcctt tcagttcggg tatagctttt gcctccacct ctttatagtt
     2521 gcagggtatg ataaatatta atattgaata gaccgattta acaaccatgt gtgaactgaa
     2581 ggtaatcctt aaaggaaaaa ctatcatgga agatgttgtg cgaataacac aggaaaagga
     2641 aaatatcata ctccagagcc tgcttggtga aactaagacc gttcctggca ggatccggga
     2701 tatcaacctg acaaaacagg aagctataat agaaagctga ataattttat aaagggaaaa
     2761 tcatgaaggc aaaagtcgca ataaacggtt acgggacgat tggaaaacgt gtggcagatg
     2821 cagtggctgc acaggatgac atggaaatcg cgggcgtcgt taagacaaga ccaagttttg
     2881 aggcgtgcat cgcgaaggaa aaaggattcg acctgtatgc atcggcagaa gaaaatattg
     2941 aagaattcaa gaactcaaaa atcgaaatta gcggaactct tgaggacctt ctggaaaaag
     3001 cagacattgt tattgatgca acaccaggag atactggtga ggaatataag aaactctaca
     3061 aaaaacatgg tgtaaaggcc atctggcagg gcggagagaa gcatgaactt accggttttt
     3121 cttttaactc cgaggctaat tattccggtg ggctgggacg tgatcttgcg cgggtcgttt
     3181 cctgcaatac caccgggctt gtaaggattt tgtatgccct tgataatgca
//
LOCUS       DATGBN010000408         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_421898, whole genome shotgun sequence.
ACCESSION   DATGBN010000408 DATGBN010000000
VERSION     DATGBN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421898"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /gene="cofH"
                     /locus_tag="VKL21_10230"
     CDS             <1..943
                     /gene="cofH"
                     /locus_tag="VKL21_10230"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HMB46187.1"
                     /translation="KKVSVDRVTYVVNRNINFTNKCIGTCKFCAFKKQDGYIMSIPEI
                     LEKTRAAARINATEVCIQGGLLPDWDVFDYCSILENIKSEFPNIHIHAFSPMEVYHAS
                     QNSNMSYKETLSNLKRSGLGSMPGTAAEILVDRVREEICPDKLTTHEWIDVVKTAHNS
                     GLPTTATIMYGHIETLQERIEHIMLIRDIQKETGGFTEFVPLPFMSSNNVLGMGKSVS
                     RGIEDLKLHALARVLLYPYVKNIQASWVKLGRELAQSALTCGANDLGGTLMEEQISKS
                     AGATSGEYMAPFEFEGMIRQMNRIPQQRDTLYRQVNP"
     gene            1282..1656
                     /locus_tag="VKL21_10235"
     CDS             1282..1656
                     /locus_tag="VKL21_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator protein"
                     /protein_id="HMB46188.1"
                     /translation="MKSLSVNVLDKADEEFADTLIELGLKRNVAKVLTYLKNVKDVTS
                     RDLEMGSDLRQPEVSIAMRELEELAWIAEREEKKPGKGRPYKIYQLKTNIESIIEHLE
                     TQKKKESQAMMASIKRLKELTK"
     gene            1710..2795
                     /locus_tag="VKL21_10240"
     CDS             1710..2795
                     /locus_tag="VKL21_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HMB46189.1"
                     /translation="MHYHIILTKECNLKCNYCGGGSDSPPREIQYSIDDLKNFLSHDE
                     KPVIEFYGGEPLLRIGTMKKIMDRIPARFVIQTNGLLLYKLESHYLEKFHYILVSIDG
                     TKDVTDSARGNGVYDSIMDNIKLIRQRGFSGDLVARMTVAQETDIYENVMHLLTEKDG
                     FDNVHWQLDFEMFWEAWEHTVPGIPEWIHSYNSGISSLIRWWVSRMVRTGQVVGIVPF
                     IGVMKSLLKDTDSHLRCGSGIDFFTIMPDGRISACPVSIDFDFSVIGSIFEDIPSSLI
                     DKAKVGEPCISCEILRICGGRCLFVNRSQELLRENGYEMICSTVRHLVKELQETLPHI
                     RELIETGVIAGSDFDYPEFNNGCEIIP"
     gene            complement(2785..3243)
                     /locus_tag="VKL21_10245"
     CDS             complement(2785..3243)
                     /locus_tag="VKL21_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HMB46190.1"
                     /translation="MFESLESMLIGFGYQGLFLASFLASTILPFGSEGILIYLVQNKF
                     NIPALVLVASVGNFLGACTTYYIGFKGRTFVEKYLKFNPEELDKAEKFFTKYGSFVLL
                     FTWLPFLGDAITVVGGLLRLDFRIFSVFVFTGKFLRYLAVAYLTAAVFRV"
     gene            3346..>3566
                     /locus_tag="VKL21_10250"
     CDS             3346..>3566
                     /locus_tag="VKL21_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46191.1"
                     /translation="MEADEIIRILNQDFIREIEATLIYVRNAFIIPQCDPSRVTEAIA
                     VDEMRHMNWLADLIVRRGGKPSMEHKELD"
BASE COUNT         1115 a          655 c          797 g          999 t
ORIGIN      
        1 aaaaaaagta tcggtggatc gtgttaccta tgtcgttaac aggaacataa attttacaaa
       61 taagtgcatc ggtacctgca aattctgcgc gttcaagaaa caggatggat atatcatgtc
      121 aatccctgaa attcttgaaa agacaagagc tgctgccaga attaatgcca cagaggtatg
      181 tatccaggga ggtcttcttc ccgattggga cgtatttgat tattgcagta ttcttgaaaa
      241 tattaagagt gaattcccta acattcatat ccatgctttt tctccaatgg aagtatatca
      301 tgcttcgcaa aacagcaata tgagttataa agaaaccctc tcaaatctaa aaagatcggg
      361 tcttggctcc atgccgggaa ctgccgcaga aatacttgtt gaccgtgtca gggaagagat
      421 atgcccggat aaacttacta cccatgagtg gatagatgtc gtcaagactg cccataattc
      481 aggcctccct accactgcga caataatgta cgggcatatt gaaactcttc aagaacgaat
      541 agaacatata atgttaataa gggacatcca gaaagaaact ggcggtttca ctgaatttgt
      601 gccccttcct tttatgtcct ccaataacgt gctcggaatg gggaaaagcg tttctcgcgg
      661 catcgaggat ttaaaactcc atgctcttgc aagagtgctc ctgtatccgt atgttaaaaa
      721 tatccaggca agctgggtga aacttggcag agaacttgcg caatcagccc ttacatgcgg
      781 agcgaatgat cttggcggga cattgatgga agaacagatc tcaaagagcg ccggtgcaac
      841 aagcggcgag tatatggcgc cttttgagtt tgagggaatg ataaggcaaa tgaacaggat
      901 tccgcaacaa agggatactc tttacaggca ggttaatcct taactctgcc aaatgtcagg
      961 agcaaataat tgccgcagga agtcgaagtt ttttatgatg tttataattt ttgtaaatat
     1021 cagagtttaa atgttatagt taaatttatt tgacattatt atatttggat ttatttaata
     1081 caactaaatt aacgtttttt cgttttactt aaatatcatg tatataatta tgatgatttt
     1141 atagtaatga tgatgagtct acccatagtg agtctgcttt tttttttgca gcctttaatt
     1201 ataaatactt ttcttatata caatatatta aaacggaaga tttatattta atgactacat
     1261 ttaaagatac ggtgaaatgt tatgaaaagt ctttcagtca atgttcttga caaagcagat
     1321 gaagaatttg ccgacacgct tattgagtta ggattgaaac gaaacgttgc aaaggtgctc
     1381 acttatttga aaaacgttaa ggatgtaact tccagggacc ttgaaatggg ttccgatcta
     1441 agacaacccg aggtcagcat agctatgcgc gaacttgagg aactggcctg gatagccgaa
     1501 agagaggaga aaaaacctgg aaaaggcaga ccatataaga tatatcaact caaaaccaat
     1561 atcgaatcca tcattgaaca tcttgaaacg cagaagaaaa aagaatcaca ggcaatgatg
     1621 gcaagcataa aacgtttaaa ggaattgact aagtgaaata atttatattt tcaggggtgg
     1681 gaatccttta gttttatttt tgccataaga tgcattatca tataatcctg acaaaagaat
     1741 gcaaccttaa atgcaattat tgcgggggag ggagcgattc accaccccgg gaaatccagt
     1801 attctatcga tgaccttaag aatttcctct cccatgatga aaaacccgtt atagaattct
     1861 atggggggga gcctcttctt cgtattggga ccatgaaaaa gatcatggac cggatacctg
     1921 cgagatttgt tattcagacc aacggccttc tgctttataa actcgaatct cattatttgg
     1981 aaaagttcca ttatatcctt gtatctattg atgggacaaa ggatgtgaca gactcagcgc
     2041 gtggaaatgg ggtctatgat agcatcatgg ataatataaa gctgatcagg caaaggggat
     2101 tttcaggaga ccttgtggcc aggatgacag tagcgcagga gacggatatt tatgagaatg
     2161 taatgcatct tctcactgaa aaagatgggt ttgacaatgt tcactggcag cttgattttg
     2221 aaatgttctg ggaagcatgg gagcatacag tccctggtat tcctgaatgg atccactctt
     2281 ataattccgg gatctcctca cttataagat ggtgggtttc caggatggtg cgaacaggtc
     2341 aggttgtcgg gatcgtacca tttattggtg tgatgaagtc tcttcttaaa gacactgact
     2401 cacacctgcg gtgcggctca ggaattgatt tttttacaat aatgccggac ggcaggattt
     2461 ctgcttgtcc tgtttctatt gattttgatt tttccgtcat aggctcaata tttgaagata
     2521 ttccgtcatc tctgattgat aaagcaaaag ttggcgaacc ctgcatatcc tgtgaaattt
     2581 tgcggatttg cggggggaga tgcctttttg tgaaccggtc acaggagctg ttaagagaaa
     2641 atggatatga aatgatatgc agcacagtcc ggcatcttgt aaaggaattg caggagactt
     2701 tgccgcatat ccgggagctt atcgaaaccg gcgtaattgc aggatcggac tttgattatc
     2761 cggaattcaa taacgggtgc gagatcatac cctaaaaaca gccgctgtaa gatatgccac
     2821 tgccaggtag cgaaggaatt tgcccgtgaa tacaaagacc gaaaagatcc gaaaatcaag
     2881 gcgcaataat ccgcctacga ccgtaattgc gtctcccagg aagggaagcc atgtaaataa
     2941 tagcacaaag gagccgtatt tggtgaaaaa tttttcggct ttgtcaagtt cctcgggatt
     3001 gaatttgaga tatttttcaa caaatgttct tcccttgaat ccgatgtaat aagttgtgca
     3061 ggctcccagg aagttgccca ccgatgctac taagaccagt gcagggatgt taaatttatt
     3121 ttggacaaga taaatcaata taccttcgct tccgaacggg agaatggtgg aggccaggaa
     3181 acttgccagg aaaagaccct gataaccaaa cccgatcaac atgctctcta aagattcaaa
     3241 catggaccga aataatcaca cctgatataa gaacaatcgt atttaattaa tgataaatac
     3301 tcctgtgttg ttcattattt aatatttttc cagggaggac ttgatttgga agctgatgaa
     3361 ataatccgta tattgaacca ggatttcata agggaaattg aagccacgtt gatctatgtc
     3421 aggaatgctt tcatcatacc gcaatgcgat cccagcaggg tgactgaagc gatagctgta
     3481 gatgaaatga ggcatatgaa ctggcttgca gacctcatag taagacgggg agggaagccg
     3541 agcatggaac acaaggagct tgattt
//
LOCUS       DATGBN010000409         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_499179, whole genome shotgun sequence.
ACCESSION   DATGBN010000409 DATGBN010000000
VERSION     DATGBN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499179"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..372
                     /locus_tag="VKL21_10255"
     CDS             136..372
                     /locus_tag="VKL21_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46192.1"
                     /translation="MSFKTIKIKNETYESINVLVGDLMKELKRPVSMDEALRFLLKSR
                     KNKPSKFAGGWKMDEDEFEKIKRGLKETWKRWEL"
     gene            360..752
                     /locus_tag="VKL21_10260"
     CDS             360..752
                     /locus_tag="VKL21_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMB46193.1"
                     /translation="MGIVIDTDILIDLLRNKTYAIKKIKELERNEELATTDINAFEIY
                     FGAYNSRDRERNILSAKGLLRNLTIFHTEEDSMETAGRIFAERRARGKMIEIRDLLIA
                     AITLQNGCRLLTNNRGHFEGIEGLSLEI"
     gene            complement(883..1302)
                     /locus_tag="VKL21_10265"
     CDS             complement(883..1302)
                     /locus_tag="VKL21_10265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMB46194.1"
                     /translation="MNLRGTRKKMTVLVDSWSWIEYFKGSAAGEKARESIENSQEKVI
                     VSAVNIAEVYNSFLRDYSPPNNKRYAEASRKAMKQRSYVYEVDEEIAVDSAKIKHEKK
                     WGLGYSIIYATAKREGAKVLTGDPHFRGLKDVIFLER"
     gene            complement(1259..1396)
                     /locus_tag="VKL21_10270"
     CDS             complement(1259..1396)
                     /locus_tag="VKL21_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46195.1"
                     /translation="MNLKSYEEVIRALLRSEKRLNKSYFGMFPELYEFKRDEEEDDRL
                     G"
     gene            complement(1393..3747)
                     /locus_tag="VKL21_10275"
     CDS             complement(1393..3747)
                     /locus_tag="VKL21_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HMB46196.1"
                     /translation="MIKPELLAPVGGKEALIAAIENGADAVYFGGALFSARQYASNFT
                     NEEIESAIDYSHIRGVKAYVTMNTLVKDSELEKAGQYLQFLCNAGADAVIVQDIGILR
                     LLREQLEILPVHASTQMTIHNLEGVKFLQEMGVKRVVLARESSLGEIRNIKSQTSVEI
                     ETFIHGAICFSYSGQCLLSSMIGGRSGNRGYCAQPCRKKYRIGDLEGYLLSPKDLNIS
                     AHIGALLDAGIDSFKIEGRMKRPEYVAGVVRVYRRLIDRYLAAPGEFRVTKSEIHTLL
                     QLFNREFTTGYFLGNPGSQLMSRKYPHNRGTELGKTVEYDTRTKLVTIDLKSSLRIGD
                     GIGIGNRETGITVRNIYIGNKMAKEAAPGSLIRIPLDIEVSEDEEVFKTFDSKLMASL
                     ETGRINKIPIKMSFKARIGVPLGLLIDDGENKITISRDKVISAKTKPASKSSIAQHLI
                     KLGGTIFETKEVDFELDKNIFIPVSELNAIRRQGIEELEKIRARKWKRQCSEPKIFPG
                     YGKFESKPILAVNTRTIGSFKAAVDSGADVVYIGEEVFENHEAIESAHKKGVKIFIST
                     PRIIKDLKELEIFDTEQTANGPIKEADGFLVGNPGIFHHLRTNLANKPIVIDYPLNVF
                     NRLTMDHFLNSSLRVTLSPELTLNEIRNLVLSGPSECIVHGFFPLMVSEHDLIGGLFP
                     GRSSGDIFLKDEKGFKFPVMTDIHNRTYVMNSRELCLIESVPEIIKAGVSCLRVEART
                     YDAKMTGEITTEYRKAIDDAVCGRESGKRCAGEHTTGHYFRGVL"
     gene            3894..4214
                     /locus_tag="VKL21_10280"
     CDS             3894..4214
                     /locus_tag="VKL21_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46197.1"
                     /translation="MDTKDISLAVIMTLSSVVLTIRWLTRLGNVSDPVIILSAVLLIG
                     SLAGMILLLDNRLRNIEEALDVKERALRINIQGVESHLDKKMDVFVQKTEDKIGEFSK
                     RIYR"
     gene            complement(4195..4497)
                     /locus_tag="VKL21_10285"
     CDS             complement(4195..4497)
                     /locus_tag="VKL21_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46198.1"
                     /translation="MDIRLSRPCVDDPTRYIAECHLGKRLVMEKLCDILRQIGAKDLK
                     CSLNLGVARFELEEKSVMLYQSGRVDIRKIRNTEEARIFLEKIFSMVREALSDISS"
BASE COUNT         1355 a         1035 c          942 g         1419 t
ORIGIN      
        1 gcaatgataa gtgagaaatg cacagggtgc ggaaaatgca catggacatg ccctgtagtg
       61 aaattcggat aattcaacct gaaatagaac gaaactatat attctttacg aaacaaagta
      121 gataaagtgg taaaagtgtc atttaagacg ataaaaataa aaaatgaaac atatgagagt
      181 ataaatgtat tagttggcga tttgatgaaa gaactgaaaa gaccagtgtc catggatgag
      241 gcattaaggt ttttgcttaa aagtagaaaa aataaaccaa gcaagttcgc aggaggctgg
      301 aaaatggacg aagatgaatt cgaaaagatc aaacgcggat taaaggaaac ctggaagaga
      361 tgggaattgt aatagatact gacatattga tcgacttgct cagaaataag acatatgcga
      421 ttaaaaaaat aaaagaactt gaaagaaatg aagagctagc aaccacagat ataaatgcct
      481 ttgagatata tttcggcgct tataattcca gagacaggga gaggaatatt ttatctgcaa
      541 aagggctgct tagaaatctg actatatttc acaccgagga agatagcatg gaaacagcag
      601 ggcggatttt tgcagaacga agagccaggg ggaaaatgat tgaaataagg gacttactaa
      661 ttgctgcgat tactttgcag aatggatgca ggcttcttac caataataga gggcattttg
      721 aagggataga aggtctttct ctagagatat aagaataatt agaaagaaat cggtagtaag
      781 aaatcagatg gagggattgg cgacggtgaa ttggattttc ttatatctat tttcgatttt
      841 gctgaattta acaaaatcgt ttacattctc ctaaatttcc tttcaccttt ccaggaatat
      901 gacatctttc agacccctga aatgtgggtc tccggtgaga acctttgcgc cttctctttt
      961 tgcagtcgca tatattatag aataaccaag gccccatttc ttttcatgct ttatttttgc
     1021 agaatcaaca gcaatctctt catcaacctc atagacatat gatctttgtt tcatggcctt
     1081 ccttgaagcc tctgcatagc gcttgttatt cggaggagaa taatcccgca gaaatgagtt
     1141 gtacacttcc gcaatattta ccgcacttac aattaccttt tcctgtgagt tttctattga
     1201 ttccctggcc ttctcgccag ctgcgcttcc tttgaagtac tcaatccatg accaggaatc
     1261 aaccaagacg gtcatcttct tcctcgtccc tcttaaattc ataaagctca gggaacatcc
     1321 caaaatatga tttattaagc ctcttctctg atctgagaag tgcccggata acctcttcat
     1381 atgatttgag gttcataaaa ctcccctaaa ataatgtcct gtggtatgct ccccagcaca
     1441 acgtttgcca ctctccctgc cgcaaacagc atcatcaatt gccttcctgt actctgtcgt
     1501 aatttcccca gtcatctttg catcgtatgt tcttgcttct accctcaagc agctcacacc
     1561 tgcttttatg atttccggca cggattcaat caggcataat tcacgggaat tcataacata
     1621 agtgcggtta tgaatgtctg tcatgaccgg gaatttaaaa cctttttcat ctttcaggaa
     1681 tatatccccc gagcttctgc caggaaaaag acctccgata agatcatgct cggataccat
     1741 cagcgggaaa aagccgtgaa cgatgcattc agaaggtcct gacaagacaa ggtttcttat
     1801 ttcattcagg gtaagttcag gagataatgt cactctcagg ctggaattca agaaatgatc
     1861 cattgtcagc ctgttaaaga cgtttagtgg ataatcaata actattggct tattggcgag
     1921 atttgttcga agatgatgga aaatacctgg attccccaca aggaaaccat ctgcctcttt
     1981 aattggacca ttagcagtct gttcagtatc aaaaatctca agctctttta aatcctttat
     2041 tattcgcggg gtgctgatga atatcttaac acctttttta tgcgcggatt caatagcttc
     2101 atgattctca aatacttctt ccccgatata aacaacatca gcgccgctat caacagcagc
     2161 tttgaaagat ccaatagttc tagtatttac cgccaggatg ggcttgctct cgaatttccc
     2221 gtatccgggg aatatctttg gttcactgca ttgtcttttc catttccgtg cgcgtatttt
     2281 ttcaagttct tctatcccct gccttcggat cgcgttcaat tcggaaacag gaatgaaaat
     2341 attcttatcc agttcaaaat ccacttcctt tgtctcaaaa atcgtgcccc ctagctttat
     2401 taaatgctgt gcgatcgaac tcttggaagc gggttttgtc tttgccgaaa tgactttatc
     2461 cctgctgatc gtgatcttat tttctccatc atcgatcaaa agcccaagcg gtacaccaat
     2521 tcttgcttta aatgacattt ttatcggaat tttattgatg cgcccggttt ccagtgatgc
     2581 catgagcttg ctgtcaaatg tcttaaagac ctcttcatcc tctgaaacct cgatatcaag
     2641 aggtatcctg ataagagaac cgggagctgc ctctttggcc attttgttgc caatataaat
     2701 attcctgaca gtgatccctg tttcccggtt ccctatacct attccatccc cgattctcaa
     2761 agaagacttt agatcaatcg taaccaattt tgtccgtgta tcatattcaa ccgtttttcc
     2821 aagctctgtc ccgcggttat gcgggtattt ccggctcatt aactggctgc ccggattccc
     2881 caaaaagtat cctgttgtga attccctgtt gaacaattga agaagcgtgt gtatttcaga
     2941 ttttgtgacc cggaattccc cgggcgcagc cagatacctg tcgataagtc ttctataaac
     3001 ccgcaccacc cctgcaacat attccgggcg cttcatcctc ccttctatct tgaaggagtc
     3061 aatacctgca tccagtagcg cgccgatatg cgcgcttatg tttaggtctt ttgggcttaa
     3121 gagatatccc tccagatcac cgatcctgta ttttttccgg catggctggg cgcaatatcc
     3181 acggttcccg cttcttcctc ctatcatact gctcaggagg cactggcccg aatatgaaaa
     3241 acagatcgcg ccatgtataa atgtctctat ttcgacagac gtttgcgatt tgatattccg
     3301 gatttcgccc agggaacttt cgcgagccag gacaacgcgc ttaacaccca tttcttgaag
     3361 gaacttaact ccctccagat tatggatggt catctgggtg ctggcatgga cgggaagaat
     3421 ttcaagctgc tccctcagca aacgaaggat gccaatatcc tgcactatga ccgcgtcggc
     3481 gccggcattg cacaggaatt gtagatattg gcctgctttt tcaagttcgg aatcctttac
     3541 cagtgtattc atggttacat aggctttgac acctcgaata tgcgaataat cgatcgccga
     3601 ttctatttct tcattcgtga aatttgatgc atattgccgg gcgctaaaaa gcgccccccc
     3661 aaaataaaca gcatcagctc cgttctcgat ggctgctatt agagcttcct tgcccccgac
     3721 cggagccagt aattctggtt tgatcatata ttttgtatta taatgttcgg aggttaaaaa
     3781 gtagttgcac tattttggcc aattcacact ttttgaactt ttttaatgga ctctacaact
     3841 aaatatatat atatgcaaaa gaataaccac tatggaaaat cagaggtgga tgaatggaca
     3901 ctaaggatat ctcacttgca gtgattatga cactgtcttc agttgtattg actattagat
     3961 ggctgacacg tctcggtaat gtttccgatc cggtaatcat attgtcagcg gttctcttga
     4021 taggttctct ggcaggaatg atattgctgc ttgacaatag attaagaaac atagaggaag
     4081 cccttgatgt caaggagcgg gcattaagga taaacataca gggagtagaa tctcatcttg
     4141 ataaaaaaat ggatgtattt gtccagaaaa cagaagataa gatcggtgaa ttctctaaga
     4201 ggatatatcg ctgagcgctt ccctgaccat tgaaaatatt ttttcaagga agatccgtgc
     4261 ttcttctgtg ttacggatct tcctgatatc aacccgtcca ctctggtata acataacgct
     4321 cttttcttcg agttcgaaac gggcaacgcc aagatttaac gagcatttta aatccttagc
     4381 tccaatctgg cgcaagatat cacataattt ttccatgacc aatctttttc caagatggca
     4441 ttctgcaata tatcgggtag ggtcatcaac gcacgggcgg gaaagccgga tatccatgat
     4501 gtgtgtaata gttgatagga atataaaaac tccgtccaaa aatatcgcat tcatgaaact
     4561 attatctatt gttctcgaaa tcggtatgga aaaaattatt aatcgcagat gcataatgcc
     4621 ataattctat ctgcaactgc gattatttct ttctttactc atccagaatt gcttctgccg
     4681 tttcccgata tgcgttcatt acatatggaa tgccggaaac ctttgaagcg tcctctgtca
     4741 gtgccattac a
//
LOCUS       DATGBN010000410         3461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_577203, whole genome shotgun sequence.
ACCESSION   DATGBN010000410 DATGBN010000000
VERSION     DATGBN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577203"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VKL21_10290"
     CDS             complement(<1..359)
                     /locus_tag="VKL21_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMB46199.1"
                     /translation="MVLSGSWQLEGCIKKLREKLDTSIRVMNSKSENSKGFKKELTFF
                     DLTNIIVGSIVGADIYIASALTAGMIGPFSIIIWVVAGIFATIIALVFAYCSYYVPRV
                     GGPFAFVSYAFDDFYGFL"
     gene            complement(370..1329)
                     /locus_tag="VKL21_10295"
     CDS             complement(370..1329)
                     /locus_tag="VKL21_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMB46200.1"
                     /translation="MTSFDEMVTGAVEKVIPSVVNISEVKLMKDAYLHMHPVQGVGSG
                     FIINEGGFILTNAHVVLGSQEIKVALEDGRIFPGEIRGLDTMKDLAIIKIKASGLPVP
                     DISKNNNLKIGQMAIAIGSPLGLVGGPTVTAGVISALNRSIQTEYTFMEGLIQTDAAI
                     NPGNSGGPLINSSGVVIGINSAIIPFAQGIGFSIPIQPAMWVAQQLIEHGEIVRPWIS
                     FNAIDVNPKLVAYYDLPTEKGVVVTNVIPGSEADKSGIEVADILVRMEDIEINNVRDL
                     IKVLNEHKVGDRVTMEFFRGPGKVKIKTVLETAPTSQRPPMAK"
     gene            complement(1406..2524)
                     /locus_tag="VKL21_10300"
     CDS             complement(1406..2524)
                     /locus_tag="VKL21_10300"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HMB46201.1"
                     /translation="MLVDLKDMEIKKLSEIYDVESRESFVSLYMNMEKTSERFVEKRK
                     MACRSVLKENRELLENFDKTMLKIEKYLSINDREQGQKGLAIFASNEHDFFKAYKLGM
                     PVEDLMVVNTSPYIRPIVKLIEDYETLGLVILDNHRARIYVVSSGRIADKNKIAKDIM
                     KNHKKGGMSQARFQRLRRGAIEHFMKEVSEEMVRLFSKDNVFKIVIAGPGNAKILLMD
                     FLPNQLKREILELIDVDFDEADGYLISRAEEAVLKDEKKTVSKNVTRLKEEILKHSLA
                     VYGLNDIIDAVKNGHIELLLVSKGYELKGWICEKCQLLDSGLIDKCPNCNGKVSQVDV
                     IEEIIDFAKRTDTKVEFVDDNPILHELGGVGGLLRFKI"
     gene            2729..3178
                     /locus_tag="VKL21_10305"
     CDS             2729..3178
                     /locus_tag="VKL21_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46202.1"
                     /translation="MKKTLFIGLLVIAVLISGCTTTTTTQPAPTQTPETTTPTPTPTP
                     AIVTPAPTPELTPFVAATPTGKNVTIIVKDDKYSPADITINAGDMVLWVNKMDSDYTL
                     IEMDKKIPDMVLRARNSYIFNATGNYRIGLYFKPMRGEPAILTINVR"
     gene            3247..>3461
                     /locus_tag="VKL21_10310"
     CDS             3247..>3461
                     /locus_tag="VKL21_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46203.1"
                     /translation="MPLKPVSELVAVMHWKKENVECDLYDVKENIPTKSPDPGRIVSD
                     SFNSPTPIGLLMKYDPFRILDYFLLIKP"
BASE COUNT          965 a          848 c          598 g         1050 t
ORIGIN      
        1 aggaaaccat aaaaatcatc aaatgcataa gaaacaaaag cgaaaggacc tcctactctc
       61 ggaacgtaat aactgcaata tgcaaaaacc agcgctatta ttgtagcaaa tatacctgca
      121 acaacccaga tgattattga aaaaggtcca atcattccag ctgtcaatgc agaggctata
      181 taaatatcgg ccccaacaat tgaaccgaca ataatattag ttaaatcgaa gaaagtcaat
      241 tctttcttga atccctttga attctcactc ttcgaattca taactctaat tgaagtatct
      301 aatttttctc taagtttctt tatacatcct tctagttgcc aacttccact caaaaccaaa
      361 aaaatttttt catttcgcca tcggcggacg ctgagaagtt ggagccgtct caagcacggt
      421 cttgatcttc acttttcccg gccccctgaa aaattccata gttaccctgt cgcccacttt
      481 gtgttcgttt aggactttga tcagatccct gacattattg atctcaatat cctccatgcg
      541 gacaaggatg tccgcaactt cgatgcctga tttgtccgct tcgcttcctg ggatcacatt
      601 ggtcaccaca accccttttt ccgttggaag atcatagtac gccacaagct ttggattgac
      661 atctatggca ttgaaactga tccacggtct tacgatttcc ccatgttcta tcagttgctg
      721 cgctacccac attgctggct gtatcgggat ggagaaacca atgccctggg caaaaggtat
      781 gatggcgctg ttaatcccta tcacaacacc actgctgttt atcaatgggc cgccgctgtt
      841 gccgggattg atggctgcat ctgtctgtat caatccttcc atgaacgtgt attcagtctg
      901 gatagagcgg ttcaatgcgc ttattacgcc tgctgttaca gtcgggccac ccacaaggcc
      961 aaggggactt cctatagcga tagccatctg cccgattttg agattgttat tttttgaaat
     1021 atccggtact ggcaggccgc tggctttgat cttgattatt gcaagatcct tcatggtgtc
     1081 caggcctctt atttcgccag ggaatatcct tccgtcttcc aaagccactt ttatctcctg
     1141 tgaacccagc accacatggg cattggtgag tataaagcca ccctcattta ttataaaacc
     1201 tgaacccaca ccctgaaccg gatgcatatg cagataagca tctttcatca gctttacttc
     1261 gctgatgttt acaacactcg gtataacttt ttctacagcg ccagtaacca tttcatcaaa
     1321 acttgtcata tttattgccc ctttaaacag attaattctc ttaatatttg aagctattta
     1381 tcgcttcatc ttataccgat agttatcata tcttgaaacg aagcaaacca ccaacgccac
     1441 ccagctcgtg caatattggg ttatcatcaa cgaactcgac ctttgtgtct gtccgtttcg
     1501 cgaaatcgat gatctcttct atcacatcga cctgcgacac tttaccgttg cagtttggac
     1561 atttatcaat taaaccggaa tccaaaagct ggcatttctc gcatatccag cccttcagtt
     1621 cataaccttt gcttaccagc agcaattcta tatgaccatt tttcaccgca tcgattatgt
     1681 cattcagacc atagaccgca aggctgtgct tcagaatctc ctcctttaat cgagtcacat
     1741 tcttcgaaac cgtctttttt tcatctttca ggactgcttc ctcagccctt gaaataagat
     1801 acccatcggc ttcatcaaaa tccacatcga tcagctccag gatttcacgc ttaagctggt
     1861 tcggaagaaa atccatgagt aatatcttcg cgttgccagg accagcaatg acgatcttga
     1921 aaacattatc tttcgaaaat agtcttacca tttcctccga gacttccttc ataaaatgct
     1981 ctatagcgcc ccgtctcaac ctctggaaac gcgcctgcga cataccgcct tttttatgat
     2041 ttttcatgat atccttggcg atcttgtttt tatccgcaat cctgcccgag gaaactacgt
     2101 atattcttgc ccggtgattg tccagaatga ccagtccgag tgtttcatag tcttctatca
     2161 attttacaat tggccgtata tacggcgatg tattcacgac catcaggtct tcaactggca
     2221 tccccaattt gtatgcttta aaaaaatcgt gttcgttcga tgcgaatatc gcaagccctt
     2281 tctgcccctg ctccctatcg tttatactca aatatttctc gatctttagc atcgtcttat
     2341 cgaaattctc caacaactct ctgttttctt ttaatacgga tctgcatgcc atttttcgtt
     2401 tctcaacaaa tctctcgctc gttttctcca tgttcatata aaggctaacg aacgattctc
     2461 tgctctcaac gtcatagatt tccgagagtt tcttaatttc catatctttc aggtcaacaa
     2521 gcatgagctt gagaagaggc tttcaatatt tatacttcac tttcgcttta tttattacct
     2581 taaacaaaat gttattatct gattttagta ttgtattttc catttggaaa tagatgtttc
     2641 gggacatcaa agaatcatcc aaaaatgata aatagtaagt aaattataca ttctacacgg
     2701 gattatatat cctacatggg aggttaacat gaaaaagaca ttgtttattg gactgttagt
     2761 aatcgctgta ttaataagtg ggtgtaccac aacaactacg acgcaacctg caccaacgca
     2821 gacaccggaa acaacgactc caacgcccac accaactccg gcaatagtga ccccggcacc
     2881 cactccagaa ctaacacctt ttgtcgcggc aactcctaca ggaaagaatg taacaattat
     2941 tgttaaggat gacaagtact ctcccgcaga tatcactata aatgcgggag atatggttct
     3001 ctgggtgaat aagatggata gcgactatac acttattgaa atggataaga agatacccga
     3061 catggttctc cgtgcaagaa atagctatat tttcaatgcc accgggaatt atagaattgg
     3121 gttgtatttc aaaccgatgc gtggtgagcc ggcgatcctg accataaacg taagataatg
     3181 agaacaatgc aggcaattaa cttgcattct tcacaatttt tttgacaatc tttttcactc
     3241 ttagcaatgc ctctcaagcc tgtatctgaa cttgtagcgg tcatgcattg gaagaaagaa
     3301 aatgttgaat gtgacctcta tgatgtgaaa gaaaacatcc caacaaaaag tcctgatcct
     3361 ggaagaatcg tgtcagatag tttcaattca ccaacaccaa ttggtctgct aatgaagtac
     3421 gatccttttc gcatcctcga ttattttctc ctcatcaagc c
//
LOCUS       DATGBN010000411         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_72302, whole genome shotgun sequence.
ACCESSION   DATGBN010000411 DATGBN010000000
VERSION     DATGBN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72302"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..665)
                     /locus_tag="VKL21_10315"
     CDS             complement(81..665)
                     /locus_tag="VKL21_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46204.1"
                     /translation="MNALFETDINTLKNISAVNVISHSEVKELCKVENDTTPERTLGC
                     FDPKNYNAYVSELLTYSSLTCGTFENTLYHEIGHADYLSKNAYVSYLEVPYMERPPEI
                     YAFKYADQHSKDKCNSDKYIELNNLLNVSEKAEKLAGSQLSYWDRYGTSIPEDVYFAY
                     MRDYNTHLQAINKYNSIVKEIDEYMNNTLLTTRI"
     gene            753..1451
                     /locus_tag="VKL21_10320"
     CDS             753..1451
                     /locus_tag="VKL21_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMB46205.1"
                     /translation="MSGKFFYILGNIRSMRFDLHIHSKYSSDSRLAIEDILRKAVQRG
                     LDGIAICDHNTVAGSYHARKFVTKLNLPLLVLPGVEVSTTQGHLIVLGVKDNIPSGMT
                     PQETIKIARQKGGVVIAPHPFKIRSIGNVDGLDIDAIETFNSRCIFGENEKARKMAIE
                     LGKPQVGGSDSHMLGTIGLGYTEIDAQPYEASVLNAIRKGNTCPGGKNAPIYIIILQV
                     FRGVLRRLRTSRSK"
     gene            1510..2919
                     /locus_tag="VKL21_10325"
     CDS             1510..2919
                     /locus_tag="VKL21_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMB46206.1"
                     /translation="MEKFDVIIVGGGISGLLSALTLSKHGKKVLLLEKDDAVGGNCNS
                     YMVDGFQVDTGPHAITHLREGPLRRLMDEYFTYMPFFVDYGHYYVRTEKGLTKIPSNI
                     KDFVTFDVLPRKDRLMLSQALTKALTQMIFGMLDGDQPVYDYLPKGLSGDTYDFVNAI
                     AYFLSGKSMKDTSVNRVLYGSSFVRDSVSVDIFEDVEVESGTSYTSLITDKLSKYQLK
                     GHLTSLSRLATNKVAYAQAYPRGGLRSLLNAVLYSMPETVQIKTGSRVSRILMDGEKA
                     IGVATDSDSYSSDIVVYTGFVKSLPCLVPLPSDYVKKLSTIDQTLSLTIWLGLKGKMK
                     EFDYTGSEVWFRGGAYWAMPISNYDASIAPKGKQLVGFTFVIDEKNDIDSEKRKAMGL
                     IIKAVPGIEKMVEMTHYQVTIPEKAAVTINGYFAGPRSPIRNLYLAGTDTDSRSMGVT
                     RAAYSVLELLKAMKEDKIL"
     gene            complement(2934..3251)
                     /locus_tag="VKL21_10330"
     CDS             complement(2934..3251)
                     /locus_tag="VKL21_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46207.1"
                     /translation="MNKRIALLAVLGSIFLFSGIYFGNIAFVYLGVGTYLSGLIYFTF
                     FPRFKEITSIILGLLVLHTGVLLLLKENLSNTKLLGFLIFTIGIVFVLNSGFSDYMKN
                     RKK"
     gene            complement(3280..3618)
                     /locus_tag="VKL21_10335"
     CDS             complement(3280..3618)
                     /locus_tag="VKL21_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46208.1"
                     /translation="MEIKNIKGIGYGLLTFGLLFILFSVYSMYSVYTGAEAAPSVIQM
                     NSVKLSLPTGPGTPPMETELMSGKDSSTMTNMGLWFMLMTFVASAGGRIGGLGIKLVR
                     DIKVEVKNED"
     gene            3920..4795
                     /locus_tag="VKL21_10340"
     CDS             3920..4795
                     /locus_tag="VKL21_10340"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMB46209.1"
                     /translation="MKNTDPKLSELILFFAENGQEIKNGFLKSQNKAGTSNIYGEEQM
                     ALDKWADEVLINGLKKTRLVRYIATEEQPGIIEIDNPRNQFGVVIDPLDGSSLIDVNL
                     AVGTIIGIYPGTVLDKGENMIAAMYILYGPLTTLTLTTGNGVHDFVMGEKGEFYLTAE
                     NIKIPDGKIYAPGALRKDYLPAHSKFIESLENEGYKLRFSGSFVADMHQILHKGGVFT
                     YPGFKGKEKGKLRLLFEANPMGRIIAEAGGAISNGKKDILSILPGAIDNITPVYAGSK
                     KEIGLIERYTQELSV"
     gene            complement(4842..5807)
                     /locus_tag="VKL21_10345"
     CDS             complement(4842..5807)
                     /locus_tag="VKL21_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMB46210.1"
                     /translation="MNKESLINQAFVILSKAGFIISERCDIRPRSFDIAVRRDNLLLL
                     IKVISNIDGLNEETTREMQFLSKQLHGYPIVIGEKTRDHSLETGVVYFRYGVPAFDIR
                     TMQDYFVEDLPPLIYAEHGGLYVNIEGNILKEERIKKNISLGALASMLGVSRRTISKY
                     EEGEMAASVDVAVKLEEVMDRGFTVSVNLFEKLHRDDAQKTETVPEHSNVFSILQDMG
                     FNVMPISQAPFDAVSQSFNRKDEDATILTGVAQYSRTMVKKAHLMSSISEVAQTQSLL
                     IIQGAGKTKNIEGTVIIDNKELEKFNDKDDFIELLQERRRKIKVS"
BASE COUNT         1837 a         1147 c         1226 g         1775 t
ORIGIN      
        1 tgaaataatt ggagatgcgg tattaaaatt caatgaaatg atagatgcga aaattattga
       61 ataaatacta tgtattttat ttatattctt gttgttaaca aagtattatt catgtattca
      121 tctatttctt ttacaataga attgtattta tttatagcct ggagatgtgt gttataatct
      181 cgcatatatg caaaatatac atcttccgga atggatgttc catatctatc ccaatatgat
      241 aattgcgatc ctgctaattt ttcagctttt tctgaaacat ttaacaaatt attcaactct
      301 atgtatttat cagaattaca tttatctttt gaatgttgat cggcatattt aaatgcatat
      361 atttcaggtg gtctttccat atatggaact tctaaataac taacatatgc atttttggat
      421 aaataatcag catgcccgat ttcatgatat aatgtatttt caaatgttcc acatgttaat
      481 gaactatatg ttaacaactc tgatacatag gcattgtaat tttttgggtc gaaacaacct
      541 aatgttcttt ctggcgttgt atcattttct actttgcaaa gttctttaac ctcagagtga
      601 cttataacat ttactgcgga tatattttta agagtattaa tatctgtttc aaacagagca
      661 ttcaaggttc gtatgagatt tacatatacg ttctctttga tgacaaaatg acctatatat
      721 atatccataa aaatatatgc tcataacttg aaatgtcagg taagtttttt tacatcctgg
      781 gaaatatcag gagtatgcgt tttgacctgc acatccattc aaaatattcc tctgacagca
      841 gattggcaat tgaggatata ttaagaaaag ccgtgcaaag aggactggat gggatagcaa
      901 tatgtgacca caatactgta gcgggaagtt accatgcaag aaagttcgtt acaaaattga
      961 acctcccttt gcttgtcctt cccggagtgg aagtctcaac tactcaaggc catctcatcg
     1021 tacttggggt aaaggataac attccatcag gcatgacgcc ccaggaaaca atcaagatag
     1081 caaggcaaaa aggcggagtt gttatagccc ctcatccttt caaaataaga agtatcggca
     1141 atgtggatgg gctggatatt gatgctatag aaacattcaa ttccaggtgc attttcgggg
     1201 aaaatgaaaa agcccgaaaa atggcaatag aacttggaaa accgcaagtc ggaggaagtg
     1261 attcccacat gctgggaacg atcgggttag gatatacgga aatagatgcc caaccttatg
     1321 aagcatcggt attaaatgcc ataagaaagg gaaatacctg cccggggggg aagaacgctc
     1381 cgatttatat tataatactc caggtatttc gcggagtcct cagacgtctg aggacatcac
     1441 ggtcaaaatg atatgagcat cgtttttgaa aagatataaa tgttattcat ccataatatg
     1501 aaacgcgcaa tggaaaaatt tgatgtaata atcgtaggag gaggaataag cgggctatta
     1561 tcagctctta ctctttctaa gcacgggaag aaagtgcttt tgcttgaaaa ggatgatgct
     1621 gtaggaggca attgcaacag ttacatggtg gacggtttcc aggtggatac gggcccccat
     1681 gcgatcaccc acctgcgtga aggacctctg cgcaggttga tggatgagta tttcacatat
     1741 atgccttttt tcgttgatta cgggcactat tatgtgcgta ccgaaaaagg cctcacgaag
     1801 atcccatcca atataaaaga ttttgttact tttgatgttc ttccaagaaa agaccggctc
     1861 atgctttcac aggcattgac gaaagctctg acccagatga tctttggaat gctggatggc
     1921 gatcagcctg tttacgatta tcttcctaaa ggcctttccg gggacacata tgattttgtc
     1981 aatgccatcg catatttcct ttccgggaaa tccatgaaag acacatctgt aaaccgtgta
     2041 ttatacggga gcagttttgt aagggacagc gtatcagtgg atatttttga ggacgttgag
     2101 gtggaatccg gtacttctta tacttcactc ataacagata aattatcaaa ataccagtta
     2161 aaaggtcatc ttacctcatt gagccggctt gccaccaata aagtagccta tgcgcaggct
     2221 taccctcgcg gaggattgag atcactgcta aatgcggtat tatattcaat gccggaaaca
     2281 gtgcaaataa aaaccggttc aagagtgagc aggatattga tggacggtga aaaagcgatt
     2341 ggtgttgcta cagatagtga ttcttattct tcggacatag tcgtttacac aggttttgta
     2401 aaaagcctcc cctgccttgt tccattacca tcggattatg taaagaaact ttcaacgata
     2461 gaccagacac taagccttac tatatggctg ggattgaagg gaaaaatgaa agaatttgat
     2521 tataccggat ccgaggtatg gttcaggggt ggcgcatact gggcgatgcc catcagcaac
     2581 tatgatgcaa gcattgcgcc caaaggtaag cagcttgtgg gattcacttt tgttattgat
     2641 gaaaaaaatg acatcgatag cgaaaagaga aaagcgatgg gattgatcat aaaggctgtg
     2701 cctggtattg aaaaaatggt tgaaatgaca cattaccagg tgacaatacc cgaaaaagcc
     2761 gccgtgacca taaatggata tttcgcagga ccacgatcac ctatcaggaa tctctatctg
     2821 gcagggactg ataccgatag ccgcagcatg ggagtaacaa gggcagcata ttcggttctt
     2881 gagctcttaa aagccatgaa agaagataag atactctgac atttttttgg tgttcacttt
     2941 ttcctgtttt tcatataatc cgaaaaacct gaattaagaa caaaaacaat accgatagtg
     3001 aaaattagaa aacccagcaa ttttgtattt gataagtttt cctttaaaag gagcaatact
     3061 ccggtatgca gaaccaggag gccaaggata attgaggtga tttctttgaa cctgggaaaa
     3121 aaggtgaaat atatcaggcc ggacaaatat gtacccacgc caagataaac aaatgcgata
     3181 tttccaaaat atatccctga aaataaaaat atagacccca gtactgctaa cagggcaatt
     3241 cttttattca ttaatggtat tgatatcagt gaacatgtat taatcttcgt ttttcacttc
     3301 gacttttata tccctgacca atttgatacc cagtcctccg atcctcccgc ctgccgaggc
     3361 aacaaaggtc ataagcataa accatagtcc catgttagtc atcgtgcttg aatcctttcc
     3421 ggacataagc tcagtctcca tgggcggggt tcccggtcct gttggcagtg ataacttgac
     3481 gctgttcatt tgaataacgg aaggtgcagc ttctgctcct gtatatacac tgtacatcga
     3541 atataccgaa aagagtatga atagtagacc aaatgtgagc agaccgtatc ctattccttt
     3601 gatattttta atttccattc tttcacttcg acatttgttt ttagaactat tatatttatt
     3661 aaaattaact atatataaaa atatcctgag tgaacatgat ggaattatat atacttgacc
     3721 tggtcagtta gaagactttt tattttcatc ttgtacgaag aaaccgcaga gcgcgcagag
     3781 gacagagatt acaactcggt gatctctgca tcctctatgg ttgtggaaat agacgataac
     3841 tatttagtgc cccgttactt aaataagaat gaacaaaaat tgagagtgtg agagcattaa
     3901 ccttaaagat tttctttcct tgaaaaacac tgacccgaaa ttatcagaat tgatactttt
     3961 ctttgccgaa aatggacaag aaataaagaa cggattcctg aaaagccaga acaaggccgg
     4021 aacaagtaac atttatggcg aagagcagat ggctcttgat aaatgggcag acgaagtcct
     4081 tataaatgga ttgaagaaaa caaggctagt acgctatatt gcaacagaag agcagccagg
     4141 gatcattgag atcgataatc ccaggaacca gttcggggtt gttatcgacc ctcttgatgg
     4201 ttcttcactc atcgatgtta accttgccgt ggggacaatt atcgggatat atcctggaac
     4261 tgttctcgat aagggtgaga acatgatcgc tgctatgtat attctctacg ggccgcttac
     4321 aacgctaact ttgacaacag gaaatggcgt ccatgatttc gtaatgggtg aaaaaggtga
     4381 attctacctg acagcagaaa atatcaagat ccctgatggg aaaatatacg cgcctggagc
     4441 ccttcgcaag gattacctgc cggcgcattc aaagttcatt gaaagtcttg agaatgaagg
     4501 ttataaattg cgtttcagcg gctcttttgt tgccgatatg caccagatac tccataaggg
     4561 tggggtattc acatatcctg gctttaaagg aaaagaaaaa ggaaagttgc ggctgctttt
     4621 tgaagcaaat cccatgggca ggataatagc agaagcaggc ggggcaatta gcaatggaaa
     4681 aaaagatata ctttctatcc tgccaggtgc tattgataat attactcctg tctatgcagg
     4741 aagcaaaaag gagatcggtt tgatagaaag atacacgcaa gaactatctg tttagaacgt
     4801 gagataaagg ggctggagaa gcccgccctg cttgtaggat tttacgaaac tttgatttta
     4861 cgccgtcttt cctgcaatag ttcaatgaaa tcgtctttat cattgaattt ttcaagctct
     4921 ttattatcaa taattaccgt tccctcgata ttctttgttt tacccgctcc ctggataatt
     4981 aacagggact gggtttgagc gacctctgat atactactca taagatgagc ttttttcacc
     5041 atggtccgtg aatattgcgc aactccggtc aatatcgttg catcttcatc tttgcggtta
     5101 aaagactgtg atacagcgtc aaatggcgcc tgtgaaatgg gcatgacatt aaaccccata
     5161 tcttgcagta tagagaaaac atttgaatgt tctggcaccg tctcggtttt ctgggcatca
     5221 tcccggtgta gtttttcaaa taaattgaca gaaaccgtaa atccccggtc cattacctct
     5281 tcaagcttca ctgcaacatc aacgcttgcc gccatttccc cttcttcata cttgcttatg
     5341 gttcttcgcg aaacaccgag cattgaggcc agagcgccca gggaaatatt ttttttgatg
     5401 cgttcttctt taaggatatt tccctctata tttacataca aaccgccgtg ttcggcataa
     5461 atcaagggtg gtaaatcctc cacgaaataa tcctgcattg ttcttatatc aaatgccgga
     5521 acaccatacc tgaaatatac tacgcctgtt tccaatgaat ggtcacgtgt tttttcacca
     5581 atgactatgg gatatccatg taattgtttt gaaagaaact gcatttccct tgtagtttct
     5641 tcattcaggc cgtcgatgtt tgagatgacc tttatcaaga gcaataaatt atctcttctt
     5701 accgctatat caaaacttct cgggcggatg tcgcagcgtt ctgaaataat aaaacccgcc
     5761 ttactcaaga tgacaaatgc ctgatttatt agcgactctt tattcattaa attttgattg
     5821 tttcttggtc tatataaaag gttgccaata atttttatac ctgtatgtaa ttgagagcct
     5881 catgattatt ggactcgacg ataccgattc aagagatagt atgtgcacga cctatctttt
     5941 agcggtgctg atcgataaac tcaatgctta cggctgtgtg aagga
//
LOCUS       DATGBN010000412         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_72303, whole genome shotgun sequence.
ACCESSION   DATGBN010000412 DATGBN010000000
VERSION     DATGBN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72303"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VKL21_10350"
     CDS             complement(<1..343)
                     /locus_tag="VKL21_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46211.1"
                     /translation="MNLNTLNQKQKFFLFLLISVVSVLKLDILKTISGISPEYLTING
                     AYPWAILFLCGFFLFAKRKELSPVSNSLNFSISGMIIYLLSFFLPANAPEFEIFRLLL
                     ALTGLAFIFFGE"
     gene            complement(376..801)
                     /locus_tag="VKL21_10355"
     CDS             complement(376..801)
                     /locus_tag="VKL21_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46212.1"
                     /translation="MTNKLKHIYIFFLLIIVSLLVFIVHNISQQGTTNTKEIEFNRVD
                     DYNEQLGNITKISVTVYNNDTINHNYTVSSFVDSIIFSSDTVEVSADMPYTYSVMIPI
                     EKKYNADNILIDDPIHSINLTVYRDDSERPLDQIEFKYN"
     gene            complement(798..1970)
                     /locus_tag="VKL21_10360"
     CDS             complement(798..1970)
                     /locus_tag="VKL21_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46213.1"
                     /translation="MKLISIITIAMLALILMAGVGSAASGDGTMIVSPTSVTAGSTGN
                     NFIFTFNNQGYAFTSGSKATIDIPSSWTLPQKTKSREKGYVSVTANGATNVDYSIKGR
                     TITVNFRTTNQNNKGFKINYINVDAPKTSGNNIFTTKTKNDGGLLINIITTQPTVNVK
                     PNHADKLAFASQPSDTVLDGIISPPVKVQIQDHYNNLVIDSNTWVTVAIYSNPGHGTL
                     SGTKSRKAINGVATFDDLSINKAGTGYTLKASSSGLSSVTSSKFDIKVNPPTYTHTIT
                     PSPMVGVRVKDTVTSTSTSGTDMEFKWIRPDGHTASTDSVPRSHDDTYVDSYKPNVAG
                     LWTINVKEFDRHHVKLGESSTSFTVTENSEFGKLGAVLPLFTIGFIYMNYRRKFNK"
BASE COUNT          609 a          370 c          410 g          682 t
ORIGIN      
        1 cctcgccgaa gaagataaag gcaagaccgg tcagggccag aagcaggcgg aatatttcaa
       61 attccggtgc atttgcaggc agaaagaatg agagtagata gatgatcata ccgcttattg
      121 aaaaattgag agaattactc actggtgaaa gttcctttcg ctttgcgaat agaaaaaaac
      181 cacaaaggaa caggatcgcc catgggtatg caccattaat tgtaaggtat tcaggtgaaa
      241 ttcctgaaat tgtttttagt atgtcaagct tcagcacaga tacaacagaa atcaggagaa
      301 acaggaagaa cttctgcttt tgattaagag tattaagatt cattaactat cctataataa
      361 acccattttt cgcacttaat tgtatttgaa ctctatctgg tctaatggtc tttcggaatc
      421 atcacgatag acagtgagat tgatgctatg gattgggtca tcaataagta tattatcagc
      481 attatatttt ttttctatcg ggatcataac tgagtaagta tatggcatat ctgcagaaac
      541 ctctacagta tcgctggaaa aaataatgga atctacaaaa gaggaaacgg tatagttatg
      601 gttgatcgta tcgttattat ataccgtgac agatatttta gttatatttc ccagttgttc
      661 attgtagtcg tccactcgat tgaattctat ttctttggta ttcgttgtcc cctgttgcga
      721 aatgttgtgc acgataaaaa caagcaggct cacaatgatg agaaggaaaa aaatataaat
      781 gtgttttaat ttattggtca tttattaaat tttctgcgat aattcatata tataaaacct
      841 attgtaaaca gaggaagcac ggctcccaat ttgccgaact cagaattttc ggtgacagta
      901 aagcttgttg aactttctcc aagttttacg tgatgacgat caaattcttt tacattgatt
      961 gtccatagac ctgcgacatt tggcttataa gaatctacat aggtatcatc gtgtgatctt
     1021 ggtacggagt ctgttgaggc agtatggcca tctggtctta tccatttaaa ttccatatca
     1081 gtccctgaag tcgacgttga agtaacggta tctttcactc taacgccaac catgggagat
     1141 ggagttatgg tatgtgtata tgttgggggg tttactttga tgtcgaattt gctacttgtt
     1201 acacttgaca gtccgctgct cgaagctttt agtgtataac ctgtgccagc tttgttaatc
     1261 gataagtcgt caaaagttgc gactccattg atggcttttc gactctttgt cccgctcaat
     1321 gttccatgac ctggattcga gtaaatagca actgtgaccc aggtattact gtctatcacc
     1381 agattgttgt aatgatcctg gatctgcact tttacgggtg gggatattat accatcaaga
     1441 accgtgtcac tcggttgtga cgcaaatgca agtttatctg catgatttgg cttcacgtta
     1501 acggtaggtt gagtggttat tatatttatt aacaagccac catcgttttt tgtttttgtt
     1561 gtgaaaatat tatttccaga agttttcggt gcatctacat ttatataatt tattttgaaa
     1621 cctttattat tctgattggt agttctaaaa ttaaccgtta tggtacggcc ttttattgaa
     1681 taatcgacat tagtagcccc atttgcagta actgaaacat atcctttttc tctcgatttt
     1741 gttttttgag gcaatgtcca tgaggatggg atatcaatcg ttgcctttga gccagaagtg
     1801 aaagcatatc cttgattatt aaatgtgaat ataaaattat taccggtgct tccggcagtg
     1861 acgctagttg gactcacaat catagttcca tcaccggatg ctgcactacc tacaccagcc
     1921 atgaggatca atgccagcat tgcaattgta ataattgaaa taagcttcat aatcccactc
     1981 ctccaacgat caatgctata gtcaagttct taaataatcg taaattggta ttttgacaaa
     2041 atcctcctta aaagcagaga taagttgctc t
//
LOCUS       DATGBN010000413         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_538310, whole genome shotgun sequence.
ACCESSION   DATGBN010000413 DATGBN010000000
VERSION     DATGBN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538310"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1896)
                     /locus_tag="VKL21_10365"
     CDS             complement(163..1896)
                     /locus_tag="VKL21_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB46214.1"
                     /translation="MEKEESIKLKALSDIASIDISHEFENILQEILKITCETMNAHSA
                     TLMLVDEITDELKMVSSFGLSTDYIEKVYRAANEAGLPLSSSPSGSVFKTGKYFAVPN
                     IYDEPKNRPWYPIAKEFGFSAQIFTPIKNGLKVIGLLNVYMEEVHDFNEEEINFVNIA
                     ASQASSVVQNARMCIRLKNNIQELNDHKEKLEEKIKEMHNALYSSERYLRTIIESSID
                     GIAVVDDQGKIEFGNDSFFDIVGWPRDELSGKFFNKILADDTKELTLKILDEYHNNRA
                     NTGQNTEAKIVTRDSQIRYLLISQVEIFNNWLKKFVFIARDITEKKMMEQKVNDYHKK
                     LEQSYEELIEADRIKTEFISNITHELLTPLTSIRGFVELLDDETMGKINPEQKKSLEI
                     ILRNSNRLIKLIKELLDTLNLDNNKLGLQFRLVSVNSILSKSIQDIHPQANEKQIMII
                     KDIQPLPEIWGDEDRLVQVIMNLLINAIKFTPHNGKITIRAIEQIEQVKISISDTGIG
                     IPADKLMAVFDRFYQVDGSSNRKYGGIGLGLSICKSIIDKHYGSIWAQSYGQGSTFHI
                     ILPKLRNSGRK"
     gene            complement(1958..2578)
                     /locus_tag="VKL21_10370"
     CDS             complement(1958..2578)
                     /locus_tag="VKL21_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMB46215.1"
                     /translation="MNSGKKIKLFGICASPKKGATDYIVQEALKYANEKYDVETAYFS
                     AMGKKMNFCIHCDYCIREKKGCVHKDDMQEVYDKLKWADAIIIGTPVYQGMVSGQAKV
                     IMDRCRALAAQDLHFILNKPGGAFAVGGDRIGGQEPSIQAILNFYIINEAIPVGGGSF
                     GANLGGAFWSKDKGADGAKEDEEGLKSLRKTMKRLMNLALLLKNEK"
     gene            complement(2737..>3260)
                     /locus_tag="VKL21_10375"
     CDS             complement(2737..>3260)
                     /locus_tag="VKL21_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HMB46216.1"
                     /translation="KHDILILVGDHQSATNEGHYELTGVFLDIAEEFKVERIYALGGY
                     GVGQLVETETVLGAANNIKLVKELKGYGVEFKPNEPGGGIVGASGLLLGLGELRGIDA
                     VCLMGVTSGYLVDPKSAQAVLKVLCKILEIDVDMQALEERASEMEKIVAKLREMEQGQ
                     MPASTDEDLRYIG"
BASE COUNT          860 a          734 c          554 g         1112 t
ORIGIN      
        1 cctgtgccat tgaaaaaaat gttgccataa ggccaatgga cattagaccg ataaaaggcc
       61 agaatatcac ctcaaaaatc gtgaagaaat ttcgtttagc tattatccag cttttataaa
      121 aagaagcgac gattctccca aattcgattc ccggatcaaa cattattttc tccctgaatt
      181 tcttaattta ggcagtataa tgtggaatgt acttccctgt ccatagcttt gcgcccatat
      241 cgacccatag tgtttatcta ttatactttt gcatatggaa agcccgagtc ctatgcctcc
      301 gtatttcctg ttcgatgatc catccacctg gtagaacctg tcaaaaacgg ccattaattt
      361 atccgctgga atacctatgc ccgtatctga aatactgatc ttgacctgtt ctatttgttc
      421 tattgccctg atcgtaattt ttccattatg aggtgtaaat tttattgcat ttatgagcaa
      481 attcattatt acctgtacca gtctgtcctc atctccccaa atttccggaa gtggttgaat
      541 atctttaatt atcataattt gtttttcatt agcctgagga tgtatgtcct ggatggattt
      601 tgaaagaatg ctgtttacgg aaacaagcct gaactgcaaa ccaagcttat tattatcaag
      661 atttaaagta tcaagtaatt cttttatgag tttgatcaac ctgtttgagt ttcgaagtat
      721 tatttcaagg ctcttttttt gctccggatt gatcttaccc attgtttcat catcgagcaa
      781 ttccacaaaa cccctgatgg aagtaagagg tgttagtaat tcatgtgtga tatttgaaat
      841 gaattcagtc ttgatcctgt ctgcttcaat taattcttcg tatgattgtt ccaatttttt
      901 atgataatca tttacttttt gttccatcat ctttttttca gtaatatccc tggctataaa
      961 tacaaatttt tttagccaat tattaaatat ttcaacctgg gatattaata aatatcttat
     1021 ctggctatct cttgtaacta tttttgcttc tgtattttgc ccggtatttg cacggttgtt
     1081 atgatattca tccaaaatct taagggtgag ttcctttgta tcatcggcta gtattttatt
     1141 gaaaaatttt cctgataact catctcttgg ccagcctact atatcaaaaa acgaatcatt
     1201 tccaaactct attttaccct ggtcatcaac aactgcaata ccgtcaatcg aggattcaat
     1261 aatggttcta agataccttt cagaggaata tagggcatta tgcatttcct tgatcttttc
     1321 ttctagcttc tccttatgat cattaagttc ctgtatattg ttcttaagcc tgatgcacat
     1381 cctggcgttt tgaaccacag acgaagcctg ggaagctgcg atattcacaa agttgatttc
     1441 ttcttcattg aaatcatgca cttcctccat gtagacattt aacaagccga tgaccttcaa
     1501 tccatttttt ataggagtga atatttgcgc cgaaaaacca aattccttag caataggata
     1561 ccatggtctg ttttttggct catcataaat gttcggaaca gcaaaatatt ttcctgtctt
     1621 gaaaacactg cctgatgggc ttgatgataa tggcaacccc gcttcatttg ctgctctata
     1681 aaccttttcg atataatcag ttgaaaggcc gaatgaagaa accatcttca actcatccgt
     1741 gatttcatca acaagcatca atgtggctga atgagcattc attgtttcgc atgtaatttt
     1801 taggatttcc tgcaggatat tttcaaattc atgtgaaata tctattgatg ctatgtccga
     1861 taatgctttc agttttattg attcttcttt ttccataacc caaagctctg gatattgttt
     1921 ctggatatac tttaatatat ctcaatatta ttttaagcta tttttcattt ttcagaagta
     1981 aagcaaggtt catcaacctt ttcattgtct tacgcaaact tttcaatcct tcctcatctt
     2041 cttttgcgcc atctgctcct ttatcctttg accagaaagc tcctccaagg tttgcaccga
     2101 atgacccgcc cccgacggga attgcttcgt ttatgatata aaagttaagt atcgcctgga
     2161 tagagggttc ctgtccgcct atgcggtcgc ctcccacagc aaaggcgccg ccaggtttgt
     2221 tcaggataaa atgcaggtcc tgggcggcaa gggctctgca cctgtccatt atgacctttg
     2281 cctgtccgct caccattccc tgatataccg gtgttccgat tatgattgca tcagcccatt
     2341 tgagtttatc atatacctcc tgcatatcat ccttatggac gcatcctttt ttctccctta
     2401 tgcagtaatc gcagtggatg cagaagttca tcttcttacc catcgccgaa aaatatgctg
     2461 tttctacgtc atatttttcg tttgcatatt tcagggcttc ctgcactatg taatcagttg
     2521 cgcctttttt cggacttgcg cagatgccaa agagcttgat ttttttcccg ctgttcacat
     2581 attattaatc gccagggata ctcaaatagc tgacggcgca aatcttaaga atcatatgaa
     2641 aacggtgttc ttgaaatcaa aatgccaaag acggaggtta agaagaaaac accgctaaag
     2701 atcgaataat agtggctttg ccatcatttt tttttctcag cctatatatc ttagatcttc
     2761 atccgttgaa gcaggcatct gtccttgctc catttcccgc agttttgcaa cgatcttttc
     2821 catctcagat gcgcgctctt ccagcgcctg catatcaaca tctatttcaa ggatcttaca
     2881 gaggactttg agcacggcct gtgcgctctt gggatcaaca agataacctg atgtaacacc
     2941 cataaggcac acagcatcga taccgcgaag ctccccaaga cccagaagca atcctgaagc
     3001 tccgacaatg ccgccgcctg gctcattggg cttgaattca acgccatatc cttttagttc
     3061 tttaacgagt ttaatgttgt tcgccgcccc caaaacagtt tcagtttcca cgagctggcc
     3121 tactccgtat cctccaaggg cataaattct ctccaccttg aattcttccg cgatatcaag
     3181 gaatacgcct gttaattcat aatgtccttc gttcgtagcg ctctgatggt ccccaacgag
     3241 tattaaaatg tcgtgtttac
//
LOCUS       DATGBN010000414         5429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_499332, whole genome shotgun sequence.
ACCESSION   DATGBN010000414 DATGBN010000000
VERSION     DATGBN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499332"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VKL21_10380"
     CDS             <1..215
                     /locus_tag="VKL21_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMB46217.1"
                     /translation="ILTPFEIRFERVRKRARSDDMNSMDELKKRDDREKGWGLDKAIE
                     KADLTIDNTDTIGIFQNKIEKLLGNL"
     gene            212..634
                     /locus_tag="VKL21_10385"
     CDS             212..634
                     /locus_tag="VKL21_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HMB46218.1"
                     /translation="MNISVKVLAHVNPTEMQDKVEKAISNFFPVDLKLQETEIQALSG
                     DGDLESLRTLHLRLREERILDTARKIFLNGIKDDTTRFRLNKQVACIGKLNFPPVEES
                     LGSIHVEISTENIGDLLKVIDWLAPQTIDGKPVEEIGL"
     gene            634..1620
                     /gene="ccsA"
                     /locus_tag="VKL21_10390"
     CDS             634..1620
                     /gene="ccsA"
                     /locus_tag="VKL21_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMB46219.1"
                     /translation="MGYTEYLQARDFLGMIGQPVITYILIISLFQGIFSILYLMRKDS
                     KYFDYLIKCLYISTGLYFIGFFILAWFHIQIYNTVLIEYPAALRQMIPVENSRIAAPL
                     WIESEKLYFWAMCASAFALTVKKRKDILAFIGIILSIFSLAVYFFSNPFKDPLPIVNS
                     EITRWYAALSSGDMGIFQLAGTLYGRVTYYYNSTYMWTHPPMLFIAYASLIITFAACV
                     YMLFEKDKLYDEIAYRYAKIGYILLTTGMLIGYPWAIEAWKDSAWWWDPKISGSIMMW
                     VLYSAYLHSRIYVSKGKMWRTTAYLGILCFASLVFTYLLTYIVPGIHSVVQP"
     gene            1617..2219
                     /locus_tag="VKL21_10395"
     CDS             1617..2219
                     /locus_tag="VKL21_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46220.1"
                     /translation="MKFKNYDYYLIILTTLILFMIGAVSLYGITYYNYLAVDSGWTKT
                     AQYSQYIDEMNSYLYPFLVLLLISLGLCIPKRLFEQDILLKFSAIILGITVILTIFSN
                     IETGLGFILVLMTVVQSIVLIMTIRKSKSIRFEKEGYIVRMGSSLLHLGLVLIILNFV
                     SLRDSPYHILIFWFGVIFITIGNVFSFYPEKTASLLPDMG"
     gene            complement(2243..3499)
                     /gene="hemA"
                     /locus_tag="VKL21_10400"
     CDS             complement(2243..3499)
                     /gene="hemA"
                     /locus_tag="VKL21_10400"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HMB46221.1"
                     /translation="MTEISSMLITHTKATIVEMESAWHGGIDQLLVHLKSHELVEECA
                     VLKTCNRVEVYVVSPKGSKVLFEFAKHMKVSSRIIDFLDHEESLRHLLRLTSGLESMI
                     VGEDQILGQVKELFQMAKKAGAVGKTLDTAFNKAIQVGKRTRNETGINKGSVSIGSAA
                     VELAEKELGGLEGKTVLVIGAGEMGTLVAKALANKDIKVIYVANRTFDKAQTLACELN
                     GEALPYEEMERYIPKSDVVISATKAPHFVLTKEIMSRIMEKRKRDIMLIDIGNPRNIE
                     SCAGDLPGVCLHNIDSLRSISEANLDKRRAEAKKAEIIIEEELALLKRQYKRQMADNI
                     ISALYSKVEKIREHEREAAINKLRSRHTIGDIEREVMDDMTNSFAKQILAEPTKILRN
                     AAEHDDERFLDTVAELFKLNGKKEKK"
     gene            complement(3521..4156)
                     /locus_tag="VKL21_10405"
     CDS             complement(3521..4156)
                     /locus_tag="VKL21_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HMB46222.1"
                     /translation="MSGFLPLMLDLNEKEIVIFGGGEVGERKASLFCEHAKVTVVSKE
                     FTPGLNRFHEAGKIKLIKVKDLGEKDISSFLKNAFIVIPAANDVKLNEKIAGIAKESE
                     IFVNRVDDIGDIIIPSVIRRGDIVIGISTGGQSPALSKYIRQKTEEVITPEFSQMSRL
                     QNEIREILKSSVDNQKKRKEILWNIINDSDIWEAFQESYEKAYMKASKHIR"
     gene            complement(4153..4611)
                     /gene="ahbB"
                     /locus_tag="VKL21_10410"
     CDS             complement(4153..4611)
                     /gene="ahbB"
                     /locus_tag="VKL21_10410"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit beta"
                     /protein_id="HMB46223.1"
                     /translation="MDKIDLKLLKTIQDGIPIVSEPFKHIAVAVGLTETEVLERLHDL
                     GKEGVIRRFGASIGHRAIGITANAMCTWNVPDEKVEEVGAIMAGFPEVTHCYERPRYP
                     DWKYNLFTMVHAYSHQECEKIAKEISIATGIKDYSILFSEREFKKTGVRL"
     gene            5156..>5429
                     /locus_tag="VKL21_10415"
     CDS             5156..>5429
                     /locus_tag="VKL21_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMB46224.1"
                     /translation="MKKEPIIPELRDPAFREQVLKTPVGEKIPTCMQCGICAGSCPVT
                     HEMDYSPRELVRMVQFGLKKEVLSSNTIWICTTCFSCSVRCPRGIHP"
BASE COUNT         1463 a         1134 c         1028 g         1804 t
ORIGIN      
        1 ctatacttac accctttgag atccgatttg aaagggtgag aaagcgcgcc aggtctgatg
       61 atatgaatag catggatgaa ttaaaaaagc gagatgacag ggaaaaggga tgggggctgg
      121 ataaggctat agaaaaagcg gatttaacta tcgacaacac cgatactatc gggattttcc
      181 aaaataaaat tgaaaaactg ctgggaaatt tatgaatata tcagttaaag tgctggctca
      241 tgtcaatcca acggaaatgc aggataaagt tgaaaaagcc atatcgaatt tctttccggt
      301 tgatctcaaa cttcaggaaa ccgaaataca ggcattgtct ggcgatggtg atcttgaaag
      361 cttaaggacg ctgcatttgc gtttgagaga agaaaggatc cttgatacgg cgcgtaagat
      421 ctttttgaat ggaataaaag atgatactac aagattccga ttgaacaaac aggttgcttg
      481 tattggcaaa ttgaattttc cgccagtaga ggaaagcctt ggttctatcc acgtagaaat
      541 atccaccgaa aatataggtg acctccttaa agttatcgac tggcttgctc cacagaccat
      601 agacggcaaa cctgttgagg agatcggcct gtaatgggtt atacggaata tctccaggcc
      661 agggatttcc tgggaatgat cggacaacct gtaataacat atattctgat aatatcatta
      721 ttccagggga ttttttcaat tctttatcta atgagaaaag atagtaaata ttttgactat
      781 ctgataaaat gcttatatat ttcaacaggg ctctatttca ttggattctt catcctggca
      841 tggttccaca tccagatata caatacggtt ttgatcgagt atcccgctgc attaaggcag
      901 atgataccgg ttgaaaacag tcgcatagct gctcctttgt ggatagagtc ggaaaaattg
      961 tacttctggg cgatgtgcgc gtcagcattt gctttgacag tgaagaaaag gaaagatatt
     1021 ttagctttta tagggatcat attatcaatt ttttccctgg ccgtatattt tttctcaaat
     1081 ccttttaaag accctcttcc catagttaat agcgaaataa cgcgctggta tgcagcgctt
     1141 tcatcaggag atatgggcat ttttcagtta gcagggacac tttatggcag ggtgacctat
     1201 tattataatt caacgtatat gtggacgcat ccacccatgc tctttatagc gtatgcttca
     1261 ctcatcatca cttttgccgc atgtgtttat atgctttttg aaaaagataa gctctatgat
     1321 gaaattgctt accgttacgc aaaaataggt tacattcttc tcactaccgg tatgcttata
     1381 ggatacccct gggccatcga agcatggaag gacagcgcat ggtggtggga ccccaagatc
     1441 agtggttcga taatgatgtg ggtgctttat agcgcatacc tgcactcccg tatttatgtt
     1501 agcaaaggca agatgtggcg aaccaccgca tatcttggga tactctgttt tgcatcattg
     1561 gtattcacat atcttcttac ctatattgtc ccgggaatcc attcggtggt gcagccttga
     1621 aattcaagaa ttacgattat tatttgatta tccttaccac gctcatcctt tttatgatcg
     1681 gcgcagtaag cctttacgga attacttatt acaactatct tgcagtggat tcagggtgga
     1741 caaagaccgc acaatatagc cagtacattg atgaaatgaa ttcatacctt tacccgttcc
     1801 ttgtattgct tcttatttca ctgggtctat gtattcccaa aaggctcttt gaacaggata
     1861 ttctgttaaa attcagcgcg atcatccttg gaattacagt aatactcaca attttctcga
     1921 atattgaaac gggattgggt tttattctcg tattgatgac agttgtacag tccattgttc
     1981 ttataatgac gataagaaag agcaaatcta tcaggtttga aaaggaagga tacattgtga
     2041 ggatgggctc atcgctcctt catcttgggc ttgttttgat catattgaat tttgtgagtc
     2101 tcagggatag tccctatcac atcttgattt tctggtttgg ggtgatattt attacaattg
     2161 gaaatgtttt ctcattttat cctgaaaaaa ccgcttcgtt gttacctgat atggggtaga
     2221 gaagaccacc tgacctggcg gttcacttct tttccttttt cccatttagt ttaaataact
     2281 ctgcaactgt atccaaaaat ctctcatcat catgctccgc agcattcctg aggatctttg
     2341 tgggttcggc gaggatctgt ttagcaaaag aatttgtcat gtcatccatg acttcacgct
     2401 ctatatcccc gatcgtgtgt cttgatctta atttattgat cgcggcttct cgctcatgtt
     2461 cccggatttt ttctactttc gaataaagag ctgaaataat attgtccgcc atctggcgct
     2521 tgtactgccg tttaagaagc gccaattctt cttctataat gatctccgct ttttttgcct
     2581 cagcccttct tttatccagg tttgcctcgc tgatgcttct taggctgtcg atattgtgaa
     2641 ggcacacacc cggtagatcc cctgcacagc tttcaatatt cctgggattt ccaatgtcaa
     2701 ttagcatgat atcccttttt cgtttttcca ttattcttga cataatttct tttgtcagga
     2761 caaaatgagg ggcctttgta gcacttatca caacatccga tttcgggatg tatctttcca
     2821 tttcctcata tggcaacgct tcaccattta attcgcatgc aagagtctgc gctttatcaa
     2881 atgtcctgtt tgccacatag ataaccttaa tatccttatt cgcaagcgct tttgccacaa
     2941 gcgtccccat ctctcccgcc ccgattacca gaactgtctt tccttcaagc ccgcctaatt
     3001 ccttctctgc caattccact gctgctgaac caatagatac agaaccttta tttatcccgg
     3061 tctcatttct cgtacgcttt cctacctgga ttgccttatt gaatgcggta tcaagggttt
     3121 taccaacagc tccggctttt tttgccatct ggaataattc ttttacctgg ccaaggatct
     3181 ggtcctcacc cactatcatt gactcaagac cggaagtaag gcgcaaaaga tgcctcagtg
     3241 attcctcatg gtcaagaaaa tcaattatcc gggaagagac tttcatgtgt tttgcaaatt
     3301 caaaaagtac tttgcttcct tttggggaga ctacataaac ttctacccgg ttgcaggtct
     3361 taaggactgc gcattcctct acaagttcat gtgacttaag atgcaccaga agctggtcta
     3421 tgccgccatg ccatgcgctt tccatttcca caatggtcgc cttggtatgc gtaattaaca
     3481 tgcttgatat ttcggtcatt gatttcctat ccttttgttt ttaccttata tgctttgatg
     3541 ctttcatata tgctttttca tacgattctt ggaatgcttc ccaaatatca ctatcattga
     3601 ttatattcca gaggatttcc ttgcgttttt tttggttatc cacagaggat tttaatattt
     3661 cgcgaatttc attttgaagc cttgacattt gcgaaaattc aggtgttatc acttcttccg
     3721 ttttttgtcg aatgtatttt gaaagggcag gactttgacc tcctgtggaa ataccgatca
     3781 caatatcgcc tctcctgatt actgacggaa ttattatatc tccgatatca tcaacacgat
     3841 ttacaaatat ctcgctttct tttgcgatcc ctgctatctt ttcattcaat ttcacatcat
     3901 ttgctgcggg aattacaata aaggcatttt tcaaaaaaga ggagatatct ttttcaccca
     3961 gatctttaac ttttataagt tttatctttc ctgcctcatg aaacctgttc aatccgggtg
     4021 taaattcctt gcttacgacc gttacttttg catgctcaca aaatagagag gcttttcgtt
     4081 cacccacctc tccgccgcca aagatcacta tctctttttc attcaggtcc agcataagag
     4141 gtaagaaacc actcataacc tcacacctgt tttcttgaac tcccgttcac tgaaaaggat
     4201 gctataatcc tttataccgg tggcgataga gatctctttt gcaatttttt cacattcctg
     4261 gtgcgagtat gcatggacca tggtaaaaag attatacttc caatcaggat atcgtggtcg
     4321 ctcataacaa tgtgtgactt cagggaaacc tgccataatg gctccaactt cctccacttt
     4381 ctcatcgggg acgttccatg tgcacatggc atttgcggtt attccaattg ccctgtgccc
     4441 tatcgaggcg ccaaaacgtc ttattacccc ttcttttcca aggtcgtgca aacgctccag
     4501 gacctccgtt tcagtaagcc cgactgctac tgcaatatgc ttgaatggtt ctgatacgat
     4561 tggaattccg tcctggatcg tttttaataa tttcaaatct attttgtcca tgttatcagg
     4621 ttatttattt cagaaatatt catatatatg ttcggtgatt atatgaatgg ataggagatg
     4681 aaccaatcaa ttacgttcat acaattatat taatatgata gatgtaaaaa acgttttgga
     4741 agtagtaaat gaagtgctgg ttttgccaat agcgattatt actttctttc tactgattta
     4801 tatagtatta tatctctgga aaaaggatcc tgatgtggtt cggtcaagga ttttcttgaa
     4861 ctataatgaa ataaaaaaag ctttcatatt atttttagca tttgccttta ttttgatctt
     4921 ccatgtatct ttaatataca taccacaatt cttttcatta agttcttctt cttttatata
     4981 tgatttacaa aaaatttttg gattgttact tattttaata atgatagctt ttgtctatgt
     5041 tatctttaga agtataagaa attcaaattc cgaatatact taatattgta tttgaagttt
     5101 gaataaagtt tatatgttat taactcttca tctgatttcc tcttaggggg caaatatgaa
     5161 aaaggaacca ataatcccgg aattgagaga tcctgctttc agggaacagg tcttaaagac
     5221 accagtcgga gaaaaaatcc caacctgcat gcaatgcggg atatgcgcag gctcctgtcc
     5281 ggtcactcac gaaatggact attcgccccg cgaacttgtg cgcatggtgc agtttggcct
     5341 caagaaagaa gtactctcaa gcaacacgat atggatatgc acaacttgct tttcatgctc
     5401 ggtcagatgt cccaggggca ttcatccaa
//
LOCUS       DATGBN010000415         8843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_267296, whole genome shotgun sequence.
ACCESSION   DATGBN010000415 DATGBN010000000
VERSION     DATGBN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267296"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..346
                     /locus_tag="VKL21_10420"
     CDS             56..346
                     /locus_tag="VKL21_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46225.1"
                     /translation="MRVLKPGKHCAILMGDSRRNKHFIPITPRVLMSFLEAGFILRED
                     IIKMQWKMKRTREKWFGKKYDFYLIGHEHLYVFRKPDVEERVTKYKESMKWW"
     gene            406..663
                     /locus_tag="VKL21_10425"
     CDS             406..663
                     /locus_tag="VKL21_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46226.1"
                     /translation="MTVIHIKDDTKELLMKFMAEIRQKEKKRVSYDETIKYLLEGSKR
                     KNKKKLLPLFGCIEKEQAYKDLEELKLLDEKRFERLSRSHS"
     gene            623..1051
                     /locus_tag="VKL21_10430"
     CDS             623..1051
                     /locus_tag="VKL21_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB46227.1"
                     /translation="MKKDLSAYPGVIVDTGVWIEYFLETTLGRKFKEKIVESRISIYI
                     SETVVSEIFYVFCRQKGFQEAKKKIDMVLDGAEVIEGREIQMIAGKYKCERAISLSDC
                     FTMAVSKSTGSPALFKEEEEIRQEINKKAFDVELFFLENI"
     gene            1044..1355
                     /locus_tag="VKL21_10435"
     CDS             1044..1355
                     /locus_tag="VKL21_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMB46228.1"
                     /translation="MSELAPSTFTPETTTVWSFPDRGDWATHVGNYRGNWSPYISRNL
                     ILRYTAPGELVLDQMAGSGTTFVECKLLGWRAVGVDINPDGVVVARNRLDFSYATPDA
                     D"
     gene            1425..1598
                     /locus_tag="VKL21_10440"
     CDS             1425..1598
                     /locus_tag="VKL21_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46229.1"
                     /translation="MLNPASGGASAGVWGRTPAPLKIMLTESVNRTRLSAVSFLKYHP
                     DKKIISGKNIITK"
     gene            1625..1828
                     /locus_tag="VKL21_10445"
     CDS             1625..1828
                     /locus_tag="VKL21_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46230.1"
                     /translation="MVNVTRKELILREIEQVPDDIFEEILDFIRFLRIKSWKESMETA
                     IASESSLRKEWLRPEEDEAWQDL"
     gene            1813..2145
                     /locus_tag="VKL21_10450"
     CDS             1813..2145
                     /locus_tag="VKL21_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMB46231.1"
                     /translation="MARFVKGDVVVLPFPFSDFSLSKRRPALVIAELKGDDVILCLIT
                     SQKVKDENAIPIDENDFEQGSLRKSSHIRPNRIFTVDSNIIFYSAGHLKKKRIDDVIE
                     KVIEILRH"
     gene            2142..2408
                     /locus_tag="VKL21_10455"
     CDS             2142..2408
                     /locus_tag="VKL21_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMB46232.1"
                     /translation="MKSITQDIDHKSLYLIRPLHPRNLILRYTAPKDLVLDQMVGSGS
                     TLVECKLPGRRAVGVDINPDAVMVARNRLDFSYATLDADYVCRR"
     gene            2396..2779
                     /locus_tag="VKL21_10460"
     CDS             2396..2779
                     /locus_tag="VKL21_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMB46233.1"
                     /translation="MQEIKTYVGDAHCLDLIGSETIDLIATHPPYASIIPYFHDCVGD
                     LFSFHNIAEFQDEMRRVAVECMRVLKPGKHCAILMGDSRRNKHFIPITSRVLMSFLEA
                     VFILREDIIKMQWKMKSTRESMKWW"
     gene            2849..3112
                     /locus_tag="VKL21_10465"
     CDS             2849..3112
                     /locus_tag="VKL21_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HMB46234.1"
                     /translation="MVKNIIRISEHALKRIKERDIEIELIEQVIQEPLEVIDVKFGRK
                     AACRKLDDHYLVLIFEKAKDEIVIVTVLKVDNVRLNRYGFSRI"
     gene            3093..3299
                     /locus_tag="VKL21_10470"
     CDS             3093..3299
                     /locus_tag="VKL21_10470"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HMB46235.1"
                     /translation="MVSVEFDSEVNAMYIRFKKGKVDKSEPLADNVIVDVDKNGKAIG
                     IEILLPKEDLRVSNIISKALKVEA"
     gene            complement(3311..4495)
                     /locus_tag="VKL21_10475"
     CDS             complement(3311..4495)
                     /locus_tag="VKL21_10475"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HMB46236.1"
                     /translation="MFRVSMRKSFQFRIYPTKKQEVTLDRTLSTCRHLYNDSLAERKR
                     EAELNRLKQSFDIFPWGKPEWMNYYDQANQLPLSKTVYQEEVYSQVLQNTLKRVDRSF
                     KNFFKGAGYPRFQGRNRYNSFTYPGSGLKLKDGKLTLSKIGNIKIILHREIEGKIKTC
                     TIKKDIDQWYVSFSCKIDIPIIPVEIKTKTGIDVGLTSLITFSNGEQIKPPKHLRASE
                     KRLAREQIHLSKKKLRSKNRNKQRIIAAKVHRKIRNQRKDFAHKTSRKLVDTYDHIVF
                     EDLQIQNMLKNHHLAKSISDASWSQLINLTKSKAEYAGKIVELVNPKGTSQTCICGCS
                     VPKDLSVRIHNCPNCGLVLGRDHISAMLIESRSSTVGTTGIQARLRNLNREPMKRETH
                     LL"
     gene            complement(4703..5842)
                     /locus_tag="VKL21_10480"
     CDS             complement(4703..5842)
                     /locus_tag="VKL21_10480"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HMB46237.1"
                     /translation="MLTKIIIEGTTKVEVPVPDENSNFPPSSVKVFYNPAMRTNRDMA
                     VAAIACFSNDNPDYTYLDALCASGIRGLRIAGEVGLNTTMSDWEEASYGLIKRNIELN
                     NLSNCTVMKRNANVVMLDGSFDIIDLDPFGTPAPFLDAACFSTKRLLCITATDTAPLC
                     GAHKKAGIRNYSAVPLKTEYYPEMGVRILMGAAARTLAKYDKAMTPMLSYTCAHYVRL
                     FVSVKKGIKEADECLKNMGFLSHCFNCGDRKWKLGLAVHMDEKCVVCGHKTSLAGPLW
                     LGKLHEREFCQKLLDEAQKREFKDVLKMIASCRDELDIPMHYDLHKVCKLLGITAMST
                     VDLVSALREKGFQASRTHFSGISFKTDAEMEEIKRVVMEMAGRSC"
     gene            complement(5880..8219)
                     /locus_tag="VKL21_10485"
     CDS             complement(5880..8219)
                     /locus_tag="VKL21_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB46238.1"
                     /translation="MTENEPPLVSGKKGSNKIGVYLCRCGGNIADVVDLQAVEDKLKE
                     DKNVTVNIQDYLCSSMGQDKIKNDIEKGKVDRVVIGSCSPKLHLETFRGMAKNAGLNP
                     GLLEITNIREQASWVHDKEDKTDVTSKVKGLIKGAAARMASIEPLSPLTKTVVDRTLV
                     IGGGIAGITTALELSDSHDVILIEKKPYLGGHMIGLSKTFPTLDCSQCILTPRMVAVH
                     DNPRISMLTQTQVADVQGSPGDYSITLKHSPRHVDIRKCINCGACARKCPTGAIFLPF
                     AQAVPQSYMIDMSKCSNCGACVKICPRDAINLEEKEETSAISVGAVAIATGFEPIDPS
                     FIEEYNYPSPNILTAIEFEEMLSAKSKTGMRLQKADGTFPIRVAFVLCAGSRDFTGKG
                     RKHCSKVCCIYSQKQAQLVKKMKEDSEAWIFYIDMRSSGRRFEEFYHHTQEKGVNYVR
                     GKVAEILPRPDGTLMIQYEDTNLGRKGREIFDMVVLCPALIPSTGTKEIADKLGVSIG
                     DDGFIEEKHVKLDPVNTLNQSVFAAGCVLGPKDIHDSVTEGISAAHKVSQFLGKGMIL
                     IPPEKPMILECNGCSDCVKACPYNALTMEGGKALLSPLACTGCGICVPACPIKGIEIR
                     NFTRDQLKGQLKGILSEAPGVIVFIDPYAYAAADIAGVNRKQYSSLVRLISIPSVHIL
                     DADVVNAAFEYGATGVMLIEGITDEKLTSRSDDLYKKLKKDTRKHKKPIRYSHIETAQ
                     YEKLTDLLNVFASQAGAKAQKMSRKETGEISNKEDDPTS"
     gene            complement(8216..>8843)
                     /locus_tag="VKL21_10490"
     CDS             complement(8216..>8843)
                     /locus_tag="VKL21_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HMB46239.1"
                     /translation="LCYSNLMRARAALQDSALRTIVNEALIEKYDGNAKPKHLLEVIV
                     KDLGLPKLTELVKKPLKIKAVPYYGCLLTRPENDFDSPENPRYLDDLILSLGAEPVKY
                     YYKTKCCGGPILLTDEELALGLTKELLVMAKETGADCMVVTCPMCHLQLDAKQKAVES
                     KFNIRIDMPVIYFTQLIGLAMGFDPKELGLFQHLVPTDKLIAKIGGRQ"
BASE COUNT         2391 a         1879 c         1944 g         2629 t
ORIGIN      
        1 tgcataacat cactgaattc gctgatgaaa tgagaaaagt cgcagtcgag tgtgtatgcg
       61 cgtcctaaag ccgggaaaac actgcgccat cctcatgggt gattcgcggc gcaacaagca
      121 tttcattcca ataacgccgc gggtgctgat gagcttcctt gaggcgggtt ttattctcag
      181 ggaggatatt atcaagatgc agtggaagat gaaaagaacc agggagaagt ggttcgggaa
      241 aaagtatgat ttttacctta ttgggcatga acatctgtat gtgttcagga aaccggatgt
      301 agaggagagg gtgacgaagt acaaggagag tatgaagtgg tggtaggcgg cagtatgaca
      361 tacaattaga aaactttaga taatataaaa tctaaaggga tagatatgac tgttatacat
      421 ataaaagacg atacaaaaga gcttttaatg aaatttatgg cagaaattcg acagaaagag
      481 aaaaaaagag tctcctacga tgagaccatt aaatatttac ttgaagggag taaacgaaaa
      541 aataaaaaaa agttattacc tctttttgga tgcattgaga aagaacaggc ttataaagat
      601 ctggaggagt tgaaattact ggatgaaaaa agatttgagc gcttatccag gagtcatagt
      661 tgatactggc gtctggatag aatatttttt agaaacaact ctcggtcgaa aatttaaaga
      721 aaaaatagtt gaaagcagaa tttcgatata cataagtgaa acggttgttt ctgagatatt
      781 ttatgttttt tgcaggcaga aagggtttca agaagcaaaa aagaagattg atatggtttt
      841 agacggggct gaagttatcg agggtagaga gattcaaatg atcgctggaa aatataagtg
      901 tgaacgtgca atttcacttt cagattgttt cacaatggca gtttcgaaaa gtacaggctc
      961 tccagcgctg tttaaagagg aggaggaaat acgtcaggaa atcaataaaa aagcctttga
     1021 tgttgagctc tttttcttag aaaatatctg aactcgcccc ctccaccttc acccctgaaa
     1081 ccaccaccgt ctggagcttc cccgaccgcg gagactgggc tacgcacgtt ggcaactaca
     1141 ggggcaactg gtcgccctac atctcccgaa acctcattct tcgttatacg gcgcccggtg
     1201 agctggttct tgaccagatg gcgggttcgg gcacaacgtt tgtggaatgc aaactcctgg
     1261 ggtggcgcgc tgtgggcgta gacataaatc cggatggtgt ggtggtggct agaaacaggc
     1321 tggacttttc ttatgcgacc ccggatgctg attaatatag tggatgttat tttattattt
     1381 caagcaagta ttgagtgttc tgatcatatg caaccagtga gttcatgtta aaccccgcca
     1441 gcggcggcgc atccgccggg gtctggggtc gcaccccagc gccgttaaag ataatgttga
     1501 ccgaaagtgt taatcgtaca cgcttatctg cggtttcttt tttgaaatat catcctgata
     1561 agaaaataat atcaggcaaa aatattataa ccaaatgaat tgatatataa ttgggcaaaa
     1621 tatcatggta aacgttacaa gaaaagaatt gatcttacgc gaaatcgagc aggttccgga
     1681 tgatatattt gaggagatac tcgattttat ccgatttctt cgaataaaat catggaaaga
     1741 gagtatggaa actgcaatag caagcgaatc ctcactgaga aaagaatggc ttcgcccgga
     1801 agaagatgaa gcatggcaag atttgtaaaa ggagatgtag ttgtccttcc gtttcctttt
     1861 tcggactttt cactgtctaa gagacgtccg gcacttgtaa ttgcagagct gaagggtgac
     1921 gatgtgatct tatgcttgat aaccagtcaa aaagttaagg atgaaaatgc tatccctata
     1981 gatgagaacg atttcgagca aggcagtctt agaaaaagta gccatataag acctaatcga
     2041 atatttacgg tcgatagcaa tataatcttt tattcagcag gtcaccttaa aaagaaaaga
     2101 atcgatgatg ttattgaaaa agtcattgaa atcctgcggc attgaaatca ataactcagg
     2161 atattgatca caagagtctt tatctaattc gcccccttca tccccgaaac ctcattttgc
     2221 gctacactgc gcccaaggat ttggttcttg accagatggt gggctcgggc tcaacgcttg
     2281 tggaatgcaa gctcccgggg cggcgcgccg tgggcgtgga cataaatccg gacgcggtca
     2341 tggtggcgcg aaacaggctg gacttttctt atgcgaccct ggatgctgac tatgtatgca
     2401 ggagataaaa acttatgtcg gggatgcgca ctgccttgac cttataggga gcgaaacaat
     2461 tgacctcatc gcaacccatc ctccttatgc cagcattatc ccttatttcc atgactgcgt
     2521 tggtgattta tttagctttc ataatattgc cgaattccag gatgaaatgc gccgggttgc
     2581 agtcgaatgc atgcgcgtcc tcaaaccggg aaaacactgc gcaatcctca tgggtgattc
     2641 gcggcggaac aagcatttca ttccaataac gtcgcgagtg ctgatgagct tccttgaggc
     2701 ggtttttatt ctcagggagg atattatcaa gatgcagtgg aagatgaaaa gcaccaggga
     2761 gagtatgaag tggtggtaag catgcccata aatcttggaa atctttaaaa ggtcattcgg
     2821 catacgaggc ttaagtaagt ttttgcagat ggtgaaaaac attattcgga tatcagagca
     2881 tgcattgaag agaataaagg aacgggatat cgagattgaa cttattgagc aagtcataca
     2941 agagcctctt gaagtcatcg atgtcaagtt tggacgaaaa gcagcttgca ggaaacttga
     3001 tgatcattat ttggttctaa tttttgaaaa ggcgaaggat gaaatagtta tagtaacagt
     3061 attgaaagta gataatgtga gattgaatag atatggtttc agtagaattt gactccgaag
     3121 taaatgcaat gtacattcgt tttaaaaagg gtaaagtgga taaatctgag ccgttagcag
     3181 acaatgttat tgtcgatgtt gataaaaatg gaaaagcaat aggcatagag attttacttc
     3241 caaaagagga tttaagggta tcaaatatca tttctaaggc tttgaaggta gaagcgtgaa
     3301 ctactccctg ctaaagcaag tgagtttccc gcttcatcgg ttctctattg agattcctga
     3361 gtcgggcttg aattccggta gttcctaccg tactacttct actttctatt aacatcgcag
     3421 atatatgatc tcttccaagc acaagaccac agttcggaca attatgtatc cttacagata
     3481 ggtcttttgg tactgagcaa ccacagatgc acgtctgcga agttcctttt ggattaacca
     3541 actctactat cttcccagca tattctgctt tggatttagt taaattgatt aattgtgacc
     3601 atgatgcatc tgaaatggac tttgccaaat gatgattttt aagcatgttt tgtatctgca
     3661 aatcttcaaa cactatatgg tcatacgtat cgacaagttt tctgcttgtc ttatgagcaa
     3721 aatcttttct ttgattcctg atttttcgat gaacctttgc agcaatgatt ctttgcttat
     3781 ttctgttctt tgacctgagc tttttcttcg ataagtgtat ctgttctctt gcaagtcttt
     3841 tctcggaagc tcttaaatgc ttagggggtt taatctgttc tccgttgctg aatgttatca
     3901 gggaagttaa accaacatcg attcctgttt tggtctttat ctcaacaggg attatcggaa
     3961 tatcgatttt gcatgagaag gaaacatacc attgatcaat atcttttttg attgtgcatg
     4021 tcttgatttt gccctctatc tctcgatgaa gaatgatctt gatgtttcct atttttgaca
     4081 gtgttagttt tccatctttt agtttaagac cactacctgg atacgtgaat gaattgtatc
     4141 tatttcgccc ttggaatctt ggatatcctg cacccttgaa gaaattcttg aaacttctat
     4201 ccactcgttt tagggtattc tggagtacct gagaatatac ttcttcttga tatactgtct
     4261 ttgacaatgg aagttgattt gcctgatcat agtaattcat ccattcaggt tttccccatg
     4321 gaaatatatc gaaggattgt ttcagtctat taagttctgc ttctcgtttt cgttctgcaa
     4381 gagaatcatt atagagatgt ctgcatgtgg agagggttct atctaatgtt acttcctgtt
     4441 ttttagtggg atagattctg aattgaaatg actttctcat acttactctg aacaagctat
     4501 atatatcttt gatactatat atcagtatgc ctaaccctta aatcttattc tgaacaaata
     4561 ctttttgggg ttaggtatta cactttcaag gggtctgatt catctcatcc ctgaaggaca
     4621 tgagttttct ctaccccttg accccatcgt tataaaagtg aataggtcat cgaatgtata
     4681 atgttgattt gaccaaaaaa gatcagcaag atctacccgc catctccatc acaaccctct
     4741 ttatctcctc catctctgca tccgtcttga aggaaatccc tgaaaaatgc gttcttgatg
     4801 cctggaagcc tttctccctc aaggcagaaa caaggtctac tgtggacatc gcagttatac
     4861 cgaggagctt gcatacctta tggaggtcgt aatgcatcgg gatgtcaagc tcatccctgc
     4921 atgaagctat cattttcagc acatccttga attccctctt ttgggcctca tcaagcagct
     4981 tctggcaaaa ttcccgctca tgaagtttcc ccaaccacag cggccctgca agggatgtct
     5041 tgtgcccgca tacaacacat ttttcgtcca tgtgtacggc aagccccaac ttccatttcc
     5101 tatccccgca attgaaacaa tgagagagaa atcccatgtt cttcaagcat tcatccgctt
     5161 ctttgatgcc ttttttcacc gacacgaaga gccggacata atgagcgcaa gtatatgaaa
     5221 gcataggcgt catggcctta tcatattttg ccagcgtcct tgccgcagcg cccataagga
     5281 tccttactcc catctcaggg tagtattcgg ttttaagagg tacagccgaa tagttcctga
     5341 tacctgcttt cttatgtgcg ccgcaaaggg gcgctgtatc cgtggcagta atacaaagca
     5401 atcgctttgt tgagaaacag gccgcatcaa gaaacggcgc aggggttccg aacgggtcaa
     5461 ggtctattat gtcaaaactg ccatccagca tcactacatt ggcgtttctc ttcattacag
     5521 tgcaatttga gagattatta agttcgatat ttcttttaat caatccatat gatgcttctt
     5581 cccagtcact catcgtggtg ttaagaccaa cttcacctgc aatacgaagc ccccggatac
     5641 ctgatgcgca aagggcatca agatacgtgt aatcaggatt atcatttgaa aaacatgcta
     5701 ttgccgccac tgccatatcc ctgttagttc tcattgcagg attgtagaag accttgaccg
     5761 atgaaggcgg gaaatttgaa ttctcatccg ggacgggtac ttcgaccttt gtggtgcctt
     5821 caataataat ttttgtcagc atgagattgc cacatttggc gggaattcct tatataatgt
     5881 tatgatgtcg gatcgtcctc cttattcgaa atttccccgg tctctttccg gctcattttc
     5941 tgtgcttttg ctcccgcctg cgaagcgaac acgttcagga ggtctgtcaa tttttcatac
     6001 tgcgcggtct caatatgcga ataccggatc ggttttttgt gttttctcgt atctttcttt
     6061 agcttcttat aaagatcatc ggaacgcgat gtaagtttct catctgttat cccttcgatg
     6121 agcataacgc ctgttgcgcc gtactcaaat gccgcattta ccacatcagc atccaggata
     6181 tggaccgatg gaatacttat cagcctgaca agagaagaat actgcttccg gttaactcct
     6241 gcaatatcgg ccgctgcata cgcatacggg tcgatgaata caataacgcc gggagcttcg
     6301 cttaaaattc cttttaactg ccctttcaac tggtctcgtg tgaaatttcg tatctcgatg
     6361 cctttgatgg gacatgccgg gacacagatg ccgcagcctg tgcatgccag gggagataac
     6421 agcgcttttc ctccctccat tgtaagcgca ttatatgggc aggctttcac acaatcactg
     6481 cacccattgc attcaagtat catgggcttc tcaggcggga taagtatcat tccttttcca
     6541 aggaactgtg aaactttatg ggcggcgctt attccttcag ttacagaatc atggatatct
     6601 ttgggaccaa gcacgcatcc tgccgcaaac actgactggt taagcgtatt cacagggtca
     6661 agctttacat gcttttcctc gataaaacca tcatcgccaa tagatacacc aagcttatcc
     6721 gcaatttctt ttgtgcctgt tgagggtatc agagcgggac acagtaccac catgtcaaat
     6781 atttcgcgtc ctttcctgcc aaggttggta tcctcatact gtatcatgag agtgccatcg
     6841 ggtctgggga ggatttcagc gacttttccg cgcacgtaat tgacgccttt ttcctgcgtg
     6901 tgatgataaa attcctcaaa ccttcgtccg gaactgcgca tatcgatgta gaatatccat
     6961 gcttctgaat cttctttcat ctttttcaca agctgtgcct gcttctggga gtaaatgcag
     7021 cagaccttag agcagtgttt tcttcctttt cctgtaaaat ccctgcttcc cgcacaaagc
     7081 acgaatgcga cccgaatggg aaatgtcccg tctgctttct gtaatctcat cccagtcttg
     7141 ctctttgctg atagcatttc ctcaaattca atagcagtga ggatgtttgg ggatggataa
     7201 ttgtattctt cgataaatga agggtcaatt ggttcaaatc cggtagcgat tgcaacggct
     7261 ccaactgaaa tcgcacttgt ttcttctttt tcttcaaggt tgatcgcatc gcgcgggcat
     7321 atttttacgc atgcgccaca attactgcat tttgacatgt cgatcatgta ggactgtggc
     7381 actgcctgcg caaatggaag aaatattgcc ccggtcgggc atttccttgc gcatgcgccg
     7441 cagtttatac atttcctgat atcaacatgg cgtgggctgt gtttcagtgt aattgaataa
     7501 tccccgggac ttccctgaac atctgcaacc tgggtctgtg ttaacatgga gatcctggga
     7561 ttatcgtgaa cagcaaccat cctgggtgta agaatgcact gggagcagtc aagcgtgggg
     7621 aatgttttgg aaagacctat catgtgaccg ccaagatatg gctttttttc tatcaatata
     7681 acatcatggg aatcagaaag ctcaagcgct gtggttatcc cggctatgcc gccgcctatg
     7741 acaagcgtcc tgtccacaac tgtctttgta agaggcgaga gcggctctat tgaagccatg
     7801 cgtgcggcag ctcctttgat caggcccttt acttttgagg tcacatccgt cttatcctct
     7861 ttgtcatgta cccagcttgc ctgctcgcgt atattggtta tctcaaggag gccagggtta
     7921 agccctgcat ttttcgccat gcccctgaaa gtttcaagat gcagcttggg cgagcatgaa
     7981 cctatgacaa cccgatcaac tttccccttc tcgatatcat tttttatctt atcctgcccc
     8041 atgctgctgc acaggtaatc ctggatattt acagtaacgt tcttatcttc tttgagttta
     8101 tcctcaactg cctggaggtc gaccacatct gcaatattgc ctccacatct acaaagatac
     8161 acaccaattt tgttcgatcc tttttttccc gatacaagag gcggttcatt ttctgtcatt
     8221 gcctgcctcc gattttagcg atcaatttat cggtgggaac aagatgctga aaaagcccca
     8281 attccttagg gtcaaaaccc atggcaagac ctatgagctg ggtaaaataa atgacaggca
     8341 tatcgatcct tatattgaat ttggactcga ctgctttttg ttttgcatcc agctgcagat
     8401 gacacatcgg gcatgtaaca accatacaat ccgcacctgt ttctttagcc ataacaagta
     8461 gttcctttgt cagaccaagg gcaagttcct catccgtaag tagtatggga cctccgcagc
     8521 attttgtttt ataataatat tttacaggct cggccccaag gctcaaaata agatcatcga
     8581 gatatcttgg gttctcgggg ctgtcaaaat cgttttcagg gcgtgtcagg aggcagccat
     8641 aataaggaac agctttgatc ttgagaggtt ttttcaccaa ctcggtgagt tttggcaatc
     8701 ccagatcttt cacgatcact tcaaggagat gttttggttt tgcattacca tcatattttt
     8761 caatgagggc ctcattgaca attgttctaa gggcgctgtc ttggagcgcg gcgcgcgcac
     8821 gcatgaggtt tgaatagcac agg
//
LOCUS       DATGBN010000416         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_305521, whole genome shotgun sequence.
ACCESSION   DATGBN010000416 DATGBN010000000
VERSION     DATGBN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305521"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VKL21_10495"
     CDS             <1..773
                     /locus_tag="VKL21_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46240.1"
                     /translation="EFYEEWVKTYQNSFGKFYSIPAPETNKEILENLLSNAEESNKIY
                     RSWIEELEENSRKTQEVLRGEPDTVKYKEVYDLWIKSYGKIFDQLLTLPFRENIKEIF
                     EKITGTPAAEMSKGGASPEAYKEFYALWLNAYQETYGKLFDIQSARPSNEIFESFAQS
                     TSTYLNLYKSWISTLEKLSWKAKELSEQTAEPETYKQYYNLWAKTYEKSFDIFFEITP
                     TVGPFKDILEPARNAAKIYADTFTGGWTKSYPGSAISI"
     gene            complement(1266..1415)
                     /locus_tag="VKL21_10500"
     CDS             complement(1266..1415)
                     /locus_tag="VKL21_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46241.1"
                     /translation="MIATRQYKCPNCKHSISIAMADFIRGIAMTSCGDCGRNAFAIDE
                     VLLNG"
     gene            complement(1701..1874)
                     /locus_tag="VKL21_10505"
     CDS             complement(1701..1874)
                     /locus_tag="VKL21_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46242.1"
                     /translation="MSPSKQNRNYCDNNCLISNEGNPSSHKNSSLCLKQIKDILEERE
                     YFQDARQCLYQIK"
     gene            2083..>2281
                     /locus_tag="VKL21_10510"
     CDS             2083..>2281
                     /locus_tag="VKL21_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46243.1"
                     /translation="MGRIRILKRINFKTFVAIFEELHGEIKKKLAGNPGKVHIVCYIS
                     KSLYDEMSVNAKEFLGFCQSCM"
BASE COUNT          732 a          447 c          444 g          658 t
ORIGIN      
        1 cagaatttta tgaagaatgg gtgaaaacat accagaacag ctttggtaaa ttttattcaa
       61 ttccggcgcc agaaaccaat aaagagatat tggagaacct cctgagcaac gcagaggaat
      121 caaataaaat ctacaggtca tggatcgaag aattagaaga aaattccagg aagacacaag
      181 aagtactcag gggcgaaccg gatactgtaa aatataaaga agtctatgat ctgtggataa
      241 agtcttatgg aaaaatattc gaccagcttc ttacgctgcc gttcagggaa aatataaaag
      301 agatatttga aaaaatcact gggacaccgg cagctgagat gtctaaaggt ggcgcaagtc
      361 ccgaagctta caaggaattt tatgcattat ggctgaatgc ctatcaggaa acctatggca
      421 agttgtttga tatccaatct gcaagacctt caaatgaaat atttgaaagt ttcgcccaaa
      481 gcaccagcac ttacctgaat ctttacaagt cctggatatc tacccttgaa aaattgtcat
      541 ggaaggctaa agaactatca gaacaaaccg ctgaaccaga aacctataaa caatattata
      601 atctctgggc aaagacatat gaaaagtcct ttgacatttt ctttgagatt acgcccacag
      661 taggcccatt caaggatatt ctggagcctg caaggaatgc cgcaaagata tacgcagata
      721 catttaccgg aggttggaca aaatcatatc ctggctctgc aatctcgata taatcatgga
      781 aaaatccagc aaatttaagt taaataagtt agaaaataaa ggaggtgaga taaattatac
      841 gttcaacacc cccggaacaa tactattcag cccatacagg ctcttgcgcg cgcgcactgt
      901 tctggctgga agataggttt gcttcgcaat ctctttctga gttaaaaagc cgctcgattc
      961 aaacacttta aaaaaaagtt ttgctgatgg cagtaaatga tctaacttta tcagttcaat
     1021 ttgtgggcca ttgttggcaa agacgattcc ttctaaacag gaaaaaactg cctcatattt
     1081 tcaatctcgt atatcgaaat cccctgcgct cactttgccc ccctcaggaa gcgcctcagg
     1141 tcttcatctt cttctggatt ttcattttta tttctttctt cagtattata attcatattc
     1201 ttacctcaaa aaataaagaa aggggcgcaa atggatagga taatttaaag cgccccgttt
     1261 tctgtttacc cattcaacag gacttcgtct attgcaaagg cattcctgcc gcaatcaccg
     1321 catgatgtca tggcgatccc tctgatgaaa tctgccattg ctatcgatat cgaatgcttg
     1381 cagttcgggc atttgtattg tctggtcgcg atcatataag ccaccagaca ttatcctgca
     1441 tccgggcatt aacctgaaat tcgcccaacg ctttctcctg atcactttcg ggtgaaggat
     1501 tgatagccct ttttgtttta ctgtattcta ttccagggac aaagtaaatc ccttctgttt
     1561 tcttgtagtg tcattttatc accaaaacaa catagttttc atatatattt aaatcgagta
     1621 aatgagccaa atttgataaa ttcattcggt tttcaggaag acaaaggcaa ttattacctg
     1681 aaacaatgtt tctttaaaaa tcattttatc tgatacaggc actgtctggc atcctggaaa
     1741 tattcacgct cttctaaaat atctttaatc tgtttaaggc atagcgaact gttcttgtgg
     1801 gaagacgggt taccttcgtt acttatcagg caattgttgt cgcaataatt ccggttctgt
     1861 ttagatggtg acaaaacctg cctgtatatt cagggatata tatatcatct ttttatatag
     1921 aacgagaggt caaaaatatg ttcaatcaaa gaagggatcg tttttgccat ccataacaaa
     1981 tggaatggtg aagtatcaga aatagaagaa aatacagagg aaatacagga tgtttttgtt
     2041 agaaaatctg ccaattatag gatttgggta gatgaaaccg gggtggggcg tatccgtatc
     2101 ctgaaaagaa ttaactttaa gacctttgtg gctatttttg aggagcttca tggcgagata
     2161 aaaaagaaac tcgccgggaa ccccggaaaa gtccacatag tttgttacat atcgaaatct
     2221 ctctacgatg aaatgtctgt caatgcaaaa gaatttctgg gattctgcca atcctgcatg
     2281 g
//
LOCUS       DATGBN010000417         7431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_577394, whole genome shotgun sequence.
ACCESSION   DATGBN010000417 DATGBN010000000
VERSION     DATGBN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577394"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VKL21_10515"
     CDS             <1..355
                     /locus_tag="VKL21_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB46244.1"
                     /translation="LQIQNMVQNHHLAKSIHDASWSNLINLTKYKAEYAGKVVELVNP
                     KGTSQTCICGCRVPKDLSVRIHNCPSCGLVLGRDHMSAILIKNRSISTVGTTGLQALL
                     RNLNREPMKREVQYL"
     gene            complement(367..582)
                     /locus_tag="VKL21_10520"
     CDS             complement(367..582)
                     /locus_tag="VKL21_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46245.1"
                     /translation="MTDKNCWEFKKCGRELGGAKVKDLGVCPANPSHGRDCWVVAGTF
                     CGGKVQGSDAQKHATCMVCDWYKEVKR"
     gene            728..1264
                     /locus_tag="VKL21_10525"
     CDS             728..1264
                     /locus_tag="VKL21_10525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB46246.1"
                     /translation="MIMQNSILEISCGSHSKLYETKGKEWDMFVGIDIKMSLCRQAKT
                     QGLEIVCCDAFNLPFKPKSFQDVYIQCFTALLEKEALLDAFRKSDYDEFCEFMGNEWD
                     YAWQVVDPFYELLLESRQVSQHIICLPSCNLEMNKYLVNAVKNLPSVTIDVDYSKNWG
                     GKKKACVMFLDIDCTIYV"
     gene            1305..1733
                     /locus_tag="VKL21_10530"
     CDS             1305..1733
                     /locus_tag="VKL21_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HMB46247.1"
                     /translation="MVRVLATGTFDILHPGHLLYLSKAKSLGDELYVIVARDSMIKHK
                     PKPVIPEAQRLAMVKALHMVDKAQLGSEKNIFEPLCRIKPDLIALGKNQFFDERNLES
                     ELKDHGINAKVVRIHSFKECELCSSASIIRKIMERYGKSN"
     gene            complement(1747..2886)
                     /locus_tag="VKL21_10535"
     CDS             complement(1747..2886)
                     /locus_tag="VKL21_10535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMB46248.1"
                     /translation="MTAKRLKNITESATIRISNLANELKNQGKDIISFSLGEPDFNTP
                     GHIVGAAKASLDRGETHYTPSPGIPELRRAITEKLKTDNNIDTKAGNIIVTPGAKQAI
                     FEVMLSVLQEGDEAVIFDPAWVSYDPCVKLAGAKTVWAQTTRDNGFAPAGPGEFITNR
                     TKLIVINSPCNPTGGVYGKSTLKEIADLAVDKNILVLSDEIYEKIIYDKEHISIGSLD
                     NMQDLTITVNGFSKAYAMTGWRLGYVSAPKEVYEQMVKLHSHSVSQATSFVQYAGIAA
                     LQGDQACVKNMVLEFRERRDLLINGLEKLDIKCTRPDGAFYAFADVSNYGSGEAVADI
                     LLNKAFVATTPGCAFGEAGVDFIRISYATSQERIKEALKRMESVL"
     gene            complement(2958..3293)
                     /locus_tag="VKL21_10540"
     CDS             complement(2958..3293)
                     /locus_tag="VKL21_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46249.1"
                     /translation="MNTITLFRLGMERIGHIIELDEYKINEAKALGKSTMISPKFYNK
                     GVYRVLNSNNGSLDSIAINIEKIAAVTYEGLIKELGKDCVDKSLWKDVPEGEPIFFYS
                     LKLENEFVK"
     gene            complement(3334..4248)
                     /locus_tag="VKL21_10545"
     CDS             complement(3334..4248)
                     /locus_tag="VKL21_10545"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HMB46250.1"
                     /translation="MFRVTFLGTGGTLPTPDRNPSSIFVNREGDMMLFDCGEGTQQQM
                     MRAKTGMKLSAIYITHFHADHFLGIPGLIQTMSFNGREEPFDIYGPIWTKQFVNLLVQ
                     LGFYKLGFVINAHELKNGDIIDKGDYFIKAVGTDHGIPSLGYVLEEKKRIGKFNREKA
                     IELGVPVGRLFSRLQTGEVITINGKTILPSQVMGQSRPGRKIVYSGDTRPCENLENES
                     AGADLLIHDSTLGEDLKDWALETKHSTSKEAAQVARNAKVKQLILTHISSRYSEKREL
                     LLHDARSVFENVKLAEEMMEIEIQLSDK"
     gene            complement(5231..5371)
                     /locus_tag="VKL21_10550"
     CDS             complement(5231..5371)
                     /locus_tag="VKL21_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46251.1"
                     /translation="MELIEIKCRNCHKGIYIENDHVRENMFCTLGCMNIFEIHPKKMD
                     VN"
     gene            complement(5506..5615)
                     /locus_tag="VKL21_10555"
     tRNA            complement(join(5506..5541,5579..5615))
                     /locus_tag="VKL21_10555"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5580..5582),aa:Tyr,seq:gta)
     gene            complement(5676..6860)
                     /locus_tag="VKL21_10560"
     CDS             complement(5676..6860)
                     /locus_tag="VKL21_10560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMB46252.1"
                     /translation="MTSFQSHMNYDVVVVGAGPAGAISAKYAAQNGAKTLLIEEHASI
                     GSPVQCTGLISTKALKECELGEGNFVLSRIRGVFVYAPNGEEACIRGKDIKAHVIDRK
                     VFDRVLVEQAVHKGVDILMKTRFIGMDNGKISVISNGEKKEIHGNIIIGADGIQSCVG
                     RAAGLPRCEKFLSGIQFEAPYIPKDPEYVEIFTGNEIAPGFFGWAVPFSGMARIGLAR
                     NPGRSALYFLERLLHHPVIASRYRGSRTEHVVGGIPMGPPRKTVQGNVMLVGDAAGQV
                     KPTSGGGIYMGAVCAKLAGEVAAKASRKECSTGEYEKKWRAAIGRELELGMRIHKSIG
                     KLSDENLNEFIAFINKPEIQDLITEHGDMDHPSILLQKIMKTGNKLQLIKFMGVAFKT
                     LF"
BASE COUNT         2162 a         1596 c         1410 g         2263 t
ORIGIN      
        1 cctgcaaatt caaaacatgg ttcaaaacca tcatcttgca aagtccatac acgatgcatc
       61 atggtcgaat ttaatcaatt taacgaaata caaagcagaa tacgctggta aggtagtgga
      121 attggttaat cccaaaggaa catcgcagac ctgcatatgt gggtgtagag taccaaaaga
      181 cctgtcagtg aggatacaca attgtccgtc gtgtgggttg gtacttggta gagaccacat
      241 gtcagcgata ttaatcaaga atagatcaat cagtaccgta ggaactacgg gacttcaagc
      301 cctacttagg aatctcaata gagaaccgat gaaacgggaa gttcagtacc tttaggtcgg
      361 agtcgttcac cttttaactt ctttatacca gtcgcatacc atacaagttg catgtttctg
      421 ggcatcgctt ccctgaacct tgccaccaca gaatgttccc gcgaccaccc agcagtcacg
      481 tccgtgtgaa ggattcgcag gacatacgcc caaatcctta actttggctc cgccaagttc
      541 tcttccgcat ttcttgaatt cccagcaatt tttatctgtc atagtatcac atcattattg
      601 ttcacacatt tatttagttt ggtgtgaaaa aaatgtgaat aagatattgg gtaagggaca
      661 cagaggctaa tgcatatcca ttaattaaaa taaaccttac aaccattaaa catgtagatg
      721 gaataacatg ataatgcaaa attcgatcct ggaaataagt tgtggaagtc attcaaagct
      781 atacgagaca aaaggaaagg aatgggatat gttcgtgggt atagatataa aaatgagcct
      841 gtgccggcag gcaaaaacgc aaggtctgga gatcgtatgc tgcgatgctt tcaacctgcc
      901 cttcaagccc aaatcattcc aggatgtata tatccagtgc ttcactgcgc ttcttgaaaa
      961 agaagccctc ctggatgcat tccggaaaag cgattatgat gagttctgcg agttcatggg
     1021 caatgaatgg gattacgcct ggcaggtggt ggatccattc tatgaactgc tgcttgaatc
     1081 caggcaggtt agccagcaca taatttgcct tccctcgtgc aatcttgaaa tgaataagta
     1141 tcttgtcaac gctgttaaga atctgccatc tgtaacgatt gatgttgatt actcaaagaa
     1201 ctggggtgga aaaaagaaag cctgtgtaat gtttctggat attgactgta cgatatacgt
     1261 ttaattacaa acatttttac caattaagaa taatcatgat gcctatggta agggtccttg
     1321 caaccggtac ttttgatatt ctccatcccg gccacctgct atatctttca aaagcaaagt
     1381 cgctggggga tgagctttat gtgatagtgg cgcgggactc catgataaaa cacaaaccaa
     1441 aacctgtaat acccgaagcg caaagattgg ctatggtaaa agcgctgcat atggttgaca
     1501 aggcgcaact ggggagtgag aaaaatatat ttgaacctct ttgccggata aaacccgatc
     1561 ttatagccct tggaaaaaac cagttcttcg atgaaagaaa tcttgaatct gaattaaaag
     1621 accatggtat aaatgcaaaa gtagttcgga tccactcatt caaagagtgc gaactatgca
     1681 gcagcgcttc gatcattcga aaaatcatgg aacgttatgg gaagtcaaac tgagcggttt
     1741 catttgctaa agtaccgatt ccatccgctt cagggcttcc ttgatgcgct cctgggacgt
     1801 agcataagaa attcttataa aatcaactcc ggcttcaccg aaagcgcaac cgggtgttgt
     1861 ggcgacaaac gctttattaa gcaggatatc tgcgactgct tctcctgacc cgtaattgct
     1921 tacatcggca aaagcataga aagcgccatc cggccttgta catttgatgt ccaatttttc
     1981 caatccattt atgagcaggt ctcttcgttc cctgaattca aggaccatat ttttgacaca
     2041 tgcctggtcg ccctgcagcg ccgcgatacc tgcatactgg acaaaggaag ttgcctggct
     2101 tacggaatgc gaatgaagtt tgaccatttg ctcgtaaacc tcctttggcg cgcttacata
     2161 accgaggcgc cagcctgtca ttgcatatgc ttttgagaaa ccattgacag ttattgtcag
     2221 atcctgcata ttatccaatg atcctatgct gatgtgttct ttatcataaa ttatcttctc
     2281 gtatatttca tccgataata ccaatatatt cttatcaaca gcaaggtcag ctatctcctt
     2341 aagagtggat ttgccataaa cgcccccggt tggattgcag gggctgttta ttactatgag
     2401 ctttgtcctg tttgtgataa attctcccgg tcctgcgggt gcaaaaccat tatcacgggt
     2461 tgtttgcgcc caaacagtct ttgctcctgc aagcttcaca caagggtcat aagataccca
     2521 ggcaggatca aaaattacag cttcatcacc ttcctggagc actgagagca tcacttcaaa
     2581 aatcgcctgt ttggccccgg gtgtaactat gatatttccc gcttttgtat ctatattatt
     2641 atcggtcttt aatttctcag ttattgcccg ccttaattcc ggtattccgg gagaaggtgt
     2701 gtaatgggtc tcgccccgat caagggaagc cttggcagcg cccacaatat gtcccggagt
     2761 gttaaaatca ggttccccca gactgaaact tattatatcc ttgccctggt tctttaattc
     2821 attcgcaaga tttgaaattc gaatcgttgc cgactcggta atgtttttca gtctttttgc
     2881 agtcataaag ttttgcttca aactaattta ttaatattta aggcattcca ataaagaaaa
     2941 aatacagtgc ataatttcta tttgacgaat tcattctcaa gctttagcga atagaaaaat
     3001 atgggttcac cctcaggtac atccttccag aggcttttat ccacacaatc cttgccaagt
     3061 tccttgataa gaccttcata ggtgaccgca gcaatttttt caatgttaat tgcaatgcta
     3121 tcaagagaac cattatttga atttagtacc ctgtaaactc ccttattata gaatttaggg
     3181 gagatcatcg tgcttttccc aagcgctttt gcctcattta ttttatactc atccaattca
     3241 ataatatgcc ctattctttc cattcccagc ctgaataatg ttattgtgtt caaacttatc
     3301 acttatgaaa tatgtacatg cgtgtattta tttctattta tcagaaagct gtatttcgat
     3361 ttccatcatt tcttctgcga gtttgacatt ttcaaaaaca ctcctggcat catgcagcag
     3421 cagctctctt ttttcactat accttgaact gatatgtgta agtatcaatt gcttcacctt
     3481 tgcattcctt gcgacctgtg ccgcttcttt tgaagtagaa tgtttggttt caagcgccca
     3541 atctttcaaa tcttctccaa gtgtgctgtc atgaatcaat agatcagctc ctgcgctttc
     3601 attttccagg ttctcgcacg gtcttgtatc cccggaatac acgattttcc tccccggacg
     3661 actctgcccc ataacttgtg agggcagaat tgtctttcca tttatagtga ttacctcacc
     3721 agtttgcagt cttgagaata ggcggcccac aggaacgcca agttctattg ctttttccct
     3781 gttgaacttt cctatgcgtt ttttctcttc aagcacataa ccaaggcttg gaatgccatg
     3841 gtcggttcca actgctttaa tgaaataatc tcctttgtca ataatatcgc cattttttag
     3901 ttcgtgcgca tttatgacaa aacccagctt gtaaaaccca agctgtacga gcagatttac
     3961 gaattgcttt gtccatattg ggccgtatat gtcaaacggt tcttctcttc cgttaaatga
     4021 catggtttgt ataagcccgg gaattccgag gaaatggtca gcatggaaat gagttatata
     4081 aattgcgctt aacttcattc cggtctttgc ccgcatcatc tgctgctgtg tcccctcacc
     4141 gcagtcaaat agcatcatat cgccttccct gtttacaaaa atggaggatg gattacggtc
     4201 tggcgtggga agtgttccgc cagtaccaag aaaagttaca cgaaacatga agtgcataat
     4261 tgtgctgggt atttaaatag gttaagaagc acacaattat cctacaggaa tcattaaaat
     4321 tcgttctatt actttcatca ttatgttcat tttgttaacc gaaaatgtat atatgccatg
     4381 ataattattg taagtattag aaggtaattt ttttggcaca tatcccgcac acacccccgc
     4441 aacccccgct ttatccagcc aaaaaaccag gagaaaatca tgacacatga aaagaaggaa
     4501 ataattaggg aaaaaactga aagaatgaat gaaggaaagg aagagaggga agaaagaagc
     4561 aggaaattct tacctatttt tttctggaca gattgagagt aatgattcat ttaaagtcca
     4621 tcctcaaaaa atggacttta cttagttttt tttcaaagag ccggttcagt cctgataaag
     4681 aacctgtgga caagaaatat aaaaaaaccg aatgttacag cgcccaacaa tgcactttgc
     4741 caatcaatga ttgcattttg cagagaaaat tttaacataa tataaataat aaaataagaa
     4801 attgatgctg taagggttaa agcaatcgtc cttttcataa tacaaaaatg tcatttaaat
     4861 attaagggat tttccaaaat tgaatccaaa ttagaaccat atgttacgga aaagttcata
     4921 tccttacaat cattataagt atgacagcaa atgccaggag gcgaacaaac taatgtccaa
     4981 taagaatata gaaactgaaa aagaggaaga aagaggatgc ggacgattgt gtcctgattg
     5041 tccgatgaaa aatgatcgtg attttatgaa aaagagtgcc ttataaataa taggacaata
     5101 tttgtctact ataattttcc atacaattgt tttaataata aagacttttt ataattatac
     5161 acaaatatat aatattcatg atataaaagt aatattgaac gcaacagttt aaaaaaagaa
     5221 aaaaggatta tcaattgaca tccatttttt tagggtgtat ttcaaatata ttcatgcaac
     5281 ccaatgtaca gaacatattt tccctgacat ggtcattctc tatgtatatt cctttgtggc
     5341 aatttctgca tttgatttct attaattcca tgttgcattc tccgttaagt ttcatgcttg
     5401 aatggtgcta ttatgagcta atatggatat attaattgct gttaaattat taaaataatg
     5461 cttataattg ccattataat aagcattcca aaaataaaaa aaacatcccg cctcccagac
     5521 tcgaaccggg gacttcgcgg tgcctgcgcg ttattatgtg ctttgcacat tatccatact
     5581 acagccgcgc actctagcca ctgagttaag gcgggattgc ccttgaataa gcacaaatat
     5641 acttaaccct tttcgttcct ttccatgaaa tcgcgctaaa atagcgtttt gaaggcaact
     5701 cccatgaatt ttatgagttg cagcttattt cctgttttca ttattttctg aagaaggatt
     5761 gaaggatgat ccatgtcgcc gtgctccgtt atgagatcct ggatttcagg tttgttgatg
     5821 aatgcaatga attcgttcag gttttcatca ctcagttttc ctatgctttt atggatcctc
     5881 attccgagct caagttccct accaatagca gcgcgccatt ttttttcata ttctcccgta
     5941 gagcattctt tccgggatgc ttttgctgcg acttcacctg cgagttttgc acaaaccgcg
     6001 cccatgtaaa ttccccctcc cgatgtaggc ttgacctgtc ctgccgcatc ccccacaagc
     6061 ataacattgc cctgaactgt ctttcgcggc ggccccatgg gaatgccccc cacaacatgt
     6121 tccgtgcgtg aacccctgta tcttgaagca atgacaggat gatgtaaaag tctttcaagg
     6181 aaatataggg cagatcgacc tggattcctg gcaaggccaa ttcgcgccat acctgaaaat
     6241 ggtacagccc agccaaaaaa tccgggagca atttcattcc cggtaaagat ctccacatat
     6301 tcagggtctt ttgggatata aggagcttcg aactggatac ccgacaggaa cttttcacat
     6361 ctgggaagcc ctgcagctct tcctacacaa ctctgtatgc catcggctcc gatgattata
     6421 ttcccgtgaa tttctttttt ttccccgttt gaaattacag atatttttcc attatccatt
     6481 ccgataaaac gggttttcat aagaatatcc acacccttat gaactgcctg ttcaacaaga
     6541 accctgtcaa agactttccg gtctatgaca tgtgctttta tatcttttcc ccggatgcag
     6601 gcttcttcac catttggagc atacacgaag acgcccctta ttcttgaaag aacaaagttg
     6661 ccttcaccca gttcgcattc cttgagcgct tttgtactta tgagacccgt gcactggact
     6721 ggtgagccga tgcttgcgtg ttcctctatc aggagggttt ttgccccgtt ttgcgctgca
     6781 tattttgcac ttattgctcc tgcaggaccg gctccgacca ctacaacgtc atagttcatg
     6841 tgactctgaa aggatgtcag ggttaaaaat ggttttggga aagttcagct tagagtaatt
     6901 aatattccat gtttatcagg tttagtctta atagaacgcg gatgacacgg atcacgcgga
     6961 ttttcacgga tccgtgtgta tcctcccacc aacgccagcc ccaaaaaggg aaaagggtaa
     7021 gggggaaacc ggaaattcat cataactcca ttttttcaag ttcatttcat gacttattta
     7081 ccattattca tacataaata tctcctattt tgatccaatc tgaccattga ccatttactc
     7141 aatttaattt ttgataccaa tatattaagc cacaaagctt gtgacacttg tgcacgagtt
     7201 gacaggacta aatggagaat taaagcaaaa taataatgtt ttaaatttcg atactaatat
     7261 attatgctga aatgtttgtg acgcttgtgt acgtcagatt gtgtaaaaac tattttttaa
     7321 gtctgtttga tttccactcc ccaatcaaac ccttatgaac tgcctttcat gccaaaatca
     7381 tgaaaaatgg tatttttcta tggaattcta tgtcagaacc tcttgatttt t
//
LOCUS       DATGBN010000418         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_499592, whole genome shotgun sequence.
ACCESSION   DATGBN010000418 DATGBN010000000
VERSION     DATGBN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499592"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /gene="cofC"
                     /locus_tag="VKL21_10565"
     CDS             <1..521
                     /gene="cofC"
                     /locus_tag="VKL21_10565"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HMB46253.1"
                     /translation="ILSTSMIEVEGANTVLTEKGLNEALNEYLGKMSSHNINEPVLII
                     MADIPLVSTKNIKDIVSTNADIVIAPGRMGGTNAIFIRHPLSFHVDYYGASFLKHLKI
                     ASGLHTEIFDSFNISTDIDEVSDLTEVLLHGKGQSQEYLTKIGINIQAEGGRVGVKRE
                     TFSASLNRKKSI"
     gene            complement(514..1224)
                     /locus_tag="VKL21_10570"
     CDS             complement(514..1224)
                     /locus_tag="VKL21_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HMB46254.1"
                     /translation="MEISLLIRLIIAHLLSDFVFQSDSWINQRFKDGWYSKSLYLHGI
                     IAGILAYFFSGLWSLIWIPIAVAVTHILIDGIKAKYENNIHSFLTDQLGHFIVLLAIW
                     IWIIKPKPDDITVLEQIFLPGIKIWVIIAAYIIILWPSSKLISKITETWREDIENKDS
                     KDESLEKAGRWIGWLERFLILTFVLLEQYGAIGLLVAAKSIFRFSETRKVGEYVLIGT
                     LLSFAIAILLGLIASLFI"
     gene            complement(1252..>1611)
                     /locus_tag="VKL21_10575"
     CDS             complement(1252..>1611)
                     /locus_tag="VKL21_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46255.1"
                     /translation="VVRTPWAEINEELRTECALLDALIQRWTKEQAEAILYQIQRPKF
                     NVFPSIAQEDLEHSKQGLTQVETAKILNISQSAVFQRLKIAGYWAVQVFLEHFKMIIQ
                     DKVRESNIRLEPYNHIK"
BASE COUNT          492 a          333 c          285 g          501 t
ORIGIN      
        1 atatactatc cacatcaatg atagaagtcg agggcgccaa tacagtgctg acagagaagg
       61 ggttaaatga agccctgaat gaatatctgg gaaaaatgtc ctcgcataat ataaatgaac
      121 ctgtccttat tattatggca gatatccccc tggtctcaac aaagaatatc aaagacattg
      181 tttccacaaa tgccgatatc gtgatcgcac ccggcaggat gggaggaaca aatgcaattt
      241 ttatccgcca tcctttaagt tttcatgtgg attattacgg agcaagtttc ctcaagcatc
      301 ttaaaatcgc atccggcctt cacacagaga tctttgattc atttaatatc agtactgaca
      361 tagatgaagt ttccgacctc acagaagtcc ttctccacgg gaaagggcag tcgcaggaat
      421 acctgacgaa aataggcata aatatccagg cggagggtgg tcgcgtggga gttaaaagag
      481 agaccttttc cgcatctcta aaccgtaaaa agtctatata aacaagctcg caatcaagcc
      541 taacaagatc gcaatagcga aactaagtaa agttccgatc aaaacatact ctcccacttt
      601 ccttgtttca ctgaatctga aaattgattt tgccgccacc aataaaccta tcgctccata
      661 ttgttctaaa agaacaaaag taagaattaa aaagcgttcc aaccaaccaa tccatcttcc
      721 agctttttcc aggctttcat ctttagagtc tttattttcg atatcctctc tccatgtttc
      781 tgttattttg ctgattaatt tactgcttgg ccaaagtatg atgatataag ctgctatgat
      841 aacccatatt tttattcctg gcagaaagat ttgttctaaa acggttatgt catcgggttt
      901 tggttttatt atccatatcc agatagccag aagaactata aaatgcccaa gttgatcagt
      961 taagaaagaa tggatattat tttcgtattt tgcttttatt ccgtcaatta aaatatgtgt
     1021 gacggcgacc gcaataggaa tccagattaa actccatagt ccggagaaaa aatatgcaag
     1081 gattccagca attatcccgt gcaaatagag gcttttagag taccaaccat ccttaaacct
     1141 ctggtttatc catgaatcgc tttgaaatac aaaatcacta agcagatgtg caattattaa
     1201 acggatgagc aatgatattt ccatttgcgt gaccattata agcctcctac cttattttat
     1261 atgattataa ggctctaacc ttatattgga ttctctaacc ttatcttgaa taatcatttt
     1321 aaaatgttct aaaaatacct gaacagccca gtagcctgca atttttaaac gttggaatac
     1381 tgccgattgg cttatattca gaattttagc tgtctctact tgtgttaatc cctgttttga
     1441 gtgttcaaga tcctcttggg ctatgctcgg aaatacgttg aattttggtc tctgaatttg
     1501 atataaaatt gcttcagctt gttcctttgt ccatcgttga attagcgcat caagtagggc
     1561 acattctgtt cgtaattctt catttatctc ggcccagggt gtccttacga c
//
LOCUS       DATGBN010000419         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_112086, whole genome shotgun sequence.
ACCESSION   DATGBN010000419 DATGBN010000000
VERSION     DATGBN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112086"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="VKL21_10580"
     CDS             complement(<1..124)
                     /locus_tag="VKL21_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HMB46256.1"
                     /translation="MKAVLGLEDGTFVIGEGLGVEGIVRGELVFTTQYTGYEEAL"
     gene            240..1199
                     /gene="mch"
                     /locus_tag="VKL21_10585"
     CDS             240..1199
                     /gene="mch"
                     /locus_tag="VKL21_10585"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HMB46257.1"
                     /translation="MISVNETGLCVFNEMLDFANELNIEIHEQKNGSTVVDAGVNVSG
                     GYEAGMYLSRICLADLADLEYTTFGLGGIMVPAIRVSTDHPVVACMASQYAGWRISAG
                     KYFAMGSGPGRALSLNPKKLYAQINYKDSYSDAVLVLESDKLPPEEVTDKIAKDCNVD
                     PGNLHIVVAPTASIAGSVQISARIVETGIHKMESIGFDINTIKSGYGIAPISPIIGDS
                     TKCMGSTNDCIIYCGKTYYNVKYDVDKVKELIVKVPSGTSSSYGKPFFTTFKEAGFDF
                     FKVDAGVFAPAEITINELNSAKTFTSGKIDQEVLMKSFGISRL"
     gene            1254..>2439
                     /locus_tag="VKL21_10590"
     CDS             1254..>2439
                     /locus_tag="VKL21_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMB46258.1"
                     /translation="MVSYMNSYFFLGSIAVYLIGAISALVFNKKNRLCTYASFFSASV
                     ASIMGIIFSLSVLSGNTFNYVIQGSTFLSYGFFVDKLSAFFILIISITAFAVSIYSTG
                     YITVYFGKKNIGYLGFLYNIFILSMVLVVSSNNAIMFLIVWELMSIISYLLVMFEHEK
                     KETRKAGFIYIVMTHIGTGFIILSFLILASSTGSFNFESFRLAGSTMPPHLKDLTFLF
                     ALIGFGTKAGIVPLHIWLPYAHPAAPGNVSALMSGVMIKTAIFMLIRVFFDFLGAGTA
                     WWGILVLIVASISALIGVMYALMEHDLKRLLAYHSVENIGIILIGVGVSMIFMASGHP
                     DLAAFGLIAGLYHTINHAVFKSLLFLGAGSLVYSTHTRNIEEYGGLIKKMPWTTLFFL
                     IGA"
BASE COUNT          656 a          517 c          516 g          750 t
ORIGIN      
        1 tcaaagcttc ctcatagcct gtgtactggg tcgtaaaaac aagttcgccc cggacaattc
       61 cctctacacc aagcccttca cctataacga atgtaccatc ttcaaggccg agtactgctt
      121 tcatgaccgc ttaactgtaa aggcatcgta aatagattac gcaaattaga gtatttgccc
      181 gaacaaaata aacagattta tgtgtccgga atccaacaat taatttcgtg gtaagaaata
      241 tgataagtgt aaatgaaacc ggactctgtg tgtttaacga aatgcttgac tttgcgaacg
      301 aattgaatat tgagatacat gagcaaaaaa acggttccac agttgttgat gcaggcgtga
      361 acgtatccgg gggttatgaa gctggtatgt acctatcgag gatatgcctt gcggacctcg
      421 cggatctcga atatacgact tttggcctgg gtgggatcat ggttcccgcg atcagggtca
      481 gcacggatca tcctgtcgtt gcttgcatgg cttcgcaata tgcgggatgg cgtatcagcg
      541 cgggaaaata ttttgccatg ggttcaggtc ctggccgtgc attatccctt aatccgaaga
      601 agctctatgc ccagatcaac tacaaggatt cctatagtga tgctgttctg gtattagaat
      661 ctgataaact gccgcctgag gaagtcacgg ataaaattgc aaaagattgt aatgtggatc
      721 caggcaatct tcatatcgtt gttgctccta ctgcttctat cgccgggtca gtgcagatat
      781 cagcccggat cgttgagacc ggcatccata agatggaatc tatcggtttt gatatcaaca
      841 ccataaaaag cggttacggc atcgctccta tatctccaat catcggagat agtacaaaat
      901 gtatgggttc cacaaatgac tgcatcattt attgcggaaa gacatactat aacgtgaaat
      961 acgatgtgga caaggtgaaa gaactgatcg tcaaagtgcc ttcgggaacc tcatcctcct
     1021 atggcaaacc cttctttacc accttcaagg aagcaggctt tgacttcttc aaggtggatg
     1081 caggcgtttt tgcccctgcc gagataacaa taaatgaact caacagcgcc aagaccttta
     1141 caagcggtaa gatagatcaa gaggtgctta tgaaatcctt cgggatttca aggctttaat
     1201 ttttcgggtt tcaaattctc tagcaagtct taaatataga atatgagttt atcatggtct
     1261 catacatgaa ctcctatttc tttctgggaa gtattgcagt atacttgatc ggggccatat
     1321 cggccctggt attcaataaa aaaaatcgct tatgtacata tgcatcattt tttagcgcat
     1381 ctgtggcatc gataatggga attattttct cattatctgt tctatcaggc aataccttta
     1441 attatgtcat ccagggttca acgttcctga gttatggctt ttttgtcgat aaattatccg
     1501 cattttttat tcttatcata tcaattacag catttgccgt ttcgatatat tcaacaggat
     1561 atattaccgt atatttcggg aaaaagaata tcggttacct gggattcctg tataatatat
     1621 tcatactttc aatggtcctt gtagttagct ccaataacgc catcatgttc ctgattgtct
     1681 gggaattaat gtctataatt tcctatttgc ttgttatgtt cgagcatgaa aaaaaggaaa
     1741 ccagaaaagc cggttttatc tatattgtga tgacacacat cggcacaggt tttatcattt
     1801 tatcattcct tatacttgca agttccacag gaagttttaa tttcgaatca ttccgcctgg
     1861 ccggttcaac tatgcctcca caccttaagg acctgacttt cctttttgca cttatcggtt
     1921 ttggcacaaa ggcaggcatt gttccccttc atatctggct cccatacgct catcctgcag
     1981 cgccaggtaa tgtatccgcg ctcatgtcgg gagtaatgat caaaactgct attttcatgc
     2041 tcatccgtgt attttttgat ttcctgggcg caggtactgc ctggtggggt attttagtgt
     2101 tgatagtagc ttcaatttcg gcgctaattg gcgtaatgta tgcccttatg gaacatgatc
     2161 taaaacgcct tcttgcttat catagcgttg agaatatagg aattatcctg ataggggttg
     2221 gtgtttcaat gatctttatg gcttcagggc atcctgacct tgcagccttc ggattgatag
     2281 cgggcctata ccataccatc aaccatgcag tatttaaatc gctgttgttc ctgggagccg
     2341 gttccctggt ttattcgacc cacacaagaa atatcgaaga gtatggggga cttatcaaga
     2401 aaatgccctg gacaacgctc ttttttttga taggggcaa
//
LOCUS       DATGBN010000420         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_189453, whole genome shotgun sequence.
ACCESSION   DATGBN010000420 DATGBN010000000
VERSION     DATGBN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189453"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VKL21_10595"
     CDS             <1..411
                     /locus_tag="VKL21_10595"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMB46259.1"
                     /translation="LPGIDMYGIFIEKQQKKSKIDMHNLLSEFLPSRLAKIWCASNIQ
                     SKPVNQYNEKELKKIAHQIHNWEIKPDTTEDFKTAEVTLGGIDTSELSSKTMESRNIK
                     GLYFTGEVLDVTGQLGGYNLHWAWASGFVAGQYA"
     gene            750..2627
                     /gene="cooS"
                     /locus_tag="VKL21_10600"
     CDS             750..2627
                     /gene="cooS"
                     /locus_tag="VKL21_10600"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HMB46260.1"
                     /translation="MIPVSAHKSVIEMHNILHKDNITNSFDRADAQGKRCSFCEQGIS
                     CQLCSNGPCRIKPGAERGVCGIDADAMAMRNMMFLNTMGTAIYTYHAKSVAKTLKATA
                     LGKTPFKITDEAKLRSFAGKLGVKTDGSTKEIALALADAMTAEINSDSDIELSNVLRF
                     APKSRIELWRKLGILPGGPLHEVMDSVSSTMTNVDGDYVSLAKKALRLGISCIYGSQI
                     PLEMGQDILFGTPQPHAVNVDLGIIDPAYVNIAANGHEPFMGAALIKAAHEAKNQETA
                     RKAGAKGLHIIGSIETGQELVQRYNIDDVFVGMTGNWLSIEPALATGGIDVFAMDMNC
                     SPPGMGEYQDKYNTTLISVSKLVNVPGMKDQMAYKPEDAAAQAQKLIDMAIENFKKRK
                     GKVTNPSSRKQNAVIGFSTEACLGALGGTLDPLLDVIKKGTLKGVVALVSCTTLRDGG
                     QDINTLKMARELIKKDILVISAGCGNAALQVGGLTTLEAKEEAGSGLKAVCELLKIPP
                     VLSFGTCTDTGRIALLVTAVADALGVDPSALPVAVTAPEYMEQKATIDAIFALAFGLY
                     THVAPLPPVAGAPRLVKLLTVDLEGITGGKIAVETDMVEAAKGIEAHIMKKRAALGLP
                     N"
     gene            2885..>3776
                     /locus_tag="VKL21_10605"
     CDS             2885..>3776
                     /locus_tag="VKL21_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMB46261.1"
                     /translation="MQAVNRKHGTNFKEYSELYQWSIQNIPEFWANMWDEAGIISSKS
                     YDTVIDDITKMPGARWFSGARLNYAQNLLRFRDDKTAIVFKGEAMNEPERITYAQLFE
                     KVSGLARALRDEGIVAGDRVVGFMPNMPETIIAMLAASSIGAIWSSCSPDFGTKGALD
                     RFGQIEPKILFTASGYSYNGKRFDSLKKITEISSQLSSRPKIVVVPYTDSDQDISSVP
                     NSVHLLDFISHYKCPKIEFEQLPFDHPLYIMYSSGTTGLPKCMVQSAGGILINHIKEL
                     KLHTDVLHEDVLFYFTTCGWM"
BASE COUNT         1170 a          765 c          900 g          941 t
ORIGIN      
        1 ttgcctggaa ttgatatgta cggtatcttc atagaaaaac agcaaaaaaa gagtaaaatc
       61 gatatgcata acttgctatc agagtttttg ccttcgcgcc tggcaaagat ctggtgcgcc
      121 tcaaacatcc agtcaaaacc ggtcaaccag tataatgaaa aggaactcaa gaagattgcc
      181 catcaaatcc ataattggga aatcaaaccg gatacaaccg aagatttcaa aaccgctgag
      241 gtcacactag gcggaattga tacttccgaa ctctcatcca aaacaatgga atcaagaaat
      301 atcaaaggtc tctattttac gggcgaggtt ctggatgtga cagggcagct tgggggatac
      361 aatttgcatt gggcgtgggc ttctggtttt gtggcgggac agtatgcctg aaatagctat
      421 taaaccataa ctctgctaaa ctgaaacaaa ctccgcaaat gcgagttcgt atcagttcgt
      481 acggttagta cgagttagtt gttgatttct ttcataccaa agcacagagg aatcttggta
      541 ttcgactcaa cccaaattta ttataggcta tattaacctt cttacttata aaaaataatg
      601 ctaaaatata tacattatag ggtactattt ggatactaat cctgaaggag gtaatttaaa
      661 tggttgaaaa atttaataag cacacattgc aaatggaaaa caagaacgat tttatagcag
      721 aaacaagaat taagatctgg gggtaattta tgatacctgt tagcgcacac aaatcagtaa
      781 ttgaaatgca taatatattg cacaaagata atataacaaa ctcttttgat agggcggatg
      841 cccagggaaa acgctgttca ttctgcgaac agggcataag ctgccagctt tgcagcaacg
      901 gcccatgcag aattaaaccg ggcgcagaac gaggagtctg cggtatagat gctgacgcga
      961 tggcaatgag aaacatgatg ttcctgaaca ccatgggtac agcaatatat acatatcatg
     1021 caaagagtgt cgcaaaaaca ctcaaagcta ccgcattggg taaaacacct ttcaagatca
     1081 cggacgaagc aaagcttcga agttttgccg ggaagctggg ggtaaaaact gacggtagca
     1141 caaaagaaat tgcacttgca ttggcagatg cgatgacagc tgaaataaac tccgattctg
     1201 acatcgagct ttccaatgtt cttcgttttg ccccaaagtc aagaatcgaa ctctggagaa
     1261 aacttggcat ccttcccgga ggaccgcttc atgaagtcat ggattctgtt tcgagcacta
     1321 tgacaaatgt ggacggcgat tatgtttcgc ttgcaaaaaa ggctttgcgc cttggaattt
     1381 cctgcatata cggctcacag ataccacttg agatgggaca ggatatactt ttcgggacac
     1441 ctcaaccgca tgcggtcaat gttgatcttg gaattatcga tcctgcttac gtaaatattg
     1501 cggcgaatgg tcatgagcct tttatgggcg ctgcgctcat caaagctgcc catgaggcaa
     1561 aaaaccagga aactgccagg aaagcagggg caaaaggact gcatattatc ggctcaatcg
     1621 aaacaggaca ggaacttgtc cagagataca atatagacga tgtgttcgtt ggcatgacag
     1681 ggaactggct atccatcgaa cccgcacttg caacaggcgg catcgatgtt tttgccatgg
     1741 atatgaactg ctcacctccg ggaatgggag aataccagga taaatacaac actaccctta
     1801 tttcggtttc aaaactcgtt aatgtcccgg gtatgaaaga ccagatggcc tataaaccgg
     1861 aagatgcagc agcccaggca cagaaactca tagatatggc gattgagaac ttcaagaaaa
     1921 gaaaaggaaa agtaacaaat ccttcaagca ggaagcagaa cgctgttatc ggtttctcga
     1981 ctgaggcgtg tctcggggcg ctggggggca cccttgatcc gttgcttgat gttatcaaga
     2041 agggcacttt gaaaggagtc gtggcgcttg tcagttgtac cacgctgcga gatgggggac
     2101 aggacatcaa taccctgaag atggcccgcg aacttatcaa aaaggatata ctggtcataa
     2161 gcgcaggttg cggtaatgct gcccttcagg tgggaggact tacaaccctt gaagcgaagg
     2221 aagaagctgg ctccggtcta aaggcggtat gtgaattgct caaaattccg ccggtgttga
     2281 gctttggcac atgcacggat acaggaagga tagctcttct tgtcacggct gtcgctgacg
     2341 ctctgggggt tgacccgtct gcattgccag ttgcggtcac agctcctgaa tatatggaac
     2401 agaaggctac gatcgatgcc atttttgcgc tagcttttgg cctgtataca catgttgcac
     2461 cccttccgcc ggtagcagga gctccacgcc ttgtgaaact acttacagtg gaccttgaag
     2521 gaataacagg cggaaagatt gctgtagaga ctgacatggt tgaagcggca aaggggatag
     2581 aagcccatat aatgaagaaa cgggctgctc tgggattgcc caattaaata actcctgcaa
     2641 acagggagaa aaatggtggg cacaggcacg gtttgaaaaa ccacccagat cacagaggaa
     2701 cacagagaaa catatctctc tctgtgaact ctgtgcccac tgaggtttat tttcgttgtc
     2761 ataggaatac catcaggcac aaatgtaata tattattgaa tattgttata taattatcat
     2821 gcgtggtgaa ctgttatgga aaccgtctga agagagaatt agaaacacga atatgcataa
     2881 atttatgcag gcagttaaca gaaaacacgg aacgaatttt aaagaatact ccgaattata
     2941 tcagtggtcg atccagaaca ttcctgagtt ctgggccaac atgtgggacg aagcaggcat
     3001 catatcctca aaaagttatg atactgtaat agacgatata acgaaaatgc ctggagcaag
     3061 gtggttttct ggcgcaaggt taaattatgc acagaatctt ttgcggttcc gggatgataa
     3121 gacagcgatt gttttcaagg gcgaggcaat gaatgaaccg gaacgaataa cgtatgccca
     3181 attattcgaa aaggtgtcag gacttgcaag agcccttcgg gatgaaggta ttgttgcagg
     3241 tgatagggtg gtgggtttca tgccaaatat gccagaaaca ataattgcta tgcttgccgc
     3301 ttcaagcatt ggggcgatat ggtcatcatg ctcacctgat tttggcacaa aaggtgcact
     3361 tgacaggttc gggcagatcg aaccaaagat actgttcact gccagcggat attcatataa
     3421 tggtaagaga tttgattcac tcaagaaaat aacagagatt tccagccaat tatcatcacg
     3481 gccaaagatc gtggttgtac cttataccga ttctgatcag gacataagct cagttccgaa
     3541 ttcggttcat cttcttgatt tcatatcaca ttataaatgt cctaagatcg aatttgagca
     3601 gctgccgttc gatcaccctc tctatataat gtattcatcg ggcacaacag gtttgcccaa
     3661 atgcatggta cagagtgcgg ggggaatatt aataaatcat atcaaggaat tgaagcttca
     3721 cacagacgta ctgcatgagg atgtattatt ttattttaca acatgcggct ggatga
//
LOCUS       DATGBN010000421         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_461233, whole genome shotgun sequence.
ACCESSION   DATGBN010000421 DATGBN010000000
VERSION     DATGBN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461233"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VKL21_10610"
     CDS             <1..829
                     /locus_tag="VKL21_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB46262.1"
                     /translation="YLKQINFMLFFSMDKQLPVLLKPLPGSVRDIKALKTVIDEVNAK
                     DSTVIMDRGFASNVIPDLLTEFDFNFILPLRRNFTEIDYDRRLDYTFSYRKRGIKWGR
                     KRIGANFLYMFEDVKLRAEEETTFIHLMNEKKRNIEEYKEESKKFGKIAILSNRDQDG
                     ETIYLMYKDRENIEVAFDALKNELENDKTYLDDDDAVRGYFFISFISLYLYYKILNLL
                     RKAKLVDKVSVNEVLLELSKVYEIHLGEKRKLSEIPEKVMKLAESLELNIFPTKLRS"
     gene            complement(810..>1741)
                     /locus_tag="VKL21_10615"
     CDS             complement(810..>1741)
                     /locus_tag="VKL21_10615"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HMB46263.1"
                     /translation="GVDVVLLDLNLPDSKGFNTFTKTLAQAPEIPIIILTGLDDETLA
                     IRAVRTGAQDYLIKKHVNSSLLVRTIRYAMARRMGGERRFTIQELKEYDGKEGKPAYA
                     VFKGKVYDISNSPLWKNGIHAGKHFAGVDLTESISSAPHSEEILIKFRIVGELGKEKS
                     FGQKLLLKLEELHPHPILVHFPIAFSIGFSLLSFLYVFTGKIPFEIGSYYMLFLGFLA
                     TPFAGISGFFSWKVTYEGKMTKIFARKIIFTVVLFAVITTCFVWRTLNPDVLVPTSLN
                     YFYLILVLSLVPIVTILGYDGGEIVYTLNSAIL"
BASE COUNT          581 a          299 c          333 g          528 t
ORIGIN      
        1 ctatttgaaa caaataaact tcatgttgtt cttttcaatg gacaaacaat tgcctgtttt
       61 gctaaagcca ttgccaggtt cagtaaggga tataaaagca ttaaagactg taattgatga
      121 agtgaatgcg aaagatagca ccgtaattat ggatagagga tttgcctcta atgtcattcc
      181 tgatttgctg acagagttcg atttcaattt tattcttcct ttgagaagaa attttacaga
      241 aatagattat gaccggagac ttgattatac tttttcatat aggaaaagag gaattaaatg
      301 gggtagaaaa cgaataggag caaattttct ttatatgttt gaggatgtga aattaagggc
      361 tgaagaagaa actactttta ttcatttgat gaacgagaaa aaaagaaaca tagaagagta
      421 taaagaagag tccaaaaaat ttggcaagat tgcaatctta tcaaacaggg atcaggatgg
      481 agagacaata tatttaatgt ataaagatag ggaaaacatc gaagttgcat ttgatgctct
      541 caaaaatgaa ctggaaaatg ataaaacata tctagacgat gatgatgcag tccgcggata
      601 tttctttatt tcattcatat ccctctatct ttattacaaa atcctcaact tattacgaaa
      661 agccaaactc gtagacaaag tttctgtaaa tgaggtttta ttggaacttt caaaagtata
      721 tgaaatccat cttggagaaa aaaggaagtt gagcgagatc ccagaaaagg ttatgaaatt
      781 ggctgaatct ttggagttga atatattccc tacaaaattg cggagttaag ggtataaact
      841 atttctcctc catcgtatcc aagaatagtg actataggta ctaaactcaa taccaatatt
      901 aaataaaaat agtttaaact tgttggtaca agtacatccg gattcagggt tcgccatacg
      961 aaacatgtag tgataacagc aaataatacc acggtaaata ttatttttcg tgcgaaaatt
     1021 ttggtcatct tcccttcgta agtgactttc catgaaaaaa agccagagat tccagcaaaa
     1081 ggagttgcta aaaatcccag gaaaagcatg taatatgatc ctatttcaaa aggaattttg
     1141 ccggtgaaaa cgtataggaa cgataatagt gaaaaaccga tagaaaaagc tatgggaaaa
     1201 tgaaccaata ttggatgagg atgcaactcc tctaatttca gcaacagttt ctgtccgaaa
     1261 gacttttcct tgcccaattc tccgacgatg cggaatttta ttaatatttc ttcgctatga
     1321 ggcgcacttg atatgctctc tgttagatca acacctgcga agtgtttgcc tgcatgaatt
     1381 ccatttttcc agagaggact gtttgaaatg tcatagactt ttcctttgaa gactgcatac
     1441 gctggttttc cttcttttcc atcatattct ttcaactcct ggattgtgaa gcgcctctct
     1501 cctcccatcc tgcgagccat ggcgtagcga atggtacgta ccagcaggct actgttcaca
     1561 tgtttcttga ttagatagtc ctgcgcgcct gttcttacag ctctgattgc aagtgtttca
     1621 tcatcaagac cagttaatat tatgatcggt atttcaggtg cttgagcaag cgtctttgta
     1681 aaagtattaa aacccttgct gtcaggtaga ttgagatcca atagaaccac atcaacccct
     1741 t
//
LOCUS       DATGBN010000422         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_499802, whole genome shotgun sequence.
ACCESSION   DATGBN010000422 DATGBN010000000
VERSION     DATGBN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499802"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /gene="eno"
                     /locus_tag="VKL21_10620"
     CDS             <1..570
                     /gene="eno"
                     /locus_tag="VKL21_10620"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307791.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMB46264.1"
                     /translation="EKAGYNKKITIGLDSAATEFYKDGKYKVDGKKFSSGELIDFYSD
                     LVKKYPILSIEDPFEEESFQDFAELTKKLKDTIIIGDDLFVTNVKRLEKGIKMKAGNA
                     LLLKVNQIGTLSEAFDAAQLAQKNRFKVVVSHRSAETEDTTIADISVAVGAEFIKTGA
                     PARSERNAKYNQLLRIEEELGNAARFVEI"
     gene            697..924
                     /locus_tag="VKL21_10625"
     CDS             697..924
                     /locus_tag="VKL21_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46265.1"
                     /translation="MSINELMLIVFLINIPFGYIRSNAAKFSRKWMMAVHIPVPFVFL
                     LRIFSGFNWTVIPLLVLSDVAGQIAGGKLRK"
     gene            complement(1249..1494)
                     /locus_tag="VKL21_10630"
     CDS             complement(1249..1494)
                     /locus_tag="VKL21_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46266.1"
                     /translation="MKSKKGRIELSYNFQITADHNGFILASDITDSSADTYQLKPMVL
                     QTEQNLEKIPEQIKWTFDNSYNEGENLKFLAPLNPFL"
     gene            complement(1515..1856)
                     /locus_tag="VKL21_10635"
     CDS             complement(1515..1856)
                     /locus_tag="VKL21_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46267.1"
                     /translation="MLDLSLLSTDGTKIKAKAADKRILTKEELEFLMKFVENELETWA
                     AQDSLEDDFFDNIRGSDQLPGSSRKRVKGVVKHYVEELKEKGEPFRTKIEAKRERAIR
                     KWKRTNLKRSA"
     gene            complement(1814..2143)
                     /locus_tag="VKL21_10640"
     CDS             complement(1814..2143)
                     /locus_tag="VKL21_10640"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMB46268.1"
                     /translation="MVFIKSFKNQTWLFPPAIEDLIPEDHVCFLVENFIDSLDLSVFE
                     DKYEGAGHPAYHPGILLKLLVMGVLDKVRSSRRLAKNARENVVYMFLAEKRECWTSPY
                     CQQMERK"
     gene            2361..>3603
                     /locus_tag="VKL21_10645"
     CDS             2361..>3603
                     /locus_tag="VKL21_10645"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HMB46269.1"
                     /translation="MNSQHINICANGMINVTRFRIYPNKEVEDKLFSNLHICGFVYNW
                     CLDHNIWNDSVLPQLKEVYPDIKEVHSIVLQNVVHQIRDNIKALSEKKKKGRKVGRLR
                     HKPRYSMIYEQSGFRIDGDLLRLSKIGAIPIVLCRPIYGTIKQIIIKHNKTHNWFAIL
                     VSEDKIKEWYEFRRSYQKPGNSIKSVGIDLNVSNFSTDSDGLVVEQPKNVKKAENKLK
                     RCQRKLSGKSRGSNNRKKQRRIVAKVHEKIENRRSDFLHKTSRYYVNNYDLIALEDLS
                     IAGMVQSGNSGLSKAILDASWGKFANYVLYKAENAGKYAVQVIAKGTTQDCSVCGKTV
                     PKDLSERIHSCPYCGSIMPRDYNASQNIKFRGIKMVGWGTPEPGFLDIEKLNTLAEIK
                     TSILDINNPEQVFVNEPRISRL"
BASE COUNT         1171 a          639 c          760 g         1033 t
ORIGIN      
        1 gaaaaagcag gatataataa gaagataacg atcggtcttg attctgctgc cacagagttc
       61 tataaggatg gtaaatacaa agttgatggc aagaaattct caagcgggga attaatagat
      121 ttttattccg accttgtcaa aaaatatccg atcctttcca tcgaagatcc tttcgaggag
      181 gaatcattcc aggattttgc ggaattgaca aaaaaattga aggatacgat aatcataggc
      241 gacgaccttt tcgtgacgaa tgtaaagcgg cttgagaagg ggataaaaat gaaagcggga
      301 aatgcgctgc ttcttaaagt gaaccagata ggcacgttgt cagaggcatt tgatgcggcg
      361 cagcttgcgc aaaagaacag gttcaaggtc gtagtaagcc acaggtcagc agaaaccgag
      421 gacaccacta ttgcggatat ctcggttgcg gttggcgctg agtttataaa gaccggcgcg
      481 ccggcaagga gcgagaggaa cgccaagtat aaccagttgc tgaggataga ggaggaattg
      541 gggaacgcgg cgcggtttgt ggagatatga gtatttaatt tctcatacca gtgataaccc
      601 tcaggaatat ggcaaatccg gaccactatc atttccggac caccacaaac tacttatgta
      661 aagaatagcc tacaaacatt aggataggag aaaaaaatgt caataaatga actgatgtta
      721 atagttttct taataaacat tccgtttgga tatattcgaa gcaacgctgc aaagttctca
      781 agaaaatgga tgatggcggt tcatatcccg gttccatttg ttttcttgtt aaggatattc
      841 tcaggtttta actggactgt cattccgttg ctagtattat ctgatgtagc tggacagatc
      901 gctggtggaa aattaagaaa atgatataca taagataggt aggtgggcag aaaaggggat
      961 tatggtggta tggaaaaaat atttttctct gcccactaca attcgtatat atacgaacta
     1021 aagtatttat agtttacggt tatcgtaaac ttgggtatca ttttacaaaa aacagatgat
     1081 taggaggtag tgggcttttg ggatttatgg tggtgtggta tcgaaaaaaa atattttcta
     1141 tgcccactac attcgtaaat atgcgaacta aagtatttat agtttacggt tataattatt
     1201 atgagcatca atatctttta tcatatccgt tacggaattc ctttaaattt acagaaatgg
     1261 gttcaaaggg gcaagaaatt tcagattttc accctcgtta taactgttat caaaggtcca
     1321 ttttatctgt tctggaattt tctcaaggtt ctgctctgtc tgaagaacca ttggcttcaa
     1381 ttgatacgta tcagcggatg aatcggtgat atcactcgca agaataaatc cattatgatc
     1441 tgctgtgatt tgaaaattat aagagagctc tatcctccct ttttttgatt tcataaacct
     1501 gcattccgga tcagtcatgc tgaccttttc aagttcgttc ttttccactt cctgatggct
     1561 ctttcccgtt ttgcctctat cttcgtcctg aatggttcac ctttctcttt taactcttca
     1621 acgtagtgtt ttacgacacc tttgactcgc tttctggatg aacctggaag ctggtcgctg
     1681 cctcttatat tatcaaaaaa atcatcttca agcgagtcct gagcagccca agtttcaagt
     1741 tcattttcca caaatttcat caggaattca agctcttctt ttgttaatat tcttttatcc
     1801 gcagcctttg cttttatttt cgttccatct gttgacagta gggagaggtc caacattccc
     1861 tcttttctgc aagaaacata taaaccacgt tttctctggc attttttgca agccttctgg
     1921 aagatctgac tttatcaagt actcccatga caagcaattt gaggagaatg cctggatggt
     1981 atgcaggatg tcctgctcct tcatatttgt cttcgaatac tgaaagatca agtgaatcaa
     2041 tgaagttttc aacaaggaaa caaacatggt cttcgggtat caggtcttct attgccggag
     2101 ggaacagcca ggtctggttt ttgaaggatt taataaatac catattacca atacagtaat
     2161 tatatgatat aaatgtattg ataattaatc gtaagcttta gacactatta taacagtata
     2221 aaataaatag attagattaa attcaatcaa ttgtcggaca gcctgttcag tcgtggagat
     2281 gaattgcccc tttgcatgaa cacgatgctc acccaaaaag catttgcgcg aacggatttc
     2341 agggtgagca aaatatatat atgaactcac agcatattaa tatttgcgca aacggcatga
     2401 taaatgtaac aagattcagg atatatccga acaaggaagt tgaagataaa ctcttttcta
     2461 atcttcatat ttgcggtttt gtctataatt ggtgcttgga tcataacatc tggaatgatt
     2521 ctgtattgcc tcaactcaag gaagtgtacc cggatatcaa agaagttcac agcatagtgc
     2581 ttcagaatgt ggttcatcag atcagggata acataaaagc tctttcagaa aagaaaaaga
     2641 aaggtcggaa agtaggtaga ttgagacaca aaccaagata cagcatgatc tatgagcaga
     2701 gcgggttccg gattgatgga gatttgctaa ggctcagcaa aataggagcc atcccaatag
     2761 ttctgtgcag accgatatat ggaactatca agcagataat aatcaagcac aacaagacac
     2821 ataactggtt tgcaatatta gtttctgagg ataaaatcaa ggaatggtat gaatttcgca
     2881 ggtcatatca gaaaccagga aatagcataa aatccgtggg aattgatctc aatgtatcta
     2941 atttctcaac agatagcgat ggacttgttg tagagcagcc gaagaatgtc aagaaagcag
     3001 aaaataaatt gaaaagatgt cagaggaaac tatccggaaa atcgagaggt agtaataaca
     3061 ggaagaagca gagacggata gtagcaaagg tacatgaaaa aatcgaaaat agaagaagtg
     3121 atttcctgca taaaacaagc aggtattatg ttaataacta tgacctcata gctcttgaag
     3181 atttaagcat agctggaatg gttcaaagtg gaaacagcgg actatcaaaa gctatccttg
     3241 atgcttcatg gggtaaattt gcaaactatg tattatacaa agccgagaac gctggtaaat
     3301 atgcagttca agtcatagca aaaggaacta cacaggattg tagtgtatgc ggcaagactg
     3361 taccgaaaga cctttcagaa aggatacatt catgtcctta ttgcggttct atcatgccga
     3421 gagactacaa tgcatctcaa aatatcaagt tcagaggaat aaaaatggta gggtggggca
     3481 cacccgaacc aggctttctc gatatagaga agctaaatac gcttgcagag ataaaaacct
     3541 ctattctgga tatcaacaat ccagaacaag tttttgtcaa cgaaccaaga atctcccggc
     3601 ttt
//
LOCUS       DATGBN010000423         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_189514, whole genome shotgun sequence.
ACCESSION   DATGBN010000423 DATGBN010000000
VERSION     DATGBN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189514"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VKL21_10650"
     CDS             complement(<1..1075)
                     /locus_tag="VKL21_10650"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HMB46270.1"
                     /translation="MAKVKLTDTTLRDAHQSLIATRMRTRDMLPIAEKMDDVGFFSLE
                     VWGGATFDSSIRYLNEDPWDRLRALKKTIKKTPLQMLLRGQNLVGYRHYSDDIVVKFV
                     EHAAATGVDIFRIFDAVNDIRNMEVSIRTAKRVGAHVQGTVCYTISPVHPIPLYVKMA
                     KELEEMGCDSLCIKDMAGLISPQNAFDLVSALKEEISIPIDLHSHCTSGMAPMSYILA
                     CQAGVDVLDTAFSPFAWGTSQPPTETTVAALRGTPYDTGLDLFLLTEIKKYFEDIKEK
                     YRGILDPISERIDTNVLKYQIPGGMLSNLVSQLKEQNALDKYEAVLAEMPRVREELGY
                     PPLVTPTSQIVGTQAVLNVLMGER"
     gene            1165..1503
                     /locus_tag="VKL21_10655"
     CDS             1165..1503
                     /locus_tag="VKL21_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46271.1"
                     /translation="MARKLRSSDLLILKKIAPELVDSTCGHAGWEFRSVLPPVSQHIS
                     TSNSDFRERLLKLSHEELIYLVDLVFEGMESLHCVKKEHVDAFIEVVSERISKVKAAE
                     LLELYEFERS"
     gene            1484..2062
                     /locus_tag="VKL21_10660"
     CDS             1484..2062
                     /locus_tag="VKL21_10660"
                     /inference="COORDINATES: protein motif:HMM:NF021398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HMB46272.1"
                     /translation="MNSRGVEMAHIMELFGDNPLLVQGMGTLMSGLIETEPIPEIIAG
                     IESRGGIVLDRENAGIDQAGGLIKACELGFKKIAVSVVSPGEAGKLRLIEKENDIDLI
                     LIGAHLTGIKCKEAEELIQKMDIITGCASNMVRRLIHPLLQAGTSVPVFAMTRKGKEL
                     IIERAKEIDSPLLVSSAELPVLPEHKQPRPLR"
     gene            2205..2375
                     /locus_tag="VKL21_10665"
     CDS             2205..2375
                     /locus_tag="VKL21_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46273.1"
                     /translation="MIQESLLRNRRPRQDIPTKWKEIIKQEYEMFRLNALYQEKTIYA
                     RCKIRTPVGGII"
     gene            2398..>2730
                     /locus_tag="VKL21_10670"
     CDS             2398..>2730
                     /locus_tag="VKL21_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HMB46274.1"
                     /translation="MKVLNEAIERNSHLYPIMSIISDHGSQFYANKRDKNGYADHAFE
                     IFLKDKGIKQILCGVNHPQTNGKQEKFHDFYKNHRGRFESLDKMIEWYNNRPHGSLNL
                     RRAETPNQA"
BASE COUNT          768 a          571 c          655 g          736 t
ORIGIN      
        1 agcgttcacc catcaatacg ttaagcactg cctgcgtgcc cactatctgg ctcgtgggag
       61 tgacaagcgg gggataaccc agttcttcgc gcacacgggg catctccgca aggactgcct
      121 catatttatc cagggcattc tgctccttca actgggatac aaggttggag agcataccgc
      181 ctggtatctg gtattttaag acattcgtgt ctattctctc tgagatcggg tcaaggatgc
      241 ccctgtattt ttctttaata tcttcaaaat atttctttat ctccgtgaga agaaaaagat
      301 caagaccggt atcgtaagga gttccgcgaa gcgctgccac tgttgtttcc gttgggggct
      361 gcgatgtgcc ccaggcaaat ggtgaaaaag cagtatcaag tacatcaacc cctgcctgac
      421 atgcaagaat gtagctcatt ggagccattc cgcttgtgca gtgactgtgc agatcaatag
      481 ggatgctgat ctcttctttg agggcgctga caagatcaaa agcgttctgc ggcgaaataa
      541 gacctgccat gtcttttatg caaagtgagt cgcatcccat ttcttcaagt tcctttgcca
      601 tctttacata aagggggatt ggatggaccg ggctgatagt ataacaaacg gttccctgta
      661 catgtgcccc aacgcgcttt gctgtcctga tagatacttc catgttcctg atatcgttca
      721 cggcatcgaa tatcctgaaa atatcaacac ctgtcgctgc tgcatgttcc acgaatttga
      781 ctactatatc atcggaataa tgcctgtagc ctaccaggtt ctggccgcga aggagcatct
      841 ggaggggtgt tttcttaatg gtcttcttaa gcgcccggag cctgtcccat gggtcttcat
      901 taagatatct tatcgaggaa tcaaaagtcg ctcctcccca cacttccagt gaaaagaatc
      961 ccacatcgtc catcttctcg gctatgggga gcatatccct tgtgcgcatg cgtgtcgcta
     1021 tcagtgattg gtgggcgtct ctcagggtcg tatccgtaag ttttactttg gccatgattt
     1081 atgtggcttt attagttttg gtgttattta aattatggtt caggtaatga tttaacttca
     1141 aaggaagatt taatggatta aacaatggca cgaaagctca ggtcttcgga tttgctgata
     1201 cttaagaaaa ttgcgcctga acttgtggat agcacatgcg ggcatgcggg ctgggaattc
     1261 aggtcagtgc tccctcctgt gagccagcat atttctacct caaattcgga tttcagggaa
     1321 cgtttattga aactgtcaca tgaagagctg atctatcttg tggatctggt ctttgaggga
     1381 atggaaagcc ttcattgcgt aaagaaggag catgtggatg cttttataga ggtggtaagt
     1441 gaaaggatct cgaaagtaaa agctgccgaa ttgcttgaac tttatgaatt cgagaggagt
     1501 tgaaatggcg catattatgg aacttttcgg cgataatcct ttgctggtgc agggaatggg
     1561 cactttgatg tcaggactta ttgaaaccga gccgatcccg gaaattattg ccgggatcga
     1621 atcgcgggga ggcattgttc ttgacaggga aaatgcaggg atagaccagg caggcggtct
     1681 tattaaagcc tgtgaacttg gttttaagaa aattgcggtc agtgttgtct ctccgggaga
     1741 ggctggaaaa cttcgtttga tcgagaaaga aaatgacatt gatcttatcc tgatcggcgc
     1801 tcaccttaca ggcattaaat gcaaggaggc cgaagagctg attcaaaaaa tggacattat
     1861 cacaggctgc gcttcaaaca tggtgcgccg cctcatacat cctcttctcc aggccgggac
     1921 ttctgttcca gtcttcgcga tgacccggaa aggaaaagag ctgataattg agagagcaaa
     1981 ggaaattgat tcaccactcc tcgtcagctc agcagaattg cctgtgcttc cggagcataa
     2041 gcagcccaga ccgctaagat aatgaacctg acgtttgatc ctccgatgaa ctttccaggc
     2101 tgaagtttgt atcttttccc aaaaaaattt cctcattcta aacaaatgta gatatgtggt
     2161 aaaaatcaat aacaagcgaa agctcaaata ttatttgaag gattatgatt caggaaagtc
     2221 ttctcagaaa tcggcggcca aggcaggata ttccgacaaa atggaaggaa atcatcaagc
     2281 aggaatatga aatgtttcga ctgaatgcgt tgtatcagga aaaaacaatt tatgcaagat
     2341 gcaaaataag aactcctgtc ggcgggataa tttgacaacg cgactgaaga gaactctttg
     2401 aaagtcctga atgaagcaat tgaaagaaat agtcatctgt atccgattat gtccataata
     2461 tccgaccatg ggtcgcaatt ttacgccaac aaacgagata aaaacggtta tgcagatcac
     2521 gcatttgaaa tatttcttaa ggataaggga atcaaacaga tcttatgcgg tgtaaatcat
     2581 ccgcagacta atggaaaaca ggagaagttt cacgattttt acaagaacca caggggaaga
     2641 tttgaaagtc tggataaaat gatcgagtgg tacaataatc gccctcacgg atctttgaat
     2701 ttaagacggg cagaaacgcc aaaccaggca
//
LOCUS       DATGBN010000424         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_461374, whole genome shotgun sequence.
ACCESSION   DATGBN010000424 DATGBN010000000
VERSION     DATGBN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461374"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VKL21_10675"
     CDS             <1..660
                     /locus_tag="VKL21_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMB46275.1"
                     /translation="VLVVEETEPVIERQFSKRVLGKLTGHMPYGIVEIGDIRKALDNI
                     NIDAVKPDIVPQTIEKRGDRPICEDCPYLPMYLAIKELDVPVAGDLGCSIMTSSPPFS
                     LIDAAFSLGSSISAACGFNRKGIAIIGDYGLAHSGIPALINAVHNEHEVLVVVLQNEV
                     AAMTGGQKVPDLTGLLNYYIKDTATVDPTNQTIIKSLLETKIKNRGISILIAKARCPR
                     Y"
     gene            743..2944
                     /locus_tag="VKL21_10680"
     CDS             743..2944
                     /locus_tag="VKL21_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HMB46276.1"
                     /translation="MDDRIMLRVAEAHHRDVGRDIARIDRDIMEQMGILSGDIIMVVG
                     KEKACAIAGPGYPEDNGMDLIRIDGNIRGNARVGIDDKIYLQKSTPQIAKRVVLAPTE
                     QIRLVGGPAYLIRLLEGRPVAKGQRLRVETVSNPLSFIVISTQPPSPVIVSRTTSLVI
                     KEEVIEELEVRQTHLTYEDIGGLRREIGLIREMIELPLRHPELFEKLGIEPPKGVMLH
                     GPPGTGKTMIAKAVANETDANFVSISGPEIMSKFYGESEKHIREIFEEAEKNAPTIIF
                     IDEIDSIAPKREEVTGEVERRVVAQLLSLMDGLKKRGQVIVIAATNRPNAVDAALRRG
                     GRFDREIEIGIPDRLGRFDVLQIHTRGMPLAEDMSEEKGLREIADMTHGFVGADLSSL
                     CKEAAMHAIRKILPKINIEEEIPEEIMEQIFVTREDFQNALQNIEPSALREVFIEVPA
                     VRWTDIGGLESVKQELIEAVEWPLKFPEAFEAINTRPPRGVLLFGPPGTGKTMLAKAI
                     ATESEANFISIKGPELLSKYIGESEKAIRETFRKARQAAPTIIFFDEIDSMVPTRGSA
                     FDSGVTERMVSQILTEIDGLEELKNVVVVAATNRPELVDPALLRPGRLERFIYIRPPD
                     LKEREIIFNIHLKGKPLASGIDSKELAQKTQGYVGADIEAICHEAAIIALRDWIKPGM
                     DRETVKKEVLNVRIQEEHFEKAFTMVRPTQWDQKEYEEMADITKAINRNNK"
     gene            2956..3405
                     /locus_tag="VKL21_10685"
     CDS             2956..3405
                     /locus_tag="VKL21_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMB46277.1"
                     /translation="MKKRNDFEEFLEKLEETLNNIMDEIDFPEKKPVNISLSVNIMPV
                     HANNPGEIFITRKDKTPVDILETNKNIHVVIGLSGIEPEDIMLTCSGNALEITANNLE
                     RTVNEFIELPARVNKTGIKTTYNNGILEVIFNKTRLLGKSKYSQQSF"
     gene            3437..4309
                     /gene="hisG"
                     /locus_tag="VKL21_10690"
     CDS             3437..4309
                     /gene="hisG"
                     /locus_tag="VKL21_10690"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HMB46278.1"
                     /translation="MLDIALPKGSLEEQTLLLFKQADLEIRKTDREYNPTVKDPRIRK
                     VKILRPQEIPKYVEEGYFDLGISGKDWVIESDSDVVEVADMFYSKMGEGIVKIVIAVP
                     NEKDIKSAKDIKPGSRVSTEYPNLTKKFFNKLGIPVDLRYSYGATEAKVPELVDVIVD
                     LTETGSTLRKNGLKIVDIILESNTKLIANKKSMNDPVKRKEIEEIKILLQAVLEARGK
                     VFIVMNVPENKLDAVVSGLPAMKRPTVSKLYKSDYYAVQTVVSKNEINILIPQLKALG
                     AEDILEMDIMKIVH"
     gene            4317..5258
                     /locus_tag="VKL21_10695"
     CDS             4317..5258
                     /locus_tag="VKL21_10695"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HMB46279.1"
                     /translation="MLKITTPSRLHLTLIDLNASIGRIDGSIGITLDNPVIKIEGKKF
                     DIVEITGCSEHIGRMGNSVKALLPDGEGIKISIEKDYPTHIGLGSGTQAALAAGMAVN
                     ELYDLGLNVHGLAVKVGRGGTSGIGVAAFERGGFILDGGHKFSEKKAFLPSAASKLPP
                     APVLLRSEFPDWDILLAVPEQKGASQKNEVNIFQEKCPIPLREIEALSHVILMQLLPS
                     LFEKDIVTFGKSINAIQELGFKKREVELQSVSKDVMQALRDGGAHGAGMSSFGPTVYA
                     FGEDMKNLEKIASDLLGGKGKVFRTKARNKGAIIRTL"
BASE COUNT         1771 a         1095 c         1358 g         1319 t
ORIGIN      
        1 gtacttgttg tagaagagac agagccggtt attgagagac agttctcaaa aagagtattg
       61 gggaaactca caggacatat gccttatggc attgtagaaa ttggggatat ccgaaaagct
      121 cttgataata tcaatattga tgctgtaaaa ccagacattg taccccagac catagaaaaa
      181 cgaggcgatc ggcccatttg cgaagactgc ccctatctgc caatgtatct ggcaataaaa
      241 gaactggatg tcccggtagc aggcgacctt ggatgttcca tcatgacctc atcgccgcca
      301 ttttcattaa ttgacgcagc attttcactt ggctcatcca taagcgctgc ctgcggattc
      361 aacaggaaag gaatcgcgat aataggtgat tacggccttg cccattcagg gatccctgct
      421 cttatcaatg cagtccacaa tgaacatgaa gtccttgtag tcgttcttca gaatgaagtt
      481 gcagccatga ccggagggca gaaagttccg gacctgaccg ggcttttgaa ttattatatc
      541 aaggatactg ctactgtgga tcccacaaac caaactatta taaaaagctt attggaaaca
      601 aaaataaaaa acaggggaat ttctattttg atcgccaaag caagatgtcc cagatactaa
      661 aaaaggaaat tacacgatcg tatccttttc ttcacatcgg gttttaagta ttattacgac
      721 taaaacataa tcaggtatca caatggacga tagaataatg ctgcgggttg ccgaagccca
      781 ccacagggac gtgggaaggg atattgcacg aattgatcgc gacatcatgg aacaaatggg
      841 tatcttaagc ggagatatta ttatggtcgt gggcaaggaa aaagcgtgcg ctatcgcagg
      901 accgggttat cctgaggaca atggaatgga tttgatcagg atagatggga acatcagagg
      961 aaatgcgcgt gtggggatcg atgataaaat atatctccag aaatccacac cacagatcgc
     1021 aaagagagta gttcttgccc cgaccgagca gatccgcctt gtaggaggtc ctgcgtatct
     1081 gatacggctt cttgaaggca gacccgtcgc aaaaggacag cgcctgcggg ttgagaccgt
     1141 aagcaatcct ttgtccttca ttgtcatatc aacgcagccc ccaagccccg ttattgtgtc
     1201 aaggaccaca agtcttgtca taaaagaaga ggtcatagaa gaacttgaag tgcgccagac
     1261 gcatcttacc tatgaagata tcggaggctt gcggcgcgag atcggcctta tcagggagat
     1321 gatcgaactt cctcttcggc atcctgaact tttcgagaaa ctgggtatcg agcccccgaa
     1381 aggtgttatg cttcacgggc cgccggggac tggcaagacc atgatcgcca aagcagtggc
     1441 caatgaaact gatgcgaatt ttgtttccat aagcggccct gagataatga gtaaattcta
     1501 tggggaatcc gagaagcata tccgcgaaat attcgaagaa gcagaaaaga acgctccaac
     1561 tatcattttc atcgatgaga tagacagtat agccccaaaa cgggaagaag tcacagggga
     1621 agtcgaaagg cgtgtggtcg cacagcttct gtccttaatg gacggcctaa aaaaacgcgg
     1681 ccaggtgatc gtcattgccg ccacgaacag gccaaatgca gttgatgcag cgttacggcg
     1741 cggggggcga tttgaccgtg agatcgaaat aggcatcccg gacaggctgg gacggttcga
     1801 tgttctccag atccacacaa gaggtatgcc gcttgccgag gatatgtctg aagaaaaagg
     1861 gctgcgcgag atcgctgata tgacgcatgg ttttgtgggc gctgacctgt catcgctatg
     1921 caaggaagcg gcaatgcacg cgatccggaa gattcttccg aaaatcaata tcgaagaaga
     1981 gatccccgaa gaaataatgg aacaaatatt cgtgacaagg gaggatttcc agaacgcgct
     2041 ccagaatatc gaaccctctg cccttcgaga agtgttcatc gaagttcccg ctgtcaggtg
     2101 gaccgatata ggtggattgg aatcagtcaa gcaggaactc atagaagctg ttgaatggcc
     2161 ccttaaattt cctgaagcct ttgaagcaat taatacaaga cctcccaggg gggtgcttct
     2221 ttttggccct ccgggaacgg gtaagaccat gcttgcaaaa gcgatagcta ccgaaagcga
     2281 agcaaatttc ataagtataa aaggacctga actgttaagt aaatatattg gggaatcgga
     2341 gaaagccatc agggaaacat tcaggaaggc aaggcaggct gcgcccacaa taatattttt
     2401 tgatgagata gactctatgg tgccaactcg aggaagtgca tttgattcag gggttacgga
     2461 gagaatggtg agccagatat tgaccgagat cgatgggctt gaagaactca agaacgtggt
     2521 cgtggttgcg gcaacaaacc gcccggaatt ggtcgatcct gcgctgcttc gcccgggaag
     2581 gctggagagg ttcatctata tccgtccacc tgacctgaaa gagcgggaaa tcatattcaa
     2641 tatccatctt aaagggaaac cgcttgcctc tggaattgat tcaaaggagc ttgcccaaaa
     2701 gacacaggga tatgtaggcg cagacatcga ggcaatatgc catgaagctg ccataatcgc
     2761 tcttcgcgac tggataaaac ccggaatgga cagggaaaca gttaagaaag aggtcttaaa
     2821 tgtcaggata caggaagagc attttgaaaa agctttcact atggtaagac ccactcaatg
     2881 ggatcaaaaa gagtatgaag aaatggctga catcacaaaa gctatcaata ggaacaataa
     2941 gtagaagata taaacatgaa aaaaagaaat gatttcgagg aattccttga aaaattggaa
     3001 gagacattga ataatattat ggatgagata gatttcccgg aaaagaaacc ggtaaacatc
     3061 tccctatctg taaacattat gccggttcat gctaataatc ccggtgagat ttttattaca
     3121 agaaaagata aaactccagt ggatattctt gaaactaata aaaacataca tgtcgtaata
     3181 ggactttcgg gaatcgaacc tgaagacata atgctcacat gttcgggaaa tgctcttgag
     3241 atcacagcaa ataatcttga aagaacagta aatgaattca tagaattacc ggcaagagtc
     3301 aataaaaccg gaatcaaaac cacatataac aacggtattc ttgaagtgat ctttaataag
     3361 accagactgc tcgggaagag caaatatagt cagcaaagct tttaatccgt gaatccattt
     3421 aggaaaccga attattatgt tagatatcgc tctcccaaag ggaagtcttg aagaacagac
     3481 cctcctttta tttaaacagg cagaccttga gataaggaag acagaccgtg agtataatcc
     3541 tactgtaaaa gaccccagga taagaaaggt aaagatacta agaccccagg aaattcccaa
     3601 atacgttgaa gaaggttatt ttgacctggg catatccgga aaggactggg ttatcgaatc
     3661 tgattcagat gttgtggaag ttgccgatat gttctatagc aagatggggg agggcattgt
     3721 taagatagtg atcgcggttc cgaatgaaaa ggacatcaaa agcgcaaagg atataaaacc
     3781 gggaagcagg gtatcgaccg aatacccgaa tctcacaaag aaatttttta ataagctggg
     3841 gatacctgtt gatcttcgtt attcctacgg cgccacggag gcaaaagtcc ctgagcttgt
     3901 cgatgtaatt gtagatttga ccgaaacggg ctcaacactg cgaaagaacg gattgaaaat
     3961 cgttgatatc atccttgaat ccaatacaaa gctcattgcc aataagaaat caatgaatga
     4021 ccctgtgaaa cgaaaagaga tagaagagat aaagatactt ttgcaggcag tacttgaggc
     4081 ccgcggtaaa gtatttatcg ttatgaatgt ccctgaaaac aaacttgatg cagtagtaag
     4141 cggtcttccc gcaatgaaaa ggcctactgt ctccaaactt tataaatcag attattatgc
     4201 ggtccaaacc gtggtaagca agaacgaaat caatattctt attcctcaac tcaaggctct
     4261 tggcgccgag gatattcttg aaatggacat tatgaagatc gttcattaac ttgaatatgc
     4321 tcaaaatcac aactccctct cgcctgcatt tgacccttat cgatttgaat gcaagcatcg
     4381 ggcgcattga tggcagcatt ggtataactc ttgataatcc cgttatcaaa attgaaggta
     4441 agaaatttga tattgttgag ataacagggt gcagcgagca tattggtcgt atggggaata
     4501 gcgtaaaggc tttgcttccc gatggcgagg ggataaaaat ctccatcgag aaagattatc
     4561 caacacatat aggtttaggt tcaggcacac aggcagctct tgcggcaggc atggccgtaa
     4621 atgaattgta cgaccttggc ttgaatgttc atggccttgc agtaaaagtg ggacggggag
     4681 gaacaagcgg cattggtgtg gctgcttttg agagaggcgg cttcatcctt gatgggggtc
     4741 ataaattcag tgaaaaaaaa gcgtttcttc cctcagcggc aagcaagctt cctccagcgc
     4801 ctgttttatt gagaagtgag ttcccggatt gggatatttt actggctgta cctgaacaaa
     4861 aaggcgcatc acaaaaaaat gaagtgaata tattccagga gaaatgccca ataccgttac
     4921 gtgaaattga ggcgctatct catgtgatcc tgatgcagct tcttccctca cttttcgaga
     4981 aggacatcgt caccttcggg aaaagtatca atgcgatcca ggaactgggg tttaaaaaac
     5041 gggaagtaga actgcagtcg gtttcaaagg atgtgatgca ggctttaaga gatggcggcg
     5101 cacatggcgc cggaatgagc tcattcgggc caacggtgta tgcttttggg gaggatatga
     5161 aaaaccttga gaaaattgcc agtgatctcc tgggcggtaa aggtaaggta ttcagaacaa
     5221 aggcaagaaa caaaggggca attatccgga ctctttaacc tgggattaga aggcaggcac
     5281 aggaaaatgg aaacttaacc cccataattt atatacatga taagattaac aatgaatcgg
     5341 tggtagaaat ggatttcctt gaaaaagaaa cgagaaggaa agattaaaaa tgaataaaat
     5401 agatagtttt atgatatgtt tgattttatt ttctttcatg ccgtcattag tggaggcaaa
     5461 aactgccgat gaatggtata atgaaggagt ttattcaggt caaactggaa agtataatga
     5521 agcgatcata gcttatgatg aag
//
LOCUS       DATGBN010000425         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_305979, whole genome shotgun sequence.
ACCESSION   DATGBN010000425 DATGBN010000000
VERSION     DATGBN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305979"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..558
                     /locus_tag="VKL21_10700"
     CDS             271..558
                     /locus_tag="VKL21_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46280.1"
                     /translation="MSKQQHDIMRKSSICNQIADTNRTKNTFATPELVGRRVAFQILI
                     NGKKGGRARPYLMYDIGTVKEVRNSAVIIEGNRGQLYNRPVDKITLLEAGR"
     gene            563..808
                     /locus_tag="VKL21_10705"
     CDS             563..808
                     /locus_tag="VKL21_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46281.1"
                     /translation="MILQTSEERIKLLKSGFTGDQVEALYIDLNHFETVGVNWGDFAM
                     NRSSSHIFARKTRLSRRGPVLQRHLSILYYGHWEHCT"
     gene            961..1113
                     /locus_tag="VKL21_10710"
     CDS             961..1113
                     /locus_tag="VKL21_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46282.1"
                     /translation="MKINGRKEEKIYVMSFGTIALKNHVILLVQESLKLKRGGHDLRT
                     VSWRGT"
     gene            1258..1722
                     /locus_tag="VKL21_10715"
     CDS             1258..1722
                     /locus_tag="VKL21_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46283.1"
                     /translation="MISREKIEKRILEEYENFVFQGYSTEEISYMGTCLQKWAIEKRI
                     GPANIEPLSQIKPTPDYWLKPLIVPVNTNTLTAMAVHGALCLALRHPQFNGDGKHGHE
                     LSRKMVVEFTKSLGEWLVHAGALTPEQLRSVEELESKEGSSDLIHEAKPGVQ"
     gene            1719..1973
                     /locus_tag="VKL21_10720"
     CDS             1719..1973
                     /locus_tag="VKL21_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46284.1"
                     /translation="MMRFTIEGTNGEIKEFISDIAQKPRDLVLPEGPISEHGYGEKIF
                     QRLEEKLSKKDSVPISAQASDKDLDRIEPDHHLDVYGGVD"
     gene            1973..2395
                     /locus_tag="VKL21_10725"
     CDS             1973..2395
                     /locus_tag="VKL21_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46285.1"
                     /translation="MADNAIIVIDGIRILSDLESEYERKHSEWKIQCSAVHARFKKKT
                     SRTKKKGKEYEYTIWYEEQPGDGLKSIGKEEPDYSKYYPPEPKPSGFIEFQEYVQEYE
                     GHLLLTERYYQNYKTIFKDCLVFRLAECLNNNHPLIGR"
     gene            2414..2644
                     /locus_tag="VKL21_10730"
     CDS             2414..2644
                     /locus_tag="VKL21_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46286.1"
                     /translation="MGIIPLSVEIKEGMKVAFFVSIKRHRARKKEVHIGEVRSFNGIG
                     VIISGITMPGIYNRAPDKLFEVHKDLSLGAFK"
     gene            2641..2817
                     /locus_tag="VKL21_10735"
     CDS             2641..2817
                     /locus_tag="VKL21_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46287.1"
                     /translation="MNRLIFKDRRGDEYVFIRYSTNEIKATKKSQLSEGYHQIDFSAE
                     AIEKLHELLHQEER"
     gene            3656..4126
                     /locus_tag="VKL21_10740"
     CDS             3656..4126
                     /locus_tag="VKL21_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46288.1"
                     /translation="MNKKERDQIYELKYRKARIDKHDTPVNKFLDKAIEEKKSVTRAI
                     HELLDRETLSQAQLRRNEQDKMEEALKLCSVIFKNILSIYRAQQLELWLITFKRVFMI
                     LDKAAAEGAQASKEDDKEYLQRAVEAIKEMERDNTEKHLQNAIPINRLNNAYKK"
     gene            4162..>4402
                     /locus_tag="VKL21_10745"
     CDS             4162..>4402
                     /locus_tag="VKL21_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46289.1"
                     /translation="MGRKRKESKPPELYVCVDCGKQAEKGYMKLVSRKIELYPCGLPQ
                     IDGNILYTTEEMDHAQKICVKLGKHEICNKEMASKC"
BASE COUNT         1449 a          768 c         1012 g         1173 t
ORIGIN      
        1 aggggatatt agcattagaa gtgcccggga tgttcccaca atcatatatg aaagccgttg
       61 atatatatct tttcatgctc attttttatt aatgaacctt caataaattc attaatgata
      121 atttcttcaa taatctttga cattgcagat ttatcatact gatgataata actattcgta
      181 tttatacgaa ttagttttat ttagatatgt ttatatatcc gtttgaacat tcttaagatt
      241 gccaatttta gccttttaga agagggctga ttgagtaagc aacaacatga tattatgaga
      301 aaatcaagca tatgtaatca aatagctgat acgaatagaa caaagaacac ttttgcaacc
      361 ccggaactcg tcgggcgcag ggtggctttc cagatactta ttaatggaaa aaaaggcgga
      421 cgggcaagac cgtatttaat gtatgatata ggaacggtca aggaagtccg aaactctgcg
      481 gtgatcatag aggggaacag gggacaactc tacaacaggc ctgttgataa gatcactctc
      541 ctggaagcgg ggaggtgaga tcttgatcct gcaaacaagt gaagagcgaa tcaaactcct
      601 caaatcagga ttcaccgggg atcaggtcga agcgctgtat attgatctga atcattttga
      661 aacggtggga gtgaattggg gcgattttgc catgaaccgc agtagctctc atatatttgc
      721 caggaaaact cgcttatctc gtcgcgggcc tgttttgcag cgacatctat ctattttgta
      781 ttatggacac tgggagcatt gcacatgatc ctactgcaga cctggaacgg tgctgccatg
      841 aaacgcaccc gaaggattct aaccacactc cttccttcaa tgaatctgat ttttctacga
      901 tccataaccg cagattctgt gtgcatgcaa gcagctccgt ccctggggat gagagcatga
      961 atgaagataa atggacgaaa agaagaaaag atctatgtaa tgagttttgg gacgattgcg
     1021 ctcaagaatc atgtgatact ccttgttcaa gaatccttga aactgaaaag agggggccat
     1081 gacctgcgga cagtatcctg gagagggaca taaagatcat agttgtgttg tatgcacaaa
     1141 tctgtttaat ggctgcaaac tgctatgcag tccgagcaaa aaatcctgcg atttcaaact
     1201 agcaactccg gaaattctga aaagacgtgt tgagcttttt agcagtaaga ggaagatatg
     1261 atatccaggg agaagattga aaaacggata ctggaggaat atgaaaattt cgtattccag
     1321 ggatattcaa ccgaagagat ctcttatatg gggacatgcc tgcaaaagtg ggcaattgag
     1381 aaaagaatag gtcccgcaaa tatcgagcca ttaagccaga taaaaccgac ccccgactat
     1441 tggttgaagc cactgattgt gcctgtgaac acaaacactt tgacagccat ggcagttcat
     1501 ggcgccttgt gcctggcgct gcgccatcct caattcaatg gtgatggaaa acatgggcat
     1561 gaattaagtc gaaaaatggt ggttgaattc acaaagtccc tgggtgaatg gttagttcat
     1621 gccggtgccc tgacacctga gcagctccga tccgttgagg aactcgaatc caaggaagga
     1681 tcttcagact taattcatga agccaagcca ggagtgcaat gatgcgattt actattgaag
     1741 ggaccaatgg ggaaatcaag gagttcataa gcgatatagc gcaaaaaccg cgggatcttg
     1801 tccttccgga aggacctatt tccgaacatg gatatggaga aaaaattttt caaaggcttg
     1861 aggaaaagct cagtaaaaaa gattctgtcc ctatttcagc tcaggcctca gataaggacc
     1921 tggacagaat agaaccggat catcatcttg acgtatatgg gggagtggat tgatggccga
     1981 taatgcaatc atcgttattg acggtatccg tatcctctcc gatctggaat ctgaatatga
     2041 aagaaagcat tctgaatgga agatccagtg ttcagctgtg catgctcgat tcaagaaaaa
     2101 gacatccagg acgaaaaaga aaggtaagga atatgaatac acgatctggt atgaagagca
     2161 gcccggggac ggcctaaagt ctatcgggaa ggaagaaccc gattatagta aatactatcc
     2221 tccagagcct aagccatcgg gttttatcga gttccaggaa tatgtccagg aatatgaagg
     2281 ccatttactt cttacagaga ggtattatca aaattataaa acgattttta aggactgcct
     2341 tgttttcagg cttgcggagt gtttgaataa taaccatccg ctaatcggga ggtgaatcat
     2401 actgcagtta gaattgggta tcattccgct ttctgtggag attaaagagg ggatgaaggt
     2461 ggcgttcttt gttagtatca aaaggcatag agcaaggaag aaagaggttc atattggtga
     2521 ggttaggtcc ttcaatggca ttggcgtaat aatttccggg attacaatgc ctggaatata
     2581 taatcgcgct cctgacaagc tttttgaagt gcataaagat ctgagcctgg gggcgtttaa
     2641 atgaatcgtc ttatcttcaa agaccgacgc ggtgatgaat atgtattcat tcgatatagt
     2701 acgaatgaaa ttaaggcgac gaaaaaatcc cagttgagtg aaggatacca tcaaattgat
     2761 tttagtgctg aagctattga aaaattgcat gaacttttac atcaggagga aagataattt
     2821 ccgaaccatt tattcacaga tatcattctg gaatgttctg cccgataatc aagatgatgt
     2881 gccgatctga ttgtgtcgct ttaagatctc cggatttttg tatattgata gatggtagtt
     2941 tcagtctaag caggcggatg gaggattttc tagcagagtt tgctttcgaa agactagagc
     3001 ttaaggaggc acataaatga gcgagtgtct agggaagttt gtaggtacac agcgctgtat
     3061 ccgtcagacg ctgtatccag gcatgtgcac gaacaaatgt ctggggacgt ttgctgttat
     3121 ccagcgctgt atccgtcccg ctgtatccag acgaatacaa gagcaaaaga cattgaagtt
     3181 ttcatatagg tttaaatttt ctattttaag actttctatt tatccgattt tttcttgcgc
     3241 tgtatccagc gccgtatccg tctttattag acagaaaacc ataaacagaa accgcgtttt
     3301 ttcgaaattc caacattcca cttatcagtt tatcccttca gctgtatcca gcgctgtatc
     3361 cagtctaaaa aattgtgtga agagctgtat ccagtctttt gagggaatga acgatgagaa
     3421 aaatttagta tataaaggag gtggttttga agtacctgta atagtgtaaa cgttataaag
     3481 tttattataa cgttacataa ccattagttt acggtgtaat aaaatccgtt tgtaaaatcc
     3541 gtttgtgtca cactgtatcc gtaaaaagct ggatacagcg tttgacggat acaagacgga
     3601 tacagcgtgg gacctgatac aggtcaggac ggatacagcg aaaccaaaag agaatatgaa
     3661 taaaaaagaa agagaccaaa tatatgaatt aaaatatcgt aaggcgagga tagataagca
     3721 cgatactcca gtgaataagt ttcttgataa agccatagaa gaaaaaaaaa gtgtcaccag
     3781 ggccattcat gaattattag atcgcgagac tttatcccaa gcacaattaa gaagaaatga
     3841 acaggataag atggaggaag ctttaaaatt gtgctcagta atattcaaaa atatattgtc
     3901 catttataga gctcaacagc ttgaattgtg gctcataact ttcaaaagag tattcatgat
     3961 acttgataag gcagccgccg aaggtgcaca ggccagcaaa gaagacgata aagaatatct
     4021 tcaaagagca gtcgaagcga taaaagaaat ggaaagggat aacacagaaa agcatttaca
     4081 aaatgcaatt ccaataaata gattgaataa tgcatataaa aaataaaaag caaatgacag
     4141 agttatatga gttgattaaa aatgggtaga aaaagaaagg agagtaagcc acctgaactc
     4201 tatgtttgcg tggattgtgg taaacaagct gaaaaaggat atatgaaact tgtctccaga
     4261 aaaatagaac tgtatccgtg tggattgccg caaatcgatg gaaatatact ttacaccact
     4321 gaagaaatgg atcatgccca gaagatttgt gtgaaattag ggaagcatga aatatgtaat
     4381 aaagaaatgg ctagtaagtg tt
//
LOCUS       DATGBN010000426         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_228555, whole genome shotgun sequence.
ACCESSION   DATGBN010000426 DATGBN010000000
VERSION     DATGBN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228555"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VKL21_10750"
     CDS             complement(<1..950)
                     /locus_tag="VKL21_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46290.1"
                     /translation="MVKMSAVKKEEVEEKKDIFRTWTDSYIEVSKIWENSYEKLYKPW
                     LESSGEMFEKTALLSKEATPQKYREFYDEWINTYQNTFGKFHKIPTPESNKEILEKFL
                     LSADESNKLYRSWIAELEENSQKTREILQGKPDPVKYKECYDIWTKSYEKIFEGIMKL
                     PSQESIKELFGDCIGIPDSYMEGFLQMSKLWTKSYTQICRPMNESMLKLSEKMAEISR
                     GDAGPESYKEFYLLWMESYNEIYGKYIASMEPSREMFENFAQSTNIYLSIYKSWITAL
                     EKMSEKAQELSKHNADLEAYKGFYDLWINTYEKAFESFFE"
     gene            complement(1115..1428)
                     /gene="ffs"
                     /locus_tag="VKL21_10755"
     ncRNA           complement(1115..1428)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VKL21_10755"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            1506..1739
                     /locus_tag="VKL21_10760"
     CDS             1506..1739
                     /locus_tag="VKL21_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMB46291.1"
                     /translation="MEELIGYVASNHKASKILNVLDSKGAMDGATIAKTVRIVGADKI
                     IEELVGKNLVVREGAKFALTELGKQVSHKLRSV"
     gene            1774..2265
                     /gene="cgi121"
                     /locus_tag="VKL21_10765"
     CDS             1774..2265
                     /gene="cgi121"
                     /locus_tag="VKL21_10765"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HMB46292.1"
                     /translation="MISILEGEIFIEDIEMFLRKIKEKRKSKDTVILALDADKLAGEK
                     HLKFAIEKAMNSFKTGRNIAKDPGKEIMLYAAGTRQINRAMKIGVHNGKNNIALVGIG
                     NDIDLSVFDEITSGNVLQYDQSKNRVLMDFFNITDEEIKAVGVDKVPELVLERVALVD
                     VMK"
     gene            complement(2292..>2718)
                     /locus_tag="VKL21_10770"
     CDS             complement(2292..>2718)
                     /locus_tag="VKL21_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HMB46293.1"
                     /translation="DMLKSGKHPWKNLKDQILAEKDPAKQMELRKKSITSEYFLGSVH
                     AVAETGEILIVNATGSSIPSCAFSSDNVIWIVGTQKIVPTLEEGFKRIREHCVPLEDQ
                     RMKSVGYPGTTIGKYLIFEREINPNRKINLIFVNEKLGF"
BASE COUNT          762 a          551 c          552 g          853 t
ORIGIN      
        1 tcctcaaaaa agctctcaaa agccttttcg tatgtattta tccagagatc ataaaatcct
       61 ttatatgctt ccagatcagc gttatgcttg gacaactcct gcgctttttc tgacattttt
      121 tcaagtgctg ttatccagga cttataaatg ctcagataaa tgtttgtgct ctgtgcaaaa
      181 ttctcaaaca tttccctgga aggttccatg gatgcgatat atttaccata tatctcatta
      241 tatgattcca tccataacaa ataaaattcc ttataggatt caggacctgc atctccccgg
      301 gagatttcag ccattttctc agaaagtttc agcatggatt cattcattgg cctgcaaatc
      361 tgtgtatatg atttcgtcca tagttttgac atctgtaaaa agccttccat gtagctatcc
      421 ggtatgccga tgcaatcccc aaataattct tttatacttt cttgagaagg cagtttcatg
      481 ataccctcaa atattttttc atatgatttt gtccatatgt catagcattc tttatatttt
      541 acaggatccg gtttgccctg gagaatttct cttgtctttt gggaattctc ttctaactcg
      601 gcaatccacg acctgtagag tttatttgat tcatcagcgc ttaataggaa tttttcaagt
      661 atctctttat tggattctgg cgtcgggatc ttatggaatt tgccaaaggt attctggtat
      721 gtattgatcc attcatcata gaattccctg tatttctgcg gcgtggcttc ttttgaaaga
      781 agagccgttt tctcaaacat ctctccactg gattcaagcc agggcttgta caacttttca
      841 taggaattct cccaaatttt tgagacttct atataactgt cagtccatgt cctgaaaata
      901 tccttctttt cttcgacctc ttctttctta acagcactca ttttgaccat tatttattcc
      961 tccgagtcca ttataatgtt tttccctatt gggtgggaat atttacaaat tcactcccta
     1021 taataattac gttgttatgc tatataaaga tttttttttc attattattt atagagaaaa
     1081 atcgcaggta catctgcgca aacctaataa taagtagcag gcatgccttg ggtcgcagcc
     1141 tcttccactg cacggttggc cacgggctcg cctccgcccc gcgcccccat gagcgatggt
     1201 tgttcacggc tggtttgctc ccttccgggc ctagaccttt ttccgcaact aaggtacaaa
     1261 aaccttcctt caggaaagcc tctgtaccaa caccagcccc ataggacggg gtttcacagt
     1321 agaacctgtg ggccatgcaa tagtcaaaac ttcttccttc ggcttcgccc cccgctatcg
     1381 acggtttcgg gttatagata acgccgagtt acccgggcta gcccgccgcc tttgttatgt
     1441 tcctcgaatg caataaagtt atctcaaaag aggtacaata taggagaaaa ggagatgaaa
     1501 aatttatgga agaattgatc ggttatgtcg caagtaatca taaggcgagc aagatactga
     1561 atgtacttga ttcaaaagga gccatggacg gagcaacgat agcaaaaact gtgaggatag
     1621 tgggggcgga taaaataata gaagaattgg tcggcaagaa ccttgtcgta cgtgaaggtg
     1681 cgaaatttgc tttgacagaa ctggggaagc aggtatctca taaacttaga agcgtatgat
     1741 acgggtattg ctttttggga atgggaaaat tatatgatca gtatcctgga aggtgagata
     1801 ttcattgaag atattgaaat gttcctcagg aaaataaaag aaaaaaggaa aagcaaggac
     1861 actgtgatcc tggcgcttga tgctgacaaa ctggccgggg aaaaacatct caagttcgct
     1921 atcgaaaaag caatgaattc atttaaaacg ggcaggaata tcgccaaaga ccccggaaaa
     1981 gaaataatgc tttatgcagc aggcacgcgc cagataaaca gggcaatgaa gatcggtgtt
     2041 cataatggaa agaacaatat tgcccttgtt ggtatcggga acgatattga tctgtctgtt
     2101 tttgatgaga taacttcagg gaatgtgctc caatatgacc aatcaaagaa ccgggtttta
     2161 atggattttt tcaatataac cgatgaagag atcaaagccg tgggtgtgga taaggttcct
     2221 gaacttgttc ttgaaagagt ggcgcttgtg gatgttatga agtagaagaa aaaaacataa
     2281 atgaaaatgg atcagaaacc cagcttttca tttacaaaaa tgagatttat ttttctgttt
     2341 gggtttattt ccctttcaaa tatcagatat ttcccaattg tcgtacctgg ataacccaca
     2401 cttttcattc gttgatcttc cagcggaaca cagtgctcac gaatgcgttt gaatccttct
     2461 tccagtgttg gaacgatttt ttgggtccct acgatccaga taacattatc cgaggagaaa
     2521 gcgcatgaag gaatcgagct tcctgttgca tttactatga gtatttctcc tgtttctgca
     2581 acagcatgaa cacttccgag gaagtattcg gatgtaatgc ttttcttacg aagttccatt
     2641 tgttttgcag gatctttttc cgcaaggatc tggtctttca ggtttttcca gggatgtttc
     2701 ccggatttaa gcatatct
//
LOCUS       DATGBN010000427         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_228600, whole genome shotgun sequence.
ACCESSION   DATGBN010000427 DATGBN010000000
VERSION     DATGBN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228600"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VKL21_10775"
     CDS             <1..464
                     /locus_tag="VKL21_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMB46294.1"
                     /translation="YRTIENVIEGAVVTFMDITGRKRAEQLEHDARIFTEGIIDAVRE
                     SLLVLDKDMYVISANRSFYRTFKTSKEETENKIIFDISNRSWNIPALRKLLEEILPEN
                     VMFNDFEVEHDFPEIGYKKMLLNGRRIYQESMGADRILLAIEDITGQNCKG"
     gene            complement(620..1126)
                     /locus_tag="VKL21_10780"
     CDS             complement(620..1126)
                     /locus_tag="VKL21_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HMB46295.1"
                     /translation="MLSHDELISLIDEKPHLVEKMIDPDIQVQPNGIELTLQRVEFHD
                     GTGSIAFDNSERKLPPTKNLDFDDNGWLDLPKGSYKIVFNEIVNIPNNIAAIAKPRSS
                     LLRCGVTVETAVWDAGYSGRSESLLVVYNENGFRIKKDARVLQLLFFRLAKEVRKGYS
                     GVYQNENV"
     gene            1215..1379
                     /locus_tag="VKL21_10785"
     CDS             1215..1379
                     /locus_tag="VKL21_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46296.1"
                     /translation="MNRTITIAKDVDWIVKKYRVDLINITRKGKARLQRYNTIRLATA
                     ENVAKYSACL"
     gene            1468..2292
                     /locus_tag="VKL21_10790"
     CDS             1468..2292
                     /locus_tag="VKL21_10790"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HMB46297.1"
                     /translation="MKQEEIKKMVRERYASVAREGGSCFSPASSCCTSATLAEDISRR
                     IGYGDEELTCVPEGANLGLGCGNPLALASLKEGEIVLDLGSGAGFDCFLAAKRVGKEG
                     KVIGVDMTPDMIDRARNNARKGNYANVEFRLGEIENIPAADNSVDIVISNCVINLAPD
                     KIGVFKEAFRVLKPGGRLMVSDIVLLKELPDFVRDSIEAYTGCVAGATKKDEYLNTIK
                     NVGFKEVQVLDETNYPLDFLENDPIGKQVVEKLNMPIENMKEIGKSVVSIKVQAKK"
     gene            2403..>2878
                     /locus_tag="VKL21_10795"
     CDS             2403..>2878
                     /locus_tag="VKL21_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46298.1"
                     /translation="MINRAITNRHPMKKTSPGCAVIVFAKFVRISGATWLAAPLSSAK
                     GCIGIGVDVVLALHSPTDVGAEVGTTPAISLPFMVSTIPKGVDVMAGTPEMAGGGREY
                     DSLFLRPSPRLKSGDSRVHITSLWLSSSLIRITASTASVIVASGLTGDPLSLRPTP"
BASE COUNT          888 a          571 c          697 g          722 t
ORIGIN      
        1 cctatcgcac tatagagaat gttattgagg gcgctgtggt tacatttatg gacatcaccg
       61 gacgaaagcg ggctgagcag ctggagcatg atgcaaggat atttacggaa ggcatcatag
      121 acgcggtgcg ggaatccctc cttgtactgg acaaagatat gtatgtaata tcggcgaacc
      181 ggtctttcta caggacattc aagacttcta aagaagaaac tgaaaacaaa ataatcttcg
      241 atataagcaa tcgttcgtgg aatattccag cgcttcgaaa gctgctggaa gagatcctgc
      301 ctgagaatgt catgttcaat gacttcgagg ttgagcatga ttttccagaa attggctata
      361 agaaaatgct gctcaatggc cgcaggatat accaggaaag tatgggtgca gacaggatac
      421 tgcttgcgat tgaagacatt accgggcaaa actgcaaggg atgatatcaa tgttatgtag
      481 tgaactatac acaaataatg agagtgaata aaagaagata aaccaaacac tgaaaaaaga
      541 acccacattc acaagcaagc ggaagaaatg tcaaagggaa aaaccacata cctggacctt
      601 attccaggaa gagatctttt caaacattct cattctgata tacgccggaa taacctttcc
      661 tgacttcttt cgcaagcctg aaaaacaaaa gctgcagcac acgcgcatct ttttttatcc
      721 tgaatccatt ctcattatat accacaagga gactctcgct cctcccgctg taaccagcgt
      781 cccagacagc cgtctccacc gtcactccgc aacgcaggag gcttgagcgc ggctttgcta
      841 tcgcagcgat attgttgggg atgttaacga tttcattgaa tacgatctta taacttcctt
      901 ttggcagatc cagccatccg ttatcatcaa aatcaaggtt ttttgtgggg gggagttttc
      961 gttcggaatt atcaaaagct attgatcctg tcccgtcatg aaattccaca cgctgcaggg
     1021 tcaattcgat tccatttggc tgtacctgga tatccgggtc gatcattttt tcgacaagat
     1081 gcggcttctc atcaatgagt gagatcaatt cgtcatggga taacataggt tcagaattag
     1141 tcgaggattt gataaacatt gctgaacccg tgaagctaaa aatcctggtt aagaatcatt
     1201 gaggatttca aagaatgaac agaactatta ctattgcgaa ggatgttgac tggattgtta
     1261 aaaaatacag ggtggatctg attaatatca caagaaaagg aaaagccagg ctgcagcgat
     1321 ataatacgat tcgtcttgca acggctgaaa atgtggcgaa gtattctgca tgtctttgat
     1381 caaggttgag aagctcgcag agattgtaaa aggtatgctt ggccccgtga tgaaggatat
     1441 aatggaaaaa taaaaggagg tataagaatg aaacaagaag aaataaagaa aatggtgaga
     1501 gaacgttacg caagcgtagc cagggaaggc ggatcctgct tctctcctgc aagttcatgt
     1561 tgcacaagcg ccacacttgc tgaggatatc agcaggagaa taggatatgg cgatgaagaa
     1621 ctaacttgcg tacctgaagg cgcaaatctt ggtcttgggt gcgggaaccc attagcgctt
     1681 gcttcgttaa aagaagggga aattgtgctc gatcttggct caggcgccgg atttgactgc
     1741 ttccttgcag ccaaaagagt tgggaaagaa ggaaaagtca taggtgtgga tatgacgcct
     1801 gatatgattg atcgggcaag gaacaatgca aggaaaggga attatgcaaa tgttgagttc
     1861 aggctcggtg aaattgagaa tatacccgcg gccgataatt ccgttgatat tgtcatttca
     1921 aactgtgtca tcaacctcgc cccagataaa atcggggttt ttaaagaagc cttcagggta
     1981 ctcaaaccag gggggagatt aatggtttca gacattgttc tcctgaaaga acttcccgat
     2041 tttgtcaggg attccattga agcatatacc ggatgcgttg ccggtgcaac aaagaaggat
     2101 gaatacttaa atacaataaa gaacgtcggc ttcaaagaag tgcaggtact tgatgaaaca
     2161 aattatcctt tggatttcct ggaaaatgat ccgattggaa aacaggtcgt tgagaaactc
     2221 aatatgccaa tagagaatat gaaagagata gggaaatcag ttgtcagcat caaagtacag
     2281 gcaaaaaaat agaaaggtgg aattccggga acaaaaagga aataaggatg tgctattaca
     2341 ggatcgacag gagtggattg aaaatttatc ctgttatttt cctttttctg aagtaatcca
     2401 ttatgataaa cagagcgatc acaaacaggc atcccatgaa gaaaaccagt ccaggatgcg
     2461 cggtaatagt gttcgctaag tttgtgagga tatcaggcgc cacctggctt gccgcaccgt
     2521 tatccagcgc taaaggctgc ataggaatag gcgttgacgt ggtattagcg ctccattcgc
     2581 caactgatgt tggcgcagaa gtgggaacta cgccagcaat ctctttgccc tttatggttt
     2641 caacaatccc gaaaggagtg gatgtaatgg caggtacccc cgaaatggca gggggaggca
     2701 gagaatatga ctcgctgttt ttgagaccct ctccacgact gaagtcggga gattccagag
     2761 ttcatataac ctctctgtgg ctctcatctt cattgattcg gattacagca tccacagcct
     2821 cagttatagt cgcttctggt ttaacgggcg atccgctcag tctccggcct actccgaa
//
LOCUS       DATGBN010000428         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_73113, whole genome shotgun sequence.
ACCESSION   DATGBN010000428 DATGBN010000000
VERSION     DATGBN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73113"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..188
                     /locus_tag="VKL21_10800"
     CDS             60..188
                     /locus_tag="VKL21_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB46299.1"
                     /translation="MSAKIISICNQKGGVGKTTTAVNLSAGLLHVGKGVLLINMDS"
     gene            complement(357..533)
                     /locus_tag="VKL21_10805"
     CDS             complement(357..533)
                     /locus_tag="VKL21_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46300.1"
                     /translation="MIFMCSTYVPFEIKPEELATKGREYGMFWCISCKKTFLKKGATE
                     CPICGSTKIKPRAP"
     gene            680..1048
                     /locus_tag="VKL21_10810"
     CDS             680..1048
                     /locus_tag="VKL21_10810"
                     /inference="COORDINATES: protein motif:HMM:NF040210.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5830 family protein"
                     /protein_id="HMB46301.1"
                     /translation="MSSKIDAGLELISALGAIELKPIEIRDLLYNLISKDYNTIDKIL
                     ETAQKEKLLKLTEKIYLITPEASNLQFEKPKIVKQEESATCRFCRKKLSTGYYLVFKS
                     HTYGPYGSSCIRKMYLDYLL"
     gene            1296..1604
                     /locus_tag="VKL21_10815"
     CDS             1296..1604
                     /locus_tag="VKL21_10815"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMB46302.1"
                     /translation="MESVQIRIDEKNMKNLNELAQMLRSSRSEVIRRIIDGGIRELRM
                     KIAMEKYIDEEFSLCRAAEFSSVSIQQMARYLTQRGIPFFKQSIEESEKDVIEAQTWL
                     "
BASE COUNT          545 a          351 c          294 g          474 t
ORIGIN      
        1 tccttcaacc gataaaaatt taaaccagtt aatcatttaa ccacacatcg gagggcaaaa
       61 tgtcagctaa aataatcagt atatgcaacc agaaaggcgg tgttgggaaa accacaaccg
      121 cagtcaattt atcagccgga cttctccatg tcggaaaagg ggtgttactc atcaacatgg
      181 actcttaacc ttttttattg agcgcaaatc tccatcggaa catgcccagc ttcccaagga
      241 aaaatagggc ctacactctc tccttccccc gtaccccaac ctctcaccta gctgcatgca
      301 tcttaagatc cgtattttcc aagataagtg acttaagtta ttcacatgcc caagttttac
      361 ggcgccctcg gctttatttt tgtagagccg cagatcgggc attcagttgc acctttcttc
      421 aagaaggtct ttttgcatga aatgcaccag aacattccat attcccttcc cttagttgca
      481 agttcttccg gttttatttc aaatggcaca tatgtgctgc acatgaaaat cattgctatt
      541 tctacctcca atataatgtt ttatactcac taagtctgca aggtcagaaa gaagatgcat
      601 gcaatgcata tcgatcaaac tttggcagac aaacaattta tatttcgctg atgaagtaga
      661 tatagcaggc aggtaagaat tgtcctcaaa aatagatgcc ggacttgaat taatttccgc
      721 actcggcgcc attgaattaa aaccgattga aataagggat ctgctttaca atttgatttc
      781 gaaagactac aacacaatcg ataaaatcct ggagactgcc caaaaagaga aacttttgaa
      841 actaactgaa aaaatctatt taataactcc ggaagcatca aatcttcagt ttgaaaaacc
      901 taaaatcgtt aagcaggagg aaagtgccac ctgtagattt tgtagaaaga aactatccac
      961 agggtattac ttagtattca aatctcatac ctatgggcct tatggatcat cctgcattcg
     1021 gaaaatgtac cttgattatt tattgtgaaa agaaatccat gctattggaa aatctttgca
     1081 ttcaaaatat atcctcgtct tcttcattga catcatcccc ctccttatat tcagttttct
     1141 ctctcccttt tccacacctc aaccctctta ctgagctgga acgaatctgg ttatgaaaca
     1201 tcaacgtata cgtttacgtc cactcaaggg ggtggaatgg aatttctaca tactacaacg
     1261 caagaattaa ttacatttaa ttacaatgta ttatcatgga aagcgtacag ataaggatcg
     1321 atgaaaaaaa tatgaagaat ttgaatgaac ttgcacaaat gctccgttca tccagatcgg
     1381 aggttatacg aaggataata gacgggggta tcagagagtt acgtatgaaa attgccatgg
     1441 agaaatatat tgatgaagaa ttttctttat gcagagcagc agaattttcc agtgtatcta
     1501 tacagcagat ggcaagatat cttacccaaa gaggtattcc cttttttaag caaagtatag
     1561 aagagagcga aaaggatgta atagaggccc aaacctggct ataaaatgcg cgtactcatt
     1621 gattcatcta ctcttatcgc tctggcaaaa ataggcaaac tcaa
//
LOCUS       DATGBN010000429         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_306198, whole genome shotgun sequence.
ACCESSION   DATGBN010000429 DATGBN010000000
VERSION     DATGBN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306198"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VKL21_10820"
     CDS             complement(<1..419)
                     /locus_tag="VKL21_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HMB46303.1"
                     /translation="MKDVIETIKTRRCVREFKDEAISDEDIKFLIDCARYAPSGFNMQ
                     PWSFLVIRNKDSLLKLSESGKKAMIPILEPMKNAGKKASDFLVFLKTKGTSLFYNAPV
                     LVIIFGNKNAPTVDFDCAMAAQNMMLAAHSKGIGSCW"
     gene            complement(481..702)
                     /locus_tag="VKL21_10825"
     CDS             complement(481..702)
                     /locus_tag="VKL21_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46304.1"
                     /translation="MAITFTDEELMYIDKDSDDKLRSIRQTIDTLKSKGDIAFCKKCG
                     HMMQYFTVGNAKLLQCVFCGNRVAEMIQL"
     gene            773..1459
                     /locus_tag="VKL21_10830"
     CDS             773..1459
                     /locus_tag="VKL21_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HMB46305.1"
                     /translation="MYIITLQAIALYLISQYLHRSLSFASRTLYIVLMLPGTIAHETC
                     HAVVALLMGARITKFSIIPSGDTLGHVEYTSPKIPLIGNVAISIAPLIGCPAILLLIS
                     GYFGVHFDFPPASFDIFAGSRFLLDGTLSFITGLDYLNWKTYVFLYLALTLGAGAAPS
                     KTDIISMLPGLIVIVAAVYSLNYFGVNIQYLYNVFSWLSTALSVAMIPLLVVTIIVGM
                     LKSIAILRRT"
     gene            complement(1854..2117)
                     /locus_tag="VKL21_10835"
     CDS             complement(1854..2117)
                     /locus_tag="VKL21_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46306.1"
                     /translation="MIRIIDQFLQELKINGTAEKTITDYSKFLKNINRLKSLEKWEKT
                     DVNKYILEKHNECLTETVKVLKVRLKRFFTWAGKSELVNHLNT"
     gene            complement(2237..2320)
                     /locus_tag="VKL21_10840"
     tRNA            complement(2237..2320)
                     /locus_tag="VKL21_10840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2285..2287),aa:Ser,seq:gga)
     gene            complement(2349..>3280)
                     /locus_tag="VKL21_10845"
     CDS             complement(2349..>3280)
                     /locus_tag="VKL21_10845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HMB46307.1"
                     /translation="MYGKKPHDRPIEEYIRKGAINLDKPAGPTSHEVTSWVKKVLDLK
                     KAGHSGTLDPNVTGLLPIMLEDATKAVDGLLLAGKEYVCLMKLHTVMPENVIKNVLSE
                     FTGDIFQKPSIKSAVKRVTRVRTIYYNELLEIEDNNVLFKVGCEAGTYIRKLCHDMGM
                     AMGTGAHMQELRRTKSGPFREDGTLITLQDLRDAYVEWKENGNESILRKVILPMEFGV
                     SHLPRIVIRDNAVDAVCHGAGLAAPGVLSVETGIEKGEFVAVFTLKGEIVSLGDAQMK
                     SADILKAATGIVVTTDRVLMDAGTYPKGWKAKE"
BASE COUNT          864 a          752 c          566 g         1098 t
ORIGIN      
        1 atccagcagc ttccgatacc ctttgaatgc gctgcaagca tcatattctg tgctgccatt
       61 gcacaatcga aatccactgt tggtgcattc ttattcccaa atattattac aagcaccgga
      121 gcgttgtaga aaaggcttgt cccctttgtt ttgagaaaaa caaggaaatc agaagctttc
      181 ttgccagcat ttttcatggg ttcaagtata gggatcatcg ctttctttcc gctttccgat
      241 agcttcaaaa gcgagtcttt gttcctgatc actaaaaagc tccatggctg catattaaat
      301 cctgatggtg catatcttgc acagtcaatc aggaacttta tatcttcatc tgatattgct
      361 tcgtccttaa attcacggac acaccgcctt gttttgatgg tttcaattac gtctttcaaa
      421 ataacacacc tattttttaa gggttttgat ttcagttata gttttccctt aaatcagaac
      481 tcataattgt atcatttcag cgactctatt cccgcaaaaa acacattgca gaagctttgc
      541 atttccaact gtgaaatact gcatcatgtg gccgcacttt ttacaaaatg ctatgtcgcc
      601 tttcgatttt aaagtgtcaa ttgtctgtct tatagatcgc aatttatcat ccgaatcttt
      661 atcaatatac atcagttctt catcagtgaa tgttattgcc attgtatttt atatccttcc
      721 agttcattct attattcata tattcaatac atatgcttac cgcaaattta acatgtacat
      781 cataacactc caggcaattg ccctctacct gatctcacaa tacctgcatc gttccctgtc
      841 cttcgcatca agaacccttt acattgttct tatgctccct ggaacgatag cgcatgaaac
      901 gtgtcatgca gtagttgctt tgctcatggg cgcgcgcata acaaaattca gcatcatccc
      961 ttcaggagac actttaggac atgtagaata tacatctcca aagatacctc tcattggaaa
     1021 tgtggccata tcgatcgctc cattgatagg atgtccggca atactgctgc ttataagcgg
     1081 ctacttcgga gtacattttg attttccacc agcctccttt gatatattcg caggatcaag
     1141 atttttactt gatggaacac tttctttcat caccggcctg gattatttga actggaaaac
     1201 atatgttttt ctttatcttg cacttacgtt gggagcaggt gcagcgccca gtaaaaccga
     1261 tatcattagc atgcttcccg gtctaattgt tattgttgcg gcggtatatt cattgaacta
     1321 ctttggtgtc aatattcaat atctgtataa tgtattctcc tggttaagta cagctctttc
     1381 ggtagcgatg atccctttac tcgttgtaac aatcattgtt ggtatgctaa aatcgatagc
     1441 gatcttaaga cggacatgaa attttgttta taacgaaaaa gatactttag aaaaggactt
     1501 attgaaattt atcgctggca gacttgcatg ttttgatgtg tgtgcagtta gcaacccggc
     1561 acactttgat ttttgcacca cattgattac agtaaaatcc tgcggaaaat attaaaggtt
     1621 gggtattggg gatagtcatg taagccatca attcgcctcc ttgttttgtt tatttattcc
     1681 tgttttcata gcttctatta tatgatcttc atctgaatcc atcatcttca acaatatcct
     1741 ccttgctttt atatctattc tcctaacttc tcacaggctc aatttaatca tgttattcga
     1801 ggggtttacc cgttagttca aggaagagag gtgtcccctt gcaaaaggtg gaattacgtg
     1861 ttaagatggt ttacgagttc ggatttgccc gcccatgtaa aaaaccgctt tagcctgact
     1921 tttaaaactt taactgtttc agtcaaacat tcattatgct tttccagaat gtacttattc
     1981 acatcagtct tttcccactt ttcgagtgac ttaagcctat tgatgttctt caaaaatttg
     2041 ctataatccg ttatagtttt ctctgccgtg ccattaattt taagttcctg aagaaattga
     2101 tcaattattc taatcatatt ccataatatg taattattag gtaataaagc cctataagaa
     2161 cttttcaaaa tctttggtat taataccatc ctgagcaaat ttcagcttgt ttttgagccg
     2221 tcctgaataa tcattacgcc gaggggggga ttcgaactcc cgtggggcag agccccacaa
     2281 gctttccagg cttgcgcctt agccgctaga ctacctcggc acgaaacact aaataaacga
     2341 tatgattctt attcttttgc tttccatcct ttcggatatg tgcccgcatc cattagaacc
     2401 ctgtcggtag ttacaacgat cccggtggct gctttcaata tatctgcgga cttcatttgc
     2461 gcatcgccta aggatactat ctcacctttt aatgtaaaaa ccgctacaaa ttcacctttt
     2521 tctatcccag tctcaaccga aagcacaccc ggggctgcaa gacctgcgcc atggcagact
     2581 gcatccaccg cgttatcgcg tatcactatt cttggcagat gcgatacacc gaattccatc
     2641 ggaagtatta cttttcgcaa aatgctttca tttccgtttt ccttccattc aacataagca
     2701 tcccgcaggt cctgaagagt gatcagggtt ccatcctcac ggaacggccc tgattttgtg
     2761 cgccgcagtt cctgcatatg ggcgccggtc cccattgcca ttcccatgtc gtgacataat
     2821 ttacgaatgt aagttcccgc ttcacatccc actttaaaga gtacattatt atcctctatc
     2881 tcaagaagtt cattataata aatggtcctt actcttgtaa cacgctttac tgcagattta
     2941 atagaaggtt tctgaaatat atcgcctgta aactcggaaa gaacgttctt tattacattt
     3001 tccggcatta ccgtatggag tttcatcagg caaacatact cttttcctgc cagaagaaga
     3061 ccatcaactg cttttgttgc atcctcaagc atgataggaa gaaggcctgt tacattaggg
     3121 tcaagcgtcc cactatgccc tgcttttttc aaatcaagca ctttttttac ccatgaagtc
     3181 acttcatgac tggtcggacc tgcaggttta tcaagattta tcgctccttt tctaatatat
     3241 tcttcaatcg ggcgatcatg aggtttcttt ccgtacataa
//
LOCUS       DATGBN010000430         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_189897, whole genome shotgun sequence.
ACCESSION   DATGBN010000430 DATGBN010000000
VERSION     DATGBN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189897"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..389
                     /locus_tag="VKL21_10850"
     CDS             144..389
                     /locus_tag="VKL21_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46308.1"
                     /translation="MCAKVSYLSDKYTLSQIMDGYREAVKLLYIHIFEYMTEKGFFGH
                     LSGEIPLNDLMEKMNISKDRKQIFLAISANLRYLISI"
     gene            389..739
                     /locus_tag="VKL21_10855"
     CDS             389..739
                     /locus_tag="VKL21_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMB46309.1"
                     /translation="MTFINSDVDDGLPFLESGKYDGVMAIGLLHFLRKPEQFLSEVSR
                     ILKPGGKFAMGNQYLIKPLMYQGATDLYFSIIGQYTRAHTPDQIRQMLNSSGLNPNYT
                     FEIGNYGWFYARKE"
     gene            805..1562
                     /locus_tag="VKL21_10860"
                     /pseudo
     CDS             805..1562
                     /locus_tag="VKL21_10860"
                     /inference="COORDINATES: protein motif:HMM:NF021398.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
     gene            complement(2065..2808)
                     /locus_tag="VKL21_10865"
     CDS             complement(2065..2808)
                     /locus_tag="VKL21_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMB46310.1"
                     /translation="MTAHPSGRKLGIHLILPPDLKKCKGLKVLGISGSSRQRAGMSKS
                     EKILLNALELAKTYGAVTNFIRLQDLKIYDCEGNYSHNPGFCTYPCQSSLKYEDDQMQ
                     TVYDAVLDTDILIIATPIRWNNHSALVQKFVERMNCIENSDVWFKKRLIEKKVAGLII
                     IGHVDGVQHVAGNLMNFLNWLGFHMPQDMITSWVGPNDEDTTKDWEEIRNNPYTQEDL
                     VNMVHSLLRLAYDIKNSDRELIKTGSQPP"
     gene            complement(2846..3436)
                     /locus_tag="VKL21_10870"
     CDS             complement(2846..3436)
                     /locus_tag="VKL21_10870"
                     /inference="COORDINATES: protein motif:HMM:NF022420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HMB46311.1"
                     /translation="MNQSKLHSIDGDGTGTAAVISPVGHIQHNDILISINNGKTGAIS
                     QKLFNEINSYISSQACVDPLWNSFQHRICRMLRPARENEIKNHYICIKRISIWVKNMP
                     KDMTFEKTMEAMSKMSPDEMKAKMAELTRICMCGKCPSYKGTGETKLLFCATGKSGII
                     KKEKGCVCPACPVTEKLGLRWIYYCMKGSGKEQAGL"
     gene            complement(3438..>4519)
                     /locus_tag="VKL21_10875"
     CDS             complement(3438..>4519)
                     /locus_tag="VKL21_10875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMB46312.1"
                     /translation="LLGFTDPIRPSVPAAVSECYTAGIRVIMITGDFPGTAVHIAREI
                     GLKNPDRIITGPELSQMSMAELQEKIKKTNIFARVVPEQKLAIVNSLKANGEVVAMTG
                     DGVNDAPALKSSHIGIAMGERGTDVARESSSIVLLNDDFSSIVEAVRLGRRIFDNLKK
                     AISYIFSVHVPIAGLALFPILFELPLILLPAHIAFLELIIDPACSVVFEAEKEEKNIM
                     QRPPRNLKDKLFDRKSLTRSLFQGLSVLFFVLIVFLLALYLGKTENEARTITFAMLVF
                     ANLTLIISNLSLEGSILKTFNSKNRALWMVVGGALLLFVMVLYVPVFRDLFHFSVLHF
                     VDLITILIGGIISAVWIILINTKLK"
BASE COUNT         1329 a          940 c          929 g         1321 t
ORIGIN      
        1 tacgcagcgg ggtaaagagc tactgctgga aagagcaaag tacgtcagcg acccgattct
       61 cgtgaataca gcaaagctgc ctgaactgcc agaagagagg cagccgaaac cgttggttta
      121 agtatcaagg aggtgtcaaa aatatgtgcg caaaagtctc atatctctca gacaaatata
      181 cgttatcgca aataatggat ggatacaggg aggcagtaaa gcttctttat atacacattt
      241 ttgagtatat gacagaaaag ggattcttcg gacacctatc cggcgaaata cccctgaatg
      301 accttatgga aaaaatgaac atatccaaag atagaaagca gatcttcctt gcgattagtg
      361 caaatttgag atatcttata tcgatttagt gactttcata aacagcgatg tagatgatgg
      421 actgccgttc ttagaaagcg gtaagtatga tggggttatg gcaataggac tgctacactt
      481 tcttagaaaa ccagaacaat tcttaagcga agttagccgc attctaaaac caggcggaaa
      541 gttcgccatg ggaaaccaat atctcatcaa accacttatg tatcaaggtg caactgatct
      601 gtatttctca atcataggac aatacacaag agcacataca cctgaccaaa taagacaaat
      661 gctcaattcc agcggcttaa accccaacta tacatttgaa attggaaatt acggatggtt
      721 ttatgctcga aaagaatgag cataccaccc tttattttaa taaagaggga ctttaagagt
      781 atgaatagac acgttgtaga agcaatggga agaacccgca tcacaataga agacgggaaa
      841 gtggttgagg tcggcaatcc tcggaccgga tattgcccta tctgggatag ggtaaagggt
      901 gtaaaaaaaa tcactcccca aatcgcaaag gagaatattg aatacaggat acgggaattt
      961 ggcatgttta cttccaacag gtgaattaaa atggatgtct ttgttggctt tggggcttct
     1021 gagaccttca tgactgcgct ggaatcagga ttgatagatt cctgcgtcat taattgcgat
     1081 ggtgtaggta ccgtcataac caacaatccc actttagtgc aggcggattc gtattggatc
     1141 cggtcactgc aaaactggat tccgtggctg gagttaaaaa agcaattgaa ctcggtcata
     1201 aaaaaattgg tgtacccgtg tcttctgtag aagtagcaaa agcatgcaga cagcttgaat
     1261 caaaacacag tgttgacatt attacttttg gtgtgcattt aacaggtata ggaaaataag
     1321 agtccagaga gcttatccgc cttgttgata ttactacagg ctgtatgtcc aaatatatca
     1381 ggaatggtgt ggcaggtaag gcagttgctc agttcggagt atctatacca atctttgcct
     1441 taacaaaacg tggtaagagg ttattattgg aaagggcaaa agaaatcgac tcccaaatgc
     1501 ttgtaaacac aacagaatta ccggttttac caggtgatat gcagccaaga ccgctggtgt
     1561 gaagagtttg atagtgtgaa gatattcatt taatatcata acttggggaa gtgggtaatg
     1621 gaaatcatca cggcagttct cataatactg gtatttgcaa gaatatttgg ggagatgttt
     1681 gcacgaataa aacagccatc aattatagtg aaatgatcgc tgggataatc attggtccat
     1741 ctattctcaa tctaataagt cctgaagtaa agggcatcga tatcttagcc gaatttggag
     1801 ttttttttct cctatttttt gcaggaatgg tattggacta aatgccaggg gattgatggg
     1861 tctggttata tcagaagtag gattgaaata tggattgata gacatgaaca tatatacaat
     1921 gctagtcacg atgagtataa tcactacttt tgtaacgcct tatggattga aaagaatatt
     1981 taaatgataa tgcatttgat ctggctgctt ccgtattctt ttccgccctc gtatgaagca
     2041 gaaatcaata gaaccaaaga aaagttaagg gggttgactt cctgtcttaa tgagttctct
     2101 atcactattc ttaatatcat atgccagcct caaaaggctg tgcaccatgt tcacaaggtc
     2161 ttcctgggtg tagggattat tccggatctc ttcccagtct ttcgttgtat cttcatcgtt
     2221 cgggccaacc catgatgtga tcatatcctg cggcatatgg aagcccagcc agttgaggaa
     2281 attcatgaga tttccggcaa catgctggac gccgtccaca tgtccgatta ttatcaatcc
     2341 tgctactttt ttttcgatca gtcttttctt gaaccataca tccgagttct cgatgcagtt
     2401 cattcgctcc acgaactttt ggaccaatgc agagtggtta ttccatcgga tcggcgtggc
     2461 aatgatcaaa atatcggtgt ccagtactgc atcgtaaact gtttgcatct gatcgtcttc
     2521 atatttcaat gaactctggc acgggtaggt gcaaaaaccc ggattatggg aatagttgcc
     2581 ttcgcaatca tatatcttaa ggtcctgcag gcgtatgaaa ttcgtaacag ccccataagt
     2641 tttcgccagc tcaagtgcat ttaataaaat cttctccgat ttactcattc ccgctctttg
     2701 cctggaagaa cccgatatgc ccagaacttt taatcctttg cattttttca ggtcaggagg
     2761 caagatcaaa tggatgccta atttcctgcc gctcggatgc gctgtcatgt ggaatcagag
     2821 atttcaattt ccagtttatc cgtatttaca acccagcctg ctctttgcca gaacctttca
     2881 tgcagtaata tatccatcta agacccagtt tttcagtaac aggacaggcc ggacatacgc
     2941 agcctttctc ttttttaata attccgcttt ttcctgtcgc acagaataat aatttagtct
     3001 cacctgtccc cttatagctt ggacatttcc cgcacatgca aattcttgtg agttccgcca
     3061 ttttggcttt catctcatca ggagacattt tactcattgc ttccatggtt ttttcaaaag
     3121 tcatatcctt tggcatattt ttcacccata tacttattcg ttttatgcat atataatgat
     3181 ttttaatttc attttccctt gccgggcgga gcatgcggca tatacggtgt tggaatgaat
     3241 tccacagagg gtcgacgcat gcctgactgc tgatataact attaatctca ttaaataact
     3301 tctgtgagat cgcccctgtc tttccgttat ttatggaaat caaaatatcg ttatgctgta
     3361 tatgtccgac aggcgagatg acagcagcgg tccctgtccc gtcaccatct attgaatgca
     3421 atttactttg gttcatatta tttaagtttg gtgttgatca gtataatcca cactgcgcta
     3481 atgattcccc cgatcagtat tgttatcagg tcaacaaaat gcagaacgga aaaatggaat
     3541 aggtctctga agacaggtac atacaatacc ataacaaata agagaagcgc tcctcctact
     3601 accatccata atgccctgtt cttcgaattg aatgttttta atatacttcc ttccaatgat
     3661 agatttgaaa ttatcaacgt tagattggca aatacgagca tagcaaatgt aattgtacgc
     3721 gcttcattct ctgtcttgcc caaatataat gccagaagaa atactatcaa tacaaaaaaa
     3781 agcacgctca atccctgaaa caggcttctg gtcagacttt ttctgtcaaa taacttatcc
     3841 ttgagattcc tgggcggtct ttgcatgata tttttctcct ccttttcagc ctcaaagacc
     3901 actgaacacg cagggtctat tatcaattca agaaaagcta tgtgcgctgg taaaagaata
     3961 agaggtaact cgaataagat cggaaaaagc gccagacctg caattggcac atgaactgaa
     4021 aaaatatagg aaattgcttt cttgaggtta tcaaaaattc ttcttcccag cctgacagcc
     4081 tcgacaatgg atgaaaagtc atcgtttagc aggacaatgg acgaagactc tcgcgccacg
     4141 tccgtgcccc gttctcccat tgcgatacct atgtgggaag atttcagagc aggagcgtca
     4201 tttaccccat ctcctgtcat tgcaaccact tctccattcg cttttaagga attaacgatc
     4261 gccagttttt gttccggcac cacacgagcg aaaatattag ttttcttgat cttttcctga
     4321 agttctgcca tgctcatttg agacagctca gggccagtaa tgatcctgtc cgggtttttc
     4381 aaaccgattt ccctggcaat gtgtacggct gttcccggaa aatcccctgt tatcattatg
     4441 actcttattc ctgctgtgta acattcagaa actgctgcag gaacagaagg acgaatcggg
     4501 tctgtaaaac caagcaatc
//
LOCUS       DATGBN010000431         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_539200, whole genome shotgun sequence.
ACCESSION   DATGBN010000431 DATGBN010000000
VERSION     DATGBN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539200"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..590
                     /locus_tag="VKL21_10880"
     CDS             399..590
                     /locus_tag="VKL21_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46313.1"
                     /translation="MAGYAGLFQALIVQKKCRDILPKGNYPAGLVLFSRRSRKRRRSN
                     IFICINLWIIGKINTWDGI"
     gene            complement(674..1282)
                     /locus_tag="VKL21_10885"
     CDS             complement(674..1282)
                     /locus_tag="VKL21_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMB46314.1"
                     /translation="MKVNKKLTLRGIGNPVVDARGSGSAITLSANGITLEGFTAIGSG
                     HGSFFESPEAGIRITSNRNTLTGNNVSSNNDNGISLNSDYLYSSSIDNTIYNNIFNNT
                     NNIKFYDPDINTWNTTRRSGTNIIGGSLLGGNFWAYPNGTGFSQTCRDVNSDGICDQS
                     YILNTKNIDYLPISMNFTNDKMPPKSVSNLKNVSYASAWTAN"
     gene            complement(1462..1605)
                     /locus_tag="VKL21_10890"
     CDS             complement(1462..1605)
                     /locus_tag="VKL21_10890"
                     /inference="COORDINATES: protein motif:HMM:NF036315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5371 family protein"
                     /protein_id="HMB46315.1"
                     /translation="MKYIHVQSVLPQEDVIALKEKSRESSVKEAISKAVYHYLKCKLA
                     GKN"
     gene            complement(1864..1946)
                     /locus_tag="VKL21_10895"
     tRNA            complement(1864..1946)
                     /locus_tag="VKL21_10895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1910..1912),aa:Leu,seq:caa)
     gene            2102..3454
                     /gene="cca"
                     /locus_tag="VKL21_10900"
     CDS             2102..3454
                     /gene="cca"
                     /locus_tag="VKL21_10900"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HMB46316.1"
                     /translation="MTDQNTILASIISSIRPSNKERHRLRTLAQRLIERINSIGKADG
                     LNVNGILVGSSARGTWISGEHDLDIFIMFPPDVERHYLEEKGLYIARKIALEGDSFEE
                     RYAEHPYIHAVIDGFEVDLVPAFNVENAAQIKSAVDRTPFHNKYVESRIGGLEDDVLL
                     LKQFQKGMGVYGSELKTHGFSGYLVELLVIYYGSFEKVLKAACDWKFVTLIDIENHGI
                     INHKDPLIMIDPTDPARNVAAALSLDNMCIFMDRACDFLKNPDESYFHISIVQPLGDK
                     DLEYLISARGTCLIAVEFEAPDEVEDVLFPQLYKLEGSCHEMLERYDFRVYNSGVWAK
                     EKAVVLYELESAKLPNVKKHTGPMVGTKEHASAFKSKYDCANKLSNVYIRNGKYVVEI
                     PRKYSSARKLVESEILKCGLGKHIGVRMKKKFNVMENLEILSIRDEGFRKFLRRFFDK
                     "
BASE COUNT         1106 a          649 c          756 g         1124 t
ORIGIN      
        1 ccccttgtac gacattatta accttaaatc tttctattga taacattata atcaataata
       61 aattataata caaaaataat ttaataatgt tctgttccaa acatttctgt ttataatatt
      121 tcacagtaat atttatttat cctgaagtcc ttgatatatc aggggaagta ttgggagaaa
      181 ttgggttatg tcagtaaaat tactcgaaag agtaggatta ttaagatcgg ttatcatatt
      241 ttcgcataat taaatagtac aaagtccatt aattaagaag aattgctggg agtttctgga
      301 atgtggtaga gaacctggtg gagaaaaagc agaaaaatcc tgtatatgtc ctgcctcaat
      361 agacacttcc gcagatggat tgaacggagg aaaaaacggt ggcaggatat gctgggctat
      421 ttcaggcact tattgtgcaa aaaaaatgca gggacatttt gccaaaaggc aattatcctg
      481 caggtcttgt tcttttttca agaaggtcaa ggaagaggag gaggtcgaac attttcattt
      541 gcataaacct ctggatcata ggaaaaatta atacgtggga tgggatttga atccacgaac
      601 tcctgcgaga atatatcttg agtttgtctc ctttaaacgg tacctggtgt gtaccttgga
      661 gataggcatt tcatcaattc gccgtccatg ctgaagcata acttacattc ttcaggttgc
      721 ttacgctttt tggaggcatc ttatcgttcg taaaattcat tgaaataggc aggtaatcga
      781 tattttttgt attaagaata tagctttgat cacatattcc atcactattc acatcccggc
      841 aggtctggct gaatcctgtg ccgttaggat atgcccagaa gttgccgccg agcaaggatc
      901 ctccgatgat gttagttcct gatcgccttg tggtgttcca tgtattaata tctgggtcat
      961 aaaatttaat attgttggta ttattaaaaa tgttgttgta tatcgtgttg tcgatgctgg
     1021 aagagtacaa atagtctgag ttcagagaga tgccattgtc gttgttcgaa ctgacattgt
     1081 tgccggtcag tgtgttgcgg tttgaagtga tcctgatacc cgcctcggga gattcgaaaa
     1141 aagagccatg cccactccca atcgctgtaa atccttcaag tgtaattcca tttgcagaaa
     1201 gcgtgatcgc gcttccactt cccctggcgt ccaccacagg attgccaata ccgcgaaggg
     1261 ttagcttctt gtttaccttc actttttcta aataagtacc gctttttact aaaatagtat
     1321 ccccactgct tgaagccttt atcgctttct gtagtatatt aaataagcag gttaagtttc
     1381 cctgttgttt aactgttctt gaaatatgtg aaagatgcag agtggaactc agctaactca
     1441 gacctataat tcaaagatgt tttaattttt gccggccaac ttacacttta aataatgata
     1501 cactgccttt gagatggctt ctttaactga agactccctg gacttttctt tcaaagcgat
     1561 tacatcttcc tggggaagta ctgattgaac atggatatat ttcatattac tatcccgtgc
     1621 ttatgggtgg gggagaggaa ggaaaatttt atttttggat tgttggataa aactacttcc
     1681 tcattttaat tatagaacct tacccaatat aagtattctg acaaatattt gagtttattt
     1741 tttgttttaa gagttctgta ctcctttttt ttgtttttat tagttcataa tgaactttat
     1801 atccataaaa cagaaaaccg tacaaatcaa tcgtgtaatg ggaacagggg aaattagcat
     1861 gaatgcgggg gaagggattt gaaccctcga actcctgcga gaatagatct tgagtctatc
     1921 acctttggcc gcttggttac ccccgcaact ggggcattca gaattatcct tatttgactt
     1981 aaggatgtcg ttaggcaaat tagaagtgcc agaaagttct cctatgacaa aaaattaaac
     2041 cgatataaag agtctattta taatatatta gataatcata tccataattt actatataaa
     2101 gatgacagac caaaacacaa tactagcttc gataatttca tcaataagac catccaataa
     2161 agagaggcac aggttaagaa cacttgcaca aaggctcatc gagcgcatta attctattgg
     2221 caaagccgat ggtttgaatg tcaatgggat cctcgtagga tcttctgcgc ggggaacatg
     2281 gatatcagga gagcatgacc ttgatatctt cataatgttc ccacccgatg tagagcggca
     2341 ttatcttgaa gaaaaagggc tttatatcgc ccgcaagatc gcgctggaag gggactcttt
     2401 cgaagaacga tatgctgaac atccgtatat ccacgcggta attgatggtt ttgaagttga
     2461 ccttgtcccg gctttcaatg tagagaacgc cgcgcagatc aaatcggcag tagacaggac
     2521 gccctttcat aataagtatg tggaatccag aatcggaggt cttgaagatg acgtattgct
     2581 tttgaagcaa ttccagaaag ggatgggagt atatggctct gaattgaaaa cacacgggtt
     2641 ttcaggttat cttgtggaat tactagtgat atattatggt tccttcgaaa aagtactcaa
     2701 agcggcttgc gactggaaat tcgtgacatt aattgatatc gagaaccatg gtattattaa
     2761 tcataaagat cccctgatca tgatcgatcc caccgacccg gcacgaaatg tcgctgctgc
     2821 gctatctctt gataatatgt gtatatttat ggacagagca tgtgactttc ttaagaatcc
     2881 cgatgaatct tattttcata tcagcattgt gcaacctctt ggggataaag atctggaata
     2941 tctcatttcc gcaagaggca cctgccttat tgctgttgaa tttgaagccc cggatgaagt
     3001 tgaggatgtc ctgttccccc agctttataa acttgaaggt tcatgccatg agatgctgga
     3061 acgatatgat tttcgtgtct ataacagcgg agtatgggct aaagaaaaag ccgttgtact
     3121 atatgaactt gaatccgcaa agctcccgaa tgtaaaaaag cataccgggc caatggtggg
     3181 aacaaaagaa catgcatctg cattcaaatc aaaatatgac tgcgccaata agttatctaa
     3241 tgtatatatc cgtaacggta agtatgtggt tgaaattcct agaaaatatt ccagtgccag
     3301 gaagcttgtt gaatccgaaa tactcaaatg tggtcttgga aagcatattg gtgttagaat
     3361 gaagaaaaag ttcaatgtta tggaaaattt ggaaatacta agtatcaggg atgagggatt
     3421 caggaaattc ctgaggagat ttttcgataa atagaggcat aaatcatgaa atatttacct
     3481 gtaatactca ttctattatt cttgttgatt cccggatgtc ttgaatccag cgcttataaa
     3541 gacatttcaa gatcgcaatt atcatcgaat ccggatgttt tcgaagggaa aaaaatctgt
     3601 actgatctaa tatatgaaaa taatggtttt acagg
//
LOCUS       DATGBN010000432         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_345595, whole genome shotgun sequence.
ACCESSION   DATGBN010000432 DATGBN010000000
VERSION     DATGBN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345595"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..986
                     /locus_tag="VKL21_10905"
     CDS             51..986
                     /locus_tag="VKL21_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195338.1"
                     /note="Replication protein A protects and stabilize the
                     intermediate ssDNA that is generated by the unwinding
                     action of a DNA helicase at the replication fork. In
                     addition, SSBs prevent the formation of secondary
                     structures by single-stranded template DNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor A"
                     /protein_id="HMB46317.1"
                     /translation="MSEITRQIEARFLELGIKVETSEIDTKLDQLINKFKVPPEEARR
                     NVINYFLKKHNIPKSEFFTPRETPQVKASDVKEDGKWVTLRGKVVQLWESNSESISQV
                     GLFGDESGTIKFTKWTKSGLPNVEEGKNYLFKNVTTSQYQGQFSIKLNRTSEIIPIEE
                     NIEVGTTLVEFSGAIVDVQSGSGLIKRCPECKRALIKGACSEHGKVEGVYDLRIKAVL
                     DDGEKVQDILMNREITEAFTGISLEKAKEMATEALDQGVVLDVMKSKLVGRYYVVAGK
                     KLDRFILVDSIKLDVKPIEEDIARILAAAPVAAEA"
     gene            989..1561
                     /locus_tag="VKL21_10910"
     CDS             989..1561
                     /locus_tag="VKL21_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMB46318.1"
                     /translation="MAENGSGYVREISHRVFAAELKESNLQSKEGQDQYSPQYLITPT
                     GAKCNRIFIVGTLTEKEDVGTDSEFWRGRIVDPTGTFYVTAGQYHPEAVQVLAKTLPP
                     EFIAVIGKPTTFTTKEGNILTSIRAESMHVVDAATRDRWVVETANLTTKRLARLQGND
                     PDAVKAREHYSTDVEQYKAMVAMAIESRAR"
BASE COUNT          694 a          448 c          527 g          588 t
ORIGIN      
        1 agagttgcct ctcaaataca aaaaattaaa ctggaggctt gagaaaaaat atgagcgaaa
       61 taacaagaca aatagaagcc cgattcttag aactgggcat aaaggtagag acgagcgaaa
      121 tcgatacaaa gcttgaccag ttgatcaaca agttcaaggt cccgccagaa gaagctcgcc
      181 ggaatgtaat aaattacttt ttaaagaaac acaacatccc gaaatctgaa tttttcaccc
      241 cgcgggaaac accgcaggta aaagcatccg atgtcaaaga ggatggaaaa tgggtgacat
      301 tgcgcggtaa agttgtgcag ttatgggaaa gcaattctga atccatttcg caggtgggat
      361 tgttcggaga tgaatcaggg acaataaaat tcaccaaatg gactaaatcg ggtttgccta
      421 atgtcgaaga aggaaaaaat tacctgttca agaatgtaac cacttcgcaa tatcagggac
      481 aattcagcat caagctcaac aggacaagtg agataatccc gatagaagaa aatattgaag
      541 tagggacaac cctggtcgaa ttctcaggtg ctatcgtgga tgtccagagc ggctcaggcc
      601 tgataaagag atgtcctgaa tgcaagcggg ctcttataaa aggggcatgt agcgagcacg
      661 gaaaggttga aggggtctat gacctgcgaa taaaagctgt ccttgatgac ggcgaaaagg
      721 tgcaggatat cttgatgaac agggaaataa cagaagcgtt taccggtatc tcccttgaaa
      781 aagcaaagga aatggcaaca gaagctcttg atcagggtgt tgtgcttgat gttatgaaaa
      841 gcaagctcgt ggggcgctat tatgtagtgg ccggaaagaa acttgacagg ttcatactgg
      901 tcgattccat caaactggat gtaaaaccga ttgaagagga catagccagg atacttgcag
      961 cagcgcctgt tgcagcggag gcgtgatcat ggctgaaaat ggatcaggat acgttcgtga
     1021 aatatcgcac cgtgtatttg cagcagagct gaaagaatca aatctccaat caaaagaagg
     1081 acaggatcag tattcaccgc agtatctgat caccccgaca ggagcaaaat gcaatcgcat
     1141 cttcatcgtg ggcacgctca cagaaaaaga agatgttggg actgactccg aattctggcg
     1201 tggtcgcatt gttgacccca caggcacttt ctatgtgaca gccgggcagt accatccaga
     1261 agcagtgcag gtgcttgcaa agacattgcc ccctgagttc attgcagtta tcgggaagcc
     1321 aacgactttt acaacaaaag aaggaaatat ccttacttcc ataagggctg agtccatgca
     1381 tgttgtggat gccgcaacac gcgataggtg ggttgttgag actgcgaacc tgaccacaaa
     1441 gaggcttgca agattgcagg gcaatgaccc tgatgctgta aaggcgaggg agcattattc
     1501 aacggatgtg gagcagtata aggcgatggt tgcgatggcg atcgaatcga gagcacgata
     1561 ggagatagtc ctaccctttt atttatcttt ttattgacgg ttcctttggt caatctttca
     1621 gcaatattct tcagtaaaca ttgatattgc ccataatgct cccctgaaaa tccctcattt
     1681 tagggtaccg ccccgatctc tatgtacacg cacgctaaga gactaggcat tctgtttttt
     1741 gccaaggttt ctgtcttcaa cttttcttgt tctgctgaag tttcttcaat cccacgcatc
     1801 aaccgcatgc ttttttttca tcagcaaatt ccttagcgtg catctacgtt aaaatcgggg
     1861 cggtaccctt tttacacagg aacagtttag cccaggtttc aaaattatca gatagtaaaa
     1921 tcaagagagt caaggaataa atattttggt aggtaattat ttaattactg tcttatggac
     1981 actttacgga cgaatcgtgg tgataccctt attaatcggc agtggcataa tttaagatac
     2041 ttaattcctt tttattagat tagacagttg cttcgcctac tcatgttgat tccaattttt
     2101 cgcaaatatt tggtttaagt aatcttccga tagtgaattg ttctgaaatt gggcaaattc
     2161 ctagaaggaa aggttattca aatatcatta agtatcgtat tctggcacac tgccaaagat
     2221 tgggttacta atccttatat ttcactattt ccgcatt
//
LOCUS       DATGBN010000433         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_500474, whole genome shotgun sequence.
ACCESSION   DATGBN010000433 DATGBN010000000
VERSION     DATGBN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500474"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VKL21_10915"
     CDS             complement(<1..214)
                     /locus_tag="VKL21_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46319.1"
                     /translation="MVRNKQAIETYVNLLSVSFTFVSALPFMEKNYAKYKFESPQIIK
                     RVISSHLMQELILGSFVEKLKTTKIYY"
     gene            1171..2130
                     /locus_tag="VKL21_10920"
     CDS             1171..2130
                     /locus_tag="VKL21_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46320.1"
                     /translation="MMQIIKYPIKEVYLNTIPDNKMLSLGITQTQKLLCEKSIRQYGL
                     LTPIVLMENPTGELITLTGENELEVLKEMAVDKIEVFVTSLKNRSDSGKMILLLSSFK
                     TGLNPISEGLILRELIKVGAYTQKELAITLMKSKSWVSKRLTLAEQLNEQVSEMVLSK
                     KVCPGSAQEIARLPKEVQFKFATHVYERDMTKASVELLVVTYNNRGTSQSLKNAIIHQ
                     PDAILHTLAPQKVIKTVNQDIPDDGFKKFNGSLRLLLSLLSEVEIHLAGMSKEELFQH
                     QRLLPIIQKACSRFQNLMSYHIDSLGKLYDSEITQGDDFIGNN"
     gene            2117..>3279
                     /gene="istA"
                     /locus_tag="VKL21_10925"
     CDS             2117..>3279
                     /gene="istA"
                     /locus_tag="VKL21_10925"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HMB46321.1"
                     /translation="MATIDINTYKLIRRLHAVEKLSQRQIAQKLGVSRNTIRKYCLGA
                     THPDQKNAYHIEKSPLRVTLEEEILEMVNAHKCAPRKQQLNGKIIWQTLVSKGFDIGE
                     STVRKYIQELRIDQPNIFIPLEFEPGEAMEFDWGDVYAYVNKLKTRVSVFCAVLPYSY
                     GIFCAVFPDKTYASFFTGHVMAFEYFNGVPKRCIYDNLKTAVLEGSGKDAVKQEKFKN
                     FEAHYAFESVFCNAAAGWEKGSIENLVAIVRKIAFTPMPSFSNFQELQEHVSLKCSEY
                     CMMHKIKDRSRSIKEMLEEEKTYLLPLPMYHLDTEEEIKATVHSDLTVRLNTIKYSVP
                     AAYVGLSVTLKVSPFHVTVYHDGKLLYKHRKGLNSSDHQYIPEHYLEILLRKP"
BASE COUNT         1052 a          658 c          607 g          962 t
ORIGIN      
        1 tgtaataaat tttagtagtt ttgagtttct ctacgaaact ccctaaaatt aattcctgca
       61 tcagatggct tgatatcaca cgttttatga tctgtgggct ttcaaattta tacttggcat
      121 aattcttctc cataaatggc aaagcactta caaacgtgaa ggaaacagac agcaaattaa
      181 cataagtctc tattgcctgt ttatttctca ccatgtaatt ggatacgcta cgatttaact
      241 gagccaaaat cataaccact gcttctataa aagtatacca acctatggta tacttttttt
      301 agcgtgctac gatttcataa atccacctta gtgagccgcc gtcataatca aggttttgat
      361 tcatgtgcaa aatattgaaa aggaaaaaag aactttgatg ttgttatgtt aaatgtacta
      421 ccatttaacc atcaaagttc ttccccatat aaacaagatg attatatcat tttcagaaga
      481 aactttcaat ctaaacgatg atgatttatt tgtttttttt acggattcat ttttagaaaa
      541 cctgtcttta aaaggagatc tgcttaaaaa aaccataatg tataaatata ttctaaacaa
      601 tcgcgaacgt acacttgttt gtccattgat taaaaatgta atgggtgata tcgaggtgat
      661 ttggcctgcc tttaaactgc cattcagaaa atatccgatc tgtgtctatt tatacgccgt
      721 catcctatac ctttcttcca atcttagtat gcgtaaggtg tgcacaaaag tcagaaagat
      781 atttggtatt acggggtttt gtcattcaac gttatcacga agcttaaaaa aactttcttt
      841 catagcagat cagattggaa gagttcttca tcaacacgaa atcagcgaca tccatgagcc
      901 caatcaagac cataaatcca gcctatcatc gcttcgagtg aatccttcca tacaaaaaca
      961 ttttaatccg tttcgtaatg agcaacatct gaaattacga acacttctta atcctatcct
     1021 tgattcctgc cagagcgccc catttagcaa ccatatcaac gttctttttt acaaaaaata
     1081 caaaaaattt ctgctgtaag tctttaaatt catcaaccac tatagctgtg caccaacatt
     1141 cacgttacga tggttgcaag gaggtggtct ttgatgcaga taatcaaata tcccataaaa
     1201 gaagtatatc ttaataccat acccgacaac aaaatgctgt cattgggtat tacccagacc
     1261 caaaagctgc tctgtgaaaa gagcattcga cagtatggac tcctcacgcc cattgttctg
     1321 atggagaacc cgacaggtga gctcataacg ctgacaggcg aaaatgagct tgaagtttta
     1381 aaagaaatgg ctgttgataa aatcgaagta tttgtgacca gtctcaaaaa ccgttccgat
     1441 agcgggaaaa tgattctgtt actttcatcc ttcaaaacag gattaaatcc catatcggaa
     1501 gggctgattc taagggaact gataaaagtg ggtgcctata ctcaaaaaga gctggcaatc
     1561 acgctgatga aatcgaaatc atgggtttct aaacggctta cgctggcaga acagttaaat
     1621 gagcaggtgt ccgaaatggt tttatccaag aaggtctgcc cgggaagcgc ccaagaaata
     1681 gctcgacttc ccaaagaagt tcaatttaag tttgccactc atgtctatga aagagatatg
     1741 actaaagcaa gtgtggagtt attggtggtg acctacaata acagaggaac ttctcaatcg
     1801 ctgaaaaatg ccatcatcca tcagccagat gcgatcttac atactttagc acctcaaaaa
     1861 gttatcaaaa ccgttaatca agatattcct gatgatggtt tcaaaaagtt taatggttcg
     1921 ttacgtcttt tgctgtctct tctatctgaa gtagaaattc atcttgccgg catgagtaaa
     1981 gaggaacttt tccaacatca gcggttactg cctatcatac aaaaagcttg cagtcgattc
     2041 cagaatctta tgagctatca tatcgattcc ctcgggaaac tgtatgattc agaaattaca
     2101 cagggagatg atttcattgg caacaattga tatcaacacg tataagctta ttcgcagact
     2161 tcatgcagtg gaaaagctga gccagcggca gattgcacaa aaactgggag ttagtcgcaa
     2221 tacaattaga aagtactgcc tgggtgctac acatccagat caaaaaaatg catatcatat
     2281 tgaaaaatca cctctaagag taacgttgga agaagagatc cttgaaatgg ttaatgcaca
     2341 taaatgtgca cccaggaaac agcaattaaa tgggaaaatc atttggcaaa cgcttgtttc
     2401 caaaggcttc gatattggag agtccacggt gcgcaagtac atacaagaac ttcggattga
     2461 ccaacccaat atctttatac ccttggagtt tgagcctggt gaagcgatgg aattcgattg
     2521 gggtgatgtc tatgcttatg ttaacaagtt gaaaacccgt gtgtctgtct tctgtgctgt
     2581 tcttccttac agttatggca tcttctgcgc tgttttccca gacaaaacct atgccagctt
     2641 ctttacggga catgtcatgg catttgaata tttcaatggt gttcctaaac gatgtattta
     2701 cgataaccta aaaacagctg tactggaagg gtccggaaaa gatgctgtaa aacaagagaa
     2761 attcaaaaat ttcgaagccc attacgcttt tgaaagcgtg ttttgtaatg ctgcggctgg
     2821 ttgggaaaaa ggcagtattg aaaatcttgt tgctattgtg cgtaaaattg cattcacgcc
     2881 catgccttct ttttccaact ttcaagagct ccaagaacac gtctctctca aatgctcaga
     2941 atactgcatg atgcataaaa tcaaagaccg atctcgctct attaaagaaa tgctggaaga
     3001 agaaaaaact tatttattac cgctccccat gtatcatctt gacacagaag aagagatcaa
     3061 agcaaccgtc cattccgatt taacagtccg tttgaacacg atcaagtact ctgttccggc
     3121 tgcttatgtg ggtcttagcg tcacgctgaa agtgtctcct tttcatgtca ccgtttatca
     3181 tgatggaaag ttgctctaca agcataggaa aggtttaaat agttcggatc atcaatatat
     3241 tccagagcat tatcttgaaa ttcttttaag aaagcccca
//
LOCUS       DATGBN010000434         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_461923, whole genome shotgun sequence.
ACCESSION   DATGBN010000434 DATGBN010000000
VERSION     DATGBN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461923"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VKL21_10930"
     CDS             <1..388
                     /locus_tag="VKL21_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HMB46322.1"
                     /translation="KGDDLIHFIVERFDSPASMRLAYYMQRLLIVCIRDVLGEHEIRT
                     TRNGDDLFVNEKKLTVSIASAGVTSEKIHCGINITTQGTPCDIRTAGLLDLRIKDWKV
                     PAKEIAELFIREIDDIESDIVKTKSL"
     gene            430..1344
                     /locus_tag="VKL21_10935"
     CDS             430..1344
                     /locus_tag="VKL21_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46323.1"
                     /translation="MVEDIGKIISNGFGTYMRNPNICVPFVLNFILSFSLALIMIVFV
                     FAYVMGSSLSTLENAKSPEAIAAIMFPLISGHIIEIAILVMIISLVVLFLQAYFISGA
                     IGMAIQANESGRSSVNTMKDAGKKNLMNMFFAQLLFGLLSLAGIVFIVPGAMKTDISQ
                     IFTSENTGGVALLLGGFFLWMIYLVILGILLSVFSYALVADNLGPLESMAKSVSFFKQ
                     NKIDVFLLFLTIFFIGIAFVIIDAIIGSIIVNNFLKESINQFISLIIIQPLTTVLWVR
                     LYMAKTNKKFYIDELLAHPNELMETQKE"
     gene            1480..2550
                     /locus_tag="VKL21_10940"
     CDS             1480..2550
                     /locus_tag="VKL21_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMB46324.1"
                     /translation="MTLLEEFIAEMEANGLAKNTIVSYGSILKRLERYINSLDKNNIL
                     LEEYKSLDLITTDELKAYFKLLKSEMTESSFALHQISIKNFYKKIGKPDIVQWIVKIK
                     PKETLKSDDILSTDDINKMLEATDSHYYKAFIAFLYEAGCRFSEAHCLKYKDFTETNE
                     GMIVNIPTKKTSAGYRNTILPFSSEYIRNLKVYLSAKSEDIVFSVGNWASNDMLHNIA
                     TKAGITKPVNCHEFRHAQATAMVQLGYNEAIIRKKLGWSQTSGMIARYQHLNDEDVIN
                     ATLSNTGKIPKTAVRTEIKEAEKLSLVDAAMQLSKLSEENRELKQEVEIMKASISEDM
                     IKAMINARVNELMKKNPEKNDA"
     gene            2979..>3231
                     /locus_tag="VKL21_10945"
     CDS             2979..>3231
                     /locus_tag="VKL21_10945"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMB46325.1"
                     /translation="MKKETENWWKQANRDLESAKKIMELGEYHVCAFLFQQAVEKALK
                     ALLIERTGNFPRIHDVVELSRRVKAPSRITELCATINPAY"
BASE COUNT         1127 a          499 c          646 g          959 t
ORIGIN      
        1 taagggtgat gacttaattc attttattgt ggaaagattc gattcgcctg caagtatgcg
       61 ccttgcttat tacatgcagc gccttcttat cgtatgcata agggatgtac tgggagaaca
      121 tgaaataaga acgacccgca atggcgatga tctttttgtg aatgaaaaaa aactcacagt
      181 aagcattgcc agtgccggtg taacaagtga aaaaatacat tgcgggataa atatcacaac
      241 acaggggaca ccttgtgata ttcgcacagc ggggcttctt gatcttagga taaaagactg
      301 gaaagtccct gcaaaagaga tcgcggaact ttttatccgg gaaatagatg atattgagag
      361 cgatatcgta aaaacaaaaa gcctataaat aattaagtcg ttgagtcaaa actaaaaagg
      421 ataatgaata tggtggaaga tatcggcaaa attatcagta atggctttgg tacatatatg
      481 agaaatccga acatctgtgt tccttttgtt ctcaatttta tcctgtcatt ctcactggcc
      541 ctgattatga tcgttttcgt gtttgcatat gttatgggct catctttatc gactcttgag
      601 aatgcaaaga gtcctgaagc tattgccgcc ataatgtttc ctcttatttc cgggcatatc
      661 atagaaatcg ctatccttgt tatgataatt tcactcgttg ttcttttcct ccaggcatat
      721 ttcatttcag gagctatcgg aatggcgata caggctaacg aatccggaag atcctcagtg
      781 aacacgatga aggatgccgg aaaaaagaat cttatgaaca tgttttttgc acagttgctc
      841 ttcggtttgc tttcacttgc gggcatagtt ttcattgtac cgggagcaat gaagactgat
      901 atttctcaga tatttacttc agaaaatacg gggggtgttg cattactatt aggcgggttt
      961 tttttatgga tgatatattt ggtgattttg ggcattcttc tgtccgtttt tagttatgct
     1021 ctcgtcgcag ataatcttgg gcctttagaa agtatggcaa aaagtgtttc ctttttcaaa
     1081 caaaataaga tagacgtatt tttgctattc ctgacgattt tttttattgg cattgctttt
     1141 gtaattattg atgcaattat agggtcaatt atagtaaata atttcttgaa ggaatctata
     1201 aaccaattta tttcattaat aatcatccaa ccacttacaa ccgttttgtg ggtcaggttg
     1261 tatatggcca aaaccaacaa aaaattttat atcgatgaac ttctggctca tccgaatgag
     1321 cttatggaaa cgcaaaagga ataattcaga tgtgaatggc gcgttgaaca aataaaaaaa
     1381 gcaatcctaa tatatacctt attacccata gtaacaaata taaatcacat attaccaaaa
     1441 agcttatttt gttctaaata atttatagct ttaggtatta tgacattact tgaagaattt
     1501 attgcagaaa tggaagctaa tggactggct aaaaacacga ttgtaagtta tggaagtatt
     1561 ttaaaacgac ttgaaagata tataaattca cttgataaaa ataatatact acttgaagaa
     1621 tataaatcat tggatttaat tacaactgat gaattaaaag catattttaa attgctaaag
     1681 tctgaaatga cagaaagtag ttttgctttg catcaaatct ccataaaaaa tttttataag
     1741 aagataggta aacctgatat tgttcaatgg atagttaaaa ttaaaccaaa agaaaccttg
     1801 aaaagtgatg atattctttc cactgatgat ataaacaaga tgcttgaagc tacagattca
     1861 cactattaca aggcttttat tgcattctta tatgaagcag gatgtagatt ttcagaagca
     1921 cactgtttaa aatataagga tttcacagaa acaaatgaag gaatgattgt taatatccct
     1981 actaagaaaa caagcgcagg atatagaaac acgattctac ctttttcttc cgaatatatc
     2041 cgaaacttaa aagtgtatct aagtgcaaaa tctgaggata ttgttttttc tgttgggaat
     2101 tgggcatcta acgatatgct tcataatatt gcaacgaaag caggaattac aaaacctgtg
     2161 aattgccatg agtttagaca tgcgcaagca acagcgatgg ttcaacttgg atataatgaa
     2221 gcaattataa ggaaaaagtt agggtggagt caaacaagtg ggatgattgc acgatatcag
     2281 cacttaaatg atgaggatgt tatcaatgca acattatcaa atactggaaa gattccaaaa
     2341 acagctgtaa gaactgaaat aaaagaagca gaaaaattaa gtctcgtaga tgcagccatg
     2401 cagttaagta agctttcaga ggagaacagg gaattaaagc aggaagtaga aatcatgaaa
     2461 gcgtctatca gtgaagatat gataaaagcc atgattaatg caagggtaaa tgaattgatg
     2521 aaaaagaatc ctgaaaaaaa tgacgcataa aaaaatatgt cgaaaacaat cccggttaga
     2581 atttatgaat ctgattgtga gtggttaatg aatcatcgca caagcaaaga aaaatcaatt
     2641 cctgaggtta tcaggggttt gattctattc aaggagcaat tggaatagct tgagcaatac
     2701 atctcttcga tcaataaaat cccatacaat gaacggagcc tgtacataat gcaacaactc
     2761 gtattaaggt aataatattt tctatcgcaa catatcttga tcagagcagc caggcgcggg
     2821 tcataactga tgcagggcgc ggtgtggcca agaatagagg tgcagacttc ccattttttt
     2881 tttctgaaaa gatagtttca cttcgctata ataatcttta taaagatgga ggttatttaa
     2941 agaagctatg aaggtggaga aaagggggag aagatgtgat gaaaaaggaa actgaaaact
     3001 ggtggaaaca ggctaataga gatcttgaga gtgcaaagaa gattatggaa cttggagagt
     3061 atcacgtttg tgcattccta ttccagcagg ccgtggaaaa agcattgaaa gcacttttga
     3121 tagaaagaac aggtaacttt cccagaatac atgatgttgt cgaactttca agacgcgtta
     3181 aagcgccttc gagaataaca gaattatgtg ccacgataaa tccagcctat a
//
LOCUS       DATGBN010000435         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_461979, whole genome shotgun sequence.
ACCESSION   DATGBN010000435 DATGBN010000000
VERSION     DATGBN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461979"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VKL21_10950"
     CDS             complement(<1..461)
                     /locus_tag="VKL21_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46326.1"
                     /translation="MAGRNKAIDIGNTLRSKALDLEGKQKQSGNVLTSDMEKEIDTFL
                     AKNTLPTEKDVTDYTKYLTLKYGGIAKNVEKEIIEKIKIYIKKTISNKRIKEEINDLL
                     LRFHEPAKNDIDDFINYIRLSKIAFQEDELRDEIEKERLYRKFHSPQDTAIP"
     gene            complement(724..2109)
                     /locus_tag="VKL21_10955"
     CDS             complement(724..2109)
                     /locus_tag="VKL21_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMB46327.1"
                     /translation="MMQKKEYDVIIVGAGPAGMFAAYELAGKMTVLVIDMGRDINERE
                     CKMKRRGICTHCDPCDIMCGVGGAGTYSDGTLNLRPDIGGDLAEQTHDPEYAWQLVDY
                     VDKVFLKYGAPDHLFIPKGDEVEQLKRRAASVGARFIEIIQRHIGSDNAPIVIENFEK
                     DMKEKGVEFLLGVKVEDLIIEENTCTGVILKNDIKIKSRFTIIAPGRVGAKWIDEILH
                     KHNIEAKYAPIDVGVRVEVPSIIMDPITYINHDPKFHIQTKTYDDFVRTFCTNEKGFV
                     VKEEYEDFIATNGHSMTNEQSENTNFAFLVRVELTEPIENTVRYGRSIAKLATTIGGG
                     KPVLQRMGDLRRGRRSTEKRLKKNQVINTLKDVTPGDISMALPHRIVTDIREGLEVLN
                     EIIPGVAADSTLLYAPEIKFYSMQVIVDTNMESSVKNLFAAGDGAGLSRDIVNAAATG
                     VIAGRGILKKV"
     gene            complement(2106..>3173)
                     /locus_tag="VKL21_10960"
     CDS             complement(2106..>3173)
                     /locus_tag="VKL21_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HMB46328.1"
                     /translation="VEKKEILKILGGYDLENIVIATVCSHSSLQIFDGARKEGFKTIG
                     ISVGSPPKFYDAFPKAKPDEFFIVKDYKEILSKTQELAAKNAILIPHGSFVEYLGAQN
                     FQSMRLPTYGNRSVLEWESDRDMSRKWLESAGIKMPRQIKNPEDIDSPMMVKYYGAKG
                     GMGFFVVKNYSDFQKRINPAEKFTIQEFVLGTRYYMHYFYSPIKQDGYKLSTGSLELL
                     GIDRRVESNADEIFRIGSPTELAEAGIYPTFVVTGNLPLMIRESLLPKIFEMGERVVE
                     KSIDLFGGMIGPFCLETVVTDSLELKVFEISARIVAGTNLFISGSPYSDLVDENLSMG
                     RRIAREIKVARERNVLPEVIS"
BASE COUNT          793 a          791 c          572 g         1017 t
ORIGIN      
        1 ggtattgcag tatcctgggg gctgtggaat tttctgtaaa gcctctcttt ttcaatctca
       61 tccctcagtt catcttcctg aaaagctatc tttgagagac gaatataatt tataaaatca
      121 tcaatatcgt ttttcgcagg ctcatggaat cttaaaagta aatcgtttat ttcctctttt
      181 atccttttat tgctgattgt cttctttata taaattttaa ttttttctat tatctctttt
      241 tccacattct tcgctatacc accgtatttt agtgtcagat attttgtata atccgtaaca
      301 tctttttcag ttggcaatgt atttttcgcc aggaaagtat ctatttcttt ttccatatca
      361 ctggtaagga catttccgga ttgtttttgt ttcccttcaa gatcaagggc tttgctcctc
      421 aatgtatttc caatatctat agccttattc cttcctgcca ttatactaat atttatttca
      481 atgaggtcta tgaattcttt aaaatcgctt atatttgatt tatcattcag gcttttcttt
      541 atttttattt tatttacaat tccaccagct aattgcccga aatgtatttc aagcgtctga
      601 atgcaatatt tttcaaattc attggactcg gccataataa tcgtttcact atgttattat
      661 acttatgatt atgaaagtat aaaaagataa tgctgggatt aagatatgat caaatctgaa
      721 ggcctaaacc ttcttcaata tccctcttcc cgcaatcacg cctgtggctg cggcatttac
      781 aatatccctt gaaagtccgg ctccatcacc cgccgcaaag aggttcttga cgcttgattc
      841 catgttcgta tccacgatca cctgcattga atagaacttt atctcaggcg catacagaag
      901 cgtggaatcc gctgcaactc caggaattat ttcattgagc acctcaagtc cttcccttat
      961 atcagtcact atgcgatgcg gcagggccat ggaaatatca ccgggagtca catccttcag
     1021 cgtatttatc acctggtttt tcttgagcct cttctctgtg cttcgcctcc cgcgcctcag
     1081 gtctcccatc ctctggagaa ccggctttcc gccgccgata gtcgttgcca gctttgcgat
     1141 agatcttccg tagcgcacgg tattctcgat gggttcagtg agttcaaccc ttacgaggaa
     1201 agcgaaattc gtattctcag attgctcatt cgtcatggaa tggccattcg tggcaatgaa
     1261 atcctcatat tcttccttta ccacaaaacc tttctcattt gtacaaaaag tcctcacaaa
     1321 atcatcatag gtcttggtct ggatatggaa tttaggatca tggtttatgt atgttatcgg
     1381 gtccattatt attgaaggga cttcaaccct cactcctaca tcaataggag catattttgc
     1441 ctcaatattg tgtttatgca ggatctcatc gatccatttc gctcccaccc ttcctggcgc
     1501 tatgatcgta aagcgggatt ttatctttat atcattcttt aatataacac ccgtacaggt
     1561 attttcttcg ataatcaggt cctctacttt cacgccaagc aggaactcaa cacctttctc
     1621 cttcatatcc ttctcaaaat tttcgataac tataggcgca ttatctgacc cgatatgtct
     1681 ctggattatc tcgataaacc gcgcgcctac cgatgccgct ctgcgcttta actgctcgac
     1741 ctcgtcgcct ttaggaataa aaagatgatc aggagcgcca tatttaagga ataccttatc
     1801 cacataatca acaagctgcc atgcatattc cgggtcatgg gtctgctcgg caagatcccc
     1861 cccgatgtca gggcgaagat ttagcgttcc gtcagaatac gttcccgcac ctcccacgcc
     1921 gcacataata tcgcatgggt cgcagtgggt gcatattcca cggcgcttca ttttgcattc
     1981 gcgctcgttt atatcacggc ccatgtcaat gacaagaact gtcattttcc cggcaagttc
     2041 ataagctgcg aacattcctg cgggtcctgc acccacaata atgacatcat attccttttt
     2101 ctgcatcatg atattacttc cggaagcaca ttcctttccc gcgccacttt gatctcgcgc
     2161 gctattcgcc gccccatcga gaggttctca tccacaaggt cggaatacgg tgaaccggaa
     2221 atgaacaggt ttgttcctgc aacaatgcgg gccgatatct caaaaacctt caattcaagt
     2281 gaatctgtta caactgtttc gaggcagaat ggccctatca tacccccgaa cagatcaatc
     2341 gatttctcca ctaccctctc gcccatttca aaaatcttgg gcaggagtga ttctcgtatc
     2401 ataaggggca ggtttcctgt caccacaaaa gtcggataaa tgccggcttc tgccagctct
     2461 gttggtgaac cgatcctgaa gatttcatca gcgtttgatt caacccgcct gtcaatgccg
     2521 agcagttcaa gtgaacctgt acttaactta taaccatcct gcttgatcgg tgaataaaaa
     2581 taatgcatgt aataacgtgt tcccagaacg aattcctgga tggtgaattt ttcagcggga
     2641 ttgatccttt tctggaaatc ggaataattc ttgaccacaa aaaatcccat cccgcctttt
     2701 gcgccataat acttgaccat catgggagaa tcgatatcct ccgggttctt tatctggcgc
     2761 ggcattttta tgcccgcaga ttccagccac ttgcggctca tgtccctgtc tgattcccat
     2821 tcaagcacgc tacggttccc atatgtggga aggcgcattg actggaaatt ttgcgcgccg
     2881 agatattcca caaaagaacc gtgcgggatt aagatcgcgt tttttgctgc aagttcttgt
     2941 gtttttgaaa ggatctcctt ataatctttc acaatgaaaa attcatccgg ttttgccttc
     3001 gggaaagcat cataaaattt tggcggtgac ccgacgctga tcccgatggt cttgaaacct
     3061 tccttccttg ccccatcaaa aatctgcagg ctgctatgtg aacaaacagt agcgataact
     3121 atattctcta aatcataccc acctaagatc ttaagtatct ccttcttttc tac
//
LOCUS       DATGBN010000436         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_112992, whole genome shotgun sequence.
ACCESSION   DATGBN010000436 DATGBN010000000
VERSION     DATGBN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112992"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VKL21_10965"
     CDS             <1..219
                     /locus_tag="VKL21_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46329.1"
                     /translation="LNSGDLIEISIKAETITDIGTTPPNTDPNKGIILAPRKTFWLSL
                     NQELGQAVNLEIATPNSFGVNRFVPLYP"
     gene            235..633
                     /locus_tag="VKL21_10970"
     CDS             235..633
                     /locus_tag="VKL21_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46330.1"
                     /translation="MGFSTSAVVVIFIASIMYMATMFYPLADMSYQQLQEAKKNSNEL
                     WNEKLNTKIVITKWEGNIITVSNNGSIPLNSSKINVIINGDFISSPSYTVNPTGVWPP
                     RTSIDVNIGTGSGKVKIITANGAADYYSLP"
     gene            643..1095
                     /locus_tag="VKL21_10975"
     CDS             643..1095
                     /locus_tag="VKL21_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein G"
                     /protein_id="HMB46331.1"
                     /translation="MGAGASATQMIFFITSVIIALSVVGAMFLNIQSISSAAIVGSKT
                     LAEQLKTDITIINDPESIPHPGGSNYSFYIKNTGKEGLGIQYISVIIDGTIVTDGNLN
                     KTILEGGQMWLSGDVLQIDAATSLASGSHNLMIITGNGIEDTLLFRTP"
     gene            1098..>1764
                     /locus_tag="VKL21_10980"
     CDS             1098..>1764
                     /locus_tag="VKL21_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HMB46332.1"
                     /translation="MASYNFVLDRDELNQMLGGGFPKGSLIMIDGPSGSGKSALCQRF
                     AYGLLDNKNTVTYISTQLTTKNFISQMKSLDYPIGIRLLKGELLFIPVFPLLKESKPR
                     IDFIQRLMGAEGLYEKNIIVIDTLSALIRYGTDAAKSLELISFFKKISGLQKTIIITM
                     DSAELPTHVVSEFKDASEILITLKMRQMGTEMLRTMMINKYSRPEIQVGAMIGFKVEA
                     RSGV"
BASE COUNT          584 a          349 c          367 g          464 t
ORIGIN      
        1 cttaatagtg gagatttaat agaaatctca ataaaagccg aaactataac agatattggc
       61 actactccgc caaatacaga tccaaacaaa ggtataatat tagctccaag aaaaaccttc
      121 tggttatctt tgaaccagga actcggtcag gccgtaaatc ttgaaatagc aacacccaac
      181 tcgttcggag taaacagatt cgtcccacta tatccgtaac agcggggaga acatatgggt
      241 ttttcaacct ccgctgttgt ggttattttc atagcgtcca taatgtatat ggcgacaatg
      301 ttctatccgc tggctgatat gtcatatcag cagttacaag aggcaaagaa aaattcaaat
      361 gaattatgga atgaaaaatt gaatacaaaa atagtaatca caaaatggga ggggaacatc
      421 atcaccgtct ctaacaatgg ctcaattcca ctgaattcca gcaagatcaa tgtaattata
      481 aacggggatt ttatatcaag cccctcttat acggtaaatc ccactggggt atggcccccc
      541 aggacatcta ttgatgtaaa tatcggaact ggaagcggca aggtaaagat aatcaccgca
      601 aatggcgcag ctgattatta ttcgctgccc taggagaaaa aaatgggagc aggcgcatca
      661 gcaacccaga tgatcttttt catcacatct gtaataattg cgttaagtgt agtaggagca
      721 atgttcctga atatccaatc catatcatcg gctgcaattg tcgggagtaa gacattagct
      781 gaacagttaa agacagatat caccatcata aacgatcccg aatccatacc tcatcccggc
      841 ggtagtaatt attcatttta tataaaaaat acggggaagg aaggtcttgg catacaatat
      901 atttcagtca ttatagacgg gactattgtt actgacggca atcttaataa aacaatcctt
      961 gagggcggtc agatgtggct ttcaggagat gtcctgcaaa tagatgcggc aacttccctt
     1021 gcgtcaggca gtcataatct catgatcata acaggcaatg gaattgaaga tacgctttta
     1081 ttcaggacac cttgaccatg gcatcatata actttgtgct tgacagggac gaattgaacc
     1141 agatgcttgg cggcgggttc ccgaaaggtt ccctgataat gatagacggt cccagcggaa
     1201 gtggaaaaag cgcattatgc cagagatttg cttacggtct tcttgataat aaaaatacag
     1261 ttacatacat atcaactcaa ttgaccacaa aaaatttcat cagccagatg aaatcacttg
     1321 attaccccat cggcatcagg ttgctaaaag gagaattatt gttcatcccg gtttttccat
     1381 tgttaaaaga atcaaaaccc cggatcgatt ttatccagcg tctaatgggc gcagaaggac
     1441 tttatgagaa aaatattatt gtaatagata ctctttcggc gcttatccga tacgggaccg
     1501 atgcagcgaa aagtcttgag ctcatttcat tttttaaaaa aatatcgggt ttgcagaaaa
     1561 caattatcat tactatggac agcgccgagt tgccaactca cgtagtttct gaattcaaag
     1621 atgcttccga gatcctgata acattaaaaa tgcggcagat gggaactgaa atgttgcgga
     1681 caatgatgat aaataaatat tcacggcctg aaatacaggt tggggcaatg atcggattta
     1741 aggtagaagc aagaagtggc gtca
//
LOCUS       DATGBN010000437         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_229226, whole genome shotgun sequence.
ACCESSION   DATGBN010000437 DATGBN010000000
VERSION     DATGBN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229226"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VKL21_10985"
     CDS             <1..782
                     /locus_tag="VKL21_10985"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HMB46333.1"
                     /translation="QLLVNTRPTAVSLPNAVRAVMRYEGDSVEEAKASIKELADGFIL
                     NSENAVRKIGEIGARRVRDGDTIMTHCNSSAAISIMAAAHDDGKNIRVIATESRPRWQ
                     GHLTVKQLDEKGIKTSLIVDSAVRYFMKEADLVVMGADAVTVNGSVINKIGTSQLALA
                     AHEARKNVIIAAETYKFSPKSLLGELLEIEERSSSEVLPDEELKEFSNVSVKNPAFDV
                     TPREYIDLICTEVGAIPPEMAYIIIRECLGWELEDIDSWEN"
     gene            848..1363
                     /locus_tag="VKL21_10990"
     CDS             848..1363
                     /locus_tag="VKL21_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46334.1"
                     /translation="MNSTEIVLFTIIVFLFIIAIFMFVLLRKSFERKKLQELKKFEYM
                     DIVNITPEFKFETPGFNTPDIPSIDEIMKPVTYAAETKESKEKIPSENKEIKNHDEEL
                     KEILIAEPKTIEPLADDNVPQAIAEDYSGLKLEDTLIDIEDEKKERTKTVAKRPETKV
                     AEKDHLKKREK"
     gene            complement(1508..1885)
                     /locus_tag="VKL21_10995"
     CDS             complement(1508..1885)
                     /locus_tag="VKL21_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46335.1"
                     /translation="MSTINILLQLTTIVLALIGAYLAANTSDLMRKKMDINLLRAKAF
                     LHDSFIKDNWKLLFIVCFLFLINAAIYFDEMLGLFIKEDNSHLLQEIILLGILTCSVI
                     SQYKWFRLVRPVKCDGFTSGKRN"
     gene            2042..>2438
                     /locus_tag="VKL21_11000"
     CDS             2042..>2438
                     /locus_tag="VKL21_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46336.1"
                     /translation="MTTELRNALYGRSSVFVGSLDTPTERIVYHILYETVRTGKTVIW
                     ICLRDTPNTIFSKFSSYDLPMEGFEENLWFVDSTIIGDTTITPRTYRCPSLDYACIIM
                     EVVKLLKKYPTSLVMFDNIGILAVLDRVNA"
BASE COUNT          820 a          424 c          506 g          688 t
ORIGIN      
        1 cccaacttct tgtaaataca cgccccacgg cagtatcatt acccaatgcg gtccgcgctg
       61 tgatgagata tgaaggagat agcgttgagg aggcgaaagc cagtatcaag gaactggcag
      121 atggatttat cctgaattcc gagaacgcag tgagaaagat aggcgagata ggcgcgcgaa
      181 gagtgcgcga tggtgatact atcatgacac actgtaactc gtctgccgca atttcaataa
      241 tggctgccgc gcatgatgat ggaaaaaata tccgtgttat agcaacggaa tcccgtccaa
      301 gatggcaggg gcatctgacg gtaaagcagc ttgatgaaaa aggaataaaa acatcattaa
      361 tagtggactc agcggttcga tattttatga aagaagctga ccttgtagta atgggtgctg
      421 atgccgttac tgtaaatggc tctgttatca ataaaatcgg gacttcccag cttgcccttg
      481 ccgcacatga ggcgaggaag aacgtaataa ttgcagccga gacctataaa ttcagcccga
      541 aatcattgtt gggcgagctt cttgaaatag aagaacgaag cagttccgaa gttttgccgg
      601 atgaagaatt gaaggaattc tcaaatgtat ctgtaaaaaa cccggctttt gatgtaactc
      661 cgcgcgaata tatcgacctt atttgcacgg aagtgggggc catccctcct gaaatggcgt
      721 atattattat cagggaatgc ctgggatggg aattggagga tatagattca tgggaaaatt
      781 aggatatgtt cttttgtaat caaaagaatt aaatatcata attataatta tataataagg
      841 aaacatcatg aattctactg aaatcgtgct tttcactatt attgtttttc tattcattat
      901 agctattttc atgtttgttt tgctcagaaa atcgtttgaa agaaagaaac tgcaagaact
      961 aaaaaaattt gaatatatgg atattgttaa tataaccccg gaattcaaat ttgaaacccc
     1021 aggatttaat acgccggata ttccatctat agatgaaata atgaaacccg taacgtatgc
     1081 tgcagaaaca aaagaaagta aggaaaaaat tccatccgaa aataaagaga ttaaaaacca
     1141 tgatgaagaa ttgaaggaaa ttctgatagc ggaacccaaa accatagagc ctctcgcaga
     1201 tgacaatgta ccacaggcta ttgccgaaga ttattcaggt cttaagcttg aagacacgtt
     1261 gattgatatc gaagacgaaa aaaaggaacg aacgaaaact gttgcgaaga gaccagagac
     1321 gaaggtcgct gaaaaagatc atctgaaaaa aagagaaaag taggggtgtg ttttccaata
     1381 gtcagaaaag cagaggctcc ggtaatgtat ctcggagtga ccgcaaatgg gaagcagaaa
     1441 gaatacaatt ttgatctgga atcatatatt tcttagcttc aggaaatgtc aaaaacttta
     1501 tgacggatta atttcttttt ccagacgtaa atccatcaca ttttaccggt cttaccaatc
     1561 taaaccattt gtactgtgat attacactgc atgttaagat cccaagaagg ataatttcct
     1621 gcagaagatg actattatct tctttaataa ataatcccag catttcatca aaatatattg
     1681 ccgcatttat caggaataag aaacatacga taaataataa cttccagttg tccttgatga
     1741 aagaatcatg aaggaaagcc tttgccctga gaaggtttat atccattttt tttctcatca
     1801 aatcgcttgt attcgctgca agatatgctc ctataagcgc caaaactatt gttgtaagtt
     1861 gtaataaaat atttattgtc gacatattta gtataccaca aatttgttat gatataccat
     1921 ataataatgt gttatatata tacaaatttg tgataattat gtgaaattct gttatcattg
     1981 gacacaacag gaatgtttaa tgctgttcat gttctaaaat tggaatatta tgtcgaagga
     2041 tatgactact gaattaagaa atgctctata tggaaggagc agtgtttttg tgggttctct
     2101 ggataccccc acagagcgca tagtgtacca tatattgtat gaaactgtcc ggacgggcaa
     2161 aaccgttata tggatatgtc tgagggacac gccaaataca atattttcca agttctcctc
     2221 ttatgatcta cccatggaag gatttgagga aaatttatgg tttgtggatt ccactatcat
     2281 cggggatact accataaccc cacggactta ccggtgccca tccctggact atgcatgcat
     2341 aatcatggaa gtagtaaaat tgttgaaaaa atatcccaca tctctggtga tgtttgacaa
     2401 tataggtatt cttgcggttc ttgatcgagt gaatgcaa
//
LOCUS       DATGBN010000438        10101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_539624, whole genome shotgun sequence.
ACCESSION   DATGBN010000438 DATGBN010000000
VERSION     DATGBN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539624"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1371)
                     /locus_tag="VKL21_11005"
     CDS             complement(172..1371)
                     /locus_tag="VKL21_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HMB46337.1"
                     /translation="MTKVELAAGVYWVGAVDWNLRDFHGYTTLRGATYNAYLIVDEKI
                     ALVDTVKHGFSGEMLNRIREIIDPAKIDYVVSNHVEMDHSGSLPDIMRIAPNAQVICT
                     EKGKEGLIRHFKGKWNFRTVKTGDELKIGKKTLFFINASMLHWPDNMFTYIKEDKILL
                     SNDGFGQHLASSFRFDEDVCSLAGYDCDTVMEEAAKYYANILMPFGGIILKKIEEVQK
                     LGIEIKMIAPSHGMIWTNPAKIIDAYLKWAKGESKQKVLVIYDTMWSSTQTMAEEILK
                     GASDSGVEVSLFHLRKTEWSEIVKEVLESKAILIGSPTLNNGMFPTVGGFLTYLMGLR
                     PKNKLWATFGSYGWAGGAVKAVNEKLKLGGYEPVESIEVNFRPDEQDMEKCYSLGQKI
                     ASMVKAS"
     gene            1574..1729
                     /locus_tag="VKL21_11010"
     CDS             1574..1729
                     /locus_tag="VKL21_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HMB46338.1"
                     /translation="MNKYSCTVCGYVYDPEVGDSPKIKPGTAFEDLPDDWVCPQCGVG
                     KEMFEKM"
     gene            1871..2761
                     /locus_tag="VKL21_11015"
     CDS             1871..2761
                     /locus_tag="VKL21_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB46339.1"
                     /translation="MKQERWGNIGSKFAHLTSVHPCYNEKAHFTTARIHLPVAPRCNI
                     QCNYCIRKLDKCEHRPGVASSILNPREALVRVDKYSREMQNLKVVGIAGPGEALANED
                     TFETMRLVHEKYPELIKCVASNGLLLSERVDELVKAGVTSVTVTINAVDPEVGAKIYS
                     FVRYHDKTYKGVEAAKLLIEKQFEGIKKASEAGLNVKVNTVLIPEINFDQIKKVAKKA
                     AENGAIIMNIIPMIPLHNFEKERPPECDELTMARTIAEEFLPQFRLCRQCRADAVGVP
                     GAEPCGSPVQQRTSSEYYHA"
     gene            2800..2958
                     /locus_tag="VKL21_11020"
     CDS             2800..2958
                     /locus_tag="VKL21_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46340.1"
                     /translation="MCFAESSVSVEIEEGALVTDQYQCSDCNSNFRGIGKKVRCPTCE
                     SVNVKKIC"
     gene            complement(3041..3838)
                     /gene="larE"
                     /locus_tag="VKL21_11025"
     CDS             complement(3041..3838)
                     /gene="larE"
                     /locus_tag="VKL21_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HMB46341.1"
                     /translation="MSLDEKIQSILAALKERGSVLIAFSGGVDSSVIAALAFRSLGKQ
                     AIAVTADSQTFAPGELECAKAVATEIGISHIVINYDELGEPGFAKNPKDRCYHCKKGL
                     IRELKKIAAEHDIGTIVEGTNISDIKGYRPGHRAVIEEGVYNPFVEFKVTKEEIRKIA
                     RKLGLSVAQKPSMACLSSRFPYGQAITADGLKRVGEAESYLCALGFSALRVRDHAGIA
                     RIEIMPGEMVRFLEMRDAVSSEFKRLGFSYVTLDLMGLRSGSMDEVI"
     gene            complement(3926..4309)
                     /locus_tag="VKL21_11030"
     CDS             complement(3926..4309)
                     /locus_tag="VKL21_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46342.1"
                     /translation="MKKIIREETIEESFEHGNGTATVTKKKIKAIQEGEKIIVVIEED
                     GNESVFCNYLKDEYEVMVFFNSIRLDEDIMKNEDFGTNYRYGENPKSTTMCVYFTLPY
                     GEIFSDGLWLNEERDELYKNRDDAK"
     gene            complement(4519..5991)
                     /locus_tag="VKL21_11035"
     CDS             complement(4519..5991)
                     /locus_tag="VKL21_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMB46343.1"
                     /translation="MIKSKTITMIKIALATCSLLLIYPVLAFADLNTNLAENPSFESG
                     ISIPMNWTFISQNGNTPIWDDVFYSGSKSIKISISGTTDNKSGYPQSDLIPAESLTTY
                     TVSVWVKTENAGGKNTPAVRVVELDANKNWLSQTNLPVFGRGTNDWEQKRMYFQTGSN
                     TSYIYIYANIWNGYGTFWVDDVVLGLKDTPILTPVVTATPAVIPGPAVRATPTPESGS
                     TIAYLGDARPSKFGNKGITDLTDDMNQIIPQSPTGKVDAIFMIGDMDHVSQTVQAYKA
                     SKVNTIPIYFVVGNHEIDNGDMGYLRSLPIPTTFKINPAPPGTDKTAFSVDIGDIHVV
                     DMNIYWDGKNNDAYWKYGGSDGGWIAPELYNWVNQDLSSTSSWKVVLGHEPMYPKSRH
                     VGDSLDADSTNRDNFEKLLVSQGVSLFVAAHTHYAAVNLHDSVYHVDAGISGAKTVDG
                     EDPYASITYTVSTPNDLTLTWKHENPTWSNPKVTTYTISK"
     gene            complement(6695..7105)
                     /locus_tag="VKL21_11040"
     CDS             complement(6695..7105)
                     /locus_tag="VKL21_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HMB46344.1"
                     /translation="MASELIELLNKAIAREIGVSVQYMWQHVMAIGMKSPEVKDIFED
                     IAIEEMKHAEKIAERLFYLGGIPTVKPTPIIVGGSLENMVEKDLEAENESIELYKEII
                     DLAAEKEDSTTRLLFEEILTEEEEHKHTFTILLK"
     gene            complement(7154..8146)
                     /gene="purM"
                     /locus_tag="VKL21_11045"
     CDS             complement(7154..8146)
                     /gene="purM"
                     /locus_tag="VKL21_11045"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HMB46345.1"
                     /translation="MALTYAKAGVDIDNENRAIAALAKQLTYKRKGIGAPLTDVGHYA
                     GLIDFGEYALALTTDGVGSKVLIANEMKKWGTIGIDCIAMNVNDLLAMGIEPLAFVDY
                     IAIHEPNHEIMGQIGEGLAKGARISRMTIVGGETATLPDIINGFDLAGTCLGMVKKDK
                     VITGERIEIGDAIVGIPSSGVHSNGYSLVRRIIKDAGYSYHDPFPYNEDTTIGDELLT
                     PTRIYMEILDAVPDFDIHGLAHITGSGLMKLHRTTKYGFDINDPLEPQPIFKFLQEEG
                     EVEDLEMYKTFNMGMGFVVVIPEAKAKSAAKMLGGKVIGEIVDEGIKVKGLEIK"
     gene            8528..8959
                     /locus_tag="VKL21_11050"
     CDS             8528..8959
                     /locus_tag="VKL21_11050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMB46346.1"
                     /translation="MPNRTAILETAKGTIKFELYETETPLTTKNFIDLTNKGFYKGLI
                     FHRVEPGFVIQGGDPKGNGTGGSGKNIPLEITPTLTHKKGAVGMARSQEPNSANSQFY
                     ICLEDSKFLDKNYAVFGQVTEGQDVAQKIKKGDKIIKATIQ"
     gene            8970..9527
                     /gene="afpA"
                     /locus_tag="VKL21_11055"
     CDS             8970..9527
                     /gene="afpA"
                     /locus_tag="VKL21_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeoflavoprotein AfpA"
                     /protein_id="HMB46347.1"
                     /translation="MTIRIAWGITGCGDYLKESLDIMKELTKEHDLDVRVFLSRAGEM
                     VVKWYKLFNELKTSFPKTYSEKSPNVPFLVGDLQLGKYDFLIIMPSTSNTVAKIAAGI
                     SDTLLSNAASQAMKANVPVYIFPADQKKGDIVTDLPGGKKLTLTMRDVDLEAVDRLRK
                     MKGITVLGHPDEIRTIVKKHIENKK"
     gene            complement(9529..>10101)
                     /locus_tag="VKL21_11060"
     CDS             complement(9529..>10101)
                     /locus_tag="VKL21_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HMB46348.1"
                     /translation="RVFELLFQNHRSNSMNLIILSDTHIKQGHSLLSRLPEELISIIK
                     SSDLIIHAGDFETLTCYNELEALGKLAAVHGDTDSPELMKFLPERKVINIEEIKIGVI
                     HKGQLTSENTDGLRYLAKEMGVDVLIFGHFHHPIAHKSEVLLLSPGSATVPGIAEPSA
                     IWLEIKNKTVTGKIIRCKDNSCTYFEFEKH"
BASE COUNT         2922 a         2136 c         1940 g         3103 t
ORIGIN      
        1 ctacgctcca atattatata tcccagatat gccatttgaa aaatgtgacc actactcagg
       61 tgataaatgc actgcttcag atcttgagcc atgcgatttc aacggagaag atttcgagaa
      121 atgccttcga tacaggctta atttattaag accacagcta atgcagttgc gttagcttgc
      181 ttttaccatc gaagctattt tctgccccag agaatagcat ttctccatat cctgctcatc
      241 cggcctgaaa ttcacttcta tcgactcgac aggctcatat ccgcccaatt ttaatttctc
      301 attgactgcc ttgactgccc cgccggccca gccataagaa ccaaaggtcg cccagagctt
      361 gttcttagga cgcagcccca tcagatacgt caaaaatccc cctacagttg ggaacatgcc
      421 attattcaaa gtgggtgagc cgataagaat cgcctttgat tccagaactt ctttgactat
      481 ttcactccat tcagtctttc tcaggtgaaa tagggatacc tcgacaccag aatccgaagc
      541 tccttttaag atttcctcgg ccatcgtttg ggtactgctc cacatggtat cataaatgac
      601 aagaactttc tgctttgact cgccctttgc ccattttaaa taagcatcga tgatctttgc
      661 gggatttgtc catatcattc cgtgggaagg agcgatcatc tttatttcga tgccaagttt
      721 ctggacctcc tcgatctttt tgaggattat tccaccaaag ggcatgagga tattggcata
      781 atatttcgca gcctcttcca taacggtatc gcaatcatac ccggcaagac tgcaaacatc
      841 ttcatcaaaa cggaatgaag aggcaaggtg ctggccgaaa ccatcatttg aaagaagtat
      901 tttgtcctct tttatgtatg tgaacatatt gtccggccag tgaagcatcg acgcattaat
      961 gaagaaaagt gtcttcttgc caattttcaa ttcatccccg gttttcaccg tcctgaaatt
     1021 ccacttaccc ttgaaatgcc tgatcagccc ttcctttcct ttttcggtgc aaattacctg
     1081 tgcattcggg gcaattctca taatatcggg aagagaacct gaatgatcca tttccacatg
     1141 gtttgaaacc acataatcaa tttttgcggg atctattatc tcccgtatcc tgtttagcat
     1201 ctcacccgaa aaaccatgtt ttacggtatc aacaagcgca atcttttcat caacgataag
     1261 atatgcgttg tatgttgctc ccctcaaagt ggtgtatcca tgaaaatccc gaaggttcca
     1321 gtccacagct cccacccaat aaactcctgc tgccaattcg acttttgtca tttatattct
     1381 cctgcgatca cattactcct aaaatatcgc acattgtctc acaagtatct ataaacaaca
     1441 catttaattc tttctgctca aaatgtattc ctgttaaaaa aacacctggt ttaacatttg
     1501 ttttcttctt gatggtaatc cttatcttat atgccagaat taaaagaatt atttaataga
     1561 ccagggagga aacatgaata aatacagttg tacagtgtgc ggatatgttt acgatccgga
     1621 agtaggagat tccccaaaga tcaagcctgg aactgcattt gaggatttgc ctgatgactg
     1681 ggtatgcccc cagtgcggag ttggcaagga aatgtttgaa aaaatgtaaa tatgatttaa
     1741 aagaaaaagt gtataaagcc acaattggga aaatacctat atagtatcaa ttctaatagt
     1801 cagttcgtat aattacgaac tgaaaagaga gagtcagttc gtataattac gaactgaaaa
     1861 gagagaaaaa atgaaacaag aacgctgggg caacataggc tcaaaattcg cgcatcttac
     1921 aagcgtacac ccgtgttata atgaaaaagc gcatttcacg accgcaagga ttcacctgcc
     1981 ggtggcgcct cgctgtaata tccagtgcaa ttattgtatt cgtaaactgg ataaatgtga
     2041 acacaggcca ggcgtggcaa gtagtatact caatccccgc gaagcacttg taagggttga
     2101 taaatactcc agggaaatgc aaaatctcaa ggtcgttggt atcgcaggac ccggcgaagc
     2161 gcttgcgaat gaagatacct ttgaaaccat gcggcttgtg catgaaaaat atcctgaact
     2221 tataaaatgc gtggcaagca acgggctttt gctatcagaa cgagtggatg aacttgtcaa
     2281 agcaggtgta acaagcgtta ccgtaacgat aaatgcggtt gatcctgaag tcggcgcaaa
     2341 gatatattcg ttcgttcgtt atcatgataa gacctataaa ggtgttgaag ctgcaaaact
     2401 cctgatcgag aaacagttcg aggggataaa gaaagcaagc gaagccggac tgaatgtcaa
     2461 agtcaacaca gtgcttattc ctgagatcaa ttttgaccag ataaagaaag tcgcaaaaaa
     2521 ggcggcagag aatggcgcaa taataatgaa tatcatcccc atgataccgc ttcataattt
     2581 tgaaaaggag agacccccgg aatgcgacga acttacgatg gcaagaacca ttgcagaaga
     2641 attcctgccc cagttcaggc tgtgcaggca gtgccgcgca gatgctgttg gcgtgcccgg
     2701 cgcggaaccc tgcggttcac ccgtgcagca gcgcacaagc agcgaatatt atcatgctta
     2761 aaattataaa ataattaaat atgcaagaga ggtgaaaaaa tgtgctttgc agaatcatcg
     2821 gtcagcgtgg aaattgagga gggagctctg gtcacagatc agtatcaatg cagtgattgt
     2881 aactcaaatt tccgcgggat cgggaaaaag gtaagatgcc caacgtgtga gtcggtcaat
     2941 gtgaagaaga tatgttaaac tagccaattt tctttaataa gaaatttttg tctttggagg
     3001 ctatgctgaa acattcttta gcttcggcaa cagatggtaa tcatatcacc tcatccatgc
     3061 tgccgctgcg caaacccatg aggtcaagcg ttacatacga aaacccaagc ctcttaaact
     3121 cagaagaaac cgcatcgcgc atttccagaa aacgaaccat ctcgcctggc atgatctcga
     3181 tgcgggcaat tcctgcatgg tcgcgtacac gcaatgcact gaatccgaga gcacaaagat
     3241 aactctcggc ttctccgact cgctttaacc catcagccgt gatagcctgc ccatagggga
     3301 accgcgaaga aaggcatgcc attgatggtt tttgtgcaac tgataagcca agtttccggg
     3361 ctattttcct gatttcctct tttgtgacct taaattccac gaaaggatta tagaccccct
     3421 cttcaatgac tgccctgtgg cctggcctgt aacctttaat atccgaaata tttgttcctt
     3481 cgacaatcgt tcctatatca tgttctgctg cgattttctt tagctccctt atcagtccct
     3541 ttttgcaatg atagcatcta tctttggggt tttttgcaaa acccggctct ccaagttcat
     3601 cgtagtttat cacaatatgc gaaataccga tctctgttgc tactgctttt gcgcattcaa
     3661 gctcaccggg cgcaaatgtc tgtgaatccg ccgtgacagc gatagcctgt tttccaagag
     3721 atcgaaatgc aagcgctgca atcacggagc tgtcaactcc ccctgaaaat gcaataagaa
     3781 cactgcctct ttctttaaga gccgcaagaa tagattgtat tttttcatca agggacattg
     3841 caatgagaac acttttttaa atcggacatt acttagaatc ggagttatat aaatccttct
     3901 ttttaatatt cataaattgt acatatcatt ttgcatcatc cctgttttta tatagctcat
     3961 ctctttcttc attaagccac aatccatcag aaaaaatttc tccataggga agggtgaaat
     4021 aaacacacat agttgttgat ttaggatttt caccataacg ataattggtt ccaaaatcct
     4081 catttttcat tatatcttca tctagtctaa tgctattgaa gaaaaccatg acttcatact
     4141 catctttcag gtaattacaa aaaaccgatt catttccatc ttcttcaatt accactatta
     4201 ttttttctcc ttcttgtatg gcttttatct tttttttcgt tacagtagcg gtaccgtttc
     4261 catgttcaaa actttcttca attgtttctt ctctgattat ctttttcatg attttatttc
     4321 ctttatgtaa gtttgttcat acacctttac gttaaataac atatgatgac ggtttcgatt
     4381 aagaattttt cataggaatg agatttcttg ggaggattat taactcgaac tgttttaaaa
     4441 aaagaaattc gatcaaacag aggtgccccc gctttctaag cgggagtgct gaatttgttg
     4501 aattgtagtc ctgtttcttt atttactaat tgtatatgtt gttacttttg gattactcca
     4561 tgttggattc tcatgtttcc aggttaatgt taaatcatta ggagtagaca ctgtataggt
     4621 aattgatgca tacgggtctt ctccatctac cgtctttgca cctgagatac ctgcatcaac
     4681 atgatatacc gaatcatgaa ggtttacagc tgcataatgg gtatgcgctg caacaaataa
     4741 agatacacct tgagatacta ataatttctc aaaattatct cgatttgttg aatctgcatc
     4801 taatgaatca cctacatgtc gtgacttagg atacatgggt tcatggccta aaacgacctt
     4861 ccagcttgac gttgatgaca aatcttgatt aacccaatta tataattctg gtgcaatcca
     4921 tccaccatca ctaccgccat atttccaata tgcatcattg ttctttccat cccaatatat
     4981 gttcatatct actacatgga tatcaccgat atctacggaa aatgctgttt tgtctgtacc
     5041 cggtggtgca ggattgatct taaaagttgt tggtatgggt agacttctaa ggtaccccat
     5101 atcaccatta tcaatttcat ggttacctac tacaaagtaa atcggtattg tgtttacttt
     5161 agatgcttta tatgcttgaa ccgtttgaga aacatgatcc atatctccaa tcatgaatat
     5221 ggcatccact tttccggttg gtgactgcgg aattatctga ttcatgtcat cagttaaatc
     5281 agtaatacct ttgttgccga atttagatgg tcttgcatca ccaagataag ctattgttga
     5341 acctgattct ggagttggcg ttgctcttac agcaggccct ggtattacag caggtgttgc
     5401 tgttacaaca ggtgttagta ttggagtgtc tttcaatcct aatactacat catccaccca
     5461 gaaagttcca tagccattcc agatattggc atatatgtaa atataggaag tattcgaacc
     5521 agtttgaaaa tacattcttt tttgttccca gtcatttgta cctctgccga atactggaag
     5581 gttagtctgg ctcagccaat tcttgtttgc atcaagctcg actactctga ctgctggggt
     5641 attcttgccc cctgcatttt ctgttttgac ccatactgag actgtataag ttgtaagaga
     5701 ttctgctgga attaaatcag attgtggata tccggattta ttatctgtcg ttccagagat
     5761 gcttattttt attgattttg agccgctgta aaatacatca tcccatatcg gggtgttacc
     5821 attctggcta ataaatgtcc agttcatagg aattgatatg ccgctttcaa agctgggatt
     5881 ttctgcaagg tttgtattta aatctgcaaa tgccaaaacg ggataaatta gcagcagact
     5941 acaagttgca agtgcaattt taatcatagt tatcgttttt gattttatca ttttattttc
     6001 accaattata ttttattcac tatatctact ataggaatgg gatttaatga aataaaaaga
     6061 ctctgatggt tattagaagt ttttctgagt ttttaaagta cattatagtt tattttaatt
     6121 ttttattgca taattattca ttttttttta atacgataaa aatatagctt ttttaatgaa
     6181 ttgaaagctt tatttacgat ggtttgcttc tcttcctgac tcgatttctg aactggattt
     6241 tttggcttgc tcgatttatg catttagtag aatgcattct cactcatttc agaactttcc
     6301 caattttcac ttattaaaaa gttacctcat aaggtcagtt gacacttaac actaatgcaa
     6361 tatccggatt tcttctcaga actgtttcca tatgaaatga aggtgaaata gttaacttat
     6421 ttatgaagac tgatattatg gtgctaataa agatatctat tattaatgta taattttgat
     6481 tcgatagcta atgactgatt taccacgtca tctttccatc ataataatga aaatcattat
     6541 taaatttttc catgagaaat aaaggggtta aaataataat aaaaatgcaa aaatgattac
     6601 aaacactgac tatttaaaaa aaaatgttct ttaagaaaaa taatcctcgt ggggtcaggg
     6661 ggtagaaaag gtccctccga aggggagggg tagttcactt caacaggata gtaaatgtat
     6721 gtttatgttc ttcctcttct gtgaggatct cttcaaaaag caatcttgtg gtcgagtctt
     6781 ctttttctgc agcaagatcg atgatttcct tatatagttc aatagattca ttttctgctt
     6841 ccaggtcttt ctcaaccata ttttcaagtg aaccaccgac aatgatcgga gtgggtttga
     6901 cagttgggat cccgccaaga taaaaaagcc tctcagcgat cttctcagcg tgcttcattt
     6961 cttcaatggc aatatcttca aaaatatcct taacttcagg gcttttcatc cctattgcca
     7021 ttacatgctg ccacatatat tggacactta cacctatttc cctggctatc gctttattaa
     7081 gtaattctat taattcactc gccataattg atctattcta tatttatttt gttcttattt
     7141 aaggtttgac acttcacttt atctccaacc ctttaacctt aataccttca tctactattt
     7201 cccctattac ctttcccccc aacatctttg cggcactttt tgcctttgcc tccgggatca
     7261 ccacaacaaa acccataccc atattgaatg tcttgtacat ttccaggtct tcaacttctc
     7321 cttcttcctg caggaacttg aatatcggct ggggttccaa cgggtcgttg atgtcaaaac
     7381 catattttgt agtgcggtga agtttcataa gcccgcttcc ggtaatatga gcaagcccat
     7441 gaatgtcgaa atcaggtact gcatcaagta tttccatata tatcctggtg ggggtcaaca
     7501 attcatcccc gatagtcgta tcctcattat atggaaaagg atcatgatat gaatagcctg
     7561 cgtcttttat gatcctcctg acaagggaat atccattgct gtgtacgccg cttgatggta
     7621 tacctacaat ggcatcccct atttctatcc tctcgcctgt gataacctta tcttttttca
     7681 ccatacccag acatgttccg gcaaggtcga aaccattaat gatatcaggc agtgttgctg
     7741 tttccccccc cacgatcgtc atcctcgata tcctggcgcc ttttgcaagc ccttccccga
     7801 tttgtcccat tatctcatga ttgggttcat gaatagcgat gtaatccaca aatgcaagag
     7861 gttcaattcc catagccaga aggtcgttca cattcatggc gatacaatcg atcccaattg
     7921 taccccattt tttcatctcg tttgcgatca ggacctttga accaacacca tcggtagtta
     7981 aagcaagagc atattcgcca aagtcaataa gacctgcata gtgaccaacg tccgttaaag
     8041 gggcgccaat gcctttgcgt ttataggtaa gttgttttgc cagggcagct atcgcccggt
     8101 tctcattgtc gatatcaacg cctgcttttg cataagttaa cgccatacac gcttcaataa
     8161 aagtgattgc ataaagattt tatgattgga tagagcaggc gttggatagg tgtgtgtgtg
     8221 ggagtgacat aaaaaaattg cctgctcata cttatgatac tcataatagt atatatattt
     8281 ccagtcaaaa aaagtgtaaa aggtaaataa tgataatggt ccttttctgg aaaagtcgtg
     8341 ataaaaaggc gcatctgaat atatattatt tggatagagc aggcgttgga tagttgtgtg
     8401 tgggagtgac ataaaagaat gcctgctcat acttatcata attacgatag tatttaattg
     8461 ttttggttat caaaggacat tcaaaaatca cttataccat gaagtcaact aagttacaac
     8521 gataattatg ccaaacagaa ctgcaatctt agaaaccgca aaaggaacga tcaaattcga
     8581 actttacgaa accgaaaccc cactcaccac aaagaacttc atcgacctca caaataaggg
     8641 cttttataaa ggcctcatct tccaccgcgt cgaaccggga ttcgttatac agggcgggga
     8701 cccaaaaggg aatggaacgg gcggatcggg aaagaacatt cctcttgaga taactccgac
     8761 actgactcat aaaaagggag cagtaggcat ggcgcgctcg caggaaccca acagtgcaaa
     8821 ctcgcagttc tatatatgcc ttgaggattc aaaattcctc gataagaact atgccgtttt
     8881 cggacaggtc acagaaggcc aggatgtagc gcagaagatc aagaagggcg ataagataat
     8941 aaaagctacg atccaatgat gtttaatata tgactatcag aatcgcatgg gggatcacag
     9001 gctgcggcga ttatctcaaa gaaagcctgg atataatgaa agagcttacg aaagaacatg
     9061 atcttgatgt cagggtgttt ctttcccggg caggcgagat ggtcgtaaaa tggtacaagc
     9121 tctttaatga gctaaaaaca agtttcccca aaacatattc ggaaaaatcc cctaatgtgc
     9181 cgttccttgt aggggatttg cagcttggaa aatatgattt tcttattatt atgccttcga
     9241 catccaacac cgtggcaaaa atcgctgcgg gtatttcgga caccctgctt tcaaatgctg
     9301 cgagccaggc catgaaggct aatgttcctg tttacatttt tcctgccgac cagaaaaagg
     9361 gcgatattgt cacggattta cctggtggca agaaactgac gctcacgatg cgggatgttg
     9421 acctggaagc cgttgacaga cttcgcaaaa tgaagggaat tactgtgctg ggccatcctg
     9481 atgaaataag aaccatcgtc aaaaaacata ttgaaaataa aaaataattc agtgtttttc
     9541 gaattcaaaa taggtgcatg aattatcttt gcagcggatg atttttccgg ttacggtttt
     9601 attttttatt tcaagccaga tagcgcttgg ctctgcgatc cccggtactg ttgcactacc
     9661 cggggagagt aagagcactt cgcttttatg agctatgggg tgatggaagt gcccgaatat
     9721 caatacatca acgcccatct cttttgcaag atagcgaagg ccgtcggtat tttctgaggt
     9781 tagctgccct ttgtgtataa ccccaatttt tatttcttcg atatttatca ctttgcgctc
     9841 gggaaggaac ttcatgagtt caggactgtc ggtatcacca tgaaccgccg cgagttttcc
     9901 cagggcttca agttcattat aacaagtaag cgtttcaaaa tcccctgcat ggatgataag
     9961 gtctgaactc ttgattatcg aaataagttc ttccggaaga cgggaaagga gcgaatgccc
    10021 ctgttttatg tgggtgtccg aaaggattat cagattcata ctattactcc tgtggttttg
    10081 aaatagcaat tcaaatactc t
//
LOCUS       DATGBN010000439         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_578673, whole genome shotgun sequence.
ACCESSION   DATGBN010000439 DATGBN010000000
VERSION     DATGBN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578673"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VKL21_11065"
     CDS             complement(<1..1212)
                     /locus_tag="VKL21_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HMB46349.1"
                     /translation="MISIEINGQKLELNDNALLKDAIETAKAFYIPGTTIGILKTGDK
                     KEEATSEYKIITTKGEFRVELSGDSSLWTEFNHVFMDKRAHWETRNTIAFGPFETDIT
                     VERTEKQYNRYDVFLGTGGYDPKNSYLMIAKDKHISDYGSTKVSVIGKVISGKNIIAN
                     LKQGDAIMTIEPVIKWETLLDKITTFDLNTALQEGMKVFTYFNVDLINESPDGAEHFL
                     ALIRRKTFDVDTFSNSFISDNALQGEGCPYEHWDARSEGSVVVRTDGQGNGRIYIYKE
                     DRTSSPVHSVVGHVSHGIELIKIAPLNSNLAVISNPERVMVQGMTFMDAEKILNLRGL
                     KLEKRGYTGEDAIIVEQEPDTTIGIIEQGGVTALGVKSDKIINVKFYDDLAPITLDFF
                     RHSLRLKDRPLG"
     gene            complement(1242..>2308)
                     /gene="atwA"
                     /locus_tag="VKL21_11070"
     CDS             complement(1242..>2308)
                     /gene="atwA"
                     /locus_tag="VKL21_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HMB46350.1"
                     /translation="LVKNPMLLLADEPTGTLDPKTAELVHESIINATKKYNMSLIITS
                     HWPKVIEELSHRAIWLDDGKIIMMGKPADVATEFMKEVGSVGEQEEVKIGETIIRGRN
                     LQMTYFSMDRGIVRAVNDISFDINEGEIFGIIGVSGAGKTTTSKIVSGLLKPTSGSIE
                     VRIGDEWIDLTQLGPDKKGRATKYIGVLHQEYSLYPHRNVIDNLTESIGLDLPFELGE
                     RKAVHTLMTSGFTEKKAKEILVKMPDELSEGERHRVAMAQVLIKEPRILVFDEPTGTM
                     DPVTKIEVSRSILNARKELGETFVIVSHDMDFVAEVCDRAALMRLGKIVDIGETDAVL
                     SKLSSKEREEALEGIAIELK"
BASE COUNT          595 a          541 c          399 g          773 t
ORIGIN      
        1 acccaatggc ctgtccttaa ggcgcaggga atgcctgaag aaatcaaggg ttataggcgc
       61 caggtcatcg taaaacttta cgttaataat tttatcggat tttacgccaa gcgcggtgac
      121 tcctccttgt tcaataatcc ctattgtcgt atcaggttcc tgttctacaa taatggcatc
      181 ctcccccgta taacctctct tttccagttt taatcccctt aagttcaata ttttttccgc
      241 atccataaaa gtcatgccct ggaccatgac cctttcagga ttagaaatta cagcaagatt
      301 actattcaaa ggagctattt tgataagttc gatgccatgg gaaacatgcc caacaacgct
      361 atggactgga cttgatgtcc tgtcttcttt atagatgtat atacgaccgt tgccctgtcc
      421 atcggtcctg actaccactg atccctccga acgcgcatcc caatgttcat atggacatcc
      481 ttccccctgg agtgcattat cggaaataaa tgagtttgag aatgtatcca catcaaaggt
      541 ttttcttctt atcagggcaa ggaaatgctc ggctccatcc ggagattcat taataaggtc
      601 aacattaaaa taggtaaaaa ccttcattcc ttcttgaagc gcggtattca aatcgaatgt
      661 ggtaatcttg tcaagaagtg tttcccattt tatgacgggt tcaattgtca ttatagcgtc
      721 gccctgctta aggtttgcaa taatattctt accacttatt acctttccaa tgactgaaac
      781 tttcgtacta ccataatctg aaatatgttt atctttggcg atcataaggt aagaattttt
      841 tggatcatag ccgcctgttc cgagaaacac atcataccgg ttatattgtt tctcggttct
      901 ttccacagta atatctgttt caaacgggcc aaaagcaatt gtgtttcttg tttcccagtg
      961 agccctttta tccatgaaaa catggttgaa ttcagtccac aatgaggaat caccagatag
     1021 ttcaaccctg aattcacctt ttgttgtaat aattttatat tcagaggttg cttcttcctt
     1081 tttgtcgcct gttttcagga tacctattgt tgtgccgggt atatagaaag ctttcgctgt
     1141 ttctatagca tctttaagga gcgcattatc atttagttcc agtttttgtc cattaatctc
     1201 tattgatatc acatatcctc cgaatcttaa tgatattcga tctattttaa ttctatagct
     1261 ataccttcaa gagcttcttc ccgctcttta ctggaaagtt ttgataaaac ggcatccgtt
     1321 tcaccaatat ctacgatctt tccaaggcgc attaaagcag ccctgtcgca aacctctgcg
     1381 acaaaatcca tatcatgcga tactataacg aaagtctctc ccaattcttt cctggcattc
     1441 agaattgatc ttgaaacttc gatcttggtg acaggatcca ttgttcctgt cggttcatca
     1501 aaaacaagaa ttctgggctc tttgatgagg acctgtgcca ttgccactct atgcctttcc
     1561 ccttcactca attcatcagg catcttgaca agaatttctt ttgccttttt ttccgtaaaa
     1621 cctgatgtca tcaatgtatg gactgctttt ctttctccca gttcaaatgg tagatcaaga
     1681 cctatggact ctgtcaggtt atctataaca ttcctgtgtg gataaaggct gtattcctgg
     1741 tgcaatacac caatatattt tgtagcgcgt cccttcttat caggtccaag ctgtgtaagg
     1801 tcgatccact catccccgat ccttacttca atggatccgg aagtaggttt cagtaatccg
     1861 gaaactatct ttgatgttgt ggtttttcct gccccgctga ccccgattat tccgaaaatt
     1921 tcaccttcat taatatcaaa actgatatca tttacagccc tgactattcc cctgtccatg
     1981 gaaaaataag tcatttgcag gttccgtccc ctgataatag tctctccgat cttaacttct
     2041 tcctgctcgc ccactgatcc gacttctttc ataaattcag ttgcaacatc agcaggtttt
     2101 cccatcatta tgatttttcc atcatcaagc caaattgccc tgtgtgaaag ctcttcaatt
     2161 acctttggcc agtgggatgt gatgatcagt gacatattat atttctttgt tgcattaatt
     2221 atgctttcat gcacaagctc agcggtcttt ggatcaagag taccggttgg ctcatctgca
     2281 agcaatagca taggattttt aacaagct
//
LOCUS       DATGBN010000440         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_268602, whole genome shotgun sequence.
ACCESSION   DATGBN010000440 DATGBN010000000
VERSION     DATGBN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268602"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VKL21_11075"
     CDS             complement(<1..494)
                     /locus_tag="VKL21_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HMB46351.1"
                     /translation="MYNLLGATDNTGNAIDNWMDARQDDIRVIAQSRVTLASQKQYLK
                     EFLGTFEREHKGAYMEFYLLDLDGNITFSNLDRTGNESGKNYFIESSKGKIYVSDVYQ
                     SSTMGAPEIIIASPVSNNGDIVAVLAARVSLENLYRIIEKIDIGKSGEVFIVNQNGDI
                     IFHE"
     gene            complement(627..1142)
                     /locus_tag="VKL21_11080"
     CDS             complement(627..1142)
                     /locus_tag="VKL21_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB46352.1"
                     /translation="MNCDAIDNVIIELIKKNENISNFEISMILGIPEDEIAQRIGKIS
                     DTRSKILIVDDEIDTLLPLKRSLEVEDYIVVGASNGQEALIKARTDLPDLILLDLMMP
                     EMDGYEVCAKLKKDELTKNIPVIMLTAKDAVREKVKGLDIGADDYVTKPFHLNELKAR
                     IKSVLRRSKNQ"
     gene            complement(1139..1444)
                     /locus_tag="VKL21_11085"
     CDS             complement(1139..1444)
                     /locus_tag="VKL21_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMB46353.1"
                     /translation="MQNPNFLEKIFGKTAQLVVLEHLLENKDNDTYLSGIAAVTGLSH
                     STVSRVIGPLLEINIITEKPLGKQIRVFRLNKESELTKYLLKINDDLKHMITGGEKL"
     gene            1920..>2670
                     /gene="uvrC"
                     /locus_tag="VKL21_11090"
     CDS             1920..>2670
                     /gene="uvrC"
                     /locus_tag="VKL21_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMB46354.1"
                     /translation="MTKKIELIKKLPVSPGVYIMKDRENKVIYVGKAKSIRDRVSQYF
                     REQESPKNRLLMKNMENLEYIAAGNEIEALVIESNLIKEHRPRYNVKLRDDKNYPFIR
                     ITNEKFPRICISRRRGNDGAKYFGPYPGSKTVREVIKMASSLGIRKCKKKIPCPPCLN
                     YHIKQCAAPCLGNITKEEYLEIIDHVTSFLKGKHNQLIESLEKEMQRYSERQDYEKAA
                     RIRDQINALLELSEKQRVNLPGRKEQDIIAYA"
BASE COUNT          804 a          524 c          498 g          844 t
ORIGIN      
        1 ttctcatgga aaattatatc tccattctgg ttgacaataa atacctctcc tgattttccg
       61 atatcaattt tttctattat cctgtacagg ttctcaaggc tgactcgtgc tgcaaggact
      121 gctactatat ctccattatt gcttacagga ctggcaatta ttatttcagg ggcgcccatg
      181 gtactggatt gatatacatc cgatacatag atcttcccct ttgaagattc aataaaataa
      241 ttttttccac tttcatttcc ggtgcggtca agatttgaga aagttatgtt tccatcaagg
      301 tcgagcaaat aaaactccat gtaagctccc ttgtgttcac gttcaaaagt gcctaaaaat
      361 tcttttaaat attgtttctg cgatgccagt gtgacccttg attgggctat tacacgaata
      421 tcatcttgtc ttgcatccat ccagttgtcg atcgcatttc cggtattatc ggtcgcccca
      481 agcaagttat acatcaccga ttcgcggatt gcctccttac tgaaatcata agatatgtat
      541 acgactgcgg ataaaggtac tattgatatt accagaaaaa agataatcag ttctgacctt
      601 aacctcacat atccctctca tgctcattat tgattttttg accttcgaag aacacttttt
      661 atgcgggctt ttaattcatt aaggtggaaa ggtttagtaa cgtaatcatc ggcgccgata
      721 tcaagacctt ttactttttc cctgactgca tcttttgcag taagcataat gactggaatg
      781 tttttggtca gttcatcttt tttcaatttg gcgcacactt cataaccatc catttccggc
      841 atcataaggt caagaagtat tagatcggga agatcggttc ttgccttaat gagcgcctcc
      901 tggccattgc ttgcgcccac gacaatgtaa tcttcaactt caagagatct ctttaagggt
      961 agaagtgtat cgatctcatc atcaactatc agtatctttg atcttgtatc agaaatcttt
     1021 ccgatccttt gtgcaatttc atcttcagga attccaagta tcatggaaat ctcaaaatta
     1081 gaaatattct catttttctt tattaattca attatcacat tatcaatagc gtcacaattc
     1141 atagtttttc accaccggtg atcatatgtt tcaggtcatc attgatcttc aggaggtatt
     1201 ttgtcagttc actttccttg ttcaacctga acacacggat ctgttttcca agtggttttt
     1261 ccgtaattat atttatttca agaagcggcc ctattaccct tgatacagta gaatgggaaa
     1321 ggcctgtaac tgccgcgatt cctgacaggt atgtatcatt gtccttattt tcaagcaaat
     1381 gctcaaggac taccagttgt gctgttttgc caaagatttt ctctaaaaaa ttggggttct
     1441 gcataaatat gattaatatt accccattgt tatataacat tatctttttt tagcatacat
     1501 agatttcaaa gtttaaacca gggtatgaat ttaattttgt ccccatattt ataatcccct
     1561 ggattgtata gggaatggtt tgaccgaaaa tattaaatac tatttgttat atctaaagac
     1621 ttgtcgatta atagtcggga atcggtatgg gtggctatcg acaggttggg acgtggaggt
     1681 taataaaatc tcaagttcaa atgatcaata tgggaagagc ttcttcaatg ctgctggaag
     1741 aacctcatgg cctgggatta ttacctccct ctcaatctcc ctttttgcat aaattattgg
     1801 agccatattc ttacttcatt tatagatgct acatttttga atctaagggt tatattaata
     1861 tatatgacct attttatacg aattaacaat aagagggcag cattctctta aattttcgta
     1921 tgacaaaaaa aattgaactt ataaagaagc ttcctgtttc ccccggtgtt tacattatga
     1981 aagacaggga aaataaggta atttatgtgg gaaaagcaaa atcaatacgg gaccgggtaa
     2041 gccagtattt cagggagcag gaatcaccta aaaaccgcct cctcatgaaa aatatggaga
     2101 atctggaata tattgcagca ggaaatgaga tcgaggcatt ggtaattgaa tcaaatctga
     2161 tcaaagaaca caggccgcgc tataatgtaa agctcaggga tgacaaaaat tatcctttta
     2221 taaggataac caacgagaaa ttcccgcgca tttgcatttc ccgcaggcga gggaatgacg
     2281 gggcgaagta tttcgggcca tatccgggtt cgaagaccgt aagggaagtg ataaaaatgg
     2341 cttcaagcct tggcatcagg aaatgcaaaa agaaaattcc ctgtccgccc tgcctcaatt
     2401 atcatattaa acaatgtgct gcaccctgtc tcggtaatat cacgaaagag gaatatctgg
     2461 aaataataga tcatgttaca agtttcctta aaggaaaaca caaccagctt atagaatcac
     2521 ttgaaaaaga aatgcaacgg tattcagaaa gacaggatta tgagaaagct gcccgaattc
     2581 gcgaccagat caatgcctta cttgaacttt ccgagaagca gcgggtgaat ttgcccggga
     2641 ggaaagaaca ggatataatt gcatatgcag
//
LOCUS       DATGBN010000441         7779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_462147, whole genome shotgun sequence.
ACCESSION   DATGBN010000441 DATGBN010000000
VERSION     DATGBN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462147"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VKL21_11095"
     CDS             <1..849
                     /locus_tag="VKL21_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HMB46355.1"
                     /translation="KAYEALGVRAECSCTPYNIYDDLANFGDHVAWSESSAISYANSV
                     IGARTNREGGPSALSAALVGKTPNYGFHLDENRHPVIHIEVEPQLYDSDYGALGYIVG
                     EMIGDKVPIFDLKSIPTNDELKSLGAAMAASGSVALYHVRGITPEAGKYELPFEKITI
                     QEKLIKEVYKSGEPDLIAFGCPHSSIGELEKLARLLEGKKVKKEVWICTSRALKNRHP
                     GLIRRIEKSGAKVFCDTCMVVSPASEKFKCMMVNSGKAHKYAPNLCGVKSILASTEDC
                     IKAAYR"
     gene            883..1632
                     /locus_tag="VKL21_11100"
     CDS             883..1632
                     /locus_tag="VKL21_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMB46356.1"
                     /translation="MSSVTFLGTGGGRMVVLNQMRKSGGFWLKLDGANILHDPGPGSL
                     VMVHKLGLKPRDLDAIILSHRHIDHSNDVNVMTEAMTGGGFTPRGRLIVPSDCLFPDP
                     VILQYVRPFVEITEIKKGLEMTFKDIMIKFPLKNKHPVETYGVIYTIKSGSLGYIPDT
                     EYFPELVQAYAGVDYLIINVVRMKTDKRIRHLNMDEAAEIINKIRPKKAILTHFGLQV
                     LKSSPEYQAKIISERTGFEVIAAHDGMTVNF"
     gene            1930..3255
                     /gene="glnA"
                     /locus_tag="VKL21_11105"
     CDS             1930..3255
                     /gene="glnA"
                     /locus_tag="VKL21_11105"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HMB46357.1"
                     /translation="MAMTNEDILKSIEENQVRFLRLQFVDIGGMVKNVGIPVSQAEKA
                     LKSGIAFDGSSIEGFVRIEESDMILKPDNNTFMILPWDVSGGKVARLICDVYKPNNKP
                     FEGDPRYVLKRAIQAAAKKGFIMNNGPELEFFFFKRKDGQATCIPHDFGGYFDFPPVD
                     QAEDIRQNIVVALENMGFNIEASHHEVSIGQHEIDFKYADALKTADNVITFKFVTKTI
                     AQKYDLHASFMPKPIFGINGSGMHTNISLFKDGKNAFFDESKDMQVSDTLRYFVGGLK
                     KHVQSFTAVTNPIVNSYKRIVPGYEAPVYIAWSGANRSSMIRIPAARGMSTRVELRSP
                     DPSCNPYLSFAVILMAGLDGVENRIDPGEPTTLNLFHLNDEERKKHGIESLPGSLKEA
                     LDNLETDSVIKDALGEHVYQDFMRLGRAEWDAYRISVHDWEITRYLNII"
     gene            3306..4295
                     /locus_tag="VKL21_11110"
     CDS             3306..4295
                     /locus_tag="VKL21_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HMB46358.1"
                     /translation="MNTTTIDSMVQRKLNEILRILYENQEPIGARLIADKMNDRGYPI
                     GERGVRYHLRILDERGLTKRQGYDGRIITERGTKELGNALVGDRMGFIITRIERLIYD
                     TSFDLNTGQGSVIVNTSIIDKKDIDGTLDIMGHIINEGYSFSPFIKIMDEGTSASDIN
                     IPEGKIGIATMCSITIDGILLKNGIPVTPKYGGLLGVKNRKLTGFEEIIVYSGTSIDP
                     MRIFISKKMTRVLDAVDTGSGKLLANLREIPMSSVMEAQKVLKSAMSMGIVEIAKIGE
                     PGRSVLDAPVDSGKVGVVVYAGTNIMAAVEESGINVATYPISTIMDFKDLKKL"
     gene            4298..5272
                     /locus_tag="VKL21_11115"
     CDS             4298..5272
                     /locus_tag="VKL21_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMB46359.1"
                     /translation="MNAQIKAELISIGAIDIDPSILGRLTIPTAGPGAGGKAFFFKSG
                     GRRVRLVVDEGSPLRAVKENGEIVILKGGREFVRGTIEEELIHCPEQAYITMSERCIY
                     DCKFCPVPKLNGKVKSSEELLSLVEEAFKLGKMKGISITSGVEVSPEKEVERAAELVG
                     KLKKYNVPIGVSVYPTKDSSCILKEAGASEIKYNVETMDRELYEKYCKKPPLDFTLIC
                     LKDAVRIFGKNNVYTNFIAGLGETDETIEKNYEELAKMGVIPIIRAVGVHPLRFGELE
                     AERPTAQRLLKLAKMSRRILDQYGLDASVAKTMCLPCTGCDLNPHIDL"
     gene            complement(5335..6144)
                     /locus_tag="VKL21_11120"
     CDS             complement(5335..6144)
                     /locus_tag="VKL21_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMB46360.1"
                     /translation="MKKIRLQKSKAWKCSVCGYIHKGNEPPEECPQCGSSPKEYSELK
                     DKQKLGYDGKKSDVLLINGSSHRGHNTGVLVDLAEKALKRKGVSYRLFNLNELDIQHC
                     WCCYSMRDSACTYPCRNQLDDMPALQEMIVNSRAVIVASPINWNNMSARLKDFLDRLT
                     CLQNLYLLKKEGLTTGKVVGILVNGHEDGGMKTAMDIFIYFQQMGYVLAPFGFVYRTH
                     GAQYNAKTDDDFFKNDNILKKEVEGIVSNVIETMNQDLETKLRDKIVPVSE"
     gene            complement(6116..6340)
                     /locus_tag="VKL21_11125"
     CDS             complement(6116..6340)
                     /locus_tag="VKL21_11125"
                     /inference="COORDINATES: protein motif:HMM:NF022420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HMB46361.1"
                     /translation="MHDFKVPQCMCGICLTYIECREETAFCLSTTGKSKCIKEEKGCL
                     CPGCPIHEKMEFKYVYYCIRGNEKDQAAKK"
     gene            complement(6320..6481)
                     /locus_tag="VKL21_11130"
                     /pseudo
     CDS             complement(6320..6481)
                     /locus_tag="VKL21_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6573..6983)
                     /locus_tag="VKL21_11135"
     CDS             complement(6573..6983)
                     /locus_tag="VKL21_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46362.1"
                     /translation="MVFDIKTQAIANLTIQVLLIMTVSGAVFLVKKRNPGKHCVVMRV
                     AVLLQIIAIAGVMLPSMLGYIENEQRGIFFNIEMLVHHTLGLIVIAIWIYINLVFAGV
                     MRIRVRLDTVMRLALASWILALIIGLHMYFLIWS"
     gene            complement(7091..7387)
                     /locus_tag="VKL21_11140"
     CDS             complement(7091..7387)
                     /locus_tag="VKL21_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46363.1"
                     /translation="MRKDIKDLTSFKSDEIKWFFEFFKNTHINNKFNPGQKVNFLVTL
                     ILITGLSLSGFVIWMKSIFNVSFVELNFRAHDFLVELSIIMISGHEHYPKWRQK"
     gene            complement(7465..7725)
                     /locus_tag="VKL21_11145"
                     /pseudo
     CDS             complement(7465..7725)
                     /locus_tag="VKL21_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT         2368 a         1573 c         1701 g         2137 t
ORIGIN      
        1 aaagcttatg aggcgctggg tgtcagagcc gaatgctcct gcaccccgta taatatctat
       61 gatgatcttg caaactttgg ggaccatgtt gcctggagcg aatcttcggc gatttcttat
      121 gccaactcgg ttatcggggc acgaaccaac agggaaggag ggccaagcgc actttcggca
      181 gcactggtcg ggaaaacacc gaattacggc ttccacctgg atgagaacag gcatccggtc
      241 attcacatcg aagtagagcc acagctttat gattcggatt atggcgccct gggatatatt
      301 gtaggtgaga tgataggcga taaagtcccg atttttgatc tgaaatccat ccccacaaac
      361 gatgaactga aatccctcgg cgctgcaatg gcagcttcag gctctgtggc gctgtatcat
      421 gtccgtggga ttacaccgga agcgggtaaa tatgaactgc ctttcgaaaa aataaccatc
      481 caggaaaagc tgataaagga agtatataaa tccggtgaac ctgacctgat agctttcggg
      541 tgtcctcaca gcagtattgg cgaacttgaa aaactggcgc gacttcttga gggaaaaaag
      601 gtgaaaaagg aagtatggat ttgcacctcg cgcgccttaa agaacaggca tcccggattg
      661 atacggcgaa tcgagaaaag cggcgcaaag gttttttgcg atacctgcat ggtagtgtcg
      721 cctgcaagtg aaaagtttaa atgcatgatg gtaaactcag ggaaagcgca taagtatgcg
      781 cccaaccttt gtggcgttaa aagtatcctg gcaagtacgg aagattgtat taaggcagca
      841 tatagataat tttttgtata ggtcatacat ttataataat atatgtcaag tgtaactttt
      901 cttggtacgg gagggggcag gatggtcgtt ttgaaccaga tgcgaaagag cggaggtttc
      961 tggcttaaac ttgatggcgc gaatatattg catgatcccg gcccgggctc tcttgttatg
     1021 gtacacaagc tggggctcaa gccaagagac cttgatgcta ttattttatc ccacaggcat
     1081 atagaccatt ccaatgacgt gaacgtgatg actgaagcaa tgacgggagg tggtttcact
     1141 ccgaggggac gcctgatcgt gccttcggat tgcctttttc ctgatccggt catacttcaa
     1201 tatgtgaggc cctttgttga aataactgaa ataaaaaaag ggctggaaat gacttttaaa
     1261 gatattatga taaagttccc tctcaaaaat aaacatcctg ttgaaactta tggtgtcatt
     1321 tacacaatta aatccgggag cctggggtat attcccgaca cggaatattt tccggaactg
     1381 gtacaggctt atgcaggagt tgattacctg atcattaatg ttgtaaggat gaagactgat
     1441 aaaaggatca gacatcttaa catggatgaa gcagcagaaa taatcaataa gatcaggccg
     1501 aaaaaggcca tattgaccca ttttggctta caggtactga aatcatcacc agagtaccag
     1561 gcgaagatca tatctgaaag aacaggcttt gaagtcattg ctgcacatga tgggatgacc
     1621 gtgaattttt agcatataag atgattttcc ttgcgatgaa ccagttctcc agaaaataat
     1681 aaaaaatctc cagattccaa tagaaaacta tttaagtatg caaacggtag cctatagtcg
     1741 gtcattacat atccttcatt tgagttattt ctcaagttgg gtaaagtaag ggtattgatt
     1801 catcataaaa gaatcaataa aaccagatga ggatatgtaa ggaccataaa accaaaggag
     1861 aaaagaaaca caacacagaa tgatttataa tcagatatag taataaagtt agaaagataa
     1921 gtgatagata tggcaatgac aaatgaagat attttaaaat ccatagagga aaaccaggtc
     1981 aggttcttaa ggctccaatt tgttgacatc gggggcatgg taaagaacgt aggcatccct
     2041 gtatcacagg cagaaaaagc cttgaaatca ggaatagcat ttgacggctc ttcaatcgag
     2101 ggttttgtga gaatagaaga atccgacatg atactgaaac ccgataataa tactttcatg
     2161 atccttccct gggatgttag cggtggcaag gtcgcaagac tgatatgcga tgtttacaaa
     2221 ccaaataaca aaccattcga aggcgaccct cgctatgtct taaagagagc gatacaggca
     2281 gcggccaaga aaggtttcat tatgaacaac ggtcctgagc ttgagttctt tttcttcaag
     2341 agaaaagacg ggcaggcaac atgtattccc catgacttcg gcggctactt tgattttcct
     2401 cctgtggacc aggctgaaga tatccggcag aatatagtag tagcccttga gaatatggga
     2461 ttcaacatag aagcttctca tcatgaggtc tcgataggac aacatgaaat agatttcaaa
     2521 tatgcagatg cgctaaagac agctgacaat gtcattacat tcaagttcgt aaccaagacc
     2581 atcgcacaga aatacgacct gcatgcatct tttatgccaa aaccaatatt cggcataaac
     2641 ggttcaggta tgcatacgaa tatttctctc tttaaggatg gtaagaatgc attcttcgat
     2701 gaatcaaaag atatgcaggt tagcgatacg ctgcgatatt tcgttggcgg cctgaagaaa
     2761 catgtccaat cctttactgc agttacgaat cccatcgtga acagctataa gaggatcgta
     2821 ccgggttatg aagcgccagt atatatcgca tggtcaggag ccaaccgctc atcaatgata
     2881 cgcataccgg ctgcaagagg aatgagcacg cgcgttgaac tgcgaagccc tgacccatcc
     2941 tgtaatccat atctatcctt tgcagtgatc ctgatggctg gacttgacgg agttgagaac
     3001 cggatagatc cgggcgaacc cacaacactt aacctgttcc atctaaatga tgaggaacgc
     3061 aagaagcacg ggatcgaatc acttcccggc agtcttaaag aagcgcttga taacctggaa
     3121 accgacagcg taatcaaaga tgcgctcgga gaacatgtat atcaggattt catgagactt
     3181 ggaagggcag aatgggatgc atacaggata agcgtacatg actgggagat aacccgttac
     3241 ctgaatatta tataatcgga agttgtatta caagaaagtc gatcacccgc acctaaagga
     3301 gtattatgaa cacaacaaca atagactcaa tggtacaaag aaaactcaat gagatactga
     3361 ggatactcta tgaaaaccag gagcctattg gggcgcggct tattgccgac aagatgaacg
     3421 atcgcggata ccccatcgga gaaagaggtg tccgctatca cctccggatc cttgatgagc
     3481 gtggccttac caaacgccag ggttacgatg gcaggataat tacggaacga gggactaagg
     3541 aacttggaaa tgcactggtc ggggacagga tgggttttat cataacaaga attgaaagac
     3601 ttatttatga tacttcattt gacctgaata ctggacaggg aagtgtgatt gtcaatactt
     3661 cgataattga taagaaagac attgacggaa ccctggatat catggggcat ataattaatg
     3721 aaggctattc tttcagtcct tttataaaaa taatggatga aggtacatca gcttctgata
     3781 tcaatatccc ggaaggcaag attggaattg ccactatgtg tagcataacc atcgacggga
     3841 ttttgcttaa aaatggcatt cccgtcacgc caaagtatgg cgggttactg ggtgtgaaaa
     3901 acaggaagct aacagggttt gaggaaatta ttgtttatag cggtacatcc attgatccca
     3961 tgaggatctt tatctcaaag aaaatgacac gggttcttga tgcggtcgat acgggttcgg
     4021 gcaagcttct tgcaaacctg cgggagatcc caatgtcatc ggtcatggag gcgcaaaaag
     4081 ttttaaaatc tgcaatgagt atggggattg tggaaattgc caaaatcggg gaacccgggc
     4141 gctcagttct ggatgcgccg gttgattccg gaaaagtagg tgttgtagta tatgccggga
     4201 caaatatcat ggcggccgtt gaggaaagcg gaataaatgt tgctacatat ccgatatcca
     4261 ctatcatgga ttttaaggat ttgaaaaaac tatgaaaatg aatgcgcaaa taaaggctga
     4321 actcatttct atcggggcaa ttgatattga tccttcaatt cttggcagac tcacaattcc
     4381 cacggccgga ccgggcgctg gcggcaaggc ttttttcttc aaatcaggcg ggcgccgtgt
     4441 gaggctcgtt gtggatgagg gctcgccgct tcgcgccgta aaggagaatg gcgaaatagt
     4501 tatcttaaaa ggtggcaggg agtttgtaag gggtacaatt gaggaagaac ttatccactg
     4561 tcctgagcag gcgtatatta caatgagtga gcgctgcatt tatgactgca agttctgccc
     4621 cgtcccgaaa ttgaatggca aagttaaatc ttcagaagaa ttactgtcgc ttgtagaaga
     4681 ggctttcaaa ctggggaaga tgaaaggcat atcgataaca tcgggggtgg aagtatcgcc
     4741 cgaaaaagaa gtggaaaggg cggcagagct tgtcgggaaa ctgaagaaat acaatgtccc
     4801 gatcggtgtt tctgtctatc ccacaaaaga ctcctcgtgc atactgaaag aagcaggagc
     4861 aagtgaaatt aaatataatg tggagaccat ggacagggag ctttatgaaa aatattgtaa
     4921 gaaaccccct cttgatttta ccctgatttg cctgaaggat gctgtcagaa tattcgggaa
     4981 aaacaatgtt tatacgaatt tcattgcagg gctcggggag actgatgaaa ctattgaaaa
     5041 aaattatgag gagctggcaa aaatgggcgt tatcccgata atccgtgctg ttggcgtcca
     5101 tcccctgcga tttggtgaac tggaggccga acgcccaaca gcccaaaggc tgctcaaact
     5161 tgcgaaaatg tcgagaagga tacttgatca atacgggctt gatgcgagcg tggcaaagac
     5221 catgtgtctg ccatgcaccg gatgcgacct gaacccgcac atcgatctat gagtcataaa
     5281 aatttagaca ccggaaataa aaggttgcat attctcaggc gaacttcatt aacatcattc
     5341 actaacagga actattttat ctcttagttt agtctctaaa tcttgattca ttgtttcaat
     5401 aacattgctg acaatgccct cgacttcttt ttttaatatg ttgtcatttt tgaaaaaatc
     5461 atcgtctgtt tttgcattat attgtgcgcc atgggttcta tatacaaaac cgaatggagc
     5521 aagaacatac cccatttgct gaaaataaat aaaaatgtcc attgcggttt tcattcctcc
     5581 atcttcatga ccgttgacta aaattccaac aacttttccg gtagttaagc cttctttttt
     5641 cagtaagtaa agattttgaa ggcatgtcag cctgtccagg aaatccttta atcgcgcaga
     5701 catattattc caatttattg gtgaagccac tatcactgct ctggaattaa ctatcatctc
     5761 ctggagtgca ggcatatcat caagctggtt cctgcatggg tacgtgcatg cagaatctct
     5821 catgctgtag cagcaccagc agtgctgaat atccaattcg ttcaaattga acagcctata
     5881 actcacgcct tttcttttaa gagctttttc tgctaaatcc actaaaacgc cagtattgtg
     5941 acctctgtga gaacttccat taatcaataa aacatctgat tttttcccat catatccaag
     6001 tttttgctta tcctttagtt ccgaatattc tttaggagaa ctgccgcatt gggggcattc
     6061 ttccggaggt tcatttccct tatggatata gccgcatacg ctgcatttcc atgctttact
     6121 tttttgcagc ctgatctttt tcatttccgc ggatgcaata gtaaacatat ttaaactcca
     6181 ttttttcgtg aatcggacag ccggggcaca aacatccttt ttcctcttta atgcatttgc
     6241 tttttccagt tgttgaaaga caaaatgctg tttcttctct gcattcgata tatgtgagac
     6301 agatcccaca catacattgg ggaaccttaa agtcatgcat aaacctgaga atatcgttct
     6361 tgtaatctat gaaacgatcg aagagatttt tcggcttact ctgactcttt cttccaagtt
     6421 tttttgattg atattgagtt atcaaaggtg atggattttc ctccattcca tttctaatta
     6481 taagtgagtt ttgcctcaat taaatctttc gccatcgact tattaaagta ttttggtcac
     6541 cattgatgat agcagaaatt ctattctcaa gttcaagacc aaatcaggaa atacatgtgt
     6601 aggccaatga tgagtgccag tatccatgat gccaatgcta acctcatgac tgtatcaagc
     6661 ctgactctta tcctcataac tcccgcaaat accagattta tataaatcca tatcgcaatt
     6721 actatcagtc caagcgtatg gtgcaccaac atctcgatgt tgaagaatat accccgttgt
     6781 tcattttcaa tgtaaccgag catcgaggga agcattacgc cggctatcgc tattatctgt
     6841 aacagcacag cgaccctcat gacgacgcag tgttttcctg gatttctctt ctttacgaga
     6901 aatactgcgc cagataccgt cataatgagc aaaacctgga ttgtcaggtt tgcaattgcc
     6961 tgggtcttaa tatcaaaaac catatgtctt catccttcag ttatattgta tctgtacgaa
     7021 agaaaatcca gagtgttatt tagctttctt gacgaaaagg aaagtataaa agcatgccca
     7081 aacttgcgac ttatttttgc cgccatttag ggtaatgctc atgacctgaa atcattatta
     7141 tcgataattc aaccagaaaa tcatgtgccc tgaagttcaa ttcaacaaaa cttacattga
     7201 aaatcgattt catccagatc acaaaccctg atagagacag ccctgttatc aaaatcaaag
     7261 taaccagaaa attcactttc tgccctggat tgaacttatt attgatatga gtgtttttaa
     7321 aaaattcaaa aaaccattta atttcatcac ttttaaaaga agtcaaatct ttaatgtctt
     7381 tgcgcatgta caagctccct gaaatatagg atattgcagg cattaaaata aaaggaatag
     7441 atgcataaat gtgtatcatt ttaattaagg ggtcatcaag aagcgatttt ggagctctga
     7501 atcattggca atccttatcg cttctgcttc atttttaact ataattactg gtgctaccgg
     7561 ggcaaatgtt tcctcattta taactttcat ttcttttgtt acatcagtaa ggataacagg
     7621 ggaataaaaa tacccattcc caggaagtct tcctcctccg agtaacactt ttgcaccctt
     7681 tgacaacgaa tccttcacct gatcctcaat gatctgtatc tgttcttttc ttacaagctt
     7741 aaaaccccga tctttgcagc ctgttcaaca tcagcatct
//
LOCUS       DATGBN010000442         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_34644, whole genome shotgun sequence.
ACCESSION   DATGBN010000442 DATGBN010000000
VERSION     DATGBN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34644"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..699)
                     /locus_tag="VKL21_11150"
     CDS             complement(226..699)
                     /locus_tag="VKL21_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMB46364.1"
                     /translation="MAAIFRKEGKTYDEIGLTLKYPLTTIRDWLSLIHIEGISRKSDI
                     KQTGKPKRLTEDQIKKLKPILLKSPEEQDFKFTIWTTKLVMELIKKLYDVSYKPLQVR
                     RILHRLGLSCQKPRPTHKKASKKAQEDFKKTSEKESDHMQKMDMRSYFWTKASSR"
     gene            complement(904..1074)
                     /locus_tag="VKL21_11155"
     CDS             complement(904..1074)
                     /locus_tag="VKL21_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46365.1"
                     /translation="MKSRHLEKTICKAISRVGDDSIANGSAHLASQNRSPYSGHRAIG
                     TPEEASISFDLY"
     gene            complement(1055..2071)
                     /locus_tag="VKL21_11160"
     CDS             complement(1055..2071)
                     /locus_tag="VKL21_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46366.1"
                     /translation="MDSEMPRRWLLISREAGLSSEEDSEGRWSVDHLFLDQDAIPTIV
                     EVKRSTDTRIRREVIGQILEYAANAVVYWPIESIRAKFDAQYKDKEKDSQEILSQFLG
                     DNSDLEQFWQKTKTNLQAGRVRMVIVADEIPSELQRIIEFLNGQMDPAEVLAVEIKQY
                     VGRGLQTLVPRVLGQTVEAIIKKTGGTRERKKWDEALFFKDLEKRSSTEEANVARKIY
                     DWSKARQLRGWWGEGKHNGSFIPVIDHNGTGYTLIALWTYGSVEIQFQYMTTKYMKTK
                     PFDDEAKRLELLHKLNEIPGISISEKFINLRPNFPLNNFKDEVNLKQFLETLDWAINE
                     IKTS"
     gene            complement(2038..2199)
                     /locus_tag="VKL21_11165"
     CDS             complement(2038..2199)
                     /locus_tag="VKL21_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46367.1"
                     /translation="MNGVIYLNQNNEQLVEMTEQKYDSEELLQTLIAKYPNLLAGIRW
                     IAKCHEDGC"
     gene            complement(2361..3545)
                     /locus_tag="VKL21_11170"
     CDS             complement(2361..3545)
                     /locus_tag="VKL21_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HMB46368.1"
                     /translation="MTAAILKGMMDLGHPQMGSEVAYAVPRKASQDDARIIETEHYKK
                     APFIGREFLMVGDCINDINEHWCTSPQSRELEYKEIKPARDKLDFEFKAFEVDHSMYG
                     SAAYAIDTSDGWIVYTGDLRLHGKFKDKTEKFVREAKSLDPKVLIIEGTRIGRQDRKE
                     SEDEVHSTCLGATAAEKGLVVADFSPRNFERLDTFMNIAKETGRQLVVLAKDAYMLDI
                     MKCADGMDRMSELLVYRDVKSKRDAFEKMVHEKFKGQLRDPVDIAKAPQQYILCFSFW
                     DVKHLLDIKPVGGTYIYSSSEAYTEEQVIDFGRLWNWMKFFDFKVRGFTIGENDGKLA
                     FEKGFHASGHASAAELLKIIEDIDPEIVLPVHTENPEFFVDNLKGYNVVLAQEGKGIE
                     IK"
BASE COUNT          989 a          837 c          630 g         1212 t
ORIGIN      
        1 atatcatgtc tcaaaaatca ataacaataa tcactttttt gagttgatca atcagtcgaa
       61 ttaagaaatt cttgaaaata tcactgttta atttatcaga aagttcataa ataaaatgtt
      121 ctgaactcaa agcccccata acacagtgtt tatcacttct cttatgctca tatttgatga
      181 caggttgact tcctttcaag aaccacccat atgtaagata tggttttatc gggaagatgc
      241 tttcgtccag aaataggatc tcatatccat tttctgcata tggtcggact ctttctcgga
      301 agttttttta aaatcttctt gagctttctt actggctttt ttatgagttg gtcgtggctt
      361 ttggcaggat aaaccaagac ggtgaagaat acgtcttact tgaagtggtt tataagaaac
      421 atcatatagt tttttgataa gctccatcac aagttttgtt gtccaaatcg tgaacttaaa
      481 gtcctgttct tcaggagatt tgagcagtat gggttttaat ttttttatct ggtcttctgt
      541 taatctcttt ggtttgcctg tttgctttat atcgcttttt cttgatattc cttctatgtg
      601 gataagactc aaccaatcgc gaattgttgt caaaggatat ttcaaagtca ggccaatttc
      661 gtcataagtt tttccttctt tgcgaaaaat cgctgccaat aaccgtattt ttgctttagc
      721 atttctttct ctatgataca aatatgtcaa tttttttgct gtataatttg gtaggaatgc
      781 ttctcctttt ggaactattg tcagttaaac cacctcagtc gacataggta ataattggtt
      841 gaagtagtat ttatatatta ctaaaattta ggaactatac tataattgac acttgacaag
      901 acactagtat aaatcaaacg aaatcgatgc ctcttctggc gttccaatcg cccggtgacc
      961 cgaatatggt gagcgatttt gggaagcaag atgggcgctg ccattcgcaa tgctgtcatc
     1021 gcccacacgg gagatcgcct tgcatattgt tttttcaaga tgtcttgatt tcattgattg
     1081 cccagtcgag tgtttcgaga aactgtttaa gattaacttc atccttaaaa ttatttagag
     1141 gaaaatttgg acgcagattg ataaactttt cggaaatgga aatccctgga atctcattta
     1201 gtttgtgtaa aagctctaaa cgcttagcct catcatcaaa tggttttgtc ttcatatact
     1261 ttgttgtcat gtattgaaat tgtatctcaa cgcttccata agtccaaagt gctatcaatg
     1321 tataacctgt tccattgtgg tcaattacag gaataaatga cccattatgt ttaccttcac
     1381 cccaccatcc tcttaattgc ctggcctttg accaatcgta aatctttctg gcaacatttg
     1441 cctcttcggt gctagatctt ttttctaaat ctttgaagaa caaagcttca tcccacttct
     1501 ttctttcacg cgtgccacct gttttcttta taatagcttc gacagtttga cccaatactc
     1561 taggaacaag tgtttgaaga cctctgccaa catattgctt tatttcaact gccaatacct
     1621 cagcaggatc catttgtcca ttcaagaact cgataatacg ttgaagttca gaaggaattt
     1681 catcagccac tattaccatg cgcactcttc cagcctgcaa atttgttttc gttttttgcc
     1741 aaaactgctc caagtcagaa ttatcaccaa ggaattggct aagaatttct tgtgaatctt
     1801 tttctttatc tttatattgt gcatcgaatt tagctcgaat tgattctatt ggccaatata
     1861 ctacagcatt ggctgcatac tcaagaatct ggccaattac ttcacgtctg atacgagtat
     1921 cagtgcttcg tttgacttct actatagtgg gaattgcatc ttgatcaaga aataaatgat
     1981 caacagacca tcgtccttcg ctatcttctt ctgaagacaa accagcctca cgagaaatca
     2041 acaaccatct tcgtggcatt tcgctatcca tctgatccca gccagaaggt ttggatattt
     2101 tgctatcagt gtctgcagca attcctcgga atcatatttc tgttcagtca tctcaacaag
     2161 ttgttcatta ttttgattta gatatattac tccgttcata gaacatataa ttgaacctca
     2221 gcctacatgg agtttttgat cttcatcatt atagtccgca gctctgctgc agttgggaaa
     2281 agacgtgatc aatgaaacct cagcgcctgt tcacatacgt tatccatgtc cataaaatgc
     2341 caggcatcat caaaatcacc tcatttgatc tcaatcccct tcccctcctg cgccagcact
     2401 acattatatc ctttcagatt atcgacaaaa aattcaggat tttcagtatg taccggcagt
     2461 acgatctcag gatcaatatc ttcaatgatc tttaacaact ccgcagccga cgcatgccct
     2521 gaagcatgga atcccttttc gaatgccagc ttaccgtcat tctcaccgat cgtgaaacct
     2581 ctaaccttaa aatcaaagaa tttcatccag ttccacaatc gcccaaaatc aatgacctgc
     2641 tcttcagtgt aggcttcact gcttgaataa atatacgttc cgcccacagg ttttatgtca
     2701 agcagatgct tcacatccca gaagctgaag cacagaatat actgttgcgg cgcttttgcg
     2761 atatccaccg gatcgcgaag ctgccctttg aacttttcat gcaccatctt ctcaaaagca
     2821 tcgcgttttg atttcacatc cctgtaaaca agaagctcac tcatcctgtc catgccgtca
     2881 gcgcatttca ttatatcaag catgtaggcg tcctttgcaa gaaccacgag ctgtctgccg
     2941 gtctcttttg cgatattcat gaaagtatca agccgttcaa agttgcgcgg tgagaaatcc
     3001 gcaacaacaa gacccttttc agcagcagtt gcaccaaggc aggtggagtg aacttcatct
     3061 tcagattctt tcctgtcctg tctgccgatg cgcgtacctt ctatgattag caccttcgga
     3121 tcaaggcttt ttgcttctct tacgaatttc tcggtcttat ccttgaactt cccgtgaagt
     3181 ctcagatccc cggtatatac gatccagcca tccgatgtgt caatggcgta agctgcggag
     3241 ccgtacatgg aatgatcaac ttcgaaagct ttgaactcaa agtccagttt atccctggca
     3301 ggtttgatct ctttgtattc aagttccctg ctctgcggag aggtgcacca gtgctcatta
     3361 atatcgttaa tgcagtcacc aaccattaaa aattctctgc ctataaaagg cgcctttttg
     3421 tagtgttctg tctcaattat ccttgcatca tcttgcgaag ctttccttgg aacggcatat
     3481 gcgacctcag accccatctg tggatgtcca agatccatca ttcctttaag gatagcagct
     3541 gtcatctggg atgacacgat cggtatattt acataattcc tcatagaaat cccctatttt
     3601 cttataattc gttccaaaat caaagaaaac tcctctgcca tcgaactcca ggtggatctt
     3661 atttccac
//
LOCUS       DATGBN010000443         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_423546, whole genome shotgun sequence.
ACCESSION   DATGBN010000443 DATGBN010000000
VERSION     DATGBN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423546"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2367
                     /locus_tag="VKL21_11175"
     CDS             <1..>2367
                     /locus_tag="VKL21_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46369.1"
                     /translation="NTSFAAAGISPATMVMMDDFIAAGTQIKVERPGKAAGIAPVDTI
                     DGPTVRLFNGDVLSIKTAEEAQKIRPSVKQILDIGEILINFGDFLENNHPLVPSSYCF
                     EWWLQELMAKTSIKEPGDLKNPDQEKALSLSDKFKVPLHPKYTYLWHDISIEEFSQLS
                     EYIHKNGTYSDRLSFALDDKIKNILEVLLVPHIVREKKVVIEEPLPLMRCLGLDTTLK
                     KTSMIMGANILDTVSKTSGITIRARAPIRIGARMGRPEKSDKREMKPAPHVLFPIGGA
                     GGKRRSLVDVKDFVDDEDISKEKNNEFKNSEFQTGLTYQKGKVGTINVQIGQRICPAC
                     QNRTFKNRCTCGKFTIPLLKCQSCGIEVSKPICPKCKKETTSVTEMNIDFISEYQKAL
                     DNLGERDVFKPKGVLGLTSKNKTPEPLEKGVLRAKHDVVMFKDGTVRYDLSDMPLTHI
                     RPVELGVSVLKFKELGYVNDTYGNPLLDENQILELKVQDIVVSKDCAEYLLKTAHFID
                     DLLTRYYKVESYYNIEKIDDLIGKLVIGLVPHTSAGVLARLVGFTTASVGYAHPFFHA
                     AKRRNCFQGDEKLLVQRGDNFELLTIRELVELNLIDDIEKDDFGTQYKKVKGLKTFAF
                     NIKTKKFQLADITHVSKHVSPEKLIEIKTKSGRKIVVTKDHMFPNRFGEKVRAEDADE
                     LLIPWNIRRPPVESKKNIDLLSITDPAEIMIRTECDVFDEDLSLSQISSNLGMSYKTF
                     TNYIYRQSYPLEIVNRFNPELIETGNYLLGSKRDRVSIKPSITMNEDF"
BASE COUNT          767 a          454 c          539 g          607 t
ORIGIN      
        1 ggaacacaag ttttgcagca gctggaatca gtcccgccac aatggtaatg atggatgatt
       61 tcattgcggc aggcacccag ataaaagtgg agcgtccggg aaaagcagcg ggtatagctc
      121 cggtcgatac catcgatggt cccacagttc gccttttcaa tggggacgtg ctcagtatta
      181 agacggcaga ggaagcccaa aaaatacgcc cgtctgtgaa gcagatcctg gatatcgggg
      241 agatactgat aaatttcggg gatttcctgg agaataacca ccctctcgtg ccctcatcct
      301 attgctttga atggtggctt caggaactta tggcaaaaac atcaataaag gaacctggcg
      361 acctgaaaaa tcctgaccag gaaaaagcgc tttctttatc agataaattt aaagtgccgc
      421 tgcatccaaa atatacatac ctgtggcatg atatttcaat tgaggagttt tcccagctgt
      481 ccgaatacat ccataaaaat ggaacatatt ctgacaggct ctcatttgcg ctggatgata
      541 aaataaagaa catccttgaa gttcttcttg tcccccatat tgtacgcgaa aagaaggttg
      601 tgatcgagga accgcttcct cttatgagat gtcttggcct tgatacgact cttaaaaaaa
      661 cttccatgat tatgggggca aacattcttg atacagtttc aaaaacaagc gggataacca
      721 tcagggcccg tgctcccatc aggatcggcg cgaggatggg acgtcctgag aaatccgata
      781 agcgtgaaat gaaacccgcg cctcatgtgc ttttccctat cggtggagcc ggaggaaaga
      841 ggcgatccct ggtagatgtg aaagatttcg tggatgatga ggatattagc aaagagaaaa
      901 ataatgaatt caagaacagt gagttccaga ctggacttac ataccagaaa ggaaaagtgg
      961 ggacaataaa cgttcaaatt ggccagcgca tatgtccggc atgccagaac aggactttca
     1021 agaacaggtg tacatgcgga aaattcacaa ttccgcttct taaatgccag tcatgcggca
     1081 tagaagtcag taaaccaata tgccctaaat gcaaaaaaga gactacatct gtcactgaaa
     1141 tgaatattga tttcatatcg gaataccaga aggcgcttga taaccttggg gaaagggatg
     1201 ttttcaaacc caaaggcgtt cttggcctta cttcaaaaaa caagacccct gaacctttag
     1261 aaaaaggggt gctaagggca aaacatgatg ttgtgatgtt caaggacggg acggtgagat
     1321 atgatctctc ggatatgcct cttacacata tcagacccgt ggaacttggt gtttcggttt
     1381 tgaaatttaa agaacttggt tatgtgaatg acacatacgg taatccgctt cttgatgaga
     1441 accagatcct tgaacttaaa gtgcaggaca ttgttgtttc aaaagattgc gccgaatatc
     1501 ttcttaaaac tgcacatttc atagatgacc ttttgacgag atattataaa gtcgaatctt
     1561 attataatat cgaaaagatc gatgatctga tagggaaatt ggtcataggg ttagtccctc
     1621 atacatcagc gggagtgctt gcccgccttg tgggctttac cacggcgtct gttggctatg
     1681 cccatccttt cttccatgca gcaaagcggc gaaattgctt ccagggggat gaaaaattat
     1741 tagttcaaag gggagataac tttgaactgt tgacgattcg agagctggtt gaactcaatc
     1801 ttatcgacga catagaaaaa gatgattttg gaacgcaata caagaaggtt aagggcctga
     1861 aaacatttgc tttcaacata aaaacaaaaa aattccaatt ggcggatata acccatgttt
     1921 caaaacatgt ttctcctgaa aagttgatcg aaataaaaac gaaaagcggc aggaagatag
     1981 tcgtaactaa agatcatatg tttccgaata gattcggaga aaaagtgagg gcggaagatg
     2041 cagatgaatt attgattccc tggaatatca gaagacctcc tgtcgaatct aaaaagaata
     2101 tagacctgct ttcgataact gatcctgcag aaattatgat aaggacagaa tgcgatgttt
     2161 tcgatgagga tttatctcta tcacaaataa gcagcaatct tggaatgagt tataagacat
     2221 ttactaatta tatttacaga cagtcatatc cgttagagat agtcaataga tttaatcctg
     2281 aattgattga gacaggaaat tacttgttag ggagtaaaag agacagggtt tcaataaagc
     2341 ccagcataac gatgaacgaa gatttct
//
LOCUS       DATGBN010000444         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_500868, whole genome shotgun sequence.
ACCESSION   DATGBN010000444 DATGBN010000000
VERSION     DATGBN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500868"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..482)
                     /locus_tag="VKL21_11180"
     CDS             complement(183..482)
                     /locus_tag="VKL21_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HMB46370.1"
                     /translation="MTCIVLSAFDYVFIFFFEIVIAFLILATKTAMDRLYHKLKPFYA
                     KLINAVHDELVFEVQEDRVTEAAQVIKDEMEAAGSEYLKSSPCIVEVVMGESWQK"
     gene            519..839
                     /locus_tag="VKL21_11185"
     CDS             519..839
                     /locus_tag="VKL21_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46371.1"
                     /translation="MDTKGTPLFHKHLTKPEIINICKHLVEKNGIRSIERITGHHRDT
                     IGDLIEDLALHAEFVNSILLQDIKLGQFEVDEMWTFIKKNKRTLSQEALIQISKVMPG
                     YSLS"
     gene            950..1366
                     /locus_tag="VKL21_11190"
     CDS             950..1366
                     /locus_tag="VKL21_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46372.1"
                     /translation="MKIQIFSDGNDDYTSTIPEYYVETCVDYGQLIKIREGGKVVDKI
                     KKVIYGTPNIDDIETTDIENMNSICRERLGRFVRKTKCFSKKNTRLINAFELFHFYWN
                     FMDKLTKTETPAMLEGLADHQWNWEQFFDFSLSILN"
     gene            1814..>2024
                     /locus_tag="VKL21_11195"
     CDS             1814..>2024
                     /locus_tag="VKL21_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMB46373.1"
                     /translation="MANSSNDIIEVIKTRRCVREYLDKEISDTDIKFLIDCAVYAPSG
                     LNMQPWGFLVIQNRDKIMQLSETCKK"
BASE COUNT          665 a          387 c          377 g          595 t
ORIGIN      
        1 tggagactgg attctggaga ctcacactaa gcagagatgg gtactgggaa gagctaagtg
       61 tgagtatctt ttcttcttct tccttgttta ctatactact aataccccta aaagctatca
      121 aatatcatat atctctatca gtttaaccac atcataaaaa ttacaagaat taaaagaaag
      181 gttcatttct gccacgattc ccccattact acttcaacaa tgcaaggact gcttttaaga
      241 tattcgctac ctgctgcttc catttcatct ttaattacct gggcagcttc tgtaacccgg
      301 tcttcttgaa cttcaaacac aagctcatca tgaaccgcat tgatcaactt tgcataaaat
      361 ggtttcaact tgtgatataa tctgtccatt gccgttttgg ttgccagaat gagaaatgca
      421 attactatct caaagaagaa gataaagaca taatcaaacg cggaaagtac aatacaggtc
      481 atcaaagata ttattgtaat cactgcaaga catttttcat ggatactaag ggaaccccct
      541 tatttcacaa acatcttacg aaacctgaaa taatcaacat atgtaaacat cttgtagaga
      601 aaaacggtat tcgatcaatc gaacgaataa caggtcatca cagggatacc attggagatt
      661 taatagaaga tcttgcctta catgctgagt ttgtaaattc cattcttctt caggatatta
      721 agcttggaca atttgaagtt gacgagatgt ggacattcat aaaaaaaaac aaaagaacgt
      781 tgagccagga agccctaatt cagatatcga aggtgatgcc tggttattca ctttcataac
      841 gagatgatcg tatcttattg tagcctatga gattggaaaa cgaacaagag aatcctgcaa
      901 aagattattt gaaaaagttt ttgatagagt acaattacca tttccaaatg tgaaaattca
      961 gatattttct gatgggaatg atgattacac ttccactatc ccagaatact atgtagagac
     1021 ttgcgttgac tatggacaat taataaagat tagggaagga ggtaaagtcg ttgataaaat
     1081 taagaaggtc atatatggaa ctcccaatat tgatgatatc gagacaactg atatcgaaaa
     1141 tatgaatagc atttgccgtg aaagattggg cagatttgta aggaaaacaa aatgcttttc
     1201 aaaaaaaaat acaagactca taaatgcttt cgaattgttt catttttatt ggaatttcat
     1261 ggataagttg acaaaaactg agactcctgc aatgctggaa ggtttagcag atcatcaatg
     1321 gaattgggag caattttttg atttttcatt aagtattcta aattaggaca ttgccaaata
     1381 tccagagtgc actttctcct tttttttgct ggtgtgaatc cacgggatct atggagactg
     1441 agctctatgg aaattcacat tgagcaaaaa tgagtactga gaaatgcata gtgtgagttc
     1501 ccaagcatat tttccagcca cctgcccgcg tatcccccaa acccccctgc ccgtctttgt
     1561 tcgtaaatat acgaagcata tatcagtcta tattatgggc gggcgggaat cagtacatcc
     1621 cacgccgcca ccaaatcatc aaaatatggg cgcgcgggac tccagtatac tttccgctga
     1681 ccaggtcttt ctacccgggc gcccccattt ccgctcatct tcgcggctga atctcaatca
     1741 gaaaaattct tgaccagaaa ggttatctaa ttttgaaatc tgttaatctg ttaataacaa
     1801 gggaaataat atcatggcaa attcttcaaa cgatattatc gaagtaataa aaacacgtcg
     1861 ctgcgttcgt gaatatctgg ataaagagat ttccgatact gatattaaat ttctcatcga
     1921 ttgcgctgtg tatgcccctt ctggcctgaa tatgcaaccg tggggttttc ttgtgataca
     1981 gaacagggat aagatcatgc agctttcgga aacatgcaag aagt
//
LOCUS       DATGBN010000445         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_269231, whole genome shotgun sequence.
ACCESSION   DATGBN010000445 DATGBN010000000
VERSION     DATGBN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269231"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VKL21_11200"
     CDS             complement(<1..269)
                     /locus_tag="VKL21_11200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMB46374.1"
                     /translation="MSKHIKAWEDEYGKSIWKGHYSLELLDPAVKKGRLLDAGCGSGK
                     YSVPLRMRGFDVVGIDVSFNALHMLKDSSKAREIDIDILAANVFQL"
     gene            318..1631
                     /gene="thiD"
                     /locus_tag="VKL21_11205"
     CDS             318..1631
                     /gene="thiD"
                     /locus_tag="VKL21_11205"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HMB46375.1"
                     /translation="MMKTALTIAGVDPGGGAGIAADIKTFAALGVHGTCIVTSVTAQN
                     TQGVLNSFDMPVCFIEEQFDAVVSDIRIDCVKTGMLSSPEIVRAVSGLIKREKLPLVI
                     DPVMTAEAGGILLKNEAVEVLIEELLPLSVIITPNVSEAQRLSGMNIRDIMDARKAAR
                     KISELGAKAVIVTGGHLNGTDILYSGGEFTQIEGKLIKGGTHGSGCTHSAVITAELSK
                     GATLIEAAHRAKEFVEQAITRSVNIGKGASPVNQIGHTLDDAHRYRVIRNIEEAFDLI
                     KKCKEFSNLIPEVGCNIAMGIPDASSITDIAAVSGRIVRLKNEPHAVGCVAFGASSHI
                     ARIVLTAMHYNDQMRAAMNIRYSDEALAACEDIGFCIASFRREDEPEGVSTMEWGISD
                     AVMREGKVPDVIYDKGGIGKEAMIRLLGRKAIEVAGYATKIALSL"
     gene            1694..2668
                     /locus_tag="VKL21_11210"
     CDS             1694..2668
                     /locus_tag="VKL21_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOG1 family protein"
                     /protein_id="HMB46376.1"
                     /translation="MIFEKIPTVPTAEELLDKSYRRATRAKRGKQILDRKSKLQAEES
                     MLLTAANILSDNLAHIVRKFPSLDQLPPFYLELAEVLVGVEEMKMNLASVQWAGEKIG
                     TLARKYVGMMREADDAKIVRKQAFGRISSIVGSIDGNLRFLNEARNKLRKLPAIDEGP
                     TIVVAGYPNVGKSSFVALVSSAHPEIASYPFTTKGLAVGHFTRNKVRHQVIDTPGLLD
                     RPLEERNEIELQAITALNHVGKVILYIIDPSETCGYSLDTQLHLLDEIKNRFTAPFLI
                     VANKKDISETEIGDMSMSTLKGEGVDAVLERLLEMMPITDTLVSDEIA"
     gene            2895..4025
                     /locus_tag="VKL21_11215"
     CDS             2895..4025
                     /locus_tag="VKL21_11215"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMB46377.1"
                     /translation="MVALILLVAFASVTTAHGLSEKEKLGKDLFFDKDLSDPAGQACA
                     ACHAPEVGWTGPDKNINAHGAVYEGAVKGRFGNRKPPSAAYAGDSPILTNTTPFTGGM
                     FWDGRATGWTLGDPLAEQAQGPFLNPLEQNNPNASAVVNKVKASSYVRLFYNICGKGK
                     TDADYYVCIARSIAAYERSSEVSSFTSKYDYYLKGGAILTMTEKRGLKLFTGKGNCSN
                     CHVSTGSKPLFTDFTYDNLGVPKNLENPFYNEPAYNPMGSAWIDEGLGGFLKGIGYPS
                     YGEVGKMKVPTLRNVDKRPYNGFVKAYGHNGYFKSLEEIVHFYNTRDVPGAGWKGKPW
                     PAAEVSANVNNIELGNLGLTHQEESDIVAFLKTLSDGYTPTK"
     gene            4269..>4539
                     /locus_tag="VKL21_11220"
     CDS             4269..>4539
                     /locus_tag="VKL21_11220"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMB46378.1"
                     /translation="MLSKRIWICAIILLLTAATAAALNDTEQLGKNIFFHKELSIDKN
                     QACAACHSPQVGFTGGDSIINVMGSVYKGSIAGRFGNRKPPAAAYA"
BASE COUNT         1392 a          870 c         1097 g         1180 t
ORIGIN      
        1 agttgaaaaa catttgcggc aagaatatca atatcaattt cacgggcttt gctgctatct
       61 tttagcatgt gcagggcgtt gaatgacaca tcaattccca ccacatcaaa tccccgcata
      121 cgcaaaggca ccgaatattt accgcttccg cagccggcgt caagaagtcg tccttttttt
      181 acagcgggat caagcagttc aagtgaataa tgacccttcc aaatactttt tccatattca
      241 tcttcccatg ctttgatatg ttttgacata atcttatttc gactggaacc attataatgt
      301 atggagatta tttttgcatg atgaaaacag cattgacaat agcgggcgtt gatccgggcg
      361 gaggggcggg aatagccgcg gatataaaga catttgccgc cctcggggtt cacgggacct
      421 gtatagtaac atcggttacg gctcaaaaca cacagggggt ccttaattca tttgacatgc
      481 cggtttgttt catcgaagag caattcgatg ctgttgtcag cgatataaga atcgattgcg
      541 tcaaaacagg catgctttca tcgcctgaaa ttgtaagagc agtatctgga cttataaaaa
      601 gggaaaaact gcctcttgtt attgatcctg taatgactgc cgaagcagga ggaatcctgc
      661 tgaaaaatga agcggtagag gttttaattg aggaactact gcctctttca gtaattatta
      721 ctcctaacgt gtcagaagcg cagaggcttt ccgggatgaa tatcagggac atcatggatg
      781 ccaggaaggc agcaaggaaa atttcagaac tgggagcaaa agccgtcata gtcacaggag
      841 gccatcttaa tggaaccgat attctttatt caggagggga attcacacag atcgagggca
      901 aattgataaa gggaggcact cacgggtcgg gatgcactca ctctgcggtt attacagccg
      961 aattatcaaa aggagcgact cttatagaag cggcgcatcg tgcaaaggaa ttcgttgaac
     1021 aggcaataac aagatcagtt aatataggaa aaggggcatc accggtaaac cagatagggc
     1081 atacccttga cgatgcccat aggtatcgcg ttatcaggaa tatagaggaa gcatttgatt
     1141 tgatcaaaaa gtgtaaggag ttctcaaacc ttattcctga agtcggatgt aatatcgcta
     1201 tgggtatccc ggatgcttca tcgataacgg atattgccgc agtttcaggc aggatagtaa
     1261 ggttgaaaaa tgaacctcat gcagtaggtt gtgtggcatt tggcgccagc agccacattg
     1321 cccggatcgt attgaccgct atgcattaca atgatcagat gagggctgcg atgaatatcc
     1381 gctattcgga tgaggcgctt gccgcatgcg aggatattgg gttttgcata gcctcattca
     1441 ggagagagga cgagccggaa ggagtatcca ctatggaatg gggcatatcg gatgcagtaa
     1501 tgagagaagg aaaggttccg gatgttattt atgacaaagg cggtatcggg aaggaagcga
     1561 tgataaggct tctggggcgg aaggctattg aagttgcggg atatgcaaca aagatagcat
     1621 tatctttatg aactcaatga gcatattagt tccttcagca cttatttaaa ttaaattata
     1681 attccggaca aatatgatat tcgaaaaaat acccactgtt cccacggcag aagaacttct
     1741 tgataaatcc taccgccgcg ccacccgggc taaacgcgga aaacaaatac ttgacaggaa
     1801 atccaagcta caggctgaag aatcaatgct gttgacagcg gcaaatatcc tcagcgacaa
     1861 tcttgcccat atagtcagaa aattcccgag tttggaccag ctgccgcctt tttatcttga
     1921 gcttgcagag gtactggtgg gtgtcgaaga gatgaaaatg aacctggcct cggtacaatg
     1981 ggcaggggag aaaataggca ccctggcaag aaaatatgtg ggtatgatgc gcgaagcgga
     2041 tgatgcaaag atcgtccgga aacaggcatt tggcaggatc tcatcgatcg ttggcagcat
     2101 cgatggaaac ctgcgcttcc ttaatgaagc gcgtaataag ctaagaaagc ttcccgcgat
     2161 cgatgaaggg cccacgatcg ttgttgcagg ttatcctaat gtgggaaagt caagtttcgt
     2221 ggctcttgtg agcagcgccc atcctgagat cgcatcctat cctttcacaa caaaagggct
     2281 ggctgtgggg catttcacca ggaataaggt acggcatcag gtgatcgata cccccggact
     2341 tcttgatagg ccgcttgaag agagaaatga aattgagctt caggcaataa ctgccttaaa
     2401 tcatgtggga aaggtaatat tgtatatcat tgacccatcc gagacatgcg gttattctct
     2461 tgatacccag ctgcatcttc ttgatgaaat aaaaaacagg tttactgcgc catttctaat
     2521 agttgcgaac aagaaggata tttcggagac ggaaattggc gatatgagta tgtcaacgtt
     2581 aaaaggggag ggagtagatg ctgtgcttga gcgtctgctg gaaatgatgc cgataacgga
     2641 taccctggta agtgatgaaa tagcttgatt ggattttatc taatttttca ttaacataat
     2701 gtatttatcc tataaagata aaatatgaat gtttcttctg aacagaggag acttgtggga
     2761 aatcgggtta ggaaaacaac aaatttttaa gaatagctgt tgctatttat gatgtcaact
     2821 acctgtttta ctggcaaaaa aacgataatg acaaatggag gaattacatg ttgagaaaaa
     2881 gaatatggag ttgcatggtg gctctcatct tgttggtcgc ttttgcatca gtaacaactg
     2941 ctcacggatt gagtgagaaa gaaaaattgg gaaaggacct tttctttgac aaagatctat
     3001 ccgatcctgc cggtcaggcg tgcgctgcgt gtcacgcacc tgaagtagga tggactggtc
     3061 ctgacaagaa tataaatgct catggtgcag tctatgaagg tgcagtgaaa ggaagatttg
     3121 gaaacagaaa accgccatct gcagcttatg caggcgatag tcccattctg actaatacca
     3181 caccatttac aggtggtatg ttctgggatg gtagagcaac cggctggaca cttggagatc
     3241 cattagctga acaggcacag ggacctttcc tgaatccgct ggaacagaac aatcccaatg
     3301 cttcagcagt tgtcaataaa gttaaggcat catcctatgt caggctgttc tataatatct
     3361 gtggaaaagg taagactgat gctgattact acgtatgtat cgcaaggtct attgcagcat
     3421 atgagagatc ttcagaagtg agttccttca cctccaaata cgattattat ttgaaaggtg
     3481 gagctatttt gactatgaca gagaagagag gactcaaatt gtttacaggg aaaggcaatt
     3541 gtagtaattg tcatgtaagt acagggtcaa aacctttatt tacagacttc acttatgaca
     3601 acctgggagt accaaaaaat ttagagaatc cattctataa cgaacctgca tataatccaa
     3661 tgggatccgc atggattgat gaaggattgg gaggtttcct caaaggtatt ggttatccat
     3721 catatggtga agttggcaag atgaaagtcc ccacgctgcg caacgtggat aagagaccat
     3781 ataatggttt tgtgaaggca tatggacata atggctactt caagtctctc gaagaaatcg
     3841 tacatttcta taacacaaga gatgtaccag gagctggctg gaaaggtaag ccctggccgg
     3901 cagctgaagt atccgctaat gtaaacaata ttgaactggg taatcttggt ttgacacatc
     3961 aggaagagtc agacatagta gcattcctga aaactctctc tgatggatac accccaacaa
     4021 aataaagatt ttgaaaaaat cgagtccgtg tggcttcttc acacagactc atactttttt
     4081 tttaataaac acaatgtttt tatccttaaa agataagata tgaatgtgtc ttctgaacag
     4141 agaagactcg tgggaaagcg ggtttggaaa acaacatatt ttcaagaatt actgttgcta
     4201 attatgatgt ctactactgg ttttccctgc aaaaacccaa taaactataa taggaggaaa
     4261 tcaacgatat gttaagtaaa agaatatgga tttgtgcaat aatcctgctg ctgaccgccg
     4321 caactgcagc agcattaaat gatacggaac aattaggaaa gaatatattt ttccacaagg
     4381 aattatcaat cgataagaac caggcctgcg ccgcctgtca ttcaccgcaa gttggattta
     4441 cgggggggga ttctataata aatgtaatgg gatcagttta caaaggatct attgccggaa
     4501 ggttcgggaa tcgaaaacct cccgcagcgg cttatgcag
//
LOCUS       DATGBN010000446        21392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_385625, whole genome shotgun sequence.
ACCESSION   DATGBN010000446 DATGBN010000000
VERSION     DATGBN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 21392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21392
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385625"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VKL21_11225"
     CDS             complement(<1..331)
                     /locus_tag="VKL21_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46379.1"
                     /translation="MKIQPGKKGLLINLLSLIVFALLWQLLVILARKDSFNIPYIRLR
                     DIPTPVETWVALVGSMFTQLHGPGFTYGIINHVYASLQRVIEGGIIAFIIGVPVGIGV
                     GYSKLIRN"
     gene            complement(331..1431)
                     /locus_tag="VKL21_11230"
     CDS             complement(331..1431)
                     /locus_tag="VKL21_11230"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMB46380.1"
                     /translation="MKNSLKILFVIFIAIFVSGCLESKKTPEASEPINLVKMSGQEMV
                     QRLGTGEIAGFIMWEPYPAIAVTKGYGKNFIFSGKIWAEHPCCVVAYDEDWYKKTNNS
                     DEILKRMALVQLRSVEYINNAKQSDSPDHDQLINYTMQFGGMTDPVAANLSLADVEFV
                     YNTNVADTTTFIQRIQDFKIFDMAKWNQSGYKDASDYANSLVTNNYVDWAVKNNNENL
                     SKLSLKEPVNIRYAYLVNDIHELPFYVGWQKGYYKDLGINIVVAEGAPFQNGAFEMQN
                     GFKANTVDVGSLGIPPVIIHRINSNDFSKDDARVGVIAGMNNEGSVIVVANNITSIKD
                     LQGKTVGFPGPGTIQHVLFLMEAEKEGLTVSY"
     gene            complement(1588..3066)
                     /gene="secY"
                     /locus_tag="VKL21_11235"
     CDS             complement(1588..3066)
                     /gene="secY"
                     /locus_tag="VKL21_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMB46381.1"
                     /translation="MAFESLAPLLNRLPAVKRPEGHVHFKKKLGWTLGILVLYFVLAN
                     IPLFGLDKSSIDLFELYRAFFAGASGSLMVLGIGPIVTASIVLQLLVGANVIRMDLTD
                     PHQQAIFQGLQKLLVFVMIVLEALPQILGGYMKADAGLAATLGVDVSFVTLLLFIQVC
                     AGGILILYMDEIVSKWGIGSGVGMFIIAGVSQQIVQGLFNWKTDNTGVPIGIFPKWIF
                     MAGPNSGIDMNYILTNKVLFLYEAGILALFSTILIYLMVVYVESTRIEIPLAHAAVRG
                     ARGRFPVKLIYASVLPMILVRALQANIQMVGLILSTKGVTMLGTYQKGTPVGGLMYYL
                     SPINGPSDWIPSLVGPKFTSLGVAEPAIWKIILHVFTDASMLIIGGIVFALFWIETTG
                     MGAKNVAEKIQKSGMQIPGYRRSSGTLERVMQRYIPKVTVIGGAFIGILTLIASLLGT
                     LGGAGGTGLLLTVSIMYRLYEDIASQQMMEMHPMMRSFFGKD"
     gene            complement(3073..3531)
                     /locus_tag="VKL21_11240"
     CDS             complement(3073..3531)
                     /locus_tag="VKL21_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HMB46382.1"
                     /translation="MSKTKTKKFRGSRTCGGGTHKNRRGGGSRGGRGNAGTCKHHFVR
                     SMQRGLSFGKHGFKRPQSVTSDLIIVNIGEIDEAIEQLVTDGFAEKKGNAFHIDLGNI
                     GIEKVLGSGKVTKTLFITASEFSSLAKQKIEEAKGQTINPAEKSESTKQE"
     gene            complement(3537..3998)
                     /locus_tag="VKL21_11245"
     CDS             complement(3537..3998)
                     /locus_tag="VKL21_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMB46383.1"
                     /translation="MFAIVKLRGEVKTRQDIRDTLRMLRLDRVNHCVVVPETPTYSGM
                     IHKAKDYVAWGVITPETLEEMLENRGKLEGGDDLTEEYLSENTKFKSFKELAKAICEG
                     SSAIADIPKLKPVFRMHPARKGLKGTKRTFQEGGDLGFHGAEINTLLTKMR"
     gene            complement(4002..4622)
                     /locus_tag="VKL21_11250"
     CDS             complement(4002..4622)
                     /locus_tag="VKL21_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMB46384.1"
                     /translation="MRDKGFEQHKEEWFPQTRLGKLVKEGQVTTMGEALASGLPIRES
                     QIVDALLPEIRDEVLDINMVQRMTDSGRRVKFRATVIVGNGDGFIGIGEGKDVQVGIA
                     IRKAIDTAKMNIIGIKRGCGSWECGCGQGHTVPFEVKGKTGSVEVKLRPAPRGLGIAS
                     GGTAKKVLEIAGIKDVWTKVSGETRTTLNFAKATFDALIKTTTMKV"
     gene            complement(4628..5146)
                     /locus_tag="VKL21_11255"
     CDS             complement(4628..5146)
                     /locus_tag="VKL21_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMB46385.1"
                     /translation="MATGSRYKVKFRRVRMGKTDYRARKQLLISKKPRLVIRKSLKNT
                     QVQIIVPQSAGDVTLVCANTQDLKKYGYTATTGNITSAYLAGLLLGYRAKKKGQTVAI
                     TDLGLYHTTKGGLLFAALKGAIDAGLEIPHDPKIFPSEDRIMGKHIDKYRKTNIAEQF
                     QAVKQKIEGEFR"
     gene            complement(5149..5601)
                     /locus_tag="VKL21_11260"
     CDS             complement(5149..5601)
                     /locus_tag="VKL21_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HMB46386.1"
                     /translation="MTDLANQRRLAAEVMSIGVNRVWMNPEAYKDISTALTREDIRKL
                     ISEEKIGKRNIQGVSRGRARKVDETRAYGHRKGHGSRSGAKGARNPKKEQWMKKIRAL
                     RSQLKEMKENKTIEVSTYRKLYRKAKGGEYRSRAHLIAHVEQLKAKEA"
     gene            complement(5603..6250)
                     /locus_tag="VKL21_11265"
     CDS             complement(5603..6250)
                     /locus_tag="VKL21_11265"
                     /inference="COORDINATES: protein motif:HMM:NF006332.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HMB46387.1"
                     /translation="MENEAIKQLTTIEGVGKAKAKILYDAGFKTIGSVKKANENELAG
                     IKGIGEKLAQKIKVSADQMVIEEENIEEQAEIIETSPITITLDAETKRLLNVRKKQKS
                     KKPTFKQTDSHKKKKLADHWRKPDGIHNKTRYSKHGKCPLVEAGYGSPAAVRGLHPSG
                     FEEIIVNNIKEVESLSVDRQAGRIARTVGARKRSLIEKKAAELGLKILNPTRKVD"
     gene            complement(6256..6786)
                     /locus_tag="VKL21_11270"
     CDS             complement(6256..6786)
                     /locus_tag="VKL21_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMB46388.1"
                     /translation="METKRTIEIPEGVNINIDSTNVSISGPQGQLQKKLWYPGVAIKK
                     ADSELIVETNIPRREQLAMIGTYESHLKNMIAGVTKGYEYTMKVVYSHFPIQIKTEGN
                     KVIIANFLGEKKSRKANILGNTKVTIKGDQVILNGINKEDVGQTAANIRQATKIKRFD
                     PRVFQDGIYLVEQSGR"
     gene            complement(6796..7185)
                     /locus_tag="VKL21_11275"
     CDS             complement(6796..7185)
                     /locus_tag="VKL21_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMB46389.1"
                     /translation="MLLDPLADSLSTIKNAEMIGKSDCTIKPASKLIGNVLKVMNDKG
                     YIGDFEFVDDGKSGYFKVQLKGKINKCGVIRPRHAVKNTDFEKWEKRYLPAKGFGSII
                     LTTPDGVMTHSEARDNGIGGELLAYVY"
     gene            complement(7195..7347)
                     /locus_tag="VKL21_11280"
     CDS             complement(7195..7347)
                     /locus_tag="VKL21_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HMB46390.1"
                     /translation="MERSNKKFGAGANACKRCGRKQGLVRKYGLYLCRQCFRDTAYQM
                     GFEKYT"
     gene            complement(7348..7845)
                     /locus_tag="VKL21_11285"
     CDS             complement(7348..7845)
                     /locus_tag="VKL21_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HMB46391.1"
                     /translation="MRTPSIDKVTVHICTGESGERLTNAEKLLGTIVKQKPVRSMAKK
                     TLPNFSIKKNEPIGCKVTLRGNNAREFLKIALKIIDNKISATQFDQNGNFSFGVEEHT
                     DFPGMKYDPSIGIYGMDINVAIKRAGYRIRARKIEKNKISNDHRLKKEDVISFVKDKY
                     SVEVI"
     gene            complement(7855..8547)
                     /locus_tag="VKL21_11290"
     CDS             complement(7855..8547)
                     /locus_tag="VKL21_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HMB46392.1"
                     /translation="MATHQKRVAAPISWPITRKTHHWVVGANPGAHSMESGIPLLVVV
                     RDILKIANNAREAKRIINEKNIHIDGVARTDYKHMVGLFDIVSIPATNEYYRVLLDTK
                     NKFKLFKEDANAGKLCRINNKTIVRKGAVQLNLHDGTNVLGSNDYKTFDTIILDTKDH
                     KIIKHLGYKAGNLAMIVGGEHSGEIGKIKQIRKVRGSGTNMVAISNEREFETIEDYVY
                     VIGETTPQIKVV"
     gene            complement(8548..8889)
                     /gene="rplX"
                     /locus_tag="VKL21_11295"
     CDS             complement(8548..8889)
                     /gene="rplX"
                     /locus_tag="VKL21_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HMB46393.1"
                     /translation="MVSTQPRKQRKSRYQAPLHIRHKFMGAMLSPVLREKHKIKSIPI
                     RAGDTVKVLRGDNKGKEGKVAVVNLANMTISIDGVSVTKSDGTEVPRPIQPSNVMITK
                     LETKDEKRLGD"
     gene            complement(8899..9297)
                     /locus_tag="VKL21_11300"
     CDS             complement(8899..9297)
                     /locus_tag="VKL21_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HMB46394.1"
                     /translation="MKGMKAKIPRAINTAARMECADNTGARIVEVISVLKYRGVRNRY
                     PRGGVGDTLIVSVKKGTPETRKQVFKAVIIRQKKEFRRPDGMRVSFEDNAVVIVDDEG
                     VPKGTEIKGPVAREAAERYSKISSAASIIV"
     gene            complement(9294..9617)
                     /locus_tag="VKL21_11305"
     CDS             complement(9294..9617)
                     /locus_tag="VKL21_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HMB46395.1"
                     /translation="MTRDIGLDVQPPTKECADPNCPFHGTLPVRGQVLSGVVVSDKMD
                     KTVVVKRTYVKKNTKYERYEKRKTKVHAHNPPCMNAAQGAKVTIAECRPLSKTKSYVI
                     IEVLK"
     gene            complement(9631..9942)
                     /locus_tag="VKL21_11310"
     CDS             complement(9631..9942)
                     /locus_tag="VKL21_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 1"
                     /protein_id="HMB46396.1"
                     /translation="MKMTPQNIIHHELIGLDTKVVSSTNSSLIGIEGRIVDETKNVLI
                     IETDVQEKKIPKSCSSFIFTVPPVDGKRYLPLTLKVDGRLLLSQPENRIQTKFKKKFR
                     K"
     gene            complement(9939..10142)
                     /gene="rpmC"
                     /locus_tag="VKL21_11315"
     CDS             complement(9939..10142)
                     /gene="rpmC"
                     /locus_tag="VKL21_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HMB46397.1"
                     /translation="MAILRSDEIRKMNTNERQEELDKILMELLRERAIASAGGAPESP
                     GKMKEIKRTIARIKTIQTEKNKE"
     gene            complement(10144..10986)
                     /locus_tag="VKL21_11320"
     CDS             complement(10144..10986)
                     /locus_tag="VKL21_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HMB46398.1"
                     /translation="MGVEKKFVKNGFDKTQMAEYFSKQLERAGYGGMNINRTPMGTQV
                     TIFAEKPGMIIGKGGRTIHKLTHDLETVFRVDNPQIDVQEVKIPELNAQMMASRLAGA
                     IERGLYFRKAGHNMLRRIMESGALGCEIDIAGKLTGPRKRTEKFVAGNMLHAGNPAME
                     LVDKGFAIAIKKLGVIGCRVRLIRPTVKLPGRFKTVDIIVPQEAAPSGKKPEGIKELV
                     KAAPVETRPEIIKPEPEIKKEILPEEIHTGDIEERMHADVLEHKHQEYDYWHPASRTH
                     KTEE"
     gene            complement(10986..11432)
                     /locus_tag="VKL21_11325"
     CDS             complement(10986..11432)
                     /locus_tag="VKL21_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HMB46399.1"
                     /translation="MKLNFSIEPSPEKTSKAMGKELHISRKQAHEIATAIKGMKLDIA
                     REFLENVAALKQAVPYKRYTRNIPHRRGMCSGRYPQKAAKEILCILKNAESNATYKGL
                     DSGTMRITHIATKKGHSYMGQFPRAQGRATPKRQETVTVEMIAEVQ"
     gene            complement(11438..11851)
                     /locus_tag="VKL21_11330"
     CDS             complement(11438..11851)
                     /locus_tag="VKL21_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HMB46400.1"
                     /translation="MARKESSKIPKRKGEFTFRGKSVEDLKKLSYDEFALLVPSRQRR
                     TIQRGFSEDHKKLLHKVKIKDQNIRTHLRDMIVLPEMVGMKIAIHSGKEFVPIDILPE
                     MMGHYFGEFVLTRRKVSHGAAGIGATKSSKFVPLK"
     gene            complement(11857..12567)
                     /locus_tag="VKL21_11335"
     CDS             complement(11857..12567)
                     /locus_tag="VKL21_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HMB46401.1"
                     /translation="MGKRIMSQSRGKGSPTYKAPSHKFKANLEHLKVQEGIITGKVLE
                     ITHDPARSAPIARISFSNGEERLMLIPEGTEVNQTIQCGISAPVEPGNTLVLAEIPEG
                     VPICNVESQPGDGGKFARASGMYGILVAHDVGKTVVQLPSGEMKWLNPKCRATIGVVA
                     GSGRTEKHFVKAGKKFFRMKVRARKWPRSRGVAMNVVDHPFGGGGRQHPGRPKTVARG
                     TPPGRKVGSIAARRTGKR"
     gene            complement(12577..12822)
                     /locus_tag="VKL21_11340"
     CDS             complement(12577..12822)
                     /locus_tag="VKL21_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HMB46402.1"
                     /translation="MIQHPYVTEKATMMAENNNVLQFIVDINDSRTNVKREIEALFKV
                     KVANVNMMMTSKGKKKATVSFVDPDTATELASRLGIF"
     gene            complement(12819..13571)
                     /gene="rpl4p"
                     /locus_tag="VKL21_11345"
     CDS             complement(12819..13571)
                     /gene="rpl4p"
                     /locus_tag="VKL21_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HMB46403.1"
                     /translation="MDLNIIDLSGNSTKKITSTLFDEPYRPDLIKKAVLAAQANRMQP
                     YGPHMYAGMRTSAEGWGPGRGVSRVARIMNGSRAARIPQAVKGRQAHPPKPETDRTEK
                     INDKERKKAIRSALAATGNVDLVKKRGHQFSASLPLVVADDFSALTKTKDVKSFLEAA
                     KLWDDILRAKNTRVRAGKGKLRGRRYKQPKSILIVTAEDKGVLKAARNLAGVDVVNYD
                     QLNAELLAPGTHAGRLTIFTESAILKLEEKMQ"
     gene            complement(13576..14589)
                     /locus_tag="VKL21_11350"
     CDS             complement(13576..14589)
                     /locus_tag="VKL21_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HMB46404.1"
                     /translation="MSHPHRPRRGSIGFSPRVRARSEVPRVRAWPVQKEPKLLGFAGY
                     KAGMTHLIMRDEIPNSLTNGMEISVPVTIMEVPAMKVAAIRLYKNTTYGANAIAEAWT
                     TELDKELNRAIPVPKKHDLPASIARIEQLIKDGIAKDLRVVMYTMPEKVTGIPKKKPE
                     IMENNIGGTDMMARFQYAKGLLGKNINISDVFNNGDVIDLVGITIGKGVQGPVKRWGI
                     QLQKNKHSRAGSVREIGTLGAWHPSHVSWRVPQLGQTGYHQRTEFNKRIMQMGKDGKN
                     VTPEGGFLNYGIVRNDYVIIKGSVPGPVKRLVRMRPAIRPRKQTKGQAPEITYISLES
                     KQG"
     gene            14938..15009
                     /locus_tag="VKL21_11355"
     tRNA            14938..15009
                     /locus_tag="VKL21_11355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14971..14973,aa:Gly,seq:tcc)
     gene            complement(15101..15382)
                     /locus_tag="VKL21_11360"
     CDS             complement(15101..15382)
                     /locus_tag="VKL21_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46405.1"
                     /translation="MNVIYKITYPNGKIYIGQDRTDDIRYFGSPNRDLIENEFTRDQK
                     SDFTLRKEIVWESKTASIEEVYLKEIEFIKKFRSNDPVIGYNRWPKFNK"
     gene            complement(15417..15767)
                     /locus_tag="VKL21_11365"
     CDS             complement(15417..15767)
                     /locus_tag="VKL21_11365"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HMB46406.1"
                     /translation="MGKSGDEGIDGIIKEDKLGLDTIYIQAKRWDGTVGRPEIQKFIG
                     ALQMKGAKKGIFITASNFSKEARESVDMTGSTKIVLIDGELLVNLMIQNDIGVSKIAS
                     YEIKQIDSDYFSEE"
     gene            complement(15798..16331)
                     /locus_tag="VKL21_11370"
     CDS             complement(15798..16331)
                     /locus_tag="VKL21_11370"
                     /inference="COORDINATES: protein motif:HMM:NF025696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMB46407.1"
                     /translation="MAIPDYQSIMLPLLKFAGDKKEHSIREAIEHISGLFNLSNEEKR
                     ELLSSGQQPIINNRTGWARTYLKKAGLLESPRKSYFKITDIGLNVLKQNPPEITNKYL
                     EQFPGFVEFKTIKKPDVEIKSLIQYPQAEGTPRESIEENILKLQNDLGGDLIIKIKQS
                     SPEFFERLVIDLLLKMG"
     gene            16557..17969
                     /locus_tag="VKL21_11375"
     CDS             16557..17969
                     /locus_tag="VKL21_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMB46408.1"
                     /translation="MNKLLRVLIVEDSKFDARVLIGELENGGYRLDHELVDNPEDMRA
                     ALGKKPWDIVISDFVMPNFSGMDAIRILQESGLDIPIIIVSGKIGEDTAVESMKAGAH
                     DYIIKGNLARLIPAIERELFEAEERRKRKRVEAELKSTKEKLESFINNTSDAIIIIDM
                     EKKILQVNKGFEKLYGWTATEAIGIQLPIIPDNLMPETKKLLAEVESGKVITAHETIR
                     RRKDKTLFDVSVTISPIKDASGKVVAFAGISRDITERKRAEEQTMCSLLEKETLLREI
                     HHRVKNNMQVISSLLRLQSKYIKDKDDLEIFKDSQNRISSMSLVHEKLYQSNDFTKID
                     FSVYVNDLVKSLFHSYGAISNNIIPNIKVNNVSLGIDSAIPCGLIINELVTNSLKHAF
                     PDGRKGEIRVFMGQTGEKEFELVIRDNGVGIAQGIDFDKSDTLGLHLVRILAENQLHG
                     EINLIRNHGSEFKINFKGAK"
     gene            17969..18790
                     /locus_tag="VKL21_11380"
     CDS             17969..18790
                     /locus_tag="VKL21_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB46409.1"
                     /translation="MSKKQILIVEDESIVAEDLRQSLIGLGYAVPSVESSGEKAIEKI
                     MEKRPDLVLMDIVLHGEMDGIDAAKEIHDRFDIPVVYLTSYSDEKILDRAKITEPYCY
                     IIKPFKERELHINIEIATYKHKMEKKLKESKQWLDSTLKSLGEAVISTDKEGFIKTMN
                     PIAEAMTGWKSDEAASKPISTVFNIISEKPNVNVENPVTKAIRVGMFYGLAEHTVLVT
                     KEGLKVPVDIIGSVIKDDLGNIMGIVMIFEDIIERKRMEERLNKANCLSVNNDQK"
     gene            complement(18804..19367)
                     /locus_tag="VKL21_11385"
     CDS             complement(18804..19367)
                     /locus_tag="VKL21_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HMB46410.1"
                     /translation="MLIALTGTPGTGKTSVCEVLKEHSKYRKLYSIIDLNKLVLDEKL
                     YTGKDEVRDTYEVDIDKLEERLKHEIEKKSGDVIMEGHLCHLLPADAVIVLRAHPVAL
                     RKRLGKRKNYSFQKVKENADAEALDVILVEATQRNKSVFEINTTNMNIIAVVKSVISV
                     IEALKLGKTPEDFLPGKIHWIEQVTMD"
     gene            complement(19361..20455)
                     /gene="hisC"
                     /locus_tag="VKL21_11390"
     CDS             complement(19361..20455)
                     /gene="hisC"
                     /locus_tag="VKL21_11390"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMB46411.1"
                     /translation="MRFKELVRPSVKDIKEYVPGKSIEEIAGKYGLDPDSIIKLGSNE
                     NPLGPSPRAVAAIKEKAGRVHLYPQADALVLRKAIGEYTGYPSSNIVVSGNGMDGIID
                     TIMKLFMSQGTESIIPIPTFSYYEIATLANGGKPVFVERDSDYYIAPENILDKANVNT
                     RMIFLCSPNNPTGNSISEKDARKILESTDAIVFIDEAYVEFANSSITGLVREYENLIV
                     GRTFSKAFGLAGMRMGYAIAPEWIVREYMKVMTPFSMDILSTAAGLAALGDKKHLNKS
                     VEAVKKGRMQLSKGVGTLCKVYSSDANFILVDVSPNSSKEVSELLLKKGIIVRDCTSF
                     RGAGKSLIRISVGMQEQNEKVIEAFREILC"
     gene            complement(20445..>21392)
                     /locus_tag="VKL21_11395"
     CDS             complement(20445..>21392)
                     /locus_tag="VKL21_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HMB46412.1"
                     /translation="EQLIHVSNLYYTENQALLAEELASISGMERVFYCNSGAEAVEGA
                     LKLARKASGKKEFIATEHAFHGRTRGALSVTHKEKYRKPFEPLAPAVFVPYNDTEAIR
                     SAITENTAGLIIEPIQGEGGVIIPSDDYLKEVREICDDKSVLLILDEVQTGFGRTGKW
                     FAREHSGIIPDIMTTAKAMGGGFPMGAMLAGEDVAANFVRGDHASTFGGSALACAAAL
                     ANIDVIKKEKLVKRSYDMGNYLVSKLRQLNKDYILEIRGKGLMVGMELSIKCEDIVAG
                     AREKGVLLNCTSESVLRFVPPLTITEQQLATVVSVLNEI"
BASE COUNT         5325 a         4955 c         4052 g         7060 t
ORIGIN      
        1 tgtttctgat aagctttgaa tatccgacac cgatacccac agggacgcca atgataaatg
       61 caattatacc gccttcgatc acgcgttgca aactggcata aacatggttg atgataccat
      121 aagtgaaacc gggcccatgc agctgggtga acatcgaacc aacaagcgcg acccacgttt
      181 caacaggcgt gggaatgtcc cgcaggcgaa tataaggaat attgaaacta tctttcctgg
      241 caagtatcac aagcagctgc cataacagcg caaaaacgat aagggaaagg aggttaatta
      301 ataatccttt tttccctggc tggattttca ttaatagctg accgtaagtc cttccttttc
      361 tgcctccatg aggaaaagaa catgctgtat agtaccgggt ccggggaatc caactgtctt
      421 tccctgtaag tctttaatag aggtaatgtt attggcgaca acgatcaccg acccttcatt
      481 attcatccct gcgatcactc caacgcgtgc gtcatctttg gaaaaatcat tgctattgat
      541 ccggtgaata attacaggag gtatgccaag acttccaaca tcaacggtat tggctttaaa
      601 gccgttttgc atctcaaaag ccccgttctg gaatggagcg ccttcagcta caacaatatt
      661 tattcccaaa tctttataat atcctttttg ccatcctaca tagaatggta gttcgtgaat
      721 atcatttaca aggtaagcat accggatgtt gacaggttct ttcaatgaaa gctttgagag
      781 attttcattg ttgtttttga ctgcccaatc cacataattg tttgttacga gtgaatttgc
      841 ataatccgaa gcgtccttat atcctgattg attccatttc gccatgtcga atatcttaaa
      901 atcctgtata cgctgaataa atgtcgttgt gtcggcaaca ttagtattat aaacaaattc
      961 cacatcggca agactcaggt tcgcagcaac cggatctgtc atccctccaa actgcattgt
     1021 ataatttatc aattggtcat ggtcgggtga atcgctctgc tttgcattat tgatatattc
     1081 aacggatctt aactgcacaa gcgccatgcg cttgaggatc tcatcagaat tattggtctt
     1141 cttataccag tcctcatcat acgctaccac acagcaggga tgttcagccc atatttttcc
     1201 tgaaaatata aagtttttcc catacccttt ggtaacagct attgcaggat aaggttccca
     1261 cataatgaat cccgcaattt ccccggtccc caatctctgt accatctcct gcccgctcat
     1321 tttcacaagg tttattggtt ctgatgcttc aggagttttt ttgctttcaa gacatccgga
     1381 tacaaagatt gcaataaaaa ttacaaacaa gatcttcagt gaatttttca taattttcct
     1441 caaacagttc tatacattat agatttaaat tacgatattt aatagttccg tttttatctg
     1501 aacctctcca tgcttttgcg atattcagaa catgaaaaca gggtgactcc cgaaaaggag
     1561 ttccttgttt gtcaatttac tttgttttta atctttcccg aagaagcttc tcatcatggg
     1621 gtgcatttcc atcatctgct ggcttgctat atcttcatac aacctgtaca tgatacttac
     1681 tgtcagcaat agacctgttc cgcctgcgcc gccaagagtt cccagaagac ttgcaatgag
     1741 tgtaagaata cctatgaatg ccccgccgat cactgttact ttggggatat accgctgcat
     1801 cacacgctca agcgttccgc tgcttctgcg ataacctggg atctgcattc ctgatttctg
     1861 gatcttttcc gctacgttct ttgcgcccat tcctgtggtt tcaatccaga aaagagcaaa
     1921 gactataccg ccaataatga gcatggacgc atccgtgaaa acatgaagga ttattttcca
     1981 gatcgcaggt tctgcaaccc caagacttgt gaattttggc ccaaccagag atggtatcca
     2041 gtcactcggt ccgtttatcg gtgatagata atacattaga ccaccgacag gcgttccttt
     2101 ctggtaagtt cccaacatcg ttacaccttt ggttgaaagg atgagaccta ccatctggat
     2161 atttgcctga agagccctta ccaggatcat tggcaaaaca cttgcataga tgagttttac
     2221 cggaaatcgc ccgcgtgccc ctcttacagc cgcatgggca aggggtatct cgatacgtgt
     2281 actttctacg tagacaacca tcagatagat taatattgta ctgaacaaag ccaggatacc
     2341 cgcttcgtag aggaatagta ccttgttggt caggatgtaa ttcatatcaa taccggagtt
     2401 aggacctgcc ataaatatcc atttaggaaa gatacctatg gggactcccg tattatctgt
     2461 cttccaattg aatagaccct ggactatctg ttgcgataca ccggcgataa taaacattcc
     2521 aacacctgag cctatacccc atttggagac tatttcgtcc atatagagga tcaatatacc
     2581 gccggcgcat acctgaatga acaacagcaa tgttacaaaa ctaacatcaa cgccaagcgt
     2641 cgctgcaagc ccagcatctg ctttcatgta gccgccaagt atctgcggca gggcctcaag
     2701 aactatcatt acaaaaacaa gaagtttctg caaaccctgg aatatggcct gttgatgcgg
     2761 atcagtgaga tccattctaa tgacatttgc ccctacgagc aattgcagca ctattgaagc
     2821 cgtgactatc ggacctattc ccaggaccat cagggaacct gaagctcccg cgaagaatgc
     2881 cctgtacagt tcgaaaaggt caatcgatga tttatccagt ccgaatagcg ggatattggc
     2941 caaaacgaaa tacaggacaa gaatacccaa tgtccatcca agttttttct taaaatgcac
     3001 atgaccttcc ggacgtttga ccgcaggaag gcggttcaat aggggtgcaa gactttcaaa
     3061 tgccatgttt tattactctt gttttgtcga ttctgatttt tctgccggat taattgtctg
     3121 ccctttggct tcttcgattt tctgctttgc cagagatgag aactcggaag cggttatgaa
     3181 taaggttttc gtcactttcc cactcccaag tactttttcg ataccgatat tgcccaggtc
     3241 aatatggaat gcattgcctt ttttttccgc aaatccatct gttacaagct gttcgattgc
     3301 ctcatctatc tctccgatat tcacaatgat caaatctgat gttacagact gtgggcgttt
     3361 gaacccgtgc ttaccaaaac ttaagccacg ctgcattgaa cggacaaaat gatgcttgca
     3421 tgttccggca tttccgcgtc caccgcggct gcctccacct cttcggttct tatgcgtgcc
     3481 cccgccacat gtccttgatc ccctgaattt cttggttttt gttttgctca tgataattac
     3541 ctcatcttag ttaacagggt attaatttca gctccatgga atccgagatc tcctccttcc
     3601 tgaaaggttc tttttgtacc ctttagacct ttccttgccg gatgcatcct gaaaacaggt
     3661 ttcaatttag gaatatcagc tattgctgag cttccttcac atatggcttt tgccagttcc
     3721 ttgaaggatt tgaatttggt gttctcactc aggtactctt cagtcaggtc atccccgcct
     3781 tcgagtttgc ctctattttc aagcatttct tcaagtgttt ccggggttat gacgccccac
     3841 gcaacgtaat cctttgcctt gtggatcatc ccactatatg tgggtgtctc aggtacgacc
     3901 acacagtgat ttaccctgtc aaggcgcaac attcttaatg tatccctgat atcctgcctg
     3961 gtttttactt cccctcgaag ttttactata gcgaacattt ttcacacctt catagttgtt
     4021 gtttttataa gtgcatcaaa agttgctttt gcgaaattaa gggtggttct ggtctccccg
     4081 cttacttttg tccagacatc tttgattcct gcgatctcca gcaccttttt tgcagttccg
     4141 cctgaagcaa ttccaagccc ccggggcgca ggcctgagtt taacttccac acttccggtt
     4201 ttccctttta cttcaaaagg aacggtatga ccctgtccac agccgcattc ccatgaaccg
     4261 cagccgcgtt taatgccgat aatgttcatc ttggcagtgt ctatagcttt cctgatagcg
     4321 attccaacct ggacatcctt gccctctccg atgccgataa aaccatcacc attaccaacg
     4381 atgaccgttg ccctgaattt tactcttctt cctgagtctg tcattctttg aaccatgttg
     4441 atatccagaa cttcatctct gatctctggc aataacgcat ctacgatctg agattccctt
     4501 atcggtaatc ccgatgcaag ggcttcaccc atggttgtaa cctgtccctc cttgaccagt
     4561 tttcccagtc ttgtctgagg gaaccattcc tctttatgtt gttcaaatcc tttatctctc
     4621 attttgttca cctgaattcg ccctcgatct tttgctttac tgcctggaat tgttctgcaa
     4681 tattggtttt cctgtatttg tcaatatgct tgcccatgat cctgtcttcg gacgggaata
     4741 tcttggggtc gtgcggtatt tcaagacctg cgtctatcgc accttttaat gcggcaaaca
     4801 aaagtcctcc ttttgtagta tgatataatc ccaggtcagt tattgccact gtctgtcctt
     4861 ttttctttgc cctatatcca agcaataatc ctgccagata tgctgatgtg atatttccag
     4921 tcgtggcagt gtagccatat ttctttaaat cctgcgtatt ggcgcatacc agtgtaacgt
     4981 ccccggctga ttgaggtact attatctgta cctgtgtatt tttcaggctt ttcctgatta
     5041 cgagcctggg cttttttgat atcagcaatt gctttcttgc acgataatcc gttttgccca
     5101 tcctgactct tctgaatttt actttgtaac gcgatcctgt tgccatattc aagcctcctt
     5161 ggcttttagc tgttccacat gggcaataag atgtgccctg cttctgtatt ctccaccttt
     5221 ggcctttcgg tataatttcc tgtaagttga gacttcaatg gtcttgttct ctttcatttc
     5281 cttaagttga ctgcgcaatg cacggatctt tttcatccat tgttctttct taggattcct
     5341 tgcgcctttt gctcccgatc ttgaaccatg ccccttcctg tgcccatatg cacgggtttc
     5401 atcaactttc cgggctctac ctcggcttac tccctggata tttctttttc cgatcttctc
     5461 ttcgcttatc aattttcgga tatcttcccg cgtaagagct gtagaaatgt ctttatatgc
     5521 ttcagggttc atccatacgc ggttgacacc aattgacata acttctgccg caagtcttct
     5581 ctgatttgcc agatctgtca ttttaatcta ccttccttgt gggattcaga atctttaatc
     5641 ccaattcagc agcttttttc tcgatcaagc tgcgctttct tgctcccact gttctggcaa
     5701 tcctgccagc ttgcctgtcg acactgagag attcgacctc tttaatatta tttacaatta
     5761 tctcttcgaa tcctgaggga tggagcccgc gaacagccgc cggacttccg tatcctgctt
     5821 caacaagagg acattttcca tgctttgaat agcgtgtctt attatgtatg ccgtccggtt
     5881 ttctccaatg atccgcaagc tttttcttct tatgggaatc tgtctgcttg aatgtgggtt
     5941 tcttgctttt ctgttttttc cttacattaa gaagtctttt tgtttctgca tcaagtgtga
     6001 tagtgatagg cgaagtttca attatttccg cttgctcttc tatattctct tcttcaatca
     6061 ccatttgatc ggcagaaact ttgatcttct gggcaagttt ttcacctatt cctttgatgc
     6121 cggcaagctc attttcgttt gcttttttta cggatccaat tgtcttaaat cctgcatcat
     6181 aaagtatttt cgctttagcc ttgccaacac cctctatggt tgtaagctgt ttaatcgctt
     6241 cattttccat aattttcacc gtcccgactg ttccacaagg taaatcccat cctggaacac
     6301 cctggggtca aatcttttta tttttgtagc ctgacggata ttagctgctg tctgaccaac
     6361 atcttcctta ttgataccat taaggatgac ctgatcacct tttatcgtga ctttggtatt
     6421 gcccaggata ttggcttttc ttgacttctt ttcgccaagg aaattagcaa ttattacctt
     6481 gtttccttcc gtctttatct ggatcgggaa atgtgaataa acgaccttca tcgtatattc
     6541 gtatcctttg gtaactccag cgatcatatt ttttaagtgg gattcatatg tgccaatcat
     6601 tgcgagctgt tctcttcgtg gaatgttcgt ctccacaata agttcggaat cagccttctt
     6661 tatggcaact cccggatacc agagtttttt ctggagttga ccctgcgggc ctgatatcga
     6721 tacattcgtg ctatcgatat ttatattaac accctcaggg atttctattg ttctttttgt
     6781 ttccatgact ttcacctaat aaacataagc aagtaactct ccgccaatgc cattatccct
     6841 ggcttcactg tgggtcataa caccatcagg agttgtcagg attatagagc cgaagccctt
     6901 tgctggcaaa taccgctttt cccatttttc aaaatcggta ttcttcacgg catgtcgcgg
     6961 tctgattact ccgcacttgt tgatttttcc tttaagctgt accttgaaat aacccgattt
     7021 cccatcatcg acgaattcga aatcgccgat atacccttta tcattcatga ctttaagcac
     7081 atttcctatg agctttgaag ccggtttgat tgtgcaatcg gatttaccta tcatttctgc
     7141 attttttatt gttgataatg aatctgcaag tggatctaat aacattgaaa tcacctatgt
     7201 atatttctca aagcccatct ggtatgctgt atccctgaag cactgcctgc agaggtataa
     7261 cccatatttt cgaactagtc cctgtttcct gccgcagcgt ttgcaggcat tggcaccggc
     7321 tccaaatttt ttattacttc gttccattta aattacctcc acactgtact tatctttcac
     7381 aaaagaaata acgtcttctt tcttcaatct gtgatcgttg gatattttat ttttctcgat
     7441 ctttcttgcg cgtatcctgt atcccgctct ttttatggca acattgatat ccattccata
     7501 gatacctatt gagggatcgt atttcattcc gggaaaatct gtatgttcct ctaccccgaa
     7561 tgaaaaattc ccattttgat cgaattgtgt tgcacttatc ttattatcaa tgatcttcaa
     7621 tgcgatttta aggaactccc tggcattgtt tccccttaag gtcaccttgc agccgatggg
     7681 ttcatttttc ttgatcgaaa aattcggcaa tgttttcttt gccatggatc tgacgggttt
     7741 ttgcttgacg atagtcccga gcaatttttc tgcattggtc aatcgctcac cgctttctcc
     7801 tgtgcagata tgtacagtta ctttatcaat tgaaggtgtt ctcatcggat tcatttatac
     7861 caccttgatc tgaggtgtgg tttcacctat tacatataca taatcttcaa ttgtctcaaa
     7921 ttccctttca ttggatatgg ctaccatgtt cgttcctgaa cctctgactt ttcggatctg
     7981 ctttatcttt ccgatttcac ctgaatgttc accgcccacg atcattgcaa gattaccggc
     8041 tttgtagccc aggtgtttaa tgatcttgtg gtcctttgta tcaagaatta tggtatcaaa
     8101 agtcttataa tcatttgatc caaggacatt tgtaccatca tgcaggttga gctgtacagc
     8161 tccttttcta acaatggttt tattgtttat cctgcatagc ttcccggcgt ttgcatcttc
     8221 tttgaacagt ttaaacttgt tttttgtatc aagaagaact cgataatatt cattggtagc
     8281 cggtattgat acaatatcaa aaagacccac catgtgtttg taatcggttc ttgctacacc
     8341 gtctatatga atgttctttt cattaattat cctcttagct tccctggcgt tgtttgcgat
     8401 cttaaggatg tcccgtacta ccacaagtaa tggaatcccg ctttccattg aatgggctcc
     8461 cggattagct ccaactaccc agtgatgtgt ttttctggta ataggccacg atatgggcgc
     8521 tgctactctt ttctgatgtg ttgccattta atcacccaag cgcttttcat ctttggtttc
     8581 taattttgtt atcattacat ttgaaggttg tattggtctt ggcacctctg taccatcaga
     8641 ttttgtaaca cttacaccat ctatggaaat cgtcatattt gcaagattca ctactgctac
     8701 cttcccttct ttgcctttat tgtccccgcg caatactttg acagtatcgc ctgcacggat
     8761 aggaatactt tttattttat gtttttcacg aagtacaggg cttaacatcg cacccataaa
     8821 cttatgtctg atatggaggg gcgcctgata cctggatttt ctctgttttc ttggttgtgt
     8881 tgataccatt tttctcacct aaacaattat cgaagctgcg gatgagattt ttgagtaacg
     8941 ttccgctgct tccctggcta caggtccttt tatttccgta ccctttggta cgccttcatc
     9001 atctacgatc actacggcat tatcttcaaa agaaaccctc attccatcgg ggcgcctgaa
     9061 ttcttttttc tggcgtatga taacagcttt gaaaacctgt tttcttgttt ccggggttcc
     9121 tttcttaacg cttacgataa gagtgtctcc tacaccgccg cgaggatatc tgttccttac
     9181 accgcggtat ttcaatacag atatgacttc aacaattcgc gcgcctgtat tatccgcaca
     9241 ttccattcga gccgccgtgt ttatggcgcg cggtattttt gccttcatcc ctttcatttt
     9301 agcacctcta ttattacata ggattttgtc ttactcaagg ggcggcattc agcaatcgtt
     9361 acttttgctc cttgcgctgc attcatgcag ggggggttgt gagcgtgtac tttggtcttt
     9421 cttttctcat atcgctcata ttttgtgttc ttttttacat atgttctctt cacgactacc
     9481 gtcttatcca ttttatcgct tacaactacc ccacttaaga cctgccctct gacaggcagg
     9541 gttccatgaa atggacaatt cggatctgcg cattcttttg ttggcggttg aacatccaac
     9601 ccaatatcac gtgtcattta tatacctcat ctatttcctg aatttcttct tgaattttgt
     9661 ctggattctg ttttcgggtt gcgagagcaa taatcttccg tcgactttta gtgttaaagg
     9721 caagtaacgt ttgccgtcaa ccggcggaac tgtgaatata aaagatgaac aggatttggg
     9781 gatctttttt tcctgaacat cagtttctat gatcaaaaca ttttttgttt cgtcaactat
     9841 tcttccctct attccgataa gggagctgtt tgtgctggac actacttttg tatcaagacc
     9901 aataagttca tggtgtatga tattttgcgg tgtcatcttc attccttatt tttctccgtc
     9961 tggatcgttt tgatccttgc gatggttctt ttgatctctt tcatctttcc cggactttca
    10021 ggagcgccgc ccgctgaagc gatagcgcgt tccctgagca gctccattaa tattttgtcc
    10081 agttcctcct gtctctcatt agtattcatt ttccgtattt catcacttct caatattgcc
    10141 atactactcc tccgtcttat gggtgcgtga tgccgggtgc cagtaatcgt attcctgatg
    10201 tttatgttcc agtacatcag catgcatcct ttcttctata tcgccggtat ggatttcttc
    10261 gggaagaatt tcctttttga tttcaggttc tggttttatg atctctggcc ttgtttcaac
    10321 aggtgcggcc tttacgagtt ctttgatgcc ttccggtttc tttcctgaag gcgcagcttc
    10381 ctgtggaact ataatatcta cggtcttgaa ccttcccgga agtttaactg tggggcggat
    10441 taacctaaca cggcatccga ttactcccag tttctttata gcaatagcaa agcccttatc
    10501 aacgagttcc attgcaggat tccctgcatg aagcatattg cctgccacga atttttcagt
    10561 cctcttcctg gggcctgtca atttgccggc aatatcaatt tcacatccga gggctcctga
    10621 ttccattatt cttcttaaca tattatggcc agctttcctg aagtatagcc cgcgttcaat
    10681 tgctccggcc aaccttgatg ccatcatctg ggcattcagt tcagggattt ttacttcctg
    10741 gacatcgatt tggggattat caacacggaa aaccgtctca aggtcatgtg taagcttgtg
    10801 tatcgttctt ccaccctttc ctatgatcat tccgggtttt tctgcaaaaa tagtcacctg
    10861 ggtacccata ggggtcctgt tgatattcat gccgccgtat cctgcccttt ccaattgttt
    10921 ggaaaagtat tctgccatct gggttttatc aaatccgttc ttgacgaatt ttttctccac
    10981 gcccattatt gcacctctgc tatcatttct actgtaactg tttcctgtct ttttggcgta
    11041 gctcttccct gtgccctggg aaactgcccc atataactgt ggcctttctt tgtggcgata
    11101 tgcgtaattc tcatagttcc cgaatcaagc cctttatatg tggcattgct ttctgcgttt
    11161 ttcaatatac ataagatttc ctttgctgcc ttttgtggat acctgccgct gcacatccct
    11221 ctcctgtggg ggatatttcg tgtgtatctc ttataaggaa cagcctgttt caatgccgct
    11281 acattttcaa gaaattctcg tgcaatatca agtttcatgc cttttatggc tgttgctatc
    11341 tcatgtgcct gttttcttga aatatgaagt tctttaccca tagcctttga tgttttttca
    11401 ggcgagggtt caatagagaa atttaatttc atgtaaatca cttcaatgga acgaatttgc
    11461 ttgattttgt tgcaccgata cctgctgcgc catgcgaaac cttccttctt gtgagcacaa
    11521 attcaccgaa ataatggccc atcatctcag gaagaatatc tattggcaca aactctttac
    11581 cgctatggat ggcgattttc atgccaacca tttccggaag gactatcatg tccctcaaat
    11641 gcgtccttat attctggtcc ttgattttga ctttatgcag taatttttta tgatcctctg
    11701 aaaatccgcg ctggatggtg cggcgctgtc ttgacggaac aagcagtgcg aattcatcat
    11761 aacttaactt tttaaggtcc tctaccgatt tccctctaaa tgtgaattcg ccctttctct
    11821 ttggtatttt cgatgattct tttcttgcca tttaactcac ctctttcctg tccttcttgc
    11881 ggcgattgag ccgacttttc ttccgggcgg cgtgcccctg gctacggtct ttggtcttcc
    11941 aggatgctgg cgtcctccgc ctccgaacgg gtggtccacg acattcattg ctacacccct
    12001 gcttcttggc cattttcgtg ctctgacctt cattctgaaa aatttctttc cagccttcac
    12061 aaaatgcttt tcggtacgtc cgcttccagc gacaacaccg atggtcgccc tgcattttgg
    12121 attcaaccat ttcatctccc cgcttggtag ttgaactacg gtcttgccca catcatgtgc
    12181 aacaagtatt ccgtacatgc ctgaggcgcg ggcgaatttc ccgccatctc caggttgtga
    12241 ttccacatta cagataggca caccttccgg gatttctgct agtaccaggg tattaccagg
    12301 ttccacaggg gcggaaatgc cgcactgtat ggtctggttt acttctgtgc cttccgggat
    12361 aagcatcaag cgttcctcgc cgttgctgaa tgaaatgcgg gcaataggag ccgagcgtgc
    12421 aggatcatga gtgatttcaa gcacttttcc cgttattatt ccttcttgaa ctttcaggtg
    12481 ctcaaggttc gctttgaatt tatgagatgg cgctttatat gtaggagaac cttttcccct
    12541 actttgagac attattcttt ttcccatttt ctcaccttag aatattccta gccttgaagc
    12601 aagttctgtc gcagtgtccg ggtccacgaa actcacggtg gctttctttt ttccttttga
    12661 ggtcatcatc atattgacgt ttgccacttt tactttgaaa agcgcctcaa tctctctttt
    12721 cacatttgtt cgactatcat tgatatcaac gatgaactgg agaacattat tattttcagc
    12781 catcatcgtt gctttttctg tgacgtaggg atgctgtatc attgcatttt ctcctcgagt
    12841 tttaaaattg ccgactcggt aaatattgtc aggcgtcctg catgtgttcc aggggccagt
    12901 aattctgcat tgagctgatc ataattgact acatcaacgc ctgcaagatt gcgcgccgcc
    12961 ttcagtacac ccttatcttc cgcagtgacg atcagaatgc tttttggctg tttgtatctc
    13021 ctgccccgga gtttaccttt gcctgcgcgg actctggtat tttttgctct caggatatcg
    13081 tcccataatt tagctgcttc aaggaatgat tttacatcct ttgttttggt cagggcgcta
    13141 aaatcatctg caacaactaa aggcaatgat gcagagaact ggtgtcctct cttttttaca
    13201 aggtcaacat ttccggtcgc agcaagtgcg gagcgtatgg ctttcttgcg ctccttatca
    13261 tttatttttt cagtcctgtc ggtttcaggc tttggaggat gcgcctgtct tcctttgacg
    13321 gcctgtggga ttcttgcggc cctgcttcca ttcataattc tggccacacg tgatacgcct
    13381 cttcctggcc cccatccttc tgctgatgtt ctcattcccg catacatatg cggcccatat
    13441 ggctgcatac gattcgcctg ggccgccagt actgccttct ttataagatc aggtctgtat
    13501 ggctcatcga aaagtgttga agtaattttt ttcgtagaat ttcccgataa atcgattata
    13561 ttcaaatcca tgaaatcacc cttgtttcga ctcaaggctt atatacgtta tttcaggcgc
    13621 ctggcccttt gtttgtttcc ttggtctgat agcgggccgc atgcggacta atctcttaac
    13681 cggtcctggc acgcttccct ttattattac ataatcattt ctgacaatac cataatttaa
    13741 gaatccacct tcaggggtaa cgttttttcc atctttaccc atttgcatga tcctcttgtt
    13801 aaattcggtt ctctgatgat atcctgtttg tccaagttgc ggaactctcc agcttacatg
    13861 cgatggatgc cacgcgccaa gagtaccgat ctccctgacg cttcctgctc ttgaatgttt
    13921 gttcttctgg agctggatcc cccagcgttt tacaggtccc tgtactccct ttccgatcgt
    13981 tattccaaca agatctatga catcaccgtt attgaataca tcgctgatat tgatgttttt
    14041 cccgagaaga cctttggcat actggaaacg tgccatcata tcagtcccac cgatattgtt
    14101 ctccatgatc tcaggttttt tcttgggtat tcctgtgacc ttttcaggca tcgtgtacat
    14161 tactaccctc aaatcctttg ctatgccgtc ttttataagc tgttcgatcc tggcaataga
    14221 cgccggaagg tcgtgtttct tgggtacggg tattgcgcgg ttaagttctt tatcaagttc
    14281 agttgtccat gcttctgcga tcgcattagc cccataagtg gtatttttat atagcctgat
    14341 agctgcaact ttcatcgcag gaacttccat aatggtcacc ggtactgaaa tctccatccc
    14401 atttgttaaa ctgttgggga tttcatctct cattatgaga tgcgtcatac cagctttgta
    14461 tcctgcaaat cctaaaagtt ttggctcctt ttgtacaggc catgctctta cacgaggtac
    14521 ttcgcttctt gctctaacgc gcggactgaa tccaatagaa ccgcgtcttg gtctgtgcgg
    14581 atgtgacatt ttttcctcct tttcgataat ataaattaat acgtgggaat atttttacgg
    14641 caagtgcaaa ccttaaaaag gttttgcaaa tatgtcttca caaatagatt ttgacttaca
    14701 cgcattttac gggcatacga taaccgcatt ggccctggca attcatcaaa tccgactttg
    14761 atcgacttca acttgcgtaa tctatacgca tcgtactttc ttccatagac gaataagaag
    14821 tatataatgc tttggggatg aaaacaggaa ataacgctgt tcttcaggcc ggcgttacat
    14881 attcctggga ctttagataa agctttttat accttttgga ctatctacga actacctgca
    14941 ccgatggtct agtggctatg actttagcct tccaagctaa taactcgggt tcaaatcccg
    15001 atcggtgcat cttgcttttt taaagatgat aaagaagacc ataactggga ttttggaaag
    15061 ttttggagat tcaacagaat ttgttcgaaa gcatgcggtt tcatttatta aatttcggcc
    15121 acctattgta accaataacc ggatcatttg atctgaattt ttttataaat tcgatctcct
    15181 ttagatatac ttcctcaatg gatgccgttt tagattccca gactatttct ttccttaaag
    15241 taaaatcgct tttttgatcc ctggtgaact cattttcgat aagatcacga tttggacttc
    15301 caaaatatct tatatcatca gtcctatcct gtccaatata tattttgcca ttaggataag
    15361 ttattttgta aatcacattc atacaaaagc catcatttaa tcacagtaaa tcaaatctat
    15421 tcttcagaga aataatcaga atcaatttgt ttgatttcgt aagaagctat ctttgaaaca
    15481 ccaatatcat tttgaatcat aaggttaaca agaagttccc catcaattaa aacaatttta
    15541 gtgctccccg tcatatcaac agattctctt gcttctttag aaaaattgga agctgttata
    15601 aaaattccct tcttagctcc tttcatctgt aaagccccta tgaatttttg tatttcaggt
    15661 cgtccaactg ttccatccca tcttttcgcc tggatatata ttgtatctaa tcctaattta
    15721 tcttccttaa taatgccatc aatcccttca tctccacttt tcccgatagc ttcaccagcc
    15781 tctttttgag agccacccta tcccattttt aataataaat ctattacgag tctttcaaaa
    15841 aattctggtg aagattgttt gatttttatt atcaggtctc cacccaaatc attttgtaat
    15901 tttaggatat tttcctcaat ggattctctt ggcgtacctt ctgcttgagg atattgaata
    15961 agtgatttta tctccacatc gggcttcttt atagttttaa attccacgaa tccaggaaat
    16021 tgttcaagat acttattagt aatttctgga ggattctgtt tcaggacatt tagtccaata
    16081 tcggtaattt taaaataaga ctttctaggg gattctaaaa gtcccgcctt tttgagatat
    16141 gtccttgccc aacccgttct attattaatt ataggttgtt gcccactcga caaaagttct
    16201 cttttttctt cgttacttaa attaaataaa ccggaaatat gttcaattgc ttcccgtatt
    16261 gaatgttctt ttttatctcc agcaaacttc agtagcggaa gcattatact ttgataatcg
    16321 gggattgcca tatagaactt catttattct tacaagataa aaggattatt attttcatgg
    16381 caatatcaaa tcataaaaaa gtccgtatat tacaatactt tattgagatt gttgttgagg
    16441 aaaagctttg gacaagataa aaaaattata tatgaaaacc tatttaaccg cgttaatcat
    16501 tattatgata attatagtta tgagaaatga cccttgaaag aggggagata acccgaatga
    16561 ataaattact tcgtgtatta attgttgagg attccaaatt tgacgcccgg gtattgatcg
    16621 gagaacttga gaatggcggt tacaggctgg atcatgaact tgtggataat ccggaagata
    16681 tgagagcagc gcttggaaaa aagccatggg atatcgttat ctccgatttt gtcatgccga
    16741 atttttcagg aatggatgcg ataaggatct tgcaggaaag cggtcttgac atacccataa
    16801 taatcgtatc cggaaagatc ggggaggata ctgctgtgga atctatgaaa gccggagcgc
    16861 atgactatat cataaaaggc aatctggcaa ggcttatacc tgctatcgag agggaacttt
    16921 tcgaagcgga agagcgcaga aaaagaaagc gtgtagaagc tgaactaaaa agtaccaaag
    16981 aaaaactgga atcatttatt aacaacactt ctgatgctat cataatcatt gatatggaaa
    17041 aaaaaatcct gcaggtcaac aagggttttg aaaaattata tggctggact gccaccgaag
    17101 ctattggaat tcaattgcct ataataccgg ataatttaat gccggaaacc aaaaaactac
    17161 tggcagaggt agaatccgga aaagtcatta ctgctcatga aactataagg aggcggaaag
    17221 ataaaactct ttttgatgtc agtgtgacaa tttcaccaat aaaggatgca agtggaaaag
    17281 tagtcgcctt tgctggaatt tcaagggata taactgagag aaagagagcg gaagaacaaa
    17341 caatgtgttc tttattggag aaagaaaccc ttcttcggga aatccatcac agggtcaaaa
    17401 ataatatgca ggtcatatcc agtttgctca gacttcaatc aaaatatatc aaagacaagg
    17461 atgatttaga gattttcaag gatagtcaga acaggatctc ttcaatgtct cttgttcatg
    17521 agaaacttta tcagtccaat gactttacaa aaatcgattt tagtgtatat gtcaatgacc
    17581 tggtgaaaag cctgtttcac tcttatgggg ctatttcgaa caatattata ccaaatataa
    17641 aagtaaacaa tgtttcactt ggtattgatt cggcaatacc ttgcggtttg atcatcaatg
    17701 agcttgttac aaattcttta aaacatgcat ttcctgatgg cagaaaaggt gagataaggg
    17761 tttttatggg gcagacaggt gaaaaggaat ttgaattagt aatacgcgat aatggtgtgg
    17821 gcattgcgca gggaatcgat tttgacaaaa gcgacaccct aggcttgcat ctcgttagaa
    17881 tattagctga gaaccaactt catggagaaa taaaccttat tagaaatcac ggttctgaat
    17941 tcaaaattaa ttttaaagga gctaaataat gtccaaaaag caaatactga tcgttgaaga
    18001 tgaaagtata gtcgctgagg acctgagaca aagtttaatc ggtttaggat atgcggttcc
    18061 atctgttgaa tcttccgggg aaaaagctat tgaaaaaatt atggaaaagc gccctgacct
    18121 ggtacttatg gatattgtgt tgcatggtga aatggacggt attgacgccg caaaagaaat
    18181 acatgacagg ttcgatattc cggtcgtata tcttacttca tattccgatg aaaaaattct
    18241 tgatagagca aaaataaccg aaccatattg ttatatcatt aaacccttca aggagaggga
    18301 gcttcatatc aatatcgaga tagccaccta taaacataag atggaaaaga aattaaaaga
    18361 aagtaaacaa tggctggatt caacgttaaa aagccttgga gaagcagtta tttcaacaga
    18421 taaggaaggc ttcataaaga ccatgaatcc aattgcagag gcgatgacgg gttggaaaag
    18481 cgatgaagcg gcatcaaaac cgatttcgac agtttttaat ataatcagtg aaaaacccaa
    18541 tgtaaatgtg gaaaatccgg taaccaaggc aatccgggta ggaatgttct atgggcttgc
    18601 agagcatacg gtactggtta caaaagaagg attgaaagta cctgttgaca ttattggttc
    18661 tgtgatcaaa gatgatctgg gaaatatcat gggtatagta atgatctttg aggacattat
    18721 tgagcgtaaa cgcatggaag agagactcaa caaagcaaat tgcttatcgg tcaataatga
    18781 ccaaaagtga ccgatagact tttttagtcc attgttacct gttcgatcca gtgtatcttc
    18841 cctggcaaga agtcttcagg agttttgcca agttttaagg cttcaattac tgaaataacg
    18901 gattttacaa cagctataat attcatattc gtagtattga tctcaaaaac cgatttattt
    18961 ctctgtgtgg cttccactaa tattacatcc agcgcctcag catcagcatt ttcctttact
    19021 ttttggaatg agtaattctt cctttttccc aggcgctttc gcagggctac aggatgtgcc
    19081 cgcagaacga ttacggcatc tgctggcaaa agatggcaaa gatgcccttc cattattaca
    19141 tcccccgact tcttttcaat ttcatgtttc aagcgttctt ccagcttatc aatatcaact
    19201 tcataagtgt cccgcacttc gtcttttcct gtgtacagct tttcatcgag aacaagttta
    19261 ttcaaatcaa taatgctgta cagcttcctg tattttgagt gctcttttaa aacttcgcaa
    19321 actgatgttt ttcccgtacc cggcgtacct gttagagcga tcaacataat atttcacgaa
    19381 atgcttcgat gactttttcg ttctgttcct gcattccaac agatatccgt atcagcgact
    19441 tacccgcgcc cctgaaggat gtgcaatccc ggactattat cccttttttc aatagcaatt
    19501 cactgacctc tttagaactg ttcggggata catctacaag gataaaattc gcatctgaag
    19561 aatatacttt acataaagtc ccaactcctt ttgaaagctg catgcgccct tttttcacag
    19621 cttcaacact cttattcaga tgcttcttat cgccaagagc tgctaatccc gcagccgttg
    19681 acaatatatc cattgaaaat ggagtcatca ctttcatata ttcccttacg atccattcag
    19741 gcgcgattgc ataacccatg cgcattccgg caaggccaaa ggcttttgag aaagtgcgtc
    19801 ctacaataag attttcgtat tccctgacaa gtccggtgat actgctgttg gcaaattcaa
    19861 cataagcttc atctatgaat actatggcat cggtagattc caggatcttt cgtgcatctt
    19921 tttcggagat tgaattaccc gttgggttgt tgggagagca gagaaatatc attctggtat
    19981 ttacattagc tttatccaga atattttccg gggcaatata ataatcagaa tcccgctcaa
    20041 cgaacacggg ttttcctcca ttggcaaggg tcgctatctc ataataggaa aatgtcggta
    20101 tcggaattat tgattccgtc ccttgcgaca taaagagttt cattatggta tcaattatcc
    20161 cgtccatacc attacccgat accacgatat tactggaagg ataaccagta tactctccaa
    20221 tggcttttct taatacaagc gcatccgcct gtggatacag gtgtactctt cctgcttttt
    20281 cttttatggc cgcgacggcc cggggtgatg gcccaagcgg attttcattt gaacccaatt
    20341 tgattatgga atcaggatca agtccgtatt ttcctgctat ctcctcgatg gatttcccgg
    20401 gaacatactc cttgatatcc ttaactgaag gccgaaccag ttctttaaat ctcattcagc
    20461 accgacacca ccgtagcaag ctgttgttct gttatagtaa gcggcgggac aaaacgaagc
    20521 actgattctg acgtacaatt aagaaggacc cctttttcac gggcgcctgc cacgatatct
    20581 tcacacttta tcgaaagttc catgcccacc atcagtcctt tgcccctgat ctccagaatg
    20641 taatccttat tgagctgtcg aagtttcgaa acaagataat ttcccatatc atatgatctt
    20701 tttacaagtt tttctttctt tataacatcg atatttgcaa gggccgccgc acaagccagg
    20761 gcactgccgc caaaagttga ggcatggtca ccgcgcacga aatttgccgc cacatcctca
    20821 ccagcaagca ttgcacccat cgggaagcct cctcccatcg cttttgccgt tgtcattata
    20881 tcggggatga taccggaatg ctctcttgcg aaccattttc cagtcctgcc aaatcctgtc
    20941 tgcacctcat caagtattaa caaaacgctc ttatcatcgc atatctccct tacttccttt
    21001 agataatcat cagatgggat tatcactccg ccttctccct gtatgggttc gattattaat
    21061 cctgcagtat tctcagtgat cgcagaccgg atggcctcag tatcattata cgggacaaaa
    21121 actgcgggag caagcggttc aaagggtttc ctgtattttt ctttatgtgt gacacttaaa
    21181 gcaccgcggg tgcgtccgtg aaatgcatgt tctgtcgcga tgaactcttt ttttcctgaa
    21241 gctttcctgg caagtttcag ggcgccttca actgcttcag ctccggaatt gcagtaaaat
    21301 acgcgctcca tgcctgaaat gctggcaagt tcttcggcaa gaagagcctg attttcggta
    21361 taatacaggt tcgaaacatg aattaactgc tc
//
LOCUS       DATGBN010000447         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_424216, whole genome shotgun sequence.
ACCESSION   DATGBN010000447 DATGBN010000000
VERSION     DATGBN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424216"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..379
                     /locus_tag="VKL21_11400"
     CDS             68..379
                     /locus_tag="VKL21_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMB46413.1"
                     /translation="MAFGPLEIVLIVVVIIVLFGASKIPELARSLGKATGEFKKGKQD
                     IDNELNEIVKPGKEIKPPENSSSKIIKMAQDLGIATEGKSDEQLLDEIQKKMIKIEKK
                     V"
     gene            complement(384..794)
                     /locus_tag="VKL21_11405"
     CDS             complement(384..794)
                     /locus_tag="VKL21_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1699 family protein"
                     /protein_id="HMB46414.1"
                     /translation="MRIRVVSSKEEIGSLGAGEKIIHLAFRPSNKDIFALVQTCPNVK
                     AIHVPSSYIKTISGSTRMFLEMQGISLLEGDVWGHRKDINEYSEVKPAVFERLEELKS
                     EGLSESAVLDRLGRETRLSKDLLKFLIKEKVKKK"
     gene            complement(948..>1516)
                     /locus_tag="VKL21_11410"
     CDS             complement(948..>1516)
                     /locus_tag="VKL21_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HMB46415.1"
                     /translation="TPYTSLSVAIIGTVKKGCEIKSSTAKAGDVVIAAYDLDGKVGPN
                     SPYSFDTTTAKEPEEVRGKYRVMQKIGEKKLVTAGKDISNPGLIGTLGMLLETSKKGA
                     HVDLGKISTPEKFDLTRWLKVHPATGFIVTALPENESEVIGLFEKAGYTAANIGVVDD
                     TRKLDICFNDKCVTVFDFKKDVVTGITQ"
BASE COUNT          450 a          322 c          267 g          477 t
ORIGIN      
        1 ttgaagaaat acgcttgaag atcagatcaa aagagacaaa aaccgttaca aatactgcag
       61 gataattatg gcatttggtc ctttggaaat agttttaatc gttgtggtta taatagtatt
      121 gtttggtgcg agtaagatcc ctgagctagc caggtcgctg ggtaaagcga caggtgaatt
      181 taaaaaagga aagcaagata tagataatga attaaatgaa atagttaaac ccggtaaaga
      241 aatcaagcct ccagaaaatt catcctcaaa gataataaaa atggcgcaag accttggaat
      301 tgcgaccgaa ggcaaaagcg atgagcaatt gcttgatgaa atacagaaaa aaatgataaa
      361 aatagaaaaa aaggtctaaa aatttatttc tttttaacct tttcttttat caggaatttt
      421 aaaagatctt tgctaaggcg tgtttctctt ccaagcctgt ccagtacagc agattcggac
      481 aaaccttcgg attttagctc ttccaacctt tcgaatactg caggtttgac ttccgaatat
      541 tcattgatat cttttctatg cccccataca tctccttcca gaagagaaat tccctgcatt
      601 tccaggaaca ttctggttga accggatatg gtcttaatgt acgaacttgg gacatggatc
      661 gctttgacgt tggggcaggt ttgcaccaat gcaaaaatat ctttattaga cggcctgaaa
      721 gcaagatgaa ttattttttc cccggccccc agtgacccaa tttcttcttt tgaacttaca
      781 actcgaatcc tcatattatt ttacctctgt gctatttgat atacaatcag tttctcacaa
      841 tgcatgaaaa tcgatccatc acacaacata tctaatatgc aatttcaccc tataaataat
      901 cttcgctatt taaatgatat ttagaataag ttatataaat cattggtcta ttgcgttatc
      961 cctgtgacca catctttctt gaaatcaaat actgttacgc atttatcatt gaaacaaata
     1021 tcaagcttcc gcgtatcatc cactacccca atatttgcgg ctgtatatcc tgctttttca
     1081 aacaggccaa tgacttcgga ttcattttca ggtaaagccg tgactataaa acctgtcgca
     1141 ggatgaacct tcaaccaccg ggtaagatca aatttctcag gcgtcgatat ttttccaagg
     1201 tcaacgtgcg cccctttttt acttgtttca agaagcatcc caagtgtccc gataagccct
     1261 gggttgctga tatcttttcc tgcagttaca agtttcttct cgcctatttt ctgcattacc
     1321 ctgtatttcc cgcgcacttc ttccggctct tttgcggtgg ttgtatcaaa actgtaaggt
     1381 gagttcggcc ctactttacc atccagatcg tatgccgcaa ttacaacatc tcctgcctta
     1441 gctgtgctac ttttgatctc gcatcccttt ttaactgtgc ctatgatcgc gacggataat
     1501 gatgtatatg gagtat
//
LOCUS       DATGBN010000448         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_230018, whole genome shotgun sequence.
ACCESSION   DATGBN010000448 DATGBN010000000
VERSION     DATGBN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230018"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VKL21_11415"
     CDS             <1..309
                     /locus_tag="VKL21_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46416.1"
                     /translation="VRRRVPCQVDSSEKEINSLVWVENKTDCEKGVYLKFFKIVSWQT
                     RTLHCYAKTVKFVSNLIKHDSRILAISTEGDTDPVNFRRITWDGIFQNIKRGIKHAYR
                     "
     gene            296..1480
                     /locus_tag="VKL21_11420"
     CDS             296..1480
                     /locus_tag="VKL21_11420"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HMB46417.1"
                     /translation="MLTDERKQKVISLLQDLIRLKSYSGEENDVVERLKEAFTAMGYD
                     DVFVDVYGNIIGHIKGKKPGKKILFDGHMDTVPVPDTSKWSQNPFGADIVDGKIYGRG
                     ASDMKGALCAMINAGSFFAEDMNKDFAGDIYVAGVVHEECFEGVAARKISERVKPDYV
                     VIGEASELNLKIGQRGRAEIVVETYGKPAHSANPQKGVNAVYKMAKLIERIRNIDMTH
                     DDFLGNGILELTDIKSSPYPGASVVPDYCRATYDRRLLVGETKESVLKPIYDIISKME
                     KEDPEFKAKAYYAKGEEKCYTGSKIGDERFFPGWKYDRNEEFIQNVYSELKNAGLNPE
                     ITHYSFCTNGSHYAGEKGIKTLGFGPSPENLAHTIDEYVEVEQLLKATEGYTSIIKAL
                     LK"
BASE COUNT          637 a          249 c          393 g          495 t
ORIGIN      
        1 gtccgacgta gggtcccatg tcaagttgac tccagcgaaa aggagataaa ttctcttgtt
       61 tgggtagaaa acaagaccga ttgtgagaaa ggagtttatc ttaagttttt caaaatcgtg
      121 tcttggcaaa cgaggacatt acactgttat gcgaaaacag tcaaatttgt atcaaatctt
      181 ataaagcatg attcaaggat tcttgcaata agtacagagg gagacacaga tcctgttaat
      241 tttagaagaa ttacttggga tggaatattc caaaatataa aaagggggat taaacatgct
      301 tacagatgaa agaaaacaaa aggttatctc acttcttcag gatttaataa gattaaaaag
      361 ttactctggt gaagaaaatg acgttgttga aagattgaaa gaagccttta ccgcaatggg
      421 atatgatgat gtttttgtag atgtatatgg aaatataata ggacatataa aggggaaaaa
      481 gccaggaaag aaaatattgt ttgatggaca tatggatact gttccggtgc cggatacatc
      541 taaatggagt caaaatcctt ttggagcaga catagttgat gggaaaatat atggcagggg
      601 agcatcagat atgaaaggtg cactttgtgc aatgattaat gccggatcat tctttgctga
      661 agatatgaat aaagattttg caggagatat ttatgtcgca ggtgttgttc atgaagaatg
      721 ctttgaaggt gttgcagcaa gaaaaatcag tgaaagggta aagcctgatt atgttgtaat
      781 tggtgaagcc tctgaactaa atttaaaaat tggacaaaga ggaagagctg agatagttgt
      841 tgaaacctat ggaaaaccag ctcattcagc aaatcctcaa aagggagtca atgcagttta
      901 caaaatggcg aagcttattg aaagaatcag aaatattgat atgactcatg atgatttttt
      961 agggaatgga atacttgaat taacagatat taaatcatca ccatacccag gagcatcagt
     1021 tgtgccagat tactgtagag caacttatga caggaggctt cttgttggtg aaacaaaaga
     1081 gagtgtatta aaaccaattt atgacattat ttcaaaaatg gagaaagaag accctgaatt
     1141 taaagcaaag gcatattatg ctaagggtga agaaaaatgt tatacgggaa gtaaaattgg
     1201 agatgagaga ttttttccag gatggaagta tgacagaaat gaagaattca ttcaaaatgt
     1261 ttacagcgaa cttaagaatg cagggcttaa tcctgagata acacattatt cattttgcac
     1321 aaatggaagc cactatgcag gggaaaaggg aataaaaaca ctaggatttg gaccttcacc
     1381 tgaaaatctt gcacatacaa ttgatgaata tgtcgaagta gaacaattgt taaaggctac
     1441 agagggatac acatcaatta tcaaggcgct acttaaataa tattcaaaaa gggatgtaga
     1501 tatgagcatt ttttttgttt cacttggtag tgcgttatgg ggccctatca ttttcactgg
     1561 cggtttgtta aactaagtgc aacaattctt ctaccaaagt ctgataagac attattgttg
     1621 cagtctaaca aaccgctaat caaaaaatgt tatacaagga gacaaaacga aaacatcaag
     1681 tttagtccgc tgaagaggtt gtaattttta gagagtcagc cgccaaggta agtctccttt
     1741 acctgcgtgt tttctactaa ctcggggcca gttc
//
LOCUS       DATGBN010000449         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_113882, whole genome shotgun sequence.
ACCESSION   DATGBN010000449 DATGBN010000000
VERSION     DATGBN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113882"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VKL21_11425"
     CDS             <1..476
                     /locus_tag="VKL21_11425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB46418.1"
                     /translation="GLDMISILYFHSREIKRQILEFKPDVIIAFGILNAYIGLHYAKK
                     NSIPFVYYLLDHLHRLLPNRKLQKIAKQFEKMNIMNADRILVLNKGLKDYAIDMGGNI
                     NKVSIVTSGIDNDKFNPQVNGSSIRERYGIKKDDVLLFFMGWIYDFSGMKGRPLI"
     gene            complement(653..1072)
                     /locus_tag="VKL21_11430"
     CDS             complement(653..1072)
                     /locus_tag="VKL21_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HMB46419.1"
                     /translation="MGEKTVIVLHSGDMDKVYSALIIGNGALAMGMEASIYFTFWGLM
                     RLKKGELEKGPLSKMNMLGLGRLMVKKRMNKANVVSLERLMSDFKELGGKIIACEMTM
                     EIMGIKKEELRTEWIDEWGAVGTYIQEARDANITLFI"
     gene            complement(1092..1319)
                     /locus_tag="VKL21_11435"
     CDS             complement(1092..1319)
                     /locus_tag="VKL21_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HMB46420.1"
                     /translation="MVEMEIDARGQTCPVPLVECRKAFKRASPGDIIIVKGTHPASKK
                     EIPMACKAMGLEVLEIKDKDEGKEWEIKIRR"
     gene            complement(1567..1917)
                     /locus_tag="VKL21_11440"
     CDS             complement(1567..1917)
                     /locus_tag="VKL21_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HMB46421.1"
                     /translation="MNRSECNLSLDELLKFDGVMAAGIFSPEGKLVDYKSKTGMPKEM
                     TQMTAKFCGAVNMMFDVLASAYTQLYKMNWVPQHNWMYSGGDWTVIISGTRGVFVESS
                     NADLEKLLKALGMC"
BASE COUNT          690 a          530 c          418 g          794 t
ORIGIN      
        1 ctggtttaga tatgatttct atcctatatt tccattctag agaaataaaa agacagatat
       61 tagaatttaa acccgatgta ataattgcat ttggaattct taatgcatac attggattgc
      121 attatgctaa aaagaatagc attccatttg tgtactatct tttggaccat cttcataggc
      181 ttctcccaaa ccggaaactt caaaaaatag ccaaacaatt cgaaaaaatg aatattatga
      241 atgctgatcg gattttagtc cttaataagg gattaaaaga ctatgcaata gatatggggg
      301 gaaacataaa taaggtttca attgttactt ctggaatcga taatgataag tttaatccgc
      361 aggtaaatgg ttcttctatc agagaaagat atgggattaa gaaagatgat gttctactat
      421 tcttcatggg atggatatat gacttctcag gaatgaaggg tcgtccactg atatagggtt
      481 ggtgttttag ggggaaatgt ctaaagctcc tcttgattgc gttttatgtt attgcgacac
      541 gatagctcta aaaactccaa agtcacgaga aaaggacgag ggtagacgtt attttgatta
      601 ttgtggaata acaacaactg aatacaaatg gactaccaaa gctcttcaag actcagataa
      661 agagagttat gttcgcatcc cttgcttcct ggatataagt acctacagct ccccattcgt
      721 cgatccattc ggttctaagt tcttctttct ttatgcccat gatttccatg gtcatttcgc
      781 aggctattat ctttcctcca agttccttga agtcactcat tagtctttca agtgaaacaa
      841 cgttggcctt attcatcctc tttttcacca tcagcctgcc aagtcccagc atattcatct
      901 tggaaagcgg ccctttttca agctctcctt ttttcaaccg cattagtccc cagaaggtga
      961 aatatatgga ggcttccatg cccattgcca gggctccgtt tccaattata agcgcactat
     1021 atactttgtc catatcacca ctgtgcagga caatgactgt cttttccccc atttccattc
     1081 ctcttccggc tttatctccg gatctttatc tcccactctt ttccttcgtc tttatcttta
     1141 atttccagca cctcgagccc cattgctttg caggccatag gaatctcttt cttagatgcc
     1201 ggatgcgtgc ccttcacaat tataatgtcc cctggcgatg ctctcttgaa tgcttttctg
     1261 cattctacca gcggaaccgg gcatgtctgc cccctagcat cgatttccat ttctaccact
     1321 atttttatcc cctagattcg agaaacggaa ggatcctgac aacatttctg ctgcttttct
     1381 ctaaattcct gtattttaaa aatatacatc gcttgatgtt gaaaatacta tttaggaatc
     1441 accatggtct tcttattcgg atctgtcgtt cgcttcagag tgctctcctg attttcttaa
     1501 gggcaaatct ctctaaaaat gattagctaa ccagggactg gctagccagg tttatttaag
     1561 tggatgttaa cacattccca gggccttaag aagcttctcc aaatctgcat tggagctctc
     1621 gacgaaaacg ccccttgtac ccgagattat aacggtccaa tcgccgccac tgtacatcca
     1681 gttatgctgg ggtacccagt tcattttata gagttgtgtg taggcactgg ccagaacatc
     1741 gaacatcata ttcacagctc cgcagaactt ggcggtcatc tgggtcatct cttttggcat
     1801 cccggttttg gatttgtaat ccacaagctt tccttctgga ctgaaaatcc cagctgccat
     1861 tacgccatcg aatttcaaaa gctcatctaa cgatagattg cactcacttc tgttcataca
     1921 ctaattccct caacttttta ttattttctg gaatctaaaa atttagcatt aattcctagc
     1981 aattgaccat cataaataaa aaacccagaa aacaataaaa agtatcttat ttctcttaaa
     2041 tataacttta ggtggttctg ttgcaaaaat aattttactg cctttttagg ctataactat
     2101 tgctttttgt atcattattg gcccgtttat ggcatataaa tatcccattt gtgatgtttt
     2161 tcactggttt tgtaaaatag ccagattttt tgcaacaaaa ccgtatttta tcatttttca
     2221 gttcatttat agcatatcag tacttcacca actggatccg aaaatcatct atccccatta
     2281 cgatttcaaa catttaaccg aaaagtattt tgaaatcgga atgaagctaa atgatttctc
     2341 gatccagttg gtgaagtact gccatagaat ataaatacat cccagaaatg aaggcagaag
     2401 cccgggaact gggaaaagaa aaacttccag ga
//
LOCUS       DATGBN010000450         5572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_35395, whole genome shotgun sequence.
ACCESSION   DATGBN010000450 DATGBN010000000
VERSION     DATGBN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35395"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1375)
                     /locus_tag="VKL21_11445"
     CDS             complement(170..1375)
                     /locus_tag="VKL21_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HMB46422.1"
                     /translation="MDTNKQYTWWLKALSGADEVQARRFAGAIALEMGWGGISKIEEL
                     TGMSHSTISKGIEELKTSDKLEQPERLRASGGGRKKVEIKDPGIIADLETIMDENTSG
                     DPMSLLKWSNKSTYKIAEELVKLKHKIDPDTVGRLLKENDYSLQANKKNIEGSSVPER
                     DAQFRYINERAKEFINQEYPVISVDAKKKENVGEFKNPGQNWRKKGKAREVNVYDFAS
                     LGIGRATPYGIYDPKRNEGMVNVGMSYDTSEFAVESIRQWWLLFGRNNYSNAKGLMIC
                     SDGGGSNGSRNKGWKIHLQELVDQIKIPITVCHYPPGTSKWNKIEHCMFSFISKNWKG
                     TPLVSFEAIIKLIGATKTKKGLTVAARLDEKEYLKGIKYANEDMAKLHLESHDLHPKW
                     NYIILPKSD"
     gene            1946..2095
                     /locus_tag="VKL21_11450"
     CDS             1946..2095
                     /locus_tag="VKL21_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46423.1"
                     /translation="MKDEVARMKNEFKSNVDELREMYQSIMHDEIENMVSDNFAEARK
                     KKLKA"
     gene            2437..2772
                     /locus_tag="VKL21_11455"
     CDS             2437..2772
                     /locus_tag="VKL21_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMB46424.1"
                     /translation="MQWSDYMTEHPLKIYENLDPDLLKHVENSRDFVFADGALPKKFK
                     LLIAMAFDASYGAVNGVKSLAQQAIEAGATKEEITEALRVAQYISGVGCVYTAAQGLK
                     DLLESPNRE"
     gene            2868..3269
                     /locus_tag="VKL21_11460"
     CDS             2868..3269
                     /locus_tag="VKL21_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMB46425.1"
                     /translation="MIKTIDHIGIMTNDLQQSVEFYTDVLGFSVSAKIEMEEIGFSAI
                     FVEKNGGKIELIGYRGEIPKRSEGIEIKMGGGSIPINDHITFTVDDIEATVTELKEKG
                     VEFVLEPIQVEGGMKIASFKDPNGVLIELVE"
     gene            3407..3646
                     /locus_tag="VKL21_11465"
     CDS             3407..3646
                     /locus_tag="VKL21_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46426.1"
                     /translation="MQKEEEKTMLKILTKKIDELNKTINEEKIYSEEKRKENPIAYVA
                     GAFVGGLVAGFLIGNEKNKERRRNRFRSRVRWSLD"
     gene            complement(3914..4834)
                     /locus_tag="VKL21_11470"
     CDS             complement(3914..4834)
                     /locus_tag="VKL21_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46427.1"
                     /translation="MSDENILHCDRCEPEYPEKKGKILLGNINPGTDCTIAFYLDPLI
                     CAKEGTDINCRIFFKDAYGRSDIVQMEPLKIKVICPIFRTEQDNIGRLKELIEELRFC
                     DSKVYTFPKRLELQELLRSCRDVIQLHDVRHIKTFKTTDEKTYESWYYGKTKVTKKDL
                     VIKCAIRKDAESIEVLASGNDPSDITGLLAELGRNLTKEFEKIGKVQPVFNITIKDSI
                     IQRSNLLSFCDLDGSCGGNVVIEDSLVQRSNIGSWNETQQKNEEEERKRKDERLREES
                     EEQVKLDLKEERETNEKLQVQYWIATSNWL"
     gene            complement(4824..4976)
                     /locus_tag="VKL21_11475"
     CDS             complement(4824..4976)
                     /locus_tag="VKL21_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46428.1"
                     /translation="MDTLDTSHEIAIKPILLREFEFFGGCIRVKISVKNKSNLAILDT
                     ALELER"
     gene            complement(5121..5429)
                     /locus_tag="VKL21_11480"
     CDS             complement(5121..5429)
                     /locus_tag="VKL21_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46429.1"
                     /translation="MNAEIFKDFYQTEKIPKLKHEKFVPFEMGELTLKGNITINFGKY
                     SGNSGIHCRNAQGIEVVPPGKHLGNSISEMATKLKDFVNSPQFRNAKTDAEAEKYWGS
                     "
BASE COUNT         1676 a         1093 c         1003 g         1800 t
ORIGIN      
        1 tcggacatat tgaaatgatg taaattatgg gttagctcca atgtggttaa aatccacagt
       61 cagcccatga tttacctcct ataaaaaaat gaaaggtgaa atatggaaaa tatagaataa
      121 acatctttag aacctgtctt aggaattacc taaaaataac ttatctaaat tagtcacttt
      181 taggtaaaat aatataattc cattttggat gtaaatcatg agattccaaa tgcaattttg
      241 ccatatcttc atttgcatat tttattcctt taagatattc tttctcatct aaacgtgctg
      301 ctacagtaag accttttttt gttttcgtgg cacctataag tttaataatt gcttcaaaac
      361 tgacaagtgg cgttcccttc caattctttg aaataaatga aaacatgcaa tgttcaatct
      421 tattccactt gcttgttccg gggggataat ggcagaccgt tatgggtatc ttaatctgat
      481 caactaattc ctgtaaatga attttccagc ccttattgcg acttccatta cttcccccac
      541 catctgaaca tatcattaat ccttttgcat tcgaatagtt gtttcttcca aaaagaagcc
      601 accattgcct gatgctttca acagcaaatt cagatgtgtc atagctcatc cccacattta
      661 ccattccttc atttctcttg ggatcataaa tcccatatgg tgtcgctctt ccaatcccaa
      721 gtgaggcgaa atcatacaca tttacttctc tggctttgcc tttttttctc caattttgac
      781 cggggttttt aaattccccc acattttcct tcttttttgc atcaaccgat attactggat
      841 actcttgatt tataaattct tttgctcgtt catttatata cctaaactgg gcgtctcttt
      901 ctggtactga agaaccttcg atatttttct tgtttgcttg aagtgagtaa tcattttctt
      961 ttaaaagacg acctactgta tctggatcga ttttatgttt tagttttact aattcttcag
     1021 cgattttgta ggtggattta ttcgaccatt ttagaagact cattggatct cctgaagtgt
     1081 tttcatccat gatcgtttca agatccgcaa tgattccagg atcttttatt tcgacctttt
     1141 ttcttcctcc cccagatgct cgaagtcttt ctggttgttc aagtttatct gaagttttga
     1201 gttcttctat tcctttactg atggttgaat gagacattcc ggttaactct tctatttttg
     1261 agattccacc ccaacccatt tcaagtgcta tagctcctgc aaaacgcctt gcttgaactt
     1321 catcagctcc agatagggct tttagccacc aagtatactg cttgtttgta tccatgccac
     1381 catatatgat ggcttacata ataaactttg ctaagttatt tttcggtatt gccttagtta
     1441 tattatcatg cccaatattc aatacttttg tgttaccaga atttccaaaa agggctttta
     1501 tctggatcac cctctgggaa gactatcctt attgtccgat cgcactcata cgtatcctga
     1561 gagattttta agcccgtaga tggcacattt taggtcagca atggaataac attttaaaca
     1621 gaaatactct gctctgacag tatatttttt atttttaaac gtatccctac aatccttcct
     1681 atcattactc ctgcgagaat actataagtg aaggcggtga ggacagaacc acgtatctaa
     1741 tgcccaaaaa gccatgttct tgatgcggaa aaagccatat ataacaccat tacaaaaatg
     1801 ccaatcgtat caaaacgaga aacaactttt gccctctctt cattccagtg aacaataaac
     1861 atgcgagtgg caatgaatcc aaggaataat cccacgagaa atccggcaag ggatagctcg
     1921 atgcctatga aaacttaacg ctgatttgaa agatgaagtt gcaaggatga agaatgaatt
     1981 taaatctaat gttgatgagt tacgtgagat gtatcaatca ataatgcatg atgaaataga
     2041 aaatatggta tccgataact ttgctgaagc aagaaagaaa aaacttaaag cctaaaccct
     2101 tgtgagctat ataaggaatt gaactcgaca cgcaaataaa tatgtcgttt ctcacaaggg
     2161 gcagacttct ttattgtgct ttcactatgg ttagaataaa agcacaactt ctaatatcta
     2221 caagtgaata taaaaagata tgtttaccct aacatgaata taattataac cattatagat
     2281 aaaataatga ttttttcgaa ccgaccctat catctattgg gcttttacag tatttccgct
     2341 atctacaaaa gatgaagtcc agaaatccaa atatgataaa ccgttatact ggagtttttc
     2401 aataatctta atcaccatta gccatcattg tagtatatgc aatggagtga ttatatgaca
     2461 gaacatccct taaaaatcta tgagaactta gaccctgatc tacttaaaca tgttgaaaat
     2521 tcaagagact ttgtctttgc tgatggcgct ttacccaaaa aattcaagtt gcttattgca
     2581 atggctttcg atgcatccta cggagcggtt aatggcgtaa agtcgctggc tcaacaggca
     2641 atcgaagctg gcgccacgaa agaagaaatc acagaagcat tgagagtcgc acaatatatc
     2701 agtggagttg gttgcgttta taccgcagca caaggactaa aggatttatt agaaagcccc
     2761 aacagagaat aaaccgtata agtcatacca tgaaaggtat agacgctgtt tgcagataat
     2821 ggaaaaaagt gaatgcttga atttgtagaa aatgggagaa aaaaaccatg attaaaacaa
     2881 ttgaccatat cgggattatg acgaatgact tacagcagtc agtggaattc tatactgatg
     2941 tgcttggctt ttctgtttcc gctaagattg aaatggagga gataggtttt tctgcaattt
     3001 ttgtagaaaa aaacggcggc aagatagaac tcataggata tagaggcgaa atcccaaagc
     3061 gttcagaggg tattgaaatt aaaatgggtg ggggttcaat tcccattaac gaccacatta
     3121 cttttaccgt agatgacatc gaagccacgg taaccgagct taaagaaaaa ggagtcgagt
     3181 ttgtcttgga gccaatacaa gtagaagggg gaatgaaaat tgcttccttc aaagacccga
     3241 acggtgtgct gattgaactg gtggaataga ccgcatgaat gagaactatt gcctttggac
     3301 tccaccagct aaaactggtc gctagttgga gacttgttgg ctgcccaagt tgggaaacta
     3361 gcttggtggg aatgaaataa aatatatcat aaaaggagat taaattatgc aaaaagaaga
     3421 agaaaagact atgttgaaaa tattaaccaa aaaaattgac gaattaaata aaacaattaa
     3481 tgaggaaaag atatactcag aagagaagag gaaggaaaat cctattgctt atgtagcggg
     3541 ggcttttgtt ggaggtcttg tcgcaggttt cttgattgga aatgaaaaaa ataaggagag
     3601 aagacgtaat cgatttcgtt cacgggttcg atggtcattg gattagaata ataaaaaatt
     3661 gaatgttgta tacaataacg aagaagacaa aaaaatcaaa tatacaaaaa cataaaaggg
     3721 aaatgagttg caggttttaa gccaaccgtc atatgattct gtccagcaaa tatctgcttc
     3781 cacatctctc gcaggttggt agattatagc tttatcattg ggcctgactc tatccctata
     3841 tttatgatac caccatattt gtttttcttg acgatcgaac gctccagagc aagggcgaga
     3901 tgatctgcgt tgatcataac caatttgatg ttgctatcca atattgaacc tgtaattttt
     3961 catttgtttc tctttcttct ttcaaatcaa gttttacttg ttcttcactt tcttccctga
     4021 ggcgctcatc tttacgcttt ctctcctctt cttcattctt ttgctgagtc tcattccacg
     4081 aaccaatatt cgacctctgc accagactat cctcgatcac cacattcccg ccgcagcttc
     4141 catcaagatc gcagaagctc agaagattgc tcctctgtat aatggaatcc ttgatagtga
     4201 tattgaaaac aggctggact tttccgattt tctcaaattc cttcgtgaga ttgcgcccca
     4261 gctcagcaag aagccctgtg atatcgctgg ggtcattccc tgatgcaaga acctcgatac
     4321 tctctgcatc ttttcttatg gcgcatttta tcacaaggtc tttcttggta acctttgtct
     4381 tcccgtaata ccatgattcg taagtttttt catccgtggt tttgaaggtc tttatatgcc
     4441 gcacatcatg aagctggatt acatccctgc atgatctcaa aagctcctgc aattccagcc
     4501 ttttcgggaa agtatagacc ttgctgtcgc agaatcgaag ttcttcaata agttccttca
     4561 gccttcctat attgtcctgc tcagtacgga atattgggca tatcactttt atcttcagtg
     4621 gctccatctg aacaatgtca gacctgccat aagcatcctt aaaaaaaatg cggcagttga
     4681 tatccgtacc ttcttttgcg catatcaaag ggtcaagata gaaggcgatc gtgcagtctg
     4741 tgcctggatt gatgtttccg agcagtatct tgcctttctt ctctggatat tcaggttcgc
     4801 aacggtcaca atgaagtata ttctcatcgc tcaagttcaa gtgctgtatc aaggattgca
     4861 aggttggatt tgttctttac agagatcttt acacggatac atccgccgaa aaactcgaac
     4921 tctctgagta gaatgggttt gattgcgatt tcgtgagatg tgtccagtgt atccatactt
     4981 taattatctt tatataatat tctctgcata tagttcgtct gtacccaatc ttattaacat
     5041 atatttctgc ttcctcaatg tgaaatatta cttgataatc tcatgatttg atacgctaaa
     5101 aaatacttgg tgaaattcta ttacgaaccc cagtattttt cggcttccgc gtccgttttt
     5161 gcatttctga attgtgggga atttacgaag tcttttaact tagttgccat ttcggaaatg
     5221 ctattaccca ggtgcttacc cggcggtaca acttctatac cctgtgcgtt tctacagtgt
     5281 ataccggaat tcccggaata cttaccaaag tttatggtaa tattcccttt cagggtaagt
     5341 tcacccattt caaagggtac aaatttttca tgcttaagtt ttggaatttt ttcagtttgg
     5401 tagaaatcct taaaaatttc agcattcaag tacaccaatg gcttagcagt aatgctaaaa
     5461 gctattatga taccatgtca ttcgatgaat gattggtaaa tttttaatag ttctttagca
     5521 tgcatgtgtt tttaataaaa ccaatgctaa aattcctaat caatatgcat cc
//
LOCUS       DATGBN010000451         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_501782, whole genome shotgun sequence.
ACCESSION   DATGBN010000451 DATGBN010000000
VERSION     DATGBN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501782"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VKL21_11485"
     CDS             complement(<1..853)
                     /locus_tag="VKL21_11485"
                     /inference="COORDINATES: protein motif:HMM:NF001624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HMB46430.1"
                     /translation="MKEFLSWDQTLFKDSELFELDHIPEYFHHRDAQMQSLMHCVRPA
                     LGGGRPVNSLCIGSPGTGKTTAVLKVFEEIEKHSQNVVPVLVNCQVNSTKYAVFSQIF
                     KKIIGYAPPATGASFKKIFSEVAKHIVEHEKVLLVALDDMNYLFYENEVDGILYSLLR
                     AHETHPGARMGVIAILSDTGVPHLLDPKVESVFLPEEIKFPQYSFDEIRDILLNRIRL
                     GFFPDVLGESVLENIAQHTFSLGDLRVGIDLLKRSGLNAEKRASKTIAQEDVESAYEK
                     SRLVHLSY"
     gene            1086..2198
                     /gene="pstS"
                     /locus_tag="VKL21_11490"
     CDS             1086..2198
                     /gene="pstS"
                     /locus_tag="VKL21_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HMB46431.1"
                     /translation="MKRKIIVIILILAGVLLAGCVSKPAEVQPTTVQPTKAPETITLI
                     GAGATFPYPLLSKWSSEYNKINPNIQINYQSVGSGAGIQQITARTVNFGATDAPLTEQ
                     EFSSVSGILQIPETTGAVVVAYNLPGIQKGVKLSGDVVADIFLGKINKWNDPRITLIN
                     PDIQFPDKEIIVVHRSDGSGTTFVFTDYLSAVSPDWKSKVGKGKSVNWPVGLGGKGNE
                     GVSGLLSKNPKSQNNYTIGYVELAFAKLQNIPYAYIKNKAGKFIEPTLETTASAAAGA
                     VPNLPAGDASWSSVSIVDAPGDNSYPIGSFTYLLVYKDQTDNTNGKALAEFLWWAVHD
                     GQKYSSDLLYVPLPEQVISLNEKTIRLMNYNGQPII"
     gene            2199..>2443
                     /locus_tag="VKL21_11495"
     CDS             2199..>2443
                     /locus_tag="VKL21_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46432.1"
                     /translation="MDGTKLSLIRFSRKKLSGDFIFEAIVGLFALSILILAFFLFREL
                     FIGSGLSRNTFGLSFLVTSTWNPVNEIFGALPFIFGTL"
BASE COUNT          709 a          529 c          512 g          693 t
ORIGIN      
        1 tgtaagaaag atgcacaagt cgcgatttct catacgctga ttccacatct tcctgcgcta
       61 tggtcttgct tgcccgcttc tctgcgttaa gtcctgagcg ctttaataaa tctattccca
      121 cgcgcagatc accgagtgaa aaagtgtgct gcgctatgtt ctcaaggacg ctttccccaa
      181 gcacatccgg aaaaaatccc agccttatgc gatttaacag gatgtcgcgt atctcgtcga
      241 atgaatactg tgggaatttt atctcttcag gcaagaacac cgattcgact ttagggtcaa
      301 gtaaatgggg tacgcccgtg tcgcttaata ttgcgattac gcccattcta gctcccggat
      361 gcgtttcatg ggctcttaga agcgaataca atatcccatc cacttcattt tcatagaaca
      421 ggtaattcat atcatcaagc gctacaagaa gcactttctc atgctctact atgtgttttg
      481 caacttcgct gaatattttc ttaaaggaag ctcccgttgc tggcggagca taaccgataa
      541 ttttcttgaa tatctgtgaa aaaacagcat attttgttga attgacctgg caattgacaa
      601 gcacaggcac tacattttgc gagtgttttt cgatctcctc aaaaacctta agcactgccg
      661 tagtcttgcc agttccaggg gagcctatgc aaagactgtt cactggcctt cctccaccca
      721 atgcggggcg aacgcaatgc atgagcgatt gcatttgtgc gtccctgtga tggaaatatt
      781 cagggatatg gtcaagttcg aataactcgc tgtctttgaa aagtgtctgg tcccaggaga
      841 gaaactcttt catattgcaa tattactttc actgcgcata aaataaatgt ttgctttatt
      901 taatcgtttg tattatatgt gcaaaaaata aatgacctat attaatcttg atcttaatgg
      961 ttctttttca taagtttagc ggaagactaa aatttcgatt tctactgatt ttctatagtc
     1021 tatatagttt cttgggataa catatatatt attcaaaacc agagtaacct atggtgataa
     1081 tgaaaatgaa aagaaaaatc atagtaataa tacttattct tgcaggcgtg ctgttagctg
     1141 gatgcgtaag caaacctgca gaagtgcagc ctacaacagt acaaccaaca aaagcccctg
     1201 agaccataac cttaatcggc gcaggtgcaa catttccata ccctctgcta tccaaatgga
     1261 gttcggaata taataagata aacccgaata tccagatcaa ttaccagagc gtcggaagcg
     1321 gagccggaat acagcagata actgcaagaa cagttaattt cggagccacc gatgcgccac
     1381 ttacggagca ggaattttca agtgtttcag ggatacttca aatacctgaa accactggtg
     1441 cagtggttgt agcatacaac ctgcccggaa tacagaaagg agttaaattg agcggtgatg
     1501 ttgttgcaga tattttcctc gggaagatca ataaatggaa tgatcccaga ataactttaa
     1561 taaaccctga catccagttc cctgataaag aaattatcgt ggttcacagg agcgatggaa
     1621 gcgggactac atttgtattc acggattacc tttcggcagt cagtcctgac tggaaatcaa
     1681 aggtgggaaa gggtaaatca gtcaactggc cggttggtct tggcgggaag gggaacgaag
     1741 gtgtttcagg tctcttgagc aagaacccta agagccagaa caattatacc ataggttatg
     1801 ttgagctggc tttcgctaaa ctccagaata tcccatatgc atatatcaag aacaaggccg
     1861 ggaaattcat cgagcccacg cttgaaacaa ccgcaagtgc agcagcaggc gccgtgccaa
     1921 atctacctgc aggtgatgcc agctggtcct ctgtgagtat agtggacgca cccggagaca
     1981 attcatatcc aataggcagc tttacatatc tccttgtcta caaagaccag actgataata
     2041 caaatggtaa agctcttgcc gaattcctct ggtgggcagt acacgacgga cagaaatatt
     2101 cctctgatct tctctatgtg ccgctgccag agcaagtcat tagccttaat gaaaagacca
     2161 taagacttat gaactataat ggacaaccaa ttatttaaat ggatggaaca aaattgtctt
     2221 taatccgttt cagccggaaa aagctctccg gcgattttat ttttgaagcc atagtaggat
     2281 tatttgctct tagcatattg atccttgcat tttttttatt cagggagctt tttatcggat
     2341 ccggactttc aagaaatact tttggcttaa gtttccttgt cacatctacc tggaaccctg
     2401 tgaatgagat attcggggct cttcctttta tcttcgggac tct
//
LOCUS       DATGBN010000452         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_269589, whole genome shotgun sequence.
ACCESSION   DATGBN010000452 DATGBN010000000
VERSION     DATGBN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269589"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VKL21_11500"
     CDS             complement(<1..379)
                     /locus_tag="VKL21_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF22 domain-containing protein"
                     /protein_id="HMB46433.1"
                     /translation="MNEIVNVVTHNNGAVNRKKVVASPYEFTIATRAKWEMIIADEDI
                     TIGAGKLERVKVKEIIVQKDMLAMPCAFSHHPIACVIKVKTINGPSPIETDRIVNSAF
                     VLGQESGEIKKGDLLSVLNLYPIM"
     gene            511..1464
                     /locus_tag="VKL21_11505"
     CDS             511..1464
                     /locus_tag="VKL21_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMB46434.1"
                     /translation="MPEKNHKYFAAHKEITSVARLSIYSNSLLLVMKLTVGIFMGSIS
                     ILSEALHSGIDLLAAIIANYSVKKAGQPADDEHRFGHGKFENVSGTVEAILIFVAAII
                     ILYKSSENILGNEGFEFEFIGIGIVVMGISAIVNFYVSARIMKVAKKAESIALEADAY
                     HLTTDVYTSIGVFIGLILIQITKNPVFDPLLAIIVALIILKASYDLTKRSVSGIMDVK
                     LSDKEEECIKSIIHEHYSRYAEYHNLRSRMSGAERFVDLHLVVPKNQLVVDAHDFCDH
                     LEKDIKEKIPNLSILIHIEPCKTDCEICKKLEVCTEPDIVH"
     gene            complement(1486..2145)
                     /locus_tag="VKL21_11510"
     CDS             complement(1486..2145)
                     /locus_tag="VKL21_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HMB46435.1"
                     /translation="MKFEEWEPIYKLILEDMQFDRIYDEKSARMLSKMLETKARKKIP
                     DIIEIEILRNAINGKDVLVCGKAPCLKYDIKKIDSGKYVVIAADGATSVLMNNGITPD
                     IIVTDLDGNMDDEAKANECGAIMVVHAHGDNTDALGEELPRLKRVIGTTQANPIINVY
                     NFGGFTDGDRCVFLAKEMGAKSIVLVGFDFEDKNVTPLKKKKLVWAKKLIGMVLGEGD
                     S"
     gene            complement(2247..>2680)
                     /gene="tfb"
                     /locus_tag="VKL21_11515"
     CDS             complement(2247..>2680)
                     /gene="tfb"
                     /locus_tag="VKL21_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324667.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HMB46436.1"
                     /translation="AVAALYSACRQCNVPRTLDEIGEVSRVSRKEIGRTYRFISRELG
                     LKLVPTSPIDYVPRFCSGLTLRGEVQSKAVEILRQASEKELTSGRGPTGVAAAAIYIS
                     SILCGDRRTQREVAEVAGITEVTIRNRYKELAEELDIEIIL"
BASE COUNT          751 a          587 c          482 g          860 t
ORIGIN      
        1 acattatagg atagagatta agcactgaca aaagatcccc tttttttatt tcccctgatt
       61 cctgtccgag aacaaaagcc gaattaacaa tgcggtctgt ttcgataggt gatggtccat
      121 ttatggtttt taccttgata acacatgcga tcgggtgatg actgaatgcg catggcattg
      181 caagcatatc tttctgcact attatttctt taactttaac tctctcaagt tttcccgctc
      241 ctattgtgat atcttcatcc gctattatca tttcccattt tgcccgtgtt gctatggtaa
      301 attcatatgg tgatgctacg acctttttcc gattcactgc gccgttgtta tgggtgacta
      361 catttacaat ttcgttcata atgaccccca taaaatagat aaaagtaata gtacttaaat
      421 tttatgatgg ttattaccat tatctgatat catcgatttt catgttgaca tatatacatt
      481 tatactatca cattattttg tataaaataa atgccagaaa aaaaccacaa atattttgcg
      541 gcccataaag aaataacatc cgtagcaaga ctttctatat attccaattc tttattactt
      601 gttatgaagc tcacagtggg cattttcatg ggttcgatca gtatattatc cgaagccctg
      661 cattccggta tcgacctttt ggctgcgatc atagccaatt attcggtcaa aaaagcagga
      721 cagcccgctg atgatgaaca taggttcgga catggtaaat ttgaaaatgt atccggaaca
      781 gtggaagcaa ttttgatctt cgtagcagca ataataattc tatataaatc aagtgaaaat
      841 atccttggta atgaaggttt tgaattcgag tttatcggaa tcggtattgt agtcatgggt
      901 atctcggcaa tcgtgaattt ttatgtatcg gcaaggatca tgaaagttgc aaagaaggct
      961 gaatccattg cccttgaagc agacgcttat cacctgacaa cggatgtata tacttctata
     1021 ggtgttttca taggtcttat tctcatccag atcacaaaaa accctgtttt tgatccgctg
     1081 cttgccataa tcgtggctct tataattctt aaggcatcgt atgacctgac aaaaaggtcc
     1141 gtatccggaa taatggatgt aaaactttca gataaagaag aagagtgcat taaatcaata
     1201 atccatgagc attattcccg gtatgcggaa taccataacc tgcgttcgcg aatgtcaggg
     1261 gctgaaagat ttgtggattt gcatctagtc gtgcccaaaa accagcttgt tgttgatgca
     1321 catgatttct gcgatcatct tgaaaaggat ataaaagaga aaatccccaa tctttcaata
     1381 ttgatccaca ttgagccatg caagactgat tgtgaaatat gcaagaaact tgaagtatgc
     1441 accgaacctg atattgtgca ttgacacttt ccaggaatta agcttttaag aatccccttc
     1501 acccagcacc atgcctatta gtttcttagc ccacacgagt tttttcttct taagaggagt
     1561 tacattctta tcctcaaaat caaatcccac aagaacgata gactttgctc ccatttcttt
     1621 tgcaaggaaa acacacctgt ccccatctgt aaatcctcca aaattataga cattgataat
     1681 gggatttgcc tgggttgttc cgattactct cttaagacgc ggcaattcct cacccagcgc
     1741 gtccgtgtta tcgccatgtg catgcactac catgatggcg ccgcattcgt ttgcttttgc
     1801 ctcgtcgtcc atattaccat caagatcggt aacgattata tccggagtaa tcccgttgtt
     1861 catcaggaca ctggttgccc catcagccgc aattacaaca tattttccag aatcaatttt
     1921 cttaatatca tatttaagac agggagcttt gccgcataca agaacatcct ttccatttat
     1981 cgcatttcga agtatctcta tctcaattat atcaggaatt tttttccggg cctttgtttc
     2041 aagcattttt gaaagcatgc gggcagattt ttcatcatat atcctgtcaa attgcatatc
     2101 ctcaaggatc agcttatata taggttccca ttcctcaaac ttcatataag aattactgac
     2161 attttgccaa tcaaaaaatc aaccggagaa gattaatctc ttcccggttt ttcaattctt
     2221 ccttattttc agatttcttt tactttctac aggataattt caatatccag ttcctcggcc
     2281 aattctttgt atcggttcct tattgtcact tcggttatgc ctgcgacttc ggcaacttca
     2341 cgctgtgttc tcctgtcacc gcaaagaatc gaggaaatat atattgccgc tgcggcaaca
     2401 cctgtggggc ctcttccgct tgtgagttct ttctctgacg cctgcctgag tatttcaaca
     2461 gcctttgact ggacttctcc gcgcagagtc aaacctgagc agaaacgagg cacataatct
     2521 ataggggagg tgggaacaag tttaagacca agttctcttg agatgaacct gtatgttctt
     2581 ccgatttcct tccggctgac ccttgaaact tcacctattt catcaagggt cctgggaaca
     2641 ttgcactgcc tgcatgctga atataatgcc gcaactgcaa
//
LOCUS       DATGBN010000453         7741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_307782, whole genome shotgun sequence.
ACCESSION   DATGBN010000453 DATGBN010000000
VERSION     DATGBN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307782"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VKL21_11520"
     CDS             <1..294
                     /locus_tag="VKL21_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB46437.1"
                     /translation="ALRSPLLDVDVSGATGALINVVGGPDMTIAEAESVVNELYTRID
                     SKARLIWGAMVDPDLENVIRTMVVVTGVTSPQILGKNTKGNIATAKYGIDFVR"
     gene            309..1037
                     /locus_tag="VKL21_11525"
     CDS             309..1037
                     /locus_tag="VKL21_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HMB46438.1"
                     /translation="MPAYINEIFNSIQGEGPYAGMRQVFVRFEKCQLHYSYCDTPQTR
                     EISDSCRIETKPGSGKFSCIQTPLQRDNVSDIIGKLWTNSTKHVSLTGGEPLLHADFI
                     RTLDIEYPLYLETNSGFPEKAREIKDIIAIASCDIKLPEHDCTDHYSELLKNELETIS
                     ILNETAKTFVKVIIMPDTTKKSLSFAIEVVKSIDKNIPFILQPVTSKNRVASSLLFEL
                     MDFAGEKLKDVRALPQTHKMMNLL"
     gene            complement(1034..1543)
                     /locus_tag="VKL21_11530"
     CDS             complement(1034..1543)
                     /locus_tag="VKL21_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46439.1"
                     /translation="MKKIIIILSVFLAAMVLISGCIDDKKSENPVNGTEIIESDQIPT
                     ANLPPGFTLMAVHETEADIGNSSRKAIEGVYRIDGDEIYIQVFNSETPEALIDELKSQ
                     YKDVKYDPFTEISINGHDATQVKYYIIKNGTQIPKYNVIWTTKNSLIKVGSSVDSQKV
                     IDLAEAIKS"
     gene            1634..2518
                     /gene="argB"
                     /locus_tag="VKL21_11535"
     CDS             1634..2518
                     /gene="argB"
                     /locus_tag="VKL21_11535"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HMB46440.1"
                     /translation="MLKREDILIEALPYIREFHDSIILIKMGGHAIVDPEIMEKIVQD
                     IVLLKFVGIHPVIVHGGGPEITEKMERMGKKPEFVAGLRITDDETLEIARMVLVGNVN
                     SKIVSLISRHGGKGIGLSGSDGRLIVAKKLALQRVTVGGVEKDIDLGWVGETEVINPE
                     IVMILAGKGYIPVISPIATDEKGNDLNVNADTVAGDIASAIGAKKLISLTDVPGILRD
                     PKDPKTRISRIAFNEIETLIAENIISGGMIPKIKSSASALKGGVRQVHIIDGGSPHSL
                     LIELFTNEGIGTMIYKKE"
     gene            2525..2869
                     /locus_tag="VKL21_11540"
     CDS             2525..2869
                     /locus_tag="VKL21_11540"
                     /inference="COORDINATES: protein motif:HMM:NF039857.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46441.1"
                     /translation="MTEIETKAETVPVIKILTPDERKRVLIEGIKKTVAPAFIGAFFA
                     VVFYFKFGHAQDVLWFSVFLLVLLLSYYIQRLVYPSIGVRVKEFEIKDWLYVELFTIV
                     FFWVFWTLLLNN"
     gene            2881..4647
                     /locus_tag="VKL21_11545"
     CDS             2881..4647
                     /locus_tag="VKL21_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HMB46442.1"
                     /translation="MRLAVLDRDRCQPKMCGLQCIKFCPRVRTGDETIIIGEDEKPVI
                     SEELCSGCGICVNRCPFGSIIIIGLPEKLEETTHRYGKNGFSLYGLPGPSIGKVTGIL
                     GPNGIGKSTAINILSGTLKPNLGRGRITWEEILEYYSGTALHDYLEGVSQKKVKTSQK
                     PQYVDMIPRKFKGKVSALLSKTDERGVLKDLISKLDISDIVDRKIDELSGGELQRVAL
                     AACVARDADFYFIDEISPYLDIYQRIKAAQIIRELASSRAVMVVEHDLAILDLLADVV
                     HVAYGTPGAFGVITHPKGVRIGINEYLKGFLREENVRIRTESIKFDVHAPQATKEVSS
                     LMTFGQFTNKYDKGFELIARGGEIRKGEVTGIVGPNGIGKSTFVKILAGEIQPTSGKI
                     DMNITVSYKPQYIKAEDPVPVSDYLHSLSKQVDSSYYQTEITRPLALERLMKQKLDDL
                     SGGELQRVAIAACLSRKADLYLLDEPSAHLDVEQRVQATKVIRRFAENNKVTAMVVDH
                     DIYMIDMLSDRLLVFEGEPMKRGEVHGPFDMREGMNRFLKGIGITFRRDEETKRPRVN
                     KLDSQLDRTQKAEGEYYYSVEK"
     gene            complement(4680..5318)
                     /locus_tag="VKL21_11550"
     CDS             complement(4680..5318)
                     /locus_tag="VKL21_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46443.1"
                     /translation="MMTLARLTSSGILLFLGTFAIIGIVLTSGCMEQSEQRTSGEVLG
                     DNNSVTPAPETDSTPAILSEKQILQLDIETPKIDSTPSILSEKQILQLDIDNAPYNIS
                     EYQENIFDCSNMAALLHDWLETQTQTKWNVEVWYGCESPSCREGHEWLEVIGNVSNFT
                     IEVTSKTIEDYWGNSNESYREHYSYFNRHFSFLPKGSGGFIDPKENIYPKRW"
     gene            complement(5375..6400)
                     /locus_tag="VKL21_11555"
     CDS             complement(5375..6400)
                     /locus_tag="VKL21_11555"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMB46444.1"
                     /translation="MTRDKHNIKFSKPIVSNKEGLQLSTPEIVAKYIARRLKTDIIAD
                     LGCGIGGQVIFFAKECKKVYAVERDAQKLEYARENCRLYGVDNVEFIPGDALSPEIKA
                     KVSDANIIFSDPARPLSEKERTLDNLEPPITEIIKIYSDVTLDLAFHAPPQMPPERIA
                     LDCEREYLSLNGQLNRLTLYFGSLKSCDRSAVVLPEGDNLCFSEAPEIRNSSLFDYVY
                     EPEPSVIKAGLLNELAQTLANENKDVFFYKGDERRTLLTSSTPVGSHFFKDSYRVRGS
                     TDMDMSKLKEILKSLKAKNVVLRFDIDPDKYWGIRTKLEEGLAGTRTIHVFGFGNELV
                     LCEKVRK"
     gene            6507..7253
                     /locus_tag="VKL21_11560"
     CDS             6507..7253
                     /locus_tag="VKL21_11560"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HMB46445.1"
                     /translation="MENDRTTVILSTATGMIALDSPVKLKILEILEIGTASFDELVEK
                     SNKAKSTISVHLHDLEGLHLIQEKTFPNDKRKKYYVLNAIYLAYSQRPLKKQYNRQMD
                     VIGTSIIDGNSFKEKLFENFCYGMEAYGINPKPILKRIGDDIGTKIGHELKSRDCEGI
                     LEELSVFWADHGLGDMTLLNSDQTGVSVINCYHCGKMPDVGKTLCSIDEGIIEGVLFS
                     KLKYDCRVREIECYGTGHNHCKFIVEEFEL"
     gene            7433..>7741
                     /locus_tag="VKL21_11565"
     CDS             7433..>7741
                     /locus_tag="VKL21_11565"
                     /inference="COORDINATES: protein motif:HMM:NF019320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HMB46446.1"
                     /translation="MKGAIVQCLGDLVKSSFGNDKWEQSLETAGMSKTSFFMPLQNVD
                     DEKVMKIIESLCKVANISLAQAADAFGDYWVNVYAPKLYSSYFKGANNSKELLLKMDS
                     V"
BASE COUNT         2384 a         1448 c         1652 g         2257 t
ORIGIN      
        1 gctctgcgaa gcccgctgct tgatgttgat gtatccggcg cgacaggcgc ccttataaat
       61 gtggtgggag ggccggatat gaccatagct gaagctgaaa gtgtggttaa tgagctttat
      121 acccgcattg attccaaggc aaggctgata tggggagcaa tggttgaccc tgacctggaa
      181 aatgtcataa ggacaatggt cgtagtcacc ggtgttacat cgccgcagat cctcggtaaa
      241 aatactaaag gaaatattgc tacggcgaaa tacgggattg attttgtaag ataataattg
      301 cgaaattgat gcctgcatac ataaacgaga tattcaattc tatccagggc gaaggtcctt
      361 atgcaggcat gcgtcaggtt tttgtaaggt ttgaaaaatg ccagcttcac tactcttatt
      421 gcgatacgcc acaaacacga gaaatttcag actcctgcag gatcgagacg aaaccgggaa
      481 gcggaaaatt ttcttgtatc caaacacctt tgcagagaga taatgtttca gacattattg
      541 gaaaattatg gaccaactca acaaaacatg tcagccttac cggtggagaa ccactgttac
      601 atgccgattt catacggacg cttgacatcg agtatccatt ataccttgaa actaattccg
      661 gttttcctga aaaagcaaga gaaatcaaag atatcatcgc aatagcatcc tgtgacatta
      721 aattgccaga acatgattgc acggaccatt attcagagct gttgaaaaat gagcttgaga
      781 cgatctcaat cttgaatgag actgcaaaga cttttgtgaa agtaataata atgcctgata
      841 ctacaaaaaa gtcattatcc tttgctatcg aagtagtcaa atcaatagac aagaatattc
      901 cttttattct tcagcccgtg acttcaaaaa accgggtcgc ttcatcattg ttgtttgaat
      961 tgatggattt tgccggggaa aaattaaaag atgtgcgtgc acttccccag acgcacaaga
     1021 tgatgaacct tctttagctt ttgattgctt cggcaagatc aattactttc tgggaatcaa
     1081 cagaggaacc tacttttatc aatgaattct ttgttgtcca gataacatta tattttggta
     1141 tttgagttcc attttttatt atataatatt tcacctgtgt agcatcatga ccattgatgg
     1201 aaatctcggt gaatggatca tatttgacat ctttgtactg tgatttaagt tcgtcaataa
     1261 gcgcctctgg agtttcagaa ttgaaaacct ggatatatat ttcatctcca tcgatcctgt
     1321 agactccttc aattgctttt cttgaggagt tgccaatatc ggcttcggtt tcatgtactg
     1381 ccatcaatgt aaaacctggg ggcaaattgg cagttgggat ttggtctgat tcaataattt
     1441 cagtcccgtt taccggattt tcgctttttt tatcatcaat acagccggaa ataagaacca
     1501 ttgcggctaa gaacacggat aatattatta ttattttttt catatttttt caaatatcct
     1561 tataggactt ggaattgaaa aatgtaacgg tcaggattaa cttttaatat ccgtatcccc
     1621 attttagcct tcgatgctca aacgagaaga tatcctgatt gaagctctcc catatatccg
     1681 tgaattccat gactctatca tcttgatcaa gatgggagga catgctattg ttgaccctga
     1741 aataatggaa aagatagtgc aggatatcgt tcttcttaaa tttgtgggaa tacaccctgt
     1801 aatagtacat ggcgggggac ctgagataac tgaaaagatg gagcgcatgg gaaaaaagcc
     1861 tgaatttgtg gctggattaa ggatcacaga tgatgagaca ctggaaattg caaggatggt
     1921 ccttgtggga aatgtgaata gcaaaatcgt ttcacttatc agcaggcatg gaggaaaagg
     1981 tataggcctc tcagggagtg acggacgact tatcgttgca aagaagctcg ctcttcagcg
     2041 tgtgactgtg gggggggttg agaaagatat agaccttgga tgggttggtg aaacagaagt
     2101 tatcaatccc gagatagtta tgatacttgc gggaaaagga tatatccctg tcatttcgcc
     2161 gatagccaca gatgaaaaag gaaatgacct taatgtgaat gcagatactg tagcgggaga
     2221 tattgcgtcg gctattggcg caaaaaaact tatttccctg acagatgttc ctggaatcct
     2281 acgtgacccg aaagatccca aaacacgcat ttcaagaata gcatttaatg aaattgaaac
     2341 acttattgca gagaacatca taagcggtgg catgatacct aaaatcaagt caagcgcttc
     2401 tgcgctaaaa ggaggggtga ggcaggttca tattattgac ggtggaagtc cgcattccct
     2461 gctaattgaa ttgttcacaa atgaaggcat agggactatg atatataaaa aagagtgaat
     2521 tattgtgact gaaatagaga caaaagcgga aacagtaccg gttatcaaga tcttgacccc
     2581 ggatgagcga aagcgagtat tgatagaagg aataaaaaaa actgttgcac ccgcatttat
     2641 cggtgcgttc tttgcagtag tattttactt caaattcggt catgcgcaag atgttttatg
     2701 gttttccgta ttcttgctgg tattgcttct ctcatattac atccagagat tagtttatcc
     2761 ttcgattggc gtcagggtga aggaatttga aatcaaggac tggctttatg tggaattatt
     2821 taccatcgtt ttcttctggg ttttctggac gcttttattg aataattgaa ttgaggtatt
     2881 atgcgacttg ctgttttaga ccgtgacagg tgccagccca agatgtgtgg gctccagtgc
     2941 attaagttct gccccagggt aaggacaggc gatgagacta taataatagg tgaagatgaa
     3001 aaacctgtca tctcggaaga attatgctca ggatgcggaa tatgtgttaa ccgttgccct
     3061 ttcggttcaa taataataat aggccttccg gaaaaacttg aagagacaac tcaccggtat
     3121 ggtaaaaatg gtttttcatt atacgggctt cccggacctt cgatagggaa agtcacgggc
     3181 atccttggcc caaatggcat tggaaaaagt actgcgatta acatactttc cggaacattg
     3241 aaaccaaacc tggggcgtgg aaggatcacg tgggaagaga tacttgaata ttactcaggg
     3301 accgctcttc atgattacct tgaaggcgta tcacaaaaaa aagtaaagac ttcccaaaaa
     3361 ccgcaatatg tggatatgat ccccaggaaa ttcaaaggaa aggtatctgc gttattatcc
     3421 aaaacagatg aaagaggggt acttaaggac cttatatcca aacttgatat ttccgatatc
     3481 gtagatagaa agatcgatga gctaagcgga ggtgaactcc agagagttgc gcttgccgct
     3541 tgcgtggcaa gagatgcgga tttctatttc attgatgaga taagccccta ccttgatatt
     3601 taccagagga taaaggcggc acaaatcatc agggaacttg catccagcag ggccgtaatg
     3661 gtcgtggaac atgaccttgc catcctggac ctgcttgccg atgttgtgca tgttgcatac
     3721 ggcacgcccg gcgccttcgg tgtaattacc catccaaaag gggttcgcat cgggataaat
     3781 gaatatttga aagggttcct gcgcgaagaa aatgtgcgta tcaggacaga atccataaag
     3841 ttcgatgtcc atgcgcccca ggctacaaaa gaagtatcat cccttatgac cttcggacaa
     3901 ttcactaata aatatgacaa gggttttgaa ctcatagcca ggggcggaga aataagaaag
     3961 ggtgaagtca caggaattgt ggggccaaac ggaataggta aatccacttt tgtcaagatc
     4021 cttgccggag agatccagcc cacaagcggt aagatagata tgaatattac cgtatcctac
     4081 aaaccccagt atatcaaggc cgaagaccct gtaccggtat cggattacct gcactccctt
     4141 agcaaacagg tggattcgag ttattaccag accgagatca caagaccgct tgcgcttgaa
     4201 cggctcatga aacaaaagct tgatgactta agcggaggtg aactccagag agttgcaata
     4261 gcagcgtgcc tttcccggaa ggcagacctg tatcttctgg atgaaccatc ggcgcatctt
     4321 gatgttgagc aaagggtaca ggcgacaaaa gtcatcagga gatttgccga gaataacaag
     4381 gttacagcta tggtcgttga ccatgatatt tatatgatag atatgttgag cgacaggctt
     4441 cttgtttttg agggggaacc catgaaaagg ggcgaggtac atgggccttt tgatatgcga
     4501 gaaggcatga acaggttctt aaaaggcatc gggataacat tcaggcgtga tgaggagaca
     4561 aagcgaccca gggtcaataa gcttgattcg cagcttgaca ggacgcagaa ggcagaaggg
     4621 gagtattatt acagtgtgga gaaatagttg attagttctt ttgggaggtt gccacagttt
     4681 caccatcttt ttggatatat gttttctttt gggtctataa atcctccgga gcctttaggc
     4741 aaaaaactaa aatgtctatt gaaataagaa taatgttctc tataactttc attggagttt
     4801 ccccaatagt cttctatcgt ttttgatgtt acttcaattg taaaattaga tacatttcca
     4861 attacttcaa gccattcgtg accttctctg caggatggag attcacaccc ataccacact
     4921 tccacattcc actttgtttg cgtttgagtt tctaaccaat catgaagtag agccgccata
     4981 ttggaacaat caaatatatt ttcttgatat tcgcttatat tataaggggc attatctata
     5041 tccaattgca atatttgttt ttcagataaa atagatggag ttgaatcaat ttttggtgtt
     5101 tctatatcta attgcagtat ttgtttttca gataggatag ctggagttga atctgtttct
     5161 ggtgctggtg ttacggaatt attgtctcct aaaacttctc cggatgttct ttgttcactc
     5221 tgttccatgc atcctgatgt caaaacaatt ccaattattg caaatgttcc aaggaataat
     5281 aatattcctg aagatgtcag tctggccaga gtcatcattt ctatataacc atattttttt
     5341 taatataata atatatcttt tcctgacatg agcatcattt cctgaccttt tcgcacaata
     5401 caagctcatt cccgaatcca aatacatgaa tcgtccttgt gcctgccagt ccttcctcaa
     5461 gttttgtccg tatcccccag tatttatcag gatcgatatc aaaccgcaat acaacatttt
     5521 ttgccttcag ggattttaat atctctttta atttcgacat gtccatatct gtacttcctc
     5581 tcactctgta actatcctta aaaaaatgtg atccaacagg cgtcgatgaa gttaaaaggg
     5641 tgcgcctctc atcaccctta taaaaaaata catctttatt ttcatttgcg agggtctgcg
     5701 ccaattcatt caggagcccc gctttaataa cagaaggctc aggttcataa acataatcaa
     5761 aaagtgaact attcctgatt tcaggcgcct ctgaaaaaca gagattatcg ccttcgggaa
     5821 gaacaacagc acttctgtcg caggatttga gcgatccaaa atataatgtc aaccggttca
     5881 attgcccgtt caatgaaagg tactcgcgtt cacaatcaag cgctatccgt tccggaggca
     5941 tctggggcgg tgcatgaaat gccaggtcga gtgtgacatc cgaatatatt tttattattt
     6001 cggtaatcgg cggctcaaga ttatcaagag tcctttcttt ctcagagaga ggacgggcag
     6061 gatcggaaaa aatgatattc gcatcagata cttttgcttt tatttcagga gataatgcat
     6121 cacccgggat gaattcaaca ttatcaacac catataacct gcaattttct cttgcatact
     6181 caagcttttg ggcatctctt tccaccgcat agactttctt gcactccttt gcaaaaaaaa
     6241 taacctgtcc cccgatcccg caacccaggt ctgcgattat atccgtcttt aacctcctgg
     6301 cgatgtattt ggctacgatc tccggagttg aaagttgtaa accttcttta tttgaaacga
     6361 tcggttttga aaatttaata ttatgtttat cacgggtcat aatctttcat aatatcgacg
     6421 cattcatatt tatgttatat cccggaaaga aaaaagtttc gtacataaac gaacattatt
     6481 tatataacta aacctattta gatagcatgg aaaacgaccg gactacagta atcctatcta
     6541 ctgctactgg tatgatagct ctggacagcc cggtcaaatt gaagatcctg gaaatccttg
     6601 aaataggcac tgcatcattt gatgaacttg tggaaaaatc caataaagca aaatccacta
     6661 tatccgtgca tcttcatgat cttgagggat tacatcttat ccaggaaaaa acctttccga
     6721 atgataagag aaagaaatat tatgttttga atgcgatata tcttgcttat tcacagagac
     6781 ctttgaaaaa acaatataac aggcaaatgg atgttatcgg cacatcgata atagatggta
     6841 attcttttaa ggaaaaactt ttcgaaaatt tttgctatgg catggaagcg tatggtataa
     6901 acccgaaacc tattttgaaa aggattgggg atgatattgg cacaaaaatt ggccatgaat
     6961 taaaatcccg tgattgtgag ggcatacttg aggaactatc agttttttgg gcggatcatg
     7021 gccttggtga tatgacctta ttaaatagcg atcaaacagg agtttcagtt atcaattgtt
     7081 atcattgtgg aaaaatgcca gatgttggca agaccttgtg ttccattgat gaagggatta
     7141 ttgaaggggt attattcagc aaattgaaat acgattgcag agtaagagaa atagaatgtt
     7201 atggaacagg gcataatcac tgcaaattca tcgttgaaga attcgaactt taatagatag
     7261 aactttcaat ctttttttat agattacaat acttttgtaa tcttaaagat agttagatat
     7321 atataccaag gaaagtatga taattgtcat aatcatgata atttgccgat aagatcttca
     7381 aattatctat tgaaatagaa actaaaagaa aacaaaaatg gaggatcaaa aaatgaaagg
     7441 tgctatagtc caatgtttag gagatctcgt aaaatcaagt tttggaaatg ataaatggga
     7501 acaatccctg gaaacggcag gtatgagcaa gacatcattt ttcatgccgc ttcagaatgt
     7561 agatgatgaa aaagtgatga aaatcataga atcattatgc aaggttgcta atatatcact
     7621 tgcacaggca gcagatgcat tcggtgatta ttgggtaaat gtatatgcac ccaagttata
     7681 cagttcctat tttaaaggag ctaataattc aaaagaactt cttttaaaaa tggatagtgt
     7741 a
//
LOCUS       DATGBN010000454         6858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_424599, whole genome shotgun sequence.
ACCESSION   DATGBN010000454 DATGBN010000000
VERSION     DATGBN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424599"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VKL21_11570"
     CDS             <1..763
                     /locus_tag="VKL21_11570"
                     /inference="COORDINATES: protein motif:HMM:NF018546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HMB46447.1"
                     /translation="NMLDILKILFVTPFLVYSCYTDIKTRRVTNKLWQAMLAGGSFFI
                     IYDILVYGFDYLFKIFISTALIFIFVYLLFQLGVFGGADAKSLIVLSLFFPAYPELTI
                     SGYDFPINRPLPLFTDFFAFGVFENAVLLTIVVPLSLAIYNVSKMGLHIDKPLYAFIG
                     YKTRISELSNKKHIKMIEGFEKVNDSINFYFKRGGLEIDDKVLKELNILSKKGLVKDI
                     WVTPGLPFMIPITLGFYVSIFYGDLITELTKYLLL"
     gene            861..1382
                     /locus_tag="VKL21_11575"
     CDS             861..1382
                     /locus_tag="VKL21_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HMB46448.1"
                     /translation="MARKPARMYKKITQRSYTRREYMGGVPGSKIVTYDMGNLTEDFP
                     IQLTLVTKEECQIRHNALESARIAANRILLDTLGQPNYHLKIRVYPHQVLRENKQATG
                     AGADRVSQGMRLAFGKAIGTAARVRRGQEIMSVFVTAANFKLAKKSLISAGYKLPTPI
                     SLVVEKGQELVLT"
     gene            1426..2046
                     /locus_tag="VKL21_11580"
     CDS             1426..2046
                     /locus_tag="VKL21_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HMB46449.1"
                     /translation="MNDYLANLDRALKHLPEVKSSGERFVVPEPKLLTEGKTTVLENF
                     AVIVDKLNREQSHIFKFLLRELGTAGKIDGSRAIFMRRLTTGMISELINAYVKEYVTC
                     SECGRPDTHLIKSDRVLTLRCDACGAHRPVTKRRATSIAKEEALSEGESYDVRIDAVG
                     SKGDGIAKKDKYTIYVPNTVKGDTVKIKIKKITGNLAFAEMLEKIT"
     gene            complement(2202..3392)
                     /gene="glmU"
                     /locus_tag="VKL21_11585"
     CDS             complement(2202..3392)
                     /gene="glmU"
                     /locus_tag="VKL21_11585"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sugar-1-phosphate
                     nucleotidylyltransferase/acetyltransferase"
                     /protein_id="HMB46450.1"
                     /translation="MKAVILVAGQGTRMGSLTLNTPKVMLPIANKPILFYVIQSARDA
                     GIRDFVLVVGYGAKLIKDYFKDGSFMDVGIEYVHQEKQMGTADAVASAKGIVGDRFLV
                     LNGDIIVNPEHIKKLIQCRSDAVMTARHVDNPSEYGILEVRNDHVLRIIEKPVVPATD
                     LANAGIYLFPSSIFDAIRKTPLSVRKEYEITDSLQMLIDNGTDVGFLELSDKWMDIGR
                     PWDLLDANEYFLSGLQPVTSGEIEPLATLKSSVSVGEGTIIRNGSYIVGPVIIGKNCD
                     IGPNCYIRPGTSIGDNVRIGNAVEIKNSIIMKGTHIGHLSYVGDSIIGERCNFGAGTK
                     VANLRHDGRTIRVTLKGKIMDSGRKKLGTIMGDDVHTGINSMINVGAVIESKAMIAPG
                     ELIK"
     gene            complement(3389..4681)
                     /gene="glmM"
                     /locus_tag="VKL21_11590"
     CDS             complement(3389..4681)
                     /gene="glmM"
                     /locus_tag="VKL21_11590"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMB46451.1"
                     /translation="MALFGSSGIRGLANKEITPELALSVGLAVGSLHGSAVIARDPRT
                     SGEMIEHALISGLLSAGCSVTRVGMVPTPTLAYAARNFDCGIMITASHNPPEYCGIKL
                     WNRDGMAFDSRRQDEIESIIRDRTWKTAQWQNIARVWEADVIEDHAVMILSKVGHASL
                     KVVVDCGCGAASVITPYVLRRMGCTVITLNSQPDGFFPARNPEPIEKNLELLKKATLS
                     FGADIGIAHDGDADRMMAIDPGGRFIEGDKLLAFFGIRETRHSIVVPVDTSIMVDDVL
                     KNRKVYRTRVGDVYVAEELKKRNAEFGGEPSGSWIFPEISLCPDGILAAAKLVEMVQN
                     EGRLDVLIDELPAYPMIRGAVPCSNEKKETAMKKIAVSLRKIGEVTDIDGIRLETENG
                     WILVRPSGTEPKIRITAEARKGVEELFKKAHKLVSDSL"
     gene            complement(4684..6498)
                     /gene="glmS"
                     /locus_tag="VKL21_11595"
     CDS             complement(4684..6498)
                     /gene="glmS"
                     /locus_tag="VKL21_11595"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMB46452.1"
                     /translation="MCGIVGYVGTRPATPILIDALKRLEYRGYDSAGLAILEDNGICV
                     CKTEGKISDLESKLPRIKGTCGIGHTRWATQGAPTTINAHPHVSGNIAVVHNGIIENY
                     QELYDWLKEEGYEFVSETDTEVLAHLAHKYYEGDLVKAVIEGLKKVKGSYAAAYLCSD
                     SKMVVAAKKESPLVAAVGKGENFVASDVTALLKNSRDVIYMQDFEVVRLTGEDMQFFD
                     FNGDPVQKKIESIEWNIEAAEKSGYPHFMLKEIHEQVTSVHDTLLGRISELSGEVILG
                     EVTLSNDEIRDLDRIVIVACGTSYNAGLLGRYLFESMAGIHTDVEISSEFRYANPVLA
                     GSTLVIAISQSGETADTIAATREAKTYGCHTLAITNVVGTTLSREVENIVYTRAGPEI
                     GVAATKTFTSQLIALYLLSIYFARKRKKMDVDRSKRLLVSMKQLPGMIQQILNNKEFI
                     KKQAIRFSTARDYFFLGRSLNFPIALEGALKLKEISYIHAEGYAAGELKHGPLALISK
                     ETPVLALATRSSTYDKMVNNIKEVKARSAQVIAIACMDDTEIEKYVDVVIRVPMADEL
                     LYPVLSSVVVQLLAYNSADALGCAIDKPRNLAKSVTVE"
     gene            complement(6508..>6858)
                     /locus_tag="VKL21_11600"
     CDS             complement(6508..>6858)
                     /locus_tag="VKL21_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HMB46453.1"
                     /translation="PSTAIGDNCSISSFTEIENSIVMNDVRIGAGSYISNSIIGSNNS
                     IGPHFIAEVGKDLKIEMKGMLHSADELGTVIGDSNLIGPCVLVRAGKMIAMGCSVDSG
                     VRVTKDIPKDSRVL"
BASE COUNT         1839 a         1630 c         1378 g         2011 t
ORIGIN      
        1 gaatatgctg gatatattaa agatattgtt cgttacgcct tttttagtct attcgtgcta
       61 tacggatatt aagacccgcc gtgttacaaa taaactatgg caggcgatgc ttgcgggtgg
      121 atcttttttc atcatatatg acatcctggt ttatgggttt gattaccttt tcaagatctt
      181 tatttcgaca gcattgattt tcattttcgt gtatctctta tttcaacttg gcgtatttgg
      241 aggggctgat gccaaatccc ttatcgtcct atctttattt tttcctgcat atccggaatt
      301 aacaatatca ggttatgatt tcccgataaa caggcctttg ccgcttttta ctgatttctt
      361 tgccttcggg gtatttgaga atgcagttct tctcactatc gttgttcctt taagcctggc
      421 tatttataat gtttcaaaaa tgggtctaca tatcgataaa ccgttgtatg cgttcatagg
      481 ttataaaacc cgcattagcg aactttccaa taaaaaacat attaaaatga tcgagggttt
      541 tgaaaaggta aatgatagta tcaattttta ttttaaaaga ggcggcctgg aaatcgatga
      601 taaggtctta aaggagctta atattttgtc aaagaagggc cttgtcaagg atatatgggt
      661 cacacccggg ctgccattta tgataccaat aacattgggt ttctatgtat cgatatttta
      721 tggcgatttg ataacagaat taacaaagta tcttctttta tagcaatagt tataaagggg
      781 atattactat gttaagcaga caaattagac ataatttatc agattattgt aaatttttat
      841 actaattgga ggattattca atggcaagaa aaccagcaag aatgtacaag aagatcaccc
      901 agcgctcata tacaaggcga gaatacatgg gtggcgttcc gggcagtaag atagttactt
      961 atgacatggg caatttaact gaggatttcc cgatccagtt gacgctcgtg acaaaagaag
     1021 aatgccagat ccggcacaat gctcttgaat cagcacgtat tgcagctaac agaatattgc
     1081 ttgacacttt aggacagccc aattatcatt taaagataag ggtatacccg catcaggtcc
     1141 tgcgcgaaaa taaacaggct acaggagcag gggcagaccg tgtatcgcag ggaatgagac
     1201 tggcattcgg aaaagctatc ggaactgcgg caagggtgag aagaggccag gagatcatga
     1261 gcgtatttgt tactgctgcc aatttcaaac ttgcaaagaa atcattgatc tcagctggat
     1321 ataaattacc gactccgata agccttgttg ttgaaaaagg ccaggaactg gtccttactt
     1381 gatcttaaat gaagacttcc gcaaaaatgc aggggttatt gctttatgaa tgactatctg
     1441 gcaaaccttg acagggcatt aaaacatttg cctgaagtta aaagttcagg ggagcgtttt
     1501 gttgttcctg aacctaaact ccttacagag ggcaaaacaa cagtacttga gaattttgca
     1561 gtgatagtgg ataaattgaa ccgcgagcaa tcgcatattt tcaaattcct gctcagggaa
     1621 ctgggaacag ctggcaagat agatggttca agggcgatat tcatgagaag attgacaaca
     1681 ggcatgatct cggaactcat taatgcatat gtcaaagaat atgtaacctg ctcagaatgc
     1741 ggacgtcccg atacccacct tatcaagagt gacagggtac tcacattaag atgcgatgca
     1801 tgcggtgcgc acaggcctgt aacgaaacgc cgggcaacat ctatagccaa agaagaagcg
     1861 ctgtccgaag gcgaatccta tgatgtcaga attgacgctg tgggaagcaa gggtgacggc
     1921 attgccaaaa aggataagta tacgatctat gtgccaaaca ccgtcaaagg ggatactgta
     1981 aaaataaaga tcaagaagat cacaggcaat cttgcttttg cggaaatgct ggaaaaaata
     2041 acataggact gaaaatcaag gcacatcgcc cggccttggt ttaagagccg ttattattgt
     2101 tgccatactt gcggcgactg cgattctcgg agcgatgtgg gcttcttggg gcattaaata
     2161 cgggtagaga atgcggctgc aaatgattga gccattacga tttattttat taattccccc
     2221 ggcgctatca tcgctttgct ttcaataaca gctcccacgt ttatcatgct gttaatacct
     2281 gtatggacgt catcccccat gatcgttcca agctttttcc ttcctgaatc cataattttt
     2341 ccttttaatg tcactcgaat tgtcctgccg tcatgcctca ggttcgcaac ctttgttccg
     2401 gcaccgaaat tgcatctttc tccgattatg ctgtcaccca cataactcag atgccctata
     2461 tgcgtccctt tcatgataat gctgttcttg atctcaaccg catttccaat gcgcacatta
     2521 tccccgatac ttgtaccggg tcggatatag caattggggc cgatatcaca attctttccg
     2581 ataatcacag gtccgactat gtatgagcca ttccttatga tcgtgccctc tcccactgaa
     2641 acactacttt ttaaggtcgc aaggggctct atttcaccag aagtcacagg ttgtaaacct
     2701 gataaaaaat attcattggc atcaagaagg tcccagggtc tgccgatatc catccattta
     2761 tcggaaagtt ccagaaaacc cacatccgtt ccattatcga tgagcatctg gagcgaatct
     2821 gttatctcat attcttttct taccgaaagt ggggttttgc ggatagcatc gaaaatcgat
     2881 gatggaaaaa gatatattcc tgcatttgca agatcggttg ctggaacaac gggcttttca
     2941 atgatccgca aaacatggtc attccggact tcaaggatgc catactcgga aggattatca
     3001 acatgcctcg ctgtcattac agcatctgac ctgcattgta tgagcttctt tatatgctcc
     3061 ggattcacaa ttatatcccc attcaatacc aggaagcgat cgccaacaat tcccttcgca
     3121 gaggcaacag catcagcagt gcccatctgt ttttcctgat gaacatattc tatgcccaca
     3181 tccataaaag agccgtcttt gaaatagtct ttaataagtt ttgccccata gcctacaaca
     3241 agcacaaaat ccctgatccc cgcatcccgc gctgattgga taacataaaa aagaataggt
     3301 ttgttcgcta ttggcagcat gacttttggc gtattaaggg ttaacgagcc catccttgtg
     3361 ccctggcccg ccacaagaat tacagccttc aaagtgaatc acttaccagc ttgtgcgctt
     3421 ttttaaacaa ttcttctact ccctttctgg cttctgccgt tatcctgatc tttggctcag
     3481 tcccgctggg gcgcaccagt atccacccat tttccgtttc caatcttatt ccatctatat
     3541 ctgttacttc tcctattttc cttaaagaaa cggcgatctt tttcattgct gtttctttct
     3601 tttcattact gcacgggaca gctccccgta tcataggata tgctggaagt tcatctatca
     3661 gaacatcaag ccttccctca ttctgcacca tctcaacaag ctttgctgcg gccaggatcc
     3721 catccgggca cagggaaatt tccgggaata tccagctgcc tgagggctcg cccccaaact
     3781 ctgcatttct ctttttcaat tcttccgcaa catatacatc gccaacacga gttcgataga
     3841 ccttccggtt tttaagtacg tcatcgacca taatagaggt atcaacaggc acaacgatac
     3901 tatgtcttgt ttcacggatc ccgaagaaag caagaagctt gtccccttct atgaacctcc
     3961 ccccggggtc aattgccatc atcctgtccg cgtctccgtc atgcgctatg ccaatatcgg
     4021 ctccaaatga aagagtggcc tttttcaata gttccaggtt cttctcgata ggttcgggat
     4081 ttcttgcagg gaagaatcca tctggctggg aattaagagt aatgaccgta catcccatgc
     4141 gcctgagtac ataaggagtt attacggaag ctgcgccgca tccgcagtca acgaccactt
     4201 tcaaggaagc atgtcccacc ttactgagta tcatcactgc atggtcttca attacatctg
     4261 cttcccagac ccttgcgatg ttttgccatt gtgcagtttt ccatgtccta tcccggatta
     4321 tcgattcgat ctcatcctgc cgcctggaat caaacgccat accatccctg ttccagagtt
     4381 ttattccgca gtattctgga ggattatgcg atgccgttat catgataccg cagtcaaaat
     4441 tcctggcagc atatgcaaga gtgggcgtag gaaccattcc gacccttgta acactgcatc
     4501 cggctgacaa cagccctgag atcaaagcat gctcgatcat ttctcctgag gttctcgggt
     4561 ccctcgcgat cacagctgat ccatgaagcg aaccaactgc aagcccgacc gaaagtgcaa
     4621 gttccggagt tatttcctta ttagccaggc ctcttatccc tgatgaacca aataaagcca
     4681 tatttattcc accgttacgc tttttgcaag gttcctgggt ttatcgatcg cacaacccag
     4741 tgcatcagcg ctgttgtaag cgagaagctg gacaacgaca gatgatagaa ccggataaag
     4801 caattcatcc gccatcggta cccttataac tacatccaca tatttttcta tttccgtatc
     4861 atccatgcat gcgatagcta ttacctgtgc gcttcgggct tttacttctt tgatgttatt
     4921 caccatttta tcgtaagttg aagagcgggt agcaagcgcc aacaccgggg tctcctttga
     4981 aataagagcc agcggcccat gtttcaattc tcctgcagca tatccttcag catgaatata
     5041 ggatatctcc ttgagcttca atgcgccttc aagggcgatg ggaaaattca gggacctgcc
     5101 taaaaagaaa taatcccgcg ctgttgagaa ccggatagcc tgtttcttga tgaattcctt
     5161 gttattcagg atctgctgga tcatgccagg gagctgtttc attgagacaa gcaaacgttt
     5221 tgacctgtcg acatccattt tttttctctt cctggcaaaa tagatggaaa gcaaatacag
     5281 ggcaataagc tgggaagtaa aagtctttgt cgccgcaacg cctatttcag ggccagctct
     5341 tgtataaaca atattttcca cttcacggga aagcgttgtt cccactacgt tagttattgc
     5401 aagggtatgg catccgtagg tttttgcttc tcttgttgct gctattgtat ctgcggtctc
     5461 acctgattgc gatatggcta ttacaagcgt tgatccggca agaaccggat ttgcatacct
     5521 gaattctgaa cttatttcca catccgtatg tatcccggcc attgactcga aaagatatct
     5581 tcccagaagc cctgcattat atgatgtgcc gcaggcgact atcactatcc ggtcaagatc
     5641 ccttatctca tcattggaaa gcgtgacctc gccaagaatt acctcaccgc tgagttcgga
     5701 tatcctgccc aaaagcgtat cgtgcactga tgtaacctgt tcatggatct cttttagcat
     5761 aaaatgcgga tatcctgatt tttcggctgc ttcgatattc cattctattg attctatttt
     5821 cttttgaaca ggatctccat taaaatcaaa gaactgcata tcttccccgg tcaaccgaac
     5881 cacctcaaaa tcctgcatat agatcacatc cctggaattc ttcagtaatg cagtaacatc
     5941 cgaagctacg aaattctcac cctttcccac ggcggcaaca agagggcttt ctttttttgc
     6001 tgctacaacc atctttgaat cgctgcataa gtaggctgct gcgtatgagc ctttgacttt
     6061 cttcaggcct tctatgacag ctttaacaag atctccctca taatacttgt gagcaaggtg
     6121 cgcgagaacc tcagtatctg tttccgaaac aaattcatat ccctcttcct ttaaccagtc
     6181 atacaattcc tggtagttct caataattcc attatggaca actgcgatat tgccggaaac
     6241 atgaggatgc gcatttatgg tggttggcgc cccctgcgtt gcccaccgtg tatgtcctat
     6301 gccgcatgtc ccttttatcc ggggaagttt tgattcgagg tcagatattt tcccttctgt
     6361 tttacatacg caaatcccat tatcttctaa aatggcaaga cctgcagaat catatcccct
     6421 gtactcaagt ctttttaatg catctatcag gatcggagtc gccggtctgg ttccaacata
     6481 tcctactatg ccgcacattt ttttacctca aagcaccctc gagtccttgg gtatgtcttt
     6541 tgtaaccctg actccggaat caacggaaca acccattgcg atcatttttc cagctcttac
     6601 aaggacgcat ggccctatca gattactatc cccaattaca gttcccagtt catcggcgct
     6661 atgcagcatt cctttcattt ctatcttcag gtcttttccc acctcggcaa taaaatgagg
     6721 accgatggaa ttattgctac caataatgga atttgagata tatgaacccg cgcctattcg
     6781 cacatcattc atgacaatac tattttcaat ctctgtgaat gaacttattg aacaattatc
     6841 tccaatagct gttgaagg
//
LOCUS       DATGBN010000455         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_152974, whole genome shotgun sequence.
ACCESSION   DATGBN010000455 DATGBN010000000
VERSION     DATGBN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152974"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VKL21_11605"
     CDS             <1..438
                     /locus_tag="VKL21_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB46454.1"
                     /translation="VLKSVVVIAATNRPEIIDPALIRSGRFDRLVFIGPPGKAGRVSI
                     FNIHLKNLKDKHLCEDVDIDELADFTENYVGSDIESLCREAVMLALRENFETDIVEMR
                     HFNEALKKVRPALADDMVEYYQKLQEQFKGGTKQEQKSYIGYR"
     gene            500..763
                     /locus_tag="VKL21_11610"
     CDS             500..763
                     /locus_tag="VKL21_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HMB46455.1"
                     /translation="MAKILAKNLANKRVMLTDGSELGFATDVVMDMQGGALIYLVVKP
                     TNNVDISKYKTQNNYVLIPFEAVRAAKDYAIVDKKMITGSDFD"
     gene            803..1966
                     /locus_tag="VKL21_11615"
     CDS             803..1966
                     /locus_tag="VKL21_11615"
                     /inference="COORDINATES: protein motif:HMM:NF016312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMB46456.1"
                     /translation="MKGESKVTIVCSIKDRASQNIKNSIFYLRKWDTLPEENIPVFEY
                     KDFRLVEIGESLIFQDGLDKKLSALGYPASLLIFASKHQSKDMRPILTVHSTGNVNEA
                     KFGGAPKTLSYAAPQAVRSLLRSLKMLAENEEYEVTLECTHHGPSDLDIPSVFIEVGS
                     NETQWLDDVAGRIVAEAILLLKDSDSPVPSHALDELDEILISQAFLRSGADFAYCMQL
                     RKRVKEICPSGKTVITEGIKCALSSCQTCICPGVKIARISPGLLSEAEKLDKNGLKVF
                     LSDHNIAYIEYEDGRFAHILIGLDDSCARLAAEDLRDKCVEIIKKHYDVCLDKGILQI
                     SERKFSPKLAKSFGIEDLAFYGKLARGESVIIDGKTINPEMVYETNKRAIKLN"
     gene            1986..>2797
                     /gene="ftsZ"
                     /locus_tag="VKL21_11620"
     CDS             1986..>2797
                     /gene="ftsZ"
                     /locus_tag="VKL21_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB46457.1"
                     /translation="MESIIKEAISRVGESPMPIIGDTDRELLEILQELKTNIVVIGCG
                     GSGSNTIQRIYNEGIIGAELYALNTDAQHLLNIKVKKKILIGRNRTKGLGAGSLPAIG
                     QDAANESKPAIEKAVGNADLVFITCGLGGGTGTGSAPIVAEIARDAGALTIAVVTLPF
                     SVEGQIRMDNAEAGLERLRDAVDTVIVVPNDKLLEVVPHLPLQSAFKVCDEVLMRSVK
                     GITELITKPGLVNLDFADVRVIMQKSGVAMIGLGEAEGENKAIESVQKALRSP"
BASE COUNT          901 a          514 c          675 g          707 t
ORIGIN      
        1 gtcctcaaga gtgttgttgt tatcgctgca acgaacaggc ctgagataat tgaccctgcc
       61 ctgatccgtt ctggcaggtt tgacaggctc gtttttattg gacctccagg gaaagccgga
      121 agagtcagca tattcaatat ccatcttaag aacctgaaag acaaacacct ttgcgaagat
      181 gtagatatag acgaactggc tgacttcact gaaaattatg tcggctctga catcgaatct
      241 ttgtgcaggg aagctgtaat gcttgctctt cgggaaaatt tcgaaacgga catcgttgaa
      301 atgcggcatt tcaatgaagc acttaagaaa gtaagacccg ctcttgcaga tgatatggtg
      361 gaatactatc aaaaactaca ggagcaattt aaaggcggga caaaacagga acagaagtct
      421 tatataggtt acagatgaaa aaagcataat agatgagccc atcataagga agaaaatttg
      481 ggctaattaa gggattacta tggcaaaaat acttgcaaaa aatttagcta ataagagagt
      541 gatgcttacc gacggatcag agctgggatt tgctacggat gtagtaatgg atatgcaggg
      601 tggagctttg atatatctgg ttgtaaaacc aacgaacaat gtagacatat caaaatacaa
      661 aacacaaaac aattatgtgt taataccatt cgaagcagta agggccgcca aggattatgc
      721 catagtggat aagaagatga tcactggcag cgactttgat taatatttta tctattccat
      781 tcgggcatta gaaggcagtg ttttgaaagg ggaatcaaaa gttacaatag tctgttcaat
      841 caaggacagg gcaagccaga atattaaaaa ttccattttc tatcttcgaa aatgggatac
      901 ccttcctgaa gaaaatatcc ctgtttttga gtataaggat ttcagattag tggaaatagg
      961 ggaatcccta atttttcagg atggccttga taagaagtta tccgcgttgg gttatcctgc
     1021 atctcttctg atatttgctt ccaaacacca aagcaaagat atgcgcccta ttcttaccgt
     1081 acattctact ggcaatgtta atgaagcaaa attcgggggg gctccaaaaa cgctttcata
     1141 tgctgcgccc caggcggtca ggtcgctgct acgctcacta aaaatgctag ctgaaaatga
     1201 agagtatgaa gttactcttg aatgcacgca ccatggccca agcgacctgg atattccatc
     1261 tgtttttatt gaagtaggaa gcaacgaaac ccaatggctt gatgacgtgg caggacgaat
     1321 agttgcagaa gccatactgc ttttaaaaga tagtgattca cccgttcctt cgcatgccct
     1381 ggatgagctg gatgagatat tgataagcca ggctttcttg agaagtggag ctgattttgc
     1441 atattgcatg cagcttcgaa aaagggtcaa ggagatatgc ccttccggaa aaactgtgat
     1501 cacagaaggg attaaatgtg cgctttcctc ctgccagaca tgcatatgcc ctggagtgaa
     1561 aatagcaagg atcagtccgg gtcttttatc ggaagcggaa aaacttgata agaacggcct
     1621 taaggtattt ctctctgacc ataatatagc atatatcgaa tatgaagacg gaagatttgc
     1681 tcatatactt atcgggcttg atgattcatg tgcaaggctg gcagcggaag atttaagaga
     1741 taaatgcgtg gaaattatca aaaaacatta tgatgtctgc ctggataaag gaatcctgca
     1801 aatttcggag agaaagttca gtcccaaact ggcaaaatcc ttcgggatag aggatcttgc
     1861 attttatgga aagctggcaa gaggtgaatc cgtgataata gacggaaaaa ctataaatcc
     1921 agagatggta tatgaaacaa ataaaagagc cattaaattg aactgataaa cgatggagaa
     1981 gaagcatgga atcaataatt aaagaggcaa tttcacgagt aggcgaatct ccaatgccaa
     2041 taattgggga tacggacagg gaacttctgg aaatattaca ggaattaaag acaaatattg
     2101 ttgttatagg gtgcggaggc tccggatcaa acacaatcca gaggatatac aatgagggaa
     2161 taatcggggc tgagctgtac gcactcaata cggatgccca gcatctgtta aatattaagg
     2221 taaagaagaa aatccttatc ggtagaaata gaaccaaagg gcttggcgcg ggaagtcttc
     2281 cggctatagg acaggatgcg gccaatgaat caaaacctgc gatagagaaa gccgtaggaa
     2341 atgcagatct tgtttttata acctgtggac ttggcggtgg aacaggtacc ggctcagcgc
     2401 ctattgttgc agaaattgcg cgtgatgcag gagcattgac aatagccgtt gtaacacttc
     2461 ccttctctgt cgaaggacaa attcgaatgg ataacgctga agcaggactt gaacggctgc
     2521 gtgatgccgt ggatacggta atagttgtcc cgaatgacaa attgcttgaa gttgtacctc
     2581 accttccttt gcagtctgca tttaaggtgt gcgatgaagt cctgatgcgc tccgtcaagg
     2641 gaataacgga attgatcaca aaaccaggcc ttgtaaatct tgattttgct gatgtcaggg
     2701 tcattatgca aaagagcggc gtggccatga taggtcttgg agaagctgaa ggagagaata
     2761 aagccatcga atccgtacaa aaagctctgc gaagccc
//
LOCUS       DATGBN010000456         5309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_463430, whole genome shotgun sequence.
ACCESSION   DATGBN010000456 DATGBN010000000
VERSION     DATGBN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463430"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VKL21_11625"
     CDS             complement(<1..516)
                     /locus_tag="VKL21_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMB46458.1"
                     /translation="MEKEESKEINKTHNGLTVKIDHKKDDLGKYEEALASYDQTLEID
                     SDDEDTWGNKGTLLSKIGKYEEALASYDKALEINSNDEGTWGNKGILLGKVGKYEEAL
                     ASYDKALEINPNDENIWYNKGNVLDDLGMYEEALASYDKALEINPNDEDIWYNKGNVL
                     DKVGKYEEALAS"
     gene            complement(1045..2289)
                     /locus_tag="VKL21_11630"
     CDS             complement(1045..2289)
                     /locus_tag="VKL21_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMB46459.1"
                     /translation="MKAVIIGAGLGGLLAGAKLTKAGYDVEIFERLPFIGGRFTNLPY
                     KGFKLSTGALHMIPHGSRGPLAQMLKEVGANVTIIDSKPMAVIRKESNEIIAFHEFRN
                     ELSLTEQVKLGTVLAYSLKFKPKSDLSFRNWVLKYFDDEFLFKLADSFCGWALSLKAK
                     DVPAREMLEIIDNMYLYKGSGIPLGGCGAVTDALSGIIRSNGGRIHTQSCVERIIIDN
                     DMAKGVIVKGKTIDSGLVISDIGHFETSRLYECIDLEYLNKISNVKPSKGIKICLSSD
                     EPLIGHCGVFFTPYAQRINGINEVTNADPSLAPPGKHLVMSHQALISDDLKAEIDLGL
                     LDMAQLFPGKKYEVLLIQSYSNGWPVNRASSGSDNGNSTPFKNLYIVGDGAKGKGGIE
                     VEGVALGVRNAMVEIGISQDRR"
     gene            complement(2286..2447)
                     /locus_tag="VKL21_11635"
     CDS             complement(2286..2447)
                     /locus_tag="VKL21_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMB46460.1"
                     /translation="MKVSGECVGCGQCAAYCAFDAIIIFGRASMNENCTECGKCVKYC
                     PISAISEVK"
     gene            complement(2444..4558)
                     /locus_tag="VKL21_11640"
     CDS             complement(2444..4558)
                     /locus_tag="VKL21_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMB46461.1"
                     /translation="MTTECNDCKGTGTIIITEKECSDCKGAGKPRSISLDRLSEKDLG
                     ALMGGGLKCAKCSGTGKVSVTEPCKACGGRGKFFTCTACGIEITGKGELCENCARKET
                     VYILSPECDTRELDIGKIYEGKVQGHANFGVFIDLNPALRGLIHSSNLSHIPELGEKI
                     FVEVKNIASNGNIELLLRSPKEYQIIQVEKQLPRRKTIELTKYLGKLIHMSGEVIQIK
                     QTGGPTIFSISDEDGTVQCAAFEKAGERAYPEIKSETIVKVIGEPSMRNGALQVGIRS
                     IRQLWGQDAIGIKEQIESAIDRRAEPHEIQFLVKSDILEKLRSRMKNAAKLIRKAAYK
                     SRPIIIRHHADADGITSAIAIERALLPIIREVGGSDADRHFFRRSPSKAPFYEIEDVT
                     KDITFALEDQERFSQKMPLVVLMDNGATEEDMPAMKQAAIYGLDIMVIDHHHPDGTID
                     SMVLEHVNPAYAGGDYGITTGMIGTEIARMINPDVTEEIKHLPAVSAMGDRSSAIEGD
                     AYKALVSDKYKLEDLKKMALAIDYEAFWQRFNDGRGLMNDILNLGSNVRHQKIVDLLN
                     EQANAAITEQLEASMPNVKTQKLPNGTIMNVIDVENFAHKFTFPPPGKTSGEIHDRLC
                     TRHAGQPLVTIGYGPDFAVLRSKGVKMNIPQMVKELHSEIKGAGVSGGGHLVVGSIKF
                     VEGMRKEVLAKLAEKIGMAGIG"
     gene            4733..5002
                     /gene="albA"
                     /locus_tag="VKL21_11645"
     CDS             4733..5002
                     /gene="albA"
                     /locus_tag="VKL21_11645"
                     /inference="COORDINATES: protein motif:HMM:NF003088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HMB46462.1"
                     /translation="MTENNVVFIGNKPVMNYVLAIVTQFNNGAPEISVKARGKAISRA
                     VDAVEVSRNRFLPGTKIKEIKIGTEKIATDRGDSNVSTIEIVLSK"
     gene            complement(5036..>5309)
                     /locus_tag="VKL21_11650"
     CDS             complement(5036..>5309)
                     /locus_tag="VKL21_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HMB46463.1"
                     /translation="PIDKLVELRSQHEQGNKDFGLNVYTGNTEDMWKNGVIEPLRVKT
                     QAINSATETAIMILRIDDVLAASGGGEACSPGGAQCGMGGMPPGMM"
BASE COUNT         1401 a         1267 c         1001 g         1640 t
ORIGIN      
        1 cgatgctaga gcttcttcat acttcccaac tttatccaga acattgccct tattatacca
       61 tatatcttca tcattcggat ttatttcgag agccttatca tacgatgcta aagcttcttc
      121 gtacatccca aggtcatcca gaacattgcc cttattatac catatatttt catcattcgg
      181 atttatttcg agcgccttat cataggatgc tagagcttct tcgtacttcc caactttacc
      241 tagaagaatg cccttattac cccatgtgcc ttcatcattc gaatttattt caagcgcctt
      301 gtcataggat gccagagctt cttcgtactt cccaatttta cttagaagag tgcccttatt
      361 accccatgta tcttcatcat ccgaatctat ttcaagcgtc tggtcatagg acgctagagc
      421 ttcttcgtac ttcccaaggt catctttttt gtgatctatt ttgacggtta ggccattgtg
      481 tgttttattt atctcctttg attcttcttt ttccatatag taacctccac gaagtaattt
      541 atgtaagtat gcatagatat acttgatatc cccctcccca tgatggataa taaaataaac
      601 aatattctac cttatttttg aataatcaag aaaatctata cttgccatat aaaagtacat
      661 tttagaaaat tctaaaacaa aaattaaagt aaaataaaac cgaaaaattt attttggagg
      721 aagtatatta cttcatctgg tataaaataa ggaaccgcca ttgattttaa atttaagcga
      781 tcccaaatgg aaattgtctg gcaatattat taaaattttc gacacgagaa aaacaagaaa
      841 gattatttca agatgtggaa taaaaccact tggaaaggac tcaaaaatcg caagtgatta
      901 aggcaattct ttatgataga caaaattcca gaggcaaatg aaatctatag attcctcagc
      961 gagtttgatc tggatcaatt tgttgaaatg acattgaaga tattgaattc aatagccgta
     1021 tgtttcccat aaagattgag ttcgttatct ccgatcctga cttatcccga tctcaaccat
     1081 tgcattcctg actcccagcg ccacaccctc tacttcaatg cctccctttc ctttggcgcc
     1141 atcacccaca atataaagat tcttaaaagg ggtactgttc ccgttatcgc tgcctgatga
     1201 tgccctgtta acaggccatc cgttggaata ggattgaatt aaaagcacct catatttctt
     1261 accagggaaa agctgcgcca tatcaagaag tccaagatcg atctccgcct tcaggtcatc
     1321 cgaaatgagc gcctgatgcg acatcacaag atgttttccg ggtggcgcaa gggaagggtc
     1381 tgcattcgta acttcattga tgccattgat cctttgcgca tatggcgtaa aaaacacccc
     1441 gcagtgacct attaacggtt catccgagga aagacatatt ttaattcctt ttgaaggttt
     1501 gacatttgat atcttattta aatattccaa atctatgcac tcatataacc tgcttgtttc
     1561 aaaatgccct atatcactga tcacaagtcc tgagtcaatt gtttttccct tgacaataac
     1621 tccttttgcc atatcgttat cgataataat tctttcaaca catgattgtg tatgtatcct
     1681 tccgccattt gatcttatta tcccggaaag tgcatccgtc actgcgccgc atcctcccag
     1741 aggaattcct gaacccttgt agagatacat attatcgatt atttcaagca tttcccgcgc
     1801 cgggacatcc tttgccttaa gactcaacgc ccagccacaa aaagaatcgg caagcttaaa
     1861 gaggaactcg tcgtcaaaat attttaatac ccagtttctg aatgagagat cgcttttggg
     1921 tttgaatttc agggaatatg caagtacagt tcccagtttc acctgttctg taagtgatag
     1981 ttcatttcga aattcatgaa aagctatgat ctcgttgctt tctttcctga tcacagccat
     2041 cggtttcgaa tcaatgatgg tcacattggc gcccacttct tttaacatct gcgcaagcgg
     2101 cccgcgtgaa ccgtggggta tcatgtgaag agcgcctgtg ctgagtttga aacctttata
     2161 aggaagattg gtgaacctcc ctccgatgaa aggtaggcgt tcgaaaatct ctacatcata
     2221 tccggctttt gtgagttttg cacctgcgag caacccgcca agacccgctc caataattac
     2281 tgccttcatt ttacctccga aatggcagat atcgggcaat acttgacaca tttaccgcat
     2341 tctgtgcagt tttcattcat ggaggcacgg ccaaaaataa ttatagcatc aaatgcacaa
     2401 tatgcagcgc actggccgca tccaacgcat tccccactga ctttcatccg atcccggcca
     2461 tccctatctt ctctgcaagt ttggcaagca cctcttttct catcccttcc acgaatttaa
     2521 tgcttcctac tacaagatga cctccgccgc tcacgcctgc gccttttatc tcgctgtgca
     2581 gttctttcac catctgcggg atgttcatct ttaccccttt tgagcgcagc actgcaaaat
     2641 caggcccgta tccgatggta acaagcggct gtcctgcatg ccgggtacat aatctgtcat
     2701 gtatctcccc gcttgtcttt cccggtggcg ggaaggtaaa tttgtgggca aaattttcta
     2761 catctatcac attcattatt gttccgttgg gcaatttctg cgttttaaca ttgggcattg
     2821 atgcctccag ctgttcggtt atcgcagcat ttgcctgctc gttcaacagg tcaactatct
     2881 tctgatgtct cacatttgaa ccaaggttaa gtatatcatt cattaatccc cgcccgtcat
     2941 taaacctctg ccagaatgcc tcataatcga tggcaagcgc cattttcttc agatcctcaa
     3001 gcttgtattt atctgacaca agagctttat aagcatcacc ttcgatggca gaggacctgt
     3061 cgcccatggc tgaaaccgcc ggaagatgtt ttatctcttc ggtcacatca gggtttatca
     3121 ttcgtgctat ctccgtgcct atcattcctg ttgttattcc ataatctccg cctgcataag
     3181 caggatttac atgttcaagc accatggaat cgatagtccc atcagggtga tggtgatcaa
     3241 tgaccatgat atccagccca taaatggctg cctgtttcat cgcgggcata tcttcctccg
     3301 tcgccccgtt gtccatcaat accacaagcg gcatcttctg ggagaaacgc tcctggtctt
     3361 caagcgcaaa agtaatgtct tttgttacat cctctatttc gtagaatgga gctttggatg
     3421 gagagcgtct gaaaaagtgc cggtcagcat cagatccccc tacttcgcgg attataggta
     3481 aaagtgcgcg ctcgattgcg atcgcagacg tgatcccgtc cgcatccgca tgatgcctta
     3541 tgattatggg ccgggatttg tacgcagcct tcctgataag tttggcagca tttttcattc
     3601 ttgatcttaa cttctcaagt atgtcgcttt ttacaaggaa ctgtatttca tggggttctg
     3661 cgcgcctgtc aatagcggat tcgatctgct cttttattcc aatggcatcc tggccccaga
     3721 gctgtctgat ggaacggatc ccaacctgca atgcgccgtt tctcatcgaa ggctcaccaa
     3781 taacttttac gatagtctcg gattttatct caggataagc ccgctctccc gctttttcaa
     3841 aagctgcaca ctggaccgtc ccatcctcat ctgaaatgga aaatatcgtg ggacctcctg
     3901 tctgttttat ctggataacc tcaccagaca tatgaataag tttcccgagg tactttgtga
     3961 gttctatggt tttccttctt ggaagctgtt tttcgacctg gataatctgg tattcttttg
     4021 gacttcgcag gagcagttcg atattaccgt ttgaagcaat gttttttact tcaacgaata
     4081 tcttttctcc aagttccgga atatgactca aattgcttga atggatcagt ccccgtagag
     4141 caggattaag gtcaatgaaa acaccaaaat tagcatgacc ctgcaccttt ccctcgtata
     4201 tctttccgat gtcgagctca cgtgtatcgc attcaggact gaggatatat acggtttctt
     4261 ttctagcgca gttttcacaa agctctccct tgccggtaat ttctattccg caggctgtac
     4321 aggtgaagaa ttttcctctt cctccgcaag ctttgcatgg ttcggttaca gaaactttgc
     4381 cggtgccgct gcattttgcg cactttaatc caccgcccat aagcgcgccc aggtcttttt
     4441 cagaaagtcg gtcaaggctt atggatcttg gtttacccgc gcctttgcag tcagaacatt
     4501 ctttttcagt aattattatt gtcccggtgc ctttacagtc gttgcattca gtagtcatag
     4561 atttaagtga tagatgtgtt tcggggtaaa ataggttacg agggaattac ctttaataaa
     4621 atattccaca tctaaaaata tattcgatat ctttcaaacc tctcgaaaaa atttcggaaa
     4681 atatatgtag gtgcatcgta ttaaatagct taaaaaatcg ggagacaaca caatgacaga
     4741 gaataatgta gtatttattg ggaacaaacc agtgatgaat tatgtccttg caatagttac
     4801 acaatttaat aatggcgctc cagaaatttc tgtaaaagca agaggaaaag caatatcaag
     4861 ggcagttgat gccgtggaag tttcaaggaa caggtttttg ccgggaacaa aaataaagga
     4921 aataaagatc ggaactgaaa aaattgccac tgaccgggga gattccaatg tttccactat
     4981 agaaattgtc ctgtcaaaat agctactttt tgattttcaa gttttttgcg tacccttaca
     5041 tcatgccggg gggcattccg cccattccac attgtgcgcc gcccggactg catgcttccc
     5101 cgccgccgga tgcagcaagc acatcatcga tccgcagaat cataatggca gtttctgttg
     5161 ctgagtttat agcctgtgtc tttacacgga gaggttcgat aaccccgttc ttccacatat
     5221 cttcggtgtt tcctgtatat acattcagcc caaaatcctt attgccctgc tcatgctggc
     5281 ttctaagctc cacaagttta tcaatggga
//
LOCUS       DATGBN010000457         6094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_541031, whole genome shotgun sequence.
ACCESSION   DATGBN010000457 DATGBN010000000
VERSION     DATGBN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541031"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1478
                     /gene="pgsB"
                     /locus_tag="VKL21_11655"
     CDS             18..1478
                     /gene="pgsB"
                     /locus_tag="VKL21_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HMB46464.1"
                     /translation="MGKIAIQEKSEKSFAEVRTLYNILISFGKLQFDQFQIINDYIIL
                     LISTFFVITLYQFGINWEGIKPILGTFSFIFILIVLFVFAWIFGRREQNQHLKNINHI
                     PIRIHVNGIRGKSTVTRLIGGILREADLKTLTKTTGKMARIIDLDGSETPINRKAKPN
                     IREQIDIINLSVEKRIDALVMECMAVQPELQRVSEVKMIRSTIGVITNVRTDHLDVMG
                     PTLEDVAKSLCNSIPKNGIVVTAESKFLHIIQEHARKVKTKVIKVIPEKIDDELLISF
                     SYLNFKENIAIALEVSRLLNIPDDVAIRGMLKTEPDPGLMRIYQPKINGKVFTFVNAF
                     GVNDKDSTTMVYNELERRGHFDHKSVIGVFHARSDRVTRTIEFGNAMVSEMSFKKIIV
                     IGKMTKLFLREALKAKYPEENIIDLGESTANDAVTKMSEISKDITTVFFGCGNMVGDI
                     PLNILEIISEGCKYQEPEIHFRPENIKNHILNVNIS"
     gene            1475..1936
                     /gene="pgsC"
                     /locus_tag="VKL21_11660"
     CDS             1475..1936
                     /gene="pgsC"
                     /locus_tag="VKL21_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HMB46465.1"
                     /translation="MIILDEQSTKIAIIVGIVVSMFWYEKKNLSPGGVIVPGVTALFL
                     LTDPLIVVITLMIAFITAFVVRNMSNYIILFGRRRFSMVILVSFGIIWTVDRLTYSIM
                     PPTEIHVLGYIIPGLVANEMERQGSLKTLYSLFTITMLTLLLLLPVIGWSW"
     gene            1969..2967
                     /locus_tag="VKL21_11665"
     CDS             1969..2967
                     /locus_tag="VKL21_11665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HMB46466.1"
                     /translation="MKIDYKKSLLIIGFALFLIIATSVSGFSNVTKNENEKKYSEIDA
                     NMSGYVSILFAGDIMLGRNTENDAFEYVDLLTNSTDISFGNLEYAITNSNIRDDITKL
                     SLKSSCSATREISEAGLDIVSLANNHIMDFGERGLNDTIDCIKSLNLSYVGAGKNSYE
                     AKEMKIITVKGLKIGFLAYTARLYPSLNNSAKSNRSGVAYTSKNINHEIMNAKKKVDF
                     LIVSTHWGKEYGLSPSEFQMDENREIIDNGADLVIGTHPHVIQPIERYKDKYIFYSLG
                     NFVFDQRRNNTRDSNVFRVFINNESKIVNIERIDVRIEYTRPFIINKSNIEGNSLY"
     gene            3009..4226
                     /locus_tag="VKL21_11670"
     CDS             3009..4226
                     /locus_tag="VKL21_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46467.1"
                     /translation="MTKITIMILVVLILLGIFLIRAIDNDPVQDTTKINLKLIDCHSN
                     SHDDPEVCNLLETNDLHKQEHITLPTIVRRSLGLRDPVNGRINQQVRVGIDANGNNQI
                     DETEEYSVYTVVKDYDRTKAAYMGKGGLNRIEASSGMNVILDTRIITSTSCPSIETPG
                     QYECFFNPDPNQRYLIAIAPHGGSNEIGTAQQVEVMKQYIVEKTNIPLTTYELLGNNK
                     GGMGSHEIYHITSTEIDNTGLIHNYSHVIYPMLSSIPRDYRFSVSFHGFSPDEDEKCS
                     HPDPGANYTPDKQDGHAVVIYLGGRDTNTHKNMLRDKIESRVYEVYNNMNYSTKDSYC
                     KPLVKIPDVNSDEEKLYGGNDPDNIVNWLGSSGIQIEQSTYALYLKNTDVKSPFRDSI
                     AQAVAEYYADMYP"
     gene            4331..>6094
                     /locus_tag="VKL21_11675"
     CDS             4331..>6094
                     /locus_tag="VKL21_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HMB46468.1"
                     /translation="MVSIIIIIKSITKNFLSVLSTKNFRDLFLISFLLLFTFPILASC
                     ALAYTDLLLNPGFESGTTTPLNWTLVNQGGNTPIWDSVSHSGSRSIKISIPGTTNIIS
                     GYPQSDLIIAQPLAIYAVSMWGKTQNAGGTNTPAARVVELDANKNWIRQTNLPVFSRG
                     TNDWTQKTVEFQIGSNTKYLYVYANIWKGYGNFWIDDVELSLKNTPTPAPTPAPTPVR
                     KSTKISLKVVDCYSNSHEDPEVCNLLGANKLHTQEHITLPAIVRRNFGLRDPVDGRIN
                     QQIRVGVDANGNNRIDETEAYAVYTVVMDYDRTQTAYMGTKGLNRIGASSGMKVIMDT
                     QVLTSPTCPSIGTIGQYECFFNPNPNQRYLIAIAPHGGSNEIGTAQQVDALKQFLVDK
                     TNIPITTYQLLGTKKGGNSHDSYHITSTEIDNTNLIHDYFHVSYPLLSKIPRDYQFSV
                     AFHGFSLDEDEKCPSPDPGANYTPDKQDGHAVVIYIGGRDIDAHKQILRNKIESNVYA
                     VYNNMSSSNKKSYCKPLVKIPDVNSDQGKLYGGDNPENIVNWLSGSGLQFEESSYARY
                     IKNTNPIVKSPFRDSIARAVAE"
BASE COUNT         2072 a         1083 c         1254 g         1685 t
ORIGIN      
        1 tatagctggg gctttctatg gggaaaatag caatacagga aaaatcagaa aaatcatttg
       61 ctgaagtccg gacattatat aatatattaa taagttttgg gaaattacaa tttgatcaat
      121 ttcagataat aaatgattat ataattcttt taatctccac attttttgtt ataacattgt
      181 atcaattcgg tattaactgg gaaggtatta aaccaatatt aggaacgttc agtttcatat
      241 ttattttgat agtgcttttc gtattcgcct ggattttcgg gaggcgggag caaaaccaac
      301 atttaaaaaa cattaaccat attcccatcc gaattcacgt caatggcata cggggaaagt
      361 ctacggtcac aaggttaata ggaggtattc tcagggaagc ggatttgaaa actttgacaa
      421 agacaactgg caagatggcg cgaatcatag atttggacgg aagcgaaacg cctattaata
      481 gaaaagcgaa accaaacata agggagcaga tagatataat taacctatct gttgaaaaga
      541 gaattgatgc gcttgttatg gaatgcatgg cagttcaacc tgaactccag agggtgtcag
      601 aggtaaagat gatacgatca acgataggtg taataacaaa cgtgcgcaca gaccatctgg
      661 atgtaatggg tccaaccctg gaagatgttg caaagtcctt atgcaattca attcccaaaa
      721 atgggatagt ggttacagca gagagcaaat tcttacatat aatacaggaa catgccagga
      781 aagtgaaaac taaagtaata aaagtaattc cggaaaagat cgatgatgaa ttattaatat
      841 cttttagtta tttgaatttc aaagagaata tcgctattgc ccttgaggtt tcccgacttc
      901 taaatatacc tgatgatgtg gcaataagag gaatgcttaa aacagaaccc gaccccgggt
      961 tgatgaggat atatcaacca aaaataaatg gaaaagtatt cacctttgtg aatgcattcg
     1021 gcgtaaatga taaggacagc accacaatgg tgtataatga acttgaaagg agagggcact
     1081 ttgaccacaa gtctgtaatt ggggtattcc atgcaagaag tgacagggtt acaagaacca
     1141 tagagttcgg aaatgccatg gtatctgaga tgagtttcaa gaagatcata gttattggaa
     1201 agatgaccaa actttttttg agggaagccc ttaaagcaaa gtaccctgag gaaaacataa
     1261 tagacctggg ggaaagtact gcaaatgatg ctgtaactaa gatgagcgaa atatctaaag
     1321 atattactac tgtgttcttt ggatgtggaa atatggtcgg tgatataccc ctgaatatac
     1381 tggaaatcat atctgaagga tgcaaatatc aggaaccaga aatacacttc cgcccggaaa
     1441 atattaaaaa tcatattttg aatgtgaata tatcatgata attcttgatg aacaatccac
     1501 aaaaatagca attatcgttg gaattgttgt tagcatgttc tggtatgaaa aaaagaacct
     1561 ttccccagga ggggttattg tgccgggggt tactgcgctt ttcttgttaa ccgatccttt
     1621 gatcgtagtg atcacattga tgatcgcttt tataactgcc tttgtggtaa gaaatatgtc
     1681 aaattacata atactcttcg gaaggagaag gttctctatg gtcatattag tcagttttgg
     1741 cataatatgg actgtagata ggcttacata ttcaataatg ccaccaaccg aaattcatgt
     1801 tcttggatat atcattcccg gcctcgtcgc aaacgaaatg gaaaggcagg gctcgttaaa
     1861 aacattgtat tcactcttta ctattacaat gctcacgctt cttctgcttc ttccggtcat
     1921 cgggtggtcg tggtgaatgg ataacttgta tcatgagggt cagggggaat gaaaatagat
     1981 tataaaaaat ctcttctgat tataggattc gccctgtttc tcataatagc aacatctgtg
     2041 tctggatttt cgaatgtgac aaaaaatgaa aatgaaaaga aatattctga aatagatgca
     2101 aatatgtcag gttatgtcag tattctgttt gcaggagata ttatgcttgg aaggaatacc
     2161 gaaaatgatg ctttcgaata tgtggattta ttaacgaatt ctacggacat ctcatttggc
     2221 aatttggaat atgccatcac aaatagcaat attcgagatg atattaccaa attaagttta
     2281 aagtcaagct gttctgcaac acgagaaata agtgaagcag gattagatat cgtatcttta
     2341 gcaaacaatc atattatgga ttttggcgaa agagggctaa atgatacgat cgattgtatc
     2401 aaaagtctca atttaagtta cgtaggagca ggaaagaact catatgaagc taaagaaatg
     2461 aaaattatca ctgtgaaagg tttgaagata ggtttcttag catatacagc cagattatat
     2521 ccatcgttaa acaactcagc aaaaagtaac agatcaggtg tggcttatac atccaaaaat
     2581 attaaccatg agatcatgaa tgctaagaaa aaagtagatt tcttgatcgt cagtactcat
     2641 tggggaaaag agtatggttt atctccttca gaattccaaa tggacgaaaa cagggaaatt
     2701 attgacaatg gagcagactt agtaatagga acacatccac atgtaattca gccaatagaa
     2761 agatataaag ataaatatat tttttatagt ctgggcaatt ttgtttttga ccagagacgc
     2821 aacaatacaa gagattctaa tgtctttaga gtctttatta ataacgaatc aaaaatagta
     2881 aacattgaga gaatagatgt aaggattgaa tataccaggc cattcattat aaataaaagt
     2941 aacattgaag gaaactcatt atattaaatt taagattcct gcatggagga atgttgcagg
     3001 gctgttatat gactaaaata acaataatga ttttggtagt tttgattttg cttggtattt
     3061 ttttaatacg agctattgat aatgatccag tccaggatac tacgaaaatc aacctaaaac
     3121 tgatcgattg ccattcaaat tcacacgatg atccggaagt atgtaattta ttggagacaa
     3181 atgatcttca taaacaagag cacataaccc tgcccactat tgttcgcagg agtctcggtt
     3241 tacgcgatcc tgtaaacgga aggatcaatc aacaggtccg ggtcgggata gatgcgaatg
     3301 gaaataatca aatagatgaa actgaggaat actctgtcta tacggtcgtg aaagattatg
     3361 acaggacaaa agccgcatat atggggaaag gtggattaaa tcgcattgaa gcatcaagcg
     3421 gaatgaacgt cattctggat accaggatca ttacttcaac atcgtgtcca tccatagaaa
     3481 ccccgggaca atatgagtgc tttttcaatc ccgatccaaa ccagagatac ttgatagcaa
     3541 ttgcacctca tggcggatcg aacgagattg gaactgccca acaggtggag gtaatgaaac
     3601 aatacatagt cgaaaaaacc aatatacctc taacgacgta tgaattgttg ggaaataata
     3661 aaggcggtat gggttcgcac gagatatatc acattacctc gacggaaata gacaatacag
     3721 gtttaattca taattactct catgtgatct atccgatgct tagtagtatc ccacgcgatt
     3781 accggttctc agtgtcattt catggattct ctcctgatga agatgaaaaa tgttctcatc
     3841 ctgatccagg cgcaaattat actccggata aacaagatgg gcatgccgtt gtcatctacc
     3901 tgggaggacg agataccaat acccacaaaa atatgttaag agataagata gaatcgaggg
     3961 tttatgaagt ttataataat atgaattatt caactaaaga cagttattgc aaaccactgg
     4021 tgaaaattcc tgatgtaaat tcagatgagg agaaattata cggggggaat gacccggata
     4081 atatcgtgaa ctggcttggc agctcgggta ttcagatcga acagagcact tatgcactat
     4141 atcttaaaaa taccgatgta aaatccccct tcagggattc tatcgcccag gccgttgccg
     4201 aatactatgc tgatatgtac ccctgattgt tcattgcaga gtattatttt tccgctgttt
     4261 tttcagattc tgtcctgctc atgatatttg gtcataagag ttatcctatg gccataatta
     4321 tattctatgt atggttagca ttattataat cataaaatca ataacaaaaa atttcctgtc
     4381 cgtgttaagt acaaaaaatt tcagggattt atttcttatt tcctttttgt tactatttac
     4441 tttccctatt ttagcatctt gcgcccttgc atatactgac cttctattaa acccgggttt
     4501 tgaaagtgga acaacaactc ccctaaactg gacacttgtg aatcaaggcg gaaacacacc
     4561 tatctgggat agcgtatccc acagcggatc acgatcaata aaaataagca tcccaggaac
     4621 aacgaatatt atatccggat atccacaatc tgacctaata attgcacagc cccttgcgat
     4681 atatgcagtc tcaatgtggg gaaaaacaca aaacgcaggt ggaacaaata ccccggctgc
     4741 cagggtagta gagcttgatg caaacaagaa ttggatacgc cagactaacc tccccgtatt
     4801 cagtcgaggc acaaatgact ggacacaaaa aacagtagaa ttccagatag gttcgaatac
     4861 aaaatatctt tacgtctatg ccaatatctg gaaaggctat gggaatttct ggattgatga
     4921 tgtagagtta agcttgaaaa acacccccac accagcaccc actccagcgc caactcctgt
     4981 ccggaagtct acgaaaatca gcttgaaagt agtggattgc tattcaaatt cacacgaaga
     5041 tcctgaagta tgcaatttgt taggggcaaa caagctccat acacaagaac acataactct
     5101 gcccgctatt gtgcgcagga acttcggatt acgtgatccg gttgatggaa ggatcaatca
     5161 gcagattcgg gtcggggtag atgcgaatgg aaacaatcga atcgatgaaa ctgaagcgta
     5221 cgcagtgtat acggtcgtga tggattatga taggacacag accgcttata tgggaactaa
     5281 gggactaaat cgcattggag cttcgagtgg aatgaaagtt atcatggaca ctcaagtcct
     5341 tacttcacca acatgtcctt ccataggtac gataggacaa tatgaatgct ttttcaaccc
     5401 caatccgaac cagagatatt tgatcgcaat tgcaccccac gggggatcga acgagatcgg
     5461 aactgctcaa caggtggacg cattgaaaca attcctggtc gataaaacca atatacctat
     5521 cacgacatat caattgttag ggacgaaaaa aggcggcaat tcgcacgata gctatcacat
     5581 tacatccaca gaaatagata atacgaattt aattcatgat tacttccacg tgagttatcc
     5641 attgcttagc aagatcccac gcgattacca gttctcagtg gcatttcatg gattctctct
     5701 tgatgaagat gaaaaatgtc cttctcctga tccgggggca aattatacgc ctgataaaca
     5761 agatgggcat gccgtggtca tatacatagg gggacgagat atcgatgccc ataaacagat
     5821 attacgaaat aagatcgaat caaatgttta tgcggtttat aataatatga gttcttcaaa
     5881 taaaaaaagt tattgcaaac cactcgtgaa aataccagat gtaaattccg atcaaggtaa
     5941 gttatatggg ggcgataatc cggaaaatat tgtgaattgg ctcagtggtt cgggtctcca
     6001 gtttgaagag agtagttatg caagatatat taaaaatacc aatcccattg tgaaatcccc
     6061 tttcagagat tcaatcgccc gggcagttgc cgaa
//
LOCUS       DATGBN010000458         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_153128, whole genome shotgun sequence.
ACCESSION   DATGBN010000458 DATGBN010000000
VERSION     DATGBN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153128"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..332
                     /locus_tag="VKL21_11680"
     tRNA            join(243..281,297..332)
                     /locus_tag="VKL21_11680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Arg,seq:cct)
     gene            complement(340..1089)
                     /locus_tag="VKL21_11685"
     CDS             complement(340..1089)
                     /locus_tag="VKL21_11685"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HMB46469.1"
                     /translation="MIPKSHPRYESLMTRELIVEGIKQGITSIQGLIAQGRGEAFDYL
                     IGEKSTEYALLAEKVAAAKLLLARKPVISVNGNVAALVPGEIIRLGELVNAPLEVNLF
                     HRTQERVNKIIVHLRAMGAKNMFTKSDALIPGLGHERAKVDNNGIFIADAVLVPLEDG
                     DRCKALVDMGKTVIAIDLNPLSRTAQYATVTIVDNITRAVPNISKFAEVLKDLDHEEL
                     REMVRGYDNKQTLSLAIDEIKKNLESFSGRF"
     gene            complement(1086..1931)
                     /locus_tag="VKL21_11690"
     CDS             complement(1086..1931)
                     /locus_tag="VKL21_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate kinase"
                     /protein_id="HMB46470.1"
                     /translation="MQHKIVKAFAPAHISGIFIIDIKNDPSLSGSMGCGICLEDGAVT
                     QVLPAKETIIRINGAVTQAPTTLSTIGLLTAEPVLVDTTFKTPIGAGFGASGAGALSA
                     ALALNECLSMNLTLKDLARAAHCAEVTNRTGLGDVTGMIFGGVVVRKKAGAPFLGIID
                     KIPCRNTIISWISFNEISTKSVLTDDLKKKSINKSGKARLKELLKKPTIENFMIQSAA
                     FAKDIELMSSGVRDSIEAVEAAGGLASQAMLGDTVFAINDNDALLEFGEVHRSRISNA
                     GAHLL"
     gene            complement(1970..2428)
                     /locus_tag="VKL21_11695"
     CDS             complement(1970..2428)
                     /locus_tag="VKL21_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMB46471.1"
                     /translation="MIAIDTGVWIEYINTRGLLHTEAKAVVDSVNQGKITAILTPLTL
                     TEIFYVAQRVYQEVYTQSQSEILAKKLYDFIYYHPNVEVKPLDYELCLNAGIIKTRYN
                     IALSDCFLLALSEQDNSTIVFKNLESEMKQNKQELIKRFDIIFLEDYGIP"
     gene            complement(2425..2685)
                     /locus_tag="VKL21_11700"
     CDS             complement(2425..2685)
                     /locus_tag="VKL21_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMB46472.1"
                     /translation="MTTVTISSKGQIVIPKKLRETLGLTIGKKLKLFQENKKIILVTE
                     TEIKPSELFVSASPHAVDKAMKKSRKIDEGKIRRLLNDLGIK"
     gene            complement(2731..>3653)
                     /locus_tag="VKL21_11705"
     CDS             complement(2731..>3653)
                     /locus_tag="VKL21_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMB46473.1"
                     /translation="FKNGDVVLTTDREHNSNLVPWQVQAHKRGIKHVIVHSNPDNTFD
                     LDAFSRLFDKNNIVRLVSMVHTANLDGYTIPVKEIIKIAHDHGALVMLDGAQSAPHKH
                     VDVKALDVDFFALSVHKMAGPTGMGVLYGKHHLLEELAPFIVGGDTVSDTTYEGAKFL
                     PPPDKFEAGLQNYAGIIGSGAAVDYISGVGMSNIEDHEKRLNTIITRGIKDLPGLKII
                     GPGEPDLRGGIISFTVELPKGGDAHDIALILDETENIEVRSGAFCVHSWFNYRKCEAA
                     VRASLYLYNTEEEARKFIDVLGKTIELFNP"
BASE COUNT          899 a          851 c          742 g         1161 t
ORIGIN      
        1 tagagatgaa ttggcagcga agatacttgc acttgttgaa aaatatgtgg ttgaaataac
       61 ctattattaa ctttgcgtta ttcaggtatt ggtatagccc ttatgatagc gatgtgatag
      121 taatatgaat aatatttcaa gggcccctat tcctcccctc ccttttttcg tcaggggact
      181 tctgggccac ttgaccccac aaggttaaat acaaaccact gacatttagt caaaacccta
      241 cagggcgcgt ggcctagtct ggatagggcg gcagcctcct aaataagatc ggagtcagct
      301 gtaggtcggg ggttcgaatc cccccgcgcc cgtatcaaat taaaaccttc ctgaaaatga
      361 ttccaggttc ttttttattt catcgattgc aagggagagt gtctgtttat tatcatatcc
      421 cctgaccatc tctcgcagtt cttcatggtc caggtctttt aatacttctg cgaattttga
      481 aatgttcggg acagcgcgcg taatattatc tactatcgtt accgttgcat attgtgctgt
      541 ccttgaaaga ggattcaggt caatggctat tacggttttg cccatatcca caagggcttt
      601 gcacctgtcc ccgtcttcaa gaggcacaag cacagcatca gcaataaaga tgccattgtt
      661 atctactttt gcccgttcat gcccaagtcc gggaatcaaa gcatcactct ttgtgaacat
      721 attttttgcc cccatggctc gaagatgcac gataatttta tttactcttt cctgtgtcct
      781 gtgaaataaa ttgacttcaa ggggcgcgtt tacaagttcg cctaatctta tgatttctcc
      841 gggaacaagg gctgcgacat ttccattcac tgatattaca ggttttcgcg caagaagcaa
      901 ctttgccgct gctacttttt cggccagcaa agcatattct gtgcttttct cacctatcag
      961 gtaatcaaaa gcttctcccc gcccctgtgc gatcaatccc tgaatacttg ttattccctg
     1021 ttttattccc tcaactatca actcccgcgt cattagggac tcatatctgg ggtggctttt
     1081 cgggatcata gaagatgcgc tccagcatta cttatcctgc ttctatggac ttccccgaat
     1141 tccaaaaggg cgtcgttatc gtttatggca aataccgtat ccccaagcat tgcctggctt
     1201 gcaaggccac cggcagcctc gacagcttct atagaatctc ttactcctga gctcatgagt
     1261 tcaatatctt ttgcgaatgc agccgattgg atcataaaat tttcaatcgt gggttttttt
     1321 aagagttctt tgagccttgc ttttcccgat ttatttatac tcttcttttt caggtcatcg
     1381 gttaaaactg attttgtgga tatctcattg aagcttatcc aggatattat ggtgttcctg
     1441 catgggatct tatcgataat tccaagaaaa ggcgcgcccg cttttttcct cacgaccacg
     1501 ccgccaaaga tcattcctgt aacatcacca agccctgtcc tgtttgtaac ttctgcgcaa
     1561 tgggctgccc gggctaaatc tttcaaggtc aaattcatgg acagacattc atttaacgca
     1621 agagcagcgc tcaatgcccc ggcgccgctt gctccaaacc ctgcgccgat gggtgttttg
     1681 aatgttgtat caacaagtac cggttctgcg gtcaggagtc ctattgtaga taatgtcgta
     1741 ggagcttgcg taacggcgcc attgatcctg attattgttt cttttgcggg aagtacttgt
     1801 gtcacggcgc cgtcttccag acatattccg cagcccatgg agcctgaaag tgaggggtcg
     1861 ttttttatgt cgattatgaa aatacctgag atatgggcag gcgcaaatgc tttaacgatt
     1921 ttatgttgca ttgtcactta tatttactgc ggacacaaaa aactttacat taaggaattc
     1981 catagtcttc caggaatatt atatcaaaac gtttaattaa ttcctgttta ttttgcttca
     2041 tttcactttc taaattttta aatacaatag tcgagttatc ttgttcggat agggcgagta
     2101 aaaagcaatc cgataatgca atattatatc tggtttttat gatcccggcg ttcaggcaga
     2161 gttcgtaatc caggggctta acttctacat ttggatggta atatatgaaa tcatagagtt
     2221 tttttgcaag tatttcagat tgagattgtg tatatacttc ctggtatacc ctctgtgcta
     2281 cataaaatat ttcagttaat gtcaatgggg tcaaaattgc agtgatcttt ccctggttca
     2341 ctgaatcaac cactgctttt gcttcggtat ggaggaggcc acgggtatta atatattcta
     2401 tccagacacc cgtgtctatt gcaatcattt tattcccaga tcattaagaa gtctcctgat
     2461 ttttccctca tcaatttttc gagacttttt catggcttta tcaacggcat ggggtgacgc
     2521 acttacaaat aattccgaag gctttatttc tgtttccgtt acaagtatta tcttcttgtt
     2581 ttcttgaaat aacttcagct tttttcctat tgttaaaccc agcgtttccc ttaatttttt
     2641 ggggatcact atctgccctt tgcttgaaat tgttacagtt gtcataagga aatgtaagac
     2701 gattatctta tttaagtctt actcttgttt tcatggatta aacaactcta tcgtcttccc
     2761 cagcacatca atgaacttgc gtgcctcttc ctccgtatta tacaaataga gcgaagctct
     2821 gaccgctgcc tcgcatttcc tgtaattgaa ccacgaatgc acgcagaatg cgcctgagcg
     2881 aacttctatg ttctcagtct catcaagaat aagggcaata tcatgggcat caccgccttt
     2941 tggaagctcg acagtgaacg aaatgatacc acccctgaga tccggctccc ccggacctat
     3001 gatctttaat cccggtaagt cctttattcc tctggtgata atggtattga gccgcttttc
     3061 atggtcttca atatttgaca ttccaacacc gctgatataa tccacggctg cgcctgaacc
     3121 tatgatgcct gcataattct gaaggcctgc ctcgaattta tccggagggg gcaggaattt
     3181 tgcgccttca tacgtagtgt cgctgacggt atccccccca actataaaag gtgcaagctc
     3241 ttccagtaaa tggtgtttgc cataaagcac gcccatcccg gtagggcctg ccatcttatg
     3301 cactgaaagc gcaaagaaat ccacatcaag cgcctttaca tccacatgct tgtgcggcgc
     3361 gctctgggcg ccgtcaagca tcaccagcgc gccgtggtca tgtgcgattt ttattatttc
     3421 ttttacagga atggtatagc cgtcaaggtt ggcggtgtgt accatgctca caagccgcac
     3481 aatattgttt ttgtcaaata gtctggaaaa tgcatcaaga tcgaaggtat tgtcaggatt
     3541 tgagtgaaca atgacatgct ttatccctct tttatgcgcc tgcacctgcc agggaacgag
     3601 atttgaattg tgctcccggt ctgttgtaag gacaacgtcg ccgtttttga aat
//
LOCUS       DATGBN010000459         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_347631, whole genome shotgun sequence.
ACCESSION   DATGBN010000459 DATGBN010000000
VERSION     DATGBN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347631"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VKL21_11710"
     CDS             complement(<1..245)
                     /locus_tag="VKL21_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46474.1"
                     /translation="MGKYIDVQILNLMEEHQEELYNAFTTEKKQKQANSETKQKTKEI
                     QTIQKTLGGSLVSTIPKVRTTDGHLMDWDRNTIVSQLL"
     gene            complement(330..593)
                     /locus_tag="VKL21_11715"
     CDS             complement(330..593)
                     /locus_tag="VKL21_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HMB46475.1"
                     /translation="MQGIIIYSTKNCPNCRVLKQFLENVKVQFTEVDMATPAALTELR
                     MNGVFTMAAPVLQIGNKFHTYNELFTQDRINQDKVESLVNDAI"
     gene            809..2530
                     /gene="argS"
                     /locus_tag="VKL21_11720"
     CDS             809..2530
                     /gene="argS"
                     /locus_tag="VKL21_11720"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMB46476.1"
                     /translation="MFLKFKDEVSSILSSAVEKAGFETDDLALTESPHADLASSVGFK
                     MAPKYRKSPKEISEKIYKNIAIQADSYVARAEMAGPYINFFMSRTFLNETVLRVLLDR
                     ENFGNLNNKGNIILEHTSANPDGPLHIGHIRNSVIGDTLARILKRAGYDVETQYYIND
                     MGRQIAVVVWGLENFQFDRTKKKDHAIAEVYINANKSLVDEKEHSPEVDAIMKKYEAG
                     EPEIGGKFKNAIDTCMEGIKESLARMNIHHDKFIWESQFVRTGEVTKIMDRVKALDRS
                     ELKDGAIMVDLKDMGIQKSLVIQRSDGTSLYTTRDLSYHNWKASQCDRMIDILGADHK
                     LISSQLKAVLRILGHKEPQIVIFEFVSLPEGSMSTRRGVFISADELIDEVEKAAFEVV
                     DKKLPDKDEEFKNKVAAFVSIGAVRYDIVRISPEKATVFDWKEAVDFEKQGAPFIQYA
                     HARACSIIKNARDDDSYYGAFSDRNSPPFDPNVLLEAQEIALIKKLSVFERVIDDASR
                     ELKPNLLAIYARELADSFNQFYRFVPVLTAEPAEFRAARLALVECSRIVLANALDTLG
                     IFAPDSM"
     gene            2550..3365
                     /locus_tag="VKL21_11725"
     CDS             2550..3365
                     /locus_tag="VKL21_11725"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase"
                     /protein_id="HMB46477.1"
                     /translation="MKLISIVGYKKTGKTTLVGQLVKALKKHGSVGTIKHLHEHDINA
                     KDTDTWKHAQAGADVVVAVTPNELVKFSHDNDLESALDELANAGMDFAIVEGFKDSKL
                     PKIALGDVEASEIVKRVDTFESANIDELVDIIMKQPEFHTLDSLLQKLKSSSSIEKAG
                     AIGTFTGIVRGVTDKTHTEFLEFEAHWELARKKMDEICRQLKKEEGIVDVLMHHRLGI
                     IPRGGDIVYIAVAAWHREELFPVLREAIERLKEQVPIWKKEHTVKGDWWVHDV"
     gene            complement(3379..3693)
                     /locus_tag="VKL21_11730"
     CDS             complement(3379..3693)
                     /locus_tag="VKL21_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46478.1"
                     /translation="MVTIDPCIRLKVIQSQLLPAILKSAVENTSSDIKTAIELNLPTL
                     EKKCNELAEKCQKKYPDCGNEIELCKHENIITIFAQTREKLDKIWIEREKQWKETAGS
                     DL"
     gene            3803..>4548
                     /locus_tag="VKL21_11735"
     CDS             3803..>4548
                     /locus_tag="VKL21_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HMB46479.1"
                     /translation="MIKKDVIIIGAGASGMMCAIESGKRGRNVLVLDHADKIGKKIRI
                     SGGGRCNFTNINIHPTNYISNNPHFCKSALSRFTPRDFIDLLEKHGVQYEEREEGQLF
                     CVKDSGEIIKMLEKESNDAGVNIILNCQINGVKKEESFIISTDHGIFQSESLVIATGG
                     LSYPRIGASGIGYNIAKQFGLKITGIKPALVPFIFSPKDLKIFGELSGISIDGIIKCN
                     KIEFRGSILFTHRGLSGPAILQISSYWKEG"
BASE COUNT         1339 a          913 c         1004 g         1292 t
ORIGIN      
        1 agaagctggc ttacgatcgt attcctgtcc caatccataa ggtggccgtc tgttgtcctt
       61 accttcggga tggttgatac aagggagccc ccgagtgttt tctgtatcgt ctgtatttct
      121 tttgtttttt gtttcgtttc cgaatttgcc tgtttttgct tcttttccgt agtaaaagcg
      181 ttatacagtt cttcctgatg ttcttccata agattcaata tctgtacgtc tatatatttg
      241 cccatctttt tgcacctctt tatatttcgg ccatgcagtt tttactcaag gctgcgcaga
      301 accagtaccc tgcggttctt gtcgctcccc tatatggcat catttaccag agattcaact
      361 ttatcctggt tgatcctgtc ttgcgtgaat aactcattat atgtatggaa tttattacct
      421 atttgcagca ccggcgcagc catggtgaat acgccgttca tcctcagttc tgtcaatgcc
      481 gccggggtag ccatatctac ctctgtgaat tgtaccttta cgttttccag gaattgcttc
      541 agtaccctgc aattcggaca gttttttgtt gaatatataa tgatgccctg cacgctttta
      601 atctccttgc gaaaccaatg attcatttca aagcttataa cggtttttca aactacatct
      661 ggataccaat tgtcattgtt tgttatcata tactttatca aattagatta taatggtatc
      721 ttatttgtta tcatattaag tatcagctta gatacaaaat gacttgagga aaaattatct
      781 acttagcttg tgtgtaaacc ttgattttat gttccttaaa ttcaaagatg aagtttcatc
      841 gatattatcc agcgccgttg aaaaagcagg attcgagaca gacgacctcg ctttgactga
      901 atcgcctcat gcggaccttg cgtcttctgt tggatttaaa atggcgccga aatataggaa
      961 aagccctaaa gagatcagcg aaaaaatata caaaaatatt gcgatacagg cagattctta
     1021 tgttgccagg gccgaaatgg caggccccta tatcaacttt ttcatgagcc gtactttcct
     1081 gaatgagact gtccttcgcg tgcttcttga cagagagaat ttcgggaacc tgaataataa
     1141 ggggaatatt atcctggagc atacttcagc gaaccccgat ggtcctttgc atatcggcca
     1201 tataaggaat tcggtaatag gggatactct tgcccggatc ctcaagcgcg ccggttacga
     1261 tgtggagacg caatattata taaacgatat ggggcgtcag atcgcagttg ttgtatgggg
     1321 acttgagaat ttccagttcg acaggactaa gaagaaagac catgccatcg ccgaagttta
     1381 cattaatgcc aataaatcgc tcgttgatga aaaagaacat tcaccggaag tggatgccat
     1441 aatgaaaaaa tatgaggccg gcgaaccgga aattggagga aaattcaaaa atgccatcga
     1501 tacatgcatg gaagggataa aagaatcact ggcgcgcatg aatatacatc atgataaatt
     1561 catctgggaa tcacaattcg ttcgcacagg ggaagttaca aagatcatgg atcgggtaaa
     1621 ggcacttgac cgttctgaac ttaaagatgg cgcgatcatg gttgacctga aagatatggg
     1681 gatccagaag tcattggtga tccagcgctc ggacgggact tctctttaca ccacccgcga
     1741 cctctcatac cataactgga aagcctcaca gtgcgacaga atgatagaca tactgggtgc
     1801 agaccacaag ctcatttcat cacagctaaa agcagtactc aggatcctgg gtcataaaga
     1861 accccagatc gttatttttg agttcgtatc cctgccagaa ggttctatga gcacgcggag
     1921 gggtgttttc atttcggctg atgaactgat cgatgaagtt gaaaaagcag cttttgaagt
     1981 agtggataag aaacttccgg ataaagatga ggaattcaaa aataaagtag ctgcctttgt
     2041 gagcatcgga gcagtgcggt atgatattgt caggatatcc ccggagaagg ccacggtatt
     2101 tgactggaaa gaagctgtgg actttgaaaa acagggggcg ccattcatcc agtatgctca
     2161 tgcaagagcc tgcagcatca taaagaatgc aagggatgat gattcatact atggggcttt
     2221 ctccgatcgg aatagtcccc cgtttgaccc caatgtactg cttgaggcac aggaaattgc
     2281 cctcatcaag aaattatccg tttttgaacg cgtcatcgac gacgcatcaa gggaattgaa
     2341 accaaatctt cttgccatat acgcaaggga actggcggat tctttcaacc agttctacag
     2401 gttcgtacct gttcttacgg cagagcctgc tgaattcagg gcagcgcgtc ttgctcttgt
     2461 tgaatgttcc cgcatcgttc ttgcaaacgc acttgacact ctcgggatat ttgctcctga
     2521 ttcaatgtaa tgactgcaat atcaggttta tgaaactaat ttccatcgta ggttataaaa
     2581 aaacaggaaa aaccacctta gttggacagc tggtaaaagc attaaaaaaa cacggttccg
     2641 tcgggacgat aaaacatctc catgaacatg atatcaatgc aaaggatacc gatacctgga
     2701 aacatgcgca ggctggcgct gatgttgtag ttgctgttac cccgaatgag cttgtgaaat
     2761 tttcacatga caatgacctt gaaagtgcat tggatgagct tgcaaatgca ggaatggatt
     2821 ttgcgatcgt tgaaggattc aaggacagca aattgcctaa aatagcgctt ggcgatgttg
     2881 aagcgtctga aattgtgaaa agagtggaca cctttgaaag tgcaaatatc gatgaattgg
     2941 ttgatatcat aatgaaacaa cccgaatttc atacacttga ttctttgctt caaaagctca
     3001 aaagttcaag ttcgattgaa aaagcaggag ctatcggaac atttactgga attgtcaggg
     3061 gcgtgacgga caaaacccac accgagttcc tggaatttga agcgcactgg gaacttgcca
     3121 ggaaaaagat ggatgaaata tgcaggcaac tcaagaaaga agagggcata gtcgatgtac
     3181 tgatgcatca caggctagga attatcccca gaggcggaga tattgtttat atcgctgttg
     3241 ccgcatggca cagggaagag ctttttccgg tattaaggga agcgatcgaa cgcctgaagg
     3301 agcaagttcc tatctggaag aaagagcata cggtgaaagg ggattggtgg gtgcatgatg
     3361 tctaaggatt ttaaaatatt aaagatcaga ccctgcagtt tctttccact gtttctctcg
     3421 ttcaatccag atcttatcca gcttttccct ggtctgtgca aaaatagtta taatattttc
     3481 atgcttgcat aactcaattt cgtttccaca atcgggatat tttttctggc atttttcggc
     3541 aagttcattg catttctttt caagggttgg cagattgagc tcaattgctg ttttaatatc
     3601 agaacttgta ttctctactg cgcttttaag aatagctgga agtagctgag attggatgac
     3661 cttaagtcgt atgcatggat caatggtgac caatttaaaa cctcattaac catttacaaa
     3721 tgaaatttgg actataatta ttttaccccc ttcaataagg ataatacaaa agaatgaaac
     3781 aagtacgaaa ccaagaaaat atatgatcaa aaaagatgtt atcatcatcg gagcaggtgc
     3841 atcaggcatg atgtgcgcca tcgaatcggg aaaaagaggc cgcaatgttc ttgttctgga
     3901 ccatgccgat aaaattggaa aaaaaatccg gatttccggc ggaggaagat gtaattttac
     3961 aaatatcaat attcatccca cgaattatat ctcaaataat cctcactttt gcaagtctgc
     4021 gctttcccgt tttacccccc gtgatttcat agaccttctt gaaaaacatg gcgttcaata
     4081 tgaagaaaga gaagaggggc aattattctg cgtcaaggac tccggggaaa taataaaaat
     4141 gttggagaag gaaagtaatg atgccggagt taacattatt ttaaattgcc agataaatgg
     4201 cgtgaaaaaa gaagagtcgt tcataatatc cactgatcat gggatttttc aatccgagtc
     4261 gcttgttatt gcaaccggcg gtttgtctta tccccggata ggcgcttcag gaatagggta
     4321 taacatcgca aagcagttcg gtcttaaaat cacagggata aagcctgcac tggttccttt
     4381 tattttttcc ccaaaagacc ttaaaatatt tggagaatta agcggcatct caattgacgg
     4441 cattattaaa tgcaacaaaa ttgagtttcg gggaagcatc cttttcactc acagaggact
     4501 tagcggtccc gcgatacttc agatctcctc atactggaaa gagggcga
//
LOCUS       DATGBN010000460         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_36230, whole genome shotgun sequence.
ACCESSION   DATGBN010000460 DATGBN010000000
VERSION     DATGBN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36230"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..506)
                     /locus_tag="VKL21_11740"
     CDS             complement(84..506)
                     /locus_tag="VKL21_11740"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMB46480.1"
                     /translation="MRYQFEQCLERGKIVKIEIAPDIISKEINEAKNDLDSCERSLQE
                     RNFKWAIIQAYYSMFHSFRALIFSKGYREKSHTCIKYAIESLFVDAGTLDNRLMQDFS
                     YAMKIREVADYGSVYSEDSALELRNSASKIFEVCKELI"
     gene            complement(506..1045)
                     /locus_tag="VKL21_11745"
     CDS             complement(506..1045)
                     /locus_tag="VKL21_11745"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMB46481.1"
                     /translation="MFKIMNVFSMHSIQIIRYLGRRYRDGYYVREISRNLSISLGSTS
                     QSLRTLEEAGLVSKEHKGRLVIYRANMQNFLLREFKILLTLLEIDPFILRLKEVSSRS
                     ILFGSCAAGEDTIDSDIDIFIESDNKREVARLHDSYQEKITRKISPIILDSSEFRNLE
                     AKDRPLFERINMGKVIHEA"
     gene            1374..2027
                     /locus_tag="VKL21_11750"
     CDS             1374..2027
                     /locus_tag="VKL21_11750"
                     /inference="COORDINATES: protein motif:HMM:NF023335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HMB46482.1"
                     /translation="MLVMLHYDFWQSAANFDSIEKSIKKIGDNLILTQEMIETLEFLI
                     DRISHKELDIDLPYSQPLKLHSRYTREQILAAFGANTFQKQSSNREGLVNLESKNTEL
                     LLVTLEKTEDNYSPTTMYNDYAVSEKIFHWQSQNSSKPEIGRGLSYITHKEKGKSILL
                     FVREKNTDEFGNTMSYVFLGAVNYIEHYGSKPMSIKWDLQVPIPPYIWKETAKMAVG"
BASE COUNT          784 a          462 c          473 g          814 t
ORIGIN      
        1 tgtgttttat ggcttcaagc aggcgggttt tcagggatga gaggagggtg tttaatgtga
       61 tggatatcac cattaaattt tgattagatt aattctttac atacctcaaa tattttgctt
      121 gctgaattcc gcaattccag agcagaatct tcactatata cgcttccata atccgccact
      181 tctcggatct tcatggcata gctaaaatcc tgcattagtc ggttatccaa agtgcctgca
      241 tccacaaata gagactcgat tgcgtatttt atgcatgtgt ggcttttttc tctgtagcct
      301 ttgctgaaga ttagagctct aaatgaatgg aacatagaat aataagcctg gattatcgcc
      361 catttgaaat tcctctcctg caaagatctt tcacatgaat caagatcgtt ttttgcttca
      421 ttgatctctt ttgatataat gtcaggtgca atttcaatct ttactatttt ccctctttca
      481 agacactgtt caaactgata tctcattatg cttcatggat tacttttccc atgttaatcc
      541 tttcgaaaag tggtctatct tttgcttcaa ggttcctgaa ttcagatgag tccagaatga
      601 tgggagatat cttcctggtg atcttctcct gataactatc atgaagcctg gcaacttctc
      661 ttttattatc ggattcaatg aaaatatcga tatcgctgtc tattgtatcc tctcctgcag
      721 cacagcttcc aaacaggatg ctccttgaag agacttcttt aaggcgaagg atgaacggat
      781 ctatttccag aagagtgaga agtatcttga actctctgag caggaaattt tgcatgttcg
      841 ccctgtatat taccagcctg cctttgtgct cttttgaaac tagtcctgct tcttcgagcg
      901 ttctcaggct ctggctcgtt gaccccagac tgatactaag atttcttgag atctcacgga
      961 catagtagcc atctcgatat cttctaccga ggtagcggat tatctgtatt gagtgcattg
     1021 agaatacgtt cattattttg aacatatgtt catttatatg aacaaaggta tttaagattt
     1081 tcgggtgggt ttgatgagtt gattgcttcc ttttcgacca tgtgcgctat cttcgcggtg
     1141 atggtcgttg tcttgccagt acctgcgcct gcgaggatca ggtggggggg ttgggtgtgg
     1201 attgagagga gctggcggga ttttaaattt aggaggatgg agtctaacaa gtatcaacca
     1261 ctttttcccc ctctttcaat tttttaacta aagaaagtta tttaactttc atactgaacc
     1321 ttgcaaaaaa taatttttct attccctcaa caataaatga aaatgaaaaa ctaatgttgg
     1381 tgatgttaca ttacgatttt tggcaaagtg cagcaaattt tgacagtata gaaaagagca
     1441 taaaaaaaat tggcgataat ctaatattga cacaagaaat gatagagact ttagagtttt
     1501 tgatcgacag aatatctcat aaagaacttg atattgatct accttattca caacctctaa
     1561 aattacatag ccgctataca cgagagcaaa ttttagcagc atttggtgca aacacttttc
     1621 aaaaacaatc aagtaataga gaaggacttg ttaatttgga atcaaagaat acagaattgc
     1681 tcttagttac tttggaaaaa acagaggaca attattcgcc aacaactatg tacaatgatt
     1741 atgcagttag cgaaaaaata tttcattggc aatctcaaaa ttcatcaaaa ccagaaatag
     1801 gtagaggttt gtcatatatt actcataaag aaaaaggtaa atcaatttta ctttttgtaa
     1861 gagaaaaaaa tacagatgaa tttggaaata caatgtctta tgttttttta ggtgctgtga
     1921 actacataga acactatggt tctaagccaa tgagcattaa atgggattta caggtgccaa
     1981 tccctcctta catctggaaa gaaactgcaa aaatggctgt gggttaactc caatgagcaa
     2041 caatcgaatc atcctagtgt cctgtcaagg ctaaattgtc aaatgatatc gagataatta
     2101 taattttccg caaatcatat gaaaaaatcg tgatcgattg ttatatggaa aaaataacat
     2161 aaacaatata aggatgcggt gcaatccagc gcatgcagga atactatatt cggcttttga
     2221 aaaatttagc cgcagatgaa tgcggataaa cgcagattta atgctacggt ttttgaaaaa
     2281 tagttacacc aaaaccatag aacttcacgc gccgatacgt tgcagaagca accacaagtg
     2341 aaaaactgga gaaacaagag gagtacagat caatttatct cgattatttg ctgctatagt
     2401 caagttctta aataatcgta aattggtatt ttgacaaaat cctccttaaa agcagagata
     2461 agttgctctt tcaaatcctc tttagtgttc caatttctta tcgccagcca cctttttgtt
     2521 tctctccagg act
//
LOCUS       DATGBN010000461         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_308399, whole genome shotgun sequence.
ACCESSION   DATGBN010000461 DATGBN010000000
VERSION     DATGBN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308399"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /gene="nuoB"
                     /locus_tag="VKL21_11755"
     CDS             <1..278
                     /gene="nuoB"
                     /locus_tag="VKL21_11755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HMB46483.1"
                     /translation="GTVTKRMAPRVKQLYEMMADPKWVISVGSCANTGGPYHDSYSVL
                     KGVDLIIPVDVYIPGCPPRPEAWIYGIIQLREKIKNKGYGAKWGLNK"
     gene            275..775
                     /locus_tag="VKL21_11760"
     CDS             275..775
                     /locus_tag="VKL21_11760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMB46484.1"
                     /translation="MNPAEEIKQLFPGSVLSTGFQTNKPLAIIKKEDLLIIAEKVKEM
                     GFDNLSLITGIDYRDRFEIIYNFFSYAKKQNLVLKVVLDHLNPEVDSLESHWKVADWL
                     ERETYDLVGIKFRGHPNLKRILLPEGWVGHPLRKDYDMSVDQFVTVGEDGEDIVSTDG
                     SNLLRR"
     gene            777..1925
                     /locus_tag="VKL21_11765"
     CDS             777..1925
                     /locus_tag="VKL21_11765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMB46485.1"
                     /translation="MKTEELLINMGPVHPSTHGVLRFKLTMDGEIIKKCEIVMGYLHR
                     GTEKLGEMKTYLQFVPYTDRLDYISAFPNNYAYVKAVEELAGIEVPLRAEYIRVLTLE
                     LNRVVNHLVMLGSLLLDLGGSTMIMWGFRERENALQLFEMLCGARMTFNYIRPGGVRE
                     DLPEGFMPKLNEFIKDMEYRIDDYEQLISGNEIFMMRMKNIGVLSAKDAISYGMTGPS
                     LRGHGVNYDIRKVEPYSVYPDLDFRACVRNECDSFARYMVRIDEMRESLHILRQVVDA
                     MPEGKPTSQAKYGIAPMTGAPTAVSTYFPRIFTPPPGEVYSRIEAPKGELGFYVVSDG
                     TIKPYRIKIRSPSFCNLTAIPKMVEGLMIPDLIAAFGTIDVVLGDVDR"
     gene            1930..3036
                     /gene="nuoH"
                     /locus_tag="VKL21_11770"
     CDS             1930..3036
                     /gene="nuoH"
                     /locus_tag="VKL21_11770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMB46486.1"
                     /translation="MFANAYELIVYILNFIGIYEPINNIFKMLGIEYVLRIIIVFVEI
                     AILLVFALLTIMFLTWMERKVVAMVQARYGPMVTGPRGLLQPVMDGIKLIGKEDIIPA
                     KADRWIFTLAPFLIFIPALLIFIPIPFGESLIISDLNVGLLYIIAISAVSPIAILLAG
                     WASNNKYSLMGAIRAVAQDISYTIPLALSAIAIVLFTGSLSTVEIVSAQSETWFGFIP
                     KWFIFLQPLGFLLFLIASIAEMGRIPFDFQESEQELVAGFFTEYSGMKFAMFFLAEYA
                     HLFSVSAIVVTLFLGGWSFPFLTVLPAALVPIVSLGIFLIKTYAVIFFAMWLRDTVPR
                     VRIDQLMNLGWKIMIPLALLNLLATGFLLTVGVF"
     gene            3036..3452
                     /locus_tag="VKL21_11775"
     CDS             3036..3452
                     /locus_tag="VKL21_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HMB46487.1"
                     /translation="MAWINKNWTMFGIVKGLIVTMKHIIRIGNRPTVTVQYPYQKMKV
                     WPRFRGLHAVDENACIGCGICEVSCPNGTIQIVKYKTKWYPQVNIGMCLFCGLCVDAC
                     PMNAMKMTGNYELADYTRESLVYGPDRLLFNKEVKQ"
     gene            3449..3694
                     /locus_tag="VKL21_11780"
     CDS             3449..3694
                     /locus_tag="VKL21_11780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMB46488.1"
                     /translation="MIDIAFLVISVLTIISTIMVVRSNKLVHSATYLAGTFIGTAGLF
                     ILLKAEFLAWVQVLVYVGAVITLILFTIMLTRKGEEE"
     gene            3696..3962
                     /locus_tag="VKL21_11785"
     CDS             3696..3962
                     /locus_tag="VKL21_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46489.1"
                     /translation="MINKTFNLLIALLLFATLFLAIDNSFTDLAKKEEITPISIEEIA
                     GGMNNTGETIRHGIFSDFIFPFELLSLLLLASLMGALYIAKKEA"
     gene            3964..4263
                     /gene="fpoK"
                     /locus_tag="VKL21_11790"
     CDS             3964..4263
                     /gene="fpoK"
                     /locus_tag="VKL21_11790"
                     /EC_number="1.12.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051911 - Methanosarcina-phenazine
                     hydrogenase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F420H2 dehydrogenase subunit FpoK"
                     /protein_id="HMB46490.1"
                     /translation="MSLYLYLLISIIIFTIGVYGVITQRSGIKILMCIELLLNSANLN
                     LVAFSSYNGNATGQVFAIFSIAIAAAEAAIGFAILVALFRLRDTINLDNINILRW"
     gene            4265..>4736
                     /locus_tag="VKL21_11795"
     CDS             4265..>4736
                     /locus_tag="VKL21_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMB46491.1"
                     /translation="MFSEFAALIVLLPVIAFVLTLFLGKKLYSGGALLPIAAIAGSFI
                     ISVGIFFEIFPNDGMVQQSFPWFAGMNIGILIDPLAVVMLMMVTFVSLLIHIYAVGYM
                     SHDPARPRYFAETSLFTAAMLSVILSDNILQFFISWELVGLCSFLLIGFWYHKPS"
BASE COUNT         1343 a          896 c         1096 g         1401 t
ORIGIN      
        1 ccgggaccgt tacaaaacgc atggctccca gagtaaaaca actctacgag atgatggctg
       61 atccaaaatg ggtcatatca gtaggcagct gcgcaaatac aggaggccct tatcatgatt
      121 catattcagt attaaaaggc gtggatttga tcataccggt tgacgtttat attcccggat
      181 gcccgccaag acccgaggca tggatttacg ggatcattca gttgagggaa aagataaaga
      241 acaaaggata tggggcgaaa tgggggctga ataaatgaac cctgctgaag agataaagca
      301 attatttccg ggttctgttc tcagcacagg cttccagaca aataaacctc ttgcgatcat
      361 taagaaagaa gacctgttaa ttattgctga aaaagtgaaa gaaatgggat ttgacaatct
      421 ttccctgata acaggcattg attataggga caggttcgag ataatttata atttcttttc
      481 ttacgctaaa aagcagaacc ttgttttaaa agttgttctc gaccatttga atcctgaagt
      541 cgattctctt gaatcccact ggaaagtggc agattggctt gagagggaga cctatgatct
      601 tgtggggatc aagttccggg gccatccgaa tctaaaaagg atattattgc ctgaaggctg
      661 ggtcggccat cccctcagaa aggattatga tatgagcgtg gatcaattcg ttaccgtagg
      721 ggaagatgga gaagatatcg tgagcactga cgggtcaaac ctgctgagga gataaaatga
      781 aaacagaaga acttctaatt aacatgggtc cggtccatcc aagtacacac ggtgtattga
      841 ggttcaagct aacaatggac ggggagataa tcaaaaaatg cgagatcgtt atgggatatc
      901 tccatcgcgg aacagaaaaa ctgggagaga tgaagacgta tctgcaattc gttccttata
      961 cggacaggct cgattatata tccgccttcc caaacaatta tgcttatgtc aaagctgtgg
     1021 aggagcttgc aggtatagag gtcccattga gggcggaata tattcgagtc cttacgcttg
     1081 aactgaacag ggttgtgaat cacctggtca tgctgggctc tcttcttttg gatctaggtg
     1141 gatctacaat gataatgtgg ggtttccggg aaagagagaa tgccctgcag ttattcgaga
     1201 tgctatgcgg cgccaggatg acttttaatt atatcaggcc aggaggagta agggaagacc
     1261 ttcctgaagg cttcatgcca aaacttaacg aattcataaa agatatggaa tatcgtatag
     1321 atgattacga gcagctcata agcggaaatg agattttcat gatgagaatg aaaaatatcg
     1381 gtgtattaag cgcaaaagat gctatcagtt atggtatgac aggtccgtcg cttcgtggtc
     1441 atggcgtgaa ttatgatata aggaaagtcg aaccgtattc agtgtatcct gaccttgact
     1501 tcagggcatg cgttcgcaat gaatgcgaca gctttgccag atacatggta agaattgatg
     1561 agatgagaga aagcctgcat atcctgagac aggtggttga tgcaatgcct gagggaaaac
     1621 caacaagcca ggcaaagtac gggatagctc cgatgacggg cgctccaacg gcagtttcca
     1681 cgtattttcc gagaatattt actccgcccc cgggagaggt ttacagcagg atagaggcgc
     1741 caaagggaga attgggattt tatgtcgtaa gtgatggaac aattaaacca tacaggataa
     1801 agatacgctc accttctttc tgcaatctta ctgccatacc aaaaatggtt gaaggactta
     1861 tgatccctga ccttatagca gccttcggga caattgatgt tgttcttggc gatgtggacc
     1921 ggtgatataa tgtttgcaaa tgcttacgaa cttattgtgt atatcttgaa ttttatcggg
     1981 atttacgagc ctataaataa tatttttaag atgctgggaa ttgagtatgt tttgaggatc
     2041 atcatcgtgt ttgtggagat agcaatctta ctggtttttg ctcttcttac gatcatgttc
     2101 cttacatgga tggaacgtaa agttgtcgcc atggtacagg ccaggtacgg cccaatggtc
     2161 acaggaccgc ggggattact ccagccagtc atggatggta tcaaactcat aggtaaggag
     2221 gacataatcc cggcaaaagc agacagatgg atttttacgc ttgcgccctt tctcatattc
     2281 ataccagcac tcctcatttt tatcccgatt ccttttggcg aatcgctcat aatatcggat
     2341 ctgaatgttg gtcttcttta tataattgcg atatctgccg tttccccgat cgcaattttg
     2401 cttgcaggct gggcttcaaa taataaatat tcattaatgg gggcaataag agcagtggct
     2461 caggacataa gttatacgat cccgcttgcc ctttctgcta tagctattgt tttattcacc
     2521 ggttccctga gcacggttga aatcgtatct gcgcaaagcg agacctggtt tggctttatt
     2581 ccaaaatggt tcatattcct gcagcccctg ggttttcttt tattcctgat agcatcgatc
     2641 gcggaaatgg gtcgtatacc ttttgatttc caggaatcag aacaagaact tgtagctggt
     2701 tttttcactg aatacagcgg gatgaaattc gctatgtttt tccttgcaga gtatgcacat
     2761 ttattttcgg tatctgcaat cgttgtcacg ctgttccttg gaggatggtc atttccattc
     2821 ctcactgtgc ttccggcagc attagtccct attgtttcgc tgggaatatt ccttataaag
     2881 acatatgcag taatattctt cgctatgtgg ttaagggata cagtacccag agtcaggata
     2941 gaccaactta tgaatctggg ctggaaaatc atgatccctc tggctttact taacttactg
     3001 gccacaggtt tccttttgac cgtaggggtg ttctaatggc ctggataaac aagaattgga
     3061 ctatgttcgg gatcgttaag gggcttatag ttaccatgaa acatataata cgtattggta
     3121 acaggcctac tgtgactgtc cagtatccat atcagaaaat gaaagtatgg cccagattca
     3181 gggggctgca tgcagtcgat gaaaatgcct gcattggttg cgggatatgt gaagtgagtt
     3241 gtcctaatgg aacaatacag attgtaaaat acaaaactaa atggtatccg caggtcaata
     3301 tcggtatgtg cctgttctgc ggcctttgtg tggatgcatg ccccatgaat gccatgaaaa
     3361 tgacaggaaa ttatgagctt gcggattaca cgcgtgagag tcttgtgtat ggtcctgata
     3421 gattattatt caataaagag gtgaaacaat gatagacatt gcatttctgg ttatttcagt
     3481 tctcacgatc atatcaacta ttatggtggt cagatcaaat aaattagtcc acagtgcaac
     3541 atacctggca ggaacattca ttggtacagc tggtcttttc atcctcctca aagccgagtt
     3601 ccttgcctgg gtacaggtcc ttgtctatgt tggggcggtc ataaccctga tattgttcac
     3661 aattatgttg accagaaagg gggaggaaga ataaaatgat caacaaaacc ttcaatttac
     3721 tgatcgcatt actgttgttt gcaacgttat ttcttgccat tgataacagt tttactgacc
     3781 tggcaaagaa agaggaaatc acccctatca gcatagaaga aatcgcaggt ggaatgaata
     3841 atacagggga aacgataaga catggtattt tcagcgattt tatttttccc tttgaattac
     3901 tttcactgtt attattagca tcgttgatgg gcgcccttta tatcgccaag aaggaggcat
     3961 aaaatgtctt tatatttata tctcttaatt tcaataataa tattcaccat aggtgtatat
     4021 ggagtaatta cacaaagaag cgggatcaaa atcctgatgt gcattgaatt gcttctgaat
     4081 tcggcaaatc ttaaccttgt tgctttctca agttataatg gtaatgcgac aggccaggta
     4141 tttgcaatat tttcaattgc catagcagca gcagaagctg ccataggatt tgcgatactt
     4201 gtggctttgt tcaggttgcg agatactatt aaccttgaca atatcaacat attgaggtgg
     4261 taatatgttc agcgaatttg cagcactaat agtattatta ccggtcatag catttgtatt
     4321 aacgcttttc cttggtaaga aactctattc aggcggagca ttattaccaa tagcggcgat
     4381 cgcaggttcc ttcatcatct cggtcggaat attttttgag atatttccta atgatggaat
     4441 ggtccagcag tcatttccct ggtttgccgg aatgaatatt ggaatattga tcgatccact
     4501 ggcggtagtt atgctgatga tggtcacatt cgtcagtctt ctcattcata tttatgcagt
     4561 gggatatatg tcccatgatc ctgcaagacc cagatatttt gctgaaacat cactttttac
     4621 tgcagcgatg ttaagtgtaa tattatcaga taatatcctt cagttcttta tttcatggga
     4681 attggtagga ttatgttcat tcctattgat aggtttctgg taccataaac catcag
//
LOCUS       DATGBN010000462         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_192082, whole genome shotgun sequence.
ACCESSION   DATGBN010000462 DATGBN010000000
VERSION     DATGBN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192082"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..541
                     /locus_tag="VKL21_11800"
     CDS             176..541
                     /locus_tag="VKL21_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMB46492.1"
                     /translation="MKTTRKSFIREILKVTQQPEVISFAGGLPNPGFFPVEEIARAST
                     KVLADDGRNVLQYSTSEGYLPLREFIAGRYLRKNGLKIEPDDILITNGSQQGIDLIGK
                     VFLNKGDKVVIPSLSKILQ"
     gene            957..>1623
                     /locus_tag="VKL21_11805"
     CDS             957..>1623
                     /locus_tag="VKL21_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HMB46493.1"
                     /translation="MIKKMSSQRWVVIERPGYLGAIQAFSIFEPNFQSVPLLDDGIDT
                     DLLEKALSEDNAKLLYAIPNFQNPSGITYSGQKRKDVANIIKKYNVVCIEDNPYGELR
                     FTGEDLPSIRNYSDNTILLGSFSKIISPGLRIGWICAGKEIMEKLIIAKQAADLHSNS
                     LSQRIVHQYLMDNDIDEHIMKLRAAYGKRRDLMIDMMTEYFPEEVKYTKPDGGMFLWV
                     TLPE"
BASE COUNT          550 a          276 c          315 g          482 t
ORIGIN      
        1 caaccttcga ttggattata gaggaaatgc tccataaatc tgtatttaaa tttgacattc
       61 ttcataagga tgattttatt gcagtatatt actgcaagaa ggaaaaagcc tcgcaatcgc
      121 ctgatagtac tcatatgaaa aggatatctg catgaacaat agatttgctg acagaatgaa
      181 gactacccgt aagtccttca tccgggagat attgaaagtc acccagcagc cggaagtgat
      241 atcatttgcc ggcgggcttc cgaatcccgg ttttttcccg gtggaagaaa tagcaagagc
      301 ctccacaaag gttctggcgg atgatggcag gaatgttctg caatacagta cgagtgaagg
      361 atatttacct ttgagagagt tcattgctgg aagatatcta aggaagaacg ggttaaaaat
      421 agaacctgac gatatattaa ttactaacgg ttcacagcag ggcattgact taattggaaa
      481 ggttttcttg aacaaaggag acaaggttgt tatcccaagt ttgagtaaaa ttttgcaata
      541 aaatcaacaa aatcaaaatc taatgacact atcatctttc taaaaccaca aaaaaatgta
      601 ggtacaactc ttacaacttc aatttttttc acttgatctt gatatctgct tttttatgga
      661 aaattttatc ttgttacaaa ctattaattt gttgtcagag aatatcaaaa aatactattg
      721 cagtaatgca tatcgaaatc tcagatagta tatgtaatgt gtatacttat ttaaatattt
      781 tatgcttcag caataaccga ttttttcaat aaacttgtat acctacatat ccaacagatt
      841 aaataattgg ttgaagttaa accgatatct accgacgtgt ttattgaagc aaaaaatcaa
      901 tcgaattgaa taaaaattag attgtttttc acaaaatgtg ccgatggata cctattttga
      961 ttaaaaaaat gagttctcaa agatgggttg ttatcgaaag accaggatac ctgggagcaa
     1021 ttcaggcatt ttctatcttc gaaccaaatt ttcaatccgt tcctcttcta gatgatggaa
     1081 tagacactga tttactggaa aaagcattga gcgaagataa tgcaaagctc ctttacgcca
     1141 ttccaaattt ccagaatcca tccggtatta cctactccgg acaaaaaaga aaagacgttg
     1201 ccaatattat caaaaaatat aacgtcgtct gcattgagga taatccatac ggcgaattaa
     1261 gatttaccgg ggaagatttg ccttccatca ggaattattc ggataataca attcttttgg
     1321 gttcgttttc aaaaataatc tctccgggat taagaatcgg gtggatatgc gccggaaaag
     1381 aaataatgga gaaattgatt atcgcaaagc aggctgctga cttgcattcg aattcccttt
     1441 cacagaggat tgttcatcaa tacctgatgg ataacgatat agacgaacac attatgaaat
     1501 taagggctgc ctatggaaaa cggcgggatt tgatgattga tatgatgact gagtacttcc
     1561 cggaggaggt taagtacaca aaacctgatg gcgggatgtt tttgtgggta acgcttcctg
     1621 aga
//
LOCUS       DATGBN010000463         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_502537, whole genome shotgun sequence.
ACCESSION   DATGBN010000463 DATGBN010000000
VERSION     DATGBN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502537"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VKL21_11810"
     CDS             <1..223
                     /locus_tag="VKL21_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46494.1"
                     /translation="KRSIETADVKEQRNKATICATVSPWLKNRLERMVDGKEFSSVSD
                     IVSLACHEFLIRHFPDQDERPVRESEKKK"
     gene            complement(424..1632)
                     /locus_tag="VKL21_11815"
     CDS             complement(424..1632)
                     /locus_tag="VKL21_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HMB46495.1"
                     /translation="MEYPERQKPTPGGEVSSVPPGVDISSVSLVKCQDYESSNIEAAI
                     EKALKLIGGIGSFVKPGDSVLLKVNLLTGDVPEKAVTTHPAIVRAMIHQVKAAGGIPQ
                     VGDCSGYEGASNPKRYFAACRSSGIMQVCEEEKVEVLHLSAQSVDTKNPGGRIFKSFI
                     LSKHVLQADVLISLPKLKTHGLTLFTGGVKNIFGCVTGLNKAKMHLRAQDPETFSQML
                     VDLLGLVQPELTVMDAVVGMEGNGPSNGTSRQINAILASKDPVALDAVACKIVGIDPF
                     MVPSTRLAHEQGLGIGDISMINVMGENPGNMEIKDFRLPDGTASFFRARGLMRILRSM
                     LVAKPVLVKERCKKCWICMEHCPSEAISKKGVYPSFDHKKCIRCYCCQELCPGDAIEL
                     RMPFLGRFVK"
     gene            complement(1641..2156)
                     /locus_tag="VKL21_11820"
     CDS             complement(1641..2156)
                     /locus_tag="VKL21_11820"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMB46496.1"
                     /translation="MDVYKAILSRRSIRENESHDVEEDKLERVLESGRLSPSAGNRQE
                     RRFVVIRDAGTRKALSEAAKGQVFVAQAPVVIAACSVESEYVMSCGQLAYSIDTAIAV
                     DHITLQAVEEGLGTCWIGAFDEKKVKEILNIPGNVRVVALLPLGYPHVVPHPTARKSL
                     SEIVMREKWRI"
     gene            2389..>2966
                     /locus_tag="VKL21_11825"
     CDS             2389..>2966
                     /locus_tag="VKL21_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMB46497.1"
                     /translation="MVNEKLVRFKIKRFDGKKSRYDRFEIEPKPHMSVLEALFQIQDA
                     QDSTLAFRYSCRGAVCGSCAMLINKKHRLACRTQVSEIVGEKVLSISAYGPLAKPEFK
                     TGDEILVEPLPNLPLIKDLIVDMKPFFKHYESLKPWFEGKQEVLMTQKDTINVDKYAS
                     CILCGLCYGACPVSARDKDYLGPSTLAKAMRF"
BASE COUNT          821 a          691 c          604 g          850 t
ORIGIN      
        1 taagagaagc atagaaacag cagatgtaaa ggaacaacgc aacaaggcta ccatatgcgc
       61 tacagtgagt ccgtggctga agaataggct cgaaagaatg gtcgatggca aagagttctc
      121 atcagtgagc gacatcgtca gcctggcatg tcatgagttc cttatcaggc acttccctga
      181 tcaagatgag cggcctgtac gtgaaagtga aaagaagaaa taaatagagc gtgggatgga
      241 cacaggaagc ggtgcccatc tgttagcttt tgcaaataat tagtctccaa cttaattttt
      301 tttgatttca gtcttatcgt tcatctttat tccgatttcc ttcgaacatc cacagtagaa
      361 gatatgtttg gatttattat tactttcacc ccgctcagaa agagattatt acttttcctg
      421 aaattatttc acgaacctac ccaaaaaagg catcctcaat tcaatggcat cacccggaca
      481 taattcctgg cagcaataac atcttatgca cttcttatga tcaaatgacg gatacactcc
      541 ttttttagaa atagcctcag aagggcagtg ttccatgcat atccagcatt ttttgcatcg
      601 ttctttcacg agcacaggtt ttgccaccag catactgcga agtatccgca tcaaacccct
      661 ggcacggaaa aatgatgcgg taccatctgg aagccgaaag tccttgattt ccatattccc
      721 gggattctca cccattacat tgatcatgga aatatctcct atccccaggc cctgttcatg
      781 cgccaggcgg gtagaaggga ccatgaacgg atctattccc actatcttgc aggctacggc
      841 atcaagcgcc accgggtctt tacttgccag gatcgcattg atttgtcttg acgtaccatt
      901 gcttggcccg ttcccttcca taccaaccac tgcatccatt acagtcaatt caggctgcac
      961 gaggccaagc agatccacaa gcatctgtga aaaagtttcg ggatcctgtg cccgaagatg
     1021 catttttgcc ttattgagac ccgttacaca accgaagata tttttcaccc ctcccgtaaa
     1081 taatgtcaat ccatgggttt tgagttttgg cagggagata agcacatcag cttgcagaac
     1141 atgctttgac aaaataaaac tcttaaatat tcgacccccg ggattttttg tatcaacgga
     1201 ttgggcgctc aggtgtaaga cttctacttt ttcttcctcg catacctgca taataccgga
     1261 actgcggcat gcagcaaagt acctcttggg atttgacgcg ccttcatatc cactgcaatc
     1321 gccaacctgt gggatgcctc ctgctgcttt tacctggtgg atcatggcac gtacgatagc
     1381 cggatgggtg gtaactgcct tttcaggaac atcacctgtc aggagattga ccttcaaaag
     1441 gacactgtca ccaggtttta cgaaacttcc aataccgcca ataagcttga gtgctttttc
     1501 tatcgcagcc tcaatatttg aagattcata atcctggcac tttacaaggg aaacagaaga
     1561 aatatcgact cctggaggta cggaagagac ttcacctcct ggtgtgggtt tttgcctctc
     1621 tggatattcc atagaaggct tcaaatcctc catttttccc gcataacaat ctcactaaga
     1681 ctttttcttg ccgttgggtg cggcaccaca tgaggataac caagaggcaa aagcgcaacc
     1741 actcgcacat taccaggaat attcaatatt tctttcactt ttttttcatc gaatgcgcct
     1801 atccagcatg tgcccaaccc ctcttcaact gcctggaggg ttatatgatc cacagcgatc
     1861 gcagtatcaa tggaataagc cagttgcccg caagacataa catattccga ttcaactgaa
     1921 caggctgcaa ttactacagg cgcctgggca acaaatacct ggccctttgc cgcttctgaa
     1981 agtgcttttc gtgttcctgc atccctgatt acaacaaacc tgcgctcctg gcggttccct
     2041 gcagaaggag agagtctgcc tgattcaagg actctttcca atttatcctc ttcaacatca
     2101 tgggattcat tctcccgaat gcttcttctt gatagtattg ctttatacac atccatattc
     2161 tttcctccct tattttttca aattattttg atatatgact attgttccat catgtttatg
     2221 gttttttagt atcatagtct ttattgatga gcgtttgggt tgaagaaata gtattcatca
     2281 cgtgttccta aatcatttgt ctctgtttat actttaagag agagctggca ggactgttca
     2341 acaaacaaag ttttaataga ttgccaaaca atagctatag atgggattat ggttaatgaa
     2401 aaacttgtca ggtttaaaat aaagagattc gatgggaaga aatccagata tgatcgattt
     2461 gaaatagaac caaaacctca catgtcagtg ctggaggcac ttttccagat acaggatgca
     2521 caggacagca cccttgcatt tcgatattca tgccgtggag cagtatgcgg ctcatgcgct
     2581 atgctgataa ataaaaagca tcggcttgcc tgccggacac aggtgtctga aattgttggt
     2641 gagaaagtac tttcaatttc agcatatggc cctcttgcaa aaccggaatt taaaacaggg
     2701 gatgaaatcc tggtagaacc attacccaac cttcctttga tcaaagatct tatagtggat
     2761 atgaagcctt ttttcaaaca ttatgaatct ttgaagccat ggtttgaagg caagcaagag
     2821 gtcttaatga ctcaaaaaga cactataaat gttgataaat atgcctcctg tattctgtgc
     2881 ggcttgtgct atggggcgtg tccggtttcc gcaagagata aagattatct gggtccttcc
     2941 acgcttgcaa aagcaatgcg gttcta
//
LOCUS       DATGBN010000464         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_425478, whole genome shotgun sequence.
ACCESSION   DATGBN010000464 DATGBN010000000
VERSION     DATGBN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425478"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /gene="porA"
                     /locus_tag="VKL21_11830"
     CDS             complement(<1..1087)
                     /gene="porA"
                     /locus_tag="VKL21_11830"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorA"
                     /protein_id="HMB46498.1"
                     /translation="MRKNMVVVEGSYAVAHAVKVCKPNVISAYPITPQTHIVEDLAQF
                     VADGDMNCEYMNVESEFSAISALIGASATGARTYSSTTSQGLALMHEALFNASGMRLP
                     IVMTVVNRALSAPINIWNDQQDSISQRDTGWIQLYAEDVQESADMTPQIYKLAEDEDV
                     LLPAMSCMDGFILSHVYEPVILLEQSLTDEFLPAYNPEFVLDPKNPMSFGAFADPSTY
                     TEFRYKQEKAMGVALKKIEKIANEFRDIYGRYYGGLIDGYVLEDAQIVIMAMGSIIGT
                     IKDTIDELRKEGESVGLLKVRSFRPFPTDAIRKALKNARVVITLDKNISIGKNEGALC
                     TEVKACLYNTDNRVPVIGFMLGHGGRDI"
     gene            complement(1084..1344)
                     /gene="porD"
                     /locus_tag="VKL21_11835"
     CDS             complement(1084..1344)
                     /gene="porD"
                     /locus_tag="VKL21_11835"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorD"
                     /protein_id="HMB46499.1"
                     /translation="MKLVIRTVVKPGSTRANKTGAWRSFMPVFDHKLCSKCGICAIYC
                     PEGIVNKLENGYFEPDYEYCKGCGICANECPKKAIVMVLEGK"
     gene            complement(1341..1886)
                     /locus_tag="VKL21_11840"
     CDS             complement(1341..1886)
                     /locus_tag="VKL21_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HMB46500.1"
                     /translation="MKEIRIHGRGGQGSVTAAELLAVAAFEDGKYSQAFPAFGVERRG
                     APVTAFVRLDDKPIRLRSQIYEPHYVIVQDATLVDVVNIAAGTKPDGFILINTEKSPT
                     FFNLDTKAIVKTLDATKLAMDIIGKPIVNTILTGAFAGVSGLINPESIKNAVMERFPG
                     KVGEKNSQAIQAAYDLMEAQR"
     gene            complement(2055..>3017)
                     /gene="carA"
                     /locus_tag="VKL21_11845"
     CDS             complement(2055..>3017)
                     /gene="carA"
                     /locus_tag="VKL21_11845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMB46501.1"
                     /translation="YKGQILMFTYPLIGNYGISGETFQSNCMQAEGLVVREACDHPSH
                     YMSKRSIYEYLRDEGKSGIMGIDTRMLTIKTRERGTMKAAIIVGSDDGREAVRLAKEQ
                     EDIGSLDLIGRVTCKQPYQLTGSEDGDNIVVMDFGVKRNIIKSLNNRGFNVTIVPATM
                     PLKDILNYEPDAILLSNGPGDPQQAVNGISVVKELAGQLPIFGICLGHQIISLALGAE
                     TYKMKFGHRGANQPVKDLKRGIVYITSQNHGYAVDESSIDTKELTITQFNTNDKTVEG
                     LQHRDLDIMSVQYHPEAHPGPLDTEKIFFDRVVETTKKIKVLAK"
BASE COUNT          746 a          723 c          616 g          932 t
ORIGIN      
        1 gaatatcgcg ccctccgtga cctaacatga aaccgataac tggcactcta ttatctgtat
       61 tgtaaaggca tgctttgact tcagtgcaaa gagccccctc attcttgccg atcgaaatat
      121 tcttatcaag agttatgacc actcttgcat ttttcaatgc tttcctgata gcatctgtcg
      181 ggaacggcct gaaagacctc actttgagaa ggccaacact ttctccttct tttcgaagtt
      241 catctatagt atcctttatg gttccaatga tcgaacccat tgccataatg actatctggg
      301 cgtcctcaag tacatagcca tctataagac cgccgtaata tcttccgtaa atatccctga
      361 attcatttgc gatcttctct attttcttta aagcaacacc cattgctttt tcctgtttgt
      421 atcgaaactc agtatatgta ctgggatcag caaaagcccc gaaagacatg ggatttttcg
      481 ggtcaagaac gaattcggga ttataagctg gaaggaattc atcagtgagg ctctgctcaa
      541 gtaaaattac aggttcataa acatgggaaa ggatgaaccc gtccatgcag gacattgcgg
      601 gaagcaaaac atcctcatcc tcggcgagct tatatatctg cggggtcatg tctgcggatt
      661 cctggacatc ttcagcatag agttgtatcc agcccgtatc cctctgggat atagagtcct
      721 gctggtcatt ccaaatattg atcggggctg aaagagcgcg gtttacaaca gtcatgacaa
      781 tgggtaatct cattcctgag gcattgaata atgcttcatg cataagcgcc agtccctgcg
      841 atgtcgttga tgaatatgtc cttgccccgg ttgcgcttgc gcctattaat gccgagatgg
      901 ctgaaaattc cgattcaaca ttcatatatt cgcaattcat atcaccatca gcaacaaact
      961 gtgcaaggtc ttccacaata tgcgtctggg gagtaatagg atatgctgat attacatttg
     1021 gcttgcatac cttgacggca tgggccacag cataagaacc ctctacaaca accatgtttt
     1081 ttctcatttt ccctccaaca ccattacgat ggctttcttt ggacattcgt tagcgcagat
     1141 accgcagcct ttgcagtatt cataatccgg ctcaaaataa ccattttcca atttattgac
     1201 aatcccttcg ggacaataga tcgcgcatat cccgcatttg ctgcacagtt tatgatcaaa
     1261 aaccggcatg aatgaacgcc aggcgcctgt cttattagct cgcgtgcttc ctggtttgac
     1321 cactgttcta attacgagtt tcatcgctga gcctccataa ggtcatatgc ggcctggatg
     1381 gcttgcgaat ttttctcgcc cacttttccc gggaaccgtt ccatgacggc gtttttaatg
     1441 gattccggat tgataagacc ggagacaccc gcaaaggcgc cggtaaggat cgtattaaca
     1501 ataggttttc ctatgatatc cattgcaagt tttgtggcgt caagcgtttt aactatagct
     1561 tttgtatcaa gattaaagaa tgtcggactt ttttccgtgt ttattagaat aaacccatcg
     1621 ggcttggtcc cggcagcgat attaactaca tccacgagcg ttgcatcctg tactatcaca
     1681 taatgcggtt catatatctg gctccttagg cgtatgggtt tgtcgtcaag tcgcacaaat
     1741 gcagttacgg gagcgcctct tctttcaacg ccaaacgcag ggaaggcctg gctgtatttt
     1801 ccatcctcaa atgcggcaac ggccagtagt tctgctgcag tcacagaacc ctgtccaccg
     1861 cgaccatgta ttcgaatttc cttcattttt ttctccaaga ataatagata ataattattt
     1921 aacaatcaat cgtgatgaat gtctcaaaca attgcattaa ggtatttatt ctttgcgggt
     1981 taaaactctc gctgttttaa gtaatatgtc tgacctttct tgattttcta attattccgg
     2041 gtaggttgca ttcttcattt agccagtacc tttatttttt ttgttgtctc cacaaccctg
     2101 tcaaagaaga tcttctcggt atcaaggggt ccgggatgcg cttcgggatg atactggact
     2161 gacattatgt caagatccct gtgttgcagt ccttcaactg tcttatcgtt ggtattgaac
     2221 tgggtgatcg tgagttcttt tgtatcgatg ctgctttcgt caaccgcata accatgattc
     2281 tgcgatgtga tgtatacgat accccgtttc aaatctttga caggctgatt cgcaccacgg
     2341 tggccgaatt tcattttata tgtttccgcg ccaagggcaa gggatattat ctggtggcca
     2401 aggcatatcc cgaaaatcgg gagttgtccg gcaagctctt ttacaacgga tatgccattt
     2461 accgcctgct gcgggtcacc cggcccgttg gaaagaagta ttgcatcagg ctcgtaattc
     2521 agaatatctt tcagcggcat agttgcagga actatagtta cattgaatcc cctgttgttc
     2581 aggctcttga taatatttct cttaacacca aaatccatta ccacaatatt gtcgccatct
     2641 tcactccctg taagttggta tggctgtttg catgtgaccc tgcctataag gtcaagactg
     2701 ccaatatctt cctgttcttt tgcaagtctt acagcctccc tgccatcatc actgcccact
     2761 attattgctg ccttcattgt tccgcgttcc cttgttttga tcgtgagcat acgggtatca
     2821 atgcccatta ttccggattt tccttcatct ctcaaatatt catagatgga acgtttggac
     2881 atgtaatgcg agggatggtc gcaggcttcc ctgaccacca gtccttcagc ctgcatgcaa
     2941 tttgattgga aagtttcacc gcttatacca tagttcccta tgagcggata tgtgaacatc
     3001 aatatctggc ctttata
//
LOCUS       DATGBN010000465         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_425538, whole genome shotgun sequence.
ACCESSION   DATGBN010000465 DATGBN010000000
VERSION     DATGBN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425538"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VKL21_11850"
     CDS             complement(<1..621)
                     /locus_tag="VKL21_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46502.1"
                     /translation="MIVTRHISLDNDCIQKMEPYVEKHKGNFSAAIREIIDRAGKNSE
                     LENISTIDNTLFKWMLNETDGLLVPDNVLDDLIDPMLINSMGKLEDYLKQKFSELEWD
                     VDISLKCDSDASASDVLIEVKGAPQKIRFVARVLAQYLVKNSPEHAPLEISSVFNLDG
                     CIRVELSKSNKKQGYNSLITSFGGLNEVIQAIKSRPVFWKSIINGHL"
     gene            complement(660..941)
                     /locus_tag="VKL21_11855"
     CDS             complement(660..941)
                     /locus_tag="VKL21_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46503.1"
                     /translation="MNNWKRYVAAGIVIGSVYFLPSESFMAFLTGWVILLPITAAVFL
                     AVGLREYMNDRKNRIMVSIKTTDAMKALARREEELKREKELLYEQFRKV"
     gene            complement(1119..2561)
                     /locus_tag="VKL21_11860"
     CDS             complement(1119..2561)
                     /locus_tag="VKL21_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46504.1"
                     /translation="MNITRHISLEDEYVEKLKPYVEKHDGNFGAAIRDMINQAGKNDT
                     RINSLAIDHSILNWIFKENDEKLIPSDILNEFIDPVMMNSMDNLEGYLNRRFSEFKWG
                     VFITLKNDNDIFPGEILMEISGDHQKIRFISSILTQYLVINSLRHTPLEVRSVVNTKQ
                     GIKVELSGSNNKDALISLETFFGKTDMLIRTVKSCPAFWKAIVNRHLLSNYNMVTVHR
                     NYFEDLLDGKTPEWEVTIENLAKKPIREIPLEEMLHLMKEVFETSRIVDRVEIDKDTM
                     ILFHSYRSKGAIEKLRKSCHALLESNGHMYDARATANIVIMTHRSDVGRKINEIVDNL
                     KNSSNQLDQELLVFFGYLKGLREIPDIPMSLTSLGRRTGTSLMQEYERENHIKTWTLD
                     NFQKALQDIDTRIHRVSEWKLDGKDLLYTIRKCNIAGNGNEFDNYVCHTVRETFKGAL
                     SYAFGNKAEMCTKHLLTHGDNFCEVIIRMR"
     gene            2722..>3932
                     /gene="hisS"
                     /locus_tag="VKL21_11865"
     CDS             2722..>3932
                     /gene="hisS"
                     /locus_tag="VKL21_11865"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMB46505.1"
                     /translation="MELQKPRGTRDFLPEEMIKRRYIENKLRGVAAHWGYGEIKTPAF
                     EHIELFTLKSGEGILGEIYNFKDKGDREIALRPELTAPVVRMYVEDLQRSPKPLKFFY
                     FDNCFRYERPQKGRFREFFQFGVEIIGSAKPESDAEVIALAVEMLKSAGVRGDLHVGN
                     LGIVRALINDIRPEHQSKIMRLVDKKDDKGLEEFLDTINAPDDMRGKLFRLIGLHGEN
                     AVHEAREIVGDIDAIMQFKALLDLLDVYGLEYQVDFGIARGLDYYTGMVFEIYCEGLG
                     AQNQVCGGGSYRLAALFGGEDAPSTGYAIGFDRIMEICEAKPEPAKRIVVVSFEDTRK
                     EAIVIANKLREHIDTYLDVMGRKFKDQIAYANTIGASHIIIVGRNELDAGKLALKDMR
                     TGEQELVSMEEA"
BASE COUNT         1084 a          820 c          831 g         1197 t
ORIGIN      
        1 aagatgtccg ttgattattg atttccagaa tactgggcgg cttttgatgg cctggatcac
       61 ttcattcaaa ccaccaaaag aggttatcag gctattgtat ccttgcttct tgttcgattt
      121 tgataattca acccttatac atccatcaag attaaaaacc gaagatattt caagaggagc
      181 atgctcaggt gagtttttca caagatactg ggcaagtacc cttgcaacga atcttatttt
      241 ctggggcgcg cctttgacct ctatcaatac atctgatgcc gatgcatcac tatcgcattt
      301 taaagatatg tcaacatccc actccagttc gctgaatttt tgcttgagat aatcttcaag
      361 ttttcccatc gaatttatca gcatgggatc tatcagatca tccagtacat tgtccgggac
      421 aagcaaacca tcggtttcgt tgagcatcca tttgaacaag gtattgtcaa ttgtggatat
      481 attctcaagt tcactgtttt tcccggccct gtctatgatc tctcttattg cggcgctgaa
      541 attcccttta tgtttttcaa cataaggttc catcttttgt atgcaatcat tgtcaagaga
      601 gatatgtctt gtaacaatca taattatccc actgtccctg atccttccgg aatcttcatt
      661 tacactttcc taaattgctc atagagaagt tctttttccc ttttcaattc ttcttccctg
      721 cgtgcaagcg ctttcatggc gtcagttgtc tttattgaga ccatgatcct gttcttcctg
      781 tcattcatat attcccgcag cccaaccgcg agaaaaacag ctgctgtgat cggcaaaagg
      841 ataacccatc ctgttaagaa agccataaaa ctttctgatg gcaggaaata gacacttcct
      901 attacgatcc cggctgcaac atatcttttc caattattca tatatgcttc acccaaatat
      961 gattattcaa tgccaatggt aaaagaatat ttgacagcaa gatagcatga aaagccatga
     1021 atagacaaag tttgacataa aagaggtata ttatgccatt gaatggccta tgaaatacaa
     1081 agttggcaaa acgattcagg gaattctgat aaatcaggtc agcgcattct tataatcacc
     1141 tcgcagaaat tatccccatg ggtcagcagg tgtttcgtgc acatttctgc cttatttcca
     1201 aaagcgtaac tcagtgcgcc tttgaaggtt tctcgtacag tatgacaaac ataattatca
     1261 aattcattcc cgtttcctgc aatattacat ttccttatag tgtatagcag atccttccca
     1321 tccaatttcc attcgctcac ccggtgaatt ctggtatcga tgtcctgcaa tgccttctgg
     1381 aaattatcca gtgtccatgt cttgatatgg ttctcccgtt catattcctg catcaaggag
     1441 gtgccggtcc ttctgcccag agacgtcaat gacatgggaa tatcaggtat ttcccttaat
     1501 ccttttagat aaccaaagaa tacaagaagc tcctggtcca gctggttgga actgtttttc
     1561 aaattatcca cgatctcatt gatcttcctg ccaacatcag acctgtgggt cattattacg
     1621 atgttggctg ttgccctggc atcatacatg tggccatttg attccaggag tgcatgacag
     1681 ctctttctta gtttttcaat agctccctta gacctgtaac tatggaataa tatcatggta
     1741 tctttatcta tttccaccct gtccacgatc cttgatgtct caaagacctc tttcataaga
     1801 tgaagcattt cttctaaggg aatttcccgg atgggttttt ttgcaagatt ttctatcgtt
     1861 acctcccatt ccggtgtttt tccatcaaga agatcttcaa aataattcct gtgtacagtg
     1921 accatattat aattactcaa gagatgcctg ttgacaatag ctttccagaa tgcaggacag
     1981 ctctttacgg ttcttatcag catgtctgtc tttccaaaaa aagtctcaag gctaataagt
     2041 gcatctttat tatttgaacc tgataattct acttttatcc cctgtttggt gttaacgacc
     2101 gacctgactt caagcggagt atgccggagt gaattgatga caagatactg ggtaagtata
     2161 cttgaaataa atcttatttt ttgatggtcg ccgcttattt ccatcaaaat ttcacccgga
     2221 aaaatatcgt tatcattttt taatgtgatg aaaactcccc atttaaattc gctgaacctg
     2281 cgattgagat aaccttccag gttgtccatg gaattcatca tcacaggatc aatgaattca
     2341 ttgaggatat cagatggaat aagtttttcg tcattttcct taaatatcca gtttaatatg
     2401 gaatggtcta tggccaatga attgatcctg gtatcattct ttcccgcctg gttgatcata
     2461 tcccttattg cggcgccaaa attcccgtcg tgcttctcca catacggttt caatttctca
     2521 acatattcat cttcgagaga gatatgtctt gtaatattca ttttgattcc ccaatataat
     2581 ttaatctata ttatgacaat cctaaaaggg tatttgtgag tgcattcggg ttttgtgtca
     2641 attttaggag ttttatgaca tgaaatgaca tacatgttta ttaatatcta aactatattc
     2701 aaggcaaaag gaagaattaa aatggaactc cagaaacccc gaggcacacg tgatttcctg
     2761 cctgaagaaa tgatcaagcg aaggtacatt gagaataagc tgcgtggggt tgccgcacat
     2821 tggggatatg gggagataaa aaccccggca tttgagcata tagagctttt tacattgaaa
     2881 tcgggagaag ggatacttgg tgagatatat aatttcaagg acaagggaga ccgcgagata
     2941 gcgcttcgtc ctgaactgac agctcccgtt gtaaggatgt atgtggaaga tctccagaga
     3001 tcgccaaaac ctctcaaatt cttttatttt gataattgct tcaggtatga gcgcccccag
     3061 aaaggacgtt tccgcgagtt tttccagttc ggggttgaga ttatcggaag cgcaaagcct
     3121 gagtctgatg cggaagtgat cgcgcttgcg gtggaaatgc taaaaagcgc aggtgtcagg
     3181 ggagacttgc atgttggaaa cctggggatt gttcgcgccc ttataaatga tatcaggccg
     3241 gaacaccaga gcaagatcat gcgccttgtg gataagaagg atgataaagg gctggaggaa
     3301 tttcttgata cgatcaacgc cccggatgat atgagaggaa aattgtttcg cctgataggg
     3361 ctgcatggtg aaaatgccgt tcatgaagcg agggaaattg tgggagacat cgatgcgatc
     3421 atgcaattca aagcgcttct tgatctcctg gatgtatatg gcctggaata ccaggttgac
     3481 ttcgggattg caagaggtct tgattattat accggaatgg tgtttgagat atactgtgaa
     3541 ggtcttggcg cacagaacca ggtgtgcggc ggaggctcat accgccttgc ggccctgttc
     3601 ggcggggagg acgcgccgtc aacagggtat gctatcgggt ttgacagaat catggagata
     3661 tgcgaggcaa agcctgaacc tgctaaacgc attgttgtgg tctcgttcga ggacacgcgc
     3721 aaagaggcga tagttatcgc aaataagctc agggagcata ttgataccta tcttgacgtg
     3781 atggggcgca agttcaaaga ccagatagct tatgccaata ctatcggcgc aagccatatt
     3841 attattgtgg ggagaaacga gcttgatgcg ggaaaattgg cgcttaaaga catgagaaca
     3901 ggagagcagg aacttgtgtc gatggaagaa gc
//
LOCUS       DATGBN010000466         5367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_115178, whole genome shotgun sequence.
ACCESSION   DATGBN010000466 DATGBN010000000
VERSION     DATGBN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115178"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VKL21_11870"
     CDS             <1..410
                     /locus_tag="VKL21_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMB46506.1"
                     /translation="TVIAATNRPDIIDPALLRPGRIDRLIYITPPDRNSRFEIFKVHT
                     AKTPLDGDVDLDTLADDTEGFTGADLASVCNEATILAIREYVVSGKSTDDESVKQLKV
                     GYHNFRDAMVKVKPHSKKELEKYTKISENFIYQ"
     gene            complement(485..718)
                     /locus_tag="VKL21_11875"
     CDS             complement(485..718)
                     /locus_tag="VKL21_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46507.1"
                     /translation="MKFVYILIVLVIAAFALGCVDKKQTETTTKTSPGETAVTATSVS
                     QAPDDLFGTESDLNAMNSTLGDMDMQISLVDAI"
     gene            complement(732..1763)
                     /locus_tag="VKL21_11880"
     CDS             complement(732..1763)
                     /locus_tag="VKL21_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46508.1"
                     /translation="MKPTNISGIFTALIMAMFILSLFSGNVAALEAPREKIVRDQIVN
                     AKEQYQINKEKNDNTREQYQKNKEKYDDTRNQFEDAKNLFEKANRRLRDAKDNKSNED
                     LNEKAREYILKAIEHTEAQLEVMKNRLDNNENLGITASDATKIIDAHTAQLEELKVKV
                     DNATTIQEIRDVHKELAGIVAKINLETRYFIGIELNRRIDNFINRADNVSVKVDDAID
                     KLDAQGKDTTKLKAEVADFKNRLEEAKDIQAKTIALFSTHSGFAADGTVTNEKDARDF
                     LKQANELQRENIKKLKEAGRQLIEFGRDFRRLVGPNVKVNDKGDLEVDGHATTTLTGG
                     ATATLTEED"
     gene            1960..2712
                     /locus_tag="VKL21_11885"
     CDS             1960..2712
                     /locus_tag="VKL21_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46509.1"
                     /translation="MFTRRGLILAIFMITGLIFSAQAAKVYGSIYDFSDFEKPLNNVI
                     VEIEENSTREQYKVSGDGTYFFNVSPGRYVIKAKFYNENNILEFSGEEKLNISQPDES
                     RNLDLILFPPIDSEFEYLGDINLTGEIETNETDYMNYIIIIPTALIIISIILFYIRKK
                     KRKGISKTDEPVPPEDKAAINISDKLPDDLRELYDIILKKGGRTTQKDLRKEVIYGEA
                     KVSLMIADLEDRGLIKKIKKGRSNIIIAENKK"
     gene            2769..3923
                     /locus_tag="VKL21_11890"
     CDS             2769..3923
                     /locus_tag="VKL21_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase family protein"
                     /protein_id="HMB46510.1"
                     /translation="MIERKEITGVLCGYDISETTIGVLASHSALDVCDGAAEEGFNTY
                     AVCEKGREKTYTQYFKAKRKNGDLVRGIVDNADVYSKFNEIMKPGNQKKMRDRNTIFV
                     PNRSFTSYCGIDEIENNFKVPMFGSRNLLRSEERGEERDYYWLLEKAGLPYPEKINNP
                     EDIDSLVMVKLPHAVKKLERGFFTAASYDEYKKKSGAFLKQGVITKESLSEARIERYI
                     IGPVFNLDMFYSPLEEKMSRIELLGIDWRFETSLDGHVRLPAPQQISLNERQLTPEYT
                     VCGHNSATLRESLLEDAFELAEKYVRATRKYYKPGIIGPFCLQTCVDKDLNFYVYDVA
                     PRVGGGTNVHVSVGHPYGNTLWRKPMSTGRRMAMEIRRGIEEERLEEIVT"
     gene            3945..5321
                     /locus_tag="VKL21_11895"
     CDS             3945..5321
                     /locus_tag="VKL21_11895"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HMB46511.1"
                     /translation="MTIKSIILAGGSGTRLWPLSREQYPKQFLKLEKTSLFQNSVLRC
                     LELSGPSEIFIVTNESQKFFVSGQMEEIGIDVPEKNILIEPHGKNTLPAICFGMNEIK
                     KQYGKCAVGVFPSDHILDKAAMEQIKSAEKLCSQYLVTFGITPVSPHTGYGYIKPGKN
                     LEIGSKVLEFREKPGLEAAKKYIKEKCLWNSGMFMFDTGVFFKELKTHAPDMLAAFTQ
                     NGKNIEEIYSELPSISIDYGIMEKSDKVAVVRLEYKWSDLGDFDSIFQETDKNEHGNA
                     SYNCDEISLNSSGNLIYSKKDKIVSLIDINNMVVVDTPDALLVCPRKSCQKVKEIVTS
                     LKNQNNEKALFHLTVYRPWGSYTIMEDSERHKIKNITVAPGKKLSLQLHNHRSEHWVV
                     VRGMAWIHVDDKEFYLRQGESTFIKAGIKHRLSNPGTIPLEIIEVQLGDYVGEDDIER
                     FEDDFGRV"
BASE COUNT         1624 a         1037 c         1142 g         1564 t
ORIGIN      
        1 ttacagtcat agcggcgacc aacaggccgg atatcattga ccctgcactt cttcgccccg
       61 gaaggattga ccgtttgata tacataacgc cacctgacag gaactcaagg tttgagatat
      121 tcaaagtcca tactgcaaag actccgcttg atggcgatgt tgatcttgat acgttagctg
      181 atgataccga aggtttcaca ggagcagacc ttgcgtcggt atgcaatgag gctacgatac
      241 ttgcaatcag ggaatatgtg gtttcaggaa aatccacaga cgatgagtcc gtaaagcaat
      301 tgaaagtggg ctaccataat ttcagggacg ccatggtaaa ggtaaaaccg cattccaaaa
      361 aggaacttga gaaatatacg aaaatatcag agaatttcat ataccagtga aaaagctctt
      421 gctcagggca aaaaataaaa aatcaaaaat gtaaacgcat acgcgtttac attaaatttt
      481 tacattaaat tgcatctacc aaggatattt gcatatccat atcgccaagt gtagaattca
      541 ttgcgttcag atcgctttct gtcccgaaaa ggtcatcagg tgcctgggac accgaagttg
      601 cagttacggc tgtttcaccg ggcgaggtct ttgttgttgt ttcagtctgc ttcttatcca
      661 cgcaacctaa agcgaaggcc gctataacca gcacgatcaa aatatataca aatttcatat
      721 tttatcctcc tctaatcttc ttcggtaaga gttgctgtgg ctccgccggt aagagttgta
      781 gtggcatggc cgtccacttc caggtcacct ttatcgttga ctttcacatt tgggccaaca
      841 agtctcctga aatccctgcc gaattctatt aactgccttc cagcttcttt taatttcttg
      901 atgttttccc tttgcagctc atttgcctgt ttgaggaaat ccctggcatc cttttcattt
      961 gtaacggtgc catctgcagc aaatccggaa tgtgttgaaa ataaagcgat tgttttcgcc
     1021 tgaatatctt ttgcttcttc taatctattc ttgaaatctg ccacttcggc cttaagtttt
     1081 gttgtgtcct taccttgcgc atctaactta tcaatagcat cgtccacttt taccgagaca
     1141 ttatctgccc ggtttatgaa attgtcgatc ctgcggttta actctattcc aatgaaataa
     1201 cgtgtttcaa gatttatttt agcaacaatt cctgcaagtt ctttatgtac gtccctgatt
     1261 tcctggatag tagttgcgtt gtcgactttt actttcaact cttcaagctg ggctgtatgg
     1321 gcatcgatga tctttgtcgc atcggaggcc gttatcccaa ggttttcatt attatcgagt
     1381 ctgttcttca tcacctcaag ctgcgcttcg gtatgttcga tcgccttgag aatatattcc
     1441 cttgcctttt cattcaggtc ttcatttgat ttattatctt tcgcgtctct taatcgcctg
     1501 ttggctttct caaagaggtt ttttgcatct tcaaattgat ttcttgtatc gtcgtacttt
     1561 tccttgtttt tctggtattg ttctcttgta ttgtcgttct tttccttgtt tatctggtat
     1621 tgttcctttg catttacaat ctggtccctg actatttttt cccggggtgc ttccaatgca
     1681 gcaacatttc ctgagaataa actcaggatg aacatcgcca taatcaaggc tgtaaatatc
     1741 cctgatatat ttgtgggttt catatttttt tcctccttct tttctaccaa tcgcattcgc
     1801 gattgtatac ccaatagtat aatgtcagaa catataaata tacttttatg tgattaaaaa
     1861 tttaagttat tttatgcttg ataattcttt ataaaacatt ataaacgctt ttttagcttt
     1921 ataaaaaact atatatacta tattttaaaa ttagaatgtg tgttcacgag aagaggatta
     1981 attctggcga tcttcatgat aaccggcctt atttttagcg cacaagctgc aaaagtatat
     2041 ggttctatat atgatttttc ggattttgag aaacctctta ataacgtaat agttgaaatc
     2101 gaggaaaatt ctaccaggga gcaatacaaa gtgtctggcg atggaacata tttctttaat
     2161 gtttcccctg gaaggtatgt tataaaagcc aaattttata atgagaataa tattcttgaa
     2221 ttttctggcg aagagaagct caatataagc caacctgatg agtcaaggaa ccttgacctt
     2281 attctttttc cacctattga ttctgaattt gaatatctcg gtgatataaa tcttacagga
     2341 gaaattgaaa caaatgaaac ggattatatg aattacatca taatcatccc gacggcttta
     2401 attattattt caattatttt attctatatc cggaagaaaa aaaggaaagg tatttcaaag
     2461 actgatgaac ctgtgccccc ggaagacaaa gctgcaataa atataagcga taaacttccg
     2521 gatgatctgc gtgaactata tgatattata ttgaaaaagg gcggaagaac aacccaaaag
     2581 gacctgagaa aggaagtgat atatggcgaa gcaaaggtca gcctcatgat tgctgacctt
     2641 gaagacaggg gtttgatcaa gaaaatcaag aagggacgtt caaacataat cattgctgaa
     2701 aacaaaaaat aaatacaatg ttttttaaga gcacaacatt catggacttt taaaacttac
     2761 aggtgaatat gatagaaagg aaggaaataa ccggagtact atgcggatat gatatcagtg
     2821 aaaccacgat aggggtactg gcatcacatt cggcgctgga cgtatgcgac ggggcggcag
     2881 aagaaggatt caatacttat gcagtatgcg agaagggacg cgaaaagacc tacacacaat
     2941 acttcaaggc caagaggaag aatggcgatc ttgttcgggg aatagtggac aacgctgatg
     3001 tttattcaaa atttaatgaa ataatgaagc ctggtaacca gaaaaagatg agggatagaa
     3061 atacgatttt cgttcccaac cgctctttta cttcctattg cgggatagat gaaattgaaa
     3121 ataatttcaa ggttccgatg ttcggaagcc gcaaccttct tcgaagtgaa gagcgaggcg
     3181 aggagcggga ttattactgg cttcttgaaa aagcaggact tccgtatcct gagaagatca
     3241 ataatcctga ggatatcgat agccttgtta tggtgaaatt acctcatgct gtcaagaaac
     3301 ttgaacgcgg gttcttcacg gcagcttctt atgatgaata caagaaaaaa tccggcgctt
     3361 tcctgaaaca gggcgttatc acaaaagaat cgctttctga agctcgaatc gagagatata
     3421 tcatcgggcc tgtttttaac cttgatatgt tctattcacc acttgaggaa aagatgagca
     3481 ggatcgaact tcttggcatc gactggcgct ttgagacaag ccttgacgga catgtgcgtc
     3541 ttcctgctcc ccagcagata tcgctcaatg agcgacagct aactcccgaa tatacggttt
     3601 gcggtcacaa ttccgcaacc ctgcgtgaat ccttactgga ggacgcattt gaacttgccg
     3661 agaaatatgt cagagcaacc cgaaaatatt ataaacccgg tataatcggg ccgttctgcc
     3721 tccagacatg tgttgacaaa gacctgaatt tctatgttta tgatgtggcg ccgcgagttg
     3781 gcgggggaac aaatgttcac gtttctgtgg gacatccgta tggtaacacg ctctggcgta
     3841 aacccatgag cacaggacgc cgaatggcaa tggaaatcag aagggggatc gaagaggaaa
     3901 gattggagga gattgtcaca taatagaaag ataaagaagg agacatgact attaaatcga
     3961 taatacttgc aggaggttca ggcactcgcc tgtggccttt gagccgtgaa caatatccca
     4021 agcagttctt gaaacttgag aagacatccc ttttccagaa ttcagtgttg cgatgtcttg
     4081 agttgtcagg gccttcagag atatttattg tcacaaatga aagccagaag ttttttgtct
     4141 caggacaaat ggaagaaatc gggattgatg tccctgagaa aaatatattg atcgaacccc
     4201 atgggaaaaa tacgcttcct gcaatatgtt tcggtatgaa tgagataaaa aagcagtacg
     4261 gtaagtgcgc tgtgggtgtt ttcccttcgg atcatatact tgataaggca gctatggaac
     4321 aaatcaagag cgcagaaaaa ttgtgttctc aatatcttgt aacctttggg atcacacctg
     4381 tatcccctca tacaggttat ggttacataa aaccagggaa aaatctggaa atcggttcga
     4441 aggttcttga attcagggaa aaacccggat tggaggctgc aaaaaaatac ataaaggaaa
     4501 aatgtctctg gaacagcggc atgttcatgt tcgacacggg ggtatttttt aaggaactta
     4561 aaacacacgc gccggatatg ctggcggctt tcacacaaaa tggtaagaat atcgaagaaa
     4621 tttacagtga acttccctcc atttccattg attatggcat tatggaaaaa tcagataaag
     4681 tggctgttgt caggcttgaa tataaatgga gcgaccttgg ggactttgat tcaatattcc
     4741 aggaaactga taaaaacgaa catgggaacg cctcctacaa ttgcgacgaa atatccttga
     4801 attcaagcgg aaatctcata tattctaaaa aagacaagat cgtatcactc atagatataa
     4861 ataatatggt tgtggttgat acacctgatg cgcttctggt ttgccccagg aaaagctgcc
     4921 agaaagtcaa ggagatcgtt acatcactta agaaccagaa taatgaaaaa gcattattcc
     4981 atctgacagt gtacaggcca tggggctcat atacgataat ggaagactcg gaaagacata
     5041 agatcaagaa tattaccgta gcaccaggca agaaactaag tcttcaattg cataatcaca
     5101 ggagtgaaca ctgggtagta gtaagaggaa tggcctggat ccatgttgat gataaggaat
     5161 tttatttacg tcagggggaa agcactttca tcaaagcggg aataaaacac aggttatcaa
     5221 atcccgggac aataccgctt gagatcatag aggtgcagct tggcgattat gtgggcgagg
     5281 atgatattga aagatttgag gatgatttcg gaagggttta attcacctca tcatctgcgg
     5341 gcttctcggc aaaggcttga tctttga
//
LOCUS       DATGBN010000467         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_502913, whole genome shotgun sequence.
ACCESSION   DATGBN010000467 DATGBN010000000
VERSION     DATGBN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502913"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(504..1862)
                     /locus_tag="VKL21_11900"
     CDS             complement(504..1862)
                     /locus_tag="VKL21_11900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMB46512.1"
                     /translation="MKFPVKDSLTEEELKSGIKNVIMDGLTTQTIATLTGGIFLVAFA
                     LKLGASNLIIGILAAIPPLGQLIQIPAIYIVEKYRIRREICIVSTAVSRSFWLLIALI
                     PFLVFIINPLNLLIIALIINAAFSAIGGYSWNSWMRDLVPVDRMGSFFGKRMIYASIV
                     GIFFSIAAGFFIDYWKQLYPDKELYAYSLIFFTGFLTGMFGLYFLSKTPEPRMVILQE
                     KINFSRLLMQPFKDTNFKNLIIFLGSWNFAVNLAVPFFTVYMLMRLKLDMSTIIILMI
                     LNQVIRIAFLHLWGKYSDRFSNKSILSINCPLFIGCILAWTFTARPDYYVLTMPLLVA
                     IHILMGISTAGITLSSGNIGLKLAPKGQATSYLAASSFVNLLAAGIAPVLSGLFADFF
                     TYRKLSLDIHWEFPVRNLDIQTLYFQYWDFLFLFAFLIGLYSIHRLALVKEVGEVESL
                     DR"
     gene            2016..2651
                     /locus_tag="VKL21_11905"
     CDS             2016..2651
                     /locus_tag="VKL21_11905"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMB46513.1"
                     /translation="MNDKEIRVSGVKELEEIGFKNKRVRIWKTMLLDYEFDNEYQDDK
                     YIWLTNVLALKAVDSGNFGVGCILVDSSDNIIAEGHNEVFNPYFRSDRHAEMVVMDKF
                     EDMNRNFAYLQDYTLYTSLESCPMCLGRLITSSAHKILHAAPDIEGGMVHESKNLPQF
                     WSDLGEGKIFSQALCSQEMINAASEIFRLNLEEFIEKIKNIQASLRLYQKN"
     gene            complement(2788..>3195)
                     /locus_tag="VKL21_11910"
     CDS             complement(2788..>3195)
                     /locus_tag="VKL21_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46514.1"
                     /translation="VTNNRDVKLETLALHYANRWLIENKFFELVDFFNLNALSSPFMI
                     RIYFDVVLTVIADTLYRLLAKDLKRFEDCTPKTIFADFINCRCIGEVVGDEVIIKMKK
                     KATTPIFKSNDVFQKSYHIPWLGNKRLRFDWIS"
BASE COUNT         1038 a          607 c          599 g          951 t
ORIGIN      
        1 ggagaaatat attgttttct ttgcaataat tccgtacaac tgtattattg aattctgtgc
       61 ccccatctga ccgtatctcg attttatcct ctttaggaat tatattcctc agatgcttta
      121 aattctggac aacaattttt gggtacaatc ccacaaaggt acaaaaccgg caatgaaaat
      181 gaggtcaagt tatagtccat gctaaacctc gttttttgag ccaattgtgt aaatttgtct
      241 atcttgtaga ttgtaagggt catgcttttt cagtgcttca aattgcgact atgagatatc
      301 cagtttgttt tttcagcttc ccctggtctt ccaagtttca ttgaagtgtt cactatttta
      361 gtgctccttt tcaacgtcct aaaacttggg agttaaaagt gctgtaaaaa tgggagacta
      421 caacccaagg aattggcgtg accgataggt atccgggtat tttcattgcc ggttttgtac
      481 ctttgtggga ttgtacccaa aggttatcgg tctaaggatt caacttctcc aacttctttt
      541 accaatgcca atctatgtat ggaataaaga ccgatcagga atgcaaaaag aaataagaaa
      601 tcccagtatt gaaaatacag ggtttgaata tcaagattcc ttaccggaaa ttcccagtga
      661 atgtccagcg aaagtttgcg gtatgtaaaa aaatctgcaa ataatccact aagcaccggt
      721 gcaattcctg ctgctaataa attaacaaaa ctgcttgctg ctaaatatga cgtagcctgt
      781 ccttttggcg ctaatttgag cccgatattt ccggatgaaa gagttattcc tgctgtggaa
      841 atacccatca ggatatgtat ggccaccaat aacggcatgg tcaatacata ataatcaggt
      901 cttgccgtga aagtccatgc aagaatgcat cctatgaata gaggacaatt tatgctgagt
      961 atcgatttat tgctgaacct gtcagaatat tttccccaca aatgcaagaa tgcaattcta
     1021 ataacctggt tcaatatcat gagaattata atagttgaca tatcaagttt taatctcata
     1081 agcatgtaaa ccgtgaaaaa aggaacggcc agatttactg caaaattcca cgatccaagg
     1141 aaaatgatca ggtttttaaa attggtatcc ttgaaaggct gcataagcaa ccttgagaaa
     1201 ttgatctttt cttgcaggat caccattcga ggttcaggtg tctttgaaag aaaataaagt
     1261 ccaaacatac ctgtcaagaa tccggtgaaa aaaatcaatg aatatgcata taactctttg
     1321 tccggataca actgtttcca gtaatcaata aaaaatccag cagcaatact gaaaaaaatt
     1381 cccactatac ttgcatagat cattcgttta ccgaaaaaag atcccatccg atccacggga
     1441 acaaggtcgc gcatccatga gttccagctg taacctccga tggctgaaaa agccgcattt
     1501 attattaagg caatgattag caggttcagg gggttaataa taaaaaccag gaaaggaatc
     1561 agagcgatca gcagccagaa tgacctgctc actgccgtac ttacgataca aatctctctt
     1621 ctgatcctgt atttctccac aatatatatt gcaggtatct ggatcagctg tccaagaggc
     1681 gggatggctg caagaatacc gataatcagg ttagaagccc caagttttag cgcaaaagcc
     1741 acaaggaata ttccacctgt caatgttgct attgtttgtg ttgtgaggcc gtccattatc
     1801 acattcttaa taccggattt caattcttct tctgttaacg aatctttaac tgggaatttc
     1861 atcacgaata ccttaattaa tgctatccca cgtcataagt cctcgtaaaa tctgcgtata
     1921 agctttatta aatgcaaata ccttcatggc gggtaaattt ttcgaatcgg acaattttaa
     1981 ataaaatata aacttgaaaa tatgggatga tccaaatgaa tgataaagaa ataagagtgt
     2041 caggggtaaa agaacttgaa gaaattggtt ttaagaataa aagggtcaga atatggaaaa
     2101 ctatgctcct ggattatgag ttcgataatg aataccagga tgataaatac atctggctta
     2161 cgaatgttct tgcattaaaa gctgtggatt ctggcaattt tggcgtgggg tgtattctgg
     2221 tagatagttc agataacatt atcgctgagg gtcacaatga agtattcaat ccctattttc
     2281 gaagtgaccg gcatgctgaa atggtggtta tggataagtt tgaagacatg aatcggaatt
     2341 tcgcatattt gcaggattac accctataca cctctttaga atcatgccca atgtgcttgg
     2401 gtaggttgat aacttccagt gcccataaga tcctgcatgc agcacctgat atagaaggcg
     2461 gtatggttca tgaatccaag aatcttcctc aattctggag cgatctggga gaaggaaaga
     2521 tcttttccca agcactatgt tctcaggaaa tgatcaatgc tgccagtgag atatttcgtc
     2581 ttaatttaga ggaattcatt gagaagataa aaaatattca agcctctctg aggctatatc
     2641 aaaaaaactg attaattata tttagaattg cccgactcgg agaataagtc cgctatgaag
     2701 actataaagg tatataaaaa tgacatcatt aaaaacagat aaacaccgat tttcgtcaag
     2761 tgtttatgtc ccataattga agtctattca tgatatccaa tcaaaacgaa gtctcttgtt
     2821 gcctaaccat ggtatgtgat acgatttctg aaaaacatca ttggacttga aaataggtgt
     2881 tgttgccttc ttcttcattt tgataataac ctcatcgcca accacttctc caatacatct
     2941 gcagttaatg aaatcagcaa agatagtctt tggtgtacaa tcctcaaatc tcttcaaatc
     3001 ctttgcaagc aacctgtata gtgtatccgc aataacggtc agaaccacat caaaatagat
     3061 tcttatcata aacggagaac tcaatgcatt gagattgaaa aaatcaacaa gctcaaagaa
     3121 tttgttttct atcaaccaac gatttgcata atgcaaagcc agagtttcca atttgacgtc
     3181 ccgattattt gtaac
//
LOCUS       DATGBN010000468         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_231689, whole genome shotgun sequence.
ACCESSION   DATGBN010000468 DATGBN010000000
VERSION     DATGBN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231689"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VKL21_11915"
     CDS             complement(<1..437)
                     /locus_tag="VKL21_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46515.1"
                     /translation="MATEGLTILGKTVFDTLFIDWRIMAITAIISIILFIVGMYIQLE
                     KWGRGIPEGATKSYGAWGALKFIISKIFEKGIGHALEILILDVAFQRRTYRRRKLEWF
                     MHILIFWGWVGLLILTIVAAVAEFAGPFLLNEDYTYFVGVWESL"
     gene            629..1894
                     /gene="cysS"
                     /locus_tag="VKL21_11920"
     CDS             629..1894
                     /gene="cysS"
                     /locus_tag="VKL21_11920"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMB46516.1"
                     /translation="MLKLFNTLTRKKEIFQPEGEVVGIYTCGPSVYQNAHIGNFRTFV
                     FEDVLVRYLKFKGYKVKRVMNLTDIEDKAIDSARKEGKTLTELTQYYSKIFFEDMETL
                     ELLPADVFPRATGHVPEIIDLIKKIMGNGYAYRGQDGSIYYNVSMFKDYGKLSHLKLK
                     KGKKKIKRDEWGEDTSIISDFALWKSYEKEDSDVFWETELGKGRPGWHIECSAMGSKH
                     LGKRFDIHVGGVDNIFPHHENVIAQNFGAFGINPSKYWLHCRHLIVDGRKMSKSAGNF
                     YTLRDLLGKGYSPMAIKYLLLSMSYRRRLNFTFEEIEEAQEKVTCFYEAIKQLNISNG
                     KDDAEKIVTKARNKFEYAMDDNLNTGKALKAMEEFTEEVKKMNPDKGSMKKILGLLSI
                     FDSILRLGLFTSAVSASHASWAEAASEAP"
     gene            complement(1833..2171)
                     /locus_tag="VKL21_11925"
     CDS             complement(1833..2171)
                     /locus_tag="VKL21_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMB46517.1"
                     /translation="MDEDLEEIKRRRLEQLQQQQYASQGQAAQQEQARAEYEAKKQAI
                     MRQILTPEARERLNTLKMTKPELVANVEAQLVSLAQSGRLASKIDDAKLKALLMQLQP
                     KKHEMQIRRM"
     gene            complement(2204..>2469)
                     /locus_tag="VKL21_11930"
     CDS             complement(2204..>2469)
                     /locus_tag="VKL21_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="40S ribosomal protein S19"
                     /protein_id="HMB46518.1"
                     /translation="YIDGPVGVSRLRSFYGGRHRNGNKPIHHAKGSGSIAREALQQLE
                     KAGFVKNQKTGRIISPAGQAFMDNSANDVKSEIIKTNPALAKY"
BASE COUNT          734 a          496 c          519 g          720 t
ORIGIN      
        1 agagattccc atacacccac aaagtaggta tagtcctcgt tcaacaggaa cggccccgca
       61 aactcagcaa ctgctgcaac aatagttaat atcaataatc ccacccagcc ccagaatatc
      121 agaatatgca tgaaccattc aagtttcctg cgcctgtatg tcctgcgctg gaatgccaca
      181 tcaagaatca agatttcaag ggcatgccca ataccttttt caaagatctt actaattatg
      241 aatttgaggg cgccccatgc accatatgat tttgttgcac cttcggggat tcctcgtccc
      301 catttttcta attgtatgta cattcccaca atgaacagga ttatggatat tatcgctgta
      361 atagccatta ttctccagtc tatgaatagt gtatcaaata ctgttttgcc taatattgta
      421 aggccttctg tcgccatatt atcctcctat tatagcctga cggctttcat tcccctaaga
      481 atcgatatca tattaagctt tcgatatgag gtcaggactc tacaatatga ggtcaggatt
      541 gagccgggaa atttttcgtg attataatag ggaggtcagg acacatgttt tattatcttg
      601 caggataaac aaatagtaaa ggtgtaaaat gctgaaactt tttaatactc ttaccaggaa
      661 aaaagaaatc tttcaacctg aaggagaagt agttggaata tacacctgtg gtccatcggt
      721 ttaccagaat gcacatattg gaaatttcag gacttttgta tttgaagatg tacttgtgag
      781 gtacttgaaa tttaagggct ataaagtaaa aagagtgatg aatcttacgg atattgagga
      841 taaggcaata gattctgcca ggaaagaagg aaagacgctt actgaattga cccaatatta
      901 ctcgaaaata ttctttgagg atatggaaac cctggaacta ttacctgccg atgttttccc
      961 aagagctacg ggacatgtgc ctgagataat cgatttaata aagaagatta tgggaaacgg
     1021 ttacgcttat cgtggacagg atggttcaat atattataat gtttccatgt ttaaagatta
     1081 tgggaaactc tctcatctaa agcttaagaa aggaaagaaa aaaataaaaa gagatgaatg
     1141 gggcgaagat acttcgatta tctcggattt tgctctatgg aaatcttatg agaaagagga
     1201 cagcgatgta ttctgggaaa ctgaactcgg aaaaggccgt cccggctggc atatagaatg
     1261 ctcggcaatg ggttctaaac atcttgggaa acgttttgat attcatgtcg ggggagtgga
     1321 taatattttc ccgcatcatg agaacgtgat agcccagaat ttcggggcat tcggtataaa
     1381 tccctcaaaa tactggcttc actgcaggca tcttatagta gatggcagga agatgtcaaa
     1441 atctgcaggt aatttctata ccctgcgcga ccttcttggg aagggataca gtcctatggc
     1501 aataaaatac ctcctactat cgatgagtta caggagaaga cttaatttca cttttgagga
     1561 aattgaagaa gcgcaagaaa aagtcacctg tttttacgaa gcgataaaac aactgaatat
     1621 atcaaatggt aaagatgatg ctgaaaagat cgtaaccaaa gcaagaaata aatttgaata
     1681 cgcaatggac gacaatttaa acacaggaaa agccctgaaa gccatggaag aattcacaga
     1741 ggaagtcaaa aagatgaatc ctgataaagg atcaatgaaa aaaatcctgg gattattaag
     1801 tatatttgat tctatactca gacttgggct ttttacatcc gccgtatctg catctcatgc
     1861 ttcttgggct gaagctgcat cagaagcgcc ttaagttttg catcatcgat ctttgaggca
     1921 agtcttccgc tctgggcaag tgacacaagc tgcgcctcaa catttgccac aagctcaggc
     1981 tttgtcatct taagagtgtt caatctctct cttgcttcag gcgtgaggat ctggcgcatg
     2041 atggcctgct ttttcgcttc gtactcagcg cgggcctgct cctgttgtgc agcctgtccc
     2101 tggctggcgt actgttgctg ctgtaattgc tcaagccttc gtctttttat ttcttctaga
     2161 tcctcatcca tactgatcaa tttccgtttt caacaaggat accttaatat ttggcaagag
     2221 ccggattggt ttttataatt tcggatttta catcatttgc tgaattatcc atgaaggcct
     2281 ggcctgccgg cgagattatc ctgcctgtct tctggttttt cacaaatccc gctttttcaa
     2341 gctgctgcaa tgcttctctt gcaatagagc cgcttccttt ggcatggtgg ataggtttat
     2401 ttccatttct gtggcgtccg ccatagaaac tacgaagtct tgaaaccccg accggtccat
     2461 cgatataga
//
LOCUS       DATGBN010000469         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_309255, whole genome shotgun sequence.
ACCESSION   DATGBN010000469 DATGBN010000000
VERSION     DATGBN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309255"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VKL21_11935"
     CDS             <1..657
                     /locus_tag="VKL21_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HMB46519.1"
                     /translation="LMGERYKVVPKEVKDYVKGLYGRTPAPVSKEIVIKVLGDEKPIT
                     CRPADLLPPELDKVTKEAQDLGIVKKPEDILTYALYPAIAPKFLRGETKEETLVAPKP
                     GNGAAVAPLPVEFKVEVDGDLFHVRIISEGGGLSVKPAESERPKVMDGAITASMQGMV
                     LKVKVKKGDQVSKGDVVLVLEAMKMENNIHAPHGGAVKDIFVKEGSTVGAGDVLMVIV
                     "
     gene            677..2161
                     /locus_tag="VKL21_11940"
     CDS             677..2161
                     /locus_tag="VKL21_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMB46520.1"
                     /translation="MFKKILIANRGEIAIRVMRACRELDVKTVAVYSEVDKNALFAKY
                     ADEAYLIGPAPANQSYLNMDKIIEVAHKTGSQGIHPGYGFLSENEKFAERCQKEGITF
                     IGPNGKTIESVGSKIAAKKTMKAAGIPVIPGSENGIGDLDEAIDVAEAIGYPIIVKAS
                     AGGGGIGMKVVRKTEELRETIESTRRVAKSSFGDATVFIEKYLEEPRHIEFQILADSY
                     GNIIHVGDRECSIQRRHQKLIEESPSPIMTPQLREKMGQDAVKAAAAINYTNAGTIEF
                     LYSKGDYYFLEMNTRLQVEHPITEIVTGVDLAKEQLRIASGEELSYKQSDIQQRGWAI
                     ECRLNAEDPLNNFTPSPGKLRRYRSSGGPGVRVDSGVHTGFTITPYYDSLISKLTVWG
                     RDRDEAIARMKRALYEYIIVGVTTNIPFHKAVMNNPNFKRGELTTHFIEDNNIIKEVE
                     KIIEAEKEKGATLASALGADEKKVATITAAVGAYIQNAKVGVAK"
     gene            2158..>3077
                     /locus_tag="VKL21_11945"
     CDS             2158..>3077
                     /locus_tag="VKL21_11945"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMB46521.1"
                     /translation="MNIKDKVLERLIKKKGMWVSGEQLASELSVSRTAIWKHISALRE
                     QGYMIDSSTHLGYSLIETPDTLTPGEIKAGLKTEIIGKDIRYFKETESTNIIALQIAA
                     RVDEGTLVIAESQTEGRGRLGRKWISPEGGIWLSMILKPKILPLHAPRITLLAGVSVA
                     RTIRSFGLPAKIKWPNDILINGKKVCGILTEIQAEADLIDYIIVGIGIDANVDTESFP
                     DEIKDSTTSLKKEKGDKINRVDFVRKLLEDFDGLYLKFQKEGIKSILEEWRNMSATIG
                     EWVKITTQTHTIYGEAIGVDNEGALILETG"
BASE COUNT          992 a          593 c          794 g          698 t
ORIGIN      
        1 ttgatgggtg aacgctataa ggtggttccg aaagaagtaa aggattatgt aaagggtcta
       61 tacggccgca cacctgctcc ggtcagcaaa gagatcgtga tcaaagtgct tggtgatgaa
      121 aaacccataa catgccgtcc tgctgatcta ttgcctccgg aacttgataa agtaacaaaa
      181 gaagcacagg atcttgggat cgtcaaaaaa cccgaagata ttcttacgta tgccctgtat
      241 cctgcgatag cccccaaatt cctgcgcggg gaaacaaagg aagaaacact tgtcgctcca
      301 aaaccgggaa atggagcagc agttgcgccg ctgcctgtgg agttcaaggt tgaagttgac
      361 ggcgatctgt tccatgtcag gatcatttcg gaaggtggcg gtttgtctgt caaacctgca
      421 gaatcggagc gcccgaaagt aatggatgga gccataactg cgagcatgca gggtatggtc
      481 cttaaagtaa aagtaaaaaa aggcgaccag gtttcaaaag gtgatgtggt gctggtactt
      541 gaagcaatga aaatggagaa taatatacat gcccctcatg gcggtgcggt aaaggatata
      601 tttgtcaagg aaggcagtac tgtaggcgca ggggatgtat tgatggtcat tgtttaattt
      661 attcaaggga tgagacatgt ttaaaaaaat ccttatagcc aaccgcggtg aaatcgcgat
      721 ccgcgtcatg cgagcatgca gggagcttga tgtaaagaca gtcgcagtgt attctgaagt
      781 ggacaagaac gcactttttg caaagtacgc ggatgaggcc tacctgatag gtcccgcgcc
      841 cgcaaaccag agctacctta atatggataa aataattgaa gtggcccaca agaccggatc
      901 ccagggcatc cacccgggat atgggttctt gtcggagaat gaaaaatttg cagaaaggtg
      961 ccaaaaagaa ggaataactt ttatcggccc caacggaaaa accatcgaaa gtgtgggaag
     1021 taaaattgcg gcaaagaaga ccatgaaagc tgccgggatc ccggtcatcc cggggagcga
     1081 gaatggtata ggcgatctgg atgaggccat tgatgtggca gaggcaattg gatatcccat
     1141 tatcgtaaaa gcctcagcgg gtgggggagg cattggcatg aaggtggtca gaaagaccga
     1201 agaactgcgt gaaaccattg aatccacccg gagagtggca aaatcatcat tcggtgatgc
     1261 aacggttttc atcgaaaaat acctggaaga gcccagacat attgaattcc agatacttgc
     1321 agattcttat ggcaacataa tccatgttgg agaccgtgaa tgttcgatcc agcgcaggca
     1381 ccagaaactg atagaagaat ccccttctcc tattatgact ccccaattgc gggaaaaaat
     1441 ggggcaggat gcagtaaaag cggctgctgc aataaattat actaatgcgg ggacaatcga
     1501 attcctatat tcaaaaggcg attattattt ccttgaaatg aacaccaggc tgcaggtaga
     1561 acatccgata acggaaatcg taacgggagt tgaccttgca aaagagcaat tgaggatagc
     1621 atcgggtgag gagttaagtt ataaacagtc ggatatccag caaagaggct gggctattga
     1681 atgccgcttg aatgcagaag atcctcttaa caactttacg ccttctcccg gaaaattgcg
     1741 aagataccgt tcttccggcg gccccggcgt ccgtgtggat agcggtgttc atacaggttt
     1801 tacaataaca ccctactacg attcgctcat ttcaaagctc actgtctggg gaagggacag
     1861 ggacgaagct atcgcccgga tgaaacgtgc attatacgaa tacatcatag ttggtgtgac
     1921 taccaatatt ccattccata aagcagtgat gaataatccc aatttcaaac gcggtgagtt
     1981 aacaacgcat ttcatagaag ataataatat cattaaagag gttgaaaaga tcatagaggc
     2041 tgaaaaggag aaaggcgcca cgcttgcatc agccctgggc gctgatgaaa aaaaggttgc
     2101 aacgatcacg gctgctgttg gcgcatatat ccagaatgca aaagtgggtg tagctaaatg
     2161 aatataaagg ataaggtact tgaaagactg atcaaaaaaa aaggcatgtg ggtttccggg
     2221 gaacagctcg caagcgagct ttcggtttca agaactgcaa tatggaaaca tatcagtgcc
     2281 ttaagggaac agggatatat gatcgattct tcaacacatc ttgggtattc acttattgaa
     2341 acacctgaca cgctgacccc gggcgaaata aaagcgggac ttaaaacaga gatcatcgga
     2401 aaagatataa gatattttaa agagacagaa agtacaaata ttattgcatt gcagatagca
     2461 gccagggtag atgagggcac gctggtaata gctgaatcac agacggaagg acgcggccgc
     2521 ctggggcgga aatggatatc accggaagga gggatatggc tttctatgat ccttaagcca
     2581 aagatactac ctttacatgc ccctcgaatt acgcttcttg caggtgtttc tgtggccagg
     2641 acgatacgaa gttttggtct tcccgcaaaa ataaagtggc ctaatgatat tctcattaat
     2701 ggaaaaaaag tatgcggaat cctcacagag atccaggctg aagctgatct tatcgattat
     2761 atcatagttg ggataggaat agatgcaaat gtagatacgg aatcattccc ggacgaaata
     2821 aaggatagta caacatcact taaaaaagaa aaaggggata agataaaccg tgttgacttt
     2881 gtaaggaagt tacttgaaga ttttgatggg ttatatctta aattccagaa ggaaggaatc
     2941 aaatccatac tggaagaatg gagaaacatg tctgcgacca taggtgaatg ggtaaagata
     3001 actacacaaa cgcatacaat atacggtgaa gcgataggtg tggataatga aggggcgcta
     3061 attcttgaga caggcga
//
LOCUS       DATGBN010000470         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_387478, whole genome shotgun sequence.
ACCESSION   DATGBN010000470 DATGBN010000000
VERSION     DATGBN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387478"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..524
                     /locus_tag="VKL21_11950"
     CDS             42..524
                     /locus_tag="VKL21_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46522.1"
                     /translation="MRRTYHLFPQNPEFERKFRFESDLPFLVSTMAGLDGQFGNFITD
                     GPDRKATPIKAKRSIEVEIAEKSVDFVPLNKNTLDSVLKDVTPDGIIDFKINLKYSVL
                     DANYNRVAFKGDTYLVRTNLDKGVLTMNIILVDGLARTDCERIADTIVEEIKIKAGNV
                     "
     gene            517..987
                     /locus_tag="VKL21_11955"
     CDS             517..987
                     /locus_tag="VKL21_11955"
                     /inference="COORDINATES: protein motif:HMM:NF021513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HMB46523.1"
                     /translation="MSDLTYTVSMPFKRKGKDAMKDTEFILALSLDLKWFNPEQAKII
                     LSEATKLGLLKREGEIVRPSFDISRIEIPSGFKPEPFSIPKKSIFDTIIDRIIVGTGI
                     EKRKVIAMINKKQEELLKQVEIEVSAILVAVENGVMVDDLIDEEYKVLTTLSSS"
     gene            complement(968..1243)
                     /locus_tag="VKL21_11960"
     CDS             complement(968..1243)
                     /locus_tag="VKL21_11960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HMB46524.1"
                     /translation="MELTILKKTDKEINIKVAGETHTLLNMLKTALLNNKHVEIATYD
                     IKHPTISEPILFVRTDGADPIDVIKKASKDMVKECNEFIDLFTKKTK"
     gene            complement(1252..>1668)
                     /locus_tag="VKL21_11965"
     CDS             complement(1252..>1668)
                     /locus_tag="VKL21_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HMB46525.1"
                     /translation="SVEPVTNTPPHLQVGDIVIGQVTDVKDSVALVEIAGIKGKGERE
                     IVNADQAAIHVSNVKDAYVKELYYEFAPFDIVKARVLDLRNMRLSTVNKELGVMKAYC
                     GNCRNVLKIDNSKLKCPKCERTETRKLSSDYGTGII"
BASE COUNT          502 a          321 c          343 g          502 t
ORIGIN      
        1 tagcaataag cttttaagat tccatgacga tacatgctgg tatgagacgc acttatcatc
       61 ttttcccaca aaacccggaa tttgaacgta aatttagatt cgaatcagac ctcccttttc
      121 ttgtcagtac aatggccgga ctggatggcc agttcgggaa cttcatcacg gatggcccgg
      181 accgcaaagc tactcccata aaagccaaga gaagcattga ggttgaaata gcggaaaaaa
      241 gcgttgattt tgtaccactc aataaaaata cacttgattc agtactgaag gatgttacac
      301 ctgacggtat aattgatttt aaaataaatc tcaagtattc cgtattggat gcaaattata
      361 accgtgtggc attcaaaggc gatacatatc ttgtaagaac aaatcttgat aaaggtgtcc
      421 tgaccatgaa cattattctt gtagacggtc ttgcgagaac cgattgtgag agaatagccg
      481 atacaatcgt cgaagagata aaaataaagg ctggaaatgt ctgatcttac ttataccgtg
      541 tccatgccct ttaaaagaaa gggtaaagat gccatgaaag acacggaatt tattcttgca
      601 ctttccctgg atttgaaatg gttcaatcct gagcaggcaa agatcatatt gagcgaagcg
      661 accaaattgg gacttttaaa acgcgaaggt gagatagtgc gtccttcatt tgatatatcc
      721 aggatcgaga taccttcggg tttcaagccg gaacctttca gtattccgaa aaaatcaata
      781 tttgacacga taatagatcg cataatagta ggcacgggga tagaaaaaag aaaagtgata
      841 gcgatgataa ataagaaaca ggaagagctt ttaaaacagg tggaaataga ggtcagcgcc
      901 attctcgttg cagttgagaa tggagtaatg gttgatgacc ttatcgatga ggaatataaa
      961 gttttaacta ctttgtcttc ttcgtaaaaa gatcaataaa ctcattacat tccttgacca
     1021 tatctttaga tgcttttttt attacatcta tggggtctgc gccgtctgtc ctcacaaaaa
     1081 ggataggttc actgatggtt ggatgcttta tatcatatgt tgctatttca acatgtttgt
     1141 tattaagcaa agctgtcttt agcatgttga gcagggtatg ggtttcgcct gctaccttaa
     1201 tattgatttc tttgtccgtc tttttaagga ttgtaagttc catgatttca cctaaataat
     1261 acctgtccca taatccgagg ataattttcg tgtctctgtt ctttcacatt tgggacattt
     1321 aagtttgctg ttatctattt tcaatacatt cctgcaattt ccgcaataag ccttcatgac
     1381 gccaagttcc ttattgacag tggagaggcg catgttacga aggtcaagga ctctggcttt
     1441 tactatatcg aacggagcga attcataata taattccttg acatatgcat cctttacatt
     1501 tgatacatga attgccgcct gatccgcatt aacgatctca cgttcacctt tgcctttaat
     1561 tcctgcgatc tcaaccaggg cgactgaatc tttcacatcg gtaacctgtc cgataacgat
     1621 atcgccaacc tggagatggg ggggtgtatt cgtgacaggc tcaactga
//
LOCUS       DATGBN010000471         8288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_231980, whole genome shotgun sequence.
ACCESSION   DATGBN010000471 DATGBN010000000
VERSION     DATGBN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231980"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..512
                     /locus_tag="VKL21_11970"
     CDS             111..512
                     /locus_tag="VKL21_11970"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HMB46526.1"
                     /translation="MISKIEPVYAKLDQENLKISGSLINLFQQHIGKTYGEIIEEIEG
                     IEELDYRLIRGLTQILERRCVIEMNSVIEPATARRSVFEECKGAVSDVKERKEVIDRV
                     AKKLSVEPDALEKALWADREDNLIIKENSRQ"
     gene            548..1279
                     /locus_tag="VKL21_11975"
     CDS             548..1279
                     /locus_tag="VKL21_11975"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HMB46527.1"
                     /translation="MTQTLLFKATGMDIQIEENYQEVFWKIKQLGLMYFIQDGRIYLD
                     GAVSLFKMTERYGTALAKLLPTIMKCNKWSLKASILKKTMQGKRIYDFTLDNTRQIFS
                     IEPDSNLETFDSAIEKEFSLLTFNSWSVKREPAVLKAGQYAFIPDFSIEKNDKRIYVE
                     IIGFWTPEYLKNKIQKINLLQEKEDLILLVDKNLACSGSEFNTDNLIFYDGKIPYLEI
                     LKILRKYEERVVTETLKGLKTLKSP"
     gene            1378..1578
                     /locus_tag="VKL21_11980"
     CDS             1378..1578
                     /locus_tag="VKL21_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46528.1"
                     /translation="MLIGDQLIDNQTLKAVKSELNGVKKHSEALIIFGNYGIKSQQIF
                     DILGYKVKWNGLDPENAEIVKI"
     gene            1626..1823
                     /locus_tag="VKL21_11985"
     CDS             1626..1823
                     /locus_tag="VKL21_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46529.1"
                     /translation="MSFNTWANSKLKNSTWVDVSLIKISAASFALMVAKLWEPLLSLD
                     WYWYAIIFVLAAIKPAYDAFK"
     gene            1855..2052
                     /locus_tag="VKL21_11990"
                     /pseudo
     CDS             1855..2052
                     /locus_tag="VKL21_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(2437..2664)
                     /locus_tag="VKL21_11995"
     CDS             complement(2437..2664)
                     /locus_tag="VKL21_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46530.1"
                     /translation="MNTNKQGLYDEFMREMGIKNRAYRTIDTIEQSLNKCQVSLDKSL
                     EDLTWDELLGYHNILLDLIPLLVIFYVICHF"
     gene            complement(2726..4426)
                     /locus_tag="VKL21_12000"
     CDS             complement(2726..4426)
                     /locus_tag="VKL21_12000"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMB46531.1"
                     /translation="MLTTEDIKILIKKYALQNAVKYNKLPQAGSVMGKVMAAPELRTR
                     AREVNSLIGEALDEINRMPPDSWEQELERLAPELIAELKEKKEPDKGLPPLNIKGRLV
                     MRFAPNPNGPPTIGSSRGIVVNSEYTKKYNGKFIIRFDDTDPATKRPMLEAYDWYLDD
                     CEWLGAKPDEVVIASDRILEYYEVAQELIKKGAAYVCFCEQEEFKALKDVKKACPHRE
                     EEVEKNLVSWKKMISGEYKEQEAVLRIKTDIAHKDPAIRDWVAFRIIKTPHPRPVVGD
                     KYNAWPLLDFEGAVEDHLLGTTLIIRGKDLADSEIRQRYVYKYMDWKYPETMHWGRVQ
                     IHEFGKLSTSGLKKAISDGIYSGWDDPRLPTIRALRRRGIRSEALRKFMIELGLGETD
                     ISLSLDTLYAENRKIVDPIANRYFFVWNPVELEVEGAEPKVAKAPFHPSRGEMREIPV
                     GTKVLVCQSDVDNLKEGERLRLKDLYNIQVTGLSPFTAKFIGTDMDIVKKEKGRIIHW
                     APPDGQKVRVLSPDGEYSGIGEQGIENDLDKVVQFERFGFVRIDSIKDDEILAYYTHK
                     "
     gene            4517..4810
                     /locus_tag="VKL21_12005"
     CDS             4517..4810
                     /locus_tag="VKL21_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HMB46532.1"
                     /translation="MQAEVSTLFGLNIYTEKGVYIGKVNDVVLEVNEKKATGLAATKL
                     NPNMFDTGSKGVVVPFRWVTAIGDVILIRHVKDQFKKPEEITEEYEIPEDEGQ"
     gene            4817..5563
                     /locus_tag="VKL21_12010"
     CDS             4817..5563
                     /locus_tag="VKL21_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HMB46533.1"
                     /translation="MIDDFSENEMKEREIYSHLRVFPPFAVRIDGRGFSRTLAGLQKP
                     YDERFAHAMADSVELFFKESGLNPLFAFTFSDEISFFFYDIPYNNRVEKIDSIIPGFV
                     SSALTIRLKLEKPVSFDSRIVLLGKEDIYKYLLWRQAETWRNHVSSYGYYTLLKSGLS
                     ENEAAIRLKNMKAKEIHELVFQKGTNLAETPVWQRCGILVYRESHEKTGYDPIKQKEV
                     KTQRKRIKQDWDTPIFKTENGRKLILKLIS"
     gene            5654..6799
                     /locus_tag="VKL21_12015"
     CDS             5654..6799
                     /locus_tag="VKL21_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46534.1"
                     /translation="MKNIKKLIISAFLIIILTNGALGYPGMGGNANNTIANNTTLPQG
                     TGNVTQIDLNNTTESDQDIIMIQHLIEVDANLLRAENKLFIRETLTFRNNGTKNFFGN
                     LRTSIPEGSENISLARSEMSQTTTGGGMPISFSQNGNIISWKDYVEQNSRLPFLYALE
                     YSVKQDADGNTEIFSKKLAIPALINYEYKEKPGLPAIIVKIIKPQGSTVKFIDENGNK
                     IAATEVDDKGEIFKFSLPQFKEIKVEISRSSAILPETQSYTAIIVIIVIGILIILVFS
                     YPYIKKKLNPDDKVSMITKKTKGQTSKYSNDKKDSSKVNNAAPSGKYKGKNAEELEDL
                     KKQFLAKIKALEKKYESGDLLDEEYEDTRNSYRDNLKEIEKMLEKMG"
     gene            complement(6801..7394)
                     /gene="pdxT"
                     /locus_tag="VKL21_12020"
     CDS             complement(6801..7394)
                     /gene="pdxT"
                     /locus_tag="VKL21_12020"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HMB46535.1"
                     /translation="MRIGVIAIQGNVEEHVNALENALIQANIKAQVIKIKHKGLIGSC
                     DALILPGGESTTLGRLLKREGIADEIKTAALAGIPIMGTCAGLVLLAKHGDEQVEKTG
                     QPLLELMDIQVNRNAFGRQRESFETLIAFKGFDEPFKAIFIRAPSITGCTNEVEILSK
                     YNDSIVAARQKNILALAFHPELTDDFRIHYFFLKMIG"
     gene            7518..7802
                     /locus_tag="VKL21_12025"
     CDS             7518..7802
                     /locus_tag="VKL21_12025"
                     /inference="COORDINATES: protein motif:HMM:NF021921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HMB46536.1"
                     /translation="MIKTQQGIHKSKGGLIRSFVSAENGKIKEISISGDFFLFPEEAF
                     FRIAEELKGIEAKREKVEQKIEEIYKRENIQSPGTLPADFTESIMKALEA"
     gene            7804..>8288
                     /locus_tag="VKL21_12030"
     CDS             7804..>8288
                     /locus_tag="VKL21_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46537.1"
                     /translation="MEEWRFIDFGIVDIRDMMAMDEAILKAGEENTFFFWTPKKSIIL
                     GFFQKANVELDLAQCKDYTITRRISGGGIAFSDDRCRQINYGVVGTIDNELFPMDIVE
                     SYKQICGVLIDTLIHYNLNAAFRPINDVVVDNRKISGNAQTRWEGKLLQNGTLLLDFD
                     I"
BASE COUNT         2663 a         1608 c         1711 g         2306 t
ORIGIN      
        1 cactaaaata aaaccatatt ccttcagcca gactttcctt acctgttttt cggcaaagtc
       61 caagtcagct atagaaatat aagttccctg atagacacta actttacggt ttgatttcaa
      121 aaattgaacc tgtttatgct aagcttgacc aggagaatct taaaatttca ggctcgctta
      181 ttaacctgtt ccaacaacat atcgggaaaa cctacgggga aattatagag gaaatcgaag
      241 gcattgaaga gctggactac cgattaatca ggggattgac tcagatactt gagagaagat
      301 gcgtcatcga aatgaattca gtcatagagc cagccactgc cagaagaagt gtttttgagg
      361 aatgcaaggg tgcagtttcc gatgtaaaag aaagaaagga ggtcatagac agggttgcca
      421 aaaagttatc tgtcgaacct gatgcactgg aaaaggctct gtgggcagat agggaagata
      481 acttaataat taaggaaaat tccagacaat aaaccctgaa gccctgctca ggcagtataa
      541 tctatccctg acacagaccc ttctttttaa agcaacgggt atggacattc aaatagagga
      601 aaattaccaa gaagtcttct ggaaaattaa gcagcttgga cttatgtatt tcattcagga
      661 tggtaggata tacctggatg gagctgtttc cttgttcaag atgaccgaaa gatacggcac
      721 ggctctggca aaactgctgc caactatcat gaagtgcaac aaatggagtt taaaagcaag
      781 catcctgaaa aaaacaatgc agggaaaaag gatttatgat ttcactctgg ataataccag
      841 gcagattttc agtattgagc ctgattccaa tttggagacc tttgatagtg ccattgaaaa
      901 ggagttctct ttgctcacct tcaatagttg gtcggtcaag agagagccag cggtattgaa
      961 agcaggtcaa tatgccttta ttccagattt ttccattgag aaaaacgaca aaaggatata
     1021 cgttgaaata atcggcttct ggacgcctga atatcttaaa aacaagattc aaaagataaa
     1081 tctgctgcag gaaaaagaag acctgatact gcttgtggat aaaaatcttg cttgttcagg
     1141 ctcggaattc aatactgaca accttatttt ctatgacggg aagataccat atctggagat
     1201 actcaagatt ctgagaaaat atgaggagag ggtggttaca gaaacgttga aaggcttaaa
     1261 aacattaaaa tcgccttaga gggaagcgtt atcaatctcg gtgatattgc cagaaaatac
     1321 gatgtcacca ttgatgccat taagacggat attaagcaga ataataggaa tggttatttg
     1381 ctcattggag accagctcat tgataatcaa acccttaaag cggttaagag cgaactgaat
     1441 ggagtgaaaa agcacagtga agctcttatt attttcggga attacggtat aaaaagtcaa
     1501 cagatatttg atattcttgg atataaggta aaatggaacg gacttgaccc agagaatgcg
     1561 gaaatagtga aaatataagg attagtaacc caatctgggc tattatatcg taatgagatg
     1621 aaaatatgag ttttaacact tgggcaaatt caaaattgaa aaatagcaca tgggtagatg
     1681 tgtctctaat aaaaattagt gctgcatcct ttgctttgat ggtagcaaaa ctatgggagc
     1741 cattgcttag tttagattgg tattggtatg caataatttt tgtgctagct gcgataaaac
     1801 cagcatatga tgcatttaaa taaggtattg tcctaatcgt ctcaatcaaa aaacatggtt
     1861 aaaaaaacat attacgaaat attagaagtc gataagaaag cctctcaaga agctataaag
     1921 tccgcctacc gcagacttgt tatgctctat catcccgaca aaaacaagtt gccagaagct
     1981 gaagagatgt ttaaaggaat cgcagaggct tatgcagtac tatcagaccc aggtaaacgg
     2041 aaacaatatg acatagacaa tgaaccgaga aatgttctgc taagagagaa acaagaacag
     2101 atgtggcaaa taataagaga gcacaaacaa agagaagcca gaacaaacca gaggctatgg
     2161 gaattccaag aacaagaacg tatcaaatat aaagaaagag aaaaagaagc tatgaatatt
     2221 atgtatgtat gaacaaaaaa aaatttaaaa aaaaaaaggt tattctttat aacgtttcgg
     2281 tatttgcaga aaggcactct ggacattttg gattagtacc ttattccttt aaattgtttc
     2341 tcacaatctt tgcaattata ttttcgtggt gctatgtttt cggtgtcaat atcaatatca
     2401 tcagaagtat cactaaaagt atcatcagaa gtaacatcaa aaatggcata tcacataaaa
     2461 tatcaccaaa agtggtatca aatccaagag tatgttgtgg taacctaaaa gttcatccca
     2521 tgttaaatct tcaagcgatt tatccaggct aacctgacat ttattcaaag attgttcaat
     2581 tgtatcaata gttctatatg cacgattttt tatgcccatt tcacgcataa actcatcata
     2641 caaaccctgt ttgttagtgt tcaatggttc cacctatgaa accaccaatg agcaccatat
     2701 tatcaataag tatgcacttt ttttgtcact tatgcgtata atacgccagg atttcatcat
     2761 ccttgatgct atcaatcctc acgaagccga acctctcaaa ttgcactact ttatccaggt
     2821 cattttcaat tccttgctcg cctatccccg aatattcgcc atcaggtgaa agaacccgca
     2881 ccttttgtcc atccggcggg gcccagtgga tgatgcgccc cttttccttc ttgactatat
     2941 ccatatcagt gccgatgaat ttagcagtaa aaggcgatag ccctgttacc tggatattat
     3001 ataggtcctt caagcgcaga cgctcgcctt ctttcaggtt atccacatcg ctctggcaga
     3061 caagcacttt tgttcccaca gggatctctc tcatctcgcc cctggaagga tgaaacggcg
     3121 cctttgcaac ttttggttct gcaccctcaa cctcaagttc aacaggattc cagacaaaaa
     3181 aatacctgtt tgctattggg tcaacgattt tcctgttctc ggcatagagc gtgtcaaggc
     3241 tgaggctaat atcggtctct ccaagaccaa gttctatcat gaatttgcga agggcctctg
     3301 atcgtatgcc gcgccgccga agcgcccgga tcgtgggtaa gcgcgggtca tcccaccctg
     3361 aatatatccc atccgatatc gcttttttca ggccgctcgt gcttaatttc ccgaactcat
     3421 gtatctgcac gcgtccccag tgcatggttt caggatactt ccaatccata tacttataga
     3481 catacctctg gcgtatctcg ctatctgcga ggtccttgcc ccggataata agcgtggtgc
     3541 ccagaaggtg gtcttcaaca gccccttcaa aatcaagaag cggccaggca ttgtatttat
     3601 cgccaacaac aggtcttgga tgcggtgttt taattattcg gaatgctacc cagtccctta
     3661 tggcagggtc cttatgagct atgtcggtct tgatccgaag cacagcttcc tgctctttgt
     3721 actcacctga gatcatcttt ttccaggata caaggttctt ttctacctcc tcttccctgt
     3781 gcggacaggc tttctttaca tcctttagcg ccttgaactc ttcctgctca cagaagcaga
     3841 cgtatgctgc gcctttctta atgagctcct gggcgacctc ataatattcc aggatcctgt
     3901 ctgaggctat gaccacctca tccggttttg cgccaagcca ttcacagtca tcaagatacc
     3961 agtcatacgc ttcaagcatg gggcgttttg tggcagggtc tgtatcgtcg aaacggataa
     4021 tgaatttacc attatatttt ttagtatatt ccgagttcac aacaataccc cgtgaactac
     4081 ctatggtggg cgggccattg ggatttggcg cgaacctcat cacaagacgg ccctttatat
     4141 taagcggggg aaggccttta tccggctctt tcttttcctt aagttctgcg atgagttcag
     4201 gtgcaagccg ctccaattcc tgttcccacg aatcaggggg catcctgttt atctcatcca
     4261 gcgcttctcc tattagcgaa tttacttccc tggcccttgt ccggagttcc ggtgctgcca
     4321 tgaccttgcc catgaccgaa ccggcctgag gcagcttatt atactttact gcattttgca
     4381 gtgcatattt ctttataagt attttaatat cttcggttgt taacattgga acctaaatca
     4441 tcatatggga atattaaact tctgcgaatc aattaagttt atattgatat cattcatatt
     4501 tagaggaggt gatattatgc aggctgaggt ttcaacacta ttcggattaa atatttacac
     4561 agaaaaagga gtctatattg gaaaagtgaa cgatgtcgta cttgaagtaa atgagaagaa
     4621 agcaacaggt cttgctgcaa caaaattgaa tcccaatatg ttcgatacag gcagcaaagg
     4681 ggttgtggtc cctttcaggt gggttacggc aattggtgat gttatcctta tcagacatgt
     4741 caaagaccag ttcaagaaac ccgaagagat cacggaagag tatgaaattc cggaggatga
     4801 gggacagtaa taaatcttga ttgatgattt ttcagaaaat gaaatgaaag agagggaaat
     4861 atattcccac ctcagggtgt tccctccttt tgcggtaagg attgatggcc ggggttttag
     4921 tcgcacgctt gcggggcttc aaaaaccgta tgatgaaaga tttgcccatg caatggcgga
     4981 ttccgtagaa ttatttttca aggaaagcgg cctgaatccc ctgttcgctt ttacattctc
     5041 ggatgagatc tccttctttt tctatgatat cccttataat aacagggtcg agaaaatcga
     5101 ttccattatc ccgggatttg tgtcctcggc gctaactatc cggttgaagc ttgaaaaacc
     5161 agtttccttt gattcaagga tcgtccttct gggaaaagag gatatttata aatatctttt
     5221 atggagacag gccgagacct ggagaaatca tgttagttct tatggatatt acacgctatt
     5281 aaaatccgga ttgagcgaaa atgaggctgc aatacgcctt aaaaatatga aagcaaaaga
     5341 aatccacgag cttgtattcc aaaagggcac aaatcttgct gaaacacccg tgtggcaaag
     5401 atgcggaata ctggtttaca gggaatcgca tgaaaaaaca ggttatgatc caataaaaca
     5461 aaaggaagtc aaaacgcaaa ggaaaagaat aaaacaggat tgggatactc ctattttcaa
     5521 gactgagaat gggcggaaac ttatattaaa gcttatatca tgattcgtcc tatggatttc
     5581 ggattaaatc gtggtagatc aaatagataa cttaattatt atcttaaata caatagtgac
     5641 tggaagtgaa tcaatgaaaa acataaaaaa attaataata tcagcatttt tgatcattat
     5701 attgacaaat ggcgcgttgg gatatccagg aatgggcggt aatgccaata atacgattgc
     5761 caataatacg accttgccgc aaggaactgg taatgttaca caaatagacc ttaacaacac
     5821 tacagaatca gatcaggata tcattatgat acagcatctt attgaagtgg atgcaaacct
     5881 gttgagggca gaaaataaat tgttcatccg tgaaactctt actttcagga ataacggtac
     5941 aaagaatttc tttggaaatc tgcggacatc gatacctgaa ggttcggaaa atatcagtct
     6001 tgcccggtcg gaaatgtcgc aaacgacgac aggcggcggg atgccaatct ctttttcgca
     6061 aaatgggaat attataagct ggaaggatta tgttgaacaa aacagccgtt taccgttcct
     6121 ttatgcttta gaatatagtg ttaaacagga tgctgatgga aatactgaaa ttttttcaaa
     6181 gaaattagca attcctgcat taataaatta tgaatataag gaaaaaccgg gccttccggc
     6241 tattattgta aagatcatca aacctcaagg aagcacggtc aaattcattg atgaaaatgg
     6301 caacaagata gctgcaacag aggtggacga taaaggtgag attttcaagt tttcattacc
     6361 gcaatttaag gaaataaaag tcgaaatatc acgatccagc gcaattttgc ctgaaactca
     6421 aagctatacc gcaattatcg taattatcgt tattggaata ttgataatac ttgttttttc
     6481 gtatccgtat ataaagaaaa aattgaaccc tgatgataaa gtaagcatga taaccaaaaa
     6541 aactaaagga caaaccagta aatattctaa tgataaaaaa gactcttcaa aggttaacaa
     6601 tgcggctcca tcgggaaaat ataaaggaaa aaatgcagaa gaacttgaag atctcaagaa
     6661 acaattcctg gcaaaaataa aggcacttga aaagaaatat gaatcagggg atcttcttga
     6721 tgaagaatat gaggatacaa ggaactcata tcgggacaac ctgaaggaaa ttgagaagat
     6781 gttggaaaag atgggatgat ctatcctatc attttcaaga aaaaatagtg tatccggaaa
     6841 tcatccgtca attcaggatg aaatgcaaga gccagaatat ttttctgtct tgctgccaca
     6901 attgagtcat tatactttga aaggatctcc acttcatttg tgcatcctgt tatgctgggc
     6961 gcccgaataa atatcgcttt aaaaggttcg tcaaatcctt taaaagcgat cagtgtttca
     7021 aatgattctc tctggcgccc gaaagcattc ctgtttactt gaatatccat cagttccagc
     7081 aatggctgcc ctgtcttttc tacctgttca tcgccatgtt ttgcaagaag aacaagcccc
     7141 gcacatgttc ccataatggg aattccggca agtgcggctg tctttatttc atcagcgatg
     7201 ccttcccgtt tcaaaagacg gccaagtgtg gtactttctc ccccgggtag tatcaaagca
     7261 tcgcatgaac caattaatcc tttgtgtttt atttttatta cttgagcttt aatattagct
     7321 tgaattagcg cattttctaa tgcattgaca tgttcctcga catttccctg gatagcgata
     7381 actccgatac gcatgcatct aacttaatat tttagaatat gaacctaccg atagtttgtt
     7441 ttctatcata caaccagaac gaaaaagata ttttcttgta gaagaatgat gttcctggaa
     7501 tagccggagc aaatacaatg ataaaaacgc aacaggggat ccataaatcg aaaggcggac
     7561 taataagatc attcgttagt gccgagaatg gaaaaattaa ggaaattagc atctcaggag
     7621 atttcttttt atttcctgaa gaggccttct tcagaattgc agaagaactt aagggaattg
     7681 aagcaaaacg tgaaaaagtg gaacagaaga tcgaagaaat atataagaga gaaaacatcc
     7741 aatcccccgg tacattaccg gctgatttca cggaatccat aatgaaagcc ctggaggcat
     7801 aaaatggaag aatggcgctt tattgatttc gggatcgtag atattcgaga tatgatggct
     7861 atggatgaag ctatcctgaa ggcgggcgaa gaaaatactt tctttttctg gacgcctaag
     7921 aaatccataa tactgggttt tttccagaaa gcaaatgttg aactggatct cgcgcagtgt
     7981 aaagattata cgataacaag gcgcataagc ggtggaggca ttgcattttc agatgacagg
     8041 tgcaggcaga taaattatgg cgttgtgggg actatcgata acgaactctt tcccatggac
     8101 atcgtagaat catataaaca aatatgtggc gtccttattg atacactaat acattataac
     8161 ctgaatgcag cttttcgccc tataaacgat gtagttgtag acaacaggaa gatctcaggg
     8221 aacgcacaga cgcgctggga agggaaactg ctgcaaaatg gaacgctact tcttgatttt
     8281 gatataga
//
LOCUS       DATGBN010000472         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_115799, whole genome shotgun sequence.
ACCESSION   DATGBN010000472 DATGBN010000000
VERSION     DATGBN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115799"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..573)
                     /locus_tag="VKL21_12035"
     CDS             complement(181..573)
                     /locus_tag="VKL21_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMB46538.1"
                     /translation="MSESDLVEIEDTTWEQLVEKSSKPVIVMFYSLACPFCEAMEPYF
                     ENFAQEFKNSAVFARMNVITNPWTAERYGVQGTPTFKFFCHGRPVWEQVGEIYPFILE
                     TAIKNMVNYGEDCIRKSTPVGQDVTGYV"
     gene            complement(846..1316)
                     /gene="bfr"
                     /locus_tag="VKL21_12040"
     CDS             complement(846..1316)
                     /gene="bfr"
                     /locus_tag="VKL21_12040"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HMB46539.1"
                     /translation="MKGNEKIIEHLNARLADELTAINQYMVHAEMCENWNYKQLDEMI
                     QKRAIAEMKHAEKLIARILFLEGKPIVSNLNQITIGAEVPKMHENDHGAEEGAIRGYN
                     ESIRLAAEVGDNNTKVLLESILKEEEEHIDWIEAQLDQIKQMGIQNYLVEQIYE"
     gene            complement(1364..1720)
                     /locus_tag="VKL21_12045"
     CDS             complement(1364..1720)
                     /locus_tag="VKL21_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMB46540.1"
                     /translation="MPMKEWGSGKIFTPAVDIKEEEDKLVVTTDLPGINKEDIEINLK
                     EDMLEISAKSRKEKETEEEGYIRKEREYTRFYRAVRLPTSVKEEESTAKIENGVLTMT
                     LPKMKLEDPVKKIRIE"
BASE COUNT          652 a          506 c          430 g          786 t
ORIGIN      
        1 gaggatggaa ttagaagcca ttcccaaagt gaaaacttgg agttattttt taaccattcc
       61 ttagcatcct gactgcaact aggctatttt tggtgaattt atgcttgtaa taaaggaaat
      121 atacagatga aaaggtaaga gaaaaaagga aagatgacat aagagccaat ggcctgcagg
      181 tcaaacatat cctgtaacat cctgtcctac tggagtgctt tttcttatac aatcttctcc
      241 ataattgacc atgttcttaa tcgcagtttc caatatgaaa ggatatattt cacctacctg
      301 ttcccataca ggtctaccgt gacagaaaaa tttgaatgtg ggcgtaccct gaactccata
      361 cctttctgca gtccagggat ttgttataac attcatgcgg gcaaagaccg ctgagttctt
      421 gaattcctgt gcaaaatttt caaaataagg ttccatagcc tcacaaaaag gacatgccag
      481 gctgtaaaac attacaataa ctggttttga agatttttct accagctgtt cccatgttgt
      541 atcctcaatt tcaaccagat cactttcgct caatcactta cccccaggat attgtattaa
      601 ccgaatatct ggctgcaggc atctctcacc tgcagctcat aatatcaact tttctggcat
      661 ctactgccgt taatgaccac aaggtctcga ggatttcgtg aggacagcaa ctgaaggatt
      721 ccagaaaggt cttcaagatc cacgctagta catggcctgt attcaagcct atgataatac
      781 atgaaaatga taatgctttt ataccggcct tcctcaaaag tgcttagata cagtaggcat
      841 cgccctcatt cgtagatctg ctccaccaga tagttctgga tacccatctg tttgatctgg
      901 tcgagttgag cttcaatcca gtcgatgtgc tcctcttctt ccttcaggat agactctaga
      961 agcactttgg tgttgttatc tcccacctct gccgccagac ggatgctttc gttatatcct
     1021 cggatggccc cctcttcagc tccgtggtca ttctcatgca tcttcggcac ttcggcgccg
     1081 atggtaatct ggttcaaatt actgacgatg ggtttccctt cgaggaaaag aatgcgggcg
     1141 atcagcttct ccgcatgctt catctcggcg atggctcgct tctgtatcat ttcgtccagc
     1201 tgcttgtaat tccagttttc gcacatctcg gcatggacca tgtactggtt gatagcggtc
     1261 agttcatcgg ccaaacgagc gtttagatgt tctatgatct tctcatttcc cttcatacat
     1321 aaacccccat tacatcgaaa attatctttt tgttctcgaa tgattattcg atccgaattt
     1381 tctttactgg atcttcaagt ttcattttcg gaagcgtcat tgtcaagaca ccattttcta
     1441 tttttgcagt gctttcttcc tctttaacgc tggtaggcaa gcggacagcc cggtaaaagc
     1501 gagtatattc cctttcttta cgaatgtatc cttcctcttc ggtttctttt tcctttctgc
     1561 tctttgcact tatctcaagc atgtcttctt taaggtttat ctcaatatcc tctttattga
     1621 ttcctggcaa gtcggtagtg acaacaagct tgtcttcttc ttccttgatg tcaacggcag
     1681 gagtaaatat ctttccgctt ccccattctt tcataggcat gaaatcttca tacaactggt
     1741 tcaggcgctc ctgcgtcctc ctaatctctt caaaaggatc ccagcgtgtg ggttctgaaa
     1801 ctgttttctt agcaggccat ttaatctaaa attcccccta aagtaaaatt ataacacaag
     1861 atttgtgtta ttttgaatat attgtgggag gctatatagt gttttcttta acaatttcgc
     1921 taaatgtagc acaaaaagga accaaacaga taggtcgttc cgtgagttgt tgaaaatcgt
     1981 aaacatgaga cactttctac attttctcaa caatgggggt ataatctttt tccagatttg
     2041 attaattatt caagaacaca actcaatatt taggttattt ttaatttttt cacacagtgt
     2101 gtataatagt tagtaccttg ccttcaaaac tcagaatttg atataaaatt agtctgtgtt
     2161 gcttttttag gaaaaaaacc caatgctaaa ttttgaagat tccagtaaat aatataaaac
     2221 ataattaata ttaatttgtt gttcgatttt aaaacatttt atgtggaagt aacttttgtt
     2281 cagaaaccat ttttcgtata attaatttaa aataacaatg gggataattg tgtattgtaa
     2341 gggagcaaat aggtcaaaag agtgaggaat aagg
//
LOCUS       DATGBN010000473        10273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_426286, whole genome shotgun sequence.
ACCESSION   DATGBN010000473 DATGBN010000000
VERSION     DATGBN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426286"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2614
                     /locus_tag="VKL21_12050"
     CDS             <1..2614
                     /locus_tag="VKL21_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46541.1"
                     /translation="FLRDDSARVPFVVIGIFFVILSTLVSLNLTRMDIKMAKTMSANP
                     EISAPDQALSYAKADLARALNYAGMEALKSLGETPVMIPDNTSEYNSGPYQFNMNWAL
                     AMTNHTFNQYMESNYMYDTFTYSGYSVNVEPTYSWKEMTITPIRMKLNRTINPPLFEP
                     GDNGYETYWKLSMPVRIHLKNLDMKTELLSENISVETLITSRYPLLRDLTQEYGKRVN
                     GTNAVMTETTAFAMAYTWGRGYMQYGKGTPVNIVNNSNLALIINGALLLDQGFVFNSI
                     DPKSLVEYANQTAATLSGKKKKYEDVVLDNNSLKVDPREDAYNSTADPEKAREESQNG
                     KYDFNITIITDYLNNNSKQGGSIVNREIKTVIPQVYSASFATGVARVVFENPGDHSGY
                     EESYSIDAWGEPDSMTQTGTIPGDSYVPGNLYGETWELTWTRNHVWRHTYWVTESCGE
                     KCTMQVPHYNYMTAQDSRMDKVAITLKAIENSNTNIDLDFAGRSLASKNDLYGTFNPE
                     DVTYRFEHNDPNLEAAYLQYRDFYDAAKIPNLKNLDLNGDTGAKSYTLDAPAWVNEEV
                     QFAVDDITGMMKNDIHLDPGINYENYPVPSDLINAVRDDLIKKISEKEVTYANKSGYF
                     SGKYSSASAKAISVIREWYVDQVKYQVREKFSQGSETIEDEIKKNFSEPEKIKQANRD
                     AGTFLSRGFKLPLAVSMRAFHVDENGNIYPPQKLQAWNESVTLLINQEPNYLDAQMPY
                     GEEQLYTLKLRNVNLLGVTGVHILPSLEPWIATFNMWSIDVEGEFVKFEVQDVDNEVH
                     PDPIFGHEAQVYVRKYETVKDSISNSFIGENSRMKFNFTTGTFILVPPGKLEGVGDKD
                     WEIVEESPGFKVKK"
     gene            2611..3204
                     /locus_tag="VKL21_12055"
     CDS             2611..3204
                     /locus_tag="VKL21_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcinarray family MAST domain-containing
                     protein"
                     /protein_id="HMB46542.1"
                     /translation="MKFRTLVFIFIILFYNFQPTMGAENEYGIGKAWLNGKNATVNGI
                     QLKIGEPFEVKVELLSKIKGDVSFKLYEPGVTKAFKVLIGPSEIEKRIDIMNIESGWL
                     KTYSWTIAPNGEWKGGNAPLNVIIQFNKGMINKQIDFTIANPYILDEQYSGAVPTPET
                     TASPVGTQAKATPFLSVIFMVGALLLVWIWRREKKVK"
     gene            complement(3244..6069)
                     /gene="uvrA"
                     /locus_tag="VKL21_12060"
     CDS             complement(3244..6069)
                     /gene="uvrA"
                     /locus_tag="VKL21_12060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMB46543.1"
                     /translation="MSIDKIIIKGARVHNLKNIDLELPRNKLIVITGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVEYIEGLSPAISIEQKSTSKNPRSTVGT
                     VTEIYDYLRLLYARIGKQHCPNCKSEITPQTVEQIVSRLMSLPESTKLHILAPNARQK
                     KGEYKQIFEGFSQSGYSRVRIDGNIHELTREITLNKQQKHDIDVIIDRLIIKKGIEER
                     LADSVATALREGNGIAVAQALEGKEFLFSEHAACVKCGISFEPLEPNMFSFNSPRGAC
                     PSCQGLGNTMEFDPDLIIPDKNKSISEGAIEPWGYEHGYYRQMLQSVAKHYDFSLDAP
                     FSELKPEHVQIILNGSIEAILFKYVPRDRDGLWEHRSVFEGIIPHLKRKYRESQSEEA
                     REKIQQYMSINPCTVCNGERLKPSSLAVTIMDRSIITTTRLSVKKGLEFLEGITFSEK
                     EMIISKPIMKELRSRLGFLMDVGLDYLTLDRASGTLSGGESQRIRLATQIGSSLVGVL
                     YILDEPSIGLHQRDNGRLINMLTRLRDLGNTVIVVEHDEEMIRSADFIVDMGPGAGVH
                     GGEIVMTGSLEDIINCDNSLTGDYLSHRKYINVPSHRRKPKGKITIVGAGENNLKQID
                     VNIPIGVMTCVTGVSGSGKSTLVNDILYRVLAQKFYHSKEKPGKHRGIIGFENIDKII
                     IIDQSPIGRTPRSNPATYTGLFTPIRDIFAKLPSSKARGYQPGRFSFNLKGGRCEACS
                     GDGIITIEMHFLPDVYVPCEICHGKRFNSETLEITFKGKNIAQVLDMTVEEALTFFEN
                     IPKIKRQLETLFDVGLGYIRLGQPATTLSGGEAQRIKLSTELSKRSTGRTLYILDEPT
                     TGLHFADIQRLLDMLSRLVDAGNSVVVIEHNLDVIKVADHIIDLGPEGGDNGGRIIAE
                     GTPETVAKIEGSYTGYYLKKALNRERG"
     gene            6183..7061
                     /locus_tag="VKL21_12065"
     CDS             6183..7061
                     /locus_tag="VKL21_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46544.1"
                     /translation="MIIAVILLMLPIILLWVTGNSALDKFGITKIYETVFGGREWTSQ
                     WDNGHARTWTDSSNDPYDAEFVTQDKGTGSYKADGLGTLKISGDGPRMYVLDSNKIKN
                     WHNVEITLYGRRVSDDNTTWGGIMAYARTNHLIDSDYCDTRGYGGRFRYDGDIDFEKE
                     TSHPDSVAVSNKVNPGGFPKNVWIGYKYVVYDMSDGNIKLELYRDNTDGVNGGTWVKI
                     NEFIDTGSNFGVAGTSCKSGINPFLRLTASDTRPGSETGKPNLDIYFRSDGVGSDGLW
                     YKKASIREIVPIRTGS"
     gene            7337..8236
                     /locus_tag="VKL21_12070"
     CDS             7337..8236
                     /locus_tag="VKL21_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMB46545.1"
                     /translation="MKYRNSTNLIYIKYMGEKSMNINILGSGVVGAATGKGLKNIGNN
                     NVLFYDIDTNRIESLKEQALNATTDLDHAVKSSEIFIICVPTPDKNKMIDLSIIESAV
                     KELSKECKDKKDYFLIVIKSTVIPMTTENLIIPLIEKYSNKKVGNDFGVCFNPEFLRE
                     ANPYEDFMNPDRIVIGEYDKKSGDILEQVYSPFKCPIIRTNLRTAEFAKYANNCFYAT
                     KISFFNEIHLMCKHIGIDSNIIRKVVQMDKYYGIHPWEHGHSFGGKCLPKDLNAVIGL
                     FNEGHIHDPILLKAVRKINEIIE"
     gene            8439..9551
                     /locus_tag="VKL21_12075"
     CDS             8439..9551
                     /locus_tag="VKL21_12075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMB46546.1"
                     /translation="MSRITGSFFYNYDNKWKDNNYEPAVSFVIPVKNEENVISNTIAK
                     CYEVDYPKDKIEVIVINDGSTDNTLKEIENAKKIYKDLTIIDWKINKGKRYGMAEGFK
                     IAKGEIVIQLDSDSYIEKSSLKTLIRPFQDGDIGAVAAHSDPQNANENFLTKMQAAYY
                     FISFRAMKASESLFDTVFCCSGCCSAYRKNIVLPVLDKWINETFLSKPVSFGDDRSLT
                     NQLLKAGHKTIYLNKAQAYTIVPNNIRQFIKQQIRWKKSWVINAIFLSQFIFKRNKFV
                     AITYFIPVIIISLFTPFIAFKAIILNPVIFGISPIFYIIGVFLVSSLLAIHYMFFRGD
                     KNYKYIYAWIFLGLVFTSYLMFYALLDIRNMKWGTR"
     gene            9557..>10273
                     /locus_tag="VKL21_12080"
     CDS             9557..>10273
                     /locus_tag="VKL21_12080"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HMB46547.1"
                     /translation="MKIKDNKFLNNNRIKLYFSLMILVWIILIISSPFIMERVVSAIN
                     PNTYSRIPLIGYYQQSNVNSLNTLIDINDNLSCQPKFPIEKTIILRMDDVQKSAYDDL
                     VINLTETVLSKNMSITLGVIPKNINDDSIIKTYLIGKMNDPRIEIAQHGTNHTFLEFL
                     PNITKQEVYDMTKPGLDQIIMALNVYPVTFIPPNNAYNEKAPGAIKELGFKILSAKKG
                     EFKFSDGILYAGFDIATKYSS"
BASE COUNT         3210 a         1952 c         2173 g         2938 t
ORIGIN      
        1 tttcctcagg gacgactctg cccgcgtccc ctttgttgtg atcggaatat ttttcgtgat
       61 cctctccacg cttgtttccc tgaatcttac ccgcatggat ataaaaatgg caaagacaat
      121 gtcggcaaat ccggaaatat cagccccgga ccaggcatta tcctatgcaa aggcagacct
      181 tgcaagagcg cttaactatg cgggaatgga agcgctaaag tcgctcggtg agactccggt
      241 catgataccg gataacacaa gtgaatacaa cagcggccct taccaattca acatgaattg
      301 ggcgctggcc atgacaaatc atacattcaa ccagtacatg gaatcaaact acatgtacga
      361 cactttcaca tacagcggat attccgtaaa cgtagaaccc acgtattcct ggaaggaaat
      421 gaccatcacg ccaataagaa tgaaactcaa caggactata aacccgccgc tctttgagcc
      481 tggagacaac ggttatgaaa catactggaa actatccatg cccgtcagga tccatcttaa
      541 aaaccttgac atgaaaacag agctccttag cgagaacata agcgtagaaa ctttgatcac
      601 atcacggtat ccgcttttgc gtgacctgac ccaggaatac ggcaagagag taaatggcac
      661 aaatgccgtg atgacagaga caactgcgtt tgccatggca tacacatggg gccggggata
      721 tatgcaatat ggaaaaggaa cacccgttaa catagtgaac aactcgaatc tcgcgctgat
      781 catcaacggc gcgctgcttc ttgaccaagg gtttgtgttc aattcaatcg accccaaaag
      841 ccttgtggaa tatgcaaacc agaccgctgc aacgctttca ggaaagaaga agaaatacga
      901 agatgtggta cttgataaca attcccttaa agttgatccc agggaggacg cgtataattc
      961 cactgctgat cctgaaaagg cccgggagga atcacagaat ggaaaatatg atttcaatat
     1021 taccataatc acggattatc tcaacaataa ttcaaagcag gggggttcta ttgtaaaccg
     1081 ggaaataaag acagtgatcc cgcaggtata cagcgcttca tttgcgacag gcgttgcaag
     1141 ggtggttttt gaaaatccgg gggatcacag cggttatgaa gaaagttaca gtattgatgc
     1201 ctggggcgag cctgacagca tgacccaaac aggaactata ccaggagatt cgtatgttcc
     1261 cggtaatctg tatggtgaaa catgggagct gacctggacg cgaaaccatg tatggcggca
     1321 tacttactgg gtcacggaat catgcgggga aaagtgcacg atgcaggtgc cgcattataa
     1381 ttatatgacg gcacaggatt cgaggatgga taaggtggca attaccctga aggcgattga
     1441 gaattcgaat acgaatattg atcttgattt tgcaggtaga tctcttgctt cgaaaaatga
     1501 cctgtatgga acatttaatc cagaggatgt gacttacagg ttcgagcata atgacccgaa
     1561 tcttgaagct gcgtacctgc aatacaggga tttttatgat gctgctaaaa tcccgaacct
     1621 gaagaacctc gacctgaatg gcgatactgg cgcgaaatcc tacacactgg atgcacccgc
     1681 atgggtcaat gaggaggtgc agttcgctgt tgatgatatt accggaatga tgaaaaatga
     1741 tattcatctt gaccccggga tcaattatga gaattatccc gttccttcgg acctgatcaa
     1801 tgcggtgcgg gatgacctga ttaagaagat ctcggaaaaa gaggtcacgt atgcgaataa
     1861 gtccggttat ttttcaggca agtacagcag cgcgagcgca aaggcaatat ctgtcattcg
     1921 cgagtggtat gtggatcagg tgaagtacca ggtacgagag aaattctctc agggaagcga
     1981 aacgatcgag gatgagatca agaagaactt ctccgaacct gagaagatca agcaggccaa
     2041 cagagatgca ggaaccttcc tgtcccgcgg gttcaaactg cctcttgctg tgagtatgag
     2101 ggcatttcat gtggatgaga atggaaatat ttatccgcct cagaagctac aggcatggaa
     2161 tgagagtgtg acgctgttga tcaaccagga gccaaattat ctggatgcgc agatgcctta
     2221 cggtgaagag cagctttata cattaaaatt acgtaatgtc aatctgcttg gtgtgaccgg
     2281 agttcatatt ttgccgagtc ttgaaccctg gatagcgact ttcaatatgt ggtcgattga
     2341 tgtggaagga gagttcgtga agtttgaggt gcaggatgta gataatgaag tgcatccgga
     2401 tccgattttc gggcatgagg cgcaggtgta tgtgagaaaa tatgagactg ttaaggattc
     2461 aatatctaat tctttcattg gagaaaattc tcgcatgaaa ttcaatttta caacaggaac
     2521 tttcatatta gtacctccag gcaaactgga aggagtggga gataaagatt gggagattgt
     2581 cgaagaatct ccaggattta aggtgaaaaa atgaaattta gaactttagt atttattttt
     2641 ataatattat tttataattt tcaaccaaca atgggtgctg aaaatgaata tggtataggt
     2701 aaagcttggc ttaatgggaa aaatgctacg gtaaatggca ttcaattaaa aattggcgaa
     2761 ccgtttgaag tgaaagtgga attattatct aaaatcaaag gtgatgtttc atttaagtta
     2821 tatgaaccag gtgtaacaaa ggcctttaaa gtattaattg gaccaagtga aattgaaaag
     2881 cgaattgata ttatgaatat agaatctggg tggctaaaaa catatagttg gacaatagcg
     2941 ccaaacggtg agtggaaagg aggaaatgcc cctttaaatg tgataatcca atttaataaa
     3001 ggaatgatta ataaacaaat agatttcacc atcgccaacc cttacatcct cgacgagcaa
     3061 tattccggcg ccgtccccac gcctgaaacg accgcctccc cggtgggaac tcaggcgaag
     3121 gcgacgccgt tcctttcagt aatattcatg gtcggtgcac tattgctcgt atggatatgg
     3181 agaagagaaa aaaaagtgaa atagcacacg gatgacgctg attacgcgga tttgcgcgga
     3241 taatcatccg cgttccctgt tcaaagcctt cttcaaataa taccccgtgt acgatccctc
     3301 tatctttgcc accgtctcag gcgttccctc ggcaatgatc cgtcccccgt tatccccgcc
     3361 ttccggccca agatcaatta tatgatcggc aacttttatt acatcaagat tgtgttcgat
     3421 aaccaccacg ctgttgccag catccactaa cctggaaagc atatcaagaa gcctttgtat
     3481 atctgcaaaa tgaagaccgg tggtaggctc atccagtatg tagagcgtcc ttccagtact
     3541 tcgtttactg agttccgttg aaagcttgat ccgctgcgcc tcaccgcctg aaagagtagt
     3601 tgcgggctga ccgagcctga tatagcccag ccccacatca aaaagtgtct caagctgccg
     3661 ttttattttg gggatgtttt caaagaatgt cagagcttct tcaacggtca tatccagcac
     3721 ctgtgcgata ttctttccct tgaatgtgat ctcaagcgtc tcgctgttga aacgctttcc
     3781 atggcagatt tcgcatggga catagacatc aggaaggaaa tgcatctcta ttgtgataat
     3841 cccgtccccg ctgcatgctt cacatcgtcc tcccttcagg ttgaagctga aacgcccggg
     3901 ctggtaaccc cgcgctttcg atgagggaag ttttgcaaaa atatctctta tgggcgtaaa
     3961 aagtccggta taggttgcag gattggaacg cggtgtcctt cctatgggtg actggtcgat
     4021 tattatgatc ttatcgatat tctcaaaacc gattattccc ctgtgttttc cgggtttttc
     4081 ttttgaatga tagaactttt gcgcaaggac cctgtagaga atatcattga caagagtgct
     4141 ctttcccgaa cctgaaacac ctgtgacgca tgtcatgacc ccgataggaa tattaacatc
     4201 tatttgtttc aggttatttt cgcctgctcc cacaattgtt attttgcctt ttggttttct
     4261 tcggtgagag gggacattta tgtatttgcg gtggctgagg taatcacccg taagggaatt
     4321 atcacaattg attatatcct caaggcttcc ggtcatcacg atttcgccgc catgaactcc
     4381 tgccccgggc cccatatcca cgataaaatc cgcgcttcgt atcatttctt catcgtgctc
     4441 taccacgatc accgtatttc caaggtcgcg aagccgggtg agcatattta taagcctccc
     4501 gttatccctc tggtggagac caatacttgg ttcatcaagg atataaagaa cgcctacaag
     4561 gcttgagccg atttgtgtgg caagccggat cctctggctt tcacctcccg aaagagttcc
     4621 tgatgccctg tcaagtgtca ggtaatcaag accaacatcc attaagaatc caaggcgaga
     4681 acgcagctct ttcattatgg gcttcgatat tatcatctct ttttcagaaa aagttatccc
     4741 ctcaagaaat tcaagacctt ttttcactga cagccgggta gtggtaatta tggatctatc
     4801 catgatcgtg accgcaagac ttgatggttt taacctttca ccattacata ccgtacaggg
     4861 gttgatgctc atgtattgct ggatcttctc ccgagcttct tctgattgcg attccctgta
     4921 ttttctttta aggtggggaa ttatcccttc aaaaacgctc ctgtgttccc acaggccgtc
     4981 ccggtcccgg ggcacatact taaaaagtat agcttcaata ctcccattca aaattatctg
     5041 cacatgctca ggtttaagtt ccgaaaaagg cgcatcaagt gaaaaatcat aatgtttcgc
     5101 aacggactgg agcatctggc ggtaataccc atgctcgtat ccccagggtt ctatggcccc
     5161 ttcactgatg gatttatttt tatccggaat tatgaggtca ggatcaaatt ccatcgtgtt
     5221 cccaaggccc tggcaggaag gacacgcacc gcgcggacta ttgaatgaga acatgtttgg
     5281 ttctaagggc tcaaaactga ttccgcattt tacgcatgct gcatgctcgc tgaaaagaaa
     5341 ctctttgcct tcaagcgcct gcgccactgc gatgccattc ccttcacgaa gcgctgttgc
     5401 cacagaatct gcaagccttt cttctatacc tttttttatt atcaggcggt caatgataac
     5461 atcgatatcg tgtttttgct gtttgttaag cgtgatctcc cgggtcagtt catggatatt
     5521 tccgtctatt cgaaccctgc tgtatccgga ttgtgaaaac ccctcaaata tctgtttata
     5581 ttctcctttc ttctgcctgg cattaggggc aagaatatga agctttgtac tttcaggaag
     5641 cgacatcaac cggcttacta tttgttcaac ggtctgcggt gttatctcac ttttacaatt
     5701 cgggcagtgc tgttttccga ttcttgcata aagaagacgc aggtaatcgt aaatttccgt
     5761 gactgtacct accgttgaac ggggattctt gcttgttgat ttctgttcga tcgagatggc
     5821 gggggaaaga ccttcgatat attccacatc tggtttatcc atttgtccaa ggaactggcg
     5881 ggcatatgct gatagcgatt caacatacct tcgctggcct tcggcataaa gtgtatcaaa
     5941 agcgagggaa gatttccccg aacccgaaag tccggttatg acaataagct tgttcctggg
     6001 cagttccagg tcgatgtttt ttaggttgtg tacgcgtgcg ccttttatta tgattttatc
     6061 tatggacata atggaatggt atcatattgc atcaaaaggc taaagaatta ttggcaatga
     6121 aaaaaattaa atattattga gttgaaaata gccatggggt aaataatatt agaaatacaa
     6181 tattgatcat tgcagttata ctgttaatgc tccccataat tcttttatgg gtaactggta
     6241 actctgcatt agataaattc ggcataacga aaatctatga gactgttttt ggcggcagag
     6301 aatggacatc gcaatgggat aacggtcatg cccggacatg gactgactca tcaaacgatc
     6361 cctatgacgc tgagttcgtg acacaagaca aaggaactgg ttcctataag gccgatggac
     6421 ttggtacatt aaaaatttct ggtgatggcc caaggatgta tgtcttagat tctaataaaa
     6481 tcaaaaactg gcacaatgtt gagattaccc tctatggtcg acgtgtttca gatgacaaca
     6541 ctacgtgggg gggaatcatg gcatatgcaa gaacgaatca cttgattgat tcagattatt
     6601 gtgataccag aggatatgga ggaagattcc gctatgatgg ggatattgat tttgaaaagg
     6661 aaacaagtca cccagactca gtggcggtaa gcaataaagt aaatccaggt ggattcccga
     6721 aaaatgtctg gatcgggtat aagtatgtcg tgtacgatat gtctgatggt aatatcaaac
     6781 tagagttata tagagacaac acagatggag tgaatggtgg aacctgggtt aaaataaatg
     6841 aatttattga cactggatca aatttcggag ttgcaggtac gtcgtgcaag tctggtatta
     6901 atccttttct tagattaact gcttctgata ctcgtccagg atcagaaaca ggaaaaccca
     6961 accttgatat ttatttcaga agtgatggag taggtagcga tggcctctgg tacaagaaag
     7021 caagcattcg ggagatcgta ccaatccgaa cagggtcttg acaattatac ctgtaccctg
     7081 tgaacgttcc tatacgacaa caattaaaaa aaaattcaat gccataaaaa aatttacatg
     7141 atgataaaaa tatttaaata tacctaacaa caaagattag tagttgtccg gataggaagt
     7201 attgttattc agaaaaggtg tgtgtggcaa taatataaac atttcctatg aaaaaggcaa
     7261 attaaaaact tattgttaaa acgtgtccac ggaaatgttc tgtgtactag aagaaaaatg
     7321 ccatctaata gaggttttga aatatagaaa ttcaaccaat ttaatctata ttaaatatat
     7381 gggggagaaa tcaatgaata tcaatatctt aggcagcggg gtagtaggcg ctgctacagg
     7441 aaagggattg aaaaatatag gaaataataa tgtgttgttc tatgatatcg acacaaatag
     7501 aatagaaagt ttgaaagaac aagcacttaa tgctacgact gatctggacc acgcagtgaa
     7561 aagttctgaa attttcatta tatgtgttcc cacgcccgat aagaataaaa tgatagattt
     7621 gagcataatc gaatctgcgg ttaaggaact ttcaaaagag tgtaaggaca aaaaagatta
     7681 tttccttatt gttataaaga gtactgtaat tccaatgact actgaaaatt taatcatacc
     7741 tttgatagaa aaatattcca ataaaaaagt tggaaatgat tttggagttt gctttaaccc
     7801 agaattttta agagaggcaa atccctatga agatttcatg aatccagata gaatagtaat
     7861 tggagaatat gacaagaaat caggagatat tcttgaacaa gtgtattcac ctttcaaatg
     7921 tccaatcata aggacaaatt taagaacagc agaatttgca aaatatgcaa ataattgttt
     7981 ctatgcgaca aagatcagtt tctttaatga aatacactta atgtgcaaac atattggcat
     8041 agattccaat attataagaa aggtggttca gatggacaaa tattatggaa tccatccatg
     8101 ggaacacgga catagtttcg gtggaaagtg ccttcccaag gacctgaacg ccgtcatagg
     8161 acttttcaat gaaggccata ttcatgatcc aatcctgtta aaggccgtta gaaaaatcaa
     8221 tgagataata gaataagaga tatttatttt gcattttaat atacaattat aggtggtcta
     8281 attatatgag acgaggatat cgaaaatttc ttagatataa tgttctggca ttttttttca
     8341 taggagtcct tcttgcaata ttatttatca aaaatttgac cctcttaaaa caagatactt
     8401 tatcattttt atattctata ttcgttacaa cgtttgtatt gtcaagaata acgggatctt
     8461 ttttttataa ttatgataat aaatggaaag acaataacta tgaacctgca gttagcttcg
     8521 taatacccgt aaaaaacgag gaaaatgtca tatcgaatac aatagctaaa tgttatgaag
     8581 tcgactatcc taaagataaa attgaagtta tagtaatcaa tgacggtagc accgataata
     8641 cattaaaaga aattgaaaat gcaaaaaaaa tctataaaga tttaactatc atagactgga
     8701 aaataaataa aggtaagaga tatgggatgg ccgaaggttt taaaatagca aagggagaaa
     8761 ttgtaattca gttagacagt gacagttata tagagaagtc ctctctaaag acattgatcc
     8821 gaccgttcca ggatggagat attggagcgg tagctgccca ttcggaccct cagaatgcaa
     8881 atgaaaattt tcttaccaaa atgcaggcag catactattt tatatccttt agagcaatga
     8941 aagcttctga gagtttattt gatacggttt tttgttgtag tggatgttgc tcagcatata
     9001 gaaaaaatat tgtattgccc gtattagata aatggataaa tgagactttt ttatctaagc
     9061 ctgtttcatt tggtgacgat agatctttga caaatcagct attgaaagcc gggcataaaa
     9121 ctatctatct caataaagcg caggcatata ctatagtacc caataatatt agacaattta
     9181 tcaaacaaca aattcgatgg aaaaagtcct gggtcattaa tgcaattttc ctctcacaat
     9241 ttatatttaa gagaaataaa tttgttgcca taacatattt tattcctgtt attataattt
     9301 cattgtttac cccctttata gcgtttaagg caataatact aaacccggtg atatttggta
     9361 tatctccgat tttctatatt attggggttt ttctagtgtc aagtctattg gcaatacatt
     9421 atatgttttt tagaggtgat aaaaattata aatatatata tgcatggata tttttgggtc
     9481 tggtatttac atcttatctt atgttctatg cgttgttaga tataagaaat atgaagtggg
     9541 gaacgagatg atatgaatga aaataaaaga taataaattt ttgaataata atcgtataaa
     9601 gctatatttt tcacttatga ttttggtttg gataattttg ataatatctt caccgtttat
     9661 tatggaaaga gtcgtttcgg ctattaatcc taacacatat tcgcgtattc cgttaattgg
     9721 ctattatcaa caatctaatg tgaattcatt aaatacacta atagatataa atgataattt
     9781 atcttgtcaa ccaaaatttc caatagagaa aaccatcatc ctaagaatgg acgatgtcca
     9841 gaaatctgct tatgatgatt tagtaataaa ccttactgaa actgtcttga gtaaaaatat
     9901 gtccattact cttggcgtaa tacctaaaaa tattaatgat gattctatta tcaaaacata
     9961 tctcattggt aaaatgaatg atcccaggat agaaattgcg caacatggaa caaatcatac
    10021 atttctagaa tttcttccaa acataacaaa acaggaagta tatgatatga caaaacccgg
    10081 tctagatcaa ataataatgg cattgaatgt atatccggtg acatttatac ctccaaataa
    10141 tgcttataat gaaaaggctc caggagcaat caaggaactt ggttttaaaa tactttctgc
    10201 caaaaaaggc gagtttaaat ttagtgatgg tattttgtat gcaggatttg acattgcaac
    10261 gaaatactca agc
//
LOCUS       DATGBN010000474         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_503449, whole genome shotgun sequence.
ACCESSION   DATGBN010000474 DATGBN010000000
VERSION     DATGBN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503449"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..446
                     /locus_tag="VKL21_12085"
     CDS             201..446
                     /locus_tag="VKL21_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46548.1"
                     /translation="MFPPHNVKLLIFLVNNNIKPVRGIHELYFTQDILFDYTIDSYKA
                     PALNTKSRCFEILTAIEDIKEERLLSGTLKPMLEEWL"
     gene            complement(503..703)
                     /locus_tag="VKL21_12090"
     CDS             complement(503..703)
                     /locus_tag="VKL21_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46549.1"
                     /translation="MGYSYASCHQRQSILEVVAALRIGTAKTEVLTHSGFKIYSNGRF
                     HARLETDTGDWLDTTVKKHCPW"
     gene            853..1272
                     /locus_tag="VKL21_12095"
     CDS             853..1272
                     /locus_tag="VKL21_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46550.1"
                     /translation="MPYFDITNKRPRINIFKISGAYYFKYFFDDPELFRELEPFYDKS
                     RYRFKMATAGERNKVMKLLDMKGYDPNIIDDPAHYTVEIYKYQKYGELLKNSVDNYPL
                     REKIVFVMKDMACVEQVLAMGAVVSASLKNSHLFEKH"
     gene            complement(1462..1791)
                     /locus_tag="VKL21_12100"
     CDS             complement(1462..1791)
                     /locus_tag="VKL21_12100"
                     /inference="COORDINATES: protein motif:HMM:NF020645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46551.1"
                     /translation="MPPLYSQKKKNERVKLCDVFFEYQRLTTELNFTCMSPNQVADKI
                     REFDMLGLLKNKYVRKGSGQIKYIDINPNHQSQFLIHISVLQEEIKNIKPSELATEEL
                     IKKYLNA"
     gene            complement(1940..2326)
                     /locus_tag="VKL21_12105"
     CDS             complement(1940..2326)
                     /locus_tag="VKL21_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator protein"
                     /protein_id="HMB46552.1"
                     /translation="MKTIQIKQFDEKDERIAEALISLGMNKNVAMALTFLQNMNAATS
                     TDIERGVNLRQPEVSIAVKQLKEKNWITERGERKIGKGRPIKVFSLKVGFNDIVNQLE
                     KQHEKALDEARVNVERLKELENNSKN"
     gene            complement(2372..2512)
                     /locus_tag="VKL21_12110"
     CDS             complement(2372..2512)
                     /locus_tag="VKL21_12110"
                     /inference="COORDINATES: protein motif:HMM:NF036315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5371 family protein"
                     /protein_id="HMB46553.1"
                     /translation="MHVQSVLPQEDVIALKKKTGKSSVKEAISKAVYYYLEESVSNTN
                     DN"
BASE COUNT          827 a          530 c          453 g          925 t
ORIGIN      
        1 tgcttgcgag atatatgcca gaagtccttg acatagaaaa aggtagtgtt gcacccataa
       61 aaactgaaga ctttaagcaa caattaatgt atagaattaa ttcaatggct aaataattcc
      121 attatatttt ttattccgcc ttcaaacgca tattccagct tcttcgtaag ttggtttatt
      181 actttttgct gtttttcttt atgttcccac ctcacaatgt aaaacttctc atcttcctgg
      241 ttaataataa cataaagcct gtaaggggga ttcatgaact ttatttcacc caggatatac
      301 ttttcgatta tacaattgac agctataaag cacctgcttt aaatacaaaa tcacgttgtt
      361 ttgaaattct aacagcaatt gaagacatta aagaggaacg cttactttct ggaactctca
      421 aaccaatgct ggaagaatgg ctctgaaaat atatactgtt gagaaatatc ctgtagatta
      481 tggcctgtta ctacgcaagc tgtcaccaag ggcagtgttt tttgactgtt gtgtccaacc
      541 agtcgcctgt atctgtttcc agtcttgcat gaaagcgacc atttgagtat atcttgaatc
      601 ctgagtgtgt taatacttcg gttttcgcag ttcctatacg caaagctgcg acaacttcca
      661 aaatactctg cctctggtga cagcttgcgt agctataccc catcaacggg atttctccat
      721 gttggcaaaa aggatgacga tatttcaaat ctggtgtgag ttccagtcga catcacacac
      781 acatgcgatg tccattggaa cttttgtatc ataatcatag cgtttatccg ctaatagacc
      841 caagaaatac taatgcccta tttcgatata acaaacaagc gcccccgcat caatattttc
      901 aagattagtg gagcgtatta tttcaaatac ttctttgatg atcctgaact attcagggag
      961 cttgagccat tctatgataa atcccggtac aggttcaaga tggcaacagc aggtgaaaga
     1021 aataaggtaa tgaagctcct tgacatgaaa ggctatgatc ctaatataat tgatgacccg
     1081 gcgcattata ctgtggaaat ttacaagtac cagaaatatg gcgaacttct caagaattca
     1141 gtagataatt atcccctgcg ggaaaagata gtgtttgtca tgaaggatat ggcatgcgtt
     1201 gaacaagtcc tggcaatggg agcggttgta agtgcctcgt tgaaaaattc acacttgttt
     1261 gaaaagcact gaggaagatt tatatatata aactcattat atttattttg acttatccag
     1321 gtcaggaggc aataaaccgt atggcgaaga aagagaaacc gaaagaaaag ccaaagccca
     1381 agaagtaaca cctaattaaa tatctttttt tttttgagta aaagattacc gatcaactaa
     1441 tttaccactt atcgaataag tctatgcgtt cagatatttc ttgatcaact cttcggttgc
     1501 aagctccgaa ggtttgatat tttttatttc ttcctgaagc acagaaatgt gtatcaaaaa
     1561 ctgagactga tgattgggat taatatcaat atacttgatc tgaccggaac ctttacgaac
     1621 atacttattt ttcaaaagtc caagcatatc aaactctctg attttatcag caacctggtt
     1681 tggtgacata caggtaaaat tcaattcagt cgttaatctc tgatattcaa agaaaacatc
     1741 acataacttg accctctcat tcttcttttt ctggctatat aacggaggca cgcatacaat
     1801 atcaatttga agtgaatcgg cagtgacctg attacttcaa gtacaacttc agaatgtgat
     1861 gtgggtgggt tgtagatctt tctttctgtc tcttcttctg ttccattttt cagaaataaa
     1921 tattccataa cactgaagcc taattcttgg aattattttc caattctttt aatcgttcaa
     1981 catttaccct ggcctcatcg agagctttct catgctgctt ttcaagctga ttaacaatat
     2041 cattaaaccc gactttaagt gaaaatactt ttatgggtct tcccttccca attttccttt
     2101 ctccccgctc agttatccag ttcttttcct ttaattgttt cacggcaata ctgacttcag
     2161 gctggcgtag attcactcct ctttcgatat ctgttgaggt agctgcattc atattttgaa
     2221 gaaaggtcag agccatagcg acattcttgt tcattcccaa ggaaataagt gcttctgcaa
     2281 ttctctcgtc tttttcgtca aactgtttaa tctggatggt tttcatgatg actcctatta
     2341 ttattcatta ttaatattat atttaatctt ttcagttatc gtttgtatta gacacacttt
     2401 cctctagata atagtaaact gcctttgaaa tggcttcttt gaccgatgac tttccggtct
     2461 tttttttcaa agcgattaca tcttcctggg ggagaactga ttgaacatgc atatatttca
     2521 taagattata tcctaagata aaaataattc tatctataat tattaataaa ttgttaatat
     2581 acaattgctg agttaaccta taaataattt ctatatataa ataattatta tcatttatat
     2641 catgagtatg tattaaaatt aatacagtca tactacattt ttatggtaag attgtagtat
     2701 ttattctcaa tggcccccct ctctccttcc cccgg
//
LOCUS       DATGBN010000475        12338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_503520, whole genome shotgun sequence.
ACCESSION   DATGBN010000475 DATGBN010000000
VERSION     DATGBN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12338
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503520"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VKL21_12115"
     CDS             complement(<1..221)
                     /locus_tag="VKL21_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46554.1"
                     /translation="MIVRLMGEGQYELEKKHVDEVNKIDNNIVKIVNTGNEKDFKSEF
                     KKLSEYVRKNGKKISDDIIKPSGIIIPPS"
     gene            complement(218..919)
                     /locus_tag="VKL21_12120"
     CDS             complement(218..919)
                     /locus_tag="VKL21_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HMB46555.1"
                     /translation="MGLIDRTSTIIQAKINKILGKFEDPRETLDYSYEKQVELLQNVK
                     RGVAEVTTSKKRLELQKIKLEENIKKLDDQARESIKAGREDLAKIALERKGASQAEIG
                     SLTLQIADIKNQQDKLVATERRLSAKIESFRSTKETIKAQYSAAEAQVKITESVSGIS
                     EEMSDVGMAVQRAKDKTENMKARASALDELVESGTLEDVTGGSQDDIDRELSKIKSKG
                     DVDIQLEALKKEMGK"
     gene            1280..1942
                     /gene="aroD"
                     /locus_tag="VKL21_12125"
     CDS             1280..1942
                     /gene="aroD"
                     /locus_tag="VKL21_12125"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HMB46556.1"
                     /translation="MEFPCNRSVVASIGKNPVNTSIKAKDLGADILELRIDLIREDPI
                     RILKDLKELSLPVIITNRMKKEGGAWTGSETRRIQELVSLIPHADAVDIELGAPNRDL
                     VVMKAKNAGKKIIISTHDFQSTPDLKTMADSIRGSFDAGADIAKLAVMPHSLDDVLHL
                     LEVTLHSSGPVCTIAMGEIGKHSRAISPLYGSVMTYGYVDIPVAPGQLRVDELKYMLK
                     LL"
     gene            complement(1948..2862)
                     /locus_tag="VKL21_12130"
     CDS             complement(1948..2862)
                     /locus_tag="VKL21_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMB46557.1"
                     /translation="MNWKILQLAILFTIWISIANAAPDNYKVIYTINVKENGSAIWNV
                     EYRTLLSTQDEQYSFENYTSQLNSKYLVEFKELMERSAQEASNGTSRKMDATDFKGEA
                     NIQSTPTGKYGVVHYFFTWNNFAKIDQKINIGDVFVGGLYLSNDNTLIIQYPPGYTLE
                     QVIPQPDKSGDELVWYGLRSFGKGEPWIILNKPSFPWITVVMGIIIVLSMAAGMFLIK
                     RKKEGEGNIPQDIPETNIEILETEMMDLEDRILKLLKENGGALYQSELGRQLDLPKST
                     VSSALNQLHDKNLIRKIKKGRENLIRMI"
     gene            complement(2999..4150)
                     /locus_tag="VKL21_12135"
     CDS             complement(2999..4150)
                     /locus_tag="VKL21_12135"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HMB46558.1"
                     /translation="MNTMRFTVTGIILLAFIMNIIGAAQEDKSPDELVDDIEEYNGSI
                     GPENSLYGLKLAFEGLDESFTLNTTEKLEKKIYHSRLRISEAKTELNKKNDDGAKKAF
                     EYYDEKIKETENSISEQKDNNSGMLNAQKMITKHQYILERLLELHPNNSGLMNAYNNS
                     VELENTFEKKTDRKLERILTKEGKPYLKEVTKEDTENSEDNDLQYFNYELKLTASPKN
                     NNTQVKVELKFRSNKTDNYSIADEIINEIGLSKENISSVIKIDDLNNNETKSMIEAQA
                     SVGSNISNIMVDYSFLLNETNRTEVIDSIHNKLSNLTKKQILGVLEIKTLQEEIKSEE
                     NDNRLNENDSELQENGSDKMPEQLSGKKDKMEDDNFGTTRQEKDNKRDD"
     gene            4431..5948
                     /locus_tag="VKL21_12140"
     CDS             4431..5948
                     /locus_tag="VKL21_12140"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HMB46559.1"
                     /translation="MELKVQSLQIKAGKYKVVINGQDAIELGVRGGDRVQLKDHSYLT
                     AIVETGDMIPKGTVGVYQECCEKLGKVCPLTIDLIPTSRPNSVGFIKKMMDHQKLTQE
                     EIHMIVQDIVRENLTEIEMSAFVTSSYIHGMTSDEVEWLTRAMISTGEKIEFDTHPIM
                     DKHSIGGVPGNKISLLVVPIIAANGLLIPKTSSRAITGAAGTADLMEVLAPVEFTADE
                     IKTMTEKVGGVIVWGGATNIAPADDKLIRAEYPLSVDPHCQLLASIMAKKGAVGADCV
                     VIDIPVGSGTKVHSMEEGRKMARDLIDLGERLGMKVECAMTYGASPVGRTIGPAIEVR
                     EALKVLETMQGPNSLIEKSTGLAGILLEMGGVATRGRGKEIALETLRSGKAFEKLKQI
                     IEIQGGNPNIVHTDIKPGDQRGELTSPADGYVIEFDNKRIVELARIAGAPADNGAGVW
                     IHRKKGEMVKKGEPLITIFADKSWKLTNALKTAEKDYPIIVEGMLLERIQTFKVF"
     gene            6068..8002
                     /locus_tag="VKL21_12145"
     CDS             6068..8002
                     /locus_tag="VKL21_12145"
                     /inference="COORDINATES: protein motif:HMM:NF010366.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HMB46560.1"
                     /translation="MNIKQNKQKSNATFELEESHLFWCRKCNVPLLEEMCAICREKGT
                     KVDLSRPGDVRFASPHETKLMDDLILRSFGENPIVEKLILLNKIPGEDKTDEIIVDGL
                     HFGILRFDMSELAFKLDLMLEGACALIDSGINKKIIRISSCGRHLSGKNIDGNEVIEC
                     SDDIGKGDTILVTDKNLCGFGVAYRDSCELKDPGLSLKIRKIGSGKVTFLPGKPLLGD
                     VIRANAPHMKQIVKDAVNTIRGIANQMEFRETPVYVSFSGGKDSLVTLDLTRSAVKKP
                     IKVFFANTGIEFPETVDFARRFCKENNIELIEVKVNEAFWENLPSFGPPAKDFRWCCK
                     VCKLAPINSVIEDCTRTGKKCLTIDGKRRFESFERSRIAPKEENPFIPGQVSLFPIRN
                     WRAIEVWLYIFYRKIEYNPLYDEGFERVGCWLCPAELSAEYYRFGQLHPELFTRWNEY
                     LLKWGKEHGLDEAFIKHGFWRWKTLPPKMLRLAEELKIKTKPQTPEEEFGITLTGGIS
                     PCRTGGYTMEGKVTGLLPQEVQNIANILGENTFSEDLGVLLIHAKGGNTRIFSSGHIS
                     VNAPGKENARSVFENTAKQIIRVKKCTKCKVCLKVCTVNAIVLEPDLKIGKVCTRCGK
                     CTESCVVVKYFDRILPDFKH"
     gene            8315..8557
                     /locus_tag="VKL21_12150"
     CDS             8315..8557
                     /locus_tag="VKL21_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46561.1"
                     /translation="MNARKTKLPDNSRGLQNIQHIVGGTYNILLVLVGVSFGKNDTSL
                     GIVMAVLTFIVARLSSEISFQTSIKVVREYSNSKEN"
     gene            complement(8566..8949)
                     /locus_tag="VKL21_12155"
     CDS             complement(8566..8949)
                     /locus_tag="VKL21_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5402 family protein"
                     /protein_id="HMB46562.1"
                     /translation="MITEELLKNRAGFEDKMRKLIGRPVLLIELDVFALPCGCAGITA
                     NMRGLEIDDLDVFEPAILPLVKEMAANLGIKPTVIFARVVPGSSIVASLNWRMLCPRC
                     YPEFARGEGKMPRPDLYMLQFERRK"
     gene            complement(8946..9980)
                     /locus_tag="VKL21_12160"
     CDS             complement(8946..9980)
                     /locus_tag="VKL21_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HMB46563.1"
                     /translation="MKLFVPSPGHIDSLKELLKQKEKIYSIFMAGSPDYIGTGRSNLA
                     SPQLEDIKEQTEFAHKNGVKMELVLNSSCMGGRQLTPEGFRINHWYIEKLVDIGIDSI
                     VVADPYLVETISRDFDVDVVVSVLAFVDSPQKAEFFEDLGAKSIVIDSNVNRHFDVLH
                     AIRDSVDCELKLLVNEGCLYRCPFRYAHFNFFSHAFGPEPRPNVLDDYYYLKCLELRI
                     KNPELITKSPWIRPEDLKLYRDITDVYKIGGRTHFVDWILNCVNAYYNESYDGNLMDL
                     LDCPKDLKDLYNIPNKSFDGILEKQWKNCRKVCNKCGYCRTLTKKVVQVYSNGGTGFE
                     KCLPWVEVKT"
     gene            complement(10173..10517)
                     /locus_tag="VKL21_12165"
     CDS             complement(10173..10517)
                     /locus_tag="VKL21_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HMB46564.1"
                     /translation="MLEIKSSFVTIELPSFHFDVCSLFAFLRYKYLSNRKTTDTNMAK
                     VKMLTAVLHREEDMYVAECPEVGTFIQGKTIEEAVDNLKEATELYLGEFPMETQAKSI
                     ITTFEVSPVVEA"
     gene            complement(10519..10755)
                     /locus_tag="VKL21_12170"
     CDS             complement(10519..10755)
                     /locus_tag="VKL21_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HMB46565.1"
                     /translation="MSEIIPDDTLDVRGECCPYPLILTKKRVEQLKSGEILLITADDP
                     VAPQNIDSWAKKSGNKILAVKQDGKIFNVYVQRI"
     gene            10891..11367
                     /locus_tag="VKL21_12175"
     CDS             10891..11367
                     /locus_tag="VKL21_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 domain-containing protein"
                     /protein_id="HMB46566.1"
                     /translation="MKQIETASGIGGMLTAFRKLARGRKKITFIGCPGWCNPFAELLG
                     YVLRDAGKEMVFIPNLRKDLAANIQMTVYGMQIGEKADPSADTVVLLGGLAMPKMEVD
                     VRGLNKMIEEITRGHPDPQILGVCIGGVFQKAGWDKLIDFDYLMDADMEVVTYEFK"
     gene            11389..11805
                     /locus_tag="VKL21_12180"
     CDS             11389..11805
                     /locus_tag="VKL21_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMB46567.1"
                     /translation="MNKLARLFIKTSLVYLVLSAILGVLITIGPGYSFMHSHFAVVGW
                     VSFMLFGLAYESIPRYTSRAIFSKKLGMIQFWLANIGLIGLSVSYSFMRMYMLKEKDY
                     SDAITALMIFGLIEAISCFIFVYNIWKSMGNTGTSK"
     gene            11852..>12338
                     /locus_tag="VKL21_12185"
     CDS             11852..>12338
                     /locus_tag="VKL21_12185"
                     /inference="COORDINATES: protein motif:HMM:NF020400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HMB46568.1"
                     /translation="MEWKADAIKELEKVPENVRNMAKMGIETAVEKKGENIVTLKDVN
                     EAAAQFRAIMEKEEKTTKIAVVRCDIVSETCPGVACFKAFNKRKVHFEQYGKDAQIIG
                     FFTCGGCPGRRIFRLVDSLLKHDVDVVHLSSCMLLEKSYAKCPHIEEIKQMITVKGVK
                     IV"
BASE COUNT         3566 a         2543 c         2532 g         3697 t
ORIGIN      
        1 tcagagggcg gaatgatgat cccgcttggc ttgattatgt catccgaaat tttttttcca
       61 ttctttcgga catactctga aagtttcttg aattcgcttt tgaaatcttt ttcatttcct
      121 gtatttacta ttttcacaat attattatcg atcttgttga cttcatccac gtgctttttt
      181 tcaagttcgt attggccttc tcccatcaat ctcactatca ttttcccatc tccttcttta
      241 acgcttcaag ctgaatgtca acatctccct ttgattttat ctttgaaagc tcacgatcaa
      301 tatcatcctg tgaccctcca gtcacatctt caagcgtccc tgactcgaca agttcatcaa
      361 gagcagaggc ccgtgccttc atattctcgg tcttgtcttt tgcccgctgg accgccattc
      421 ctacatcgct catttcctcg ctgataccgc tcacggattc ggttatcttg acctgcgctt
      481 cggcagcgct gtactgggct tttattgttt cctttgttga gcggaaggat tctatctttg
      541 ctgacagacg tctttctgtt gcaaccaact tatcctgctg gttcttgata tccgctatct
      601 gcaatgtgag acttccaatt tctgcctggg atgctccctt tctctcaagc gctatttttg
      661 caagatcttc ccgccctgct tttatggatt cacgtgcctg gtcatccagt ttcttgatat
      721 tctcttccag ttttatcttc tgtagctcaa gcctcttctt cgaagtcgtg acctctgcaa
      781 cgccgcgctt cacgttctgt aaaagttcaa cctgcttttc ataagaataa tcaagagtct
      841 ctcttggatc ttcaaatttt cctaggatct tattgatctt tgcctgtatt atcgtacttg
      901 ttctatctat tagccccata ttgttacctc tcctttcata ctatttctca aacctaaata
      961 gtttgttcct taacaaaatc gtatccacag ggttcatgcg ctttttccca ttcagtcctt
     1021 ggatatttcc tgcactgtag aggccttaaa ccatggatgg tgcatataga tttatgtgaa
     1081 ccgtgatttt caaatttaag aaaaggacat ttaaatacga acatacagca agctccacat
     1141 cttttgcatt cccctacacg attttcagct acgggaagaa caaggcttga agctgctcta
     1201 ataatttttc cggataaact cttcatcatt ttcttttatt tcataatcct tatcacatat
     1261 taaagatatc atatctgcta tggagttccc ctgtaataga tcagttgtgg catcaattgg
     1321 gaaaaacccg gtaaatacat ctatcaaagc aaaagacctg ggcgctgata tacttgagct
     1381 gcggatagac ctgattcgcg aagatcccat tagaatactt aaggatctta aggaactcag
     1441 cctgccagtt attattacca atcgaatgaa aaaagaaggc ggcgcatgga caggaagcga
     1501 aacaaggcgc atccaggagt tggtttcctt aataccgcat gcagatgcgg ttgatattga
     1561 actgggcgcc ccaaacagag atctggtagt tatgaaagca aagaatgccg gaaaaaaaat
     1621 cataatttcc acgcacgatt ttcaaagcac gcctgatctt aagacaatgg cggattctat
     1681 caggggatca ttcgatgcag gtgctgatat tgcaaagctt gctgtaatgc cccactcgct
     1741 tgacgatgtg ctgcatttgc ttgaggtcac attacattca agcgggcctg tatgtacaat
     1801 cgcaatgggt gaaattggaa aacactcacg tgccatttct cccctatacg gttctgtcat
     1861 gacctacgga tatgtagata ttcctgtagc tcctggacaa ctaagagtgg acgaactcaa
     1921 atacatgctg aagcttctct gatctggtca gatcatacgt ataaggtttt cccgcccttt
     1981 cttgatcttc cggatcaggt ttttatcatg tagttgattc aatgccgagc tgaccgttga
     2041 tttcggaaga tcgagttgtc ttcctaattc ggactggtaa agggcgcccc cattttcttt
     2101 caggagtttc aggatcctgt cttccagatc catcatttca gtttccagaa tctcgatatt
     2161 ggtttcaggg atatcttgag ggatgtttcc ttctccttct tttttgcgct ttatcaagaa
     2221 catcccggca gccattgaaa gtacaattat gatacccata acaactgtta tccatggaaa
     2281 tgatggttta ttaagtatta tccagggttc tcctttcccg aatgatcgaa gaccgtacca
     2341 gaccagttca tccccgctct tgtcaggttg aggaatgacc tgttccaggg tataacccgg
     2401 aggatattga attatcagtg tattatcatt tgaaagatat aatcctccca cgaatacatc
     2461 tccgatgttg atcttttgat cgatcttagc aaagttgttc catgtgaaaa agtaatgaac
     2521 cacaccatat ttgcctgtag gagttgattg tatattcgcc tcccccttga aatccgttgc
     2581 atccattttc cttgatgtcc cattggatgc ttcctgggct gatctttcca tcaattcctt
     2641 aaactcaaca agatactttg agttcaattg cgaagtataa ttctcaaagg aatactgttc
     2701 atcctgggtg gaaaggagtg tacgatattc cacattccat atagctgaac cgttctcctt
     2761 aacatttatt gtatatatga ctttataatt atcaggcgca gcatttgcta ttgaaatcca
     2821 gatcgtaaaa aggatagcaa gctgcaggat cttccaattc ataagattca cttggttgaa
     2881 tagatagtta ttgttcttca agcaataata atctatttac gattaatctg tccataaaaa
     2941 gatgggtaaa aatccatctt ttcttttctc cttctgatca cgcatcagag agttcccttc
     3001 agtcatctct cttattgtct ttttcttgtc ttgttgttcc aaaattatca tcctccattt
     3061 tatctttttt accggacaac tgttcaggca ttttatcgct accattttcc tgtaattcgc
     3121 tatcgttttc atttaatctg ttatcatttt cttcagattt tatctcctct tgaagcgttt
     3181 ttatttccag aaccccaaga atttgttttt tggttagatt tgagagctta ttgtggatac
     3241 tatcgatcac ttctgtccgg tttgtttcat tgaggaggaa actgtaatca accatgatat
     3301 tggatatatt tgaaccgaca ctggcctgcg cttcaatcat cgattttgtc tcattattat
     3361 tcaaatcatc gatcttgata acacttgaga tattttcttt gctcaaccct atctcgttta
     3421 tgatctcatc tgctattgaa taattatccg ttttattaga cctgaatttc aactccacct
     3481 ttacctgagt attgttattt ttcggacttg cagtcaattt caattcataa ttaaagtatt
     3541 gaaggtcgtt atcttcacta ttctctgtat cctctttcgt tacttccttc aaatagggtt
     3601 ttccttcttt tgtcaggatc cgttcaagtt ttctatccgt ttttttctca aacgtatttt
     3661 caagctcgac gctgttatta tatgcattca ttaatccgga attattcgga tgtaattcaa
     3721 gaagtctttc caggatatac tgatgttttg tgatcatttt ctgggcgttg agcattcctg
     3781 aattgttatc cttttgttct gaaattgaat tttcagtttc ctttattttc tcgtcataat
     3841 attcaaatgc cttctttgcg ccgtcatcgt ttttcttatt caattcggtc tttgcttcag
     3901 atatccgaag tcttgaatgg tatatttttt tctcaagctt ttcagtagta tttaaggtaa
     3961 aactttcatc cagtccctca aacgcaagtt tcaacccata caatgaattt tcagggccaa
     4021 ttgagccatt atattcctct atatcatcta caagctcatc aggtgattta tcttcctgtg
     4081 ccgctccaat tatattcatg atgaatgcaa gcaggattat tcctgtcacc gtaaacctca
     4141 tagtattcat ttcttatacc tcaaagcatc tatctggtgc caaatttaga taatcctgtt
     4201 agaatataaa catttttaca gtataaatag aattttaaag tacacattaa ttcataagag
     4261 tcctaaaagg ttcaataaag ctttgttttg ttcaatatcc aataataata ctgcgattca
     4321 ttcaagatgc actggtatat tttaatattt catcatattt tcagcgatag tgctaagtat
     4381 tgcaagtact aaattgatta aataaaataa aattacgagg gtactataaa atggaactta
     4441 aagtgcagtc acttcagatc aaagcaggta agtataaagt agtaatcaat ggccaggatg
     4501 caatagaact tggagttcgg ggaggggacc gcgtccagtt aaaagatcac agttatttga
     4561 cagccatcgt tgaaacgggc gacatgatcc caaaaggcac tgtgggagta tatcaggaat
     4621 gctgcgaaaa actcggaaaa gtgtgtcctc ttacgatcga tcttatcccc acttcaagac
     4681 cgaattcagt gggtttcata aagaaaatga tggatcacca gaagttaacg caggaagaga
     4741 tccacatgat agtgcaggat attgtaagag agaatcttac ggaaatcgag atgtcggcat
     4801 ttgtgacaag ctcatatata catgggatga cttcggatga ggtggaatgg ctgaccaggg
     4861 ctatgataag caccggggaa aaaattgaat tcgacactca ccccataatg gataaacaca
     4921 gcataggagg agttcccggc aataagatat ctctgcttgt ggtcccgatc atcgccgcaa
     4981 acggcctcct catccccaaa accagttcac gcgcaataac tggcgccgca ggaacggctg
     5041 accttatgga agtacttgct cctgttgaat tcacggcaga tgagataaag accatgacag
     5101 aaaaagtagg aggcgtcatt gtttggggag gagctacaaa tattgcgcca gcagatgata
     5161 agctaatacg ggcagaatat cccctttctg tagatccaca ctgtcagctt cttgcttcaa
     5221 taatggctaa aaaaggtgcc gttggggccg attgtgttgt tattgatatt cctgtgggaa
     5281 gcgggacaaa agtacattct atggaagagg gaagaaagat ggcgagggat ttgatcgacc
     5341 tgggagagcg tcttggtatg aaagtagaat gcgctatgac atacggcgcg tctccggtag
     5401 gaagaaccat cggtcctgct atcgaagtaa gagaagcgtt gaaagtgctg gaaacaatgc
     5461 agggtcctaa cagcctcatt gaaaagagca caggacttgc aggcattctt ctcgaaatgg
     5521 gaggtgtagc gacaagaggt cgcggcaagg agatagcttt ggaaacactg cgttccggga
     5581 aagcattcga aaaactcaaa cagatcattg aaatccaggg tggaaatccg aatatcgttc
     5641 atactgatat taaaccaggg gatcaaaggg gagaacttac atctccggct gatggctatg
     5701 ttattgagtt cgataacaag cgcattgtgg aactggcaag gatcgcagga gctccggctg
     5761 ataatggggc aggcgtctgg atacacagga aaaagggtga aatggtcaag aagggcgaac
     5821 cattgataac gatttttgcg gataaaagct ggaaattgac aaatgcatta aaaaccgcag
     5881 aaaaggatta tccgataata gtagaaggca tgcttcttga aagaatacag accttcaagg
     5941 tattttaaat acaagatacg gaaatgagag atattgtaat tatttcatcg aatccacagt
     6001 accatcagtc gtaatatatg aaaaagatgt atactttggg gataaataag attgcagata
     6061 tataagaatg aacattaaac aaaataaaca aaaatctaac gctaccttcg aacttgaaga
     6121 aagtcacctt ttctggtgca ggaaatgcaa tgttccctta cttgaggaaa tgtgcgcaat
     6181 ttgcagggaa aaaggaacaa aagtagacct gtcccggccc ggagatgttc gttttgcttc
     6241 accgcatgaa actaaattaa tggatgacct tatcttaaga tcttttggcg aaaatcccat
     6301 agttgaaaaa cttatccttt taaataaaat ccccggtgaa gataaaactg atgaaataat
     6361 cgtggacggg ctccatttcg ggatattgag attcgatatg tcagaacttg ctttcaaact
     6421 tgacctgatg ctggaagggg catgcgcgct tattgattca ggcataaata aaaaaatcat
     6481 tcggatctca tcatgcgggc ggcatcttag cgggaaaaac atagatggaa atgaagtaat
     6541 agaatgttct gatgatatcg gaaaagggga caccatactt gtaacggaca agaacctgtg
     6601 cggtttcggt gtagcatata gggacagctg tgagcttaag gatcccggac tatcattaaa
     6661 gataaggaaa atagggtccg gaaaagtaac tttcctgccg ggaaaaccgc tactcggaga
     6721 tgtcatccgg gctaatgcgc cccacatgaa acaaattgtg aaagatgctg tcaatactat
     6781 caggggaatt gccaaccaga tggagttccg ggaaaccccc gtctatgttt ctttttcagg
     6841 aggaaaggac agcctggtca cgcttgacct tactcgcagc gctgtgaaaa agcccataaa
     6901 agtattcttt gcaaatactg gtatcgagtt ccctgagacc gttgattttg cacgaaggtt
     6961 ctgcaaggaa aataatattg agcttattga agtaaaagta aatgaagcat tctgggagaa
     7021 tctcccaagt ttcggccctc ctgcaaagga tttcagatgg tgctgcaaag tatgcaagct
     7081 tgcgcccatc aattcggtca tagaagattg cactcgcaca ggaaaaaagt gcctcacgat
     7141 cgatgggaag aggagatttg aatcattcga acgttcacgg atcgcgccaa aagaagaaaa
     7201 tcctttcatc cctggtcagg tatcgttgtt ccctatccgc aactggcgcg ccatcgaagt
     7261 gtggctttat attttttaca ggaaaattga atataatccc ctgtatgacg aaggttttga
     7321 acgtgtagga tgctggttat gccctgctga actctccgcg gaatattaca ggttcggcca
     7381 gctgcatcct gagcttttta cgcgctggaa cgagtatctc ttgaaatggg gaaaagaaca
     7441 tggactggat gaagctttta tcaagcatgg attctggcgc tggaaaacac ttcctcccaa
     7501 aatgctgcga cttgcagagg aacttaagat taaaacaaaa ccacagactc ctgaagaaga
     7561 attcggtata acattaacgg gcgggatctc accatgcagg accggagggt acacaatgga
     7621 aggaaaggtc acggggctct tacctcaaga ggtacaaaat attgcaaata tcctgggcga
     7681 aaatactttt tcagaagatc ttggcgtgct gctgatccac gcaaaaggtg gaaataccag
     7741 gatattctcc tccgggcaca tttccgttaa tgcccctgga aaggaaaatg ccaggtctgt
     7801 ttttgaaaac accgcaaagc agataataag agttaaaaag tgtacaaaat gcaaggtttg
     7861 tttgaaagtc tgtaccgtaa atgccattgt tcttgagcct gatctgaaaa ttgggaaagt
     7921 ttgcacccga tgcgggaaat gcactgaatc atgcgtggtt gtgaaatact ttgacaggat
     7981 cctgccggat tttaagcatt aaaagcagtc agttccataa ttgtatctct catcataaac
     8041 ttttttgatt atattttttt ctataaggcg tgaataataa tgcttatatc catagacatc
     8101 attatggtaa ttataatgat aaaagatatg atcgaatatg agtgtttggt atgtggacac
     8161 catatcaaga gtgattcttt aaagagaatg atgtgttatg aatgtggaaa tggaatctat
     8221 gcacctgtta ctacgctcct cgagcaatag taatcctgat gctgatatca ccctctttgg
     8281 tcataaatat taaaggcgga gaaaagagat caaaatgaat gcaagaaaaa caaagctacc
     8341 tgataactca agaggacttc agaatatcca gcatatcgtt ggcggaacct ataacatcct
     8401 attggtcctt gtgggcgtat ctttcggaaa aaacgataca tcacttggta tcgtgatggc
     8461 cgttctcaca tttatcgtag caagattatc atctgaaata tcgtttcaaa catcaataaa
     8521 agtagtaagg gaatattcca attccaagga gaattaggag aaagactatt ttcgcctctc
     8581 aaactgcagc atatacagat ccggccttgg cattttcccc tcgcctcttg cgaattcagg
     8641 gtagcatcga ggacagagca tacgccagtt gagcgatgct acgatggaac tgccaggcac
     8701 gacacgagcg aatatcacgg ttggcttgat cccaaggttt gcggccattt ctttaactaa
     8761 tggcaggatc gcaggttcaa aaacgtcaag gtcatcgatt tcaagacccc tcatatttgc
     8821 cgtaatacca gcgcatccgc agggcagggc gaacacatca agctcgatca aaagtaccgg
     8881 cctgcctatc agttttctca tcttgtcttc aaaacccgcc ctgtttttca aaagttcttc
     8941 tgtgatcatg ttttcacctc tacccatggt aaacacttct caaatccggt tccgccattt
     9001 gaatatacct gcacgacctt ctttgtaagt gtcctgcaat aaccgcattt attgcatact
     9061 tttctgcaat ttttccattg tttttccagg ataccatcaa agcttttatt gggaatatta
     9121 tacaggtctt tcaaatcctt ggggcagtcc agaaggtcca tgagattacc atcataactt
     9181 tcattatagt aggcattgac gcaattcagt atccagtcca caaaatgcgt cctgcccccg
     9241 atcttataaa catcggttat atccctgtag agcttgagat cctcaggcct tatccagggg
     9301 gattttgtta tcaattcagg atttttgatc cttaattcaa gacatttcag gtaataataa
     9361 tcatcaagca catttggcct gggttccggc ccgaaagcat gtgagaaaaa attaaaatgc
     9421 gcatacctga atggacaacg gtaaaggcag ccttcgttta caagaagctt taattcgcaa
     9481 tccacagaat ccctgatggc atgaagaaca tcgaaatgcc ggttgacatt tgaatcgatg
     9541 actattgatt ttgcccccag gtcctcgaag aattcagctt tctgcggcga gtccacaaaa
     9601 gcaaggactg aaaccactac atccacatca aaatcccttg aaatggtctc tacaagataa
     9661 gggtctgcta caacgattga atcaatcccg atatccacca gtttttctat ataccagtgg
     9721 tttatcctga atccttcggg tgtaagctgt cgtcctccca tgcagcttga attaagaaca
     9781 agttccatct tgacgccgtt cttatgggcg aactctgtct gttcctttat atcttcaagc
     9841 tggggggaag caagattgct tcttcccgta ccgatataat caggggagcc tgccatgaaa
     9901 atagagtata tcttctcctt ctgtttcaat aattccttaa gactgtcaat gtgtcccgga
     9961 gatggaacga agagcttcat gattttccct gttagttccc attatgataa atgttttgca
    10021 ggattcttaa atcttcatgc aagggtacga cagtaccaac ctttccttca agtgccattt
    10081 ttgaaagcct gacatgactt cccgaacgac cacttatttt gaatccaaac ttattgcaga
    10141 gaatcttaat agcagtttca actgatactt ttctaagctt cgacaactgg agatacctca
    10201 aaagtagtta tgatggattt tgcttgagtc tccattggaa attcccccaa atacaactcg
    10261 gtggcttctt tgaggttatc tacagcttct tctatagttt ttccctgaat gaaagttccc
    10321 acttcaggac actctgctac atacatgtcc tcttctctgt gcaatactgc tgtgagcatt
    10381 ttcacttttg ccatgtttgt atcagtggtt ttcctattac ttaaatattt atatcgaagg
    10441 aacgcaaata atgaacaaac atcaaaatga aaagagggta gttctatggt aacaaacgac
    10501 gatttgattt ccagcaagtc aaatcctctg aacatataca ttaaatatct ttccatcctg
    10561 ctttaccgca agaatcttat ttccggattt tttcgcccat gaatcaatat tctgcggcgc
    10621 cacagggtca tccgccgtta taagtaagat ctcaccgctt ttcaattgct ccacacgttt
    10681 ttttgtaagt atgaggggat atgggcagca ttcgccacgc acatcaagtg tatcatcagg
    10741 aataatctca gacatagggt acccatttta acccatgatt atttaaaact gtctggagga
    10801 tatcatgaga atatcattat ttactaatgg aaatcagttg atttataaac ccttatatcc
    10861 ttctttaaca attctgacca gtgtgattta atgaaacaaa tagaaacagc ttcaggcatc
    10921 gggggaatgc tcacagcatt ccggaaactt gcgaggggaa ggaaaaagat aaccttcatc
    10981 ggctgccctg gctggtgcaa tccttttgct gaacttctag gctatgtttt acgagatgca
    11041 ggaaaagaga tggtatttat tccaaacctc aggaaggacc tggcggcaaa tatccaaatg
    11101 acggtttacg gtatgcagat cggtgaaaag gcggatcctt ctgctgatac tgttgtgctt
    11161 ctcggagggc ttgccatgcc caaaatggaa gtggatgtca ggggtttaaa taaaatgata
    11221 gaggagataa ccagagggca tcctgatccg caaatcctgg gcgtctgcat cggcggggta
    11281 tttcagaaag caggctggga taaattgatt gattttgatt atctcatgga tgcggatatg
    11341 gaagttgtga cctatgagtt caaataaaat taaggttcaa acaaaattat gaacaaatta
    11401 gcaagactgt ttataaaaac aagcctggtg taccttgtcc tgagcgcgat cctgggtgta
    11461 ctcataacca ttggtcccgg atacagtttc atgcattcac attttgcagt ggtggggtgg
    11521 gtatctttca tgcttttcgg gctggcttat gagagcatcc cgcgctatac cagcagggcg
    11581 attttcagta aaaaacttgg aatgatacaa ttctggcttg ccaatatcgg attgatcggc
    11641 ctttcggttt catactcctt catgagaatg tatatgttga aagaaaaaga ttattccgac
    11701 gccattactg cgttaatgat ctttggcctc atcgaagcca tttcatgttt cattttcgta
    11761 tataatatat ggaagtcaat gggaaacaca ggcacttcaa agtgaataat aatttgaagt
    11821 taataatatt caaagtaaga ggtaaattag aatggaatgg aaggcagatg caataaaaga
    11881 acttgaaaaa gtaccggaaa atgtccggaa tatggcaaaa atggggattg aaactgcggt
    11941 cgaaaagaag ggggagaata ttgtcactct caaggatgtg aatgaagccg ccgcccaatt
    12001 cagggccata atggaaaaag aggaaaaaac aacaaaaatt gcggtggtca ggtgcgatat
    12061 tgttagtgag acatgccctg gagtcgcctg ttttaaggcc ttcaataaac gaaaagtaca
    12121 tttcgagcaa tatgggaaag atgcccagat aataggattt ttcacatgcg gcggatgccc
    12181 cggcaggagg attttcaggc ttgttgacag cctcctgaaa catgatgtag atgtggttca
    12241 cctgagctca tgtatgcttc ttgagaaaag ttatgcaaaa tgccctcata ttgaggaaat
    12301 aaagcagatg ataaccgtca agggtgtaaa aatcgtag
//
LOCUS       DATGBN010000476         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_232269, whole genome shotgun sequence.
ACCESSION   DATGBN010000476 DATGBN010000000
VERSION     DATGBN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232269"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VKL21_12190"
     CDS             <1..600
                     /locus_tag="VKL21_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HMB46569.1"
                     /translation="KFEFNYIDAMDKASALSTVQIDVENAERYGIKYIDPTGAQMRPF
                     ILHCSPSGAIERCIYALLEKANMNSEKGIVPMFPVWLSPTQVRVIPVAEKHMEYAEKV
                     AGELNCRVDIDDREETVGKKIRDAGKDWIPYVAVVGDEELNSGNITVTVRSESLPNKP
                     FNVKMSIPELNERISTEISSFPFKKQPLAIKLTARPKFV"
     gene            610..1524
                     /locus_tag="VKL21_12195"
     CDS             610..1524
                     /locus_tag="VKL21_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein"
                     /protein_id="HMB46570.1"
                     /translation="MRIQTWFGIFTVENDTIITAEIFPKDLSSIIGRLLKEQLMLRGS
                     IAGSDLRDLAVEYGFAVSNNEYDSLLHELNIRLVKEQVSKTLTPDMRIIAAVEAIDDL
                     NETSNVLSERLKEWYILNYENTHLSGEELAYKIIGMEETGESAQLKIMQSFSSSLLAT
                     YETRDHIEEYIKVNMPIIAPNITNIAGHILGARLLSIAGSLQKLAFMPSSTIQVIGAN
                     NALFKHLKGKASSPKHGLIFRHPLIITSPGWQRGKIAKILSSKISLASRYDLYSGELK
                     EELSGELKLKIDAIRKQKNKKKLIRPYY"
     gene            1922..2356
                     /locus_tag="VKL21_12200"
     CDS             1922..2356
                     /locus_tag="VKL21_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HMB46571.1"
                     /translation="MVQQDNLQLMKTLDDAWNSQDWETFKKRHAENAAIFWPGQPDPT
                     KGRKAHHDEAVEFFKTFPDNHIVNNPYKILFGQGDYTCSVAEFTGTMKGSMKGPGGKI
                     IPPTNKKFRVEFCTVAHWKNGEIVEEKLFYDLMGLMRQIGLG"
     gene            2437..>2736
                     /locus_tag="VKL21_12205"
     CDS             2437..>2736
                     /locus_tag="VKL21_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMB46572.1"
                     /translation="MLFPIPGEYTIDPMHSFTCFIAQHLVVGQVWGCFDSIKGKITMA
                     DDPTLSSVDISIDTASISTHNERRDDDIRSARFLDVKKFPTMTYHSSGVIAEPRGH"
BASE COUNT          917 a          556 c          590 g          673 t
ORIGIN      
        1 aagtttgaat tcaattacat agacgctatg gataaggcaa gcgcgttatc cactgtgcag
       61 atagatgttg agaacgcaga acgttacgga ataaagtaca tcgatccaac aggcgcgcaa
      121 atgagacctt ttatcctgca ctgctcacca agcggcgcga tcgaaagatg catctatgcg
      181 cttcttgaaa aagccaacat gaactcggaa aaaggaattg tccccatgtt cccggtctgg
      241 ctttcaccta cccaggtgag agtgattcct gttgctgaaa agcacatgga gtatgcagaa
      301 aaagtagcgg gagaattgaa ctgcagggtc gacattgatg atcgcgaaga aaccgttgga
      361 aagaagatcc gcgatgccgg aaaagattgg ataccgtatg tagccgtggt tggggatgag
      421 gaattaaata gcggaaatat taccgtaacg gtcagaagcg aatctttgcc caacaaaccc
      481 tttaatgtaa agatgagtat tccggaactc aacgaaagga tatcaacaga aatatccagt
      541 ttcccattca agaaacaacc gcttgccata aaattgacag caagaccaaa attcgtttaa
      601 gataagcaga tgcgaattca aacatggttc gggattttca ctgttgaaaa tgatacgatt
      661 atcaccgcag aaatattccc gaaagacctt tcttccatta ttggacgact ccttaaggaa
      721 caattgatgt taagaggcag tattgcagga tcggatcttc gtgacctggc agtagagtat
      781 ggttttgcag tttcgaataa cgaatatgac agcctgcttc atgaactgaa tatcaggctg
      841 gtaaaagaac aggtctcaaa aaccctgaca ccggacatga gaataattgc ggcggtggaa
      901 gctatcgatg acctcaatga aacaagcaac gtcctttcag aaagacttaa ggaatggtat
      961 attctgaatt atgaaaatac gcatctatca ggagaagaac ttgcctacaa gataatcggg
     1021 atggaagaaa caggagaaag tgcacagttg aaaataatgc aaagtttttc ttcaagtctt
     1081 cttgcaacgt atgaaacgcg agatcatatc gaagaataca tcaaagttaa catgccgatc
     1141 atagcgccaa atataaccaa tatcgcagga catattcttg gggcgcgcct cctgagtatt
     1201 gcaggaagtc tacaaaaact tgctttcatg ccttcaagca ccatacaggt catcggggct
     1261 aacaatgcgc tttttaaaca cctgaaagga aaagcctcct cgccaaaaca cggactcatt
     1321 ttcaggcatc ccctcattat tacctcaccg ggatggcagc gcggaaaaat tgcaaaaata
     1381 ctctcatcga agatctccct tgcttcacgg tatgatcttt attcgggtga gttaaaagaa
     1441 gagctgtccg gggaactgaa attaaaaatt gacgccatca ggaagcagaa gaataagaag
     1501 aaattgatac gaccgtacta ttaaaacttt cgttcaacat tagctttata tcgctggggt
     1561 tgcgacccca gaccccaacg gttgcgccgc cgctggcggg gttttataag atatccccgg
     1621 ttgcatataa tcacaatagc caataattag aaaaaaacac cacctgaaag aaaaattact
     1681 caatgacaat tgatgataaa ggaaatacaa cagaatattc ttagaataac aaaatgttat
     1741 tctgctaata ttacccacga aaataagcaa gtttcaattc tcttcaaata tttcaagcat
     1801 acaatatgac ttaactaaat ttttagtgca actacgcatg caggtttaca cattggagtc
     1861 gaaaagacca taattcttat ataccatgca cccccatgaa attatgggag tgcaaaatat
     1921 tatggtacaa caagataacc ttcaattaat gaaaacttta gatgatgcat ggaattctca
     1981 ggattgggaa acattcaaaa aacgtcatgc tgagaatgcc gccatattct ggccaggtca
     2041 accagaccca actaagggac gaaaggcaca tcacgatgag gcggtggagt tttttaagac
     2101 atttcctgat aaccatatcg tgaacaatcc gtataagatt ctttttggtc agggcgacta
     2161 cacctgctca gtagctgaat ttacaggcac aatgaaagga tccatgaagg gtcctggtgg
     2221 caaaataatt ccacccacga acaaaaaatt tcgggtagaa ttttgcacgg tcgcccactg
     2281 gaaaaatggg gaaatcgtcg aggagaagct cttctacgat ctgatgggat tgatgaggca
     2341 gattgggctg ggctaaaccg ggatactgca tcaacaggtt aataacaata tatccctatg
     2401 aaccagacaa aaaatacatt acggattttt aatggaatgc tttttccgat acctggggag
     2461 tatactattg accctatgca tagcttcact tgctttatag ctcaacatct tgtagtcggg
     2521 caggtctggg gatgttttga ctcgataaaa ggtaaaatca ctatggcaga cgatccaaca
     2581 ctgtcatcag tagatatcag tattgacaca gcgagtatca gcacccacaa cgagagacgt
     2641 gatgatgata ttcgtagcgc acgatttctg gatgtgaaga agtttccaac aatgacctat
     2701 catagcagtg gtgttatcgc tgaaccaaga gggcat
//
LOCUS       DATGBN010000477         7079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_503594, whole genome shotgun sequence.
ACCESSION   DATGBN010000477 DATGBN010000000
VERSION     DATGBN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503594"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /locus_tag="VKL21_12210"
     CDS             <1..2174
                     /locus_tag="VKL21_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB46573.1"
                     /translation="NSITNARTIILALSISSFLIAILFGVLISSQILKPINRINDAIS
                     KVRGGELDTRIKIESWDEIGELSSSFNTMTWDLRHEQELLIQSEDKFRTLYESSSDSV
                     ILLDETGFFDCNESTLRIFGFADKESFIKLHPNDISPPYQPDGVDSMKAANNKIAQAF
                     KNGSNKFEWMHRRKNGEDFYAEVLLTVFWLKDKKVIQATVRDITERKAFLDAIIYSNE
                     FSKTILNSMKEVISIIDVTNFKIIEANDAFLKGYGLKKEDVLGKKCYEITHQAEIPCN
                     SPEEMCPLKETMKTGMPSSCEHIHHGKNGEKIFVEITASPIIDKMGKVINIIHAARDI
                     TDRKLAEETLSDSEEKFRLTVQSASDAIFITDIEHRIISLNKSAINMFGYIEKEVIRK
                     SFHILLPERYKETYQYGLEWMLEPDDTGIINKSIEMHGLRKDGTEIAIDLSAASWKTT
                     TGTYQASIVRDITDRKRMEEEIIRINMELKIADKIKTQFLSVISHELRTPLTPMKAQL
                     QMNLAGYFGSIAEKQKSSLEMILRNTERLDRMIVDVLDISKLEAGVMKFSKDKANLNI
                     LVRNAIETMMQKAEDKKIKLNLKEESVPDIIMDTDRITQVIMNLLNNAIKFTYPGGSI
                     YVEVSCNHMNAHVKVKDTGVGIMKEDQGKLFQPFVQVDSTETRKFEGTGLGLSICKGI
                     INNHGGKIWLESEFGKGSTFQFTIPIYREETEKDTQVNLFK"
     gene            2194..2559
                     /locus_tag="VKL21_12215"
     CDS             2194..2559
                     /locus_tag="VKL21_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMB46574.1"
                     /translation="MAKIMIVDDNPDISETLKTIMEIDNHVTETAFNGEDFLNKVEKA
                     KPDIVLLDVMMPGLKTKEILAKLKEKGLGNLKIILVTVVRFSEEERKALMSENNIRDY
                     VTKPFDVPDIMKRVRKQLS"
     gene            2570..3175
                     /locus_tag="VKL21_12220"
     CDS             2570..3175
                     /locus_tag="VKL21_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46575.1"
                     /translation="MTDDNAVVAVNYWKETRKELKKLLSEPWKTLLLIHNSTDVKEIA
                     DFIKKLSDSKKIVYISLTRTDNAIIPSFKDMDKSLFIVDCISSGVFEKKNTKTSFYEN
                     SPSTFDEIIALNEKYLNELKPDLVILDSLSQFIDFSTPTPKYTLDLYKYLNSLKNIRT
                     PCRFILLYMDSRSKDVVFMPTYDVDLILRLEVIVEKIYWHG"
     gene            complement(3206..5758)
                     /locus_tag="VKL21_12225"
     CDS             complement(3206..5758)
                     /locus_tag="VKL21_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMB46576.1"
                     /translation="MTEKSFKLTIGKKIFLGFSILIVIYLGMALHSYYDLKEISGLAE
                     NAVPLSSRIISLQEFAISLESLEKDIDKFFTVNYKENEEEANKEFKQMHSIIWSLKND
                     TDSKSMDGLLEMERILSEIQSNFNYLAKIEQNSANSREINEKRFLIYELINSERQKHR
                     ELLLETTNKIQVNVLDQQRGISGIIKEFLMLGISILVFGVLLSIITSRSISRPIEKLR
                     AATTEIGQGRLNINIPIQSNDEVGQLASAFNKMTGELQKTTVSKDYVENIIRSMFDTL
                     VVTAPDGTIQTVNKAICDLLGYRPEELLGQSILMILSDENPESKSSNIKEILKNGSIH
                     NMEKEFLAKDGRKIPILLSISSMCDNERNILGLVCVARDITELKRTEDELCELYQFNQ
                     EVISGAGEGITVYDLEFRYMLWNKFMETMTGVLQEDVLGKKALDVFPHLREQGIDLLL
                     EKAINGKTVSSNDTPYKIPGTDKSGWVNATYAPHRDANGKIVGVIATVRDITKRKKAE
                     DLLNKSKEFAETVINSMKDAIAIIDVNTFRIINANSYFLEIYGMREEELIGKTCYEIT
                     HKRARPCTPPDDICPLMDTLNTGKYSTAEHVHYTKSGEKRYVEVSTTPVKDEFGKVVT
                     VIHVARDITERKVAEDKIKASLKEKETLLKEIHHRVKNNLQIVSSLLDHQSQFIKDKK
                     VIEIFTDSQNRIASMALVHEKLYQSKDLGKIDFSDYINDLAANLFQSYIENSGKIRLN
                     MKIENIQLDIDFAIPCGLIINELVTNSLKYAFPAGSVGEIKIVFRKTIENMLELVISD
                     NGVGIPPDLDFRKTGSLGLHLVTILAENQLHGKINLNRNEGTEFKIEFKGIK"
     gene            complement(5769..6779)
                     /locus_tag="VKL21_12230"
     CDS             complement(5769..6779)
                     /locus_tag="VKL21_12230"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HMB46577.1"
                     /translation="MKKRASLVLLGVILLAIFSGCTSTQSQTKNIVVSEKPIQTYEGK
                     KVLYVDSYHAGYEWSDGITRGIENIFNNTGVELKILRMDTKRNDTEEFGKQAGLMAKS
                     AIEDFKPDVVIISDDPAFKYLLMPYYRDAALPFVFCGINWDASIYGAPYNNTAGMIEV
                     SLTPKLITFLKEYSRGERLGFIAGNTSTDRKNAQYYEKLYNFTFTKKYQVNTFEEWKK
                     SFLGLQEEVDVLILENNAGITNWNDREAEAFVLENTKIPAGAVNIWLSNYSFISLTKI
                     PEEQGEWSANMALIILNGTHPSDIPLTTNKKGKIIVNLKIANKLGVIIKPDLLKNAEI
                     IR"
     gene            complement(6803..>7079)
                     /locus_tag="VKL21_12235"
     CDS             complement(6803..>7079)
                     /locus_tag="VKL21_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMB46578.1"
                     /translation="LMYSNIQTVFRFLHVFTGRIKSIGGLGIYLIESGMHDEQAIATL
                     KQLFDGIIEIRSENDTNFIRVVGLSSKPTPWFEYEIEGAKLKIVGRK"
BASE COUNT         2214 a         1374 c         1296 g         2195 t
ORIGIN      
        1 aaaattcaat aaccaatgca agaactataa tcctcgcatt aagcatctcc tctttcctca
       61 tagcgatcct atttggcgtt cttatatcca gtcaaatttt gaaaccgata aaccgaataa
      121 atgacgctat ttcaaaagta agggggggag aacttgatac caggatcaaa atagaatcct
      181 gggacgagat cggagagctt tcttcttctt tcaatacgat gacgtgggat ttaagacacg
      241 aacaagaatt attgatccaa tctgaagata aattccgcac cctttatgaa agttcatcag
      301 attcagtcat tctgcttgac gagacgggat ttttcgactg caatgagtcg acgcttcgga
      361 tattcggctt tgcggataaa gaaagtttca taaaattgca tccaaacgat atatccccac
      421 cataccagcc tgatggcgtg gattcaatga aagctgctaa caacaagatc gcgcaagcct
      481 tcaagaatgg ttccaacaaa tttgaatgga tgcacaggag aaagaatggc gaagattttt
      541 acgctgaagt attgctgaca gtattctggc taaaggacaa aaaagttatt caggcaacag
      601 tcagggacat cacagaacgc aaggcatttc ttgatgcgat aatttattca aatgaatttt
      661 caaagacgat tttgaacagt atgaaagaag taatttccat tatagatgta acgaatttta
      721 agatcattga agcaaatgat gctttcttga aaggatacgg cctgaaaaaa gaggatgttc
      781 tcggaaagaa atgctatgag attacacatc aagctgaaat accatgcaat tccccggaag
      841 aaatgtgccc gctaaaggaa acaatgaaga caggcatgcc ctcctcttgt gaacatattc
      901 atcatggaaa aaatggagaa aaaatatttg ttgaaatcac tgcatccccg ataatagata
      961 agatgggaaa agtcatcaat ataatccatg cggcaagaga catcactgat cgtaagcttg
     1021 cagaggaaac ccttagtgac agtgaagaaa aattccgttt gacagttcag tccgcaagcg
     1081 atgccatctt tataacagac attgagcatc gcataatttc ccttaacaaa agtgcaataa
     1141 atatgttcgg ctatatagag aaggaagtaa tcaggaaatc atttcatatt cttttacctg
     1201 aacgttataa agaaacttat caatatggac ttgaatggat gttagaaccg gatgacacag
     1261 gtataatcaa taaatccata gaaatgcacg gtttgaggaa agatgggacg gagatcgcca
     1321 tcgatttatc cgcagcatca tggaaaacca caacgggaac atatcaagcc tccatagtgc
     1381 gcgatattac tgacagaaaa cggatggagg aggaaattat aagaataaat atggagctaa
     1441 aaatcgcaga taagatcaaa acacagttcc tgagcgttat ctcgcatgag cttcgcactc
     1501 ctttgactcc catgaaagca cagcttcaga tgaatcttgc gggatacttc ggatcgatcg
     1561 ccgaaaaaca aaaatcaagc cttgaaatga tcctgcgaaa cactgaaaga cttgaccgga
     1621 tgatagtgga tgttcttgat atttccaaac tggaagcagg agtaatgaaa ttcagcaagg
     1681 ataaggcaaa tctcaatatt cttgtcagga atgcgataga aaccatgatg caaaaagctg
     1741 aagataaaaa gatcaaactc aatttgaagg aagaaagtgt tccggatata ataatggata
     1801 cagacaggat cacccaggtt ataatgaacc tgttaaataa cgctattaag ttcacatatc
     1861 ccggcggctc aatatatgtg gaggtatcat gcaatcacat gaatgcgcat gtaaaggtga
     1921 aagatacagg ggtcgggatc atgaaagaag accagggaaa gttattccag ccctttgtcc
     1981 aggtggattc aactgaaaca aggaaatttg aaggtacggg tctgggcctt tccatatgca
     2041 aagggataat caataaccac gggggaaaaa tatggttaga aagcgagttt ggtaaaggat
     2101 caacattcca gttcacgatc cccatttata gagaagaaac tgaaaaggat acacaggtta
     2161 atttatttaa ataattatga agatgtgata catatggcaa aaataatgat agttgatgac
     2221 aaccccgata tatcagaaac gttaaaaaca ataatggaaa ttgacaacca tgttactgag
     2281 acagctttta atggagagga tttcctgaac aaggttgaaa aagcaaaacc tgacattgtt
     2341 cttctcgatg taatgatgcc tggattgaaa actaaagaaa tacttgcaaa gttgaaagaa
     2401 aaagggcttg gaaacctgaa aataatactt gtcacggtgg tcaggttctc agaagaagaa
     2461 cgcaaagcct tgatgagtga aaacaatatc agggattatg ttacaaaacc cttcgatgta
     2521 cctgatatca tgaaacgggt aagaaaacaa ttaagttagt aaggtagtaa tgacagatga
     2581 taatgcagta gtcgctgtaa actactggaa ggaaaccagg aaagaattaa agaaattgct
     2641 atctgagcca tggaaaactt tacttcttat ccacaattcc acagatgtta aagaaatagc
     2701 ggattttata aagaaattat ccgattcaaa aaagatagtt tatatctcgc tgacgagaac
     2761 agacaatgca attatccctt cttttaaaga tatggataaa tcattgttca tagtagattg
     2821 catttcatcc ggagtttttg aaaagaaaaa cacaaaaaca agtttttatg agaattcacc
     2881 ttcaactttt gatgaaataa ttgcattgaa tgaaaaatat ctgaacgaac tcaaacccga
     2941 tctggtaatc cttgattcat tatcgcaatt tatcgacttt tccacaccga ctccaaaata
     3001 tacacttgat ttatataaat atttgaatag cttaaaaaat atcaggactc catgcaggtt
     3061 cattctatta tatatggatt cgcgttcaaa agacgtagtt ttcatgccta catacgatgt
     3121 tgatctgatc ttaaggctgg aagtaattgt tgaaaagata tactggcatg gatgaggttt
     3181 tcatgtttca ctgttttctc ttgcgtcatt ttatcccttt aaattcaatt ttaaattctg
     3241 ttccttcatt tcgatttaga ttgattttcc catgaagctg gttttctgcc agtattgtga
     3301 ccaggtgtaa acccaatgac cctgtttttc taaaatccag atccggaggt atgccaacgc
     3361 cattatcact tattacaagt tcaagcatat tttcaattgt tttgcgaaac actatcttaa
     3421 tttcaccaac gctacctgct ggaaatgcat atttcaaaga atttgtgacc agttcattga
     3481 ttatcaatcc gcatggaatg gcaaaatcaa tatccagttg aatgttttcg attttcatat
     3541 taagtcttat tttgccggaa ttttctatat aagactgaaa aaggttagcc gctaaatcat
     3601 tgatgtaatc ggagaagtcg atttttccca ggtcttttga ttgataaagt ttttcatgca
     3661 caagagccat ggatgctatc ctgttctggc tgtctgtaaa tatttcgatc acttttttat
     3721 ctttgatgaa ctggctttga tgatcaagaa gactggaaac gatttgcaaa ttgttcttaa
     3781 cacgatggtg gatttctttc agaagcgttt ctttctcctt aagagatgcc ttaattttat
     3841 cctcagccac cttacgttct gttatatccc ttgctacatg aatgacagtg acgacttttc
     3901 caaattcatc ttttacgggt gttgtggaga cctctacata tcgtttttct ccactcttcg
     3961 tataatgcac atgctcagct gttgaatatt tacccgtatt caaagtgtcc atgagagggc
     4021 agatatcatc cggtggagta cacggccggg cacgtttatg ggtaatctcg taacaggttt
     4081 ttcctatcaa ctcttcttcc ctcatcccat aaatctccag gaaatatgaa tttgcattga
     4141 taatcctgaa agtattgaca tcaatgatag cgatagcatc tttcatacta tttatcactg
     4201 tttcggcaaa ttcttttgat ttatttagta agtcttccgc ttttttgcgt ttggtgatat
     4261 cgcgcaccgt agcaatgacc ccaacgattt ttccattggc atcccggtga ggtgcgtacg
     4321 tcgcgtttac ccagcctgat ttatcagtcc ctggaatttt ataaggagta tcattggatg
     4381 ataccgtttt tccgtttatc gctttctcca gaaggaggtc aatgccctgt tcccgaagat
     4441 gcgggaatac atctaaagct tttttcccaa gcacatcttc ctgcaagact cctgtcatag
     4501 tctccatgaa tttattccaa agcatatacc gaaattcaag gtcatacact gtaatccctt
     4561 caccagcgcc cgaaatgact tcctgattga attggtataa ttcacacagt tcatcctctg
     4621 tccgctttag ttcagtaatg tccctggcta cgcacacaag cccaaggatg tttctttcat
     4681 tatcgcacat ggacgatatc gataataata tgggtatttt tctgccatct tttgcaagga
     4741 attctttttc catattatgg atcgaaccat ttttcaatat ctccttgata tttgaacttt
     4801 tagactctgg attttcatca gaaagtatca taagaataga ttgccctaaa agctcttctg
     4861 gtctatatcc cagcaggtca cagatggctt tatttactgt ctggatagtg ccatccgggg
     4921 cagtgacaac cagcgtatcg aacatacttc taataatatt ctccacataa tcttttgaaa
     4981 cagttgtttt ctggagctct ccggtcattt tattgaaagc agaagccaat tgccctactt
     5041 catcatttga ttgaattgga atatttatgt ttaatcgccc ctgaccgatt tcggtggtgg
     5101 ctgcccttaa tttctctatt ggccgggaaa tggatcttga agtaataata gaaagcagta
     5161 ctccgaatac caggatagag atacccagca ttagaaattc tttgattatt cctgaaatgc
     5221 ctctctgttg atccagaaca ttaacctgaa ttttatttgt ggtttccaat aacaattccc
     5281 ggtgcttttg cctctcgcta tttatcaact catatatcaa aaatcttttt tcatttattt
     5341 ctctggaatt ggcactattt tgttcaattt tggccaaata attgaaatta ctctggattt
     5401 cagataatat tctttccatc tcaagtaatc catccattga tttactatct gtatcgtttt
     5461 ttaatgacca gataattgaa tgcatttgct taaactcttt attagcttct tcttcatttt
     5521 ctttataatt caccgtaaaa aatttatcta tgtccttttc cagagattcc agagaaatag
     5581 caaattcctg caaagaaatg atccttgagc ttaatggcac tgcattttct gcaagaccgc
     5641 ttatttcttt caagtcataa taactatgca gcgccatacc cagataaata acaataagaa
     5701 tggagaaacc cagaaaaatt ttttttccaa tagttaattt gaaggatttt tcagtcatat
     5761 tctaaaaatt agcgaataat ttcagcattt ttcagtagat ctggtttgat aattacaccc
     5821 agcttatttg cgatcttcag attcacgata atcttgcctt tcttgttcgt tgtaagtgga
     5881 atatccgagg ggtgggtacc gttaagaatt attaaagcca tatttgcaga ccattcaccc
     5941 tgttcttccg gtatttttgt caaagagata aatgaataat tggacagcca gatattaacg
     6001 gctccagccg gaatctttgt gttttcaaga acaaatgctt ccgcttcacg atcattccag
     6061 tttgttatgc cggcattatt ttcaagtata agcacgtcaa cttcttcctg aagacccaga
     6121 aaagactttt tccattcttc aaatgtgttc acttgatatt ttttagtaaa agtgaaatta
     6181 tacaattttt cgtaatattg ggcatttttc ctgtcagtac ttgtatttcc cgcaataaat
     6241 cccagcctct cacctctcga atattctttt aagaacgtga taagcttcgg ggtcaagctt
     6301 acttctatca taccggcagt attgttataa ggcgcgccgt agatcgaagc atcccagttt
     6361 atgccgcaga aaacaaaggg cagggctgca tccctgtaat acggcataag gagatactta
     6421 aaagccgggt catcagagat aataacaaca tcaggcttga aatcttcaat agcagatttt
     6481 gccattaatc cagcctgttt tccaaattcc tccgtgtcat ttctttttgt atccattctc
     6541 aggatcttga gttcaacgcc tgtattgtta aagatgtttt caattcctct tgtaataccg
     6601 tcgctccatt cataacctgc atgataggaa tctacataaa gaactttttt gccttcataa
     6661 gtttggatag gcttttcact gacaacgata ttctttgtct ggctttgagt tgaagtgcag
     6721 ccggaaaata ttgcgagcaa gatcacaccc agcaatacca gacttgctct ctttttcata
     6781 tttttcctcc atttggcgcc agttattttc tccccactat cttcagtttt gcaccttcga
     6841 tctcatattc aaaccatgga gtcggttttg aggacaggcc caccaccctt ataaaattcg
     6901 tatcgttttc cgatctaatt tctattatgc cgtcaaatag ttgttttaat gtcgctattg
     6961 cctgttcgtc atgcatgccg ctttctatca gatatattcc aagacctccg attgatttta
     7021 tccgtcctgt aaaaacatgg aggaacctga aaactgtctg gatatttgaa tacattaaa
//
LOCUS       DATGBN010000478         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_232505, whole genome shotgun sequence.
ACCESSION   DATGBN010000478 DATGBN010000000
VERSION     DATGBN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232505"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..609)
                     /locus_tag="VKL21_12240"
     CDS             complement(109..609)
                     /locus_tag="VKL21_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMB46579.1"
                     /translation="MICNEVLDCIRARRSTRHFQEGQIEEEYLETLLEAAIWAPNGGN
                     NQSWLFTAIQNKDILSKINELLREGLQHWTPDDDYPGKLGAKAYAQRKDCHFFHHAPT
                     LIIASNRPNYENAMADCALALENLFLAAQSLRLGSCYINSLHWLRNDPELRNYLFQLE
                     ALKLGL"
     gene            846..1142
                     /locus_tag="VKL21_12245"
     CDS             846..1142
                     /locus_tag="VKL21_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMB46580.1"
                     /translation="MENASQHKELFEKIFSFLEKEGATKVAVFGSYARGEEKPESDID
                     ILVEFSENKSLLTLVRIERELSEFLGVKVDLLTEESISPYLIDGIKREAKVISA"
     gene            1139..1489
                     /locus_tag="VKL21_12250"
     CDS             1139..1489
                     /locus_tag="VKL21_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMB46581.1"
                     /translation="MKKNDSVYLRHILNSIERIEDYTEGMVKDDFLSSNLVQDGTIRQ
                     IEIIGEATKNLSKNIREKYSQISWSDIAGMRDRLIHHYFGVNLNAVWDTVKIDLPILK
                     DEILVILDVLETEK"
BASE COUNT          531 a          287 c          326 g          497 t
ORIGIN      
        1 actatacttg ttttactcac aaaacgagat gttttgaact atttttatat aaatgtacgg
       61 atgaatttgc gtgctaagca ataacgaaaa aatgaatagg tttataaatc aaagtcctaa
      121 tttgagagcc tccaactgaa aaaggtaatt gcgtaattcg ggatcatttc gcaaccagtg
      181 cagagaattg atgtagcagc tacctaatct caacgactgc gccgctaaaa agaggttttc
      241 aagtgcaagc gcacagtctg ccatagcatt ttcataatta ggccggttag aggcaatgat
      301 gagtgtaggt gcatggtgga aaaaatgaca gtctttcctt tgtgcataag ctttggctcc
      361 gagtttaccg ggatagtcgt catcaggagt ccaatgttga agcccctccc gcagcagttc
      421 gttgattttt gaaaggatat ctttgttctg gattgcggta aacagccagc tttgattgtt
      481 acctccattg ggtgcccata tggcggcttc aagtagcgtt tccagatatt cctcttcaat
      541 ctgtccttcc tggaaatgac gtgtacttct ccgtgcacga atacagtcaa gtacttcatt
      601 acaaatcata atttgttctc cttttcacaa ccggaagata tccggctaca aatcaacgca
      661 atgtctttaa ttatactaca atatttcata aatacaatgc gccggcacaa cccggaataa
      721 agcattagag atattgtaga aatgtctgga atgaaccaag ccacgttgtg ggattttatg
      781 tttcttaact ggctgctcct tcatttaaga tacgtttaaa ttataacaaa tgaaattata
      841 ccgacatgga aaatgcttcc cagcacaaag aattgttcga aaaaatcttt tctttccttg
      901 aaaaagaagg agcaacaaaa gtagctgttt ttggctctta tgcaagggga gaagaaaagc
      961 cagaaagcga tatagacatt cttgtagaat tttccgaaaa taaaagtcta ctcactttag
     1021 tcaggatcga gagagaattg tcagagtttc ttggggtaaa agtcgattta cttaccgagg
     1081 agtcaataag tccgtacctt attgacggaa tcaaaagaga agcaaaagtg atctcagcat
     1141 gaaaaagaat gattcggttt acctcagaca catcctcaat tcaattgagc gcattgaaga
     1201 ttatactgaa ggtatggtaa aagatgattt tttatctagc aatctagttc aggacgggac
     1261 gatcaggcaa attgagatca taggtgaagc taccaagaat ttatcaaaga atattcggga
     1321 gaaatattct caaatttctt ggagtgatat tgcaggtatg cgagatagat taatccatca
     1381 ctattttgga gttaacctta atgcagtatg ggacactgtg aaaattgatc tcccgatttt
     1441 gaaagacgag attctggtta tacttgatgt tctggagact gaaaaataag ctgagttttc
     1501 aacactgttt tatcctgaaa ttatttcact ttgaaactca tagtaagttt catatgaact
     1561 aattttataa aattaaagat tacagctttt ttccgtgatc aagatacatt aaaagaagta
     1621 cctctgtgtt tatcatagaa t
//
LOCUS       DATGBN010000479         5801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_76761, whole genome shotgun sequence.
ACCESSION   DATGBN010000479 DATGBN010000000
VERSION     DATGBN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76761"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..378)
                     /locus_tag="VKL21_12255"
     CDS             complement(229..378)
                     /locus_tag="VKL21_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46582.1"
                     /translation="MIEMKKKKQNLIEKPSDNTKSNSMGDEPMEYEQIDRELYMFMRG
                     GFSAK"
     gene            463..1464
                     /locus_tag="VKL21_12260"
     CDS             463..1464
                     /locus_tag="VKL21_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HMB46583.1"
                     /translation="METRKVYVSGGSTYVISLPKKWVKQVNLKAGDSLVVTEHGSSLQ
                     IESSIIEKESRTKEIRISQVKSSEALERLLIAFYLVGYDTINIKLDRKDHLAYRESIR
                     KVLDYLIGVEIVEDTNEIMTLEIMLEYKRLNTRGILQRMFSIDKSMLLDLGRAMKNSD
                     INLAKDVIAREKEIDRLYFLVVRQLKSAVEFQQIAEKLGIESQRDCLGYRIVVKVLER
                     IADHIENMAKSYIQLSEIPNEIQLDEYINLINNVATTFDKSIQALFTRNDEMAELVFQ
                     ELKKIEKNYSDISKELFNIEDIQSAILEKTMLDSLGRIANYSGDIAEVAINMSKEVY"
     gene            complement(1554..3569)
                     /gene="cooS"
                     /locus_tag="VKL21_12265"
     CDS             complement(1554..3569)
                     /gene="cooS"
                     /locus_tag="VKL21_12265"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HMB46584.1"
                     /translation="MKEETQKMDDATNEMIKHAHVLGIETIFDRNGNYNTGECKKAKC
                     NFGSRGICCKQCALGPCRISGRSLKGICGASADTIAARNLLMMTGRGTAAHASHALHV
                     ATTLLKTARKKTPYKIKESIKLESIARKINIGKENGIEDMACATASAAIADILGDEDH
                     MRFAESYYPADIVGNLSNLGVMPASAGRELLDSGHETSMGTMADPKEFVLHAARLGVA
                     DISSLIISSELQDVLFGNPKLFSSKIGFNVLEKDKVNIVIHGHVPLLSEKIIEFSEDA
                     QLLEKARALGANGINVVGCCCTGNDALMRHGIPIAGSNIHQELIIATGLAEAIVVDVQ
                     CIYPNIENVARYFHTRIISTMKEARFQGAQHIPFHEENADDTAKRILDCAIENFPNRG
                     KNIFLPQEKTHDLMAGFSVENVLDVLAKMNPQDPFKPLIDSITSGDIYGIVLMAGCIS
                     PGTIESSQVIIAKELLKKNVLVFSTGCSAQSCARGGLLTPEATMEYAGDKLKGVLKAL
                     GEFSGKQLPPVWHFGSCVDNSRVILLAIELAKNMGLPLKDMPIAVSASDWIAEKAVAI
                     GTGAVALGITVHLGRAPPILGGPEVVDLLTRKSEELFGAKFIVEEDPIKASQLLLLHI
                     GNAREKLGLTSNRSGISHNSNAKDNEDILIYPEELINPQNPLLLPTY"
     gene            complement(3752..5071)
                     /locus_tag="VKL21_12270"
     CDS             complement(3752..5071)
                     /locus_tag="VKL21_12270"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMB46585.1"
                     /translation="MNKEILVEWNPHWKEEKELKLVERELLKDIEQWLDRKEILGFIG
                     VRRSGKTTIMKILISRLSSTIPQKNILFVKCDDDRVQKENLIDDAIKSYKELINPEGK
                     IFVLIDEVQEVDKWENTLKRIYDLEKDMKLIISGSNFSMLKEDFSYSLAGRIAYFEVY
                     PLSFREFLKTRLTIKDKISAFSKKDEIKHHLFEYMELGGFPEVVLENNPDIKKQLLQF
                     YFDTIIYRDIIKKRGIRNAVKMEKMVNLLLQNISNTINFSKVGKNISLSVDTVGEYAA
                     NLKDAYLVFNVPIFSFSVKAQEINPKKIYCIDSGIRNIKGFRFSLDYGRIAENIVFIE
                     LKRRNFSNPLSRIFYWHDKNQRETDFLLMNEFKISEAIQVCWDISQSEVKEREVEGLL
                     CALNEFDLESGLIITEDYEGEEIIGDKKITYLPLWFWLMFPENAINQ"
     gene            5201..>5801
                     /locus_tag="VKL21_12275"
     CDS             5201..>5801
                     /locus_tag="VKL21_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46586.1"
                     /translation="MSLILIAIGGNAVLNPEKGNPEEQQEKIDETSREITRIIREGND
                     VVLTHGNGPQIGNILAMQEECGLVHPQPLDICGAQTQGMLGYLLQRSLSNMFREIGIK
                     KQVATIITQVIVDGSCDSFHNPTKPIGLCYPEGRAKTMMNAGVKMIHDRKGYRRVVPS
                     PMPIKIVEEDVIKKLISEGVLVITAGGGDIPVMERNGVLE"
BASE COUNT         1680 a         1200 c         1157 g         1764 t
ORIGIN      
        1 tccttaaaga atcgggctcc atagattacg ccatgaataa ggcgttcggg tttgtggatg
       61 aaggtaaagc tgcgcttgct atccttccgg attcagaagc caagaggacg cttatagaac
      121 ttgccgatta tatgatcgag aggaagtatt aaattatagt tttcggctac agcaatattt
      181 aaaatcccca catcagaata tcgattgtat tcatgatttc aactcctctt attttgcact
      241 aaacccgccg cgcatgaaca tgtagagttc tctgtcaatc tgttcatatt ccatgggttc
      301 atctcccatc gaattacttt ttgtattatc agatggcttt tctatcaaat tttgtttttt
      361 ctttttcatt tctatcacga attaatattt ctcatgtaaa gttatatatt ttatctcaat
      421 aatctatata tactatcgaa tatcagtata aaacaggata ccatggaaac acgaaaagtc
      481 tacgtaagcg gcggctctac ctatgtaata tccctgccaa agaagtgggt caagcaggtg
      541 aacctgaaag caggagactc gctggtggta acagaacatg gcagttcgtt acagatcgag
      601 tcaagtataa ttgaaaaaga atcacggact aaggaaatca ggatatcaca ggtgaaatcg
      661 agcgaagccc ttgagcgtct attgatcgct ttttacctgg tgggttacga tactattaac
      721 ataaaacttg acaggaagga tcatcttgca tacagggaga gcatacgcaa ggtactggac
      781 tatctgatcg gtgttgaaat tgtggaggat actaacgaaa taatgaccct tgagataatg
      841 ctggaataca aacgtttgaa tacccgcggg atactccaga gaatgttttc catagacaag
      901 tcaatgcttc tcgatcttgg cagggctatg aaaaattcgg acatcaatct ggcaaaagat
      961 gtgatagcaa gagagaaaga aatcgacagg ctttatttcc tggttgtgag gcagcttaag
     1021 agcgctgtgg aattccagca gatagctgaa aaactcggga tagaatccca gagggattgc
     1081 cttggataca ggatcgtggt gaaagttctt gagagaattg cagaccacat agaaaacatg
     1141 gcaaaaagtt atattcagtt atctgagatc ccaaacgaaa tacagttgga tgaatatata
     1201 aatctcatta ataatgtggc tacaacgttc gataaatcca tccaggcatt atttaccagg
     1261 aatgatgaaa tggctgaact ggttttccag gaattgaaaa agatcgaaaa aaattattcc
     1321 gatatttcaa aagaattgtt caatatagaa gatatccaat ctgcgatttt agagaaaaca
     1381 atgcttgaca gccttggaag aatagctaac tacagcgggg atatcgcaga ggtagccatc
     1441 aatatgtcta aagaagtata ttgaatcaaa aaatatccaa tcggctaccc tgccaaccag
     1501 gatagctccg gaagaataac aagctacaac ggggatatcg ctttagatcg ctattaatat
     1561 gttggcagga gcaatggatt ttgagggtta atgagttctt caggatagat caaaatatcc
     1621 tcattgtctt ttgcatttga attatgagat atacctgatc tatttgaagt cagacccaat
     1681 ttttcccttg cattcccaat atgcaaaagg agcagttgtg atgcttttat cgggtcttct
     1741 tcaacaatga actttgcgcc aaagagttcc tctgattttc gagtcagtaa atccacaact
     1801 tcgggccctc ccaggatggg cggcgctcta cccaggtgga ctgtaatacc cagggcgacg
     1861 gcgcctgttc ctattgcaac agccttttcc gcgatccaat cggaagcaga aaccgctatt
     1921 ggcatatcct ttaatggcag acccatattc tttgcgagtt caatggccag aagtatcact
     1981 cttgagttat caacgcagct cccaaaatgc cataccggag gaagttgctt acctgagaac
     2041 tcacccagag cctttaatac tcctttcaat ttatctcctg catattccat cgtggcttca
     2101 ggagtgagaa gtccccctct tgcacatgat tgcgcggaac atcccgttga gaaaacaagt
     2161 acattttttt ttaataattc ttttgcgatg atcacctggc tggactcgat agtaccaggg
     2221 cttatgcagc ccgccatgag gacaattcca tatatatctc ctgatgttat gctgtcaatt
     2281 aatggtttga acgggtcttg cggattcatt ttagcgagga catcaagaac gttctcaacc
     2341 gagaaacctg ccataaggtc atgagtcttt tcttgcggca gaaaaatatt ttttcctctg
     2401 ttcgggaaat tctcaatagc gcaatccagg atcctttttg cagtgtcatc ggcattttct
     2461 tcatgaaaag gtatatgttg cgcaccctgg aatctagctt ctttcatggt tgagatgatc
     2521 ctggtatgaa aataccgtgc aacattctca atattgggat aaatgcactg gacatctacg
     2581 actatggctt cagcaagtcc cgtggctatt atcagttcct ggtgaatatt gcttcccgca
     2641 atagggatgc catgcctcat taacgcatcg ttcccggtgc agcagcagcc tacaacattt
     2701 attccattag cgccaagcgc tcttgctttt tccaggagct gcgcatcctc ggaaaattca
     2761 atgatcttct cggaaaggag aggaacatga ccatgaatta ctatgttcac cttatctttt
     2821 tccagcacat tgaacccgat ctttgaggag aatagttttg ggtttccgaa aagtacatcc
     2881 tgtaattccg agctgattat aagtgatgaa atatcagcaa ctccaagcct tgccgcatgg
     2941 agtacgaact cttttggatc agccatggta cccatagaag tttcatgccc cgaatccagc
     3001 aactcccgac ctgcggatgc aggcattact ccaagatttg aaagattccc gacaatatcg
     3061 gcaggataat atgactcggc aaatctcatg tggtcttcat cccccagtat atcggctatc
     3121 gccgctgaag cagtagcaca ggccatatct tcaattccat tttctttgcc aatattgatc
     3181 ttacgggcaa ttgattccaa ttttatggat tcttttatct tataaggtgt ttttttcctg
     3241 gctgtcttaa gcagcgttgt tgccacatgc agggcatggg aagcatgtgc ggctgtccct
     3301 ctgccagtca tcataagaag atttcttgcc gcaatagtat cagcgcttgc gccacatatt
     3361 ccttttagag aacgaccgga aatcctgcag ggccccagag cgcattgctt acagcatatc
     3421 cctcttgagc caaaattaca tttggctttc ttgcattctc cagtattgta atttccattc
     3481 ctatcgaaaa tggtttctat tccaagcaca tgtgcgtgct tgatcatttc attagttgca
     3541 tcatccattt tttgggtctc ttctttcata gagcctccat gatcatgcct cttcactttt
     3601 ttttcgcctt caatggtaaa atagcactct tgtttatata tttcaaacca tattgaaatc
     3661 cgcaactagg ttgaaatgtt ttaagggtat tgcaatttat aaaattattt aaaaattaag
     3721 atatgttgcc caggagtggc atttgtgggt gttattgatt tattgcgttt tcaggaaaca
     3781 tgagccagaa ccacaaaggc aaataagtaa ttttcttatc tcctatgatt tcctctcctt
     3841 catagtcctc ggttattata agaccgcttt ccagatcaaa ttcattcaaa gcgcaaagca
     3901 atccctcaac ttcccgctcc ttgacttccg attgactgat atcccagcat acctgaatag
     3961 cttcgcttat cttgaattcg ttcataagaa gaaaatccgt ttccctttga tttttatcgt
     4021 gccagtagaa tattctagac aggggattag aaaagtttct ccttttcagc tctatgaaga
     4081 caatattttc tgcaattcgt ccgtaatcca gagaaaatct gaatcctttt atattcctta
     4141 tcccactatc gatgcaatat attttcttag gatttatttc ctgcgctttg acagaaaagc
     4201 taaatatcgg cacattaaaa acaagatatg catcctttag atttgccgca tactctccta
     4261 ctgtatccac agagagagaa atattttttc caactttact gaaattaata gtgtttgata
     4321 tgttttgcag tagtaaattg accattttct ccatttttac tgcatttctg atgcctcgtt
     4381 tttttattat gtctctgtaa attattgtat caaaataaaa ctgaagtagc tgttttttta
     4441 tgtccgggtt attctcaagg acaacttccg ggaaacctcc aagttccata tattcaaaga
     4501 ggtgatgttt tatttcgtct tttttggaaa aagcagaaat tttatctttg atcgtgagcc
     4561 ttgttttgag aaattctctg aaacttaagg gataaacttc gaagtaagct atcctgccag
     4621 caaggctata actaaaatcc tcttttaaca ttgagaagtt tgaacctgaa ataatgagtt
     4681 tcatgtcttt ttccagatca tatatccttt tcagtgtatt ctcccactta tctacctcct
     4741 gtacttcatc aatcaggaca aaaattttgc cttcaggatt tatcagttct ttgtagcttt
     4801 ttattgcatc atcgatcaag ttctcctttt gaaccctatc gtcatcgcat tttacaaaaa
     4861 gaatgttttt ttgcgggatt gtcgaactta atcggcttat tagaatcttc attatggtgg
     4921 tcttgccgga tctcctgacc ccaataaatc ctaaaatttc ttttctgtca agccattgtt
     4981 cgatatcttt taatagttcg cgttcaacca gctttaattc tttctcctcc ttccagtggg
     5041 gattccattc aaccaggatc tctttgttca ttaattccat tatagtcgaa tcaagtattt
     5101 aaatcactcg attataatct agtaattttc tttcggaaaa gatgaagaac aaaagatatg
     5161 agaatgacct tataagaata agtgttttat tgaagcaaat atgagcctca tattaatagc
     5221 aataggtggg aacgcggtat taaatccgga aaagggaaac ccggaagaac aacaggaaaa
     5281 gatagatgaa acaagcaggg agatcacccg gataatcagg gaaggtaatg atgtggtttt
     5341 aacgcacggc aacgggcccc agataggaaa tatccttgcg atgcaggagg aatgcggact
     5401 cgtgcatccc cagccgctgg atatatgcgg ggcccagacc cagggcatgc ttggttattt
     5461 gctgcagagg tcactttcga acatgttccg ggaaatcggg ataaagaaac aggtggcaac
     5521 aattataacc caggtgatag ttgatggatc atgcgattca ttccacaacc ccacaaagcc
     5581 gataggcctt tgttatccgg aaggaagagc gaagacgatg atgaatgcgg gcgtgaagat
     5641 gatccatgat agaaaaggat accgcagggt agttccctct ccaatgccca tcaaaattgt
     5701 ggaagaagac gtcattaaga agcttatttc tgaaggtgta ctggttatca cagcgggtgg
     5761 gggagatatc cctgttatgg agagaaacgg cgtactcgaa g
//
LOCUS       DATGBN010000480         4802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_37794, whole genome shotgun sequence.
ACCESSION   DATGBN010000480 DATGBN010000000
VERSION     DATGBN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37794"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..476
                     /locus_tag="VKL21_12280"
     CDS             135..476
                     /locus_tag="VKL21_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46587.1"
                     /translation="MDKQIAYGTGLLAWLMQRITGLLLVIYLFIHVWWVHFPGVKTPF
                     DFLINTLFLPSRMAILVLLVLVIPHTLNGFRVFVIDFGISERAQRILFWALTGFGIII
                     IAWSYYIRFYT"
     gene            573..1205
                     /locus_tag="VKL21_12285"
     CDS             573..1205
                     /locus_tag="VKL21_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HMB46588.1"
                     /translation="MSELENNIKNAAVKYDSARYNFFKWRFESPVLNKVVLAFAFACF
                     TGILAQLRFYLPYTPVPVTGQVFAVLLSGVMLGKWYGGMSQGLYAGLGAAGIPWFSEG
                     KAGMEILSGVTGGYIIGFIFASLIIGWFTDRYLKFRSFAGLFSLMVLGIAIIYLFGVI
                     QLSVVLGVNVQKAIELGALPFVVVDIYKALIVSAIAAAIIPKKPFGNELG"
     gene            1233..1694
                     /locus_tag="VKL21_12290"
     CDS             1233..1694
                     /locus_tag="VKL21_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOB1 family endonuclease"
                     /protein_id="HMB46589.1"
                     /translation="MIYIADSSLFIIGKRIEGIIITVPSVLDEIRDEKSRTIMELMNV
                     RIEPVLQSFIREVISKARETKDSEELSRTDLDLLAKALEHTRNENTILVTDDYAVQNT
                     AIQLGIKVMPTGQKKIKDVLLWEKFCIGCKRRFPTGDDCPVCGTHLKKMRK"
     gene            1947..2324
                     /locus_tag="VKL21_12295"
     CDS             1947..2324
                     /locus_tag="VKL21_12295"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMB46590.1"
                     /translation="MNNKIQTIGMMEIILKEINEAGGIEASAIASRDGLLICTTLSDQ
                     HQAEIFVAMSATMIGAAETAAAELGKGIPDITIVESQSGKIIGTGAGPKALLLVMTRP
                     DAGLGLVLIEMKKASEKIKSILG"
     gene            complement(2328..3620)
                     /gene="rbcL"
                     /locus_tag="VKL21_12300"
     CDS             complement(2328..3620)
                     /gene="rbcL"
                     /locus_tag="VKL21_12300"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HMB46591.1"
                     /translation="MTGKIDWYNEFVDLNYKPAKDDLVCLYYFEPAKGITAREAIGRI
                     ASESSAGTWTTLHDVPARVEKIKARAFELNGKYVKIAYPADLWEPGNAPQLLSGIAGN
                     IFGMKALRNLRLLDASFPLRYIKSFKGPNHGIGGIRSLLKIKERPITGAVPKPKIGFS
                     AAQHADVAYETWMGGFDLVKDDENLTSTSFNRFEDRLSRMTKLRDKAEKETGEQKDAL
                     LNITGETREMIRKAKLLHDAGWRFAMIDVVTCGTASVQTMRDECGDLGLAIHAHRAMH
                     AAFDRNPRHGLSMYFLAKLMRLIGVDEIHSGTAIGKLVGSKKEVIQVAKVLRDPGIPA
                     GDMLSQDWGSIKPVLPVSSGGLHPGLIPSVMGILGNDCTLLVSGGIHGHPKGTRAGAK
                     AVMQAIEATMGGMGLDEFARKNKELDQALGKWEYYRPK"
     gene            3807..>4802
                     /locus_tag="VKL21_12305"
     CDS             3807..>4802
                     /locus_tag="VKL21_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HMB46592.1"
                     /translation="MVSNDFLHSGRALRIILIITVLGLLLVGGAGAQNSYEFVQKIPT
                     SVQWNFYHPEGVAVDSAGNVYVSDSENNRIRKLSSSGVFLVSWGSRGTGDGQFADPEG
                     IAVDSAGNVYVSDSGNNSRIQKFSSAGVFLAKWGGPGIGDGQFDDPQGVAVDSAGNVY
                     VSDSGNNSRIQKFSSSGVFLSRWGSPGSNDSQFKDPEGVAVDSAGNVYVSDSKNNRIQ
                     KFSSSGVFLTKWGGRGIGDGQFKNPGGVVLDSFGNVYVSDSRNNRIQKFSSSGVFLAK
                     WGGPGIGDGQFQDPKGVVVDSAGNVYVSDRLNDRIQKLSSEGVFLTKWGGSGIGDG"
BASE COUNT         1297 a          963 c         1115 g         1427 t
ORIGIN      
        1 aaaggcgtct gggcttgtga ccatgtttat aggtgtgttg aggtctgtcc taaacaggtg
       61 ccgcctacaa atgccattac aggacttaca agaaaaataa tattgcagaa aatgaaaaac
      121 atattgaggt gaccatggat aaacaaatag cttacggcac aggcctgctg gcatggctga
      181 tgcagcgtat tacgggatta ttgctggtaa tttatctatt catccatgtc tggtgggtgc
      241 attttccggg agttaagacg ccatttgatt ttttgataaa tacgcttttt ttgccgtctc
      301 gtatggctat cctggtttta ttagtgcttg taatccctca tactcttaat ggttttcgag
      361 tttttgtgat agattttggg atatcagaaa gagcacagag aatattattc tgggcgctta
      421 cgggtttcgg gatcattata atagcctggt cttattatat cagattttac acctgaaggg
      481 tcgtattata ccagatttta cacattatat tcccagtaac ctttattatg attgtctacc
      541 tttttgaaaa aaaagtttac aataggtgtt atatgtccga actggaaaac aatataaaaa
      601 atgcagcggt aaaatacgac tcggccagat ataatttttt caaatggcgg tttgaatccc
      661 ctgttttgaa taaagtcgtt cttgcttttg cttttgcctg ctttacaggc attcttgcac
      721 aattgaggtt ctaccttccc tatacgcccg ttcctgtcac aggccaggtt tttgcggtcc
      781 ttctctcagg cgtgatgctt ggaaaatggt atggcggcat gagccaggga ttatatgcag
      841 gattgggcgc tgccgggatt ccctggttca gtgaaggaaa agcaggaatg gaaatccttt
      901 ctggcgttac tggtggttat atcattggat tcatctttgc atcgttaatt ataggctggt
      961 tcacagaccg gtatctaaaa ttcaggagtt ttgcaggcct tttcagtctg atggttcttg
     1021 gtattgccat aatctacctt tttggcgtga tccagttatc agtggtactt ggagtgaatg
     1081 tgcaaaaagc cattgaattg ggcgcattgc cattcgttgt tgttgatatt tataaagcgt
     1141 tgatcgtttc ggcaatcgct gctgcaatta tccctaaaaa accattcggg aatgagctcg
     1201 ggtaggatca acttttttta aataccataa gaatgatata catagcagac tcctcccttt
     1261 ttataatcgg gaaaaggatc gaaggaatta tcataaccgt accctcagtt ctcgatgaga
     1321 tcagggatga gaaatcacgc acgataatgg aacttatgaa tgtgaggatc gagcctgtgc
     1381 ttcaaagttt catcagagaa gtaatatcca aagcaagaga aacaaaagat agtgaagaac
     1441 tttcaagaac ggacctggac ctgctggcaa aagctcttga acatacaaga aatgaaaata
     1501 ccattttggt taccgatgat tatgctgtcc agaacactgc aatacagctg ggaataaagg
     1561 tcatgcctac aggacagaag aaaatcaaag atgtacttct atgggaaaaa ttctgtatcg
     1621 gatgcaaacg aaggttcccg accggagatg actgccctgt ctgtggtacg catcttaaga
     1681 aaatgaggaa gtgattattt ttaaccggaa aagattgttc aaaccttcac acttttattt
     1741 atataaataa ttaggaatat tgcttttttc tccattgatt ttgggttgga atctttctca
     1801 gggcatctat tgaattattt gcgtacaatc agtttatttc tatccatgct tccttcatga
     1861 gcaaatgttg ctcttttgca cagttttaaa tagtaagaag cgcatattgt cagttgtcga
     1921 aaaattgaac cgaggaaggc gttattatga ataataaaat ccaaacaata ggaatgatgg
     1981 aaataatatt gaaagagatt aatgaagccg gtggaatcga agcatcggca atagcaagca
     2041 gggacggttt attaatatgc acaacattat ccgaccagca tcaggcagaa atatttgtgg
     2101 ctatgtccgc aactatgata ggcgcggcag aaacagcagc cgcagagctt ggaaaaggca
     2161 ttcccgatat aaccattgtg gaatcacaaa gtggaaaaat cattggtacc ggagctgggc
     2221 caaaagcatt gttattggtt atgaccagac cggatgcagg tcttggatta gttcttatcg
     2281 aaatgaaaaa agcatccgaa aaaataaaga gtatccttgg ataactttca ttttggccgg
     2341 taatattccc atttacctag cgcctggtcc aattccttgt ttttcctggc aaattcatca
     2401 agtcccatac cacccattgt ggcttctatt gcctgcatca ctgcttttgc acccgctctt
     2461 gttcctttcg ggtggccgtg aatgccgcca ctcacaagca gcgtacagtc atttcccagt
     2521 atgcccatta ccgatggaat cagtcccgga tgaagaccac ccgaagagac cggaagtacc
     2581 ggttttattg agccccagtc ctgcgaaagc atatctccgg ccggaatccc ggggtcccgc
     2641 agtactttag ctacctgtat gacctctttt ttacttccca caagttttcc aatagctgta
     2701 ccggagtgaa tttcatcaac tccaattaac cgcataagtt tggcaaggaa atacatggac
     2761 aaaccatgtc ttggattcct gtcaaaagcc gcatgcatcg cacgatgagc atgtatggct
     2821 aatccaaggt cgccacactc atcccgcatt gtctgtactg aagctgttcc gcaagtgaca
     2881 acatcgatca ttgcaaatct ccaacccgca tcatgcagca attttgcctt acggatcatt
     2941 tctcttgttt cgccagtgat atttaaaagg gcgtccttct gttcacccgt ttccttttcg
     3001 gccttatccc gcagttttgt catcctggaa agcctgtctt caaaacggtt aaaagacgtg
     3061 gaggtcaggt tctcatcatc ctttacaagg tcaaatccac ccatccaggt ttcgtatgca
     3121 acatcggcgt gttgggctgc actgaatcct atcttgggtt ttggcaccgc gcccgtgatg
     3181 gggcgttctt ttattttcaa taaagatctt attcccccga taccatggtt tggccctttg
     3241 aaagatttaa tgtatcgtaa ggggaaggat gcatccagaa gcctgaggtt tcggagagct
     3301 ttcataccga agatatttcc ggctatcccg ctcaggagct gaggggcatt tcccggttcc
     3361 cagagatcgg caggataagc gatctttaca tatttaccat ttaattcaaa agcgcgcgct
     3421 ttgatctttt caacccttgc cggcacatca tgaagtgtag tccaggtacc tgcagagctc
     3481 tcggaagcta tccttcctat cgcttccctt gcggtaattc cttttgcagg ttcaaaataa
     3541 tataaacaga ccagatcatc ttttgcgggt ttataattca aatccacgaa ttcattgtac
     3601 caatcaattt tcccggtcat ttaatcacca ttctccttct attatcttag aaattgaaag
     3661 atttaactgt aatgtcacat aaagagtttc cacaggcaaa atgttaaaag gaaaagtctt
     3721 aaatacattc cgcaactact atttttcata gtagatggcc ttgattctgt ggagcgggct
     3781 gtatatttat gaagaggtga taataaatgg tttctaatga ttttttgcac agcgggcgag
     3841 cactaaggat tattttaata ataacggtat tgggattatt gttggtcgga ggggcaggag
     3901 cccagaattc atatgaattt gtccaaaaga tccctacatc agtccaatgg aatttctatc
     3961 atccggaggg agtggcagtt gatagcgcag gcaatgttta tgtttccgat tcggaaaata
     4021 accgcatccg gaagttgagt tcatcaggcg tctttttagt ttcgtgggga agccggggca
     4081 ctggcgatgg ccagtttgcg gatccggaag gaatagctgt ggatagtgct ggtaatgtct
     4141 atgtttctga ttcgggaaat aatagccgca tccagaagtt cagttccgcc ggcgtctttt
     4201 tagctaagtg gggagggccg ggtattggcg atggtcagtt cgatgatcca cagggagtag
     4261 ctgtggatag cgctggtaat gtttatgttt ctgattcggg aaataatagt cgcatccaga
     4321 agttcagttc atcaggcgtc ttcttatcca ggtggggaag ccccggcagc aatgatagcc
     4381 agttcaagga tccggaggga gtagctgtgg atagcgctgg taatgtctat gtttctgatt
     4441 caaaaaataa ccgtattcag aagttcagtt catcaggtgt ctttttaact aagtggggag
     4501 gccggggcat tggtgatggc cagttcaaga atccaggggg agtagtttta gatagttttg
     4561 gtaatgtcta tgtttctgat tcaagaaata accgcatcca gaagttcagt tcatcgggcg
     4621 tttttttggc taagtgggga gggccgggca ttggcgatgg ccagttccag gacccgaagg
     4681 gagtagttgt ggatagcgct ggtaatgtgt atgtctctga ccgcctgaat gaccgcatcc
     4741 agaagttgag ttccgaagga gtctttttaa caaagtgggg aggctcgggt attggcgatg
     4801 gc
//
LOCUS       DATGBN010000481         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_154781, whole genome shotgun sequence.
ACCESSION   DATGBN010000481 DATGBN010000000
VERSION     DATGBN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154781"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VKL21_12310"
     CDS             <1..186
                     /locus_tag="VKL21_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46593.1"
                     /translation="IYSSQSFQPEPSESHHPSQPRSLFPRTPMLNKIITQLETERKKA
                     DGEANEKIERLKKLGKE"
     gene            complement(429..1394)
                     /gene="ftsZ"
                     /locus_tag="VKL21_12315"
     CDS             complement(429..1394)
                     /gene="ftsZ"
                     /locus_tag="VKL21_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMB46594.1"
                     /translation="MNEGFTKIIKANRPSVAVIGLGGAGSNIITFLSEKNIAGAKIIA
                     ANSEISHLVLQKADKLLLLGKETSKGKGCGGFPDIGAECARESAEEIRKELEGANIVF
                     IVAGLGGGTGTGCAPVVAEISHSMGALTIACLTMPFEIEAMRRENAKKAIRALSETCD
                     SVVLIDNTKLRQMAGNLPLKTAFGVANSLIGLFIKSITETITIPSLMNLDFADLKAVL
                     EKRGVSSFGIGESEGPDRVKKAVLKAVSAPLLDIPDLGSAHGLLIHINGGRDLTLEEV
                     AYVGELMAEQLPGTKRIIWGANVDETMTGRIRIMALFASVGNPFE"
     gene            1525..1824
                     /locus_tag="VKL21_12320"
     CDS             1525..1824
                     /locus_tag="VKL21_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46595.1"
                     /translation="MEQNKNVNINYNNSVLPEYSPVVDIAKKDLSAILNIPVSDISLI
                     KEEAVEWPDASLGYPEKGKMYAQVITPGFKIILKADNKLYEYHSDYKRVAGPIEI"
     gene            complement(1826..2455)
                     /locus_tag="VKL21_12325"
     CDS             complement(1826..2455)
                     /locus_tag="VKL21_12325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMB46596.1"
                     /translation="MVRRKTNPLQNSLNNFIAAIKSSYSLTILILAIVSFLLQYIIPG
                     YSYYLALSPYDIFSRPWTLVTHIFLHSGPIHLLFNMMFLFFLGPELERRIGGKKFLIV
                     FFISGIIAAIGYSLWSVFILKEHSTAVGASGALFGIFACLAVIAPDIVIYVYFIPMKI
                     TYALIFFALLDLLFIGSSGDLVARSAHLSGVVAGLAFGKYLKKTGRYLR"
     gene            complement(2522..2629)
                     /locus_tag="VKL21_12330"
                     /pseudo
     CDS             complement(2522..2629)
                     /locus_tag="VKL21_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013587.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger protein"
     gene            complement(2666..3376)
                     /locus_tag="VKL21_12335"
     CDS             complement(2666..3376)
                     /locus_tag="VKL21_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMB46597.1"
                     /translation="MTQLILKAVDIEKEIKCIGSFPDEDYIKIISEVGFVCDFCGKCC
                     TSEFNDHVFLLDDDAQKIIEHNGRKYLRHAPYFDLCDNLGIFYVMGYALKTKPNGDCI
                     FLRDGRCENYEIRPRICKIYPYMLHREPDDEGNFEFRQISGLDMHGLYHNEISEESCK
                     EILKSVKEYESGFQRQKLGFIKEIERFFKENNLRNSQQMYDRMMREYHKGKVIEVNIF
                     FRGKFEKEIISKQINNPE"
BASE COUNT         1161 a          718 c          646 g         1130 t
ORIGIN      
        1 atttattctt cgcagtcctt ccaaccagaa cccagcgagt ctcatcaccc cagtcaaccc
       61 cgatcacttt ttcctcgaac acccatgctt aataagataa taactcaact ggaaaccgaa
      121 cggaaaaaag ctgatggtga ggctaatgaa aaaattgagc ggttaaagaa attggggaaa
      181 gaatgaatta ttcatcttca gtaaattgga caaaagatac acggaatatg tggcttagca
      241 tagtgaataa ttattattac tctttaaatt ttaatgtaac taaaaagagt ccgtgaagat
      301 ccgcgcaatc cgtgtcatcc gtgttccatt aaaactaaac ctgataaaca ttgaaaatta
      361 ataactcttt gaataactgt acagagcaag tattttaatt ccttaaccga taacaaatgg
      421 tgtagaattc attcaaatgg gttgccaaca cttgcgaata atgccataat ccggatgcgt
      481 ccggtcatgg tctcatcgac gttggctccc cagattattc tttttgtacc agggagttgc
      541 tctgccatta attcacctac gtacgccact tcttcaaggg tcaaatcccg tccgccatta
      601 atatggatca ataatccatg cgcagaccca agatcaggaa tatccagaag gggggcggac
      661 actgctttta atactgcttt ttttacacgg tcaggacctt ccgattcccc gattccgaat
      721 gaggaaacac ctcttttttc aagcacagct tttaaatcgg caaaatcaag gttcataaga
      781 cttggtatgg tgatggtttc cgtaatactc ttaatgaata atccaattag agaattcgca
      841 acaccaaaag ccgtcttaag aggcagattt cctgccatct gcctgagttt tgtgttatct
      901 atcaaaacca ccgaatcaca tgtttcagaa agagcacgta tggccttttt ggcgttttca
      961 cgcctcatgg cctctatttc aaaaggcatt gtcaggcatg ctattgtgag tgcgcccatg
     1021 gaatgtgaaa tttcagcaac aacaggggca caacctgtgc cagtcccgcc tcccagtccc
     1081 gcaacgataa atacaatatt tgcgccttca agttcttttc ggatctcttc tgcgctttct
     1141 cgcgcgcatt ctgctccaat gtccgggaat cctccgcatc ctttaccctt gcttgtttct
     1201 tttcccagca gcaataattt gtccgctttc tggaggacaa gatggctgat ctcagaattt
     1261 gccgcaataa tttttgcgcc ggcgatgttt ttttcagaaa ggaaggttat gatgttgctt
     1321 ccggcgcccc caagaccgat aacagctaca gatggtctgt ttgctttgat tattttcgta
     1381 aatccttcat tcattatagg ggtttaatat agtctaattg atataaatat cttacataca
     1441 tattgacata tgcccacgaa ataatatgat attatgaaaa gattaatcat cctatttata
     1501 ttatcaattt atatatcagg atgcgtggaa caaaataaaa atgtgaatat caattataat
     1561 aattctgtcc ttcctgaata ttcacctgtt gtggatattg cgaagaagga cctttccgca
     1621 atattgaata tccccgtttc agatatttca ttgataaaag aggaagccgt agagtggccg
     1681 gatgcaagcc ttggatatcc tgaaaaaggt aagatgtatg cacaggtaat tactccgggg
     1741 ttcaagataa tattaaaagc agacaataaa ttatacgaat atcatagtga ttataaaaga
     1801 gtggcagggc cgatagaaat atagattaac gcagataacg tcctgtcttt tttaaatatt
     1861 ttccaaaagc cagacctgct acaactccgc ttaagtgtgc gctccgggct accaggtcgc
     1921 cgcttgagcc aatgaacaaa agatcaagga gagcgaaaaa tatcaatgca taggttattt
     1981 tcataggaat aaaataaaca taaataacga tatcaggcgc aataacagca agacatgcaa
     2041 atatcccgaa cagggcgccg cttgctccaa cagctgttga atgttctttc aaaatgaaaa
     2101 ccgaccataa agaataacct attgcagcga taattccgga aatgaaaaat acaatgagga
     2161 atttttttcc tcctatccgt ctctcaagct caggtccgag aaagaatagg aacatcatat
     2221 tgaataaaag atggatcggt cctgaatgaa ggaaaatatg tgtgacaagg gtccatggcc
     2281 gggaaaaaat atcataggga gaaagagcaa gataatatga atatcctgga ataatatatt
     2341 gcagtaaaaa agatactata gctaggatga gtatagttaa tgaatagctg ctttttattg
     2401 cagctatgaa attatttagc gaattctgga gagggtttgt ttttcttctt accacggtat
     2461 ccctcttata aaagattccg atatcgcttg atttcgattt atattccaaa agtccgggac
     2521 aattatgtga ttcgggaagt cggtggttat aacagtatga attgccgcag aatttgcatg
     2581 tgaacggcat ggggtccctg aaaccgcaat aataacattc tttggtcata aatatcaatt
     2641 ttttcaaagt taaataatct taatatcatt cagggttatt aatttgtttg gatataatct
     2701 ctttttcaaa tttgccccgg aagaatatat tgacttctat tacttttccc ttatggtact
     2761 ctctcatcat tctgtcatac atctgctggc tgttccttaa attattttct ttgaaaaatc
     2821 tctcgatctc cttgataaat ccaagttttt gcctctgaaa tcccgattcg tattctttta
     2881 ctgatttcaa tatttctttg cacgattcct cacttatttc attatgatac aatccatgca
     2941 tatccaagcc actgatttgt ctgaattcaa aatttccctc gtcatcaggt tccctgtgaa
     3001 gcatatacgg atatatttta catatccggg gacgaatttc ataattttca catcttccat
     3061 ccctgaggaa aatgcagtct ccgtttggtt ttgttttaag ggcataaccc attacataaa
     3121 atattccaag gttgtcacaa agatcaaaat aaggcgcatg ccttaaatat ttccttccat
     3181 tatgctcgat tatcttttga gcatcatcat cgagaagaaa gacatgatcg ttgaattcac
     3241 tcgtacagca cttaccgcag aaatcacaaa caaatcccac ttcactgata atttttatat
     3301 aatcctcatc aggaaatgaa ccaatacatt tgatttcttt ttcaatatca accgctttca
     3361 atattaattg agtcaacttt ttctgataag tagtgtcatc tatttatatt attctttcac
     3421 tatgtcactt gaaagaacat aataaataat gataaaaaaa atgataagtt atataaagca
     3481 tcaagttaag agtgatttgg caaaaacgga aaaacggggt cctaagtaaa ggatagtaca
     3541 aatatgagag gtatcattga atgtctctaa gcaaaaaaaa atattcacta tttaaaaata
     3601 aatatgtatt gattttaacg attttgttat cgttatttat cggtgtttct actgc
//
LOCUS       DATGBN010000482         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_388164, whole genome shotgun sequence.
ACCESSION   DATGBN010000482 DATGBN010000000
VERSION     DATGBN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388164"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2491)
                     /locus_tag="VKL21_12340"
     CDS             complement(<1..2491)
                     /locus_tag="VKL21_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46598.1"
                     /translation="MRRVKEKIYDHLYACQQQCGGYTGKDIEEIARHLDCNRKTIKRD
                     LEKWTAADPLFAELKYIGKRSIPITLEDIAILNQLLSENITIRKQELIREINEKRIKR
                     GETSISQRPLYHVINNLIQTLTNGSPQEHHWLVTQGIEVSDMYNLADARTMLSNTFIY
                     SGLKIFGGIDIDGIALRLQEAQKQFQESYPSADPFNWFFRIRPRSKVIRNHLSKINAD
                     ESLSNQARLIFEAQVDFIVRINDILIDELIHKEGWIQYSADANRQKIENSIRTEWINK
                     YYETANKVIINPNEENFSTLKILMDQGMPESEEAELELLGKHRMKYEHIYRHLEELTN
                     NFSEDKIIPHHATAHLLLDLCNGRRKWASLNEEEKKKMITNSIIQNQEQFLKSILTKK
                     LVRYIKKGKITLERSFKYQDISKIIESVDLSDTDRIVTKEDIEKLIKGTYPINLETNI
                     SDIAHVSSPDEGVDSEYQPYNKIPFQQVQNQVLGIVANHNPAWFQDHQQIFDRMTDSM
                     FEMEYDELTFRTKLYEAIGFLGRNLRYSDSPSFQSLRYFIQRYLSDATLDLEFRHLWK
                     LYEDLTGHKTPVIIIDSMGIDSRKKSLFAKIHGRYYTIGFIDVRAVSPYLIPMFSSNY
                     RSTDSEAMNIIEIVNQAKSVLGEEFKFCTGNGHTVSRIAAGLAFADHKVILLGRNPNP
                     PKKPGKICLFRLNKHLDLINKVGKLVREDSEFGRIIATREHIYVNGVNVRNLLRDLGR
                     LILWNSEQKGYDLDALIQLVETSNRQKRLVRIVERGVTRVNEPNVSLSMKSAELILLM
                     SAIANVLNGSGSLKWEGWSPIKMENIALFIPA"
BASE COUNT          904 a          588 c          545 g         1119 t
ORIGIN      
        1 aagcaggtat aaacaaagca atattttcca tttttattgg actccatcct tcccatttta
       61 atgaccctga gccatttaat acatttgcaa tagcggacat taacaatatc aactctgctg
      121 atttcatcga caaactcaca tttggctcgt ttactcttgt aacgcctctt tcaacaattc
      181 tgaccaatct tttttgtcta tttgaagttt caaccagttg aatcaaggca tccagatcat
      241 aacccttttg ttctgagttc catagaatta gtcttcccag atctcgaagc aggtttcgta
      301 cattgacacc attaacataa atatgctctc tggtagcaat aatcctgcca aattcactat
      361 cttcacgaac caatttaccc actttattta tcaaatcgag gtgcttgttc aaacggaaaa
      421 gacaaatctt gcctggtttt tttggtggat ttggattcct gcccaatagt atcactttat
      481 ggtctgcaaa ggcaagacct gctgctattc ggctaactgt gtgtccattt cccgtgcaga
      541 atttaaattc ttctcccagt acacttttag cctgattcac gatttcaata atattcattg
      601 cttcactatc tgtagaacga taattgctgg aaaacatcgg aatgagataa ggagaaacgg
      661 ctctcacatc aataaaacca attgtgtaat atcgcccatg aatctttgca aatagactct
      721 tcttgcgact gtctattccc atcgaatcaa tgatgataac aggagttttg tggccagtca
      781 ggtcttcgta tagcttccat agatgtctga attcaagatc taaagtggca tcgctcagat
      841 aacgctggat gaaataccga agactttgaa aagaagggga atctgaatat cttaaatttc
      901 gtccaagaaa accaatagct tcgtacaatt ttgtccggaa agtcaattca tcatactcca
      961 tctcaaacat actgtccgtc attctgtcaa aaatttgttg gtgatcctgg aaccatgcag
     1021 gattgtggtt agcgactatc cccaacactt gattctgaac ctgctgaaag ggaattttat
     1081 tatatggttg atattcagaa tcaacacctt catcaggact tgaaacgtga gcaatatctg
     1141 agatatttgt ttcaagattt attgggtatg tacctttaat caatttttca atgtcctctt
     1201 ttgttactat ccggtcggta tcagagaggt caactgattc tattattttg ctgatatcct
     1261 gatatttgaa tgaacgttcg agtgttattt tccccttttt tatgtatcga accagcttct
     1321 ttgtgaggat agatttcaaa aattgttctt gattctgaat aatcgaattg gtgatcattt
     1381 ttttcttttc ttcttcgttg agggatgccc atttacgcct tccgttacat aagtccaata
     1441 gaaggtgtgc tgtagcatga tgcgggatga ttttatcctc tgagaaatta tttgtaagtt
     1501 cttcgagatg tctgtatatg tgctcgtatt tcattcggtg ttttcccaga agttctaatt
     1561 ctgcttcttc cgattcgggc ataccttgat ccatgagaat ttttagtgtg gaaaaattct
     1621 cctcatttgg attaattatc accttatttg ctgtttcata atacttgtta atccattctg
     1681 ttcgtatgct gttttcgatt ttctgcctat ttgcatctgc agaatattgt atccatccct
     1741 ctttgtggat aagttcgtct atcaggatat cgttgatacg cacgatgaaa tcaacctggg
     1801 cttcaaatat gagacgtgct tgattggata gggattcatc ggcattgatt ttgctcaagt
     1861 gattccttat cacttttgat cttggtcgaa tacgaaaaaa ccaattaaaa ggatctgcac
     1921 ttggatagct ctcctgaaac tgtttttgtg cttcttgtaa ccttaatgca atgccatcga
     1981 tatcaatacc gccaaagatt tttaatcctg agtatatgaa agtgtttgat aacatggtac
     2041 gggcgtctgc taaattgtac atgtcagaaa cttcaattcc ttgtgtaacc agccaatggt
     2101 gttcttgggg tgaaccgttg gtcaaagtct gtatcaggtt attaatgacg tgatatagtg
     2161 gtctttggga aattgaggtc tctcctcttt ttattcgttt ttcgttaatc tctctaataa
     2221 gttcttgttt tctgatggtt atgttttcag aaagaagctg atttaatatc gctatgtctt
     2281 caagtgtaat gggaattgaa cgcttaccga tgtatttcag ttctgcaaaa agaggatctg
     2341 ctgcagtcca tttctccaaa tctcgcttta ttgtttttct attgcaatcc aaatgtcttg
     2401 ctatctcttc tatatctttt ccagtgtaac caccacactg ttgctgacat gcataaagat
     2461 ggtcgtatat tttttcttta actcgcctca tcggttaacc tcactggtta attcgatagg
     2521 aacatattaa attttattta aaattggtat cctcttcata aaccatggta actctctgtg
     2581 ttatgaagag ttattgttcg caaaccgtat tctgtagaca tatgtggcat tcataatgtt
     2641 ttgtttatgc aaggtttacc attacttttg aagaggatac taaaattgtc cgattcaaac
     2701 aatttacccg ccatgaaggt atagactgta taacctatct tcttctatat gtcgtataaa
     2761 tcatctcata aatgcaatat gagcgattat caaggggtgg atataggtta agtcatcctt
     2821 atatgttgaa taataccgtt tcaattcgtg ttggcaatta tcctaaagtg caacgcttct
     2881 acttatgttg ggaagaactt atcacaaaaa atgaatctaa ccaaaaatta gatatttgct
     2941 aatttgctat ttctccctag aaaaaaatat attcaacttg ctttattgtt tcattatgga
     3001 aaaagaggga aatgaaggta taatatttaa atatcggaaa attaatgaca atacaaaaaa
     3061 taatctaata aataatgaat tatttttcag tcaccctgaa gatttcaatg atccatttga
     3121 tagtaaaatc gaagtagaat ataaaggtac aataaa
//
LOCUS       DATGBN010000483         5005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_76839, whole genome shotgun sequence.
ACCESSION   DATGBN010000483 DATGBN010000000
VERSION     DATGBN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76839"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VKL21_12345"
     CDS             <1..218
                     /locus_tag="VKL21_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46599.1"
                     /translation="LKELGEIAETLIKKDRPEHWKNELGDLCGLCIKPMLELAETDFD
                     TACQVGINRRQQKMHENHIKNNGASAP"
     gene            complement(325..1521)
                     /locus_tag="VKL21_12350"
     CDS             complement(325..1521)
                     /locus_tag="VKL21_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMB46600.1"
                     /translation="MAYNENTDDIHGFDLKLSQSMAKFNKVKTSDRNKQLVADFLKTH
                     KRKGNRKSTLAANCAIFIQIISRYYLNKDLDQMTEDDFDNILESLERNKLTDYNYRKT
                     IKKFFRWHTNENVPKWVKDIKMPLTKTPVQPQDLLTKDEVNRLLNACGNPRDKAMISI
                     ALDSSLRIGALGTLKIKSIAQNKSGAVLYISPTSKNIKSTQPKPIPITWSVGYLNKWL
                     DVHPLKDNLDAPLWVNLHGKTRNQLMSYKIIRQSLCNVAETAGIKKRVFFHLFRHQKI
                     TDMILNGFSEQQIKYQAGWAKGSSRMLDIYGNFMDADMVDSIFEKQGLKSRDENSKQV
                     TLTKCPRCDVVLISEARVCHQCALILDPGLDKELKVGEDQAQKALLKMMDSPEVRALL
                     KEMMNK"
     gene            complement(1572..1644)
                     /locus_tag="VKL21_12355"
     tRNA            complement(1572..1644)
                     /locus_tag="VKL21_12355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1608..1610),aa:Gln,seq:ctg)
     gene            complement(1711..2817)
                     /locus_tag="VKL21_12360"
     CDS             complement(1711..2817)
                     /locus_tag="VKL21_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HMB46601.1"
                     /translation="MQHMAPMPGRNWYNIIFNISSFFTTLSVDKTRTGIVYHPDYLRH
                     DTGHHPERKERLLSIISHLKETGMIEKLELTKPRPASLEEIRYIHSEKYIEKARRHSQ
                     LEMNLDPDTVLSRDSYDVALLAAGGAITAVDSVLDKLNSSFALVRPPGHHAMPGMGMG
                     FCIFNNIAIAARHAQKKGKKRVLIVDWDVHHGNGTQDAFYDDPSILYFSTHEYPYYPG
                     TGWLDETGTGQGEGYNINVPLPAGTDDSGFIAAFEEILVPAAREFHPEMVLISAGQDA
                     CNEDGLAGMKMSVKDFSALALIVRSIANESCAGKVAAVLEGGYNLNLLARSVAEVLDV
                     FMGNEAEKKSHEISPQVRTRLDEVKKIQQEFWHL"
     gene            complement(2777..3175)
                     /locus_tag="VKL21_12365"
     CDS             complement(2777..3175)
                     /locus_tag="VKL21_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMB46602.1"
                     /translation="MVKMPSEIKDIVAKQKPLPIATADKSGKPNVVFVTMWKILDDET
                     ILFVDNFFNKTRKNIEANPNMAIVAYDGDAKKSYQIKGTVDIENKGDRYTSAKEMADS
                     KKLPGKSAFVFHVKEIYDATYGPNAGKKLV"
     gene            3319..3392
                     /locus_tag="VKL21_12370"
     tRNA            3319..3392
                     /locus_tag="VKL21_12370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3353..3355,aa:Thr,seq:tgt)
     gene            3579..3803
                     /locus_tag="VKL21_12375"
     CDS             3579..3803
                     /locus_tag="VKL21_12375"
                     /inference="COORDINATES: protein motif:HMM:NF025469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4277 domain-containing protein"
                     /protein_id="HMB46603.1"
                     /translation="MNETVTKPNSQAIVDTSTMISGMILNLLSDAKIRLYRLYHFFDD
                     KPMPLIFLWKPNLTPSNFTEERTGNVLDDG"
     gene            3993..4346
                     /locus_tag="VKL21_12380"
     CDS             3993..4346
                     /locus_tag="VKL21_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46604.1"
                     /translation="MAKSLVGAYCLKNPERIVAFGYILLIAAQVYTILERVVRKNLEN
                     PDEEPVEGLNRQKTKRPTAYAIEYVLSAILVMRVQKKKCEEWILSKELDKNQKRMLNL
                     AGFDERIYCKSLRTN"
     gene            4427..4564
                     /locus_tag="VKL21_12385"
     CDS             4427..4564
                     /locus_tag="VKL21_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46605.1"
                     /translation="MQSFVVEQTEVRTIRRRFFIEAEDEEEAIDKMQSKQVDEVPEYT
                     K"
     gene            4729..>5005
                     /locus_tag="VKL21_12390"
     CDS             4729..>5005
                     /locus_tag="VKL21_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HMB46606.1"
                     /translation="MSDITFQIMFIIFLTIANGVFSMSEIAIVSARKARLQQWVNEGN
                     AKARVALDLANSPNRLLSTVQIGITLIGILAGVLGGATIAEELSARLS"
BASE COUNT         1437 a         1035 c         1024 g         1509 t
ORIGIN      
        1 cccttaaaga actcggagaa attgccgaga cattaataaa aaaagacagg cctgaacatt
       61 ggaaaaacga attgggggat ttgtgtggcc tgtgtatcaa acctatgcta gaactggctg
      121 agacagactt cgatactgca tgccaggtag gtataaaccg aagacagcaa aagatgcacg
      181 aaaatcatat taaaaataat ggagcctcag ctccataact gatggacgtc gcatttcgtg
      241 cgacgcctcg aatattaatc aatactcaag aacacgagcc aagtgcccgg ctctggagca
      301 aacaaaatca gatcatggta ttgatcactt attcatcatc tccttgagga gtgccctcac
      361 ttccgggcta tccatcattt ttaggagtgc tttctgggcc tgatcttccc cgacctttag
      421 ctctttatcc agacctggat cgagtatgag ggcgcattga tgacaaaccc gtgcttccga
      481 aatcaggaca acatcgcatc gagggcactt tgtaagtgtg acttgtttgg aattttcatc
      541 tcttgattta agtccctgct tttcaaatat cgagtcaacc atatcggcat ccataaagtt
      601 cccatagata tctaacattc ttgaacttcc tttagcccaa cctgcctggt acttaatctg
      661 ttgttcactg aatccattga ggatcatatc ggtgattttc tgatgcctga atagatggaa
      721 aaaaactctt ttcttaatcc cggcagtttc cgccacgtta cagagtgatt gacggatgat
      781 cttgtatgac attagctggt tccgtgtctt cccatgaaga ttgacccaaa gtggtgcatc
      841 cagattgtct tttaggggat gaacatcaag ccatttattt aaatagccta cagaccatgt
      901 tatggggatc ggcttcggtt gtgttgactt gatattcttg cttgtcggac taatgtataa
      961 tactgcacca gacttgtttt gagcaatact ttttattttc aaagtgccca aagctccaat
     1021 tcgcaaggaa ctgtcgagtg ctatagaaat catggcctta tcgcgaggat tcccacaagc
     1081 gtttaaaagc cgatttactt catcttttgt gagaagatct tgcggttgta ctggcgtttt
     1141 tgtcaatggc atcttgatat cttttaccca tttcggaaca ttttcgttag tatgccacct
     1201 gaaaaacttc ttgattgtct ttctataatt atagtctgtt aatttattcc tttcgagact
     1261 ctcaaggata ttatcaaaat catcttctgt catctgatcc aaatctttgt taagataata
     1321 tcttgatatg atttgaatga aaatagcgca gtttgctgca agtgtacttt tccgattccc
     1381 ctttcttttg tgagttttta agaaatctgc tacgagttgt ttgttacggt ctgatgtctt
     1441 gactttattg aacttagcca ttgattgaga gagcttaaga tcaaatccat gaatatcatc
     1501 ggtattttca ttatacgcca ttaatgtctc ctttcatata ttggcgctta ggattataac
     1561 catttagagg atagcccggc aaggattcga accttggtcg caggatccag agtcccgcat
     1621 gattgaccgc tacactaccg ggcttcgaac ggcctcctaa atattagctt tccacgtatt
     1681 aatctatcga ttgcccaatt cttagatatt ttagaggtgc cagaattctt gctggatttt
     1741 cttgacttca tctaaccgcg ttcttacctg cggacttatt tcatgcgatt ttttttcagc
     1801 ttcatttccc atgaatacat ccagaacttc tgcaacagag cgtgcaagaa gattaaggtt
     1861 gtaacctccc tcaagaaccg ctgcgacttt ccctgcgcag ctttcgttgg caattgaccg
     1921 tacgatcaat gcaagcgcag aaaagtcttt cacgctcatt ttcatgcctg caagtccatc
     1981 ctcattgcat gcatcctgac ctgccgatat caaaaccatc tccggatgaa attcacgcgc
     2041 tgcaggaaca agtatttctt cgaatgccgc aatgaatccc gaatcatctg ttcctgccgg
     2101 caggggcaca ttgatgttat atccctcgcc ctggccagta ccagtttcat cgagccatcc
     2161 tgttccaggg taatagggat actcatgagt ggaaaaatat aatatggaag gatcatcata
     2221 aaaggcatcc tgtgtgccat tgccgtgatg cacatcccag tcaacgatca gcacgcgctt
     2281 tttccctttt ttctgtgcat ggcgcgccgc aattgcgatg ttattgaata tgcaaaaacc
     2341 catccccatc cccggcattg catgatgtcc tgggggtctg acaagagcaa atgacgagtt
     2401 aagtttatcc aggacagaat ccacagctgt tatggcgccg cctgccgcaa ggagcgccac
     2461 atcgtaagaa tccctggata aaacagtatc agggtcaagg ttcatctcaa gctgggaatg
     2521 tcttctggct ttttctatat acttttcaga atgaatatac cggatttctt caaggctggc
     2581 gggcctgggt tttgtcaatt ccagcttctc tatcatacct gtctctttca aatgagatat
     2641 tatcgaaagc aggcgctctt tcctctcagg gtgatgtcct gtatcgtgtc tcaggtaatc
     2701 aggatggtaa acgatacccg ttctggtttt atcgactgac aaagttgtaa aaaaagaaga
     2761 aatattgaat ataatattat accagtttct tcccggcatt ggggccatat gttgcatcgt
     2821 atatctcctt tacatggaat acgaatgctg actttcctgg caatttcttg gaatctgcca
     2881 tctcttttgc gcttgtatac ctgtcccctt tattttcgat atctacagtc ccttttatct
     2941 ggtaggattt ctttgcatcc ccatcataag ctactatcgc catatttgga tttgcttcaa
     3001 tgttctttcg tgtcttgttg aagaaattat ctacgaataa aattgtttca tcatcaagta
     3061 tcttccacat cgtaacgaac acgacgtttg gtttcccact cttatcagca gtcgctatag
     3121 gcaatggttt ttgcttcgcg acaatatctt ttatttcaga aggcattttc accataaatt
     3181 atcacagtta ttcgtttagt ttgcatttat cataaagttt atgatatgat tttcctgagt
     3241 tcttcatata ttataaaaaa ttaattaccg aaacttttaa gtctgattcc atccttaaaa
     3301 gcgaaagctt tttaataagc ctcggtggct cagcttggca gagcgagaga tttgtaatct
     3361 ctaggtcgag ggttcaaatc cctcccgggg ctctctttaa ccacatctaa agcctacttt
     3421 ggaatttata agcagagcag acaaagaggc taaaagcatg gttaaggaag acatacacaa
     3481 tttgaaaggt attctggaaa gagatactga gcgctatcta ttaacccgca ttccgtaccc
     3541 taagtatatt tagaaatcta tttttaccaa tagcgataat taatgagacc gtgacaaaac
     3601 caaacagtca agcaatagtt gataccagta caatgatttc aggcatgatt ttgaatcttt
     3661 tatcagatgc caaaataagg ctatatcgcc tgtatcattt ttttgatgat aaaccgatgc
     3721 cattgatctt tctctggaaa ccaaacctga ctccatccaa tttcaccgaa gagcgtacag
     3781 gcaatgttct tgatgatgga taaaaaccgt ccgtcaggct ttacaaaaaa gtgatgatga
     3841 cttcttgctc tatacagcag atagtgcagc ggttacagaa gagaatctta aactattcaa
     3901 agaatatcat gtagatatga tttcccggat tacatctgaa attattgagg ttgaaaaaat
     3961 tattaaaaga aataagagag gacgtcttaa gaatggcgaa atccttggtg ggagcttatt
     4021 gcttgaagaa tcctgaacga atcgttgctt tcggttatat tcttttgata gctgctcagg
     4081 tctatacaat tctggaaaga gtagtcagga aaaatctgga aaatcctgat gaagagccag
     4141 ttgaagggct taatcgacag aagacaaaaa gaccaacagc ttatgcgata gaatatgttc
     4201 tgagtgctat tcttgtgatg agagtacaaa aaaagaaatg tgaagaatgg atactatcta
     4261 aagagctgga taaaaaccag aagagaatgt tgaacttggc gggatttgat gaaaggattt
     4321 actgtaaatc attgagaacg aattaattgg gcaaaagtaa aatgaaaagg tacggaatgc
     4381 aggttttaag gtactgcgct actcaatcag acagccagcc tgaattatgc agagttttgt
     4441 tgttgaacaa actgaggtaa ggacgatcag gcgcaggttc ttcatagagg ctgaagatga
     4501 ggaagaggcg atcgataaga tgcagagcaa gcaggtcgat gaagtcccag agtacacgaa
     4561 gtaggatctt aaggaactaa atttcgatgt tgagctgtta ggaataaatc atgagtacag
     4621 gggagctaaa atgcaagaac agctcgttag ctaattcaga taagccaccc actgcaaaaa
     4681 ggatttaaca aggaaaattt aatatcaagc taataagagg ttgcaggaat gtccgatatc
     4741 acttttcaaa ttatgttcat tatctttctt acgattgcca atggtgtttt ttccatgtct
     4801 gagattgcta ttgtttcggc gcgcaaggcg cgactccaac aatgggtcaa tgaaggcaat
     4861 gcaaaggcac gcgtcgcgtt ggatcttgca aattcaccca atcgtttact atccacagtt
     4921 caaattggca tcacactcat cggcatactg gctggcgtat taggcggagc aaccatagcg
     4981 gaggaactat cggcacgctt gagct
//
LOCUS       DATGBN010000484         5469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_271895, whole genome shotgun sequence.
ACCESSION   DATGBN010000484 DATGBN010000000
VERSION     DATGBN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271895"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..305
                     /locus_tag="VKL21_12395"
     CDS             90..305
                     /locus_tag="VKL21_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46607.1"
                     /translation="MEKKTGIYSLVASIVTSIVIVLIFYILAAGKVSANPEMYKPVDI
                     YAGMVYVFVLSMIVSASIWPGIIENNT"
     gene            332..610
                     /locus_tag="VKL21_12400"
     CDS             332..610
                     /locus_tag="VKL21_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46608.1"
                     /translation="MTNITLSIPNDVHFKMKMHKEIRWSEVVRMAITDFIGKLEEKGS
                     GMTTIELLEELGEDFRKNLSELSFETAEKEFKKMRDAEWKRVSTIQAG"
     gene            577..903
                     /locus_tag="VKL21_12405"
     CDS             577..903
                     /locus_tag="VKL21_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46609.1"
                     /translation="MEESLYDTSRLIKAYKEKETILGYTTIFNLIEFPKAIEFNLRVL
                     FPSKSDYYLSLRISTELLKSEKPIPAVDSLIAAIVLNNKLKLVTRDHHFLLIEKIMKD
                     FKVDVV"
     gene            1016..1423
                     /locus_tag="VKL21_12410"
     CDS             1016..1423
                     /locus_tag="VKL21_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46610.1"
                     /translation="MNRWKTFALIMIGLLAVAVGFRFTYFEGHSFPVDEKERLFAINA
                     AQNGLKDENGINYYNITVQERGRIISTLNGDKKVLAVVLTRENITLTALVDMETGNVV
                     EKSRIESSGWLAEYIKNRNNNSKGWGPQRLFNR"
     gene            1428..2105
                     /locus_tag="VKL21_12415"
     CDS             1428..2105
                     /locus_tag="VKL21_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB46611.1"
                     /translation="MEVIMELQDVWKIYHMGEFDVPALAGIGLAVKKGEFVAITGPSG
                     CGKSTMLNMIGCLDMPTRGKVLLEGRDISEFKSNELARVRGKKIGFIFQTFNLYPTLT
                     ALENIRLPMRIHEFPEKDVEDTSIKLLEMVGLSERGSHFPAQLSGGQQQRVAVARALS
                     TGPSILLADEPTGNLDTKSGGEVMDVFKGLNNEGLTIVMITHDAKIAGAAGRVVKMLD
                     GKIVGET"
     gene            2102..3298
                     /locus_tag="VKL21_12420"
     CDS             2102..3298
                     /locus_tag="VKL21_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46612.1"
                     /translation="MNKSNLILILVSTLMIAQVASAATNYLVTESAPVYPGDTTYVYV
                     PIKNMGFGSFMEDVTVKLAPKENASANAVTILNDVDSLGTISNWGDQRTAKFRIYVNP
                     DTVEGDYYFNVYVTYKGQVTSSGAPTMSATTKLDDQILTIRGKPMIMMVNSTIGIVAP
                     MSITRETLQFKNAGTGTVQNAVAEINIASANSAFSILGGGKQFFLGNLKAGDEASITF
                     DLAVDIAARPGVYNIPVTITGQNNYSADNIIGLVVAGITDFDISYVETLGSFSLNVAN
                     VGISTASAVAVNIPPQKNFSVAGSSTSALGNLNPGDYTPAIFQLTKTPGAGNDLELEI
                     QYTDTSGQRHTIAKSLHVALSATGSQTKSTSTNYTTWLLVAVIILVLYLQRGKISKYF
                     RKPEGK"
     gene            3326..4579
                     /locus_tag="VKL21_12425"
     CDS             3326..4579
                     /locus_tag="VKL21_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB46613.1"
                     /translation="MKTLDTFRLSLSHVRKSKMRSWLTIIGIVIGVAAVVAIISIGQG
                     MQESVAARLGSLGADLITVTPGFSRASSGGGFEGGRSGGSTSINLTDKDVNVIKQVPG
                     VLYVNGMVSGRSDMIMGTEKTSVSITGVDTVVWRSMVTTGLEAGRYLQPGDSNAVVIG
                     YSLAHETFLQPLTLNRPVTIGGKTFKVAGIFVQSGGGFGGGGDNAVYMPADYARDVIT
                     TNISRNAFSSIMVKVTDPNLANQVASDIITKLMPSRHVNSRTRDFTVTEFAVIQQQIT
                     SVVQTISLFLAAIVAVSLIVGAVGIANTMFMSVMERTRQIGLLKALGATDAEVMKLFL
                     MESGLFGFVGGVIGIITGVLISVIISAVGLIAIGPGGAMSAVVSPQLLIFALAFSIFV
                     GIISGVAPARSAAKMNPVDALRFEQ"
     gene            4617..5318
                     /locus_tag="VKL21_12430"
     CDS             4617..5318
                     /locus_tag="VKL21_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB46614.1"
                     /translation="MMETIMKLQDVWKIYRMGEVEVPALKGVSVEIKKGDFVAIMGAS
                     GSGKSTMMNLIGCLDIPSRGSIYLKSKDISTLSESDLASFRGKTIGFIFQQYNLIQSM
                     SAYENVMLPLEFLEYDDQKAATRTREILSLVGLGDKMHHRPTQLSGGQQQRVSIARSL
                     VSDPEIILADEPTGALDSVTGKDVLDMLHRLWKEHGKTIILVTHDLNLAKYAHTTIEL
                     KDGEILRISQNHEVN"
BASE COUNT         1683 a         1019 c         1353 g         1414 t
ORIGIN      
        1 cgcttgccgc ggcagcgatg gctgcaagtt cggttacggt cacaatgaat acgctcctta
       61 tgaagcgcat aaaattgcag gagtgaatta tggaaaaaaa aactgggatt tacagtctgg
      121 ttgcaagtat cgtaacttcg atcgttatag tgttaatttt ctatatcctt gccgccggca
      181 aagtgagcgc aaatcctgaa atgtacaaac ctgttgatat ctatgcggga atggtgtatg
      241 tatttgtgct ttccatgata gtgtcggcat cgatatggcc cgggatcatt gaaaataaca
      301 cttaaatacg tgaattcaca tatataagaa tatgacaaat ataacacttt caataccaaa
      361 tgatgtgcat ttcaaaatga aaatgcataa agagatccgt tggagtgaag tggtaagaat
      421 ggcaataact gatttcattg gaaaacttga ggaaaaaggt tccggaatga caacgattga
      481 attactcgaa gaacttggcg aagatttcag aaaaaacctg agcgagttga gttttgagac
      541 ggcagaaaaa gagtttaaga agatgaggga tgcagaatgg aagagagtct ctacgataca
      601 agcaggttga tcaaagccta taaagagaag gaaaccatac tgggttatac cacaatattc
      661 aatctcatag agtttccaaa agccatagaa ttcaatcttc gagtgctgtt tccttctaaa
      721 tccgactact atctgtcttt aagaatttct acggaacttt taaagtccga aaaaccaata
      781 cctgctgtag attcattaat tgcagcgatt gttttgaata ataaattaaa gctggtgacc
      841 agagatcacc attttctcct gattgaaaag attatgaaag atttcaaagt tgatgttgta
      901 tgatatttga gaattctaaa caaaagatag ctcaggctta atttcatttt tttgaaactg
      961 ctttcaggat aatttcagga aattcttata tggtctttca tccagtggtg gttccatgaa
     1021 ccgatggaaa acattcgcat tgatcatgat cggcctgctg gcagttgctg tagggttcag
     1081 gtttacatat tttgaagggc attcattccc tgttgatgag aaagagaggc ttttcgcaat
     1141 aaatgcagcc caaaatggac taaaagatga aaatgggatt aattattata atattacagt
     1201 acaggaacgt gggcggatta tttccaccct gaacggggat aaaaaagtcc tggctgttgt
     1261 tcttaccagg gaaaatatca ccctgactgc tcttgttgat atggaaaccg gaaatgtagt
     1321 tgaaaaaagc aggatagaat ccagcggatg gctggcggaa tatataaaga atagaaataa
     1381 taattcaaag ggctggggtc ctcaacgatt atttaaccgg tgatttgatg gaagttataa
     1441 tggaattgca ggatgtatgg aaaatttatc acatgggtga gtttgatgta ccggcgcttg
     1501 cgggaatagg cctggcggtc aaaaaaggtg agttcgtggc cataacagga cccagcggct
     1561 gcggtaaatc aacgatgtta aatatgatag gttgtcttga catgccaacg cgcggaaagg
     1621 ttttgctcga aggcagggac atatcggaat ttaagagtaa tgaactggcg cgtgtaagag
     1681 gtaaaaagat cgggttcata ttccagactt ttaacctgta tcctaccctt acagctcttg
     1741 agaatattcg actgccaatg aggattcatg aatttcctga aaaagatgtg gaagacacat
     1801 caataaaact gcttgaaatg gttggtttgt cggaacgagg gagccatttt ccggcccagt
     1861 tatcaggtgg acagcagcaa agagtagcgg tcgcaagggc gctgtccaca ggcccttcga
     1921 tcctcctggc agatgaaccc acggggaacc tggataccaa atcaggaggc gaagtaatgg
     1981 atgtttttaa aggattgaac aatgagggat tgactattgt tatgataaca catgacgcaa
     2041 aaattgcagg agctgcagga agagttgtga aaatgctgga tggaaaaata gtaggtgaaa
     2101 catgaataag tccaatttga ttttgatatt agtatcgaca ttaatgattg cacaggttgc
     2161 atcagcggcc actaattatc ttgtgactga aagcgcgcct gtttatccgg gggatacgac
     2221 atatgtctat gtgccgataa agaacatggg atttgggagc tttatggaag atgttactgt
     2281 aaaactcgcg ccaaaggaaa atgcttcggc taatgccgta acgatattga atgatgtaga
     2341 ttcactggga accattagta attgggggga ccagagaacc gcaaaattca gaatatatgt
     2401 aaatcctgat acagtagaag gagattatta tttcaatgtt tatgttacct ataagggaca
     2461 ggtaacgagc agcggggctc cgacaatgtc tgctacaact aagttagatg accagatatt
     2521 gaccatcagg ggaaagccca tgataatgat ggttaattca acgatcggca ttgtagcgcc
     2581 catgagcatt acacgggaga cgcttcaatt taaaaatgca ggaacaggta cagtacagaa
     2641 tgctgtggct gaaataaaca ttgccagcgc taattcggca ttttccatac ttggaggagg
     2701 aaaacaattt ttcctgggta atttgaaagc aggtgatgaa gcatcgataa ctttcgatct
     2761 tgctgtagat atagctgcgc gccctggagt atataatatt cccgtaacga tcacaggaca
     2821 gaacaattat tcagcagata atatcatcgg attggtagtc gcgggaatta cagattttga
     2881 tataagctac gtggagactt taggatcttt ttcactcaat gtcgccaatg tggggataag
     2941 caccgcaagt gcagtggcag tcaacattcc cccccagaag aacttctccg ttgcagggag
     3001 cagtacttct gcgcttggga atctaaatcc aggggattac acaccggcga tattccagtt
     3061 aacgaaaaca cctggcgctg gaaatgatct tgaactggag atacaatata ccgacaccag
     3121 cgggcaaaga catacgatag caaagtctct tcatgtggca ttatccgcaa caggatcaca
     3181 aacaaaatct acaagtacaa attatacgac ctggttgctg gttgctgtga tcatactcgt
     3241 attatacctt cagcgaggaa aaatatcaaa atatttccgg aaacctgaag gaaagtaaac
     3301 caaaggaaac caataggtac taaatatgaa aactttggat acattcaggt tatccctgag
     3361 ccatgtcagg aaaagcaaga tgcgaagctg gctcactata ataggcattg taataggtgt
     3421 ggctgcggtc gttgccataa tctccatagg acagggtatg caggagagtg tggcggcccg
     3481 ccttggcagt cttggcgccg atcttatcac tgtcaccccg ggtttttcac gggcaagcag
     3541 cggtgggggt tttgagggtg gtcgatcagg tggaagtaca agtattaacc tcactgataa
     3601 agatgtgaac gtgatcaagc aggtacctgg tgtgctttat gtaaacggca tggtttcagg
     3661 caggtctgat atgataatgg gaacggagaa gacatcagtg tccataacag gagttgatac
     3721 cgtagtctgg cgctccatgg tcacaaccgg gcttgaagca ggcagatacc tccaaccggg
     3781 tgattcgaat gccgtggtca taggttactc tcttgctcat gaaacttttt tgcagccgct
     3841 caccctgaac aggccagtta ccattggagg aaagactttc aaggtcgcgg ggatcttcgt
     3901 ccaatcagga gggggttttg gaggcggagg cgataatgcg gtgtatatgc cggctgatta
     3961 tgcacgagat gtgataacca cgaatatatc caggaacgca ttttcttcga taatggtaaa
     4021 ggttaccgac cccaatctgg caaatcaagt agcgtcagac attataacaa aacttatgcc
     4081 atcgaggcac gtcaattcaa gaaccaggga ttttacggtt actgaatttg cggtaataca
     4141 gcagcagata accagtgttg tccagaccat atcgctattc ctggctgcca tagttgcagt
     4201 ttctctaatc gtgggcgctg taggcatagc gaataccatg ttcatgtcag tcatggaacg
     4261 cacaaggcaa attggattgc tcaaggctct tggagccaca gacgccgaag ttatgaaact
     4321 tttcctgatg gaatccggat tattcgggtt tgtgggaggc gtgatcggaa taatcaccgg
     4381 tgtactgata tcggtcataa tatcggctgt gggcttgata gcgataggac caggcggagc
     4441 tatgagcgca gttgtatcgc ctcaacttct tatctttgca cttgcattct caatatttgt
     4501 ggggataata tcaggtgtcg cgcccgcacg gtcagcagca aagatgaacc cggtggatgc
     4561 tcttcgattt gaacaatagt atggttttgg attctttttg ctagacggaa aaataaatga
     4621 tggaaacaat aatgaaattg caggatgtct ggaaaattta caggatgggg gaagtggaag
     4681 taccagcatt aaaaggagta agcgttgaaa taaagaaagg agactttgtg gccatcatgg
     4741 gagcgtcggg aagcggcaag tccacaatga tgaacctgat aggctgcctg gacattccct
     4801 ccaggggaag catctacctc aaatcaaaag atatcagcac attaagcgaa tctgacctgg
     4861 cgtcattccg gggcaagact ataggtttca tcttccagca gtataacctg atccagtcaa
     4921 tgtctgctta cgagaacgtg atgcttcccc tggaattctt agaatatgac gaccaaaaag
     4981 cagcgacaag gacaagagaa atattatcac tggttggcct gggcgacaaa atgcatcaca
     5041 ggccaacgca gctttcagga gggcagcagc aaagggtgtc catagcgaga agcctggtaa
     5101 gcgatcctga gataattctt gcagacgaac ccaccggagc actggacagt gttacaggga
     5161 aagatgtcct ggatatgctt cacaggttat ggaaagaaca tggaaagacc atcatccttg
     5221 tcacgcacga ccttaatctt gcaaaatatg cccatacgac tattgaacta aaagatgggg
     5281 aaattttgag aatatcgcaa aatcatgagg tgaattaata atgaaacaaa caattgtatg
     5341 ggtttttatg ttcctggcat tgatggtttg catccctgca gttaatgcag aactgggaag
     5401 tttagtggat acaacggtga tttcaattag ccttgcaaac tatgatcctg accctgctat
     5461 gggggggaa
//
LOCUS       DATGBN010000485         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_38033, whole genome shotgun sequence.
ACCESSION   DATGBN010000485 DATGBN010000000
VERSION     DATGBN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38033"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..547
                     /locus_tag="VKL21_12435"
     CDS             134..547
                     /locus_tag="VKL21_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMB46615.1"
                     /translation="MTPTEDLINEHKAIKVMLSIMSKIAENIKANKGYYIKDVEQIVD
                     FLKTFADKCHHGKEENVLFPALVLAGIPKENDPIGVMLQEHTLGKGYIKEINRGLENC
                     KLGDTCSSELIAANLQNHMIEKFFCVSLSYLLSHS"
     gene            complement(687..1916)
                     /locus_tag="VKL21_12440"
     CDS             complement(687..1916)
                     /locus_tag="VKL21_12440"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HMB46616.1"
                     /translation="MNKKKSGWKTRHLLLSLLSLKNPPLMKTNLSIFAQIIRLIPRDI
                     IHKNVDEHDSDFNSKGINSWTHLVSMLYCQFGNCQSIRDISFGLRSATGNLSHLGVCR
                     APSKSSISYINAHRGWTFFRDVYFSLFKEFKVKVNRLRLNRKLFILDSTLIPLCLSLF
                     DWAYYRKMKGAIKIHTVLDYDSCLPVFIRMTDGKTSDIEIAEKLAFPKGSILTIDRGY
                     LDFNFLRELDSSGVFFVTRAKSNIDCDIVKLLALPNESENIVSDYQIVLIGQQTKGKY
                     KKRLRLVKVTDPETGEIIPLLTNNFTWTSKTISELYRYRWEIETFFRLVKQNLKIKSF
                     IGTSENAVLIQIWSALITILLLSVLKTKALYKWHMSNMVGFIRLNLFVKIDLFEWLNH
                     PFLQDKAPPGQIQLDLF"
BASE COUNT          692 a          370 c          347 g          627 t
ORIGIN      
        1 cacctaatcc gtctttatct cagaacggag atatggtcag aaagattcac aaagcctaat
       61 gaaaaagtga gtcatccgat taaaaagaac aattcctgta atcagttgtt aaaaaactta
      121 aaaaatcaga attatgacac caacagaaga tttaataaat gaacataaag caattaaagt
      181 gatgcttagc attatgagca aaattgctga gaacataaaa gcaaacaaag gatattatat
      241 aaaagatgtt gagcagattg ttgatttcct aaaaacattt gctgataaat gccaccatgg
      301 taaagaagaa aatgttctgt ttcctgctct tgttttagca ggaataccaa aagaaaatga
      361 tccaattgga gttatgctcc aggaacacac gcttggaaag ggttatataa aagaaataaa
      421 cagaggcctg gaaaactgta aattaggcga cacctgttca agtgaattaa ttgcggcgaa
      481 tctgcaaaat catatgattg agaagttctt ttgcgtttcc ttgagttatt tattatctca
      541 ctcataaaat ttattattta ttaaagataa aattaagaat aatcagtgct gtccaagata
      601 aataataact ctgagttcta atccagatta tcagacaatt aactgttata tttattaaaa
      661 tcgtaaaacg atccctatcc aggttctcaa aacaaatcta actggatttg cccgggtggt
      721 gctttatcct gcaaaaatgg atggtttaac cactcaaaca gatcaatctt aacaaaaagg
      781 ttaagccgga taaatcctac catgttagac atatgccact tgtataaagc tttcgttttc
      841 aggacagata ataacaaaat ggtaatcaac gccgaccata tctggataag aactgcattt
      901 tcagaggtcc cgatgaatga ttttatcttg agattctgtt tgaccagtct gaaaaaagtc
      961 tctatctccc agcgatatcg gtacaattcg cttatagtct tactcgtcca ggtgaagtta
     1021 ttcgttaata atgggattat ctccccggtt tctggatcag ttaccttcac caaacgtaac
     1081 ctttttttat actttccttt ggtttgctgt ccaataagca caatttgata atcactaaca
     1141 atgttttctg attcattcgg tagtgccagc agcttaacaa tgtcacaatc tatattgctt
     1201 ttggctcttg taacaaaaaa caccccgctg ctgtccaatt cccgaagaaa attaaaatca
     1261 agatatcctc tgtcgattgt cagtatgctg ccttttggga aagccagctt ttcagcgatt
     1321 tcaatatcac tggtcttccc atcggtcatt ctaataaaga cagggagaca gctatcgtaa
     1381 tcaagaacgg tatgtatttt aatggctcct ttcattttcc tgtaatatgc ccaatcaaac
     1441 agacttaaac acagcgggat cagagtagaa tcaaggataa agagcttcct gttgagtctt
     1501 aacctattaa cctttacctt aaactcttta aaaagactga agtaaacatc acggaaaaaa
     1561 gtccagcccc tgtgggcatt aatgtagctt atagaagatt ttgaaggggc tctgcacact
     1621 cccaaatggc tcaggttacc ggtagcactt cttaatccaa aactgatgtc ccggatactc
     1681 tgacaattac caaactgaca atataacatt gaaacaagat gtgtccagga atttattcct
     1741 ttagaattaa agtcagaatc gtgctcatca acatttttgt gaataatatc tcggggaatt
     1801 agcctgatta tttgagcgaa aatggataaa tttgtcttca tgagcggtgg gtttttgaga
     1861 gataataaac ttagcagtaa atgcctagtt ttccaaccgc tctttttctt attcaatttt
     1921 acttattttg gacagatctg attattactg aaaaaaatct taatcatgta ctttaaaata
     1981 tttagtcgtt aaaattggat ttgcagcagt ataaggtata aatatggata tcacct
//
LOCUS       DATGBN010000486         6626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_193794, whole genome shotgun sequence.
ACCESSION   DATGBN010000486 DATGBN010000000
VERSION     DATGBN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193794"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VKL21_12445"
     CDS             complement(<1..734)
                     /locus_tag="VKL21_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB46617.1"
                     /translation="MKIKNIMCEPFTVHKSDKISHAMDLMDKHDTRRLLVINGSEAMG
                     VITMRSIADKLGTWKTSNLPASSLHVAAATTDKFAKVLPDMGIEDAIVLMDRNGGILI
                     AMENSKILGWVTPHEIMKNIRGVKGYAAQIMKEPITVSPGDRVSHGRRIMLDNDIGRL
                     PIVENGDIVGIITERDIAKAMMNFRDLVPDNQQDERIRNLIVGDIMTRNVISVRTNTP
                     ITEVISTLLDKNIGGVPVLNLKDEMVG"
     gene            complement(744..1592)
                     /locus_tag="VKL21_12450"
     CDS             complement(744..1592)
                     /locus_tag="VKL21_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB46618.1"
                     /translation="MKIKDIMSAPVYVVSPDETVARARNLMLRHKIGRLVIIEDNKPI
                     GMVTKKDIVKRLNQAEPQWRRRPIDDIPVRKVMTESLITIFPDATPRQLAELMTENNI
                     GGLPVVNNKDEVIGIATKWDLIRYFSKLELDLKVKDMKIEPALTVHRHHTISHIIHEL
                     QANSIDRAIVLEDNDKPVGIITNSNLTFTEMKDKTGGLPQKVIKMTRKDSSAGRKQNR
                     YVDEVPLVAEDIMSSPIVNVNSEELATVAAGLMVKERINGLSVIGNGVMGILTGENII
                     KTIMTM"
     gene            complement(1602..2537)
                     /locus_tag="VKL21_12455"
     CDS             complement(1602..2537)
                     /locus_tag="VKL21_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB46619.1"
                     /translation="MNKSGNNIRIEKQGRKGYMRNMAPIDFKSRISEKSGDIMSIASN
                     DVITIPPTMPVIEAVKTMLNHGFRRIPIADAGTKHLLGIVTSNDIVDFLGGGKRNLII
                     KNKFKGNLLAAVNGSISEIMETNVVSLNVKDSLKDALNTMIKEKIGGIPITDGDGAVK
                     AIISERDFVFLMSGIATGKTVEGYMSKKIVTAPSDMSIGAAARSMINNGFRRLPILRD
                     NVLIGIITASDIMRFLGSGDMFDRLVTGNAREVFEVSVSTLIQRDIIFTKSDIDLGEA
                     ANVMLDKDVGSLPVLEEGELKGIITERDFVRAMAN"
     gene            complement(2548..3321)
                     /locus_tag="VKL21_12460"
     CDS             complement(2548..3321)
                     /locus_tag="VKL21_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB46620.1"
                     /translation="MKVKDVMTRNPVFLQDTDFMTHARQVMRDRHYRTMPVLDSKNRV
                     LGILTEKELLNIYSTKSNVTVEGFTSEFPFIYPEMDMMDAAKLMMDAGVGRSPVLKSG
                     QEMTLAGILSVVDIFRNLKVDKIPQKKLHEIMTRDVKTCSNKDHISKVWLNMKETGIS
                     GFPVLKNGELVGMVTRRNIINAGYARIEREDNQGTKSTMSPPVEKIMSTPAYTLSSEA
                     TLAEAIQSFLKLDVGRISITNEGKLVGIVDRNDIIKAFI"
     gene            complement(3393..3941)
                     /locus_tag="VKL21_12465"
     CDS             complement(3393..3941)
                     /locus_tag="VKL21_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMB46621.1"
                     /translation="MEVKMPVKEIMTGDVVTVDIKSDVRQLAVKMLTFKVGSVIVTDN
                     KQPVGIVTERDIVKKIVSKNLRPDEISIKELMTTPLISISSSEDVSETMHKMVKMEIR
                     RLPVVENGKLVGIITDTDLLAVSAEMGSIYSDLIKMHREKLFTMETEGRVFKGICEDC
                     GYISDNLLAIEGKLLCESCRET"
     gene            complement(4017..4427)
                     /gene="ribH"
                     /locus_tag="VKL21_12470"
     CDS             complement(4017..4427)
                     /gene="ribH"
                     /locus_tag="VKL21_12470"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMB46622.1"
                     /translation="MTIKLGFVVSEFNRDLTIQMETLGKEHAAFLGATVEKIYYVPGV
                     FDMPLAVKKMAQDKTIDAVVTIGCVIQGATKHDELVIQHASRKIADLALEFSKPITLG
                     ISGPGMSRLDAHERVEYGKRAVEAAIKMVRMLKQ"
     gene            4559..5554
                     /gene="ilvC"
                     /locus_tag="VKL21_12475"
     CDS             4559..5554
                     /gene="ilvC"
                     /locus_tag="VKL21_12475"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HMB46623.1"
                     /translation="MVKIYYDKDADLKVLKKKTIAVIGYGSQGAGQAQNLHDSGLNVI
                     VGVRKDGESWTQAKADGLQVATMLDAAKKAEIIQMLVPDEIQGAIYASEIAPNMTKGK
                     TLCFSHGFNIHYNQIVPPKDVDVIMVAPKGPGFLVRRTYTEGNGVPALIAIYQDATGK
                     ARDIGMAYAKGIGATRAGVLETTFKEETETDLFGEQVDLCGGVTCLMKASFETLVAAG
                     YQPEIAYFETFHEMKLIVDLIHEGGLSKMWYYVSNTAEYGGLTVGPKIINEQSKQAMK
                     EALKRIQNGEFAKEFVLESKANRPVLNALERQENEHQVEKVGKELRAMMPWLKKD"
     gene            5760..>6626
                     /locus_tag="VKL21_12480"
     CDS             5760..>6626
                     /locus_tag="VKL21_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46624.1"
                     /translation="MMNRKQIIGVAGLVGFVGVATYGFTPQVLTVFGVGWLFLPPVVV
                     AGMMVRGLQVELRESKASCKIQTRNISLAKEHLEKIKSIGKYNNNFSAAIRDIIRQTT
                     KKSSVSDDYLFRWLMKEVDGVLLPDNILNEIIDPYLINSMESLETFLNDKFFDWDIHI
                     SFKYDNNVSPCNVVVDIKGCGEEGDSWGWGQKIRMTASIISQFLVKNSQVIPLKISCV
                     DLDEANELSGCGSVEFSSSDRQEAMKSLVRFFGENEERNKILREMGSGSNVNFWKSIV
                     DLHVSSGYNMVTI"
BASE COUNT         1803 a         1422 c         1339 g         2062 t
ORIGIN      
        1 cccaccattt catccttcag gttcaacacg ggcactccac caatgttctt atcgagaagg
       61 gtcgatatta cttcagttat cggggtattt gttcgaacag atatcacatt gcgggtcatg
      121 atgtcaccca cgatgagatt ccggatcctc tcatcctgct ggttgtcagg cacaagatcc
      181 ctgaaattca tcatcgcttt tgcgatatct ctttcagtga taattcctac gatatcgcca
      241 ttttccacaa taggcagccg tccgatatcg ttatccagca taatacgcct tccgtgggaa
      301 actctgtcgc caggacttac ggtaattggc tctttcatga tctgggctgc atatcccttt
      361 acacccctga tatttttcat gatctcatga ggagttaccc agcccaggat tttactattt
      421 tccatagcga taaggattcc gccatttcgg tccatcaata ctatcgcatc ctcaataccc
      481 atatcaggga gtactttggc gaatttatcc gttgttgcgg ctgcaacatg cagcgatgat
      541 gcgggaagat tggaagtctt ccaggtcccg agtttgtctg caatgcttct catagtgata
      601 acacccatcg cctcgcttcc gttgatcaca agcagccttc gagtatcatg cttatccatc
      661 aggtccatcg catgtgaaat cttatctgat ttatgcacag taaatggttc gcacattatg
      721 tttttgattt tcatggtatc atctcacatt gtcattatag tctttataat attctcccct
      781 gtcaatattc ccatgacacc attgcctatc acagaaagac cattgatcct ttcttttacc
      841 atcaggcccg cagcaacagt cgcaagctct tcactattta cattgacaat cgggcttgac
      901 ataatatctt ccgcgaccag gggcacttcg tcaacatacc tgttctgttt tcttcccgca
      961 gagctatctt ttcgcgtcat ttttataact ttctgtggga ggccacccgt tttgtccttc
     1021 atttccgtga aggtgagatt tgaattggtt ataatcccca caggcttatc attatcctca
     1081 agaacaattg ccctgtcaat tgaatttgcc tgaagctcgt gtattatgtg gcttattgta
     1141 tgatgcctgt ggacagtcag tgcgggttcg attttcatat cctttacttt caggtcaagc
     1201 tccagttttg agaaatacct tatcaaatcc cattttgtag ctattcctat gacttcgtct
     1261 ttgttgttta ctaccggtaa cccacctata ttattctcgg tcatgagttc ggccagttgt
     1321 ctgggtgtcg catcagggaa tatcgttata aggctctcgg tcatcacttt ccttacaggt
     1381 atatcatcga tggggcgcct gcgccattgg ggttctgcct ggttaagcct ttttacgata
     1441 tctttcttgg tgaccattcc tatgggttta ttatcctcaa taattacaag tctcccgatt
     1501 ttatggcgaa gcatcagatt tcttgctctt gccaccgttt catcaggcga tacaacataa
     1561 acaggtgcgc tcattatatc ttttattttc ataaatacct cttaattagc cattgcccgg
     1621 acaaaatcac gttcggttat aattcctttc aattcgccct cttccagtac aggcagtgag
     1681 cccacatcct tatcgagcat cacatttgca gcttctccaa ggtcaatatc cgacttcgta
     1741 aaaattatgt ctctttgtat aagagtgctg accgaaactt caaaaacctc tctggcattc
     1801 cctgtgacaa gccgatcgaa catgtcaccg cttcccagga aacgcataat atccgaagca
     1861 gtaataatgc cgatgagtac attatcccga aggatgggaa gtctcctgaa accattattt
     1921 atcatggatc tggcggctgc ccctatagac atatcagaag gcgcggtcac tattttttta
     1981 ctcatatatc cttcaacggt cttacccgta gcgataccag acatcaggaa aacaaaatcc
     2041 ctctcggaaa ttatagcttt aacagcgcca tccccatccg ttatagggat ccctccgatc
     2101 ttctctttta tcatagtgtt caatgcatct ttcaatgaat ctttaacatt aagggataca
     2161 acatttgttt ccatgatctc gctaatgctg ccattgacgg ctgctagcag atttcccttg
     2221 aacttattct taattatcag atttctcttt ccgcctccaa ggaaatccac aatgtcattt
     2281 gacgtaacga tacctaacag gtgtttggtc ccggcgtctg ctatgggtat tcttctaaaa
     2341 ccgtgattca gcatggtctt tactgcctct atcacaggca tcgtaggggg aatagtgata
     2401 acatcgtttg atgcaatact cattatatcc ccggattttt cggatatcct tgatttgaaa
     2461 tctatgggtg ccatgttccg catatatccc tttcgaccct gtttttcaat tcttatatta
     2521 ttgccagatt tattcataaa aacaccttta gatgaatgct ttaattatat cattcctgtc
     2581 aactatgcca acaagttttc cctcgttggt tattgagatc ctccccacat caagtttcag
     2641 gaatgactgt attgcttccg caagcgtggc ttcggaagat agtgtgtatg ccggagtgct
     2701 cataattttt tcaacaggtg gagacatcgt agatttcgtc ccctggttgt cttcccgttc
     2761 gatccttgca taccccgcat tgattatatt acgccgtgtt accattccca caagttcccc
     2821 attttttaac accggaaatc ctgatattcc ggtttctttc atgttcagcc atactttgga
     2881 tatatggtct ttgttagaac aagtttttac atccctggtc attatttcat gaagcttttt
     2941 ttgggggatc ttatctacct tcaggttccg gaagatgtca acaacgctca aaattcctgc
     3001 aagcgtcatt tcctgtccgg atttcagaac aggagatcgc cctacaccgg catccatcat
     3061 taatttggcg gcatccatca tgtccatttc cggatatatg aaagggaatt ctgatgtgaa
     3121 accttcaacc gttacgtttg atttagttga atatatattc agtaactctt tttccgtcag
     3181 gatccccaat accctattct ttgaatcaag taccggcata gtcctgtaat gcctgtccct
     3241 catcacctgg cgcgcatgcg tcataaaatc cgtatcctgc aaaaaaacag gattcctggt
     3301 cattacgtct ttaactttca taatatactc cttttataac gtataattat gttatttgtt
     3361 aatttattca aaatcctttg taagctgcta tttcaagttt ccctgcagct ctcacagaga
     3421 agttttcctt ctatcgctaa taaattatcg gatatgtatc cgcaatcttc gcagattccc
     3481 ttaaaaacac gtccttccgt ctccatggta aataattttt ccctgtgcat cttgatcagg
     3541 tctgaatata tgctgcccat ttcagccgaa acagcgagta gatcagtgtc cgtaataatt
     3601 cccaccagtt ttccattttc aacgaccggc aatcgccgta tctccatttt caccatttta
     3661 tgcattgttt cggaaacatc ctctgaagag gatatggaaa taagaggagt ggtcattaat
     3721 tctttaatgg aaatttcatc cggtcttaga ttctttgaaa cgatcttttt tactatatcc
     3781 cgttccgtaa ctatccctac cggttgttta ttatcagtga ctatgacgct tcccaccttg
     3841 aatgttagca ttttcactgc taattgccgg acatcacttt tgatgtccac agttacaaca
     3901 tccccggtca ttatttcttt cacaggcatc ttaacttcca tgttttgggt cctccctaaa
     3961 gtataactat gtattttgaa gtgtttatat gttatgctgg ggcgatttga gaggatttac
     4021 tgttttagca tcctgaccat tttaatagca gcttcaacgg ctcttttgcc atattccact
     4081 ctttcatgag catccagcct gctcataccg ggacctgaaa tcccgagagt tattggtttt
     4141 gaaaattcaa gcgcaaggtc tgcaatcttt cgtgaagcat gctggatcac gagttcatcg
     4201 tgttttgtcg ctccctgaat aacgcaaccg atcgtaacaa ccgcatcgat tgttttgtcc
     4261 tgcgccattt tttttacagc caggggcata tcaaaaactc ccggtacata atatattttc
     4321 tcaaccgtgg cgccaaggaa cgccgcatgt tcttttccga gcgtctccat ctggatagta
     4381 agatcacggt tgaattcaga aacaacaaat cctaatttta tagtcatttt ggcacctggt
     4441 ttgttatatt atttggaaca tctatatatt tttcgataat gtagtatccc gatatttttc
     4501 gataatgaaa catttatatt ccttatcaat gttatgaaag cccaagaagg tatctaatat
     4561 ggtgaaaata tactatgaca aggatgcaga tctaaaagta ttgaaaaaga aaacaattgc
     4621 cgttattggt tatggaagcc agggagcggg gcaggcacag aacctgcatg attccggact
     4681 taatgtaatt gtaggagtaa ggaaagacgg ggagtcctgg acgcaggcaa aagctgatgg
     4741 cctgcaagtt gcgaccatgt tagatgctgc aaagaaagct gagatcattc agatgctcgt
     4801 acccgatgag atacagggtg caatttatgc ttctgagatc gccccgaata tgacaaaggg
     4861 aaaaacattg tgtttctcgc atggtttcaa tatccattat aaccagatcg ttccgccaaa
     4921 ggatgttgac gtcataatgg tcgcacccaa gggcccgggt tttcttgtac gcaggacata
     4981 tacggaagga aacggtgtcc cggcgcttat tgcgatctac caggatgcga caggaaaagc
     5041 aagggatatt ggtatggcct atgcaaaagg aatcggtgca acaagggcag gcgtgcttga
     5101 aacaacattc aaggaagaaa ctgagaccga ccttttcggt gaacaggtgg acctgtgcgg
     5161 aggagtaaca tgtctcatga aagcctcatt cgaaacactt gttgccgccg gataccagcc
     5221 ggagatcgct tattttgaaa cattccacga aatgaaactt attgtagacc tgatccatga
     5281 aggcggattg agcaagatgt ggtattatgt gtcaaatacc gcagaatatg gcgggctgac
     5341 agttggtcca aagatcatca atgagcaatc caaacaggca atgaaggaag cgctaaagag
     5401 aattcagaac ggtgaattcg caaaggaatt cgttcttgaa agcaaggcga accgaccagt
     5461 actgaatgcc cttgaaagac aggagaacga gcatcaggtc gaaaaagtcg gaaaagagct
     5521 tcgcgccatg atgccgtggt tgaagaaaga ttaagatggc aaattgccat cttaaataaa
     5581 ttttttggat attgacaaat caaagtgacc ataatctttt ttaattatat cgagcaatac
     5641 aatattgcat attgggaaaa tgtgatttcc cttttccagg ctatctgtcg aagtcaagtt
     5701 gaaaaaaagg actttccaat agcagagaga gcaaaatgta gtcgtctgag atgatgaata
     5761 tgatgaatag aaaacagata ataggggtgg ctggactggt tggattcgtt ggagtggcta
     5821 cctatggctt tacaccgcag gtcttaacgg tttttggggt gggatggtta ttccttcccc
     5881 ctgttgtcgt tgcgggtatg atggttcgtg gattgcaggt agagttgaga gaatctaagg
     5941 cttcgtgtaa gatacaaact agaaacattt cgcttgcaaa agaacattta gaaaagataa
     6001 aatctatagg aaaatataac aataatttca gtgctgcaat aagagatata atcaggcaaa
     6061 ccacgaaaaa gagttcggtg tcggacgatt atttatttag atggttaatg aaagaagtag
     6121 atggtgttct tcttccggac aatattttga atgaaattat agatccatat ttaataaatt
     6181 cgatggaaag tctggagaca tttctaaacg ataaattctt tgattgggat attcatatat
     6241 cttttaaata tgataataac gtgtctccgt gtaatgtggt cgtggatata aaaggttgtg
     6301 gagaagaggg agatagctgg ggatggggac aaaaaataag aatgaccgca tcaataatat
     6361 ctcaatttct cgtaaaaaat tcacaagtta ttcctctcaa aattagttgt gtagatttgg
     6421 atgaggcgaa cgaattgagt gggtgtggat cggttgagtt ttcgagctcg gataggcaag
     6481 aggcgatgaa aagtttggta agattctttg gggaaaatga ggaaagaaac aaaatattaa
     6541 gggaaatggg gagcgggagc aatgttaact tctggaaatc tattgtcgat ttacatgttt
     6601 caagtggata taatatggtt actatt
//
LOCUS       DATGBN010000487         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_504421, whole genome shotgun sequence.
ACCESSION   DATGBN010000487 DATGBN010000000
VERSION     DATGBN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504421"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..178
                     /locus_tag="VKL21_12485"
                     /pseudo
     CDS             89..178
                     /locus_tag="VKL21_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(542..1090)
                     /locus_tag="VKL21_12490"
     CDS             complement(542..1090)
                     /locus_tag="VKL21_12490"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HMB46625.1"
                     /translation="MEWPVTSGDYITGDVNSSVAVVTLASDYRAWSLKDYAICGTCFT
                     ENFGIEKVIVNVLANSNINCLIVCGEESNHLTGQSMISLAENGVSTMGNLRKIVGSNS
                     PLPYLNEIPMTAISRFLREIKVIDLIGNKDPATIQKAIDSCTTHARSEAYVASMPEID
                     ENTWRKYEKLVTQNVMSKIKKG"
     gene            2009..2155
                     /locus_tag="VKL21_12495"
     CDS             2009..2155
                     /locus_tag="VKL21_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46626.1"
                     /translation="MEKDTLSEEKDSENSPKDDEEDLLYMHKPESRGSTMQWNSSLCR
                     RPGH"
     gene            2303..2644
                     /locus_tag="VKL21_12500"
     CDS             2303..2644
                     /locus_tag="VKL21_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMB46627.1"
                     /translation="MSFLNEVLPETSKVFGQMQNSIFKDGYLDLKTKELIAVASSVIM
                     RCQFCVDAHFQRAVAAGATKEDIAEAVSVAMFIAAGSQIGWTKVYGENIYDRIFEKDK
                     EEEDCSCCCGN"
BASE COUNT          869 a          483 c          524 g          929 t
ORIGIN      
        1 gttaatatat tgaaggaaga actggatgag ttttggggga agcatacatt cacacacaaa
       61 atgtcgcatt atttaaaatg gtgactataa ttcctttaaa tggaagcatt atgaaggaga
      121 aattatccta ttgaatgtca gatggtactt aaaataccca ttgagctata gaaatttaaa
      181 agcaactgta tagaaaattc tacgtactgc cagacttcat tttcttttaa aaatgtttta
      241 cctcatttat gatggaaaca gcagtgtttt aggatcatgt tgctacattg attttagtaa
      301 aacctatgta aaacaggcat ttttaagtta aataccttta aaatataagg caaaatcccc
      361 taaaatacac aggttatatc acaaaaccta tgcatcgaat aaaccgcaaa aatacagagg
      421 tttcacagca aaacctatgc ataacatgta gagtgaattt acagaaaagt tgatacactg
      481 ccaaaaattc aattgcagga tttctacaga gcttgatttt tcttttccgg gctcttccag
      541 ctcatccttt ctttattttc gacatcacat tttgagttac caatttttcg tattttctcc
      601 aggtattttc atcaatttca ggcattgatg caacataagc ttcacttctc gcatgagtgg
      661 tgcaggagtc gatagctttc tgaatagtgg caggatcttt atttccgatc agatctatta
      721 cttttatttc cctgaggaag cgggagattg cagtcattgg aatttcattc aaatagggca
      781 aaggagagtt ggagcctact attttccgta agtttcccat tgtggaaact ccattttcgg
      841 caagagaaat catagattgg cctgtaagat ggttactttc ctcaccgcag acgatgaggc
      901 aatttatgtt cgaatttgca agcacattga cgattacttt ttcgatcccg aaattttctg
      961 taaaacaggt cccgcaaatt gcatagtcct tgaggctcca ggctctgtag tcagaggcaa
     1021 gcgttactac agctacgctt gagtttacat ctccagttat ataatcacct gaagttacag
     1081 gccattccat aatatcgctc ctaccgttaa ctcattgtta aaacagataa ttaattgcta
     1141 ctttcctact ttaaactact ttctggagct cattctataa attttagttg aaatattttc
     1201 tttattgtct tttaggattt tgcgtacttg aaatagaaaa ataatgcttc aggcgtcata
     1261 tttaaaattc tattttaaat agctaaaagt ttagtgccat tgatttctca gctgtaagtc
     1321 ctatgagatg tacttatgat cttatttttt tgcaacatgc taggatgttg gtggtatctt
     1381 tggtgtagta ttgggagccc ttgttttgct atcttcccag taaatggagt gactatgaag
     1441 aggtcaggga gtgagacaag tatatcgctc tctcctagcc tgatggtttc tggctgactc
     1501 ttgctattct tattggtgta ctctctggag cagtccccgc atacagggct tcaaaactaa
     1561 aacatgggga tgctttaggg tatgaataac agaaaagtaa agagatcgtc ccttcttaat
     1621 tcactttcat ctccaaattt ttcgaatatg cattttccgt tatcaatttt atcaattgcg
     1681 aattaattga acataaaatg tgcgattcaa aactgataga gggttggata tagagatcaa
     1741 aacaataaca tgcaattatc accaaattca atacatctcc tcatatttcg agagtcttct
     1801 gttcttgtta tttagctttc gggactttat gttagtacct acgcaaaatg atattgtaga
     1861 ttatctttct cgaatttaaa gaaagcgaaa aggtatattc actatatatc aattattctt
     1921 gatacccata ctgaaggttt ttaaaacttg ggtctccaaa taacatttac atcgattttg
     1981 gaaattaatg aatcaaggag gataggaatt ggaaaaagat acattatcag aagaaaaaga
     2041 ttctgaaaat agcccaaaag atgatgaaga agacttactg tatatgcata agcctgaatc
     2101 tcggggtagc actatgcaat ggaattcttc tctttgtaga agaccaggcc attaagaata
     2161 tggtatgacg cctgaaaacc tattatttta aaatgaaagc ctaaaaaata ttctggcctt
     2221 tttgtttgtt aggagggtcg tagaccagct ttaagtcaaa agggttttaa ctgtaccata
     2281 ttctaaacta ggggatgttg agatgtcatt tttgaacgaa gtattacctg agacttcgaa
     2341 agttttcgga cagatgcaaa attccatttt taaagatggc tatctggatc tcaaaacaaa
     2401 agaattaatt gctgtcgcct cttcggtcat catgcgttgc cagttctgtg ttgatgccca
     2461 ttttcagaga gcagtggctg caggggctac aaaggaggat attgcagaag ctgtgtctgt
     2521 tgcaatgttc attgctgccg gttcacaaat agggtggacg aaagtatacg gggagaatat
     2581 atacgacaga atttttgaaa aggataagga agaagaggat tgcagctgtt gctgtggtaa
     2641 ctgaaattcc aaggtttgta ttttatcagc tattcccgtt ttagttaacg cgatcttcat
     2701 ttaacttact ttttttgatt ttgtcttcga tatatgtaca tatacagtac ttcaccaact
     2761 ggatcagaaa atcatctatc cccattacga tttcaaacat ttaac
//
LOCUS       DATGBN010000488         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_77389, whole genome shotgun sequence.
ACCESSION   DATGBN010000488 DATGBN010000000
VERSION     DATGBN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77389"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..293)
                     /locus_tag="VKL21_12505"
     CDS             complement(33..293)
                     /locus_tag="VKL21_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46628.1"
                     /translation="MEIHKDKQQENFTLVVTRDELIEIRDALGLRGLYLEALGRKKVK
                     IARSKDVADANVVARGIQKDKSRNMYMQIVKEAQFHFLVIIK"
     gene            complement(306..1064)
                     /locus_tag="VKL21_12510"
     CDS             complement(306..1064)
                     /locus_tag="VKL21_12510"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMB46629.1"
                     /translation="MGRLVLDSVTKKFNNFLALVNVSFEVADGEFVCLLGPSGCGKTT
                     ILRLAAGLEAPIGGNLFLDGKKIEGVNKECGFVFQEYALFPWRTVKENIEFGPQAKGL
                     HKNECDRISKHYIDLVGLNGFENHYPKELSGGMKQRVGIARAYANSPKLLLMDEPFGA
                     LDAQTRNLMQGELLRIWEKEHISVLFVTHSVDEAVFLGDKVVVMSARPGTVKEIFYVK
                     LPRPRIRTSLEANIIRDRVLKSLGMEIQLAMKDK"
     gene            complement(1052..1858)
                     /locus_tag="VKL21_12515"
     CDS             complement(1052..1858)
                     /locus_tag="VKL21_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMB46630.1"
                     /translation="MKIDTRKKELLINLLSLVGFVLLWQAVLILTRQGILNIPFIRLR
                     DIPTPVETWHALLGSMFTHVYGPAITYGIYNHVLASLERVIEGGIIAFIIGVPVGIGV
                     GYSKLIGNIFNPIIEIVRNMPPMAWIPIAIFIFVSGGSIFIVFIGVVFPIILNTIQGV
                     RSTDERLLDAARTLGASESMIVTKVVIPSAFPSIMTGLRIGLGVGWMAIVAAEMVIRS
                     PVGLGYFIWNMGDLGRYAEMIAGMIVIGVIGYMMNISILLLQRRFIKWVD"
     gene            complement(1858..>2610)
                     /locus_tag="VKL21_12520"
     CDS             complement(1858..>2610)
                     /locus_tag="VKL21_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMB46631.1"
                     /translation="VEYINNAKQSDSKDHEELINFTMQFGGMTDPAAANLSLADVEFV
                     YNTNIPDTTTFIQKIQDFGIFVPEKWNQSGYKYASDYANSLVTNKYVDWAINNKDVNS
                     SNISLKEPVTVRYGYLINDIHELPFYVGWQKGYYRDMGINISVAQGAPFQNGAFEMQK
                     GFKANTIDIGSLGIPPVIIHRINSNDFSNDDTRVGVIAGMNNEGSVIVVASNITSLKD
                     LSGKTVGYPGPGTIQHVLFLMEADKEGLKVSY"
BASE COUNT          708 a          636 c          475 g          791 t
ORIGIN      
        1 aattatattt tattatttac aaataaacat tgttatttaa ttatcactag aaaatggaac
       61 tgtgcctctt ttactatctg catatacata ttacgtgatt tgtccttctg tattcctcgc
      121 gccaccacat tcgcatcagc cacatctttt gacctggcaa ttttaacttt cttccttccc
      181 agcgcttcaa gataaagacc tcgtagtcca agagcatctc ttatttcaat gagctcatct
      241 cttgttacca caagagtaaa gttttcttgt tgtttgtctt tatgtatttc catgttattt
      301 gtcccttact tatccttcat cgccagctgt atctccattc ccagcgactt tagcaccctg
      361 tccctgatga tatttgcttc aaggcttgtg cgtatccttg gtcttggcag ctttacatag
      421 aagatctctt tgaccgtacc cgggcgggct gacatcacca cgactttatc gccaaggaaa
      481 acagcctcat ccacgctgtg ggtgacaaaa agcacgctga tgtgctcttt ttcccagatt
      541 ctcagtaatt ctccttgcat aagatttctt gtttgagcat caagcgcccc gaaaggttca
      601 tccatcaaca gcagtttcgg gctatttgca tatgcacgtg ctatcccgac cctttgtttc
      661 attcctcccg ataattcttt tgggtaatgg ttctcaaatc cgttcaagcc cacgaggtct
      721 atataatgtt ttgagatccg gtcgcattca tttttatgta atccttttgc ctggggaccg
      781 aattcaatat tttctttcac agtcctccag gggaacaacg cgtattcctg gaacacaaag
      841 ccgcattcct tattcacccc ttctattttt tttccgtcaa ggaacaaatt acccccgatc
      901 ggtgcttcaa gtccggctgc aagacgaagg attgttgttt tcccgcatcc acttgggcca
      961 agcaaacaaa cgaactcacc gtctgcaact tcaaaactga cattaacaag tgccaggaaa
     1021 ttattgaatt tcttggtaac agaatccagt actaatctac ccacttgatg aacctcctct
     1081 gtaaaagaag gatgctgata ttcatcatat atcctattac gcctattact atcatacctg
     1141 ctatcatttc ggcgtaacgt cccaggtccc ccatgttcca gatgaaataa ccaagaccca
     1201 caggacttct gataaccatc tccgcagcaa caattgccat ccatcccact ccaagtccga
     1261 tcctcaatcc ggtcataatt gatggaaaag cagaaggtat aaccactttt gttacgatca
     1321 tcgattcaga agctccaagt gtcctggcag catccaggag ccgttcatct gttgaccgga
     1381 cgccctggat agtgttaagg attatgggaa aaacaacacc tataaaaaca ataaaaatcg
     1441 aaccacctga tacgaaaata aatatcgcga tcggtatcca tgccatgggc ggcatatttc
     1501 ggacaatttc aattatcgga ttaaatatat tcccaataag ctttgaatat ccaacaccga
     1561 tacccaccgg caccccgatg ataaaagcaa ttatccctcc ctctatcaca cgctccaggc
     1621 ttgctaaaac atggttgtaa ataccataag taatagccgg accatagaca tgggtgaaca
     1681 ttgaaccaag aagcgcatgc caggtttcga ctggtgtggg aatatcccga aggcgaatga
     1741 agggtatatt taaaatgccc tgacgcgtaa ggattaagac ggcctgccat aaaagcacaa
     1801 aaccgacaag agaaaggagg ttgatcaaca actccttttt ccttgtgtcg atcttcatta
     1861 ataactgacc ttgagtcctt ccttatctgc ctccataagg aaaaggacgt gctggatagt
     1921 tcctggtccc gggtagccaa cagtctttcc gctgaggtct tttaatgatg ttatgttact
     1981 tgcgacaaca attactgacc cttcattgtt cattccggca attacgccca cacgggtatc
     2041 atcatttgag aaatcattgc tgttgatcct gtgaatgatc accgggggta ttccaagact
     2101 tccgatatcg atagtatttg ctttaaaacc tttttgcatc tcgaacgcgc cattctggaa
     2161 cggggcgcct tgcgctacac ttatgttaat tcccatgtct ctataatacc ctttttgcca
     2221 tccaacgtag aatggaagtt cgtgtatgtc attgatgagg taaccatagc gaacggtgac
     2281 aggttccttc aaagaaatgt tggaagagtt cacatcttta ttattgattg cccagtcaac
     2341 atatttattg gtaaccagtg aatttgcata atctgaagcg tacttatatc ccgattggtt
     2401 ccatttttcc gggacgaaaa taccaaaatc ctgtatcttc tggataaacg tggttgtgtc
     2461 aggaatattc gtattataaa cgaattccac atcagcaaga ctcaaatttg ctgctgccgg
     2521 gtctgtcatt cctccaaatt gcattgtgaa gtttatcaat tcttcatgat cttttgaatc
     2581 gctctgcttt gcattattga tgtattcgac
//
LOCUS       DATGBN010000489         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_543507, whole genome shotgun sequence.
ACCESSION   DATGBN010000489 DATGBN010000000
VERSION     DATGBN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543507"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..894)
                     /locus_tag="VKL21_12525"
     CDS             complement(169..894)
                     /locus_tag="VKL21_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HMB46632.1"
                     /translation="MEIISDIISKTVPGIRMLFDMKNVIYDRDWLTSAKNIELYYMYR
                     ELSLGKKDALLIKEHGLRYDITVIPPRMLGCEFVKTAGHYHPLVPGTQITFPEIYEVL
                     GGDATYILQKPAGEGIEDVILIKAGTGDKVIIPPGYGHLTINASNKILKMANWVAHDF
                     ESIYQPIEEKCGGAYFILDKGMVKNTRYEHVPEIKFLKPSNLEEIGLQKNKEMYGLVR
                     DLKKLEFLTKPHEYEWVFEKVLL"
     gene            1013..2362
                     /locus_tag="VKL21_12530"
     CDS             1013..2362
                     /locus_tag="VKL21_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMB46633.1"
                     /translation="MQKILLIILSYLSISGSALAISEITLNEIKRPQSAILFIVDGFG
                     SSYFYPEYSPSALDGSLLLKANTYNMSFGTRIEDIRTRHPVTGIAHSVIVTGFSDANE
                     ETVGYPDATIFDITKQHGFVNLAVMETGDSVNMRNEQDIILFAENNSIDEPLMPIQSK
                     SAPAGVYDFMYEWKMKLPAYLDGKKGVDKYSAYNRWCIDAANAAALDMIQKHPSQRFL
                     LTVNIAGIDSGGHNMGYDDYIRLIESLDSDIYPLYKTALDNNIAFFLTADHGMSFAAI
                     NARRGGHSSDKYSTQLESLRIPLVILSPNAATGIIEGEFHQEDIAPTLLGVLDLPDHL
                     QYVDGNAINVKNYATIFVEADPSYQVSLWNNGTKLSERSDSELIFTGLPLDSSYTLKA
                     TGKYGTFEEEVSLDSDKHFNFKKPDRAENISNRGLIAIILILIVNIAGLIIIRRIKD"
BASE COUNT          843 a          543 c          521 g          835 t
ORIGIN      
        1 tccctccaga aaaccctgtg ataccccgga aaatagatca tgtccgtggt taatgaaata
       61 acacatcctg ggacatttat tgaatgtggt gatgtctgaa acccttatca tggaacctgt
      121 aaatatcccc ttcatttaaa aatattgctg caaatttgat tattggtttt ataaaaggac
      181 tttttcgaaa acccattcat actcatgagg ttttgtgagg aattcaagct tctttaaatc
      241 acgcacgagc ccgtacatct ctttgttttt ctgcaatcct atttcttcga gattcgaagg
      301 cttaaggaac ttgatttcag gcacatgttc atatcgcgta ttttttacca ttcctttatc
      361 cagaatgaaa tatgcccccc cgcacttctc ttctattggc tggtatattg actcaaagtc
      421 atgcgccacc cagttagcca ttttcaatat cttatttgat gcattaattg taagatgccc
      481 atatcccggc gggattatta ccttgtcgcc agtgcctgct tttattaaga tcacatcttc
      541 tataccttca cctgccggtt tttgaaggat ataagttgca tcaccgccca gtacttcata
      601 tatctcagga aatgtgattt gcgttccggg gacaagagga tggtaatgac ctgctgtttt
      661 tacaaattcg caccccagca tgcgcggcgg gatgacagtg atatcgtacc tcagtccatg
      721 ttcttttata agcagcgcat cttttttccc cagtgaaagc tcacgataca tgtaatataa
      781 ttcgatattt tttgcactgg taagccaatc acggtcataa atgacatttt tcatatcaaa
      841 aagcatcctt ataccaggca cagtcttaga tatgatatcc gaaataatct ccattgctta
      901 ctaattggca ttttaatata taaattatct gattagctat aatttcaaga aaataaatct
      961 tatttttttt ttcgcaatct ttttatttga ttcctgttta tcaacaaata ttatgcaaaa
     1021 aatcctgtta ataatattat catatctttc aatatccggc tctgcgttag ctatttctga
     1081 gatcacgttg aacgagatca aaagaccaca aagcgctatc ctgtttattg tagatggttt
     1141 tggctcttca tatttttatc cggaatattc accttctgca cttgatggtt ctttgctttt
     1201 gaaagcaaat acctacaaca tgtcattcgg gacaaggatc gaggatataa gaaccaggca
     1261 tccggtcaca ggtattgcac attcggtaat agtaacggga ttttcagatg caaatgagga
     1321 aactgtaggt tatccggatg ccacaatatt tgatatcaca aagcagcacg gttttgtaaa
     1381 tcttgctgta atggaaacgg gagattctgt gaatatgcga aacgaacagg atataatcct
     1441 gttcgccgag aataattcta ttgatgagcc gctaatgcca atacaatcaa aaagtgcacc
     1501 tgcaggcgtt tatgatttta tgtatgagtg gaagatgaaa cttcccgctt accttgatgg
     1561 aaaaaaagga gtggataaat attccgcata taacaggtgg tgtattgacg ctgcaaatgc
     1621 cgctgctctc gatatgatac aaaaacaccc ttcgcaaaga tttctcctga cagtgaatat
     1681 cgcgggaatt gacagcggtg gccataatat gggttatgat gattacatca ggctcatcga
     1741 gagtctcgat tcggatattt atcctcttta taagactgct cttgacaata atattgcatt
     1801 ctttttgaca gcagaccatg ggatgtcttt tgcggcaata aacgcgcgaa gaggagggca
     1861 tagctcggac aaatattcaa cacaacttga aagtcttaga ataccgcttg ttatactatc
     1921 cccaaatgcc gcaacaggaa ttattgaagg ggaatttcac caggaagaca ttgcacccac
     1981 gcttctgggt gtacttgacc ttcccgacca tctccagtat gttgatggaa atgccataaa
     2041 tgtaaaaaat tatgccacta tttttgtaga agccgatcca tcctaccagg tttctttatg
     2101 gaataatggt acaaaacttt cagagcgctc ggattcagaa cttattttca cagggctgcc
     2161 cctggattca agttataccc tgaaagcaac aggaaaatat ggtacattcg aggaagaagt
     2221 gtcgctggat tcggataagc atttcaactt caaaaaaccg gatagagcag aaaatatcag
     2281 taacagggga ttgatcgcaa ttatcctgat attgatagtg aatattgcgg gattgatcat
     2341 catcaggagg ataaaggatt aaccgataga attaagaaat tcatgagcac atagacaact
     2401 attgaataaa cccacagagt tgctcagacg gatcgctgaa ttggaaaaat tagaaactga
     2461 gcacaagctg acaatagaaa ttttgcaggc aagtgagaac aagtatagag tattactaga
     2521 aaatttgccg caaaaaatat ttcataaaga taaaaactct gtttatgtat cctgcaacaa
     2581 taactatgct cgtgatttga aaattcggtg gtgtcctaaa taacgtggcc tattcaaaaa
     2641 actgtcacaa tggtagcttt gagataatga ggtgtctttc aaaaaaagca ccctgtggct
     2701 caatgtcttg ctggttcatt tacacaccac atcatctcta tt
//
LOCUS       DATGBN010000490         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Methanoperedens sp. isolate SMAG_U9623
            k141_466103, whole genome shotgun sequence.
ACCESSION   DATGBN010000490 DATGBN010000000
VERSION     DATGBN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965194
            Sequence Read Archive: SRR5450757
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methanoperedens sp. (soil metagenome)
  ORGANISM  Candidatus Methanoperedens sp.
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Methanomicrobia; Methanosarcinales; ANME-2 cluster; Candidatus
            Methanoperedentaceae; Candidatus Methanoperedens.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 08:56:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,473
            Genes (coding)                    :: 2,456
            CDSs (with protein)               :: 2,456
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 6 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Methanoperedens sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466103"
                     /isolate="SMAG_U9623"
                     /isolation_source="soil"
                     /db_xref="taxon:2032688"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="68.6137 N 149.3144 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="iscB"
                     /locus_tag="VKL21_12535"
     CDS             <1..705
                     /gene="iscB"
                     /locus_tag="VKL21_12535"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HMB46634.1"
                     /translation="TEHAKQNTSVSAVYVLNMRGQPLMPTTPGKAKKLLKEDKARVIK
                     RTPFTIQLKYATGETKQEITLGVDSGFENIGLSAITAKKELYSAEVKLRTDMVKLNSE
                     RRQYRRARRNRKTWYRPPRFENRKKPEGWLPPSIQHKLDSHIKLINKVKQRLPITKID
                     IEVAAFDIQKIKNPEISGKEYQNGVQKDSWNAREYVLHRDNHTCQACKGKSKTRYLKL
                     IILFQDKSVGTHRITS"
     gene            895..1077
                     /locus_tag="VKL21_12540"
     CDS             895..1077
                     /locus_tag="VKL21_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMB46635.1"
                     /translation="MGLEKYHSTDAFVIAGGTMQERSSITFLIKQVRKCNRKLFKGER
                     SHIKNTAARIIHGFQR"
     gene            1325..1884
                     /locus_tag="VKL21_12545"
                     /pseudo
     CDS             1325..1884
                     /locus_tag="VKL21_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT          858 a          406 c          469 g          652 t
ORIGIN      
        1 acagaacacg caaagcagaa cacgagtgta tctgctgttt atgttctaaa catgcgagga
       61 caacctctca tgcctacaac accagggaaa gcaaaaaagt tattaaagga ggataaagca
      121 agagtaatca aaagaacacc attcacaatc cagttaaaat acgcaacagg agaaacaaaa
      181 caggaaataa ctctcggagt agattcaggc tttgagaata ttggtctttc ggcaataacc
      241 gctaagaaag aattatattc cgcagaagta aaactcagaa ctgatatggt caagctcaat
      301 tcagaacgca gacaatatcg aagagcaaga cgtaaccgca aaacatggta cagaccgcca
      361 agattcgaga acaggaagaa acccgaagga tggttgccgc catcaattca gcataaactt
      421 gatagccata ttaaattaat caataaagta aaacaacgtc taccgataac gaaaatcgac
      481 atagaagtag ccgcctttga tatccagaaa atcaagaatc ctgaaatatc agggaaagaa
      541 tatcagaacg gagtccagaa agatagttgg aatgccagag aatatgtgct tcatagggac
      601 aatcatacct gtcaggcatg taaagggaaa tcaaaaaccc gatacttgaa actcatcata
      661 ttatttcaag acaagtcggt ggggactcac cggataacct cttaacgcta tgcgaaacat
      721 gtcatgataa agtttcaaaa ggtaaattga aactggatgt taaattaccc acaggtttca
      781 aaccggaaac attcatgtca atcgtaagat ggaagctggt caatatgttt aagagatgtt
      841 ggaaatatcg taagtcatac ttatggatat atcacaaaat ttgatagaat agcattgggt
      901 cttgagaaat accatagtac cgatgctttt gtaatcgcag gaggaactat gcaggaacga
      961 agttcaataa catttttaat aaaacaggtc aggaaatgca accgtaaatt attcaaaggc
     1021 gaaagaagtc atatcaagaa tacggctgct cggatcattc atggattcca gagataagat
     1081 aaagttctct ggaataatat cgaatgcttt gtatttggaa gaaggaaaac gggttatttc
     1141 gaattgagaa aacttgatgg aacaaaaata cacacatcag caaaggcaaa agaccttaca
     1201 ttattagaaa ctgctaatac gttattaata tcatatttga ggcggggtac gctcctccac
     1261 actttgaaaa gtgtggtttc cgctacccct gcaccccacg gagtattatg aaatcaatag
     1321 aataatgaat cacattgagg agtttgggat tactatctca cacaaaaaag cccttgattt
     1381 tggttgtggt gttggcaggt taacacaaga gttggcatgt aattttgaag aggtgtacgg
     1441 tgttgatatt gtgcacggaa atacaacaag tttggcgaga aatgtcaata ttttttgaat
     1501 gaaaagagtg atttaaaact attcaaaagc aacacttttg actttgtgta ttccagcata
     1561 gttcttcaac acatggaatc caaatacagt aaagagtata ttaaagagtt cttgagagtt
     1621 cttgtgccag gtggactggc tgtattccaa atcccaagtg agtcaaaagc gcctgttgga
     1681 aaatatcacg aacctgaccc cacaattgcg gtaatagaaa tgtatggggt caagaaagat
     1741 gagattctga atttgattag taaaaacggc ggaaagtgcg ttaaagtaca ggatgattac
     1801 ggtatacctg agtgggtaag ttatcgctat tatgtcgtaa aagagcgctc gccttggtat
     1861 aaatgcctcc ctggaattgg ataacatcgc tatccatccg ttgttgggag gtcaaaatta
     1921 ggtctaagag gaatattccg tcccatatcg atggttattg tagatagata gcaaatctgt
     1981 tagcaggaaa tatttacgag aagcttcggg gcatcaaagg ggtcatggca ctgctatatt
     2041 atgaaaaaag atctgtctga aatttatatt ttgcttcctc tcctttattc taaaatatac
     2101 acttttcaaa aatcaggtaa ctgtagaatc ccgatatttt atagaaaagc ttattacaaa
     2161 ctctgatgaa tatacaaaac caacaaaaaa aattacggaa tatgaagagc ttttctgatg
     2221 acatttgaat atttattaat tataattaaa tcaatcatat cccttccatt tctttttttt
     2281 attccgggat ttctggtaaa ttatattctt ttttctcata aatccattaa actcgactcg
     2341 tttttaatac tgcatattta cgttaatata attataacgt ctctt
//
